LOCUS       DAOTCW010000001        51624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_318_length_51624_cov_33.809537, whole genome shotgun sequence.
ACCESSION   DAOTCW010000001 DAOTCW010000000
VERSION     DAOTCW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 51624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51624
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_318_length_51624_cov_33.809537"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..443)
                     /locus_tag="P5335_00005"
     CDS             complement(6..443)
                     /locus_tag="P5335_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRZ73291.1"
                     /translation="MRRIIVDYAKLTNDILSMLVEKFPDGYEDSDIIRFRNAQNELIE
                     AVEVRTEDTIYLVKVSKKLATRIENFEEDEDLDVEVETIAPVKGLDLDDDIDEVDVDE
                     EDDDAPSDDDTEVDLLKDSGGSDDEDDDDDEPSDEDDDDDEDY"
     gene            complement(455..1429)
                     /locus_tag="P5335_00010"
     CDS             complement(455..1429)
                     /locus_tag="P5335_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HRZ73292.1"
                     /translation="MSKGPISQFIEKNYLHFNSAALVDAAKGYEQHLLENGKMMITLA
                     GAMSTAELGKSLAEMIRQDKVHIISCTGANLEEDIMNLVAHNSYKRVPNYRDLSPQEE
                     WDLLENHYNRVTDTCIPEEEAFRRLQSHLFDIWNNADSKGERYFPHEFMYQMLNSGVL
                     KQYYEIDPKDSWMVAAAEKNLPIVVPGWEDSTMGNIFASYCIKGEFKPTTMKSGIEYM
                     MWLADWYPKNSGGKGVGFFQIGGGIAGDFPICVVPMLYQDMEMHDIPFWSYFCQISDS
                     TTSYGSYSGAVPNEKITWGKLDIHTPKFIIESDATIVAPLIFAYLLDL"
     gene            complement(1520..2920)
                     /locus_tag="P5335_00015"
     CDS             complement(1520..2920)
                     /locus_tag="P5335_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HRZ73293.1"
                     /translation="MNTKYFDLINQTFYFPQEEFTLNKDNLQFHHIDLMKIVEQYGTP
                     LKFTYLPQISSNINRAKGWFRKAMEKNKYEAKYYYCYCTKSSHFSFIMNEAFKNNIHI
                     ETSSAFDINIVESLMAEGKINKNTYVICNGFKREQYIENIAKLINGGHNKTIPIIDNY
                     EELDLLQDKIDGKFKIGIRIAAEEEPKFEFYTSRLGIGYKNILPFYRKQIEENKKVEL
                     KMLHFFINTGIRDTAYYWNELLKCLKVYVALKKECPTLDSLNIGGGFPIKNSLAFEYD
                     YQYMIDEIINQIKISCEEAEVDVPNIFTEFGSFTVGESGGAIYQVLYQKQQNDREKWN
                     MIDSSFITTLPDTWAINKRFVMLAINRWNDTYERVLLGGLTCDSDDYYNSEQNMNAVY
                     LPKYNKEKPLYIGFFNTGAYQETIGGFGGLHHCLIPQPKHILIDRDENGILATEVFSE
                     QQSAEQVLSILGYEKK"
     gene            complement(3212..4279)
                     /gene="aroB"
                     /locus_tag="P5335_00020"
     CDS             complement(3212..4279)
                     /gene="aroB"
                     /locus_tag="P5335_00020"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRZ73294.1"
                     /translation="MQKIQATGYSINFNQNGYIFLNSYLEEKNYSKLFVLVDSNTYAN
                     CLSHFLAHIATDLEIEIIEIESGESHKTIDTCVQLWHSLTELGGDRKSLLFNLGGGVI
                     SDIGGFVASTFKRGIDFINIPTTLLAMVDASIGGKNGVDLANLKNQIGVINSPKAVLI
                     DSTYLETLPQNEMRSGLAEMLKHGLIFDKSYWEKFLNLNDFTTEDLDDLIYHSVVIKN
                     AIVSQDPTENGIRKALNFGHTLGHAIESYFLESSTKKTLLHGEAIAAGMILESYISMQ
                     KELISREEYSQIKSGIQAIFEPIDFEENDLQPIMDLLIHDKKNEYGKIQFALLDGIGK
                     IKINQYAENEWIINAFEDYKC"
     gene            4410..5585
                     /locus_tag="P5335_00025"
     CDS             4410..5585
                     /locus_tag="P5335_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HRZ73295.1"
                     /translation="MEKIFDNTQVAFSLKSDSELDRAYFLFKLIDNEPLVRIGTAVTN
                     FALKAHLPVEGLIRASVFDHFCGGVSEDDCMPVMEKMFTKKVCSVLDYSVEGKEDEHQ
                     FDAAMKKTLKIIEFAKLKDAIPFAVFKPTGFGRLDLYTKVGRKDPLNFEEHQEWDRVV
                     ARYEAVCKLAFEKDVSLLIDAEESWMQDAADELVTKMMQKYNKEKAIIFNTIQLYRWD
                     RLDFLKKLHEQAKREGFYIGMKLVRGAYMEKENQRAEEKGYKTPICATKEESDKMYDD
                     TLAYMLDNINQMAVFAGTHNELSSYKLMQLMAEKGLSNNDNRIWFGQLYGMSDNISYN
                     LAANGYNVAKYLPFGPVRDVMPYLIRRAEENTSVAGQTSRELMLIKKERERRKLQDD"
     gene            complement(5644..7062)
                     /locus_tag="P5335_00030"
     CDS             complement(5644..7062)
                     /locus_tag="P5335_00030"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRZ73296.1"
                     /translation="MDRRKFFRNGTLFTLGSAFLNPFETIAQHPDISTTLKGKKTKNI
                     IVLVSDGMSSGTLNMADIYLNRKTGKGSNWLNLYRENKVNRALMDMASASSIVTDSAA
                     ASSSWGSGFRVKNGSLNIGVNGEEYLPIWQKFKKAGKMAGCVTTVPITHATPAGFCAN
                     SKSRNSQPEIAESYLKIKFDVLMGGGLQYFDSSLRSDKKDVFSAYESNGYQVVKTRNE
                     MLATANDKPILGVFYEDGLPYTIDRENVKDLSNAVPTLAEMTQKAIDRMKNHKEGFVL
                     QVEGGKVDWAAHANDIAGLLYDQVAFDEAIKVAIDFAEKDSNTLIIITSDHGNANPGI
                     IYGSTSNDHFDSIQHFKHTNEWILNGFNPNSTLSQVKERIEFANGFALNDEEAKTILS
                     YYEGLKKEDSGLYNYKNLPYKAFAEMQKSRTSVGWISMDHSADYTELAMFGPGSENLK
                     PFIKNTDLHYFMLNAAEVENKF"
     gene            complement(7193..8401)
                     /gene="lysA"
                     /locus_tag="P5335_00035"
     CDS             complement(7193..8401)
                     /gene="lysA"
                     /locus_tag="P5335_00035"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRZ73297.1"
                     /translation="MNSSELVGLADEFGSPLYVYDATKIESQYTRLANAFSKVERLQI
                     NYAAKALSNISILKLFHKIGAGLDTVSIQEVKLGLAAGFKPAQIIFTPNGVSMDEIEE
                     VAALGVQINIDNLSILEQFGTKHPSIPVCIRINPHVMAGGNTNISVGHIDSKFGISIH
                     QLPHLLRIIDNTHMHINGIHMHTGSDILDIEVFLYAAEILFETAKHFKTLDFIDFGSG
                     FKVPYKKDDIETNVEELGKKLSKRFNQFEKEYGRPLTLAFEPGKFLVSEAGFFLAKVN
                     VVKQTTSTVFAGIDSGFNHLIRPMLYGSYHHIENISNPKGKERFYSVVGYICETDTFA
                     NNRRIAEIKEGDVLCFRNAGAYCFSMSSNYNSRFRPAEVLWYEGKGHLIRERETFVDL
                     VRGQIEITFL"
     assembly_gap    8727..8826
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8829..9989
                     /gene="sucC"
                     /locus_tag="P5335_00040"
     CDS             8829..9989
                     /gene="sucC"
                     /locus_tag="P5335_00040"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRZ73298.1"
                     /translation="MASYGVRIQRGIVANSPVEAVAAAKQLTAETGTGWHVIKAQVHA
                     GGRGKGGGVKLAKNLQQVEELAEQIIGMQLITPQTPPEGKKVHKVLVAEDVYYPGESE
                     TSEFYMSVLLNRGRGRNMIMYSTEGGMDIEEVAEHTPHLIFTEEIDPTVGLQAFQARR
                     IAFNLGLSGTAFKEMVQFVQSLYNAYIGSDASMFEINPVLKTSDNKIMAVDAKVNIDD
                     NALYRQAKIADMRDIREENPIEVEAKAAGLNYVDLDGTVGCMVNGAGLAMATMDLIKY
                     AGYEPANFLDVGGTADAKRVELAFRIILKDPNVKAILINIFGGIVRCDRVAQGVVDAY
                     KNMGDAIKVPIIVRLQGTNAEIAKELIDNSGMPILSAVQFQEAADQVKVALG"
     gene            complement(10094..10744)
                     /locus_tag="P5335_00045"
     CDS             complement(10094..10744)
                     /locus_tag="P5335_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRZ73299.1"
                     /translation="MKTALIIGSTGLVGAQLLNLILESPVYDKVVVFVKRETSIKHPK
                     LIQHLIDFDKIENYQNLVVGDDFFCTIGTTIKKAGSREAFKKVDLIYPKQFAQIALKN
                     KVSQFLIISSLGANEQSTNFYLKTKGEMEAFLKKLTFKSTIILRPSLLLGNRTEFRVG
                     EKFGAIFMKTISILLFGELLKYKPIESKTVAKALFELAQQNQDGFKIYESNEIQNF"
     gene            complement(10748..11209)
                     /locus_tag="P5335_00050"
     CDS             complement(10748..11209)
                     /locus_tag="P5335_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HRZ73300.1"
                     /translation="MNNNDIFKKLRVALQLRDDQIVEILQLVDFRISKAELGAFFRNE
                     DHPNFVECGDQVLRNFLNGLVIHLRGTKENPKNPTEVIKSHKAENGVKNIEEKGKKQE
                     ERKPFVKSSSNQKAPFKKKPTSKPKFQEKKPEKKIEIVEKVKYNNGKNKKS"
     gene            complement(11343..12140)
                     /locus_tag="P5335_00055"
     CDS             complement(11343..12140)
                     /locus_tag="P5335_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRZ73301.1"
                     /translation="MKVLFSILLVFAFLIFVSEKVFGQNRYSTFEINAPQLKTTKKIW
                     VYLPLDYEKSTKKYPVIYMHDAQNLFDAKTSYAGEWKVDETSDSLKVNAIIVGIEHGN
                     DKRMEELTPFKNEKYGGGKADLYLDFIAFTLKPHIDSTYRTKSKAKNTIVFGSSLGGL
                     VSFYAVLKYPEVFGKAGIFSPSFWFSNEIYDYAKNAKKSKAKLYFLCGDSESEDMVAD
                     MERMTKIMAENRCDCLHLTKQVVVKDGRHNEKMWSEQFGKAVLWLLK"
     gene            12190..12591
                     /locus_tag="P5335_00060"
     CDS             12190..12591
                     /locus_tag="P5335_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRZ73302.1"
                     /translation="MITYKEKLSLLGDMIELSKVDGQLHERELEFIKLVAEELNVKES
                     DFEELFSLPNELLVFKSEFKRIEHFYRLALLTHCDNLHHEREHEFMYQLGLKLGLNSF
                     AIKRVLGEMDKSLNRMIDPDLLLGIFKEQHN"
     gene            12768..13202
                     /locus_tag="P5335_00065"
     CDS             12768..13202
                     /locus_tag="P5335_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010517581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRZ73303.1"
                     /translation="MTLVKTNNRFAFPSLIDEFLKPDWMGGMERFNVNVPAVNIKETD
                     TTFVVELAAPGKKKEDFNIELDENVLTISSEASTENEEKDKEGKYTRKEFSYSSFKRS
                     FTLPEIVNEYDINATYENGVLHIALPKREEALPKPKRMIEIG"
     gene            complement(13258..13695)
                     /locus_tag="P5335_00070"
     CDS             complement(13258..13695)
                     /locus_tag="P5335_00070"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRZ73304.1"
                     /translation="MITLEIHYRKLENMYHLGPINRELYKGVQLTVSHERAEIIQPIE
                     SKFFHAGQSLHGSVYFKLLDDAAYFAVQSTIQDFFILTTTFNIQLLRPVTSGTVKAVG
                     NLTFKSKNLFIADAILYDEKNREVARGSGTFIKSQHLLSEKMG"
     gene            complement(13821..14648)
                     /locus_tag="P5335_00075"
     CDS             complement(13821..14648)
                     /locus_tag="P5335_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73305.1"
                     /translation="MKNTIYIFILLLTFKAKSQEQKEHPNYKNTISLEFYQPFQNNIR
                     EFSNDDWILGYEPSKKSNYSRKAVSNAFGISYERVVKNIILRTRFGFSIREINESESY
                     QNINQEEDFTTNLSKEYNYKQIHINSLIGVAKRVKLVNSFDFDFGIEFAFVQYKKANG
                     NYKYYLTQNTISDNRFLFAQNINVIDQFGSIQSIGLGPILKPQYYFSDRLVVSAEFQV
                     YFMKTFTNDKTTRIETSYSTFEDGSFENIELSQEINYNVNQWNWTKISPLIRVGYKF"
     gene            complement(14665..16653)
                     /gene="uvrB"
                     /locus_tag="P5335_00080"
     CDS             complement(14665..16653)
                     /gene="uvrB"
                     /locus_tag="P5335_00080"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRZ73306.1"
                     /translation="MKFQVVSEYKPTGDQPQAIEKLATGIINGEKYQTLLGVTGSGKT
                     FTVANVIEKVEKPTLVLAHNKTLAAQLYSEFKQFFPENSVQYFVSYYDYYQPEAYIPV
                     TGTYIEKDLSINDELEKLRLSTTSALLSGRRDVLVVASVSCLYGIGNPVEFQKNVISI
                     DRGQIISRTKLLHRLVQSLYSRTEVEFTPGTFRIKGDTVEIFPSYADEPFRVHFFGDE
                     IEEIEAFDIKTTKVIEKYTKLNIYPANMFVTSPDVLQAAIWEIQQDLVKQVDYFKEIG
                     KHLEAKRLEERTNFDLEMIRELGYCSGIENYSRYLDGRQPGTRPFCLLDYFPDDFLMV
                     VDESHVTISQVHAMYGGDRSRKENLVEYGFRLPAAMDNRPLKFEEFEGLQNQVIYVSA
                     TPADYELQKSEGIYVEQVIRPTGLLDPIIEIRPSLNQIDDLIEEIQLRCEADERVLVT
                     TLTKRMAEELTKYLTKVSIRCRYIHSDVDTLERVEIMQDLRKGLFDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSHRSLTQTVGRAARNVNGKAIMYADKITNSMQKTIDET
                     NYRREKQIAYNTLHGLSPKALNKSLGNVLSGNSVSTQYYEDKILKAAEPESLYLTKPE
                     IEKKIRDMRKMMEKAAKELNFIDAAKFRDEIKSLQEKI"
     gene            complement(16725..17669)
                     /gene="rlmF"
                     /locus_tag="P5335_00085"
     CDS             complement(16725..17669)
                     /gene="rlmF"
                     /locus_tag="P5335_00085"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HRZ73307.1"
                     /translation="MKNQPNIEKSTLHPRNNHRSRYDFETLYLKSPELKPFVFINEHE
                     IETIDFSNPEAVKSLNKALLKTYYGILDWNLPTDYLCPPIPGRADYIHYIADLLSEVN
                     DGIIPTGNEIMGLDIGVGANCIYPILGNAIYDWSFVATDINSSAIENCVDIIEKNPHL
                     NEVISLQQQLNSRFIFKDIIQPEDKFAFTICNPPFHASKEEADKSSSRKVSNLTQQKT
                     TNPILNFGGQNNELWCDGGELSFINQMIFESVKYPKQCYWFTTLVSKKDHLKSIYKTL
                     NNVGAATIKTIEMTQGQKISRLVAWTFLSEKQRKDWKF"
     gene            complement(17723..18385)
                     /locus_tag="P5335_00090"
     CDS             complement(17723..18385)
                     /locus_tag="P5335_00090"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRZ73308.1"
                     /translation="MSSHHIVRDDQEPALIIANGAACSQELLGQLLEWSPFVVVLDSA
                     IERVLELEIKVDVLLGDFDSGFDANYYKEIQYPLEIIHTPNQDKTDLEKAFEFLIERN
                     FPAVNVVWATGKRADHTITNITSIAKFRNQLKIVLFDDHSKIHLLPQKFQKWYPKNTV
                     ISLIPIGEVHGIHSENLFYPLQNDSLTIGYRTGNSNHVTEDGLITIEHNEGDLLLMEC
                     WD"
     gene            complement(18398..19219)
                     /locus_tag="P5335_00095"
     CDS             complement(18398..19219)
                     /locus_tag="P5335_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HRZ73309.1"
                     /translation="MKLFNYISLLFLVITFIGCEKVIDVDLDTAPPKLVVEASINWDK
                     NTAGNEQFIKLTTTTDYFSSIIPNVSGATVFITNSSGTIFNFVEVTEPGIYVCNNFEP
                     VINESYTLTIVYNGETFTATEKLLATPEITRIEQNNEGGILGDEIEVRFFYDDIPNEA
                     NYYLQQIDDPYKAIPEFGVLEDRFFQNNELFGLYFSEDLKSGDEISFTLNGITQNHYN
                     YMNILLSQAGGGSGGPFSTPTSTIRGNVVNQTNFNNFALGYFRLSERAKIAYVIE"
     gene            complement(19223..21601)
                     /locus_tag="P5335_00100"
     CDS             complement(19223..21601)
                     /locus_tag="P5335_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ73310.1"
                     /translation="MLFRKSILILLFLFLSGFQAFSQEKFTLSGTITDQKNNETLIGV
                     TIIITELKTGTTTNEYGFYSISLPKGTYTLEISYIGFETITSTIVLDKNIRNNISLKE
                     SQELLKEVIVSDRKVKAEIRKPEMSVNKLTIQEIKQMPVLLGEVDIIKSLLTLPGVTN
                     AGEGQSGFNVRGGSADQNLILLDEATIYNSSHLFGLFSVFNPDAIKDLKLYKGGIPAR
                     FGGRLSSVLDIYQKEGNSKDFHMTGGIGLISSRLLAEGPIVKDKASFLVAGRGSYAHL
                     FLPLFNNDNSAYFYDLNTKLSYKLNDNNNVFISGYFGRDVFNLNKSFVNTYGNSVLNL
                     RWNHLFDEKLFSNMSFIYSDYYYGLKLDIIGFNWDSGIKNYNFKYDFKHYLSNKIKLT
                     YGNNSIYYDFNPGRIEPSNDNSSINPEQLAKKYAFESAFYVDAEHELSKKITVSYGLR
                     YSMFYRLGEQTLNTYENNQAVIFNSIFQIYESATPTGTVSYKKNETIANFDNWEPRLG
                     ISYAFNDDQSIKASYNRMTQYLHLISNTQSPTPLDVWAPSDNFLKPQLLDQFAVGYFQ
                     NFKNDDYSLEIETYYKNIQDRVDYIDGANLIANEAIERVVLSGKARAYGLEVLFRKNH
                     GQFTGWIAYTLSRTEQQIEGRTPEETGINNGQWYRTGYDKLNDISVVGNYKLDEKWRF
                     GANFSLQTGQPVTFPNGQYEYNGITVPSYSLRNENNLPTYHRLDVSATLTPTSNTGRK
                     WQGEWVFGIYNVYNRMNAASITFQQNKETASNEAIRLSIFGFIPSVTYNFKF"
     gene            complement(21723..23945)
                     /locus_tag="P5335_00105"
     CDS             complement(21723..23945)
                     /locus_tag="P5335_00105"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRZ73311.1"
                     /translation="MMSKSKIIYTITDEAPMLATHSFLPIVKAFTAPANISVETRDIS
                     LAGRIIANFPEYLKEDQCINNDLLELGQLATTPEANIIKLPNISASVPQLKEVIAELQ
                     KLGYAIPEFVENPQTEEEKIVKAKYAKVLGSAVNPVLREGNSDRRAPKAVKNYARKHP
                     HSMGAWSADSKTHVAHMNEGDFYGSEQSIFIQEAGTFKIEFEAKNGTSKLLKDNAPLK
                     AGEIIDSAVLNLNALKQFIANEIEDAKAKNVLFSVHLKATMMKVSDPIMFGAIVDVFF
                     AEVFKQYAELFKELGVNTKNGLGDVYAKIAGHPKQAEVEAALNEAMSKGPAIAMVNSE
                     KGITNLHVPSDVIVDASMPAMIRTSGQMWNAEGKPQDTKAIIPDRCYAGIYTATIDFC
                     KKHGAFDPKTMGSVPNVGLMAQAAEEYGSHDKTFQIDDYGVVKVIDGNGVVLMQQAVE
                     KGDIFRMCQVKDAPIQDWVKLAVNRARLSNTPAVFWLDENRAHDRQIILKVMTYLKNH
                     DTSGLDIRIMNPIDATTFTLERIIKGLDTISVTGNVLRDYLTDLFPILEVGTSAKMLS
                     IVPLMNGGGLFETGAGGSAPKHVEQFLEEGYLRWDSLGEFLALGVSLEHLGQSLNNSK
                     ALVLSETLDSATEKFLETDKSPARKVGQIDNRGSHFYLALYWAQALAAQNKDAALKEL
                     FTNIAKELTENEEKINAELIGYQGKKQEIGGYYRPDFKLLEKAMRPSETLNKIVEKLK
                     "
     gene            24253..26319
                     /locus_tag="P5335_00110"
     CDS             24253..26319
                     /locus_tag="P5335_00110"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRZ73312.1"
                     /translation="MRYIFLLIFSFSFAQQTSNVDFKTLHATLEPNPIDKSISGEVIY
                     KFEVLKAIDTIAIDAIKMDFTSVKINGKTINFKNSGKALKLFQGFKKGKNTLTFSYKA
                     TPKQTLYFIGEGDQLQIWTQGQGKFTSHWLPSFDDVNEKVIFNLSVSFDNQFTVLSNG
                     KLKSINDNFNDKSKIWYYQMSKPMSSYLVMMAIGKFEKQTAKTKSGTPLEFYIDEKDT
                     DKFETTYKYTTQIFDYFEQEIGLNYPWEIYRQVPVHDFLYSGMENTTSTIFSQDFVVD
                     AIGFNDKTYINVNAHELAHQWFGNLITAQSGKHHWLQEGFATYYALLAEKELFGDDHF
                     QWELYEMAERLLKASKTDTIPLLNEKASSLTFYQKGAWALHSLRESIGAENFQKAVKN
                     YLKKYGFKNVNTDDFLNEVKAVSSFDTENFKRIWLEQSGFEVNQALTLLRKNAFMKSY
                     LELVDLQTKPFAEKKTIFVNLLKTEVFYPVKEEVVFQLADVPFEEKEALLKLAMDSKN
                     LKVRQAIARIMVEIPIAFQANYETLLNDESYINKEIALGTLWNLFPEKQADYLNQSKD
                     WIGFNDKNLRILWLTLALMTKDYLNENKVNFYDELLNYSSPKYESSIRQNALTNLLFL
                     NPNDQNILRNLANATTHHKWQFTLFARNQIRELIKTERHKKYFEEMLPSLNTAEQNQL
                     RRLLEN"
     gene            complement(26316..26840)
                     /locus_tag="P5335_00115"
     CDS             complement(26316..26840)
                     /locus_tag="P5335_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73313.1"
                     /translation="MESTHIIELLSYTLPALITGGVAYYFFNLHTKNEEGRRRYLLQK
                     ELQKESLPLRLQAYERMVLFLERINPAKLLIRVAPFSEDKNAYESFLIQQIEQEFEHN
                     LTQQIYLTEDCWTVITTAKNTIIQNIRKSNMSERVDSSHKLREVILSDLLDKQSPSYI
                     AVSYLKTEVIQLMS"
     gene            26959..29283
                     /locus_tag="P5335_00120"
     CDS             26959..29283
                     /locus_tag="P5335_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRZ73314.1"
                     /translation="MINEYINQLNEAQRAPVLQKDGPMIVIAGAGSGKTRVLTMRIAY
                     LMSLGVDAFNILSLTFTNKAAREMKKRISDIVGSNEAKNLWMGTFHSVFAKILRIEAE
                     KLGYPSNFTIYDAQDSVRLIGAIIKEMQLDKDVYKPKQVFSRISSYKNSLITVKAYFN
                     NPELQEADAMSKKPRMGEIYNNYVERCFKSGAMDFDDLLLKTNELLNRFPEVLAKYQD
                     RFRYILVDEYQDTNHSQYLIVRALSDRFQNICVVGDDAQSIYAFRGANINNILNFQKD
                     YADVQTFRLEQNYRSTKNIVQAANSIIDKNKTKLEKVVWTHNQDGPKIKVNRSITDGE
                     EGRFVASTIFEEKMRNQMKNGEFAILYRTNAQSRAMEDALRKRDIPYRIYGGLSFYQR
                     KEIKDVLCYLRLVINPKDEEALVRVINYPARGIGDSTVEKLTVAANHYKRSIFEVMEN
                     IDKIDLKLNSGTKRKLEDFVMMIKSFQVINESQDAFYLADHVSKKTGLVQELKKDGTP
                     EGIARIENIEELLNGIKDFTEGQKEIDGARGALSEFLEDVALATDLDKDTGDDDRVAL
                     MTIHLAKGLEFPTVFVVGMEEDLFPSAMSLNTRSELEEERRLFYVALTRAEHQAYLSY
                     AQSRYRWGKLMDSEPSRFIEEIDDQYLEYISHTETGYRYKPMMDMDIFGDVDKSKLRL
                     AKPTNGTPPKRYGEEPSSTIRKLKPVSGNAPASTNLFDSKLVPGNIVMHERFGKGEVL
                     NLEGVGADKKAEIKFDVGGIKKLLLRFAKLDVIG"
     gene            29442..29918
                     /locus_tag="P5335_00125"
     CDS             29442..29918
                     /locus_tag="P5335_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6495 family protein"
                     /protein_id="HRZ73315.1"
                     /translation="MKYSRLTKEQFEALHQEFANFLATQSIDKKEWDEMKANKPEAAE
                     QELDVFSDLIWEGVLSQTNYLEHFSKNHIFLFHCLEEEVQSIVLKTLEPEVDFMTSKG
                     LEWMVNNLFTDAIDIHVGRKKYEGEKNAAIFDIIQQGAILSDGQLYQQINKIVNVK"
     gene            29943..31934
                     /gene="ligA"
                     /locus_tag="P5335_00130"
     CDS             29943..31934
                     /gene="ligA"
                     /locus_tag="P5335_00130"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRZ73316.1"
                     /translation="MDIQNKIQSLRDELNQHNHNYYVLDKPTISDFEFDHLLKELQEL
                     ETQHPEFFDENSPSQRVGGSITKNFNTVVHEHRMYSLDNSYSTEDLMDWENRIQKILG
                     NVELEYTCELKYDGASISITYENGKLKRAVTRGDGFQGDEVTNNIKTIRSIPLQLKGT
                     YPPKFDIRGEIILPFAGFEKMNQELIEIGETPYSNPRNTASGSLKLQDSAEVAKRPLD
                     CLLYFIVGNNLPFKTQYEGLETARKWGFKVPSQAKLAKNMHEVLDYISYWDVHRHDLT
                     YETDGVVIKVNSLQHQDELGYTAKSPRWAIAYKFKSEQVSTRLNSISYQVGRTGAITP
                     VANLEPVQLAGTIVKRASLHNADQIEKLDIRVGDIVFVEKGGEIIPKIIAVAQRGTEV
                     EPTKYITRCPECNTELERKEGEANHYCPNFYGCPPQIIGRIQHYITRKAMDIEGLGGE
                     TVALLYQNGLVKNYADLYDLKIEQILPLERMAQKSAENLINGIEKSKEVPFERVLYAL
                     GIRYVGETVAKKLAKHYKSIDSIANASLMDLILVDEIGEKIAQSVIEFFENPENRIAV
                     ERLKNVGVQFELKESNSQVSNKLEGKTFVVSGVFEKFSRDDLKKAIEDNGGKVGSSIS
                     AKTNYVIAGENMGPAKLEKANQLGIKLISENDFILLLNG"
     gene            31927..32616
                     /locus_tag="P5335_00135"
     CDS             31927..32616
                     /locus_tag="P5335_00135"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HRZ73317.1"
                     /translation="MGKLKPAIGLYFISSILVLIAVVFNLNELEQIAKPIVIPSIFFF
                     YLQHRYKRVNWWFSIAILACFVGDMILLIDPEFEILWIILCFFISYAILIKFGIDDLV
                     PQKLTFSNIGIGVLSVFFLLFVILSIIDLIEPETTERYIMFLFYGFSLLVLTVISLFN
                     FFSDASKAALYFSIMALCIVISDVFYSFYHFIEPIEMFNYINIFFQLATYYCMVSYFL
                     VRIEKPTKFNR"
     gene            32679..34151
                     /gene="guaB"
                     /locus_tag="P5335_00140"
     CDS             32679..34151
                     /gene="guaB"
                     /locus_tag="P5335_00140"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRZ73318.1"
                     /translation="MKAHTTKIIGEGLTYDDVLLIPNYSEVLPREVNIQSKFSRNITL
                     NVPIASAAMDTVTESSMAIAMAQEGGIGVLHKNMTIEQQAAKVRKVKRAESGMIIDPV
                     TLPLNATVGDAKQAMREFSIGGIPVVDENGILKGIVTNRDLRFEKQNPRSILEVMTTQ
                     NLVTAAEGTTLQEAEGILQQNKIEKLPVVDKDYKLVGLITFRDITKLTQKPIANKDRF
                     GRLRVAAAIGVTADALERATALVNAGVDAVIIDTAHGHTQGVVTVLKQVKAKFPNLDV
                     VVGNIATPEAALYLVENGADAVKVGIGPGSICTTRVIAGVGFPQFSAVLEVAAAIKGS
                     GVPVIADGGIRYTGDIPKAIAAGADCVMLGSLLAGTKESPGETIIFEGRKFKSYRGMG
                     SVEAMQEGSKDRYFQDVEDDVKKLVPEGIVGRVPYKGELNESMQQFIGGLRAGMGYCG
                     AKDVPTLQETGRFVRITASGIGESHPHNVTITKEAPNYSR"
     gene            complement(34510..34591)
                     /locus_tag="P5335_00145"
     tRNA            complement(34510..34591)
                     /locus_tag="P5335_00145"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34555..34557),aa:Tyr,seq:gta)
     gene            complement(34742..35875)
                     /locus_tag="P5335_00150"
     CDS             complement(34742..35875)
                     /locus_tag="P5335_00150"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRZ73319.1"
                     /translation="MKKIVVLLFAAAAFISCNNLGENEFVIKGTVDGIENGKLAILET
                     QDETGMGLKATDSAIIKDGKFEFKGTTTEPELRFIQVETVQGKAVFILENGEINMKIY
                     KDSVNKTEIGGTFNNDQFSKYNNEQGAIQKKIAAFRKDNMEKMQTAIQSNDTVTVNNL
                     KNGFTKLQEELNLYNSNFAEKNPKAFISVLMLENMFYQPNADIEKIKKIYTNLDKSVQ
                     ETKPGKKIGEMIANHNAAEVGKAAPEFSAPNPEGKMVSLKESLGKVTIIDFWASWCGP
                     CRKENPNVVALYAEFHDKGLNIIGVSLDKEGDAQKWKEAIAADKLTWTQVSNLQYWND
                     PIAKKYNVQSIPATYLLDATGKIVAKDLRGEELKAKVQELLGS"
     gene            complement(36076..36567)
                     /locus_tag="P5335_00155"
     CDS             complement(36076..36567)
                     /locus_tag="P5335_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRZ73320.1"
                     /translation="MQLNAKETTLTKSLLKIVKFVDGDGIILEDIVSKKEFEVRLYGI
                     DAPEINYCNKIKKDEKELQVPAALLIKLGYLSFNFLKDQVNLGDVCTLVQEQNNLVDK
                     YGRLLGYLILNDGRVLNEIMIKEGYAKPYNEVFCEMLPMYQEWNLQAKNSSKGLYSIV
                     NKF"
     gene            36657..37460
                     /locus_tag="P5335_00160"
     CDS             36657..37460
                     /locus_tag="P5335_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73321.1"
                     /translation="MAKQKGHIKYVGTLGEVRHFKIKGNDGYFAGLKGGPTAEQVKTA
                     PGFIRTRENMSEFAACANTGKSVRIGLSALMKQMTDSQFTGRLTAIMKKINIEDGSEA
                     RGQRAVLVSQQPQYLKGLDFNRNISFNGIFSAPFTLTANVDRNESTLSVPAFNPLNFL
                     NIPSGATHFRIINALSVISDFVFNPATGAYEPAEPMLNETSNIAYSAYTPVDAVTTLI
                     DVVATLPDAPTITTNVAVLGSIGIEFYQQVGVNYYLFNAGNAMKIQEVF"
     gene            37603..37773
                     /locus_tag="P5335_00165"
     CDS             37603..37773
                     /locus_tag="P5335_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73322.1"
                     /translation="MREKEQIVFLSIPQSQGSPKLSVQKLTFRVVGIDAVFFEIMPTA
                     KFQSEWKTSGII"
     gene            complement(38766..40484)
                     /locus_tag="P5335_00170"
     CDS             complement(38766..40484)
                     /locus_tag="P5335_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRZ73323.1"
                     /translation="MQLLDNKYAFIKDLGNGGFGKVFLAKEKVSNRFVAIKQLKNTNR
                     SEQEDIIHEIETISKFENPNIVTYYHHFWEDDILYLVMEYCSGGSLRDKIRNKEITAS
                     QALDWVQTLTTSLRIIHNKGIIHHDIKPDNIVFSQNGIIKIADFGVANKDIGTRAYLA
                     PETFQWDNDTIKDPRIDIYALGVTLMEILTNQNPFFGLERDGIIEKHQTADFPIEKLP
                     NWQQEIIIKAINKVPELRFQFMVEFEEALKAKAVPIIFNKNNLKAAELIEMADKALKT
                     NKWRNAYKYLELANTRYPNNVTVLQALGKYNLRLQKIDVAKKFYEQAVKLNSRLDVQK
                     DLGWIYLENKKYPIAIGLLSDHLHRNPADYEAYNLLIRCYYETNRYEPAMELAKMLME
                     TNAALPCFANNYYISYAMHHQGKEINPISILKNYENPFIEYNYSVLKEDKKSHNFKKT
                     PTLKSKLLFMDFHFNVIKENIITFIENNNQNITPTSLNKTIIKFGRESFNQNDIEVSG
                     GSSVSRRHCIIINSKDNIWLYNLESTGTYINDEKVNLKIPLIGFNKITIDDVDFTITT
                     DKNKLL"
     gene            complement(40532..42082)
                     /locus_tag="P5335_00175"
     CDS             complement(40532..42082)
                     /locus_tag="P5335_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRZ73324.1"
                     /translation="MRITEIKLNNYRAFYGEHTISLDKDGKNLMVYGENGSGKSSLFT
                     ALQNFFLSSVQKVTVEENIFVPSSKKNTASIQVTIKESAASTKTTNFELNCTNNQIIS
                     LDKTIIADANKIKGFFDYRSLLRTHIEHENEVDLFSILINGILKHSINRFSTKEIGSE
                     WKSIHYDTFEVYQSKNVQLRIKSYLKDKFNPGLKQLIQDIEKDTNVYMSYFGDSVKIK
                     LDFTEVLHHGRKNLSGNTIKLKLDFHNTHIPKHQFFLNEARLSALAISLYLASIKVNP
                     SAGALKVLVLDDLLIGLDMSNRLPLLKILKNHFIEVPENERFQTIMTTYDKVWFELVN
                     NYFGDTNWKFIEIYSKRLTDEDFEIPYIKETQGYLDKAKTYLKEKDNKASAVYIRTEF
                     ERIVKSICEKKGLQVNYKTKQKEFTTEDFWEAITNQTDIDKEIIKEIRIHRGTVMNPF
                     SHYDLEHPEFTKELEDTIDTIEKLGKINIKSLKKTTYTDLKKQIFKLELQIKKPIIGK
                     IIRSLLDK"
     gene            complement(42090..42701)
                     /locus_tag="P5335_00180"
     CDS             complement(42090..42701)
                     /locus_tag="P5335_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRZ73325.1"
                     /translation="MIKNRVIVYIDGFNLYFGMVDAGIDNCKWLNIKSLIDSYITHNQ
                     ELIEIKYFTSRITNQPSKQKRQTTYLEALESTGINIIYGLYKAKEIECNNCGHNWSIS
                     NEKMTDVNIATHLLLDAFNDRYDTAILISGDSDLVPPVKAIHHQFNKKAVSVFFPPER
                     HNITVAGAAKGSQIIGKKKIKDHQFPLNVTKKDGYVLTKPESW"
     gene            complement(42915..46733)
                     /locus_tag="P5335_00185"
     CDS             complement(42915..46733)
                     /locus_tag="P5335_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRZ73326.1"
                     /translation="MAGKLQIQESFQKVYDLEVFKREILNSVFTSEKAKLFREENYID
                     ESNQLTASEKKAIGKVVHYGNLELDDYNTLKLYEITLQPKVRIEQSKVGIQQYVRKML
                     IAGEGVLVNFINPDNLKTWRLTLVASDTVLTDKGISVKKLNPKRYTFIVGQSEKCKTI
                     SDRFADLEKASRIVLYPSENEKGAISLVNIFSVETLSKTFFDEYTLHYNRFVESIEKS
                     NYRKSVFNIDYPVKANKKEIDAANKPIRDFVKKLLGRIVFLYFVQKKGWLGASDLEYN
                     DGDPEFIFNLFKLSGANETFYSNWLSELFFNTLNEQRPNDVFKMPNNSSVKIPYLNGG
                     LFEPEDYDNKLLTLIPSLFHSDDFEDTPLTDKLIKKKDCNHRGFLDFLNAFNFTVHED
                     SPEDHTVAVDPEMLGHIFENLLEDNKDKGAFYTPKEIVHYMCQESLIEYLNTYLNPSS
                     NSKDENGVSAYEKQNANLIMHSNQYYEEDYSDIVDFVKNKNINDYILKRAKEVNKYLD
                     TVKICDPAIGSGAFPMGLLQEIFAVKELLAFATNSEWDPAKVKENIIQNSIYGVDIEK
                     GAVDIARLRFWLSLIVNEEKPKALPNLDYKIVVGNSLISRYNLNSPIDDVFETINRNR
                     KNNKLQPITLQSYKDSINDYMYVSDSKSKREYKELIKEIKDAFVSNYGNEKIKALAKI
                     RGKYENLLRVDIFGKTIGSKTEISKAKKELDAEENKRKEANEGLFYTNAIEWRFEFPN
                     LLDEDGNFIGFDIVIANPPYIGQKGNKELFEPFIKHPAFEKKMDYWYFFLNKSFELSK
                     DSAVNTFITPNYWITAQGGKKIRKHIIDNYSIVEYINFNDNAIFAAGVHTNIFTLKKN
                     EKPNQNIKTTIYQNRYNKDFLKNQHKELNFISDQNKIFNNWTGFIHFLPADVAQIINQ
                     LYVNCEKLSDNESEGEKQIGVRSGKKVTNGICNINQGLVTGKDRNVDNINNINEGVFI
                     LNEVELTSLNLNDIEKKLIKPFFKNSDISKYFVNQRPNHFFIYANKIENENELSKFHN
                     IYQHLLKNKSILAKRSINGVLESAYNKGKWWSLTTDRPNINFDSEKILCPQRSSVNTF
                     GYSNNEWYAASDVFYITSNKNTFNLKYILAILCSKLVNFWLIYMGKRKGNALELTLEP
                     LQFIPIKKITDVEQTNFIKIADYIIFLKQQEQITDINKLISIYFEQIVDGMVYELYFP
                     ELLKQHNRTIIEYLGELPVFTDSMNDNQKMEIINTVFNRLNEKNHPVRVNLFYMNSIP
                     EIKIIEGVKE"
     gene            complement(46736..50107)
                     /locus_tag="P5335_00190"
     CDS             complement(46736..50107)
                     /locus_tag="P5335_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRZ73327.1"
                     /translation="MSTKFFTNDSENTLIKKFEGFFEHNQGIQNFDALVGYLRSSGYF
                     KVREFLNKVPKIRLLVGISADKIIAQANNKGLEFFADREKAKEDFINEILIDIENANY
                     DEKTEKGIKQFFQDLIEQKIEVRTHPEKTIHAKVYIFYKDIHNHYNTERTVITGSSNL
                     TDSGIGSKVENNYEFNVELSEYADVKFAYDEFEKLWNESENILPVDAEEIVKKSYLKD
                     DFTPYEMYIKMMIEYFGNRVEYDPYNIELLLPEKYMRLKYQTDAANQGYSIMQKHNGF
                     ILADVVGLGKTIIATMIIKKFIYENGSHSKVLIVAPPAIKANWQRTAQDFLIDNHLEY
                     VTTGSLHKVIDNDYTDLPNAERYDLIVVDESHKFRNDYTEMYLALQEICKKPRVKAGE
                     NGDTRKKVILISATPLNNRPDDIENQLYLFQDRRNSTLDIPNKNLQEYFKPINEQYKK
                     LAAEPKLNIPKLKLMFNKLRNDVVEPLVIRRTRKDIENNKEYLEDLKNQKIVFPKVGD
                     PISLHYQLDEELSKLFIDTIDLIAGLDENGKPLSNALGYFRYRAIEYLVNPEDIKLYA
                     KRNQTVQSISERLSAIMKTLMVKRLESSFHAFKKSLGRLDKATQNMLDMLDKDRVFIA
                     PDLNINELIEKGLTDDEILSSIENKGGNNREFKKSAFKEEYIELLLKDKKKISDLIKR
                     WNKISVDPKMEEFLYNLKNTFFTKKINHSGKIVIFTESTETANEIKHKLEADGFEKIL
                     TIDSSNRKNADGIIRSNFDANLEESEWHYDYDIIITTEVLAEGINLHRSNVIVNYDVP
                     WNSTRLMQRIGRVNRIGTRAKQIFVYNFYPSVQGNNQIRLEQTAIRKLQAFHTAFGED
                     NKIFSLLEEVGDGALYGNKIQQEESEILKYLNELREFKKNNKEYFKKISQIPNKARVG
                     RKEDNLHFKPISFNDGDDLPDNVTGSSICYLKADNHPGVFCFVSGVGNTRILSFLDAV
                     KLFQSTPEEKRVAFHDNHFEQAKIAFQQFKSDSVQSNFHSVNKKELSPAENKAISNLN
                     YAIKFSPTPQKTETMRLLVESIKNGTFSSKGTPKKVNDFFKSFETMIVKEKEAFYEKL
                     FIEVIDDLNLNPSANNTKKEYQIINPKIVLTVSIN"
     gene            complement(50176..>51624)
                     /locus_tag="P5335_00195"
     CDS             complement(50176..>51624)
                     /locus_tag="P5335_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HRZ73328.1"
                     /translation="KNQSADFAKFADFANRKPVLQKTISKAKNDPIDDENYLLNTPII
                     PQSVYDNLPPILKQGAEAFSEPRERDTFLTGALAILSGCLPNVTGEYGGRTVYPHLFS
                     FILAPAASGKGALQSSKELADKYHEEVLKNSREQKKEYDLKMAAYKKAQRFQKKDDNT
                     QEEIPEEPPFKVVFIPANTSNAKIIQHLQQNDGTGIICETEADTLGQVFKNDWGSYSD
                     LLRKAFHSEKISISRKTNNEYFEINNPRLAVALSGTPQQVYNIIASAEDGLFSRFVFY
                     VFKTNSKWIDPSPYGSRVNLTDHFAKLSNVVYEMVLFLDSGKTKIHLTKEQWDKFNPT
                     FSEYLSQISAFVSEDAQSIVKRLGLVLYRMCMIFTSIRKFQAQEQATDIQCLDEDFET
                     ASQLIEVYLKHNILMFENLPKQEDEENGPFKKGQNKKLFFDALPNHFTRKEAVELGAT
                     FNIAERTVGSFLKSCLGNYLQQPEYGTYTKII"
BASE COUNT        16346 a         8394 c         8412 g        18372 t
ORIGIN      
        1 gtattttagt aatcttcgtc gtcgtcgtca tcctcatcag agggttcatc gtcatcatcg
       61 tcctcgtcgt cagagccgcc ggaatctttt aataaatcta cttccgtatc atcgtcagat
      121 ggagcatcgt catcttcttc atcaacatcc acttcatcga tatcatcatc taaatcaaga
      181 cctttaaccg gtgcaatagt ttcaacttct acatctaaat cttcatcttc ttcaaagttt
      241 tcaattcgtg tagctagttt tttactcact ttaaccaagt aaatagtgtc ttccgtgcga
      301 acttcaacgg cttcaattaa ttcgttttgg gcatttctaa atcgaataat atcagaatct
      361 tcatagccat caggaaattt ttccacaagc atggataaga tatcgtttgt cagtttggca
      421 taatcaacaa taattcttct cataaaaaaa tcaattataa atcaagtaag taagcaaata
      481 ttaatggagc tacaatggta gcatcagatt cgataataaa tttgggtgta tgaatgtcta
      541 atttacccca agttattttt tcgtttggaa cagcacctga gtaggagcca taacttgtgg
      601 ttgaatcgga aatttggcaa aaataactcc aaaatggaat atcatgcatt tccatatcct
      661 gatacaacat aggaactaca cagattggaa aatctcctgc aataccacct ccaatttgaa
      721 agaaaccaac tcctttacca ccggaatttt ttggatacca atcagctaac cacatcatgt
      781 attcaatgcc acttttcatt gtagtgggtt tgaattctcc tttgatgcaa taggaagcga
      841 aaatattacc cattgtactg tcttcccaac cgggaacaac aattggaagg tttttttcag
      901 cagcggctac catccaagaa tcttttgggt cgatttcgta gtattgtttt aaaactcctg
      961 agtttaacat ttggtacatg aattcatgag gaaaataacg ctctcctttt gaatcggcat
     1021 tattccaaat atcaaacaga tgcgattgta atcttctaaa agcttcttct tccggaatac
     1081 aagtgtcagt aactctgttg taatgattct ccagcaagtc ccattcttct tgtggactca
     1141 aatctcgata attaggaact ctcttatatg aattatgagc aactaagttc ataatatctt
     1201 cttctaaatt tgctccggta caagaaataa tgtgcacttt gtcttggcga atcatttctg
     1261 ccaatgattt tcctaactcg gcggtactca tcgcaccggc aagcgtaatc atcatttttc
     1321 cgttttcaag caaatgttgt tcatatcctt tagcggcatc gacaagtgct gcagaattga
     1381 aatgaagata atttttctca ataaattgac taataggtcc tttgctcatt tatattttat
     1441 attttattaa ctgttttaag ttggttcaaa gaaaaacttt ttttatgaaa agacaaatac
     1501 ttttatcaaa caaaaaatat tatttttttt catagcctaa aatggataat acttgttcgg
     1561 cactttgttg ttctgaaaat acttccgtag ccaaaatacc attttcatct ctgtcaatca
     1621 aaatatgttt gggttgcgga atcaaacagt ggtgtaatcc accaaaaccg ccaatagttt
     1681 cctgataggc acctgtatta aaaaatccga tatataatgg tttttctttg ttatatttag
     1741 gtaaataaac cgcattcatg ttttgttcgg aattgtaata atcatcgcta tcacatgtta
     1801 acccacctaa taaaactctt tcgtaggtgt cgttccatcg gttgatagcc aacatgacaa
     1861 aacgtttgtt gattgcccat gtatcaggta aagtagtgat gaaagaggaa tcaatcatat
     1921 tccatttttc acgatcattt tgttgttttt ggtacaaaac ctggtaaatt gctccaccac
     1981 tttcacccac ggtaaacgaa ccgaattcag taaaaatatt aggcacatca acttctgctt
     2041 cttcacatga tattttaatt tgattgataa tttcatcaat catatattgg taatcatatt
     2101 caaaagctaa tgaattttta attggaaatc caccaccaat atttaaactg tctaatgtag
     2161 gacattcttt ttttaatgct acataaactt ttaaacattt caacaactca ttccagtaat
     2221 aagccgtatc acgaatgccg gtattgataa aaaagtgaag cattttaagt tctacttttt
     2281 tattttcttc aatttgtttt ctgtagaaag gaagaatgtt tttataacca atccctaaac
     2341 gggaagtata aaactcaaat tttggttctt cttccgcggc aattctaatt ccaattttga
     2401 attttccatc aattttgtct tgcaacaaat ctaattcttc ataattatcg attattggaa
     2461 ttgttttgtt atgcccacca ttgattaatt tggcgatatt ttcgatgtat tgttctcttt
     2521 taaaaccatt acaaattaca taggtatttt tgttgatttt accttcagcc attaaacttt
     2581 caacaatatt aatatcaaaa gcagaggaag tttcaatatg aatattattt ttaaatgctt
     2641 cattcatgat gaaactaaag tgagaacttt ttgtacaata gcaataataa tacttggctt
     2701 cgtatttatt tttctccatg gcttttctaa accaaccttt tgctcgattg atattgctag
     2761 aaatttgagg caaataagta aactttaacg gagtaccata ttgttcaaca attttcatca
     2821 aatcgatatg atgaaattga aggttgtctt tatttaatgt aaattcttct tgtggaaagt
     2881 agaacgtttg attgattagg tcgaaatatt ttgtattcat ttaggtttca ataatttaga
     2941 ttaaaagtaa agtaaaaaac taagaatcta attttcaaac ccgaatattt gcaaaaataa
     3001 tttgcagttt ttcatgaaaa aggaaattac tatctgatat gtctaaactt aaagaatata
     3061 ttttattcaa agaattattt aaaaatcgtt ggaaagccct attaatacag taagtatcta
     3121 gaagaaacac atttttattt tgactatttt tcattaaggc aaatgtaaaa aatattatta
     3181 gcgaaataca aacctttact taaaaaaata gttaacattt ataatcttca aaagcattaa
     3241 taatccactc attttcagca tattgattaa tttttatttt tccaattccg tctaataagg
     3301 caaattgaat ttttccatat tcattttttt tgtcatgtat caataaatcc atgatgggct
     3361 gtaagtcatt ttcttcaaaa tcgataggtt caaaaatagc ttgaataccc gatttaattt
     3421 gactgtattc ttctcttgaa attaactctt tttgcattga tatataactt tctaaaatca
     3481 ttcccgcagc aattgcttca ccgtgtagaa gagtcttttt tgttgaactt tccaaaaaat
     3541 aactttcaat ggcatgaccc aaagtatgcc cgaaattcaa ggcttttcgt attccatttt
     3601 ccgttggatc ctgacttacg atagcgtttt tgattacaac agaatggtaa attaagtcat
     3661 ctaaatcttc ggttgtgaaa tcgtttaaat ttaaaaattt ctcccaatag gatttgtcaa
     3721 aaatgagtcc gtgttttaac atttctgcca atccggaacg catttcgttt tgaggtaatg
     3781 tttctaaata agttgaatct atcaatacag ctttaggcga atttataacg ccaatttgat
     3841 tttttaaatt agctaaatct actccgtttt ttcctccaat agaggcatca accattgcca
     3901 ataaagtggt tggtatatta ataaaatcga ttcctctttt aaatgttgaa gcaacaaaac
     3961 cgcctatatc cgaaattaca ccgccaccta aattaaaaag tagagatttt ctatcgccac
     4021 ctaattcggt caaagaatgc cataattgaa cgcacgtatc gatggtttta tgactttctc
     4081 ctgattcaat ttcgattatt tcaatttcta aatctgttgc tatatgagcc aaaaaatgag
     4141 ataaacaatt tgcataagta tttgaatcaa ccaaaacaaa aagtttagaa tagttttttt
     4201 cttctaaata agaatttaga aaaatatacc cattttgatt gaaattgatt gaataaccag
     4261 tggcttgtat tttttgcata ataataaaag aagttaacaa acgtatttgt tggatattat
     4321 tgtattataa ttatttgtac aaattaagtc aaaaaaatca ttaaattctc aaacactcct
     4381 tatctttgct gtattatttt caataaatga tggaaaaaat cttcgacaat acccaagttg
     4441 ctttttctct taaaagtgat tctgaattag atagagctta ttttttattc aaactcattg
     4501 acaacgagcc gttagtgcgg atcggaacag cagtgacaaa ttttgcttta aaagcacatt
     4561 tgccggtgga aggcttaatc agagcttctg tttttgatca tttttgtggc ggagtcagtg
     4621 aagatgactg tatgcccgtc atggaaaaaa tgttcactaa aaaagtttgt tcagtattag
     4681 attattctgt ggaaggtaaa gaagatgagc atcaatttga tgcggctatg aaaaaaacgc
     4741 tgaaaatcat agaatttgca aaacttaaag atgcaattcc gtttgcagtt tttaaaccaa
     4801 ccgggtttgg acgacttgat ctttatacta aagtaggaag aaaagaccca ctcaattttg
     4861 aagaacatca agaatgggat agagtcgttg ctcgttatga agccgtttgt aaattggcct
     4921 ttgaaaagga cgtttcttta ttaattgatg cagaagaaag ttggatgcaa gatgctgccg
     4981 atgaattggt aactaaaatg atgcaaaaat ataataaaga aaaggcaatc atttttaata
     5041 ctattcaatt atatagatgg gatagacttg actttcttaa aaaattacat gaacaagcaa
     5101 aacgggaagg tttttatatc ggaatgaaat tggttcgtgg ggcttatatg gaaaaagaga
     5161 atcagcgtgc ggaggaaaaa ggatataaaa ccccaatctg tgctactaaa gaagaatcag
     5221 ataaaatgta tgatgacacg ttggcttaca tgctagataa tatcaatcag atggctgttt
     5281 ttgcaggtac acataacgag ttgagttcct ataaattgat gcaactgatg gcagaaaaag
     5341 ggctgtcaaa caatgataac agaatttggt ttggtcaatt gtatggaatg agtgacaata
     5401 tcagttataa tttagcggca aatggttaca atgttgcaaa atatttacca tttggtccgg
     5461 ttcgcgatgt gatgccctac ttaattcgaa gagcagaaga aaatacttcc gttgccggtc
     5521 aaaccagtcg tgaattgatg ttaataaaaa aagaacgtga gcgtcgaaaa ttacaggacg
     5581 attaaaatat aaaaagacac aaaccttatg atttgtgtct tttttttatg aattaattta
     5641 accttaaaat ttattctcta cttcggctgc attcagcatg aaatagtgta aatcggtatt
     5701 tttgataaat ggttttaagt tttcacttcc cggaccaaac atggctaatt ctgtataatc
     5761 tgccgagtgg tccatgctaa tccaaccaac ggatgttcgt gatttttgca tttcagcaaa
     5821 agctttataa ggaagatttt tgtaattgta caaaccgcta tcctcttttt tcaatccttc
     5881 ataatagctt aaaatagttt ttgcttcctc atcatttaat gcaaaaccgt tagcaaattc
     5941 aattctttct ttcacttgag agagagtaga atttggatta aaaccattta atatccattc
     6001 gttggtgtgt ttaaaatgtt gaatactgtc aaaatggtcg tttgaagtac taccataaat
     6061 aattccggga ttggcattac catggtctga tgtgatgata attaaggtat tgctgtcttt
     6121 ttctgcaaaa tcaatagcca cttttatggc ttcatcaaag gcaacttgat catataataa
     6181 tccggcaata tcattggcat gagctgccca atctactttt cctccttcta cttgcaaaac
     6241 aaatccttct ttatgatttt tcatccggtc aatagctttt tgagtcattt cagctagcgt
     6301 tggaacagcg ttgcttaaat ccttgacatt ttctctatca atggtataag gaagtccatc
     6361 ttcataaaaa acacctaaaa taggtttgtc gtttgctgta gctaacattt cattacgggt
     6421 tttaactact tgatagccat ttgattcata agcactaaaa acatcttttt tatcacttct
     6481 taaggaacta tcaaaatatt gtagaccgcc acccatcaac acatcaaatt ttattttcaa
     6541 ataactttca gcaatttccg gttgactgtt tctgctttta ctgttcgcac aaaatccggc
     6601 aggtgttgcg tgagtaattg gaacagtagt gacacaaccg gccattttac cagctttttt
     6661 aaatttctgc catattggaa gatattcttc accatttact ccaatattta atgatccatt
     6721 tttcacacgg aaaccgcttc cccaagagga actggcagca gcagaatcag taacaataga
     6781 tgaggcagaa gccatgtcca tcaaagcacg atttactttg ttttctcgat ataaatttaa
     6841 ccaatttgaa cctttaccgg tttttctatt taaataaata tcagccatgt ttaacgttcc
     6901 ggaactcatt ccgtcactca ctaatacaat aatatttttg gttttcttcc cttttaaggt
     6961 tgtagaaata tccggatgtt gagcaatagt ttcaaatgga ttcaaaaaag cagaacctaa
     7021 cgtaaagagt gttccgtttc tgaaaaattt tcttctgtcc atgtattttt tattttaaag
     7081 aattcaaata taaaaaaaca aaggaatgaa agtgttaatt tgaaattaaa aacacattaa
     7141 ataaaaaaag ccggaaaata attccgactc tttcaaggtt gggttagtta atttataaaa
     7201 aggtgatttc aatttggcca cgaactaaat ctacaaatgt ttcccgttcg cgaatcaaat
     7261 gtccttttcc ttcataccaa agtacttcgg caggacgaaa acgtgaatta taattagagg
     7321 acatcgaaaa acaatatgct ccggcatttc taaaacacaa aacatctcct tctttaattt
     7381 ccgctattct tcggttattg gcaaacgtgt cggtttcgca aatatagcct actactgaat
     7441 aaaaacgttc tttccctttt ggattggaaa tgttttcaat atgatgatag gagccataca
     7501 acattggtct tattaaatga ttaaagccgc tatcaattcc agcaaatact gttgatgttg
     7561 tttgttttac gacatttact ttagcaagaa aaaagccggc ttcactcact aaaaattttc
     7621 cgggttcgaa tgctaaagtt aacgggcgac catattcttt ttcgaattga ttgaatcgtt
     7681 tggatagttt tttacccaat tcttccacat tagtttcaat atcgtctttt ttataaggaa
     7741 ctttaaaacc acttccaaaa tcaataaaat ctaatgtttt gaaatgtttt gctgtttcaa
     7801 ataaaatttc agcagcatag agaaacactt caatatctaa aatatcagaa ccggtgtgca
     7861 tatgaatacc attgatatgc atatgtgtat tatcaattat tcgcaataga tgtggcaatt
     7921 gatgtatgga aataccaaat ttactgtcta tatgaccaac agaaatattt gtgtttccgc
     7981 cagccatcac gtgcggatta attcgaatac aaaccggaat agacggatgt ttagttccaa
     8041 attgttctaa aattgataaa ttatcaatat taatttggac tcctaaagcc gctacttctt
     8101 caatctcatc cattgaaaca ccatttggtg taaaaatgat ttgtgcgggt ttaaatccgg
     8161 cagctaagcc taacttaact tcctgaatgg atacagtatc tagaccggca ccaattttgt
     8221 gaaacagctt taagatggat atattagaca atgcttttgc tgcataattg atttgcagcc
     8281 gttccacttt tgaaaaggca tttgctaacc gtgtatattg tgactcaatt ttggtagcgt
     8341 cataaacata aagcggactt ccaaattcat ctgctaatcc aactaattct gagctattca
     8401 ttttattttt ttagcaaagg tattgtggtg aaattgatat tacaagaaat aagaaatcaa
     8461 taacaaataa taacaatatg ttatatttgt aacaattaat aaattgattt gagttttata
     8521 ctttttccat atatagtaat ttggcatttt tgtattcaat aaaattcaaa atgtagtaac
     8581 ttttcagatt tagtttgtta tttttgttgc acttttttaa cagacaaacg catttcgatt
     8641 atgaatatac acgaatatca aggaaaagaa attttggcca gttatggcgt acgaattcaa
     8701 cgtggaatcg ttgcaaacag tccggtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnntttt ggccagttat ggcgtacgaa ttcaacgtgg aatcgttgca aacagtccgg
     8881 tagaggccgt tgcagcagcg aaacaattaa ctgcagaaac cggaaccggt tggcacgtca
     8941 ttaaagccca agttcatgcc ggtggacgcg gtaaaggcgg cggagtgaag ttggccaaaa
     9001 acttgcaaca agtggaagag ttggctgaac aaatcatcgg gatgcaatta attactcctc
     9061 aaacgccacc ggaaggcaaa aaagtacata aagttttagt agctgaggat gtttattatc
     9121 ccggggaaag tgaaacttca gaattttata tgtctgtttt attaaataga ggacgaggtc
     9181 gtaatatgat tatgtattct actgaaggtg gaatggatat tgaggaagtg gctgaacaca
     9241 caccgcattt aatttttacg gaagaaattg atccaactgt tggattacaa gcttttcaag
     9301 ctagaagaat tgcatttaat ttaggccttt ccggaactgc attcaaagaa atggttcagt
     9361 ttgttcaatc gttatataat gcctacattg gttcagatgc ttctatgttt gaaatcaatc
     9421 cggttttaaa aacatctgac aacaaaatta tggctgtaga tgccaaagta aacattgacg
     9481 ataacgcatt atacagacaa gcaaaaatcg ctgatatgcg tgatattcgt gaggaaaacc
     9541 caattgaagt ggaagcgaaa gctgccggtt tgaattatgt tgaccttgac ggtactgttg
     9601 gttgtatggt aaatggtgcc ggacttgcta tggcaacgat ggatttaatt aaatatgcag
     9661 gatatgaacc ggcaaatttt ttggatgttg gtggaactgc tgatgcaaaa agagttgaat
     9721 tagctttcag aatcatttta aaagatccga atgttaaagc aattttaatc aatatttttg
     9781 gaggaattgt acgttgtgat cgtgttgcac aaggtgttgt agatgcttat aaaaatatgg
     9841 gagacgcaat taaagtgcca atcattgtgc gtttacaagg aaccaatgca gagattgcaa
     9901 aagaattaat tgataattca ggaatgccaa ttttatcggc agtccaattt caggaagctg
     9961 cagatcaagt aaaagtagct ttaggataat ttgttactat ggttatttaa ataattagca
    10021 cttaataaaa aaaactgccc tcagttagta taactttctg agggcaattt atttttaaaa
    10081 atttggagat tatttaaaag ttttgaatct cgtttgattc atatatttta aatccgtctt
    10141 gattttgttg tgctagttca aaaagtgctt tcgcaactgt tttgctttct atgggtttgt
    10201 attttagtaa ttcaccaaat aatagaatgg aaattgtttt cataaatata gctccaaatt
    10261 tttcgcctac tctaaattca gttctgtttc ccaagagtaa tgaaggtctt agaattatgg
    10321 tgcttttaaa agttaatttt tttaaaaaag cttccatttc tcctttcgtt ttcaagtaaa
    10381 agttagttga ttgttcatta gctcccaatg aggaaataat tagaaattgg cttactttat
    10441 tttttaaggc aatttgggca aattgttttg ggtaaatcaa atctactttt ttaaaagcct
    10501 ctcgactccc tgcttttttt attgtggttc ctatcgtaca aaagaaatca tctccgacaa
    10561 ctaaattttg gtagttttct atcttatcaa aatcaattag atgttgtatt aattttggat
    10621 gtttaatgga tgtttcacgt tttacaaata caactacttt atcataaaca ggactttcta
    10681 agattaaatt taaaagttgg gcaccaacca acccggtact acctatgata agagctgttt
    10741 tcatttttta tgactttttg ttcttaccat tattgtattt tactttctcc acgatttcaa
    10801 ttttcttttc cggttttttc tcttgaaatt ttggttttga tgtcggtttt ttcttaaaag
    10861 gagctttttg attagaagac gacttcacaa agggctttct ttcctcttgt ttcttacctt
    10921 tttcttcaat attctttaca ccattttcgg ctttatgact tttaattact tctgttgggt
    10981 ttttcggatt ctcttttgtt ccacgtaaat gaatcactaa accgtttaaa aagttacgca
    11041 atacctgatc gccacattct acaaaatttg ggtggtcttc gttacggaaa aaagcaccta
    11101 attctgcttt tgaaattcta aaatctacca attgtaaaat ttccacgatt tggtcatcac
    11161 gaagttgtaa agcgacacgg agttttttaa agatatcgtt gttgttcatt ttatgagtta
    11221 ttgagttgct gagggtttaa aattttgtgc aaatgtacga agtaaatagc atttcaaact
    11281 tacatctgca taaatcccgc aattcgcaac tcgcgacgcg taacccgcaa cctgtaactt
    11341 tttcatttca ataaccacaa aacagctttt ccaaactgtt cactccacat cttttcattg
    11401 tgtcgaccgt ctttaacaac aacttgtttg gttaaatgta aacaatcaca tcgattttca
    11461 gccattattt tagtcatacg ttccatatcg gccaccatgt cttcactttc gctatcgcca
    11521 cacaaaaaat agagtttggc ttttgatttt ttggcattct tggcgtaatc gtaaatttca
    11581 ttagaaaacc aaaaggaagg ggaaaaaata cctgcttttc caaacacttc cggatatttt
    11641 aaaacagcat aaaaagatac taatccgcct aatgaactgc caaaaacaat tgtgtttttt
    11701 gccttcgatt tagttctgta agtagaatcg atatgaggtt ttaaggtgaa agcaataaaa
    11761 tctaaataca aatcagcctt tcctcctcca tatttttcat ttttaaaagg ggttaattcc
    11821 tccattcgtt tgtcattacc atgctcaatt ccaacgatta ttgcattaac ttttaaacta
    11881 tctgacgttt cgtctacttt ccattcaccg gcataggaag ttttggcatc aaacaaattc
    11941 tgagcatcat gcatataaat gacaggatat ttttttgtcg atttttcata atccaaaggg
    12001 agataaaccc aaattttttt ggttgtcttg agttgaggtg catttatttc aaaggttgaa
    12061 tatcgatttt gcccaaacac cttttcgctt acaaagatta aaaaggcaaa aacaagtagg
    12121 atagagaaaa gtactttcat tatttgaata attaaatgga aaaactaaat tagccaaaaa
    12181 tatgatttta tgattacgta taaggaaaaa ttaagtttat taggtgatat gattgaattg
    12241 tcaaaagtgg acggacaatt gcatgaacgt gaattggaat tcattaaatt agtagcagaa
    12301 gaattaaatg ttaaggaatc agattttgaa gaactatttt ctttaccaaa tgaattattg
    12361 gtttttaaat cagagtttaa aagaattgaa catttctaca gattagcttt attgacacat
    12421 tgtgataatc ttcatcacga acgcgaacat gaatttatgt accaattggg gttaaaatta
    12481 ggacttaatt cttttgccat aaaacgggtt ttaggagaaa tggataaatc actaaatcgt
    12541 atgattgacc cggatttgtt gcttggaatt tttaaagagc aacataatta gacgattttt
    12601 ttaactgtaa aaaagtcagt taacaaatta acaatcagta gttttaactg aaaaaatgac
    12661 tgtttttgta tagaagaaat gtcatcatga cggaatattc actttggtat atattttgtc
    12721 aaattataat tgtattaaaa aacgtttaat tttaaaatta taccattatg acacttgtaa
    12781 aaacaaacaa tcgttttgcg ttcccatcat taattgatga atttttaaaa cctgattgga
    12841 tgggtggaat ggaacgcttt aatgtgaatg ttcctgctgt gaacatcaaa gaaactgata
    12901 ctacttttgt agtggaattg gccgctcccg gaaagaaaaa agaagatttc aacattgaat
    12961 tagatgaaaa tgtgttgact atttcttctg aagcaagtac tgaaaatgag gaaaaagaca
    13021 aagaaggaaa atatactcgc aaagagttca gctattcttc tttcaaacgt tcttttactt
    13081 tacctgaaat tgtcaacgaa tacgatatca atgcaactta tgaaaatggt gtgcttcaca
    13141 tcgctcttcc caaacgagaa gaagctttac caaaaccaaa acgaatgatt gaaattggtt
    13201 aaatttgttt agtattgtaa tgaaaatgca ctcaaacgag tgcatttttt tttagagtta
    13261 ccccattttt tcacttagaa gatgttgact ttttatgaaa gtaccgcttc ctctagcgac
    13321 ttcccggttt ttttcgtcat ataaaattgc atcggcaatg aatagatttt ttgatttaaa
    13381 ggttagattt ccaacagctt tgacagtgcc tgatgttacc ggcctaagta attgtatgtt
    13441 aaacgttgtt gttaatataa aaaaatcttg aatagtagat tgcactgcaa aataagctgc
    13501 atcgtctaat aatttaaaat aaactgaacc atgcaacgac tgacctgcat gaaagaattt
    13561 ggattcgatg ggttggatta tttctgctcg ttcatgactg acagttagtt gaacaccttt
    13621 gtataattct cggttaatcg gacctaaatg atacatgttt tctagttttc tgtaatgtat
    13681 ttctaaagta atcatggatt tatatttttt cttgcaaact tttaatctca tcccgaaatt
    13741 ttgcagcttc aataaaatta agttcttcag tttttatttt aattttttct ataattcatt
    13801 tttaaagttt agtgtaaatt ttaaaattta taacctaccc taattaatgg tgaaattttt
    13861 gtccagttcc attgattaac attataatta atttcttgac ttaattcaat attttcaaaa
    13921 ctaccatctt caaatgttga ataagaagtt tcaattctag tagtcttatc attggtaaat
    13981 gttttcataa aatatacctg aaactcggca gataccacta atctatctga aaagtaatat
    14041 tgtggtttca aaatgggacc caaaccaatg ctttgaattg aaccaaattg atcaataaca
    14101 ttaatatttt gggcaaataa aaagcgatta tcagatattg tgttttgggt taaatagtat
    14161 ttataattac cattagcttt tttatattga acaaaggcaa attcaattcc aaaatcaaaa
    14221 tcaaaactat taactagttt aactcttttt gctacgccaa ttaatgaatt aatatgaatt
    14281 tgcttatagt tgtactcttt cgataaattt gttgtaaaat cttcctcttg atttatgttt
    14341 tgataacttt cactttcgtt gatttctcgt atcgaaaaac caaaacgagt tcttagtatt
    14401 atgtttttta ctactcgttc ataactaatt ccaaaggcat ttgagacagc ctttcgagaa
    14461 taattacttt ttttagatgg ttcataacct aaaatccagt catcattaga gaactctcga
    14521 atattatttt gaaagggttg ataaaattct aatgaaattg tatttttata gttaggatgt
    14581 tctttctgtt cttgtgattt tgctttaaaa gttaataata atataaatat gtaaatagtg
    14641 tttttcataa tcaattattt aatgttaaat tttttcttgc aaacttttaa tctcatcccg
    14701 aaactttgca gcatcaataa aattaagctc tttagccgct ttttccatca tttttcgcat
    14761 gtcgcgaatt ttcttttcaa tttcaggttt agtgagatac aaactttccg gttcggcggc
    14821 tttgagaatt ttatcttcat aatattgtgt cgagacagaa tttccactta gaacatttcc
    14881 taaactttta ttcaaagctt ttggtgataa accatgtagt gtattgtaag caatttgttt
    14941 ctcccgacga taattggttt cgtcaattgt tttttgcata ctgttggtaa ttttatccgc
    15001 atacattatg gcttttccat ttacatttcg agcagcccga ccaaccgttt gagtcaatga
    15061 gcgatggctt ctaagaaaac cttctttatc ggcatctaaa atagcaacaa gcgaaacttc
    15121 gggtaaatcc aatccttctc gtaataaatt tactccaacg agtacatcaa acaaaccttt
    15181 tcgtaaatct tgcatgattt ccacccgttc gagggtatca acatcggaat gaatgtagcg
    15241 acaacgaata gaaactttgg ttaaatattt ggttaattct tccgccattc gtttggttaa
    15301 agtagtaact aacacgcgtt catccgcttc gcaacggagt tgaatctctt cgattaaatc
    15361 gtcaatttga tttaaactag gacgtatttc gataattggg tcaagtaaac cagtgggtcg
    15421 aatgacttgt tctacataaa tcccttcact tttctgtaat tcgtaatcag caggtgttgc
    15481 agaaacgtag attacttgat tttgaagacc ttcaaactct tcaaatttca ggggacgatt
    15541 atccattgct gccggtaaac gaaatccata ttccactaag ttttcttttc gacttcggtc
    15601 accgccatac atggcatgta cttgtgaaat ggtcacgtga ctttcatcaa caaccattaa
    15661 aaaatcatcc ggaaaatagt ctaataaaca gaaaggacga gttcccggtt gacgaccatc
    15721 taaataacgg gaataatttt caattcctga gcaataacct aattcgcgaa tcatttctaa
    15781 atcgaagttt gtgcgttctt ctaatctttt ggcttctaaa tgtttgccaa tttctttaaa
    15841 ataatcaact tgcttcacta aatcttgttg tatttcccaa atagcggctt gcaacacatc
    15901 aggagaagtc acaaacatat tggcgggata gatgttcagt ttggtatatt tttcgatgac
    15961 ttttgtagtt tttatatcaa aagcttcaat ttcttcaatt tcatcgccaa aaaagtgtac
    16021 acggaatggt tcgtctgcat aactcggaaa aatctcgacg gtatctccct tgattcgaaa
    16081 tgttccgggc gtgaattcaa cttcggtacg agagtataaa ctttgcacta accgatgcaa
    16141 taattttgtc cgtgaaataa tttgaccacg gtcaattgaa attacatttt tttgaaattc
    16201 taccggattt ccaataccat ataaacacga aacagaggcc accactaaga catctcttcg
    16261 acccgaaagt agggcagaag ttgtgcttag acgaagtttc tctaattcat cattgatgga
    16321 taaatctttt tcaatatatg tacccgtgac cggaatatag gcttcaggtt gataataatc
    16381 gtaataggaa acaaagtatt gtaccgaatt ttcaggaaaa aactgtttaa attcagaata
    16441 taattgagct gctaaagttt tgttatgagc taaaacaagc gtaggttttt ctactttttc
    16501 aatcacattg gctactgtaa atgtctttcc tgaacctgta acacctaata aagtttggta
    16561 tttctcgcca ttgataattc ctgtagctaa tttttcaatt gcttgaggtt gatcgcctgt
    16621 tggtttatat tcggatacga cttgaaattt catttttgag aggctgagtt gctgtggttc
    16681 ttagttgcta agttataaaa tgtaaaattc gttttgattt ttgattaaaa cttccagtct
    16741 ttccgttgtt tttcgctcaa aaatgtccaa gctactaagc gacttatttt ttgaccttgc
    16801 gtcatttcta tggttttgat ggttgcagca cccacattgt tgagtgtttt atagatactt
    16861 tttaaatgat cttttttaga aactagcgtt gtaaaccagt aacattgttt tgggtatttt
    16921 acactttcaa aaatcatttg gttaatgaag gaaagctctc caccatcaca ccaaagttcg
    16981 ttgttttgtc cgccaaagtt caaaatagga ttcgtagttt tttgttgggt taaatttgat
    17041 actttgcgac tcgaactttt gtcagcttct tctttagagg catgaaaagg cggattacaa
    17101 atggtaaaag caaacttatc ttcaggctga atgatgtctt taaaaataaa acgcgaattc
    17161 aattgttgct gtaggctgat aacctcgttt aaatgtggat ttttttcaat gatatccaca
    17221 cagttttcaa tagcggacga attaatatcg gtggccacaa aactccaatc ataaatggca
    17281 tttcctaaaa ttgggtaaat acaattggca ccaacaccaa tatctaatcc cattatttca
    17341 tttccggttg gtataattcc atcattaact tctgataata aatcagcgat gtaatgaatg
    17401 taatctgctc ttcctggtat gggtgggcat aaataatctg taggtaaatt ccaatctaaa
    17461 atgccataat aggtttttaa aagagcttta tttaatgatt ttactgcttc aggattactg
    17521 aaatcaattg tttcaatttc gtgttcatta atgaaaacaa acggttttaa ttccggtgac
    17581 tttaggtaca atgtttcaaa atcatagcga gaacgatgat tattgcgtgg atgtaaagtt
    17641 gatttttcta tgttgggttg atttttcaaa atttcgatgg aatataaact aaagctacaa
    17701 ctttttaatt aaatataaga atttaatccc aacattccat taataataaa tcaccctcat
    17761 tatgttcaat tgtgataagc ccatcttctg tcacatggtt actatttcct gttcgatagc
    17821 cgatagttaa agaatcgttt tgtaatggat agaataaatt ttcggaatga atgccatgaa
    17881 cttccccaat tggaattaag gaaataacgg tatttttagg ataccatttt tgaaattttt
    17941 gaggtaataa atgaattttt gaatggtcgt caaacaaaac aattttcaat tgatttctga
    18001 atttagcaat tgaagtaatg tttgtgatgg tatgatcagc tcttttaccg gttgcccaaa
    18061 caacatttac tgccgggaaa ttgcgttcaa ttaaaaattc aaacgctttt tccaaatcgg
    18121 ttttatcttg gtttggagta tgaattattt ctaacggata ttgaatttct ttgtaataat
    18181 ttgcatcaaa accgctgtca aaatcaccta ataaaacatc cactttgatt tctaattcaa
    18241 gtacacgttc gatggccgaa tctaaaacaa ctacaaaggg agaccattcc aaaagttgac
    18301 ctaacagttc ttgactacaa gctgccccat tggcaataat taaagcaggt tcttgatcgt
    18361 cacgaacgat atggtgtgat gacatttatt tttttattta ttctataaca taagctattt
    18421 tagctctttc actcaaacga aaataaccta atgcaaaatt attaaaatta gtttgattga
    18481 caacattgcc tcgaatagta gaggttggtg tactaaatgg tccaccactt ccacctcctg
    18541 cttgtgacaa taaaatattc atataattat aatgattttg agttatgcca tttaaagtaa
    18601 atgatatctc atctcccgat ttcaaatctt cactaaaata aagtccgaac aattcattat
    18661 tttgaaaaaa ccgatcttct agtacaccaa attcgggaat tgctttatag gggtcatcaa
    18721 tttgttgtaa ataataatta gcttcatttg gaatgtcatc ataaaagaat cttacttcaa
    18781 tttcatcacc taaaattcca ccttcgttat tttgctcaat gcgtgtaatt tccggagttg
    18841 ctaatagttt ttctgtggca gtaaaggttt cgccattata tacaatagtt agcgtgtagc
    18901 tttcgttgat tacaggttca aaattgttac aaacatagat tccgggttca gtaacttcta
    18961 caaaattaaa aatggtacca gaactattgg ttataaatac cgtagctccg gaaacgtttg
    19021 gaatgatact tgaaaaataa tcagtagttg ttgttaattt gataaattgt tcattgccgg
    19081 ctgtattttt atcccaattt atcgatgcct ccacaactaa ttttggagga gccgtatcta
    19141 aatcgacatc aataactttt tcacatccta taaaagttat aaccaaaaat aatagcgata
    19201 tatagttaaa taatttcatg gtttagaatt taaaattata agtaacactc ggaatgaatc
    19261 caaaaataga gaggcgaata gcttcattac tagctgtttc tttgttttgt tgaaaggtta
    19321 tagatgcggc attcattcga ttgtaaacat tgtaaatacc aaaaacccat tcaccttgcc
    19381 attttctacc ggtatttgaa gttggagtca gcgtggccga aacatctaac ctatgataag
    19441 tgggaaggtt gttttcgttt cgaagactat aactgggaac cgtaattcca ttatattcat
    19501 attgtccatt tggaaaagta accggttgcc cggtttgcag agaaaaatta gcaccaaatc
    19561 gccatttttc atccaattta taatttccaa ctacagaaat atcatttaat ttgtcataac
    19621 ctgttcggta ccattgaccg ttattaatac ctgtttcttc cggagttctt ccttcaattt
    19681 gttgctctgt tctcgacaaa gtataagcta tccatcctgt aaattgacca tgattttttc
    19741 gaaacagtac ttctaaacca taagcccgag cttttccact taaaacgacc cgttcaatag
    19801 cttcattggc aattaaattg gctccatcaa tataatccac tcggtcttgt atatttttat
    19861 aataggtttc aatttctaac gaataatcat catttttaaa attttggaaa taacctacgg
    19921 caaattgatc caataattgt ggcttcaaaa agttgtcact tggagcccat acatcaagtg
    19981 gggtaggcga ttgcgtgtta gaaatcaaat gcaaatattg agtcattcta ttgtaactgg
    20041 cttttataga ttggtcatca ttaaaagcat aagaaatacc taaacggggt tcccagttgt
    20101 caaaattagc aatcgtttca ttttttttat aagaaacagt tccggttggt gttgctgact
    20161 catagatttg aaaaatagaa ttaaaaatta cagcttgatt attttcataa gtattcaacg
    20221 tttgttcacc aagtcgatag aacatactgt aacgcaaacc gtaacttacc gttatttttt
    20281 ttgataattc atgctcagca tctacataaa atgcggattc aaaagcatat tttttagcta
    20341 gttgttccgg attaatgctg gagttatcgt ttgaaggttc aattcttccc ggattaaaat
    20401 catagtagat tgaattgttt ccgtaggtca attttatttt attacttaaa taatgtttaa
    20461 aatcatattt aaaattgtag tttttgatac cactatccca attaaaacca ataatatcca
    20521 atttaagacc ataataataa tcagagtaaa tgaaagacat atttgagaat aatttttcat
    20581 caaataagtg attccatctt aagtttaaaa cagaatttcc gtatgtattg acaaaacttt
    20641 tgtttaagtt aaaaacgtct cttccaaaat aacctgagat aaagacatta ttgttgtcat
    20701 ttaatttgta acttaattta gtatttaaat cataaaaata agcagaattg tcattgttga
    20761 aaagcggtaa aaacaaatga gcatacgaac ctcttcccgc aactaaaaaa gaagctttgt
    20821 cttttacaat tggtccttca gctaataatc gacttgaaat taatccaatt ccgcccgtca
    20881 tatgaaaatc tttactgttt ccttcttttt gataaatatc taaaactgat gataaacgtc
    20941 cgccgaaacg ggcaggaatt ccacctttat acaattttaa atctttgata gcatccggat
    21001 tgaaaacaga aaagagccca aataagtggg aagaattata aatggtagct tcatctaata
    21061 atatcaaatt ttggtcagca cttccaccac gaacattaaa tccggattga ccttcgccgg
    21121 cattcgttac tccaggaagt gtgagcaatg atttaataat atcgacttct cccagcaaaa
    21181 ccggcatttg tttgatttcc tggatggtta gtttattaac actcatttca ggttttctga
    21241 tttcagcttt tacttttcgg tcagaaacaa ttacctcttt taataattcc tgcgattctt
    21301 tcagggatat attgtttcga atatttttat ctaaaacaat tgtagaagtg atagtttcaa
    21361 atccaatata acttatttct aaagtataag ttcctttcgg taatgaaatg gaataaaaac
    21421 catattcatt tgttgttgtt ccggttttta attcagtaat aataatagta acaccaatta
    21481 aggtttcatt atttttttgg tcggttatgg ttccgcttaa agtgaatttt tcctgagaaa
    21541 aggcttgaaa tcctgagagg aatagaaaaa gcagtattaa tattgatttt ctaaaaagca
    21601 tatttcatat tttttaacaa aattgattaa aattctttaa aacaggaaat atcagatgtt
    21661 aaagtagggt taattaaaaa cgaccctttc gagtcgtttt taacttttat ttttagaaag
    21721 aattatttca atttttctac tattttattc aatgtttcgc ttggtcgcat cgctttttca
    21781 agtaatttaa aatcaggtct gtaataacct ccaatttctt gttttttgcc ttgataacca
    21841 attaattcgg cattgatttt ttcctcattt tctgttagtt ccttggcaat attagtaaag
    21901 agttctttta aggctgcgtc tttattttga gcagctaaag cttgtgccca atataaagcc
    21961 aaataaaaat gggaacctct gttgtcaatt tgtcccactt ttcttgccgg tgatttatct
    22021 gtttctaaga atttctctgt tgctgaatct aacgtttctg atagcactaa tgcttttgaa
    22081 ttatttaagg attggcctaa atgttctaat gaaacgccaa gtgccaaaaa ttctcctagc
    22141 gaatcccaac gtaaataacc ttcttccagg aattgttcta catgttttgg agcagaacct
    22201 ccggcacctg tttcaaataa tccgccaccg ttcattaacg gaacgatgga aagcattttt
    22261 gctgaggttc caacttctaa aatagggaat aaatcagtta aataatcgcg taaaacattt
    22321 ccggttacag aaatagtatc taatcctttg atgattcgtt ctaaagtaaa tgtagttgcg
    22381 tcaataggat tcatgattcg aatatccaaa ccactagtat catggttttt tagatatgtc
    22441 attactttta aaatgatctg tctgtcgtga gctctgtttt catctaacca aaaaactgct
    22501 ggtgtatttg ataatctggc acggttaact gctaatttca cccaatcttg aattggagca
    22561 tcttttacct gacacattct gaaaatgtca cctttttcga cagcttgttg cattaataca
    22621 acaccatttc catcaattac tttcacaaca ccgtaatcat ctatttgaaa ggttttatcg
    22681 tgcgagccat attcttcagc ggcttgagcc attaaaccca cattaggaac gcttcccatt
    22741 gtttttgggt caaatgcacc atgtttttta caaaagtcaa tcgtagcggt ataaattccg
    22801 gcataacatc tgtccgggat tatagctttt gtatcttgtg gtttaccttc agcattccac
    22861 atttgtccgg aagtacgaat catagccggc atcgaagcat ctacaattac atcagacgga
    22921 acatgaagat tagtgattcc tttttcagaa ttgaccatcg caatagccgg tccttttgac
    22981 atcgcttcat ttaaagcagc ttccacttca gcttgtttgg gatgacctgc aatttttgca
    23041 tatacatctc ctaatccatt tttggtattt acaccaagct ctttgaatag ttcagcatat
    23101 tgtttaaaaa cttccgcaaa aaacacatct actattgcgc caaacataat tgggtcggaa
    23161 accttcatca tagttgcttt caaatgaact gaaaacaata cgtttttagc ttttgcatct
    23221 tcaatttcgt ttgcaataaa ttgttttaga gcattcaaat ttaaaacagc actatcaata
    23281 atttcacctg ctttcagtgg agcattgtct tttaaaagtt tacttgttcc attctttgct
    23341 tcaaattcaa ttttgaaagt tcctgcttct tgaataaaaa ttgattgttc actaccataa
    23401 aaatcacctt cgttcatgtg agctacatgt gttttagaat cggcactcca agcacccata
    23461 ctgtgtggat gtttacgagc ataattttta actgcttttg gtgcacgacg gtcagagttt
    23521 ccttctcgta aaacagggtt aacagcggaa cctaaaactt tggcatactt cgcttttact
    23581 attttttctt cctcagtttg tggattttca acaaattcag gaatggcata gcctaatttt
    23641 tgtaattcag caatgacctc ttttaactga gggacagaag ctgaaatgtt tggtagctta
    23701 ataatgttag cctccggagt agtggctaat tgacctaatt ctaataagtc attattgatg
    23761 cattggtctt cttttaaata ctcagggaaa ttggcaataa ttcttccggc tagtgagata
    23821 tctcgagttt caactgaaat gttagccggg gcagtaaagg cttttacaat tggtaaaaat
    23881 gaatgagtag ctagcatagg agcttcatcc gtaattgtat aaataatttt ggattttgac
    23941 atgatggtat tatttgtttg tgtttgtttg taatctttcc caaaaaatca ggattacaaa
    24001 tataagaaaa ggagcgtaaa attatcctga aagagataac gaaatcgttt gatttacata
    24061 aatgtcattc aaaagactta aaattattaa attgacaaaa aacagctcaa aaattgagaa
    24121 ttattaagtt ttaattttga aatgattgaa gcggagattg tttttattgt aacattcaat
    24181 ttgttttttt ttctaaattg ttatttaagt aaataagaaa tcgttatttc tttgtttaac
    24241 tttgtggaag ttatgcgata tatatttcta cttattttct ctttttcctt tgctcaacaa
    24301 acctcaaatg ttgattttaa aacgctacac gctactttag aacccaatcc cattgacaaa
    24361 tccatttccg gtgaagtaat ttataaattt gaagttttaa aagcgattga caccattgcc
    24421 atcgatgcta tcaaaatgga ttttacaagc gtaaaaatca atggaaaaac catcaacttt
    24481 aaaaattcag gcaaggcatt gaaattattc caaggcttta aaaaaggaaa aaataccttg
    24541 acttttagtt acaaagcaac tcccaaacaa accctttatt ttatcggaga aggcgaccaa
    24601 ttgcaaattt ggacacaagg tcaaggaaaa ttcaccagtc attggttgcc cagttttgac
    24661 gatgtgaacg aaaaagtgat ttttaatttg tcggtttctt ttgataatca atttacggtt
    24721 ttgtctaatg ggaaattaaa aagcataaat gacaatttca atgacaaaag caaaatttgg
    24781 tattatcaaa tgtccaaacc catgtcttcc tatttagtca tgatggccat cggaaaattt
    24841 gaaaaacaaa ctgccaaaac caaatcaggc acacctttag aattttatat cgacgaaaag
    24901 gataccgata aatttgaaac aacgtacaaa tacaccaccc aaatttttga ttattttgaa
    24961 caggaaatag gtttgaatta cccttgggaa atctaccgcc aagttccggt tcacgatttc
    25021 ttatattccg gaatggagaa tacaacttcc actatttttt cgcaagattt tgtggtcgat
    25081 gccatcgggt tcaatgataa aacctatatt aatgtcaacg ctcacgaatt ggctcaccaa
    25141 tggtttggca atctcatcac tgcacaatcc ggaaaacatc attggttgca ggaaggtttt
    25201 gcaacttatt atgccttgtt agctgaaaaa gaactctttg gcgatgatca ttttcaatgg
    25261 gaattgtatg aaatggccga gcgtttgcta aaagcttcta aaacagatac gattccgtta
    25321 ttgaatgaga aagccagttc actcacattt taccaaaaag gagcttgggc gttgcacagt
    25381 ttacgtgaaa gtattggtgc tgaaaacttt caaaaagcag ttaagaatta tttaaaaaaa
    25441 tacggattta aaaatgtcaa taccgatgat tttttaaatg aagtaaaagc cgtttcttca
    25501 tttgacaccg aaaattttaa acgcatttgg ttggaacaat ccggttttga agtgaatcaa
    25561 gctctgactt tacttcgtaa aaatgccttc atgaaatcgt atttggagtt ggtcgatttg
    25621 caaaccaaac cgtttgcaga aaagaaaact atattcgtga accttttaaa aacggaagta
    25681 ttttatccag tcaaagaaga agtggttttt caattagcgg atgtgccatt tgaagaaaaa
    25741 gaagctcttt taaaattggc gatggattcc aaaaatctaa aagtccggca agctattgct
    25801 cgcatcatgg ttgaaattcc aatcgctttt caagcaaatt acgaaacact tttaaatgat
    25861 gaatcttata ttaataaaga aattgctttg ggaactttat ggaatttatt tcccgaaaaa
    25921 caagcggatt atttgaatca atcgaaagat tggattggtt ttaacgataa aaatttgcgg
    25981 attctttggt tgacattagc gttgatgacg aaagactatc tgaacgaaaa caaagtgaat
    26041 ttttacgatg aattgctaaa ttacagttcg ccaaaatacg aaagctccat tcgtcaaaat
    26101 gcattgacta atttattgtt tttgaaccca aacgatcaaa atattctccg caatttagct
    26161 aatgccacaa ctcatcacaa atggcaattt acactttttg cccgtaatca aatacgtgaa
    26221 ttaatcaaaa cggaacggca taaaaagtat tttgaagaga tgcttccgag tttaaatact
    26281 gcagaacaaa atcagttgag aaggttgctg gaaaactagc tcatcaactg aatcacctcc
    26341 gtttttaaat aactcaccgc aatataactc ggcgattgtt tgtctaataa atcacttaaa
    26401 atcacctcac gtaatttgtg agaactatcc actcgttcgc tcatatttga tttgcgaatg
    26461 ttttgaatga tggtgttttt tgccgttgtt attactgtcc aacaatcttc ggttaaataa
    26521 atttgttgcg tcaaattgtg ttcaaattcc tgctcaattt gctgaattaa aaaggattca
    26581 taggcatttt tatcttctga aaaaggagcc acacgaatta ataattttgc cggattgatg
    26641 cgttctaaaa ataagaccat acgttcataa gcttgcaatc gcaagggtaa tgattctttt
    26701 tgtaattctt tttgtaacaa ataacgacgt cttccttcct cgtttttagt atgtaaatta
    26761 aaaaaatagt aagctacacc tccggtaatt agagcaggta aagtataaga taaaagttca
    26821 attatatgag tgctttccat agcattaatt tttttatttt taagaattac aaaaatagaa
    26881 ttttagttga taaatcgtaa caattttaat tgtaataaat cattagtaag cttatttttg
    26941 aactttgcta aaaatactat gataaacgaa tatatcaatc aactcaacga agcccaacgt
    27001 gcacccgtgc tccaaaaaga cggtcccatg attgtcattg ccggtgccgg ttcaggtaaa
    27061 acgcgagtgc taacgatgcg aattgcttac ttaatgagtt tgggagtaga tgcgttcaat
    27121 attttgtcgc tgacttttac caacaaagcg gctcgcgaaa tgaagaaacg tatttcggat
    27181 atcgtaggca gcaacgaagc gaaaaacctt tggatgggta cgtttcactc ggtttttgct
    27241 aaaatacttc gtatcgaagc agagaaattg ggttatccga gtaacttcac catttatgat
    27301 gcacaggata gtgttcgcct catcggagcc ataatcaaag aaatgcaact cgacaaagat
    27361 gtttataaac ccaaacaggt ttttagccgt atttcttcct ataaaaactc cttgattacg
    27421 gtgaaagcct atttcaataa tcccgaattg caagaagcag atgcaatgag caaaaagccc
    27481 cgaatgggtg aaatttacaa taactatgtg gaacgttgtt tcaaatcggg agccatggat
    27541 tttgatgatt tattattaaa aaccaatgag ttattgaatc gttttcccga ggtgttggcc
    27601 aaataccaag accgttttcg ctatattttg gtggatgagt accaagatac aaatcattct
    27661 cagtatttaa tagtccgtgc gttatccgat cggtttcaga atatttgtgt ggtaggtgat
    27721 gatgcccaaa gtatttatgc gtttcgagga gcaaacatca acaatatttt aaatttccag
    27781 aaagattatg ccgatgtgca aacctttcgt ttggaacaga attatcgttc tacgaaaaac
    27841 attgtacaag ctgctaattc cattatcgat aaaaacaaaa ccaaattaga aaaagttgtt
    27901 tggacacaca accaagatgg acccaaaata aaagtcaacc gcagcatcac cgatggggaa
    27961 gaaggtcgtt ttgtcgcttc cactatcttc gaagaaaaga tgcgaaacca aatgaaaaac
    28021 ggtgaattcg ctattttgta tcgcactaat gcacaatcac gtgcgatgga agatgcgttg
    28081 cgaaaacgcg atattccgta tcgaatttac ggcgggttat cgttctacca acgcaaagaa
    28141 atcaaagatg tattgtgtta tttgcgtttg gtaatcaacc cgaaagatga agaagcatta
    28201 gtccgtgtca tcaattatcc ggctcgtgga attggtgaca gtacagttga aaaattgacg
    28261 gtggcagcca atcattacaa acgttccatt tttgaagtga tggaaaacat cgataaaatt
    28321 gacctcaaac tcaactcggg taccaaacgg aagttggaag attttgtgat gatgatcaaa
    28381 agttttcaag tcatcaacga aagtcaggat gcgttttatt tagccgatca tgtttcaaaa
    28441 aaaacgggat tagttcaaga acttaaaaaa gatggtactc cggaaggaat tgcccgcatt
    28501 gaaaatatcg aagaattatt aaacggtatc aaagacttca ccgaaggtca gaaagaaatt
    28561 gatggagctc gtggagcttt atcggagttt ttggaagatg tggctttggc aaccgattta
    28621 gacaaagata caggcgatga cgaccgcgtg gcgttaatga ccattcactt ggcaaaaggt
    28681 ttagaatttc caaccgtttt tgtggttgga atggaagaag atttgtttcc aagtgcgatg
    28741 agtttaaaca cccgaagtga gttggaagaa gaacgccgtt tgttttatgt agccttaacc
    28801 agagccgaac accaagcgta tttgtcgtat gcacaatcgc gttaccgttg gggaaaatta
    28861 atggacagtg agccttcacg atttattgaa gaaattgacg accaatattt ggaatacatt
    28921 agtcacactg aaacgggtta tcgctacaaa ccaatgatgg acatggatat ttttggtgac
    28981 gttgataaat cgaaattgcg attagccaaa ccaaccaatg gaacgcctcc caaacgctat
    29041 ggcgaagaac cttcttccac cattcggaaa ttaaaaccgg tttcagggaa tgctccggca
    29101 agtactaatt tatttgattc caaactcgtt cctggtaaca tcgtcatgca tgaacgtttc
    29161 ggtaaaggcg aagtcctcaa cctcgaagga gtaggagccg ataaaaaagc agaaattaaa
    29221 tttgatgtgg gtgggattaa gaagttgttg ttgcggtttg cgaagctgga tgtaattggg
    29281 tagaagttgc cagttacggg ttgcgggttt tacgaatcta agtactaaac tagtaactca
    29341 catcctgatg aaatcttaac atgacctgaa ttgaaagaat taaaattaaa taataataaa
    29401 accttagtgc cttagcacct tagtacctca gtacctttaa aatgaaatac tcaagactaa
    29461 ctaaagaaca attcgaagcc ctccaccaag aattcgctaa ttttttagct acccaatcta
    29521 ttgacaaaaa agaatgggat gaaatgaaag ccaacaaacc cgaagctgcc gaacaagaac
    29581 tcgatgtatt ctccgattta atatgggaag gtgttttgag tcaaaccaac tatttggaac
    29641 atttttcaaa aaatcacatc ttcctttttc attgtttgga agaagaagta caatccattg
    29701 ttttgaaaac actagaacct gaagttgatt ttatgacatc caaagggttg gaatggatgg
    29761 tcaataattt gtttacagat gccattgaca ttcacgtagg cagaaaaaaa tatgaaggag
    29821 aaaaaaatgc tgctattttt gacatcatcc aacaaggagc gattttaagt gacggacaat
    29881 tgtaccaaca gattaataaa atagtcaacg taaagtaatt tttgaacctc agtaccttta
    29941 aaatggatat tcaaaacaaa attcaatccc ttcgtgacga actcaatcaa cacaaccaca
    30001 attattatgt gttagataaa cctactattt ccgattttga atttgaccat ttgttgaaag
    30061 agttacaaga gttggaaacc caacatcctg agttttttga tgagaattca ccaagtcaac
    30121 gagtgggtgg tagcatcacc aaaaatttca atacggttgt tcacgaacac cgcatgtatt
    30181 cgttggataa ttcctattca actgaagatt tgatggattg ggaaaatcgt attcaaaaga
    30241 tattaggaaa tgttgaacta gaatatacat gcgaattaaa atacgacgga gcttcgatta
    30301 gtattaccta cgaaaatgga aaactgaaaa gagccgttac acgtggtgat ggatttcaag
    30361 gcgatgaagt gaccaataat atcaaaacca ttcgatcgat tccgttacaa ttgaaaggaa
    30421 cttatccacc aaagtttgat attcgaggtg aaattatttt accctttgcc ggatttgaaa
    30481 aaatgaacca agagttgatt gaaattggcg aaacaccgta ttccaatccg cgaaatacgg
    30541 cttccggaag tttgaaattg caagacagtg cagaagttgc aaagcgtcca ttagattgtt
    30601 tgttgtattt tattgtgggg aataatttac cgtttaaaac ccaatatgaa ggtttagaaa
    30661 ctgctcgaaa atggggtttt aaagttccaa gtcaggcaaa actagccaag aatatgcatg
    30721 aagtattgga ttatatttct tattgggatg tacatcgcca tgatttaact tatgaaactg
    30781 atggcgtggt aatcaaagta aatagtttgc aacaccaaga tgaattgggt tacactgcca
    30841 aatcacctcg ttgggcaatc gcttataaat tcaaatcgga gcaagtctcc acccgtttga
    30901 attcgatttc ttatcaagta ggaagaacgg gagcaattac tccggtggcg aatttagaac
    30961 cggttcaatt ggcgggaacc attgtgaaac gtgcatcttt acacaatgca gatcaaattg
    31021 aaaagttgga tattcgtgtg ggcgatatcg tttttgtaga aaaaggagga gaaatcattc
    31081 ctaaaattat tgcggttgcc caaagaggaa ctgaagtgga acctacgaaa tacattaccc
    31141 gttgtccgga atgtaatact gaattagaac gaaaagaagg agaagccaac cattattgtc
    31201 cgaattttta tggttgtcca ccacaaatca taggcagaat tcaacattac atcacccgaa
    31261 aagcaatgga tattgaaggt ttgggaggtg aaacagtagc tttattgtat caaaatggct
    31321 tggtgaaaaa ttatgccgat ttatatgatt taaaaattga gcaaattctt ccgttggaaa
    31381 gaatggcaca aaaaagtgca gagaatttaa tcaacggtat tgaaaaatca aaagaagtac
    31441 cttttgaacg ggttttgtat gcattgggaa ttcgctacgt tggtgaaaca gtggccaaaa
    31501 aattagccaa gcattacaaa agtattgact ctattgcgaa tgctagtttg atggatttga
    31561 ttttagtaga tgaaatcggt gagaaaattg cacaaagtgt gattgagttt tttgaaaatc
    31621 ctgaaaatag aatagccgtt gaaaggctta agaatgttgg tgttcaattt gaacttaagg
    31681 agagcaattc tcaagtgagt aacaaattag aaggaaaaac gtttgtagtt tcaggtgttt
    31741 ttgaaaaatt ttcccgagac gatttgaaaa aagccattga agataacggt ggaaaagtag
    31801 gaagttcgat ttctgccaaa acaaattatg tgattgccgg tgaaaatatg ggaccggcaa
    31861 aattagaaaa agctaatcaa ttgggcataa aattgataag tgaaaatgat tttatacttt
    31921 tactgaatgg gtaaactaaa accggcaatt ggcttatatt ttatttccag cattttggta
    31981 ttaatagctg ttgtttttaa tttgaatgaa ttagaacaaa ttgcaaaacc aattgttata
    32041 ccttctatct ttttctttta cctccaacat cgatataaaa gagttaattg gtggttttct
    32101 atcgcaatac tggcttgttt tgtcggcgac atgatacttt tgatagatcc ggaatttgaa
    32161 atattatgga taattttgtg tttttttatt agctatgcca ttttgattaa gtttggtata
    32221 gacgatttag tacctcaaaa attaactttt tcaaatattg gtattggagt actaagtgtt
    32281 ttttttctgc tttttgtaat tctttccatt attgatttaa ttgaacctga aacaacagaa
    32341 cgatatatta tgtttctgtt ttatggtttt tcattattag ttttaacggt tatttcctta
    32401 tttaacttct tttctgatgc ttctaaagct gccttgtatt tttccattat ggcattatgt
    32461 attgtgattt cagatgtttt ttattccttt tatcatttta tagaaccgat tgaaatgttc
    32521 aattatatta atattttctt tcaattagcc acttattatt gcatggtttc ttatttctta
    32581 gttcgaattg aaaaaccaac aaaatttaac cgttaattag gtaaattgtc ttcttaaatt
    32641 ctctatattt gctcgcaatt taactacaaa ttgcaatcat gaaagcacat acaactaaaa
    32701 tcattggtga aggtttaact tacgatgatg ttctgctgat tcctaactac tcagaagtat
    32761 taccccgcga agtaaatatt cagtctaaat tttcaagaaa tattacatta aatgttccga
    32821 ttgcatctgc ggcaatggat actgtaaccg aaagttccat ggctatagca atggctcaag
    32881 aaggtggaat aggtgtttta cacaaaaaca tgactattga acaacaagcg gcaaaagttc
    32941 gtaaagtaaa acgtgcagaa tccggaatga ttatcgatcc ggtaacgttg cctttgaatg
    33001 ctactgttgg tgatgccaaa caagccatga gagaattcag tataggtgga attccggttg
    33061 tagatgaaaa tggtatttta aaaggtattg tgacgaacag agatttacga tttgaaaaac
    33121 aaaatccgcg ttcaattttg gaagtaatga ccacccaaaa tttagtaaca gcagccgaag
    33181 gaacaacatt gcaggaagca gaaggtattt tgcaacaaaa caaaattgaa aaacttccgg
    33241 tggtggataa ggattataaa ttagtaggtt taattacttt tagagatata actaaactga
    33301 ctcaaaagcc gattgccaat aaagatcgtt ttggacgact tcgggttgcc gctgcaattg
    33361 gcgtaactgc tgatgctttg gaaagagcaa cagctttggt aaatgccggt gttgatgctg
    33421 tgataattga tactgctcat ggacatacac aaggagttgt tactgttttg aaacaagtaa
    33481 aagctaagtt cccaaattta gatgtagtgg ttggaaatat tgcaactcct gaagcggcat
    33541 tgtatttagt tgaaaatgga gcagatgccg taaaagttgg tatcggaccc ggatcaattt
    33601 gtacaacacg tgttattgcc ggagttggtt ttcctcaatt ctcagctgta ttggaagtcg
    33661 ctgcagcaat taaaggcagt ggagtaccgg ttattgctga tggcggaatt cgttatacgg
    33721 gagatattcc aaaagcaatt gcagccgggg ctgattgtgt gatgttaggt tcgcttttag
    33781 cgggaacaaa agaatctccg ggtgaaacca ttatttttga aggacgtaag tttaaatcgt
    33841 atcgaggaat gggttctgtg gaagcgatgc aagaaggttc gaaagaccgt tattttcaag
    33901 atgtggaaga tgatgtgaaa aaattagttc cggaaggtat tgttggtcga gtgccttata
    33961 aaggagagtt aaacgaaagt atgcaacaat tcattggcgg attacgtgcc ggaatgggtt
    34021 attgtggtgc aaaagatgtt cctactttgc aggaaacagg aagatttgtc cgtattacag
    34081 cgagtggaat aggggaaagc catccacata atgtaacgat tacaaaagaa gctcctaatt
    34141 attcaagata aattctgttt tttaaacaaa taaaaccatt aaagtattaa gtttgattaa
    34201 gatttctaag cttaatggaa cttgatgcct tttttttcaa aagactagag tgctaagttt
    34261 aaacaggatt accttcggtg agcctgagag ggggaaacct tacccaaaac aaaaactccc
    34321 aatacttaca aaagcgaatt tatgctattt ttttatttgt ggtaaggttt cttttttttc
    34381 aaaagactag agtgctaagt ttaaacagga ttaccttcgg tgagcctgag agggggaaac
    34441 cttacccaaa acaaaaactc ccaatactta caaaagcgag cttttgacgt attaagagtt
    34501 tttgttttgt ggggagagca ggattcgaac ctgcgaagat gtaatcagcg gatttacagt
    34561 ccgccctcgt tggccgcttg agtatctccc cgctgcttac gcgattgcaa atataaaatt
    34621 gtttttacat tgtgcaaacc tttttcaagg ttaattttga taaatttata agcgtttgga
    34681 tatgagtaaa ataaaaaaat cccctgcgta ggggatttta aattttatga aaagagatgt
    34741 attaagaacc taataattct tgaactttcg cttttaattc ttcacctctt aaatcttttg
    34801 ctacaatttt accggtagca tctaataaat aggttgccgg aatagattgt acattatatt
    34861 ttttagcaat tgggtcattc caatattgta agttagaaac ttgtgtccaa gttaatttat
    34921 cggcagcaat ggcttctttc catttttgag catctccttc tttgtctagg gaaacgccaa
    34981 ttatatttaa tcctttatca tgaaattcag catatagagc aactacatta ggattttctt
    35041 ttctacaagg cccacaccat gatgcccaaa aatcaataat agtcactttt cctaacgatt
    35101 cttttaatga aaccattttt ccttccggat ttggagcaga aaattcagga gctgcttttc
    35161 caacttcggc agcattatgg ttagcaatca tttcgccaat ttttttaccc ggtttagttt
    35221 cctgaactga tttatctaga ttggtataga tttttttgat tttttcaatg tcggcatttg
    35281 gttgatagaa catattttct aacatcaaaa cactgataaa tgcttttgga tttttttcgg
    35341 caaagttact gttataaaga tttaattctt cttgaagttt tgtaaatccg ttttttaaat
    35401 tatttacggt aacggtatca ttgctttgaa tggccgtttg cattttttcc atgttgtctt
    35461 ttctgaatgc ggcaattttc ttttgaattg ctccttgctc attgttgtat ttagagaatt
    35521 ggtcgttgtt aaatgttcca ccaatttctg ttttattgac actgtctttg taaattttca
    35581 tattgatttc accgttctct aaaatgaaaa cagctttacc ttgaacggtt tctacttgaa
    35641 taaaacgcaa ttccggttcg gtcgttgtac ctttgaattc gaattttcca tctttaatta
    35701 tggcagaatc agtagctttt aaacccattc cggtttcatc ttgtgtttca agaatggcta
    35761 attttccgtt ttcaattccg tctacggttc ctttgattac gaattcgttt tctcctaaat
    35821 tattacaact tataaatgca gctgcagcaa aaagtaatac aactattttt ttcatgatta
    35881 tgtttaaatt attttgagtg caaaagtatt taaaaaaagg gatttatgcg atatgtatta
    35941 acttattttt gggttacttt ccgatacaat atttgaaggt tttagcattt acaatagttg
    36001 ccgaagattg ggcaacaaat agccatcaaa atgggctgaa catcaataaa tacaggacaa
    36061 atatagacaa aatatttaaa atttgtttac tattgaatat aatccttttg aactgttttt
    36121 agcttgtaaa ttccattctt gatacattgg gagcatttca caaaatactt cattatacgg
    36181 ctttgcatag ccttctttta tcattatttc gtttaaaact cttccatcat tcagaattaa
    36241 gtagcctaaa agtcttccgt atttatcaac taaattattt tgttcctgta ccaatgtaca
    36301 aacatcaccc aaattaactt gatcttttaa aaagttaaat gataaatagc caagtttgat
    36361 tagtaaagca gctggaactt gtaattcttt ttcgtctttt tttatcttgt tgcagtagtt
    36421 tatttctggt gcatcaattc catataaacg aacttcaaat tccttttttg atacaatatc
    36481 ttctaaaatt attccatctc catcaacaaa ttttacaatt ttgagcagac tttttgttaa
    36541 ggtggtttct ttagcgttta attgcattat attgctttat ttaagttgtt gtatttcagt
    36601 tgtttgcatt gtaaatttag taaaaattat tgtttaattg ctaaaacaca caacaaatgg
    36661 caaaacaaaa aggacatatt aaatatgtcg ggacgttagg tgaagttcgt cacttcaaaa
    36721 ttaaaggtaa tgatggttac tttgcaggtc taaaaggtgg tccaactgcg gagcaagtaa
    36781 aaactgcacc tggctttatc cgtactcgtg agaatatgtc cgagtttgca gcttgtgcca
    36841 atactggaaa atctgttaga attggtttgt ctgccttaat gaaacaaatg acagatagtc
    36901 aatttacagg aagactaact gctattatga aaaaaataaa tattgaagat ggttccgagg
    36961 caagaggtca aagagctgtt ttggtttcgc aacaacctca atatttgaaa ggtttagatt
    37021 tcaatagaaa tatttccttt aatggcattt tttctgctcc gtttacttta acggctaacg
    37081 tagacagaaa cgaaagtact ttaagtgttc cagcatttaa tcctttgaac tttttgaata
    37141 ttccatcagg agcaacacat ttcagaatta tcaatgcttt atctgtaatc tcggattttg
    37201 tttttaatcc tgcaactgga gcttatgagc cagcagaacc aatgttaaat gaaacgtcaa
    37261 atattgcata ttctgcttac actcctgtag atgcagtaac aactttaatt gatgttgttg
    37321 caactttgcc agatgctcca acgataacaa ctaatgttgc tgttttggga agtattggaa
    37381 ttgaatttta tcagcaagta ggagtgaact actatttatt caatgctgga aatgctatga
    37441 aaattcaaga agttttctaa aaaagaccat cagtttgtaa agtttactac tttaaaacca
    37501 attacccttt cgaaagagag ggttttttgt tttgcatcaa tttgtctata gtggtgactt
    37561 atataggact aatggtgcaa ataagtgcgg aatatctatc taatgagaga aaaagaacaa
    37621 atcgtttttt tatccattcc acaatcgcaa ggaagcccga aactttccgt tcaaaaatta
    37681 acattccgag tggttggaat tgacgctgtt ttttttgaaa taatgcctac cgctaaattt
    37741 caatcggagt ggaaaacgtc tggcattatt taaaaaaaaa ttgcatccta tggggtttgg
    37801 ggaaatggaa gatttcccca actgaacttt cctagtgcgc tggcagtagc gaaacaagtg
    37861 tgtagttttt tatttgctta tcggattgtt gatttatatt ttgtggttct ggcaaatgaa
    37921 aaactatact cttgtgggca aatttgattt ttgaggatgc ctttttgttc ttgcgaaatg
    37981 aggtttttct attttattaa aatgttattc ggaaatgtta tggcatcttc aaaaaacaat
    38041 gaaaactctt tcctagtgcg tcggcagaag ctcgaagtaa gggtattctt ttttatttgc
    38101 ttattgggtt gttctgattg gttttcttgg ctgcttgatg cctgcacgta aatagaaaga
    38161 taaaacttga atgttactac aattattgtt ttgaaaacgc taggtatcaa gcagacggaa
    38221 agccaatgac ttgggttttt gtggcaaata aaaaagaaaa ctcttactgg cactattggg
    38281 attgtgggta tagaaaattg ctttaaccaa aaaaacaata aacactaaaa gcaattttta
    38341 tacttacaat gtcaatatga aaatacttcc tatttctttt tatgcggttc gattgtctgc
    38401 gtctgtttgg tttcggaaga agttatgcgg aaagataaat gttgtattta acgtaaacgt
    38461 gatgcggagg tttacaattg ctagtaacgg ctggctttgc gtctgttggg gatttaagag
    38521 cagcgtactt tctgctttgt tgattagttc aaaaacaaga taaaacgtct atttaccaca
    38581 actgccccaa tagcgcaaaa ccaccgttac tagcagttgt ttctatttat tccaatattc
    38641 acacaaagta tattttattt attttgaatt aacgaaatga agcaaacaaa gaattgatgc
    38701 taggaatact tggactagga gcaatttttg tgcaggtgaa tgaggtaatg actttgtaaa
    38761 ttttattaca gtaatttatt tttatcagta gttattgtaa aatcaacatc atcaatagtt
    38821 attttattga aacctattaa tggaattttt aagtttactt tttcatcatt aatatatgtt
    38881 cctgtactct ctaaattata tagccaaata ttgtctttgc tgttaattat aatacaatgt
    38941 cttctactta ctgatgatcc tcctgaaact tcaatgtcat tttgattaaa actttctctg
    39001 ccaaatttta ttatcgtttt atttagtgat gttggagtaa tgttttgatt attgttttca
    39061 ataaatgtaa ttatattttc ttttattaca ttaaaatgaa aatccataaa taataattta
    39121 gactttaaag ttggtgtttt tttgaaatta tgactttttt tatcttcctt taaaactgaa
    39181 taattatatt caatgaatgg gttctcataa ttttttaaaa tagaaatagg attaatttct
    39241 tttccttgat ggtgcattgc atagctgata tagtaattat ttgcaaaaca aggtaatgct
    39301 gcattagttt ccataagcat tttagccaat tccattgctg gttcatatct atttgtttcg
    39361 taataacagc gaattaataa attatatgct tcataatcag cagggtttct atgcaagtgg
    39421 tctgataata aaccgatagc aattgggtat tttttatttt ctaaataaat ccaacctaag
    39481 tctttttgaa catctaatct tgaatttagt tttactgctt gttcataaaa ttttttggcg
    39541 acatcaattt tttgaagtcg taaattgtac ttgcctagcg cttgtaatac cgttacatta
    39601 tttggatatc ttgtgttggc taattctaga tatttataag cgttacgcca tttgttagtt
    39661 tttaaagctt tgtctgccat ttcaattagt tcggctgctt ttagattgtt tttattgaaa
    39721 attataggaa cagcttttgc ttttaaagcc tcttcaaatt ctaccataaa ttgaaatctt
    39781 aattctggta ctttattgat ggcttttata attatttctt gttgccaatt gggtaacttt
    39841 tcaatcggaa aatcagctgt ttggtgtttt tctataatgc catctctttc taaaccaaaa
    39901 aagggatttt ggtttgtaag gatttccatc aatgtaactc ccagtgcata aatgtcgata
    39961 cgaggatctt tgatagtgtc attatcccat tgaaaggttt caggtgctaa atatgctctt
    40021 gtgccaatat ctttatttgc tacaccaaaa tcagcaattt tgataattcc attttgagag
    40081 aaaacaatat tatctggttt tatatcgtga tgaataattc ctttattatg aataattctt
    40141 aaagaagttg ttaatgtttg tacccaatcg agtgcctgag aagcagttat ttctttattt
    40201 cttatcttat ctctaagact accaccagaa caatattcca tcactaaata taaaatatca
    40261 tcttcccaaa agtgatggta ataggttacg atattgggat tttcaaattt tgaaatggtt
    40321 tctatttcgt gaataatgtc ttcctgttct gaacgatttg tattttttaa ttgttttata
    40381 gcaacaaaac gattagacac tttttctttt gccaaaaaaa ctttaccgaa accaccattt
    40441 ccaaggtctt taataaaagc gtatttgttg tctagaagtt gcattactat ttttaaattt
    40501 taatgagttt taaatctatt caattttgtt tttatttatc gagtaatgat ctaattattt
    40561 ttccaattat tggttttttt atttgtaatt caagtttaaa aatttgtttt ttcaaatctg
    40621 tgtatgttgt tttttttaga gatttaatat ttattttacc aagtttttcg atagtgtcaa
    40681 ttgtatcttc aagttctttt gtaaattctg ggtgttctaa atcataatga ctgaaaggat
    40741 tcattacagt acctcgatga attctaattt ctttaattat ttctttatca atatcagttt
    40801 gattagttat tgcttcccaa aagtcttctg ttgtaaattc tttttgtttt gttttataat
    40861 ttacttgcaa tccttttttt tcacaaatag attttactat tctttcaaat tctgttctaa
    40921 tgtatactgc cgacgcttta ttgtcttttt ctttaagata tgtttttgct ttatcaagat
    40981 atccttgtgt ttcttttata tatggtattt caaagtcttc atccgttagt ctttttgaat
    41041 atatttcaat aaatttccaa tttgtatcac caaagtagtt gttaactaat tcaaaccaaa
    41101 ccttatcata agtagtcatt atggtttgaa atctttcgtt ttcaggaact tcaataaaat
    41161 gatttttcaa tattttaagt aatggtaagc gattactcat atcaagacca atcaataaat
    41221 catctaatac taatactttt aatgctccag ctgatgggtt tacttttata gaagctaaat
    41281 acaagcttat tgctaatgca gataatctag cttcgtttaa gaaaaattga tgtttaggga
    41341 tatgtgtatt gtgaaaatct aattttagtt taattgtatt tcctgaaagg ttttttctgc
    41401 cgtgatgaag tacttcagta aaatcaagtt tgattttaac actgtcccca aaataagaca
    41461 tatatacatt tgtgtctttt tcaatatctt gtattaattg ttttaatcct ggattgaatt
    41521 tgtcttttag ataacttttt attcttaatt gaacattttt actttgatat acttcaaagg
    41581 tatcataatg aattgatttc cattctgaac ctatttcttt tgttgaaaag cgattgatag
    41641 aatgttttag aattccattt attaatatac taaataaatc aacctcgttt tcgtgttcaa
    41701 tatgtgttct taataaactg cgataatcaa aaaaaccttt aatcttattg gcatctgcta
    41761 ttatagtttt atctaaactt ataatttgat tattagtaca gtttaattca aaattggttg
    41821 ttttggtaga agctgctgat tctttaatag taacttgaat agaagctgta ttttttttag
    41881 aagatggaac aaatatattt tcttcaactg taactttttg aacagaagat aaaaagaagt
    41941 tttgcaaagc tgtaaataac gaacttttgc cactaccatt ttcgccatat accataaggt
    42001 ttttgccatc tttgtctaat gaaatggtat gttcgccata aaaagcacga tagttgttta
    42061 acttaatttc ggttattctc ataatttact taccaacttt caggtttagt taatacatat
    42121 ccatcctttt tggtaacatt taatggaaat tgatggtctt taattttttt cttaccaata
    42181 atttgtgagc cttttgcagc acctgcaacg gtaatattgt gtctttcggg tggaaagaaa
    42241 acactaaccg cttttttatt aaactgatga tgaattgctt ttactggtgg tactaaatca
    42301 ctatcgccag aaattaatat ggcagtatca tatctatcat taaaagcatc aagaagtaaa
    42361 tgtgttgcaa tattaacatc agtcattttt tcgttggata ttgaccaatt gtgcccgcaa
    42421 ttattacatt ctatttcttt tgctttgtaa agaccataaa tgatatttat acctgtagat
    42481 tctaatgctt ctaaataagt agtttgtcgt ttttgtttac tgggttgatt ggttattcta
    42541 cttgtgaaat attttatttc aattagttct tgattgtgcg tgatataact atcaatcaat
    42601 gatttaatgt taagccattt gcaattatca atacctgcat ctaccatacc gaagtataag
    42661 ttaaacccat ctatatatac gattactcta ttttttatca tttttaacat ttttttattt
    42721 gtgaaattaa atattcactc tatatttgca cctgtacagc tccaaggata gtatccttgg
    42781 accgatgccc gacctaaccg ttgggcattt acattttata tactttcctc ttttatacct
    42841 tcaataattt ttatgtactc acgtttttat tattgttttt tttgtgttcg tgaataaaaa
    42901 ttattgaaac acttttattc ttttacacct tcaataattt ttatttctgg aatagagttc
    42961 atataaaata agtttactct tacaggatga tttttttcat tcaatctatt aaagacagtg
    43021 ttgatgattt ccattttttg attatcattc atactatctg taaatactgg taactcgcct
    43081 agatactcta tgattgtacg gttgtgttgc tttaataact ctggaaaata caattcatac
    43141 accataccat caactatttg ttcaaaataa attgaaatta atttgttgat atctgttatt
    43201 tgttcttgtt gctttaaaaa aataatataa tctgctattt ttataaaatt agtctgttct
    43261 acatcggtaa tttttttaat tggaataaat tgtaaaggtt ctaaagttag ttctaaagca
    43321 ttcccttttc ttttccccat atagattaac caaaaattta ctagcttact gcatagtatt
    43381 gctaaaatat attttaaatt aaatgtgttt ttatttgatg tgatgtaaaa cacatcagaa
    43441 gctgcatacc attcattatt cgaataacca aaagtattta cactacttct ttgaggacaa
    43501 agaatttttt cagaatcaaa atttatattt ggtctgtctg ttgttaaaga ccaccatttt
    43561 cctttattat aagctgactc tagaactccg ttaattgatc ttttagctag aattgatttg
    43621 ttttttaata aatgctgata aatattgtgg aatttagaca gttcgttttc gttctcaatt
    43681 ttgttggcat aaatgaaaaa atggttaggc ctttgattta caaaatattt agaaatgtca
    43741 gaatttttga aaaaaggttt aattaatttc ttttctatgt catttaaatt taaactagtt
    43801 aattcaactt catttaatat aaatacacct tcgtttatat tatttatatt atctacattt
    43861 ctgtccttac ctgtaactaa accttgatta atattgcaaa taccatttgt tactttttta
    43921 cctgacctaa caccaatttg tttttctcct tctgattcat tatcagataa tttttcgcaa
    43981 ttaacataaa gttgatttat aatttgagca acatcagctg gtaaaaaatg tataaatcca
    44041 gtccaattat taaatatctt gttttgatcg gagatgaaat ttaactcctt atgttggttt
    44101 tttaaaaaat ctttattgta tctattttgg tatattgtag ttttaatatt ttgatttggc
    44161 ttttcatttt ttttaagggt aaatatattg gtatgaaccc cagctgcaaa aattgcattg
    44221 tcattgaaat tgatatactc tacaatacta taattatcaa taatatgctt tcttatcttc
    44281 ttgccacctt gtgctgtaat ccaatagttt ggtgttataa atgtatttac cgctgaatct
    44341 ttagacaatt caaaagattt atttaaaaag aaataccaat agtccatctt tttttcaaaa
    44401 gctggatgtt taataaatgg ttcaaataat tctttgtttc ctttttgtcc aatatacggt
    44461 ggattagcaa tgacaatatc aaagccaata aaattaccat cttcatctaa aagattggga
    44521 aattcaaatc tccattcaat ggcattagta taaaacaatc cttcatttgc ttcttttctc
    44581 ttattttctt ctgcatctaa ttcttttttt gctttagaaa tttcagtttt tgaaccaata
    44641 gtttttccaa aaatatcaac ccttaaaaga ttttcgtact ttcctctaat tttagcaagt
    44701 gctttaattt tttcattccc atagttagaa acgaaagcat cttttatttc ttttattaat
    44761 tctttatatt ctcttttact tttagaatca gaaacataca tataatcatt aatactatcc
    44821 ttgtacgatt gtaatgtaat tggttgtaat ttgttgtttt ttctgtttct atttattgtt
    44881 tcaaaaacgt cgtctattgg gctgtttaaa ttgtatctac ttattaatga attacccaca
    44941 acgattttgt aatcgaggtt tggtaacgct ttcggttttt cttcgtttac aattaggctt
    45001 aaccaaaaac gcagccttgc aatatccaca gcaccttttt ctatatcaac accataaatg
    45061 ctgttttgaa tgatattttc ttttactttg gctggatccc attcgctatt ggttgcaaat
    45121 gcgagtaatt cttttacagc aaaaatctct tgtaataatc ccattgggaa agctcctgaa
    45181 ccaatggctg gatcgcatat ttttacggta tcaagatatt tatttacctc tttagcacgc
    45241 ttgagaatat aatcgttaat gtttttattt tttacaaaat ctacaatatc tgaataatct
    45301 tcctcataat actgattaga gtgcattatt aaatttgcat tttgtttttc ataggctgaa
    45361 acaccgtttt cgtcttttga attggaactc ggatttagat atgtatttaa atactcaatt
    45421 aaactttctt gacacatata atgtacaatc tcttttggtg tgtagaaagc tcctttgtct
    45481 ttattatctt ctagtaagtt ctcaaaaata tgtcctaaca tttctgggtc aactgctaca
    45541 gtatggtctt ctggactgtc ttcgtgaaca gtgaaattga atgcgtttaa aaagtcaaga
    45601 aaacctctgt gattgcaatc ttttttctta atcagtttat ctgttagtgg agtatcctca
    45661 aaatcatcag agtgaaataa tgaaggtata agtgttagta atttgttatc gtagtcttct
    45721 ggttcaaata gaccaccatt taaataaggt atttttacag aagagttatt tggcatttta
    45781 aaaacatcat ttggtctttg ttcatttaat gtattaaaaa agagttctga taaccagttt
    45841 gaataaaaag tttcgtttgc accacttaat ttaaataaat taaaaataaa ttcagggtct
    45901 ccatcattat attctaaatc agacgcacct aaccaacctt tcttttgaac aaagtataag
    45961 aatactatac gacctaataa tttttttacg aaatcacgaa tgggcttatt agcagcatca
    46021 atttcttttt tattagcttt tacaggataa tcaatattga aaactgattt tctgtaatta
    46081 gatttttcaa tactctctac aaatctgttg taatgcaacg tatactcatc aaaaaacgtt
    46141 ttacttaacg tttcaaccga aaatatattt actaaactaa tagcaccttt ttcgttttct
    46201 gatggatata aaacaattcg tgatgctttt tctaaatctg caaaacgatc ggaaatggtt
    46261 ttacattttt ctgattgacc aacaataaaa gtatagcgtt taggatttag tttttttaca
    46321 ctaattcctt tatctgtcaa aacggtatcg ctggcaacca aagtaagtct ccaagtttta
    46381 aggttatctg gattgatgaa atttactaat acaccttcgc ctgctatcag cattttacgc
    46441 acatattgct ggatacctac tttactttgt tcgattcgaa ctttaggttg taaggttatt
    46501 tcgtaaagct ttaatgtatt ataatcgtct aactctaaat taccataatg aacgacttta
    46561 cctattgctt tcttttcgga tgcagttaat tgattgcttt cgtcaatata attttcttct
    46621 ctaaaaagtt ttgctttttc tgacgtaaaa acagaattta gaatttctct tttaaagact
    46681 tctaggtcgt atactttttg aaatgattct tgtatttgta gtttaccagc catacttaat
    46741 taatactaac ggttaaaaca atttttggat tgatgatttg atattctttt ttggtattgt
    46801 tagcacttgg atttaggttc aaatcgtcta taacttcaat aaataatttt tcgtaaaatg
    46861 cctctttttc ttttacaatc atagtttcaa aagatttgaa aaaatcattt actttttttg
    46921 gtgtgccttt gcttgaaaat gttccattct taatactctc taccaataaa cgcatcgttt
    46981 ctgttttttg aggcgttggt gaaaacttta tagcataatt tagattagaa attgctttgt
    47041 tttcagctgg actcaattct tttttattta ctgaatgaaa attactttgc accgaatcgc
    47101 ttttgaattg ttgaaaagca attttggctt gttcaaagtg attgtcgtga aaagccactc
    47161 ttttttcttc aggagttgat tgaaacaatt taactgcatc taaaaagctc aaaatccttg
    47221 tgttgccaac accactaaca aaacaaaaaa caccaggatg gttgtctgct tttaaataac
    47281 aaattgatga accagtaacg ttatctggta aatcatctcc atcattaaag gaaataggtt
    47341 taaaatgaag attgtcttct tttctcccaa ctcttgcctt atttggtatt tgactaattt
    47401 tcttgaaata ttctttattg ttcttcttga attctcttaa ttcattgaga tattttaata
    47461 tttcactttc ttcctgctgt attttatttc catataaagc accatcgcca acttcttcta
    47521 acaaagaaaa tattttattg tcttcgccaa aagcagtatg aaatgcttgt agtttacgaa
    47581 tggccgtttg ttctaatcta atttgattat ttccttgaac actcggataa aaattatata
    47641 cgaagatttg ttttgcacgc gtaccaatac gattgacacg accaattctt tgcattaatc
    47701 ttgtactgtt ccaaggaaca tcataattta caattacatt actacggtgc aagtttatac
    47761 cttctgctaa aacttctgtt gtaataataa tatcataatc ataatgccat tccgattctt
    47821 ctaaattagc atcaaaattg gaacgaataa ttccatcagc gtttttccta ttagaactat
    47881 caatggttag tattttttcg aaaccatcag cttctaattt gtgtttaatt tcattggctg
    47941 tttccgtact ttcagtaaag attacaattt taccactatg gtttattttt tttgtaaaga
    48001 aagtgttttt gagattgtat aaaaactctt ccattttagg gtcaacagaa attttattcc
    48061 atcgtttaat taaatcagaa attttctttt tgtcttttag aagtagttca atgtattctt
    48121 ctttaaaagc tgatttttta aactctctat tattacctcc tttattttca atagatgata
    48181 gtatttcatc atcagttaaa cctttttcaa taagttcgtt tatgtttaaa tcaggagcaa
    48241 taaaaactct gtccttatct aacatatcta gcatattttg agttgcctta tctaatcgac
    48301 ccaacgattt tttaaaagcg tgaaaactgc tttctaatcg tttaaccata agagttttca
    48361 ttattgcaga taatctttca gaaatagatt gtacggtttg atttcgttta gcatacagtt
    48421 taatatcttc tggatttacc agatactcaa tagctctgta tctaaaatac cctaatgcat
    48481 ttgataaagg ttttccattt tcatctaatc ctgcaattaa atcaattgta tcaatgaata
    48541 atttagaaag ttcttcatct agttgatagt gtaaagaaat cgggtcacca acttttggga
    48601 aaactatttt ttgatttttt aaatcttcta aatattcttt attattttca atgtcttttc
    48661 ttgttcttct gataacaagt ggttcaacta catcattacg aagtttatta aacattagct
    48721 ttagttttgg tatatttagt tttggttcgg cagctaactt tttatattgc tcattaatag
    48781 gtttaaaata ttcttgtaaa tttttatttg gtatatctaa tgttgaattt cttctgtctt
    48841 ggaataagta cagttgattt tcaatatcat caggtctatt atttaatgga gttgctgaaa
    48901 ttaatattac tttttttcta gtatctccat tttcacctgc tttaactcgt ggttttttac
    48961 agatttcttg taatgccaaa tacatttctg tgtaatcatt tctaaattta tgagattcgt
    49021 caactacaat taaatcataa cgttcagcat taggcaaatc ggtgtaatca ttatcaataa
    49081 ccttgtgtaa gctacctgtt gtaacatatt ctaaatgatt atcaattaag aaatcttgag
    49141 ctgttctttg ccagttagct ttaattgctg gtggagcaac aattaaaact ttagaatgac
    49201 taccattttc ataaatgaat tttttaataa tcattgtagc aataatagtt ttacctagtc
    49261 caacaacatc ggctaaaata aaaccattgt gtttttgcat aatagagtaa ccttgattag
    49321 cagcatcggt ttgatacttt aatctcatat acttttctgg aagtaataat tcaatattgt
    49381 agggatcata ttctacacga ttaccaaagt attcaatcat cattttgatg tacatttcat
    49441 aaggtgtaaa atcatctttt aaatatgatt tttttactat ttcttcagca tcaactggaa
    49501 ggatattttc agattcattc caaagttttt caaactcatc ataagcaaat ttcacatctg
    49561 catattcact tagctcaaca ttaaattcgt aattattttc tactttagaa cctattccag
    49621 aatctgttaa attgctagag cctgtaatta ctgttctttc agtattgtaa tgattgtgaa
    49681 tgtctttgta aaatatataa actttagcgt gaatagtctt ttcgggatga gttctaactt
    49741 caattttctg ctcaattaaa tcttgaaaaa attgctttat tcctttttcg gttttttcat
    49801 catagttagc attctcaatg tcaattagaa tttcattaat aaaatcctct tttgcttttt
    49861 ctctatcagc aaaaaattct aatcctttgt tatttgcttg agcaattatt ttatcagcac
    49921 taatacctac caataatctt attttaggaa ctttgtttaa aaattctcta actttaaaat
    49981 aacctgacga ccgtaaatac cctactaaag catcaaaatt ttgtatgcct tgattgtgtt
    50041 caaagaaccc ttcaaacttc ttaataagag tattttcgct atcgtttgta aaaaattttg
    50101 ttgacattta tataaaaaac tgttaatctc caaaaatagt aaaaattaac ttcttttttt
    50161 tgtttttgtt tgactttaga tgatttttgt atatgtccca tattcaggtt gttgtaagta
    50221 attgcctaaa caagatttta aaaaggaacc aactgttctt tctgctatat tgaaggtagc
    50281 acctaattca actgcttctt ttcgtgtgaa gtgatttggt aaggcatcaa agaatagttt
    50341 tttgttttgt ccttttttga aaggtccatt ttcttcgtct tcctgttttg gtaagttctc
    50401 gaacattaga atgttgtgtt ttaaatagac ttcaatcagt tgtgatgctg tttcaaaatc
    50461 ttcgtctaaa cattgaatgt ctgttgcttg ttcttgtgct tggaatttac gaatgcttgt
    50521 aaaaatcata cacattctat acaaaactaa acctaatctt tttacaatac tttgagcatc
    50581 ttctgaaaca aacgcactga tttgacttaa atactcgctg aatgttgggt tgaatttgtc
    50641 ccattgttct ttggttaaat gtatttttgt tttaccacta tcgagaaaca aaaccatttc
    50701 atatacaacg tttgataact tggcaaaatg gtcggttaaa ttcactcttg aaccatacgg
    50761 agaagggtca atccatttgc tgttggtttt gaaaacataa aaaacaaaac gactaaataa
    50821 cccatcttcg gcagatgcaa ttatgttata cacttgttga ggtgttcccg aaagtgcaac
    50881 agctaaacgt ggattgttaa tttcgaaata ctcattattg gtttttcttg aaatggatat
    50941 tttttcgcta tggaatgctt ttcgtaacaa atcagaataa gaaccccaat cgtttttgaa
    51001 aacttgtcct agcgtgtcgg cttcggtttc gcatataata cctgtaccgt cattttgttg
    51061 taagtgttgg ataatttttg cattactggt attggccgga atgaatacta ctttgaatgg
    51121 cggttcttct ggaatttctt cttgtgtatt gtcgtctttc ttttgaaaac gttgcgcttt
    51181 tttgtaagct gccattttta ggtcgtattc ttttttttgc tctcttgaat ttttaaggac
    51241 ttcttcgtgg tatttatctg ctaattcttt agaagattgt aacgcacctt ttcctgatgc
    51301 agcaggagca agaatgaaag aaaataaatg aggatatacg gttctgccac catattcacc
    51361 agtaacattt ggtaaacaac ccgataaaat tgctaaagca cctgttagaa acgtatctct
    51421 ttctcttggt tcggagaatg cttctgcgcc ttgtttaaga ataggaggca agttgtcgta
    51481 aaccgattgc ggaattatag gtgtgtttag taaatagttt tcgtcgtcta ttgggtcatt
    51541 ttttgctttg ctaattgttt tttgtagtac aggtttgcgg ttggcaaagt cggcaaactt
    51601 ggcaaaatct gccgactgat tttt
//
LOCUS       DAOTCW010000002        45895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_380_length_45895_cov_43.499127, whole genome shotgun sequence.
ACCESSION   DAOTCW010000002 DAOTCW010000000
VERSION     DAOTCW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 45895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45895
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_380_length_45895_cov_43.499127"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="P5335_00200"
     CDS             complement(<1..872)
                     /locus_tag="P5335_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73329.1"
                     /translation="MKKLLFTILMMLNVSFVFAQFDNISIIGQFTDWSVDVPMNTSDG
                     IIYTVTSQNFPVSGGAKFRKDNDWAVNWGAATFPSGVGVQNGADIPVPMGTYDVVFNK
                     ETGDYIFTQVESVFDNIGMIGGFNEWTESVALTTFDGILYTKDDYQFIANEVKFRKDN
                     SWEVNWGGTTFPSGEAVPGGSNIPLTSGFYNVTFNYSGLNYTFIQVPVSIIGDGAQGW
                     ETDIPLQSLDGGVTFTLENITLVDGFVKFRTNASWATNWGSTDFPAGSGTQNGSNIPT
                     VAGTYNVTFNRLTG"
     gene            complement(1076..1849)
                     /gene="mazG"
                     /locus_tag="P5335_00205"
     CDS             complement(1076..1849)
                     /gene="mazG"
                     /locus_tag="P5335_00205"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRZ73330.1"
                     /translation="MKSRTQQLEAFGRLLDIMDDLREKCPWDKKQTLESLRHLTIEET
                     YELGDAILDNDLTEIKKELGDLLLHIVFYAKIGSETDAFDIADVANSICDKLIDRHPH
                     IYGDVVVANEEEVKQNWEKLKLKEGKKSVLEGVPKSLPALVKASRIQDKVKGVGFDWE
                     EPHQVWDKVQEELSELQEEVISGNQDKIEAEFGDVLFSMINYARFLKVNPEDALERTN
                     KKFIKRFMYLENRASELGRPLVDMTLAEMDVFWEEAKKL"
     gene            complement(1923..2345)
                     /locus_tag="P5335_00210"
     CDS             complement(1923..2345)
                     /locus_tag="P5335_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HRZ73331.1"
                     /translation="MSLQKILAISGKPGLFELKIQTRTGFVAESFLDGKKITVGMRSN
                     VSLLSEIAVYTYDEEIKLAEVFKAIATKENDGPSISHKEDNGVLSSYFREVLPEYDEE
                     RVYASDIKKIINWYNMLQPKGLVTMEALSQEVKEETAE"
     gene            complement(2373..2960)
                     /gene="def"
                     /locus_tag="P5335_00215"
     CDS             complement(2373..2960)
                     /gene="def"
                     /locus_tag="P5335_00215"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRZ73332.1"
                     /translation="MILPIIGYGDPVLRKVGVEISPDYPNLDILIENMYATMYNAYGV
                     GLAAPQIGLSIRMFIIDTEPFSENDDLKTDEKNQLKSFKKTFINAKIIKEEGNEWAFN
                     EGCLSIPDVREDVFRKEKITIEYCEEDFVRKTDVFEGLIARVIQHEYDHIEGILFTDK
                     ISSLKKTLIKKRLQNIMEGKIRADYKMKFFNKKGR"
     gene            3158..3562
                     /locus_tag="P5335_00220"
     CDS             3158..3562
                     /locus_tag="P5335_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73333.1"
                     /translation="MKNYVYLIIFMFSINTFGQVTEENDQAIIEKALYSKQEKEDMLA
                     KYKTDVDIIGMTPEVKELYMNLITSNFQKMIILNKDRNNTQKELKQRLKVVLEDQRVE
                     LKKLLTYEQIKRHQQIYKPIIESIRFRIDSYK"
     gene            complement(3566..3982)
                     /gene="ruvX"
                     /locus_tag="P5335_00225"
     CDS             complement(3566..3982)
                     /gene="ruvX"
                     /locus_tag="P5335_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRZ73334.1"
                     /translation="MPRIIAIDYGLKRTGIAVTDELQIIASGLTTVPSDSVINFLKEY
                     FSKEKVIEVLIGEPKQMNGLPSESTPIIEKFVSDFKTQFPSMKVIRVDERFTSKMAFQ
                     TMIDSGLSKKQRQNKGLVDEIAATILLQDYLTRKMI"
     gene            complement(4042..4950)
                     /locus_tag="P5335_00230"
     CDS             complement(4042..4950)
                     /locus_tag="P5335_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HRZ73335.1"
                     /translation="MKITSITCIAILFFSVGCTKKTKTEATNEILNDSTTIIVIDEHN
                     AKNSLDYIGMYKGIVPCADCEGIETILNLRDESNFVLTTHYLGKKDSKINELKGTYTW
                     NKEGNTVVLSGIENGPSQYFVSENYLTQLDLEGNKITGDLASKYVLEKQNETAVEIAP
                     PSPPTKSNFNQSPTSTSVQKQVGKEVYAVVGTKWRLVELNGRAIKNINQSTKDLFLQL
                     NKENRYSAFAGCNSMMGQYELKEEIFRIKFSKGASTMMACPDMETEQEFAKMLETVDN
                     YSLNGNQMTLNKARMAPLARFEAIKK"
     gene            5069..5884
                     /locus_tag="P5335_00235"
     CDS             5069..5884
                     /locus_tag="P5335_00235"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRZ73336.1"
                     /translation="MTELKNTIEKAWNNRALLQEEATITTIRKVIDLLDNGKLRVAEP
                     TTEGWQVNEWVKKAVVMYFPIQKMETWEAGIFEYHDKMLLKHNYAEKGIRVVPPAVAR
                     YGAYISKGVILMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKDVHLSGGVGIGGVLEP
                     LQAAPVIIEDGAFIGSRCIVVEGIRVEKEAVLGANVVLTGSTKIIDVTGSEPIEYKGY
                     VPARSVVIPGSYTKKFAAGDYQVPCALIIGQRKPSTDLKTSLNDALREYNVAV"
     gene            5916..7778
                     /locus_tag="P5335_00240"
     CDS             5916..7778
                     /locus_tag="P5335_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRZ73337.1"
                     /translation="MYKDLIKLLLKRLLIVAFLYQVCRILFYAFNRNAFDKLDLFTFL
                     GGLQFDLSAIGYINLIFILGHLIPGNFKYKPIYQKGLKISFYSVNLLFIATNFVDFEY
                     YKFTGRRSSFGLITATGMENEIGVLLLSFLKEFWYLPLLLIAIGILFWKSIGNFKSGN
                     SNSTSKRHHLKEFVILIIALGLGLLMGRGGFRKKPLKVVDAISYSGTGNSALILNTPF
                     TILKTIGKKETLESPKFYDQTTLDRIFNPVVKTNPSGESTKKNVVIIILESFGNENIR
                     IGYTPFFDSLATHSYYFKNGFANGKLSIDAVPSTLSSIPSLMNHSLITSNYSLNEIYG
                     LPKILKDNGYATSFFHGAFNGSQNFDQYCKVAGFDNYFGKNEYEGPESFDGTWGVFDE
                     EFLQFFCEKLTSFKQPFFSSVFTISSHNPYTIPKKYNGKFPKGKTKILESIAYSDYAL
                     KQFFETAKKQEWYNNTIFVLTADHTSSDPILAKHKTNVGKFSIPILFFDPSNPELKGE
                     IEKNIQQIDIMPSLIDYLNIETKMVTYGKSFKSKKNFVVYFLDNIYHYINDDYYMAFD
                     GKKAIGLYNFKTDELLKNNLLKSNSVIADQMEKFIKAYVQSFNERVINNKLHVH"
     gene            7788..8855
                     /locus_tag="P5335_00245"
     CDS             7788..8855
                     /locus_tag="P5335_00245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRZ73338.1"
                     /translation="MKKILVIQQKMIGDVLLSSILCNSLRKAYPTAQIDYLVHENTVP
                     VLENNPNIDTIIRFTEKHKSSRRAFLKLGKKIRAEKYDILIDAYSKLESWLLSAMSGA
                     KQKISYKKIGRNFIYDINVPYEDAPKTSLGLAIERRLSLLKPLSIEKYINPIPKLFLT
                     NAEMQTAKNLFKKFGINESKKTVMISLLGSEEKKTYPLPFMAKIIDFIGQNYDVTILF
                     NYFPKQIKEAKIILNLCSEKTKSKIYFDFLGQDLRSFIAIMNECDAIIGNDGGAINMA
                     KALNKPSFIIFSPWIEKKMWATFEDGINHSSVHLEDYQPNLFREKTEKELKDEAENLY
                     LELKPELIIPKLELFASYHLK"
     gene            complement(8836..9654)
                     /locus_tag="P5335_00250"
     CDS             complement(8836..9654)
                     /locus_tag="P5335_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRZ73339.1"
                     /translation="MKLSVIITTYNAEEWLRKVLLGYSVQTETDFEIVIADDGSTSKT
                     AELLKSVELKFKYPIIHVWQEDDGFQKPKILNKAILASNADYLLFTDGDCIPRKDFIA
                     THLQYKEEGYFLSGGYFKLPMTISETIAEEHIYSQQCFDISWLRTQGLKLNFKLTKLT
                     HNKWIALFMNWLTPTKRSWNGHNSSGFKSDILAVNGFNEIMKYGGLDRELGERMFNNG
                     MLSKQIRYSAICLHLDHARGYFSQEEWDKNLAIRAYNRKHKITKTPNGITLNDN"
     gene            complement(9698..10459)
                     /locus_tag="P5335_00255"
     CDS             complement(9698..10459)
                     /locus_tag="P5335_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo domain containing protein"
                     /protein_id="HRZ73340.1"
                     /translation="MDFKINSKLDFPESILLGFIKNFTTEGKILSDGTRNCIKIFSWQ
                     NRSVAIKSFKIPNFYNAVIYRYFRKSKAKRSFEYAIFLSENNIGTPAPIAYFENYSFL
                     GLKDSYYVSENLECDLTFRELVKIPDFPEHEAILRQFTQFCYQLHEKGIEFKDHSPGN
                     TLIKKNEQGLYDFFLVDLNRMNFHEKMPFELRMKNLCRLTPKQEMIVVMSNEYAKVSG
                     ESEKLIFETLWKYTSEFQYRFFRKKRLKKKFLFWK"
     gene            complement(10498..11139)
                     /locus_tag="P5335_00260"
     CDS             complement(10498..11139)
                     /locus_tag="P5335_00260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRZ73341.1"
                     /translation="MDYKNKPTGYYDNFRDEMLVYLPKEAKKILEVGCGNGCFAEVIK
                     KQNNAEVWGIELMEEEAEKAKLVLDKAFAGPCENFIDDLPEDYFDAIYFNDVLEHLVD
                     PYTVLKKLRSKLSKNGVVISSIPNVRYHNTFMRVLIKKDWKYEDYGVMDFTHLRFFTE
                     KSINRMYKEAGYTVKVSEGINKSKSLKPYLYNIPVLFTQLDIRYPQFATVASK"
     gene            11290..12216
                     /locus_tag="P5335_00265"
     CDS             11290..12216
                     /locus_tag="P5335_00265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRZ73342.1"
                     /translation="MIVSSIIIINYNTSKLTLQAIQSIEEKVVDFEKHEVIIVDNASN
                     KEDFSALKIGLKNDFKISIQLIRSRINVGFGAGNMLGVQKAVGAYYVFMNSDVILIED
                     SVNIMIEFLKKSSNVAIVGCQAIDENGKKFKCFDYGLSLTTELFSHSFLHLLNPKKYP
                     SRMLTSKEPTNVGAVPGSLFCCVAKDFDAVGGFDTNLFLYYEEKDLAFRIAKNLKKDI
                     YSLPYSQYIHLKGKSTGTSQVIRNEMKIAQFYVIQKNLNSFSYLLFYFFQFIVFLIKS
                     PFSKKNRAYLILLVKGISVAKSIKHAQKIIQI"
     gene            12213..13322
                     /locus_tag="P5335_00270"
     CDS             12213..13322
                     /locus_tag="P5335_00270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRZ73343.1"
                     /translation="MTILHVSAVKNWGGGENHIENLCFELKKSNSEINNIILCVRNGL
                     FHKRLLKTDLKYETAPLAFNLDIRFAFKIIALCKKNHVDVIHIHDPSAIALTIIADKF
                     YSLPPFIFSKKTSFPIKKKKLTLYKYNYPKIKKYLCVSNETKRVTADSIVDKSKLMTI
                     YHGTNIGTKSSETPFSLREKFAIPNSKKIIGVIGNHIRAKHFETLIEVVNELINIQKR
                     KDFQFVQIGNYTDRTTDLLKKVKEYKLENDIIFLGYTPYASNFIPQFDALLITSQSEG
                     IPQVIFESFYHQKPVISTSVGGIPEIIQDGINGFLAPRHDFKTLAEKIIYLFDNKELI
                     QNLTTISYKNLIENFTTQQMAQKTLEVYKTVSNGD"
     gene            13312..13878
                     /locus_tag="P5335_00275"
     CDS             13312..13878
                     /locus_tag="P5335_00275"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRZ73344.1"
                     /translation="MEINKEIEKAYQVIQNGGIILYPTDTVWGIGCDATNAEAVQKIY
                     ELKKRSESKSMIVLMNGEKMIYNIFKDIPEVAWQILDLSEKPTTLILDKPRNVAPNII
                     AEDDTLAIRIVKEPFCYKLMERMKRPLVSTSANISGQPTPKSFKEISPEIIKGVDYVV
                     NLHREKICDKPSTIIKLTLDSQVKIIRK"
     gene            13916..14305
                     /locus_tag="P5335_00280"
     CDS             13916..14305
                     /locus_tag="P5335_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRZ73345.1"
                     /translation="MEDDYLYKEESYKLVGILFEVHKNLGKGFSEIVYKDALEYEFNK
                     NNIPFVREKEFKVKYKDTYLKHKFYADFVVFDTITLEIKTVECFNVSHYNQCLNYLKI
                     SGYQLALLANFNLISLEYKRIVDSKKK"
     gene            14331..15752
                     /locus_tag="P5335_00285"
     CDS             14331..15752
                     /locus_tag="P5335_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRZ73346.1"
                     /translation="MNYTKALHNPIFKIISQAAQELGVESYVIGGFVRDFLLNRDFKK
                     DIDIVAVGSGIDLALKVSELLPNHPKVQVFKTYGTAMLRFQEIDIEFVGARKESYQTE
                     SRNPIVENGTLKDDQDRRDFTINALAFSLNQENYGELVDPFNGIVDLENRIIRTPLDS
                     DITYSDDPLRMMRAIRFATQLNFTIEEKSFQAISRNKDRISIISGERIVDELNKILLT
                     EKPSVGFILLYQTGLLDLILPELTALNQVEEMEGHTHKNNFYHTLEVVDNICPNTEDV
                     WLRWAALLHDIGKAPTKKFNKKQGWTFHGHEFLGGKMVKQLFERLHMPLNHKLKYVSK
                     LVMMSSRPIVLSQEIVTDSAVRRLLFDAGEDIEDLMTLCEADITTKNPTKFKKYHNNF
                     KIVRQKIVEVEERDHVRNFQPPISGEEIMEIFNLKPSREIGVLKEAIKEAILEGEISN
                     DYEAAFNFMIKRAMKLGLQPIEK"
     gene            15825..16844
                     /locus_tag="P5335_00290"
     CDS             15825..16844
                     /locus_tag="P5335_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRZ73347.1"
                     /translation="MKTNKPVIIWLLSGCTLLFIMVVVGGITRLTNSGLSMTDWHLVT
                     DTFPPLSEEKWGNAFEEYKKFPEYQKINIHNDFTLEDYKFIYFWEWFHRFIGRIIGLA
                     FIIPFGYFLIKKKLDSETLKKCIVLLGMGAFQGFLGWFMVKSGLIDNPDVSHFRLALH
                     LTFAFITFAYTLWVALDLIYPAKNKVILPLRKLARIAMVVLIIQIIYGGFVAGLNAGL
                     IHNHWPLMSEGKLIHETVWIEQPTLFQNLTEGKSGVQFIHRTLAYVVVILMVLLYFRS
                     KKYSLSTTQIKGLNTLVVFVFIQFLLGIFTLLLHVPLWLGLVHQVMAFFLLSAMTFTL
                     HRFSK"
     gene            16891..17661
                     /locus_tag="P5335_00295"
     CDS             16891..17661
                     /locus_tag="P5335_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRZ73348.1"
                     /translation="MSKLAVQDKFLDLSDYGRSFGKFFALQLKNTSFTPIHVTLLFGF
                     TGIVAIYSILEGFYYWAAFFIVLKSGIDAADGELARLKKTPSYSGRYLDSIFDILLNF
                     GFLTAICYVSNTTVWLSFLAFLSIQLQGTLYNYYYVILRTKSVGGDTTSKIFEYKSPK
                     ALPGEKQEVVNFLFKIYSIIYGGFDKIIHFLDKEAYKVKTFPNWFMTLLSAYGLGFQL
                     LIIAIMLPLGWIEYIVPFFIFYTVFIFLLIGIRKQYIA"
     gene            complement(17714..19420)
                     /locus_tag="P5335_00300"
     CDS             complement(17714..19420)
                     /locus_tag="P5335_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HRZ73349.1"
                     /translation="MQFKKIIVTSFLLSFSILFSQEKDSITKKDYGRVYGGFESNAQW
                     YLNDKEIGTIQPEDPVRSNSYLFANYQYKNWTAGVQVEGYLKEALLNYNPEFSGIGLG
                     TYFLNYKTEKVDVTAGYFYEQFGSGMLLRAWEDRALGINTALRGGRFIYRPSSNVKLT
                     ALFGQQRSGFDVSDGKIYGFDSDFQLSEALKLEKSDLSFGLSYVGRYESIEDIPEPNF
                     EALTNAFSGRVNYSINSFYSSVEYNYKSEDAVLNRVSNTIDNDFIKPGNAILLNLGYS
                     KQGLGIDATLRRTENMLFLSERVPTVSGENTSLNFNDKVLNFTPALTKQQHSNLANIY
                     VYQAQVGVDFKSPTILKAGETGGQIDIFYEFAKDTKLGGKYGTKIAFNMSQWYNLPGE
                     YQLTPSNYETNFFGVGTKYFSDYNLEVKKKLSEKWHTGFNYVNQYYNKPWIEDASSDV
                     NTNIITVEAIYNFAETKSIRFEAEHMWADADFKNWAGGTLELDINDTYSFYIWDIINY
                     GNDNPDKRNHYFNLGGAYRINSTRIGMNYGRQRGGLVCVGGVCRFVPESTGLTLTLST
                     AF"
     gene            complement(19420..19905)
                     /locus_tag="P5335_00305"
     CDS             complement(19420..19905)
                     /locus_tag="P5335_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRZ73350.1"
                     /translation="MKKIILFTFLLFNVINAFSQKQMPNVTLTSDTNKSFNVKNDFNE
                     KDKLYVFAFWATWCAPCLNELDAINEYYTTWSTELNMELIAVSIDDTRTQKRVKPLLN
                     GKNWTYTILLDTNQDLKRALGIANVPYTIVVKNQKIVYVHNGYSQGAEMELYNKLKEL
                     K"
     gene            20136..21848
                     /locus_tag="P5335_00310"
     CDS             20136..21848
                     /locus_tag="P5335_00310"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HRZ73351.1"
                     /translation="MKKTLLTAVLLTFGFANAQTFVNTNPENKKVILEEFTGIHCVYC
                     PQGHAIANSIKNANPNNVFLINVHVGGYAEPNSGEPDFRTSFGTAIAGQTGLVGYPAG
                     TVNRTAFAGLNQGALGTTAMNRGNWSNASNQTLNQSSYVNVGVQGVIDAQTRQLTVTI
                     EVYYTGNSPVSTNKLNIALLQNNTLGPQTGGNMGNNYNHQHRLVHLLTGQWGTDITTT
                     TNGSFYTQTFNYTIPASYNNIPAEIGEMELVAFVTETQQKIISGNGAKPTYTGLANND
                     AKVKSIETIGEQCTNSIGPKVTIQNNGQTPLTSLSIDYAINSGATQNYVWTGNLAPLT
                     SAIVQLPAVSYTLLPTNTVTITIPNDDNVSNNSGVINFNKAVETQFTNITVKISTDQY
                     GSETTWNLKNSAGTTVAQGGPYTDAAGAGVFPKPDVNLTLANDCYTFSIIDSYGDGMC
                     CSYGNGSYQVLANGVLIPGMTGGNFGSGESKKYGVNTNLSVSAFDANAIKFYPNPTNG
                     MITISLPEMAKVSITDLSGKLISTSMLEAGDSTLNLSDLSSGIYLINFTGDNFAKTDK
                     IILK"
     gene            21924..22637
                     /locus_tag="P5335_00315"
     CDS             21924..22637
                     /locus_tag="P5335_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ73352.1"
                     /translation="MKKITYSIFFSFLFLTSFAQVEFKKSDGTIITDGMVIPFGTTGT
                     SAYLNFRVKNVSTTPLDIKIKCIGLINTTGANFELCYGGSCFDSVTLNGIYPDYENIL
                     APGQSNPSQGDHFVNFNPGSGGIMDFVFSVYALGFESSAITFTYRYDPALSLINLENL
                     SSLGINVKNTVVFDEFSFTAANKGSVTLFNLNGQLIKELNFSEGSQNINLSNLNTSIY
                     VARFINSEGVSSNVKIFKQ"
     gene            22642..23694
                     /locus_tag="P5335_00320"
     CDS             22642..23694
                     /locus_tag="P5335_00320"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HRZ73353.1"
                     /translation="MNKFGVLGILLIIFSFFSCSTDYKILESVESIGLTTDNSIKVIG
                     ETITFTVTTNSGEIITEDATILVNNTPIDGNTFTTTEIGTYTVQAKYYSVLSEPISVR
                     FHDGSEINFVKRVLIEDYTGTWCGNCPRVSHAIELARAQSEFIVPVGIHRSSSNPADA
                     AYDPYNFDSSALEAVLNMSGYPKALLNRMTRWQPLEQNNITQVINLTQGDNPKLGLSM
                     SNQVTSTNITLDVNIKFSQDFENLKLVVYVLENGLIYDQVNYTAFYGGLPYITNFEHE
                     HVLKSCLTPIFGEPIASEETKVGNTYTKSFSISIPSNIANSNNIEFVAFVTGDDNKAI
                     NVRKANRNENQEFEEF"
     gene            complement(23746..24501)
                     /locus_tag="P5335_00325"
     CDS             complement(23746..24501)
                     /locus_tag="P5335_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRZ73354.1"
                     /translation="MRIIIIEDEKPAARLLQRKLEKLGLQVDTLLHSVEESLQWFQNN
                     AHPDLIFLDIQLSDGLSFEIFEQIDIKSAVIFTTAFDEYALRAFKLNSIDYLLKPIDE
                     DDLEIAVNKYKSRNQALPNLSLDFDMIKRMLVNPVEKTYKQRFTIKIGERLKMIPIEE
                     VECIYSENKGTYLHTLDNRDYLIDGTLDHVETELNPNQFFRVSRKFIIPLKSIKDILV
                     YSNSRLKVILPTYKEDEVIVSRERVNDFKEWLG"
     gene            complement(24541..24849)
                     /locus_tag="P5335_00330"
     CDS             complement(24541..24849)
                     /locus_tag="P5335_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HRZ73355.1"
                     /translation="MNAHDEIKYQEALKRVKKIKGFYTHAIVYVIINLMIIIVNYQDL
                     DPGESYFQYKNFTTLFFWGIGLAAHGFSVFVPQWFLGNNWEERKIKELMDKDKNSKWE
                     "
     gene            complement(24864..25163)
                     /locus_tag="P5335_00335"
     CDS             complement(24864..25163)
                     /locus_tag="P5335_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HRZ73356.1"
                     /translation="METKEEYERYQKASKKVQEIKGFYSHLIAYLFVIPIIAFVNIKY
                     TPEHFWFFYPMIGWGIGVLFHGIGVFGTDTMFGSDWENRKIKQFMEEEKNKDKYQ"
     gene            complement(25175..26512)
                     /locus_tag="P5335_00340"
     CDS             complement(25175..26512)
                     /locus_tag="P5335_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HRZ73357.1"
                     /translation="MNKLIKEFPRATFILLGVFVVLTLLNVVMGGHVAWNIELAMSFG
                     FTMLYGYSLYYANAILFMQLDNYFKEERFSSKRLFFGAFASFLVSILVIFLLRIFEDV
                     IIEGKSLEAFLATERPANYLVAIVVTFFVTLGIHAFYFYKAYQENKVKEQKVIAGTAS
                     AQFESLKNQIDPHFLFNSLNVLSSLIEENPENAQKFTTSLSKIYRYVLEQKDKELVPV
                     AEELAFAKTYMNLLKMRFENSITFELPDGFNNEEAKVVPLSLQLLLENCIKHNVVSES
                     KPLHIKIYIENNQLVVENNLQKKEVLSDRKGVGLQNIVNRYAILTQRKVLVEETEKSF
                     KILVPILTKQISIMETQMKYNENTAYLRAKDRVEKLKGFYGNLISYCVVIPVLIIVNL
                     NTSNFQWFWFPMLGWGMGLTFHALETFGYGKSWEERKIQEILEKEDKQKNKWN"
     gene            complement(26520..27140)
                     /locus_tag="P5335_00345"
     CDS             complement(26520..27140)
                     /locus_tag="P5335_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73358.1"
                     /translation="MTKIIVTIVLFVTAQLFSQGQYEQGMGKAFALWGEGKNTEASSM
                     FERIASAEKDNWLPNYYVALVNTTTAFTTKDKDQINALLTKAQTTLDVEITKQPNNAE
                     LIVMQAMIHTAWIAFDPMTNGMKLSGKVMELYGKAEAIAPTNPRVVFGKAEFEMGSAK
                     FFGTDTQPICAQIAKSIELFATFKPESAFHPNWGLERAKEALTACK"
     gene            complement(27301..29454)
                     /locus_tag="P5335_00350"
     CDS             complement(27301..29454)
                     /locus_tag="P5335_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ73359.1"
                     /translation="MKTCLTLAFVFVYFLSFSQNVVSGKVTDEKGGPIPGANVYLEGT
                     YDGGSTDENGYFSFTTKETGTKTLIVSFLSFETYAKEIDISQFQNQIIKLKESVNVLD
                     AVILTAGTFEAGDKARVSVLKPLDIVTTAGAVGDIIGALATLPGAQTVGESNRLFVRG
                     GEADETQTFVDGLRVAQPYGATANDLPTRGRFSPFLFSGISFSTGGYSAEYGEALSSI
                     LSLNTNDDVLQEQTEISLMSVGLGLGNTQRWNKSSLTFNMSYINLAPYQLLVPQEVDW
                     NKPYQSLTGETVFRRQFKNGLLKIYASLDVSDFDLNQEDINQPEKVRFDLKNNNFYGN
                     ASYKGTFGNNWSLQTGVSYGISLNEIGINTDIVQNAEYATHLKAKVSKKITERIKLSG
                     GADYFVTKFDEDFIPFEQTTFSSGYDNTIAAVYAESDIFFSKKLALKLGVRASDNQLL
                     DEFQVTPRASFAYKVSKNGQFSFAYGDFVQTPRQEYIKYETNFKSEKASHFILNYQYN
                     KEGRTLRAEAYYKSYNDLVTFNSQQPQFDSEYNSNGDGFAQGLDIFWRDNKTIKNTEY
                     WVSYSFIDSERLYRNYLTAVQPSFVADHTLSIVTKHWINDWKSQIGFSYTVNSGRPYN
                     DPNEADFMNGLTKPYQNLSFNWAYLISQQKILYFSVSNVLGTENVFGYQYANTPDLNG
                     DFQRRDIGQPADRFFFIGFFWTISEDKKSNQLDNL"
     gene            29581..30159
                     /gene="nudK"
                     /locus_tag="P5335_00355"
     CDS             29581..30159
                     /gene="nudK"
                     /locus_tag="P5335_00355"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HRZ73360.1"
                     /translation="MNPKIQITKTELLSDNWYLLNKVTYNFTPENGQPETQRREVYDR
                     GNGAAILLYNKENQTVILTRQFRLPTFLNGNENGMMIEVCAGLLDEKNPEDCIKRETE
                     EETGYRIQDVKKVFEAYMSPGAVTEILYFFTAEYQPEMKVSEGGGLASEHEYIEVLEL
                     DFNQAMAMVYNGEIKDAKTIMLLLHAKNLQLL"
     gene            30246..30782
                     /locus_tag="P5335_00360"
     CDS             30246..30782
                     /locus_tag="P5335_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73361.1"
                     /translation="MIYKFRVILDTEEDIFRDLAIECTDTLEDLHNAIVNAFGFDGSE
                     MASFYTCDDNWNQEDEIPLFDTGDVPGEMRIMSDLTLEDMLYEKQTKIIYVYDFLNMW
                     TFLVELAAIEEKQAGATYPELLYSLGLMPLAANDPTFEADDDSLFTEFEDELDEDDLD
                     MFEGDDSFEDFGFEENWN"
     gene            30949..32007
                     /locus_tag="P5335_00365"
     CDS             30949..32007
                     /locus_tag="P5335_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HRZ73362.1"
                     /translation="MINLFNAHIESLSIHRVGNKSRNEAIFLSEQPYQTNDEIVPLLK
                     EFFLKSFREKEENYYQFAHEVDLEYHDMFKLATEVFDNPSTIHEVSKKITKHLFEQSN
                     HPHIKNGEVYVAHFTHLTIDNNQVDAIGVFKSEVQADFLQFEEKASNLEMHLQHGINL
                     NKLDKGCLIFNYKKEEGYKILTVDSNRYDARYWLEHFLNVDAFQDENFITKKYLKFCQ
                     DFAKDVVFPAEDKKQEVMFMNRSVNYFAKNDEFEETNFLNEVMENPDLIPEFKSYKVD
                     KGEKYSIEDVTNFPIANAAVSDARKKIKNVINLDTNIQIKLDFINPESAEKYVEKGWD
                     EEKQMYYYLVYFNKEQKS"
     gene            32196..32444
                     /locus_tag="P5335_00370"
     CDS             32196..32444
                     /locus_tag="P5335_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73363.1"
                     /translation="MKTYITPEPSPGTVFICTLIGHKYQTTRKITNHFSEYKCKCCGK
                     QMTDDGRGRLTLLTPELHEINETLKMIFHKKSQHLQSA"
     gene            32595..33491
                     /locus_tag="P5335_00375"
     CDS             32595..33491
                     /locus_tag="P5335_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRZ73364.1"
                     /translation="METILTIEKLNKRFGPVHAVKNVSLEINKGNVYGILGPNGSGKS
                     TTLGIVLNVVNKTSGEYRWFGGAMETHEALKKVGAIIERPNFYPYMTAQENLELVCKI
                     KGTPYSKVTEKLELVGLIDRKDSKFRTFSLGMKQRLAIASALLNDPEILILDEPTNGL
                     DPQGIRQIRDIIKHIASQGTTILLASHLLDEVEKVCSHVLVLRKGQILYSGKVDGMIA
                     NEGFFELQADNLEELKAALIAHPAIEKMVLEEGKLVVYLKQAFEAKDLNKYLFEKGIY
                     LNHLVKRKHSLEEQFLELTSKN"
     gene            33526..34365
                     /locus_tag="P5335_00380"
     CDS             33526..34365
                     /locus_tag="P5335_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRZ73365.1"
                     /translation="MTRLLQIEFQKIWKNRASRILTIAYFVILSFIALIASIKFKIGT
                     FELHIADQGIFNFPYIWHFNTYIAAILKFFLAIVIVSMMANEYSYGTLKQNLIDGLSK
                     KEFIQSKFLTVVVFALGSTLFIFIMSLLLGLSFSSYNEIGIIFSDLDYLFAYFLKLTA
                     FFSFCLFLGILVKRSAFAIGFLFIWNIIEGIIIGLLRWKVFKDSDDYQKITQFLPLES
                     MSNLIKEPFSRLSVIKNIEETIGGAANVKDYGVHWYSLLIVLCWIVIFVLMSYKILKK
                     RDL"
     gene            34382..36676
                     /locus_tag="P5335_00385"
     CDS             34382..36676
                     /locus_tag="P5335_00385"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRZ73366.1"
                     /translation="MKFAKNILFFLLLIIAFQSGKAQYIQIDDNQSAQELIENVLINS
                     SCANVSNITVSGWAFTNGNSYGYFNRGTSTFPFQDGVIIATGRALSAIGPNSSLLSEG
                     PTNWPGDIDLQQAINETNTINATVIEFDFLPFANKVSFDYIFSSEQYLSNPNSNQCSY
                     SDGFAFLLKEVGSQDQYTNLAVVPGTAIPVKVPTVRGAGTICPPANEAYFDAFNGIEH
                     PTNYNGQTKILKAESLVTPGVLYHIKLVIADQGNNLYDSAIFLGGGSFTVETELGDDR
                     LIANGNPLCENETLTLDATTPNAVGYKWYRNNVLINNETNQTYTVNSFGVYKVEVVFN
                     TTCSSFGEIVIEYSSNPIPNDVNLTQCDSDMDGLAIFNLNDANNSIIGSNLNVGPPKY
                     FLSFNDAFTVQNTLSSIDSFQSTTPNQIIYARLENTFGCFGISEVTLSTSISSVIVPP
                     ISICDGDTNQDGITVIDLSMNISPTVLGYFPTGLQAVYYSSTENAQTQTNALPNNYTN
                     TIPFEELIFARITDGVNCFGILPITLKINIFTPNNFETEELSLCENETYFLEVEEIYE
                     SYLWNTGEITPRINISVPDTYTVTVINSDGCEATKTFIIIPSGVATITSIDVQDFSGN
                     QNTVQINYTGLGDYLFSLDGNYFQNSPIFTNVEPGEYLVIVKDTNGCGTVTQQIFVLD
                     YPRFFTPNGDGFNDFWEIKNFNQPNSKIYIFNRYGKLIKEIVPNQKGWDGTFSGSLLP
                     SSDYWFSLFLENGRIIKGHFALKR"
     gene            36710..41656
                     /locus_tag="P5335_00390"
     CDS             36710..41656
                     /locus_tag="P5335_00390"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HRZ73367.1"
                     /translation="MKKIALFFLLITLNCFGQFSKTHYIPPLGGSTGQPAQNQYMYIS
                     SPSLTPVNFTINSLGGGTVSGTVSRNSPYVYTIGFGTDSQLMVSSSSMSAVLNNKGFI
                     VEAEDQVYVAVRLTASPQNFQAGGLVSKGLAALGTQFRIGAFVNTGISGITNNHLTFV
                     SILATENNTLVSFSDIKPGVTLINNGGAGNNPAAITLNRGQSYVMATTGPSVPNTDGL
                     IGALVSSDKPITVNCGSFAGTNGNVDNNLDLGFDQIVSAERTGNEYIFVRGFGIDITE
                     RPLLIAHENNTEIFLNGNTGTPDYTINAGQYIALDGSNYSSDGNLYVNTSKNIFAYQG
                     VGGSTQANQEMYFVPPLSCQTPKIIDNIPLINLIGNISYTTNSGVNIVTETGANIDFI
                     LNGTNYSLASLPAFINVQGPFNVQGNTNYITYKLTGFTGNISVFADKQLYISYFGSND
                     AATYGGYYSGFTFKPEISFNRLDVTSDNCIPNVNLSINSLSPFDTYQWYFNGVPIAGA
                     TNPNYTPTVPGLYYLSATVTTCNITLISDEIPVSSCPEDTDGDGTYDNIDLDYDNDGI
                     ANCTESYGNLDLNLLNTTTGTITSGNYSNSFTGLITTAGTALPTTNPFIGAVDGSFIT
                     NPAIGIGNSVSYEMNFSQPVSLSMEYVSTANSTDLLSSTGEFIIQCAINKTITILNPT
                     GQLLIDTNYDGIYESGVTEYSSFQIRFRLNSTIPLAAGSGTFKLKSYLTDFLSIKHIN
                     ISEGSSKATFKILATCVPNDSDSDGIPNQFDLDSDEDGISDNIEAQGQNFIAFNPIDA
                     DGNGISDVYGNGLFPIDTDGDGVLNYVDLDSDNDGIYDSVETAIDTNGDGIGNYLTLD
                     SDGDLCNDVIEAGFIDENSDGLLGNGSIVVNSFGVVTNAPNGYTPPNLNYIIGAPIAI
                     TAQPQNQFTCNTQNSSFTFTGTVSDSYQWQLSTNGGATWSVIFNSAVYSGATTTTLQI
                     TGATTSMNNYRYRVLLNRVGNSCGLLSDGATLTVYPLPVLTSPVSIVQCDNDATNDGI
                     SEVSLIQKNDIISANAANEIFTYFTTQLAAQTNNSAFQISNPLTYNTGNTTVWARVEN
                     SDGCFEVGQIDVFVSATQIPNSFIRKFSKCDEYFDVVNDDRDGVSQFDFSSVTAEISG
                     LLPTTTPFTIKYYKTEADALAETDINGNSLEINPSNYQNIGFPGFQQIWVRVESSLDN
                     ACYGLGPFVELTVEELPVANPIPDFISCDDNQDGKFPFNTSSIETTLLGTQNPSIVTI
                     TYFDQNNISLPSPLPNPFLTGSQTIRLRVTNNATSDPNGPCYDETTLTFKVDILPVAN
                     PVPDFTTCDIDGIEDGLFSFDTSIIESTLLNGQTGMIVTYFDENNNSLSSPLPNPFVT
                     KTQTITAVVTNPINTSCPATTTIDFIVYPLPQLADGFKEIICFGVDAITFDAGLLVGN
                     SNDFTYQWFKDDVLMIGENNYQLTVNQNGIYKVIVTSAFGCEQTRTIEIVYSQPATID
                     DIVVVDLVENNSVTILVSGSGSYVYSLDSANGLFQTSNVFENVIAGIYNVYVKDLLGC
                     GVVSQEIPVLGAPKFFTPNGDGYNDTWNVRGVSAKYYANTIIYVFDRFGKLITQVDPK
                     GEGWDGTFNGQNLPSTDYWYSIKFEDGRMVKGHFALKR"
     gene            41659..42576
                     /locus_tag="P5335_00395"
     CDS             41659..42576
                     /locus_tag="P5335_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRZ73368.1"
                     /translation="MKKTIVLYLLLFSSMVSFAQVTKVCAGNIEIYQNFASNFVAPRN
                     VSVWLPENYYPKKKYAVLYMHDGQMLFDSTKTWNKQEWGVDECITKLFRQGKIKNVIV
                     VGIWNTDNTRHPEYFPQKPYESLSVKEKETVSNSLQKRGITVPFKPFSDNYLKFIVEE
                     LKPFIDKTYSTRKEKENTFIAGSSMGGLISMYAMCEYPEVFGGAACLSTHWPGTFEIE
                     NNPIPNAFINYLKTNLPDSKSHKFYFDFGTGTLDAMYPPLQAKVDEVMQTKGYTNNNW
                     ITQKIEGAEHSENAWRVRLHIPFEFLLKK"
     assembly_gap    42751..42850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            42900..44573
                     /locus_tag="P5335_00400"
     CDS             42900..44573
                     /locus_tag="P5335_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73369.1"
                     /translation="MKKYFSFKFSFLFSTLLLILISESGFAQLAVPFKVRYQSHVKGD
                     MTIIANTIVNRTESKNATSEPYNDISEKAKLNDEFEMAYIDIDNDETTFSSSSAQLNL
                     TTSTTKKVIYAGLYWSALYKYESGIRDKKWNYIAVDNKRTSVNEIKFKLPGKSDYIDI
                     AGEVLFDGLEVKNFKESAPYAAYADVTRYIQSLENPNGDYTVANIKATQGIISGGVAG
                     GWSLFIVFEDDSETEKFITSFDGFVGITTRPVEISFTGFSTLPEGKVNAKLACAALEG
                     DRNLTGDQLEIKSGDNLKYSLLSHPLKPADNFFNSTIIINDDYFELRNPNSLNTLGYD
                     TCLLQINNQNNAIIQNNSTKVSLKLKSSGDRYSMFFSAFNVEVSDPLKKIDSAELVST
                     EKKQIELTNNIEINPKTEPTTVATPIVEETKATDRPQRTYYSIMDMIRESEKMNSETV
                     TANGKKRITVLPGADVLIPRQQKGYYVIANVFAKPTNTTRFISLLKNKGLEPEILFNP
                     STNLQSVYLARFNNWEDALSFYYSNADGKYFDDLWIMLVNTSTGTILEP"
     gene            complement(44636..>45895)
                     /locus_tag="P5335_00405"
     CDS             complement(44636..>45895)
                     /locus_tag="P5335_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HRZ73370.1"
                     /translation="DNVEALLDMVVENVPAPKVSEGTPQMLITSLDFSSFTGRIAIGR
                     LERGVLKEGMPISLVKRDGKVIKSRIKEIHTFEGLGRKKVTEVIAGDICAIVGVEGFE
                     IGDTIADFENPEALQTIAIDEPTMSMLFTINDSPFFGKEGKFVTSRHIRDRLTKELEK
                     NLAMKLGETDSADKFMVYGRGVLHLSVLIETMRREGYELQIGQPQVIIKEIDGQKCEP
                     IEELTIDLPETLSGRAVEFVTMRKGEMLSMETKGERMIVKFNIPSRGIIGLRNQLLTA
                     TAGEAIMAHRFIGYEPYKGEIAGRNKGSLISMEKGKAIPYSIDKLQDRGKFFVEPNTE
                     IYEGQVIGENSRGDDMSVNVTKEKKQSNVRSSGNDEKARIIPPIIFSLEEALEYIQKD
                     EYVEVTPKSIRLRKIYLNETDRKRFKI"
BASE COUNT        15528 a         7636 c         7183 g        15448 t
ORIGIN      
        1 tctccggtta atcgattgaa agttacatta tatgttccgg caacggttgg aatatttgaa
       61 ccgttttgtg ttccagagcc tgccgggaaa tcagtacttc cccaattagt tgcccacgat
      121 gcatttgttc tgaatttaac aaagccatca actaatgtaa tgttttcaag tgtgaaagta
      181 acaccaccat caagtgattg taaaggaata tctgtttccc aaccttgagc accatctcca
      241 atgatagaaa ccggaacttg aataaacgtg taatttaaac cactataatt aaaagtgaca
      301 ttgtaaaatc ctgaagttaa aggaatattg cttccaccgg gaacagcttc accagatgga
      361 aaagtagttc caccccagtt tacttcccat gaattatctt tacgaaattt aacttcattt
      421 gctatgaatt ggtagtcatc tttggtgtac agaattccgt caaaagttgt caaagcaaca
      481 ctttcagtcc attcattaaa accacctatc ataccaatgt tgtcaaaaac agattctact
      541 tgtgtaaata tgtaatcacc tgtttcttta ttaaaaacga catcatatgt tcccattggt
      601 acagggatat ctgccccatt ttggactcca actccacttg gaaaagttgc agctccccaa
      661 tttacagccc aatcattgtc ttttctgaat ttagctccac cggataccgg aaaattttgt
      721 gatgttaccg tataaataat accatccgat gtattcatcg gaacatcaac agaccaatcg
      781 gtaaactgtc cgattattga aatattatca aattgtgcaa agacaaagga aacattcaac
      841 atcattaaaa tagtaaaaag taattttttc atcataattt gtttattaag gtttaactaa
      901 aaacaaatat ataaatgatt aaagaattaa caattttaca gataacgaat tgtaaaatat
      961 ggaaataaat gccttaattt atcctattaa ttacataatt gtcaaaaaaa ataggtaaat
     1021 caacgtttta attaaaaaat atacgaaaac gttttcgttg ctgattttca gtattttaca
     1081 gcttctttgc ttcttcccaa aacacatcca tttcggctaa cgtcatatct accaagggtc
     1141 gtcctaattc acttgctcta ttttcaaggt acataaagcg tttaatgaat tttttattag
     1201 ttcgctctaa agcgtcttca ggattcactt ttagaaaacg ggcgtaatta atcattgaaa
     1261 ataaaacatc gccaaattca gcctctattt tatcttgatt tccggaaatt acttcttcct
     1321 gtaattctga caattcttct tgtactttat cccatacctg atgaggttct tcccaatcga
     1381 aaccaacacc tttcacttta tcttgaattc tactggcttt tactaaagcc ggcaaacttt
     1441 ttggaacacc ttctaaaact gattttttcc cttctttcaa tttcagtttt tcccaatttt
     1501 gtttcacttc ttcttcattg gcaaccacaa catcaccgta aatgtgggga tgacggtcaa
     1561 ttaatttatc acaaatactg ttggcaacgt cagcaatatc aaaagcatct gtttcactac
     1621 caatttttgc ataaaatacg atatgcaata ataaatcccc caactctttt ttgatttcag
     1681 ttaaatcatt atctaaaata gcatctccta attcgtaggt ttcttcaatg gtgaggtgtc
     1741 ttaaactctc aagtgtttgc tttttatccc aagggcattt ttcacgcaaa tcatccataa
     1801 tatccagcaa gcgaccaaag gcttcgagtt gttgtgttct tgatttcatt tttttttcta
     1861 tttgtgtaaa aataaaaaat cctactcgaa agcaggattt ttataaagat tttatttttt
     1921 aattactcag ccgtttcttc tttcacttct tgcgataaag cttccattgt tactaatcct
     1981 tttggttgta acatattata ccaattgatg atttttttga tatccgaagc ataaactctt
     2041 tcttcatcgt attccggcaa aacttctcta aagtaggaac ttaaaacacc attatcttct
     2101 ttatgagata tcgatggtcc atcgttttcc tttgtagcaa ttgctttaaa aacttcagct
     2161 aattttattt cttcatcata cgtataaaca gcaatttcag aaagcaaact cacattactt
     2221 cgcattccaa ccgttatttt ttttccgtct aagaaacttt cagctacaaa tcctgttcgg
     2281 gtttgtattt tcaattcaaa taagcccggc tttccggaaa tggctaatat tttttgtaaa
     2341 ctcattggtt ataaataaat taaactttgt ttttatcttc cttttttatt gaaaaatttc
     2401 attttatagt cggctctaat tttaccttcc atgatatttt gcaatctttt tttaattaac
     2461 gtctttttca aagaagatat tttatctgta aataaaattc cttcaatatg atcgtattca
     2521 tgttgaatta cccgagcaat caacccctca aacacatctg ttttacgaac aaaatcttct
     2581 tcacaatatt caattgtaat tttttctttt ctgaaaacat cttcacgaac atccggaata
     2641 ctcaaacaac cttcgttaaa tgcccattca tttccttctt ctttgataat tttagcatta
     2701 atgaaggttt tcttaaagga ttttaattga tttttttcat cagtttttaa atcatcgttt
     2761 tcactgaaag gttcagtatc aatgataaac atcctgatag ataatccaat ttgaggtgcc
     2821 gccaatccta ctccataagc gttgtacatc gtggcataca tgttttcaat taaaatatcc
     2881 aaatttggat aatccggtga aatttcaacg ccgacttttc ttaaaaccgg atcaccatat
     2941 ccaataatcg gtaatatcat tttgtagatt taaaattttt agatttcttt tttttgaaat
     3001 cattttgcaa aagtaaacaa aaaatcaaat ctgtaaagcg tttgccaatt tttaggactt
     3061 ctgatttaaa catttttaac aaaaatcaat tagagcatta ataaattatc ctatatttgt
     3121 tcctatagaa ttttaatata aaacaaacaa atagcttatg aaaaattatg tttatttaat
     3181 tattttcatg tttagtatta acacatttgg gcaagttact gaagaaaatg accaagcaat
     3241 tattgaaaaa gctctttatt ctaaacaaga aaaagaggat atgcttgcta aatacaaaac
     3301 ggatgtagat atcattggta tgactccgga agtaaaagag ctttatatga atcttattac
     3361 ttcaaatttt caaaaaatga ttattttaaa taaagacaga aataatactc aaaaagaatt
     3421 aaaacaacgc ttaaaagtag tcctagaaga ccaacgtgtt gaactaaaaa aattattaac
     3481 ttacgaacag ataaaaagac accaacaaat ttataaaccg attattgaaa gtatcagatt
     3541 tcgaattgat tcctataaat aaaagttaaa tcatctttct ggttaaataa tcctgaagaa
     3601 gtattgttgc agctatttca tccactaaac ctttgttttg acgttgtttt tttgataaac
     3661 cactgtcaat catcgtttga aaggccattt ttgaagtaaa tcgttcatca acccgaatga
     3721 ctttcatgct tggaaattgt gttttgaaat ctgatacaaa tttttcaata atcggagtgc
     3781 tttcagaagg taaaccattc atttgttttg gctctccaat tagaacttca atcacctttt
     3841 cttttgaaaa atactctttt aaaaaattta taacagaatc tgaaggaaca gtcgttaatc
     3901 ctgaagcaat aatttgcaat tcatctgtaa cggcgatccc agtgcgtttt aatccgtagt
     3961 ctatggctat aattctaggc attctctttt ttattcaaag gtacaataca aaaaacaaaa
     4021 tcctcaattg aggattttgt tttatttttt aatggcttca aatcttgcca aaggagccat
     4081 tcgggcttta ttcaacgtca tttgattacc attgaggcta taattatcta ctgtttccag
     4141 cattttagca aattcttgtt cggtttccat atccgggcaa gccatcatgg tggaagcacc
     4201 tttagaaaat ttaattcgaa aaatttcttc tttcaattca tactgaccca tcatgctatt
     4261 acaacctgca aaagcggaat aacgattttc tttatttaat tgcaaaaaca agtcttttgt
     4321 agattgattt atatttttta tggcacgtcc gtttaattca actaatctcc attttgttcc
     4381 tactacagca taaacctctt ttcccacttg tttttgaaca gaagttgagg ttggagattg
     4441 attaaaatta ctttttgttg gcggagacgg tggagcaatt tcaactgcgg tttcattctg
     4501 tttttcaaga acatacttag aagctaaatc tcctgtaatt ttatttcctt ctaaatctaa
     4561 ttgagttaga taattctcac ttacaaaata ttgagaaggt ccgttttcta taccagataa
     4621 aactacagta tttccttctt tattccatgt atatgtccct tttagttcat ttatttttga
     4681 gtctttttta cccaaatagt gtgtagtcaa aacaaaatta gattcatccc tcaaattcaa
     4741 aattgtctca attccctcac aatcggcaca aggaacaatt cctttataca taccaatata
     4801 atcgagtgaa ttcttagcat tatgttcatc gattacaatt atcgttgttg aatcatttaa
     4861 aatttcatta gttgcttctg ttttggtttt tttggtacaa cccacactaa aaaataggat
     4921 tgcaatacaa gtgatggaag taattttcat aactattgat ttttattata acaaatttaa
     4981 agctttttgc tatctaaaaa tcaatcttgc aaaaatcaaa ctactttttc ttaacaaaag
     5041 attttatctt tgctcaaaat aaaataacat gactgaatta aaaaacacta tagaaaaagc
     5101 ttggaacaac agagctttac tccaagaaga agcaacaatt acaactatta gaaaagtcat
     5161 tgatttattg gataatggaa aactacgcgt agctgaacca accaccgaag gttggcaagt
     5221 gaacgaatgg gtaaaaaaag cggtagtcat gtatttccca atccagaaga tggaaacttg
     5281 ggaagccggt atttttgaat accacgataa aatgttatta aaacacaatt atgctgaaaa
     5341 gggaattcga gtagttccac cggcagtagc tcgttatgga gcttacatat ctaaaggagt
     5401 aattttaatg cctagttatg tgaatattgg agcgtatgtc gatgaaggca ccatggtgga
     5461 tacgtgggca accgttggca gttgtgctca aattggaaaa gatgttcatt tgagtggtgg
     5521 cgttggaatt ggtggtgttt tagaaccact acaagcagca ccggttatca ttgaagatgg
     5581 agcgttcatt ggttcaagat gcatagtggt tgaaggaatt cgggtagaaa aagaggctgt
     5641 tttgggagct aatgttgttt taaccggttc cactaaaatt attgatgtga ccggaagtga
     5701 gcctattgaa tataaaggat atgtccctgc acgttcggtg gtgattcccg gaagttatac
     5761 caaaaaattt gccgcaggag attatcaagt tccttgtgct ttgattatcg gacaacgtaa
     5821 accctcaacc gatttaaaaa cgagtttgaa tgatgcgttg cgggaatata atgtggctgt
     5881 ttaaattgtt tttttaacca aatcattata ttattttgta caaagattta atcaaattat
     5941 tactaaaacg attactgatt gttgctttct tataccaagt ttgcagaatt ttattttatg
     6001 cttttaatcg gaatgctttt gacaaattgg atctttttac ttttttgggt ggacttcaat
     6061 ttgacttatc tgccattggt tatatcaatt tgatttttat tttaggtcac ttaattccag
     6121 gtaattttaa atacaaaccc atttatcaaa aaggcctaaa aatttctttt tatagtgtaa
     6181 acttgctttt tatagctaca aactttgtcg attttgaata ctataaattc acgggaagaa
     6241 gaagttcttt tggattaata accgcaacag gaatggaaaa tgaaataggc gttttattac
     6301 tttcgttcct taaagaattt tggtatttac cattacttct gattgctatt ggaatcttat
     6361 tttggaaatc aattggtaat tttaaatccg gaaattcaaa ttcaacatca aaaagacatc
     6421 atttaaaaga attcgttatt ttgattattg cattaggttt agggttatta atgggtagag
     6481 gcggatttag aaaaaaaccg ctcaaagtag tagatgccat aagttatagc ggaaccggaa
     6541 attctgcttt gattttaaac acccctttta ccatactaaa aacgattgga aagaaagaaa
     6601 cgttggaaag ccctaaattt tatgaccaaa ccacattaga ccgcattttt aatccggttg
     6661 taaaaacaaa tccttcggga gaaagcacta aaaaaaatgt agtgattatt attttagaaa
     6721 gttttggaaa tgaaaatatt aggattggtt atacgccctt ttttgattca ttagcaacac
     6781 attcttatta tttcaaaaac ggatttgcaa atggtaaatt atcaatcgat gcagttcctt
     6841 caactttatc gagtatccct agcctgatga atcattcgct catcacttct aattattctt
     6901 taaatgaaat atatggttta cctaaaattt taaaagacaa tggttatgca acatcttttt
     6961 ttcacggggc ttttaatggg agtcaaaatt ttgaccaata ctgtaaagta gccggttttg
     7021 ataactattt tggaaaaaat gaatatgaag gaccggaatc atttgatgga acttggggtg
     7081 tatttgatga agagtttcta cagttcttct gtgaaaaatt aacaagtttc aaacaacctt
     7141 ttttctcctc agtttttact atttcttccc acaatccgta tactattcct aaaaaatata
     7201 atgggaaatt cccgaaagga aaaactaaaa ttttagaaag tattgcctat tccgattatg
     7261 ctttaaaaca attttttgaa acagcaaaaa aacaagaatg gtataataac actatatttg
     7321 ttttaactgc agatcatact tcttccgatc caatattagc taagcataaa acaaatgttg
     7381 gcaaattcag catacctata ttattttttg acccctcaaa tccggaatta aagggagaaa
     7441 ttgaaaaaaa tatccaacaa attgacatta tgcccagttt gatagattat ttaaacattg
     7501 aaacaaaaat ggtaacttat ggcaaatctt ttaagtctaa aaagaatttt gtagtttatt
     7561 ttttagataa tatttaccac tacataaatg atgattatta catggctttc gatggaaaaa
     7621 aagctattgg attatataat tttaaaaccg atgaattact gaaaaacaat cttttaaaga
     7681 gcaattctgt tattgcagat caaatggaaa aattcatcaa agcttatgtt caatcgttta
     7741 acgaaagggt aataaataat aaattacatg ttcactagca ttttcgaatg aaaaaaatat
     7801 tagttataca acaaaaaatg attggcgatg ttttactaag ttcaatctta tgtaattcac
     7861 ttcgaaaggc atatcctact gcccaaattg actatttagt acatgaaaac accgttccgg
     7921 ttttagaaaa caatccgaat attgacacta tcattcgttt tactgaaaaa cataaatcca
     7981 gcagacgtgc ttttttaaaa cttggtaaaa aaataagagc cgaaaaatat gatattctga
     8041 ttgacgccta ttccaagttg gaaagttggc tcctttccgc gatgagtggt gcaaaacaaa
     8101 aaatatcgta taaaaaaatt ggtcgtaatt tcatatatga tattaatgtt ccctatgaag
     8161 atgctccaaa aacaagttta ggactagcaa ttgaaagaag gctttcctta ttaaaacctt
     8221 tatcaattga aaaatatatt aaccccattc caaagttatt tttaacaaat gctgaaatgc
     8281 aaacagctaa aaatttattt aaaaaatttg gaataaatga aagtaaaaaa acggtcatga
     8341 ttagtctttt gggaagtgag gaaaaaaaga cttatccatt accttttatg gcaaaaatta
     8401 ttgatttcat tggtcaaaat tatgatgtaa ctattttatt caattatttt ccaaagcaaa
     8461 ttaaagaagc caaaatcatt ttgaatttat gttcagaaaa aactaaaagt aaaatatatt
     8521 ttgatttttt aggccaggat ttgagatcat tcattgcaat catgaatgaa tgtgacgcca
     8581 taataggaaa tgatggcgga gcaattaata tggcaaaagc cttaaacaaa ccttctttta
     8641 ttattttttc gccatggatt gaaaagaaaa tgtgggctac ttttgaagat ggaataaacc
     8701 attcttctgt acatttagaa gattaccaac ctaatctttt tagagaaaaa acagaaaaag
     8761 aattaaaaga tgaagcggag aatttgtatt tagagttaaa accggaattg attattccga
     8821 agttagagct tttcgctagt tatcatttaa agtaattcca tttggtgttt ttgtgatttt
     8881 atgcttgcga ttataagctc tgatggctaa atttttatcc cattcttctt gactgaaata
     8941 acctcttgca tggtctaaat gcaaacaaat agcactatat cgaatttgtt tagacagcat
     9001 tccgttattg aacatacgtt cccctaattc tctgtctaaa ccaccgtatt tcattatttc
     9061 attaaaccca ttgacagcta aaatatcgga tttaaaacca gaagaattat gtccgttcca
     9121 tgaacgtttc gttggtgtaa gccaattcat aaataacgca atccatttgt tgtgcgttag
     9181 ttttgtcaat ttaaaattta actttaaacc ttgagtacgt aaccaagaaa tatcaaaaca
     9241 ttgttgagaa taaatgtgtt cttctgcaat ggtttcagaa atagtcattg gtaatttaaa
     9301 atatcctccg gaaagaaaat aaccttcttc tttatattgt aaatgagtag ctataaaatc
     9361 tttacgtgga atacaatcgc catccgtaaa caataaatag tctgcattgg aagctaaaat
     9421 ggctttgttt aaaatttttg gtttttgaaa tccatcatct tcctgccaaa catgaataat
     9481 tggatattta aatttcagtt caactgattt taaaagttca gccgtttttg aagtagaacc
     9541 atcatcggca attacaattt caaagtcagt ttctgtttga acgctatatc caagtaggac
     9601 tttacgcaac cattcttctg cattgtatgt cgttatgatg acggataatt tcattaattc
     9661 ctttaagaat ggaaatttag tggttttttc agaatatcta tttccaaaaa agaaatttct
     9721 tttttaatct tttttttcta aaaaaacggt attgaaattc cgatgtatat ttccacaaag
     9781 tttcaaatat taatttttca gactcaccgg acacttttgc atattcatta ctcatcacca
     9841 caatcatttc ctgctttgga gtcaacctac ataagttttt cattcgaagt tcaaaaggca
     9901 ttttttcatg aaaattcatc cgatttaaat cgacaagaaa aaaatcatac aagccttgct
     9961 catttttttt gataagcgta tttccagggg aatgatcttt aaattcaatc cctttttcat
    10021 gaagctgata acaaaattga gtgaattgtc ttaaaattgc ttcatgttcc ggaaaatcag
    10081 gaatttttac taactctcga aaagtcaaat cacattccaa attttcactt acataataac
    10141 tatcttttaa acccaaaaaa ctgtaattct caaaataggc aataggtgct ggagtaccta
    10201 tattattttc actcaaaaaa atagcatatt caaaagaacg ttttgcttta gattttctga
    10261 agtaacgata aattacagca ttataaaaat ttggaatttt aaatgattta atggcaacac
    10321 ttctattttg ccacgaaaaa atctttatac agtttcgtgt tccgtctgaa agtattttcc
    10381 cttcagtcgt gaagtttttt atgaaaccca gtaaaattga ttcaggaaaa tcaagctttg
    10441 aatttatttt gaaatccatt taatcatttt ttttaacact atttaatcaa ctcgaaatta
    10501 ctttgaggca actgtggcaa attgaggata gcgaatatct aattgtgtaa atagaaccgg
    10561 aatgttataa aggtaaggtt ttagcgattt gcttttatta attccttcac taaccttaac
    10621 tgtataaccg gcttctttat acattctgtt tatgcttttt tcggtaaaaa aacgtaaatg
    10681 agtaaaatcc atcacgccat aatcttcata tttccagtct ttttttatta atactctcat
    10741 aaatgtatta tggtaacgaa catttggaat agagctaatg actacaccgt ttttagataa
    10801 tttacttctt aattttttta aaaccgtata gggatctact aaatgctcta gaacatcatt
    10861 aaaataaatc gcatcaaaat aatcttcagg taaatcgtca atgaaatttt cacaaggtcc
    10921 ggcaaatgct ttatccagaa cgagttttgc tttttctgct tcttcttcca tgagttcaat
    10981 tccccaaact tctgcgttgt tttgtttttt tattacctca gcgaaacagc catttccaca
    11041 accaacttct aaaatttttt tagcttcttt tggcaaataa acaagcatct catctctaaa
    11101 attatcataa tatcccgttg gtttattttt atagtccatt ttatattttt ttcaaatgta
    11161 ttaattattc ttattttttt tataccaacc ggaaattaaa tataaagtta gtttagaaaa
    11221 ctcaatacta tttgcactat ttttgtattc aaccttcaaa aataggtttc tgataaaaat
    11281 gttattcata tgattgttag ttccatcatt ataattaatt ataatacatc aaaattaacc
    11341 cttcaagcca ttcagtctat tgaagaaaag gttgtagatt ttgaaaaaca tgaagttatt
    11401 attgttgaca atgcaagtaa taaagaagat ttttctgctt taaaaattgg cttaaaaaac
    11461 gattttaaaa tttcaattca attaattaga agtagaatta atgttgggtt tggagccgga
    11521 aatatgctgg gagttcaaaa agctgtagga gcatattatg tatttatgaa tagtgatgtt
    11581 attttgatag aagattcagt aaatataatg attgaatttt taaaaaagtc atcaaacgta
    11641 gctattgtag gctgccaagc tattgatgaa aatggaaaaa aattcaagtg ctttgattat
    11701 ggtttaagtt taacaaccga attatttagc cactcttttt tgcatctttt aaatccaaaa
    11761 aaatatcctt ccagaatgtt aactagtaag gaacccacaa atgttggagc agtacctggt
    11821 tccctttttt gttgtgttgc aaaagacttt gatgccgttg ggggttttga cacaaaccta
    11881 tttttatatt atgaagaaaa agatttagct tttaggattg ctaaaaattt aaaaaaagat
    11941 atttattctt taccctattc tcaatacatt catctaaaag gaaaaagtac cggaacatcg
    12001 caagttatta gaaatgagat gaagatagct cagttttatg taattcagaa aaacttaaat
    12061 tcattttcat atctactatt ttatttcttt caatttattg tttttctgat aaagtcgcct
    12121 ttcagtaaaa aaaatagggc ttatttaatt ttacttgtaa aagggatttc tgtagctaaa
    12181 tctattaaac acgcccaaaa aataattcaa atatgactat tcttcatgtt tctgctgtaa
    12241 aaaattgggg cggtggtgaa aaccatatag aaaatttgtg ctttgaatta aaaaaatcca
    12301 attcggagat aaataatata atactttgtg ttcggaatgg tttatttcac aaacgattat
    12361 taaaaacaga tttaaaatat gaaactgctc ccctagcctt taatctagac attcgatttg
    12421 cttttaaaat aattgcactt tgtaaaaaaa accatgtaga tgttatacat attcatgacc
    12481 cttctgcaat cgctttaaca attattgcag ataaatttta tagtttacct ccttttattt
    12541 ttagcaaaaa aacctctttt ccaattaaaa agaaaaagtt gacattgtat aaatacaatt
    12601 atccgaaaat taaaaaatat ttatgtgttt caaacgaaac caaacgagta acagctgact
    12661 caattgttga caaaagcaaa ttaatgacca tttatcatgg tacaaatatc ggaactaaaa
    12721 gtagcgaaac accctttagc ttaagagaaa aatttgcaat tcctaattca aaaaaaataa
    12781 taggcgttat tggaaatcac attcgggcaa aacattttga aacgctcata gaagttgtaa
    12841 atgagcttat taatattcaa aaaagaaaag attttcaatt tgttcaaatt ggaaactata
    12901 cagatagaac gactgattta ttgaaaaaag tgaaggaata taaactggaa aatgatatta
    12961 tttttttggg atatacgcct tatgcttcta actttattcc tcaatttgat gcccttttaa
    13021 tcacttcaca aagtgaagga attccacaag taatttttga aagtttttat catcaaaagc
    13081 cggtaatcag tacgagtgtt gggggaatac ctgaaataat tcaagatggt attaacggat
    13141 ttttagctcc tcgacatgac tttaaaaccc ttgcagaaaa aatcatttat ttatttgata
    13201 ataaagaact tatccaaaat ttaactacta tttcttataa aaatttaata gaaaatttca
    13261 caacacaaca aatggcacaa aaaaccttag aagtatataa aaccgtcagc aatggagatt
    13321 aacaaagaaa ttgaaaaagc ctaccaagtc attcaaaacg gaggcatcat cctctatccg
    13381 accgataccg tttggggaat tgggtgtgat gcaacaaatg cagaggctgt tcaaaaaatc
    13441 tacgaattaa aaaaacgcag tgaaagcaaa agcatgattg tgttgatgaa tggcgaaaaa
    13501 atgatttaca atatatttaa agatataccg gaagttgctt ggcaaattct cgatttatcg
    13561 gagaaaccca ccacattaat tttagataaa ccacgaaatg tagccccaaa tattattgct
    13621 gaagatgaca cacttgccat tcgaattgta aaagaaccct tctgttataa attaatggag
    13681 cgaatgaaaa gaccattagt ttctacttcg gcaaatatct ccggacaacc tactccaaaa
    13741 tcatttaaag aaattagtcc cgaaattatt aaaggtgtag actatgttgt aaatttgcac
    13801 cgcgaaaaaa tttgcgataa gccttccact attatcaaat taacgttgga tagccaggtg
    13861 aaaattattc gaaaataaaa taaccgcaaa ttcgcaaatt tttctcatca taaaaatgga
    13921 agacgattat ttatataaag aagaaagcta taaattagtt ggtattcttt ttgaagttca
    13981 caaaaatctt ggaaaagggt tttctgaaat cgtttataaa gatgcattag aatatgaatt
    14041 taataaaaat aatattcctt ttgttagaga aaaagaattt aaagtaaaat ataaagatac
    14101 ctatctaaaa cacaaatttt acgctgactt tgttgtgttt gatactatca cattggaaat
    14161 aaaaacagtt gagtgcttta atgttagcca ttataatcaa tgtcttaatt atttaaaaat
    14221 atctggttat caactagcac ttttggccaa ttttaattta atctctttgg aatacaaacg
    14281 cattgtagat tcaaagaaaa aataatttgc gaatttgcgg tataaaaaat atgaattaca
    14341 ccaaagccct acataacccc atcttcaaaa ttatctctca agcagcacag gaacttggtg
    14401 tggaaagcta tgttattggt ggctttgtcc gcgatttttt attaaataga gatttcaaaa
    14461 aagacatcga tattgtagca gtgggtagtg gaattgactt ggctctcaag gtttcagaat
    14521 tgcttcctaa ccatccaaaa gtgcaggttt ttaaaaccta cggaacagcc atgctccgtt
    14581 ttcaagaaat tgacattgaa tttgttggag ccagaaaaga aagttatcaa actgaaagca
    14641 gaaatccaat cgttgaaaat ggcacgttga aagatgacca agaccgacgt gattttacca
    14701 tcaatgcact agctttttct ttaaatcagg aaaattatgg tgaattggtt gaccctttta
    14761 atggaatcgt agatttagaa aatagaatta ttcgaactcc tttagattcg gatattacct
    14821 attctgatga tccactgcga atgatgcgag cgattcggtt tgccacccaa ttgaacttta
    14881 ctattgaaga aaaatcattt caggcaattt ctcgaaataa agataggatt tccattattt
    14941 ccggtgaacg gattgtagat gaattgaata aaatattatt aactgaaaaa ccttcggtgg
    15001 gattcatttt gttatatcaa accggtttac ttgatttaat cttaccggaa ttgactgctt
    15061 taaatcaagt agaagaaatg gaaggtcata cccataaaaa caacttttac cacaccttgg
    15121 aagtagtaga taatatttgt ccgaataccg aagatgtttg gctgcgttgg gcggctttgc
    15181 tacacgatat tggtaaagct cctaccaaaa agtttaacaa aaaacaaggt tggacgtttc
    15241 acggacatga atttttgggt ggtaaaatgg tcaagcaact atttgaacgc ttgcatatgc
    15301 ctttaaatca caaactgaaa tatgtttcca aattggtgat gatgagttca agaccgatag
    15361 tattatcaca agaaattgta accgattctg cggtacgacg attattgttt gatgcaggtg
    15421 aagatattga agatttaatg accttgtgtg aagctgatat tacaactaaa aaccccacta
    15481 aatttaaaaa ataccataac aattttaaaa tcgttcgcca gaaaatagtg gaagtggaag
    15541 aacgtgatca tgtgcggaat tttcaacctc caatatccgg agaagaaatt atggaaattt
    15601 tcaatttaaa accatcaaga gaaattggtg ttttaaaaga agctattaaa gaagctattt
    15661 tggaaggcga aatttcgaat gattatgaag ctgcctttaa ttttatgatt aaaagagcta
    15721 tgaaattagg cttacaacct atcgaaaaat aagcataaga ttaacttttg tttattcgta
    15781 tgatttttta aaagcaacgt atttttgcac taattataaa tgcaatgaaa actaacaaac
    15841 ctgttattat ttggttactt tccggatgta ctttattatt tataatggtt gtcgttggag
    15901 gaattacccg attaactaat tcgggattat caatgaccga ttggcatttg gttaccgata
    15961 cttttcctcc tttatcagaa gaaaaatggg gcaatgcctt cgaagaatac aagaaatttc
    16021 cggaatatca aaaaatcaac attcacaatg attttacttt agaagattat aaatttattt
    16081 acttctggga atggtttcac cgctttattg gtcgaataat tggtttggct tttatcattc
    16141 cgtttgggta ttttttgatt aagaaaaaat tagattctga aacattgaaa aaatgtatag
    16201 tccttttggg aatgggtgct tttcaaggtt ttttgggttg gtttatggta aaaagtggtt
    16261 taattgacaa cccggatgtg agtcatttcc gattagcatt acacctgact tttgcattta
    16321 tcacttttgc ctacacgcta tgggttgcct tagatttaat ttatcctgcc aaaaataaag
    16381 taattctgcc tctgagaaaa ttggcccgaa ttgcgatggt tgttttaatt atccaaatca
    16441 tttatggtgg atttgtggcc ggattaaatg ccggattaat tcataaccat tggccactga
    16501 tgagtgaagg aaaattaatt cacgaaacag tatggattga acaacctaca ctttttcaaa
    16561 acttaactga aggtaaaagt ggtgttcaat ttattcacag aactttagca tatgttgttg
    16621 ttattttaat ggttttattg tatttcagaa gtaaaaaata ttcactttct accactcaaa
    16681 tcaaaggatt aaatacgttg gtagtatttg tttttatcca atttctattg ggtattttta
    16741 ccttgttgct tcatgttcct ttatggttag ggttggtgca tcaagtaatg gctttctttt
    16801 tattgagtgc gatgacattt acattgcatc gtttcagtaa ataaaattta taattgacta
    16861 tattgtcgca gtttcgttag aattatttaa atgtctaaac ttgccgttca ggataaattt
    16921 ttagatttat cagattatgg aagatctttt ggaaaatttt ttgctttaca attaaaaaat
    16981 acctccttca cgcctattca tgtaacactc ctattcggat ttaccggaat agtagctatt
    17041 tatagtattt tagaaggttt ttattattgg gccgcctttt ttatcgttct taaatcagga
    17101 attgatgctg ccgatggaga gttagcccga ttaaaaaaaa caccttccta ttcaggtaga
    17161 tatttggaca gtatttttga tattcttttg aattttggtt ttttgactgc catttgttat
    17221 gtttcaaata ctaccgtttg gttatccttt ttagcttttt tgtctattca attacaaggt
    17281 acattataca attattacta tgtgatttta agaacaaaat cagttggagg tgatacaacg
    17341 agtaaaattt ttgaatacaa atcacccaaa gcattaccag gtgaaaagca agaagtagta
    17401 aactttttat ttaaaattta tagcattata tatggcggat ttgataaaat cattcatttt
    17461 ttagacaaag aagcctacaa agttaaaacc tttccaaact ggtttatgac tcttctttct
    17521 gcttacggat taggttttca attattaatc attgctataa tgttgccctt aggatggatt
    17581 gaatacattg tgcctttttt tattttttac accgttttta tttttttatt aattggtatt
    17641 cgaaaacaat atattgcata aaaaaatccc taaactatct gaattcaggg atgatttcta
    17701 ttgtttgaaa aatttaaaaa gcggtactca aagtaagcgt taaccctgta ctttctggaa
    17761 caaaacgaca aacaccaccc acacaaacca atccgccacg ttgtcttcca taattcatac
    17821 ctattcgagt tgaattaatt cgataagcac ctcccaaatt aaaataatga ttacgtttgt
    17881 cgggattatc atttccataa ttgataatat cccaaatata gaaggaatac gtatcattga
    17941 tgtctaactc taatgtacct cccgcccaat ttttaaaatc agcatcagcc cacatatgtt
    18001 ctgcctcaaa acgaatcgat ttagtttcgg caaaattata aatggcttca acggtaataa
    18061 tatttgtatt tacatccgaa ctggcatctt caatccatgg tttgttataa tactggttta
    18121 cataattaaa tcctgtgtgc catttttcgg aaagtttctt tttaacttct aagttataat
    18181 cagaaaaata tttggtccct actccaaaaa aattagtttc ataattgctg ggcgttaatt
    18241 gatattctcc gggtaaatta taccattgtg acatgttgaa agcaatctta gttccatatt
    18301 tcccgcctaa tttagtgtct tttgcaaact cataaaaaat atctatttgt ccacctgttt
    18361 cacccgcttt taaaatggtt ggcgacttga aatcaacacc aacctgagct tggtaaacat
    18421 aaatattggc caaatttgaa tgttgttgtt tggttaaagc cggtgtaaaa tttaaaactt
    18481 tatcgttaaa atttaaactt gtattttcac cggaaacggt tggaaccctt tcagataaaa
    18541 acaacatatt ttcggttctt ctcaaagttg catctatacc taagccctgt ttagaatagc
    18601 ctaaattaag aagaattgca ttacctggtt tgataaaatc gttatctatt gtattactaa
    18661 cacgattcaa aacagcatct tctgatttgt aattgtattc aactgacgag taaaatgaat
    18721 tgattgaata atttactcta cccgaaaaag cattagttaa agcttcaaaa tttggctcag
    18781 gtatatcttc tattgattca tatcgaccca cgtaacttaa accaaatgaa agatcagatt
    18841 tttctaattt taaggcttca ctcaactgaa aatcggaatc aaaaccataa attttaccat
    18901 ctgacacatc aaatccggaa cgttgctgac caaacaaagc ggttaatttt acattagaag
    18961 aaggtctgta aataaatctt ccaccacgca aggcagtatt aatacccaat gctctgtctt
    19021 cccaggcacg aagtaacatc ccacttccaa actgctcata gaaataacct gccgtgacat
    19081 caactttttc agttttataa tttagaaaat aagttcctaa tcctattccg ctgaattccg
    19141 gattgtaatt aagaagagct tcctttaaat aaccttctac ttgaacacca gcagtccaat
    19201 ttttatattg ataattggca aaaagataac tattggatcg aaccgggtct tccggttgaa
    19261 tggttccaat ttctttatcg ttcaaatacc attgggcatt cgattcaaat ccgccgtaaa
    19321 ctctaccgta atctttctta gtaatactat ctttttcttg agaaaacaaa attgaaaaac
    19381 tcaataaaaa acttgttaca ataatctttt taaactgcat tatttaagct ccttaagttt
    19441 attatacaat tccatttctg caccttgact gtaaccattg tgtacataaa caattttttg
    19501 gttttttacc acaattgtat aaggaacatt agctatacct aaagcccttt ttaaatcctg
    19561 attggtatcc aaaagaatag tataggtcca attcttgcca tttaataatg gttttactct
    19621 tttttgcgtt cgcgtgtcat caatggaaac agcaattaat tccatgttta attctgttga
    19681 ccatgttgta taatattcat tgattgcgtc taattcattc aaacatggtg cacaccatgt
    19741 ggcccaaaat gcaaatacgt ataatttatc tttttcatta aaatcatttt taacattaaa
    19801 agatttattg gtgtcagatg ttaaagtaac attaggcatt tgtttttggg aaaatgcatt
    19861 gatgacatta aataataaaa acgtaaacaa gataattttt ttcatgaaga aattaaattt
    19921 aaattttagt ctttttttta caatttcagt tattcttaat ttgaatactg atttttaata
    19981 attaggcaat aataaaaaaa aataacctcc aaataagcaa tacagaaaat tattttaaaa
    20041 aaaagttact aagaagttat ttttatttta aaaaaagtat atttttggca aaactataat
    20101 taccgctaaa caattaatta acaaaccact aaagaatgaa aaaaacatta ctcacagctg
    20161 ttttgctgac ctttggtttt gcaaatgctc aaacttttgt aaataccaat cctgaaaaca
    20221 aaaaagtcat tttagaagaa ttcaccggaa ttcattgtgt ttattgtcct caagggcatg
    20281 caattgccaa ctcaattaaa aatgccaatc caaacaatgt atttttaatt aatgtacatg
    20341 ttggaggtta tgctgaacct aattcagggg aacctgattt cagaacatct tttggaactg
    20401 caattgcggg tcaaactggt ttagttggat atccggctgg tacagtaaac cgtactgctt
    20461 ttgcaggatt aaatcaagga gctttaggaa ctactgctat gaatagagga aattggtcta
    20521 atgcttctaa ccaaacttta aatcaaagtt cttatgttaa cgtaggtgtt cagggtgtta
    20581 ttgatgcaca aacaagacaa ttaactgtaa ctattgaagt ttactataca ggcaacagcc
    20641 ctgtaagcac gaacaaacta aatattgcac ttttacaaaa taacacatta ggaccacaaa
    20701 caggtggtaa catgggaaat aattataacc atcaacaccg tttggttcat ttattaaccg
    20761 gtcaatgggg aactgatatc actacaacaa caaatggatc gttctacaca caaacattca
    20821 attacactat tccggcatca tacaacaaca ttccagcaga aattggtgaa atggaattag
    20881 tagcctttgt aacagagaca caacaaaaaa ttatcagtgg taatggtgca aaaccaacct
    20941 acacaggatt agctaataat gatgcaaaag taaaaagtat tgaaacaatt ggagaacaat
    21001 gtactaattc tattggtcca aaagtgacta ttcaaaataa tggacaaaca cctttaacta
    21061 gtttatctat tgattatgca attaattcag gagctactca aaactatgtt tggacaggaa
    21121 accttgctcc attgactagt gcaatagttc aattacctgc cgttagctat actcttttgc
    21181 caactaacac ggtaactata actattccaa atgatgacaa tgtttctaat aatagtggtg
    21241 taattaattt caacaaagca gttgaaacac aatttacaaa tatcactgta aaaatttcaa
    21301 ccgatcaata cggtagcgaa acaacatgga atttaaaaaa ttctgccgga acaactgtag
    21361 ctcaaggtgg tccttatact gatgcagcag gtgctggagt ttttccaaaa ccggatgtaa
    21421 atttaacttt agcaaatgac tgttatactt tttcaattat tgattcttat ggagatggaa
    21481 tgtgttgttc atacggaaat ggaagttacc aggttttagc aaatggtgta ttaatcccag
    21541 gtatgaccgg aggtaatttt ggaagcggtg aaagcaaaaa atatggcgtt aataccaatt
    21601 tgagtgtttc tgctttcgat gcaaatgcaa ttaaatttta cccaaatcca accaatggaa
    21661 tgataactat ttctctacct gaaatggcaa aagtttcaat caccgattta agtggtaaat
    21721 taatttcgac ttcaatgctt gaagctggag attctacttt aaatctaagc gatttatctt
    21781 caggaatata ccttatcaat tttacaggtg acaattttgc taaaacagac aaaatcattt
    21841 taaaataatt taattgggtg tttaatagag attcttttaa acaccccttt ttaaaatttt
    21901 aaataaacaa taataaatac gttatgaaaa aaattaccta ctcaatattc tttagttttc
    21961 ttttcttgac ttcatttgct caagttgagt ttaaaaaatc tgatggaacc attatcactg
    22021 atggaatggt tattcctttt ggaaccacgg ggacttctgc ttaccttaac ttcagagtta
    22081 aaaacgtttc aactactcca ctagacatca aaattaaatg tattggtttg attaatacaa
    22141 ccggggcaaa ttttgaatta tgctatggcg gatcttgctt tgacagtgtt actttaaacg
    22201 gtatttatcc tgattacgaa aatatcttgg ctcccggtca aagtaatcct tcgcaaggag
    22261 atcattttgt gaattttaat ccaggttcag gtggtataat ggacttcgta ttttcagtat
    22321 atgccttagg ttttgaatca agtgcaatta cttttactta ccgatatgat ccagccttaa
    22381 gcttaataaa ccttgaaaac ctatcaagtt taggtattaa tgttaaaaac acggttgttt
    22441 ttgatgaatt ttcgtttaca gcagcaaata aaggaagcgt aacattattc aatctaaacg
    22501 gtcaattaat taaagaatta aatttttctg aaggatctca aaatatcaat ctaagcaatt
    22561 taaatacttc tatttacgtt gcacgtttta taaactcaga aggagtttct tcaaatgtaa
    22621 aaatattcaa acaataatag catgaataaa tttggtgtct tagggatatt acttataatt
    22681 ttcagttttt tttcttgtag tacagattac aaaattttgg aatcggttga atctattggt
    22741 ttaactactg ataattcaat taaggtaatt ggagagacta ttacatttac tgttacaacc
    22801 aattcagggg aaattattac tgaagatgct actattcttg tgaataatac tccaattgat
    22861 ggaaatacat ttaccactac ggaaattggc acctatacag ttcaagctaa atattacagt
    22921 gtactttctg agccaatttc agtaagattt catgatggtt ctgaaattaa ttttgtaaaa
    22981 agagttttaa ttgaagatta caccggaaca tggtgtggca attgcccgag agtatcacat
    23041 gcaattgaat tagctcgagc tcaatcagaa tttattgtac ccgttggtat tcatcgttcc
    23101 agttcaaatc cagcagatgc ggcttatgac ccttataatt ttgattctag tgccttagaa
    23161 gctgttttaa acatgtcggg ttatccaaaa gctttactga atagaatgac tcgttggcag
    23221 cctttggaac aaaacaatat cactcaagta attaatttaa cacaaggtga taatccaaaa
    23281 ttaggtttat caatgtcaaa ccaagtaact tcaacaaata taactcttga tgtaaatata
    23341 aagtttagtc aagattttga aaatctgaaa ttagtggttt atgttttaga aaatggatta
    23401 atttacgatc aagtaaatta tacggctttt tacggtggtt taccttatat tactaatttt
    23461 gaacatgaac atgtattaaa atcatgtttg acacctattt tcggggagcc aattgcttca
    23521 gaagaaacta aagtaggaaa cacatacaca aaatcatttt caatttcaat tccttcaaat
    23581 atagctaata gcaataacat tgaatttgtg gcttttgtta caggtgatga taataaagct
    23641 attaatgttc gaaaagcgaa tcgcaacgaa aatcaggaat ttgaagaatt ctaattattt
    23701 aaaataaata caaaaaaaag actgtcaaaa ttgacagtct tttttttatc ccaaccattc
    23761 cttaaaatca tttacgcgtt cgcgactgac aataacctca tcttctttgt atgttggaag
    23821 aatgactttt aagcgtgaat tactataaac caaaatatct tttatcgatt ttaaaggaat
    23881 aatgaatttt ctgcttactc taaaaaattg atttggattc agttctgttt cgacatggtc
    23941 taaagtaccg tcaatcaaat aatcacgatt gtcaagggtg tgtaaataag tccctttgtt
    24001 ttcactataa atacattcca cttcttctat cggaatcatt ttgagacgtt ctccaatttt
    24061 aattgtaaat cgttgtttat atgttttttc aaccggattt actaacattc gtttaatcat
    24121 atcaaaatcc agagataaat taggtaaagc ttgatttctt gatttgtatt tgtttacagc
    24181 tatttccaaa tcatcttcgt caatgggttt taacaaataa tctatactgt ttaatttgaa
    24241 tgcccgtaaa gcatattcat caaaagcagt cgtaaagatc accgcacttt taatatcgat
    24301 ttgttcaaaa atttcaaaag acaacccatc tgataattga atatctaaaa aaatcaaatc
    24361 cggatgtgca ttattttgaa accattgcag cgactcttca acagaatgta aaagtgtgtc
    24421 tacttgcaat cccaactttt ctaatttgcg ttgcagtaat cgtgcagccg gtttttcgtc
    24481 ttcgatgatg attattctca tactattata atgattatgg gaatgggtaa aagttgaaca
    24541 ctattcccat ttactattct tgtctttatc cattaactct ttaattttac gttcttccca
    24601 attgttacct aaaaaccatt gtggtacaaa aactgaaaat ccgtgagctg ctaatccaat
    24661 tccccaaaag aaaagtgtcg tgaaattttt atactgaaaa taagattctc ccgggtctaa
    24721 atcttgataa ttcacaatga ttatcatcaa attaataatt acataaacaa tggcatgtgt
    24781 gtaaaatcct tttatttttt tcactcgttt aagagcttct tggtacttta tttcatcgtg
    24841 agcattcata attgatacgt tttttattga tatttatctt tgtttttttc ctcttccata
    24901 aattgtttaa tcttccgatt ttcccaatca ctgccaaaca tagtatcagt gccaaaaaca
    24961 cctattccgt gaaacaacac tcctattccc catcctatca ttggataaaa aaaccaaaaa
    25021 tgctcgggtg tgtatttaat attaacaaag gctataatgg gtattacaaa taagtaagca
    25081 attaagtggc tgtaaaaccc ttttatctcc tgaacctttt tactagcttt ttgatatctt
    25141 tcgtactctt ctttcgtttc catacttaca aagtttaatt ccatttattt ttttgtttgt
    25201 cttccttttc taaaatctct tgaatttttc tttcttccca ggattttcca tacccaaaag
    25261 tctccaacgc atgaaaggtt aaacccatac cccaacctaa cattgggaac caaaaccatt
    25321 gaaaattgga ggtattcaaa ttcacaataa ttaaaaccgg aataactacg caataggaaa
    25381 ttaaattccc ataaaaacct ttaagttttt ctactcgatc tttagctctt aaataagccg
    25441 tattttcatt atatttcatt tgtgtttcca taattgaaat ttgttttgtt aaaataggta
    25501 ctagtatttt aaatgatttt tctgtttctt ctaccaacac ttttcgttgg gttaaaatag
    25561 catagcggtt aacgatattt tgtaaaccaa cgccttttct gtctgatagc acttctttct
    25621 tttgcaaatt gttttcaaca accaattggt tattttcgat gtaaatttta atatgcaatg
    25681 gtttgctctc gctcactaca ttgtgcttga tacaattttc taacaataat tgtaaagata
    25741 aaggaaccac ttttgcttct tcattgttaa atccatccgg taattcgaat gtgatactgt
    25801 tttcaaaacg cattttcaac aaattcatat aggttttggc aaaagctaat tcttctgcta
    25861 ccggaactaa ttccttatct ttttgttcta aaacatagcg gtatatttta gacaaggaag
    25921 tggtaaactt ttgagcattc tcaggatttt cttcaattaa tgaactcaga acgttcaaac
    25981 tgttgaacaa aaaatgcgga tcgatttgat tttttaaact ttcaaactgt gccgatgctg
    26041 ttccggcaat cactttttgt tcttttactt tattttcctg ataggctttg taaaaataaa
    26101 aagcgtgaat tcccaaggtt acaaagaatg ttacaacgat tgccaccaag taatttgcag
    26161 gccgttccgt tgctaaaaaa gcttccaatg actttccttc aatgataaca tcttcaaaaa
    26221 ttcgcaataa aaaaatcacc aaaatggaca ctaggaacga agcaaatgct ccaaaaaaaa
    26281 ggcgtttgct tgaaaaacgt tcctctttaa aataattgtc caattgcatg aaaagaatcg
    26341 cattggcata atacaaagaa tacccataaa gcattgtaaa accaaagctc attgctaatt
    26401 caatattcca agccacatgt ccacccatca caacatttaa aagcgttaac acaacaaata
    26461 cgcctaaaag aataaaggtt gctcgaggaa attctttgat taatttattc atttttttgt
    26521 tatttacaag ctgttaatgc ttcttttgct ctttctaatc cccaatttgg gtgaaaagcg
    26581 gactcaggtt taaaagtagc aaaaagttcg attgattttg caatttgtgc acagattggt
    26641 tgcgtatccg ttccaaagaa ttttgctgaa cccatttcaa attcggcttt tccaaaaaca
    26701 acacgtggat tagttggagc aattgcctct gcttttccat acaattccat aacttttccg
    26761 gataatttca taccattggt cattgggtca aaagcaatcc aagccgtgtg aatcatcgct
    26821 tgcataacta ttaattcagc attgttgggt tgtttggtta tttctacatc caaagtagtt
    26881 tgtgcttttg tcaacaacgc attgatttgg tctttgtctt tcgttgtaaa agctgttgtt
    26941 gtgttaacca atgctacata ataattgggc aaccaattgt ctttttcagc tgaagcaatt
    27001 cgttcgaaca tggacgaagc ttccgtgttt tttccttctc cccaaagagc aaatgcttta
    27061 cccattcctt gttcgtactg accttgagag aataattgtg ctgtcacaaa taaaacgatg
    27121 gtaacgataa tttttgtcat gatgtaaaag tttaaaagtt taaaattgtt taagattgtt
    27181 taaaggttta atttgtttaa ctcttttgtt gaaacaaatt tcagttgaaa aaagagcttt
    27241 tgaaaaagag tgtgactgag ttgttgaatt ttagggatga attgttttta aggcttggga
    27301 ctaaagatta tccaactgat tcgatttctt atcttcgcta atggtccaaa agaaaccgat
    27361 aaagaaaaat cgatctgcag gttgacctat gtcacgacgt tgaaaatctc cattcaaatc
    27421 gggagtatta gcatattgat agccaaacac gttttctgtt cccaaaacat tggatacaga
    27481 gaaatacaaa atcttttgtt gcgaaattaa ataagcccaa ttgaagctta aattttgata
    27541 gggttttgtc aatccattca taaaatcagc ttcattcgga tcattgtaag gtcggccgga
    27601 attgactgtg tacgaaaatc cgatttgcga tttccaatca tttatccaat gttttgtcac
    27661 aattgacaac gtatggtcgg caacaaaact gggttgcact gccgttagat aatttctgta
    27721 aagtcgctcc gaatctataa acgaatagga aacccaatat tcggtatttt taatcgtttt
    27781 attatctctc caaaaaatgt caagtccttg ggcaaatcca tccccatttg aattgtattc
    27841 cgaatcaaat tgtggttgtt gagaattaaa agttactaaa tcattatacg atttataata
    27901 agcctctgcc cgtaaagttc gcccctcttt attgtattga taattcaaaa tgaagtggga
    27961 tgctttttct gatttgaaat ttgtttcata ctttatatat tcctgtctag gtgtttgaac
    28021 aaaatcgcca taagcaaagg aaaactgtcc attttttgaa actttatacg caaaagaagc
    28081 tcttggtgtc acctgaaatt catccaaaag ttgattatct gaagctctaa cacccaattt
    28141 taaagcaagt tttttactaa agaaaatatc cgattccgca taaaccgcag ctatcgtatt
    28201 gtcatatcct gaagaaaaag ttgtttgctc aaaaggaata aaatcttcat caaacttggt
    28261 cacgaaataa tcggctcctc cggataattt aattcgttcg gttatttttt tagaaacttt
    28321 ggctttcaaa tgtgtggcat attccgcatt ttgtactata tcggtattga ttccaatttc
    28381 attcaaactg attccataac tcacccctgt ttgcaacgac caattgtttc caaaagttcc
    28441 tttataggaa gcatttccat aaaaattatt gtttttcaaa tcaaaacgca ctttttcggg
    28501 ttggttgata tcttcctgat ttaaatcaaa atcggacaca tctaaagaag cataaatttt
    28561 taataaccca tttttaaatt ggcgacgaaa aactgtctca cccgttaacg attgatacgg
    28621 tttattccaa tcgacttcct gcggaaccaa caattgataa ggtgccaaat ttatataaga
    28681 catattgaaa gttagcgaac ttttattcca acgttgcgtg tttcccaaac ctaaacctac
    28741 tgacattaat gaaatttctg tttgttcctg aagcacatca tcattggtat ttaaggacaa
    28801 aatacttgat agtgcttcac catattcagc actataacca cccgttgaaa atgaaattcc
    28861 actaaataaa aaaggtgaaa atcggccacg agttggcaaa tcattggctg ttgcaccata
    28921 cggttgggcc acgcgtaaac catcaacaaa tgtttgggtt tcatcggctt ctccaccacg
    28981 tacgaaaagt cgattacttt cgcctaccgt ttgtgctccg ggtaatgttg ccaatgcacc
    29041 aataatatct cccacagcac cagcagttgt aacaatatcc aagggtttta aaacagatac
    29101 tctagcttta tcaccggctt caaatgtacc tgcagttaag attacggcgt cgagaacatt
    29161 cacactttct tttaatttaa tgatttggtt ttgaaattgg ctgatatcaa tttcctttgc
    29221 ataggtttca aaggatagaa aactaacaat caatgttttt gttcccgttt cttttgttgt
    29281 aaaagaaaaa tagccgtttt catctgttga accaccatca taggttcctt ctaaataaac
    29341 attggctccg ggaataggcc cgcctttttc atcggtgact ttacctgaaa ctacgttttg
    29401 actgaatgaa agaaagtaaa cgaatacaaa tgctaaagtg agacaagttt tcatgctgtt
    29461 tgattttgat gaaacaaaat tgcttcaaac caaacttttt aaaaatatta aaatactgag
    29521 ttgtaaaaaa acaaggatga attgtatctt taaacaaaaa tttaatcctt taaaattgcc
    29581 atgaatccta aaattcaaat tactaaaaca gaattactct ccgataattg gtatctattg
    29641 aacaaagtaa cctacaactt taccccggaa aacggacaac cagaaacaca acgacgagaa
    29701 gtgtatgatc gtggtaacgg agctgctatt ttattataca ataaagaaaa tcaaaccgtc
    29761 attttaaccc gacaatttcg gttacctact tttttaaacg gtaacgaaaa cggtatgatg
    29821 attgaagttt gtgccggact tttagacgaa aaaaatccgg aagactgtat caaacgcgaa
    29881 accgaagagg aaaccggtta ccgcattcaa gacgtgaaaa aagtttttga agcctacatg
    29941 tcaccaggag ctgtgactga aattttatac tttttcaccg cggaatacca accagaaatg
    30001 aaagtcagtg aaggcggcgg attagcctca gaacacgaat atatagaagt tttggaattg
    30061 gattttaatc aagcaatggc aatggtttat aatggagaaa tcaaagatgc aaagacaatt
    30121 atgttattgt tacatgcgaa aaatcttcag ttactttaaa accaaaatct tttcaacaaa
    30181 taacaattct caaatcaaaa atcgtaatta ttgtttactt ttgccttccg taaaatgcaa
    30241 tgattatgat ttataaattc cgagtgatac tcgacaccga agaagatatt ttcagagatt
    30301 tagccattga atgtaccgat actttagaag atttacacaa tgccattgta aatgcttttg
    30361 gatttgacgg aagcgaaatg gcctcttttt atacgtgtga cgataattgg aatcaggaag
    30421 atgaaatccc tctttttgat acaggagatg tgcccggtga aatgcgaatc atgagtgatt
    30481 tgactttgga agatatgctg tatgaaaagc aaaccaaaat catttatgta tatgatttct
    30541 taaacatgtg gacattctta gtggaattag ctgctattga agaaaaacaa gccggagcta
    30601 cctatcctga attattatat tcattaggtt taatgccact tgcagcaaac gacccaacat
    30661 ttgaagccga cgatgattca ctttttaccg aatttgaaga cgagctagac gaagacgatt
    30721 tagatatgtt tgaaggagac gattcttttg aggatttcgg gtttgaggaa aactggaatt
    30781 aaactgccga aaatataaat agcgaaaaac ataaattatt aaaccaagaa tccatttaac
    30841 aaattaaacg agttcagtgg agaactggag gatatgataa tttcatttat atataataaa
    30901 cgaatcaatt aaaaattcag tgtctcagca actcagcaac ttaaaaatat gattaactta
    30961 ttcaacgccc atatcgaatc actttccatc caccgtgttg gaaacaaaag ccgtaacgaa
    31021 gccatttttt tatcggagca accgtatcag accaatgatg agattgtacc tcttttaaaa
    31081 gaatttttct taaaatcttt tcgtgaaaaa gaggaaaact attatcagtt tgctcacgaa
    31141 gtagatttgg aataccacga tatgtttaaa ttggctacgg aagtttttga taatccttca
    31201 accatccacg aagtgtctaa aaaaattacc aagcatttgt ttgagcaatc taatcatcca
    31261 cacattaaaa atggggaggt ttatgtagca cattttacac atttaaccat tgacaacaac
    31321 caagtagatg cgattggtgt gtttaaaagt gaagtgcaag ctgatttttt acaatttgaa
    31381 gaaaaagcca gtaacctaga aatgcattta caacacggca tcaatttgaa taaattggat
    31441 aaaggttgtt taatttttaa ttacaaaaaa gaagaaggat ataaaatttt aacagtcgat
    31501 agcaaccgtt acgatgctcg ttattggttg gagcatttct taaatgtaga tgctttccaa
    31561 gacgaaaatt tcattactaa aaaatattta aaattttgtc aggattttgc caaagatgtt
    31621 gtttttccag ccgaagacaa aaaacaagaa gtaatgttca tgaaccgttc agtgaattat
    31681 tttgctaaaa acgatgagtt tgaagaaacc aatttcttga atgaagtcat ggaaaaccct
    31741 gatttgattc cggaattcaa aagttataaa gtagataaag gtgaaaaata cagcatcgag
    31801 gatgtaacca atttcccgat agctaacgct gccgtttctg atgctcgtaa gaaaattaaa
    31861 aacgtaatta atttggatac gaacattcaa attaaactcg atttcattaa cccggaaagt
    31921 gccgaaaaat atgtagaaaa gggttgggat gaagaaaaac aaatgtatta ttacttggtt
    31981 tatttcaata aagagcagaa atcttagtta taaaaatgtg ctaatctttc tttttataaa
    32041 acactaatcc ttaattattt atacgattaa ggattttttt tattttttgt agttataaac
    32101 cttataaaat attaattttc aacagtttat cgattttatt tggctgttta tctataatag
    32161 tgtaggttta tctcaccaca aaaaaaccta catctatgaa aacctacatc actccagagc
    32221 catctcctgg caccgttttt atttgtaccc ttattggaca caaataccaa actaccagaa
    32281 aaatcaccaa tcatttcagt gaatacaaat gcaaatgttg cggaaagcaa atgaccgatg
    32341 acggtagagg ccgattgaca ttgctaaccc ctgaacttca tgaaatcaat gaaacattaa
    32401 aaatgatttt tcataaaaaa agtcaacatc tccaaagtgc ataaatagta ggtgttggca
    32461 aaacatttct ccaattgata gtattttgag tgtttagcca acacctaatt ttaatcgata
    32521 tccaattttc caaaattata agtaacaaat tcctaactta gtcgtcctac tcctaacgat
    32581 taaatgctac tattttggaa actattctca ctattgaaaa actgaataaa cgattcggac
    32641 cggttcatgc tgttaaaaat gtctcgctag aaataaacaa aggaaatgtt tacggtattt
    32701 taggtccaaa cgggagcgga aaatcgacaa cgttaggaat cgtattaaat gttgtcaaca
    32761 aaacttccgg agaataccga tggtttggcg gtgcaatgga aacccacgaa gctttaaaaa
    32821 aagtgggagc catcattgag cgaccaaatt tttaccctta catgacagct caggaaaatt
    32881 tagaattggt ttgtaaaatc aaaggaacac cgtattctaa agtgacagaa aaattagaac
    32941 ttgtcggtct tattgataga aaagacagta aattcagaac tttctcatta ggtatgaaac
    33001 agcgtttagc tattgcttct gcccttctta acgaccctga aattttaata ttggacgaac
    33061 caactaacgg tttagatccg caaggaattc gtcaaatcag agatattatc aaacatattg
    33121 cttctcaggg aacgacaatt ttattagctt ctcacttatt agacgaagtt gaaaaagtat
    33181 gtagtcatgt attggtttta agaaaaggac aaattcttta ttccggaaaa gtcgatggta
    33241 tgattgccaa tgaaggattt ttcgaactac aagctgataa tttggaagaa ttaaaagctg
    33301 ctttaattgc tcatcccgcc atagaaaaaa tggttttaga agaaggtaaa ttagttgttt
    33361 atctcaaaca agcatttgag gcgaaagatt taaacaagta tctttttgaa aaaggaattt
    33421 atcttaatca tttagtaaaa cgcaaacata gtttggaaga acagttttta gaattaacaa
    33481 gtaagaacta aaccaattac actttacctt tcatcaatta ccatcatgac aagacttctt
    33541 caaatagaat ttcaaaaaat atggaaaaac agagccagtc ggattttaac gattgcctat
    33601 ttcgttattc tttcctttat cgctttaatt gcttcaatta aatttaaaat tggcacattt
    33661 gaattacata ttgccgatca gggaatcttt aattttcctt atatctggca ttttaacaca
    33721 tatattgctg ctattttaaa gttctttttg gctatagtca ttgtttccat gatggctaat
    33781 gaatattcat acggaacatt aaaacaaaac ctcatcgatg gattgagtaa aaaagaattt
    33841 atacaatcta agtttttaac ggttgtcgtt tttgctttag gctcaaccct tttcattttt
    33901 atcatgtcac ttttattggg attaagtttt tcttcctaca atgaaattgg tattattttc
    33961 tccgatttag attatttgtt tgcctatttt ttaaaactta ctgctttttt ctccttttgt
    34021 ttatttttag gaatcttagt caaacgttcg gcttttgcca taggattttt attcatttgg
    34081 aatattattg aaggaattat aattggttta ttacgatgga aagtttttaa agattcggat
    34141 gattaccaaa aaatcacgca attccttcct ttggaatcga tgtcaaacct aatcaaagag
    34201 ccattcagca gactttccgt catcaaaaac attgaagaaa ccattggtgg tgctgctaat
    34261 gtaaaagatt atggtgtgca ttggtactca cttttaattg ttctttgttg gattgttatt
    34321 tttgtgttaa tgtcctataa aattctcaag aaaagagatt tgtagtatct ttgcaaacgc
    34381 tatgaagttt gccaaaaaca tcttattttt tttactgctc attattgctt tccaatccgg
    34441 aaaagcacaa tatattcaaa ttgatgacaa tcaatctgct caagaattaa ttgaaaatgt
    34501 attgataaac agttcgtgtg caaatgtttc caatatcacc gtttcgggtt gggcttttac
    34561 taatggcaat agttatggtt atttcaatcg aggcactagc acttttcctt ttcaggatgg
    34621 cgtgataatt gcaaccggta gagccctttc cgctattgga ccaaacagta gtttactaag
    34681 tgaaggcccc acaaactggc ctggtgatat cgatttacaa caagccatca atgaaacaaa
    34741 tactattaat gctactgtga tagaatttga ctttttgcca tttgccaata aagtaagttt
    34801 tgactatata ttttcttccg aacaatattt gtcgaatccg aattctaatc aatgttctta
    34861 ttccgatgga tttgcctttt tattaaaaga agtaggtagc caagaccaat atacaaattt
    34921 agctgttgta cccggaacgg caattccggt taaagttcca acagttagag gagcgggaac
    34981 catttgtcca cctgcaaacg aagcttattt tgatgctttt aatggcattg aacaccctac
    35041 aaattataat ggccaaacaa aaatcttaaa agcagaaagc cttgtaacac ctggtgtttt
    35101 atatcatatt aaattagtta ttgcagatca gggcaataat ttatatgatt ccgctatttt
    35161 tttaggggga ggaagtttta cagtagaaac cgagttagga gacgatcgat tgattgcaaa
    35221 cggaaaccca ttgtgtgaaa atgaaactct tacattagat gctacgacgc caaatgctgt
    35281 tggctataaa tggtatcgaa ataacgttct tataaacaat gaaaccaatc aaacttatac
    35341 tgtaaattct tttggtgttt ataaagttga agttgttttt aatactactt gctcttcttt
    35401 tggtgaaata gtaattgaat atagttcaaa tccaattcct aatgatgtta acctgacaca
    35461 atgtgattca gacatggatg ggttagctat ttttaattta aatgatgcaa ataacagcat
    35521 tatcggaagt aatctaaatg ttggcccacc aaagtatttt ttgagtttta atgatgcttt
    35581 taccgttcaa aacacattgt cgagtataga ttcatttcaa agtacaactc ccaatcaaat
    35641 tatttatgcc cgattagaaa atacgtttgg ttgttttgga atttctgaag ttactttatc
    35701 aacttcaatt agttctgtta ttgtccctcc aatttctata tgtgatggag atactaatca
    35761 agatggaata acagtgatag atttatccat gaatatttca ccaacggtat taggttattt
    35821 cccaaccgga ttacaagcgg tttattacag ttcaactgaa aatgctcaaa cacaaacaaa
    35881 tgcgttacca aacaattata cgaatacaat tccttttgaa gaattaattt ttgcaagaat
    35941 aactgatggt gtaaactgtt ttggaatttt accaattacc ctaaaaatca atatttttac
    36001 tccaaataat tttgaaacag aagaactttc actctgtgaa aatgaaactt attttttaga
    36061 agtggaggaa atttacgaaa gttatctttg gaacacagga gaaataactc cacgaattaa
    36121 tatttcagta cctgatactt atactgttac tgttatcaat tctgatggat gtgaagctac
    36181 caaaacattt ataattatac cttccggagt ggcaacaata acttctatag atgtacaaga
    36241 tttttcggga aatcaaaata ctgtacaaat caactatacc ggtttaggag attatctctt
    36301 ttctttagat ggaaattatt ttcaaaacag tccgattttt actaatgtgg aacccggaga
    36361 atatcttgtt attgttaaag atacaaacgg ttgtggaact gttacccaac aaattttcgt
    36421 actagactac ccccgttttt ttactccaaa tggggatgga tttaatgact tttgggaaat
    36481 taaaaacttt aatcaaccaa attctaaaat ttacattttc aatcgatacg ggaaattgat
    36541 taaagaaata gttccgaatc aaaaaggttg ggacgggact ttttccggtt cgctcttacc
    36601 ttcttcagat tattggtttt ctcttttttt agagaatgga agaatcataa aaggtcactt
    36661 cgcattaaaa agataatttg ttatctttgt taccctttga aaaagaaaaa tgaaaaaaat
    36721 cgctctgttt tttctattaa ttaccctaaa ctgttttggt cagtttagta aaacacatta
    36781 tatcccacct cttggaggtt cgaccggaca accggcacaa aaccaatata tgtatatttc
    36841 ttctccaagt ttaactcctg taaatttcac tatcaattcc ttaggaggag gaacagtaag
    36901 tgggacagtt tcacgaaata gcccctatgt atataccatc ggttttggta cagattcgca
    36961 attaatggtg agcagtagtt ccatgagtgc cgttttgaac aacaaaggtt tcattgtaga
    37021 agctgaagat caagtatatg tagctgtaag actaactgct tcaccacaaa attttcaggc
    37081 aggtggcttg gtttcaaaag gattagccgc attaggtaca caatttagaa ttggagcttt
    37141 tgttaatacc ggaatttccg gaattacaaa taatcactta acgtttgttt ccatacttgc
    37201 tacagaaaat aatacattag tttctttcag cgatataaaa cccggagtta ctttaattaa
    37261 taatggtgga gccggaaata accctgcagc aataacttta aatagggggc aaagttatgt
    37321 aatggctacc actggaccta gtgttcctaa tacagacgga ctgattggag cattagtttc
    37381 atcagataaa cctattactg tgaattgtgg atcatttgca gggacaaatg gtaatgtaga
    37441 taataattta gatttgggtt ttgaccaaat tgtatccgcc gagagaaccg gaaatgagta
    37501 catttttgta cgaggttttg gtatagacat cacggaaaga ccattactta ttgctcatga
    37561 aaataatacg gaaatctttt taaatggaaa tacaggaacg ccggattata caattaatgc
    37621 cggacaatat attgccttag atggttcaaa ctacagttca gacggaaatt tatatgtaaa
    37681 tacctctaaa aatatttttg cctatcaagg agttggaggt agtacacagg ccaatcaaga
    37741 aatgtatttt gtacccccat taagttgtca aaccccaaaa attattgata atattccatt
    37801 aataaactta ataggaaata tctcttacac aactaatagt ggcgtaaata ttgttacaga
    37861 aacaggagcg aatattgatt tcatcctgaa tggcacaaat tattcactag cttcattacc
    37921 ggcttttatc aatgtacaag gaccttttaa tgtacaggga aatacaaatt acattacata
    37981 caagttgacc ggatttacgg gaaacatctc tgtttttgcc gataaacaat tgtatatttc
    38041 ttattttggg tctaatgatg ctgccactta tggtggttac tattccggat ttacatttaa
    38101 accggaaatt tcgtttaacc gtttggatgt aacgagtgat aattgtatac ctaacgtaaa
    38161 tttatccatc aattctcttt ctcctttcga tacatatcaa tggtatttta atggtgttcc
    38221 aatagcgggt gcaactaatc caaattatac gccaaccgtt cccggattat actatttatc
    38281 ggcaacagtt actacctgta atatcacgtt gatatcagat gaaataccgg ttagttcttg
    38341 tcctgaagat actgatggag atggaactta tgacaatatt gacttagatt acgacaatga
    38401 tgggattgct aattgtactg aatcgtatgg taatttagat ttaaatcttt tgaatacaac
    38461 cacaggaact attactagtg gtaattattc aaattctttt acgggattaa ttactacagc
    38521 cggaacagct ttaccaacaa caaacccttt tattggagct gttgatggta gctttataac
    38581 taatcctgct atcggtattg gaaactctgt ttcttatgaa atgaattttt ctcaaccggt
    38641 aagtttatct atggaatatg taagtaccgc aaattcaact gatttattat cctccactgg
    38701 cgagtttatt atacaatgtg ctatcaataa aacaataacc attctaaacc caaccggtca
    38761 actattaatt gataccaatt atgatggtat ttatgaaagt ggtgtgactg aatattcgtc
    38821 ttttcaaatt cgatttcgtt taaacagtac gattccatta gctgccggaa gtggaacttt
    38881 taaactcaaa tcctatttaa cagattttct atcgatcaag catatcaaca tttctgaagg
    38941 ttcttcaaaa gctactttca agattttagc cacttgtgtt cctaatgatt ctgatagtga
    39001 tggtattcct aatcaatttg atttagatag tgatgaggat ggaatatcag acaacattga
    39061 agcacaaggg cagaacttca ttgctttcaa ccctatagat gctgatggaa atggaattag
    39121 tgatgtttat ggaaatggtc tttttccaat agatacagac ggagatggag tgctaaatta
    39181 tgtagattta gacagtgata atgatggaat atatgactcc gtagaaacag caatcgacac
    39241 aaacggagat ggcatcggaa attatcttac attggacagt gacggagatc tatgtaatga
    39301 tgttattgaa gcaggattta tagacgaaaa ttcagacgga ttattaggga acgggtctat
    39361 tgtagtgaat agttttggcg ttgtcactaa tgctccaaac ggttatacgc ctccaaattt
    39421 gaattacatt attggtgcac caattgcaat tactgcacaa ccgcaaaatc aattcacttg
    39481 taatacacaa aattcttcgt ttacttttac cggaactgtt tctgatagct atcaatggca
    39541 attgagtaca aatggcggtg caacttggtc tgttatattt aattctgctg tttactcagg
    39601 agcaacaact acaaccttac aaataaccgg agcaacaact tcaatgaaca attatcgcta
    39661 tcgggttttg ttaaacagag ttggaaacag ttgtggatta ttatctgatg gagcaacatt
    39721 aacagtatat ccactacccg ttctaacatc accggtttct attgtgcaat gtgataatga
    39781 tgctacaaat gatggaattt cggaagtgag tttaattcag aaaaatgata taatttctgc
    39841 taatgcggct aatgaaattt ttacttattt cacaacacaa cttgccgctc aaactaataa
    39901 ttcggccttt caaatttcaa atcctttaac ctacaataca ggtaatacaa ctgtttgggc
    39961 tcgggtagaa aacagtgatg gttgttttga agtaggacaa atcgatgtat ttgtttctgc
    40021 tacccaaatt ccaaattcat ttattaggaa attttcaaaa tgtgacgaat attttgatgt
    40081 cgttaatgat gacagagatg gtgtctctca atttgatttc agtagcgtga cagctgaaat
    40141 cagtggtcta ttaccaacaa caactccatt cacaattaaa tattataaaa cagaagcaga
    40201 tgctttggcg gaaacagata ttaatggtaa ttcattggaa attaatccat ctaattatca
    40261 aaacattggc tttcccggat tccaacaaat ttgggttcga gtagaaagct cattagacaa
    40321 tgcctgttat ggtttaggac cttttgtgga attaacggtt gaagaacttc cggttgctaa
    40381 tccaattcct gattttattt cttgtgatga taaccaagat ggtaaattcc ctttcaatac
    40441 aagttcaatt gaaacaactc tattaggaac gcaaaatcct agtattgtaa caatcactta
    40501 ttttgatcaa aataacattt cactcccaag tccgttacct aatccttttt tgactggctc
    40561 acaaactatt agattaagag tgacaaacaa tgcaacttct gacccaaatg gaccttgtta
    40621 tgatgaaact actttaactt ttaaagttga tattcttcct gttgctaatc ctgttcctga
    40681 ttttacaact tgtgatattg atggtataga agacggcttg tttagttttg acacatcaat
    40741 aattgaaagt acactattaa atggacaaac cggaatgatt gttacctact ttgatgaaaa
    40801 taataattct ttatccagcc cgttaccaaa tccctttgtg acaaaaacgc aaactattac
    40861 tgctgtagtt acaaatccca taaatacaag ttgtccggcc actacaacta ttgattttat
    40921 tgtttatcca ctacctcaat tagcagatgg attcaaagaa attatctgtt tcggggtaga
    40981 tgcaataact tttgatgccg gtttattagt tggaaacagt aatgatttca cttatcaatg
    41041 gttcaaagat gatgttctta tgattggtga aaataactat caacttacgg taaatcaaaa
    41101 tggaatttat aaagttattg ttacttctgc atttggctgt gaacaaacaa gaacaatcga
    41161 aatagtttac tcacaaccgg caactataga tgatattgtt gtggtcgatt tggtagaaaa
    41221 taattcagtt acaattcttg tgagtggttc cggaagttat gtttacagtt tagactcggc
    41281 aaatggatta ttccaaacat caaacgtttt tgaaaacgta atagctggaa tttacaacgt
    41341 ttatgtgaaa gatttgttgg gatgtggagt agtttcacaa gaaattccgg ttttaggagc
    41401 accgaaattt ttcacaccta acggcgatgg ttataatgat acttggaatg taagaggggt
    41461 gagtgcaaaa tattatgcca ataccattat ttatgttttc gatcgatttg gaaaactaat
    41521 cactcaagtt gatccaaaag gcgaaggttg ggatggcact tttaatggtc aaaatttacc
    41581 ctctaccgat tattggtatt ctataaaatt cgaagatgga agaatggtca aaggtcattt
    41641 tgcattaaaa agatagttat gaaaaaaaca attgttctct atttattgct gtttagttca
    41701 atggtaagtt ttgctcaagt cacaaaagtg tgtgccggta atattgaaat atatcaaaac
    41761 tttgcttcta actttgtagc tcccagaaac gttagtgttt ggctcccgga aaactattac
    41821 cccaaaaaaa agtatgctgt tttgtatatg catgatggcc aaatgctttt tgattcgact
    41881 aaaacttgga ataaacagga atggggagtg gatgaatgta taactaaact tttccgacaa
    41941 ggaaaaataa aaaatgttat tgtagtaggt atttggaata cagacaatac aaggcatccc
    42001 gaatatttcc ctcaaaaacc atatgaaagt ttatccgtaa aagaaaagga aacagtttca
    42061 aattcactac aaaaaagagg aattactgta ccttttaaac cgttttcaga taattactta
    42121 aaatttattg ttgaagaact taaacctttt attgataaaa cctactctac ccgaaaagaa
    42181 aaagagaaca cgtttatagc cggatcaagt atgggcggac ttatatcaat gtatgcaatg
    42241 tgtgaatatc cagaagtttt tggcggggct gcatgtctat caacacattg gccgggaaca
    42301 tttgaaattg aaaacaatcc tattccaaat gcttttatca actacctgaa aaccaattta
    42361 cccgatagta aatcacataa attctatttt gattttggca ctggcacact tgatgcaatg
    42421 tatcctccct tgcaagcaaa ggtagatgaa gtaatgcaaa caaaaggata tacaaataat
    42481 aactggataa cacaaaaaat tgaaggtgca gaacattccg aaaatgcatg gagagtacgt
    42541 ttacacattc cttttgaatt tttacttaaa aaatagtttt tcataataga caaacccatt
    42601 ataggacgct ttttagcgtc cttttattat tctaaggaga aagttattaa ttatttatgt
    42661 tgaaaaaata atgtttcgtt tctaaaaagt aaaaagtaaa atctttactt tagttctagc
    42721 taaaaatgaa caaccctttt atccatgaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42841 nnnnnnnnnn gtaaaatctt tactttagtt ctagctaaaa atgaacaacc cttttatcca
    42901 tgaaaaaata tttttctttt aaattctcat ttctattttc tactcttttg ctcattttaa
    42961 tcagtgaatc aggatttgct cagttagcgg ttccctttaa agtgagatat caatctcatg
    43021 taaaaggaga catgacaatc attgcaaata caattgtaaa tagaacagaa tcaaaaaatg
    43081 ctacatccga accatataat gatatttcag aaaaagcaaa attgaatgat gaattcgaaa
    43141 tggcttatat tgatatagat aacgatgaaa ctacttttag ttccagttca gcccaattaa
    43201 acctaactac ttccactacc aaaaaagtta tttatgccgg tttatattgg tcagcattat
    43261 ataaatatga atccggtatc agagacaaaa aatggaacta cattgctgtt gataacaaaa
    43321 gaacatccgt caatgaaatc aaatttaaat tacctggaaa atcagactat atagatattg
    43381 ctggagaagt tttatttgac ggtttagaag taaaaaactt caaagaaagt gctccttatg
    43441 ccgcttatgc tgatgtaacc cgttatattc aatcattaga aaatcctaac ggtgattata
    43501 ccgttgcaaa tattaaagct acccaaggta tcatcagcgg cggtgtggct ggtggctggt
    43561 cattatttat tgttttcgaa gacgatagtg aaacagaaaa attcatcact tcctttgatg
    43621 gatttgttgg aattacaacc cgacctgttg aaatttcatt tactggattc agcactttgc
    43681 ccgaaggaaa agtaaatgct aaactagctt gtgccgcttt agagggcgac cgaaacttaa
    43741 ccggtgacca attagaaata aaatccggtg ataatttaaa atacagttta cttagtcatc
    43801 ctttaaaacc cgccgataat ttttttaaca gcactatcat aattaatgat gattatttcg
    43861 aattaagaaa cccgaatagt ttgaacacat taggttatga tacttgttta ttacaaatta
    43921 acaatcaaaa caacgctatt attcaaaaca acagcacaaa agtatcgtta aaattaaaat
    43981 catcaggcga ccgctattcc atgtttttta gtgcttttaa tgttgaagtt agtgatccac
    44041 ttaaaaaaat tgattcggcc gagttagttt caacagaaaa aaaacagatt gaattaacta
    44101 ataatataga aattaatcct aaaactgaac caacaactgt tgctactcca attgttgaag
    44161 aaacaaaagc aactgacaga ccgcaaagaa catattacag cattatggat atgattcggg
    44221 aaagtgaaaa aatgaattct gaaacagtta ctgcaaatgg taaaaagaga atcactgttt
    44281 taccaggagc tgatgttttg attccaagac agcaaaaagg atattatgtt attgccaatg
    44341 tatttgctaa accaacaaat acaactcgtt ttatttcttt attaaaaaat aaaggattag
    44401 aacctgaaat tttatttaat ccttcaacta atttacaaag tgtatattta gccagattca
    44461 ataattggga agatgcctta tcattctact attcaaatgc agatggaaaa tattttgatg
    44521 atttatggat tatgttggtc aacacatcaa ccgggacaat tttagaacct taattaaaaa
    44581 caaaaaccat caactttcgc tgatggtttt ttgtttattt taaaatctaa cttatctata
    44641 ttttaaatct ttttctatcc gtttcgttca aataaatttt tctcaaacga atagattttg
    44701 gagttacctc aacatattca tctttttgaa tgtattctaa tgcttcttct aatgagaaga
    44761 taatcggagg gataatcctt gctttttcat cattccctga cgaacgaacg ttggactgtt
    44821 ttttctcttt agttacgttt acactcatat catcaccacg agagttttct ccaattacct
    44881 gaccttcgta aatttcggta ttgggttcaa caaaaaactt gccacgatct tgcaatttat
    44941 caatagagta aggaatagct tttccttttt ccatagaaat taatgatcct ttgttacgtc
    45001 cggcaatttc tcctttgtaa ggttcgtatc caatgaaacg gtgagccata attgcttctc
    45061 cagctgtagc tgttaataac tggttacgca atccaataat tccacgtgat ggaatattaa
    45121 attttacaat catacgttca cctttagtct ccatactcag catttcacct ttacgcatag
    45181 taacgaactc aaccgctcta cctgaaagag tttccggtaa atcaattgtt aattcctcaa
    45241 ttggctcaca tttttgacca tcaatttctt tgatgataac ttgtggttga ccaatttgca
    45301 attcataccc ctctcttctc attgtttcaa taagaacaga taagtgaaga acgccacgac
    45361 cataaaccat aaacttatca gctgaatcgg tttcacctaa cttcatcgct aagtttttct
    45421 ctaactcttt tgtcaaacga tctctaatat gacgagaagt tacaaactta ccttctttac
    45481 caaagaaagg agagtcatta attgtaaaca acatactcat tgtaggctca tcgattgcaa
    45541 tagtttgcaa cgcttccggg ttttcaaaat cagcaatagt atcaccaatt tcaaatcctt
    45601 caacaccaac aatcgcacaa atatctccgg caataacttc tgttactttt ttacgaccaa
    45661 gaccttcaaa agtgtgtatt tctttaattc tagatttaat tactttacca tctcttttta
    45721 ccaaagaaat tggcattcct tctttcaaaa cacctctttc taaacgacca atagcgatac
    45781 gtcccgtaaa agaagagaaa tctaaagaag taattaacat ttgaggagtt ccttctgata
    45841 ctttaggagc tggtacattt tcaacaacca tatccaataa tgcttcaaca ttatc
//
LOCUS       DAOTCW010000003        43181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_421_length_43181_cov_43.526040, whole genome shotgun sequence.
ACCESSION   DAOTCW010000003 DAOTCW010000000
VERSION     DAOTCW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 43181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43181
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_421_length_43181_cov_43.526040"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2608
                     /locus_tag="P5335_00410"
     CDS             <1..2608
                     /locus_tag="P5335_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ73371.1"
                     /translation="NNDGIIDGSTDVDKDGIRDAFDTNSAKIGSPRDLIRKLLLDFDG
                     RNDYAEATGVLGGLTNATLMAWVNLAPGFSSTGVVVGQNNFQLRITDTRVITASCNGT
                     SVDYSAIDPISSSQWFHIATTYDGAHLKLFINGKEMNSINKTGTINADATPLTIGRLP
                     GNNSLYFKGKIDEVRVFNVALTDQQLQRMVYQEIQNNSGQVRGTVVPRDIESLPFTNL
                     LRYYRMDTYKDDIIDDLSTPTVDTGTGMKMYNHKNIYAQQAPMPFVTERTGDFATAVN
                     SPTNEVRGMDIVDFNYAIVQVKHNITETSNQTNLGMFVDAGVTITMNNDTKIQNNWYV
                     NLNGKIDLVDRSQFVQTATSVLDPTSSGSLERDQQGQSNKFNYNYWSSPVSAINSSTI
                     NHGYTVAGVMKDGTNPSSPMNLAWVSGINNPLTSNPITLSSYWIFKFQNLTPVYANWA
                     SVGQNGLLLAGQGYTLKGSGAASASQNFTFIGKPNNGQIISTVSADNLNLSGNPYPSA
                     LDADKFIQDNLATINGTLYFWEHFNTNNTHTLAGYQGGYAARTLVGGTAPVSPSGISG
                     LGSSTRTPGRFIPVGQGFFVTGNATGGDIVFDNSQRLFIKESNVDSNILFRTNQNASS
                     PNLGTGNNEEDPYTENAFMKFRIGFNSYNNFHRQLLIGFMDDLATESVDPGYDAIHID
                     NQQNDMYFLNNGTKLIIQGASHFETNKVYPLGIKTYAAGDIQFTLDGLENVEPTQPIY
                     IHDIVTGLYHDIRNEHFTINLPQGEIHNRFELTFQNHALSASDFELENQIAVAYTNAN
                     NLLTIKNTMNDNTVETVSLFTILGQTLASWNVENQTQNNISIPMSNVSTGTYIVRVQT
                     TQGTISKKIIIK"
     gene            complement(2718..3887)
                     /locus_tag="P5335_00415"
     CDS             complement(2718..3887)
                     /locus_tag="P5335_00415"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013071957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRZ73372.1"
                     /translation="MNLLVINAGSSSLKFQLIEMPLGTVISSGLVERIGLEHTKFHYT
                     IQSEEFSEAVEFSNHKEALEAIAQKILSKSSGRTIDTIVHRVVHGGEQFSKTTIITKE
                     VKDKIRELCALAPLHNPANLACIEVAEVVFPKATQVAVFDTAFHQTIPEKAYRYSIPN
                     EFYDKHKIRQYGFHGTSHKYVSKKAIEFLRLKHSKIITVHLGNGCSITAVKDGESIEH
                     SMGYGPTNGLIMGTRSGDVDQAIIFYLMDTLNYSSSQVQDLLQKESGLKGLTGFSDLR
                     DIQNEAQKGNKECLLALQMMAYRVQKFIGSYIAALNGLDVLVFTAGIGENSDVMRSLI
                     CQHMDYFGIQLDEEKNKLRSKNIRSISLEGSKVPILVIPTNEEFEMAEQAYGLLT"
     gene            complement(3897..5987)
                     /gene="pta"
                     /locus_tag="P5335_00420"
     CDS             complement(3897..5987)
                     /gene="pta"
                     /locus_tag="P5335_00420"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRZ73373.1"
                     /translation="MNKGLYIATIEPRSGKSLLSLGLMDALLSRMAKVAYYRPIINEG
                     KKSKVDNHLQLILQTFNLKVDYENAYSYTKQQMIQLVNLGKKGEVIDTIMNDYKKLEE
                     EFDFVLVEGSDFEDEVSVFEFEINLQIAKNLGLPVLINASGDNSIEDAIQNLELAYKS
                     FLGKEVGVMAIVINKIQEKNRSKFIEGIQKFCADEVQILAIPVNELLSNPSVREVVEA
                     LNGKVLFGEHLLEGIVGSFSVGAMQLHNYLTHIGQNNLVITPGDRADIIIGVLQAHKS
                     ASYPRMVGIILTGGLIPEESILRLIEGLDDIIPIISVEENTFKAANKIGRINCKIRPG
                     DTTKIQLSLETFYKYVDSDLLIENVTNFTSKGITPKMFQYNLLKKARKVRKHIVLPES
                     EDLRILEATARLIAADVVDITLLGKKETIEKSLIKSGILLPLDKVSIIDPVNSAYYAD
                     FVETFYELRKHKNVNHDIAKDLMSDVSYFGTMMVFKGLADGMVSGAIHTTQHTIRPAL
                     QIIKTKKGISVVSSVFFMCLEDRISVFGDCAINPNPTAEELAQIAISSAESSQAFGIE
                     PKIAMLSYSSGSSGMGEDVERVRKATELVKTLRPDLQIEGPIQYDAAVDMAVGKSKMP
                     DSLVAGQANVLIFPDLNTGNNTYKAVQRETGAVAIGPMLQGLNKPVNDLSRGCTVEDV
                     YNTIIITAIQAQDI"
     gene            complement(5984..>7017)
                     /gene="maeA"
                     /locus_tag="P5335_00425"
     CDS             complement(5984..>7017)
                     /gene="maeA"
                     /locus_tag="P5335_00425"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HRZ73374.1"
                     /translation="IRGPEFDAFVAAFVEAMNEVFPKACIQWEDFTGVDAIRILETYR
                     DKTCTFNDDIQGTAAIATAGFISISRLMNKSFKDQRFLFLGAGAAAFGIADMLAKKFQ
                     RDGLSEEEAYQQIWMFDVNGLLVKSRTDLAPHQLAFAHDFDACTNFAEAVLKIKPTAI
                     VGVSTVGGAFNQQVIENMSLVNERPIIFPYSNPTSHSECTAEQAYTWSKGKAIFASGS
                     PFAPVNYEGKIYTPGQGNNVFIFPALGMAIFATEARRVTDDMLLTASEAVAEQVTAAD
                     FENGLIYPQVNNILKVSINVAVRVAEHIFDSGLAGVSKPENIRTFIESKMYIPEYNQS
                     IPHNQNNNL"
     assembly_gap    7018..7117
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7570..8937)
                     /gene="hisS"
                     /locus_tag="P5335_00430"
     CDS             complement(7570..8937)
                     /gene="hisS"
                     /locus_tag="P5335_00430"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRZ73375.1"
                     /translation="MAQKPSIPKGTRDFSPTEVAKRNYISSIMKHHFEVFGFQPIETP
                     SFENSETLMGKYGEEGDRLIFKILNSGDYLDKVGSDELNVIDYKKLTGKISEKALRYD
                     LTVPFARYVVQHQNEIEFPFKRYQIQPVWRADRPQKGRFREFYQCDADVVGSTSLWQE
                     VELVQLYDAVFTDLGLGGTTIKINNRKILSGIAEVIGAKDKLIDFTVALDKLDKIGEE
                     GVKKEMLEKGISASAIEKVQPLFQFTGNVIQKIEKLNQLLAASVEGLKGVEELRFICE
                     TVEKLGLQTALIDLDVTLARGLNYYTGAIFEVVAPQVAMGSIGGGGRYDDLTGIFGLK
                     NMSGVGISFGLDRIYLVLEELGLFPDTVSATTKALFINYGNHESLYAMEAISKLRRAG
                     IKVELYPDAAKIAKQFQYADKRGIPFAVIAGQSELERMEFGLKNLQTGEQVKLNFESL
                     KKALS"
     gene            9058..10305
                     /locus_tag="P5335_00435"
     CDS             9058..10305
                     /locus_tag="P5335_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRZ73376.1"
                     /translation="MARLVKENIKIALGSIKTQLLRTILTIVIIAIGITALVSILTVV
                     SALENTLSTNFASMGANTFTIRQYELEVRVGNGRRQEREIINPIISYPEAKAFQEKYN
                     YPFTHASLSFTATSAAEVKFENKKTDPEITVVGVDDYFLTNSGLELADGRNFTGFDIE
                     NNNFVCVVGSDFENGLLKDVNPIDKIISIRGAKFKVIGILKEKGSTFGNSQDLRVMIP
                     IQVARSLFTQPNINYNLSVLVDKKELLDAAIDNAIITMRKVRKQNPVQDNNFGIVRSD
                     ELINQILSLTSVLGISAWIIGVITIFGSSIALMNIMLVSVTERTREIGVRKALGAKKS
                     TIAFQFFTETIVIGQLGGILGIILGISIGSAFAALLEFDFIIPWMAIIAAVITSFLVA
                     VVSGLYPAIKAAKLDPIEALRYE"
     gene            complement(10321..11172)
                     /gene="prmC"
                     /locus_tag="P5335_00440"
     CDS             complement(10321..11172)
                     /gene="prmC"
                     /locus_tag="P5335_00440"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006261775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRZ73377.1"
                     /translation="MEVKKYKALFQNELSPFFDAMEIDQFFYLTLEKRYQLKRVDLAL
                     NPNFEITVEEEQYWNSVLIQLKTQKPIQYILGETSFYGLTFLVNEYTLIPRPETEELV
                     QWILETNPTSSEIALLDIGTGTGCIPITLKKNLPKATVFASDVSEKALEVATKNASMN
                     QVDVTFLQNDILATTRLEQQFDVIVSNPPYVRELEKKEINTNVLQFEPHLALFVADND
                     ALLFYRKIAQLAQEYLQPNGQLFFEINQYLGTEMIALVASYGFTEIELRQDIYGNDRM
                     LRAVKSF"
     gene            complement(11182..11661)
                     /locus_tag="P5335_00445"
     CDS             complement(11182..11661)
                     /locus_tag="P5335_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRZ73378.1"
                     /translation="MIIIRPILNTDNQAIAAVIRSVLIEHNVPKVGTAYADVSLDSMF
                     ETYNIPGSSYFVVENNGKIIGGAGVSQLANEAETICELQKMYFLPEARGLGLGSQMME
                     KCLQAAVSFKYEKCYLETMPYMENAQKLYKKFGFQYIDAPMGCTGHTSCPVWMLKDL"
     gene            11742..12782
                     /gene="ribD"
                     /locus_tag="P5335_00450"
     CDS             11742..12782
                     /gene="ribD"
                     /locus_tag="P5335_00450"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRZ73379.1"
                     /translation="MNHEIYIQRCLELAQNGLGSTYPNPMVGSVIVHRGKIIGEGWHQ
                     KAGEPHAEVNAINSVKNKSLLTKSTIYVSLEPCSHFGKTPPCSDLIIQHKIPTVIIGT
                     VDPNDKVAGKGIEKLQKAGIKVKVGVLEKECRELNKRFFTFHTKKRPYIILKWAESAD
                     GFLSPLNKKEQKPVWITNVYARQLVHKWRTEEQAILVGTKTVLDDNPQLNARDWFGKN
                     PIRIILDRSGKISEDYAVKKGSQKSIILTEQEILTKDENCIYENLIFGEQLPEKISNL
                     LYQYQIQSVIVEGGRQTLQSFIDDDLWDEARIFKGNIILHQGTKAPILKGEIVVKQKS
                     IKDELIIIKSHD"
     gene            12775..13383
                     /locus_tag="P5335_00455"
     CDS             12775..13383
                     /locus_tag="P5335_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRZ73380.1"
                     /translation="MINTIIFDFGNIFIDLDKAAVDKAFRDLGLTEWHADLDELNKRF
                     EVGKATELEFIQGFQKHLPNASIEDIRKAWNAVLGDFPLKRLEFLQSLKGKYRMFLLT
                     NTDMIHIDRFEHKEGMSITRDFYNCFEKVYYSYEMGIRKPNVEVFKFLLHKHELSPKR
                     TLFIDDRKENTDAAASLGIQVWNLQVGEEEVTDLFAKKIISL"
     gene            13380..14240
                     /locus_tag="P5335_00460"
     CDS             13380..14240
                     /locus_tag="P5335_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRZ73381.1"
                     /translation="MIYIVLSVLFNAVLFVILKLFSRYNINTLQALVVNYFTAFVMGL
                     ILSDQSFNLSEIVHKPWYPGSFMLGFLFISVFYVTAVTSQRNGLSVASVASKMSVIIP
                     ISFGVWLFGEELGIAKILGIILALIAVYFTAKKEEGAVADSKNLIFPTLVFFGAGAID
                     TSLKVLQNDYLPTEEISLFSSHTFLMAFTIGVFLIGIAIIKNKMKITGKSILGGIVLG
                     IPNYFSLYYLIKMLDSEVFESSTIFTIHNVAIVMVSTLVGIVFFKEKISIRNGIGIAL
                     ALTAIFLVTS"
     gene            14247..14861
                     /locus_tag="P5335_00465"
     CDS             14247..14861
                     /locus_tag="P5335_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRZ73382.1"
                     /translation="MADSKDTYKTILTSSEEVLFKEKNSKFFGYAFPVANEEEVKIQL
                     EHLKKQHFSARHWCYAYQLGTEKISFRANDDGEPNNSAGMPIYGQIQSFEVTNVLVVV
                     VRYFGGVKLGVGGLISAYKTTAQMALENAEIIEKTIDLYYTIVFDYKNMNKIMRIIKE
                     KNLEIVSQKMELNCEIKIATRKKNAEMIFDIFSSLFEVSIKKEN"
     gene            complement(14858..15274)
                     /locus_tag="P5335_00470"
     CDS             complement(14858..15274)
                     /locus_tag="P5335_00470"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRZ73383.1"
                     /translation="MKEHQLQVRVRYSETDQMGVVYHGNYLPYFEIGRVEWLRNHGIS
                     YKSMEESGVALPIVNINISYKKPARYDDLLTVITKYKKQSSVKIEFECKIQSEKGELL
                     TTADFILVFVDLKTGKPMLPPSNIKSIIDSMELLED"
     gene            15472..16899
                     /gene="dnaA"
                     /locus_tag="P5335_00475"
     CDS             15472..16899
                     /gene="dnaA"
                     /locus_tag="P5335_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRZ73384.1"
                     /translation="MSKTAESVWENCLSFIKDNIQEQAYKTWFEPIRSVELTDNALYI
                     QVPSKFFYEWLEEHYVKLLKVALTKELGKNAKLLYKIKMENTYGNKQPFTEQLPSAHR
                     SPVKPQDVDAPLKNISPELKNPFVIPGIRNLKIESQLNANYSFDNFLEGDSNRLARSA
                     GMAVANKPGGTSFNPLLIFGGVGLGKTHLAHAIGVEIKDKYPDKTVLYISAEVFTQQY
                     IDSVKKNTRNDFIHFYQLIDVLIIDDVQFLSGKSGTQDVFFHIFNFLHQNGKQVILTS
                     DKAPVDMQDIEQRLLSRFKWGLSAELHQPDYETRISILKNILYRDGVEIPEEIIEYVA
                     RNIKSNVRELEGAIISLIAQSSFNKKEVTLDLAKNVVEKFVKNVKREISIDYIQKVVS
                     DYFQLDLDTLQSKTRKRHVVQARQLAMFFAKKFTKASLANIGSQIGDRDHATVLHACK
                     TVDNLVTTDKQFRKFVDDIHKKLSL"
     gene            16959..17432
                     /locus_tag="P5335_00480"
     CDS             16959..17432
                     /locus_tag="P5335_00480"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRZ73385.1"
                     /translation="MPVKILMVCLGNICRSPLAEGILASKLPKEKFFVDSAGTGHWHV
                     GNPPDKRSILTAEKNGINISNQRGKLFKPSFFKEYDHIYVMDTSNYADVISMAPDEEA
                     KQKVMLILDELFPGENVDVPDPYHGIQSNFDQVFEMLEEACDTIATKMLEKYSEE"
     gene            17429..18151
                     /locus_tag="P5335_00485"
     CDS             17429..18151
                     /locus_tag="P5335_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRZ73386.1"
                     /translation="MKPISFIGKLYLIPTTLGDISPDEVLPQTVKRAIDFINDYVVEN
                     EKTARKFIKSINAEKVQANLRLHVLNKFTEVSEYNEMLKPCLEGRNVGLMSEAGCPGV
                     ADPGAVIVKLAHEKGIQVVPLVGPSSILLALMGSGMNGQSFAFNGYLPIDKSEKKSAL
                     KNLERLSMEKNQAQIFIETPYRNNKLLEDILQSLQGSTRLCVATDITLPTEYIKTLTI
                     NDWKKTKVDLHNRPTIFIIQKS"
     gene            18312..19583
                     /locus_tag="P5335_00490"
     CDS             18312..19583
                     /locus_tag="P5335_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRZ73387.1"
                     /translation="MEDYFNKLKKYNFWEGNVPSLGFERKTYSDAILDYTGNKLIKVL
                     TGQRRVGKSYLLRQIADKIIKQGISAENIVYLNKEFTDFDFIGNYKELEKLIEIYRNK
                     INPKGKIYLFIDEVQNIEGWEHFVNSYSQNYVDSYEIFISGSNSKMLSGELATLLSGR
                     YVSFEIFPFGFSEYAQVLEKEKNKQTYIEYMESGGLPELFELPNEQTKRNYVSAIKDS
                     VLLRDIIQRYNIKDPRLLEDIFVYLVNNASNLISINNIVNFFKSNNRKTTYDTVSNYI
                     EYITDTFLIHKAERYDVKGKDIILGNCKYYINDLAFKNYLYQGFGYGIGYKLENLIYL
                     ELRRSGYEVFTGTIKNREIDFVAKKANRIIYLQVTYLLENEQTATREYAPLKAIPDNY
                     EKVVVSLDDVSLPPNEGILHVQTWKLADFLK"
     gene            complement(19724..20362)
                     /locus_tag="P5335_00495"
     CDS             complement(19724..20362)
                     /locus_tag="P5335_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HRZ73388.1"
                     /translation="MSKKWYFYSSIALIVALISSGFKPFEIEKMNWFYVDHAIEGLNY
                     TVPTQEELQYVNLNIPQTGKTFTGFKQALSFKESQGRYNLVNTLGYMGKYQFGVGTLR
                     TLGITDSAAFMNSPHLQEKAFKALLAINKHVLKNEIEKYKGKTINGVIITESGLLAAA
                     HLGGSGSVKKFLKSNGKRALKDGYGTSLKSYIKQFGGYDTSHIVANGKAKVK"
     gene            complement(20460..21359)
                     /locus_tag="P5335_00500"
     CDS             complement(20460..21359)
                     /locus_tag="P5335_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HRZ73389.1"
                     /translation="MKVKFSIVVFLFAVFFSNIVFSQSGFDTFFTPSDTLHISRRNTV
                     IITESVLTATALVGLNELWYADYPKSDFHYANDNANWLQMDKLGHVYSAYQLGRFSAE
                     MLAWSGVSKKNQLLYGAGYGFVFLTAVEVFDGYSSEWGFSWGDVAANATGTALYVSQS
                     LLWNEQRIIPKFSFHTTAYADQRPDVLGSSLSEQILKDYNGQTYWLSVNLHSFLKQTP
                     IPSWLNVALGYGAEGMITAIPAENDERFRQFYLSLDVNLSKIKTKSPFLSTLFSIFNS
                     VKIPAPTFEINTSGGTRFYLFYF"
     gene            complement(21361..22401)
                     /gene="mltG"
                     /locus_tag="P5335_00505"
     CDS             complement(21361..22401)
                     /gene="mltG"
                     /locus_tag="P5335_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRZ73390.1"
                     /translation="MKLKKIILYISLAVLAVVAYFGFKVYQIIFTPNTQFSEEKVAVY
                     IPTDATFEEVQTILSPYIKDMGTFVSLAERRSYSNNIKAGKFEITQGMNNNKIITALR
                     LNLPVKVTFNNQERLEDFAGRLGSQLELDSLEVLNLFKDPAFLKENGFTEETVFTLFL
                     PNTYEFYWNTSTEKLRNQFTKEYYRFWNEERKAMAALQGLTPIEVSILASIVQKETAK
                     VSERPRVAQVYLNRLKKGMLLQADPTVIYAYKKETDDFTQVIKRVVNKHLITDSPYNT
                     YKYGGLPPGPIFMPDVNAVEAVLNPEQHDFLYFCASVTNFGYHEFATTLEQHNVNARK
                     YIAWLTNQTAPQ"
     gene            complement(22430..22954)
                     /locus_tag="P5335_00510"
     CDS             complement(22430..22954)
                     /locus_tag="P5335_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRZ73391.1"
                     /translation="MNSLQGQHIYLRALEPEDLEFVYALENDTSVWEISQTQTPYSKF
                     LIRQYLDNAHQDIYEAKQLRLAICKNDSFEAIGLIDLYDYDPKNQRAGIGILIQNPEN
                     RYQGYGHQALELLIHYAFEHLFVHQLFATIATTNQASISLFTTFGFHCVGIKKDWNFN
                     HGIFSDEALYQLIR"
     gene            complement(22957..23748)
                     /gene="dapF"
                     /locus_tag="P5335_00515"
     CDS             complement(22957..23748)
                     /gene="dapF"
                     /locus_tag="P5335_00515"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRZ73392.1"
                     /translation="MKIQFFKYQGTGNDFIMVDNRLENFPKKQTNLIEKLCDRRFGIG
                     ADGLILLENDQNADFRMVYYNSDGNESSMCGNGGRCIVAFAEQLGIIQQETTFIAVDG
                     EHFAKFTTAGLVSLQMKDVTTIQVASDYVFMNTGSPHHVTLVDDVKSVPVKELGSQIR
                     YSDLYGQAGSNVNFVEPLSETLFAVRTYERGVEDETLSCGTGVTAVAIAMNVIGKTTS
                     NDISLLVEGGSLQVRFEKEDRNYTHVFLTGPATFVFSGEIDLATL"
     gene            23884..25272
                     /locus_tag="P5335_00520"
     CDS             23884..25272
                     /locus_tag="P5335_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRZ73393.1"
                     /translation="MKNATNLFLVSLLSGITTIGAYKLFIEDTTSSKNIITTANALSA
                     KTVGLSPETIDFTEAAETALHQVVHVKNVSYRTVSNPIMEFMYGYKGGQQQQQIGTGS
                     GVIISEDGYIVTNNHVINEAQDIEITLNNKKTYKAKLIGTDSKMDIALLKVDAEEKLP
                     YAVFADSDQVKVGEWVLAVGNPYNLNSTVTAGIVSAKARNLDTGGIQSFIQTDAVVNP
                     GNSGGALVNTQGELIGINTMISSNTGSYVGYSFAVPSNITRKIIEDIMEFGNVQRGIL
                     GIEGYELNGNASKELNLSETQGFYVNKVTKNSGAEKAGIKTGDVITKLDNQNITSFAE
                     LSGYVNTKRPNDEIKVAVNREGKSLIIPVKLTKKELINYEFYGLEIEDLDAADKKQFG
                     IKSGVKIKNITNEKLVPYSEELKGSIILSIDGIKATDVESISRNLGSKSEKQGVRVEL
                     MLKNGQVMRIII"
     gene            25425..26876
                     /locus_tag="P5335_00525"
     CDS             25425..26876
                     /locus_tag="P5335_00525"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRZ73394.1"
                     /translation="MKSNPSLYEKELSFQADRRRAGVEFIKIISDLWYDKSIEVVLFR
                     NQLIDKNVSEILNLHEYAGEFVGKPISIFDSVEIAREMLSLDLPPSKLDIGKLTFEYH
                     LEDDKYHNTKSFVIDKLRKAKDVDSIKPKDVVLYGFGRIGRLLARELMSKTGKGNQLR
                     LRAIVTRDKNDATILEKRASLLRYDSVHGDFQGSVSADVENNALIINGTTVHIITANG
                     PEEIDYTKYGIKDALVIDNTGAFTNEEALKRHLVSKGVSKVLLTAPGKGVPNIVYGVN
                     HNDHNPDKIDIFSAASCTTNAITPILKAVEDTLGVVKGHLETIHAYTNDQNLVDNMHK
                     KYRRGRAAALNMVITETGAGSAVAKALPSLAGKLTSNAIRVPVPNGSLVVLNLEVGKE
                     TSIAEVNEIMKKYALEGELVEQIKYSLSNELVSSDIVGTNAPSIYDSNATIVSGDGKN
                     IVLYVWYDNEFGYSHQVIRLAKYIAKVRRYTYY"
     gene            26958..27680
                     /locus_tag="P5335_00530"
     CDS             26958..27680
                     /locus_tag="P5335_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73395.1"
                     /translation="MNYFYLCFLLILPCSGICQIQPTPKSYHKIKFLYQKKSTLRLDE
                     KGVYADTLQLKKMFPDVEFEKIKLSEENNNTVVGFVPIEKLNGIHKNEILNHLSSNNC
                     LIYGTAYPKKDLIDFTVDYNSPETALAYYLFGKTQTQFSHTYSYTYLEKNARLEISSS
                     GTKQAVAITKNQLTWINKDQLTGTFSVTEKPNTKKNQLIQIEKNLPRWISPIPFFENV
                     QMGIKSIYDVEYDYLLITVSYE"
     gene            27779..28177
                     /locus_tag="P5335_00535"
     CDS             27779..28177
                     /locus_tag="P5335_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73396.1"
                     /translation="MKNLLLLVIFTTHLSFAQDKVSTYKFGQNGMELIARSPSGTVII
                     STFNAKMTIRQDIARKIYDLYKDHKISGNTRLTVYGKEANVMGKCYIKTKNNLTSVEF
                     YYEKVSWNNGLVEVYENIKTSHEVVAMKTN"
     gene            complement(28313..29428)
                     /locus_tag="P5335_00540"
     CDS             complement(28313..29428)
                     /locus_tag="P5335_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HRZ73397.1"
                     /translation="MDYFWQQTDFPNFQFKVDDFISQIQEFATEFGEVNGLFVNVSEE
                     DKSELLLQVVLSEALKTSEIEGEYFSRAAVMSSLQKQLGILDAPIVSKDKRAQAISTL
                     MLQVRKDYQHSFSLAMLKEWHRTLMKFDKTVKEGEWRSSTEPMQIISGRTGKIEVHFE
                     APPSTELVNLLTGFEKWYHHFPYHEIGQIGRAMLRSALVHLYFETLHPFEDGNGRIGR
                     ALAEKALAETLEKPILVSLSKKIEENKQAYYETLKQAQRKQDVSDWLRYFFTILIEAQ
                     KEVKETVLYVVEKALFFKRYHHQLNERQTKVLQKMTEKGKSGFEGGMTAKKYISITKT
                     SKATATRDLQFLFEIGALIRAGDGRNVKYHLNWTLFS"
     gene            complement(29943..32207)
                     /locus_tag="P5335_00545"
     CDS             complement(29943..32207)
                     /locus_tag="P5335_00545"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRZ73398.1"
                     /translation="MAFDIKMIEKVYATMATRVDKARELVGRPLTLSEKILYSHLWDG
                     NPTTAFTRGKDYVDFAPDRVACQDATAQMALLQFMHAGKKTVAVPTTVHCDHLILAKN
                     GAKSDLALANSQSKEVFDFLSSVSNKYGIGFWKPGSGIIHQIVLENYAFPGGMMIGTD
                     SHTVNAGGLGMLAIGVGGADAVDVMSGMSWELKFPKLIGVKLTGKLSGWTAPKDVILK
                     VADILTVKGGTGAIVEYFGEGATSMSCTGKGTICNMGAEIGATTSTFGYDDSMRRYLA
                     ATGRQDVVNAADKVASYLTADDEVYANPERYFDQLIEINLSELEPYINGPFTPDRGTP
                     VSKMKEEAAANGWPLKVEWGLIGSCTNSSYEDMARAASIVQQAVDNGITPKAEFGINP
                     GSEQIRFTIERDGIIATFEKMGTKVFTNACGPCIGQWDREGADKGEKNTIVHSFNRNF
                     SKRADGNPNTHAFVTSPEMVAALAISGRLDFNPLTDALINDKGEEVMLKTPFGDELPK
                     RGFDVEDAGFQAPAEDGSSVQVAVSPTSDRLQLLAPFEAWDGNNITGAKLLIKAFGKC
                     TTDHISMAGPWLRFRGHLDNISNNMLIGAVNAFNQEANKVKNQLTGTYDAVPAVQRAY
                     KAAGVPSIVVGDHNYGEGSSREHAAMEPRFLGVKAVLVKSFARIHETNLKKQGMLALT
                     FANEADYDKIQENDTINFLDLTEFAPGKPLSLEFVHADGTKDIILANHTYNESQIGWF
                     VAGSALNLIAAGKA"
     gene            complement(32392..33513)
                     /locus_tag="P5335_00550"
     CDS             complement(32392..33513)
                     /locus_tag="P5335_00550"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRZ73399.1"
                     /translation="MSLLHLHSEKRIFGLDLMRAVAILMVLSSHLLWIYPPDNGIISQ
                     VFQLFGYLGVEIFFVLSGFLIGRIVYRLYLQDDFTISSVLYFLKRRWFRTLPNYYLVL
                     LITILISFIVGYSIQEGWKYFFFLQNFASPMLPFFTESWSLSVEEFAYLLFPIALFLK
                     TRLVKPINKSRFFLYVVIGFIFFFFFTKVWYAFTTPPSDINQWNLGLKAVVIYRLDAI
                     FLGVLFSWISLNFTAFWKKGKFVMAVLGLFLFGFLFAGVGYFQLTFASFPLFWAIFYL
                     PLTSLTFALFLPFLAEWKTVHFAIIRKPIVFISVISYSVYLLHYGVVLQLMKHYFNTE
                     GLSLHQLHFYTFIYLIITFFLSTLLYKFYEKPMTDLRDK"
     gene            33547..34710
                     /locus_tag="P5335_00555"
     CDS             33547..34710
                     /locus_tag="P5335_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73400.1"
                     /translation="MAKPKILKITLLLIVLSLAGGVIWYAQLSNRHKAIVKTTFLHKT
                     GLVDSDWKIEKANKEYKMLSPTFIVDNIYKSMEGPKATNTVMLSQDATLLWITGFKVV
                     AQDADTEKRISNDFICHMNVDINDAKYYSHFGLENRIGKQYPRLTSLSHGMESFEFPK
                     GFGIPMKGNDLLFVTTQTLNHNLKEVYKKVKHKVTINYQDKPSLFPLMSKTAFIMLPY
                     DQYDPYKSPTDPGTDFCIPVETKNHSYEDGKGNKLSGHWVIPKGKNTYRSDINHQLEI
                     KDSLRLHAAAIHVHPFATRIMLYNKTTQKPVLVSKITNHKNSIGLTNIENFSSTEGKW
                     LYANQEYEIVLEVNNTSLENQDMMGSMFLFFYDSEMDEILKKSSSQSAKPASQ"
     gene            complement(34691..35644)
                     /locus_tag="P5335_00560"
     CDS             complement(34691..35644)
                     /locus_tag="P5335_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRZ73401.1"
                     /translation="MSDVTAIQTLVQKRLALKKEISKIIVGQDLVVDQVLLSIFSGGH
                     ALLVGVPGLAKTLMVNTIAQALGLDFKRIQFTPDLMPSDILGSEILDENRTFKFIKGP
                     IFSNIILADEINRTPPKTQAALLEAMQERAVTIAGQNYKLSLPYFVLATQNPIEQEGT
                     YPLPEAQLDRFMFSIKLDYPSFAEEVQVVKSTTSDDKPVINSLFTAQEIIDFQHLIRR
                     IPVADNVIEYAVTLVTKTRPDSNLATDFIKNYIDWGAGPRASQNLILAAKANAAFNGK
                     FSPDIEDVKAVAEGILRHRVVKNYKADAEGLSVEAIIAKLV"
     gene            complement(35688..37103)
                     /locus_tag="P5335_00565"
     CDS             complement(35688..37103)
                     /locus_tag="P5335_00565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRZ73402.1"
                     /translation="MKFINNKFLLLFAFAFFTVFATAQEVIQEEAAVKKDTVKKVSQR
                     KKVDGVIATIGDFIILDSDIDMEYIELTSQGISVNDITRCQLLGKLMEDRLYAHQALQ
                     DSTIVVNDGEIQNMMDERLTYMLEQIGSMDKMLKFYNKKNEEEFRSFFFDVLKQGKLS
                     SEMRNKVVADVEITPEEVRTFFKKIPTEELPVFGAELELSQIVIEPKVSDVERQKVID
                     KLIEIRKDVLENGSSFRTRAVLYSQDPGSRASGGFYKMNRKTPFVKEFKDIAFSLNEG
                     EISMPFQTDFGYHIILVEKIKGQEVELRHILLTPKITDDDLREAKEKVTLIRSRIVSG
                     ELTFAEAARSLSDEKETKTNGGILLNPKTLDTRFELTKMDPSLYSQVSSLKPNEVSQP
                     VLDEDQKGMKKYKLLMVTNRIDSHVADYATDYVKIKELALKEKQFEAIGKWSNEKILE
                     TYIRINAEYRDCDFTNNWLKK"
     gene            complement(37087..37938)
                     /locus_tag="P5335_00570"
     CDS             complement(37087..37938)
                     /locus_tag="P5335_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73403.1"
                     /translation="MLKPLGIVLVFFVFVSCSYFKPEAKPEAIARVGESYLYKSDIAD
                     LLPEGTTKEDSLLIVQNFINRWATQKLLINAAELNITKEEQIAFDKLIQQYKVDLYTK
                     AYLEAIVKREVDTAVSNADIKAYYQENKENFRTNGALVQLRYIHLSKEHPKFDLIKSK
                     FFDTKKSDAKFWETYQLQFKGSALNDSVWVEMNQVYQKLPFITPDNSENYIAPGKTIQ
                     YPDSTDVYLVKISNVIDKNEVSPYEYVKSTLKELIINRRKLELIKKFEKEITDDAIKN
                     KNYEIYK"
     gene            complement(37938..39905)
                     /locus_tag="P5335_00575"
     CDS             complement(37938..39905)
                     /locus_tag="P5335_00575"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRZ73404.1"
                     /translation="MTVKQFCLGFAFFVTALLSAQSSNQVLFTINDKPYYTDEFIRVY
                     NKNLDLVKDESQKDLDHYLELFVGYKLKVTKANALGLQNNPQYQNELKSYRTQLSKNY
                     LTDSKVTKELIDEGYARSLKEVRAAHILLTVDENAKPEDTLAAYKSIIELRNRVLKGE
                     DFGQLAFQYSQDPSAKENRGDLGYFSAFRMVYPFETAAFTTPKGAVSQPVRTRFGYHI
                     IEVKDIRDNRGDVTVAHIMLMNPTDDSPTELEKNKTTIFEIYNKIQQGESFEALAKQF
                     SQDKSSASKGGVLNRFGSGQLSSEEFETVAFSLQNPTDISKPFQTQFGWHIMKLIEKH
                     PVKTYEEAKPDIETKIGRDERSRKITESLNEKLRAKYPVKRSDAMFTKVKKTVNDSFY
                     DSTWKLPESMKDFTAPLFTFANQNVSGEQFLNYLYSQQKNPAQVRPVAKLVDFHYSNF
                     VDAELNTYYNDNLENEFPEFAAVMDEYRDGLLLFDLMEKEIWEKSKTDTLGLQAFHTE
                     NANKYLWKKRYDVLIISSTKADIAKKAHKLLKQGKSVEEIKKELNTKDVVNVMTTAGI
                     FEEDNAILPKTVSYQKGLQPVVHEEDYFYVVQVNAVQEAGPKTLAECKGRVINDYQQY
                     LEENWVTNLKQEFNVTVNQSVFDSVKKQIKN"
     gene            40093..41682
                     /locus_tag="P5335_00580"
     CDS             40093..41682
                     /locus_tag="P5335_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRZ73405.1"
                     /translation="MSFIEEINRRRTFGIISHPDAGKTTLTEKLLLFGGAIQEAGAVK
                     NNKIKKAATSDFMEIERQRGISVATSVLAFNYKDKKINILDTPGHKDFAEDTFRTLTA
                     VDSVIVVVDVAKGVEEQTEKLVEVCRMRNIPMIVFINKLDREGKDAFDLMDEVEQKLK
                     LKVTPLSFPIGMGYDFQGIYNLWEKNINLFSGDSRKNIEDTIAFSDLNNPELDKIIGQ
                     KAADKLREELELIGEVYPTFDRKEYLEGHLQPVFFGSALNNFGVRELLDCFIDIAPSP
                     KAKHSDTRLVEPTETKMSGFVFKIHANMDPKHRDRLAFVKIVSGTFERNKPYSHVRLN
                     KSLKFSSPNAFFAEKKEIVDVSYPGDIVGLHDTGNFKIGDTLTEGEIMNFKGVPSFSP
                     EHFRYINNADPMKAKQLEKGVDQLMDEGVAQLFTLEMNNRKIIGTVGALQYEVIQYRL
                     EHEYGAKCTYENFPAHKACWVKPEDPKNEEFAEFKRIKQKFLAHDKYGQLVFLADSEF
                     TIQMTQSKFPSVKLFFTSEFE"
     gene            41804..42016
                     /locus_tag="P5335_00585"
     CDS             41804..42016
                     /locus_tag="P5335_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73406.1"
                     /translation="MKNLYKLYVLAFILLSDLMLFAQPGDDDGTNGGLEGDDIPAAPI
                     NGKLIWLALVGIVFVFYYFNTKRQKA"
     gene            42072..42815
                     /locus_tag="P5335_00590"
     CDS             42072..42815
                     /locus_tag="P5335_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HRZ73407.1"
                     /translation="MIFFSKKKPKLKELIPDNFVDIHSHLIPGIDDGSKSLENTSEMM
                     NSLISLGFEQFITTPHIIQNVWNNNREIIQQGEIAVIESLSSNNQEIPFKAAAEYMLD
                     TSFSELLQKEKLLTLKENYVLVEMSYLNPPMQLVDLIYETQLAGYIPVLAHPERYLFY
                     HNNTSEYEKLKKMGCLFQMNLLSSVGYYGEGVAKTADNLLKKGMIDFVGSDVHHQKHI
                     DAFAGNVILKEMTPLKEAIQNNQLFRFLL"
     gene            complement(42795..>43181)
                     /locus_tag="P5335_00595"
     CDS             complement(42795..>43181)
                     /locus_tag="P5335_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006261956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HRZ73408.1"
                     /translation="PAELLNSTKISDLFDELKKQYDYIVVDTAPVSLVTDTLLVSKFA
                     DVFVYVVRANYLKKHSLIIPKSLHKENKLPNMAMILNGTDPSKTYGYGYGYGYGYGYG
                     YGYGYGADSEPEQLPWHKKILQKKSK"
BASE COUNT        14439 a         8021 c         7236 g        13385 t
ORIGIN      
        1 taataatgat ggaattattg acggtagtac cgatgttgac aaagacggta ttcgtgatgc
       61 gtttgataca aattcagcta aaataggttc tcctagagat ttgattagaa aattattgtt
      121 agatttcgac ggacgaaatg attatgccga agcaaccggt gttttaggcg gattaacaaa
      181 tgctacatta atggcatggg taaatttagc cccgggtttt agctcaaccg gagtagtagt
      241 gggacaaaat aattttcaat tgagaataac cgatacccga gtaattactg cttcttgcaa
      301 cggaacatct gttgactatt cagccattga tcctatcagt tcttcgcaat ggttccatat
      361 tgcgacaacg tatgatggag ctcatttaaa acttttcatt aatggaaaag agatgaatag
      421 cattaacaaa acaggaacta taaatgcgga tgcaacaccc cttaccattg gtagacttcc
      481 gggaaacaat tcgctttatt ttaagggaaa aattgatgaa gtacgtgtgt ttaatgtagc
      541 cttaacagat caacaattgc aacgaatggt ttaccaggaa attcaaaata acagtggtca
      601 ggtgagagga actgttgtcc ctagagatat tgaatcttta ccttttacta atctgttacg
      661 ctattacaga atggatacat acaaagatga tattattgat gacttatcta ccccaactgt
      721 tgatacagga acagggatga aaatgtataa tcataaaaat atttatgctc aacaagctcc
      781 gatgccattt gttacggaaa gaacaggcga ttttgctacc gcagtgaaca gtccaacaaa
      841 cgaagtaaga ggaatggata tcgtagattt taattatgct attgttcaag tgaaacacaa
      901 cattacagaa acatctaatc aaactaattt aggaatgttt gttgatgctg gagtaacaat
      961 tacgatgaat aacgatacaa aaattcaaaa caattggtat gtgaatttaa acggaaaaat
     1021 tgatttagta gatcgttcac aattcgttca aaccgcaact agtgtattag acccaaccag
     1081 ctctggttcg cttgaaagag accaacaagg acaaagcaat aaatttaatt ataactattg
     1141 gtcttcaccg gtgagtgcta tcaacagttc aacaattaac cacggttata ccgttgcagg
     1201 cgttatgaaa gacggaacaa acccatcaag tccaatgaat ttagcttggg taagtggcat
     1261 aaacaatccg ttaaccagta acccaattac gttgagcagc tattggatat tcaaatttca
     1321 aaacctaacg cctgtttatg caaattgggc aagtgtaggt caaaatggtc ttttgctggc
     1381 cggtcaaggc tataccctta aaggtagtgg agcagcaagt gcctctcaaa actttacctt
     1441 tattggaaaa ccaaataatg ggcaaattat cagcactgtc tcagccgaca acctaaactt
     1501 atccggtaac ccatatcctt ctgctttaga tgcggataaa tttatacaag ataacttagc
     1561 gaccatcaac ggaacattat acttctggga acatttcaac acaaataata cccatacttt
     1621 agcgggttac cagggtggat atgctgcccg taccttagta ggcggaacag ctcctgtatc
     1681 tccatctggc atcagtggat tgggttcaag tacaagaact cccggcagat tcataccggt
     1741 aggacaagga ttctttgtga caggaaatgc aactggtggt gatattgttt ttgataacag
     1801 tcaacgtctt tttatcaaag aaagtaatgt tgattctaac atcctattcc gaactaatca
     1861 aaatgcttct tcaccaaatt taggaacagg aaataacgag gaagaccctt atacagaaaa
     1921 tgctttcatg aaatttcgta tcggattcaa ttcttacaac aatttccaca gacaactatt
     1981 aattggtttt atggatgatt tagcaacaga atctgttgat ccgggttatg atgcaattca
     2041 tattgataac caacaaaatg acatgtattt tttaaataac ggaacaaaat taatcattca
     2101 aggtgcttcg cattttgaaa cgaataaagt ttatccgcta ggaattaaaa cctatgcagc
     2161 cggtgatatt caatttactt tagatggttt agaaaatgta gagccaacac aacctattta
     2221 cattcatgat atagtaaccg gtttatacca tgatatcaga aatgaacatt ttacaatcaa
     2281 tttgccgcaa ggtgaaatcc acaatcgttt tgaactgact tttcaaaatc atgctttatc
     2341 agcatcggat tttgaacttg aaaatcaaat cgctgtggct tatactaacg ccaacaattt
     2401 acttactata aaaaatacca tgaacgacaa tactgtggaa acagttagtc tttttacaat
     2461 cttaggtcag acccttgctt cttggaatgt tgaaaaccaa acccaaaaca acatttctat
     2521 cccaatgagc aacgtaagta ctggcaccta tattgttcga gtgcaaacca ctcaaggaac
     2581 aattagcaaa aaaatcatta ttaaataggg ttttgataat tgatttttga caccgaaaag
     2641 cccctcgacg tgaggggctt ttcattttta ataaatagaa gcttataacc aaactgaggg
     2701 aacggaaaat atttttttta agttaaaaga ccataagcct gctccgccat ttcaaactcc
     2761 tcattcgttg gaatgactaa aattggcact ttagatcctt ctaatgaaat agaacgaata
     2821 tttttggaac gcaacttgtt tttttcttca tccaattgaa tgccaaagta atccatgtgc
     2881 tgacaaatta atgaacgcat cacatcactg ttttcaccta ttcctgctgt aaataccaat
     2941 acatccaaac cattcaatgc cgcaatataa gaccctataa atttttgtac acgataagcc
     3001 atcatctgaa gggcaagcaa acactcttta ttcccttttt gggcctcgtt ttgtatatct
     3061 cgtaaatcag aaaaaccggt caagcctttt aaaccactct ctttttgcaa taaatcttgt
     3121 acttgactcg aagaatagtt caaggtatcc attaagtaaa aaataatcgc ttgatcaaca
     3181 tcaccactgc gggttcccat tatcaaaccg ttggtaggtc cataacccat agaatgttca
     3241 atgctttctc catctttcac agcggtaata ctgcaaccgt ttcctaaatg tacagtgata
     3301 attttagaat gtttgagcct caaaaattca atggcttttt tagaaacgta tttgtggctc
     3361 gtcccgtgaa aaccatattg tcgtatctta tgtttatcat aaaattcatt cggaatagaa
     3421 tagcgatagg ctttttcagg tattgtctga tgaaaagccg tatcaaatac agcgacttgt
     3481 gtagcctttg ggaaaacgac ttcggctact tcaatacaag ccaaattagc tggattatgc
     3541 aagggagcca acgcacataa ttcgcgaatc ttatccttaa cttctttggt tataatcgtg
     3601 gtttttgaaa actgttcacc tccgtgaacc acacgatgaa cgatggtatc aattgttctt
     3661 cctgaacttt tactcaaaat tttttgagct attgcttcca aagcttcttt gtgattggag
     3721 aattcaacag cttctgaaaa ttcttccgat tgaatcgtat agtgaaattt agtatgttcc
     3781 aaccctattc gctccaccaa acccgaagaa ataacggttc ccaatggcat ttcaataagc
     3841 tgaaatttta atgacgaact tccggcatta atgactaata ggttcatagg tagtttttaa
     3901 atatcttgtg cctgaatggc ggttataatt atcgtgttgt aaacatcttc aacggtgcaa
     3961 cctctactca aatcgtttac gggcttattt aaaccttgta acattggtcc gatagcaacc
     4021 gcaccggttt ctctctgaac ggctttatag gtattgttac ccgtgtttaa atcggggaag
     4081 attaatacat ttgcttgacc ggcaaccaaa gaatcgggca ttttactttt accaacagcc
     4141 atatcaacgg ccgcatcgta ttgaattggt ccttcaattt gtaaatcggg acgcaaagtt
     4201 ttaaccaatt cagttgcttt tctaacccgt tccacatctt cacccattcc tgaacttccc
     4261 gaggagtaag acaacatggc aattttaggc tcaattccaa atgcttgact gctttctgca
     4321 gaggagatgg cgatttgagc taattcttct gctgttggat tgggattgat ggcacagtcg
     4381 ccaaaaaccg aaatacgatc ttccaaacac atgaaaaaca ccgaagaaac tactgaaatt
     4441 ccttttttag ttttaataat ttgtaaagct ggacgaatcg tatgttgtgt ggtatgaata
     4501 gccccggaaa ccattccatc tgccaatcct ttgaaaacca tcatagtgcc aaaataagag
     4561 acatctgaca tcaaatcttt ggcaatatcg tgattcacat ttttatgttt tctcaattca
     4621 tagaatgtct caacaaaatc agcataatag gcagagttta ccgggtcaat aatactaact
     4681 ttatccaagg gtagaagaat gccggactta atcaaactct tttcgatagt ctctttttta
     4741 cccaacaagg tgatatccac cacatcggca gcaattaatc gggcagttgc ctctaaaatt
     4801 cgtaaatcct ccgattccgg cagaacgata tgtttacgta cttttctcgc cttttttaat
     4861 aaattgtatt gaaacatttt tggcgtaatt ccttttgagg taaaattagt tacattctca
     4921 atcagtaaat cggaatcaac atatttataa aatgtttcaa gggataattg aattttcgtg
     4981 gtatcaccgg gtcttatttt acaatttata cgacctattt tattagccgc tttaaaagtg
     5041 ttttcttcca cactaatgat aggaataata tcatctaacc cttcaatcaa tcttagaata
     5101 gactcttctg gaataagtcc acccgttaaa ataattccaa ccattctggg ataactggcc
     5161 gatttgtggg cttgcaaaac ccctatgata atgtctgccc ggtccccggg agtaatcacc
     5221 aaattatttt gcccaatgtg agtgaggtaa ttgtgtaatt gcatagcacc cacactaaaa
     5281 ctgcctacta ttccctctaa caaatgctcg ccaaataaaa cctttccatt caacgcttct
     5341 actacttccc gaacactggg gttgctcaat aattcattga ccggtatggc taaaatttga
     5401 acctcatcag cacaaaattt ttgaattcct tcaataaatt ttgaacgatt tttctcttga
     5461 attttattga taacaatagc cattacgcca acttcctttc ctaaaaagga cttgtaagct
     5521 agttctaaat tttgaatggc atcctctata gaattgtctc ccgaggcatt aatcagcacc
     5581 ggtaatccta aatttttagc aatttggagg ttaatttcaa attcaaaaac ggaaacttca
     5641 tcttcaaaat cgcttccttc cactaaaaca aaatcaaatt cttcttccaa ttttttgtaa
     5701 tcattcataa tcgtatctat cacctcccct ttctttccca aattaaccaa ctgaatcatc
     5761 tgttgtttgg tataagaata cgcattctca tagtcaactt ttagattaaa agtttgaaga
     5821 attaattgca aatggttatc aactttagat ttcttccctt cgttaatgat gggtcggtaa
     5881 taagctactt ttgccatacg gcttagcaat gcatccatta atcctaaact caagagcgat
     5941 ttaccacttc tgggttcaat agtggcaata tataatcctt tgttcatagg ttgttatttt
     6001 gattgtgtgg tattgattga ttatactccg gaatatacat tttactttca ataaatgttc
     6061 taatgttttc aggttttgaa accccggcta aaccggaatc aaatatatgt tcggcaaccc
     6121 tgacagctac gtttatagaa acttttaaaa tattattcac ttgcggataa attaaaccgt
     6181 tttcaaaatc tgcagcagtc acttgttctg ctactgcttc agaagcagtt aacaacatat
     6241 catccgttac tcttctggct tcagtggcaa aaatagccat tcccaaagca ggaaaaataa
     6301 agacattgtt tccttgtcct ggtgtataga ttttcccttc atagtttaca ggggcaaacg
     6361 gacttccact agcgaaaata gctttccctt tactccaagt ataggcttgc tcggctgtac
     6421 attctgaatg cgaagtggga ttggaataag gaaaaataat cggacgttca ttcaccaaac
     6481 tcatattttc aataacctgc tgattaaatg ctccaccaac tgtacttacg ccaacaatag
     6541 cagtgggttt aattttcaat accgcttcag caaaattggt acaagcatca aaatcatggg
     6601 cgaaagccaa ttgatgggga gccaaatctg ttctgctttt caccaataaa ccattcacgt
     6661 caaacatcca aatttgttga taagcttcct cctcagacaa tccatcacgt tgaaattttt
     6721 tggccaacat atcggcaatc ccaaaggcgg cagctccggc acctaaaaac agaaaacgtt
     6781 ggtctttaaa cgatttgttc attaatcgac taatggatat aaaaccggcc gttgcaatgg
     6841 ctgcagtgcc ttgaatatca tcgttgaagg tacacgtttt gtcgcgatag gtttccaata
     6901 tacgaatagc atcaaccccg gtaaaatctt cccattggat acatgctttt ggaaaaactt
     6961 cattcatggc ttcaacaaaa gcagctacaa atgcatcaaa ttcaggacct ctaatccnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc ttttcaaaat tgtccaattg
     7141 ctcttccaca cgtaaaattt gagtagcaag ggtttctata gtatccggta aaattccttt
     7201 caatttgaat ttttcacgtt cttccattgt aaaagcagtt cctttattaa aacgtggatt
     7261 tcgcaacagt gaataacctg taatagcttc catgaggttt tattttttac ttttttgaaa
     7321 tagatttact taatacaaaa tcatttttaa aaacaattta atattatcgt ataaagttaa
     7381 gaattccata ttaaccgaac aactattact aacctttata atggagtttt ttctttttaa
     7441 tacactcttt aactactttc ttaaaaaaat aactacagta catttaataa taaaatatat
     7501 ttaacagaac ttatatgaaa aaaaaagccc caaagaattg gagctcagat tagtatagaa
     7561 ttgtattaat taagacaatg ctttctttaa agattcaaaa ttcaacttta cttgctctcc
     7621 ggtttgcagg tttttgagtc caaattccat acgctccaat tctgattgac cggcaataac
     7681 ggcaaaagga atgccgcgtt tatctgcata ttgaaattgc ttggcaattt tagcggcatc
     7741 cggatataat tcgactttaa tgccggcacg acgtaatttg gaaatagctt ccatcgcata
     7801 caaactttcg tgatttccgt aattgataaa caacgctttt gtagttgccg aaactgtatc
     7861 aggaaataaa cccaattctt ctaaaaccaa ataaattcgg tctaacccaa aggatatccc
     7921 aaccccactc atgtttttca atccgaaaat tccggtcaaa tcatcgtatc gaccgcctcc
     7981 gccaatggaa cccatggcga cttgaggtgc aacaacttca aaaatagctc cggtataata
     8041 atttaatcct ctcgccaaag taacatctaa atcaataagg gctgtttgta aacctaattt
     8101 ttcaacggtt tcgcaaatga atcgcaattc ttccacacct ttcaatcctt caactgatgc
     8161 agctagtagt tgatttagtt tttctatttt ttgaataacg tttcctgtaa attgaaacaa
     8221 cggttgcact ttttctattg ctgaggcaga aattcctttt tcaagcattt ctttttttac
     8281 tccctcctcg cctattttgt ctaatttgtc taacgcgact gtaaaatcaa tcaatttgtc
     8341 tttcgcccca atgacttcgg caatacctga aagaattttt cgattattaa ttttaatagt
     8401 tgttccacct aatcctaaat cagtaaaaac cgcatcatac aattgcacca actctacttc
     8461 ctgccacaac gaagtagaac ctactacatc ggcatcgcat tggtaaaact ctctgaatct
     8521 acctttttgc ggacggtcag cacgccaaac gggttgaatt tggtagcgtt taaacggaaa
     8581 ctcaatttca ttttggtgtt gcacgacata acgggcaaaa ggaacagtta ggtcgtaacg
     8641 aagggctttt tcggaaattt tgccggtaag ttttttataa tcgataacat tcaattcatc
     8701 ggaaccaact ttatccaaat aatctccaga attcaatatc ttaaaaatca atcggtctcc
     8761 ttcttcccca tattttccca tcaacgtttc tgaattttca aacgaaggtg tttcaatcgg
     8821 ctgaaaacca aaaacttcaa aatgatgctt cattatcgaa ctgatgtagt ttcgtttcgc
     8881 cacctctgtc ggtgaaaaat ctctggttcc tttgggtata cttggttttt gagccattgt
     8941 tatagttata ttttttatcg tctttgctat tgtcctttac tttgcaaata tcttactttt
     9001 taaagaaaat aaagaaacct tgtaacaatt attgtaattt cgtgtcaaat taagcatatg
     9061 gctcgattag ttaaagaaaa tatcaaaatt gcactgggtt ctattaaaac acagttgctt
     9121 cgtactatcc tgacaattgt aattatcgca ataggaatta ctgcgttggt aagtatttta
     9181 acggtagtat cggctcttga aaataccttg tcaaccaatt ttgcgtctat gggagccaat
     9241 acgtttacca ttcgccaata tgaattggaa gtgcgtgtgg gaaatggtcg cagacaagaa
     9301 cgcgaaatca tcaatccgat tatctcctat ccggaagcaa aagcatttca agaaaaatac
     9361 aattatcctt tcacccatgc ctctttatca ttcaccgcta cttcagctgc cgaagtaaaa
     9421 tttgaaaata aaaaaaccga ccctgaaatt actgttgtag gtgttgatga ttacttttta
     9481 actaattccg gactggaatt agccgatggt agaaacttta ccggtttcga tattgaaaat
     9541 aataattttg tttgtgtagt aggctccgat tttgaaaatg gccttttaaa agatgtcaac
     9601 cctatcgata aaatcatttc tattcgtggg gccaaattta aagtcattgg aatacttaaa
     9661 gaaaaaggtt caacctttgg taatagtcag gatttaaggg taatgattcc aatccaggta
     9721 gctcgttcac tttttacaca acccaatatt aattataacc ttagtgtttt agtagacaaa
     9781 aaagagttat tagatgctgc catcgacaac gcaatcatta cgatgcgaaa agttcgaaaa
     9841 caaaatccgg ttcaggacaa taattttgga attgttcgaa gtgacgaatt aatcaatcaa
     9901 attctatcgc taacttctgt tttgggaatt tcggcgtgga taattggggt aatcaccata
     9961 tttggttctt ctatcgcttt aatgaatatt atgctggttt ccgtgacaga acgaaccaga
    10021 gaaattggtg taagaaaagc gttgggagct aaaaaaagta cgattgcttt tcagtttttt
    10081 accgaaacaa ttgttattgg ccaattaggt gggattcttg gtattatttt aggtatatca
    10141 ataggctctg cttttgcagc cttactcgaa tttgatttta ttataccttg gatggcaatc
    10201 attgccgcgg taattacgag ctttttagtt gctgttgttt ccggtttata tccggctata
    10261 aaagcggcaa aactagaccc gattgaagca ttacgttacg aataataatt tttccttttt
    10321 ctaaaaactt tttaccgctc tgagcattcg gtcatttccg taaatatcct gtcgaagttc
    10381 tatttctgta aaaccataag aagctactaa tgcaatcatt tcagtcccta aatactgatt
    10441 gatttcaaaa aacaattgtc cgttaggctg taaatattct tgagccaatt gagcgatttt
    10501 tcgataaaac aacaaagcat cattatctgc aacaaataaa gccaaatggg gttcaaattg
    10561 taaaacgttg gtatttattt cctttttttc aagctcccga acataaggcg gattcgaaac
    10621 aattacgtca aattgctgct ccaaccttgt ggttgctaaa atatcatttt gtagaaaagt
    10681 tacatccact tgattcattg aagcgttttt agtggcaact tctaaagctt tctccgaaac
    10741 atctgaggca aaaacggttg cttttggtaa attctttttc aatgtaatcg gaatacaacc
    10801 ggttccggtt ccaatatcca atagtgcaat ttctgaacta gttggattgg tttcaagaat
    10861 ccattgcacc aattcttctg tttccggtct cggaatcaag gtatactcat ttaccagaaa
    10921 agttaaccca taaaaagagg tttctcctaa aatgtattga atgggttttt gcgttttcag
    10981 ttggattaaa acagaattcc aatactgttc ttcttcaaca gtgatttcaa aatttggatt
    11041 caaagccaag tcaacacgct tcaattgata cctcttttct aaggttaaat agaaaaactg
    11101 atcaatctcc attgcatcaa aaaagggcga cagttcgttt tgaaaaaggg ctttgtattt
    11161 ttttacttcc attgaattta attacaagtc ttttaacatc caaaccggac aagaggtatg
    11221 tccggtgcaa cccataggag catctatata ctgaaaacca aattttttat acaatttctg
    11281 agcattttcc atgtaaggca tggtttccaa ataacatttc tcatacttaa aagagactgc
    11341 ggcttgcaaa cacttctcca tcatttgaga tcctaatcct agcccacggg cttccggcaa
    11401 aaaatacatt ttttgtaact cacaaatagt ctctgcttca tttgctaact gactcacacc
    11461 ggctccgcct ataattttac cattattttc tacaacaaaa taagaacttc cggggatatt
    11521 gtacgtttca aacatggaat ccaaagaaac atctgcataa gcagttccaa cttttggaac
    11581 attgtgttca attaaaacgg aacggataac agctgctata gcttggttat cagtgttaag
    11641 tataggtcga atgattatca tacaaatgaa tagctatttt aaaactgtaa aagtaaaact
    11701 atttcaactt tattagtcaa aaagcgtaat tttgtcttac catgaatcat gaaatttaca
    11761 tccaacgttg cctcgaatta gctcaaaatg ggttgggttc tacctatcct aatccaatgg
    11821 taggtagcgt aatcgtgcat cggggaaaga taattgggga aggttggcat caaaaagcag
    11881 gagaaccaca tgctgaagtc aacgctatta attccgtaaa aaataaatca ctgctaacta
    11941 agtcgactat ctatgtgagc ttggaaccat gcagtcattt tggaaaaacc cctccttgca
    12001 gtgacttaat tatccaacat aaaataccaa cggtaatcat cggaactgtt gaccctaatg
    12061 ataaagtagc cggaaaaggc atagaaaaac ttcaaaaagc aggaattaaa gttaaggttg
    12121 gcgttttaga aaaagaatgc cgggaattga ataaacgttt ttttactttt catactaaaa
    12181 aacgaccata tatcattttg aaatgggctg aaagtgccga tggttttctt tcaccattga
    12241 ataaaaaaga gcaaaaaccg gtttggatta ccaatgtgta tgcacgacaa ttggttcaca
    12301 aatggagaac ggaagaacaa gcaatactag ttggaaccaa aactgtttta gacgataacc
    12361 cccaattaaa tgcccgggat tggtttggaa aaaatccaat tcgaattatt ttagatcgct
    12421 ctggaaaaat ttctgaagat tatgccgtaa aaaaaggaag tcaaaaatca atcatactga
    12481 cggaacagga aattttaaca aaagatgaaa attgtattta tgaaaatctt atctttggag
    12541 aacaacttcc tgaaaaaatc agtaatttgc tttatcaata tcaaattcaa tctgtcattg
    12601 tagaaggagg acgtcaaacc ttacaatctt ttatcgacga tgatttatgg gatgaagctc
    12661 gcatttttaa aggaaatata atattacacc aaggaactaa agccccaatc ctaaaaggtg
    12721 aaattgtagt aaaacaaaaa agcataaagg acgaacttat aatcattaaa agccatgatt
    12781 aacactatca tttttgattt cggaaacata ttcattgatt tggataaagc tgcggtagac
    12841 aaagcctttc gtgatttagg tttaaccgaa tggcacgctg atttagatga attaaataaa
    12901 cgttttgaag tgggcaaagc aacagaattg gaatttattc aaggctttca aaaacattta
    12961 ccaaacgctt ctattgaaga cataagaaaa gcttggaatg cagttttggg cgatttccct
    13021 ttgaaaaggt tagaatttct acaaagctta aaaggcaaat accgtatgtt tttattgacc
    13081 aatacggata tgattcacat tgaccgtttc gaacataaag aaggtatgtc gattacacgg
    13141 gatttctaca actgtttcga aaaagtatat tattcctatg aaatgggaat tcgtaaaccg
    13201 aatgtagaag tctttaaatt cctattgcat aaacatgaat tatcacccaa acgaactttg
    13261 tttattgacg atagaaaaga aaatacggat gcagcagctt ccttaggtat tcaagtatgg
    13321 aatttacaag ttggagaaga agaggtaact gatttattcg ccaaaaaaat aattagcttg
    13381 tgatttatat tgttttaagt gttcttttca acgccgttct ttttgtaatt ctaaaactgt
    13441 tttctcgtta taatatcaat acactccaag ccttagtagt caattatttt actgcttttg
    13501 tgatgggatt aatactttct gaccaatcgt ttaacttgtc cgaaatagtt cacaaaccat
    13561 ggtatccggg cagtttcatg ttgggatttt tgtttatttc tgtattttat gtcacggcgg
    13621 ttacttcgca acgaaacgga ctttctgtag cctcggtggc ctcaaaaatg tctgtcatta
    13681 ttccaatttc ttttggcgtt tggctttttg gcgaagaatt gggaatagcc aaaattttag
    13741 ggattatttt agctttaatt gccgtttatt tcaccgctaa aaaggaagaa ggagctgtag
    13801 cagattccaa aaatttgatt ttccctacat tggttttttt tggtgccgga gcaattgata
    13861 ccagtctgaa agtattacag aatgactatc ttccgactga ggaaatttca ttattttctt
    13921 ctcacacctt tttaatggct tttacaattg gtgttttttt aattggaatc gctatcatta
    13981 aaaataaaat gaaaataacc ggtaaaagta ttttgggagg tattgtatta ggtataccaa
    14041 attatttttc actctactac ttaattaaaa tgttagacag tgaagtattt gaaagttcta
    14101 ccattttcac cattcacaat gtggcaatcg taatggtttc aacattggtt ggtatcgtat
    14161 ttttcaaaga aaaaatatcg attcgaaacg gaattggaat agcattggca ctcacagcta
    14221 tctttttagt aaccagttaa aaaataatgg ctgattcaaa agatacatat aaaacaatac
    14281 tcacctcttc agaagaagtc ttgttcaaag aaaaaaacag taaatttttt gggtatgcat
    14341 ttccggttgc caatgaagaa gaagtgaaaa tacagttaga gcatctaaaa aaacaacatt
    14401 tttcagcaag acattggtgc tatgcttatc aattaggtac cgaaaaaatc agttttaggg
    14461 ccaatgatga tggcgaacca aataacagtg ccggaatgcc tatttacgga caaatccagt
    14521 cttttgaggt aaccaatgta ttggtagtgg ttgttcggta tttcggaggt gtaaaattag
    14581 gtgtaggcgg actcatatct gcctacaaaa caaccgctca aatggcattg gaaaatgcag
    14641 aaatcatcga aaaaaccatt gacctttatt atactattgt ttttgattat aaaaacatga
    14701 acaaaattat gcgaatcatc aaagaaaaaa acttggaaat tgtctctcaa aaaatggaat
    14761 taaattgcga aatcaaaata gcaacccgaa aaaaaaatgc cgaaatgatt ttcgacattt
    14821 tttcttctct ttttgaagtt tccataaaaa aggaaaatta atcttctaat aattccatgc
    14881 tgtcaataat cgattttata tttgaaggag gcaacatggg tttgccggtt tttaggtcaa
    14941 caaagaccaa aataaaatcg gctgttgtta ataactcccc tttttcactt tgaattttac
    15001 attcaaattc tatcttcacg gagctctgct ttttgtattt ggttatgact gtaagtaagt
    15061 catcataacg tgctggcttt ttgtaactta tattgatatt aacaatagga agggcaaccc
    15121 cgctttcttc catgctttta taagaaatcc catgattcct aagccattcc accctaccta
    15181 tctcaaaata aggcaaatag ttaccatgat acactacgcc catttggtca gtttccgaat
    15241 agcgaacacg gacttgtaat tgatgttctt tcatactttt ctgtaatttt tttcttagta
    15301 tatttttgaa gccttaagac gtctttacaa caatttttct ataaaatcaa tagcatttta
    15361 ttttttttta agtttttttg ttcacatatt tgttatccca aaagttagta aagaaatggc
    15421 ctaatttctt tgtaagataa cctttagtaa acaatattat ttttagaatt tatgagcaaa
    15481 actgcggaat cggtatggga aaactgtcta tcatttataa aagacaatat tcaggagcaa
    15541 gcttacaaaa cttggttcga acctattcgg tcagtggaac tcacagacaa tgctttatac
    15601 attcaagtac ccagcaaatt tttctatgaa tggctagaag aacactatgt gaaattactc
    15661 aaagtggcct taaccaaaga acttggaaaa aatgcaaagt tactctataa aatcaaaatg
    15721 gaaaacactt atggcaataa acaaccgttt acggaacaat tgcctagtgc tcaccgtagt
    15781 ccggttaaac cacaagatgt tgacgctcca ttaaaaaaca ttagtcctga acttaaaaat
    15841 ccatttgtga ttcccggtat cagaaacctg aaaatagaat cacagttaaa tgcaaattac
    15901 agttttgata attttctgga aggagattca aaccgtttag ctcgttctgc aggaatggct
    15961 gtagccaata aaccgggagg aacttctttt aatccgcttt taatttttgg tggtgttggt
    16021 ctgggaaaaa cccacttggc tcatgctatt ggtgttgaaa tcaaagacaa atacccggac
    16081 aaaaccgtat tgtatatttc agcagaggtt tttacacaac aatacattga ctcggttaag
    16141 aaaaatacac gaaacgattt tatacatttt tatcaattaa ttgatgtatt aattattgat
    16201 gatgtacaat ttctttccgg aaaatccgga actcaagatg tgtttttcca tatttttaat
    16261 ttcctgcacc aaaatggcaa acaggttatt ttaacctcgg ataaagctcc tgtcgatatg
    16321 caggacattg aacaacgttt attatctcgt tttaaatggg gattgtctgc cgaattacac
    16381 caaccggatt atgaaacccg tatttccatt ctgaaaaaca ttttataccg tgacggagta
    16441 gaaattccgg aagaaatcat tgaatatgtt gcccgaaata tcaaatcgaa tgttagggaa
    16501 ttagaaggag ctattatttc attgattgct caatcttctt tcaataaaaa agaagtaaca
    16561 ttagatttag caaaaaatgt cgtagagaag tttgtaaaaa atgtcaaacg cgaaatttct
    16621 atcgattaca ttcaaaaagt agtgtccgat tatttccagt tagatttaga tacattacaa
    16681 tctaaaaccc gtaaacgaca tgtcgtgcaa gcaagacagt tagccatgtt ctttgccaag
    16741 aaatttacca aagcttcctt agcaaacata ggctcgcaaa ttggcgaccg tgaccacgct
    16801 accgttttac atgcttgcaa aacagtggac aatttagtaa caacagataa gcaattcaga
    16861 aaatttgtgg atgatattca taaaaaactt tctttataaa aaatagtttc agtattctta
    16921 gtacttttga atgctgaaat tttttagttt actattttat gcctgtaaaa atattgatgg
    16981 tttgtttggg aaacatttgt cgttcgcctt tggcggaagg tattttagcc tccaaacttc
    17041 caaaagaaaa attttttgtc gactctgccg gaaccggtca ttggcacgtc ggaaaccctc
    17101 ctgacaaacg ctctatctta acagccgaaa aaaatggcat caatatttcc aaccaaagag
    17161 gaaaattgtt taaaccctct ttctttaaag aatatgacca tatttatgta atggacacct
    17221 ctaactatgc tgatgttatt agtatggctc ccgatgagga agcaaagcaa aaagtgatgt
    17281 taatccttga tgagcttttt ccgggtgaaa atgtggatgt tccggatcct tatcatggta
    17341 tacaaagtaa ttttgaccaa gttttcgaaa tgttggaaga agcctgtgat accattgcaa
    17401 ccaaaatgtt ggaaaaatat tccgaagaat gaaacccatc tccttcatag gaaaattata
    17461 tttgataccc actaccttag gtgacatatc acccgatgag gtgttgccac aaacggtaaa
    17521 aagagccatt gatttcatca acgattatgt ggtagaaaac gaaaaaacag ctcggaaatt
    17581 tataaaatca atcaatgctg aaaaagttca ggccaattta agacttcatg tcctgaataa
    17641 atttacagaa gtttccgaat acaacgaaat gcttaaacct tgtttagaag gtcgcaacgt
    17701 tggcttaatg agtgaagcag gttgccctgg cgtagccgac ccgggtgccg tgattgtaaa
    17761 acttgcccat gaaaaaggta ttcaagtggt tccgttagtg ggcccctcct ctattctgtt
    17821 ggctttaatg ggttccggaa tgaatggaca gagttttgct ttcaatggtt atctacccat
    17881 cgataaaagt gaaaaaaaat cggctttaaa aaacttagaa cgcctttcga tggaaaaaaa
    17941 tcaagctcag atttttatcg aaacacccta tcgtaataac aaattattag aagatatttt
    18001 gcaatcgcta caaggttcta ctcgcttatg tgttgcaaca gatattacct taccaaccga
    18061 atacattaaa accctgacca taaacgattg gaaaaaaaca aaggtcgatt tacacaaccg
    18121 acctactatc tttattattc aaaaatcgta acaccgcact aaaagtagaa ttggacttca
    18181 agaatagttt gtaggatgaa aaattatttt gggaagtttt aaattagtct tttctatttt
    18241 acaaatttat atattgataa aaatatttta gcaaaaaaga ttattttgta tttttacaaa
    18301 aatattcgag tatggaagat tatttcaaca aactaaaaaa atacaacttc tgggaaggaa
    18361 atgtaccttc attgggcttt gaacgtaaaa cctattcaga tgctatactt gattataccg
    18421 gcaacaaact aattaaagtc ttaacgggac aacgacgggt aggaaaaagt tatttacttc
    18481 gtcaaatagc tgataaaatc ataaaacaag gcatttcggc cgaaaacata gtttacttga
    18541 acaaagaatt tacagatttt gattttatag ggaattacaa agagttagaa aaactcatag
    18601 aaatatatag aaacaaaata aatccgaaag gaaaaattta tttgtttatt gacgaagtac
    18661 agaatattga gggttgggaa cattttgtaa attcctattc acaaaattat gtggacagtt
    18721 atgaaatttt cattagcggt tcaaactcca aaatgttatc cggtgaattg gcaacactgc
    18781 tttcaggtag atatgtttca tttgaaattt ttccatttgg attcagcgag tatgcacagg
    18841 ttctggaaaa agaaaaaaat aaacaaacct atattgaata tatggaaagt ggaggcttac
    18901 cagaactttt tgaattaccc aatgaacaaa cgaaacgcaa ttatgtttct gctataaaag
    18961 attctgttct tttaagagat atcatccaac gttacaatat caaagaccct agattgttag
    19021 aagatatttt tgtttatctg gtcaacaatg cctcaaattt gatctcaata aacaacattg
    19081 tcaatttttt taaaagtaac aaccggaaaa caacctatga taccgtttct aattatatcg
    19141 aatacataac cgatactttt cttattcata aagcagaacg atatgatgtg aaaggaaaag
    19201 atattatttt aggcaattgt aaatattaca tcaatgattt agcttttaaa aactacctat
    19261 accaaggatt tggttatggt attggttata aactggaaaa tctgatctat cttgaactcc
    19321 gaagatcggg ttacgaggta tttacaggaa ctatcaaaaa tagagaaata gactttgttg
    19381 ccaaaaaagc aaaccggata atctatctac aggttaccta tttgcttgaa aatgaacaaa
    19441 cagcaactag agaatacgct cccttaaaag ccataccaga taattacgaa aaagttgtgg
    19501 tctccttaga cgatgtttca ttaccaccta acgaggggat attgcatgta caaacttgga
    19561 aattggcaga ttttttaaaa taacaagaaa cctgtctttt gttagtttag atttaaaaaa
    19621 aataacaaat tcgtattaaa ctagaactca aaaaagttaa aaaatagaaa aagtcgattt
    19681 acacaatcga cctactatct ttattattca aaaatcgtag taattactta actttagctt
    19741 tcccattggc aacaatatgc gaggtgtcat atccgccaaa ttgtttgatg taacttttca
    19801 aggaagtccc gtaaccatct tttaaagctc gtttaccatt gcttttcaaa aactttttaa
    19861 ctgaaccgga accacctaaa tgggcggccg ctaacaatcc cgattctgtg attattacac
    19921 cattaatggt tttgccttta tacttttcaa tttcattttt caaaacatgt ttattgattg
    19981 ctaaaagagc tttaaatgct ttttcttgta aatgaggtga attcataaaa gcagcactat
    20041 cggtaatacc taaggtacga agcgtcccaa caccaaactg gtatttgccc atataaccta
    20101 atgtattaac taaattatac cgcccttgtg attctttaaa actgagagct tgcttaaatc
    20161 cggtaaaagt ttttccggtt tgtggaatgt ttaaatttac atattgtaat tcctcttggg
    20221 taggaacagt ataatttaat ccttctattg catgatccac ataaaaccaa ttcatttttt
    20281 ctatttcaaa aggtttaaaa cctgaactga ttagggccac tattagggca atactagaat
    20341 aaaaatacca ttttttcgac ataccaattt gtttctcaag tgctgtcacc ggcttgaatt
    20401 tcacggggca aatatacaac tttttattat aaatttgaca atcagcgagt taaaatttcc
    20461 taaaagtaga ataggtaaaa tctagtgcct ccggaggtat tgatttcaaa cgttggagcc
    20521 ggaatcttaa ccgaattgaa aatagaaaaa agggtgctta aaaaaggtga tttcgtttta
    20581 attttagaca aatttacatc taaactcaga taaaattggc gaaacctttc gtcattttca
    20641 gcaggtattg ccgtaatcat tccctctgct ccatatccta aagctacatt taaccaggaa
    20701 ggtattggtg tttgttttaa aaaagaatgc aaattgacag ataaccaata cgtttgtccg
    20761 ttataatctt tcaaaatttg ctcacttaat gaactgccta acacgtccgg tctttgatca
    20821 gcataagctg ttgtatgaaa cgaaaacttc ggaatgattc gttgctcatt ccacaacaag
    20881 gattgcgaaa cataaagtgc cgttccggta gcattggctg ccacatcgcc ccaagaaaat
    20941 ccccattcag acgaataacc gtcaaatacc tcaaccgcag ttaaaaacac aaatccatag
    21001 cctgcaccgt atagtaattg attttttttt gaaacaccgg accatgctaa catttctgca
    21061 ctgaatcgcc caagttgata agccgaataa acatgaccca atttatccat ctgcaaccaa
    21121 ttggcattgt cattcgcata atgaaaatca gattttggat aatccgcata ccaaagctca
    21181 tttaaaccta ccaatgccgt agcagttaat actgattctg taataataac cgtatttctc
    21241 cgagagatat gtaaggtatc ggaaggtgtg aaaaaagtat caaaccctga ttgcgaaaaa
    21301 acaatgttcg aaaagaacac agcaaaaagg aatacaacta tgctgaattt tactttcaaa
    21361 ttactgtgga gcagtttgat ttgtcaacca agcaatgtac tttcgggcat ttacattatg
    21421 ttgttctagt gtagtagcaa attcatgata accaaaatta gttacactgg cacagaaata
    21481 gagaaaatca tgctgctccg gattcaaaac ggcttccacc gcattaacat caggcataaa
    21541 aataggtccg ggaggcaatc ctccatattt ataagtatta taaggggaat ccgtaattaa
    21601 atgtttatta accactcttt taatcacttg cgtgaaatca tctgtctctt ttttataggc
    21661 ataaattacc gtaggatcgg cttgtaaaag cattcctttt tttaaccgat tcaaataaac
    21721 ttgtgctact cggggacgtt cgctaacctt tgcggtttcc ttttgaacaa tggaagctaa
    21781 aatagaaacc tctatcgggg taagtccttg aagtgctgcc atcgcttttc gctcttcatt
    21841 ccaaaaacga tagtattctt tggtaaattg atttcttagc ttttcagtgg aagtattcca
    21901 ataaaattca tacgtattgg gcagaaaaag tgtaaaaaca gtttcttcgg taaaaccgtt
    21961 ttccttcaaa aaggcagggt ctttaaataa gtttaacact tccaaactat ccagttccaa
    22021 ctgtgaaccc aatcgtccgg caaaatcttc caatcgttcc tggttattaa aagtcacttt
    22081 caccggcaga tttaaacgca aagcagtaat aatcttgtta ttattcatgc cttgggttat
    22141 ttcaaatttt cctgctttga tattattaga gtaggacctt ctttcggcta aagaaacgaa
    22201 cgtacccatg tcttttatat agggcgaaag aatagtttga acttcttcaa acgtagcatc
    22261 agtaggaatg taaacagcaa ctttttcttc tgaaaattga gtattgggtg taaaaataat
    22321 ttggtaaacc ttaaaaccaa aataggccac tacagcaaga acagccaacg aaatataaag
    22381 aatgattttt ttgagtttca taagtaataa gaatagtata attgcaaaat tatcgaatta
    22441 attggtaaag tgcttcatct gaaaaaatgc catgattgaa attccaatct tttttgattc
    22501 ccacacagtg aaatccaaaa gtagtaaaaa gagaaatact ggcttgatta gtggtcgcaa
    22561 tagtagcaaa caattgatgt acaaataaat gctcaaaagc atagtgaata agcaattcta
    22621 atgcctgatg accataacct tgatacctat tctcagggtt ttgtatcaaa atacctatcc
    22681 ctgcccgttg atttttagga tcgtaatcgt acaagtcaat taaaccaata gcttcaaagg
    22741 aatcattctt acaaatcgct aaacgcaatt gcttcgcttc ataaatatct tgatgagcat
    22801 tatctaaata ttgccgaatt aaaaatttgc tgtaaggtgt ttgtgtctga ctgatttccc
    22861 aaaccgaggt atcattttct aacgcataga caaattctaa atcttccggt tctaatgctc
    22921 gtaaataaat atgttgtcct tgaagtgaat tcatttttat agggttgcta agtcaatttc
    22981 tccactaaat acaaacgtag ccggtccggt tagaaacaca tgagtgtaat ttctatcctc
    23041 tttttcaaaa cgtacctgaa gtgatccgcc ttctaccaat aacgaaatgt cgttggaagt
    23101 cgttttgcca atcacattca tcgcaatagc aactgcagtt acgccggttc cacaagacaa
    23161 ggtttcatct tctactcctc gctcataggt gcgaacagca aaaagtgttt cggataaagg
    23221 ttctacaaaa tttacattac ttccggcttg tccatacaaa tcactgtacc gaatttggct
    23281 tccaagttct ttcacgggaa ccgattttac atcatcgact aaagtaacat gatgtggcga
    23341 accggtattc ataaaaacgt aatcggaggc tacttgaata gttgtaacat ctttcatctg
    23401 taacgaaact aaaccagcgg ttgtaaattt ggcaaaatgc tccccgtcaa ctgctataaa
    23461 ggtggtttct tgttgaataa ttcccaattg ttcagcaaaa gcaacaatac atcttccgcc
    23521 attcccacac atggaacttt cgtttccgtc tgaattataa taaaccatac gaaaatctgc
    23581 attttgatca ttttccagta aaatcaaacc atcggctcct attccaaaac ggcgatcaca
    23641 caacttttcg ataagattag tctgcttttt tggaaaattc tccaaacggt tatctaccat
    23701 gataaaatca tttccagtcc cttgatattt gaaaaattga atcttcattt ttatttttgt
    23761 gtctcacaaa agtacaatta ttttaaagaa aagttaaaga ttgttaaggt tcgttaaacc
    23821 tattttaaaa aaacaataaa tcgtataatt ttacatttgt ataactaata atagatgtaa
    23881 acaatgaaaa atgcaaccaa tttattttta gtatccttat taagtggcat tacgacaata
    23941 ggggcttaca aattatttat cgaagataca acttcttcaa aaaacatcat tacaacagct
    24001 aatgctttat ctgccaaaac tgtcgggctt tctcccgaaa caattgattt tacagaagct
    24061 gccgaaacag cattacacca agttgtccac gttaaaaacg tatcttatcg taccgtttcc
    24121 aatccaatta tggaattcat gtatggctac aaaggaggac aacaacaaca gcaaattgga
    24181 acaggttcag gggtaattat ttcagaagat ggctatatcg tcacgaataa ccacgtgatt
    24241 aatgaagccc aggatattga aatcacccta aacaataaaa aaacatacaa agccaaatta
    24301 ataggaactg attctaaaat ggacatagct ttattaaaag tagatgccga agaaaagtta
    24361 ccgtatgccg tttttgctga ttctgatcaa gtgaaagtag gcgaatgggt attagcggtt
    24421 ggaaatccat ataatctcaa ttcaacggtt acagctggaa ttgtttcggc aaaagccaga
    24481 aatttagaca ctggtggaat tcaatctttt attcaaactg atgcggtggt gaatcccgga
    24541 aacagcggag gggcattggt aaacacacag ggtgaactaa tcggaattaa tacgatgatt
    24601 tcatctaata ccggtagtta cgtgggctac tcgtttgccg taccttcaaa cataacccgg
    24661 aaaattattg aagacatcat ggaatttgga aatgttcaac gtggcatttt aggtattgaa
    24721 ggatatgaat taaacggaaa tgcgtctaaa gaattaaacc tttcagaaac acaaggtttt
    24781 tatgtaaata aggtaacaaa aaattcggga gcggaaaaag caggaataaa aacgggagat
    24841 gtaataacta aattggacaa tcaaaacatt acctcctttg ctgaactttc aggatatgta
    24901 aatacaaaac gaccaaatga tgaaattaaa gtagcggtca atcgtgaggg aaaatcactg
    24961 attataccgg tgaaattaac caaaaaagaa ctcattaact acgaatttta tgggttggaa
    25021 atagaagatt tagacgctgc tgacaaaaaa caattcggca ttaaaagcgg cgtaaaaatt
    25081 aaaaatatta caaatgaaaa actggttcct tacagtgaag aattaaaagg aagtatcatt
    25141 ttaagcatag atggcataaa agcgactgat gtggaaagca tctccagaaa tttgggtagt
    25201 aaatctgaaa aacaaggtgt acgagtagaa ctgatgctta aaaacggaca agtaatgaga
    25261 atcataatat aagttgtata gttagtttgg ggtaaatcct gcaaagtcta cggttggact
    25321 ttgcaggatt ttttattttt aaaaaaacca aaaaaaagtt tacgaaaacg tttgaaatct
    25381 atacttttgc accaaaataa aacaacacta accaattaaa acgcatgaaa agtaatccat
    25441 ctttatacga aaaagaactt tcgtttcaag cagaccgtcg cagagccgga gttgaattta
    25501 tcaaaatcat cagtgattta tggtatgaca aatccatcga agtggtcttg ttccgaaacc
    25561 aattaatcga caaaaatgta tctgaaattc taaacttgca tgaatatgcc ggtgaatttg
    25621 tgggtaaacc aatctctatt tttgattccg tggaaatcgc acgggaaatg ttgtcactag
    25681 acttaccacc atccaaactg gatattggaa aattaacttt cgagtaccac ttagaagatg
    25741 ataaatacca taacaccaaa tcatttgtaa tagataaatt acgcaaagcc aaagatgttg
    25801 acagcattaa accaaaagat gttgtgttat atggctttgg tagaatcgga cgattattag
    25861 cccgggaatt aatgtctaaa accggaaaag gaaaccaatt acgcttgcgt gccattgtaa
    25921 cccgtgataa aaatgatgcg acaattttag aaaaaagagc ttctttatta cggtatgact
    25981 cagtgcatgg cgattttcaa ggttctgtga gtgccgacgt tgaaaataat gcattaatta
    26041 tcaacggtac taccgtacac atcattacgg ccaatggtcc ggaagaaata gactatacaa
    26101 aatatggcat taaagatgct ttagttattg acaacaccgg tgcttttacc aacgaagaag
    26161 ccttaaaaag acatctggtt tcaaaaggag tctccaaagt gctattgaca gctcccggaa
    26221 aaggagttcc aaatattgta tatggcgtaa accataacga ccataatccg gataaaatag
    26281 atattttttc agcagcttcc tgcacaacaa atgccattac acctattttg aaagcggtgg
    26341 aagatacgtt gggagttgta aaaggtcatt tagaaacgat acatgcttat accaacgacc
    26401 aaaacttagt ggataatatg cataaaaaat accgcagagg tcgtgctgcc gcattaaaca
    26461 tggtgattac cgaaaccgga gcaggaagtg cagtggcaaa agcattacct tcactggccg
    26521 gaaaattaac atcgaatgca atacgggtgc cggttccaaa tggttcacta gtcgtattaa
    26581 atttagaagt gggcaaggaa acttcaatcg ctgaagtcaa cgaaataatg aaaaaatatg
    26641 ccttggaagg ggaattagtg gaacaaataa aatattcttt gagcaacgaa ttagtttctt
    26701 cagacattgt gggcaccaat gcaccttcca tttacgacag taacgctact attgtatcag
    26761 gcgacgggaa aaatattgta ctttatgtgt ggtacgacaa tgagtttgga tacagccatc
    26821 aagtaattcg attagctaaa tacattgcta aagtaagacg gtatacatac tattaatagt
    26881 taataatcaa aatattatca tccgttttat gtttcatgaa acggattttt ttatctttag
    26941 attctaatta tagcatgatg aactattttt atttatgttt tttgttgatt ttaccctgtt
    27001 cgggaatttg tcaaatacaa ccgactccaa aatcatatca taaaattaaa tttttatacc
    27061 aaaaaaaatc aaccctacga ttggatgaaa aaggagtata tgcagacacc cttcaactga
    27121 aaaaaatgtt tccggatgtt gaatttgaaa aaataaagct ctccgaagaa aataataata
    27181 cggtagttgg cttcgttcca attgaaaaat tgaatgggat tcataaaaac gaaattttga
    27241 atcatctttc ctccaataat tgcctgattt atggaacggc ctatcccaaa aaagacctga
    27301 ttgattttac tgtcgattat aattcaccgg aaactgctct ggcttattat ttattcggaa
    27361 aaacacaaac tcaattcagc catacgtatt cgtatactta tttagaaaaa aatgcacggc
    27421 tagaaattag ttcttcagga actaaacaag cagtagcaat aactaaaaat caattgacct
    27481 ggataaacaa ggaccaactg actggaacat tttcagtaac cgaaaaacct aataccaaaa
    27541 aaaatcaatt aattcaaata gagaaaaatt taccccgttg gatttcgcca attccttttt
    27601 ttgaaaatgt tcaaatgggc ataaaatcaa tttatgatgt agaatatgac tacctactga
    27661 ttactgtttc gtatgaataa tggtttttta aacgctatac cacaatagtt tatacttttt
    27721 agagttatag gtaagtactc taaaagcgta caaataacag atttacccaa aatcgtacat
    27781 gaaaaaccta ctccttctgg tgatattcac cacacatttg tcttttgctc aagacaaagt
    27841 ttccacttat aaatttggcc aaaatggcat ggaactgatt gctcgttcac catccggaac
    27901 tgttatcatt agtacattca atgcaaaaat gaccattcga caagacattg ctcgaaaaat
    27961 ttatgacttg tacaaagacc acaaaatctc tgggaatact aggctaactg tatatggcaa
    28021 agaggctaat gtaatgggaa aatgttacat aaaaaccaaa aacaacctaa catcggttga
    28081 attttattac gaaaaagtga gttggaataa cggtttggta gaggtatatg aaaacataaa
    28141 aacttcacat gaagtagtgg caatgaaaac aaattaaagt ttataaaaaa caaactcccg
    28201 ataattcggg agttttctat gttaagaaac gttgaattaa gctttttcgg ctgcatgaac
    28261 tggctataaa ctggctctgc aaaccgaaat agatttagca aatttttatt aatcaagaaa
    28321 acaaggtcca attcaaatga tactttacat ttctaccatc acctgctctt atcaaagctc
    28381 caatttcaaa taaaaattgt aaatctcggg ttgccgttgc ttttgaagtt ttggtgattg
    28441 aaatgtattt cttggctgtc attcctcctt caaaacctga cttccctttt tcagtcattt
    28501 tttgcaagac ttttgtttga cgttcattca attgatgatg ataacgttta aagaataggg
    28561 ccttttcaac aacatacagc actgtttctt ttacttcttt ttgtgcttca attaaaattg
    28621 taaaaaaata acgtagccaa tcactaacat cttgttttcg ttgtgcttgt tttagtgtct
    28681 cataataggc ttgtttgttt tcttcaattt tcttggaaag actaactaaa attggctttt
    28741 caagtgtttc tgccaatgct ttttcagcta aagctctccc tattcgtcca tttccatctt
    28801 caaagggatg taatgtttca aaatacaaat gaactaatgc ggaacgtaac atagctctcc
    28861 caatttgacc aatttcatgg taaggaaaat ggtgatacca tttttcaaaa ccagttaaaa
    28921 ggtttactaa ttcagtagat ggtggtgctt caaaatgcac ttcaatcttc cctgttctac
    28981 cggaaataat ttgcatcggc tctgtgctgc ttcgccattc tccctctttc accgttttgt
    29041 caaacttcat taacgtgcga tgccattcct ttaacatagc taaggagaaa gaatgttgat
    29101 aatcctttcg aacctgcaac atcaatgttg aaatggcttg tgctctttta tcttttgaaa
    29161 caataggtgc atctaaaata cctaattgtt tttgcaaaga ggacatgaca gctgctcgac
    29221 taaagtattc gccctcaatt tcagatgttt ttaaagcttc cgacaaaaca acttgtagca
    29281 atagttcgct tttatcttct tcggatacat ttacaaacaa tccgttaact tcgccaaatt
    29341 ctgtggcaaa ttcttggatt tgacttataa aatcatctac tttaaattga aaattaggaa
    29401 aatcagtctg ttgccaaaaa taatccatga gccgtttgtt tttactattc ggctcaaata
    29461 tacaaaaaaa atgagccgat taacggaatt aatcggctca aaataacaaa tctataactc
    29521 agctaacagc tgctgaattt gcttaattta atgtatcaag aaaacgaatt gaaaacatga
    29581 ccattttttt tactgtttta aacccgttaa gcttaagtaa tacaaaactg attttttaaa
    29641 aataggtgat ttcacctttc aaacaaattc aaaagtgagt atcaactcaa ttattttgca
    29701 tagccatgat tttctgtact aaactaaaca atctgcgaat ctgcagctaa aattacctat
    29761 aaccattaat aaaaaggtta tgaatctaat accttaatga ttaaaaaata tacaatttta
    29821 caatcttaaa cagtgtaatt tgttgctgaa attataaaag aggataaaga gtatagctaa
    29881 tttaatataa caaaaaactc ccgaaaaaat cgggagttta aatattgaag tatcttgtac
    29941 aattaagctt ttccggcggc aattaaattc aatgcagaac cggctacaaa ccaacctatt
    30001 tgactttcgt tataggtatg attggctaaa atgatatcct tcgttccatc agcatgaaca
    30061 aactcaaggc ttaatggttt acccggagca aattccgtta agtccaaaaa gttaatggta
    30121 tcattttcct gaattttatc ataatccgct tcgttggcaa aagttaaagc taacatacct
    30181 tgttttttca agtttgtttc atgaatacgg gcaaatgatt ttaccaatac cgcttttaca
    30241 cctaagaaac ggggttccat agccgcatgt tcacgtgaag atccttcacc gtaattgtga
    30301 tcacccacaa caatcgaagg aactccggct gctttgtaag cccgttgaac agctggtaca
    30361 gcatcataag ttccggttaa ttgattttta actttgttag cttcctgatt gaacgcatta
    30421 acagctccaa tcaacatatt attagatata ttatccaaat gaccacggaa acgcaaccaa
    30481 ggtccggcca tagaaatatg gtctgtcgta cattttccaa aagctttgat taacaatttt
    30541 gctccggtga tgttatttcc atcccatgcc tcaaaaggag caagcaattg caaacggtcg
    30601 gaagttggcg aaacagctac ttgaacacta cttccatctt ccgcaggggc ttgaaaaccg
    30661 gcatcctcta cgtcaaatcc tcttttaggt aattcatcac caaaaggagt tttcaacatt
    30721 acttcttctc ctttatcgtt gattaaagca tccgtaagcg gattaaaatc caatctgcct
    30781 gaaatagcta aagcagcaac catttccggt gaagttacaa aagcatgtgt attggggttt
    30841 ccatcggcac gcttagaaaa gtttctgtta aacgaatgca caatcgtgtt tttttctcct
    30901 ttatctgctc cttctctgtc ccattgacca atacaaggtc cacaggcatt ggtaaatact
    30961 tttgtaccca ttttttcgaa agtggcaata attccgtctc gttcaatggt aaatctaatt
    31021 tgttcggaac ccggattaat accgaattca gctttaggcg taataccatt atctaccgct
    31081 tgttgaacga tagaagcagc tctcgccata tcttcataag aggaattggt acaagaaccg
    31141 attaatcccc attctacttt taaaggccat ccattagcag cagcttcctc tttcatttta
    31201 gaaaccggag ttcctctgtc cggggtaaac ggtccgttaa tatagggctc caattctgaa
    31261 agattgattt cgattaattg gtcaaaataa cgttccggat tagcatatac ttcatcatct
    31321 gccgttaaat aagaagctac tttatctgct gcattcacaa catcttgacg accggtagcc
    31381 gctaaatatc ttctcatgga atcatcataa ccaaaagtgg aagtagtagc accaatttca
    31441 gcacccatat tacaaatggt tccttttcct gtacaagaca tagacgtggc tccttcgcca
    31501 aaatattcaa cgatggctcc tgttcctcct tttacggtta aaatatcggc aacttttaaa
    31561 ataacatctt ttggtgcagt ccaacctgat aatttaccgg ttaattttac acctattaat
    31621 ttggggaatt ttaattccca agacataccg gacattacat caacggcatc agctccacca
    31681 acaccgatgg ctaacatacc taatcctcct gcattaaccg tatgagaatc cgttcctatc
    31741 atcattcctc ccggaaaagc ataattttct aaaacaatct gatgaataat accggagccc
    31801 ggtttccaaa aaccgattcc gtatttgttc gaaacggaag aaagaaaatc aaaaacttct
    31861 tttgattgtg aattagctaa agctaaatct gatttcgcac catttttagc aagaattaag
    31921 tggtcacaat gtaccgtggt gggtacagct acagtctttt ttccggcatg cataaattgc
    31981 aataacgcca tttgagccgt tgcatcttga caagctaccc tatccggagc aaaatctaca
    32041 taatcttttc cacgggtaaa agcggtggtg ggatttccgt cccaaaggtg agaatataaa
    32101 atcttttcag ataacgtaag tggacgacca actaactcac gagctttatc tacacgagta
    32161 gccatggttg catacacttt ttcaatcatt ttaatatcaa aagccatggt ataaaaggtt
    32221 tattttgttt gtgatgcgaa tttacgaaaa cgttagaaca ttggaaaatt aatagcaaaa
    32281 tatatctttt ggaattgatt ttttacagaa aagttatttt ttgaatctta ttttcaataa
    32341 aagctaaaat aagggcataa aaatgcaaat ttgacaattt tagagcacaa attacttgtc
    32401 ccgtaaatcc gtcatcggct tttcgtaaaa tttatacaat agcgtgctta agaaaaaggt
    32461 tataattaaa taaataaaag tataaaaatg caattgatgg agagataaac cctccgtgtt
    32521 aaagtaatgt ttcataagtt gtaaaacgac accataatgc aataaataaa ctgaatagga
    32581 aatgacgctg ataaaaacaa ttggttttcg gataatagca aagtgaactg ttttccattc
    32641 cgctaaaaaa ggaagaaaca aagcaaacgt taaagaggtc aaaggcaaat aaaaaatagc
    32701 ccaaaaaaga ggaaatgaag caaacgtaag ttgaaaatag ccaactccgg caaataaaaa
    32761 tccaaataaa aacaagccca aaacagccat aacaaactta ccctttttcc aaaaagcagt
    32821 aaaatttaaa ctgatccaac taaatagaac ccctaaaaaa atggcatcca accgataaat
    32881 aaccaccgct tttaaaccaa ggttccattg attgatatct gaaggtggtg tggtgaaagc
    32941 ataccagact ttggtgaaga aaaaaaagaa aataaaccct ataactacat ataaaaaaaa
    33001 acgggatttg ttaatcggtt taactagtcg cgtttttaaa aacaaggcaa ttggaaaaag
    33061 caaataggca aactcttcta cggaaagact ccaggattcc gtaaaaaaag gaagcatggg
    33121 tgaagcaaaa ttttgcaaaa agaaaaaata tttccaaccc tcctgaatcg aataaccaac
    33181 aataaaacta ataagaatag taattaataa aactagataa taattgggca acgttcgaaa
    33241 ccaacgtcgt tttaaaaaat ataaaacact tgaaatagta aaatcatcct gtaaataaag
    33301 tcggtaaaca attcggccaa tcaaaaatcc gctgagtaca aaaaaaattt caacgcctaa
    33361 ataaccaaac aactgaaaaa cttgcgaaat aatgccatta tctggcggat aaatccacaa
    33421 taaatgagaa ctcaacacca ttaaaatagc aaccgctcgc atcaaatcta atccgaatat
    33481 tcgtttttca gaatggaggt gtaaaagaga cattaaatga tgtatttttg aattaaaatt
    33541 cggcaattgg ctaaaccaaa gatactaaaa atcacattac ttttaatagt gctctccttg
    33601 gcaggcgggg tcatttggta tgcccaatta tctaatcggc acaaagcgat tgtgaaaact
    33661 acttttttac acaaaacggg attagttgat agtgattgga aaatcgaaaa agcgaataaa
    33721 gaatacaaaa tgctgtcgcc cacatttatt gtagacaaca tctacaaatc aatggagggg
    33781 ccaaaagcaa ccaatacagt aatgctgtca caagatgcta ctttgttgtg gattaccgga
    33841 tttaaagtgg ttgctcaaga cgctgataca gaaaaaagga tatcaaatga ttttatttgc
    33901 cacatgaatg tggatattaa tgatgcaaaa tattactccc attttggatt agaaaaccgc
    33961 attggcaaac aataccctag actgacttcc ctttcacacg gaatggaatc attcgaattc
    34021 ccgaagggtt ttggcatccc catgaaagga aatgatttgc tgtttgtgac cacacaaacc
    34081 ttaaaccaca acctgaaaga ggtctataaa aaagtaaaac ataaagtaac tataaattat
    34141 caggacaagc cttcgctatt tcctttaatg agcaaaacag cttttatcat gcttccgtat
    34201 gatcaatatg acccatataa aagtccaaca gaccccggaa ctgatttttg tattccggtg
    34261 gaaaccaaaa accatagtta tgaagatgga aaaggaaata aactgtccgg tcattgggtg
    34321 attccaaaag ggaaaaacac ctatcggagc gacatcaacc accaattaga aatcaaagac
    34381 agccttcgtt tacatgccgc agcaatacac gtgcatccgt ttgccacaag aattatgctt
    34441 tacaacaaaa ccactcaaaa accggttttg gtgagcaaaa ttacgaacca taaaaacagt
    34501 attggactga ccaacattga aaatttttcc tctacagaag gaaaatggct ttatgcaaat
    34561 caggaatatg aaatagtact ggaagttaat aacacctcct tagaaaacca agacatgatg
    34621 ggaagtatgt tcctcttttt ttatgattca gaaatggacg aaatcctaaa aaaatcatcc
    34681 tcgcagtcag ctaaaccagc ttcgcaataa tcgcttccac actcagtcct tcagcgtccg
    34741 ctttgtagtt tttcacaaca cggtgacgca aaatcccttc agcaaccgct ttcacatctt
    34801 caatatcggg tgaaaattta ccattaaaag cagcattggc tttagcagct aatatcaggt
    34861 tttgagaagc tctgggacct gctccccaat cgatgtagtt tttaataaaa tctgttgcta
    34921 aattactgtc cggacgggtt ttagtaacca acgtaacagc atattcgata acattatcag
    34981 ctaccggaat acgacgaatc aaatgttgaa aatcaataat ttcctgagcc gtgaataaag
    35041 agtttataac cggcttatca tcagaagtgg tgctttttac aacttgaact tcctcggcaa
    35101 aagaaggata atctaattta atggaaaaca taaaacggtc taattgtgct tccggtaaag
    35161 gataggttcc ctcctgctca atcgggtttt gggtggccaa aacaaaataa ggcaaactca
    35221 atttataatt ttgtccggca atggtcacgg cacgctcttg catcgcttct agcaatgcag
    35281 cttgtgtttt tggcggcgta cggttgatct catccgctaa aataatattg gaaaaaatgg
    35341 gccctttgat aaacttaaac gttctgtttt cgtctaaaat ttcactccct aaaatatcag
    35401 aaggcatcaa atcgggggta aattgaatac gtttaaaatc caaccctaat gcttgtgcaa
    35461 tagtattaac cattaatgtt ttcgccaaac cgggcacacc aaccaaaaga gcatgtccgc
    35521 cggaaaaaat actcaagagt acttgatcaa ctactaaatc ttgtccaaca ataatttttg
    35581 aaatttcttt ttttaacgcc aatctttttt ggaccagggt ttgtatagcg gttacatctg
    35641 acataatggt tgtggataat gggttattgg taataggcaa aaacgcttta tttttttagc
    35701 caattattag taaaatcgca atcgcggtat tccgcattaa tacggatgta ggtttctaat
    35761 attttttcgt tagaccattt tccgatagct tcaaattgtt tttcctttaa agctaactct
    35821 ttaatcttaa cataatccgt agcataatca gccacgtggg aatcaattcg attagtcacc
    35881 atcaataatt tatatttctt catccctttt tgatcttcat ctaaaacagg ttgcgaaact
    35941 tcatttggct ttaaactgga aacttgacta tataaacttg gatccatttt agttaactca
    36001 aaacgggtat ccaatgtttt cggattcagc aaaatacccc cattggtttt agtttctttt
    36061 tcatcggata aactacgggc tgcctcagca aaagttaatt cgcctgaaac aatccgactt
    36121 ctgattaagg ttactttttc tttcgcctct cgtaagtcgt catccgtgat ttttggtgtc
    36181 aaaagaatat gacgcaattc aacctcctgt cctttaattt tttctaccaa aataatatgg
    36241 tacccaaaat cagtttgaaa aggcatcgat atttctcctt catttaaact aaaggcaatg
    36301 tctttaaatt ctttgacaaa gggtgttttt cgattcattt tataaaaacc accggaggca
    36361 cgggaacctg gatcttgtga atacaatacc gctcgtgttc tgaaactgga accgttttct
    36421 aaaacgtctt tcctgatttc tattaactta tcaattactt tttgtctttc tacatcactt
    36481 actttgggtt caatcacaat ttgagataac tccaattccg ctccaaaaac aggcaactct
    36541 tccgttggga ttttcttaaa aaaagttctc acttcttctg gcgtaatctc tacatccgct
    36601 accactttat tacgcatttc agaagataac tttccttgct ttaaaacatc aaaaaagaag
    36661 gaacgaaatt cttcttcatt tttcttgtta tagaatttta gcattttatc catcgacccg
    36721 atttgctcta acatataggt cagccgttcg tccatcatgt tttgtatttc accatcattc
    36781 accacaatgg tactgtcttg taacgcttga tgtgcataca aacggtcttc catcaacttt
    36841 cctaataatt ggcatctggt gatatcatta acggaaatac cttggctggt aagctcaata
    36901 tattccatgt caatatctga atctaaaata atgaaatccc ctatcgtggc aataacacca
    36961 tccacttttt tgcgttggct tactttttta actgtatctt ttttaacagc agcctcctcc
    37021 tgaattactt cttgtgctgt tgcaaaaaca gtaaaaaaag cgaaagcaaa cagtaggaga
    37081 aacttgttat ttataaattt catagttttt atttttaatg gcatcatcgg taatttcttt
    37141 ctcaaatttt ttaattaatt ctaattttct tctgtttatg attaactctt ttagagtaga
    37201 tttcacatat tcatagggac tcacctcatt tttatcaatt acattactaa tcttcaccaa
    37261 gtaaacatcc gttgaatccg gatattgaat cgtttttccc ggagcaatat aattttcaga
    37321 gttatccgga gtaataaagg gcaatttttg ataaacctga ttcatttcta cccaaacact
    37381 gtcattcagg gcagaacctt taaattgcaa ttgataggtt tcccaaaatt tagcatcact
    37441 ttttttggta tcaaaaaact tactcttaat caaatcgaat ttcggatgct ctttagacaa
    37501 atgaatatac cgcaactgaa ccaaggctcc atttgttctg aaattctctt tattctcttg
    37561 ataataagct ttaatgtcag cattggaaac tgccgtatct acttctcttt taacaatggc
    37621 ttctaaatag gctttggtat ataaatcaac tttgtactgc tgaatcagtt tatcaaatgc
    37681 tatttgctct tcttttgtaa tatttaattc agcggcatta atcaacaact tttgagtagc
    37741 ccaacggtta atgaaatttt gtacaataag cagactgtct tctttcgtag tcccttccgg
    37801 caataaatcg gctatgtcac ttttatacaa ataactttct ccaactctgg caatggcttc
    37861 gggttttgct tcgggtttaa aataagaaca cgaaacaaaa acaaaaaaca caagcacgat
    37921 gccaaggggt ttcaacatta gtttttaatt tgctttttaa cactgtcaaa cacactttga
    37981 ttaacagtaa cgttgaattc ttgcttcaaa ttagtaaccc aattttcttc caaatattgt
    38041 tgatagtcat tgattaccct gcctttacat tctgctaatg tttttggtcc ggcttcttga
    38101 acggcattga cttgaacaac atagaaataa tcttcttcat gaactaccgg ttgtaatcct
    38161 ttttgatagg atactgtttt gggcaaaata gcattatctt cctcaaaaat tccggcagtt
    38221 gtcatcacat taacaacatc tttcgtattc aattcctttt taatttcctc aactgatttt
    38281 ccttgtttaa gcaatttatg tgcttttttt gcaatatctg ctttggtaga ggaaataatc
    38341 aacacatcat atcttttttt ccaaaggtat ttattagcat tctctgtgtg aaacgcttgt
    38401 aaaccaagtg tgtctgtttt agatttttcc caaatctctt tctccattaa atcaaacaac
    38461 aataatccgt cacggtattc atccatcaca gcggcaaatt caggaaattc attttctaaa
    38521 ttgtcattgt aataggtatt taattccgca tcaacaaaat tagaataatg aaaatcaacc
    38581 aatttagcaa caggtctcac ttgggcaggg tttttttgtt gggaatacaa ataatttaaa
    38641 aactgttccc cactcacgtt ttgattagca aaagtgaaca agggagctgt aaagtctttc
    38701 atgctctcag gtaatttcca agtagagtca taaaaactgt cattcaccgt ctttttaact
    38761 ttagtaaaca tcgcatccga acgtttaacc ggatacttag ctctcaattt ttcattcagc
    38821 gactcagtga tttttctgga acgctcatca cgaccaattt tagtttcaat atctggcttt
    38881 gcctcttcat acgttttgac aggatgcttt tcaatcaatt tcataatatg ccatccaaac
    38941 tgagtttgaa aaggtttcga aatatcagta ggattttgaa gagaaaaagc aacggtttca
    39001 aactcttctg aactcaattg accggaaccg aaacgattta aaacacctcc ttttgaggcg
    39061 gaagatttgt cttgtgaaaa ctgctttgct aaggcttcaa acgattctcc ttgctgaatt
    39121 ttattgtaaa tttcaaaaat ggtagtttta tttttctcca gttctgtcgg actgtcatcc
    39181 gtaggattca tcaacataat gtgagcaacc gtaacatcgc cacgattatc ccgaatgtct
    39241 tttacttcta tgatatggta accgaaacgg gttcgaacag gttgcgaaac ggctcccttg
    39301 ggtgtagtaa atgcagccgt ctcaaaagga taaaccatac ggaaagcaga aaaataacct
    39361 aaatcacctc tgttttcttt agcactcggg tcttgagaat attgaaaggc caattgaccg
    39421 aaatcttccc cttttaaaac cctgtttcgc aattctataa tacttttata ggcagctaac
    39481 gtatcttccg gcttagcatt ttcgtctacg gttagcaaaa tatgagccgc tcgaacttct
    39541 ttcaaggaac gggcatagcc ttcatcaatt aattctttcg taacttttga atcagtcaaa
    39601 taatttttag aaagctgtgt acgataggat tttaattcat tttgatattg cggattattt
    39661 tgcaagccca aagcattagc tttagtaact ttcaatttgt accctacaaa taattctaaa
    39721 taatgatcca aatctttctg cgattcatct ttaaccaagt ctaagttttt attataaacc
    39781 ctaataaatt catcagtata ataaggttta tcattaatcg taaaaagcac ttggttagaa
    39841 gattgggctg aaagtaatgc cgtcacaaaa aaggcaaatc ccaaacaaaa ttgtttaaca
    39901 gtcattttta aatgatttaa aagaatgatt tttataatag gcaacaaatt atagctgttg
    39961 caactcacaa aattaacaat tcaaagacat taaacaagta tagcccctac tattaacaaa
    40021 aatttattaa cgtaaaatgt ttatactttt ttgactaaga aatactattt ttgcacacct
    40081 ttaaaaagtg ttatgagttt tatagaagaa atcaatagaa gaagaacttt tggtattatc
    40141 tcgcatcccg atgccggtaa aacaacctta accgaaaagc tgttgctttt tggaggagca
    40201 atccaggaag cgggtgccgt aaaaaataac aaaatcaaaa aagcagcaac ttccgacttt
    40261 atggaaattg agcgacaaag aggtatttcg gtggccactt ctgttttggc tttcaattat
    40321 aaagacaaaa aaatcaacat tttagatacg ccaggacaca aagactttgc ggaagacacc
    40381 ttcagaacgt taacggctgt tgacagtgtg attgtagtag tggatgtggc aaaaggagtc
    40441 gaagaacaaa ctgaaaaatt ggtggaggtt tgtcgcatgc gaaacattcc aatgattgtt
    40501 ttcatcaata aattagaccg tgaaggaaaa gatgcctttg acctaatgga tgaagtggaa
    40561 caaaagttga aattaaaagt tactcctttg agtttcccta tcggaatggg ttatgatttt
    40621 caggggattt ataacctttg ggaaaaaaac atcaacttgt ttagtgggga tagccgaaaa
    40681 aatattgaag ataccatcgc tttttccgat ttaaacaatc cggaattgga taaaattatt
    40741 ggtcaaaaag cggctgacaa actcagagaa gagttagaat taatcggtga agtctatcct
    40801 acttttgata gaaaagaata tttagaagga catttacaac cggtgttctt tggttctgcc
    40861 ttaaataatt ttggtgtgag ggaattattg gattgcttta ttgacattgc cccctcgcct
    40921 aaagcgaaac attctgatac gcggttagta gaaccaaccg aaactaaaat gtcaggcttt
    40981 gtttttaaaa tccatgcgaa catggaccct aaacacagag accgtttagc ctttgtgaaa
    41041 attgtttcag gaacgtttga acgtaacaaa ccctattcac acgtgcgttt gaataaaagt
    41101 ttaaaatttt ccagcccgaa tgcttttttt gcggagaaaa aagaaattgt agatgtctcc
    41161 tatccggggg atattgtcgg tcttcatgat acaggtaact ttaaaattgg cgatacctta
    41221 acggaaggcg aaataatgaa tttcaaaggg gttccgagtt tttcaccgga acatttcaga
    41281 tacatcaata atgccgaccc gatgaaagcg aaacaactgg aaaaaggggt agaccaattg
    41341 atggacgaag gggttgccca gctttttacc ttggaaatga acaatagaaa aataattgga
    41401 accgttggtg ctttacaata tgaagtaata caataccgct tggaacacga atacggtgcc
    41461 aaatgtacgt atgaaaattt tccggctcac aaagcctgtt gggtaaaacc ggaggatcct
    41521 aaaaatgaag aatttgcaga attcaaacgg attaaacaaa aattcttagc ccatgacaaa
    41581 tatggtcaat tggttttttt agcagattcg gaattcacga tacagatgac acaaagcaaa
    41641 tttccttcgg tcaaactttt ctttacctct gaatttgaat agcaactcat tctaaaataa
    41701 atcattgtaa ctgttatttt actattaaat atgctaaaaa gtatgatttt tacttttatt
    41761 tttaaaaatg attactatct ttgccgctga ttccatttga aatatgaaaa atttatataa
    41821 actttacgtc ctcgctttta ttttattatc tgacctaatg ttgtttgccc aaccgggaga
    41881 tgatgatgga acaaatggtg gattagaagg tgatgatata ccggccgctc caattaatgg
    41941 aaaattaata tggctggctt tagtaggaat agtatttgtc ttttattact tcaatacaaa
    42001 aagacaaaaa gcgtaatagt taaccccttt caggggtttt tttatacctt ataatttctc
    42061 atacaaatag tttgattttc ttttccaaaa aaaaaccaaa gctcaaagaa ttaattcctg
    42121 ataatttcgt ggatattcat tcccatttaa ttccgggaat tgatgatgga tcaaaaagct
    42181 tggaaaatac gtcagaaatg atgaatagct taatatcatt aggctttgaa caattcatta
    42241 caacaccgca catcatccaa aatgtttgga acaacaaccg agaaattatt caacagggag
    42301 aaattgcagt gatagaatcg ctgtcaagta ataaccaaga aataccattc aaagctgccg
    42361 cagaatacat gctggacact tccttttcag aacttttaca aaaagaaaaa ctactgacac
    42421 tgaaagaaaa ttatgtcctg gtggagatgt cttacctaaa tccccccatg caattagtcg
    42481 atttgattta tgaaacccaa ctggcaggat atattcccgt tttagctcat ccggaaaggt
    42541 atttgtttta ccataacaac acgagtgaat atgaaaaact taaaaaaatg ggttgtttgt
    42601 tccaaatgaa cttactctcg tctgtcggat actacggaga aggggttgct aaaacagcag
    42661 ataatttatt aaaaaaagga atgattgact ttgttggctc tgatgtacac caccaaaaac
    42721 acattgatgc ttttgccgga aatgtaatct taaaagaaat gaccccttta aaagaggcca
    42781 ttcaaaacaa tcagttattt cgatttcttt tgtaatattt ttttgtgcca aggcaattgt
    42841 tctggctctg aatctgctcc atagccatag ccatagccgt acccataccc atagccgtac
    42901 ccgtagccat aaccataagt tttggaagga tctgtaccat tcaaaatcat agccatgttt
    42961 ggtaatttat tctctttgtg caatgattta ggaataatca aagagtgttt tttcagataa
    43021 ttagctcgga caacatacac gaacacatca gcaaatttac taaccaacag tgtatctgtt
    43081 accaaactaa ccggggctgt atccacaaca atataatcgt attgcttttt caattcatcg
    43141 aaaaggtcac ttattttagt actattcaat aattcagcag g
//
LOCUS       DAOTCW010000004        43152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_422_length_43152_cov_48.278581, whole genome shotgun sequence.
ACCESSION   DAOTCW010000004 DAOTCW010000000
VERSION     DAOTCW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 43152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43152
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_422_length_43152_cov_48.278581"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..666)
                     /locus_tag="P5335_00600"
     CDS             complement(94..666)
                     /locus_tag="P5335_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73409.1"
                     /translation="MKFIYSLFILVLFYSCQKEPQKILASDYLNEEQVESFKYEIIRY
                     TDKLAKKATHETKFDSQFDSIYQVKAKNAELYHYFVDKKSNEIYFAIARIAPSLKVKK
                     VVTIGKLKKDAKGKIISYEEAFRTWKMEITELKTKSKFLFEKYIAGEDLTPYYTENAK
                     GEFYIEFPDSNTYYDNNKRKWVSIGITTIE"
     gene            complement(740..2146)
                     /locus_tag="P5335_00605"
     CDS             complement(740..2146)
                     /locus_tag="P5335_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73410.1"
                     /translation="MKKNLILKASMFIAVLAISMTSCSNDDNPIVVDVTPPVEEPNLP
                     VELVGDLATRTLTKDKKYLIKGQVFVRTGVVLTIEPGTIIFGDKATKGTLVIDKGGKL
                     IAEGTSVEPIVFTSALAAGTRDRGDWGGIVILGNAPVNQPDPAIEGITPAVIFGGTND
                     SDNSGILKYVRVEFAGIELTPNNETNSITMGGVGSGTQMDYCQVSFGGDDGFEWFGGS
                     VSSKHLVTFAMWDDCFDVDYGFTGKVQFGVSVRYGSYADQSGSNTFETDNGPNDNLTT
                     LITTGVFSNITGVGPRISNTQSINGNYQHSLDLRRRTALSIANSVFVGFPRGLRMNQQ
                     SVYDNYSSGLGVVTNNVMVAPTTTFSVGTGMTSTASDVQTWFNATNETITNTDLPAVY
                     LELGLNTNIFFGTNTSTGYASNPDFRVTSGTLTSGAAFTNPKLADAFFTPTTYRGAFG
                     STDWTDGWAEFNPVTKVY"
     gene            complement(2178..4982)
                     /locus_tag="P5335_00610"
     CDS             complement(2178..4982)
                     /locus_tag="P5335_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ73411.1"
                     /translation="MKIRLLIITLFITFFSTAQKGTVSGVLTDKDMNNEPLPFANALI
                     KGTSIGTTTDEKGFYSLSVEPGNYILQLSFLGYQTVEVPITVKAGQTVTVNKALTSGE
                     GVMLQDVVVVTSRRKNTEAAVMIEIKEAKQVVSAISAEQMSKGTDSNAADAIQRVPGV
                     TIVDGKFVMIRGLSERYNNVLINNAVAPSTEVDKRTFAFDLIPTKAMDKMVIIKTGSA
                     DKPGDFSGGIVNITTSENITEFNTFNVGFGYRMDTSFDTYYQSEGSKTDFLGFDYEFR
                     TLPSTFPSANLINTYPEVGVTASNTKLQNNFNPNKSTTLLDNNFGFAFGRSKKFANGM
                     NLQSVNMFGYSNTYLTYQRQFTRYTTLNEGETRPPQWLNYLDDYNQNEARVTVLSNWI
                     FKLDNDNTIKFKNLFNQIGENETIIRNGNNYLQRGEDLFRNYLLGYTSRSIYTGQLEG
                     IHQLNSTNSIDWVAGYNYMYQSEPDLRRFRTIKDVEDPNSVYEMIDPASTNLFDTGRY
                     FGTLSEYSVNNGANYTHNIERIKNDEELNPIKLKTGYYLDYRKRAFDSRYVSYFLPDY
                     GIDRITELKQLPLTDIFSPENVNTTDGWILKEGTRSIDSYDADNFLAAGYIYGEFPLN
                     KFDISAGVRVEHNILSLQSNTDLGPIDIENPVTSILPSLNVGYNISERALVRFAYSRT
                     VNRPEFREIAPFLFYDYENEAAKVGNAELKTAEIDNIDLRYEFYPNKGETMSLGAFYK
                     RFDNPIENVTQITTEQPQFNYANADSAYNYGVELEIRKSFKELTNNLYLGRLSANINA
                     SYIFSEVDLGGDVTSQAQVRALQGQSPYIVNVALGYTDETGFSANVIYNRFGDRIFSV
                     GDINFPTIYELSRNSLDLTFSKTFKKTTIKLGIQDLLNAKYQFYEDSNRDEKISKDID
                     NATSVFKKGTLFSLNLSHNF"
     gene            5164..5847
                     /locus_tag="P5335_00615"
     CDS             5164..5847
                     /locus_tag="P5335_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRZ73412.1"
                     /translation="MKKKDIKILLVDDEQDILEIVGYNLSQEGYQIVTATNGKEAIQK
                     AKKELPHLIIMDVMMPEMDGMEACENIRKIPELSNVIIAFLTARSEDYSQVAGFEAGA
                     DDYIAKPIKPKVLVSKVKALLRRLKEEDPKGDILTVGNIEVNREEYKIIQDGKEIVLP
                     RKEFELFYLLASKPGKVFKREEILDKVWGNEVIVGGRTIDVHIRKLREKIGDELFKTI
                     KGVGYKFEI"
     gene            5917..6960
                     /locus_tag="P5335_00620"
     CDS             5917..6960
                     /locus_tag="P5335_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRZ73413.1"
                     /translation="MAISFKKTYKFAVKSAFYITFFSMVFLTLLLYYFNQVSYVLITV
                     FTISIYLFSFFVVQYRVERFIYRRVKKIYDDVSLLDSTTFRSQPITTDMATLTKEVKK
                     FATDKKLEIETLKVREEYRREFLGNVSHELKTPLFTVQGYISTLLDGAANDKVLRKKY
                     LERAEKGVERLIYIVKDLDMITKLETGDLNLDYSIFDIVETIKNVFDLLEMRASKKKI
                     TLTFDTNYSKPIYVRADQEKIQQVITNLVMNSIKYGKEGGTTEVSIEDLVNKKIIIRI
                     TDNGEGIQKQHIPRLFERFYRVDKSGARSEGGSGLGLAIVKHIIEGHNEKIYVESQFG
                     VGSEFSFTLEKQK"
     gene            complement(6934..7998)
                     /locus_tag="P5335_00625"
     CDS             complement(6934..7998)
                     /locus_tag="P5335_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRZ73414.1"
                     /translation="METTHPKILVAPLNWGLGHATRCIPIIEALIEQGYEPIIASDGV
                     ALHILQKEFPQLTFLELPSYQIEYAKKGAFFKWKLIKNSPKMMEAIYQERKLVKKWVK
                     EYGISGIISDNRLGIYSKKVPSVFITHQLNVLTGNTSWITSKLHQYIIKKYDECWVPD
                     VAEIPNLTGKLGHLENPHFKINYIGPLSRFTKKELPKKYDLLIILSGPEPQRSILELH
                     LREEIKRYKGKVVFVRGVIEPEQKSEQTEHVTFYNFMTSVELEQTFNESEIVLCRSGY
                     TTVMDLAKLGKKAFFIPTPGQFEQEYLAKKYKKEGIVPYAKQEKFVIENLFEIDLYRG
                     LNTFKYDLDWKKLFLLFERE"
     gene            8085..8759
                     /gene="trmB"
                     /locus_tag="P5335_00630"
     CDS             8085..8759
                     /gene="trmB"
                     /locus_tag="P5335_00630"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRZ73415.1"
                     /translation="MGSKNKLKRFKENESFQNVIEPTREEVVSGDFKWKGNWNNDFFK
                     NEKPIVLELGCGKGEYSVGLAERYPDKNFIGIDIKGARFWRGAKTAVENGMHNVGFLR
                     TQIELVEFCFAENEVDEIWITFPDPQIKYKRTKHRMTNTEFLQRYKKVLKKDGLVHLK
                     TDSEFMHGYTLGLLHGEGHEVIYANHNVYKNEGAPEVVTAIQTFYEKQYLEINKAITY
                     IQFRIK"
     gene            8781..9416
                     /locus_tag="P5335_00635"
     CDS             8781..9416
                     /locus_tag="P5335_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRZ73416.1"
                     /translation="MNITLPILLGLSSAALGTTLPGLLNMTVAKVGMRDGKNRAVWFA
                     LGASVIVFFQTLIAVLFARFIDGRADIGALLQEIGFVIFTGLTFYFFWSGKKNKLKIK
                     KDEIKMRSKSSRFFMGVLLSSLNLFPIPYYVFISITLATYHYFYFDNYFIYAFSTGTA
                     VAAFLIFLGYISFFKKKDRSNSFLSKNINYVIGTITGLVALISLFKLFFKD"
     gene            9427..9753
                     /locus_tag="P5335_00640"
     CDS             9427..9753
                     /locus_tag="P5335_00640"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRZ73417.1"
                     /translation="MMEENFFERVYFIVRQIPYGRVTSYGAIAKALGTARSARMVGWA
                     MNASHHLEDVPAHRVVNRKGLLSGKHHFSGTNLMQQLLENEGIEVIDNQIIDFEKVFW
                     EPEISN"
     gene            9922..11064
                     /locus_tag="P5335_00645"
     CDS             9922..11064
                     /locus_tag="P5335_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRZ73418.1"
                     /translation="MKLDRKEILKALETISVAGEGKNMVESGAIQNVLTFGDEVIVDL
                     LLSTPALHIKKRAEVDIIKTIHELVYEKAKVKVNIKVETPEKPENPNLIKGKQIPGIS
                     NIVAIASGKGGVGKSTVTANLAVSLAKMGFSVGVLDADIYGPSMPIMFDVENAKPLSV
                     EVDGKSKMKPVESYEVKILSIGFFTKPDQAVIWRGPMAAKALNQMIFDADWGQLDFML
                     IDLPPGTGDIQLSIMQSLPITGAVVVSTPQAVALADAKKGVSMFMSEAINVPVLGIIE
                     NMAYFTPEELPNNKYYIFGKEGARNLAEDLKVPLLGEIPLVQSIREAGDYGRPVALQT
                     ATPLETAFENIARNIVQEVVNRNENLPPSEAIKITTMAGCSAVNKK"
     gene            11064..11303
                     /locus_tag="P5335_00650"
     CDS             11064..11303
                     /locus_tag="P5335_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRZ73419.1"
                     /translation="MTTEEIRLGIEKALDEIRPFLLSDGGNISLVDVLDSKHVRVRLE
                     GACVGCSVNQMTLKAGVETTIKKYVPQIETVENIA"
     gene            11414..12472
                     /locus_tag="P5335_00655"
     CDS             11414..12472
                     /locus_tag="P5335_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRZ73420.1"
                     /translation="MIQTDILIIGAGPTGLFAVFEAGLLKLKCHLIDALPQPGGQLAE
                     LYPKKPIYDIPGYPEVLAGDLVTNLMEQIKQFQPGFTLGETAETIDKLEDGSFIVTTN
                     KGTKHQAKAIAIAGGLGTFEPRKPLLKDIEFYEQNDRGVDYFVKDPEKYRGKKIVISG
                     GGDSALDWSIFLSDVASEVTLVHRRNEFRGALDSVEKVQELKNIGKIKLITPAEVIGF
                     KGTERIESVDVEINGARMNVATDYFIPLFGLTPKLGAIANWGLEIEKNAIKVDNALDY
                     QTNVEGIYAIGDVNIYPGKLKLILCGFHEATLMCQSVYQRINPGKKYVLKYTTVSGVD
                     GFDGTRKEAEKAIVKSIE"
     gene            12501..12710
                     /locus_tag="P5335_00660"
     CDS             12501..12710
                     /locus_tag="P5335_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73421.1"
                     /translation="MGDFKMDRTIVSKSSFKEANDHVTFFKDKLPVDRLNYACDIINS
                     IFNSDPLQKVDRTIISTRKHANKSV"
     gene            12691..13149
                     /locus_tag="P5335_00665"
     CDS             12691..13149
                     /locus_tag="P5335_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73422.1"
                     /translation="MPTNLFNADFLDFLELLDKHDVDFLLVGGYAVILHGYVRSTGDL
                     DLWINKTSKNYSKLKQAYLDFGTPIFSKEEFESDKFDVWSMGNEPRKIEILTHVDGLF
                     FEESKKNCNWFELEKIKIPYIDFDDLIKNKKASGRYKDLADIEELKKKKE"
     gene            13149..13481
                     /locus_tag="P5335_00670"
     CDS             13149..13481
                     /locus_tag="P5335_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRZ73423.1"
                     /translation="MQQDITIKIIDRNGIKHEVQAPTDMNMNVMELVRMYELAEEGTV
                     GVCGGMAMCASCQCYVLNDVPLPEKSPDEEAMLWEAYHVKENSRLGCQIQIKPEIDGL
                     EVQLAPEQ"
     gene            complement(13487..14206)
                     /locus_tag="P5335_00675"
     CDS             complement(13487..14206)
                     /locus_tag="P5335_00675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRZ73424.1"
                     /translation="MKRVIKLLLFSIFVFIGCSNDDDKNNFYTSTNPKPKNIKYLALG
                     DSYTIGESVCQSCRFPEQLKDSLINYFSASDNVAVKVIAQTGWTTSNLKTAISNENLS
                     PEYDLVTLLIGVNNQYQNKPFSLYETEFPELLQTAIVLAKGNLNRVVVVSIPDYAYTP
                     FGQSSSNPGNISNQLDQYNLFAKNHAESLGISFVNITDITREGLINTALVAQDNLHPS
                     ELAYSKFVERILPLAKKKLGI"
     gene            complement(14289..15467)
                     /locus_tag="P5335_00680"
     CDS             complement(14289..15467)
                     /locus_tag="P5335_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRZ73425.1"
                     /translation="MKPDLFQAPDYYLLDDLLTDEHKLVRDSARAWVKREVSPIIEDY
                     AQRAEFPTQIIKGLGEIGGFGPYIPEEYGGAGLDQISYGLIMQEIERGDSGVRSTSSV
                     QSSLVMYPIWKYGNEEQRMKYLPKLATGEMMGCFGLTEPDHGSNPGGMTTNFKDKGDH
                     YLLNGAKMWISNAPFADIAVVWAKNEEGRIHGLIVERGMEGFTTPETHNKWSLRASAT
                     GELIFDNVKVPKENLLPNKSGLGAPLGCLDSARYGIAWGAIGAAMDCYDTALRYAKER
                     IQFDKPIGATQLQQKKLAEMITEITKAQLLTWRLGVLRNEGRATTAQISMAKRNNVDM
                     AIHIAREARQILGGMGITGEYSIMRHMMNLESVITYEGTHDIHLLITGADITGMQAFK
                     "
     gene            complement(15560..16273)
                     /locus_tag="P5335_00685"
     CDS             complement(15560..16273)
                     /locus_tag="P5335_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRZ73426.1"
                     /translation="MSIFNFKMFAIQQDRCAMKVGTDSVLLGAWCPIENNPFSVLDIG
                     AGTGILSLMIAQRSNAEQIDALEIDEDAYEQCVENFENSPWRDRLFCFHAGLDEFVEE
                     PEDEYDLILSNPPFYAENYKSENEQRDLARFQDAMPFEDLIEAVNLLLSENGVFAVII
                     PHKEEERFIDLCAQAELFPIKVTRVKGSHNTPIVRSLLAFKRFELPVLSADELVIEIN
                     RHEYTNEYIALTKDFYLKM"
     gene            complement(16379..16903)
                     /gene="rimM"
                     /locus_tag="P5335_00690"
     CDS             complement(16379..16903)
                     /gene="rimM"
                     /locus_tag="P5335_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466225.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRZ73427.1"
                     /translation="MRKEDCFYLGKIAKKFSFKGEVLVYLDTDEPELYENMESVFVEF
                     NKNLVPFFIENSSLHKNDFLRCKFEDVNSEADADEIMGCELYLPLSFLPKLEGNQFYY
                     HEVIGFEVEDQRLGVVGKIVSINDTTAQPLFEVLNGNVELLIPMIDHFLVKIDRENKK
                     VVMNLPEGLVEMYL"
     gene            complement(16916..17512)
                     /locus_tag="P5335_00695"
     CDS             complement(16916..17512)
                     /locus_tag="P5335_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRZ73428.1"
                     /translation="MPVKIRLQRHGKKGKPFFWIVAADSRAKRDGKFLDKIGTYNPNT
                     NPATIDLNLDSAVQWLHNGAQPTDTARAILSYKGALLKHHLDGGVRKGALTQEQADAK
                     LAAWIDEKANKVTSKKEGLSKAEAKAKAEALKAEKVANEKRANAAAEAAKAAEVTEEV
                     AEETVEEVSEVAAEVATEETPAVEESTAAEEANEEKEA"
     gene            17722..18204
                     /locus_tag="P5335_00700"
     CDS             17722..18204
                     /locus_tag="P5335_00700"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HRZ73429.1"
                     /translation="MKKIISVVILALFFISCEEDVVLNSPSIQGKLDNVFWRAIDSEA
                     LLDENGGLTITGFGRKQKMILHTSSKNKGIYVLGNSTARYANFESDIDGTLLIYHTGK
                     DIGGDGQIEITEFDSNKGTVSGTFRFNAINDENNPLGGEILNFNQGVFYKIPVIGFTD
                     "
     gene            18352..18726
                     /locus_tag="P5335_00705"
     CDS             18352..18726
                     /locus_tag="P5335_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRZ73430.1"
                     /translation="MNIFVGSLPFSIEEADLRESFEAYGAVDSVKVVTDKFTGRSKGF
                     GFVEMPNDDEALKAIEELNGATVQGRAIVVNKSEPKPEGERRSFNNNRSGGGGGYGGG
                     GNSRGGGGYSGGGNSRGGGGRY"
     gene            18886..23400
                     /gene="dnaE"
                     /locus_tag="P5335_00710"
     CDS             18886..23400
                     /gene="dnaE"
                     /locus_tag="P5335_00710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRZ73431.1"
                     /translation="MYLIFDTETTGLPKRWDAPVTETDNWPRCIQIAWQLHDEMGNLV
                     EHQDYLVQPEGFNIPYDAERIHGISTELAQQGGISLNEVLEKFNIALSKAKFIVGQNV
                     GFDVNIMGCEFYRMNMDSPMSSMPVLDTCTEVTASLLRLPGGRGGKFKLPTLTELHQY
                     LFGVPFSEAHNATADVEATTRCFLELIKREIFTKEELDVPIDYFKNFNEQNPKEIQLI
                     GLKHINLKEASEAIRKQFQKEQPKEVAKTDLVENQQALVEVDFVHLHNHSQFSVLQST
                     ISVADLVKAAIKNKMPAVAMTDIGNMMGAFHFVRDVLYHNKSAEAKNKAAIEAGENPT
                     ETIIKPIVGCEFFVCENHKDKTRKDNGYQVVLLAKNKNGYHNLAKMSSIGFTEGFYYV
                     PRIDKEVIQKYKEDIIVLSGSLAGEVSNKLLNIGENQAEEALLWWKTEFGDDFYIELM
                     RHNQEDENRVNTSLISLAKKHDVKTVATNNVFYVDKENANAHDILLCVRDGEKQATPI
                     GRGRGYRYGLPNQEYYFKSGDEMKKLFADLPEAILNINEVVDKIEIFDLARGVLLPKF
                     DIPQDFLVAEDEQDAGKRGENLYLRHLTFEGAKKRYGTITPEIEERLDFELKTIENTG
                     YPGYFLIVQDFIRAAREMDVSVGPGRGSAAGSVVAYCLWITNIDPLEYDLLFERFLNP
                     DRVSMPDIDIDFDDEGRGRVMQYVIDKYGSNQVAQIITYGKMATKSSLRDTARVLDLP
                     LFEADKLAKLIPGMMPGKWSLARFLSDSIEEVKKAVRSDEFDNIKELIAIANEGELAG
                     ETIQQAKVLEGSLRNTGIHACGVIITPSDITNFVPVATAKDSELYVTQFDNSVVESAG
                     LLKMDFLGLKTLTLIKDTVKLVKYRTGIELDPDAFPIDDVKTYELFQRGETVGIFQYE
                     SVGMQKYLRDLKPTVFGDLIAMNALYRPGPLEYIPSFVLRKNGQEEIKYDLDACAEYL
                     SETYGITVYQEQVMLLSQSLADFSKGDADVLRKAMGKKDRATLDKMKPKFIEQAASKG
                     HDATVLEKIWKDWEAFASYAFNKSHSTCYAWIGYQTAFLKAHYPAEYMAAVLSNNMND
                     IKQVSFFMEECKRMGLRVLSPDVNESFYKFTVNDNGAIRFGMGAIKGVGAGAVETIVQ
                     NRLKEGKYKSIFDLAKRIDLRAANKKAFENLALAGGFDGFTTTHRAQYFHHDGDNITF
                     LEKAIRYGAKFQENENSAQVSLFGEASDVQIAEPVVPPCVEWNTMEKLAKEKEVVGIY
                     ISGHPLDDFRFEMKYFCNSKLDHLKHLEQHLNKTLSFGGIITSVQHRVAQNGKGWASF
                     ILEGYDESHEFRIYGEEYLKFRHFIIQNNFTFIKVMVREGWANKETGKKGDARIQFQL
                     IQYLQDVLPQFAKKLTIQMNIMMIHQNLIAELNDIFKTYKGDNTVTFEIMELEKVKNE
                     IEIKPVLIPVDEDLDMENLEDIEIEMEAPKENVKVVNQLVMPSRKLKVQISNELLSEL
                     EKKQIDFRLN"
     gene            23526..23843
                     /gene="trxA"
                     /locus_tag="P5335_00715"
     CDS             23526..23843
                     /gene="trxA"
                     /locus_tag="P5335_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRZ73432.1"
                     /translation="MALAITDATFDEVVLKSDKPVVVDFWAAWCGPCRMVGPVIDEIH
                     GEYEGKAVVGKVDVDANQEFAAKYGVRNIPTVLIFQNGEVVGRQVGVAPKKTYTDAID
                     ALL"
     gene            23955..24881
                     /locus_tag="P5335_00720"
     CDS             23955..24881
                     /locus_tag="P5335_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRZ73433.1"
                     /translation="MKIEDQKEIISGFKHLELLANQVVEGFISGMHKSPFHGFSAEFA
                     EHKVYNPGESTKYIDWKLFAKTDRLYTKRYEEETNLRCHLIIDNSSSMHYPKLKEGEP
                     FYESKIGFSVLASAVLMNLLKKQRDAVGLSVFSDAYEYYAPEKGSDRHHRMILNKLED
                     LLENPKVPKPTETVTFLHQIAEKLHRRSMIVLFTDMFQTGEETALFNALQHLKHDKHK
                     VVLFHVIDEKRELNFDFDNAPRKFIDVETGESVNLFAENIKETYEKNVESYFKKVAMT
                     CAQNKIKYVPVNVGANFEKILTTYLVEKQMFG"
     gene            24968..25041
                     /locus_tag="P5335_00725"
     tRNA            24968..25041
                     /locus_tag="P5335_00725"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25002..25004,aa:Asp,seq:gtc)
     gene            25284..25853
                     /locus_tag="P5335_00730"
     CDS             25284..25853
                     /locus_tag="P5335_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRZ73434.1"
                     /translation="MKNHRHFLIYKPYGYLSQFIYELKRTKKLLGELHDFPLGTMAIG
                     RLDEDSEGLLILTTDGMMSEVVRSKKVEKEYYAQVDGIITKEAVEQLQTGVLIGFKGT
                     KYKTKPCQAKLIADPNFPLRSQKIRDERHGPTSWVSITLNEGKFRQVRKMTSAVGFPT
                     LRLIRVRIGNFHLDKLQSGEVKELDSLLE"
     gene            complement(25870..26346)
                     /locus_tag="P5335_00735"
     CDS             complement(25870..26346)
                     /locus_tag="P5335_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRZ73435.1"
                     /translation="MSKSVKIAIAVAVCLAVGYGSGFATQSSITTWFPTLNKPFFNPP
                     NWLFAPVWTILYVMMGVSAGLIWSKMETVPILVKKALWVFTIQLILNALWSFLFFGMC
                     NPFLALLEILLLWLMIVETIKVFKSIDSLASKLLIPYFLWVSFAMILNGGIWWLNR"
     gene            26434..27522
                     /locus_tag="P5335_00740"
     CDS             26434..27522
                     /locus_tag="P5335_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HRZ73436.1"
                     /translation="MLSPSDFIPQNYTQTVEKGSFQWSAPSNIALVKYWGKMKNQIPA
                     NPSISFTLKNCKTITKLTFSKIERNPNFSFDLLFEGKAKETFRPKIQNFLERVEVYLP
                     FLKDYHFTIDTQNTFPHSSGIASSASGMAALAVNFMSLEKQLNPLMTAEYFNRKASFL
                     ARLGSGSACRSVKGKVVIWGNHEETENSSDLFGIEFVELHSNFENYQDTILLVDKGEK
                     QVSSTVGHDLMNNHPFAEKRFEQAHQNLSKIKNILKNGDVNEFVKLVESEALTLHAMM
                     MTSMPYFILIKPNTLEIINKIWKFRELTSIPVCFTLDAGANVHVLYPENVREEILQFI
                     KNELVVHCQNEQYICDEIGSGTIQLNIF"
     gene            27556..28494
                     /locus_tag="P5335_00745"
     CDS             27556..28494
                     /locus_tag="P5335_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HRZ73437.1"
                     /translation="MKGPLFYSKILLFGEYGIIKDSKGLSIPYNFYNGALKVSENPSN
                     EAIKSNESLRKFALHLEQLQAEQSELVTFNLALLNSDIERGMYFDSSIPQGYGVGSSG
                     ALVAAIYDKYANNKITVLENLTREKLLQLKAIFSQMESFFHGKSSGLDPLNSYLSLPI
                     LINSKDNIEPTGIPSQTTNGKGAVFLLDSGIIGETAPMVNIFMENLKDQGFRKMLKNQ
                     FVKHTDACVENFLQGDMKSLFSNTKKLSKVVLNNFKPMIPEQFHNIWQNGIDTNDYYL
                     KLCGSGGGGYILGFTQDLEKAKKSLKDYKLEVVYQF"
     gene            28579..29499
                     /locus_tag="P5335_00750"
     CDS             28579..29499
                     /locus_tag="P5335_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HRZ73438.1"
                     /translation="MKIISLFSVVRGYNIPVIILAQYLAAIFILSTDLRALDVLLDFN
                     LFVIVFASAITIASGYIINSFYDSKKDLINKPNKTMLDRLVSQKTKLQVYFGLNFLVF
                     LMAMLVSWRAALFFSSYIFLIWFYSHKIKKYPIVGNIMAALLAVLPFFGILLYYFKID
                     GLLENVRNEKYYVIFAHASFLFLLILIREMIKDLENIKGDMAQNYRTLPVILGESFSK
                     KVITFLIICSIFPVYILIEIYDVGYMDIYFYVSFIILIFFIMKLWNSNSKENYLLLHN
                     IIKFIIVAGVFSIILIKPSVLIHGREVLAI"
     gene            29582..30457
                     /locus_tag="P5335_00755"
     CDS             29582..30457
                     /locus_tag="P5335_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRZ73439.1"
                     /translation="MNKKEGSNRKSTSNRNTKGKPTGFSKPKPAMAKRSQKPKKVTTD
                     TPPPAAKPKVKSDDIRLNKYIANSGACSRRDADIYIQSGNVKVNGEVIIEMGYKVKPG
                     DVVNFDGATIVPEKKEYILLNKPKNFTTSVEDDRDMRNVFELIKNASQAKLQAIGRMD
                     KNTTGLLLFTNDTDMIRKFGLPNQKSPKIYQVSLDKNLKFEDLEKIAGGISFDEHRLY
                     VDEISYIDDQPKTEIGIKLRTPNVKVVRAIFEKFGYDVIKVDRVSFAGLTKKNLPRGN
                     WRALTEQEIINLKNF"
     gene            30459..30821
                     /locus_tag="P5335_00760"
     CDS             30459..30821
                     /locus_tag="P5335_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRZ73440.1"
                     /translation="MNSQEIMSIAYKWFEAFNNHNLEQLLSLYDDNAQHFSPKLKIRK
                     PETNGLVIGKEAMRDWWKDSFDRLPSLNYKVTSLTANSDRVFMEYVRSVDNEEDMLVA
                     EVLEIRDKKIVLSRVYHG"
     gene            complement(30884..33013)
                     /locus_tag="P5335_00765"
     CDS             complement(30884..33013)
                     /locus_tag="P5335_00765"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRZ73441.1"
                     /translation="MKYTFYLIILTMGLSNFQVEAQELKPIYASNPFLSSYKTPFNVP
                     PFHLIKNEHYKPAIQEGILVQRREVDGIANNKNIPTFDNTILALENSGELLTKVTTVF
                     YNINSANTNDEIQKIANEIAPLISTHSDNINLNEKLFAKVKTIWNQKDQLNLTLEQNK
                     LLEKTYKEFVRNGANLNVSDKEKLRAINNEIAVLTLKFDQNILAETNNYEYIVNKKEQ
                     LAGLPEELITAAAETAKGKGKKGKWVITLSNSSVMPFLQYCSNRQLREEIWKAYKNRC
                     NNGNEFDNNAIALQIANRRAEKARLLGYRTHAHYVLEESMAKTPEKVMTFLNQLWTPA
                     LEKAKVEEAQIKQMMMAEGIKDNVQPYDWRYYSEKIRIEKFDLDEQELRPYFSLEAVT
                     NGVFMVCDKLYGLQFKELKNIPTYHDEVTAWEVMEKNGKHLGILYMDFYPRASKQGGA
                     WMTSYKPQQKEGDKRIPPIISIVCNFSKPTADAPALFTFDEVTTYFHEFGHALHGLLS
                     NVSYKSLAGTNVYRDFVELPSQVMENWAADPAVLKMYAKHYKTGEVIPDALIQKLENS
                     GTYGQGFATVEYLASAFLDMDFHTSTTTITGTAEAFENESMKKIGLIPSIIPRHRANY
                     FSHIFAGGYSAGYYSYIWSGVLDTDAFYQFKKTSLFDSEKADSFRINILEKGGTQEPM
                     ELYKKFRGSEPSIEPLLKKRGLETQSK"
     gene            33237..33890
                     /locus_tag="P5335_00770"
     CDS             33237..33890
                     /locus_tag="P5335_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73442.1"
                     /translation="MKMVKVLFVFFFSISFLVSCADDIDDNIVSAPSDFYYLGELPIP
                     FYTNGNTDFPNIDWGNESGVFTLNENYQGVSINPSNGVLSWNEDLPLGENIISVTATN
                     SAGFAVTSVLFLHQFGGEFTGGYNSDPTSTTVSASNLVINFNVNGTLVITDNGETVNG
                     AWSFNPQGKLVCNYMLSSGSYQLIYDLTYSVTVNPFLEGYKRLEGSTVNLGFSRMDY"
     gene            complement(34000..34081)
                     /locus_tag="P5335_00775"
     tRNA            complement(34000..34081)
                     /locus_tag="P5335_00775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34045..34047),aa:Leu,seq:tag)
     gene            34218..35606
                     /locus_tag="P5335_00780"
     CDS             34218..35606
                     /locus_tag="P5335_00780"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRZ73443.1"
                     /translation="MDNIKNYVQQNKERFISELIDLLKIPSVSADSAYSQDVIDTAHA
                     VKASLEKAGCDFVELCDTPGYPIVYGEKTIDKSLPTVLVYGHYDVQPPDPMDLWTSPP
                     FEPVIKTTEIHPEGAIFARGSCDDKGQMYMHVKAFEYMVANNCLPCNVKFMIEGEEEV
                     GSKSLGWFVERNQEKLANDVILISDTGMISNQQPSITTGLRGLSYVEVEVTGPNRDLH
                     SGLYGGAVANPINILAKMIASLHDENNHITIPGFYEKVEELTTAERAEMAKAPFSLDN
                     YKKALDIADIYGEAGYVTNERNSIRPTLDVNGIWGGYTGEGAKTVIASKAYAKISMRL
                     VPNQDWEEITELFKNHFVSIAPKSVKVKVTPHHGGQGYVTPIDSIGYKAANKAYTETF
                     GVPAIPVRSGGSIPIVALFEKELKSKTILMGFGLDSDAIHSPNEHFGIFNYLKGIETI
                     PLFYKYFVELSK"
     assembly_gap    35677..35776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            35825..36925
                     /locus_tag="P5335_00785"
     CDS             35825..36925
                     /locus_tag="P5335_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HRZ73444.1"
                     /translation="MLKKFFLLCSGSDKQIIESCSDGEQNKYAGIGATVFFTAVMAFI
                     AGSYALYTVFDNIYTALFFGLVWGLLIFNLDRFIVSTIKKRDSFLDEFIQASPRIVLA
                     VIIAIVISKPLEIKIFEKEINTVLLKEKNEMALNNKKQVANFFQTDVDQNKAQIDSLK
                     SDILKKEKEVNDLYAVYITEAEGTAGTKKLGKGPVYKEKREKHDAALQELATLKTTNQ
                     TKIIELEAKGKTLQTDLDKKVADTQPIIDGFDGLMARINALDKLPWLPSFFIMLLFLA
                     IETSPIIAKLLSPRGEYDFKQEDGELAVKNVLAQNKYQSDLQKQTDASIYDKVYADIR
                     EDKGLYDYKKKRAIELLELQADGFVEKQRKSL"
     gene            complement(36985..37488)
                     /locus_tag="P5335_00790"
     CDS             complement(36985..37488)
                     /locus_tag="P5335_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HRZ73445.1"
                     /translation="METKEKKPLEKVCIICAKGNLEDVYASLVMANGAVMEGIETNLF
                     FTFFGLEGITKKSMNHLHTATTGNPAMKMPGGLPFPTMLGGLPGVESAVSSMMRKQMD
                     ELGMPPVDEFLEMITAGGGTIYACKLAVDMFKLTKEDLSDEVTDIITIGEFYELCDGD
                     RTQIIFT"
     gene            complement(37504..37815)
                     /locus_tag="P5335_00795"
     CDS             complement(37504..37815)
                     /locus_tag="P5335_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HRZ73446.1"
                     /translation="MEKVIAGKSVSVDSNGYLTDLSQWNKEIAIEIGKEEGIEEVTPK
                     HFEVLAYLQDQHKKDIALTIRGVGKSGIVDIKELYALFPKGPLKISSKMAGIPRPKSC
                     I"
     gene            complement(37846..39081)
                     /locus_tag="P5335_00800"
     CDS             complement(37846..39081)
                     /locus_tag="P5335_00800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRZ73447.1"
                     /translation="MKNLVILGAGTAGTMMASHLIKKLNKSEWKITIVDQYKTHYYQP
                     GFLFLPFDTYKPEDVKKTIDEFIPKGVKLVREKIESIDKDSDTVLLDTGEKLKYDILI
                     IATGTKTAPGEVEGMLGDYWYKTIFDFYTFEGALALRDKLKTWKGGKLVVHITEMPIK
                     CPVAPLEFAFLADSFFLNKGMRDKVEITYVTPLSGAFTKEKCTTALTYLLKDKNIKIV
                     PDFAVEHIDGENGKIVDYGGDEVPFDLLVTIPTNMGDEVMARSGFGDDMNYVPTDKHT
                     LQTTVKENIFAIGDCTNVPASKAGSVAHFQAEILTENILLYIDGKELKPDFDGHANCF
                     IETGHGKALLIDFNYEYEPVKGKFPFAGIGPLNLLTESRLNHWGKLAFRWIYWNMLLP
                     ARKLPFITPKMSKAGKNFN"
     gene            complement(39315..40637)
                     /locus_tag="P5335_00805"
     CDS             complement(39315..40637)
                     /locus_tag="P5335_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRZ73448.1"
                     /translation="MKKILFPVCLFLLSSFAYSQSGHIMQGVGAVNMSMGGASTAQPL
                     DISGALQWNPASISAFNDKIIKFDIGMFYSSPELFSTVPEFNELGQPTGNFFSGSTKD
                     DRGVSPMPALAMVWGKEGSKHTFGASAFGISGFGVTFPESTTNPINMPQSMGGFGRVE
                     SDYMLLQVGFTWAYELSEKFSIGIEPTFNYATLELMPNPTANPSQAGYPSTDKANATG
                     FGAQIGLFYNSGKGFKAGASYKTSQYFSDFEFKNTYLDNSTGTNKFNMDYPAILSFGL
                     GYSKSDFDFALDYRMVDYENTDGFSETGWTPTASVAGFGWDNISIVSAGIQYKGVNKF
                     PLRLGYTYSSNPISSDVAMFNIPATAVIKNAYQLGFSYLAGENISLDAVYHHGTSSGK
                     TSGQLLNPMFASSSNPYGAIPGSNVSYDMTTDLIMVGISYKFSKKQTN"
     gene            complement(40949..>41183)
                     /locus_tag="P5335_00810"
     CDS             complement(40949..>41183)
                     /locus_tag="P5335_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRZ73449.1"
                     /translation="GTPSLNLKCDPEKALELRAEYDAINPGYHMSKIHWNTVAFHSDV
                     DDKMMRELINHSYELVFKSLSKKLQNEIVLQNK"
     assembly_gap    41184..41283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<41284..41918)
                     /locus_tag="P5335_00815"
     CDS             complement(<41284..41918)
                     /locus_tag="P5335_00815"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRZ73450.1"
                     /translation="MIATITHQNKTFQVDLSKPIDISLPLSNTEKNPIAWYIEKPQIE
                     PVRFGSWVGSVKGGATTNFNNIFFNPHGHGTHTECLGHITKEFYSINQALKTFFFTAQ
                     LISIQPEKQGEDFVITKMQVEKALGNSKPEAIVIRTLPNLNEKKSKNYSHTNPPYLHE
                     EAAIFIREKGIQHLLIDLPSVDKEKDEGKLLAHKAFWNVKDVNNLNEDARK"
     gene            complement(42061..>43152)
                     /gene="hemW"
                     /locus_tag="P5335_00820"
     CDS             complement(42061..>43152)
                     /gene="hemW"
                     /locus_tag="P5335_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRZ73451.1"
                     /translation="CHYCDFHFSTSMKKKDEMVLALVKEISMRKEEFKNDVIETIYFG
                     GGTPSVLAIEDLRLLIEEVFKNYNVIENPEITVEANPDDLSNNRIIELSNNRINRLSI
                     GIQSFFEDDLRMMNRAHNVEEAKICLEEATKHFDNISLDLIYGIPGMSNEKWIQNIET
                     ALSFGIPHISSYALTVEPKTALHSFIQKGIIPPPEDEVAQEHFEILVEKLEEAGFIHY
                     ELSNFGKENFFSKNNSSYWLGKKYIGIGPSAHSYDGEKRGWNVSNNSLYIKSIQEGKL
                     PIETEILSKTDRYNEYVMTGLRTIWGISLDRIQSEFGQTYLDYLNQQAAKYIEDHLLH
                     VDENILRTTRKGKFLSDGISSDLFMLNLI"
BASE COUNT        14145 a         6974 c         7583 g        14250 t
ORIGIN      
        1 atttcaatga tttggtataa caaagataat aaaatatttc aattaccata ccaaattttt
       61 tttgacataa aaatattgct aaaacgagaa caattattca atagttgtta tgcctattga
      121 aacccatttt cttttattat tatcataata ggtattagag tctgggaatt caatgtaaaa
      181 ttcacctttt gcattttcgg tgtaataggg tgttaaatct tcacctgcaa tatacttttc
      241 aaaaaggaat ttactttttg tttttaactc agtaatctcc attttccatg ttctgaatgc
      301 ttcttcatag gaaattattt ttcctttagc atcttttttt aatttaccta tggtaactac
      361 tttttttact tttaaactag gagcaattcg tgcaatggca aaatatattt catttgattt
      421 tttatcaaca aaataatgat ataattctgc atttttagct tttacttgat agattgaatc
      481 aaattgacta tcaaattttg tttcgtgcgt ggcctttttt gctaatttgt ctgtgtaacg
      541 tataatttca tatttgaacg actcgacctg ttcttcattc aaataatcgg aagccaaaat
      601 tttttgaggt tctttttgac aagaataaaa aagcacaaga ataaaaagcg agtaaataaa
      661 tttcattttc aattttattt tttataaaaa gagcctaatc taaatagatt aagctctttc
      721 aaatataata attttaagac tagtatactt tagttaccgg gttaaattca gcccaaccat
      781 cagtccaatc tgttgatcca aatgctcctc tataagtagt tggagtaaag aatgcatcgg
      841 ctaatttagg atttgtaaaa gctgcacctg aagttaacgt acctgaagta actctaaaat
      901 ccgggtttga agcataacct gtacttgtgt ttgttccgaa gaatatatta gtatttaaac
      961 ctaattctaa atagacagca ggtaaatctg tatttgtgat tgtttcgtta gtagcattaa
     1021 accaagtttg aacatctgaa gctgttgaag tcatccctgt tccaacagag aatgttgttg
     1081 ttggagctac cataacgtta tttgttacta cacctaaacc agaactgtaa ttgtcataaa
     1141 ctgattgttg attcatacgt aaacctcttg ggaaaccaac aaatactgaa ttagcaattg
     1201 ataaagctgt tcttcttctt aaatcaagcg aatgttggta gttaccattg attgattgtg
     1261 tgtttgaaat tcttggacca acaccagtaa tgtttgagaa aacaccggtt gtgattaacg
     1321 ttgttaagtt gtcatttgga ccattatctg tttcaaaagt gtttgaacct gattgatcgg
     1381 cataactacc ataacgaaca gaaactccaa attgaacttt tcctgtgaaa ccgtaatcaa
     1441 catcaaaaca gtcatcccac attgcaaatg taaccaagtg ttttgaacta acagaaccac
     1501 caaaccactc aaaaccgtca tcacctccga aagaaacttg acagtagtcc atttgagttc
     1561 cactacctac tccacccatg gtgatagagt ttgtctcgtt gtttggggtt aattcgatac
     1621 ctgcaaattc cacacgtaca tattttaaaa tacctgaatt gtctgaatcg tttgttcctc
     1681 caaaaattac tgcaggtgta ataccttcaa ttgccgggtc aggttgatta accggtgcat
     1741 ttcctaatat cactattcca ccccaatcac ctctgtcgcg agttccggct gcaagagctg
     1801 aagtgaatac aattggttct actgaagtac cttctgcgat aagtttacca cctttgtcaa
     1861 ttaccaaagt accttttgtt gctttgtctc caaaaattat agtgcccggc tcaattgtta
     1921 aaacaacacc tgttctaacg aatacttgtc ctttgattaa atatttttta tcttttgtaa
     1981 gcgtacgagt ggctaaatct cccaccaatt cgactggtaa atttggttct tctacaggag
     2041 gtgttacatc tacaacaatt ggattgtcgt cattgctgca agatgtcatc gaaatagcaa
     2101 gaactgctat gaacatacta gcttttaaaa ttagattttt tttcataatt ggttgttttt
     2161 aaataattta tagttgttta aaagttatgc gataagttta gagagaaaag tgtacctttt
     2221 ttgaatacag aggtagcatt atcaatgtct ttactaattt tttcatctct gtttgagtct
     2281 tcgtaaaatt gatatttagc atttaataga tcttgaattc ccaattttat tgttgttttt
     2341 ttgaaagttt tagaaaatgt taagtcaaga ctgtttctgg aaagttcata tatagttgga
     2401 aagttgatat ctccaaccga aaagattctg tctccaaatc ggttgtaaat tacattcgca
     2461 gaaaaaccgg tttcatcagt ataacctaga gcgacattga cgatgtatgg cgattgccct
     2521 tgtaaggcac gcacttgtgc ttgtgatgta acatctcctc ctaaatctac ttcagaaaag
     2581 atatatgaag cattaatatt ggctgaaagt cttcctaaat aaaggttgtt ggttaattct
     2641 ttgaatgatt ttctgatttc aagttcaaca ccataattgt aagcactgtc tgcattggca
     2701 taattaaact ggggttgttc tgttgtgatt tgcgtaacat tttcaattgg attatcaaat
     2761 cttttgtaaa atgctcccaa actcatagtt tctcctttgt ttgggtaaaa ttcgtagcgt
     2821 aaatctatat tatcaatttc agcagttttt aactcagcat taccaacttt cgccgcttca
     2881 ttttcataat cataaaacaa gaaaggggca atttctctaa attctggacg gttaacggtt
     2941 ctactataag caaatcttac taaagctctt tcgctgatat tgtatccaac atttaatgaa
     3001 ggtaaaattg aagttaccgg attttcaatg tcaattggac ctaaatcagt gttactttga
     3061 agtgatagta tgttgtgctc tactcgaaca ccagctgaaa tatcaaattt atttaaagga
     3121 aattcaccat aaatataccc agcggctaag aaattatcag catcataaga gtcaattgaa
     3181 cgggttcctt cttttaaaat ccaaccatcg gttgtgttta cattttcagg actaaaaatg
     3241 tcagttagag gtaattgttt taactctgtg attcgatcaa tcccataatc cggaaggaaa
     3301 tatgaaacat atcttgaatc aaaagctctc tttctgtaat ctaaatagta acctgttttt
     3361 aatttgattg ggtttaattc ttcatcattt tttatgcgtt cgatgttgtg cgtataattt
     3421 gcaccattgt ttacggagta ctcacttaat gtaccgaagt atctaccggt atcaaacaag
     3481 ttggttgaag ccggatcaat catttcgtaa accgaatttg gatcttcaac atctttaata
     3541 gttctgaatc ttcttaaatc cggttcggat tgatacatgt aattataacc ggccacccaa
     3601 tcaattgaat ttgtactatt taattggtga ataccttcta attgtccggt ataaatagaa
     3661 cgtgacgtgt aacctaataa gtaatttcta aacaaatcct cacctctttg taaatagttg
     3721 ttaccatttc tgatgattgt ttcattttca ccaatttggt taaataaatt tttaaacttg
     3781 atggtgttgt cgttgtctag tttaaaaatc cagtttgata atacggttac ccgagcttca
     3841 ttttgattat aatcatctaa ataatttagc cattgaggag gtcttgtttc accttcattt
     3901 aacgtggtgt atctcgtgaa ctgtctttgg taagttaggt aagtatttga atagccaaac
     3961 atatttaccg attgtaagtt cattccgtta gcaaattttt tacttcttcc gaaagcaaaa
     4021 ccgaaattat tgtctaataa agtagtgctt ttatttgggt tgaaattatt ttgtaatttg
     4081 gtatttgaag cagtaactcc aacttcagga taagtgttaa ttaaatttgc cgatggaaaa
     4141 gtgctaggta gcgttctaaa ttcatagtca aaacctaaaa agtcagtttt agatccctca
     4201 ctctgataat atgtatcaaa agaagtatcc attctgtatc caaatccaac attgaaagta
     4261 ttgaattctg ttatgttttc tgaagttgtg atattcacta tacctcctga aaaatcaccc
     4321 ggtttatctg ctgagccggt tttaatgata accattttat ccattgcttt agttggaatc
     4381 aaatcaaaag caaatgttct tttgtcaact tctgtacttg gagcaacggc attatttatt
     4441 aatacattat tataacgttc gctcaatcct cttatcatta caaatttgcc atcaacaata
     4501 gtaactcccg gcactctttg aatagcatct gcagcattac tgtcggttcc ttttgacatt
     4561 tgttcggctg aaatagcact aacaacttgt ttggcttctt taatttctat catcacagca
     4621 gcttctgtgt tttttcttcg agaggtaacc accacaacat cttgtagcat tacaccttca
     4681 cccgaggtca aagccttgtt aacagttacc gtttgtcctg ctttaactgt tattggcact
     4741 tctacagttt gataacctaa aaagcttagt tgtagaatat aatttcccgg ttcaacagat
     4801 aaagaataga accctttttc atctgtggta gtccctatac ttgtgccttt aattaatgca
     4861 tttgcaaaag gtaaaggttc attattcata tctttgtcgg taagtacacc ggaaacagta
     4921 cctttttggg cagttgaaaa gaaggtgata aatagggtaa taataagtaa tctaattttc
     4981 attattttgt ttcattaaat ttgaggcaaa gaaacggaca ccttgtaaag ttggagttac
     5041 tcacttatta tgattgtgtt tcattcatgt tatgaaaagg ttaccacatt aaattaccgt
     5101 taagaacgtt tttcattttg tattatcttt atctttacat cgctaaagca caaataaaaa
     5161 ggaatgaaaa aaaaagacat taagatatta ttggttgacg atgaacaaga tatactcgaa
     5221 atagttgggt ataacttgtc tcaggaagga taccaaatag ttacagcaac aaatggtaaa
     5281 gaagccattc aaaaagctaa aaaagaatta cctcacctta ttataatgga cgtcatgatg
     5341 ccagaaatgg atggaatgga agcttgtgaa aacatccgaa aaattcctga attgagtaat
     5401 gttattattg cttttttaac ggctagaagt gaagattatt ctcaagttgc cggatttgaa
     5461 gccggagctg atgattatat tgctaaacca attaaaccaa aagtattggt gagcaaagta
     5521 aaagcattgc tgagacgttt aaaagaagaa gatccaaaag gggatatact aaccgttgga
     5581 aatattgaag ttaacagaga agaatataaa attatccaag acggaaaaga aatagtttta
     5641 ccaagaaaag agtttgaatt gttttatcta ttagcctcta aacccggaaa agtatttaag
     5701 cgggaagaaa ttcttgataa agtatggggc aatgaagtga ttgtaggagg aagaacaatc
     5761 gatgttcata ttcgaaagtt acgtgaaaaa attggtgacg aactgtttaa aaccatcaaa
     5821 ggagtaggat ataagtttga aatttaattc tttgtttgac tttgcattca gaatagctta
     5881 cttttgaatt tagtatgcaa aaacaaactt ttttcaatgg ctattagttt taaaaagacc
     5941 tataaattcg cagttaaatc tgctttttac attacttttt tttcaatggt ttttttaacc
     6001 ttattgttgt attacttcaa tcaggtgagt tatgttttga ttactgtatt tacaatttcc
     6061 atctatcttt tctctttttt tgttgtacaa tatcgtgtag aacgattcat ttatcgtcgg
     6121 gtaaaaaaaa tatatgatga tgtttctttg ttggattcta ccactttcag aagtcaaccc
     6181 atcacaacag atatggcaac cttgaccaaa gaagtcaaaa aattcgctac cgataaaaaa
     6241 ttagaaattg aaaccttaaa agttcgtgaa gaataccgga gagaattttt aggaaacgtt
     6301 tctcatgagc tgaaaacccc tctttttacc gttcaaggat atatttctac tttattagat
     6361 ggtgctgcaa atgataaagt tctacgaaaa aaataccttg aaagagccga aaaaggagta
     6421 gagcggttaa tttatatcgt caaagattta gatatgatta ccaaactcga aaccggtgat
     6481 ttaaatttag attattccat ttttgatatc gtagaaacaa ttaaaaatgt ttttgatttg
     6541 ttggaaatgc gtgcttccaa aaagaaaatt actttaacct ttgatactaa ttacagtaaa
     6601 ccgatttacg ttcgagccga tcaggaaaaa atccaacagg taattactaa tttagtaatg
     6661 aattcaatta agtatggaaa agaaggcggc actactgaag taagtattga agatttggta
     6721 aataaaaaaa taatcattcg tattacagac aatggcgaag gaattcaaaa acaacatatt
     6781 cctcgtttgt ttgaacgttt ttacagagta gataaaagtg gtgcccgttc tgaaggtggt
     6841 tcaggtttag gcttagcaat tgttaagcat attattgaag ggcataatga aaaaatttat
     6901 gtagaaagtc aattcggagt cggttcggag ttttcattca cgctcgaaaa gcagaaataa
     6961 ttttttccaa tctaaatcat atttgaaagt atttaatcct ctgtacaaat ctatttcaaa
     7021 taaattttca attacaaatt tttcttgttt agcatagggc acgatgcctt cttttttgta
     7081 ttttttggct aaatactcct gttcaaattg accgggagtt ggtataaaaa aggctttttt
     7141 tcctaatttt gccaaatcca ttaccgtagt gtatcctgaa cggcaaagaa caatttcact
     7201 ttcattgaaa gtttgttcca actcaacgga agtcataaaa ttataaaaag taacatgctc
     7261 tgtttgttct gatttttgtt ccggttcaat cacacctcta acaaaaacta cttttccttt
     7321 gtatcgtttt atttcttctc ttaaatgcaa ttctaatatt gaacgttgtg gttcgggccc
     7381 tgaaagtata atcagtaaat catatttttt aggcaactct ttttttgtaa atcgacttaa
     7441 cggaccaatg tagtttattt taaaatgagg gttttctaaa tgacctagtt ttcctgtcaa
     7501 atttggaatt tctgctacat caggaaccca acattcatca tattttttaa taatatactg
     7561 atgtaattta ctcgtaatcc aactcgtgtt tcctgttaat acatttaatt gatgcgtaat
     7621 gaaaacggaa ggaacttttt tggaataaat ccctaaccga ttatcagaaa tgattcccga
     7681 aattccgtat tctttaaccc attttttgac aagttttcgc tcttgataaa tggcttccat
     7741 catttttgga ctgtttttaa tcaatttcca cttaaaaaaa gcaccttttt tagcatattc
     7801 aatttgataa gaaggcagtt caagaaaagt aagttgagga aattcttttt gcaaaatgtg
     7861 caaagcaaca ccatcagaag caataattgg ttcatagcct tgttctatta aagcttcaat
     7921 aattgggatg catctggtag catgccctag gccccaattt aagggagcaa caagaatttt
     7981 tgggtgggtt gtttccaagt cttaataact catttaataa atgtaaaagt actactattt
     8041 ttgattgagt atatttccct aattttacca aaaaattaaa ttacgtggga agtaagaata
     8101 aattaaagcg tttcaaggaa aatgaatctt ttcaaaatgt tatagaacct actcgagaag
     8161 aagtagtttc cggagacttt aaatggaaag gcaattggaa taatgatttt tttaagaatg
     8221 aaaaaccaat tgttttggaa ttagggtgtg gaaaaggaga atactcagtg ggattagctg
     8281 aacgttatcc ggataaaaat tttattggaa ttgatattaa aggagcccga ttttggcgag
     8341 gtgctaaaac agctgttgaa aacggaatgc ataatgttgg gtttcttcgt actcaaattg
     8401 aattagtaga attttgtttt gctgaaaatg aagtggatga gatttggatt acttttcctg
     8461 atccacaaat caaatataaa agaacgaaac atagaatgac taatactgaa tttttgcaac
     8521 ggtataaaaa agtattaaaa aaggatggtt tagtgcattt gaaaacagat agtgaattta
     8581 tgcatggtta tacactaggg ttgcttcatg gtgaagggca tgaggtgatt tatgccaatc
     8641 ataatgtata taaaaatgaa ggagctccgg aagtggtaac ggctattcaa actttttatg
     8701 aaaaacaata tctagaaata aataaggcaa ttacctatat tcaatttagg ataaaataat
     8761 tttaacgcat attctgttca atgaatataa ccttaccaat tttactcgga ctttcctctg
     8821 cggctttagg aacaactttg cccggtttgc ttaatatgac agtagcaaaa gtgggcatgc
     8881 gagatggcaa aaacagagcg gtttggtttg cattaggagc ttcagtgatt gtattttttc
     8941 aaacattaat agctgtttta tttgcaagat ttatagacgg tagagctgat attggtgctc
     9001 ttttacaaga aataggattt gtgattttta caggcctcac tttttatttc ttttggagtg
     9061 gaaaaaaaaa taagctcaaa ataaagaaag atgaaattaa aatgcgaagt aaatccagtc
     9121 gtttttttat gggagtttta ctgtcatctc ttaatttgtt tcctattcct tattatgttt
     9181 ttataagtat tacgttggct acgtatcatt atttttattt tgacaattat tttatttatg
     9241 ctttttctac cggtacagct gttgccgctt ttttaatttt tttagggtat atttcttttt
     9301 ttaaaaaaaa ggacagaagc aattcttttt tgtcaaaaaa cattaattat gttattggta
     9361 ccatcacggg tttagttgcg ttgatttcac tttttaaact attttttaaa gattagtaaa
     9421 acaaccatta tggaggaaaa tttcttcgaa agagtctact tcatcgttcg ccaaattcct
     9481 tatggtcgtg taacttcata tggagcaatt gctaaagcgt taggaactgc ccgttcggct
     9541 cgaatggtag gttgggccat gaatgcttca catcatttag aagatgttcc tgctcataga
     9601 gtagtcaatc gaaaaggatt gctttcagga aaacatcatt ttagtgggac taatttaatg
     9661 cagcaattat tagagaacga agggattgaa gtaattgata atcagattat tgactttgaa
     9721 aaggtttttt gggaaccgga aatttccaat tagcaattat tttctaattt cttagtctat
     9781 tattcgtcgc tatttcctta tcctgacata aacattttca attctttaac tactgtttct
     9841 atacttttcg tattatcttt gcaatctata ataagtttaa ataaaggtaa ttattattcc
     9901 tttaaaaaaa atataaataa gatgaaactc gatagaaaag aaatactaaa agccctagaa
     9961 actatctctg ttgccggtga aggaaaaaat atggtcgaaa gtggagccat tcaaaatgta
    10021 ttaacttttg gcgatgaggt tattgtagat ttacttttgt ctacgccagc tttgcatatc
    10081 aaaaaaagag cagaagtaga catcatcaaa accattcacg aattggtcta cgaaaaagcg
    10141 aaagtaaaag tgaatatcaa agttgaaact cctgaaaaac cggagaatcc taacttgatc
    10201 aaaggaaaac aaattccggg aattagtaat attgttgcta ttgcctccgg aaaaggaggt
    10261 gttggaaaat ctactgttac tgctaattta gcagtttcat tggctaaaat gggttttagt
    10321 gttggtgttt tagatgccga tatttatggg ccttccatgc caattatgtt tgatgttgaa
    10381 aatgcaaaac ccttatcggt agaagtagat gggaaatcaa aaatgaagcc ggttgaaagc
    10441 tatgaagtaa aaattctatc tatcggattt tttactaaac cggatcaagc ggttatttgg
    10501 cgagggccta tggcagcaaa agcattaaat caaatgattt ttgatgctga ttggggtcaa
    10561 ttggatttta tgttaatcga tttgcctccc ggaaccggag acattcaact ttcaatcatg
    10621 caatcgttac caattacagg tgcggtagtc gttagtacac ctcaagctgt tgctttagca
    10681 gatgctaaaa aaggagtttc aatgtttatg tcagaagcaa tcaatgttcc tgttctggga
    10741 attattgaaa acatggctta ttttacgccg gaagaattac caaacaataa atattatatt
    10801 tttggtaaag aaggagctag aaatttggca gaagatttaa aagtgccttt attaggggag
    10861 attcctttag ttcaaagcat tcgtgaagca ggtgattatg gtcgacctgt cgctttacaa
    10921 acggcaacgc ctcttgaaac ggcctttgaa aatatcgcca gaaatattgt tcaggaagta
    10981 gtaaatcgaa atgaaaattt acctccaagt gaagcgatta aaataacgac aatggctggt
    11041 tgttcagctg taaataaaaa ataatgacta cagaagaaat tagattaggt attgaaaagg
    11101 ctttagatga aattcgtccg tttttgttat ccgatggggg aaatatttca ttagttgatg
    11161 ttttagacag caaacatgta agggtacgtt tggaaggtgc gtgtgtggga tgtagtgtga
    11221 atcaaatgac tttaaaggcg ggtgtggaga ctactatcaa gaaatatgtg cctcaaattg
    11281 aaacagttga aaacatcgct taaagtttga aatcagaact caagtgttat tttatgaaac
    11341 tgatataagt catgttttta aattataaac aagtttaatt tcgtcccaca aaaaactcag
    11401 aacataaaaa agaatgatac aaaccgatat ccttattatt ggagctggcc cgacagggct
    11461 ttttgccgta tttgaagcag gcttattaaa attgaaatgt catttaattg atgcacttcc
    11521 tcaaccaggt ggacaattag cagaattata tcctaaaaaa cctatctatg atattcctgg
    11581 ttatccggaa gtattagccg gtgatttggt gaccaattta atggaacaaa tcaaacagtt
    11641 tcaacctgga tttaccttag gagaaacggc tgaaaccatt gataaattag aggatggtag
    11701 ttttattgta actaccaata aaggaacaaa acatcaagca aaagcaatcg ctatcgccgg
    11761 cggattagga acatttgagc ccagaaaacc tttgcttaaa gatattgagt tttatgaaca
    11821 gaacgatcgt ggagtagatt attttgtaaa agatcctgag aaataccgtg gaaaaaaaat
    11881 tgtaatttcc ggaggtggtg attctgcatt agattggagt attttccttt ctgacgttgc
    11941 atctgaagta acattggttc accgtagaaa tgaatttcgt ggagcattag attcagttga
    12001 gaaagtacag gaattaaaaa atataggaaa aattaaatta attactcctg ctgaagtaat
    12061 cggatttaaa ggaactgaac gtattgaatc tgtagatgtt gagatcaatg gtgcccgaat
    12121 gaatgttgca acggattatt ttattccctt attcgggtta acaccaaagc ttggtgctat
    12181 tgcaaactgg ggattagaaa ttgaaaaaaa tgcgattaaa gtagacaatg ccttagatta
    12241 tcaaacaaat gttgaaggga tttatgccat tggtgacgta aacatttatc cgggaaaatt
    12301 gaaattgatt ttatgtggtt ttcatgaggc tacactgatg tgtcaaagtg tttaccaaag
    12361 aatcaatccg ggaaaaaaat atgtattgaa atacaccact gtctccggtg tagatggatt
    12421 tgacggaaca agaaaagagg ctgaaaaagc aattgtaaaa tcgattgaat aaatttttat
    12481 ctttgtttaa aagaattaaa atgggtgatt ttaaaatgga tagaacaatt gtttcaaaat
    12541 cttcttttaa agaggcaaat gatcatgtaa cattttttaa agataaattg ccggttgata
    12601 ggttaaatta tgcttgtgat attattaatt ctatttttaa ttctgatcca cttcaaaaag
    12661 tagatagaac tatcatttcc actcgaaaac atgccaacaa atctgtttaa cgccgatttt
    12721 ttagattttt tagaacttct tgataaacat gatgttgatt ttctcttagt tggaggttat
    12781 gcagttattc tacatggcta tgtcagaagt actggagatt tagacttgtg gattaacaaa
    12841 acttcaaaaa attatagtaa attaaaacaa gcatatcttg attttggtac tcctattttt
    12901 tcaaaagaag aatttgaaag cgataaattt gatgtgtgga gtatgggcaa tgaaccaaga
    12961 aaaattgaaa ttcttaccca tgttgatggt ttattttttg aagaaagtaa gaaaaactgt
    13021 aattggtttg agttagaaaa aattaaaata ccttatattg attttgacga tttaataaaa
    13081 aataaaaaag cttctggaag atataaagat ttagctgata tagaagaatt aaagaagaaa
    13141 aaggaataat gcaacaagac atcaccatca aaatcatcga ccgaaacgga atcaaacacg
    13201 aagttcaagc tcctaccgac atgaatatga atgtcatgga attagtgcga atgtatgaat
    13261 tagccgaaga aggaactgtt ggggtttgtg gcggaatggc catgtgtgct tcttgtcaat
    13321 gttatgtatt aaatgacgtt ccactacctg aaaaatcacc tgatgaggag gccatgctgt
    13381 gggaagcgta ccatgtgaaa gagaatagtc gtctaggttg tcagatacag attaaaccgg
    13441 aaattgatgg cttagaggta caattggctc cggaacaata aaaatattaa attcccagtt
    13501 ttttttttgc taagggtaaa attcgttcca caaatttaga ataagccaat tcagatggat
    13561 gtaaattatc ttgagcgact aatgctgtat taatcaatcc ttcgcgagta atatctgtaa
    13621 tattcacaaa acttattccc aaagattccg cgtgattttt agcaaaaaga ttgtattgat
    13681 ctaattgatt agaaatgttt cccgggtttg aactgctttg tccaaaagga gtataagcat
    13741 aatcaggaat cgaaacaaca accactcgat tcaaatttcc tttcgctaaa acaatagcag
    13801 tttgaagtaa ttccggaaat tcagtttcat ataaactaaa aggtttgttt tggtattggt
    13861 tattgacacc aattaataag gtaactaaat catattccgg acttaaattt tcattcgaaa
    13921 tagcggtttt taaattggag gtagtccaac cggtttgagc aataactttg acggcaacat
    13981 tatccgacgc agaaaagtaa tttattaaac tatctttcaa ttgttccgga aaacgacagg
    14041 attggcaaac actttctcca attgtataac tatctcctaa tgctaagtac ttaatgttct
    14101 tcggttttgg atttgtggaa gtataaaaat tgtttttatc atcatcattt gaacatccga
    14161 taaaaacaaa aatgctaaac aataaaagtt tgattacacg cttcattatt gttgaaatta
    14221 aattagtcaa aaatagaaaa gcttccctta gtagagaagc ttttgtgtat tttttaagga
    14281 ttgttcactt atttaaaggc ttgcattccg gtaatatctg ctccggtaat taataaatgg
    14341 atatcatgtg ttccttcata tgtgattacg gactctaaat tcatcatgtg ccgcataata
    14401 gaatattcac ctgtaattcc catgccgcca agaatttgac gtgcttcccg agcaatatga
    14461 atagccatgt caacattatt acgttttgcc atggaaatct gagccgttgt agctcttcct
    14521 tcatttctca aaactcccaa tcgccatgtt aataactgag ctttggtaat ttcagtaatc
    14581 atttcggcta atttcttttg ttgcaattgc gttgctccaa taggtttgtc aaactgaatt
    14641 ctttctttag cataacgcaa agcggtatca taacaatcca ttgcagcccc tattgctccc
    14701 caagcaattc cgtagcgagc tgagtctaaa caacctagcg gagctcctaa gcctgatttg
    14761 ttgggtaata aattttcttt aggcactttt acgttgtcaa aaatcaattc accagtagct
    14821 gatgcacgaa gtgaccattt attatgtgtt tccggagttg taaaaccttc catgcctcgt
    14881 tccacaatta atccgtggat tctgccttct tcattttttg cccaaactac ggcaatatct
    14941 gcaaaaggag cattggaaat ccacattttt gctccgttta ataaatagtg atcaccttta
    15001 tctttaaaat tagtggtcat tcctcccgga tttgaaccgt gatcgggttc agttagccca
    15061 aaacacccca tcatttctcc ggttgctaat ttgggtaaat atttcatgcg ttgttcttca
    15121 tttccgtatt tccaaatcgg atacattact aaggaagatt gtactgatga agtggaacga
    15181 accccagaat cacctctttc aatttcctgc ataatcaagc cataagaaat ttggtctaaa
    15241 ccggctccgc catattcttc cggaatataa ggaccaaatc caccaatttc tcctagacct
    15301 tttatgattt gtgttggaaa ttcggctcgt tgagcgtagt cttctataat aggagagact
    15361 tctcttttta cccaggcacg ggcagaatca cgaactaatt tgtgctcatc ggtgagcaaa
    15421 tcatctaata aatagtaatc aggagcttga aataaatctg gtttcatcag tatcgtttta
    15481 agtgaagcaa aagtaagtaa agaaatgtaa caaatcaacg aacgattgtt ataatttatg
    15541 taaaagccgc gaagtgattt tacattttta aataaaaatc cttggttaaa gcaatgtatt
    15601 cgtttgtgta ttcgtgtcgg ttaatttcaa tgactaattc gtctgcactt aaaacaggaa
    15661 gttcaaatcg tttgaacgcc aataaacttc gaacaattgg ggtgttgtga gagcctttta
    15721 cacgagtaac ttttatggga aaaagctcgg cttgagcaca taaatcaata aatcgttctt
    15781 cttctttatg tggaataatc actgcaaaaa ccccattctc tgataagagt aaattgacag
    15841 cttcgattaa atcttcgaaa ggcatagcat cctgaaacct tgctaaatcg cgttgctcgt
    15901 tttcactttt ataattttcg gcataaaagg gagggttaga aagaattaaa tcatattcat
    15961 cttccggttc ctccacaaat tcatccagtc ccgcatgaaa acaaaataat cggtctctcc
    16021 aaggtgagtt ttcaaaattt tcaacacatt gttcgtaggc atcttcgtca atttctaacg
    16081 catcaatttg ttcagcatta ctccgttgag ctatcatcaa ggataaaatg ccggttcctg
    16141 caccaatatc caaaacagaa aacggattat tttcaatcgg acaccaagct cccaacaaca
    16201 cactatcggt gcctactttc atagcacatc tatcttgttg aatagcaaac attttgaaat
    16261 taaaaattga cattttttta aaattttaaa ccattaagaa ttaagattca ttaagttttg
    16321 ccttaatttc ttaattctta atggttcaaa gttgttatta tggtttaaaa atttatgttt
    16381 aaagatacat ttccactaaa ccttccggta aattcatcac tactttttta ttttcgcgat
    16441 caatttttac caaaaaatgg tctatcatgg gaataagtaa ttcaacatta ccattcaaca
    16501 cttcaaataa cggttgagca gtagtatcat taatagaaac aatttttccc acaactccaa
    16561 gtcgttggtc ttccacttca aaacctatca cttcgtggta ataaaattga tttccctcta
    16621 gttttggaag aaaagataaa ggcaaatata gttcacaacc catgatttca tccgcatctg
    16681 cttcagaatt tacatcttca aatttacatc tcagaaagtc atttttatgt agggaactat
    16741 tttcaataaa aaatggaacc aaatttttgt tgaattcaac aaaaactgat tccatatttt
    16801 catataattc gggttcgtca gtatctaaat agactaaaac ttcccctttg aaactaaatt
    16861 ttttagcgat tttgccaaga tagaaacaat cttctttacg cattatcgca aggtattaag
    16921 cttctttttc ttcgtttgct tcttcagcag ctgtagattc ttccacagca ggggtttctt
    16981 cggtagctac ttcagcagcc acttcgctaa cctcttccac tgtttcttcg gcaacctctt
    17041 ctgttacttc agcagctttt gcagcctcag cagcagcatt agctcttttt tcattcgcta
    17101 ctttttcagc ttttaaagct tcagcttttg ctttcgcttc agcttttgat aaaccttctt
    17161 ttttagaagt tactttgtta gctttttcat caatccaagc cgctaatttt gcatcagctt
    17221 gttcttgtgt taatgcacct tttctaacac caccatccaa atgatgtttc aataacgcac
    17281 ctttgtatga aaggattgct cttgctgtat ctgttggttg agctccattg tgtaaccatt
    17341 gaacagcact gtccaagttt aaatcaattg tagctgggtt agtgtttgga ttgtaagttc
    17401 cgatcttgtc taagaattta ccatctcttt tggctcttga atcagcagcc acaatccaga
    17461 aaaaaggttt tcctttttta ccgtgtcttt gtaatctaat ttttactggc ataatcgtat
    17521 gattaaattt tgaggtactc gacctctgtt aattaagggt gcaaatgtat aaaatatttt
    17581 tggattagag atcatactga aaaaaaatag taaaaaacct taaatcgtat gtttttaaca
    17641 aaaaacaatg cagaatattg gagagaaaaa ctatttttgt ctttttaact tatcagtgtt
    17701 tcaaattata tttttataaa tatgaaaaaa ataatttcag tagtaatttt agccttgttt
    17761 ttcatttcgt gtgaagaaga tgttgtatta aatagcccgt ctatccaagg aaaattagat
    17821 aatgtttttt ggagagcaat agattctgaa gccttattag atgagaatgg tggattaaca
    17881 attacaggct ttggaagaaa acaaaagatg attttgcata cctcttctaa aaacaagggt
    17941 atatatgttt tagggaacag tacagctcgc tatgctaatt ttgaaagtga tattgacgga
    18001 acattattaa tttatcacac cggtaaagat attggaggcg atggtcaaat tgaaattacg
    18061 gaatttgatt ctaacaaagg aactgtgtcc ggaacgtttc gttttaatgc cataaatgac
    18121 gaaaataatc ctttaggagg cgagatactt aactttaatc aaggcgtttt ttataaaata
    18181 ccggtcattg ggtttacgga ttaagaacat tataaaatac atatttaaaa gccattttgc
    18241 tcaaaacaag atggctttta aattttaaat gagccaaaat aaaataaaag acacatttat
    18301 agtagattag aatttaattc gcttaccttt gtgtcaaata atatttttga tatgaacatt
    18361 tttgtaggaa gtcttccatt cagtattgag gaagcagatt taagagagtc tttcgaggct
    18421 tacggagcag ttgactctgt aaaagttgta actgataaat ttactggaag aagtaaagga
    18481 tttggttttg ttgaaatgcc aaacgatgat gaggcattaa aagctattga agaacttaac
    18541 ggtgctaccg ttcaaggccg tgcaattgta gtaaacaagt ctgagccaaa accggaaggt
    18601 gaaagaagaa gctttaacaa caaccgttca ggcggtggtg gtggttacgg tggtggtgga
    18661 aacagtcgtg gtggcggtgg ctacagcggt ggtggaaaca gtcgcggtgg cggaggaaga
    18721 tactagtatt ttttctttat acatttacaa aaccattctg agcaatcaga atggtttttt
    18781 gttttgttga taactaaaat tgattgctat ttctcaaaaa tgtatttttg tttctattcc
    18841 aattgctgct tttggctctt gccttttgcc taattcgctt caaaaatgta tttaatcttc
    18901 gacaccgaaa ccaccggctt acccaaacgt tgggatgcac ctgttaccga gaccgacaat
    18961 tggcctcggt gtattcaaat tgcttggcaa ttgcatgacg aaatgggtaa tttggtcgag
    19021 catcaagatt atctagtgca accggaagga tttaatattc cgtatgatgc cgaacgaatt
    19081 catggaattt caacggaatt ggctcagcaa ggtggaattt ctttaaatga agtacttgaa
    19141 aaattcaata ttgcattaag taaagctaaa tttattgttg gacaaaatgt tggttttgat
    19201 gtcaacatta tggggtgcga attctatcgg atgaatatgg attctcctat gagttctatg
    19261 cctgttttag acacgtgtac agaagttacg gcttctttgt taagacttcc cggcgggcgt
    19321 ggtggaaaat tcaaattacc aaccttaacc gaattgcatc aatacctttt tggtgttcca
    19381 ttttcagaag cccacaatgc aactgccgac gtggaagcaa caaccaggtg ttttttggaa
    19441 ttgattaaaa gagaaatttt taccaaagaa gaacttgatg ttccgattga ttatttcaaa
    19501 aattttaatg aacaaaatcc gaaagaaatt caactcatcg gattaaaaca tattaatttg
    19561 aaagaagctt ccgaagccat tcggaaacaa tttcaaaaag aacaacccaa agaagttgcc
    19621 aaaacggatt tggttgaaaa tcaacaagca ttagttgaag ttgattttgt gcatttgcat
    19681 aatcattccc aattttcagt attgcaatcc actatttcgg tagccgattt agtaaaagcc
    19741 gccataaaaa ataaaatgcc agccgttgcc atgaccgata ttggtaatat gatgggagct
    19801 tttcattttg ttcgtgatgt tttatatcat aataaatcgg ctgaagctaa aaataaagct
    19861 gccattgaag ccggagaaaa tccaaccgaa accattatca aaccgattgt aggttgcgaa
    19921 ttttttgtct gcgaaaatca caaagataaa accagaaaag acaatggtta ccaagtggtt
    19981 ttgttggcta agaataaaaa tgggtatcac aatttagcca aaatgtcctc cattggattc
    20041 acagagggtt tttattatgt gccacgaatt gacaaagaag ttattcaaaa atataaagaa
    20101 gatatcattg tattgtccgg aagtttagcc ggagaagttt ccaataaatt attgaatatc
    20161 ggtgaaaatc aagccgaaga agccctgctt tggtggaaaa ctgaatttgg tgatgatttt
    20221 tacatcgaac tcatgcggca taaccaagaa gatgaaaacc gtgtaaatac ttcgttgatt
    20281 tcattagcca aaaagcatga cgtaaaaacg gtggctacca acaatgtttt ttatgtggat
    20341 aaagaaaatg ctaatgctca cgatattttg ctttgtgttc gcgatggtga aaaacaagcc
    20401 actccaatag gaagaggtcg cggttatcgc tacggtttgc ccaatcagga atattatttc
    20461 aagtcgggcg acgagatgaa aaaactcttt gccgatttac cggaagccat tttaaatatt
    20521 aatgaagttg tcgataaaat agaaattttc gatttggctc gtggtgtttt acttccaaaa
    20581 tttgatattc cacaagattt tttagttgca gaagacgaac aggatgctgg taaaagaggt
    20641 gaaaatttat atttgagaca tcttaccttt gaaggtgcaa aaaaacgcta cggaaccatt
    20701 acgcctgaaa tcgaagagcg attggatttt gaattaaaaa ccatcgaaaa cacaggttat
    20761 cccggttact ttttgattgt tcaggatttt attagagccg ctcgtgaaat ggatgtttca
    20821 gtgggacccg gacgtggttc ggctgccggt tcagtggtag cctattgttt atggattaca
    20881 aacattgatc ccttggaata cgatttgctt tttgagcgtt tccttaatcc tgatcgtgtt
    20941 tccatgcccg atattgacat cgattttgat gatgaaggtc gtggtcgagt gatgcaatat
    21001 gtaattgata aatacggttc aaatcaggtg gcacagatta ttacttatgg taaaatggca
    21061 accaagtcat ctttgagaga taccgctcga gtgctcgatt tacctctttt tgaagcggat
    21121 aaattagcta aattaattcc gggaatgatg cctggaaaat ggagtttagc ccgattttta
    21181 tccgattcga ttgaagaagt aaaaaaagct gttcgttcag atgaatttga taatattaaa
    21241 gaattaattg cgattgccaa cgaaggtgaa ttagccggag aaactatcca acaagcaaaa
    21301 gttttggaag gttcgttgcg aaataccgga attcacgcat gcggagtaat tattacacca
    21361 agtgacatca ctaatttcgt tcccgtagcc acagccaaag attctgaatt atatgtaacc
    21421 caatttgaca actcagtagt ggaaagtgcc ggattgttaa agatggactt cttgggtttg
    21481 aaaaccctta ccttaattaa agacacagtt aaattagtta aatacagaac cggaattgaa
    21541 cttgatccgg atgcttttcc aatcgatgat gtcaaaacat atgagttatt tcaacgtgga
    21601 gaaacagttg gaattttcca atatgaatcg gttggaatgc aaaagtacct tcgtgattta
    21661 aaacctaccg tttttggaga tttaattgcg atgaatgctc tttatcgtcc gggaccattg
    21721 gaatatattc cgtcttttgt tcttagaaaa aatggacaag aagaaataaa atatgactta
    21781 gatgcttgtg cagaatattt aagcgagacc tacggaatta cagtatatca agagcaagta
    21841 atgcttttgt ctcaatcttt agccgatttt tcaaaaggtg atgccgacgt tttgcgtaaa
    21901 gcaatgggaa aaaaagatcg agcaacgttg gataaaatga aaccaaaatt catagaacaa
    21961 gcagcatcca aaggtcacga tgcaactgtt ttagaaaaaa tttggaaaga ttgggaagcg
    22021 tttgcgagtt acgccttcaa caaatcgcac tccacttgct acgcttggat tggttatcaa
    22081 accgcttttc ttaaagctca ttatcctgcg gagtacatgg cggcggtgct ttctaacaac
    22141 atgaacgata tcaaacaggt ttcgtttttt atggaagaat gtaagcgaat gggtttgcga
    22201 gtattaagtc ctgatgtcaa cgaatcattt tataaattca ctgttaatga caatggtgcg
    22261 attcgtttcg ggatgggagc tattaaagga gttggtgccg gagctgtaga aacaatcgtt
    22321 caaaaccgat tgaaagaagg aaaatataag tcaatttttg atttggctaa acggattgat
    22381 ttacgtgccg ccaataaaaa agcctttgaa aacctagctt tagcaggtgg ttttgatggt
    22441 tttacaacaa cgcatcgtgc tcagtatttt catcatgatg gggacaatat cacttttttg
    22501 gaaaaagcca ttcgttacgg agcaaaattt caggaaaatg aaaattcagc tcaagtaagc
    22561 ttgttcggtg aagcaagtga tgttcaaata gccgaaccgg ttgttccgcc ttgtgtcgaa
    22621 tggaatacaa tggaaaagtt agccaaggaa aaagaagtag tcggaattta tatatccgga
    22681 catccgttag atgattttcg ttttgaaatg aaatatttct gtaattctaa attagaccat
    22741 cttaaacatt tagaacagca tcttaataaa acactctctt ttgggggaat aattacaagt
    22801 gtacaacatc gtgtggcaca aaatggaaaa ggctgggcat cttttatact tgagggttat
    22861 gatgaaagcc acgaatttag gatctacgga gaggaatatt taaaattcag gcattttatc
    22921 attcaaaaca attttacatt tattaaagta atggttcgcg aaggctgggc caataaagaa
    22981 accggtaaaa aaggggatgc cagaattcaa tttcaattga ttcaatattt acaggatgtg
    23041 ttgcctcaat ttgctaaaaa gctcacgatt cagatgaata ttatgatgat tcatcaaaat
    23101 ttaattgctg aattaaatga tatttttaaa acttataaag gggataatac ggtaactttt
    23161 gaaataatgg aattagaaaa agtgaaaaat gaaatcgaaa taaaaccggt tttaattcca
    23221 gttgatgagg atttagatat ggaaaattta gaagacattg aaattgaaat ggaagctcca
    23281 aaagagaatg ttaaagtcgt taatcaattg gttatgccga gcagaaaatt gaaagttcaa
    23341 attagcaatg aactgttatc cgaattagaa aaaaaacaaa ttgattttcg attaaattaa
    23401 ttatcaattc cacaaataca ttcataatca aaactaattt tcaattaaca tcttattaag
    23461 ttttaaattt ttaaatttgc agagttaaaa aaacaagaac gttaatctaa aaaaaatata
    23521 taaaaatggc attagcaata acagacgcta cttttgatga agtagtttta aaatcagaca
    23581 aaccggtagt agtagatttt tgggcagctt ggtgtggacc ttgtagaatg gttggaccag
    23641 ttatcgacga aattcacggg gaatatgaag gtaaagcagt agtgggtaaa gtagatgtag
    23701 atgcaaacca agaatttgct gcaaaatatg gtgttagaaa cattccaacc gtgttaattt
    23761 tccaaaatgg agaagtagtt ggtcgtcaag taggggttgc tcctaaaaaa acgtatacag
    23821 atgcaattga tgcattgttg taataagtaa aaaaataatt attaataaag gtctgatgta
    23881 aatcaggcct tttttgtttg ttaaattaat tttcaaatgg aatcattttc aaattttcaa
    23941 attatctttg agctatgaag atagaagatc aaaaagaaat aatttctggt tttaaacacc
    24001 tcgaactact tgccaatcaa gttgtagaag gttttatttc cggtatgcat aaatcaccat
    24061 ttcacggttt ttcggctgaa tttgcagaac ataaagtcta taatccaggc gaaagcacga
    24121 aatatattga ttggaaattg tttgccaaaa ccgatcgatt gtataccaaa agatacgagg
    24181 aggagaccaa tctgcgttgc catttgatta ttgacaattc ttcttcgatg cattatccga
    24241 agttaaaaga aggggagcca ttttacgaaa gcaaaattgg tttttccgta ttggcatctg
    24301 ctgttttgat gaatttgctt aaaaagcaac gtgatgcagt gggtttgagt gttttttcgg
    24361 atgcatatga atactatgct cctgaaaaag gaagtgatcg tcatcatcgg atgattctga
    24421 acaaattgga agacttgttg gaaaatccaa aagtgccaaa acctacagaa acagttactt
    24481 ttctacatca gattgctgaa aaattacatc gtcgttcgat gattgttttg tttacggata
    24541 tgttccaaac gggagaagaa acagcattgt ttaatgcctt acaacatttg aaacatgaca
    24601 aacataaagt agttttgttt catgttattg atgaaaaaag agaactcaat ttcgattttg
    24661 ataatgcacc aagaaagttt attgatgtgg aaaccggtga atcagtaaac ttatttgctg
    24721 aaaacatcaa agaaacgtat gaaaaaaatg tggaatccta ctttaaaaaa gtagcaatga
    24781 catgtgctca aaataaaata aaatacgttc cggtcaatgt tggagccaat ttcgaaaaaa
    24841 ttttaacgac atatttagtt gaaaaacaaa tgtttgggta gtcgctaaat gaagtggcta
    24901 aaaaaagtaa aaataatgtt tgcagatatc gaattccgtt gtatatttgc aaccgcaata
    24961 aagcgaaggt ttggtagttc agttggttag aatacatgcc tgtcacgcat ggggtcgcgg
    25021 gttcgagtcc cgtccagacc gctaaaattg ggaaaataaa taaaagcact ttggaaacaa
    25081 agtgcttttt tgcccaaaaa agcatttaaa atagttgtgt tgtttaggac gactgaaagg
    25141 aagtcctggt ttagtagtta gaccaatgaa aagctttccc gttctcggga tagctttttt
    25201 gctttgtata ttatcctaat gatagaaggc tccagcgaca gcaatccaac aaagcaatat
    25261 taataaagaa tttgttgatt aatatgaaaa atcaccgtca ctttttaatt tataaacctt
    25321 acggctacct cagtcaattt atttatgaac taaaaagaac aaaaaaatta ttgggtgaac
    25381 tacatgattt tcctcttgga acaatggcta tcggtcgctt agacgaagat tccgaagggt
    25441 tgctcattct caccacagac ggtatgatga gtgaagttgt tagaagcaaa aaggtagaaa
    25501 aagaatacta tgcacaagtc gatggaatta tcacaaagga agccgtcgaa caacttcaaa
    25561 ccggagtcct aattggtttt aaaggaacca aatacaagac taaaccttgt caagcaaaat
    25621 taattgccga cccaaatttt ccattgcgat cacaaaaaat tcgtgacgag cgtcacggac
    25681 caacctcttg ggtttccatc acacttaacg aaggtaaatt tagacaagtg cgaaaaatga
    25741 cttctgctgt tggatttcca acattacgtt tgattcgggt acgaatcgga aatttccact
    25801 tagataaatt gcaatcggga gaagtgaagg agttagatag ccttttagaa tgaatttaat
    25861 tgagagaaat cacctattca accaccaaat tccaccattc aaaatcatag caaaacttac
    25921 ccaaaggaag taaggtatca ataatttact tgccaaagaa tctatcgatt taaaaacttt
    25981 gatggtttct acaatcatca accaaagtaa taagatttca agcaaagcta aaaacggatt
    26041 gcacattcca aaaaataaaa aactccacaa cgcattcaaa atgagttgga tggtaaaaac
    26101 ccaaagtgct tttttgacta aaatcggaac tgtttccatt ttgctccaaa tcaaccctgc
    26161 actcacaccc atcatcacat aaagaattgt ccaaaccggt gcaaatagcc aattgggtgg
    26221 attgaaaaaa ggtttgttta aggtggggaa ccaagtggtt attgaagatt gagttgcaaa
    26281 tccggaacca taaccaaccg ctaaacaaac ggcaacggca atagcaattt ttactgattt
    26341 tgacatgatt atttttattc aaaagtaaca attctgactt caaaaacaga cctaataaac
    26401 caaaaaatgt atctttgccc aaaaataaac ggcatgttat ctccttcaga ttttattcct
    26461 caaaactata cacaaacagt tgaaaaggga agttttcaat ggtctgctcc cagcaacatc
    26521 gctttggtga aatattgggg gaaaatgaaa aatcaaattc ctgctaatcc atccattagt
    26581 tttacattaa aaaattgtaa aacaataacg aaactgactt tttctaaaat tgaaagaaat
    26641 cctaattttt catttgattt attatttgaa ggaaaagcaa aggaaacatt ccgacctaaa
    26701 attcaaaatt ttttggaacg agtggaagtt tatttacctt ttttaaaaga ttatcatttt
    26761 acgattgaca ctcaaaacac attcccgcac agttctggaa ttgcttcttc agcttccgga
    26821 atggcagcat tggcagtcaa tttcatgagt ttagaaaaac agttaaatcc gctaatgaca
    26881 gctgaatatt ttaatcgaaa agcttcattt ttagctcgat taggttccgg aagtgcctgt
    26941 cgaagtgtaa aaggtaaagt tgttatttgg ggaaatcatg aggaaaccga aaacagttca
    27001 gatttgtttg gtattgaatt tgttgaactt cattcaaatt ttgaaaatta tcaagatact
    27061 attttattgg tagataaagg cgaaaagcaa gtttcaagca cagtaggaca tgatttgatg
    27121 aataatcatc cattcgctga aaaaaggttt gaacaagcac atcaaaattt gtcaaaaatt
    27181 aaaaatattt taaaaaacgg tgatgtaaat gaatttgtaa aattagtaga aagtgaagca
    27241 ttaacactac atgcgatgat gatgacttca atgccatact ttattttaat taagcccaat
    27301 acattggaaa ttatcaataa aatttggaaa tttagagagc taacaagtat tcctgtttgt
    27361 tttacattgg atgccggagc aaacgtacat gttttatatc ccgaaaacgt tcgcgaagaa
    27421 attttgcaat ttattaagaa tgaattagtt gtgcattgcc aaaacgagca gtatatttgc
    27481 gacgaaattg gcagtggaac tatccaactg aacatttttt gattatcttt accaaacata
    27541 aatagaaaaa tttatatgaa aggacctctt ttttactcaa aaattcttct ttttggagaa
    27601 tacggaatca tcaaagactc aaaaggactt tctatcccat ataattttta taatggtgcg
    27661 ttgaaggtta gtgaaaaccc atcgaacgaa gccattaaat cgaatgaaag cctacgcaag
    27721 tttgctttgc atttagagca attgcaagcc gagcaatctg aattggtaac tttcaatcta
    27781 gcattactca atagcgatat tgaaagaggg atgtattttg attcttccat tccacaaggg
    27841 tatggcgtag gaagtagtgg tgctttagtt gctgctattt atgataaata tgccaataat
    27901 aaaatcacgg ttttagaaaa tttaacccgt gagaagttat tacaattaaa ggctattttc
    27961 tcgcaaatgg aatcgttttt tcatgggaaa agttcgggtt tagatccttt gaacagctat
    28021 ttaagtttac cgattctgat aaattctaaa gataatattg agccaacagg cattccctca
    28081 caaactacca acggaaaagg agctgtgttt ttgttagatt ccggtataat tggggaaact
    28141 gctccaatgg taaatatttt tatggaaaat ttgaaagacc aaggctttcg aaaaatgctt
    28201 aaaaatcagt ttgttaaaca taccgacgct tgcgtagaaa actttttgca aggcgatatg
    28261 aaatcacttt tttcaaatac aaaaaaattg tctaaagtag ttttgaataa tttcaaaccg
    28321 atgattccgg aacaattcca caacatttgg caaaacggaa tcgacaccaa tgattattac
    28381 ttaaaacttt gtggttcagg tggcggaggt tatattcttg gttttacaca agaccttgaa
    28441 aaagccaaaa aatcattaaa ggattataaa ttggaagtag tttatcaatt ttaatagctt
    28501 taaataaaga tattcaggat tctcattctt gattttttat gaactatggc attatccaga
    28561 aaaaataaat tgattttaat gaaaataatc agtttgtttt cagtggttag aggctataac
    28621 attccggtta tcattttagc acaatattta gcggcaattt ttattttatc tacagattta
    28681 agagctcttg atgtcttgct cgattttaat ctttttgtca ttgtatttgc ttcagcaata
    28741 actattgcct ctggttatat aattaatagt ttttacgata gtaaaaaaga tttaattaac
    28801 aaaccaaaca aaacgatgtt agaccgtttg gtttctcaaa aaacaaagtt gcaggtttat
    28861 ttcggactca attttttagt ttttttgatg gcaatgctag tttcttggcg agccgcgtta
    28921 ttcttttcaa gttatatttt tttaatttgg ttttattcac ataaaataaa aaagtaccca
    28981 attgtcggaa acattatggc ggctttgtta gctgttttac cctttttcgg catattgctt
    29041 tattatttta aaatagatgg ccttttagaa aatgttcgaa acgagaaata ctatgttatt
    29101 tttgctcatg cttcttttct ttttttactt attcttatta gagaaatgat aaaagattta
    29161 gaaaatatca aaggcgatat ggctcaaaat tacagaacac ttccggtcat tttaggagaa
    29221 tcattttcta aaaaagtgat cactttttta ataatatgtt ccattttccc cgtttatatt
    29281 ttaattgaaa tttatgatgt tggttacatg gatatttatt tttatgtcag ttttattatt
    29341 ctgattttct ttataatgaa attatggaat tcgaattcaa aagaaaatta tttgctttta
    29401 cataatatta ttaaattcat aattgttgcg ggtgttttta gtattattct tatcaaacca
    29461 tccgtattga ttcatggtcg ggaagtttta gcgatttagt aaaaaagtct ctttttattt
    29521 gattttgaaa caagtcaaca aaccaatttc gaattccgta tctttgcaaa aattttcaag
    29581 gatgaacaag aaagagggaa gcaacagaaa atcaacttcc aacagaaata caaaaggaaa
    29641 accaacgggt tttagtaagc ctaaaccggc catggctaag cgttcgcaaa aacctaaaaa
    29701 agtaactaca gatactccgc cacccgcagc caagccaaaa gtgaaatcag atgatattcg
    29761 tttgaataaa tacattgcta attccggtgc atgttcccgt cgtgatgcgg atatttacat
    29821 tcaatccgga aatgtaaagg tgaatggcga agttatcatt gaaatgggtt ataaagttaa
    29881 acccggtgat gtggtcaatt ttgatggagc aacaattgtt cctgaaaaaa aggaatatat
    29941 tttattaaat aaacccaaga atttcactac ttccgtggaa gatgatcgcg atatgcgaaa
    30001 cgtttttgaa ttgattaaaa atgcttctca ggcaaaattg caggcaatcg ggcgtatgga
    30061 taaaaatact accggtttgt tgttatttac aaacgacacg gacatgatac gtaaatttgg
    30121 tttacccaat caaaagtcac caaaaatcta ccaagtttca ttagataaaa atttaaaatt
    30181 tgaagattta gaaaaaattg cgggtggaat atcttttgac gagcaccgtt tgtatgttga
    30241 cgaaatcagt tacattgacg atcaaccaaa aacggagatt ggcattaaac ttcgcacacc
    30301 aaatgttaaa gttgttcggg caatttttga aaaattcggc tatgatgtta taaaagttga
    30361 tcgcgtatct tttgccggtt taactaaaaa gaatttgcca cgaggtaatt ggcgtgcctt
    30421 aacggaacaa gaaatcataa acctaaagaa tttttaacat gaattcacag gaaatcatgt
    30481 cgattgctta taaatggttt gaggcattca ataatcataa tttagaacag ttgttgtcac
    30541 tttatgatga taatgcccaa catttcagtc cgaaattaaa aatcagaaaa cctgaaacaa
    30601 acggtttagt gattggaaaa gaagcgatgc gtgattggtg gaaagattct tttgatcgtt
    30661 tgccgtcttt gaattataaa gttacttcat taacggccaa ttcagatcgc gtttttatgg
    30721 aatatgttcg ctctgttgat aatgaagaag atatgttagt ggctgaggtt ttggaaattc
    30781 gagacaaaaa aattgttctt tcgagagtgt accacggtta actaaaaaat cccgaaatga
    30841 acaatcatct cgggattttt tttggaatta acaataacct gatttatttt gactgcgttt
    30901 ctaaaccacg ttttttcaaa agtggttcta tacttggttc gcttcctcta aattttttat
    30961 acaactccat cggttcttgc gttcctcctt tttccaaaat attaattcta aatgaatctg
    31021 ctttttcact gtcaaaaaga gaggttttct taaattggta aaaagcatct gtatctaaaa
    31081 cacctgacca tatatagcta taatatcctg ctgaataacc tccggcaaaa atgtgactga
    31141 aataatttgc tcgatgacga ggaattatgg aaggaattaa tccgattttt ttcatggatt
    31201 cattttcaaa agcttcggca gtacctgtaa ttgtagtggt agaagtatga aaatccatgt
    31261 ctaaaaaggc agatgctaag tattcaacgg tagcaaatcc ttggccataa gttcctgaat
    31321 tctctaattt ttgaatcaag gcatccggaa taacttcgcc cgttttataa tgtttcgcat
    31381 acatttttaa tacggcagga tcagctgccc aattttccat gacttgtgat ggtagttcaa
    31441 caaaatctct ataaacattt gttcctgcta aacttttata cgacacattc gataataaac
    31501 cgtgtaaggc atgaccgaat tcatgaaaat aagtagttac ttcatcaaaa gtgaaaagtg
    31561 ccggagcatc agcggttggt tttgaaaaat tgcaaacaat tgaaattatg ggtggaatgc
    31621 gtttatctcc ctctttttgt tgaggtttgt aggaagtcat ccatgcacca ccttgtttag
    31681 atgctcttgg gtaaaaatcc atataaagaa tacctaaatg tttgccattt ttttccatta
    31741 cttcccaagc agtaacctcg tcatgataag ttggaatgtt ttttaattct ttaaattgta
    31801 aaccatataa tttatcacaa accataaaaa cgccattagt aaccgcttct aaactgaaat
    31861 aaggacgaag ttcttgctca tctaaatcaa atttttcaat tctaattttt tcgctgtaat
    31921 aacgccaatc gtaaggttga acattatctt ttataccttc agccatcatc atttgcttaa
    31981 tttgagcttc ttcaactttt gctttttcta aagcaggagt ccaaagttga ttcaaaaaag
    32041 tcatcacttt ctcgggtgtt ttagccatac tttcttccaa aacataatga gcatgtgttc
    32101 tataacctaa taatcttgct ttttcagctc gtctattcgc tatttgaaga gcaattgcat
    32161 tgttgtcaaa ttcatttcca ttattacaac gatttttata agctttccaa atttcttccc
    32221 gaagttgtct attcgaacaa tattgcaaaa aaggcatcac acttgaattg gaaagagtga
    32281 taacccattt cccttttttt cctttccctt ttgcggtttc tgcagcagct gtgattaatt
    32341 cttccggtag accggctaat tgttcctttt tgtttacaat atattcgtag ttgttggttt
    32401 cagctaaaat attttgatca aattttaatg ttaaaacagc aatttcatta ttgattgcac
    32461 gaagtttttc tttatcagaa acatttagat ttgcaccatt acgaacaaat tctttatagg
    32521 ttttttccaa taatttattt tgctcaagcg taagattcaa ttggtctttt tgattccaaa
    32581 tcgttttaac tttggcaaat agtttttcat ttaaattaat attatcagaa tgagttgaaa
    32641 ttaatggagc aatttcatta gcaatttttt gaatttcgtc attcgtatta gctgaattta
    32701 tattgtaaaa aactgtcgtt accttagtta aaagttcgcc agaattttcg agtgctaaaa
    32761 tagtattgtc aaaagtcgga atatttttat tgtttgcaat tccatctacc tcccgacgtt
    32821 gaactaaaat tccttcttga atggccggtt tatagtgttc gtttttaatc agatgaaaag
    32881 gaggaacatt aaaaggagtt ttataggaac tcaaaaaagg attacttgcg taaattggtt
    32941 tcaattcctg agcctctact tgaaaatttg acaaacccat tgttagtata attaaataaa
    33001 aagtgtattt cattaataaa ttatttaaaa gaaagctaat gtagttattt tttaaaattt
    33061 actttttgga ttaacttatt tttaagacaa aagtcagcat tggccgcata tcattataaa
    33121 ttaaatttaa tcacaatcat ttaggcataa taattgttca attttaaaat taaatttcta
    33181 cttatttgat ttttttaata tattttaata aaatgtaaaa tcttaaataa atttttatga
    33241 aaatggttaa agtgttattt gtttttttct ttagtatttc ctttttagtt tcctgtgctg
    33301 atgatattga tgataatatt gtttcagcac cttctgattt ttattattta ggagaattac
    33361 cgattccttt ttatacgaat ggaaatactg attttcctaa tatagattgg ggaaatgaat
    33421 ctggtgtttt tactttaaat gaaaattatc agggagttag tattaatcca tctaacggag
    33481 tattatcttg gaacgaggat ttaccgttgg gtgaaaatat tatttctgtg actgccacaa
    33541 attctgctgg ttttgcagta accagtgtat tgtttctgca tcagtttgga ggagaattta
    33601 ccggaggata taattcagac cctacttcaa caacagtttc agcctctaat ttagttatta
    33661 attttaatgt taacggcaca ttagtcataa cagataatgg agaaacagta aatggagcgt
    33721 ggagttttaa tccgcaagga aaattagttt gtaattatat gttatcatcc ggaagctatc
    33781 aattaattta tgatttgact tattcggtta ctgtaaatcc ttttttagaa ggatacaaaa
    33841 gattagaagg gagtacggtg aatcttggct tttcaagaat ggattattaa ataatactgc
    33901 atagatttta tgaatggacc atctgttgaa acgtatggtt tttttatgac tctttctaag
    33961 tatttccaaa aaaaagccca aactttcgtt tgagctttgt gcgggtaata ggactcgaac
    34021 ctacacacct tgcggcacca gatcctaagt ctggcgtgtc taccaatttc accatacccg
    34081 cgggcgaata aaacagtagc tgtttcaata agtgggtgca aatatagaga taattttgaa
    34141 ttttaaatta tgaatgatga atttttttag ccctatattt tttgtatttt tgaactttat
    34201 catttttact ttttacaatg gacaacataa aaaactacgt tcaacaaaat aaagaacgat
    34261 ttattagtga actgattgat ttacttaaga ttccttcagt aagtgctgat agtgcttact
    34321 cacaggatgt tattgacact gcacatgcag taaaggcaag tcttgaaaaa gccggttgcg
    34381 atttcgtaga attatgcgat accccgggat atccaatcgt ttatggagaa aaaacaatag
    34441 acaaatcatt gcctaccgtt ttggtttatg gacattacga tgttcaacca cccgatccga
    34501 tggatttatg gacatcacca ccttttgaac cggttatcaa aactactgaa attcaccccg
    34561 aaggagctat ttttgcccgt ggttcttgtg atgacaaagg tcagatgtat atgcatgtga
    34621 aagcattcga atatatggtt gctaataatt gcttgccgtg caacgttaaa ttcatgattg
    34681 aaggagaaga ggaagttggg tctaaaagtt tggggtggtt cgttgaacgt aaccaagaaa
    34741 aattagccaa cgacgtgatt ttgatttccg ataccggaat gatttccaat cagcaaccat
    34801 cgataacgac tgggttacgc gggttaagtt atgtagaagt agaagtgacc ggaccaaatc
    34861 gcgatttaca ttccggattg tatggcggag cggttgcaaa cccgatcaat attttggcga
    34921 aaatgattgc ttcattacac gacgaaaaca atcatatcac tattcccggt ttttatgaaa
    34981 aagtggaaga attaaccact gccgaaagag ctgaaatggc caaagcaccg ttttctttag
    35041 ataattataa aaaagcatta gacattgcag acatatatgg tgaagccggt tatgtaacca
    35101 atgaacgtaa ttccatccga cccacattag acgttaacgg aatttgggga ggttatacag
    35161 gagaaggtgc caaaacagtc attgccagta aagcctatgc caaaatttct atgcgattag
    35221 ttccaaatca agattgggag gaaatcactg aattatttaa aaatcatttt gtgagtatcg
    35281 ctccaaaatc agtgaaagtc aaagtgacac cacatcacgg aggccaagga tatgtgacac
    35341 ctatcgacag catcggttat aaagctgcaa ataaagcgta tactgaaact tttggtgttc
    35401 cagccattcc ggttcgttct ggtggaagta ttccaattgt agcactgttc gaaaaagaat
    35461 taaaatcaaa aactatatta atgggatttg gtcttgacag cgatgccatt cattcgccta
    35521 acgagcattt tggaattttc aattacctca aaggaatcga aaccattccg ttgttttata
    35581 aatatttcgt ggagttatca aaataaacca tagaaaaata ttgtaaaaat ccgtttattc
    35641 aattgagtaa acggattttt tattgccccg atttggnnnn nnnnnnnnnn nnnnnnnnnn
    35701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35761 nnnnnnnnnn nnnnnnatca atcattttac tgaactcgct tcagtagtaa aatcatcaat
    35821 catcatgctc aaaaaattct tcctgctttg ttcgggttcc gacaagcaaa tcatcgaaag
    35881 ttgttctgat ggcgaacaaa acaaatatgc cggaatcggt gcgacagttt tctttacggc
    35941 agttatggcc tttattgctg gcagttatgc tctctatacc gttttcgata acatttatac
    36001 agccttgttt tttggattgg tttggggttt acttattttt aacctcgatc gttttattgt
    36061 ttccactatc aaaaaaagag acagttttct ggatgaattc attcaagctt cacctcgaat
    36121 tgtgttggcg gttatcatcg ccattgtcat ttcaaaacca ttggaaatca aaatttttga
    36181 aaaagaaatt aatactgttt tgttgaaaga gaaaaatgaa atggccttga ataataaaaa
    36241 acaagtagct aattttttcc aaaccgatgt ggatcaaaat aaagctcaaa tagacagttt
    36301 aaaatcagac atcttgaaaa aagaaaaaga agtcaacgat ttatacgccg tttatatcac
    36361 cgaagcagaa ggaactgccg gtaccaaaaa acttggaaaa ggtccggttt acaaagaaaa
    36421 gcgagaaaaa cacgatgcgg ctttacagga gttagccact ttgaaaacaa ctaatcaaac
    36481 aaaaatcatt gaattagagg cgaaaggtaa aacccttcaa accgatttgg ataaaaaagt
    36541 agccgataca caacctatta tcgatggttt tgacggatta atggctcgca tcaatgcctt
    36601 ggataaattg ccttggttgc cctctttttt tattatgtta cttttcttgg ccatcgaaac
    36661 atctccgatt attgctaaat tattatcgcc aagaggtgag tatgatttta aacaggaaga
    36721 tggagaattg gcggtgaaaa atgtattagc tcaaaacaaa taccagagtg atttgcaaaa
    36781 acaaaccgat gcttctattt atgataaagt atatgccgat attcgagaag acaaaggttt
    36841 atatgattac aagaaaaaaa gagccattga attgttagaa ttgcaagcgg atggttttgt
    36901 agagaagcag cgaaagagtt tataaattta aaaaggcaat ggaaagtaaa tttccattgc
    36961 cttttttcaa ataaatacta aatactacgt aaaaattatt tgagttctgt caccatcaca
    37021 taattcgtaa aattcaccaa tagtgatgat atccgttact tcatctgaca aatcttcttt
    37081 agtcaattta aacatgtcaa cagcaagttt acacgcgtaa attgttccgc caccggcagt
    37141 aatcatttcc aaaaactcat caacaggtgg cattcctaat tcatccattt gttttctcat
    37201 catgcttgaa acagctgatt ctactcccgg taaaccaccc aacattgttg gaaaaggtaa
    37261 accaccgggc attttcatgg caggattacc tgtagtggca gtgtggagat gattcatcga
    37321 tttttttgta atgccttcaa gtccgaaaaa agtgaaaaat aaatttgttt cgatgccttc
    37381 cattacggct ccattagcca taaccaagga ggcataaaca tcttccagat ttcctttggc
    37441 acaaattatg caaacttttt cgagtggttt tttttctttc gtttccattt tgaaaaattt
    37501 aagttaaata caactttttg gtcgtggtat tcctgccatt ttgctggaaa tttttaatgg
    37561 cccttttgga aacaaagcat acaattcttt gatatccact ataccacttt ttccaacacc
    37621 tcgaattgtt aaagcaatat cttttttatg ttggtcttgc aaataggcta aaacctcaaa
    37681 atgtttggga gttacttcct ctattccttc ttcttttcca atttcaattg caatttcttt
    37741 attccattgg gataaatctg ttaaatagcc attgctgtca acacttacag attttcctgc
    37801 gattactttt tccatcttat tattatttta aattaaacat tatttttagt taaaattctt
    37861 tcctgctttg ctcatttttg gtgtgatgaa cggtaatttt cttgcgggaa gcaacatgtt
    37921 ccaataaatc catcggaagg ctaattttcc ccaatgattc aatcgcgatt ctgtcaataa
    37981 atttaaaggt ccaattcctg cgaatggaaa cttgcctttt acgggttcat attcgtaatt
    38041 gaaatcaatt aaaagtgctt ttccgtgacc tgtttcgata aaacaatttg catgcccgtc
    38101 aaagtccggt tttaattctt tcccgtcaat atacaataat atgttttcgg ttaatatttc
    38161 tgcttgaaaa tgagccacag aaccggcttt tgaagcagga acattggtac aatcaccaat
    38221 agcaaaaata ttttctttca cagttgtttg taaagtgtgt ttatcagtag gtacatagtt
    38281 catatcgtcg ccaaaaccgg atctagccat tacctcatca cccatattag tcggaatggt
    38341 tactaacaaa tcaaaaggca cttcatcacc tccgtaatct acaatttttc cattttctcc
    38401 atcgatgtgt tccacggcaa aatcaggtac aattttgata tttttatcct ttaataaata
    38461 tgttaaagca gttgtgcatt tttcttttgt aaatgcaccg gaaagtggag taacataagt
    38521 aatttccact ttatcacgca ttcctttatt gaggaagaat gaatcggcta aaaaagcaaa
    38581 ttctaaaggt gcaaccggac atttaatagg catttcggta atatgaacta ctaatttacc
    38641 acctttccat gttttaagtt tgtcgcgtaa agctaacgca ccttcgaagg tataaaaatc
    38701 aaagattgtt ttataccaat aatctcctaa cattccttcc acttctccgg gtgccgtttt
    38761 cgttcctgta gcaattataa gaatatcata cttcaatttt tctccggtat ctaataaaac
    38821 ggtatcggag tctttatcta tgctttcaat tttttcacga acaagcttca ctccttttgg
    38881 aataaattca tcgattgttt tttttacatc ttccggtttg taggtgtcaa aaggtaaaaa
    38941 caaaaatccg ggttgataat aatgtgtttt gtattgatca actattgtga ttttccactc
    39001 tgatttattt aattttttaa ttaaatggct cgccatcata gttccggctg ttcctgctcc
    39061 aagtattact aaatttttca ttgtttataa attatgtaat gttaaatctc ttatcaaagt
    39121 tacaattcta ttaaaattta ctatgtgaca attgttacaa ttaaatataa aaaaagtgat
    39181 tatatcttta atgaaatgaa gaattacttt ataaaattga tttgttcaat taaacaaaaa
    39241 tgaagatgaa aaggagttaa tgaaaagcga ttcctttgga gagaaaccgc ttttcaaact
    39301 tcaattacaa tctgttagtt tgtttgtttt ttactgaatt tatatgaaat accaaccatg
    39361 attagatctg ttgtcatatc ataagaaaca ttactgcccg gaatggcacc ataaggattt
    39421 gaacttgagg caaacattgg attaagcaat tgtcctgatg ttttgcctga acttgtacca
    39481 tgatgatata cagcatctaa agaaatgttc tctccagcta agtaactaaa tccaagttga
    39541 taggcgtttt taatcactgc agttgccgga atattaaaca tggcaacatc actactaatt
    39601 gggtttgagc tataggtata ccctaaacgt aaagggaatt tattcactcc tttatattga
    39661 ataccggctg atactattga tatattatcc caaccaaatc cagcaactga agctgttggt
    39721 gtccatcctg tttcagaaaa accatctgtg ttttcataat caaccattct gtaatctaaa
    39781 gcaaaatcaa aatcgctttt agaataacct agaccaaatg atagtattgc tggataatcc
    39841 atgttgaatt tatttgtacc ggttgaatta tccaaatagg tatttttaaa ttcaaaatca
    39901 ctgaaatatt gtgaagtttt ataagaagca cctgctttaa atccttttcc ggaattataa
    39961 aataatccaa tttgtgcacc aaatcccgtt gcatttgctt tgtcagtaga tggataacct
    40021 gcttgagaag gatttgcagt tggattaggc attaattcta atgttgcata gttaaatgtt
    40081 ggttcaatac caattgaaaa tttttcagat aattcataag cccaggtaaa acctacttgt
    40141 aataacatat aatctgattc aactcttcca aaaccgccca tactttgagg catatttatt
    40201 ggatttgttg tgctttcagg aaatgtaact ccaaatccac taatcccaaa cgcagaggca
    40261 ccaaacgtgt gtttgcttcc ttctttgccc caaaccatag ctaatgccgg catcggcgaa
    40321 acacctcgat catcttttgt agaaccactg aaaaaattac cggtaggttg gcctaattcg
    40381 ttaaattcag gaacggtaga aaataattcc ggggaagaat aaaacattcc aatatcaaac
    40441 ttaattattt tgtcattaaa agcagaaata gaagctgggt tccattgtaa tgcaccggaa
    40501 atatctaaag gctgtgcggt tgatgcacct cccattgaca tgttaacagc cccaacacct
    40561 tgcatgatgt gaccgctttg tgaataagca aaagatgata gtagaaataa acaaacgggg
    40621 aacaaaattt ttttcataat cgtatatttt aattagtagt aaaaacaaat tttattgatt
    40681 tgctactata aatttaagtc attataattt ttttatttgt aacaattgat acatataaaa
    40741 aagatcacga actaaactaa aatattgata atcaatacta taaaattgaa acataatttt
    40801 ataatttaaa taaaatatgt tatcataaaa atgataaaaa tcaggttttt gtaaatttta
    40861 aagaaagatt ttggaacaga aaaatcccga taaacgataa attcgtcggg gtttgataaa
    40921 attttaaaaa aaacaatcta gtataaattc acttgttttg gagtactatt tcgttttgta
    40981 atttttttga taaactttta aaaaccaatt cataggaatg gttaatgagt tcccgcatca
    41041 ttttgtcatc cacatcactg tgaaaagcaa ctgtattcca atgaatttta ctcatgtgat
    41101 accctggatt aattgcatca tattcggcac gaagttctaa tgctttttca ggatcacatt
    41161 ttaaatttaa agaaggtgtt ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41281 nnnttttttc gggcatcttc atttaaatta ttcacatctt tcacgttcca aaaggctttg
    41341 tgggccaata atttgccttc atctttttcc ttatctacac ttggtaagtc gattaataaa
    41401 tgttgaatgc ctttttcacg aataaaaata gccgcttctt catgtaaata aggtggatta
    41461 gtatgtgaat aattttttga ctttttttca ttcaaattgg gtaaggtgcg aatcactatc
    41521 gcttccggtt ttgaattacc aagagctttt tctacttgca ttttggtaat gacaaaatcc
    41581 tcaccttgtt tttccggttg aatggaaatc agttgagccg tgaagaaaaa ggttttcaag
    41641 gcctgattga tagagtaaaa ttctttagta atatgtccca aacattccgt gtgcgttcca
    41701 tgtccgtgcg gattaaaaaa aatgttgttg aaattggtgg tggctccacc tttcacactt
    41761 cccacccatg aaccaaaacg aaccggttca atttgcggtt tttcgatgta ccatgcaatc
    41821 ggattttttt ctgtattgga taatggcaac gaaatatcaa tgggttttga taaatcgact
    41881 tggaaagttt tgttttggtg ggttattgtt gctatcaatg tttcaacttt tttaacatta
    41941 agaaattaag ggctatttca ttaagtttca ttaaggagta ataaaacttt tccaaattta
    42001 ataaaataag ttaagactta atgaaaacct taattatcct taacttctta atgttaaaag
    42061 ttatattaag ttcaacataa aaagatcaga agaaatccca tcactcagaa actttccttt
    42121 tcgagtggtt cgcaaaatat tttcatccac atgaagcaaa tgatcttcaa tgtatttagc
    42181 cgcttgttgg ttgagataat ctaaataggt ttgtccaaat tcggattgaa ttctgtcaag
    42241 agaaattccc caaatcgttc gtaatccggt cataacatat tcgttgtacc gatcggtttt
    42301 ggacaatatt tctgtttcaa tgggcaattt gccttcttgt attgatttga tgtacaagga
    42361 attattagat acattccaac cacgtttttc accatcataa ctatgtgcag aaggtccaat
    42421 gccaatgtat ttttttccta accaataact cgaattgttt ttagaaaaaa aattttcctt
    42481 cccaaaattg gacagttcgt aatgaataaa tccggcctct tctaattttt caaccaaaat
    42541 ttcaaagtgt tcttgagcga cttcgtcttc cggtggtggt ataattcctt tttgtataaa
    42601 cgaatgtaaa gccgtttttg gttccaccgt taacgcataa cttgaaatgt gcggaatacc
    42661 aaaagacaaa gcggtttcga tattttgaat ccatttttcg ttgctcatgc ccggaatgcc
    42721 gtaaatcaaa tcgagtgaaa tgttgtcgaa atgtttggtg gcttcttcta aacatatttt
    42781 ggcttcttcg acattatgag cccgattcat cattcttaaa tcatcttcaa aaaaggattg
    42841 aatgccgatg gagagtcggt taattcgatt gttggatagt tcgattattc gattattcga
    42901 taagtcatct ggattcgctt cgactgtaat ctctgggttt tcaatgacat tataattttt
    42961 aaaaacttcc tcaatcaaca atcgtaaatc ttcaatagcc agaacagaag gagttccgcc
    43021 accgaaataa atggtttcga ttacatcatt tttaaattct tcttttcgca tcgagatttc
    43081 tttcactaaa gccaaaacca tctcgtcttt ctttttcatg gaagtagaaa aatgaaagtc
    43141 gcagtaatga ca
//
LOCUS       DAOTCW010000005        38921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_503_length_38921_cov_40.137833, whole genome shotgun sequence.
ACCESSION   DAOTCW010000005 DAOTCW010000000
VERSION     DAOTCW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 38921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38921
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_503_length_38921_cov_40.137833"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="P5335_00825"
     CDS             complement(<1..755)
                     /locus_tag="P5335_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRZ73452.1"
                     /translation="MSQRDKDDLKLAVLIDADNVPFSNVKGMMEEIAKYGTPTTKRIY
                     GDWTKLPDKGWKSVLLENAITPIQQYGYTTGKNATDSAMIIDAMDILYSDKVDGFCIV
                     SSDSDFTRLATRLRESGMKVIGIGEKKTPNPFIVACDRFIFIEVIEGAKKKKVVSATA
                     TADDKKKDEKALSKIDVDTIDIIESSIEDIAEDDGWAFLGDVGNLIVKKKPEFDPRNY
                     GFSKLTPLMKSLTHLLEIHERESDKKGIKHVYVR"
     gene            949..2208
                     /locus_tag="P5335_00830"
     CDS             949..2208
                     /locus_tag="P5335_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HRZ73453.1"
                     /translation="MVKDLFERIQNNKGPLGKWASQAEGYFVFPKLEGELGPHMQFQG
                     KDILNWSLNDYLGLANHPEVRQADIDAAIQYGAAYPMGARMMSGHTNAHEQLENELAA
                     FVKKESAYLLNFGYQGIMSTIDALVTKNDIIVYDVDAHACIIDGVRLHMGKRFTYRHN
                     DLESMEKNLQRATKMAEETGGGILFITEGVFGMRGQQGKLKEIVAFKEKYNFRLLVDD
                     AHGFGTLGKTGAGAGEEQGCQDGIDVYFSTFAKSMASIGAFIAADKDIIDYLKYNLRS
                     QMFAKALPMIQTVGALKRLELLRNHPELKDKLWENVNALQSGLRARNFNIGDTNTCVT
                     PVYLEGSVPEAMVMVNDLRENYGIFLSIVIYPVIPKGIILLRMIPTSSHSLADIDRTL
                     TAFEAIREKLVNGTYKKIAETTTVDVS"
     gene            complement(2302..3420)
                     /locus_tag="P5335_00835"
     CDS             complement(2302..3420)
                     /locus_tag="P5335_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase"
                     /protein_id="HRZ73454.1"
                     /translation="MITIVEAISKKSLTDYVKFPFSLYKNHPYWVPPLIDDELETFDK
                     VKNPAFDNAEAYFYLAYRDNKIVGRISAIINWEEVNNQQKKKVRFGWWDVIDDIEVTK
                     ALLEKVYELGRKNKLEYVEGPMGFSNLDKVGVLTEGFNRMGSMITWYNYPYYKDHLEQ
                     LGYVREKEYFESVFSFSDVKPETFAKASDLIEKRYSLKTVTFTKTKDIIPYVDKMFDL
                     FNTSYAKLASFVAVSDRQKEYFKKKYIPFINPEYILFILDKNDKMVAFSIVMPGFSEA
                     LQKANGRLFPFGFYHLLKAKKHSKEVLFYLIGIEQEYLNKGLTAIIFREYYNVFKEKG
                     IEICNRTPELEENNAIHNLWKNFNSHIDRKRRTYRKDL"
     gene            complement(3425..4390)
                     /locus_tag="P5335_00840"
     CDS             complement(3425..4390)
                     /locus_tag="P5335_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRZ73455.1"
                     /translation="MKNLKIAFLFVFNFSVSFVIAQHTDMINSNRPGQSMSAFSVGKT
                     VFQMETGIYGFKEVHDAARYNSNGMGLDLDFRYGLYFEELEVIVNSKYQYDKYYSPLV
                     DETRSGFRSLVVGAKYLLYDPFKNYEEKPNIYSWKANHRFKWRQFIPAVSVYAGVNIG
                     VANEDYAYPDEATISPKVMIIAQNHFGTSWVLVTNIYYDKFTTDYSSLAYILTLTRGF
                     NEKWSGFIENQGINGDYYSDSILRGGAAYLLKSNLQIDGSISTNFKDTPSILYGGVGI
                     SWRFDEKYEPIKVEIGEGAEKRAGSKKKKDKQKKEAKKRVDEVEG"
     gene            4511..4753
                     /locus_tag="P5335_00845"
     CDS             4511..4753
                     /locus_tag="P5335_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 domain-containing protein"
                     /protein_id="HRZ73456.1"
                     /translation="MQTASFTGLVRTLLILIGIYYAFKIIARILMPIILRKMVQKAEQ
                     NFKQQTQNQPQQTASNFDKQKVKKEVGEYIDYEEVE"
     gene            4785..7214
                     /locus_tag="P5335_00850"
     CDS             4785..7214
                     /locus_tag="P5335_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRZ73457.1"
                     /translation="MKYFQKSLPHLLSLFGFVFISLIYFYPVLQGKKILQSDIVQYTG
                     MAKEQNDFRATTGEEPFWTNAAFGGMPTYQLGAQYPHNYVKKLDGALRFLPRPADYLF
                     LYFLGFYVLLSVLKVDPLKAFFGALAFGLSTYLIIILGVGHNAKAHAIAYMPMVIAGV
                     ILVFQKKFIVGGLLTMIAAALEIQANHFQMTYYLLLLLLILSGYYIFQFIKEKDFKSI
                     GIAFGVFLFAGILALGVNATNLMATAEYTEFSTRGKNELTIGPEGEKIENKSGMTYEY
                     ITEYSYGIAESLNLIAPRLFGGASNEKLDETSNLYEFFVGMGAPENEALDYANGSRVY
                     WGDQTSVAAPAYIGAIVFFLFVLGMFTEKSKLKYAFLAGAIFSLMLSWGKNFPIMTDF
                     FIDYFPLYNKFRAVSSIQVILELCIPVLAIMGLYSFFKLEKDQQWQALWKSAAISLGI
                     LALLILVKGMFSFSTESDAYFKQAYGEIGQPFVDALKEDRRAMYTSDLLRSMGLILTV
                     SAVFWLFIKNKLSQTIAVVLAGLLMVLDLYVIDKNYVNADDFVNARQVDEPFQPTPAD
                     LEILKDTDPNFKVFEPGQGMAGGRTSYFHKAIGGYSAVKPQRIQQLYDYQIANNNIQV
                     LNMLNVKYVIQTTEEGQPIPLRNPDANGNAWFVENIMIVDSADKEIKALDSLDTKKIA
                     VVHKEFYSNSKNNAFIKDSLATIKLEEYKPNYLKYTAQNANDGVAIFSEIYYPNGWKV
                     TIDGKEVKEFRANFVLRGLLIPAGKHTIEFKFEPEVVKKGSSIALFSSIGMLMLLLSG
                     LYIESKKKKVD"
     gene            7255..8538
                     /locus_tag="P5335_00855"
     CDS             7255..8538
                     /locus_tag="P5335_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRZ73458.1"
                     /translation="MKKILIITYYWPPAGGPGVQRWLKFVKYLPDFGYEPHVYIPENP
                     TYPIIDEKLIAEVSSKAIIIKKKIREPYRWASFFSKKDTQKISSGIIPSKKKQSLVAK
                     FLLWIRGNLFIPDARIFWVKPSVEFLENYIQEHQIETIITSGPPHSLHLIGLELKEQL
                     NVKWIADFRDPWTTIGYHKELMLTEKSEKKHKILEKKVLCSATKISVTSPSTKIDFEK
                     ITNRPIAVITNGYDVEDVSKTLLDEKFTLAHIGSFLSERNPRILWKALKELVKEEPGF
                     ASDFRLKLIGKISQDVLDSIAEFKLSKYVDNLGYVSHQEAIMHQRKSQVLLLIEIDSE
                     ETRCIIPGKLFEYMVSERPIIAIGPKASDFEMILKETNTGTFFGYDEKKRLKTTILAH
                     YSLYKESNLKVYPVGLQHYSRKNLTQQLVNFINEA"
     gene            8543..10018
                     /locus_tag="P5335_00860"
     CDS             8543..10018
                     /locus_tag="P5335_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRZ73459.1"
                     /translation="MGIVLNQSVKNTVITFIGFAIGAANALFMYTHFLGEDYYGLTAF
                     LLSSANIMMPLMAFGIQNTLVRFYAEHQTEEDKSRFLNLVLVLPLLIIIPVFLILFFF
                     YPETASKLSVENPIIYDYVWIIPVIGLFMGYFEIFYAWVKVHLKSVFGNFLKEVLLRI
                     FISIFLFAVYFGWITSIQFVYSLVFIYLTMMLIMAFVAFKIRKPQFHLKFPKEKKEVI
                     IYSAFIIFSSSIAVMLLDIDKFMIGQYIPLKEAAYYSVAIFIALTISVPMRAMHQITH
                     PVTTDLMARKKHTELNDLYKKASITLQVIGGLIMLGILTNIHQVYALLPEKYSGGVAV
                     VFIISISKFFDLMLGNNNSIIFNSKYYRTVLLLGVCLVVLTVSLNMYFIPNFGIEGAA
                     LATLLSIGLYSLAKLLFVVIKMDLFPFTRNTLLSLGIIGLTFMVFYFWNFPFHPIINI
                     ILKSVLLSFFYLVLNYKLKISVDINDVIEKILKRIYIMRIE"
     gene            complement(10135..10767)
                     /locus_tag="P5335_00865"
     CDS             complement(10135..10767)
                     /locus_tag="P5335_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73460.1"
                     /translation="MKKITLLVAVNLLVGSLAFASETIVDTDTDRRHNVAVHYMDAEP
                     IVFMEREIEFYVFLNGDFDFNTRPQDSQGGYYYKRGATARGNSAVGNFGVRIEHDSFG
                     RVRRIGNVFINYDSQNRVNRIGTVYMRYNRFALAQIGGMELVYNRKGHLVDMFGEVKM
                     RARAYGYATTTYYGSSNYYTNTDYAYNNDPYYYYKADGTKALIEDKKDKK"
     gene            complement(11251..>11783)
                     /locus_tag="P5335_00870"
     CDS             complement(11251..>11783)
                     /locus_tag="P5335_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73461.1"
                     /translation="ETVSYSNTDNPNIFLYFYNTDGKRTAKLVDTEQMQNHFFSVSRQ
                     VTTNVEVLHFKYINSEKKEENKLFRQYPTYYDFKTTKRDSLYKTVVLQFYTNEKKKKV
                     NLRLELKIKEADANYFPNYRIATLHGASERKELNYTENGLVVEAFDLTKKKKKEQAKL
                     IYSEKIQFELVVPTKK"
     assembly_gap    11784..11883
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12061..14853)
                     /gene="uvrA"
                     /locus_tag="P5335_00875"
     CDS             complement(12061..14853)
                     /gene="uvrA"
                     /locus_tag="P5335_00875"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRZ73462.1"
                     /translation="MTPEEFALLEPKKNILIKGAQLHNLKNIDVAIPRNQLVVITGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSSYARQFLGRLDKPKVEYIKGIAPAIAIEQKVNT
                     TNARSTVGTSTEIYDYLKLLFARIGKTFSPISGNEVKKHTVTDVVKTISTFEPDSKWL
                     LLAPIHLETGRQLEDKLKVLLQQGFIRVLVDNETVRLDELEQHKLDNKDILLIIDRIV
                     VKSDEEFLNRLADAVQTAFYEGKGDCFLQEVLTGNRIGFSTNFQLDGLTFLEPNIHLF
                     SFNNPYGACPSCEGYGNVIGIDEELVIPNTALSVYENAIFPWRGESMGWYRDQLVNNA
                     YKFDFPIHKPFYQLSEDQKQLIWNGNSYFTGLNDFFHELEEKNYKIQNRVMLSRYRGK
                     TKCSTCKGKRLRKEANYIKVGNKTISDLVDLPIKNLISYFTELQLSEYDLKVAKRLLV
                     EINSRLSFLQKVGLDYLTLNRNSASLSGGESQRINLATSLGSSLVGSMYILDEPSIGL
                     HPKDTERLIDVLKNLRNLGNTVIVVEHDEDIMKAADRIIDIGPEAGTLGGYLVAEGNF
                     NEILKADSLTAKYLNGELEIEIPKKRRKFKNHIEVIGARENNLKNIDVTFPLDCLTVI
                     TGVSGSGKSTLVKKILFPALQKKLEGVSDKAGQFTELRGNFSHIKHIEYVDQNPIGRS
                     SRSNPVTYIKAYDDIRDLFTKQKLSKIRGYLPKHFSFNVDGGRCETCKGDGEVTIEMQ
                     FMADVHLECETCGGKRFKKEILEVQFEGKNIDNILTMTIDDALAFFKEQNQAKIVQKL
                     QPLQDVGLGYVQLGQSSSTLSGGEAQRIKLASFLVKGTTKDKALFVFDEPTTGLHFHD
                     IKKLLASFEALIEKGHSIIVIEHNLDLIKCADYIIDIGPEGGENGGKLMAFGSPEDVT
                     KNPKSITGKYLKEKLR"
     gene            15126..15710
                     /locus_tag="P5335_00880"
     CDS             15126..15710
                     /locus_tag="P5335_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRZ73463.1"
                     /translation="MATVNLPDALLVKNYIAGDEKALAILIERHQSKIYGFIYSKIAD
                     RDITDDVFQDTFIKVIKTLKSNSYNEEGKFLPWVMRIAHNLVVDHFRRNKKMPMHRET
                     EEYSIFSYMTDNSMNVEGRMITDQVEDDLRRLLDELPADQKEVLVMRMYQDMSFKEIA
                     ESTGVSINTALGRMRYALMNLRKVIEKNQIILTN"
     assembly_gap    15957..16056
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16134..16586
                     /gene="bcp"
                     /locus_tag="P5335_00885"
     CDS             16134..16586
                     /gene="bcp"
                     /locus_tag="P5335_00885"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRZ73464.1"
                     /translation="MTTLKKGNQAPAFSGKDQDGSVHSLSDYKGKKLVVFFYPKANTP
                     GCTAEACDLRDNYARFEAGNYTLLGVSADNAKAQGNFKNKYEFPFPLIADEDKSVIQA
                     FGVWGPKKFMGKEYDGIHRTTFVIDENGIIEEVISEVKTKAHANQILG"
     gene            complement(16640..18097)
                     /locus_tag="P5335_00890"
     CDS             complement(16640..18097)
                     /locus_tag="P5335_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ73465.1"
                     /translation="MEIKLKGDKAIEQIPSIKDKALRINLNENIYGTFAEIGAGQETV
                     RHFFRSGGSSGTIAKAMSAYDKDFSDSIYGVEKDGRYVTESRLKKMLHHEVNLIEKRL
                     DRVKHPNKMFFSYANTVATIDFAKQFKGHGWVGIKYQIEPDEDYNEIILHIRFKETDA
                     RLQQETLGILGVNLIYGAFYKYNDPKKLLRYLYDHLDKDQLEIDTINFSGPRFANVDN
                     RLMSLQLVKNGMTDAVMFGPDGTNILPASILYKKNILALRGSFRPVTSVNMDMYEKSL
                     EMFLNESKVDADNTLVIFEITLSNLRSEGEIDERDFMDRAELLCSLGQYVMISNFQEY
                     YRVVEYFSSYTKARMGLAMGVNNLVDIFDEKYYRHLSGGILEAFGKLFYRDLKVFLYP
                     MKSEDGTIMNSENLKVHPRMKELYKFFKFNGKVVDIEDFNHNNLEIYSRHVLKMIAKG
                     TPGWEEMLPKGVAEIIKRDNLFGYDPKKIIVEQTK"
     gene            18199..18963
                     /locus_tag="P5335_00895"
     CDS             18199..18963
                     /locus_tag="P5335_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRZ73466.1"
                     /translation="MKVYFLGTGTSQGIPVIGSHHSVCKSTDVKDKRLRVSVWIHDEK
                     SSIVIDCGPDFRQQMLTCRCQKIDAILFTHEHADHTAGLDDIRPFFFKQGDIPIYAHK
                     RVIANLKKRFDYIFETENKYPGAPSVAIHEVVKNNTFKIGQKNVIPIQVMHGSLQVFG
                     YRIDSFAYLTDVKTIESSELEKLKNLDVLVVNALREEPHETHFNLQEALDFITLVNPK
                     RAYLTHISHLLGFHEEVQQKLPSNVYLAYDNLEIII"
     gene            18966..19451
                     /locus_tag="P5335_00900"
     CDS             18966..19451
                     /locus_tag="P5335_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73467.1"
                     /translation="MKKSLFLYLFIVSFLMMIFTYMYYSKKVKFEAERFEKLKVKMND
                     SIQSLNNQVFEANYFTLLNNDNAQNYLEDYSIPKLISKIEQNLMELNDNPEGNPLVEY
                     GKIGDQKFIINKIKVINHRWIIADFSNGDLWGEVLLQYFIEEDEQLTFKIIQSTLYVK
                     K"
     gene            19490..19981
                     /locus_tag="P5335_00905"
     CDS             19490..19981
                     /locus_tag="P5335_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73468.1"
                     /translation="MKNSLLLLFVVFNLVSCKKFDQEIEPEIAKKQTFIDTVENSDKD
                     ENGCLTSAGYVWSQLNKECVKIYQSAIILYPHANQTNEDETLNAYVVFSKEGGNEAEI
                     FFPNTEKSHLFIRETEGQPWKYQDWQLIPWKGFILKKGEEIKFSGDGEFGPKVTGSDK
                     IEN"
     gene            complement(19983..>20135)
                     /locus_tag="P5335_00910"
     CDS             complement(19983..>20135)
                     /locus_tag="P5335_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HRZ73469.1"
                     /translation="DQVIPVEWARKTPQLLENFGIDVVYKEYPIGHGVSPQNFFDFRK
                     WLLENK"
     assembly_gap    20136..20235
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20236..20489)
                     /locus_tag="P5335_00915"
     CDS             complement(<20236..20489)
                     /locus_tag="P5335_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HRZ73470.1"
                     /translation="MSLHYLVREPKIIQEKNPLLLLLHGYGSNEADLFSFASELPEEY
                     FVISAQAPYDMQYGSFAWYAINFDADENKFSDLNQARQSR"
     gene            complement(20492..20803)
                     /locus_tag="P5335_00920"
     CDS             complement(20492..20803)
                     /locus_tag="P5335_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73471.1"
                     /translation="MEQNKNAAIVAYLTIIGAVIAIFMNSESKSTFASFHIRQALGIF
                     ITFFALGYVIGYFDDWMVSSAFYLFFFILWIYGFMGALNGQQKSIPLLGGFYQKLFKN
                     L"
     gene            complement(20805..22058)
                     /locus_tag="P5335_00925"
     CDS             complement(20805..22058)
                     /locus_tag="P5335_00925"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRZ73472.1"
                     /translation="MNLIIRNATIIDQNSPYHNQTIDLKISGSSIIKIGKNLEKTSDF
                     EEISFENLHVSPGWFDTSVSLGEPGFEERETIANGLSVAAKSGFTAIALQPNTSPIID
                     NQAQIRFVKEKAKENAVDLIPIGAFTKNAEGLDLAELFDMKNAGAIAFGDYGKNISNA
                     NLLKIGLQYVQDFDGLIIAFSQDEKIKGSGVVHEGIVSTRLGLKGIPTLAEELMVARN
                     LFLLEYTGGKMHIPTISTSKSVDLIREAKAKGLNITSSVAIHHLVLTDEKLDSFDTRF
                     KVSPPLRDDFNRTALIQGVLDGTIDMITCDHNPIDIEHKKMEFDMAKNGTIGLENAFG
                     ALQTVLPTEVIVTKLTAGKAVFGLNSNSIKEGEVANLSFFNPNGNWIFAKENVLSKSK
                     NTAFLGQEMKGKVYGIYNNGKLVIR"
     gene            complement(22055..23995)
                     /locus_tag="P5335_00930"
     CDS             complement(22055..23995)
                     /locus_tag="P5335_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HRZ73473.1"
                     /translation="MQFKHPEILYFLFLLVLPILVHLFQLRRFKKEYFTNVKFLKELS
                     IQTRKSSQLKKWLLLLTRLFLLTALIFAFAQPFFKAKDSKGVNNELYIILDNSFSMQA
                     KGKQGELLKRAVQELLENIPENKTFSLLTNDAIYWNTDIKSIQKELMNLNYSVNSFQL
                     DQSLTKIKSRKTPYNKDLVIITDAINLQENQLKPLDKTYNPFFIIPNSEQKNNVSVDS
                     VYLHQTLDNFYEIGVKIKSYGKSDIEIPVALYNQKQFIAKTQIKVNQLEKDLFFTIPK
                     ADFNGYVYLDDSGLVYDNYYYFSISKPEKTNVLAIGQVEKNQFLTKIYTENEFIFSQF
                     ELKSLDYNLLEKQDAVILNELKEIPQALQTTLKSFYEKGGNLIFIPSSETSISNSNFF
                     LRNFGAISMNNSIQKEKLITGISFAHPLYSGVFEKKVDNFQYPKTVSGFELISTYPAI
                     LSYEDQSPFLTAVSTALSSVYVFSAPIHKDNSNFKNSPLIVPTFYNMALQSVKTGINA
                     LPIGTSTPVTVDQLLSKDEIISVISATDEKAEKFIPQQQIVNNKVKLSFVESPTIAGN
                     YNAFKDNSILKNISFNYPRTESDLSLDNSNLVEKYENGNSMETVFNTLQTNRIDNAVW
                     KWFVWLTLLFLVLEVLIQKFVK"
     gene            complement(24102..25445)
                     /locus_tag="P5335_00935"
     CDS             complement(24102..25445)
                     /locus_tag="P5335_00935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006264367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRZ73474.1"
                     /translation="MSTFLDFDLPKSLQKALDEMGFSTPTPIQIKSMPVILSGRDMMG
                     IAQTGTGKTFAYLLPILKQWKFQNIHTPRVLILVPTRELVVQVAEEVVKLTKHMSVRT
                     LGVYGGVNINTQKTAVYQGVDILVGTPGRVMDLALDNVIRFDEIQKLIIDEFDEMLNL
                     GFRFQVTSVLTMLKSKRQNILFSATMTDEVDDMLDEYFDFPIEVSLAPSGTPLEQISQ
                     LGYKVPNFLTKINLLKELLHTDESMSRLLIFINNKKTADLVASSLEEDFPDQFGLIHS
                     NKSQNYRLNTMASFQAGEIRGIVTTDVMARGLDISDITHVVNMEFPEVPEQYVHRIGR
                     TGRADKTGTALSFVSPREEEIQIEAEILMEKEIRFLALPNIEIEEKILEFEKEKRKMK
                     VLLKGPKKEGGEAFHDKKDKNKKVNLGGPGKRTPRKTESRNRGVEKKRDAKRKKK"
     assembly_gap    25508..25607
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(25695..26327)
                     /locus_tag="P5335_00940"
     CDS             complement(25695..26327)
                     /locus_tag="P5335_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HRZ73475.1"
                     /translation="MKFTLAESVTRELFPKNKWLIGYIMLFFMLWVSTFIGTSDYNNW
                     WIENTLTILSLLFLSFSYKKYPFSDLSYFFIFIFLCLHVYGSKYTYAENPLGYWLQDI
                     FNTERNHYDRIVHFGFGFLLTYPLQELFRHWLKLPKTISWMLPIELSISIGALYEILE
                     WAVADIFFKAQGHAYLGTQGDVWDAQKDMFLAFVGAILATIIIRLFKKLN"
     gene            complement(26459..27223)
                     /locus_tag="P5335_00945"
     CDS             complement(26459..27223)
                     /locus_tag="P5335_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HRZ73476.1"
                     /translation="MTKKELKEFLDEKVVLYNHPNFIESDPIQIPHRYQLKEDVEISG
                     FLAATISWGNRKMIIKNGLKLMYLLGNSPYDFIMNHTDNQLETVNNFVHRTFNGIDCI
                     TFIKGLKNIYENHSGLEAVFAKNQQNDSLQLSISEFKKTFFEIEHLNRTQKHISDPMN
                     NSAAKRINMYLRWMARNDKTGVDLGIWKSIPPSKLSCPLDVHSGNVARKLGLLHRKQN
                     DGKALFELDTRLRILDSKDPVKYDFALFGLGVFEGF"
     gene            complement(27227..27934)
                     /locus_tag="P5335_00950"
     CDS             complement(27227..27934)
                     /locus_tag="P5335_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRZ73477.1"
                     /translation="MIQAKNIHKKFDQLHVLKGVDLVVNKGEIVSIVGASGAGKTTLL
                     QIIGTLEKPTPQAGTELIIDEQSVLEMNDKELSKFRNLKLGFIFQFHQLLPEFTALEN
                     VCIPAFIAGKSKTEAETEAKKLLEYLGLSHRMDHKPGELSGGEQQRVAVARALINKPA
                     VIFADEPSGNLDTASAENLHELFFKLRNEFGQTFVIVTHNETLANMADRKLVMVDGLF
                     EKNAGWIDLTNLNPIKG"
     gene            28080..28733
                     /gene="msrA"
                     /locus_tag="P5335_00955"
     CDS             28080..28733
                     /gene="msrA"
                     /locus_tag="P5335_00955"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRZ73478.1"
                     /translation="MKKLFFMMIAFATISCQSQDKKSNYSQVKEPIKIKMTNEMEIAT
                     FAGGCFWCTEAIFLELKGVEKVLPGYIGGTVQNPTYKEVCNGTTGHAEAIQITFNPKI
                     ISYGELLEIFFATHDPTSLNRQGNDEGTQYRSEVFYYTDNQKQLTQDYIALMTTEKTF
                     KNPIVTRVSPASEFFIAEDYHQNYYNLNKTQSYCSYVITPKIEKLKKNYKQKLKSNF"
     gene            28781..29452
                     /gene="folE"
                     /locus_tag="P5335_00960"
     CDS             28781..29452
                     /gene="folE"
                     /locus_tag="P5335_00960"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRZ73479.1"
                     /translation="MNTLIEKELFEILGDEHHMTSAETPLRPDAFDKSDVEKMATIEK
                     HFHIIMEEMGLDMTDDSLRGTPHRVAKMFIQEIFSGLNPANKPKISVFENSYNYDKML
                     VEANISFNSTCEHHFLPIIGKAHIGYVSNGNVIGLSKLNRIVDYYARRPQVQERLIMQ
                     IFNELKSVLNTEDVIVVMEAEHLCVSSRGIKDSSSFTSTIQYGGIFNDKENRNDFFNL
                     LKQDK"
     gene            29500..29793
                     /locus_tag="P5335_00965"
     CDS             29500..29793
                     /locus_tag="P5335_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73480.1"
                     /translation="MTKEILVKPVKDTKKRDLVLGLLFDAIGFISYAVPFLGELTDLV
                     WAPIAGLILLKMYKGTVGTVGGITVFIEELIPGLDFIPTFTLTWIYTYWIKKK"
     gene            complement(29844..30167)
                     /locus_tag="P5335_00970"
     CDS             complement(29844..30167)
                     /locus_tag="P5335_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglyceryl transferase"
                     /protein_id="HRZ73481.1"
                     /translation="MEKLKKRWGITSNFQLIIIFIVFAITGSLSAYLSKPFTNYIGLT
                     RENLGGFILPIRLLAIFPIYQVLLVFVGFIFGQFKFFWWFEKKMLRGMKLGFIADLFE
                     KRKIE"
     gene            complement(30171..30599)
                     /locus_tag="P5335_00975"
     CDS             complement(30171..30599)
                     /locus_tag="P5335_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6146 family protein"
                     /protein_id="HRZ73482.1"
                     /translation="MKKWFILIALNYIICSCSGSKNGMASTENNPNVNDTIRIANDEL
                     EYEIIIIEPGFNSWLLATAKPRNYYDQSYLENRNRIWVVNYNQRVFDRNRYDPMLYEM
                     QIDYQNNINYGYEVNYILYNYFVYFQQKYKQKLGGFEPRL"
     gene            complement(30702..31661)
                     /locus_tag="P5335_00980"
     CDS             complement(30702..31661)
                     /locus_tag="P5335_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRZ73483.1"
                     /translation="MKVLANDGISKSGIKALEKGGFEVITTKVAQEQVANFINTHQIR
                     VILVRSATKVRKDIIDACPSLKIIGRGGVGMDNIDVAYAREKGIHVINTPASSSESVA
                     ELVFAHLFSGVRFLHDANRNMPLEGDTNFDGLKKAYANGIELRGKTLGIIGFGRIGQA
                     VAKMALGLGMKVIASDKYVGEAVIRVDFYNGQFINVDIQTEPMEDLFKHADFITLHVP
                     AQEGYVIGRDELKGMKDNVGIINAARGGVIDEVALLEALDEGKVLFAGLDVFENEPTP
                     EIKILMHPKISLTPHIGAATEEAQDRIGTELAEQIISLLKTEK"
     gene            complement(31755..32825)
                     /gene="serC"
                     /locus_tag="P5335_00985"
     CDS             complement(31755..32825)
                     /gene="serC"
                     /locus_tag="P5335_00985"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRZ73484.1"
                     /translation="MKKHNYSAGPCILPQEVFEKSAQAILNFNNSGLSILEISHRSKD
                     FVAVMDEARALALELLGLQGKGYQALFLHGGASLEFLMVPYNLMKVNGKAAYLDTGTW
                     ANSAIKEAKSFGETVVVASSKAENYNHIPKGYHVPSDADYFHCTSNNTIFGTQIKEFP
                     TLDIPMVCDMSSDIFSRTLDFSKFDLIYAGAQKNMGPAGTTLVVVKEEILGKSGRNIP
                     NMLDYKQHIDKESMYNTPAVFPVYASLLTLQWLKNLGGISAIEKVNEAKANLLYTEID
                     RNPLFKGTAEPEFRSNMNATFLLNDEAHAPLFDSMWKAAGISGLPGHRSVGGYRASMY
                     NALPLESVQVLVDVMKELEKRS"
     gene            complement(32950..34359)
                     /locus_tag="P5335_00990"
     CDS             complement(32950..34359)
                     /locus_tag="P5335_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ73485.1"
                     /translation="MKTKTILISFYFVSNFLFAQTNLSGFYPSSTISGLRFGSASAIH
                     ENTIVVSSATNQFPPLTTGYVYVFNQTNQSITQEAYFYPSDALSNDLFGKSVAVFGDF
                     IAVGSPLHDEGFSDSGAVYLYKKVNNNWEFFQKITASDASANKQFGSFVKIQGNSLFV
                     SAPNDANSIGIGSVYVYTFNETEWIFNQKLTIANSIKLGEKIEIEGNYLVVSDRLNEQ
                     NTKYHTFIFETNWIYNDSTLEFGNLEENIKDFSFDGQKLYITAIQMSSQFLNKVYILN
                     RVNNSWVNESILPMNFDDFVIGKIAVSGNNMLVGYEFYFLQLSRKFPVFYYKKIEYEW
                     QMQTYFYGEGQSNMDDFLGNSISLQGSNLILGANREGIINQGKAYSYNLESLSIPTFN
                     KNVISIYPNPTNNSITIENQTSNQVERYELFSITGKLLLSVSNQISTLSLDHFQSGIY
                     LFKISFENGSYETQKIIKK"
     gene            complement(34395..35453)
                     /locus_tag="P5335_00995"
     CDS             complement(34395..35453)
                     /locus_tag="P5335_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HRZ73486.1"
                     /translation="MLQKTKINSFVALGDFLSQFSLDSNLRKQGVLNNDAFFDSFEAI
                     LELSQSHNGWFTKEQVYFSVNSWVEALTETNLNEWLLNYDFSKVTPKKVGLILAGNIP
                     LVGFHDFLSVLITGHTVLIKTSSNDQHLIKFFANYLISVDKDLEERITFTDGKLENFD
                     AVIATGSNNTARYFEYYFKNKPSIIRKNRNSVAILTGNETQEDLVNLGEDIFRYFGLG
                     CRNVSKIFVPNGYNFDSFFKAIFEYQDVIHYEKYANNYDYNKAVFLMSNFKLLDNGFL
                     TLKEDSSYSSPISSVFYEFYEDLEILKNRLKNEGDQIQCIVSNHLIDNSIAFGQTQKP
                     KLWDYADNVDTLQFLISV"
     gene            35517..35867
                     /locus_tag="P5335_01000"
     CDS             35517..35867
                     /locus_tag="P5335_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRZ73487.1"
                     /translation="MAIIITDECINCGACEPECPNTAIYEGADDWRYKDGTKLKGKVI
                     LPDGTEVDADAAQTPISDDIYYIVPGKCTECKGFHDEPQCAAVCPVDCCVPDDNHVES
                     EETLLNRQSFLHNE"
     gene            35902..36930
                     /locus_tag="P5335_01005"
     CDS             35902..36930
                     /locus_tag="P5335_01005"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRZ73488.1"
                     /translation="MKKIFLVLFFLFHFNFLFSQSIKGKVVDENNSPLYGVSVYFDGT
                     TIGTTTNNEGVFEFSVNQIPNVNFVISYIGYETVYMLSIQSPINIKLNPLEISLKEVV
                     LEPIPFSRKEMLTVFREHFLGRTKGGKNSVILNEEAIQFSYTAKEFELAAFADEKIRV
                     RNNFLGYIIEVDLIDFYILYNKKTLSNNFLSGSYFAVTSFYKEINGVTKSFEKNRISS
                     YLGSSKHFFKNLIDKKWGKKEFVLYDGSFATDADFHFEILDAKDMKLIRVKEKQITTN
                     LLSTQKFFRSFNMLYNNKEQSGIIFKTNEFYVDIFGNHSNIDQIDFSGEISKKRFGDM
                     LPLDFVLK"
     gene            complement(36974..37510)
                     /locus_tag="P5335_01010"
     CDS             complement(36974..37510)
                     /locus_tag="P5335_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73489.1"
                     /translation="MKLVAIIVLIMTFSFGYSQNKNIKEETKTTITTVDNGQGEKQTI
                     KNENIKEVQLLEHQAPTSNTINVEQKDSPIIATKTTEIINPDGTIRTVDIDRSSYYLL
                     DGVKYKVSSEPNGYSILNETNVATGNIYKTSTNSYIYSSKNKTSIGFFDSIGNLVLET
                     YNPKTDKVTFETYILVKQ"
     gene            37725..38819
                     /gene="ychF"
                     /locus_tag="P5335_01015"
     CDS             37725..38819
                     /gene="ychF"
                     /locus_tag="P5335_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRZ73490.1"
                     /translation="MKAGIVGLPNVGKSTLFNCLSNAKAQSANFPFCTIEPNIGVVNV
                     PDPRITRLEELVKPERVQMATVDIVDIAGLVKGASKGEGLGNQFLGNIRECNAIIHVL
                     RCFDNDNIVHVDGNVNPIRDKETIDIELQLKDLETVEKRLEKTNRAAKTGNKEAIVEK
                     ALLDRIREALLQAKSARTVIPQNQDEIDLMEEFQLITTKPVLYVCNVDEASAVNGNKY
                     VDQVRELVKDEQAEVIVLSVGAEADITELESYEERQVFLEDMGLTEPGSAVLIRAAYK
                     LLKLQTYFTAGVKEVRAWTINVGDTAPKAAGVIHTDFEKGFIRAEVIAFEDFSNYGSE
                     AKVKEAGKLRVEGKEYIVKDGDVMHFRFNV"
BASE COUNT        12774 a         6102 c         6198 g        13447 t
ORIGIN      
        1 cgaacataaa catgtttaat tccttttttg tctgattcgc gttcatgtat ttccaataaa
       61 tgagtcaacg atttcatgag tggagttagt tttgaaaaac cgtagtttcg aggatcaaat
      121 tcgggtttct tttttacgat aaggttgcct acgtcaccta agaaagccca accatcatct
      181 tctgcaatgt cttcaatact ggattcgatg atgtcaatgg tgtccacatc aattttagat
      241 aatgcttttt cgtctttttt cttatcgtca gcagtagcgg tagcagatac tacttttttc
      301 tttttggcac cttcaataac ttcaataaaa atgaaacggt cacacgcaac aataaatggg
      361 ttaggtgttt tcttctcgcc gatgccaatt actttcatac cagattctct aagtcgagtg
      421 gctaatctgg taaaatcact atcgctggaa acgatgcaaa aaccatcaac tttatcagaa
      481 tatagaatat ccatggcatc gataatcata gcagaatcgg ttgcattttt tccggtggtg
      541 tacccatatt gctgaattgg agtgatagca ttttcgagca aaacactttt ccagcctttg
      601 tcgggtagtt ttgtccaatc accataaatt ctttttgtgg taggggttcc gtattttgca
      661 atttcttcca tcattccctt aacattgctg aagggaacat tgtcagcatc aattaaaacg
      721 gccagtttta aatcatcttt atctcgttgg ctcatgaata ggtctatttt tctagttcaa
      781 atatagttaa aatacctcat tttaaccgca tattggttgt aagttgagaa gtaaaacact
      841 ccgttttaca aatcttgtaa tttcgcaact aaaaatagta tgaaataatt atagaaatgt
      901 ttacttttgt cgcgttttta cacaccaaat taaatatatt aatttatcat ggttaaagat
      961 ttattcgaga gaattcaaaa caataaaggt ccgttaggaa aatgggcttc gcaagcagaa
     1021 ggttattttg ttttcccaaa attagaaggc gaattgggac ctcatatgca gtttcaagga
     1081 aaagatattt taaactggag tttgaatgat tatttagggt tagctaatca cccggaagtt
     1141 cgtcaagcag atattgatgc agcaattcaa tatggtgcag cctatccgat gggagctcga
     1201 atgatgagcg gtcacacgaa tgcacacgag caattagaga atgaattagc tgcttttgtt
     1261 aagaaagaat cggcttattt gttgaacttc ggataccaag gaatcatgtc taccattgat
     1321 gcattggtta ctaaaaatga cattatcgtt tatgacgttg atgcacacgc ttgtattatt
     1381 gatggtgttc gtttacatat gggaaaacga tttacgtatc gtcacaacga tttggaaagt
     1441 atggagaaaa accttcaacg tgctactaaa atggctgaag aaacaggtgg aggaattcta
     1501 tttattaccg aaggagtatt tggaatgcgt ggtcaacaag gaaaattgaa agaaattgtt
     1561 gctttcaaag aaaaatacaa tttccgtttg ttagttgacg atgcacatgg ttttggtaca
     1621 ctgggaaaaa caggagccgg agcaggagag gagcaaggtt gtcaggatgg aattgatgtg
     1681 tacttctcta cgtttgcaaa atccatggca agtatcggag cattcattgc tgcagataaa
     1741 gatattattg attatttaaa atacaattta cgttcgcaaa tgtttgccaa agcattacca
     1801 atgattcaaa ctgttggtgc tttaaaacgg ttggaattgt tgcgtaatca cccggaatta
     1861 aaagataaat tgtgggaaaa cgtgaatgca ttgcaatcag gtttacgagc cagaaacttc
     1921 aatattggtg acacaaatac ttgtgtaacg ccggtttatt tagaaggaag tgtaccggaa
     1981 gcaatggtta tggtgaatga tttaagagaa aactacggta tttttctttc tatcgttatt
     2041 tatcccgtta taccaaaagg aataattttg ttgagaatga ttccaacgtc atctcattca
     2101 ttagcagata ttgatagaac attaactgct tttgaggcta ttcgcgaaaa attagtgaat
     2161 ggaacgtata aaaaaattgc agagactact acagttgatg tttcttaatg taaaagctgt
     2221 aagcttttag cttttaacac gaactagaaa atatattagt atatttatag aaccacttct
     2281 aaaaaggaag tggttttgtt tttataaatc ttttcggtaa gttcttcttt ttcggtctat
     2341 gtgcgaattg aagtttttcc aaagattatg aatggcatta ttttcttcta attcaggagt
     2401 tcgattacaa atttctattc ctttttcttt aaaaacattg tagtattccc tgaaaataat
     2461 tgcggtaagt cctttgttta gatattcctg ttcaattccg attaaataga ataaaacttc
     2521 tttactgtgt tttttggctt ttaataaatg ataaaaccca aaagggaaca aacgtccatt
     2581 tgctttttgc aaggcttctg agaaacccgg catgacaata ctgaaagcaa ccattttatc
     2641 atttttatct aaaataaaca gaatatattc aggatttata aatgggatgt atttcttttt
     2701 aaaatactct ttttgtctgt cagaaactgc cacaaaggag gctagcttcg catagctggt
     2761 attgaataaa tcaaacattt tatccacata gggaataata tcttttgttt tagtaaacgt
     2821 aactgttttc agactatatc ttttttcaat caaatcactt gcttttgcaa aagtttccgg
     2881 ctttacatct gaaaaagaaa aaacactttc aaaatactct ttttcccgaa cgtagccaag
     2941 ttgctctaaa tggtctttgt aatagggata attataccaa gttatcatag accccattcg
     3001 attgaaccct tcggttaaaa cgcccacttt atcaagattt gaaaaaccca ttggaccttc
     3061 tacatattcc aacttgtttt ttctaccgag ttcatacact ttttccaaga gagctttggt
     3121 tacttcgatg tcgtctatta catcccacca accaaatcga actttctttt tttgctggtt
     3181 atttacttct tcccaattga taattgccga gattctacca acaattttat tgtcgcgata
     3241 agccaaataa aaataggctt cggcattatc aaaagccgga tttttcactt tgtcaaaagt
     3301 ttctaattcg tcatcaatta agggaggaac ccaataggga tgatttttat ataaagaaaa
     3361 cggaaattta acgtaatcgg ttaaactctt ttttgaaatg gcttcaacta tagttatcat
     3421 atgcttatcc ttcaacttcg tccactcgtt ttttagcttc tttcttttgt ttgtcttttt
     3481 tctttttcga accggctctt ttttcggctc cttcacctat ttccactttt atcggttcat
     3541 atttttcatc aaatcgccag gatataccca cgccaccata aagaattgat ggagtatctt
     3601 taaaattagt actgattgag ccgtcaattt gtaaattaga tttaagtaaa taggccgcac
     3661 cacctctaag aattgaatcg gaataataat caccattaat tccttggttt tcaataaaac
     3721 cggaccattt ttcattaaat cctcgtgtta aagttaagat gtaagccaat gaactataat
     3781 cagtagtgaa tttatcataa taaatattag taactaaaac ccaactggta ccaaaatgat
     3841 tttgagcaat aatcatcact ttcggactta ttgttgcttc atcaggatag gcataatctt
     3901 cgtttgcaac cccaatattt acaccagcgt aaacagaaac tgcaggaata aattgtctcc
     3961 atttaaaccg atgattggct ttccaactgt agatattagg tttttcttca taatttttaa
     4021 acggatcgta gagcaggtat tttgctccca caactagcga acgaaatcca ctacgggttt
     4081 catccacaag tggagaataa tatttgtcat attggtattt tgaatttaca atcacttcta
     4141 attcttcaaa atataaacca tatctaaaat ctaagtctaa acccattcca ttagaattat
     4201 agcgggcagc gtcatgaact tctttaaaac catagattcc ggtttccatt tggaacaccg
     4261 tttttccaac agaaaaggca gacattgatt gtccgggtct atttgaatta atcatgtcgg
     4321 tgtgttgtgc aataacaaaa ctaaccgaga aattaaagac aaaaaggaag gcgattttta
     4381 agtttttcat agtgaagctt tctagtagaa tagatataaa aaccaaaaat aagacttttt
     4441 attatttatt taagattgtt agtttatttt tgaagaaggt atttcttatt tttaccaaaa
     4501 ttattgactt atgcagacag catcttttac ggggcttgtg agaacactcc ttattctgat
     4561 agggatatac tatgccttta aaattatagc cagaatctta atgccaatta tattgcgtaa
     4621 aatggtacaa aaggcagaac aaaatttcaa acaacaaact caaaatcaac cccaacaaac
     4681 cgcttctaat tttgataaac aaaaggttaa aaaagaagta ggagaatata ttgattatga
     4741 agaagttgaa taaataattt ctttattaaa aatttaaaag gcatatgaaa tattttcaaa
     4801 aatcgcttcc tcatttatta tccttatttg gatttgtctt tatttcgtta atttattttt
     4861 acccagtttt acaagggaaa aagattttgc aatctgatat tgttcaatat accggaatgg
     4921 caaaagaaca aaatgacttt agagcaacaa ccggtgaaga acctttttgg acaaatgcag
     4981 cttttggcgg gatgccaaca tatcaactcg gagcacaata tccgcataat tatgtcaaaa
     5041 aattggatgg tgccttgcgt tttttgcctc gtccggccga ttatttgttt ttatattttc
     5101 ttggttttta tgttttgtta tccgttttaa aagttgatcc acttaaagcc ttttttggtg
     5161 cgttggcctt cggattatcg acttatctta ttattattct tggcgtggga cataatgcaa
     5221 aagcccatgc aattgcttat atgccgatgg taattgcagg agttatttta gtatttcaaa
     5281 aaaaattcat tgttggaggt ttattaacga tgattgccgc tgcattagaa attcaggcca
     5341 atcattttca gatgacctat tatctgttgt tgctactttt aatactttcg ggatattata
     5401 tttttcaatt tataaaagaa aaagatttta aatcaattgg aattgctttt ggtgttttcc
     5461 tctttgcggg aattttagcc ttgggagtta atgccactaa tttaatggca acagctgaat
     5521 atactgagtt tagtacgcga ggaaaaaatg aattgaccat cggacccgaa ggtgaaaaaa
     5581 tagagaataa atcaggaatg acttatgagt acatcaccga atacagttat ggaattgccg
     5641 aaagtttaaa tttgatagcc cctcgattat ttggtggagc cagtaatgaa aaattggatg
     5701 aaaccagtaa tttatacgaa tttttcgttg gtatgggggc tcctgaaaat gaagctttag
     5761 attatgcaaa tggatcacga gtttattggg gagatcaaac tagcgttgct gctccggcat
     5821 acattggagc tattgtattc tttctttttg tgttgggcat gttcaccgaa aaaagcaaat
     5881 tgaaatatgc ttttttagca ggagcaattt tttcattaat gctttcttgg ggaaaaaatt
     5941 tcccaataat gactgacttt tttattgatt attttccatt gtataataaa ttcagagcgg
     6001 tttcatccat tcaggttatt ttggaattat gtattcccgt tttagctata atgggattgt
     6061 attcgttttt taaattagag aaagatcaac aatggcaagc actttggaaa tcagctgcaa
     6121 tttcattggg tattttagct ttacttattt tagtgaaagg aatgtttagc ttttctactg
     6181 aaagcgatgc ttatttcaaa caggcttatg gagaaattgg tcaacctttt gttgatgcgt
     6241 taaaagaaga tagaagggca atgtatacat ccgatttact tcgttcaatg ggattaatat
     6301 tgactgtttc tgctgttttt tggttgttta taaaaaataa attatcccaa actattgcgg
     6361 tagttttagc aggattactg atggttttag atttatatgt aattgacaaa aattatgtga
     6421 atgccgatga ttttgtaaat gcccgtcagg tagatgaacc ttttcaaccc actccggcag
     6481 atttagaaat tttaaaagat actgacccaa attttaaggt ttttgaaccg ggacaaggaa
     6541 tggctggagg aaggacttct tattttcata aagctattgg aggttacagt gccgtaaaac
     6601 cgcaacgaat tcaacaattg tatgattacc aaattgctaa caataatatt caagttttga
     6661 atatgttaaa cgtgaaatat gtgattcaaa caacagaaga aggccaacct attcctttaa
     6721 gaaatccgga tgctaatgga aatgcttggt ttgttgaaaa cattatgata gttgattctg
     6781 ctgataaaga aataaaagcc ttagatagtt tggatactaa gaaaatagcg gtagtacata
     6841 aagaatttta ttccaattca aaaaataatg cctttataaa agatagctta gcaaccataa
     6901 agttagaaga atataaacct aattatttaa aatatacagc tcaaaacgca aatgatggtg
     6961 ttgccatatt ttctgaaatc tactatccaa acggctggaa agtgactatt gatggaaaag
     7021 aggtgaagga attcagagca aattttgttt tgagaggttt gttaattccg gcaggaaaac
     7081 acacaattga atttaaattt gaaccggaag ttgttaaaaa aggaagttca attgctttgt
     7141 ttagttcgat aggaatgttg atgcttttat taagtggttt atatattgaa tcaaagaaaa
     7201 aaaaggttga ttaatacaaa tgaaaaagaa attttgaacg ggagaccagc agaaattaaa
     7261 aaaatcctaa tcattaccta ctactggcct ccagctggtg gacccggcgt gcaacgttgg
     7321 ctgaagtttg taaaatattt accggatttt gggtatgaac cccatgttta tattccagaa
     7381 aatccaactt atccgattat tgatgaaaag ttaattgctg aagtatcttc aaaagcaatt
     7441 atcatcaaaa agaaaatcag agaaccttat cgttgggctt cttttttttc aaaaaaagac
     7501 acacagaaaa ttagttcagg aataattcca agtaagaaga aacaatcatt agttgctaaa
     7561 tttttactat ggattcgagg aaatttgttt attccggacg cacggatttt ttgggtaaaa
     7621 ccctcggttg agtttctaga gaattatatt caggagcatc aaatagaaac cattattacc
     7681 agtggtcctc cgcatagttt gcatttaatc ggtttggaac ttaaagagca attaaatgta
     7741 aaatggatag ctgattttcg tgatccttgg acgactattg gttaccataa agagttgatg
     7801 ttaacagaaa aatcagaaaa gaaacataaa atattagaaa agaaagtact ctgttctgca
     7861 acaaaaatta gtgtgactag tccatccaca aagattgatt ttgaaaaaat aaccaaccgc
     7921 cccattgctg taattaccaa tggttatgac gttgaagatg tttctaaaac gttgttagac
     7981 gaaaaattta ccttagctca tattggttcg tttttatccg aaagaaatcc aagaattctt
     8041 tggaaagctt tgaaagaatt agtcaaagaa gagcctggtt ttgcatcaga tttcaggtta
     8101 aaactaattg gaaaaattag tcaagatgtt ttagattcca tagctgaatt taaattatca
     8161 aaatatgtgg acaatttagg ttatgtttca caccaggaag caattatgca ccaacgtaaa
     8221 tcgcaggttt tgttactaat tgaaattgat tctgaggaaa cacgatgcat tattcccgga
     8281 aaattgtttg aatatatggt ttctgaacgg ccaattatag ctattggccc aaaagcttct
     8341 gattttgaaa tgattttaaa agaaacaaat actggaactt ttttcggata tgatgaaaaa
     8401 aagcgattaa aaacaacgat tttagctcat tattcgctct ataaagaaag taatttaaaa
     8461 gtttatccgg taggtttaca acattacagc cgtaaaaatc taacccaaca gttagttaat
     8521 ttcataaacg aagcatagtt ttatgggaat cgtactcaat caatccgtta aaaacaccgt
     8581 tataaccttc attggttttg ctattggtgc ggctaatgct ttgtttatgt atactcattt
     8641 tttaggtgaa gactattatg gtttaactgc ttttttattg tcttctgcca atattatgat
     8701 gcctttgatg gcctttggta ttcaaaatac gttggttcgc ttttacgcag aacaccaaac
     8761 ggaagaagat aaatctcgtt ttttaaattt agttttagtg cttccgttgc tcattatcat
     8821 acccgttttt ttaattttat tttttttcta tcctgaaaca gcttctaagc tttcagttga
     8881 aaatccaatt atttatgact atgtatggat aatcccggtg attggcttat ttatgggtta
     8941 ttttgaaatt ttttatgctt gggttaaagt tcatttgaaa tcggttttcg gtaatttttt
     9001 aaaggaagtt ttgttacgta tttttattag tatttttctt tttgccgttt attttggttg
     9061 gataacttcc attcaatttg tttacagttt agtttttatt tacttaacta tgatgttgat
     9121 tatggcattt gtggctttca aaataagaaa accacaattc catttgaagt ttccaaaaga
     9181 gaaaaaagaa gtaattatat attctgcttt tatcattttt tcctccagta tcgctgtgat
     9241 gttgttagat attgacaagt ttatgattgg gcaatatatt ccgttaaaag aagctgctta
     9301 ttattcggtt gctatattta ttgctttaac aatttcagtt ccaatgcggg ctatgcacca
     9361 aattacgcat ccggttacga cagatttaat ggctcgaaaa aagcatactg agctgaatga
     9421 tttgtacaaa aaagcatcta tcacgcttca ggttattgga ggtttaatta tgcttggaat
     9481 tttgacaaat attcatcagg tttatgcttt attgcctgaa aaatatagtg gaggagtagc
     9541 ggttgttttt attatttcaa tttcaaaatt ttttgatttg atgttaggca ataacaattc
     9601 aattattttt aattctaaat attaccgaac cgttttgttg ttaggagttt gtttggttgt
     9661 tttaaccgtt tcgctgaata tgtattttat tcctaatttt ggaatagaag gagctgcatt
     9721 ggccacgcta ttatcgatag gattatatag tttagctaaa ttattatttg tagtaatcaa
     9781 gatggatttg tttccattta caaggaacac attgctttca ctgggaataa ttgggttgac
     9841 ttttatggtt ttttattttt ggaattttcc ttttcatccc attattaata tcattttaaa
     9901 gtcagtttta ctatcatttt tttatttggt tttaaattac aaattaaaaa tttcagtaga
     9961 tattaatgat gtaatagaaa agattttaaa aagaatatat attatgagga ttgaataatt
    10021 gactattaat aattgaaatc ttaattaaat aaaaagtcct gctacttggg gtttcacagg
    10081 actttaatca accaaactta accaaactaa cttttaacta actctactac attcttattt
    10141 tttatctttt ttgtcttcaa ttaatgcttt agtaccatct gctttgtaat agtaataagg
    10201 atcattgtta taggcataat ctgtatttgt ataataattt gaacttccat aataggttgt
    10261 tgtggcatat ccataagcac gggctctcat tttgacctca ccaaacatat ctactaaatg
    10321 acctttacgg ttataaacaa gttccatacc acctatttgt gcaagagcaa atctattgta
    10381 acgcatataa accgttccaa ttctgtttac acggttttgg ctgtcatagt tgataaaaac
    10441 gttaccaatt cttcgaactc taccaaaact atcgtgttct atccgtacac caaagttacc
    10501 aacggctgaa tttcctctgg cagtggctcc acgtttgtaa tagtaaccac cttgactatc
    10561 ttgtggacgg gtattgaaat cgaaatcacc atttaagaaa acgtaaaatt caatttcgcg
    10621 ttccataaaa acgattggtt ctgcgtccat ataatgtaca gctacattat gtcttctgtc
    10681 ggtatcagta tccacaattg tttctgatgc aaaagccaaa cttcctacga gcaagttaac
    10741 ggctactaaa agggtaattt ttttcatgac acattcgatt taactttttt gcctactctt
    10801 tagtttttca gctttcacta agaagtgatt tattaaactt cttgtttagc tattttcaat
    10861 tgccgtgcca aaaatgtttt ttgtagtgtg aatgagttgt taatgatttt taactaattg
    10921 aaagacaaaa gtttatttat acaaaagcta aaactaaaag cggattgatt acaaaaacat
    10981 tttttaatta aactaaaaat ttgctagtat ttgttaaact tttgaattca tacagacaaa
    11041 cgagataatt caacttgatg agttgtttaa tcgactgcag tcaatttatc tttttgtctt
    11101 gatgaactga agggtttgtt ttgattcgta taaggaatgt tattgatgac atgatagttg
    11161 atttaaacga ataagtaaat ttgcctggaa gaagggtcga tagttaaagt ttgcaagtta
    11221 tgccgttatg gtctaagtct ttaataacga ttatttttta gtgggtacaa ctaattcaaa
    11281 ttgaatcttt tcagaataaa tcagttttgc ctgttccttt ttcttttttt tagtcaaatc
    11341 aaacgcttca accacaagtc cattttcagt ataattaagt tcttttcgtt ctgatgctcc
    11401 gtgtagcgtt gcgattctat aattgggaaa atagtttgca tctgcctctt taattttaag
    11461 ttccagacgt agatttactt tctttttctt ttcattggta tagaattgca gcaccaccgt
    11521 tttatataat gaatctcttt ttgtagtctt aaaatcgtag taagttggat actgcctaaa
    11581 tagtttgttt tcttcttttt tttcagaatt aatatactta aaatgaagta cttcaacatt
    11641 cgttgttact tgcctactta ccgaaaaaaa atgattttgc atttgttcag tatccacgag
    11701 ctttgcagtg cgttttccat cagtgttata aaaatacaaa aatatatttg gattgtccgt
    11761 attgctgtat gataccgttt catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnatttgcc gagctagaat agacggtcct ttttctgaca tagtcttttg taggcatctc
    11941 atacgtggtc acaaaatcaa atgtaaagct ctgcgaaaaa gctagagttg ataataagac
    12001 aaagaaaaca caaaaaatgt ttttcattta atttcaatta gaaagataaa tctaagaaat
    12061 ttatctcaac ttttccttta aatatttccc ggtaatcgat tttgggtttt tagtcacatc
    12121 ttccggtgaa ccaaaagcca tgagtttacc accattttcg ccaccttcag ggccaatatc
    12181 gatgatgtaa tcggcacatt tgattaaatc aagattgtgt tcaataacaa taatcgaatg
    12241 acctttttca atcaaggctt caaacgaggc caatagtttt ttaatatcat gaaaatgtaa
    12301 acccgttgta ggttcgtcaa atacaaaaag ggctttgtct tttgtagttc ctttgactaa
    12361 aaaggaagca agttttatcc gttgagcttc accaccggaa agagtagacg atgactgtcc
    12421 taattgtaca tagcctaatc caacatcttg aagcggttgc aatttttgaa caattttagc
    12481 ctgattttgt tctttaaaaa aagctaaggc atcgtcaatg gtcatggtta atatgttgtc
    12541 gatgtttttg ccttcaaatt gaacttccag aatttctttt ttgaaacgtt ttccaccaca
    12601 ggtttcacac tctaaatgaa catcggccat gaattgcatt tcaatagtta cttcgccatc
    12661 acctttgcag gtttcacaac gtcctccatc aacattaaat gaaaaatgtt tgggtaaata
    12721 gcctcttatt ttagagagct tttgtttagt aaataaatcc cttatatcgt cataggcttt
    12781 gatataagta accggattag aacgggaact tcgtcctatt gggttttgat ctacatattc
    12841 aatgtgcttg atatgtgaaa aatttccacg tagttcagta aattgtcctg ctttgtcact
    12901 cacgccttcc aattttttct gtaaagcagg aaacaatatt tttttcacca atgtactttt
    12961 tccactacct gaaaccccgg taatcacggt taaacaatct aatggaaaag tgacatcgat
    13021 attttttaaa ttattttcac gggcaccgat gacttcaata tgatttttaa attttcgccg
    13081 ttttttagga atttcaattt ctaattcgcc gtttaaatac ttagcagtaa gggaatcagc
    13141 ttttagaatt tcattaaaat taccttcagc aactaaatat ccaccaagcg ttccggcttc
    13201 cggcccaata tcaataattc ggtcggcagc tttcataatg tcttcgtcat gctcaaccac
    13261 tattaccgtg ttccccagat ttcgaagatt tttcaaaacg tcaattaatc gctctgtgtc
    13321 tttcggatgt aagccaatac tgggttcgtc taaaatatac atcgagccaa ccaaactact
    13381 tcctagcgaa gtggctaaat tgatacgttg tgattcacct cctgaaagtg aggcagagtt
    13441 tctgtttaag gtgaggtaat ctaatcctac tttttgtaaa aaggataatc ggctgtttat
    13501 ttctactagt aatcttttag caacttttaa atcatattct gaaagttgta attctgtaaa
    13561 gtaagaaatt aggtttttaa ttggcaaatc tactaaatca gagattgttt tatttcctac
    13621 tttgatatag tttgcttctt tccgtaaccg tttgcctttg caggtagaac attttgtttt
    13681 accacggtat cgagacagca tcacgcgatt ttggatttta taattttttt cttccagctc
    13741 atggaaaaaa tcatttagac ctgtaaaata actgtttccg ttccaaatca actgtttttg
    13801 atcttcactc aattgataaa atggtttgtg aataggaaaa tcaaatttat aagcgttgtt
    13861 tactaattgg tcgcgatacc agcccatgct ttcacctcgc cagggaaaaa tagcattttc
    13921 ataaacagac aaagcagtat ttggaattac caattcttca tcaataccaa ttacatttcc
    13981 gtaaccttcg caacttggac aagctccgta gggattattg aaactgaata aatgaatatt
    14041 aggttctaaa aaagttaagc catctaattg aaaattagtg ctgaatccaa ttctatttcc
    14101 ggttaaaact tcttgcaaaa aacagtctcc ttttccctcg taaaatgcag tttgaacggc
    14161 atcggctaat cggtttaaaa attcttcatc acttttaact actattcggt caataatcag
    14221 aagaatatct ttattgtcca atttatgctg ttccaattca tctaaacgaa cagtttcatt
    14281 atccactaaa actcgaataa aaccctgttg taataaaact ttcagtttgt cttctaattg
    14341 tcttccggtt tctaaatgaa taggagcgag gagaagccat ttactatccg gttcgaaggt
    14401 agaaattgtt ttcacaacat cagttacggt atgttttttt acttcgtttc cagaaatggg
    14461 agagaatgtt ttaccaattc gggcaaagag caattttaaa taatcataaa tttccgttga
    14521 agttccaacg gtagaacgag catttgtagt gtttactttt tgctcaatgg caatagcagg
    14581 agcaattcct ttgatgtatt ctacttttgg tttatccaat ctacctaaaa actgtcgggc
    14641 ataggaggaa agactttcta cataacgacg ttgaccttca gcatataatg tatcaaaagc
    14701 taaacttgat ttacccgaac ctgaaagccc agtaataaca accaattgat ttcggggaat
    14761 agctacatca atgtttttta gattgtgcag ttgtgctcct tttatcagga tatttttttt
    14821 aggctcaagt aacgcaaatt cttccggtgt catagtaaat ttcaaagaat cgcaaagata
    14881 gtgatttgaa agatgaatta aaacaacggc agaaagctat ttttctataa aaataggatg
    14941 attttttgcg gttttccgca tttattatat tttgagtgtt aaaatttcag ttaatatttg
    15001 tttgttaaat taaaattgta ttacatttgg gatataatta atcttaaatt aacaattgcc
    15061 tatcgtataa aaatactttt tttagaaaaa atttactcaa aacacgaact ctaaaaggta
    15121 ttattatggc tactgttaac ctccccgacg cattattggt taagaattac attgctggtg
    15181 acgaaaaagc attggctatt cttattgaaa gacatcaatc taaaatttat ggatttattt
    15241 attctaaaat tgcagaccgt gatataactg acgatgtttt tcaagatact ttcattaagg
    15301 taattaaaac cttgaaatca aattcctata acgaagaagg aaaatttttg ccttgggtga
    15361 tgcgaatcgc acataatctt gtagtagatc atttcagacg caataaaaaa atgccaatgc
    15421 atcgtgaaac tgaagaatat tctatttttt catacatgac agataattct atgaatgtgg
    15481 aaggaagaat gattactgac caagtggaag atgacttaag acgtttatta gatgaattac
    15541 cagcagacca aaaagaagtg ttagttatgc gtatgtatca agacatgagt tttaaggaaa
    15601 ttgctgaatc aaccggagtt agcattaata ctgctttagg aagaatgcgt tatgctttaa
    15661 tgaatttgag aaaagtaatt gaaaaaaatc aaattatttt aaccaattaa acaataattt
    15721 tattggagtt gtcgttgtat aagaaaacaa acatttttta tatggcaaaa ctttacacta
    15781 gtaaaaaatt agcgactcca aagatgttac ctaaaaaaga aaccataagt tttttgctca
    15841 actactccaa ggcactgagc attgttaagg taggaaacat gactttcgaa accatcgcta
    15901 attaaaggaa catcccgatt aaagtcggga tgtttttttt tctatttctt ttaaaannnn
    15961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttgg tcatttttgt acttttataa
    16081 ctgttaacaa tttgaaacaa agttaaacgt taaacttttt aaataaaaat gtaatgacaa
    16141 cattaaaaaa aggcaatcaa gctccggctt tttcaggaaa agatcaagat gggagtgtgc
    16201 actccttatc agattacaaa gggaaaaagt tagttgtatt cttttatccg aaagcaaata
    16261 cacccggttg tacggctgaa gcatgcgatt tgagagataa ttatgctcga tttgaagcag
    16321 gcaattatac gttattgggt gtaagtgctg ataatgccaa agcacaagga aatttcaaaa
    16381 ataaatatga atttccattc cccttaattg ccgatgaaga taaatcggtt attcaagctt
    16441 ttggagtttg gggtcctaaa aaatttatgg gaaaagaata tgatggaatt catcgaacca
    16501 cttttgtaat tgatgaaaat ggaataattg aggaagttat ttctgaagta aaaactaaag
    16561 cccatgcaaa tcaaattttg ggataaaaaa aaggccgaat gtaaaaatca acattcggcc
    16621 tttttaattt atttgctatt tattttgttt gttcaactat aatttttttg ggatcataac
    16681 cgaataagtt gtctcttttt atgatttctg caactccttt tggaagcatt tcttcccaac
    16741 ccggtgtgcc ttttgcaatc atttttaata catggcgaga ataaatttct aaattattgt
    16801 gattaaaatc ttctatatca acaacttttc cgttgaattt aaagaattta tacaattctt
    16861 tcattcgtgg atgtactttc agattttcag agttcatgat tgtgccatct tcacttttca
    16921 ttggatacaa gaatacctta aggtcacgat agaataattt cccaaaagct tctaaaattc
    16981 ctccgcttaa atggcgataa tatttttcgt caaaaatatc taccaaattg ttaaccccca
    17041 tggctaatcc cattctagct tttgtatagc ttgagaaata ttctacaacg cgataatatt
    17101 cctggaagtt agatatcatt acatattgtc ctaatgaaca taataattct gccctgtcca
    17161 tgaagtcgcg ttcgtctatt tcaccttctg atcttaagtt tgaaagagtg atttcaaata
    17221 ttaccaaggt attatcagca tcaactttac tttcgttcaa aaacatttca agtgattttt
    17281 catacatgtc catattcacg cttgtaactg gacgaaaact tcctcgtaaa gcaagaatat
    17341 tttttttgta taaaatggaa gccggtaaaa tatttgttcc atcagggcca aacattacgg
    17401 catctgtcat tccgttttta accaattgta aactcattaa ccgattatct acattagcaa
    17461 aacgcggtcc ggaaaagtta atggtatcaa tctctaattg gtctttatct aagtggtcat
    17521 ataaataacg aagcaatttt tttgggtcat tgtatttata aaaagcacca taaataagat
    17581 tgactcctaa aattcctaat gtttcttgtt gtaaacgggc atctgtttct ttaaaacgaa
    17641 tgtgtaatat aatttcatta taatcttcat ccggttcaat ttggtattta atgcctaccc
    17701 aaccgtgtcc tttaaattgt ttagcaaaat caatagtagc gacagtatta gcataactga
    17761 aaaacatttt attgggatgc tttacgcgat ccaatcgttt ttcaattaag tttacttcgt
    17821 gatgtaacat tttttttaaa cgactttctg ttacataacg accgtctttt tcaacaccat
    17881 aaatggaatc gctaaagtct ttgtcatagg cagacatcgc ttttgcaatt gttcctgatg
    17941 aaccaccaga tctaaagaaa tgccgaacag tttcttgtcc agcaccaatt tcggcaaagg
    18001 ttccgtatat gttttcattc aaattgattc gcagagcttt atctttaatt gaaggaatct
    18061 gctcaatcgc cttatcaccc tttaatttta tttccatagc atatctttcc atatttcctt
    18121 agtaatacat tgcaaagtta acaaatatca taccgtaata aaagattaat aatgctattt
    18181 ttgtgaaaat ttaacgtctt gaaggtttat tttttaggta ccggcacatc tcagggtatt
    18241 ccagttattg gaagccatca ttctgtttgt aagagtacgg atgttaaaga taaacgcctt
    18301 cgggtttccg tttggattca tgatgaaaaa agctccatag tgattgattg tggaccagat
    18361 ttcagacagc aaatgttaac ctgtcgatgt caaaaaatag atgctatttt gtttactcac
    18421 gaacacgctg atcatactgc cggtttagat gacatcaggc cttttttttt caaacaaggg
    18481 gatattccaa tttatgctca taaacgtgta attgccaatt tgaaaaaacg atttgattac
    18541 atttttgaaa ctgaaaataa atatccggga gctccatctg tagccataca tgaggtagta
    18601 aaaaataata cttttaaaat cggtcaaaaa aatgttattc caattcaagt aatgcatggt
    18661 tcgcttcagg tttttggtta ccgaatagat tcttttgctt atttaacgga tgttaaaact
    18721 atagaatcaa gtgagttgga aaaattaaaa aacctagatg ttttagtagt caatgccttg
    18781 cgtgaggaac ctcatgaaac ccattttaat ttgcaggaag ctttagattt tataactttg
    18841 gttaatccta aaagggcata tttaacccat attagccact tattaggatt tcatgaagaa
    18901 gtgcaacaaa aattaccatc gaacgtttat ttagcttatg ataaccttga aattataatt
    18961 taataatgaa aaagtcttta tttctctatt tatttattgt ctccttttta atgatgattt
    19021 ttacttatat gtattatagt aaaaaagtaa aatttgaagc agagcgattt gaaaaactta
    19081 aagttaaaat gaatgacagc attcagtcgt taaataatca ggtttttgaa gctaattatt
    19141 ttacactttt gaataatgat aatgcacaaa actatttaga agattactcc atcccaaaat
    19201 taatttcaaa aattgaacaa aatttgatgg aattaaatga taatccggaa ggaaatcctt
    19261 tagttgaata tggaaaaata ggagaccaaa aattcataat aaataaaatt aaagttataa
    19321 atcaccgttg gattattgct gatttcagca atggtgatct ttggggagag gttttattgc
    19381 agtacttcat tgaggaagat gagcaattga cttttaaaat tattcaatct actttatatg
    19441 tcaagaaata atgttttgta tctttaactt tctttattaa tttgtagata tgaaaaacag
    19501 tttgctttta ctttttgtgg tattcaattt agtttcctgt aaaaaatttg atcaggaaat
    19561 cgaacctgaa atagccaaga aacaaacttt tatagatact gttgaaaatt cagataaaga
    19621 tgaaaatggc tgtttaactt ctgcaggata tgtttggtcc cagctcaaca aagaatgtgt
    19681 taaaatatat caatctgcca ttattttata tccgcatgct aatcaaacca atgaagatga
    19741 aactttaaat gcctatgttg tttttagtaa agaaggagga aatgaagctg aaatattttt
    19801 tcccaatact gaaaaatcac atctttttat tcgcgaaact gaaggacagc catggaaata
    19861 tcaagattgg caattgattc cctggaaagg atttattctt aaaaaaggag aagaaattaa
    19921 attctccgga gatggtgaat ttggtcccaa agttaccgga agcgataaaa tagaaaatta
    19981 gtttatttat tttctaacaa ccattttctg aaatcaaaga aattttgagg cgaaactccg
    20041 tgaccaattg gatattcttt atacacaaca tcaattccga aattttccag taattgaggt
    20101 gtttttctag cccattctac cggaataact tggtcnnnnn nnnnnnnnnn nnnnnnnnnn
    20161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20221 nnnnnnnnnn nnnnntcacg agattgacga gcttgattta aatctgaaaa cttgttttca
    20281 tctgcatcaa aattaatagc ataccaagca aagcttccat attgcatgtc ataaggagcc
    20341 tgagcagaaa ttacaaaata ttcttccgga agttcagaag caaaggagaa caaatctgct
    20401 tcattgctgc cgtaaccgtg taataataat agaagaggat ttttttcttg aataatttta
    20461 ggttcacgaa ccaaataatg taatgacatg attatagatt tttaaatagt ttttggtaaa
    20521 atccacctaa caaaggaatg cttttctgtt gaccatttaa agctcccata aatccataaa
    20581 tccaaagtat aaaaaagaat aaataaaagg cagaagaaac catccaatca tcgaaatagc
    20641 caataacata gcccaatgca aaaaaagtta taaaaattcc caacgcttga cgaatatgaa
    20701 aactggcaaa ggtacttttt gattcactat tcatgaaaat tgcaattaca gcaccaataa
    20761 tggtaaggta tgcaacgatt gcagcatttt tattttgttc catattatct tattactaat
    20821 tttccgttat tataaattcc atatactttt cctttcattt cctgtcctaa aaaggcagtg
    20881 ttttttgatt tggaaagtac attctcttta gcaaaaatcc aatttccatt tggattaaag
    20941 aaagataaat ttgcaacttc accttccttt attgaattgc tatttaatcc aaaaacagct
    21001 tttccggcag tcaattttgt gacaattact tcggtaggta aaacagtttg taatgctccg
    21061 aaagcatttt ctaacccaat tgtcccattt ttagccatat caaactccat ttttttatgt
    21121 tctatatcaa ttggattatg atcgcaagta atcatgtcaa ttgttccgtc taacacacct
    21181 tgtattaaag ctgtacgatt aaaatcatca cgaagaggcg gactgacttt aaatcgggta
    21241 tcaaaactat ctaatttttc atcggttaaa actaaatgat gtattgctac actagaagtt
    21301 atgttcaaac cttttgcttt tgcttcacga attaagtcaa ccgattttga agttgatatt
    21361 gttggaatgt gcatttttcc accggtatat tccaataaaa acaaatttct ggcaaccatt
    21421 aattcttccg ctaaagttgg gatacctttc agtcccaatc gggtagaaac aattccttcg
    21481 tgaacaactc cacttccttt tattttttca tcttgcgaaa aggcaattat taatccgtca
    21541 aaatcctgaa catattgcaa tcctattttt aacaaattag catttgaaat attctttcca
    21601 taatcgccaa aggcaattgc tccagcattt ttcatgtcaa acaattcggc taaatctaag
    21661 ccttctgcat tctttgtgaa agctccaatg gggattaaat cgacagcatt ttcttttgct
    21721 ttctctttaa caaatctaat ttgagcttga ttatcaatta taggcgaagt atttggttgc
    21781 aaagcaatgg cggtaaatcc actttttgca gctaccgata acccatttgc aattgtttcc
    21841 cgctcttcaa atccaggttc tcctagagaa actgaggtat caaaccaacc cggggaaaca
    21901 tgtaaatttt caaaacttat ttcctcaaaa tcagatgttt tttcaagatt cttcccaatt
    21961 ttgattatac ttgagccaga tatctttaaa tcaatagttt gattatgata tggactattc
    22021 tgatcaatta ttgttgcgtt gcgtatgatt aggttcattt cacaaatttt tgaatgagta
    22081 cttccaagac caaaaataat aaagttaacc acacaaacca tttccaaaca gcattatcta
    22141 ttcgattagt ttgtagggta ttaaaaacgg tttccattga attaccgttt tcatattttt
    22201 caaccaaatt tgaattatct aaacttaaat cactttcagt tcgaggataa ttaaaactaa
    22261 tattttttaa aattgaatta tctttaaaag cattgtaatt tccagcaatt gttggacttt
    22321 caacaaacga gagtttaact ttattgttta ctatttgttg ttgtggtata aatttttccg
    22381 ctttttcatc tgttgccgaa attactgaaa taatctcatc ttttgataaa agttggtcaa
    22441 cagtaaccgg agttgaagta cctattggta aagcatttat gcctgttttt acactttgca
    22501 gtgccatatt ataaaatgtt ggcactatca atggcgagtt tttaaaattt gaattgtctt
    22561 tatggattgg ggctgaaaat acataaactg aactcaaagc ggttgaaaca gcagttaaaa
    22621 acggactttg gtcttcataa cttaaaatag caggataagt gcttattagt tcaaaaccgg
    22681 aaaccgtttt aggatattga aaattgtcca cttttttttc aaaaacacca gaatataatg
    22741 ggtgagcgaa tgaaataccc gtaattaatt tctccttttg tattgaatta ttcatcgaaa
    22801 tagcaccaaa atttcttaaa aaaaagtttg aattagaaat gctagtttct gaagatggaa
    22861 taaaaatcaa gtttccacct ttttcataaa aagattttag tgtagtttgc aatgcttgtg
    22921 gaatttcttt aagctcattt aaaataactg catcttgttt ttcaagtaga ttataatcta
    22981 acgattttaa ttcaaattga gaaaatataa attcattttc agtatatatt tttgttagaa
    23041 attgattttt ttcaacttga ccaatcgcta aaacatttgt tttttcaggt ttagaaatac
    23101 tgaaataata gtaattgtca taaaccaatc ctgaatcatc caaatataca taaccattaa
    23161 aatctgcttt tggaatagtg aagaaaagat ctttctcaag ttgattcact ttaatttgag
    23221 ttttagcaat aaattgtttt tgattataca aagcaaccgg aatttcaatg tccgattttc
    23281 cataagattt tatttttaca cctatttcgt aaaaattatc taatgtttgg tgtaaataca
    23341 cactatcaac agaaacatta tttttttgtt ctgaatttgg aataataaag aaaggattat
    23401 acgttttgtc aagtggtttc agttggttct cctgtaaatt aattgcatcg gttatgataa
    23461 ctaaatcttt attataaggt gtttttctag atttaatttt tgtcaggctt tgatctaatt
    23521 gaaacgaatt gacactataa ttcaaattca tcaattcttt ctgtatcgat ttgatatcag
    23581 tattccaata aatagcatcg ttggttaaca atgaaaacgt tttgttctcc ggtatatttt
    23641 ctaataattc ctgtacggca cgtttgagca gttctccttg ttttcctttg gcttgcatac
    23701 taaaggaatt atcaagaatg atataaagtt cattatttac tcctttacta tctttcgctt
    23761 taaaaaaagg ttgtgcaaag gcaaaaatca aagctgttaa taaaaataaa cgggtaagta
    23821 acagaagcca tttcttaagt tgtgaacttt tacgcgtttg tatagaaagc tctttaagaa
    23881 attttacatt ggtgaagtat tcctttttaa aacgacgaag ttggaataaa tgaaccaaaa
    23941 ttggtaaaac caataaaaat aggaaataca gaatttcagg atgtttaaac tgcatttaat
    24001 gaaagttttt caaagatagt ttttatattt taatagtaca aaagaattac aatgaggtct
    24061 gtagaattaa gtatatagaa tacttcgcct acttatttat tctatttttt ctttctttta
    24121 gcatctcttt tcttctctac accgcgattt ctggattctg tttttctggg agtacgtttc
    24181 ccgggaccac ccaaattaac ctttttgttt ttgtctttct tgtcatgaaa tgcttcacca
    24241 ccctcttttt ttggtccttt cagaagcact ttcatttttc ttttctcttt ttcgaattcg
    24301 agtatttttt cctcgatttc aatattgggt aatgctaaaa accgaatttc tttttccatt
    24361 aaaatttcag cttcaatttg aatttcttct tctctcggac tcacaaaact taaggcagta
    24421 ccggttttgt cggcacgacc tgttcgtccg atgcgatgca cgtattgctc aggaacttcc
    24481 ggaaattcca tattaacaac atgtgtaata tctgaaatgt ctaaaccacg agccattaca
    24541 tctgtggtta caattcctct gatttctcct gcttgaaagg aagccatagt attcaaacga
    24601 taattttgtg atttattgga atgaattaat ccgaattgat caggaaaatc ttcttctaaa
    24661 gaagatgcaa ctaaatcggc agttttttta ttattaataa aaattaaaag ccttgacata
    24721 ctttcgtctg tgtgcaaaag ttctttcagc aaattaattt tagttaaaaa attaggtact
    24781 ttatatccga gttgcgaaat ctgttccaaa ggagttccgg atggagctaa agaaacttca
    24841 ataggaaaat caaaatattc gtctaacatg tcatctactt cgtctgtcat tgttgccgaa
    24901 aatagaatat tttgtctttt actttttaac atcgttaaaa cggatgtcac ttgaaatcga
    24961 aaacctaaat tcaacatttc atcaaattca tctatgatta atttttgaat ttcatcaaat
    25021 cgaatcacat tgtccaaagc taaatccatc actcgaccgg gtgttcccac taagatgtcg
    25081 acaccttgat aaacagcagt tttttgagta ttaatattca ctcccccata aacacccaat
    25141 gttctaacag acatgtgttt tgtcaatttg acaacttctt cagcaacttg cactaccaat
    25201 tctcgggttg gaactaaaat taaaacccgt ggtgtgtgaa tattttgaaa cttccattgt
    25261 tttaaaatag gaagtaaata ggcaaatgtt ttaccggttc cggtttgagc aattcccatc
    25321 atatcacgac cggataaaat aaccggcatc gattttattt gaataggagt aggggttgaa
    25381 aagcccattt catccaatgc tttttgtaat gatttgggta agtcaaaatc caaaaaagtg
    25441 ctcataaata catagttttt gcaaaggtac gcatttttga aggaagaata gaattgatgg
    25501 atttagannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntag tttttgcaaa
    25621 ggtacgcatt tttgaaggaa gaatagaatt gatggattta gaagaaaatg attgtctgac
    25681 ttttactttt acttttaatt taatttttta aaaagtctaa taattatagt tgccaaaatt
    25741 gctccaacaa atgctaaaaa catgtctttt tgagcatccc aaacatcccc ttgtgtacct
    25801 aaataagcat gtccttgagc tttaaaaaag atgtctgcca cagcccattc taatatttca
    25861 tataatgctc caatagaaat agataattct attggcagca tccaagaaat tgttttaggc
    25921 aattttaacc aatgtctaaa taattcctgc aaaggataag ttagtaagaa accaaatcca
    25981 aaatgtacta ttctatcata atgattgcgt tcagtattaa aaatatcttg taaccaatag
    26041 cctaatggat tttctgcata agtgtatttt gaaccatata catgtaggca taagaaaata
    26101 aaaatgaaaa aataggacaa atcactaaag ggatactttt tatagctaaa agataaaaat
    26161 aagagcgata aaatggtcaa tgtgttttca atccaccaat tattataatc tgaagttcct
    26221 ataaaagtcg atacccaaag cataaaaaaa agcataatat accctatcaa ccatttattt
    26281 ttaggaaaca attctcttgt aacggattct gccaaggtga atttcatgaa agtggaatag
    26341 gattgaatta aataaattga ataagaccaa attacagttt tttgttgaaa taaagatgtt
    26401 ttttagaaac tttgaatagc aagaattagg ttagctaatc aaatttacct cgttgatttt
    26461 aaaaaccttc aaaaactcct agaccaaaca aggcaaaatc atattttaca ggatcttttg
    26521 aatctaaaat tcgcaaccga gtatccaatt caaaaagtgc ttttccatca ttttgttttc
    26581 tatgaagcaa tcctaatttc ctggctacat ttccagaatg aacatcgagt ggacatgata
    26641 atttactggg tggaattgat ttccaaatac ctaaatctac accggtttta tcgttacgag
    26701 ccatccaacg taaatacata ttgatacgtt ttgctgctga attattcatg ggatcagaaa
    26761 tatgtttttg tgttctattt aaatgttcaa tttcaaaaaa ggttttttta aattccgaaa
    26821 tacttaattg taaagaatca ttttgttgat tttttgcaaa aacggcttct aatccggaat
    26881 gattttcata aatatttttt aaccctttaa taaatgttat acaatcaatt ccgttaaatg
    26941 ttcgatgaac gaaattgttt actgtttcca attgattatc agtatgattc atgataaaat
    27001 catagggcga atttcccaac aaatacatta atttcaaccc atttttgata atcattttac
    27061 ggtttcccca cgaaattgta gcagcaagaa atcctgaaat ttcaacatct tcttttaatt
    27121 ggtatcggtg aggaatttga attgggtcac tttcaataaa gttgggatgg ttgtataaaa
    27181 caactttttc atctagaaat tctttcaatt cttttttggt catagattaa cctttgattg
    27241 ggttcaaatt ggttaaatca atccaacctg cgtttttttc aaataagcca tctaccataa
    27301 ctaattttcg atcagccata tttgctaggg tttcattatg agttacaatt acaaaagttt
    27361 gaccgaactc atttcttaat ttaaaaaata attcatgtaa gttttctgcc gaagcggtat
    27421 ctaaatttcc ggaaggctca tcggcaaaaa taactgcagg tttatttatt agagctcttg
    27481 ctactgctac acgttgttgt tctccaccgg aaagttctcc gggtttgtga tccatgcgat
    27541 gcgataatcc taggtattct aataattttt ttgcctctgt ttcagcttcg gttttgcttt
    27601 ttcctgctat aaaagctgga atacaaacat tttctaaagc tgtaaattca ggtaggagtt
    27661 gatgaaattg aaaaataaac ccaagtttta aatttctaaa cttagataat tctttgtcat
    27721 tcatttctaa aacagattgt tcatcaatga ttagctctgt tccagcttgt ggtgttggtt
    27781 tttctaacgt tcctattatt tgtaataagg tagttttacc ggcaccggaa gctccaacaa
    27841 ttgaaacaat ttcaccttta ttcacaacta aatctactcc tttcaaaaca tgaagttggt
    27901 cgaatttttt atgaatgttt tttgcttgaa tcatatcaga tagttttcac aaagtaacaa
    27961 agattttaag gattattaaa cctatcattt ctatttatcg ttgtataaat aataaattta
    28021 agttaacacc actaaaactg tttaatttta tcttacattt gttaaacaaa aaattagtta
    28081 tgaaaaagtt attctttatg atgattgcat ttgcaacaat ttcatgtcaa tcacaagata
    28141 agaaatcaaa ttatagccaa gttaaagaac caattaaaat taaaatgaca aacgaaatgg
    28201 aaattgccac ttttgccggt ggatgttttt ggtgcacaga agccatattt ttagaattaa
    28261 agggtgttga aaaagtattg ccgggttaca ttggcggtac tgttcaaaat ccaacgtata
    28321 aagaagtttg taatggtaca actggtcatg ccgaagcgat tcaaattact tttaatccga
    28381 aaataatttc ttacggggag ttgttggaaa tattttttgc cacacacgat ccaacttcgt
    28441 taaatcgaca agggaatgat gagggcactc aataccgaag tgaagtgttt tattatacag
    28501 ataatcagaa gcagcttacc caagattata ttgcattaat gactactgaa aaaacgttta
    28561 aaaatccaat tgtaacaaga gtgagtcctg cttcagaatt ttttattgct gaagattatc
    28621 atcaaaatta ttataattta aataaaacgc aatcgtattg tagttatgtt attacaccaa
    28681 aaattgaaaa attgaaaaaa aattataaac aaaaactaaa aagtaatttt taaaatattg
    28741 aatctaaaat atatttccaa caataaaaaa atataaaact atgaatacct taatagaaaa
    28801 agaattattt gaaattttag gagatgaaca tcatatgaca tcagccgaaa cgcctttgag
    28861 accggatgca tttgataaat cggatgttga aaaaatggca actattgaaa agcattttca
    28921 tattattatg gaagaaatgg gactcgacat gacagatgac agcctgagag gtaccccgca
    28981 tcgagttgca aaaatgttta ttcaggaaat tttcagtgga ttaaatccgg cgaataagcc
    29041 taaaatatcc gtttttgaaa atagttataa ctatgataaa atgttagtgg aagctaatat
    29101 tagttttaat tcaacttgtg agcatcattt tctacccata attggtaaag cacatattgg
    29161 ttatgtttct aacggaaatg taataggttt atcgaagtta aatcgaattg tggattatta
    29221 tgctcgtcga ccacaagttc aggaacggtt gattatgcaa atttttaatg aattaaaatc
    29281 agttttaaat accgaagatg ttattgtggt aatggaagca gagcatttat gtgtttcgag
    29341 tcgaggaatc aaagacagct ctagctttac atctactatt caatatggcg gaatttttaa
    29401 tgataaagaa aaccgtaacg acttttttaa tttattgaaa caggataaat aatttggcat
    29461 tacttttgtc tttgcttgtg cattcaaaaa caaaaataaa tgacaaaaga gatcctggtt
    29521 aaacctgtaa aagacactaa aaaaagagat ttagtacttg gtttactatt tgatgcgatt
    29581 ggttttatat cctacgctgt tcctttttta ggcgaattaa ccgatttagt ttgggcaccc
    29641 atagcaggtt tgattttact gaaaatgtat aaaggaactg ttggaacagt tggtggaata
    29701 accgttttta ttgaagaatt gattcccggt ttagatttta ttccgacgtt tactttaact
    29761 tggatttata cttattggat aaaaaagaaa taattactca aaaagcttca tattatatga
    29821 agctttttta attttatact tagctactca atttttctct tctcgaataa atctgcaatg
    29881 aaacccaatt tcattccacg aagcattttt ttttcaaacc accagaagaa tttaaattgt
    29941 ccaaaaataa atcctacgaa aactaaaagt acttgataaa ttggaaaaat agctaataat
    30001 ctgattggta atataaagcc tcctaaattt tctcttgtta aaccaatgta atttgtaaaa
    30061 ggctttgata aatatgctga taatgatccg gttatggcaa aaacaataaa gatgattata
    30121 agttgaaaat tagaggttat tccccaacgt ttttttaatt tttccatcga ttaaagtcta
    30181 ggttcaaatc cccctaattt ttgcttatat ttttgttgaa aataaacgaa atagttataa
    30241 agaatataat ttacttcata gccatagtta atattgtttt ggtaatcaat ttgcatttca
    30301 tagagcattg ggtcatatcg attacgatca aaaactcgtt gattataatt tacaacccaa
    30361 atccgatttc tattttctaa gtaggattga tcataataat ttcggggttt tgctgtagcc
    30421 aataaccaag aattaaaacc gggttcaatg attataattt catattctaa ttcatcatta
    30481 gcaattcgaa tagtatcatt aacgttagga ttattttcag ttgaagccat accatttttt
    30541 gagccactgc aagagcaaat aatatagttt agagcaatta aaataaacca ttttttcatt
    30601 ctataaagat aagaaaaaaa caaaaaaata taaaacatta tagtcttgat ttaacaaaaa
    30661 aaccatctca atggagatgg tttaaatgct atttccttta cttatttttc agtttttaat
    30721 aagctgataa tctgttctgc taattcggtt ccaattctat cttgagcttc ttctgttgct
    30781 gctccgatat gaggagttaa tgaaattttt ggatgcatta atattttaat ttccggagtt
    30841 ggttcatttt caaatacgtc taatccggca aataatactt ttccttcatc taaagcttca
    30901 agtaatgcaa cttcatcaat tacaccacca cgagcagcat taataattcc aacattatct
    30961 ttcatacctt ttagttcatc acgaccaatc acataaccct cttgagcagg aacgtgaagc
    31021 gtaataaaat cagcatgttt aaataaatcc tccattggtt ctgtttgaat atcaacattg
    31081 ataaattgac cattatagaa atcaacgcga atgacagctt cacctacata tttatcagaa
    31141 gcaataactt tcatccctaa tcctaaggcc attttagcaa cagcttgacc aattctacca
    31201 aaaccaatga ttcctaatgt tttccctcgt aattcgattc cgttagcata ggcttttttc
    31261 aaaccatcaa aattcgtatc gccttctaaa ggcatatttc tgttggcatc atgtaaaaag
    31321 cgaacacctg aaaataaatg agcaaaaact aattcggcca ctgattctga agaagatgca
    31381 ggagtgttaa taacatgaat tcctttttca cgggcataag caacatcaat attgtccatt
    31441 cctacaccac cgcgaccgat gatttttaaa cttggacaag catcaataat atctttgcgg
    31501 actttcgttg cactacgcac taaaattact ctaatttggt gtgtattaat aaaattggcc
    31561 acttgttctt gtgcaacttt ggtagttata acttcaaaac cacctttttc gagagcttta
    31621 attccacttt tagaaatacc gtcattggct aatactttca ttttttaatg attttatgag
    31681 atgctgagct tctgagtttc tatgtaacta agtcgctgag atctatgttt atatttttat
    31741 ctattttctt tcttctaact tcttttttcc aattctttca taacatcaac taatacttga
    31801 acactttcta aaggaagagc attgtacatc gaggcacgat atcctccaac agaacgatgt
    31861 cccggtaatc cagatattcc agctgctttc cacattgaat caaataaagg agcatgtgct
    31921 tcatcgttta ataaaaaggt cgcattcata ttacttctga attcaggttc agcagttcct
    31981 ttgaataatg ggtttctgtc tatttcagtg taaagtaagt ttgctttcgc ttcattcacc
    32041 ttttcaatag ctgaaatacc tcctaaattt tttaaccatt gtaaagttaa aagcgaggca
    32101 tataccggaa aaacagcggg tgtattgtac atgctttctt tatcaatatg ttgtttgtaa
    32161 tctaacatat ttggaatgtt ccgacctgat ttgcctaaga tttcttcttt tacaaccacc
    32221 aatgttgttc cggcgggacc catatttttt tgagctccgg catagatcaa atcaaatttg
    32281 gaaaaatcta aggtacggga aaaaatatca gaactcatgt cgcataccat cggaatatcc
    32341 agggttggaa attctttaat ttgagttccg aaaatagtat tattggatgt acagtgaaaa
    32401 taatctgcat cggaaggaac atgatatccc tttggaatat gattataatt ttccgctttt
    32461 gaagaagcaa ctacaacggt ttctccaaag gatttggctt ctttaatagc tgaatttgcc
    32521 catgtaccgg tatctaagta ggcagctttt ccattaactt tcattaggtt ataaggaacc
    32581 atcaaaaatt ctaaactggc tccaccatgt aaaaacaaag cttgataacc ttttccttgc
    32641 aatcctaata attctaaggc taaggctctg gcttcatcca taacggcaac aaaatctttg
    32701 ctacggtgtg aaatttctaa tatggataat ccggagttat taaaatttaa tattgcttga
    32761 gccgattttt caaatacttc ttgtggtaaa atgcagggac ccgcactata gttgtgtttt
    32821 ttcatgagtg tagtttaatt tcgaaattga actgcaaatt tcagaaattc acaccgaaaa
    32881 gacttattat taaaagttgt ttttattata ggttattaac aataacgttt tagttgataa
    32941 gtaataaaat tactttttta tgattttttg tgtttcataa gaaccatttt caaaacttat
    33001 tttaaataaa taaattcctg attggaagtg atctagagaa agagtagaaa tctggtttga
    33061 aacagaaagt aataatttcc cggtgataga aaaaagttca tatcgctcga cctgatttga
    33121 agtttggttt tcaatcgtaa tactattatt tgttggattt ggataaatag aaattacatt
    33181 tttattaaaa gtagggatgc ttaaactttc taaattgtac gaataggctt taccttgatt
    33241 aataattcct tcacgatttg caccaagaat taaatttgat ccttgtaatg aaatgctatt
    33301 acctaaaaaa tcatccatgt tagattgtcc ttcgccatag aagtaagttt gcatttgcca
    33361 ttcgtattca atttttttat aataaaaaac agggaatttt cgacttaatt gcaagaaata
    33421 aaactcatat ccaaccaaca tattatttcc ggaaacagca atttttccaa ttacaaaatc
    33481 atcaaaattc attggtaata tactttcatt tacccaagaa ttatttaccc gatttaaaat
    33541 gtaaacttta tttaaaaact ggctgctcat ctgtatggca gttatgtata atttttgtcc
    33601 gtcaaaggaa aaatctttta tattttcttc caaatttcca aattctaaag ttgaatcatt
    33661 gtatatccag tttgtttcaa aaataaaagt atgatattta gtattttgtt catttaatct
    33721 atctgaaaca actaaatagt ttccttcaat ttcaattttt tcacctaatt ttatactgtt
    33781 tgcaatggtt aatttttgat taaatatcca ttccgtttcg ttaaaagtat aaacatagac
    33841 tgagccgatt cctattgaat ttgcatcatt tggtgcggaa acaaataatg aattcccttg
    33901 tatttttaca aaagagccaa attgtttgtt tgcagaggca tctgaagctg taattttttg
    33961 aaaaaactcc caattattat ttactttttt atataaataa accgcaccgg aatcagaaaa
    34021 cccttcatca tgtaatggtg acccaacggc aataaaatct ccaaaaacag ctactgattt
    34081 tccaaataga tcatttgata aagcatcgct cggataaaag taagcttctt gtgttatgga
    34141 ttgattagtt tgattaaaaa cataaacata gcctgttgtc aaaggtggaa actgattggt
    34201 agcacttgag accactattg tattttcgtg tatggcagaa gcagatccga atcgtaaacc
    34261 agaaatagtt gaactaggat aaaatccact taaattggtt tgagcaaata aaaagttact
    34321 tacaaaataa aaggatatta atatagtttt tgttttcata taaaatattt attgcactaa
    34381 gttaagtaaa attactacac cgaaatcaag aattgtaagg tatccacatt gtcagcataa
    34441 tcccaaagtt ttggtttttg cgtttgaccg aaagctatgc tgttatctat taaatgattc
    34501 gatacaatac attgaatttg gtcaccttca ttttttaaac gatttttcaa tatttccaaa
    34561 tcttcataaa attcataaaa tacactggaa ataggggagg aatagctgga atcttctttt
    34621 aaagttaaaa acccattatc caataatttg aaattactca tcagaaaaac tgccttattg
    34681 taatcataat tatttgcgta tttttcataa tgaatcacgt cttgatactc aaaaattgcc
    34741 ttgaaaaagg agtcaaaatt atatccattt ggcacaaata ttttcgaaac attccgacag
    34801 cctaacccaa aataacggaa aatatcttct cctaaattga ccaaatcttc ctgcgtttca
    34861 ttcccggtta aaatagctac ggaattcctg tttttacgaa taatggaagg cttattttta
    34921 aaataatatt caaaataacg ggcggtattg ttgcttccgg ttgcaatgac tgcatcaaaa
    34981 ttttcgagtt ttccatcggt aaaagtgatt ctttcttcta aatctttatc aacagaaatt
    35041 aaatagtttg caaaaaattt aatcaaatgt tggtcgttag aagaagtttt aatcaaaaca
    35101 gtatgtccgg taatcaaaac cgataaaaaa tcatgaaatc caacaagcgg aatgttaccg
    35161 gctaaaatta atcccacttt tttgggtgta actttcgaaa aatcatagtt caaaagccat
    35221 tcatttaagt tagtttctgt taaagcttct acccatgaat tgacagaaaa atacacttgt
    35281 tctttggtaa accatccatt atgtgattgt gaaagttcta atattgcttc gaaagaatca
    35341 aaaaaggcat cattatttaa aactccttgt tttctaaggt tagaatctaa tgaaaactga
    35401 cttaaaaaat cacctaaagc aacaaaagaa tttattttgg ttttttgtaa catttgtaac
    35461 ttgtttatat atggaattga ttgtaatttt gcacaaaaat aaatcaaaaa ttagctatgg
    35521 caatcataat aactgacgaa tgtattaatt gcggtgcttg cgaaccggaa tgtccaaata
    35581 ctgctatcta tgaaggtgca gacgattgga gatataaaga tggaacaaaa ttaaagggaa
    35641 aagttatttt accggatggt actgaagtag atgcagatgc tgctcaaaca cctatatcgg
    35701 atgatattta ttatatagtt cccggaaaat gtacagagtg taaaggattt catgatgaac
    35761 cgcaatgtgc cgctgtatgc ccggttgatt gttgtgttcc ggatgataac catgtagaaa
    35821 gtgaagaaac gttgctgaat agacaatcat ttttgcataa tgagtaataa tttgaatccc
    35881 ttgaatgagg gattttttta tatgaagaaa atatttctag tactattttt tttatttcac
    35941 tttaattttc ttttttcaca atcgattaaa ggaaaagtgg ttgatgaaaa caattctcct
    36001 ctatatggtg tttctgttta ttttgatggc acaacaattg gaaccaccac aaataatgaa
    36061 ggagtttttg aattttctgt gaatcaaatt ccaaatgtga attttgttat tagctatatt
    36121 ggctatgaaa cagtttatat gttgtctatt caatcaccaa taaacattaa acttaatccg
    36181 ttagaaattt cgcttaaaga agtggtttta gaacccattc cattttccag aaaagaaatg
    36241 ttgacagttt ttagagaaca ttttttagga agaaccaagg gaggaaaaaa ctctgtaatt
    36301 ctaaacgaag aggcaattca gttctcttat actgccaaag agtttgaatt agctgcgttt
    36361 gccgatgaaa aaataagagt tagaaataat tttttaggct atattattga agttgattta
    36421 atcgattttt atattttata caacaaaaaa acattatcaa ataacttttt gagtggtagt
    36481 tactttgctg taacctcgtt ttataaagaa ataaacggag taacaaaatc atttgaaaag
    36541 aacagaattt cctcttattt aggatcatcc aaacattttt ttaaaaattt aatagataaa
    36601 aaatggggaa agaaagaatt tgttttgtat gatggaagtt ttgctaccga tgccgatttt
    36661 cattttgaaa ttttagatgc aaaagacatg aaattaatta gagtaaaaga aaaacaaatc
    36721 acaactaatt tgttatcaac tcaaaaattc tttagaagtt ttaacatgtt gtataataac
    36781 aaagaacaat ccggaattat ttttaaaacc aatgaattct atgttgatat atttggcaat
    36841 cactctaata tagatcaaat tgatttttcg ggcgaaattt caaaaaagag gtttggcgat
    36901 atgttaccat tggattttgt actaaaataa aaaaaatcct gagttttctc tcaggatttt
    36961 ttttgaatta aatttattgt ttgactaaaa tataagtttc aaaggttact ttatctgttt
    37021 ttggattata tgtttctaaa actaaatttc caattgaatc aaaaaaacca atagatgttt
    37081 tatttttact gctataaata tagctgttag ttgatgtttt gtaaatatta cccgtggcta
    37141 catttgtttc atttaagatt gaataaccat tcggttctga agatactttg tatttaacac
    37201 catctaataa ataataagaa gaacggtcaa tatctacagt tcttatagtt ccatccggat
    37261 ttataatttc agttgttttt gtagcaatta tgggagaatc tttttgttct acatttattg
    37321 tattcgatgt aggagcctga tgttctaata attgaacttc tttaatattt tcatttttta
    37381 tcgtttgttt ttccccttga ccgttatcta ccgttgtaat agtagttttt gtttcttctt
    37441 taatattttt attttgagaa tagccaaaag aaaaggtcat gattaatacg atgattgcaa
    37501 ctaatttcat gatttaaatt tttaataatt aatagggaat aaagatatag tttacaatta
    37561 gttatcttgt tgcataatta attgcaatgt ttgcaggatt cacatctttt tgatttgatt
    37621 ctaatataaa tcaattcaag aagctctgtt ttatactttc aattaataaa actatatttg
    37681 cacccgttta aaaaccgttt acccataact cattacccta aaagatgaaa gcaggaattg
    37741 taggattacc aaatgttgga aaatcaacgt tatttaattg tttatcaaat gcaaaagctc
    37801 aaagtgctaa ctttcctttt tgtactatag aacctaacat tggagtagta aacgttcccg
    37861 atcctcgaat tactcgttta gaggaattag taaaaccgga acgtgttcaa atggcaactg
    37921 ttgacattgt tgatattgcc ggattggtaa aaggagccag taaaggcgaa ggtttaggaa
    37981 atcaattttt aggaaacatc agagaatgta acgctattat tcacgtactc cgttgttttg
    38041 acaatgataa tattgtgcat gtggacggta acgtaaaccc aattcgcgac aaagaaacca
    38101 tcgacatcga attacaatta aaagatttag aaaccgttga aaagcgtttg gaaaaaacca
    38161 atcgtgcagc taaaaccggt aataaagaag ccattgtaga aaaagcgttg ttagacagaa
    38221 taagagaagc tttgctccaa gcaaaatcgg ctcgtacggt tattcctcaa aatcaagatg
    38281 aaattgattt gatggaagaa tttcaattaa tcaccaccaa acctgtttta tatgtttgta
    38341 atgtggacga agcatctgct gtaaacggaa ataaatatgt tgatcaagtg cgagaattag
    38401 taaaagatga acaagcagaa gtaattgtgc tttctgtagg agcagaagcc gatattactg
    38461 aattagaaag ttatgaagaa cgtcaagttt tcttggaaga catgggatta acagaaccgg
    38521 gatcagctgt tttaatacgt gccgcttata aattattaaa attacaaact tattttaccg
    38581 caggagtgaa agaggttcgt gcttggacaa ttaatgttgg cgatactgct ccaaaagcag
    38641 ccggggttat ccatactgat tttgagaaag gatttattcg tgcagaagtt attgcttttg
    38701 aagatttttc aaactatggt tcagaagcca aggtaaaaga agccggtaaa ttacgagtag
    38761 aagggaaaga gtatattgtt aaagacggtg atgtgatgca tttccgtttt aatgtataaa
    38821 tagtattatt tttagttaaa ttaacctgca aaatttattt tgcaggtttt ttgtttttaa
    38881 taaatacttt tcattccttt atacacctta atcctactat a
//
LOCUS       DAOTCW010000006        38159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_522_length_38159_cov_45.808052, whole genome shotgun sequence.
ACCESSION   DAOTCW010000006 DAOTCW010000000
VERSION     DAOTCW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 38159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38159
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_522_length_38159_cov_45.808052"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..378)
                     /locus_tag="P5335_01020"
     CDS             complement(91..378)
                     /locus_tag="P5335_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRZ73491.1"
                     /translation="MSHFVYILFSKTTSKYYIGETHNVDERVKNHNQHNYLNSYSKIA
                     NDWELAFCFECSDRNNALYLEKFIKKMKSRLFIQKIIANPEILSDILSKKK"
     gene            complement(428..500)
                     /locus_tag="P5335_01025"
     tRNA            complement(428..500)
                     /locus_tag="P5335_01025"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(465..467),aa:Phe,seq:gaa)
     gene            601..1335
                     /locus_tag="P5335_01030"
     CDS             601..1335
                     /locus_tag="P5335_01030"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRZ73492.1"
                     /translation="MLLAIDVGNTRIKGAVYERNTLINQFVFSSVEVKNNIENILEKN
                     PKITDLIVSSVGNENSIVFNSFSERISIHFVSHQDIFPFQNKYATPTTLGIDRMVLAS
                     GATLLFPKKNKLVIDAGTCVTYDFIDDENRYWGGAISPGLRLRYESLHQFTAKLPLLT
                     LEEPLNFIGNSTNQSIHSGVVNGLIHEIDGFIEQYKNKNENFTIILTGGDAEFLAKRL
                     KNTIFANSNFLLESLNQFFQYKINND"
     gene            1358..2581
                     /locus_tag="P5335_01035"
     CDS             1358..2581
                     /locus_tag="P5335_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73493.1"
                     /translation="MIFSFFSNAQNASSSPYSFYGIGDLVFKGNVETRSMGGINMIND
                     SIHININNSASLSDIMNTTFTIGGNLNYVNYKTASQSETANRASLDYIALAVPISKRL
                     GAAFAVMSFTSVGYQIQNNYVDDSTPISRFTGSGGTNKIYGGVGYKINKNFRIGADMN
                     FNFGNITTNDISFLTGIQYGTRELNSSEITGFNFNTSLMYNKLINKTTTIFGSLIYSP
                     ESTLSLANERKIAIVQFSESFGELVVNEEDVPVNDTKVKYPSKIGIGFGVGEVRKWSV
                     GAEVTFMQGSNFGSRFDDINDVSYENVTKYSFGGYYMPDYNSFSSYYKRITYRGGFNY
                     QNTGLIVNNESINNFGITFGLGLPIKGSFSNVNVGFEFGKRGTTSSGLIQENYSNLSV
                     SFSLNDKWFIKRKYD"
     gene            2601..3959
                     /locus_tag="P5335_01040"
     CDS             2601..3959
                     /locus_tag="P5335_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRZ73494.1"
                     /translation="MKTKVIILFAFILVTFNGFAQSAANCAEKLSIFAEMAKTKNYGE
                     ETYKHLQELRKDCPSFSEAIYIYGERIINSRIDNAKTPADKEKEVRDFIKLQDEFKTN
                     FPAKAKGIEVKKAMVLFENNSGTSDEVYQLLDTAFKNDKDNFSNPKALYVYFEIYVND
                     FEANKNGIKLQDIFNKYDQVSDKIDEEEKGVSDALDVLLQKEESGTELTDKEKRAKEN
                     HQINLDAFETIHSSMDAKIAQLATCERLIPFLGASFEENKANAEWLKRAADRLDKKGC
                     SSDPLFSKISDALHSLNPTAESAYNLGVSFYTKKNTAKALEYFNQSAELQKDNTKKAN
                     VYYTIARNIYGNGNKVQARAYAEKALAAKPSFGEAHVYIAQLYANSANDCGNDPFEKR
                     AIYWLAAQTARKAGTASGNAAASSYDKLAPSKQEIFNSGRAGQSICFKCWVGKCVTVP
                     NL"
     gene            3964..4518
                     /gene="lptC"
                     /locus_tag="P5335_01045"
     CDS             3964..4518
                     /gene="lptC"
                     /locus_tag="P5335_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRZ73495.1"
                     /translation="MLSIKKIINFSVTVFAVTLFFACESNFKEIQKINISEFMASGEA
                     DSVNLKYTDSGRIKAILISPKMFDYGTVQYPFTEFPKGIDVTLFDEKGKKNYIKADYA
                     ITFKGTDIIDLQGNVKMTADGGQFLETEQLYYDQKNEWFFTEKKFKFTDPNKGLSTGE
                     GLDFSKDFKRINFQKVYGLINDAE"
     gene            4759..6024
                     /locus_tag="P5335_01050"
     CDS             4759..6024
                     /locus_tag="P5335_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRZ73496.1"
                     /translation="MEVSIIIICLLLSAFFSGMEIAFVSSNKVYLGIEKLQEGFVAKI
                     LTKITEKPSMFIASMLVGNNIALVVYGFMMGDLIIKLLFPDYLEIHNLPFQILMIQTL
                     ISTAVILITAEFLPKVFFQIYANNCMKIFAVPAYFFYELLYFISAFTIWISDFVLKKV
                     FKTQGDETQLFFSKVELGNFISEQMNSVEDHESVDSEILMFQNALEFSGVKARDIMIP
                     RTEISAVEIHDSVSEIKQLFIETGYSKILVFQSSLDDIIGYVHSFELFRKPKTIKSVM
                     ISVEFVPETMFIKDVLDLLTKKRKSVAIVLDEYGGTSGMITVEDIVEELFGEIEDEHD
                     KDETLVEEQINEKQYRFSTRLDVEYINETYKLNIPEEDSYSTLGGFIINHTKEFPSKG
                     EKISVENFHFVIEEASNRKIELVTLTITN"
     gene            6213..8315
                     /locus_tag="P5335_01055"
     CDS             6213..8315
                     /locus_tag="P5335_01055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRZ73497.1"
                     /translation="MAILGKIRQRSFILIVIIALALFSFVLADVIQSGGFAQTSTNVG
                     SINGKDIAFEEFRLKVGNTEKGGQGMSTIQAVNRVWDQEVNVALLTEELEKLGIRISE
                     KHLIETLKNDPNFGQNPSFQNELKQFDINKYREYIKASSNPQEVEYFESTEKNANINS
                     KYQIYSSLVKGGFFTTQNDAKFKYNLQNNKVTFDFVAVPFSSIKDSDVKVTDEEIIEY
                     MKKNEKKYKAEETREIEYVVIEDKPSDADKTEIKNKVASLLNSKVVYNNETSTNDTIA
                     GFSNTKNVIEFVNANSDFPYDSTYVGKSDLPAEHAEQIFNLPEGQIYGPYVYGEYYAL
                     SRSMGRKAGAKARASHILISFTGSRAQPKEDRTKEQAKVKAEELLGQINANPDSFTML
                     AFTASEDQGSAQKGGDLDFFGPGQMVPAFNDFVFNNPIGKVGLVETEFGYHIIKVTDK
                     QDAIRLATIAQKIEASEKTAEVAYTNAVKFELAAAEKSFEAAVKEAKLTVVPPVRVKA
                     LDETLGQMGAQRQVIRWAFESDTELNDVKRFEIAKVGHLIAKLKKVNEEGLLNIEDAR
                     LQVEPILKNKKKAEKIMAKMKGATLEAMATANAVQVQNAADVTLENPVVATLGYEPKV
                     VGFAFSTPANKLSAPIEGNASVFVVKTTKVTKALPTQDYSAQLNALKSQNASGVNRVF
                     SALKDKAEIKDNRFQFNY"
     gene            complement(8407..9324)
                     /locus_tag="P5335_01060"
     CDS             complement(8407..9324)
                     /locus_tag="P5335_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HRZ73498.1"
                     /translation="MKKTFYSNGKLLLTGEYVVLDGALALALPTKFGQNLLVKPGKTN
                     EINWKSIDADGSTWFEEVFTFDSIVQENKTMSENPIHKTLIEILHEAFQMKPDFLLGN
                     NGYSIETNLSFPKNWGLGTSSTLINNIGQWLELDAFELLQRSFGGSGYDIACAKKDNP
                     IIYQLINNQPSYKKVAFYPEFRNHLYFVYLNRKQSSKSAIANYINKQNEIGKTIEKIN
                     AITKSILSTSDLKTFAYEIEKHESILSEVLEMNTIKEAFFDDFKGVIKSLGAWGGDFV
                     LAISKENPSGYFKERGFDTVICYDDMILK"
     gene            complement(9332..10648)
                     /locus_tag="P5335_01065"
     CDS             complement(9332..10648)
                     /locus_tag="P5335_01065"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HRZ73499.1"
                     /translation="MNHEVSGFSKLSKIEKIDWIAQNYFSEPKVAVALLQNYWNSDEK
                     LQQLHDEFIENTISNFYLPLGIAPNFNINGRNYTVPMVIEESSVVAAAAKSAKFWSKR
                     GGFKTTVLNTEKIGQVHFIYKGETSKLVAFFNQTKAKFYSETESITKNMQKRGGGILD
                     IVLKDKTSDLENYFQLHATFETKDSMGANFINSCLEQFAKTLKAEAQNYTAFSEEEKN
                     IEVVMSILSNYVTNCIVRAEVSCPIEELAEKHIENPTEFAEKFVRAVQIATIEPYRAV
                     THNKGIMNGVDAVVLATGNDFRAVEAGAHAYASRTGKYSSLSYAKIEKGIFSFWLEIP
                     LALGTVGGLTSLHPLVKLNLEILEKPSAKELMQVVAAVGLAQNFAALRSLTTKGIQEG
                     HMKMHLNNILNQYHATAEERIQVLAYFKDNTISHASVSEFMEHLRK"
     gene            complement(10703..10984)
                     /locus_tag="P5335_01070"
     CDS             complement(10703..10984)
                     /locus_tag="P5335_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRZ73500.1"
                     /translation="MKIRFSEKFINDLSEQVVFIAKDKPKAARKFKNDLLRYISKDLK
                     TPFNFRKSIYFDDELIRDYIFKGYTITFKVNLQKKEIIVFGFIKYKNSL"
     gene            complement(10974..11207)
                     /locus_tag="P5335_01075"
     CDS             complement(10974..11207)
                     /locus_tag="P5335_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73501.1"
                     /translation="METIRIQFQPNLKSKILELLSSFSENEVQIIQEDPSFEKNKRIL
                     EEELKNIESGKSKCVSMEELDLVMEKIISTYED"
     gene            11371..13545
                     /locus_tag="P5335_01080"
     CDS             11371..13545
                     /locus_tag="P5335_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRZ73502.1"
                     /translation="MNHFNKVIFLFLFISFAAIGQQKITLEDIWASGAFRTKGMDALQ
                     AMKKTNQYTVLNFDRASKTFQIDLYDFATLKKVSTLVDSKNHVELSSIDSYTFNEAET
                     QLLIALNSEQIYRHSSVADFYIYDIATKTLQKVADYKIQEPLFSPDGKKIAYGYQNDL
                     FVLDVASKKHTQITNDGKKNEIINGITDWVYEEEFAFVRAFDWNKSGTHLSYIKFDES
                     NVPEFSMDVFGKELYPSQEVFKYPKAGEKNALISLHIYDVASNATNKINLSQYSDFYI
                     ARMKWTNDANALSVQVLNRHQNNLDLLFVEAKTGTTKVVLNEKDTAYVEVTDNLTFLQ
                     DNSFIWTSEKDGFNHIYHYDKSGKLRNQITKGNWEVTSYYGFDEKNGQVYYQSVENGS
                     INRDVYKIKLDGKSNVRLTNKTGTNKATFSPNFQYFINSFSSSTEPTSFTLHNSKDGK
                     QVQSIVDNNELASKLKSYNLPTKEFFELTTEKGHKLNAYIIKPKDFDASKKYPLLLFQ
                     YSGPGSQQVANQWLGANDYWHMMLAQQGYLVACVDGRGTGFKGAAFKKVTQKELGKYE
                     VEDQIDAAKVLGNYTYVDKTRIGIWGWSYGGFMSSNCILKGNDVFKMAIAVAPVTNWR
                     FYDSIYTERYMQTPQENASGYDDNSPINHVDKLKGNYLIIHGTADDNVHFQNATRMIR
                     ALQLANKQFDQAIYPDTNHGIYGGKIRLQLYTKMTNYINQNL"
     gene            13564..15240
                     /locus_tag="P5335_01085"
     CDS             13564..15240
                     /locus_tag="P5335_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRZ73503.1"
                     /translation="MSEVAVKSGHPKGLWVLFGTEMWERFNFYGMRAILTLFMVNALM
                     KNEAEASLIYGGFLGLCYLTPMLGGFIADRFFGNRNCILLGGLMMAVGQFLLFLSASV
                     FGTNLSLADTLLWSALGIIIFGNGFFKPNISSMVGSLYPKQEKSKLDTAFTIFYMGIN
                     MGAFLGQLICPIVGDVKDVDGIRDIHAFKYGFLAAAIAMLIGTAIFYFLKNKYVVTPE
                     GRPIGGLPKHNVNDDYEEGESQKAIFSQTALIGAVVGFIGLGLVFHYVFDQNIIYSII
                     YASGISLAGLIISDSSLTKVERDRILVIYIVAFFVIFFWAAFEQAGSSLTFIADNQTD
                     RNFFGYNMPPSMVQIFNGLFVVIFAVPFSLLWDKLREHGKEPISPVKQAVGLALIALS
                     YFIIAYNVKDLGNDGLLAIKWLILLYLIQTCAELCLSPIGLSLVGKLSPKRFASLLYG
                     VFFLSNAAGYALAGTLGSILPATGEKFNKAKDMGIDLQAVLDKTVTPTAEQLKLLADN
                     QISPTNPVFAGFEINNLFEFFMVFVILCGLAAVILMALTPKLKKMMHGVR"
     gene            15318..16976
                     /locus_tag="P5335_01090"
     CDS             15318..16976
                     /locus_tag="P5335_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRZ73504.1"
                     /translation="MWKSHPKALPYLFLSEMWERFGYYLMIGIFTLYLKDVEAGFAMT
                     EKEASDLYGTFIALVFLTPFIGGLIADRYWGYKKSIIIGGLMMGAGYFMMGIHNITVL
                     YIAMTLVIVGNGFFKPNISTLLGNFYNEDKFKEKKDEGYNIFYMGINIGAFICNFFGA
                     ALKILFGWQYAFMAAGVGMFIGVIVFILGTKHYGNKTEKKGVQEGDMPFYKIVLFILL
                     PSALFGIIGWFINGVQSDANPNCSIFGSDSTDAFIFACIPVIFFYASLYFKAKKEDKR
                     PIGALLTIFGVVILFWAVFKLNGSALNNWGDKYTDRELTGVSQTIASKLVLTDTLVYK
                     RDSVALYDEAFRIQKKDGKVIKVVDYPLYFRNVKKEKLPEENSKIFTWSANLSQSINP
                     GWVILLTPLVVAFFTFLRGRKKEPSTPTKIAFGLLISALSVLVMVAAVNMGGNGSEKV
                     SVWWLVANYGVITIGELFLSPMGLSVVSKLSPTNITSLMMGGWFLSTSIGNKLSGVLA
                     SLWDTYDNKADFFWINFGLLMFATLLMFVLLKQLNKVMKEKGIN"
     gene            16987..18489
                     /locus_tag="P5335_01095"
     CDS             16987..18489
                     /locus_tag="P5335_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRZ73505.1"
                     /translation="MYTVEEIHDFKGKYPKQLWYLFFSEMWERFSFYGMRGMLAVFMV
                     SQLMMDEKTANLQYGATQAFVYAFTFIGGLFADKILGYRKSLFWGGLLMIVGSIILAI
                     DPKQFFFFGISFTIIGTGFFKPNISTMVGKLYRDGDNRRDAGFSFFYMGVNLGALLGG
                     YICIAVANGTLWESFIPEHLRWNIAFGFAAVVMIVSLLTFTQTQKTLGEIGLSPLAHL
                     EKSKRRTLEIATYIGSLVIIPIIMTMVAKTEYTDYFMFIIGPLALLYLFFEMRNFSLV
                     ENKKLLAALVFILFSIFFWAFFEQSGGSLSLFAANNLDNTVLGVKLDPNGVNNSSNSF
                     FVIAFAALVGMAWLWMNKRKIEPNTVVKFGLAFLFLAGGFYVFYYTKFFSNPDGITSL
                     DLFTMGWLVITFGELCLSPIGMSAMTKLSPQKTQAVIMGMWFLASAYGQYFAGILGAN
                     IAEASENSSNFDKLIVYADGYKDLAIYALVFGLVLIAISPFVKKLMQDVK"
     gene            18541..19005
                     /locus_tag="P5335_01100"
     CDS             18541..19005
                     /locus_tag="P5335_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HRZ73506.1"
                     /translation="MKKLLFALFLIITTVSSQAQEIKWMTLDEALALQKKNPKPIFMD
                     VYTDWCGPCKMLDKNTFQTKEVSEFINATYYAVKFNGEGNSVVNYKGKKYTNPGYDAT
                     RKGRNSMHEFTGFLNVEGYPSMYILDKNGDIAKVVVGYYQPEQLLAELKTVK"
     gene            19006..19461
                     /locus_tag="P5335_01105"
     CDS             19006..19461
                     /locus_tag="P5335_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRZ73507.1"
                     /translation="MNKIILYFVFFWSLQTLSAQELKWQTDINKAIELSTKSKKPLML
                     FFTGSDWCGWCIKLQKEVFQKPEFVEWSKKVILVELDFPKKTQLEASLKQQNDQLQQI
                     FQVQGFPTIWFVSPQKNGTQINLQQLGKTGYVAGGPEKWLEAVNQFMPK"
     gene            complement(19463..21478)
                     /locus_tag="P5335_01110"
     CDS             complement(19463..21478)
                     /locus_tag="P5335_01110"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRZ73508.1"
                     /translation="MKFPTLNITLCVVLGILASNEVKPDINSACLLVSLLFIPFLLFF
                     WLSNKKAIFRFYFGLSAYLLSFFVGFMVYSLHFKHNQPLHYSNELKNEKSLVGGVISE
                     KIRPNKFTHKYFLEVETVDKIDKTGKLLILLSKKDAKKEFQIGDVILAYAFVKPIIRP
                     HNPYQFDYAAHLEKKDVFHQLYLEDNNYSVVSCEKNLYYKLEQIRNVIVTKLENANIS
                     SNRLEIIKALFLGQRQDIDQETNNQYSQAGAIHILAISGLHIGILLFFFKTLLKPLER
                     FRYGKIILPFLLIFILWSFALLSGLSASVVRAVTMFSFITIGMYMRRETNIYNTLGSS
                     ILLILLIRPNFLFDVGFQLSYCAVFSIVTIQPLFAKLWKSSNKIIQYFYDILTVSFAA
                     QIGVLPLSLYYFHQFPGLFFLTNLVVIPLLTLILVCGMIALLFAVMRIPTEIFIHLLS
                     FLVGLMNRYVAFISSLEKFIIKGIPFNLTLLFSSLLALIGIVLWFKKPKFNRILIALI
                     FILLFQLSIIGSLEYHKSNDEFIIFQYPKQTILAKKTNNSLTIFSNEKDIADNYVLKN
                     YLQANFGQIGSVTKLKNILQINDTKILVIDKGGIYSIPIKPNIVLLTQSPKINLERLI
                     GELNPAIIIADGSNYKYLVENWQQTCLQKNIPFHSTSEKGYYKIELN"
     gene            complement(21534..22196)
                     /locus_tag="P5335_01115"
     CDS             complement(21534..22196)
                     /locus_tag="P5335_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRZ73509.1"
                     /translation="MKNTVLITFLLLFLVSCKPTTSVIVTSKEEAQKKGIYKAPQPNK
                     ATVSTNKSAGSSSVKKGTENTKSEKKHKITDVEDTDFVADNDNTSYFIKQLINTAMQY
                     NGVRYRGGGTTTAGMDCSGLVTTVFKTYDVSLPRSSNDMSKVGEKLNRNEIKKGDLVF
                     FRTNGRSVINHVGLVTEVKDDEIIFIHSSVQRGVIISSTKEPYYQRTFAQANRILGDE
                     YF"
     gene            complement(22232..23350)
                     /gene="lpxB"
                     /locus_tag="P5335_01120"
     CDS             complement(22232..23350)
                     /gene="lpxB"
                     /locus_tag="P5335_01120"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRZ73510.1"
                     /translation="MKYYIIAGEASGDLHGSNLMKALYKKDPKAIIRFWGGDLMQKTG
                     GILVKHYRDLAFMGFAEVLFNLKTIVNNISFCKKDIEQFQPDVLIFIDYPGFNMRIAE
                     WAKKKGIPTHYYISPQIWAWKENRIKAVKRDFDKLYVILPFEKDFYEKKHHFPVEFVG
                     HPLIDAIHNRVKIENETFRKENNLDERPIIALLPGSRKQEISKLLHEMLAIVPDFPDY
                     QFVIAGAPGQEFDFYKQFLTNKAVHFITNKTYDLLSVAHAALVTSGTATLETALFKVP
                     EVVCYKGNWASYQIAKRIITLKYISLVNLIMDEEVVTELIQSECNSKRIKAELTKILN
                     SEYRQKLLKKYEILEEKLGGEGASEKVAQLIVASIARF"
     gene            complement(23354..23626)
                     /locus_tag="P5335_01125"
     CDS             complement(23354..23626)
                     /locus_tag="P5335_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73511.1"
                     /translation="MQKRDLFIGFLLGIIGAILGVFLFITFFTSYDFLEGITILKSQN
                     SLGKLIALGAVINVALFFLLLKFQKDVMARGVVLATIALTIATLFV"
     gene            complement(23626..24399)
                     /gene="surE"
                     /locus_tag="P5335_01130"
     CDS             complement(23626..24399)
                     /gene="surE"
                     /locus_tag="P5335_01130"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRZ73512.1"
                     /translation="MKKPLILVTNDDGISAPGIRALIDVMKTLGDVVVVAPDSPQSAM
                     GHAITINNTIFVNKVHFDSELKGEYSSSGTPVDCVKLAVNEILKQKPDLCVSGINHGS
                     NSSINVIYSGTMSAAVEAGIEGIPAIGFSLLDYDWNADFEPIKKAIRTITSEVLANGL
                     PDGVILNVNFPKLKEEEIKGMKVCRQAKALWMEKFDKRKTPQGKDYYWLTGEFVNQDK
                     GEDTDEWALEQGYISIVPVQFDLTAHHAIQKLNTYKFEL"
     gene            24639..26864
                     /locus_tag="P5335_01135"
     CDS             24639..26864
                     /locus_tag="P5335_01135"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HRZ73513.1"
                     /translation="MNTILLFMKRNFKLVLLAIVLSASLLAFKATENNISSSADPEKD
                     KLLLELLTFVIERGHYSPVDIDDNFSKGVYKDYVEALDPSKRFFIQSDIDEFAEYETK
                     IDDQIKNKDLTFFELTYSVYLKRMNESQKYYKEILEEPFDYTVDETINTEYEKLPYAK
                     NSSELKERWRKQIKLSTLSSLTDKMKLEEDKLKGTDATNNSEKKENKAKVDAKPKSFE
                     ELEKETRESSLKSLDDYYTFIKELDRNDWFGVFVNAIASRFDPHTNYMAPDDKERFDV
                     SISGKFEGIGARLQKKNDLTEITELISGGPAWRGKELEPGDIVLKVAQGDQEPVDIVG
                     MRLDNVVKKIKGPKGTEVRLTVKKVDGSIKVISLIRDMVETEETYAKSSVVERNGMKY
                     GIIYLPKFYIDFENNDGRDAGKDVAIEVERLKAQGVKGIIMDVRDNGGGSLKTVVDIT
                     GLFIEQGPVVQIKSAGTKKEVLFDRDAKIQWDGPLVVMVNNFSASASEILAAAIQDYK
                     RGVVIGSKQTYGKGTVQNVIDLNQFVRGNTLGDLGALKTTTQKFYRINGGSTQLEGVS
                     SDVVMPDRYSYIDIGERDLDNAMPWSKIDEAQYSVWNKQTNFEKAIENSKNRMAGDEH
                     FQLIDKSAKWTKVRRDAVVYSLKLDAFLAEQKAVEEQGKVFKPITDYKNDLLFKAIPE
                     DEVVMKKDESFKEKRERWFESLSKDIYVEEALNVLDDLQTKPVVKGKVELKKKEKVVK
                     S"
     gene            26909..28015
                     /locus_tag="P5335_01140"
     CDS             26909..28015
                     /locus_tag="P5335_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRZ73514.1"
                     /translation="MSNTNQSLTAIALQKFKKNFWGVFSLSYIVLMIFLALFAYVIAP
                     DNSENANQMHLSIHSKSPGFAVQMLSIPSSKIEDKTIKGYFFGHPNQVTEIPISSYQI
                     KDNKLQYVEYTEDKTLRLVKEITLDKFIDYNSFKQVEQELITNKKFYLGTDKYGRDLL
                     SRMLVGSRVSISIGFVAVLISVVVGLFFGALGGYYGGKIDALVMWLVNIIWSIPTLLL
                     VIAITLALGKGFWQVFVAVGLTMWVEVARVVRGQIISVKEMQYVTAARALGFSNGRII
                     LNHILPNIMAPVIVISAANFASAILVESGLSFLGLGAQPPIPSWGSMIKDHYSYIILG
                     KPYLALVPGIAIMLITLAFMMTGNALRDALDVKS"
     gene            28179..28853
                     /locus_tag="P5335_01145"
     CDS             28179..28853
                     /locus_tag="P5335_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HRZ73515.1"
                     /translation="MDFDFYPTSTTNQIIKHNFYRLSYSEKNEQAEWVAYELKGNTFQ
                     NNFNRPYFVADPLVKTGSADWKNYKNSGYDKGHLCPAGDMKFSENAFEDTFYTSNISP
                     QKPDFNAGVWNRLEQKVRYWADKYDAIYVVTGGVLEPNLKTIGREKVAVPDFFYKVLL
                     DVSKDNPRMIAFLVPHQNSDKPLYEFVIAVDELEKRTGIDFFYNLPDEIENKLEKQTD
                     YKMWSF"
     gene            complement(28862..30115)
                     /gene="rodA"
                     /locus_tag="P5335_01150"
     CDS             complement(28862..30115)
                     /gene="rodA"
                     /locus_tag="P5335_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRZ73516.1"
                     /translation="MKNQSVASNLDWTTVLIYGLLVLMGWLNIFSTSIPIEFTEFDFG
                     DYYGKQLIFILLTIPVIIVVLTIDAKIYEKFSFVFYILGIVLLLGLFVFGKTVKGQTN
                     WYGFGSFGFQPSEFVKTATSLLLAKYLSDSQVFLKNTNHQLLAFAIIGFPVLLILMQP
                     DAGSAMIFFSLFLVLNREGLPEWYLWTGFLAVVLFFAALLIQPIYLIVVIVIIMILHY
                     LRARRINRIPLAYLIVFVAATSFVLSVDFVFENVLEPHQKDRINVLFSDTVDLKNEGY
                     NLNQSMIAIGSGGVIGKGFMEGTQTKGGFVPEQHTDYIFTTVGEEWGFIGCTVVILLF
                     VTLLFRIIYLAERQKTKFSRVYGYCVASILFTHIFVNITMLIKLFPTIGVPLPFFSYG
                     GSSLWAFTILLFVFLKLDANKVNEW"
     gene            complement(30115..31968)
                     /gene="mrdA"
                     /locus_tag="P5335_01155"
     CDS             complement(30115..31968)
                     /gene="mrdA"
                     /locus_tag="P5335_01155"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRZ73517.1"
                     /translation="MRKAILPSLIIFATLVVLARLFYLQIIDNEYKLKSENNAIKIVY
                     EYPERGYIYDRKGKLMVANQPSYDIMVVPREIKNLDTLKFCDLLSITREEFDKKIKKA
                     TVYSPRLPSVFLPQLNKKEYAAFQEKIRKFEGFYIQKRSLRDYQANIGANVFGFITQV
                     SPAMIEKNPYYKSGDLIGKQGVEEYYEDFLRGQKGVKYIQRDKFNRDIGPYKEGIFDT
                     LSKQGDDITLSLDMELQKYGELLMQNKRGGIVAIEPKTGEILALISAPTYDPSLLVGR
                     MRSKNYTALYNDSIAKPLYDRGLLAEYPPGSPFKILTALVALQEEVIDEQTTYFCRRG
                     FSYGNRFMKCHDSGPSQLHDGIYKSCNTYFANIYLKTIQKYPNPADGVDVWSNHLKSF
                     GLGNFMGYDLPPGRKGSIPTSKLYNKYYSAGGWKSTTIISNSIGQGEVLMTPIQLCNV
                     MCAVANQGYYYTPHIIKKIKDHTIDKKFTTKHQTTIDKQYFPPVIEGLFDVYNLGTAS
                     RLKVDGIDICGKTGTAENFTKINGQRVQLTDHSIFVAFAPKDNPKIAIAVFVENGYWG
                     ARIAGPISSLMIEKYIKGKISRTDLEKRMLESSLESEYAKVLSGQPFSINK"
     gene            complement(31965..32474)
                     /locus_tag="P5335_01160"
     CDS             complement(31965..32474)
                     /locus_tag="P5335_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRZ73518.1"
                     /translation="MSSAVIVNIIRFILLILAQVAIFNKINFLGFINPYPYILFIILY
                     PVNGNKNGLLLASFLLGITMDMFSNSGGVHAAACVILAYLRPSFFKFSFGLSYEYQTV
                     KINERLTPERFSFILISVLTHHIILFLLEIFRFSFFWNILLRTVLSTIFTLALCILLI
                     YLFKPSKKI"
     gene            complement(32467..33291)
                     /gene="mreC"
                     /locus_tag="P5335_01165"
     CDS             complement(32467..33291)
                     /gene="mreC"
                     /locus_tag="P5335_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRZ73519.1"
                     /translation="MQQLFVYIFKNSTRLLFLLLLVVSLVLTIQSHSFHRSQVISSAN
                     FVTGGVYERINSVNEYLNLKTENEGLANENARLKSLLFNFKDTTKVIDKELLSSFGNY
                     KVFQSKIINNTYNVHENFLTINSGSVHGIQPDMGVMNSKGIIGIVEHTSKNYATVISV
                     LNVKSQINAKIKKSSHFGTLTWNGKSTGYVQLIDVPRLATVKKGDTIVTGAQSKIFPE
                     NINIGTIHKIYTDNKTNYFTIDVKLFNDMTSLGHVYIIENKDRKEVIELEAQNKDE"
     gene            complement(33349..34377)
                     /locus_tag="P5335_01170"
     CDS             complement(33349..34377)
                     /locus_tag="P5335_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRZ73520.1"
                     /translation="MGFFDFMTEDIAIDLGTANTLIIHNDKVVIDSPSIVARDRISGK
                     IIAVGKEANLMQGKTHENIKTIRPLKDGVIADFDASEKMISMFIKSIPALKKRLFTPA
                     LRMVVCIPSGITEVEMRAVKESCERVNGKEVYLIHEPMAAAIGIGVDIMQPKGNMIVD
                     IGGGTTEIAVIALGGIVCDKSVKIAGDVFTNDIVYYMRTQHNLFVGESTAEKIKIEIG
                     AAIEELDTPPDEMSVQGRDLLTGKPKQVDVSYREIAKALDKSIQRIEDAVMETLSQTP
                     PELAADIYNTGIYLAGGGSMLRGLDKRISLKTDLPVYIAEDPLRAVVRGTGIALKNIT
                     RFRSILIK"
     gene            complement(34434..35960)
                     /gene="purH"
                     /locus_tag="P5335_01175"
     CDS             complement(34434..35960)
                     /gene="purH"
                     /locus_tag="P5335_01175"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRZ73521.1"
                     /translation="MNTTKTIQSALISVFDKTGLEPIVKQLHQQNVILYSTGGTEEFI
                     KNLGIPVVPVEEVTSYPSILGGRVKTLHPKVFGGILNRQDNPSDVEEMKAFEIPQLDL
                     VIVDLYPFEKTVASGASETDIIEKIDIGGISLIRAAAKNFKDTVIVASVNEYAYLLAL
                     ISTQNGATTLEQRRLLATKAFHVSSHYDTAIFTYFNTDVTIYKASIADGQVLRYGENP
                     HQKGFFFGDFDSMFTKLHGKELSYNNLLDVDAAVNLILEFKDNDPTFAILKHNNACGI
                     ATRSTMQEAYLAALAGDPTSAFGGVLIANGKIDEATAKEINHLFCEVVIAPAYDDKAL
                     ALLEEKKNRIILVQHDVALPNKQVRTCLNGLLVQDKNNLTDNKQGLKTVTLTTPTDQE
                     IEDLLFASKICKNTKSNTIVFAKNGQLLASGTGQTSRVDALKQAIEKATSFGFDLKGA
                     VMASDAFFPFPDCVEIAHHAGITAVIQPGGSIKDELSISYCNENKIAMVFTGTRHFKH
                     "
     gene            36185..37438
                     /locus_tag="P5335_01180"
     CDS             36185..37438
                     /locus_tag="P5335_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRZ73522.1"
                     /translation="MLLYLRLLKESFSFAMNALRNNKLRTFLSLLGVTIGIFSIIAVL
                     AAVDSLDQKIKADLGDLDKNTVYLMRFSFGPSEIPRWKREQFPDVTFEEFDYLKKSLN
                     GVDKMAFNLFTGNENVKFEERTVSSVRVTPTTEEFEGIDGIKIEHGRFFNESESNSGT
                     AVVILGYEVANGLFDSAEQANGKKIRMYGKRFTVIGVTEKQGFSTFGDSRDNAVFIPM
                     NFIRRLYGENNENITPAILIKPEKGIDMEAFKGEMTQKLRNYRGLKSSEIDNFFINVL
                     SGFTDMIDNIVGQMNVVGWIISGFSLLVGGFGIANIMFVSVKERTNLIGIQKSLGAKN
                     RFILFQFLFEAVILSVIGGIIGLVMVWLIAMIMTSVLDFEFILSFGNMMLGAGLAGII
                     GLISGIIPAISASKLDPVEAIRSGM"
     gene            37642..38139
                     /locus_tag="P5335_01185"
     CDS             37642..38139
                     /locus_tag="P5335_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRZ73523.1"
                     /translation="MIKDNSNQTFLKYWKRIIITLPTIRDGLKHTNYNSDIRKYTKET
                     DPITQLLKLKILPPTDGKPLEYKYKYNGKEWQDELGLDVYDYGGRVYDPAAPRFWQID
                     PMAEDREWLSPYNYVQNNPIIRIDPDGLLDDLAVGDTDPPTKKADNTSRLSGWCKSSA
                     KSVLI"
BASE COUNT        12898 a         6058 c         6786 g        12417 t
ORIGIN      
        1 tattaaataa caaaaaaccc gaatattgct attcgggttt aagtggtacc tccagtcccg
       61 aagcttcggg agaaccaggg acacatggat ttattttttt ttggaaagta tatcagatag
      121 aatttcagga tttgcaatta tcttttgaat aaataatctg cttttcattt ttttgataaa
      181 tttttcaaga tataaagcgt tatttctatc actacattca aaacaaaatg ccaattccca
      241 atcatttgca atttttgaat atgaatttag atagttatgt tggttatggt ttttaactct
      301 ttcgtccaca ttatgagttt ctccaatgta atattttgaa gtagtttttg aaaataatat
      361 ataaacaaag tgcgacatta tctttctttg aaaacaaaaa acccgaatat tgctattcgg
      421 gtttaagtgg tacctccagg gatcgaacca gggacacatg gattttcagt ccattgctct
      481 accatctgag ctaaggtacc gttgctaatg cgggtgcaaa tatagtgcga tttttatttt
      541 atccaaaaca attataaaaa aaaatctatt cgtatcttcg cctttttaaa atctatcgga
      601 atgcttttag ctattgatgt tggaaatact aggattaaag gtgctgttta tgagagaaat
      661 acacttatta atcaatttgt attttcaagt gtagaagtta aaaataatat tgaaaatatt
      721 ttagaaaaga atccaaaaat cactgattta attgtttctt cggttggaaa tgaaaatagt
      781 attgtcttta attcgttttc agaaaggatt tctattcatt ttgtttctca ccaggatatt
      841 tttccgtttc aaaataaata tgcaacacca actacattag gaattgatag aatggtactg
      901 gcatcaggag caactttgct tttccctaaa aaaaataaat tggttattga tgcagggact
      961 tgtgttactt atgattttat tgatgatgaa aataggtatt ggggaggagc tatttctccc
     1021 ggacttcgat tgagatacga gtcattgcat caattcactg caaaattgcc gttattaacc
     1081 ctggaagagc ctctaaattt tattggaaat tccacaaatc aatccattca ttccggtgtt
     1141 gtaaatggat taatccatga aatagatggg tttatagagc agtataaaaa caaaaatgaa
     1201 aactttacca taattttaac gggaggagac gctgaatttt tggcaaaaag attaaaaaat
     1261 accatatttg ccaattcaaa ttttctgtta gaaagtttaa accaattttt tcaatacaaa
     1321 atcaacaatg attaaaaaaa ttcttataat tttttgcttg atattttcat tcttttctaa
     1381 tgctcaaaat gcatcatcct caccctattc tttttacgga attggagatt tagtgtttaa
     1441 aggaaatgtg gaaacaagat ctatgggtgg aataaatatg ataaatgatt ccattcatat
     1501 caatatcaat aattctgcaa gtttgtctga tatcatgaat actactttta caattggggg
     1561 taatttaaat tatgtaaatt ataaaacagc aagtcaatca gaaacggcaa atagagcttc
     1621 cttagattat atagcattag cggtccctat ttcaaaacga cttggtgcgg cgtttgccgt
     1681 aatgtctttt acttccgttg gctatcaaat tcaaaataat tatgttgatg acagtacgcc
     1741 aatatctaga ttcacaggaa gtggagggac aaataaaatt tatggtggcg taggttataa
     1801 aatcaataaa aattttcgga taggggcaga tatgaatttt aattttggta atattacaac
     1861 caatgatatt agttttctaa cgggtattca gtatggaacg agagaattaa atagtagtga
     1921 aataaccgga tttaatttca atactagttt gatgtataat aaattaatca ataaaacgac
     1981 aacaatattt ggaagtttga tttattctcc tgaaagcact ttatctttgg caaatgaaag
     2041 aaagattgcg atagttcagt tttcagaatc ttttggggaa ttagtggtta atgaagaaga
     2101 tgttcctgtt aatgatacta aagtaaaata cccttccaaa attggaattg gatttggtgt
     2161 cggggaagtg agaaaatggt ctgttggagc agaagttaca tttatgcaag gcagtaactt
     2221 tggaagtagg tttgacgata taaacgatgt ttcctatgaa aatgttacta aatacagttt
     2281 tggaggttac tatatgccgg attataattc cttctccagt tattataaaa gaataacgta
     2341 tagaggagga tttaattatc aaaatacagg attaattgtc aataatgagt cgattaataa
     2401 ttttggcata acttttggat taggtctgcc aataaaaggt tcgttctcca atgtaaacgt
     2461 tggttttgaa ttcggtaagc gaggaacaac aagttctgga ctaatacaag aaaattattc
     2521 aaacttaagt gtaagttttt cattgaatga caaatggttt attaaaagaa aatatgacta
     2581 aaagagaaat ttttaatatt atgaaaacta aagtaattat tttatttgcc tttatactag
     2641 taacgtttaa tggctttgcc caaagtgctg caaactgtgc agaaaaacta tcaatttttg
     2701 ctgaaatggc aaaaacaaaa aactatggag aagaaaccta caaacatctt caagagttaa
     2761 gaaaagattg cccttccttt agtgaagcta tatatattta tggtgaaaga attatcaatt
     2821 ccagaattga taacgctaaa acacctgcag ataaagaaaa ggaagtacgt gattttatta
     2881 aattacaaga tgaatttaaa actaattttc ccgctaaagc aaaaggaatt gaagttaaaa
     2941 aagcaatggt cctttttgaa aataatagtg gcacttccga tgaagtatac caacttttag
     3001 acacagcttt caaaaatgat aaagataatt tcagtaatcc aaaagcatta tatgtttact
     3061 ttgaaattta tgtaaatgat tttgaagcaa ataaaaacgg aattaaatta caagatattt
     3121 tcaataaata tgatcaagtt tctgataaaa ttgatgaaga agagaaaggg gtttcagatg
     3181 ctttagatgt tttattgcaa aaggaagaaa gtggaacaga attaacagat aaagagaaaa
     3241 gagcaaaaga aaatcatcaa attaacttag atgctttcga aactattcat tccagcatgg
     3301 atgctaagat tgctcaatta gcaacgtgtg agcgtttaat tccattctta ggagcaagtt
     3361 ttgaagagaa taaagcaaat gctgagtggt taaaaagagc agcagaccgt ttagataaaa
     3421 aaggatgttc atctgacccg ttgttttcaa aaatatcaga tgcattacat tctttaaatc
     3481 caacagctga atcagcctat aatttagggg tatctttcta tacaaaaaag aatactgcta
     3541 aagctttaga gtattttaat cagtctgctg aattacaaaa agataacact aaaaaagcaa
     3601 atgtttatta tacaattgcc agaaatattt atggaaacgg taacaaagtt caagccagag
     3661 cttatgctga aaaagcgtta gctgcaaaac catcctttgg agaggctcat gtttatattg
     3721 ctcaattgta tgcgaatagt gcgaatgact gtggaaatga tccatttgaa aaaagagcga
     3781 tttattggtt agcagctcaa acagcaagaa aagcaggaac agcatctgga aatgcagcag
     3841 cttcctctta cgataaattg gctccgtcta aacaagaaat ttttaactca ggaagagcag
     3901 gtcaaagcat ctgtttcaaa tgttgggttg gtaaatgtgt tacagttcca aatttgtaac
     3961 taaatgctta gcataaaaaa aataataaat ttcagtgtca cagtatttgc tgtgacattg
     4021 ttttttgcgt gtgaaagtaa ttttaaagaa attcagaaaa ttaatatttc tgaatttatg
     4081 gcttctggag aagcggattc cgtgaattta aaatataccg attccggtag aatcaaagcc
     4141 attttgatta gtcctaaaat gtttgattac ggtaccgttc aatacccgtt tacagagttt
     4201 ccaaaaggaa ttgatgtaac actatttgat gaaaagggta aaaaaaatta cattaaagcc
     4261 gattatgcca tcacgttcaa agggacagat ataattgact tgcaaggaaa tgtcaagatg
     4321 actgctgacg gagggcaatt tttagaaacg gaacaattgt attacgacca aaaaaatgaa
     4381 tggtttttta cggaaaaaaa gtttaaattt acggacccta ataaaggact ttcaacaggt
     4441 gaaggtctag attttagtaa agattttaaa cgaattaatt tccaaaaagt gtatggtttg
     4501 ataaatgatg ccgagtaaat caaagtcaaa aacctttaac ctaatacctt tcattaaatt
     4561 atgacatacc ttaaatacat acaatacttt tatctttttg cagcaggatt ttttatttac
     4621 caaggattta taaatttaaa ttctgaagaa aaaagtccgt ggatgatgtt cttttttgcg
     4681 gtgatggcaa tttttatgtt ttttttcaga cgaaagttta tgaagaaaat gaataaccaa
     4741 gataaaaagt aattctggat ggaagtcagt attatcatca tctgtttact gctttcagca
     4801 ttcttttccg gaatggaaat tgcctttgta tcctccaata aagtttattt gggtatcgaa
     4861 aaactacaag agggttttgt tgccaaaatt ttgactaaaa ttactgaaaa gccttccatg
     4921 tttattgctt ccatgttagt ggggaacaat atcgcactag ttgtttatgg atttatgatg
     4981 ggtgatctaa ttatcaaatt attgtttccg gattatttag aaattcataa tttgcctttt
     5041 cagatattga tgattcaaac cttgatttca acagctgtaa tccttatcac agcagagttt
     5101 ttgccaaaag tatttttcca aatttacgct aataattgca tgaaaatttt tgcagttccg
     5161 gcctattttt tctatgaatt gttatatttt atttcggcat tcactatctg gatctcagat
     5221 tttgttttga aaaaagtatt taaaacacaa ggagatgaaa cgcaattgtt ttttagtaaa
     5281 gtagaacttg gaaattttat ttcagaacaa atgaattctg ttgaagacca cgaatcggtc
     5341 gattctgaaa ttttaatgtt tcaaaatgca ttagaatttt ccggtgtaaa agccagagat
     5401 ataatgattc ctagaactga aatttcggca gtagaaattc atgattccgt aagtgaaata
     5461 aaacaattat ttatagaaac aggttattct aaaattttag tatttcaatc ttctttagat
     5521 gatatcattg gatatgtgca ttcttttgaa cttttcagaa aaccaaaaac aataaaatca
     5581 gtaatgattt ctgttgagtt tgtgcctgaa acaatgttta tcaaagatgt actcgattta
     5641 cttaccaaaa aaagaaaaag cgttgccatt gtactagacg aatatggcgg aacatccgga
     5701 atgattacag ttgaagatat tgttgaagaa ctttttgggg aaattgaaga tgaacacgac
     5761 aaggatgaaa cactggttga ggagcaaata aacgagaaac aataccgttt ttcaactcgt
     5821 ttggatgtag aatatattaa tgaaacctat aaattaaata ttcctgaaga agattcctat
     5881 tctactttag gaggttttat tataaatcat accaaagaat ttccttctaa aggagaaaaa
     5941 atttcggtcg aaaattttca ttttgtaatt gaagaagctt cgaatagaaa aattgaatta
     6001 gtgactttga caattacgaa ttaaagcatt actgctcata ctattcctat atttaggctc
     6061 acattttctt agtattaaag ccaaactact tttatttaag ttggaataga gtgggacatg
     6121 caaaaattac acaaattatc tcgatttctt tataattatt tcatagataa ttgtattttc
     6181 gcaaactgaa ataaaaataa aacataaata aaatggcgat tttaggaaaa attagacaac
     6241 gttcatttat tctaattgtg attattgctt tggcattatt ctcttttgta ctggctgacg
     6301 taattcaaag cggtggcttt gctcaaactt caacaaatgt tggtagtatt aatggaaagg
     6361 atattgcttt cgaagaattc cgtttgaagg tgggtaacac tgaaaaaggg ggtcaaggaa
     6421 tgtctaccat tcaagcagta aaccgtgttt gggatcaaga agtaaatgtt gctcttttaa
     6481 cagaagaatt agagaaatta ggcattagaa taagtgaaaa acacttaatt gaaaccttga
     6541 aaaatgatcc aaattttggt caaaatccaa gttttcaaaa tgagttaaaa caatttgata
     6601 ttaataagta cagagagtat atcaaagcaa gttctaatcc gcaagaagtg gaatattttg
     6661 aatctactga aaagaatgcc aatattaatt caaaatatca aatttacagc tcattagtga
     6721 aaggtggatt tttcacaact caaaacgatg caaaatttaa atataacttg caaaataata
     6781 aagttacgtt tgatttcgta gcggttcctt tttcttcaat taaagacagt gatgtaaaag
     6841 ttacggatga agaaataatc gaatacatga aaaagaacga gaaaaaatat aaagcagaag
     6901 aaacaagaga gattgaatac gttgtaattg aagacaaacc ttccgatgct gataaaactg
     6961 aaattaaaaa taaagtggct tctttattga attctaaagt ggtttataat aatgaaacat
     7021 ctactaacga tactattgct ggtttttcaa atacaaaaaa tgtaattgaa tttgttaatg
     7081 caaattctga tttcccttat gattcaacgt atgtaggtaa atcagattta cccgctgaac
     7141 atgctgagca aatttttaat ttaccggaag gacaaattta tggtccttat gtatatggtg
     7201 aatactatgc cttaagccgt tctatgggta gaaaagctgg agcaaaagcc agagcaagcc
     7261 acattttaat cagttttacc ggatcaagag ctcaaccaaa agaagacaga acaaaagaac
     7321 aagctaaagt aaaagccgaa gagttattgg gccaaattaa tgcaaaccct gattctttta
     7381 ccatgttggc atttacggca tctgaagacc aaggttctgc tcaaaaaggg ggtgatttag
     7441 atttctttgg tccaggtcaa atggttcctg catttaacga tttcgtgttc aataacccaa
     7501 ttggaaaagt tggtttagtt gaaacagagt ttggttatca tattattaaa gtgacggata
     7561 aacaagacgc aatccgttta gcgacaattg ctcaaaaaat tgaagcttct gaaaaaacag
     7621 ctgaggttgc ctataccaat gccgttaaat ttgaattagc agcggctgaa aaatcatttg
     7681 aagcagcggt taaagaagca aaacttactg ttgttcctcc tgtacgcgta aaagcgttag
     7741 atgaaacatt aggtcaaatg ggagctcaaa gacaagtaat tcgttgggct tttgaaagtg
     7801 atactgaatt gaacgacgta aaaagatttg aaattgctaa agtggggcat ttaattgcaa
     7861 aacttaaaaa agtaaatgaa gaaggtttat tgaatattga agatgctcgc cttcaagtgg
     7921 aaccaatctt gaaaaataaa aagaaagccg aaaaaataat ggcaaaaatg aaaggagcta
     7981 ccttagaagc tatggctaca gcaaatgctg ttcaggttca aaatgctgct gatgtaacat
     8041 tagaaaatcc tgtagtggct actttaggtt atgaaccaaa agttgttggt tttgcctttt
     8101 caactcctgc aaacaagctt tctgctccaa ttgaaggaaa tgcaagtgtt tttgttgtta
     8161 aaacaactaa agtgacaaaa gcattgccaa ctcaagatta ttccgctcaa ctcaatgcgt
     8221 taaaatctca aaatgcatcg ggtgtgaatc gcgtattctc agccttaaaa gataaagcag
     8281 aaattaaaga caatagattc cagtttaatt attaattcaa actgtatgtt tataagaaat
     8341 cccaaccgat aagttgggat tttttgtatt atagcttgac cttcaaattt gagagagcag
     8401 aagtttctat tttaaaatca tatcatcata acaaataact gtatcaaaac ctctttcttt
     8461 aaaatagccg gaaggattct cctttgaaat ggctaataca aaatcgccgc cccaagcacc
     8521 taaacttttg attacacctt taaagtcatc aaaaaaagct tctttaatgg tattcatctc
     8581 taagacttca ctaaggatgc tttcatgttt ttctatttca taagcaaagg tctttaaatc
     8641 agaagttgat aaaatggact tagtaatggc gtttattttt tcaatcgttt ttccaatttc
     8701 attttgctta ttgatgtaat ttgcaatggc cgatttacta ctttgctttc tattcaaata
     8761 cacaaaatat aagtgatttc taaattcagg ataaaaagct acttttttat aacttggttg
     8821 attatttatt aattgataga taattggatt atctttttta gcacaggcaa tatcataacc
     8881 gcttcctcca aaacttcgtt gtaacaattc aaaagcatca agctctaacc attgtccaat
     8941 gttattaatt agtgtggaag aagtgcctaa tccccagttt tttgggaagg ataaattagt
     9001 ttcaatagaa tagccattat tacctaacag aaaatccggt ttcatctgaa aagcctcgtg
     9061 taaaatttca atcaatgttt tatgaatagg attttcgctc attgttttgt tttcttgaac
     9121 gatagaatca aaagtaaaca cctcttcaaa ccaagtgctt ccatctgcat caatgctttt
     9181 ccagttaatt tcgtttgttt tgccgggttt taccaataag ttttgaccaa attttgtcgg
     9241 caatgccaag gcaagtgctc catctaaaac gacatattct ccggttagaa gtaattttcc
     9301 gttgctgtaa aaagtttttt tcaagggtat tttattttcg caagtgttcc ataaattctg
     9361 aaacagaagc atgtgaaata gtattgtctt taaaataggc taaaacctga atgcgttctt
     9421 cagccgttgc atggtattga tttaaaatat tatttaagtg cattttcata tgtccttcct
     9481 gaattccttt ggttgtgagt gaacgcaaag cagcaaagtt ttgtgccaaa ccaacagcgg
     9541 caactacttg catgagttcc tttgcagaag gtttttcaag tatttctaaa tttaatttaa
     9601 ctaaaggatg taaagaggtt aatccaccaa ctgttcctaa agccaaagga atctctagcc
     9661 aaaaactaaa aatacccttt tcaatttttg catacgataa actgctatat ttcccggttc
     9721 tactcgcata agcatgggcc ccggcttcta cggctctaaa atcattacct gtcgctaaaa
     9781 caacagcatc tacaccattc ataatgcctt tgttgtgggt aacggcacga taaggttcga
     9841 ttgttgctat ttgcacggca cgaacaaatt tttcggcaaa ttccgttggg ttttcgatgt
     9901 gtttttcagc taattcttca ataggacagg aaacttctgc tcgaacaata caattggtca
     9961 cataattgga aagaatactc atgactactt caatgttttt ttcttcttca gaaaaagcag
    10021 tgtaattttg agcctctgct ttcaatgttt tagcaaattg ttccaaacaa gagttgataa
    10081 aattagcccc catactgtct tttgtttcaa aagtggcatg cagttggaaa taattttcta
    10141 aatcggaggt tttgtctttt aagacaatat ctaaaattcc gccacctctt ttttgcatgt
    10201 ttttagtgat gctttcggtt tcagaataaa attttgcttt cgtttgatta aaaaaagcaa
    10261 ccaattttga agtttcacct ttgtatataa aatgcacttg accaattttc tcagtattca
    10321 aaacagtggt tttaaatccg cctcgttttg accaaaattt agctgatttt gcggcagccg
    10381 caaccaccga actttcttct ataaccatcg gaactgtata attccttccg ttgatgttga
    10441 aattgggagc aattcctaac ggtaaataaa aattggaaat ggtgttttca atgaattcat
    10501 catgcaattg ttgcagtttt tcatcactat tccaatagtt ttgaagtaat gcaacagcaa
    10561 ctttgggttc agaaaaatag ttttgagcaa tccaatcgat tttttcaatc ttggataatt
    10621 ttgaaaatcc ggaaacttcg tggttcatgg ttgttgctta aatcttatgt aatgcaaaga
    10681 tagtagttaa gaatttataa aatcagagtg aattcttgta tttaataaac ccaaaaacaa
    10741 ttatttcttt tttttgtaaa ttgactttaa aggtgatagt atatcctttg aaaatgtaat
    10801 ctcgaattaa ctcatcatca aaataaattg atttcctgaa attaaaagga gtttttaaat
    10861 cttttgaaat gtatcttaat aaatcgtttt tgaattttct tgctgctttt ggtttgtctt
    10921 ttgcaatgaa tacaacttgt tcagatagat cattaatgaa tttttcagaa aatctaatct
    10981 tcataggttg aaattatttt ttccataacc aaatctaatt cttccataga aacacattta
    11041 gattttccgc tctcaatgtt tttaagttcc tcttcaagaa ttcttttgtt tttttcaaaa
    11101 gaaggatcct cttgaatgat ttgcacttca ttctctgaaa atgaactcaa taattcaaga
    11161 attttagatt ttaaattggg ttgaaattga attcgaatag tttccatcag gattagatta
    11221 atacacaaat ttaataaaaa ttaaaattag aatgtaattt ttttaaaatc tcaaactttt
    11281 taaaaaatct aaaattgcta tttaacacat aaaaggacga attattccat ttttttcact
    11341 aaaattgaac gtttttttaa catgccaact atgaatcatt ttaataaagt aattttcttg
    11401 tttctattta tttcttttgc tgcaattggg caacaaaaaa taacattaga agatatttgg
    11461 gcttccggtg ctttccgaac taaagggatg gatgctcttc aggctatgaa aaaaacgaat
    11521 caatatacgg ttcttaattt tgacagagct tcaaaaacct ttcaaattga tttatatgat
    11581 tttgcaacat taaaaaaagt aagcacatta gttgattcta aaaatcacgt tgagttatca
    11641 tctattgata gttacacctt taatgaagca gaaacgcaat tgttgattgc tttaaattct
    11701 gagcaaattt atcgtcattc ttccgttgcc gatttttata tttatgatat tgctaccaaa
    11761 actcttcaaa aagtagccga ttataaaatt caggaaccac tattttctcc ggatggaaaa
    11821 aaaattgcct atggttatca aaatgattta tttgttttag atgtggcgtc taaaaagcat
    11881 acgcaaatta ctaatgacgg aaagaaaaat gaaatcatca acggaattac tgattgggtg
    11941 tatgaagaag aatttgcttt tgtaagagcc tttgattgga acaaatccgg gacacatttg
    12001 tcctatataa aatttgatga atcaaatgtg cctgaattct caatggatgt ttttggaaaa
    12061 gaattatacc catcacaaga agtgtttaaa tacccaaaag caggagaaaa aaatgcgttg
    12121 atttctttgc atatttatga tgttgcttcc aatgcaacta ataaaattaa tttaagtcaa
    12181 tacagcgatt tttatatagc ccgaatgaaa tggaccaacg acgcgaatgc gttaagtgta
    12241 caagttttga accgtcatca aaataattta gatttacttt ttgtagaggc aaaaacgggt
    12301 acaactaaag ttgtattaaa tgaaaaggac acagcctatg tagaggtaac tgacaaccta
    12361 acttttttgc aagacaatag ttttatttgg acctcagaaa aagatggttt taatcacatt
    12421 tatcattacg ataaatccgg aaagcttcgc aatcaaatca ccaaggggaa ttgggaagtg
    12481 acttcctatt atggttttga tgagaaaaat ggacaagttt attatcaatc ggttgaaaat
    12541 ggttccatca atcgtgatgt ttataaaatt aaattggatg gaaaaagtaa tgttcgatta
    12601 accaacaaaa ccggaaccaa caaggcaact ttcagtccta attttcaata ttttatcaat
    12661 tctttttcta gttcaaccga accaacttcg ttcacattac acaattcaaa agacggaaag
    12721 caagttcaat caatcgttga taacaatgaa ttagcttcaa aacttaaaag ctacaatctt
    12781 cccacaaaag aattttttga attaacaacc gaaaaagggc ataaattgaa tgcttatatc
    12841 atcaaaccaa aggattttga tgcgtcaaaa aaatatccgt tattgttgtt tcaatatagc
    12901 ggtccgggtt ctcaacaagt ggcaaatcaa tggttaggag caaatgatta ttggcacatg
    12961 atgttggctc aacaaggcta tttagtagct tgtgtggatg gccgaggaac tggattcaaa
    13021 ggtgcagctt tcaaaaaagt gacccaaaaa gaattaggta aatacgaagt ggaagatcaa
    13081 attgatgcag caaaagtatt gggaaattat acttatgttg ataaaaccag aatcggaatt
    13141 tggggttgga gttacggtgg atttatgtcg agtaattgta tcctaaaagg gaacgatgtc
    13201 ttcaaaatgg cgattgcagt tgctccggta accaactggc gtttttacga ttcaatctat
    13261 acggaacgat acatgcaaac gcctcaagaa aacgcaagtg gttatgacga caattcacct
    13321 atcaatcacg tagataaatt gaaaggaaat tatttaatca ttcacggaac agcggatgat
    13381 aatgttcatt ttcaaaatgc aacccgcatg attcgagcct tgcaattagc caataaacaa
    13441 tttgatcaag ccatttatcc ggacaccaat cacggtattt atggcggaaa aatacgcttg
    13501 caattgtata ctaaaatgac aaactatatt aaccaaaacc tataactaac caaaacaaat
    13561 tttatgagtg aagtagcagt aaaatcagga catccaaaag gactttgggt attattcgga
    13621 accgagatgt gggagaggtt caatttctat ggtatgagag ccattttgac cttgttcatg
    13681 gtaaacgctt taatgaaaaa tgaagctgaa gcttcgttga tatatggtgg tttcctaggg
    13741 ctttgttatt tgaccccaat gttaggcggt tttattgctg accgattttt tggaaacaga
    13801 aattgtattc ttttaggtgg tttaatgatg gctgtaggac agtttttgtt gtttctaagt
    13861 gctagtgttt ttggtacaaa cctttcttta gcagatacat tattatggtc ggcactagga
    13921 ataatcattt ttgggaatgg tttttttaaa ccaaatattt cctcaatggt gggtagttta
    13981 tatccaaaac aggaaaaaag taaattagat acagctttta ctattttcta tatgggtatc
    14041 aatatgggtg cctttttagg tcaattaatt tgtccaattg ttggagatgt taaagatgta
    14101 gatgggatta gagatattca tgcttttaaa tatggatttt tagctgcggc aattgcaatg
    14161 ttaattggaa ctgcaatatt ttatttttta aagaataaat atgtggttac tccggaagga
    14221 agaccaattg gtggtttgcc aaaacacaac gttaatgatg attatgaaga aggagagtct
    14281 caaaaagcaa ttttctctca aacagcttta attggagcag ttgttggttt cattggattg
    14341 ggattagttt ttcattacgt gtttgatcaa aacattattt attcgattat ttatgctagt
    14401 ggtatttcat tggcgggttt aattatttcc gattcttctc ttacaaaagt tgagagagac
    14461 agaatcttag taatttatat tgtagccttt tttgtgatat ttttctgggc cgcttttgag
    14521 caagcgggtt cttcactaac attcattgca gataatcaaa cggatagaaa tttctttgga
    14581 tataatatgc cgccttctat ggtgcaaatt ttcaatggtt tatttgtagt gatttttgcc
    14641 gttccattta gtttactttg ggataaatta agagaacacg gaaaagaacc aatttcacca
    14701 gtaaaacaag ccgttggatt agctctaatt gctttaagtt atttcatcat agcttataat
    14761 gtcaaggatt taggaaatga tggtttgtta gctattaagt ggttaatatt attatactta
    14821 attcaaacgt gtgccgaatt atgtttgtcc ccaataggat tatcattagt aggtaaatta
    14881 tctcctaaac gttttgcttc tttgctatac ggtgtattct tcttatcaaa tgcagccggt
    14941 tatgcgttag cgggtacttt aggttcgatt ttaccggcaa ccggtgaaaa atttaacaaa
    15001 gcgaaagata tgggtatcga tttacaagca gttttagata aaacagttac accaacggcc
    15061 gaacaattaa aattattggc cgacaatcaa attagtccaa caaatcctgt ttttgctggt
    15121 tttgaaatca ataatttgtt cgaattcttc atggttttcg taattctatg tggattagca
    15181 gctgttattt taatggctct tactcctaaa ttgaaaaaaa tgatgcacgg tgttcgttaa
    15241 ttagttttta aaaatatttt catatctttc aaacctgcga aacattctcg caggtttgtt
    15301 atttaatcta ataatttatg tggaaaagtc atcctaaagc actgccgtat ttgtttttgt
    15361 ctgaaatgtg ggaacgtttc gggtattatt taatgattgg tatttttact ttatacctta
    15421 aagatgtaga ggccggtttt gctatgaccg aaaaagaagc atccgattta tacggaacat
    15481 ttatcgcttt ggtttttctg acccctttta ttggtggttt aattgctgat aggtattggg
    15541 gttacaaaaa gtcaattatc attggtggat tgatgatggg tgccggttat tttatgatgg
    15601 gaattcataa tataacggta ctttacattg ctatgacgtt agttatcgta ggaaatggtt
    15661 tttttaaacc taatatttca actctgctgg gtaattttta taatgaagat aaattcaaag
    15721 aaaaaaaaga tgagggttat aacatattct atatgggaat aaatattggg gcctttattt
    15781 gtaatttctt tggtgcagct ttaaaaatcc tttttggttg gcaatatgct tttatggcgg
    15841 ctggggtagg catgtttatt ggagttattg tgtttatttt aggaacgaaa cattatggaa
    15901 acaaaacaga aaagaaaggt gttcaagaag gcgatatgcc cttttataaa attgtattat
    15961 tcattttatt accgtcggca cttttcggaa taataggttg gtttatcaat ggtgttcaat
    16021 cggatgcaaa tccaaattgt tcaatttttg gttctgatag tacagatgct tttatttttg
    16081 cttgtatacc ggttattttc ttttatgcca gtttatattt caaagctaaa aaagaggata
    16141 aaagaccaat tggagcgttg ttaaccatat ttggcgtagt tattttgttt tgggcggttt
    16201 ttaaattaaa tggttcagca ttaaataatt ggggtgacaa atatactgac agagagctaa
    16261 caggtgtttc ccagactatt gcttcaaaat tagttttaac agacacattg gtatacaaaa
    16321 gagattcagt agctctttat gatgaagctt tcagaattca aaagaaagat gggaaagtta
    16381 taaaagtagt tgattatccg ttgtatttta gaaacgttaa aaaagaaaaa ttacccgaag
    16441 agaatagtaa aattttcaca tggtcagcta atttaagcca atccattaat ccgggttggg
    16501 taatcctttt aacgccactt gtggttgctt ttttcacctt tttaagaggc aggaagaaag
    16561 agccctcaac acctacaaaa attgcattcg gattattgat ttcagcttta tctgttttag
    16621 tcatggtggc agccgtcaat atgggaggga atggttcgga aaaagtgagt gtttggtggt
    16681 tagtggcgaa ttacggagtc ataaccatcg gtgaattgtt tctgagtcct atgggattgt
    16741 cagttgtttc aaaattaagt ccaaccaaca ttacttcact catgatggga gggtggttcc
    16801 tgtcaacttc tattggaaat aaattaagtg gcgttttagc tagtttatgg gatacctatg
    16861 ataataaagc tgacttcttc tggataaatt tcggattatt gatgtttgca acgttactca
    16921 tgtttgttct tttgaaacaa ctgaataaag ttatgaaaga aaaaggaatt aattaaaaat
    16981 aacgaaatgt atacagtaga agaaattcat gattttaaag gaaagtaccc aaaacaattg
    17041 tggtaccttt tctttagcga aatgtgggag cgtttcagtt tttatggaat gcgtggcatg
    17101 ttggccgtat ttatggtgag ccagctgatg atggatgaaa aaacagccaa tttacaatat
    17161 ggagctactc aagcatttgt ttatgctttt acatttattg gaggattatt tgcagataaa
    17221 attcttggtt atagaaaatc acttttttgg ggtggtttgt taatgattgt gggaagcatt
    17281 attttggcaa tagaccctaa acaatttttc tttttcggaa ttagttttac aatcattggg
    17341 acagggtttt ttaaaccaaa tatttctacc atggtgggta aactctaccg cgatggcgac
    17401 aatagaagag acgccggttt ttcttttttc tacatgggag taaatctggg agctttattg
    17461 ggtggttata tttgtattgc cgtggcaaac ggaacattat gggaatcatt cattcctgag
    17521 catttgcgat ggaatattgc tttcggattt gcggcagtgg tgatgattgt gagcttactt
    17581 acttttacac aaacacaaaa aacattagga gaaataggtc tttctcctct ggctcattta
    17641 gaaaaatcta aaaggagaac tttagaaatt gcgacttata ttggctcatt agtaatcatt
    17701 ccaattatta tgaccatggt tgctaaaacg gaatataccg attattttat gtttataatc
    17761 ggaccattag ccttactgta tttgtttttt gaaatgagaa acttttcatt agtggaaaat
    17821 aaaaaattgt tagcagctct agtctttata ctattttcaa tcttcttctg ggcctttttt
    17881 gaacaaagtg gcggttcttt aagtttattt gcagccaata atctagataa taccgttctg
    17941 ggtgtaaagt tagaccctaa tggagtaaat aattcttcga attccttttt tgttatagcc
    18001 tttgcagcat tagtaggaat ggcttggctt tggatgaata agcgaaaaat tgagccaaat
    18061 acggtagtca aatttggttt agcttttctt ttcttagcgg gaggattcta tgtgttttat
    18121 tatacaaaat tcttttcaaa cccggatggg attacttctt tagatttatt tacaatgggt
    18181 tggttggtga ttacttttgg ggaattgtgt ttatcaccca tcggaatgtc ggccatgaca
    18241 aaactttctc ctcaaaagac acaagcggtt attatgggaa tgtggttttt agcgagtgct
    18301 tacggacagt attttgccgg tattcttgga gcgaatattg ctgaagcttc tgagaattct
    18361 tcaaactttg acaaattaat agtatatgcg gatggttata aagatttagc tatctatgct
    18421 ttagtttttg gattagtttt aattgccatt tcaccttttg tgaaaaaatt aatgcaggat
    18481 gtgaaataat tattttaaat ttcggcatta tttttgaaat tcaacttata aaaaaatgtt
    18541 atgaaaaaat tactttttgc tctttttcta attattacaa cagtttcatc acaagctcag
    18601 gaaattaaat ggatgacgct tgatgaagca ttagcgttac aaaagaaaaa cccgaaacca
    18661 atatttatgg atgtttatac ggattggtgt ggaccttgta aaatgttgga caaaaatacc
    18721 tttcaaacca aagaagtgtc agaatttata aacgccacat attatgcggt taaatttaac
    18781 ggagaaggaa attcggtagt taattataaa gggaaaaaat atacaaatcc aggctacgat
    18841 gctacccgta aagggagaaa cagtatgcat gaatttacag gttttctcaa tgttgaaggt
    18901 tatccatcaa tgtatattct agacaaaaat ggagatatag cgaaagtggt tgttgggtat
    18961 tatcaaccgg aacaattatt agcggaatta aaaactgtga aataaatgaa taagattatt
    19021 ttatattttg tctttttttg gtctcttcaa acgttgagtg cccaagaatt aaagtggcaa
    19081 acagatatta ataaagcgat agaactttct acaaaaagta aaaaaccact tatgttattt
    19141 tttactggga gtgattggtg tgggtggtgt attaaattgc aaaaagaggt ttttcaaaaa
    19201 cctgaatttg tagaatggtc taaaaaagtg attttagttg aattggattt tcctaaaaaa
    19261 acccaattag aagcaagcct aaaacaacaa aatgatcaat tgcaacaaat attccaggtg
    19321 caaggttttc caacaatttg gtttgtcagt ccgcagaaaa acggaactca aattaatttg
    19381 caacaattag gaaaaaccgg ttatgtggct ggaggacccg aaaaatggtt agaggcggta
    19441 aatcaattta tgcctaaata agttagttta attcaatctt ataatatccc ttttcactgg
    19501 ttgagtgaaa agggatgttt ttttgtaaac aggtttgctg ccaattctca actaaatatt
    19561 tataattact gccatcggca ataataattg ctggatttaa ttctccaatt aaccgttcta
    19621 aatttatttt tggggattgg gttaataaaa ctatgtttgg ctttatgggg atgctataaa
    19681 tgcctccttt atcaatgact aaaattttag tatcatttat ttgtaaaatg tttttgagtt
    19741 ttgtgacaga gccgatttgg ccaaagttgg cttgaagata attttttagg acataattgt
    19801 ctgcaatatc tttttcatta ctaaatattg taagcgagtt atttgttttt tttgccaata
    19861 tggtttgttt tgggtattga aaaataataa actcatcgtt tgacttatga tattcaagcg
    19921 aaccaataat tgataattga aatagaagaa tgaaaattaa agcaatcagt attctattga
    19981 attttggttt tttaaaccat aaaacgattc caattaatgc taaaagagag ctgaatagaa
    20041 gtgttaaatt gaaaggaatt cccttgatta tgaatttttc taaagaggaa ataaaagcca
    20101 cataacgatt catcaatccc actaaaaaag ataacagatg aatgaaaatt tcagttggaa
    20161 ttctcataac agcaaatagc aaagctatca ttccacatac taatatgagt gttaataagg
    20221 gtattaccac caagtttgtt agaaagaata atccgggaaa ttgatgaaaa taatataaac
    20281 tcaaaggaag tacaccaatt tgtgcagcaa aagaaaccgt tagaatgtca taaaaatact
    20341 gaataatttt attgctggat ttccatagct tagcgaataa aggctgaatg gtcacaattg
    20401 aaaacacagc acaataactt aattgaaaac ctacgtcaaa taagaaattg ggacgaataa
    20461 gtaatataag caaaatggag gaacccagtg tgttgtaaat attggtttct cgtctcatat
    20521 acattccaat tgtgataaag gaaaacatgg taactgcccg aacaacagat gctgataatc
    20581 cggaaagtaa agcaaatgac caaagaatga aaataagcag aaacgggaga ataattttgc
    20641 catacctgaa tcgttccaag ggtttgagta acgttttgaa aaagaataat aatattccga
    20701 tatgtaaacc tgaaatagcc aatatatgaa ttgctccggc ttgagagtat tgattgttcg
    20761 tttcctgatc aatatcttga cgttgaccta gaaataatgc tttaattatt tcgagccggt
    20821 tagaagaaat attggcattt tcaagtttag ttacaattac atttctaatt tgttccaact
    20881 tataatataa attcttctca caactaacaa cactgtaatt attatcctct aaatacaatt
    20941 gatgaaaaac atcctttttt tctaagtggg ctgcatagtc aaattggtag ggattatggg
    21001 gtctgataat tggttttaca aaagcataag ctaaaataac atcgcctatt tgaaattcct
    21061 ttttggcgtc tttttttgac aaaagaatta ataatttacc tgttttatca atcttatcaa
    21121 ccgtttctac ttctaaaaaa tacttatgag taaatttatt gggtcttatt ttttcagaaa
    21181 taacacctcc aacgagtgat ttttcattct taagttcatt ggaataatga agtggttgat
    21241 tatgtttgaa atgcaatgaa taaaccataa aaccaacgaa aaaactcaat aaataagccg
    21301 ataatccaaa ataaaaacga aaaatcgctt ttttattgga aagccaaaaa aacaataaaa
    21361 aaggaatgaa caatagggaa actagaaggc atgcagaatt aatgtcgggt ttaacttcgt
    21421 ttgaagccaa aattcccaac acaacacaaa gcgtgatgtt taaggttgga aatttcatta
    21481 ccctcattaa tacgaaagta ataaaaattt aattaatcag aaaaaaatta taattaaaaa
    21541 tattcgtcac ccaaaatccg gttggcttga gcaaaagttc tttgataata aggttcttta
    21601 gtagaggata taataacgcc gcgttgaaca gaggaatgaa taaaaatgat ctcgtcgtct
    21661 ttaacttctg taactaatcc gacatggtta atcacactcc ggccattagt tctgaaaaac
    21721 accaaatcac ctttttttat ttcattgcga tttagttttt cgcccacttt tgacatatcg
    21781 tttgaacttc tgggaagtga aacatcatag gttttaaaaa cagttgtaac caatcccgaa
    21841 caatccatac cggcagtggt agtcccgcca cctctgtaac gaactccgtt atattgcata
    21901 gccgtattga ttaattgttt tataaaataa gaggtgttgt cattatctgc aacaaaatca
    21961 gtatcctcaa catcggtgat tttatgcttt ttttccgatt ttgtattttc tgtacctttt
    22021 ttgacagaag aactaccagc agatttgttt gttgaaactg ttgccttgtt tggttgaggt
    22081 gctttgtaaa ttcctttttt ttgtgcttcc tccttagagg taacaataac agaagtagtg
    22141 ggtttacaac tcaccaaaaa cagcaacaaa aaggttatta aaacagtatt cttcaagaaa
    22201 ttttaatttt tacaaagtta aaaaaaacaa tttaaaatct ggcaattgaa gcaacaataa
    22261 gttgggcaac tttttcactt gctccttctc ctcctaattt ttcttctaaa atttcatact
    22321 ttttaaggag cttttgacgg tattctgaat ttagaatttt agtcaattcc gctttgattc
    22381 ttttcgaatt acattcactt tgaattaatt cggtaaccac ttcttcatcc atgatcaaat
    22441 tgacaagtga aatgtatttc aaggttatta tccgttttgc aatttgataa gaagcccaat
    22501 tccctttgta acaaactact tctgggactt taaacaaagc ggtttccaaa gttgctgttc
    22561 ctgaagtgac caaagcggca tgtgccacac tcaataaatc atatgtttta ttcgtaataa
    22621 aatgaacggc tttattagtc aaaaattgtt tataaaagtc aaattcctgt ccgggtgcac
    22681 cggctattac aaattgataa tccggaaaat caggaacaat cgccaacatt tcatgtaata
    22741 acttgctgat ttcctgtttt ctgcttcccg gtagtagagc gatgattggc ctttcgtcta
    22801 aattgttttc ttttctaaaa gtttcgtttt caatttttac acggttatga atggcatcaa
    22861 ttagtggatg gccaacaaat tccaccggaa aatgatgttt cttttcataa aaatcttttt
    22921 caaagggtaa aataacatag agtttatcaa aatctctttt aactgctttg attctatttt
    22981 ctttccaagc ccaaatttga ggagagatat aatagtgtgt tggaatacct ttctttttag
    23041 cccattcggc aattcgcata ttgaaacccg gataatcaat aaaaatgagt acatcaggtt
    23101 gaaattgttc aatatctttt ttacaaaaac taatattgtt cacgatggtt tttaaattaa
    23161 agagaacctc cgcaaatccc ataaaagcta aatcccggta atgtttcact aaaataccgc
    23221 ctgttttttg cattaaatcg cctccccaaa accgaataat ggcttttgga tcttttttgt
    23281 ataatgcttt catcagattt gaaccgtgca aatcccctga agcctctcct gcaataatat
    23341 agtatttcat aaattacaca aataaagtag ctatcgttaa ggcaattgtt gctaaaacaa
    23401 ctcctcgtgc catgacatct ttttgaaatt ttagtaataa aaaaaataaa gcaacattga
    23461 taacagcacc taaagcaatt agtttgccta aagagttttg ggattttaaa atagttattc
    23521 cttccagaaa atcatacgaa gtaaaaaaag taataaataa gaatacccca agtattgctc
    23581 cgattatccc caataaaaaa ccgataaata aatctctttt ttgcattata attcaaattt
    23641 ataagtgttt agtttttgaa tggcatgatg agccgtcaaa tcaaattgaa ccggaacaat
    23701 tgaaatataa ccttgttcaa gtgcccattc atctgtatct tctcctttat cttgattaac
    23761 aaattcgccg gttaaccaat aataatcttt gccttgcggt gtttttcttt tgtcaaattt
    23821 ttccatccat aacgcttttg cctgcctaca aactttcatt cccttgattt cttcttcttt
    23881 taatttcgga aaattcacat ttaaaatcac accatccggt aatccatttg ctaaaacttc
    23941 agaggtgatt gttcgtatag cttttttaat tggttcaaaa tctgcattcc aatcataatc
    24001 taatagcgaa aaaccaatgg ccggtattcc ttcaattccg gcttcaacag cagcactcat
    24061 cgttcctgaa taaataacat tgatagagga attagagcca tgatttattc cggaaacgca
    24121 caaatcaggt ttttgtttta aaatttcgtt taccgcaagt ttcacacaat ctaccggagt
    24181 tccggaactg ctgtattctc cttttaattc actatcgaaa tgaactttat tgacaaagat
    24241 agtattgtta atagtaatag catgacccat agcactttga ggactgtcag gtgcgaccac
    24301 aacaacatcg cctaaggttt tcatcacgtc tattaaggcc ctgatgcccg gagccgagat
    24361 accatcatca tttgttacta aaataagcgg ttttttcatt gtacgggtaa ctttaaagag
    24421 aagtcatatt ggttaaaaaa gtaaataatg cgaataaaat tacaaaacta caggtaacaa
    24481 agcataacaa aaacaaaatt aactcttttt aagatttgtc agcatgacaa ttttcataac
    24541 tttgttcggt aatgttatta ttttcacatt aatttacaca aaatataggt ctaaatcaga
    24601 gttggcataa tttttaatgc tgtatactta gaaataaaat gaatacaatt ttgctattta
    24661 tgaaaagaaa cttcaaattg gtattgttgg caatagtttt gtcagcttca ctacttgctt
    24721 ttaaagccac agaaaataat atcagtagca gtgctgatcc ggaaaaagac aaacttttat
    24781 tggaattatt gacttttgta atcgaaagag gacactatag tccggttgat atagatgata
    24841 atttttcaaa aggagtttac aaagattatg tggaagcatt agatccttcg aaacggtttt
    24901 ttattcaatc ggatattgat gaattcgctg aatacgaaac aaaaattgat gaccaaatca
    24961 aaaataaaga cttaactttc tttgagttga cctattctgt ttacttaaaa agaatgaatg
    25021 aaagtcaaaa atattacaaa gaaattttag aagaaccatt tgattatacg gttgatgaga
    25081 ctatcaatac agaatatgaa aaattgccat atgctaaaaa tagttcggaa ttaaaggaac
    25141 gttggagaaa acaaattaaa ctttcaacat tatcttcttt aacggataaa atgaaattgg
    25201 aagaagataa actaaaagga actgacgcca caaataactc agaaaaaaag gaaaataaag
    25261 ctaaagtaga tgctaaacct aaaagttttg aagaacttga aaaggaaacc agagaaagtt
    25321 cattaaaatc gttggatgac tattatacat ttataaaaga attagaccgc aatgattggt
    25381 ttggtgtttt tgtaaatgcc attgcctctc gatttgaccc acataccaat tatatggctc
    25441 cggatgataa agaacgtttt gacgtaagca tcagtggtaa atttgaagga attggtgctc
    25501 gtcttcaaaa gaaaaatgat ttaacggaga tcacggaatt aatttccggt ggaccggctt
    25561 ggagaggaaa agaattggag ccgggtgaca tagtattaaa agtggctcaa ggtgaccaag
    25621 aaccggtaga catcgtcggc atgcgattag ataatgttgt taaaaaaatt aaaggtccta
    25681 aaggaacgga agttcgttta actgttaaaa aagtggatgg ttcgataaaa gtaatttccc
    25741 tcatcagaga tatggttgaa acagaagaaa cctatgcaaa atcaagtgtg gtggaaagaa
    25801 atggcatgaa atacggaatt atttatttgc ctaaattcta tattgatttt gaaaataatg
    25861 acggacgaga tgctgggaaa gatgttgcca tcgaagtaga acgtttgaaa gcccaaggag
    25921 taaagggtat cattatggat gtgcgtgata atggtggggg ttccttgaaa acagttgtcg
    25981 acatcaccgg attgtttatt gagcaaggac cggtagttca aattaaatcc gccggaacta
    26041 aaaaagaagt attgtttgat cgtgatgcaa aaattcaatg ggatggacct ttagtggtaa
    26101 tggtgaataa tttttcagct tctgcttctg aaattttagc ggcagcaata caggattata
    26161 aaagaggtgt agtgattggt agtaaacaaa cctacggaaa gggaactgtt caaaatgtga
    26221 ttgatttaaa tcagtttgtt cgtggaaata cgttagggga tttaggagct ttgaaaacaa
    26281 caactcaaaa attctatcga atcaatggag ggtcaacaca attagaggga gtttccagtg
    26341 atgttgttat gccggacaga tattcttata tcgacatagg cgaaagagac ttggataatg
    26401 ccatgccttg gtctaaaatt gatgaagcac aatattcggt ttggaataaa cagacaaatt
    26461 ttgagaaagc aattgaaaac agtaaaaaca gaatggccgg tgatgagcat tttcaattaa
    26521 ttgataaaag tgctaaatgg accaaagtgc gaagagatgc ggtagtgtat agtttaaaat
    26581 tagatgcttt tttggctgaa caaaaagcag tagaagaaca aggaaaagta tttaaaccaa
    26641 ttacggatta taaaaatgac ttgttgttca aagctattcc ggaagatgaa gtcgtaatga
    26701 aaaaagatga atcatttaaa gaaaaaagag aacgttggtt tgaaagttta tcgaaagata
    26761 tttatgtaga agaagccttg aatgttctgg atgatttaca aaccaaaccg gttgtaaaag
    26821 gaaaagtaga attaaagaaa aaggaaaaag tagtaaaatc ttaaccggca aattgtatct
    26881 ttgagcaaaa gccgaataaa atttgctaat gtccaataca aatcaatcgt taacagctat
    26941 agcacttcag aaattcaaaa agaatttttg gggtgttttt agtttgagtt atattgtgtt
    27001 aatgattttt ttagccctgt ttgcctacgt aattgctccc gataattcgg aaaatgccaa
    27061 tcaaatgcat ttatctattc attcaaaatc acctggtttt gcggtgcaaa tgctttctat
    27121 tccttcttca aaaatagagg ataaaacaat caaagggtat tttttcgggc accccaacca
    27181 agtgacggaa atcccgattt catcctatca aataaaagac aataaactgc aatatgttga
    27241 atacacagag gataaaacct tgcgattagt aaaagaaatt acgttagata aatttataga
    27301 ctacaactcc ttcaagcagg ttgaacaaga acttattacc aacaaaaagt tctacttagg
    27361 aacggataaa tatggtcgcg acttattgag cagaatgtta gtaggctcgc gggtttccat
    27421 ttccataggc tttgtggcag ttttaatttc agtggttgtt ggactgtttt tcggtgcttt
    27481 gggtggctat tatggtggaa aaatcgatgc gttagtgatg tggttggtca atataatttg
    27541 gtccattcca acattattat tagtgattgc aataacatta gcactgggaa aagggttttg
    27601 gcaagttttc gtggctgtag gtctaaccat gtgggttgaa gttgccagag ttgtccgtgg
    27661 acagattatc agcgtaaaag aaatgcaata tgttacggct gctcgtgcct tgggattttc
    27721 aaacggaaga attatcctga atcacatttt accaaatatt atggctcctg tgatcgttat
    27781 ctctgcagct aattttgctt ccgccatttt agtggaaagc ggattgagtt ttttaggact
    27841 cggtgcacaa cctccaattc caagttgggg aagtatgatt aaagaccatt acagttatat
    27901 tattctggga aaaccttatt tggctctggt accgggaata gcaattatgt tgattacatt
    27961 agcatttatg atgacaggaa atgccctgcg tgatgcctta gatgtcaagt cttaattaaa
    28021 attcagtatt aatgaaaagt tgtttcttaa tgattgccaa aagacaaaat tggtttcgcc
    28081 tttttttcat gatagtggga attgttcttt ttggatgcaa aatggataat caggaaagaa
    28141 cggaattacc ccaagaaata aaccggcaaa cagttacaat ggattttgat ttttatccta
    28201 cttccacaac taaccaaatt ataaagcaca atttttatag gttatcttat tcagaaaaga
    28261 atgaacaggc agaatgggtt gcctatgaat taaaaggaaa tacctttcaa aataatttca
    28321 atcgacctta ttttgtggca gacccattag taaaaaccgg ttctgccgac tggaaaaatt
    28381 acaagaattc aggttatgat aaaggtcatt tgtgtccggc aggcgacatg aagttttctg
    28441 aaaatgcttt tgaagacacc ttttatacct ctaatatttc tcctcaaaaa ccggatttta
    28501 atgccggagt ttggaatcgc ttagagcaaa aagtgaggta ttgggcagac aaatatgatg
    28561 caatttatgt agtcacagga ggtgtattag aaccaaattt aaaaaccatc ggaagagaaa
    28621 aagtggcagt tcctgatttc ttttataaag tgttgttgga tgtttctaag gataatccta
    28681 gaatgattgc ttttttagtg ccacatcaaa acagcgataa accgctttat gagtttgtaa
    28741 ttgctgtgga tgaattagaa aaacggacag gaattgattt cttctacaat ttgcccgatg
    28801 aaattgaaaa taaattagaa aagcaaaccg actataaaat gtggagtttt taacgaagga
    28861 tttaccactc atttacttta ttggcatcta attttagaaa aacaaaaagc aaaatagtaa
    28921 aggcccacaa actggagcct ccataagaaa agaaaggcag tggcactcca atagtcggaa
    28981 ataatttaat aagcatcgtg atatttacaa aaatatgtgt aaataaaatg gaagctacgc
    29041 aatagccata aacacgactg aattttgttt tttgtcgttc agccaaatag atgattctga
    29101 aaagtaaagt aacaaaaagt aaaataacaa cggtacaacc aataaagccc cattcttcac
    29161 caacggtcgt gaaaatataa tcggtatgtt gttcaggcac aaatcctccc ttggtttgag
    29221 ttccctccat aaaacctttt ccaataactc caccggaacc tatggcaatc atcgactgat
    29281 ttaaattata accttcattc tttaaatcga cagtatcact gaataaaaca ttaatcctgt
    29341 ctttctgatg tggttccagt acattttcaa aaacaaaatc aaccgacaat acaaatgatg
    29401 tggcggctac aaatacaatt aaataggcga ggggtattcg gtttattctt ctggctctca
    29461 aataatgcaa aatcataata attacaataa ccacaatgag ataaatgggc tgtattaaaa
    29521 gggctgcaaa aaataaaact actgccaaaa atccggtcca caaataccac tcaggcaaac
    29581 cttcccgatt taaaaccaaa aacaaagaga agaatatcat agcactaccg gcatcgggtt
    29641 gcataagaat tagtaaaacc ggaaatccaa tgatagcaaa ggcaagcagt tgatgattgg
    29701 tgttcttcaa aaaaacctgt gaatcactta agtattttgc taaaagcaag gatgttgccg
    29761 tcttgacaaa ctcagacggt tgaaaaccga atgaaccaaa accataccag ttggtctgac
    29821 cttttacggt tttaccaaat acgaatagtc caagtaagag aacgattcct aaaatgtaaa
    29881 aaacaaaaga aaatttctca taaattttgg catctatggt aagcacgaca ataatgaccg
    29941 gaatggtcaa taaaataaag ataagttgct ttccataata atcaccaaaa tcaaattcag
    30001 taaattcaat aggaatcgaa gtagaaaaaa tatttaacca acccattaac accaaaagac
    30061 catatattag tacggtcgtc caatctaaat tgctggctac actttgattt ttcattattt
    30121 attaattgaa aacggttgtc cgctcaacac tttggcatac tcactttcca aactcgattc
    30181 taacattctt ttttctaaat ctgttcggga aatttttcct ttaatatatt tttcaatcat
    30241 taaactggaa attggtccgg caattcttgc tccccaataa ccgttttcca caaacaccgc
    30301 aatggcaatt ttaggattgt cttttggtgc aaatgccaca aaaatagaat ggtcagtcaa
    30361 ttgaacccgt tgtccgttta tttttgtaaa gttttccgcc gttcctgttt ttccacaaat
    30421 atcaattcca tccactttta atcgtgaagc ggttcctaaa ttgtatacgt caaataatcc
    30481 ttcaattacc ggagggaaat attgtttatc aatcgtggtt tgatgtttgg tcgtaaactt
    30541 tttatcgata gtatgatctt ttatcttttt gatgatatga ggggtataat aatagccttg
    30601 attagcaacg gcacacatca cgttacataa ttgaatgggc gtcattaaaa cttctccctg
    30661 accaatcgaa ttagagataa ttgtagtgct tttccaaccg ccggcggaat aatatttatt
    30721 gtataatttt gaagtaggaa tacttccttt tcgtccgggt ggcaaatcat atcccataaa
    30781 attgcctaac ccaaaactct ttaaatgatt actccaaaca tctactccat cggccggatt
    30841 tggatatttc tgaatggttt tcaaataaat attggcaaaa taggtattac acgatttgta
    30901 tattccatca tgcaattgag aaggtccgga atcgtgacac ttcataaatc gattaccata
    30961 actaaatcct cttcggcaga aataagttgt ttgttcgtca attacttctt cttgtaaagc
    31021 aaccaaagca gtaaggattt taaagggtga gccgggagga tactcagcca gaagacctct
    31081 gtcatatagc ggttttgcga tactatcgtt gtaaagtgcc gtgtaatttt tagaacgcat
    31141 tcttcctacc aaaagagaag ggtcataggt tggggctgaa attaaagcta aaatctcacc
    31201 ggttttcggt tcaattgcta ctattccgcc acgtttgttt tgcatcagca attccccgta
    31261 tttttgcaat tccatgtcaa gcgagagagt aatgtcatcg ccttgcttgg agagagtatc
    31321 aaaaatgcct tctttatacg gaccaatatc gcggttaaac ttatcccgtt gaatatattt
    31381 aacgcctttt tgacctctta aaaagtcttc ataatattcc tcaacacctt gtttcccaat
    31441 caaatcaccg cttttataat aagggttttt ttcaatcatg gcaggactaa cttgtgtgat
    31501 aaagccaaaa acattcgctc ctatattggc ttgataatcg cgtaaggatc gcttttgaat
    31561 ataaaaacct tcaaattttc tgattttctc ttgaaaagca gcatattctt ttttattaag
    31621 ttgaggtaaa aaaacggaag gaaggcgagg actgtaaaca gttgcctttt ttattttttt
    31681 gtcaaattcc tctcgggtaa tgcttaataa atcacagaat tttaaagtgt ctaaattttt
    31741 aatttccctc ggaaccacca taatatcata cgacggctgg ttggcaacca taagttttcc
    31801 ttttcgatca taaatataac cgcgttcggg atattcataa acaattttaa tggcattatt
    31861 ctctgatttc agtttatatt cattgtcaat tatttgcaaa taaaacaacc tcgctaaaac
    31921 aacaagggta gcaaatataa ttaaagaggg tagaatagct tttctcaaat cttcttactg
    31981 ggcttaaaca aataaatgag taggatacaa agggcaagtg taaagatagt actcaaaacc
    32041 gttcgaagca atatattcca gaaaaaacta aagcggaata tttctaataa aaataaaata
    32101 atatgatgcg tcaatacgga aattaaaata aatgaaaagc gttcaggggt taaacgctca
    32161 ttaattttaa cggtttgata ttcataactc aaaccaaaag agaatttaaa aaaggaaggc
    32221 cgtaaataag ctaatatcac acaagcggca gcatgaacgc caccggaatt actaaacatg
    32281 tccatcgtga ttcccaataa aaaactggct agtaaaagac cgtttttatt cccgttaacc
    32341 ggatataaga taataaacaa aatataagga tacggattaa tgaaacccaa aaaattaatt
    32401 ttattgaata tagcgacttg agctaaaatc aataaaatga aacggataat attgacgata
    32461 acggcactac tcatctttat tttgtgcttc taattctatc acttctttcc ggtctttatt
    32521 ttcaatgatg taaacatgac ctaaactcgt catgtcatta aataatttca catcaatcgt
    32581 aaaataattc gttttgttat ccgtataaat tttatgaatg gtgccaatat taatattctc
    32641 aggaaaaatt ttggattgag ccccagtaac aatagtatcg ccttttttaa ctgtggctaa
    32701 tcgtggaacg tcaatcaatt gaacataccc tgtgctttta ccattccaag ttaacgtacc
    32761 gaaatgactg gattttttaa tttttgcatt gatttgtgat ttcacattca acacgctgat
    32821 taccgtagca taattttttg aagtatgttc aacaataccg atgatacctt tgctgttcat
    32881 cacgcccata tcgggttgaa tgccatggac acttcctgaa ttaatggtaa ggaaattttc
    32941 atgaacatta taagtattgt taatgatttt agattggaat actttgtaat ttccaaagct
    33001 gctcaataat tccttgtcaa ttacttttgt ggtgtcttta aaattgaaaa gtaagctttt
    33061 taaacgggca ttttcattgg ctaatccttc gttctccgtt tttaaattca aatattcgtt
    33121 cacggaatta atgcgttcat acacgcctcc tgtcacaaaa ttggcagaag aaataacctg
    33181 actgcgatga aaagaatgtg attgtatcgt caaaaccaac gaaaccacta aaagcagcaa
    33241 aaacagtaac cgtgtactgt ttttaaaaat ataaacaaat agctgctgca tagttcaatg
    33301 attcagttac gtaattccgt ttagtaaaaa agagcagtat aaaccgtatt atttgattaa
    33361 aatacttctg aatcgggtaa tgtttttcaa cgcaattccg gtacctctaa caacagctcg
    33421 caacggatct tctgcaatat aaacaggcaa atccgttttt aacgaaattc gtttgtctaa
    33481 gccacgtaac atcgaaccac ctccggctaa ataaatccct gtattataaa tatcggctgc
    33541 taattccggt ggagtttgtg ataaggtttc cattacagca tcttcaattc gttgaatcga
    33601 tttgtctaac gcttttgcaa tttcacgata agaaacatca acctgtttcg gtttcccggt
    33661 taataagtca cgtccttgca cagacatttc atccggtgga gtatctaatt cttcaatggc
    33721 agcaccaatt tcaattttta ttttttcagc agtactttct ccaacaaata agttgtgttg
    33781 cgtacgcata tagtaaacga tatcattagt aaaaacgtca ccggcaattt tcacggactt
    33841 gtcacaaaca attcctccta aagcaataac ggcaatttca gttgttcctc ctccgatatc
    33901 cacaatcatg tttccttttg gttgcatgat gtcaacgcca ataccgatag cggcagccat
    33961 tggctcatga atcaaataaa cttcttttcc gtttacgcgt tcacaggatt cctttaccgc
    34021 acgcatctct acttcggtaa ttccggatgg gatacaaacc accatgcgta aagccggcgt
    34081 aaacaaacgt ttctttaaag caggaatact tttgataaac atgctgatca ttttttctga
    34141 agcatcaaaa tcagcaataa ccccatcttt caatgggcga atggtcttta tgttttcatg
    34201 cgttttacct tgcatcaaat tagcttcttt tcctacagct ataatcttac ccgatatcct
    34261 atcacgagca acaatcgacg gggagtcaat cactacttta tcattatgaa ttatcaaagt
    34321 gtttgcggtt ccaaggtcaa tggcgatgtc ctcggtcatg aaatcaaaaa atcccatacg
    34381 tttgtttagg gttaaatagg tttataaaat tctaacgtac aaaggtaaaa aaattaatgt
    34441 ttaaaatgac gtgttcctgt aaataccatt gcaattttat tttcgttgca atagctgata
    34501 ctcaactcat ctttaattga tccaccgggc tgaataacag ccgtaattcc tgcatgatga
    34561 gcaatttcta cacaatccgg aaaggggaaa aatgcatcac ttgccatcac ggctcctttc
    34621 aaatcaaaac caaatgaagt tgctttttct atggcttgtt tcaaagcatc cacgcgagac
    34681 gtttgtccgg ttccggaagc caataattgt ccgttttttg caaacactat cgtattcgat
    34741 ttggtgtttt tacaaatttt ggaagcaaat agtaaatctt caatttcctg atcagtaggg
    34801 gttgtcaagg taacggtttt taatccttgt ttattgtctg tcaagttgtt tttatcttga
    34861 actaacaatc cgtttaaaca agttctcact tgcttattgg gtaaggcaac atcatgttgc
    34921 actaatataa tacggttttt cttttcttct aaaagggcaa gagctttgtc atcataggcg
    34981 ggagcaatca ctacttcaca aaacaagtga ttgatttctt ttgcagtggc ttcatcaatt
    35041 ttcccgttgg caatcaaaac gcctccaaaa gcagaggttg gatcacctgc caaagcagct
    35101 aaataagctt cttgcatcgt gcttcgggtg gcaattccac aagcattatt atgttttaaa
    35161 atagcaaaag tagggtcatt gtctttaaat tctaaaatca aattcactgc agcatcaaca
    35221 tccaataaat tattatacga aagttctttg ccgtgtaatt tggtaaacat gctgtcaaag
    35281 tcgccaaaga aaaacccttt ttgatgcgga ttttcaccat atcgaagcac ttgaccgtca
    35341 gcaatactgg ctttgtatat cgttacatcg gtgttgaaat aagtaaaaat agccgtatca
    35401 taatgagagg acacatggaa ggctttggtg gctaacaaac gtctttgctc taaagtggtc
    35461 gctccgtttt gagtagaaat taatgctaat aaataagcgt attcgttcac agaagcaaca
    35521 ataacggtat ccttgaaatt cttggcagca gcacgaatca atgaaatacc accaatatca
    35581 atcttttcaa taatatccgt ttcagaggca ccggaagcta ccgttttctc aaacggatat
    35641 aaatctacaa tcaccaaatc caattgcgga atttcaaagg ctttcatttc ttccacatca
    35701 cttggattat cctgacggtt caaaattccg ccaaaaactt tcggatgtag tgtttttact
    35761 cgtccaccta aaatggatgg ataggaggta acctcttcta caggaactac cggaatacct
    35821 aagtttttaa taaattcttc ggttcctccc gtagaataaa gaataacatt ttgttggtgt
    35881 aattgcttga caataggttc taaacccgtt ttgtcaaaca ccgaaattaa cgctgattga
    35941 atggtttttg tcgtattcat tgtgttgttg tttagaaggg gcaaaagtac tatttgtagg
    36001 cctaactttc aaacgatttg catattactt tttatccttt ttttgttcca ttttttgtca
    36061 atccgcaaaa gacgaagtcg aaataggatt ctcctgcaaa gcaggacaaa aaaccaatca
    36121 ttttctgtaa caatttccca atttaaacta ccaatcacta tatttacatt tacaaaacaa
    36181 aacaatgctg ctctaccttc gtttacttaa agaaagtttt agtttcgcca tgaatgcctt
    36241 gcgtaacaat aaacttcgta cttttttgtc gttactcgga gtaaccattg gaattttttc
    36301 tatcatcgca gtattggcag cggtggattc cttagaccaa aaaattaaag ccgacttagg
    36361 cgatttagat aaaaataccg tctatttaat gcgtttttcc tttggtcctt ccgaaattcc
    36421 gcgttggaaa agagaacaat tcccggatgt gacctttgaa gaatttgact acctgaaaaa
    36481 atccctgaat ggagtagata aaatggcttt taatttattc acgggaaatg aaaatgtaaa
    36541 atttgaagag cgaacggtaa gttccgttcg tgtcacaccc acaaccgaag aatttgaagg
    36601 tattgatgga ataaaaatag agcacggtcg tttttttaat gaatcagaat ctaattccgg
    36661 taccgccgtt gtcatcttag gatacgaagt agcaaacgga ttgttcgact ctgctgaaca
    36721 agcgaatgga aaaaaaataa gaatgtacgg gaaacgtttt acggtcattg gagtaacaga
    36781 aaaacaaggt ttctctactt ttggcgacag ccgcgataat gccgtgttca tcccgatgaa
    36841 tttcatccga cgtctatatg gcgaaaacaa cgaaaacata acaccggcca tcttaatcaa
    36901 acctgaaaaa ggaattgaca tggaagcttt caaaggtgaa atgactcaaa aattaagaaa
    36961 ctacagaggg ttaaaatcct ctgaaataga caactttttc attaatgtgc tttccggatt
    37021 tacagacatg attgataata tcgtggggca aatgaatgtt gtcggatgga ttatctccgg
    37081 attttccttg ttagtaggtg gtttcggaat tgcaaatatc atgttcgttt ccgtgaaaga
    37141 acgtactaac ttgattggta ttcaaaaatc actgggagcg aaaaacagat tcatcttatt
    37201 tcagtttctg ttcgaagccg taatcttatc cgtaatcgga ggaatcattg ggctagtaat
    37261 ggtgtggtta attgcaatga ttatgacaag cgtactcgat ttcgagttca ttttgagttt
    37321 tggaaacatg atgttagggg ctggtttagc cggaatcatc ggactcattt caggaatcat
    37381 ccctgcgatt tcagcttcaa aattagaccc ggtagaagcc attcgaagtg gaatgtagag
    37441 tatacggcgg agagttttat agaattctac cctaaaccca caaccatttt atcatccttt
    37501 ttcttcacgc tttctttccg taaaaataag aggttttgct ttctacgtca tcataggtca
    37561 ccggttgctt gtctttatgg aactcaaaat aactcaataa cccatagtac ctcccgactt
    37621 tgcggaattt ttttaatata tttgataaaa gataattcaa atcaaacgtt cttaaaatac
    37681 tggaagagaa tcattattac cctcccgacc attcgggatg gtttaaaaca caccaattac
    37741 aacagcgaca taagaaagta caccaaagaa acggatccta tcactcaact tttaaaactt
    37801 aaaatcttac cgcctactga tggaaaaccg ttggagtata agtataaata caatgggaag
    37861 gagtggcaag acgagttggg gcttgatgtt tatgattacg gaggaagagt ttatgaccca
    37921 gctgctccac gattttggca aattgaccca atggcagagg atagggagtg gctatcacca
    37981 tataattacg ttcaaaacaa tcctattatc agaattgacc ccgatggttt attagatgat
    38041 ttggcagttg gcgatactga cccaccaacc aaaaaagcag ataatacttc tcgtttatct
    38101 ggttggtgta aatcgtcagc aaaaagtgta ctgatttaga caaaaactaa gagagtgtt
//
LOCUS       DAOTCW010000007        36770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_562_length_36770_cov_40.962005, whole genome shotgun sequence.
ACCESSION   DAOTCW010000007 DAOTCW010000000
VERSION     DAOTCW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 36770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36770
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_562_length_36770_cov_40.962005"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1229
                     /locus_tag="P5335_01190"
     CDS             156..1229
                     /locus_tag="P5335_01190"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRZ73524.1"
                     /translation="MFVFLVCFITRTKILCLSFPIRLLNNQGVTKKIPSFLYITMDEA
                     MKYNRYLKNTPNVSAIGLGAWQLGSNSGWQGMTEKEALELVAKALGYGINFFDTAPNY
                     GHGSGETRLGKALKGVDRDRIVINTKFGHTDSGTTNYASNYIRESLEGSLRRLQVDYV
                     DSLLFHNPPLAYLDGTKNDHYDVLERLVEEGKIKAYGASLDRFEEMQLLMNTTNAQVI
                     EAFFNILHQDAARAFELAKEKEVRIIAKIPLDSGWLSGKYTAESTFDDIRSRWSRADI
                     ATRAHLVTRVKELVGAEENLASVALSFCLGYDAISTVIPGNVSLAQLASNVEPVHRPL
                     SKELIAQLEAFYLNEVEPLRLPW"
     gene            1398..4928
                     /locus_tag="P5335_01195"
     CDS             1398..4928
                     /locus_tag="P5335_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ73525.1"
                     /translation="MKKILLSFFIVFPAFVFAQIGFEQHVVIDNTFGTPYPQRVAAGD
                     IDGDTYSDVLVGGSGKLAWHKNTNGLGTFSTAKTVAPGLSSFNSVAIADMDNDTYPDV
                     VYSIWSGNLAKVYWQKNTDGLGTFGAAIQINTNNNYLMKLEVIDFDNDGDNDVVLNSF
                     DNVILLKNDGAGVFTTSNLLGANNSFHIVDVTGDGLADVIRVAGYDLVAFKQNVDGTL
                     TLLDNMDGFAQTTLIFSADIDNDGDTDILTIFENGGAQRRIKWYQNTNGLGTFAANQV
                     LVTLPNLAVTSSNDTKGLQFSDFDGDGKLDIMLSESSTNKLCWYKNLTGTTFGTEQII
                     TTDASNVREAVAVDVNSDGDDDIVSVSYDDGDVAWYKNTNGLGAFSAANMVSYYAYSV
                     NHIDVGDFNNDGTPDILSTSHGDKKVAWYKNVDGLGSFSQPQALISKSINGTRNGYAK
                     DMDGDGDLDALYTYYLDDNVDVFNIRWKENNGSGIFTTEHVVYVGIPDLYAITPCDID
                     ADGDTDIVAVFNLNNLMVLKNNGNGTFAPQQLFNFPVGSLGYIVVEDVDGDGDLDVIS
                     SGSNKFAWYENTNGQGDLSVEHLIPLSNNSSRVLNTADLDGDGDQDILFINRTPNKIG
                     WFENTDGAGTFGPEIILATVTKPLSILAVDLDGDGDQDIVCDAEQGFRLQLFENNGNA
                     TFSAGFEITSQIGRVTSLVAEDMDADGDLDLITSSYDDDQVAWFENLGSFRNTLQGTV
                     RIDTDSNGCNSTDVVVPNVLVTTDDGANTFSTFTGASGQYVLYANEGNYTTSVTTPLP
                     NYPPNPNSQVWSFEGVNQTQVVDFCLEPSTLFEDLEVSLVPLNLARPGFVSKYRIHIK
                     NTGTNPMNGVLTFTYNPVKFSFITSSTATSSQTANSLSFTFTNLNAFQTKIIDLEFQV
                     ATIPTTTIGESVLFTATLNENTNDVTPENNTFLFHQLLVGAYDPNDIQVVEGPEIAFE
                     DVDNYLHYIIRFQNTGNFYAQKVVVTNELDDKLDWTTFQLESYSHPNRVELLNGTSVD
                     FIFNAIYLPSVNQDEEGSQGYIAYKIKPKTSVGLGDVISNTASIYFDYNPAIITNTVT
                     TTVVETVLSSPSFETESVTLYPNPTSGWLTINSKAIISKIEVYNQLGQQVLQSDTSSS
                     IDLSALSQGVYFIRFVDSNEQVLIKKVIKQ"
     assembly_gap    5364..5463
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5464..5867)
                     /locus_tag="P5335_01200"
     CDS             complement(<5464..5867)
                     /locus_tag="P5335_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRZ73526.1"
                     /translation="MFLLASIALTLSPGPDILYVLTQSISNGKKYGIATALGLVSGIL
                     VHTTLIALGVSAVIKESEIIFTVIKIFGACYLLWIAYQVYKAPASITLNTSAKPQKSI
                     LALVKQGFIMNVLNPKVTLFFLAFFPGFIDEKA"
     gene            complement(5887..6906)
                     /locus_tag="P5335_01205"
     CDS             complement(5887..6906)
                     /locus_tag="P5335_01205"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRZ73527.1"
                     /translation="MYKLLIRPILFCFDPEQVHYFTFSLIRFLHKIPGVGKLFQAMYE
                     VKNPKLEREVFGLKFKNPVGLAAGFDKDAKLYKELSNFGFGFIEIGTLTPKPQEGNPK
                     KRLFRLKEDAAIINRMGFNNGGVLEAVERLKKNNGVLIGGNIGKNKNTPNETATADYE
                     SCFEALFPHVDYFVVNVSSPNTPNLRALQDKEPLTQLLQTLQNRNLAKPKQKPILLKI
                     APDLMNEQLLDIIDIVKETQIAGVIATNTTLSREGLHSENKTEMGGLSGKPLTKRSTE
                     VIRFLSEKSNKAFPIIGVGGIHSADEAIEKLNAGASLIQLYTGFIYEGPGLIKEINNR
                     ILKEV"
     gene            complement(6944..8068)
                     /locus_tag="P5335_01210"
     CDS             complement(6944..8068)
                     /locus_tag="P5335_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRZ73528.1"
                     /translation="MNKIYLDNASTTAIHPEVIQEMTRVMQSEYGNPSSSHSFGRGAK
                     AVLESARKSIAKQLNCTAQEIIFTSSGTEANNWILQNAVKKIGIKRIITSKMEHHAVV
                     YPILALQKEFGIEVEFLKVLPNGQVDYLQLTALLDSHTPTLVSLMHVNNETGVVLDIE
                     RVGELCKLHKAYFHSDTVQSMGKTELDLQQLNIDFLVASGHKFHGPKGVGFAFIKKNT
                     PIAALLVGGEQEKGLRAGTEAVHQIAGIAKALEFAYEGLESQRDTIFELKKYTIEKLT
                     AQFPSVKFNGGLETFYTILNVQLPLAEDKTSIILFELDMKGIAVSRGSACQSGSIKPS
                     HVLVEFLSESELKKPSLRLSFNQHNTKEEIDYFVSCLAAM"
     gene            complement(8189..10288)
                     /locus_tag="P5335_01215"
     CDS             complement(8189..10288)
                     /locus_tag="P5335_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HRZ73529.1"
                     /translation="MKNSFLISIFLLSMITFGQKLQSPDKQVELSVGVNKIGRPFYSL
                     NYKTKPVIQTGFLGVVLKNTSTLAAEFEIVNSTVSAVNENWKPVLGEQKEIQNSYNQL
                     VLELFQKSSKRKMNIVFRLFNEGLAFRYEFPAQENLNYFVISDEVTQFALVGDHKAFW
                     IPGDYDSQEYVYNETKLSQVDVSKLDMNNGIGMKGPMANSRIQSPVMMKSADGLYINI
                     FEAAVVNYPIMHLELDTTTFLMQSHLVPNAIGDKAYLQAPANSPWRTIMVSDDARKIL
                     ASKMTLNLNEPSKIEDTSWIKPMKYVGIWWEMHVGKATWDYAGSQNAQMAQDAELKPT
                     GKHGATTENTKRYIDFAAKHGFDGVLVEGWNIGWEDWFGNWKENVFDFVTAYPDFNVE
                     EVQKYAESKGIKMIMHHETSGSVANYERRLDRAFSFMKKHNYNAVKSGYVGKIIPRGE
                     WHDGQSMVNHFNWVVERAAEYKIMVNSHESSRPTGVHRTYPNYIAAEAARGNEFNAWS
                     NGNPPMHETILPFTRFLGGPMDYTPGIFEIKMSHYDATKKEQVHTTLAKQLALYVTIY
                     SPLQMAADLPENYERYPDAFQFIKDVEVDWDETKIIEAEPGDYLTIARKTKGKESWFL
                     GAITDENARNSEITLDFLTPGKKYKATIYQDGKDAHWEKNPKAYTIKTMEVTSKTKLK
                     LILAAGGGTAISFIPLN"
     gene            10410..11408
                     /locus_tag="P5335_01220"
     CDS             10410..11408
                     /locus_tag="P5335_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73530.1"
                     /translation="MKRFLGFLVLLLTLNACDDGDFDIETFNFTDVNTNQCNSGANGF
                     FIYKINNTEVLLVQIPESNFINEITVPGTPRTVNIGGANKVIYRIYDGTVTTGDICNT
                     IPPATPVVTEEWNALAGTIEIVTTVNKTVDETLNSSIITGYTHNIILRNVNFQKGNGK
                     EQLFESLTFGNYITSATAMANFDGFAIKNCATDYNFLYKISGSQALAFAVDATLFQNE
                     VTPLDSPRTALIDDINQLELRYYNDNINDAFACTTPPLSYPVLEQKWVSEDGITGVSG
                     MIEVTTTEEYLIPTDNQSPLVGYRQKVTLKKVTMVRNGVSFKLGDVYEYGEFVTPL"
     gene            complement(11403..11957)
                     /locus_tag="P5335_01225"
     CDS             complement(11403..11957)
                     /locus_tag="P5335_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRZ73531.1"
                     /translation="MFNIGDKVAALDEDISGVVVSFKNNEVEIETTDGFLMTYFVNEL
                     IKINITSELSSFISTDKLYKAIQEKTEPTKRSFVKEKKSRKDDFVLEVDLHIEKLVPS
                     KRGMNNYDILNLQMETAKRQLEFAIKNKMPKVVFIHGVGEGVLKAELDFLLGRYENIK
                     FQDADYQKYGLGATEVYFIQKKML"
     gene            complement(11950..12132)
                     /locus_tag="P5335_01230"
     CDS             complement(11950..12132)
                     /locus_tag="P5335_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73532.1"
                     /translation="MKKVIIPIMLIAVFFAFYTPKETNYKVYIKVAAIVILMIGVMKL
                     MQKVPSKNQEKEEEDV"
     gene            12258..12536
                     /locus_tag="P5335_01235"
     CDS             12258..12536
                     /locus_tag="P5335_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HRZ73533.1"
                     /translation="MEEYMLPCLSKKLFGIDCLGCGIQRAVVLLFKGEFAAAFYMYPA
                     IYTLLLFFAFIGIRFIDKSRNYHKIIMLFGSVTAVIMVVSYLYKVIVY"
     gene            complement(12583..13863)
                     /gene="rocD"
                     /locus_tag="P5335_01240"
     CDS             complement(12583..13863)
                     /gene="rocD"
                     /locus_tag="P5335_01240"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRZ73534.1"
                     /translation="MSVLEKINSKEFIALEDRFGAHNYHPLPVVLSRGEGVYVWDVDG
                     KKYYDFLSAYSAVNQGHCHPKIVNALIQQAQTLTLTSRAFYNDKLGKYEKYITELFGF
                     DKILPMNTGAEAVETAIKICRKWAYEKKGIDEHEAIIVVCDNNFHGRTTTIISFSNDE
                     NARKNFGPYTGGFIKIPYDDIEAFQFVLENNKNIAGFLVEPIQGEAGVYVPAENYLEQ
                     VKELCDQYNVLFIADEVQTGIARTGSLLAVCGNCTCENNCERQDTYAKPDILILGKAV
                     SGGVYPVSAVLADDNIMHVIKPGQHGSTFGGNPLAAAVAKAALEVVEEENLIQNARAL
                     GKLFRAKLNEYIENSSIVSLVRGKGLLNAIVINDTEDSSTAWDICIKLAENGLLAKPT
                     HGNIIRFAPPLVMNEEQLLDCVSIIIKTLKEFEK"
     gene            14074..14511
                     /locus_tag="P5335_01245"
     CDS             14074..14511
                     /locus_tag="P5335_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73535.1"
                     /translation="MKKILFLLLSFTLVGISSCSSDDDKSDVNYVTAKFNNVEIKFNI
                     ISVDKIDYDGYSDIIVTATTNSDPTKVITIASEYGVTGQNEIWGFDYETADEYFVQDA
                     ANFTSTLTTNSDGKYIGTFSGSLVEDTNGNVMVLTNGAFNIAY"
     gene            14621..16033
                     /gene="rlmD"
                     /locus_tag="P5335_01250"
     CDS             14621..16033
                     /gene="rlmD"
                     /locus_tag="P5335_01250"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRZ73536.1"
                     /translation="MGRKNTDKIIFKEVTVLDAGAKGVSVAKAEDGRVIFISNVVPGD
                     IVDVQTFKKRKSYFEGKAIYFHKLSPHRVKPECEHFGVCGGCKWQNMNYNQQLFYKNN
                     EVYNNLKRIGKIELPDFEPILGSAKQFFYRNKMEFSFSDSRWLTENEIQQEDTIENRN
                     ALGFHIPKMWDKILDINKCHLQQDPSNAIRNAIKSFATENNLPFFNTRHQEGLLRTLM
                     IRTTSIGEIMVLIQFYKEDKTQRELVLNFLKDKFSEITSLLYVINGKANDTLYDQDIK
                     LFSGRDYILEEMEGLQFSINAKSFYQTNSDQAYELYKITREFAGLTGNELVYDLYTGT
                     GTIAQFVSKKAKKVIGVESVPEAIADAKENAKRNNITNCEFYVGDMKEVFNADFIAKH
                     GKPDVIITDPPRDGMHATVVEQIMQIAPEKVVYVSCNSATQARDLALMDSLYKVTRVR
                     PVDMFPQTHHVENVVLLEKK"
     gene            16034..16531
                     /locus_tag="P5335_01255"
     CDS             16034..16531
                     /locus_tag="P5335_01255"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HRZ73537.1"
                     /translation="MKRFLFFVVLLFLFQGCEKDDICTPETPTTPRLIIEFYDSVNTS
                     VKKVVANLKAEGEGADTALTFDLVSTIELPLKTNEDFTQYKLTINSTSATEANMDSIR
                     INYTRNDIYISRACGFKTNFDLNLFNPIENSNPVGDTEFWIDTIEIIKNKIETEDDVH
                     IKMYF"
     gene            16506..17231
                     /locus_tag="P5335_01260"
     CDS             16506..17231
                     /locus_tag="P5335_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HRZ73538.1"
                     /translation="MSISKCIFSIGLILVSISIGAQETKKDSIPLKTERYGVRVGIDL
                     FKFGKSFFDENYKGLELVGDYRLTKNYYLAGELGNENKTTQDDRLNFTTKGSYFKVGF
                     DYNVYENWLDMENLIYIGLRVGASTHSQEINTYKVYNPNPYFGEAPEVISGEKFDGLS
                     AQWGEFLIGVKAELFSNLYAGFSFRLNYLINTKEPDGFENLYIPGFNRTYDGTFGVGF
                     NYTISYFIPIYKKKIEPKKEVKK"
     gene            complement(17233..17463)
                     /locus_tag="P5335_01265"
     CDS             complement(17233..17463)
                     /locus_tag="P5335_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73539.1"
                     /translation="MNIKTKVFIYQLLSFAAFFIPLRFAISYFSFLKGIWIPFLAFVI
                     TLILAPKFQVITTKEGSKIFMKWIFSKEIKEL"
     gene            17605..18894
                     /gene="thrC"
                     /locus_tag="P5335_01270"
     CDS             17605..18894
                     /gene="thrC"
                     /locus_tag="P5335_01270"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRZ73540.1"
                     /translation="MLYKSLNLKAPKVTFEQAVTQGLAPDKGLYFPDTIIPLEHSFFE
                     TIATRSNEEIAHTVIKQFIGSEIEENDLRKIISETLCFDFPLTKIEENIYSLELYHGP
                     TMAFKDVGARFMARCLAYFNKNKPNQKNTILVATSGDTGGAVANGFLNVQGVEVVILY
                     PSGKVSDIQERQLTTLGQNITALEVDSVFDDCQDMVKRAFLDPELAHKNLTSANSINV
                     ARWLPQLFYFFFAYKELKKMNLPIIISVPSGNFGNICAGILAKKLGLPIHHFVAATNI
                     NDTIPRFMLNGMYNPKPSKATLSNAMDVGNPSNFIRIQELYQHDFSSFKNDFSSYTFS
                     DEETLVALKDLYTKNQYIAEPHGAIGYLGLKEEMKKHGKSVGVFLETAHPIKFLDTVE
                     PALEICLPIPTQIESVMNKEKKSIKIKTYTDLKKYLS"
     gene            19009..>19517
                     /locus_tag="P5335_01275"
     CDS             19009..>19517
                     /locus_tag="P5335_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRZ73541.1"
                     /translation="MKSKSNIITFIFIITALFLALISHLVFRNNLKNNVENFNSYHFI
                     VDACLIIGAGLLIRFVIARNDRRNIGILKQLQTINTEIKDSNERYDIVAKATSDTIWD
                     WKIQTDEFVWNKGIQGVFGYKKEDVGNSSNWWFDRIHPEDSLKMSVKLYSFLEQKTER
                     WQDEYRFKCA"
     assembly_gap    19518..19617
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19618..20448
                     /locus_tag="P5335_01280"
     CDS             <19618..20448
                     /locus_tag="P5335_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRZ73542.1"
                     /translation="KKNGDEYWVNFSMIPVTNKEGEHSHWISIQRDVTKEKNQERERE
                     QLIQELTRNNKDLKQFSYITSHNFRAPLSNLTGLLNLLDDVPIDNVELQEILNGFKRS
                     THLLNETIDDLVKVVIIKDNPSIQKEIVSIKEIFENVFFQLNNQINLYKPIINIDIED
                     NLSITINKAYLESIVLNLMSNALKYRSLTKTPKISIIAVEKENSVNLIIKDNGIGIDL
                     GKNSDKIFGLYQRFHNYPDSKGLGLYLVKSQIEAMGGSIEVESQVNKGTQFSLTFKKV
                     "
     gene            20448..20858
                     /locus_tag="P5335_01285"
     CDS             20448..20858
                     /locus_tag="P5335_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRZ73543.1"
                     /translation="MLKSILCVDDDPITLMLCKKVIVKAEFSEEIIFANNGQEAYDLF
                     QSFVVKKDRDAIFQFPQLIFLDLNMPVMNGWEFLDLFKENLSKEFSQTKVIILSSTID
                     PSDYQKAKQYEMVSHFLSKPITVEMLQNLKPLYS"
     gene            complement(20913..21164)
                     /gene="rpsT"
                     /locus_tag="P5335_01290"
     CDS             complement(20913..21164)
                     /gene="rpsT"
                     /locus_tag="P5335_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRZ73544.1"
                     /translation="MANHKSALKRIRSNEKRRVLNKYQHKTTRNAIKALRVSTDKSDA
                     SAKLSSVISMIDKLAKKNIIHDNKASNLKSKLTKHVAKL"
     gene            complement(21199..21270)
                     /locus_tag="P5335_01295"
     tRNA            complement(21199..21270)
                     /locus_tag="P5335_01295"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21235..21237),aa:Glu,seq:ttc)
     gene            complement(21316..21387)
                     /locus_tag="P5335_01300"
     tRNA            complement(21316..21387)
                     /locus_tag="P5335_01300"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21352..21354),aa:Glu,seq:ttc)
     gene            complement(21464..>22231)
                     /locus_tag="P5335_01305"
     CDS             complement(21464..>22231)
                     /locus_tag="P5335_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRZ73545.1"
                     /translation="QAGTSHFLGQNFAKAFDVKFANAEGTQEFVWGTSWGVSTRLMGA
                     LVMTHSDDNGLVLPPNLAPIQVVIVPIYKSDEQLEAISTEVQNVVDALKKLNISVKFD
                     NRTTQKPGFKFNEYELKGVPVRIAIGPKDLENGTYEVARRDTLTKEIVSKEGIEVYIQ
                     NLLETIQTNLFDRAFEYRNTHITEVDTFEEFKNLLDSKGGFFAAHWDGTPETEEKIKE
                     LTKATIRCIPLNRMEEPGMCMFSGKPSVGRVLFAKAY"
     assembly_gap    22232..22331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22332..23097)
                     /locus_tag="P5335_01310"
     CDS             complement(<22332..23097)
                     /locus_tag="P5335_01310"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963521.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRZ73546.1"
                     /translation="MSKNLTKRAEDYSKWYNELVVKADLAENSAVRGCMVIKPYGYAI
                     WEKMQAELDKKFKETGHSNAYFPLFVPKSMFEAEEKNAEGFAKECAIVTHYRLKNDPD
                     RKGKLMVDPNAKLEEELIVRPTSEAIIWSTYKNWIQSYRDLPLLINQWANVVRWEMRT
                     RLFLRTAEFLWQEGHTAHATQAEAIAESVQMMNVYADFAENFMAIPVVKGIKTESERF
                     AGAVETYCIEALMQDGKALQAGTSHFLGQNFAKAFDV"
     gene            23197..24168
                     /locus_tag="P5335_01315"
     CDS             23197..24168
                     /locus_tag="P5335_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73547.1"
                     /translation="MKTKYLTSFKILSLLLTSTLGVLLVSCGSTQNSSYYDNDGIYSG
                     SEKSKSKEAEQVAQNNANLAYQNYFKSKQLNAEEEIFTDVENYSSVTDSTQTTAESYT
                     SNASWGSNPTSISVNVYDSGWGFNYWNSWYAPYWGFNTWYGPYWGVGFYPWYGAYWGW
                     GYPYYGYGYGYGYGSYYNGYAYNSGRRGGNYYTNGYRGSSAYSSRTNGYRGSSAYRSQ
                     YSTRNYTNSGTRFGTTRTGTRGYNTNAVNSSNPRSNQYNTSGTRNNTRNYGNTPSTGN
                     NTYTPRSSSSSSSSTSGTRGGGYSGGSSGGGGRSGGGGGGGRSGGRG"
     gene            24209..25690
                     /locus_tag="P5335_01320"
     CDS             24209..25690
                     /locus_tag="P5335_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRZ73548.1"
                     /translation="MKKILLFVVGFASLTIQSQEIQDAVRYSLDDMTGTARFRAMSGA
                     FGALGGDLSAINMNPASSVIFNTNQVGFTFSNHSKNNESTYFGTYTEQCDSSFDMNQF
                     GSVFVFHNNDANWKKFAFAINYENENRLDNSVFSAGVNPTTSIDSYFLNYANGIPLGT
                     LQSNLYALNYGEQQAFFGYNGYIISPVTENLSNTQYVSEVPAGGNYYQDNYVESYGFN
                     GKVAFNLATQYKDFLSFGINLNAHFVDFNRASSFYEKNTNNLENGVQDVLFENFLYTY
                     GSGFSVQVGTIFKATQNLRFGASYESPTWYNLNDEFTQRLYATNRVNGVTFNDIVDPR
                     ILNIYPSYEVQTPEKWSASAAYIFGKSGLISFDYTLKNYSHTKLKPENDYVFRNLNTE
                     MANILNNANEFRVGAEYKIKELSIRGGYRFEESPYKNGKTLGDLTSFSGGLGYNFGYF
                     KLDFAYSYSQRKSQEGFFTTGLTSPAFINTITNSFTTSFIFEL"
     gene            25803..27104
                     /gene="rimO"
                     /locus_tag="P5335_01325"
     CDS             25803..27104
                     /gene="rimO"
                     /locus_tag="P5335_01325"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRZ73549.1"
                     /translation="MRTKSLKKNKINVITLGCSKNVYDSEVLMGQLKASGKDVVHEQE
                     GNIVVINTCGFIDNAKEESVNTILEYVDKKEQGLVDKVFVTGCLSERYKPDLEKEIPN
                     VDQYFGTSDLPQLLKVLGADYKHELLGERLTTTPKNYAYLKISEGCDRPCSFCAIPLM
                     RGKHVSQTIEKLVSETEKLAKNGVKELILIAQDLTYYGLDIYKKRNLAELLENLVKVE
                     GIEWIRLHYAFPTGFPLDVLELMKREPKICNYIDIPLQHISDSVLKSMRRGTTQEKTT
                     SLLKEFRKTVPDMAIRTTLIVGYPGEKEEDFQILKDWVQEMRFERLGCFAYSHEENTH
                     AYLLEDDVPSEVKQQRAAEIMDIQSQISWDLNQEKIGQTFKCIIDRKEGPYFIGRTEF
                     DSPDVDNEVLIDASKFYVKVGDFALIKINDATEFDLYGEPI"
     gene            27265..27501
                     /locus_tag="P5335_01330"
     CDS             27265..27501
                     /locus_tag="P5335_01330"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HRZ73550.1"
                     /translation="MITTHPIVQEFPEYLDKIKDLYHHNEEFQVLLGSYHSLDKEIFE
                     IEKGEIPATDDELTHKRKDRVFLKDEIYNYLKNN"
     gene            complement(27539..29737)
                     /locus_tag="P5335_01335"
     CDS             complement(27539..29737)
                     /locus_tag="P5335_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRZ73551.1"
                     /translation="MKDLFLITPPFTQLNTPYPATAYLKGFLNTKNISSFQMDLGIEV
                     ILEIFSKKGLEKMFQFSSSRFQVDENCQRIYTLNQDYIQTIDAVIDFLQGKNPTLARQ
                     ICSGNFLPEASRFEQLDDMEYAFGTMGMQDKAKHLATLYLEDLSDYIVACVDENFGFS
                     RYAERLGRSANSFDELYYQLNQKSTYIDEITLSVLNQKLTDIQPKLVCFSVPFPGNLY
                     SAFRCAHFIKTNFPTIKTAMGGGFPNTELRDLKDKRVFEFFDFITLDDGELPIELLHH
                     SVCHSDEGGISSIQFKRTFLLEKNEVVYKNNSTQPDYKQSEVGTPDYSDLQLGNYISV
                     IEIANPMHSLWSDGRWNKLTMAHGCYWGKCTFCDISLDYIKLYEPIAAKLLVDRMEEL
                     ITQTGENGFHFVDEAAPPALMREVALEILRRKITVSWWTNIRFEKSFTADLCQLLKAS
                     GCIAVSGGLEVASDRLLELIKKGVTVEQVAQVTRNLTESGIMVHAYLMYGYPTQTIQE
                     TIDSLEMVRQLFELGILQSGFWHQFAMTAHSPVGMFPEEFGVIPQQNDITFANNDILF
                     TDKTGINHDKFSFGLKKSLFNFMHGICFEYELQEWFDFKIPKTKIAPDYIISCLEKEE
                     SFNTKPSTKIVWIGARPSNELFTKSKKGKSWEMVKLIFHSKTEAVEISLEKEKGIWFA
                     EMLEKISISGDKTITFSQIKSDFELHFEDFELFWYSKQINTLRSIGLLTL"
     gene            29854..31458
                     /gene="bshC"
                     /locus_tag="P5335_01340"
     CDS             29854..31458
                     /gene="bshC"
                     /locus_tag="P5335_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HRZ73552.1"
                     /translation="MPTDCISYQQSGYFSALINDYLNEKPELKALYHRFPNLENFKLQ
                     LEEKRNNFNYKDNGKLKREDLVSVLEKQYQSIVASETTLNNIKLLNNLNTFTITTGHQ
                     LNLFTGPLYFLYKIVSTINLCKELKSEFPEHDFVPIYWMATEDHDFEEINYFNFKHKK
                     ISWNRESFGPVGRLSSDGLEAVFAVFESEIGLGKNAEGLKNLFKKAYLEHTNLADATR
                     FLANELFSKEGLVIIDADKSDLKKSFIPYIKNEVLNQTAFAKVNETSPLLESYSVQVN
                     PREINLFYIENNLRERIVFENGSYAVLNTKMTFSEEEILKEVDNHPEKFSPNVLLRPL
                     YQEVILPNLCYIGGGGELAYWLELKSFFESQHVTFPILLLRNSVLLATEKQVEKADKL
                     QLTWRDLFLNQQELFNQKTKLLSDFTIDFTAQKDALKKQFEALHEMAKTTDKSFLGAV
                     KAQEAKQIRGLENLEKRLLKAEKRVHQEQLNRIISLQNELFPNQSLQERKINFSEFYV
                     EFGAELIEILLKELKPLENEFKVVVL"
     gene            31536..31955
                     /locus_tag="P5335_01345"
     CDS             31536..31955
                     /locus_tag="P5335_01345"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRZ73553.1"
                     /translation="MTTNRTFTMIKPDAVENGHIGGILNMISEGGFKIVSLKLTQLTV
                     ADAQAFYAVHAARPFFGELVEFMTRGPIVAAILEKDNAVEDFRTLIGATNPADAAEGT
                     IRKKYATSIGENAVHGSDSDENAAIEGAFHFSGREQF"
     gene            complement(32026..33504)
                     /locus_tag="P5335_01350"
     CDS             complement(32026..33504)
                     /locus_tag="P5335_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRZ73554.1"
                     /translation="MIEEEYIRLIFGLKLRQIRTEKNLSLFGLAKVTGLSKSYLNEIE
                     KGKKYPKRDKILLLSEALDSTYDQMVSLKLDKNLAPIGEILQSRILKEIPLEIFGIKE
                     SNLIDIIAEAPVKVNAFISTLFEIAKHYNLTRESFFLAALRSYQEANNNYFDEIELQV
                     EKFAKAYSLDLTKKLNVADLEEILVDEYGYTINNEELTEHEELVYLRSVFVPKSKTLL
                     ISKSADISQRMFIYAKEIAYNYMEISDRLYTFSWIKFESFDQVLNNFFASYFAGALLI
                     PRKELVKELKLVFEQKTFNPTDFTSLISKFTDSPETFYQRLTNVLPKDFNLKNIFFLR
                     FNYKPERNDFQLTKELHITNLLEPHANENNEHYCRRWISIKTLSEIPSSKENHLFDTQ
                     ISSYNHTDNEYFVLSSATKDPFRNGYYRSIALGIMISTHSLSKIQFLEDESVKRKKVG
                     VTCETCSISDCFERVAPPKKIERKQRFEKIDKVVQSLMEKYS"
     gene            33692..35275
                     /gene="aceB"
                     /locus_tag="P5335_01355"
     CDS             33692..35275
                     /gene="aceB"
                     /locus_tag="P5335_01355"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HRZ73555.1"
                     /translation="METVEKTTVEVLNPTEHQSILTAEALTFLSLLHQKFNLKRIELL
                     KNRETQQEVFDQGFSPKFPKETESVRNSVWTAAPIPEKLLDRRVEITGPVDRKMVINA
                     LNSGAKIFMADFEDSCSPTFENLMEGQQNLIEAIRKTITLELNGKTYKLNKDTATLLV
                     RPRGLHLEDKNLICNEENLSGSLVDFGLYFFHNVVELQKQNLGIYLYLPKIEHYTEAR
                     WWNEVFEFAQNYFKIPIGTIKATVLIETITASFQLDEIIYELKNHIAGLNCGRWDYIF
                     SYIKKLKSHPDFMVPDRDQVTMTSPFMSAYSLRVIQVCHKRNILAIGGMAAQIPIKND
                     EEANKIAFEKVKADKEREVKNGHDGTWVAHPGLVSLAMKVFDENMPTKNQMHIKRGEL
                     NITEEQLLELPKGTITENGVRKNINVGILYLESWLNGTGAAALYNLMEDAATAEISKA
                     QLWLWLKKEVKLDNGILVSKTLYEKIIQEELVKIEEYVGAERFKSGQFELATRLFSNM
                     INSEKLDDFLTLEAYNYIK"
     gene            35352..36629
                     /gene="aceA"
                     /locus_tag="P5335_01360"
     CDS             35352..36629
                     /gene="aceA"
                     /locus_tag="P5335_01360"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HRZ73556.1"
                     /translation="MKTEDRIQALITDWTTNPRWKGIERPYTAEKVVTLQGSYQIEHS
                     IARRGSEILWKKLNNQDWVAGLGALTGNQAIQEVEAGLEAIYLSGWQVAADANIAGEM
                     YPDQSLYPANSVPIVVKKINNALLRADQIQTVNKIENKKDYLVPIVADAEAGFGGNLN
                     AFELMKSMIEAGASGVHFEDQLSSAKKCGHLGGKVLVPTQEAINKLIAARLAADVMNV
                     PSLIVARTDADAANLITSDVDKRDRKFITGERTPEGFFFVNCGVEQGIDRGLSYAPYA
                     DLIWMETSNPDLEYARKFAEGIHAKFPGKMLAYNCSPSFNWAAKLSVAEMETFREELA
                     EMGYKFQFITLAGFHALNTSMFELALAYKKRGMAGYSELQEREFALQKEGFKAVKHQN
                     FVGTSYFDEIQNTVTAGLSSTIAMKDSTEKAQF"
BASE COUNT        11821 a         6301 c         6493 g        11855 t
ORIGIN      
        1 ttttttcttt ccccgacccg ccgaggcggg tggaaaaaaa cggtgcttcg aaatgaataa
       61 atgcatgaac cctcgaaggg tttggaaccc ttcgagggta ggatgcttcg acaggctcag
      121 caggacagtt ggtttttgaa ttgagttttg attgattgtt tgtttttctt gtttgtttta
      181 tcactcgaac taaaatttta tgcttatctt tccctatacg tttacttaat aatcaagggg
      241 tcaccaaaaa gattccttcc tttctttata tcactatgga tgaggctatg aaatacaacc
      301 gctaccttaa aaacaccccg aacgtttctg caatagggct gggtgcctgg caactgggca
      361 gtaattccgg atggcagggc atgactgaaa aagaagctct ggaattagtt gcgaaagcct
      421 tggggtatgg tataaatttt tttgatacgg cacccaatta tggccacggt tcgggcgaaa
      481 ctcgcctggg taaagccttg aagggcgtgg acagagaccg tattgtcatc aacactaaat
      541 ttgggcatac tgattcgggc acgaccaatt atgcgtcgaa ttatatccga gaatcgttgg
      601 aaggcagcct gagacgcctg caagtggatt atgtagattc gcttcttttt cataacccac
      661 cccttgccta cttggacggg actaaaaatg accattacga cgtgctggaa cggttagtgg
      721 aagaagggaa aatcaaagcc tatggtgcat cgttagaccg ttttgaagag atgcagttgt
      781 tgatgaacac gaccaacgcc caggtgattg aggctttttt caacatcctg catcaggatg
      841 ctgctcgggc ttttgagttg gctaaagaaa aagaggtgcg aatcattgct aaaattccac
      901 ttgattccgg ttggttgtcg gggaaatata cggccgaaag cacgtttgat gacatccgaa
      961 gccgctggtc gagagcggat attgccaccc gagcccattt ggtgacacgc gtgaaggagc
     1021 ttgttggggc ggaagaaaac ttggcgtctg tggcgttgtc gttttgtttg ggttatgatg
     1081 cgatttcgac ggtgattccc ggaaatgtgt ccctcgcaca actggccagc aatgttgaac
     1141 ctgtgcatcg tccgctttct aaggaactta ttgcccaatt agaagcgttt tatttgaacg
     1201 aagtggagcc gttacgattg ccttggtaag gttgtgcttc agctgcggga gaattattat
     1261 tcagcaagtt cctttttctt ctctttcgtt tacacttgtt tagattggga cgataatttt
     1321 aaagtagcgg tgtggcgtgc cggatttaaa ttcttatttt tgtgaaaaat taatttaaag
     1381 ccttcacttc catacacatg aaaaaaatac tactttcctt tttcatcgtc ttccctgcct
     1441 ttgtttttgc acaaataggg tttgagcaac atgtggttat tgataacacg tttggcacac
     1501 cctatccgca acgcgttgca gcgggagata ttgatggtga tacctattcc gatgtccttg
     1561 tgggcggttc agggaaacta gcctggcata aaaacacgaa tggtttaggt acgttcagca
     1621 cggctaaaac agtggctccg ggcctttctt ctttcaactc ggttgcgatt gcagacatgg
     1681 ataacgacac gtatcctgac gtggtgtaca gcatttggag cgggaatctg gcgaaagtgt
     1741 attggcaaaa aaacacggat ggactgggta cgtttggtgc cgctattcaa attaacacca
     1801 acaacaatta cctcatgaaa ttggaagtaa ttgactttga taatgatggc gacaacgatg
     1861 tggtgcttaa ttcttttgac aatgtaattc tcttgaaaaa tgatggtgcc ggtgttttta
     1921 cgacgtctaa ccttttggga gccaataaca gttttcatat tgtggatgtt accggagatg
     1981 gcctcgcgga tgtgattaga gttgcgggtt acgacttggt tgcctttaaa caaaatgtgg
     2041 acggaaccct tacgctgtta gataacatgg acggctttgc tcaaaccacc cttattttca
     2101 gtgctgatat tgacaacgac ggcgatacag atattttgac aatctttgaa aacggtggtg
     2161 ctcaaagacg catcaaatgg tatcaaaaca ccaatggttt aggcactttt gccgccaacc
     2221 aagtgttagt cactttacct aatttagcag tgacttcctc caacgacacg aaaggattgc
     2281 agttttctga ttttgatggt gatggaaaac ttgatattat gttgtcagaa tcctcaacga
     2341 acaaactttg ttggtataaa aacctaaccg gtactacttt tgggacagaa caaattatta
     2401 caaccgatgc ctcgaatgtt agagaggccg ttgctgttga tgtaaacagt gacggtgatg
     2461 acgatatcgt ttccgtttcg tatgacgatg gcgatgtggc ttggtacaaa aacacgaatg
     2521 gtttaggggc tttcagtgct gccaacatgg tctcttatta tgcatattct gtgaatcata
     2581 ttgatgtagg agattttaat aacgatggaa ctccggatat actttcgact tcgcatggtg
     2641 ataaaaaagt agcgtggtat aaaaacgtgg atggtttagg atctttttcg caaccgcaag
     2701 ctttgatttc taaatccatc aacggcaccc gaaacggtta tgcgaaagac atggatggtg
     2761 atggtgattt agatgcttta tatacgtatt acttagatga taacgtcgat gtatttaaca
     2821 ttcgatggaa agaaaataat ggttccggga tttttacaac ggaacatgtg gtctatgtgg
     2881 gtatccccga tttgtatgct atcacgcctt gtgatattga tgcggatggt gatacagata
     2941 ttgttgctgt ttttaacctc aacaatttga tggttttgaa aaacaatggg aacggcacgt
     3001 ttgccccaca acaattgttc aactttccgg tggggagttt gggttatata gtggtggaag
     3061 atgtggatgg cgatggcgat ttagatgtta tcagttccgg ttccaacaaa tttgcctggt
     3121 atgaaaacac aaacggtcaa ggcgatttga gtgtagaaca tttgattcct ttaagtaata
     3181 atagttcgcg ggttttgaat accgcagatt tagatggtga tggtgatcaa gatattctgt
     3241 tcatcaatcg aacaccgaat aaaattggtt ggtttgaaaa cacagatgga gcgggtactt
     3301 ttggtcccga aattattttg gctacagtga ctaaaccgtt atccattctg gcggttgatt
     3361 tagatggaga cggcgatcag gatattgttt gtgatgcgga acaaggcttc cgtttacaat
     3421 tgtttgaaaa caatggaaat gctactttta gtgccggttt tgaaatcacc agccagattg
     3481 gtcgcgtgac tagtcttgtt gcagaagata tggatgcaga tggcgattta gaccttatta
     3541 catcctctta tgatgatgat caagtggctt ggtttgaaaa cctaggcagt tttagaaata
     3601 cgttgcaagg aacagttcgt atcgacaccg atagtaacgg ttgtaattcc acggatgttg
     3661 ttgtgccgaa cgtattggtt accaccgacg atggagcaaa cactttttct acttttaccg
     3721 gtgcttccgg acaatacgtg ttgtatgcca atgaaggtaa ttatacgacc agcgtgacca
     3781 ccccgttacc gaattatcct ccaaatccaa acagtcaagt ttggtccttt gaaggggtaa
     3841 accaaacgca agtggttgat ttttgtttag aaccttctac cctctttgaa gatttagaag
     3901 tgagtttggt tccgttaaat ttggccagac ccggatttgt ttcgaaatac cgtattcata
     3961 ttaagaatac cggcaccaac cctatgaatg gtgttttaac gtttacgtac aaccccgtga
     4021 agtttagttt tatcacatcc agtacagcta ccagttctca aacagcaaac tctttgagtt
     4081 ttacctttac gaatttaaat gcttttcaaa ccaagattat tgatttggaa tttcaagtgg
     4141 ccacgatacc tactacgaca ataggtgaat cggtactttt tactgcgact ttaaatgaaa
     4201 acacgaatga cgtaacccct gaaaacaata cttttttgtt tcatcaactc cttgtgggtg
     4261 catatgatcc caatgatatt caagtcgtag aaggaccgga aatcgctttt gaagacgtgg
     4321 ataactattt acattacatt attcgttttc aaaatacagg taatttttat gcccaaaagg
     4381 tcgtggttac gaacgaactg gatgataaat tagactggac gacttttcaa ttagaaagtt
     4441 acagccatcc taatcgtgtg gaacttttga atggaacctc agttgatttt atttttaacg
     4501 ccatttattt acctagcgtg aaccaagacg aagaaggttc gcaaggttat attgcttata
     4561 aaattaaacc caaaaccagt gttggtttgg gtgatgttat cagtaatacg gcttctattt
     4621 attttgatta taatccggcg attattacga atactgttac tacaaccgtg gtcgaaacag
     4681 ttttgtccag tccttcattt gaaacggaat cggtgactct ttatccaaat cctacttcag
     4741 gttggttaac cataaattcc aaagcgatta ttagtaaaat cgaagtgtat aatcaattag
     4801 ggcaacaggt actccaaagc gatacttcct cctcaataga cctttctgcg ttgagccaag
     4861 gggtttattt tattcgtttc gttgattcga atgagcaggt attgattaag aaagtgatta
     4921 aacaataact atttttagtc tttgtagttt taagagggag ttggcctaac taaccgaaaa
     4981 atgtaaaata atgctacact agtagtaaag tttgcattat tatgttttat tttaaagtag
     5041 aaacgtagtt atttaaaccg ttgttaacca tgcctaataa aagacttaaa attattctat
     5101 ttataatagc cttgctctta cttatccctt ttgtggcaat gcagtttaca gaggaggtga
     5161 attggcaggc aggcgatttt gttgttgccg gtgttttgtt gctgggaact ggcttactct
     5221 gtgagttagt cttacggaat gttactaaaa cgagctatcg tatagttctg tgtatgacca
     5281 ttttgatttt gttatttctt atttgggcag aactggcagt gggtcttttc gggacgattt
     5341 tcagtggatc atagtgtcaa aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnngcctttt cgtcaatgaa tccggggaaa aaagctaaga aaaacaaggt tacttttggg
     5521 tttaatacat tcatgatgaa cccttgtttc actaaagcca gtattgattt ttgtggcttt
     5581 gccgaggtat tcaaagtaat ggaagcagga gctttataga cttggtaggc aatccaaagt
     5641 aaataacaag ctccaaaaat tttgattact gtgaaaatta tttccgattc tttaatcact
     5701 gctgaaactc ctaaagcgat taaggtggtg tgtaccaaaa ttccggagac taaacctaat
     5761 gcagtagcaa tgccatactt tttcccattg gagatgcttt gggtgaggac atacagaata
     5821 tccggtcccg gggaaagcgt taaagcgatg gaagcgagga gaaacattgg aattacgaat
     5881 taggaattat acttctttca aaattcggtt gttgatttcc ttgattaatc ccggaccttc
     5941 atagataaag cccgtataga gctggatgag actggctccg gcattcaatt tttcgatggc
     6001 ttcatcagca gaatgaattc ctcctacgcc aatgattgga aatgctttgt tgcttttttc
     6061 acttagaaaa cgaataactt cggtggaacg tttggttaag ggtttaccgc ttaatccgcc
     6121 catttcagtt ttattttctg aatgtaatcc ttctctagaa agcgtagtgt ttgttgcaat
     6181 cactcctgct atttgagtct ctttgacgat gtcgataatg tcgagtaact gctcgttcat
     6241 taaatcgggt gcaatcttga ggaggattgg tttttgtttc ggtttcgcga ggtttcggtt
     6301 ttgtaaggtt tgcaacaatt gcgtcaacgg ttctttgtct tgtaacgccc ttaagttggg
     6361 tgtgttgggt gaactcacat tgaccacaaa ataatctaca tgtggaaata aggcttcaaa
     6421 acaactctca tagtcggctg ttgctgtttc gttgggtgtg tttttatttt tgccaatgtt
     6481 tccgcctatt aatacaccgt tgttcttttt taatcgttct actgcttcta aaacaccacc
     6541 attgttaaac cccattcggt tgataatagc ggcatcttct tttaatctaa acaatctttt
     6601 tttgggattg ccctcttgtg gtttgggcgt taaggttcca atttcgatga agccaaatcc
     6661 gaagttggag agttctttgt atagttttgc atccttgtca aatccggcgg ctaatccgac
     6721 agggtttttg aatttcaagc cgaatacttc gcgttccagt tttggatttt tcacttcata
     6781 catggcttgg aagagttttc caacgcccgg tattttatgg agaaaacgaa tgagtgaaaa
     6841 agtaaaataa tgcacttgtt ccgggtcaaa gcagaacaat atcggtcgaa tgagtagttt
     6901 atacattatg gttgtgttgg taggcaaaaa taatcattct cacttacatc gctgctaaac
     6961 acgaaacaaa ataatcgatt tcttctttgg tattgtgttg attaaatgac aggcgtaagc
     7021 ttggtttttt taattctgat tccgagagga attccaccaa cacatgggat ggttttatac
     7081 tacccgactg gcaggcactt ccacgagaaa cggcaattcc tttcatatcc aattcaaata
     7141 aaattatact tgttttatcc tctgccaaag gcaattgcac atttaaaatc gtgtaaaatg
     7201 tttccagtcc gccattaaat tttacggaag gaaattgagc cgtaagtttt tcaatggtat
     7261 atttttttaa ttcaaagatg gtatctcgtt gactttccaa gccttcataa gcgaattcca
     7321 aggcttttgc tatgccggca atttggtgta ctgcttccgt acccgcacga agcccttttt
     7381 cctgctcccc tcccactaac aaggctgcta tgggcgtgtt cttttttata aaagcaaatc
     7441 cgactccttt aggtccgtga aacttgtggc cgctagccac caggaaatca atgttaagtt
     7501 gttgtaaatc aagctcggtt tttcccattg attggacagt atccgaatga aaataggctt
     7561 tgtgcagttt gcataactct cctacccgct caatatctaa gaccacgcca gtttcattgt
     7621 tcacatgcat caagctcact agcgttggag tgtggctatc caacaaagct gtcaattgca
     7681 aataatcaac ttgtccattc ggtaaaacct ttaagaactc tacttcgatt ccgaattcct
     7741 tttgcaatgc caggatgggg tatactacag catggtgttc cattttggag gtgataatgc
     7801 gttttatccc aatcttttta acggcatttt gcaaaatcca attgttggct tctgttccgg
     7861 aagaggtgaa aattatttcc tgagccgtac agttgagttg ttttgcgatg ctttttctgg
     7921 cggattccaa gactgctttt gctccccgtc cgaaactgtg tgaggaagaa ggattaccat
     7981 attccgattg catcacgcga gtcatttcct gaattacttc gggatgaatg gcggtggtgg
     8041 aggcattatc gaggtatatt ttgttcattg aaagaatatc attgtaatta aaaagtattt
     8101 atttataaga ttctcttcct taattttttc cctgcttcgc aggagattcc tatatcgact
     8161 tcgtcatctt tcggaatgac aaaaaaaatt aatttagagg aatgaaacta atcgctgttc
     8221 caccaccggc ggcaaggatt aatttcagtt ttgtttttga tgttacttcc attgttttaa
     8281 ttgtgtacgc ttttggattt ttttcccaat gggcgtcttt tccatcttga taaatcgtcg
     8341 ctttatattt tttgccgggg gttaaaaagt ctaaagttat ttccgaattt ctggcatttt
     8401 catctgttat ggctcctaaa aaccaagatt cttttccttt tgtttttcgg gcaatggtga
     8461 ggtagtcacc aggttcagct tcgatgattt tagtttcatc ccaatctact tccacatctt
     8521 tgatgaattg aaatgcatca ggataacgtt cgtagttttc cggtaaatct gctgccattt
     8581 gcaatgggct gtaaatcgtt acatacaaag ctaattgttt tgccaacgta gtgtgcacct
     8641 gttccttttt agtagcgtcg tagtggctca ttttaatttc aaaaattcca ggcgtgtaat
     8701 ccatcggtcc acctaaaaat cgtgtaaacg gaagaatagt ttcgtgcata ggcggattac
     8761 cgttgctcca agcattgaat tcatttcctc gggctgcctc cgccgcaatg tagttagggt
     8821 acgttcgatg aacaccggta ggccgagatg attcgtgcga attgaccatg attttatatt
     8881 cagctgctct ttccactacc caattgaaat ggttgaccat ggattgacca tcgtgccatt
     8941 ctcctctggg gataatttta ccaacataac cgctttttac tgcgttataa ttatgttttt
     9001 tcataaaaga aaaagcacgg tccaaacgtc tttcataatt ggcaaccgaa ccggatgttt
     9061 cgtggtgcat gatcatttta attcctttag attcggcata tttttgcact tcctctacgt
     9121 tgaaatcagg ataagcagta acaaaatcaa acacattttc tttccaattt ccgaaccaat
     9181 cttcccatcc gatgttccac ccttctacta atacgccatc aaaaccatgt ttggcagcaa
     9241 aatctatgta tcgttttgta ttttcagtag ttgctccatg ctttccggta ggttttaact
     9301 ccgcatcctg agccatttgg gcgttttgtg aaccggcata gtcccaagta gctttaccta
     9361 catgcatttc ccaccaaatc cccacatatt tcattggttt aatccaagaa gtatcttcta
     9421 tttttgaagg ttcatttaag ttcaaggtca tttttgaagc gagaattttt cgggcatcgt
     9481 cactcaccat gattgttcgc caaggtgaat ttgccggagc ttgtaaatac gctttatcac
     9541 caatggcatt gggcaccaaa tgagattgca tcaaaaaggt agttgtatct aattctaaat
     9601 gcatgatggg ataattgacc accgcagctt caaagatatt gatatacaat ccatcggctg
     9661 atttcatcat cacaggagat tgaatacggg aatttgccat tgggcctttc ataccaatgc
     9721 cattgttcat gtctaattta gacacatcca cttgggacaa ttttgtttca ttgtacacat
     9781 attcttggct atcgtagtca ccgggaatcc aaaaagcttt atggtcgcct actaaggcaa
     9841 attgggtgac ttcatctgaa atgacaaaat aattcaagtt ttcttgtgct ggaaattcat
     9901 atcgaaacgc taacccttcg ttgaatagtc tgaacacaat attcattttc cgtttggagg
     9961 acttttgaaa taattcaagc accaattggt tgtagctatt ttgaatttcc ttttgttcgc
    10021 ccaaaacagg tttccaattt tcatttactg cagaaacagt tgagtttaca atttcaaatt
    10081 cggccgccaa tgtactcgta tttttaagta ccacccctaa aaaaccagtt tgaataaccg
    10141 gttttgtttt ataatttaaa ctataaaaag gacgtccaat tttgtttact ccaacggaca
    10201 attctacttg tttatcaggt gattgtagct tttgaccaaa agtaatcatt gataataaaa
    10261 aaatcgaaat caagaaacta tttttcattt tatatctgtt tttaataagc gtttcaaaga
    10321 taagcataat ggttaaattc atactaatta aaaagaaatg cttttgaaag aaattaaaaa
    10381 tcctattttt gctttaaatt attttgatta tgaaaagatt tttagggttt ttggtacttt
    10441 tgctaacact gaacgcttgt gatgacggag attttgatat agaaaccttt aattttacag
    10501 atgtaaacac aaaccaatgc aatagtggag ccaacggttt ttttatctat aaaatcaaca
    10561 atactgaagt tttgcttgtt caaattccgg agagtaattt tatcaatgaa attaccgttc
    10621 cgggcactcc gagaacagtt aatattggag gggccaacaa agtaatttat agaatttatg
    10681 acggaaccgt aaccacaggc gacatttgta acactatacc tccagccaca cctgtagtta
    10741 ccgaagagtg gaatgctctt gccggaacaa ttgaaatagt gaccactgta aacaaaactg
    10801 ttgatgaaac gcttaattcc tctatcatta cgggttatac acacaatatt attttacgca
    10861 atgtcaattt tcagaaagga aatggaaaag agcaattatt tgaatcgttg acgtttggaa
    10921 actatatcac ctcagccacg gcgatggcca attttgatgg ttttgcaata aaaaactgtg
    10981 cgacagatta taatttttta tataaaatat ccggtagtca agctttagct tttgctgtag
    11041 atgccacgtt atttcaaaat gaagttacgc ccttagatag tccaagaacc gctttaattg
    11101 acgatatcaa tcaacttgag ttacggtatt ataatgataa tattaatgat gcttttgctt
    11161 gtaccacacc accgctttct tatccggtat tagagcaaaa atgggtatct gaagacggca
    11221 ttaccggtgt aagcggaatg attgaagtga ccactacgga agaatatttg attcctactg
    11281 acaatcagag tccgttggtt ggctatcgtc aaaaagtaac cctaaaaaaa gtgaccatgg
    11341 ttagaaacgg ggtaagcttt aaattgggag atgtatatga atacggcgaa tttgtaacgc
    11401 ctttataaca tttttttctg tataaaataa acctccgttg ctcctaaacc atatttttgg
    11461 taatccgcat cttgaaactt gatgttttcg tatcggccta aaagaaaatc taattctgct
    11521 tttaaaacac cttcacctac gccgtgaata aaaacaactt tcggcatttt atttttgatt
    11581 gcaaattcca attgccgctt tgcggtttcc atttgtagat tgagaatatc gtagttgttc
    11641 attccccgtt tagaagggac caatttttca atgtgcaaat cgacttctaa tacaaaatca
    11701 tccttacggg atttcttttc tttgacaaaa ctacgcttag ttggttccgt tttttcctga
    11761 attgctttgt ataatttatc tgttgaaata aaactactta attcactggt aatgtttatc
    11821 ttaattagtt cattgacaaa atatgtcatc agaaaaccgt ctgtagtttc tatctccacc
    11881 tcgttgtttt tgaaggaaac aaccacacca gaaatgtcct cgtctaacgc tgcaactttg
    11941 tctcctatat taaacatcct cttcttcttt ttcttgattt ttacttggta ctttttgcat
    12001 aagtttcatt acccctatca ttaaaattac aattgcagca acttttatat agaccttata
    12061 attagtttct ttcggtgtat aaaaagcaaa aaaaactgcg attaacatga ttggaataat
    12121 aacttttttc atggtgaaaa tttaaattga aatctcattt cattggattc caaatgtaga
    12181 aattaaaatt ataagtttac tacttgtaaa gcaaactctt tttttgtaaa attgtatgat
    12241 taatataatt tgaccttatg gaagaataca tgcttccctg tttatcaaaa aaactatttg
    12301 gcatagattg tttaggttgt ggtattcaac gagccgttgt tttattattc aaaggcgaat
    12361 ttgccgctgc tttttacatg tacccggcca tttatactct gcttttattt tttgctttta
    12421 ttggcatccg ctttatcgat aaatccagaa actaccacaa aataattatg ctttttggat
    12481 ccgttacagc cgtcatcatg gttgtttcct atttgtataa agtcatagtc tattaaataa
    12541 atctaagcta taaaaaaagc cactttaaaa gtggctatct tcttattttt caaactcttt
    12601 cagtgttttg ataataattg aaacacaatc caacaattgt tcttcattca taaccaaagg
    12661 tggagcaaaa cgaataatgt ttccgtgggt aggtttcgca agtagcccgt tttcagctaa
    12721 tttgatacaa atatcccatg ctgttgagct atcttctgta tcattaatta caatagcatt
    12781 cagtaaccct ttgcctcgca ctaaacttac gatggaacta ttttcaatat actcgtttaa
    12841 ttttgctcta aataatttac ctaaagcacg agcattttga attaaatttt cttcttccac
    12901 tacttctaaa gctgcttttg ctactgcggc tgctaatggg tttccaccaa atgtagaacc
    12961 gtgttgacca ggttttatga catgcatgat attatcatct gctaaaactg ccgaaaccgg
    13021 gtaaacgcca ccggaaactg cttttcctaa aatcaggata tcgggtttag catatgtatc
    13081 ttgtctttca caattatttt cgcaagtaca attgccacaa acagccaata aagaacctgt
    13141 tctggcaatt ccggtttgca cctcatcggc aataaaaaga acattgtatt gatcacaaag
    13201 ttctttcact tgttctaaat agttttctgc cggaacataa acaccggctt ctccttgaat
    13261 aggttcaact aaaaatccgg caatattttt attgttttct aaaacaaatt gaaaggcttc
    13321 aatatcatca taaggaattt tgataaatcc tccggtataa ggaccaaagt tttttctggc
    13381 attttcatca ttagaaaaag agatgattgt agttgttcgt ccgtgaaaat tattgtcaca
    13441 aacaacaatt atcgcttcat gttcatcgat accttttttt tcataagccc attttctaca
    13501 aattttgata gctgtttcaa ccgcttcagc tccggtattc atgggtaata ttttatcaaa
    13561 cccaaataat tcggtaatgt atttttcgta ttttcctaat ttatcattgt aaaaggccct
    13621 tgaagtcaac gttaacgttt gagcttgttg tataagagca ttcactattt ttggatgaca
    13681 atgaccttgg ttcacagcag aataagcgga taaaaaatca taatattttt ttccatctac
    13741 atcccacaca tatactcctt ctcctctact cagtacgacc ggcaacggat ggtaattatg
    13801 agcaccaaat cgatcttcta aggcaataaa ttcttttgaa ttgatttttt ctaaaactga
    13861 cattttctta aatttaaaat gaactacttg tcaattccta cttttggaga gaaatcatcc
    13921 taaacaacag caaaaataac gaatattcta ttgaaaataa tgagctgatt cataaaaaag
    13981 actaaactat aaaactcttt attaaccaaa cgttaaaaaa agattattat ttaagaataa
    14041 atctttattt ttgtaaaaat taaaataatt gacatgaaaa agattctatt tttattatta
    14101 agtttcactt tagtcgggat ttcctcttgt tcatcagatg atgacaaatc agacgtgaat
    14161 tatgttactg caaaattcaa caacgtcgaa ataaaattta atatcatttc cgttgataaa
    14221 attgattatg acggatattc agatattatt gtaaccgcta caacaaacag tgaccctact
    14281 aaagtaatta ctattgcttc tgagtatggt gttaccggac aaaatgaaat ttggggattt
    14341 gattatgaaa cagcagatga atatttcgtt caggatgctg caaatttcac ctcaaccctc
    14401 acaacaaatt ctgatggaaa atacatcggt acgttttcag gttctttagt ggaagatacg
    14461 aatggtaatg tgatggtttt gacaaacgga gcctttaata tcgcttatta aaaaataaat
    14521 tataagttct aaaaacgcct tctttcgagg gcgttttttg ttttaaaaaa taatttcatt
    14581 acccgattaa ttttcttatt gaatacctta tttttgccat atgggaagaa aaaacacaga
    14641 caaaataatt tttaaagaag taaccgtttt agatgccgga gcaaaaggcg tttctgtagc
    14701 aaaagcagaa gatgggaggg taatttttat atccaatgtt gttcctggcg acattgttga
    14761 cgttcaaacc ttcaagaaaa gaaaatccta ttttgaaggt aaagcaattt attttcataa
    14821 actttcaccg catcgcgtaa aaccggagtg tgagcatttt ggagtttgtg gcggttgcaa
    14881 atggcaaaac atgaattata atcaacaatt gttttataaa aacaatgaag tttataataa
    14941 tttaaaacga attggcaaga ttgaacttcc tgattttgaa cccatattag gctcagcaaa
    15001 acagtttttt tatcgcaata aaatggaatt ttccttttca gacagtcgtt ggctcactga
    15061 aaacgagatt caacaagagg acacgattga aaaccgaaat gcattaggtt ttcacattcc
    15121 aaaaatgtgg gacaaaattt tagacatcaa caaatgtcat ttgcaacaag atccttcaaa
    15181 tgctattcga aatgctatta aatcgtttgc tacagaaaac aatcttccat tttttaatac
    15241 ccgacaccaa gaaggacttt tgcgaacctt gatgattcgg acaacgtcta ttggagaaat
    15301 tatggtttta attcaatttt ataaagaaga taaaacgcaa cgcgaattgg ttttaaattt
    15361 tttaaaagat aaattttcgg aaatcacatc tttactctat gtaattaatg gaaaagcaaa
    15421 cgacacattg tatgatcaag atattaaact ttttagtggt cgagattata ttttagaaga
    15481 aatggaagga ttacaatttt caatcaatgc aaaatccttc taccaaacga attcagacca
    15541 agcttatgaa ctgtataaaa ttacgcggga atttgccggt ttaaccggaa atgaattggt
    15601 ctatgattta tataccggaa ccggaaccat tgctcaattt gtttcaaaaa aagctaagaa
    15661 agtaattggt gtggaatctg ttcctgaagc tattgccgat gccaaagaaa atgctaaacg
    15721 caataacatt actaattgtg aattttatgt tggagatatg aaagaagtct tcaatgcaga
    15781 ttttattgct aaacacggaa aacctgatgt tatcataaca gatccgccac gcgacggaat
    15841 gcatgcaact gtagttgaac aaattatgca aattgcccct gaaaaagtag tttatgtcag
    15901 ttgcaattcc gccacacaag caagagattt agctttaatg gactcgcttt ataaagttac
    15961 ccgtgtacgt ccggtagata tgtttccgca gacgcatcat gtagaaaatg ttgtactttt
    16021 agaaaaaaaa taaatgaaac gatttttgtt ttttgtggtg ttattatttc tttttcaagg
    16081 atgtgaaaaa gacgacatct gcacacccga aactcctact acacctcgac ttatcattga
    16141 gttttatgac agcgtaaata catcggtaaa aaaggtagtg gccaatttaa aagcagaagg
    16201 ggaaggagcc gatacggcac ttacatttga tttagtaagc acaatagaac ttccattaaa
    16261 aacaaatgaa gattttacac aatacaaatt aacaatcaat tcaacttctg caacggaagc
    16321 gaatatggac agtatccgta tcaattacac ccgcaatgat atatatattt caagagcctg
    16381 cggatttaag actaattttg atttgaattt attcaatccg attgaaaatt caaatcccgt
    16441 tggtgatact gaattttgga tagatactat agaaataatt aagaacaaaa ttgaaaccga
    16501 agatgatgtc catatcaaaa tgtattttta gtatcggttt aattttggtt tccatttcta
    16561 ttggagctca agaaaccaaa aaagacagta ttcctttgaa aactgagcgt tacggtgttc
    16621 gtgttggaat tgacttattt aaatttggta aaagtttttt tgacgaaaac tataaaggac
    16681 ttgaacttgt gggtgattat agactcacta aaaactatta tctcgccggt gaattaggca
    16741 atgaaaacaa gacaactcaa gacgatcgat taaattttac tacgaaaggt tcctatttta
    16801 aagttggttt tgattataat gtgtatgaaa actggcttga tatggaaaac ctaatataca
    16861 ttggtttgcg ggtgggagcc agcacacatt ctcaggaaat aaacacctat aaagtatata
    16921 atccaaaccc ttattttggg gaagctcctg aagtaatttc cggagaaaaa tttgatggcc
    16981 tttcagccca atggggagaa tttctgattg gagtaaaagc cgaattgttt tccaatttat
    17041 atgccggttt tagttttaga ttaaactatt tgataaacac caaagaaccg gatggttttg
    17101 aaaatcttta tattccggga tttaacagaa cttatgacgg cacttttgga gttgggttta
    17161 attatactat ttcctatttt attccaattt ataagaaaaa aatagagcct aaaaaggaag
    17221 ttaaaaaata aattataatt cttttatttc tttactaaaa atccacttca taaaaatttt
    17281 agagccttct tttgtagtaa ttacttgaaa tttaggagct aaaatcaacg taattacaaa
    17341 agctaaaaaa ggaatccaaa taccttttaa aaaagaaaaa taggaaattg caaaacgcaa
    17401 cggaataaaa aaagcggcaa aagacagtaa ctggtaaata aaaactttgg tttttatatt
    17461 cattagattt gaatttttac caaaagtaca aaaaaatcaa gttttaatat tcatatttct
    17521 attgaagaac gcaacctata aaagaattat tttatattta ccctttcatt atacaaacca
    17581 attctccaaa accaattcaa cctgatgctt tacaaaagtt tgaatctcaa agctccgaaa
    17641 gtaacctttg aacaagccgt tactcaggga ctcgctccgg acaaaggact gtattttcct
    17701 gacacaatta tcccgctaga acattctttt tttgaaacaa tagcaactcg ttcaaatgaa
    17761 gaaattgctc atactgttat taaacaattt ataggaagtg aaattgaaga aaatgattta
    17821 agaaaaatca tttcagagac attgtgtttc gattttccat taacaaagat tgaagaaaac
    17881 atttattcct tagaacttta tcatggtcca accatggctt ttaaagatgt aggagcccga
    17941 tttatggcaa ggtgtttagc ttatttcaat aaaaacaaac ctaaccagaa gaacacaatt
    18001 ttagtggcca cttccggaga taccggtgga gcggttgcta acggtttttt gaatgttcaa
    18061 ggcgtagaag ttgttattct ctacccgagt ggaaaagtta gtgatattca ggaacgtcaa
    18121 ctcaccacgt taggtcaaaa tatcacagcg ttggaagttg atagcgtttt tgacgattgt
    18181 caggatatgg ttaaaagagc atttttggat cctgaattag ctcataaaaa tctaacttcg
    18241 gccaattcaa ttaatgtagc ccgatggctt ccccagctat tttatttttt ctttgcttat
    18301 aaagaattaa aaaaaatgaa tttgcctatt atcatttcgg ttcccagtgg gaatttcgga
    18361 aatatttgtg ccggaatcct tgcaaaaaaa ttaggtttac ccattcatca ttttgtagca
    18421 gcaaccaata tcaatgacac cattcctcgt tttatgttga atggcatgta taacccaaaa
    18481 ccgtcaaaag ccacgttatc aaatgcaatg gatgttggta atccaagtaa ttttatacga
    18541 attcaggaat tataccagca tgatttttcg tcttttaaaa atgatttttc gtcctatact
    18601 ttttctgatg aagaaactct tgttgcatta aaggatttgt atacaaaaaa tcaatacata
    18661 gcggaacctc atggagctat tggctattta gggttaaaag aagaaatgaa aaaacacgga
    18721 aagtccgttg gagtattttt agaaaccgct catccgataa aatttttaga tactgttgag
    18781 cctgctttag aaatctgttt acccattcca actcagattg aaagcgtaat gaacaaagaa
    18841 aagaaaagca tcaaaataaa aacatatact gatttaaaaa aatatttaag ttaatacaaa
    18901 atcataacta tcaaaaaata ccacacttta ttatgattca aactcaaata caaaaactac
    18961 ttcatttttc cacagttaaa aagtcaataa tttttttata tttgaataat gaaaagtaaa
    19021 tcgaacatca tcacctttat ttttattatt acagctcttt ttttagccct tataagtcat
    19081 ttagtttttc gtaataattt aaaaaataat gttgaaaatt tcaattccta tcatttcatt
    19141 gttgatgctt gccttattat aggtgccggt ttattaattc gttttgttat tgcccgaaat
    19201 gacagaagaa acattggtat actaaaacaa cttcaaacca ttaacacgga aatcaaggat
    19261 tccaacgaac gctatgatat tgtagctaaa gcaaccagtg acaccatttg ggattggaag
    19321 attcaaaccg atgaatttgt ttggaataaa ggaattcaag gagtttttgg atacaaaaaa
    19381 gaagacgttg gaaatagttc caattggtgg tttgaccgga ttcatcctga agacagtttg
    19441 aaaatgtcag ttaaattata ttccttttta gaacaaaaaa cagaacgttg gcaagatgaa
    19501 tatcgattta aatgtgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag
    19621 aaaaacggtg atgaatattg ggtgaacttt tctatgatac cggttacaaa taaagaagga
    19681 gaacattcgc attggatttc cattcaaaga gatgtgacca aagaaaaaaa tcaggaaaga
    19741 gaacgcgaac aactcatcca ggaattaacc cgaaataata aagatttaaa acagttttcc
    19801 tatatcacct cacacaattt cagggctcct ctatccaatt taacaggatt attaaattta
    19861 ttagatgatg tgcctattga caatgtggaa ttgcaggaga tattaaacgg attcaaacgt
    19921 tcaactcatt tattaaatga aacgattgat gatttagtaa aggttgttat tattaaagac
    19981 aatccctcca ttcaaaaaga aattgtttcc atcaaagaga tttttgaaaa tgtttttttt
    20041 caattaaaca atcaaattaa tttatataaa ccaattataa atattgatat tgaagacaac
    20101 ctttccatca cgattaacaa agcttacctc gaaagtattg ttttaaattt aatgtcaaat
    20161 gcactaaaat accgttcgtt aactaaaact ccaaaaattt caattatagc tgtagaaaaa
    20221 gaaaattccg taaatttgat tattaaagat aacggaatcg gaattgattt gggcaaaaac
    20281 agtgataaaa tctttggact ataccaacgt tttcataatt atcctgacag taaaggatta
    20341 ggattatatt tagtaaaatc tcaaattgaa gcaatgggcg gttcaattga agtagaaagc
    20401 caagtaaata aaggaactca atttagttta acctttaaaa aagtataatg ttgaaatcta
    20461 tattatgtgt tgatgatgat ccaattactt taatgttgtg taaaaaagta attgttaaag
    20521 ctgaatttag tgaagaaata atctttgcca ataacggtca ggaggcttac gatttatttc
    20581 aatcttttgt tgtaaaaaaa gaccgggatg caatttttca atttcctcaa ttgatttttt
    20641 tagatttaaa tatgcccgtc atgaatggtt gggaattttt agatttattc aaagaaaact
    20701 tatcaaaaga attttctcaa actaaagtaa tcatactatc ctctaccatt gatccgtcag
    20761 attatcaaaa ggcaaaacaa tatgaaatgg tttcgcattt cctatcaaaa ccaattacag
    20821 ttgagatgtt acagaattta aaacctttat attcataaaa aaaatagctt tccaaatgga
    20881 aagctatttt tttatatatc taattgtact aattataact tagctacatg cttagttaat
    20941 ttagatttta aatttgaagc tttgttatca tgaatgatat ttttctttgc taatttatca
    21001 atcatagaaa tcactgatga taatttagct gatgcatcag acttatctgt agaaactctc
    21061 aatgctttga tagcattacg cgtagttttg tgttggtatt tgtttaatac tcttctcttt
    21121 tcgttacttc tgattctctt tagagctgac ttatgatttg ccattttctt ttaaataaat
    21181 gtttttatta aaattttgta gtccgtaggg gaatcgaacc cctgttacca ggatgaaaac
    21241 ctggcgtcct aacccctaga cgaacggacc attgttttcc tctagtttaa gattttcaac
    21301 ctaatattga acttgtagcc cgtgggggaa tcgaaccccc cttaccagga tgaaaacctg
    21361 gcgtcctaac cgatagacga acgggccaat tgtttattgc gagtgcaaaa atacaactat
    21421 ttttaatact cgcaatagtt acaatttatt tttttaattt tttttaatat gcttttgcaa
    21481 aaagcaccct tccaacagat ggttttccgc taaacatgca cattccaggc tcttccattc
    21541 tatttaatgg aatacatcgt attgttgctt ttgtcaactc tttgattttt tcttctgttt
    21601 ccggtgttcc atcccaatgt gcagcaaaga atccgccttt agaatctaat aaatttttaa
    21661 attcttcaaa agtatctact tccgttatat gagtgtttct atactcaaat gctctatcaa
    21721 acagatttgt ttgaattgtt tctaacaaat tttgaatata cacctcaatg ccctctttag
    21781 aaactatttc ttttgttaaa gtatctctac gagccacttc ataagttcca ttttctaaat
    21841 cttttggtcc tattgcaatt cggacaggaa cacctttcag ttcgtattca ttaaatttaa
    21901 acccaggctt ttgggtagtt ctattgtcaa atttaactga aatatttaat tttttaaggg
    21961 catccacaac attttgaact tccgtagaaa tagcttctaa ttgttcatct gacttgtaaa
    22021 taggtacaat aacaacttga attggagcta aatttggagg aagtactaaa ccattatcat
    22081 cggaatgagt catcactaat gctcccatca atcgagtaga aactccccaa gaagttcccc
    22141 aaacaaattc ttgagtccct tctgcattag caaatttaac atcgaaagcc tttgcaaaat
    22201 tttgtcctaa aaagtgagaa gttccggctt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22321 nnnnnnnnnn ntaacatcga aagcctttgc aaaattttgt cctaaaaagt gagaagttcc
    22381 ggcttgtagt gctttcccgt cttgcattaa agcttcaata caataagttt ccacagcacc
    22441 ggcaaatctt tctgattctg ttttaattcc ttttacaacc ggaattgcca taaaattttc
    22501 tgcaaaatca gcgtaaacat tcatcatttg aacagattca gcaattgctt ctgcttgagt
    22561 agcatgtgca gtatgtcctt cttgccacaa aaattctgca gttcttaaaa acaaacgcgt
    22621 tctcatttcc caacgaacca cattagccca ttgatttatt aataatggta agtccctgta
    22681 agattgtatc caatttttat aggtgctcca aataattgct tcactggtcg gacgaacaat
    22741 caattcttcc tccaatttcg cattggggtc aaccattaat ttaccttttc tatcggggtc
    22801 atttttcaat ctgtaatgtg tcacaatggc acattctttt gcaaatcctt ctgcattttt
    22861 ttcttctgct tcaaacatac ttttgggtac aaacagaggg aaataagcat tcgaatgtcc
    22921 tgtttctttg aattttttat ctaattcagc ctgcattttt tcccaaatag catatccgta
    22981 tggttttatc accatacagc ctctaacagc ggaattttca gctaaatcag cttttaccac
    23041 aagttcatta taccactttg aataatcttc ggctcttttg gttaaatttt tactcatttt
    23101 gaattatttg gcataaaaat tgttttataa atattaacta taaagttcag caaaactaac
    23161 tatttttgta ttaccaacaa taaaaatgct ttgataatga aaacaaaata tctcacttcg
    23221 tttaaaatat tatccctctt gttgacatca actcttgggg ttttattggt ttcttgtgga
    23281 tctactcaaa attcatccta ttatgataac gatggaattt atagtggctc agaaaaaagt
    23341 aaatctaaag aggctgaaca agttgcacaa aataatgcaa acttggccta tcaaaattac
    23401 ttcaaatcaa aacaattaaa tgctgaagaa gaaattttta ctgatgttga aaattattct
    23461 tccgtaaccg attcaacaca aacaactgca gaaagttata cttccaatgc cagttggggt
    23521 tcaaacccaa cttcaatatc tgtaaatgtt tatgattccg gttggggatt taattattgg
    23581 aactcttggt atgcacctta ttggggattc aacacttggt atggtcctta ttggggtgtt
    23641 ggattttatc cttggtatgg agcttattgg ggctggggat atccttatta cgggtatgga
    23701 tacggttacg gatatggaag ttattataat ggttacgcct acaatagtgg aagaagaggt
    23761 ggtaactatt acaccaatgg ttacagagga agttcagctt attctagcag aacaaatggt
    23821 tacagaggaa gttcagccta tagaagtcag tatagcactc gaaattacac caattcagga
    23881 acgcgttttg gtactaccag aacagggact agaggctata acaccaacgc tgtaaattca
    23941 agcaatccta gaagtaacca atacaacaca agcggaaccc gcaataacac tagaaattac
    24001 ggaaatacac cttcaactgg aaataatact tatactccaa gaagtagtag cagtagttct
    24061 tcttcaactt caggaacaag aggtggagga tattccggag gtagcagtgg tggcggaggt
    24121 cgatctggtg gcggaggcgg aggcggacgt tcaggtggaa gaggttaatt tattatttgt
    24181 tatatagttt tagtttaaaa atatagtaat gaaaaagata ctattattcg ttgtagggtt
    24241 tgctagtcta accatacaat ctcaagaaat tcaagatgcc gttcgttaca gtcttgacga
    24301 tatgaccgga acagctcgtt ttagagcaat gagtggtgct tttggagcat taggtggcga
    24361 tttatccgcg ataaacatga acccggctag ttctgttatt tttaacacaa atcaagttgg
    24421 ctttaccttt tcaaaccata gtaaaaacaa cgagtctact tattttggca cgtatactga
    24481 acaatgtgac agtagttttg atatgaatca atttggtagt gtatttgttt ttcacaacaa
    24541 tgatgcaaat tggaaaaaat ttgcctttgc tattaattat gaaaacgaaa acagacttga
    24601 taattctgtt ttttctgcag gtgttaatcc tactacatcc atagatagtt attttttaaa
    24661 ttatgccaat ggtattcctt taggaacatt acaaagtaat ttatatgcct taaattatgg
    24721 tgaacaacaa gctttttttg gttacaatgg ttatattata agccctgtaa ccgaaaattt
    24781 gagtaatact cagtatgttt cagaggtccc tgccggcggt aattattatc aggataatta
    24841 cgtagaaagc tacggattta atggcaaagt tgcctttaac ttagcaacac aatataaaga
    24901 ctttttgagt tttggtatca atttaaatgc tcactttgtt gattttaatc gtgccagtag
    24961 tttttacgaa aaaaacacca ataatttaga aaacggtgtg caagatgtcc tttttgaaaa
    25021 ctttttatat acttatggtt caggcttttc agtgcaagtg ggaactattt tcaaagcaac
    25081 acaaaattta cgatttggag cttcttatga atctccaaca tggtacaatt taaatgatga
    25141 attcacacag cggttatatg caacgaacag agttaatggg gtaactttta atgatatagt
    25201 tgatccaaga attcttaata tttatccaag ttatgaagtg caaactcctg aaaaatggtc
    25261 cgccagtgct gcctacattt ttggtaaatc cggattaata agttttgatt atactttaaa
    25321 aaattatagt cataccaaac taaaaccgga aaatgattat gtttttagaa atttgaatac
    25381 tgaaatggca aacattttaa ataatgccaa tgaattcaga gttggtgcag aatataaaat
    25441 caaggaatta agcattcgag gaggctatcg ttttgaagaa agcccttata aaaatggtaa
    25501 aacattaggt gacttgacca gcttttcagg aggtcttggg tataattttg gctatttcaa
    25561 gttagacttt gcttatagct attcacaaag aaaatctcaa gaaggctttt ttacaactgg
    25621 acttacttcg cccgctttta tcaataccat cacaaatagc tttactacgt catttatatt
    25681 tgaactataa ttgagggtaa aatataaaaa caaaaaacca ttcgaaaacg aatggttttt
    25741 tgtttttatc aaaatttact caatataaat agctaatttt gcagtccgtt ataaaaaaca
    25801 gtatgagaac caaatcttta aagaaaaata aaatcaacgt cataacttta ggttgttcta
    25861 aaaacgtata tgacagtgaa gtgttgatgg gacaacttaa agccagtggc aaagacgtag
    25921 ttcatgaaca agaaggaaac atagtagtaa ttaatacttg tggttttatt gataatgcaa
    25981 aagaagaatc tgtcaataca attttagaat atgttgacaa aaaagaacaa ggattagtag
    26041 ataaagtatt tgtaaccgga tgtttatctg aacgatacaa acctgattta gaaaaagaaa
    26101 ttcctaatgt agatcaatat ttcggcactt cagatttgcc ccaattactc aaagttttag
    26161 gagcagatta taaacatgaa ttattaggtg aacgattaac cactactcca aaaaactatg
    26221 cttatttaaa aatttctgaa ggttgcgacc gaccatgtag tttttgtgct attcctctca
    26281 tgagaggcaa acatgtatct caaaccatag aaaaattggt aagcgaaaca gagaaattgg
    26341 ctaaaaatgg agtaaaagaa ttaattttaa ttgcccaaga tctcacctat tatggtttgg
    26401 acatttataa aaaaagaaat ttagctgaat tgctggaaaa tttagtaaaa gtggaaggaa
    26461 ttgaatggat acgattgcat tacgcttttc ctaccggttt tcctttggat gtattggaat
    26521 taatgaaacg agaacctaaa atttgtaatt acatcgatat tccgttacaa catatttctg
    26581 attccgtttt aaaatctatg cgaagaggaa caacacaaga gaaaacaact tcattattaa
    26641 aagaattccg aaaaacagtt cccgacatgg ccattcgaac aactttaatt gttggttatc
    26701 ccggagagaa ggaagaggac tttcaaatat taaaagattg ggttcaggaa atgcgttttg
    26761 aacgtttagg ttgttttgct tattctcatg aagaaaacac acatgcatat ctgttggaag
    26821 atgatgttcc ttcagaagtg aaacaacaac gtgcggcaga aatcatggac attcagtctc
    26881 aaatttcatg ggatttgaac caagagaaaa ttggtcaaac tttcaaatgt atcatcgaca
    26941 gaaaagaagg tccctatttt attggaagga ctgaatttga cagtccggat gtagataatg
    27001 aagtactaat agatgcttca aaattttatg tgaaagttgg tgattttgct cttatcaaaa
    27061 tcaatgatgc tactgaattt gatttatatg gtgaacctat ataaattata ttgtaaataa
    27121 catgctttta atcccttgac taattttcaa gggattttta atttaatact gttaaacctg
    27181 atatttatca tacactattt ccgtttttat atttaattta gttaaatcaa ataaaaccta
    27241 tattaacata taaatcctat cactatgatt actactcatc caatcgttca agaattccca
    27301 gagtatttag ataaaataaa agatttatat catcacaatg aagaatttca ggttctttta
    27361 gggtcatatc attctttaga caaagaaata tttgaaattg aaaaaggaga aattcccgct
    27421 actgatgatg aattaaccca taaaagaaaa gacagggtgt tcttgaaaga tgaaatttat
    27481 aactatttaa aaaacaacta aacttaacag ctgaaagtaa attttcagct gttttttttt
    27541 ataatgtcag taaacctatt gagcgtaagg tattaatttg tttagaatac caaaacaatt
    27601 caaaatcttc aaaatgcaat tcaaaatcag atttgatttg agaaaaagtt attgttttat
    27661 cccctgagat tgaaattttt tctagcattt ctgcaaacca aatgcctttt tctttttcta
    27721 atgaaatttc gaccgcttca gttttagaat gaaatatcag tttaaccatt tcccatgatt
    27781 tacctttttt agatttggta aatagttcat tcgaaggcct tgctcctatc caaacaattt
    27841 ttgtcgacgg tttagtgttg aatgattctt ctttttctaa acatgaaata atataatcag
    27901 gagcaatttt cgttttagga attttaaaat caaaccattc ttgtaactca tattcgaagc
    27961 aaattccgtg cataaaatta aagagcgatt tcttcaatcc aaaactgaat ttatcatgat
    28021 tgattccggt tttgtctgta aacagaatat cgttgttggc aaaagtaatg tcattttgtt
    28081 gcggaattac accaaactct tcgggaaaca ttccaaccgg actgtgagcc gtcatggcaa
    28141 attgatgcca aaacccggat tgtaaaattc ctaattcaaa taattgacga accatttcta
    28201 aactatcgat agtttcctga atggtttgtg tcggatagcc atacatcaag taagcatgca
    28261 ccatgatgcc tgattcagta agatttcggg tgacttgtgc tacttgctct acagtaacac
    28321 cttttttaat tagctctaat aatcggtcgg acgccacttc caatccaccg gaaacagcaa
    28381 tacaaccgga agctttgagc aactgacata aatcagccgt aaagcttttt tcaaagcgaa
    28441 tgttggtcca ccaactgact gttatttttc ttctaagaat ttccaaggct acttcacgca
    28501 ttaatgccgg aggagctgct tcatccacaa aatggaatcc gttttcgcca gtttgagtga
    28561 taagttcttc cattctgtcc accaataatt tggccgcgat gggttcgtat aatttaatgt
    28621 aatcgagtga aatatcacaa aaagtacatt ttccccaata acaaccgtga gccatggtga
    28681 gtttgttcca acgaccatcg ctccacaaac tgtgcattgg attggcaatt tcgattacgg
    28741 aaatgtaatt gcctaattgc aaatcggaat aatcgggtgt gccgacttct gattgtttat
    28801 agtcgggttg agtagaattg tttttataaa caacttcatt tttttcaagg agaaaagttc
    28861 ttttaaattg gatggaagag attcctcctt cgtcggaatg acaaactgaa tgatgtagaa
    28921 gttcaatcgg taattctcca tcatccaatg taataaaatc gaaaaattca aaaacccgtt
    28981 tatcttttaa atcgcgtaat tcggtattgg gaaaaccacc tcccattgct gttttgattg
    29041 ttggaaaatt tgtttttatg aaatgagcac atcgaaaggc actgtacaaa tttcctggaa
    29101 acggtacaga aaagcaaact aatttgggtt gaatatcagt tagtttctgg tttaaaacag
    29161 ataacgtaat ctcatcaata taggttgatt tttgattcaa ttgatagtat aattcgtcaa
    29221 atgaattggc acttctgcct aaccgttcgg cataccgact gaaaccaaaa ttttcatcaa
    29281 cacaagccac aatataatcg gataaatctt cgaggtataa cgtggctaaa tgttttgctt
    29341 tgtcttgcat tcccatggtt ccgaaagcat attccatatc atccaattgt tcaaaacgag
    29401 aagcttccgg cagaaaatta ccggaacaaa tttgacgagc taaggttgga ttttttcctt
    29461 gaagaaaatc tattacggca tcgatggttt gaatataatc ttgatttaga gtataaatcc
    29521 tttgacaatt ctcatcaact tgaaatcttg aacttgaaaa ctgaaacatt ttttctaatc
    29581 ctttttttga aaaaatttcc aaaatcactt ctatacctaa atccatttga aaagaggaaa
    29641 tgtttttggt atttagaaaa cctttcaaat aggctgtggc cggatacggt gtattcagtt
    29701 gggtgaaagg aggtgtgatt aaaaataaat ctttcaaagt gtaaagcttt tagcaaaagt
    29761 aagctgaatt ttttaatttt ctgtttccta accatttgtt aatccccttt tcaatacttt
    29821 tattttcgtt atttttacaa aaatttaaat cgtatgccaa ccgactgtat cagctatcaa
    29881 caatcaggtt atttttctgc tttaatcaac gattatctta atgaaaaacc ggagttgaaa
    29941 gccttatatc accgctttcc taacttggaa aattttaaat tacagttgga agaaaaaaga
    30001 aacaatttca attacaaaga caatggtaaa ttaaaacgtg aagatttagt ttctgtatta
    30061 gaaaagcaat atcaatcaat tgttgcttct gaaacaacat taaacaacat taaactttta
    30121 aacaatttaa acactttcac aattacaacc ggacatcagt tgaatttgtt taccggacca
    30181 ctttattttt tgtataaaat tgtttcaacc attaatcttt gtaaagaatt aaaaagtgaa
    30241 tttcctgagc atgattttgt acccatttat tggatggcaa cagaagatca tgactttgaa
    30301 gaaattaatt actttaattt taaacataaa aaaatcagtt ggaatcgaga aagttttgga
    30361 ccggtgggac gattatcgtc cgatggttta gaggctgttt ttgctgtttt tgaatcggaa
    30421 attggtttag gaaagaatgc ggaaggtttg aagaatttat tcaaaaaagc gtatttggaa
    30481 catactaatt tagccgatgc tactcggttt ttagcgaatg aattattttc caaagaaggt
    30541 ttagtaatta ttgatgcaga caaatctgat ttgaaaaaat cgtttattcc atatattaaa
    30601 aatgaagtgt tgaatcaaac tgcttttgcg aaagtaaatg agacctctcc tttgttggaa
    30661 tcgtattctg ttcaagtgaa tccaagggaa attaatttgt tctatattga aaataatttg
    30721 cgtgagcgaa ttgtttttga aaacggaagt tatgctgttt tgaatacaaa aatgactttt
    30781 tctgaagaag aaattttgaa agaagttgac aatcatcctg aaaaatttag cccgaatgtg
    30841 ttgttgcgac cgttatatca agaagttatt ttgccgaatt tatgttatat tggtggtggt
    30901 ggcgaattag cctattggtt ggaattaaaa tcatttttcg aaagtcagca tgtaactttt
    30961 ccaattttgt tacttcgtaa ctcggttttg ttagccactg aaaaacaagt ggaaaaagcg
    31021 gataaattgc aattgacttg gcgtgattta tttttaaatc aacaagaatt atttaatcaa
    31081 aaaaccaaat tgctttctga ttttacgatt gattttactg ctcaaaaaga tgctttgaaa
    31141 aaacaatttg aagcactaca tgaaatggct aaaactactg ataaatcatt tttgggagcg
    31201 gttaaagcac aagaagcaaa acaaatcaga ggcttagaaa acttagaaaa aagattattg
    31261 aaagccgaaa aaagagttca tcaagaacaa ttaaacagaa tcatttcatt acaaaacgaa
    31321 ttatttccca accaaagttt acaggaacga aaaataaatt tctctgagtt ttatgtggaa
    31381 tttggtgccg aattaataga aatattgttg aaggaattaa aaccgttgga aaatgaattt
    31441 aaagtagtgg ttttatagaa aaacttcaaa accaaaactt gaaacttcaa acaaaaaccc
    31501 tacctttgca cccgaattaa aaataataaa acaaaatgac tacaaataga acatttacaa
    31561 tgattaaacc ggatgctgtt gaaaacggac acatcggtgg gattttaaat atgatttcag
    31621 aaggtggttt caaaatcgtt tctttgaaat taacacaatt gactgtagca gatgctcagg
    31681 cattttatgc tgttcacgct gcaagaccat tctttggtga gttagttgaa tttatgacaa
    31741 gaggaccgat tgttgctgca attttagaaa aagataacgc tgttgaagat ttcagaactt
    31801 taattggtgc aacaaatcca gctgatgctg cagaaggaac tatccgtaaa aaatatgcta
    31861 cttctatcgg agaaaatgcc gttcacggtt cagattcaga tgaaaatgct gctatcgaag
    31921 gtgctttcca tttttctgga agagagcaat tctagtattc agttttcaat aaacattttt
    31981 gctaataaat acaaaatccc gatccttaat tgaatcggga tttttttaac tgtatttttc
    32041 cattaacgac tgaacaactt tatctatttt ttcaaaacgt tgtttacgtt caattttttt
    32101 aggaggtgct actcgctcaa aacaatctga aattgaacac gtttcacacg tgactccaac
    32161 ttttttcctt ttaacagatt catcctccaa aaactgtatt ttacttaaag aatgtgttga
    32221 aatcataatt cctaaagcga tacttcggta atatccattt ctaaatggat cttttgttgc
    32281 cgatgacaat acaaaatatt cattatccgt atgattataa ctcgatattt gagtgtcaaa
    32341 aagatgattt tcttttgaac ttggaatctc acttaaggtc ttaatagaaa tccaccgtct
    32401 gcaataatgt tcgttatttt cattagcatg cggctctaat aaatttgtaa tatgcaactc
    32461 tttggttaac tgaaaatcat tacgttccgg tttataattg aatcgcaaga aaaatatatt
    32521 cttcaagtta aaatccttgg gcaaaacatt ggtcaaacgt tgataaaagg tctccggtga
    32581 atcggtaaat tttgaaatta aacttgtaaa atcagttgga ttgaacgtct tttgctcaaa
    32641 aaccaatttc aattctttca ccaattcctt tctcggaatc aacaaagctc cggcaaaata
    32701 cgaggcaaaa aagttgttca aaacctgatc aaaactttca aattttatcc atgaaaatgt
    32761 atacaatcga tcgctaatct ccatataatt gtaagcgatt tcttttgcat aaataaacat
    32821 tcgttgcgaa atatctgcac ttttggaaat caacaacgtc tttgatttag gtacaaaaac
    32881 agaacgcaaa tacaccaact cctcatgttc tgtcaactct tcattattta tggtgtaacc
    32941 atattcatca actaaaattt cttccaaatc agcaacgttc aacttcttgg ttaaatccaa
    33001 cgaataggct ttggcaaact tctcaacttg caattcaatt tcgtcaaaat aattattgtt
    33061 tgcttcctga taggaacgca aagctgccaa aaaaaaactc tctctagtaa gattgtaatg
    33121 cttcgcaatt tcaaacaacg tactgataaa agcgttcact tttacaggag cttccgcaat
    33181 aatatcaatc aaattgctct ctttaattcc gaaaatttca agcggtatct cttttaaaat
    33241 tcgagattgc aagatttcac caatcggagc taaattctta tccaatttca aggaaaccat
    33301 ttggtcataa gttgaatcca atgcctccga taatagtaaa attttatctc ttttcggata
    33361 ttttttccct ttttcaatct cgttcaaata cgatttagaa agtccggtaa ctttagctaa
    33421 gccaaacaac gaaagatttt tctcagttct gatttgtcgc aatttcaaac caaatatcaa
    33481 tctaatatat tcttcttcta tcatattata taaaaaatat ttgcttcaat caggaccaaa
    33541 aacatcctca atcgtaacca ttttctaaaa acaaatatac aacaattcaa ataataacaa
    33601 ctgttgaaaa aatattttta cattttagcg aacgttcgct tgttttgtgt ttattttttt
    33661 tatagtattg cagtaacaaa aaaagcacct tatggaaaca gtagaaaaaa caaccgtaga
    33721 agttcttaat ccaacagaac accaaagcat cttaacagcc gaagcattga cttttttgag
    33781 tttattacat caaaaattta atttaaaacg aattgaatta ttaaagaata gagaaacaca
    33841 gcaagaagta tttgaccaag gcttttcacc aaaattccca aaagaaaccg aatcagtaag
    33901 aaattccgtt tggactgccg caccaattcc agaaaaatta ttagatagac gagtagaaat
    33961 aactggccca gtagatagaa aaatggtaat caacgcacta aattcaggtg ccaaaatttt
    34021 tatggctgat tttgaagaca gttgttcacc aactttcgaa aatctaatgg aaggacaaca
    34081 aaacctaatc gaggctatca gaaagactat tacattagaa cttaatggta aaacatataa
    34141 actaaataaa gatactgcaa cgctacttgt tcgcccaaga ggattgcatt tagaagataa
    34201 aaacttaatt tgtaacgaag aaaatctttc cggttcgtta gtagattttg ggttgtactt
    34261 ttttcataac gtagtagaac ttcaaaaaca aaatcttggt atttatctat acttaccaaa
    34321 aatagagcat tacacagaag cacgttggtg gaacgaagta tttgaatttg ctcaaaacta
    34381 tttcaaaatt ccaattggaa ccatcaaagc aaccgtttta attgaaacca ttacagccag
    34441 ttttcaattg gatgaaatca tttatgaatt aaaaaatcat atcgccggat taaattgtgg
    34501 tcgttgggac tatatttttt cctatatcaa aaaattaaaa agccaccctg attttatggt
    34561 tcctgacaga gaccaagtaa ccatgaccag cccgttcatg agtgcttatt cattaagagt
    34621 cattcaggtt tgccacaaaa gaaatatcct ggcaattggt ggaatggcag cacaaattcc
    34681 cattaaaaat gatgaagaag caaacaaaat tgcttttgaa aaagtaaagg ctgacaaaga
    34741 acgtgaggtt aaaaacgggc acgatggtac atgggttgca catcccggat tagttagttt
    34801 ggcgatgaaa gtatttgatg aaaacatgcc aacaaaaaat caaatgcaca taaaacgtgg
    34861 ggagttaaac atcaccgaag agcaattgct tgaacttcca aaaggtacaa tcaccgaaaa
    34921 tggagttcga aaaaacatca atgtcggaat tttatattta gaatcttggc tcaacggaac
    34981 cggagcagct gccttataca atttgatgga agatgctgcc acagcggaaa tttcgaaagc
    35041 acaactttgg ttgtggttaa aaaaggaagt aaagctagat aacggaattt tagtttcaaa
    35101 aacactttat gagaaaatca tccaagaaga attggtgaaa atcgaagaat atgtaggagc
    35161 tgaacgcttt aaatcaggtc aatttgaatt agccacaagg cttttctcga acatgattaa
    35221 tagtgaaaaa cttgacgatt ttctaaccct tgaagcttac aactacataa aataaaactg
    35281 ccgaatgctg taacattcga cagtttccaa ataaataatt aattaaataa ctatctaaac
    35341 agtacaaaag tatgaaaaca gaagacagaa ttcaagcatt aattacagat tggacaacca
    35401 acccaagatg gaaaggaatt gaaagacctt atactgccga aaaagtagta accttgcaag
    35461 gttcctatca aatagaacat tccatcgctc gtcgaggttc agaaattcta tggaaaaaat
    35521 taaataatca agattgggtt gcaggacttg gagctttaac aggaaatcaa gctattcaag
    35581 aagttgaagc cggtttagaa gccatttatc ttagtggatg gcaagtggct gctgatgcca
    35641 atattgccgg agaaatgtat ccggaccaat ctttatatcc tgctaatagc gttccgatag
    35701 tcgttaaaaa aatcaacaat gctttattgc gtgccgatca aattcaaact gtaaataaaa
    35761 ttgaaaacaa aaaagattat ttagttccga tagttgctga tgcagaagca ggttttggcg
    35821 gtaacttgaa tgcttttgaa ttgatgaaaa gcatgattga agccggagca tcaggcgttc
    35881 attttgaaga ccaattatct tcagcaaaaa aatgtggtca tttgggtggg aaagttttgg
    35941 ttccaactca agaagctata aacaaattaa tcgctgctcg tttagctgcc gatgtgatga
    36001 atgttccaag tttaattgtg gctcgaactg acgcagacgc agcaaattta ataaccagtg
    36061 atgttgacaa acgcgataga aaattcatca caggagaaag aactccggaa ggcttctttt
    36121 tcgtaaattg tggtgtagaa caaggaatag accgaggatt atcttatgct ccgtatgccg
    36181 atttaatttg gatggaaact tcaaatccag atttagaata cgctcgaaaa tttgcagaag
    36241 gaatacatgc caaatttcca ggaaaaatgc tagcttacaa ttgttcacct tccttcaatt
    36301 gggccgcaaa attatcagtt gcagaaatgg aaacttttag agaagaatta gctgagatgg
    36361 gttataaatt tcaatttatt actttagccg gattccatgc cttgaacacc tccatgtttg
    36421 aattagcatt agcatacaaa aaacgtggaa tggctggata ctctgaatta caagaaagag
    36481 aattcgcact tcaaaaagaa ggatttaaag ccgtaaaaca ccaaaatttt gttggaacca
    36541 gttatttcga cgaaattcaa aacacagtta cagcagggct ttcatcaaca attgcaatga
    36601 aagattctac agaaaaagca caattttagt ttaattaatt ttttgttctg gttaatcatg
    36661 aaaactctcg gaaacgggag ttttttttta tctcaacacc aactccttca ctacatcttt
    36721 tcgtaattca tcgttgagca taattatgat tttttgtttt ccatgactga
//
LOCUS       DAOTCW010000008        33030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_664_length_33030_cov_28.439939, whole genome shotgun sequence.
ACCESSION   DAOTCW010000008 DAOTCW010000000
VERSION     DAOTCW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 33030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33030
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_664_length_33030_cov_28.439939"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="P5335_01365"
     CDS             <1..523
                     /locus_tag="P5335_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73557.1"
                     /translation="IKLDLLNARKNEISAKQFVKEFKQITEKSCLCVGLANSSYLEMG
                     IPIKGQSQGVVVCPGPNLAYFNKEVSLFNMVQHIYGKTSDLVPTNRPHFFIKELSLYI
                     DYFKKEIDVFEEATSQQLKKVNQFKSNLLEGITYYKILFEKSHYFTDLKSKIFHELNQ
                     FELELNKEKEVLV"
     gene            880..2343
                     /gene="nirK"
                     /locus_tag="P5335_01370"
     CDS             880..2343
                     /gene="nirK"
                     /locus_tag="P5335_01370"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HRZ73558.1"
                     /translation="MKKYFVKMMFLSISILLIYSCKNEKTNLDYSSIKVSGEIIAELT
                     APPFVPKPVGNRPAQKLIVNMEIKEIEGEMADGVKYVYWTFGGSVPGSFIRTRVGDEV
                     EFTLKNHPDNKLPHNIDLHAVTGPGGGAASSLVAPGHEKTFNFKCINPGLYVYHCATA
                     PVGMHIANGMYGLILVEPEGGLPPVDKEYYVMQGDFYTKGKYGEPGMQPFDMTKAVDE
                     HADYVVFNGKVGALTGEGALTAKVGETVRIYMGNGGPNLVSSFHVIGEIFDKVHIEGG
                     DAINKNVQTTLIPAGGSAIVEFKVDVPGTFILVDHSIFRAFNKGALGMLKVEGAENAK
                     IYSGTTQEGIYHPEGGTIQNMPKTGNGKEVVVNKTLDQQLTDGKNIYSRTCFACHQSE
                     GQGIPAAFPPLSKSDFLNADPNRAINAVLHGLSGEITVNGKKYNSIMTSQNLTDQEIS
                     DVLSYVFNSWGNNKTRITPEMVKKERAKPAPKAVDMH"
     gene            2482..2793
                     /locus_tag="P5335_01375"
                     /pseudo
     CDS             2482..2793
                     /locus_tag="P5335_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
     gene            2819..3604
                     /locus_tag="P5335_01380"
     CDS             2819..3604
                     /locus_tag="P5335_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRZ73559.1"
                     /translation="MEAQFIKRFHYCLILLFWVSISFSQLPKNMVTIGEGNYIPLYGT
                     SDKKPVTIKSFYLDIYPVTNQEYLIFLKTNSDYRKSTIKRLFANTTYLSEWKSDLNFG
                     ELNPSAPVTNISWFAAKKYCDCQGKRLASLDEWEYVAMADEKRKDARSREEFNKYILS
                     WYEKNKTYNISVGKTFKNYWGVYDMHGLVWEWTFDFNSIFLSGESRKDKDTDKDLFCG
                     SGSVNATDLMNYAAFMRYAFRGSIKANYTTKNLGFRCAKNISN"
     gene            3626..4219
                     /locus_tag="P5335_01385"
     CDS             3626..4219
                     /locus_tag="P5335_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRZ73560.1"
                     /translation="MKKIIFSLITSLLILGCNSKEENKEIKDKPITDLSIYNLPEKWT
                     NQDGEDIELKELRGKVLVMVMIYTSCKAACPRLVADMRHIEEQIPKEYKDNVQLVLVS
                     IDPKIDTPERLKEFSIENKMIGNQWVFLRSNEENTREFAAVLAVNYKKISPIDFSHSN
                     IISVFNAEGELSFQQEGLGVSYNKTVSKIIEEATKLE"
     gene            4250..5527
                     /locus_tag="P5335_01390"
     CDS             4250..5527
                     /locus_tag="P5335_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRZ73561.1"
                     /translation="MKPYNQFFVVIAFAFTPISSFSQELEVNLQIRPRYEYRNGFKEL
                     MKDEFYPTSFVSQRSRMSFFFKHEKFKANLSFQNIRVWGDVTTTSSSDKNGIAIFESW
                     VSYYFNDNWSTKFGRQVLSYDNQRIMGGIDWLQQGQSHDAVLVTNKKNNYQLDFGLAL
                     NNNNETLYESAYVTNYKNMQFIWFNQKFSKLNLSILALNNGYQYENLDENKFKIAYLQ
                     TFGTYGKWDTSKFYGDFGLYAQTGDLAIINDKVDVAAYYAGFNLGYKFTTSFKIELGF
                     EYLSGKDQNDSDTKVKSFSPLFGTNHAFNGLMDYFYVGNHKNTVGLQDVYSKFFFEKN
                     KWQITLAPHLFYSSADIYNSDTLEIQENYLGTEIDITGSYKMDKNFLISGGISKMFAS
                     TSMEILKGGNSSNCNNWAWVMVSFTPQLFTYKK"
     gene            5794..6225
                     /locus_tag="P5335_01395"
     CDS             5794..6225
                     /locus_tag="P5335_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRZ73562.1"
                     /translation="MLSKSQARTFFLGGTVVTFLVFIGLTIYSFMPKNDQTYHEKIDA
                     KVIRGKEIWESNNCMGCHTILGEGAYYAPELTKVVERRGEGYIKAVLQSPVPWGPKGR
                     KMVKYEMNDADAEAMIAYFKWIGNIDLNGFDRVVSPLAKDK"
     gene            6245..7579
                     /locus_tag="P5335_01400"
     CDS             6245..7579
                     /locus_tag="P5335_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRZ73563.1"
                     /translation="MKYKSQKVAYWFFALCMLLFALQITYGFIMGFARIGMDGLHDFI
                     PFNTARAVHTNLLVVWLLTGFMGAAYYIIPEEAQRELINVKLAYVQLISLAIVGVLAI
                     IGYHFNIWEGRKFLEIPRELDYLVVINVLLFLGLILGTLYKAERRTTTALVLSMGLLF
                     AALLYLPGMIWFDSQVTDSFFRWWVVHLWVEGVWELIMGGILSYLLIKLTGVDREVIE
                     KWLYVIVGLTFLSGLLGTGHHYYYIGVNKIWLIVGGIFSALEPLAFLAMALFAVYMYR
                     KGEKSHPNKIALFWTIGASIVSFVGAGLLGFAHTLPQTNIYTHGTLVTAMHGHYAFWG
                     AYAMIVLAIISYALPNMTGRKRYNNTIGHMAFWLSNIGMLGMTVAFGVAGVAQVYLER
                     KMKMDFMDVQNEISIHFVVLLLCATLFTVGISMYIYEFIKYGKPTDEALVKE"
     gene            7683..8468
                     /locus_tag="P5335_01405"
     CDS             7683..8468
                     /locus_tag="P5335_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HRZ73564.1"
                     /translation="MIEVEKTIFYKAVGSEIEIFNHINQLQLPLLLKGPTGSGKSRFI
                     EYMANELNKTLVTVSCHEETSATDLIGRFIIKGAETIWIDGPLSIAVKNGFILYLDEV
                     AEARPDVIVAIHSLTDHRRELFIDKLGETIKAHPDFLLVASFNPGYQKGFKELKPSTR
                     QRFVALTFDYPNPKIESEILINETGIQADIAQKLVAIGTKIRNLTELGLIETVSTRLL
                     VDAAKLIKNGLPKRLAVHIAVVEPLTDEHETIQALNDLCDLMI"
     gene            8481..10247
                     /locus_tag="P5335_01410"
     CDS             8481..10247
                     /locus_tag="P5335_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRZ73565.1"
                     /translation="MGFELDEIIYKKVLKYFKNKRLNDVELLSRQINLSDIKPRLTLF
                     ARAISGAPIEIFPAEREGGYKNKSFFLPISSTLFPTKQENLKFYFFRIVYLSVQKQLN
                     LNWENQEQTHQLSLEKAVETAPLVLAKMFQDFPSMQEFYYDAVPKLPVNKKDQTIDYS
                     WLYGKWMKNTLEIEDENVLKNFDDATKKLQNTVVAETTLHAKAVEEIESLTIDKKQQE
                     DYVLMHSFEKIETAEEFNGNWRDFDGADDLKQHQEALEEMNMKFTVRVDDMVHSVYQA
                     DFVENTSIAESAEVDEKGFHLKYDEWDFKKRNYLTNFCKVYPKTQLKTHATYYKKTMA
                     KYKTTLNGLRKMLTSVNNKMQQQRRQLQGDTFDLDALTDLYTDIHSGKSPDERIYLSQ
                     RKKNKDIAILVLLDISLSSDGYAAGNRVIDVEKEVSILFGEILHEFDIDFAIDGFYSK
                     TRNFTTYLTLKDFNESWNKAKHKIGAVEPNGYTRIGPALRHAGARMDQLDAKNKWIIV
                     LSDGKPNDYDKYEGQHGIQDIKQALRELNERKINSYALAIEAQAKYYLPQMFGQNHYQ
                     ILTSPEALLKSLVKLYEKIKHQ"
     gene            10249..11217
                     /locus_tag="P5335_01415"
     CDS             10249..11217
                     /locus_tag="P5335_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRZ73566.1"
                     /translation="MSVSETPTIVIEGHPVNTYFLESELIHQLLEEIKAINPKEDFQK
                     FYNVFNHLAMIEKRFERKENQLFPFLEQKGWTGPSRNMWSFHDTIREMFRIVRKNLEE
                     QDFTSAKHNTNLITQNLYRLLDVEENVLFPNALELLSEEDWIKMRNGEVEIGWMLAEA
                     PAKYPKESEYIHPSHDTERRTDVIFDEKAAHYDEGYMTIEQVNLLFKTLPIDITFVDE
                     NDKVIFYNRGDERVFPRSAGIIGREVRFCHPPKSVDTVLKILEAFKKGEQNEASFWIN
                     YKEQLVYIRYFAVRDSQKNYKGVIEMSQDITDIKQINGEKRLLEWN"
     gene            11208..11780
                     /locus_tag="P5335_01420"
     CDS             11208..11780
                     /locus_tag="P5335_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRZ73567.1"
                     /translation="MELKEKSIYYPPGGILIWIIIYLELITFGMAIIALAYYGSEERD
                     LFHNNSLKLNKTIGTINTILLLTSGFFVAKGIQYFKVQHIKKTLLFFNWAIVGGFGFL
                     VLKSIEYYEKLKVGLTMDYNSFFTFYWLLTGFHLIHVIVGLVLLIVISFSIRKKQIEA
                     SFEDIEASASFWHMCDLIWLLLFPVLYLLF"
     gene            complement(12281..12724)
                     /locus_tag="P5335_01425"
     CDS             complement(12281..12724)
                     /locus_tag="P5335_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008635428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRZ73568.1"
                     /translation="MFSKSCEYAIKAAIFIANNSLVDKKVGFVDIAKMIDSPEAFTAK
                     ILQKLTKDGVILSVRGVNGGFYIPKNLIAKVKLAHIVQAIDGDAVFKGCGLGMARCNE
                     KHPCPLHEKFKSIRNNLQQMLESTNLEELTLGLKSGETFLKTKKN"
     gene            complement(12798..13238)
                     /locus_tag="P5335_01430"
     CDS             complement(12798..13238)
                     /locus_tag="P5335_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73569.1"
                     /translation="MKTFIRLMILLLIVGFYSCKKNEQQKVIVEETEVEHDADFQFPA
                     VSLNDGQLWEANTETTQGIKNMQLLMDSYSKENGNPEELISGLKAEFEMIFKKCTMTG
                     EAHEQLHNYLILLKTKINSLSDGDSNEYRTNLKNYLEDYFNYFQ"
     gene            complement(13315..14037)
                     /gene="ric"
                     /locus_tag="P5335_01435"
     CDS             complement(13315..14037)
                     /gene="ric"
                     /locus_tag="P5335_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HRZ73570.1"
                     /translation="MNTVEQLTVGEYVANDFRTAAIFSKYGIDFCCKGNRTLEEVCDK
                     KNLNPTDLEREIQTVLETKNDNAIDFKSWSSTLLIDYIIEKHHSYVEDKSPVLLQFLD
                     KLCKVHGDRHPELFEIDSLFKLSAGALAQHMKKEELILFPYIKKMVIAQKENQPIQQS
                     HFGSVENPIAMMKQEHESEGVIYAKIAELSNNYTTPEDACNTYKVTFSMLQEFELDLH
                     KHIHLENNILFPKAIALQEQFQ"
     gene            complement(14136..14507)
                     /locus_tag="P5335_01440"
     CDS             complement(14136..14507)
                     /locus_tag="P5335_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRZ73571.1"
                     /translation="MKKDIHNRNDIIRLVDAFYDKIKTDQVIGFIFNEVAHVNWENHL
                     PKMYDFWENVLFFTGNFDGNPMQKHKELHNKCPLSHAHFDHWNEVFRQTVDELFKGEK
                     AEEIKLRAKSISDVIMYKTIQ"
     gene            complement(14527..15294)
                     /locus_tag="P5335_01445"
     CDS             complement(14527..15294)
                     /locus_tag="P5335_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRZ73572.1"
                     /translation="MNRIIKIILTDILKNKIVLVYTIVLSLLSWSAFGLEDNSAKGLL
                     TILNVILFTVPLVSILFTTIYLYNSSEFIELLLSQPIKRNKIWQSLFFGLSASMVLAF
                     FIGAGIPLLINAPNAVGLMMILIGCLISVVFIALAFLCSILTRDKAKGIGFAILTWLY
                     FALLFDGLVLFLLFQLSEYPIEKAMVAVTALSPIDLARIQILLHLDVSAMMGYTGAIF
                     KDFFGTTAGLFASFGLLILWVVIPFYFSLRKFIRKDL"
     gene            complement(15291..15998)
                     /locus_tag="P5335_01450"
     CDS             complement(15291..15998)
                     /locus_tag="P5335_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRZ73573.1"
                     /translation="MIEIKNISKKFGKLNVLHDVSLSCKKGECIALIGPNGCGKTTLI
                     KSILGMVLTDEGFINFNGKSIVNEYLYRSNIGYMPQIGRYPDTMTIGGVVEMIKAIRN
                     SNQELDEELWHSFELDKMADKQMRTLSGGTTQKVSATLAFLFNPEVLILDEPTAGLDP
                     LASEILKDKIIKEKEKGKLILITSHLLSELDDLVTEIIFMQEGKVHFHKKVNDLKTET
                     NETKISKAIASILKQKK"
     gene            complement(15995..17224)
                     /locus_tag="P5335_01455"
     CDS             complement(15995..17224)
                     /locus_tag="P5335_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HRZ73574.1"
                     /translation="MKFFLFILFFISLSLKATTIHIGKNHPIQSIKKGIALAKDGDTI
                     IVHGGWYREGNIIIDKKIVFLGKNLPVLDGEKKHEVLSIHADSVVVDGFKIIKCSYAT
                     ITDPCGIKVYNRSHVLIQNNVLDDNFFGIYLQNCQNTTIKNNYIKAYGKEEQKLGNGI
                     HCWKSNNILIIANKIDGHRDGIYFEFVYDSLIWRNISTNNVRYGLHFMFSHSDTYITN
                     LFRNNGAGVAVMYSKDVKMFNNTFDENWGASAYGLLLKDITDSYIFNNKFNANTSAIY
                     VEGSSRNLVEKNQFNANGWAMQIQASSMDNVVVNNNFKGNTFDIGTNGSLVLNSFNNN
                     YWDKYEGYDLNRDGIGDVPYHPLSLFAVLVEKNPSAMLLFRSFMITLLDKSEKVLPSI
                     TPDNFIDHSPLMKPLQL"
     gene            complement(17231..17650)
                     /locus_tag="P5335_01460"
     CDS             complement(17231..17650)
                     /locus_tag="P5335_01460"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HRZ73575.1"
                     /translation="MKSVYFFLIMMLTVSCATKEADPIKLNIDGCEYCKMKIADGKFG
                     AELITAKGRIYKFDDMHCMVNYHKENSSVKVQSFYIHDFNQMNVLIPAETAFYAQGGE
                     INSPMHGNIIAVKTEDEAKNMATEFKANLTSWNEIIK"
     gene            complement(17651..18235)
                     /locus_tag="P5335_01465"
     CDS             complement(17651..18235)
                     /locus_tag="P5335_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73576.1"
                     /translation="MKKNNLTLLSKVLLVIVGFLFIGALFVPMWRIELSAPQYPEGLM
                     LQLHANKIGGDVDIINGLNHYIGMATLHTENFFEFTVLPYVFVAFALISLALVFINNR
                     RAVFGFFVTIILFVILAGVDFYRWNYEYGHNLDPNAAIKVPGMSYQPPLIGYKQLLNF
                     GAYSVPDIGGWMLIGAGILLFVIVLKEFKFKKAI"
     gene            complement(18387..20369)
                     /gene="nosZ"
                     /locus_tag="P5335_01470"
     CDS             complement(18387..20369)
                     /gene="nosZ"
                     /locus_tag="P5335_01470"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009745144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HRZ73577.1"
                     /translation="MKTKFLKITSAVVLIAAFMGSCKPKNTSDTVSGDAAQKVYVAPG
                     KYDEFYNFVSGGFSGQMSVYGLPSGRMLKVVPIFSVDPESGWGYSEETKPMLNTSEGF
                     IPWDDQHHLELSQTNGEVDGRWIFANANNTPRIARVDLKRFKTAEIIELPNSAGNHSS
                     PFITENTEYVVAGTRFGVPGDYEDGDVPIVTYKENFKAHISFVKVDKESGKMDLAFQL
                     RLPGVNFDLSHAGKGKSHGWFFFTCYNSEQANTLLEVNASQRDKDFILAVNWKKAEEY
                     IKAGKGKIEKTKYAHNTWNDETHTGTSVIKEEVLVLDAMAMKDICYLMPCPKSPHGCD
                     VDPTGEYIVGSGKLAALIPVFSFDKMTKAIAAKNYEGDYEGIPVLKYDSVLHGEVQKP
                     GLGPLHTEFDGKGNAYTTFFVSSEVVKWDIKSLQVLDRVPTYYSVGHLCIPGGDSKKP
                     FGKYLIAYNKITKDRYLPTGPELTQSAQLYDISGDKMQLLLDFPTIGEPHYAQAAPAD
                     LIRNNGQLKIYDIKKNNHPRVAKGEKETKVVREGNKVHVYMTMVRSHFSPDNIEGIKV
                     GDDVYFHITNLEQDWDVPHGFSIRRAQTAELLIMPGETSTLNWKPTKVGIVPFYCTDF
                     CSALHQEMQGYIRVSPAGSNVPLSYSLGTNLPATSK"
     gene            complement(20408..20893)
                     /locus_tag="P5335_01475"
     CDS             complement(20408..20893)
                     /locus_tag="P5335_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRZ73578.1"
                     /translation="MNKLYALALGLLLFTSCGKDKTNAGEDFSKSSSTETSSETSSDP
                     ASYDPKRGEGKFTTVDLGTGLDVAMAAEGEKVAGVKCMSCHKLTDERLVGPGWKGVTE
                     RRAPEWIMNFATNPDPMIDKDPELQAQLELCLVRMPNQSLTDDDARHVLEYMRQNDGV
                     K"
     gene            21016..21378
                     /locus_tag="P5335_01480"
     CDS             21016..21378
                     /locus_tag="P5335_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73579.1"
                     /translation="MKYFIIIVTFFFLFRPIFPVFNYVVNYDYISKELCENKEKPELK
                     CNGKCHLKADLAKASEENIPASQETKSNITLELLFLETFPEFKFNRIYVEKQQVSISY
                     SRLYFRLHSTSIFHPPTA"
     gene            21540..22379
                     /locus_tag="P5335_01485"
     CDS             21540..22379
                     /locus_tag="P5335_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73580.1"
                     /translation="MKIYKIFLLFALALFTSCSSDDDSTPVNELDGLVKVKEITNDTH
                     VVELYTTSGTTQQGYNDITLRIKDKTNNEYVKNASITWMPIMHMTMMSHSCPFSMVEK
                     VVGKETLYNGSIVFQMAQNETEYWDLKIDYSINEIDYSVTDIIDVPATEKRVVSSFMG
                     TDNIRYIVALVDPKNPKVALNDMTVGVFKMENMMSFPKVNQYKLKIDPRMPSMGNHGS
                     PNNVDLTQSASTGLYNGKLSLTMTGYWKINLQLLNSENTVLKGEEVTETNLESSLYFE
                     VEF"
     gene            22434..24431
                     /locus_tag="P5335_01490"
     CDS             22434..24431
                     /locus_tag="P5335_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ73581.1"
                     /translation="MKNQFYVVFVLLTTTLSLAQEQVKDSILPVNLTEVILIGKPTQL
                     NEKQVKPLASLDEYLQQSGRIDMIKRGSYAWEPMINNMATERTIVTIDGMRIFGACTD
                     KMDPVTSYVEISNLSEATIASGQEGSCHGPTIGGSLDLKRLRSNQANLGWSGSLSSGF
                     EFNNKQRILGGMLHYRDSSMYADANLTYRKADNYYAGNEEEINYSQFQKMNASIVSGY
                     QFQNKLIEGSLIFDEANDVGYPALPMDISLARAFITSLKYEVAPKSGLLNHWENKLYY
                     NTITHFMDDSKRPDVPIRMDMPGWSDTYGYYSKGKATKEKHHLQFNVNGFYNRSIAEM
                     TMYPNDPNENAMFMYTWPDVRTLYNGMYLEDELELSCHNSLKLNGSFGFHQNKVANEF
                     GLESLQIFYPEMADQQNRILKSISLQYKHQIKDWNFSVNSGYGERAPSVTEGYAFYIF
                     NSFDLFDYIGNPNLSNEKSIEGSFDVWFKNEKIKTGITFSAFHLLDYIIGIPDENILP
                     MTIGASGVKIYNQLTYANLMHSSFQFDYRLTEYLTWNNKLQYSYGKDSEERNLPFISP
                     LQYQSQLQFKKNLFQTEVNVRGNATQTNYSPYYGENSTPDYAILNFSAGYNFKWTSQK
                     LLVNAGVENIFDTYYSTFTDWNNIPRMGRNVFINLSYAIGN"
     gene            complement(24403..25734)
                     /locus_tag="P5335_01495"
     CDS             complement(24403..25734)
                     /locus_tag="P5335_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRZ73582.1"
                     /translation="MKSGTWLLRLALLLLFLGLFFGFLASFAYRLTDAKSGMLGFLTL
                     RPLHVSSAYFGVVLGGLACVTLAIQKETVKRISFNLLKIHLFLWLITLVGIFYSYFTG
                     DFGGREYWEFKPIWALPILISYFVFLLFYLKNAGSYKNWPVYKWMWFTGIVFFSFIFM
                     ENYLWIFPYFKEHLMADMTIQWKVNGSIVGAVNQMIYGLAFYLMTKISGDEKMGQSKL
                     AFAMYFLGLFNLMFNWGHHIYILPTDRYIHHISYIVSMTEWIILIRIFYNWNQQLKEN
                     RTHYYFFPYRFLMAADYWVIINLTIALFMSIPAFNLYTHGTQITVVHAMGTTIGINTM
                     MVLAGAFYFIKPAFESLKALKRMSMVFWISQITLFLFLATLTGMGIQRALWQAGITEG
                     VFGQMMQNSRPWMIGFIITGTLLFASLGSVVLYLLFYSFKEKVSFRLHKKD"
     gene            complement(25731..26165)
                     /locus_tag="P5335_01500"
     CDS             complement(25731..26165)
                     /locus_tag="P5335_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRZ73583.1"
                     /translation="MPLIKVSPLKIFITLSVLFLIYSFTIYLKPLREKDHQKIAYQKQ
                     SASGKDVWQKHNCHTCHQLYGLGGYLGPDLTNVTAKPGYSDVFLKAIISNGISQMPAF
                     DLTNREMEDLLVFLKAMNETGTANPRHYSTQINGTFTLDNLE"
     gene            complement(26152..27693)
                     /gene="nadB"
                     /locus_tag="P5335_01505"
     CDS             complement(26152..27693)
                     /gene="nadB"
                     /locus_tag="P5335_01505"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRZ73584.1"
                     /translation="MKTCDVLISGTGIAGLTLALHLIEKQPDIKIIILSKSDPQLTNT
                     SLAQGGIATVYNQITDSFEQHIQDTLNAGQGKCNKKVVEMVIKQAPQRLNELMQWGAE
                     FDVDPKGVLDMALEGGHSQNRVVHFKDKTGWHIQTVLWKKILAETNIEVIANAFCVDV
                     ITDDRTDEKQAIGMTYMLHEQPEEQQIFANAIVLATGGSGQLFAFTSNSDVATGDGVA
                     MAYRAGATITDMQYIQFHPTALYEKGKSNLFLISEAVRGFGAYLLNSSGKRFLFDYDV
                     RGELATRDIVSEAITKEIKQAKEDFVCLDIRHLDVLDFQNHFPTIYSKFKELGYNPET
                     DLIPIVPVAHYQCGGIAVNEFGQTDVKNLYALGECSCTGLHGANRLASNSLLEALVYS
                     YNSSLQIILEFNKMIPNETTIQSRTVASHEIICPTVVSNFKNQVKHLMTYEVLHGSQE
                     EKMKALTQLIKIHTDLMECLDYYYRCRNCCELENMLQTAMIIINQSSVALHLETNKLT
                     EYAFN"
     gene            complement(27698..28630)
                     /gene="nadA"
                     /locus_tag="P5335_01510"
     CDS             complement(27698..28630)
                     /gene="nadA"
                     /locus_tag="P5335_01510"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRZ73585.1"
                     /translation="MKVIDKWSDKIQKLKKEKNAVILAHYYQQPEIQEIADFVGDSLE
                     LSRKASQSKADIIVFAGVYFMAETAKILNPTKKVLVPDINAGCSLADGCLPDEFRRFL
                     KKYPNHEVVTYINCSAEIKTMSDYVCTSSNAIKVINSIPIDKNIVFAPDKNLGKYLIK
                     ETGREIILWDGSCIVHEAFSLEKLIDLYKKHPDALIIAHPESETHILKTAQYIGSTSG
                     MINYVKTAPETKFIVATENGILHKMRQEVPDKILIPAPSKEDNTCACSECAFMKMNNL
                     EKLYKCLLEESPEVLLQEDIIIKALKPIERMLQI"
     gene            complement(28671..29105)
                     /locus_tag="P5335_01515"
     CDS             complement(28671..29105)
                     /locus_tag="P5335_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRZ73586.1"
                     /translation="MFSKTCEYAIRATIIIASESQLGNKVSLREIARKIDSPESFTAK
                     ILQKLAKNKLVESSKGNGGGFFVSKEMLSRVKLGQIVLAFDGDAVYNRCSLGLQDCSE
                     IKPCPFHNQYKPIREQLKKALDSTDLMNLISGIKIGETYLKI"
     gene            29282..30526
                     /locus_tag="P5335_01520"
     CDS             29282..30526
                     /locus_tag="P5335_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRZ73587.1"
                     /translation="MNYWKKLTAAERIARVQQALHQNVDFSKDASLGYPASKLDGKVF
                     YDDAPFLKDAPVLSTYVANPNHIGCHTTGTSETAFKGTQEIERETLDILAIDIFGSKK
                     NEFDGYIASGGTEANIQALWLYRNYFMKTFGANPSEIAIIASDDTHYSIAKGSNLLLI
                     DWINVPVGFENRKIEVDSFETKLLISVSKGKKYFIVVCNMGTTMFGSVDNPENYTKIL
                     EKHNLAFKLHIDAAYGGFVYPFLSQETILSFKNPKVSSITVDAHKMLQAPYGTGIFLC
                     RKGLIENVLTKEAEYVEGMDLTLSGSRSGANAVAVWMILATYGPNGWFEKVKVLQMRT
                     DWLCHQLSDLNIPYFREPNMNIVTIKSSFIPEKLAHQFDLVPQKHTAENEWYKIVVME
                     HVEVDHLSKFLEKLKKSMVIHS"
     gene            complement(30528..31211)
                     /locus_tag="P5335_01525"
     CDS             complement(30528..31211)
                     /locus_tag="P5335_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRZ73588.1"
                     /translation="MKPSAKKIVHSITAFTLLDYPDKTACILWFAGCNMKCSYCYNPE
                     IVFGKGSYSIPEIISFLKKRKGLLDAVVFSGGECLLHKESIVLMQLIKEMGFLIKVDT
                     NGSKPDRLSEAIDQKLIDYVALDFKSTKEKFKFITESNLYEKFLMSLAILNQSKIPFE
                     IRTTVHSELLGKNDLDEMISVLQTNNYAGNYFIQPFRNGVETIKNMNASKHKAFTDLS
                     TADIWIQMR"
     gene            complement(31195..31362)
                     /gene="nrdD"
                     /locus_tag="P5335_01530"
     CDS             complement(31195..31362)
                     /gene="nrdD"
                     /locus_tag="P5335_01530"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRZ73589.1"
                     /translation="MNTTATKIVLEQHNELRTKCLVYTRVMGYHRPVESFNIGKKGEH
                     KQRTHFNETKC"
     gene            complement(31359..>33030)
                     /locus_tag="P5335_01535"
     CDS             complement(31359..>33030)
                     /locus_tag="P5335_01535"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HRZ73590.1"
                     /translation="WLDKVYSKEEGYAHRNGDVHIHDLDCLTGYCAGWSLRVLLNDGF
                     NGVRGRVESKPPSHFREALGQMANFLGILQSEWAGAQAFSSFDTYLAPYVFKDNLNFE
                     DVLKAVRSFVYNLNVPARWGQSPFTNITLDWVVPADLKEQIPTSNNEHLFQHKITADL
                     VQRAELRGVASVTELRYEHFQPEMNLINKAYYTVMTEGDANGQPFTFPIPTVNITEDF
                     DWYGENTDILFENTAKIGSSYFQNFIGSQYTLDENGQKVENQNAYKPNAVRSMCCRLQ
                     LDLRELLKRGNGLFGSAEMTGSIGVVTINMARLGYLNADNMPMLYQQLNRLLEISKST
                     LEKKRIFIQEMYDRGLYPYTKRYLPHFRNHFSTIGVNGMNEMILNFTKGKHDITSSFG
                     TEMATELLEFIRNRMKEFQEQTGNLYNLEATPAEGTTYRFAKEDKKRFDDIIQAGEGE
                     NIYYTNSSQIPVDFTEDPFEALLMQDDLQCKYTGGTVLHLYMNEKISTPEACRNFVKK
                     VITNFKLPYITVTPVFSVCTIHGYLNGEHEYCPTCDEILIEKLNTEKI"
BASE COUNT        11310 a         5502 c         5245 g        10973 t
ORIGIN      
        1 catcaaatta gatttgttga atgcacgtaa aaatgaaatt tctgcaaagc aatttgtaaa
       61 agaattcaag caaattactg aaaaatcatg tctatgtgtt ggtttagcca attcaagtta
      121 tttagaaatg ggcataccta tcaaaggtca gtcgcaaggt gttgtggttt gtcccggacc
      181 taacttagcg tattttaaca aagaagtttc cctttttaat atggtgcaac atatttatgg
      241 aaaaacaagc gacttggttc ctacaaaccg tccacacttt ttcatcaaag aattgtctct
      301 ttatatagat tactttaaaa aagaaattga tgtatttgaa gaagcaactt cgcaacaact
      361 aaaaaaagta aatcaattta agtccaacct attagaaggc attacttact ataaaatact
      421 atttgaaaaa tcacactatt ttacagattt aaaaagtaaa attttccatg aactgaatca
      481 gtttgaatta gagttaaata aggaaaaaga agtacttgtt tagtttattg ttttaaaaaa
      541 attatcgggt ttaatactaa caacaatact tctaaattgg tatggagttt attctattgc
      601 attaaaatta cacttttagt cataaaaaat tttaaataaa tacaccatta aggttgaaat
      661 tccttttttt aaagagcata taaatttaat taacttactc tatatctaaa ttatagataa
      721 ctaatgttta attaaaaaat ttactttttt aattttataa attcattaaa aatataatag
      781 tgaaatcatt tttataaaca tgatttttat cataataaag gacattttta tcttttattt
      841 ttgtgatcta aatgacaact ttctaattat aaattaaaaa tgaaaaaata ttttgtaaaa
      901 atgatgtttt taagtatttc tatactactt atttattcat gtaaaaatga aaaaacaaat
      961 ctggattatt ccagtataaa agtgtctggt gaaataattg cagaattaac agcaccccct
     1021 tttgtaccaa aaccagttgg aaatagacct gctcaaaaat taattgtaaa catggaaatt
     1081 aaagaaatcg aaggtgaaat ggctgacggt gttaagtatg tttattggac atttggtgga
     1141 agtgtacctg gtagttttat tagaacaaga gttggtgatg aagtagaatt tactttaaaa
     1201 aatcacccag acaacaaatt gcctcacaac atcgatttac acgcagttac aggacctggt
     1261 ggtggagcag cttcttcatt agtggcacct ggacatgaaa aaacattcaa ttttaaatgt
     1321 attaatccag gattatacgt gtaccactgt gctacagctc ctgtagggat gcacattgcc
     1381 aacggaatgt atggtttaat attagtagaa ccagaaggtg gtttacctcc agtagataaa
     1441 gaatactacg taatgcaagg tgatttttac acaaaaggta aatatggaga accaggaatg
     1501 caaccttttg acatgacaaa agctgtggat gaacatgcag actatgttgt atttaatggt
     1561 aaagtaggag ctttaacagg tgaaggggct ttaacagcta aagttggaga aacagttaga
     1621 atttacatgg gtaatggtgg tccaaactta gtttcatcct tccacgttat tggagaaatt
     1681 tttgacaaag tacatattga aggtggagat gcgattaaca aaaacgtaca aactacttta
     1741 attcctgcag gaggttctgc tattgtagag ttcaaagtag atgtaccagg aactttcatt
     1801 ttggtagacc actctatttt cagagcattt aataaaggag ctttaggaat gttaaaagta
     1861 gaaggtgctg aaaatgctaa aatctactca ggtactactc aagaaggaat ttatcatcct
     1921 gaaggtggaa caattcaaaa catgccaaaa actggtaatg gtaaagaagt tgtagttaac
     1981 aaaacgttag accaacaatt gacagacggt aaaaacattt attcaagaac ttgttttgct
     2041 tgtcaccaat cagaaggtca aggtattcca gcagcgttcc ctccattatc taaatcagat
     2101 ttcttaaatg ctgatccaaa cagagcaatt aatgcagtac ttcacggatt aagcggtgaa
     2161 atcacagtaa atggtaaaaa atataactcg ataatgacca gtcaaaatct taccgaccaa
     2221 gaaatttcag atgttctatc ctatgtattc aatagttggg gtaataataa aaccagaata
     2281 actccagaaa tggtaaaaaa agaaagagct aaaccagctc caaaggcagt agatatgcac
     2341 tagtaattaa taaatattaa taaaaaaatg aaaaaatttt tcttaagtgt tgcggcactc
     2401 ataatcacag gtagtttaat aagttttaaa atccccacca caaaaatgag taatattgtt
     2461 actattcaag atagtaacaa aggaaaggct atttacagca aaacttgtgt tgcttgtcat
     2521 caggcaaacg gacaaggaat tccgaatgct tttccgccat tggctaaatc agattatctt
     2581 aataaagatg taaacagagc cattaaaata gtattaaacg gtttaaacgg acctattaca
     2641 gtaaatggtg ctaagtataa tagtgccatg acaagtcaag gactaactga ccaacaagtg
     2701 gctgatgtcc tgacttatgt ttacagtagc tggggtaata acaactcgaa agttactccc
     2761 gccatggtca aagctcagag aggaaaaaaa taagttaaaa caataaatta actgaagaat
     2821 ggaagcacaa tttattaaac gatttcacta ctgcctgatt ttattatttt gggtttccat
     2881 ttctttttct cagctaccaa aaaacatggt aaccattggt gagggaaatt atattccact
     2941 ttatggtacg tctgataaaa aacctgtaac tataaaatcc ttttatttag acatataccc
     3001 tgttactaat caagaatatc ttatattttt aaagacaaat tcagattata gaaaatctac
     3061 aatcaaacgt ctttttgcga acacgactta cttatcagaa tggaaaagtg atttgaattt
     3121 tggggaatta aatccgagtg caccggttac aaatatatct tggtttgctg ctaaaaaata
     3181 ttgcgactgc caaggaaaaa gattagcttc tttggatgaa tgggaatatg tagctatggc
     3241 cgatgaaaaa agaaaagatg ctagatctag agaagaattc aataaatata tattgagttg
     3301 gtacgaaaaa aacaaaacct ataacatttc agttggcaaa acattcaaaa attactgggg
     3361 agtatatgat atgcatggat tggtttggga atggacattt gatttcaata gcatcttttt
     3421 atctggagaa tcaagaaaag acaaagatac tgataaagat ttattctgtg gaagtggctc
     3481 agtaaacgcg acggatttaa tgaattacgc agcttttatg cgatatgcct tcagagggag
     3541 tataaaagca aattatacta caaaaaattt gggcttcaga tgtgcaaaaa acatttctaa
     3601 ttaagtattc aataaaaaat aaattatgaa aaagataatc ttttcgctaa ttacttcact
     3661 attaatattg ggatgcaatt caaaagaaga aaataaagaa ataaaagata aacctataac
     3721 cgatttatca atttataatc tacctgaaaa atggaccaat caagatggag aagatattga
     3781 actgaaagaa ttgagaggta aagtgctcgt tatggttatg atttacacgt cttgtaaagc
     3841 tgcttgccca agattagtgg ccgatatgcg acatattgaa gagcaaattc caaaagaata
     3901 caaagataac gttcaattag tattagttag tatcgaccca aaaattgata ctccagaacg
     3961 attaaaagaa ttttcaattg aaaataaaat gattggaaat caatgggttt tcttgcgttc
     4021 aaatgaagaa aatactagag aatttgcagc ggttttagct gtcaattata agaaaatatc
     4081 tccgattgat ttctcacatt caaatatcat cagtgtattt aatgccgaag gcgaattgtc
     4141 gtttcaacaa gagggattag gagtaagtta caacaaaacg gtttctaaaa ttatagaaga
     4201 agcgactaaa ttagaatagt tacttcaaaa taaaaaactc aaaaataaca tgaaacctta
     4261 caatcaattt tttgttgtaa ttgcatttgc ttttacacca ataagttcat tttcacaaga
     4321 attagaagtt aatcttcaaa taagacctcg atatgaatac cgtaatggat tcaaagaatt
     4381 aatgaaagac gagttttatc caacttcatt tgtatcccaa cgatcccgaa tgagtttttt
     4441 cttcaaacat gaaaaattca aagcgaattt gtcttttcaa aatataagag tttggggtga
     4501 tgtaactacc actagttcat cagataaaaa cggtatagct atttttgaat catgggtttc
     4561 gtattatttt aatgacaact ggtcaacaaa atttggtcgg caagtgcttt cctacgataa
     4621 tcaaagaatt atgggtggaa tcgattggtt gcaacaaggt caaagtcatg atgctgtttt
     4681 ggtaactaat aaaaagaata attaccaatt agattttgga ttagcgttaa ataacaacaa
     4741 cgaaacttta tatgaatcag catatgtaac taattataaa aacatgcaat ttatttggtt
     4801 caaccaaaaa ttttctaaac ttaatttgag cattttagct ttgaataacg gttatcaata
     4861 tgaaaattta gatgaaaata aattcaaaat agcttactta caaacctttg gaacctatgg
     4921 caaatgggat acttctaaat tctatggcga ttttggacta tatgctcaaa ctggcgatct
     4981 tgctataatt aatgataaag ttgatgttgc tgcttattat gcaggattta atttaggata
     5041 taaattcaca acttcgttta aaattgaatt aggttttgaa tatttatcag gtaaagatca
     5101 aaatgatagt gatacaaaag taaaatcgtt ttcaccttta ttcggtacta atcacgcatt
     5161 caatggttta atggattatt tctatgtggg aaatcataaa aacactgttg gtttacaaga
     5221 tgtttattca aaattcttct ttgaaaaaaa taaatggcaa ataacattag ctccacattt
     5281 attttatagt tctgctgata tttataattc agacacttta gaaatacaag aaaattattt
     5341 aggaactgaa atcgatataa ctggttctta taaaatggat aaaaactttt tgatctccgg
     5401 tggcatatct aaaatgtttg cctctacttc tatggaaatt ttaaaaggag gaaattcatc
     5461 caattgcaat aattgggctt gggtaatggt atcatttacc ccacaattgt ttacttataa
     5521 aaaataaatc aatgaattta atttgttcat tttaaaaaat aatgtaatcg tcaattcaat
     5581 atactataaa aaaatcaaaa aaaaaacaag gattcaataa atcaaatgaa agcaaatcaa
     5641 acagtaatat ttctaactat tttaaattta aaataaatta ttagttgtga tttgaatcat
     5701 aattatttaa cttttttggt ctttattttg ctttataaat aaaagataaa aacatcttta
     5761 tttataaatt aactcctaac aaatatctaa aatatgttat caaaatcaca agcaagaaca
     5821 ttttttttag gtggaacggt agtaaccttt ttggtcttta ttgggttaac tatttattca
     5881 ttcatgccta aaaacgatca aacgtatcac gaaaaaattg atgcaaaagt tattcgcggc
     5941 aaagaaattt gggaatcgaa taattgtatg ggatgtcata ccatacttgg agaaggtgct
     6001 tattatgctc cggaactcac caaagtagtt gaaagaagag gagaaggcta tataaaagct
     6061 gttttacaat cacctgttcc atggggtcca aaaggaagaa aaatggtaaa atatgaaatg
     6121 aatgatgccg atgcagaagc gatgattgcc tatttcaaat ggattggtaa cattgattta
     6181 aatggctttg accgagtggt ttccccttta gcaaaagaca aataatcaat aaataaatat
     6241 ataaatgaaa tataaatcac agaaagtagc atattggttt tttgctttat gcatgttatt
     6301 atttgcattg caaattacct acggttttat tatgggcttt gcccgcatag gaatggatgg
     6361 attacacgat tttatccctt tcaatacagc acgtgctgtt cataccaatt tattagtagt
     6421 ttggttatta accggtttca tgggagccgc ttactatatt attcctgaag aagcacaacg
     6481 agagttaatt aatgtaaaat tagcttatgt acagctaatt tccttagcga tagttggtgt
     6541 tttagctatt attggttatc attttaatat ctgggaaggt agaaaatttt tggaaatccc
     6601 gagagaatta gactatttag ttgtaataaa tgtgttgttg tttttaggct tgatattagg
     6661 gactttatat aaagctgaaa gaagaacaac aacagcctta gtgctttcaa tgggattact
     6721 ttttgcagca ttattatatc ttccgggaat gatatggttt gatagtcaag taactgattc
     6781 atttttccgc tggtgggtag tacatttgtg ggttgaaggt gtatgggaac taattatggg
     6841 cggtatttta tcgtacttat taatcaaact tacaggagtt gatagagaag ttatcgagaa
     6901 atggttgtat gtaattgtag gtttaacttt cctttcagga ttgttaggaa caggtcacca
     6961 ttattactac attggagtaa ataaaatttg gttaatcgta gggggaattt tctcagcatt
     7021 agaaccacta gcattcctag cgatggcttt atttgctgta tatatgtacc gaaaaggtga
     7081 aaaatctcac ccgaacaaaa tagcattgtt ctggactatc ggagcttcta ttgtatcttt
     7141 tgtgggtgcc ggtttgttag gatttgctca tacgttacca caaacaaata tttacacaca
     7201 tggaacatta gtaaccgcaa tgcatggtca ttacgcgttc tggggtgctt atgccatgat
     7261 tgtactggca attatcagtt atgcattgcc aaatatgacc ggaagaaaac gttacaataa
     7321 tacaattggt cacatggctt tttggttatc gaatatagga atgttaggta tgaccgttgc
     7381 ttttggagtt gcgggtgtag ctcaagtata tttagaacgc aaaatgaaaa tggatttcat
     7441 ggatgttcaa aacgaaatta gcattcactt tgtagtatta ctactctgtg cgactttatt
     7501 tactgttggt atttcaatgt acatctatga atttatcaaa tatggtaaac caacggatga
     7561 agcacttgta aaagaataat tattaatttt tagttactat tttaaattaa taaccaaccg
     7621 aaatcaattg cccagagttg tcgctgccgg catttggttt cggtttttca aattaaattg
     7681 ttatgataga agtagaaaaa accatctttt ataaagcagt aggttcagaa attgaaattt
     7741 tcaatcacat caaccaattg caattacctt tattgttaaa aggtccaaca ggttcaggta
     7801 agtcgcgttt tatcgaatat atggctaatg aactaaataa aacattagtc accgtaagtt
     7861 gtcatgaaga aacttctgca actgatttaa ttggacgttt tatcatcaaa ggagccgaaa
     7921 ccatttggat tgacggacct ttatcgattg ctgtaaaaaa cggatttatt ttatatttag
     7981 atgaagttgc cgaagcacgt ccggatgtca tcgtagccat tcattcctta accgatcaca
     8041 gaagagaact ttttattgat aaattaggtg aaaccatcaa agcacatcct gattttcttt
     8101 tagtagcttc gtttaatccg ggctatcaaa aaggttttaa agagttaaaa ccctctactc
     8161 gtcagcgttt cgtagctctc acattcgatt atcctaatcc caaaattgaa tctgaaattt
     8221 taatcaatga aactggaatc caagcggata ttgctcaaaa gttagtcgct atcggaacca
     8281 aaatcagaaa tcttaccgaa ttaggtttaa ttgaaactgt ctctacccga ttattagttg
     8341 acgctgccaa actaatcaaa aatggactac ctaaacgatt agccgttcac atagccgttg
     8401 ttgaaccatt aacagacgag catgaaacca ttcaagcctt aaacgacttg tgtgacttaa
     8461 tgatttaaat gaattaaaaa atgggctttg aattagatga aattatttat aaaaaagtac
     8521 tcaaatactt taaaaataag agactgaatg atgttgaact tctgagccgt caaatcaatt
     8581 tatctgatat aaaaccaaga ttaactcttt ttgcaagagc catttctggt gctccaatcg
     8641 aaatttttcc ggctgaacgc gagggcggct ataaaaacaa aagctttttc ctccctattt
     8701 cttctaccct atttccaaca aaacaagaaa atctaaaatt ctattttttc agaatcgtgt
     8761 atttgagtgt tcaaaaacaa ttgaatttga attgggaaaa ccaagaacaa acacatcaac
     8821 tttcattaga aaaagcagtt gaaaccgcac cattggtttt agctaaaatg tttcaggatt
     8881 ttccatccat gcaagagttt tattatgatg cggtgccaaa attaccggtt aataaaaaag
     8941 accaaaccat tgattattct tggttgtacg gaaaatggat gaaaaacaca ctcgaaattg
     9001 aagacgaaaa tgtgttgaaa aactttgatg atgccactaa aaaattacaa aatacggtcg
     9061 ttgctgaaac cacacttcat gcgaaagcag ttgaggaaat cgagtcactt acaattgata
     9121 aaaaacaaca agaagattac gtattaatgc acagtttcga gaaaattgaa acagcagagg
     9181 aattcaacgg caattggcga gattttgatg gtgccgatga tttaaaacaa catcaagaag
     9241 cgttggagga gatgaatatg aagtttaccg ttcgtgtaga cgatatggtg cattcggtct
     9301 atcaagcgga ttttgttgaa aacacttcta tagccgaaag tgccgaggta gatgaaaaag
     9361 ggttccattt aaaatatgac gagtgggatt ttaaaaagcg aaattactta acgaactttt
     9421 gtaaagtata tccaaaaacc caactcaaaa cccacgcaac gtattataaa aaaacgatgg
     9481 caaaatataa aaccacttta aatggtttac gaaaaatgct aacaagtgtg aacaataaaa
     9541 tgcaacaaca acgaagacaa ttacaaggag acaccttcga tttagacgcc ttaaccgatt
     9601 tatatacaga cattcattcc ggaaaatcac cggatgaacg catttattta tctcaaagaa
     9661 agaaaaacaa agacattgcc attttggttt tgttagacat tagtctttca agtgatggtt
     9721 atgctgctgg aaatcgtgtg attgatgtag aaaaagaagt ttccatctta tttggcgaaa
     9781 ttctgcatga gttcgacatc gattttgcca ttgacggatt ttattctaaa accagaaatt
     9841 ttacaactta tctcacacta aaagatttca atgaaagttg gaacaaagcc aaacataaaa
     9901 tcggagcggt tgaacccaat ggttatactc gaattggtcc agctttgcga cacgccggag
     9961 cacgaatgga tcaattggat gccaaaaaca aatggattat tgttctttcc gatggaaaac
    10021 caaacgatta cgataaatac gaaggacaac atggtattca agacatcaaa caagcattgc
    10081 gagaactaaa cgaacgcaaa attaattcgt atgctttagc catcgaagca caagctaaat
    10141 attatttacc acaaatgttc ggtcaaaatc attatcaaat attaacttct cctgaggctc
    10201 tactaaaatc attggtaaaa ctttatgaga aaatcaaaca tcaataaaat gtcagtatca
    10261 gaaactccta ccatagtaat agaaggacat cccgtaaata cttatttctt agaaagcgaa
    10321 ttaattcatc agttattgga agaaataaaa gcgattaatc caaaagaaga ttttcagaaa
    10381 ttttacaatg ttttcaatca tttagctatg attgaaaagc gttttgaacg aaaagaaaat
    10441 caattgtttc catttttaga gcaaaaaggt tggacaggac cttctcgaaa tatgtggtct
    10501 tttcacgata caattagaga gatgtttcgt attgtcagaa aaaatttgga agaacaagat
    10561 tttacttctg ccaagcataa taccaattta ataactcaaa atttatatcg cttattagat
    10621 gtcgaagaaa acgtgttgtt tccaaatgct ttagaattat tatctgaaga agattggata
    10681 aaaatgcgaa atggagaagt cgaaattgga tggatgttag ccgaagcacc ggcaaaatat
    10741 ccaaaagaat cagaatatat tcatccttca catgatacag aaagaagaac cgacgtaatt
    10801 tttgatgaaa aagcagctca ttatgacgag ggttatatga cgatagaaca agtgaatctg
    10861 ttattcaaaa cactcccaat cgatattacc tttgttgatg aaaacgataa agtaattttc
    10921 tacaaccgag gagatgaaag agtttttcca agaagtgcag gaattattgg tagagaagtt
    10981 cgtttttgtc atcctccgaa aagtgtcgat acggttttga aaattctaga agcctttaaa
    11041 aaaggcgaac aaaatgaagc ttccttttgg ataaattaca aagaacaact tgtttatatt
    11101 cgctatttcg cagttcgtga ttcacaaaaa aattacaaag gagtgattga aatgtcacaa
    11161 gatattactg atatcaaaca aattaatggc gaaaaacgat tattagaatg gaattaaaag
    11221 aaaaatcaat ctattatccg ccgggaggaa ttttaatttg gattataatt tatttggaac
    11281 tcattacctt tggaatggct attattgcct tagcatatta tggttcagag gaacgcgatt
    11341 tatttcacaa caatagttta aaactgaata aaacgattgg aactattaat acgattcttc
    11401 ttttaacaag tggttttttt gttgccaaag gaattcaata ttttaaagta caacatatta
    11461 aaaaaacgtt acttttcttt aattgggcta ttgttggagg ttttggtttt ttagttttaa
    11521 agtcgattga atattatgaa aaattgaaag ttggtctaac gatggattat aattcgtttt
    11581 tcacctttta ttggttatta acaggttttc acttaataca tgttatagtt ggactggtac
    11641 tattaattgt aattagtttt agcattagaa aaaaacaaat cgaagcatca tttgaagaca
    11701 ttgaagctag tgcaagtttt tggcatatgt gcgatttaat ttggttactt ctatttcctg
    11761 ttctttattt acttttctaa tgtatacaat atgaaacaga ccctatcttc gaattatata
    11821 ttgttactta tcctcactgt agtggctgct ttagttgcat ttaacgtaac tcaaaacatt
    11881 atggtaaaaa ctgttgttgg tctagcaata ttaaaatttt ggctagtagc atttcagttt
    11941 atggagttaa aaaaagccca tcctttttgg aaatttttaa ttatcctctt tggaattatt
    12001 tttggattaa ttataatgat aatcctttag ttagtaaagt tttttttgat ctgatctgat
    12061 ttgctttaaa caattcatta gcatgataaa tgactattta ttagcttaaa aaaaaattat
    12121 tacttttttt cattcttttt ataaacaaat ttaccaggtg actattaaat actattatga
    12181 atagattgca agtccaattt ttgttaatca ctccaaattt attattggat ataaaaaaat
    12241 tgataccttt ttatttataa aaataaatta tagtgccaaa tcagtttttt tttgtcttca
    12301 aaaaagtttc acctgatttt aaacccaatg tcaattcttc caaattagta ctttctaaca
    12361 tttgctgcaa attgttccga atggatttaa atttttcatg taaaggacat gggtgctttt
    12421 cgttacatct tgccattcct aatccgcaac ctttgaaaac agcatcaccg tcaatggcct
    12481 gaacaatatg agctaatttt acttttgcaa ttaaattttt aggaatgtaa aaacctccat
    12541 tcactcctct taccgataga ataacgccat ctttggttaa cttttgaaga attttagcgg
    12601 taaatgcttc aggggaatca atcattttag caatgtcaac aaaaccgact ttcttatcca
    12661 ctaaagaatt attagcaata aaaattgcag cttttatggc atattcacac gattttgaaa
    12721 acactattaa ttttttatgc aatattataa ttataaattt atttacaaaa ttatattcta
    12781 aatttaaata gaaataatta ttgaaaataa ttgaaataat cctctaaata gtttttaaga
    12841 ttagttctat attcattgct atcaccatct gaaagactat ttatttttgt ctttaataaa
    12901 attaaataat tatgtaattg ctcatgagct tcccccgtca tagtacattt tttaaaaatc
    12961 atctcaaatt cagcttttaa tcctgatatt aattcttcag gattaccatt ctctttcgaa
    13021 taactatcca ttaatagttg catattttta ataccttggg tagtttcggt atttgcttcc
    13081 cataattggc catcatttaa ggaaacagcc ggaaactgaa aatcagcatc atgttcaact
    13141 tctgtttctt caacaattac tttttgttgt tcgttttttt tacaagaata aaatccaaca
    13201 atcaataata aaatcatcag tctaataaaa gttttcatag taaatagtaa taaattaaaa
    13261 aatgactgta taaaaagcag tcatttttcg gttaatagag ttagtaaatg atttttattg
    13321 aaattgctct tgcaatgcaa tagctttcgg aaacaaaatg ttattttcta aatgaatgtg
    13381 tttatgcaaa tccaattcaa attcttgtaa cattgaaaaa gtaactttat aggtgttaca
    13441 agcatcttca ggagttgtat aattattcga caattctgca atttttgcat aaattactcc
    13501 ttcactttca tgttcttgtt tcatcattgc aattggattt tcaacagaac caaaatgtga
    13561 ttgttgaatt ggttgatttt ccttttgtgc tataaccatc tttttgatat aaggaaaaag
    13621 aatcaattct tctttcttca tgtgttgagc caaagcacct gcactcaatt taaacagaga
    13681 atcaatttca aaaagttccg gatgacgatc gccatgcact ttgcataatt tatctaaaaa
    13741 ttgaagcaat actggtgatt tatcctcaac ataagaatga tgtttttcaa taatataatc
    13801 gattagtaat gttgatgacc acgatttaaa gtcaattgca ttatcatttt tagtttctaa
    13861 aacggtctgg atttcccttt ctaaatcagt aggattaaga ttttttttat cacaaacttc
    13921 ctctaatgtt cggttacctt tgcaacaaaa atcgattcca tattttgaaa atattgcagc
    13981 tgttctaaaa tcattggcaa catattcacc aacggttaat tgttctactg tattcattgg
    14041 aagtaatttt ttttaattaa aaatcaaaga taacgtgatt gcattttttt atttatgacc
    14101 tcaatcatag aagacttcta tttataaaat tttaattact gaattgtttt atacataatt
    14161 acatctgaaa tactttttgc tctcaatttt atttcttccg ctttttcacc tttaaaaagt
    14221 tcgtcaacgg tttgtctaaa cacttcattc caatgatcaa aatgagcatg tgaaagggga
    14281 catttattgt gcaattcttt gtgtttttgc atcggatttc catcaaagtt gccggtaaaa
    14341 aacaagacgt tttcccaaaa atcatacatt ttaggtaaat gattttccca attgacatga
    14401 gcaacttcat tgaatataaa acctattact tgatcagttt ttattttatc gtaaaaagca
    14461 tccactaatc ggataatgtc atttcggttg tgtatatctt tcttcatctt actttaaatt
    14521 aggttattac aaatcttttc ggatgaattt tcgcaacgag aaataaaacg gaattactac
    14581 ccaaagtatc aataatccga aggacgcaaa aaggccggcc gttgttccga aaaaatcttt
    14641 aaaaatagct ccggtataac ccatcatagc tgaaacatct aaatgcaata atatttgaat
    14701 tcgggctaaa tctatcggac ttaatgctgt tactgctacc atagcttttt caataggata
    14761 ttcggacaat tgaaacaata aaaacaatac taatccatca aaaagtaatg caaaatacaa
    14821 ccatgttaag atggcaaatc caatcccttt tgctttgtca cgcgttagaa tggaacataa
    14881 aaaagcaaga gctataaaca ctaccgaaat caaacatccg attaaaatca tcatcaatcc
    14941 aacagcattg ggtgcattga ttaacaacgg aatacccgct cctataaaaa aagccaaaac
    15001 catcgaggca ctcaaaccaa aaaataaact ttgccagatt ttgtttcgtt tgataggctg
    15061 actcaataat aattcgataa actcggaact gttatataaa tagatggtgg tgaataaaat
    15121 agacacgagt ggaaccgtaa ataaaatgac atttaatatc gttaaaagtc ccttggcact
    15181 attgtcttct aaaccaaaag cactccagga caacagactt aaaacaatag tatagactaa
    15241 aacaattttg ttttttaaga tatcggttaa tatgattttg atgattctgt tcattttttc
    15301 tgttttagaa tacttgcaat agcttttgaa atttttgttt cattggtttc cgttttcaaa
    15361 tcgtttactt ttttatgaaa atgaaccttt ccttcttgca taaaaataat ttcggttacc
    15421 aaatcatcta attcactcaa taaatgcgac gtaattaaaa tcaattttcc tttttctttt
    15481 tctttaatga ttttatcctt caaaatttca gaagctaacg gatctaaacc ggctgtaggt
    15541 tcgtcaagaa tcaacacctc aggattgaat aaaaaagcca acgtggcact tactttctgg
    15601 gtggttcctc cggaaagcgt tcgcatttgc ttatccgcca ttttatctaa ctcgaaactg
    15661 tgccataatt cttcatccaa ttcctgattt gaatttcgaa tcgctttaat catttccacc
    15721 acaccaccaa tagtcatcgt atcaggatat cttccaattt gaggcatata accaatattc
    15781 gaacgatata aatactcatt gacaatgctt tttccgttaa aattaatgaa cccttcgtcg
    15841 gttaaaacca ttcctaaaat tgattttatt aaagtggttt ttccacatcc atttggacca
    15901 atcaacgcaa tacattcgcc ctttttacag gaaagcgaaa catcatgaag cacgtttaac
    15961 tttccgaatt ttttagagat atttttaatt tctatcataa ctgcaaaggc ttcatgagtg
    16021 gtgaatggtc tataaaatta tcaggggtaa tactgggcaa cactttttcg gatttatcta
    16081 acaacgtaat cataaaactt cgaaacagta acatggcaga gggatttttt tctaccaaaa
    16141 ctgcaaataa actgagcgga tgataaggaa catctcctat cccgtctcga tttaaatcat
    16201 acccttcata tttgtcccaa tagttgttgt taaatgaatt taatactaaa ctcccatttg
    16261 ttcctatgtc aaaagtgttt cctttaaagt tattgtttac aactacatta tccatgctac
    16321 tagcttgaat ttgcatggcc caaccattag cattaaattg gtttttttct actaaatttc
    16381 ggctactgcc ttctacataa atagctgaag tgtttgcatt aaatttatta ttaaaaatat
    16441 agctgtcggt aatatctttt aaaagtaacc cgtatgctga agctccccaa ttttcatcaa
    16501 atgtattatt aaacattttt acatcttttg agtacattac tgctacacca gctccattat
    16561 ttctaaacaa atttgtgata taggtatcac tatgcgaaaa cataaaatgc aatccatagc
    16621 gaacattatt tgtactaatg tttctccaaa tcaaagaatc gtaaacaaat tcaaaataaa
    16681 ttccatctct gtgtccatca attttattgg caataattaa aatattattt gatttccaac
    16741 aatgaattcc gttacccaat ttttgttcct cttttccgta cgctttgatg taattatttt
    16801 tgatggtagt attttgacag ttttgaagat agataccaaa aaaattatca tctaaaacgt
    16861 tattttgaat taaaacatga cttctattat atactttaat accacacgga tcggttatag
    16921 tagcataaga acacttaatt attttgaatc cgtcaactac aacactgtcg gcatgtatag
    16981 ataaaacttc atgttttttt tcaccatcta aaaccggtaa atttttgcct aaaaaaacaa
    17041 tttttttatc aattattata tttccttctc tataccatcc tccatgaact attatagtat
    17101 cgccatcttt tgctaaagca attccctttt taatagattg aataggatga ttttttccta
    17161 tatggattgt agtcgctttg agcgatagag atataaaaaa taatatgaat aggaaaaatt
    17221 tcatttgttt ttatttaatt atttcgttcc atgaagtaag atttgcttta aattctgttg
    17281 ccatattttt agcttcatct tccgttttta ctgcaataat atttccatgc attggactat
    17341 taatttcgcc cccttgtgca taaaatgcag tttcagcagg aattaaaaca ttcatttggt
    17401 tgaaatcatg tatataaaaa gattgaactt ttacagaaga attttcttta tgataattaa
    17461 ccatgcaatg catgtcgtca aatttgtata ttcttccttt agcggtaatt aattctgctc
    17521 caaatttccc atcagcaatt ttcattttac agtattcgca accatcaata tttaatttta
    17581 tagggtccgc ttcctttgta gcacaagaaa cagtaagcat catgattaaa aaaaagtaaa
    17641 ctgatttcat ctatatcgct tttttaaatt taaattcttt caaaacaatt acaaatagta
    17701 gtatacctgc tccaataagc atccatcccc caatatcagg aactgagtag gctccaaaat
    17761 tcaataattg tttgtatcca attagtggag gttgatagga catacccggc actttaatag
    17821 ccgcattagg atcaagatta tgtccgtatt cataattcca tctataaaaa tcgacacctg
    17881 caagaattac aaaaagtatt atggttacaa aaaaaccaaa aacagctctt ctgttattaa
    17941 taaaaacaag tgctaacgaa ataagagcaa aagcaacaaa tacataagga agtacggtaa
    18001 attcgaaaaa gttttcggtg tgtaaagtcg ccatgccaat gtagtggttc aggccattta
    18061 tgatatctac atctcctcct attttgttgg cgtgaagttg taacattaac ccttcagggt
    18121 attgtggagc tgataattca attctccaca tgggtacaaa taaagcacca ataaataaaa
    18181 acccaacgat gactaacaaa acttttgaaa gaagtgttaa attatttttt ttcatgattt
    18241 ctgattttgt aaaaaatctt tatttgttat aattaattca attaagcatt tataaaaaaa
    18301 caaaatgatt ttttattaaa gttttaagcg aagatttttt gttctattgt aaaaaaatta
    18361 gggagcattg ctgctcccta aaaatattat tttgaagtag caggtaaatt tgtacctaaa
    18421 ctataactta aaggaacgtt gcttccggct ggagaaactc ttatgtatcc ctgcatttct
    18481 tggtgtaacg cactacagaa atctgtacaa tagaaaggaa ctatacccac ttttgtaggt
    18541 ttccaattta atgtagaagt ttcacctggc ataataagca gttcggcagt ttgagctctt
    18601 ctaattgaga atccgtgtgg aacatcccaa tcttgttcaa ggttagtaat gtgaaaataa
    18661 acatcatcac ctactttaat tccctcaata ttatcaggag agaagtgaga acgtaccata
    18721 gtcatataaa catgtacttt atttccttct ctgacaactt ttgtttcttt ttcacctttt
    18781 gctacccttg ggtgattatt tttcttaata tcgtaaattt ttaattgtcc gttatttcga
    18841 attaaatcag caggagcagc ttgtgcataa tgaggttcgc caatagttgg aaaatctaac
    18901 aataattgca ttttgtctcc gctaatatca tataattgag cagattgtgt taattcaggc
    18961 ccggttggta aatatctatc tttagtaatt ttattataag caattaaata ttttccaaat
    19021 ggttttttag aatcaccacc gggaatacac aagtgaccta cagaataata cgttggcact
    19081 ctatctaata cttgcaacga tttgatatcc cattttacaa cctcagaaga aacaaagaag
    19141 gtggtataag catttccttt gccatcaaat tcggtatgta aaggtcctaa gcccggtttt
    19201 tgaacttcac catgaagtac ggaatcatat ttcaaaaccg gaataccttc ataatctcct
    19261 tcataatttt tagcagcaat tgctttagtc attttatcaa aactaaatac aggaattaat
    19321 gctgctaatt ttccagaacc tacaatatat tctcctgttg gatccacatc acaaccatga
    19381 ggtgattttg gacaaggcat taaataacaa atgtctttca ttgccattgc atctaaaact
    19441 aagacttcct ccttaattac agaagttccg gtatgcgttt catcattcca tgtattatgg
    19501 gcatattttg ttttttcgat tttaccttta ccggctttaa tatattcttc cgcttttttc
    19561 cagttaactg ccaagataaa atctttatct ctttgggaag catttacttc taaaagcgta
    19621 ttggcttgtt cagaattata acaggtaaag aaaaaccatc catgcgattt tccttttccg
    19681 gcatgtgaaa ggtcgaaatt aactccaggt aaacgtaatt gaaatgccaa gtccatttta
    19741 ccagactctt tatccacttt tacaaaacta atgtgagctt taaagttttc tttgtatgta
    19801 acaataggca cgtctccatc ttcataatcg cccggtaccc caaaacgagt tcccgctacc
    19861 acatattccg tattctcggt aataaatggt gacgagtggt taccagccga atttggtaat
    19921 tcaatgattt cagcagtttt aaaacgtttt aaatctactc tagcgatacg aggtgtattg
    19981 tttgcattgg caaaaatcca tctaccgtcc acttcgccat ttgtttgaga taattctaag
    20041 tgatgttgat catcccaagg aataaagcct tcggaagtat taagcattgg ttttgtttct
    20101 tcgctataac cccatccact ttctgggtcg accgagaaaa taggtacaac tttcaacatt
    20161 cttccacttg gaagcccata cacactcatt tgaccactaa aacctcctga gacaaaatta
    20221 tagaattcgt catatttacc gggtgcaaca tatacttttt gagcagcatc tccactaact
    20281 gtatcacttg tgtttttggg cttacaagac cccatgaatg cagctatcag aactacagca
    20341 gaagtaattt ttaaaaattt tgttttcata tactttcgat ttatattaaa gttgttcatt
    20401 ttaaaaatta tttcacgcca tcgttttgac gcatgtattc taaaacatgt ctggcatcgt
    20461 catctgtaag tgattgatta ggcattctta ccaaacacaa ttccaattgg gcttgtaatt
    20521 caggatcctt gtcaatcatt gggtctggat tagttgcaaa attcataatc cactctggag
    20581 ctctgcgttc ggtcacacct ttccaaccgg gtcctactaa acgttcgtcg gttagtttat
    20641 ggcaagacat acattttacg cccgccactt tctcgccttc tgcagccatt gcaacatcta
    20701 agccggttcc taaatcaacg gtagtgaatt taccttcacc acgttttgga tcataagaag
    20761 caggatctga agaggtttca gaagaagttt ctgttgaaga agactttgag aaatcttctc
    20821 ctgcattggt tttatctttt ccacacgaag tgaataagag taaaccaagt gctaatgcat
    20881 acaatttgtt catattaatt taagttaaaa ttatgacaca aatttcctga gtaattattc
    20941 gaaaatttat gattgcaatc atacaaatca tgtttttttt actattatct ttgccccata
    21001 aattaatagc tcgttttgaa atattttatt atcatagtca ccttcttctt tttattcaga
    21061 ccgatatttc cggtttttaa ttatgtggtg aattatgatt atatctcaaa agaattgtgt
    21121 gaaaacaagg aaaaaccgga actaaaatgt aatggtaaat gccatttaaa agcagattta
    21181 gccaaggcat ctgaagaaaa tattcctgct tcacaagaga caaaaagtaa tataacctta
    21241 gaattattat tcttagaaac ctttcctgaa tttaaattca atcgaattta tgttgaaaaa
    21301 caacaggttt ctatttctta ttccagacta tactttcgat tacatagtac cagcattttt
    21361 catccgccca ctgcttaatc tcccttttag tattttaaaa tttctaaacc ggattaaatt
    21421 ctaatccaat tatatactag tacattattt ttaccataag ccgatataat cggtgaattc
    21481 caactcattg ctttatgggc ttatggttat tatttctata actcaaaatt aaatttacaa
    21541 tgaaaattta taaaatattc ttgctttttg ctcttgctct atttacttca tgttcgtctg
    21601 atgatgattc aacaccggtt aacgaattag acggtttagt aaaagtcaaa gaaattacta
    21661 atgacacgca tgtagtcgaa ttatacacta catctggcac tacacaacag ggctacaacg
    21721 atatcacctt gagaatcaaa gataaaacaa ataacgaata tgtaaaaaat gcttctatta
    21781 catggatgcc tattatgcac atgaccatga tgagtcactc ttgtcccttt tcaatggttg
    21841 aaaaagttgt tggaaaagaa acgttgtaca atggctctat cgtttttcaa atggcacaaa
    21901 atgaaacaga atattgggat ttaaaaattg attattccat taatgaaata gactattcag
    21961 taacagatat cattgatgtt cctgctactg aaaaaagagt ggtttcatcc tttatgggaa
    22021 ctgataacat tcgttatata gtagcattag ttgatccaaa aaacccaaaa gtggctttaa
    22081 atgacatgac agttggtgta tttaaaatgg aaaatatgat gagttttcca aaagtaaatc
    22141 aatacaaatt gaaaattgac ccaagaatgc caagcatggg caatcatggt tcgccaaata
    22201 atgtagactt aacccaatca gcttctactg gtttatacaa tggtaaatta tcactaacca
    22261 tgacaggata ttggaaaatt aatcttcaac ttttaaatag tgagaatacc gttttaaaag
    22321 gtgaagaagt tacggaaaca aacctggaaa gttcacttta ttttgaagtt gaattctaac
    22381 tacagtagta acggccaaag gttatttaaa cttttggcct tttatttctt ctcatgaaaa
    22441 atcaatttta tgttgtattc gttcttttga ctacaacctt gagtttggca caagaacaag
    22501 tgaaagatag cattttgccc gtaaatctaa ctgaagtaat tcttattgga aaacctacgc
    22561 aattaaatga aaaacaagta aaaccattag ctagtttaga tgaatacctt caacaatcgg
    22621 gtagaataga tatgattaaa cgcggaagtt atgcttggga acccatgatt aacaacatgg
    22681 caactgagcg aacgattgtc accattgatg gcatgcggat atttggagct tgtaccgata
    22741 aaatggatcc tgtaacttcg tatgtggaaa tatccaatct gtctgaagct acaattgcgt
    22801 ccggacaaga aggaagttgt cacggaccaa ctattggcgg ttcattggat ttaaaacgac
    22861 ttcgcagcaa tcaagcaaat ttaggttggt caggaagttt gagcagtggt tttgaattca
    22921 acaacaagca acgcatttta ggcggcatgt tacattacag agacagttcg atgtatgctg
    22981 atgctaatct cacctatcga aaagccgaca attactatgc aggaaatgag gaagaaatta
    23041 attattcaca gtttcaaaaa atgaatgctt cgattgtttc cggttatcag tttcaaaata
    23101 aactaattga aggttccctt atttttgacg aagccaatga tgtagggtat ccggctttac
    23161 cgatggatat ttcgttggca cgtgccttca ttacttcttt aaaatacgaa gtggcaccaa
    23221 aatcgggatt gctcaatcac tgggaaaata agttgtatta caatacgatc acacacttta
    23281 tggatgattc taagcggcct gatgttccta tccgaatgga catgccgggt tggagcgata
    23341 cctatggtta ttattcaaag ggtaaagcaa ccaaggagaa gcatcatttg caatttaatg
    23401 tgaatggttt ttacaatcgt tccattgccg aaatgaccat gtatccgaat gacccgaatg
    23461 aaaatgccat gtttatgtat acatggccgg atgttcgtac actttacaac ggcatgtatt
    23521 tagaagatga attggaatta tcttgtcata attctctaaa attaaatggt tcgtttggct
    23581 ttcatcaaaa caaagtagcc aatgaatttg gattggaaag tttacaaata ttttatcccg
    23641 aaatggccga ccaacaaaat agaattttaa aaagtatttc gttgcaatac aaacatcaaa
    23701 tcaaagactg gaatttttca gtgaattccg gatatggcga acgagcacct tctgtaacgg
    23761 aaggttatgc cttttatatt ttcaacagtt ttgatttgtt tgattatatt ggaaatccaa
    23821 atttatctaa cgaaaaatca attgaaggaa gttttgatgt ttggtttaaa aacgaaaaaa
    23881 taaaaaccgg cattaccttt tctgcatttc atctattgga ttatattatt ggaataccgg
    23941 atgaaaatat actacccatg actatcggag ctagtggagt gaaaatttac aatcaactta
    24001 cgtatgccaa tttgatgcat agttcatttc aatttgatta tcgattgact gaatatctca
    24061 catggaataa taaattgcaa tacagttatg gaaaagattc cgaagaacgt aacctaccat
    24121 ttattagtcc gcttcaatac caaagtcaat tgcaatttaa gaagaatcta tttcaaacgg
    24181 aagtaaacgt tcgaggaaat gccacacaaa cgaattatag tccgtattat ggagaaaata
    24241 gtacaccgga ttatgctata ctaaattttt cagcaggata caattttaaa tggacttcac
    24301 agaaattatt ggtcaatgcc ggtgttgaaa atattttcga cacttattat tccactttta
    24361 ccgattggaa taacattcca agaatgggac gaaatgtatt tatcaatctt tcttatgcaa
    24421 tcggaaacta actttttctt tgaaggagta aaaaagtaaa tacaaaacca cacttcctaa
    24481 actagcaaac aaaagcgttc ctgtaatgat gaatccaatc atccagggac gactattttg
    24541 catcatttga ccaaatacac cttctgtgat tcctgcttgc cacaaggctc tttgaatgcc
    24601 cattccggtt aatgttgcaa gaaaaagaaa cagtgtaatt tgtgaaatcc aaaagaccat
    24661 cgacattctt ttcaaagctt ttaaactttc aaaagcaggt ttaataaaat aaaaagctcc
    24721 ggctaaaacc atcatggtat taattccaat cgtagttccc atagcatgaa ccaccgtaat
    24781 ttgtgtaccg tgagtatata aattaaaagc gggaattgac ataaataaag cgatggttaa
    24841 attaatgatt acccaataat cggcagccat taaaaagcga taaggaaaaa aatagtaatg
    24901 tgttctgttc tctttcaatt gttggttcca attgtagaaa atgcgaatta aaataatcca
    24961 ttcagtcata ctcactatat acgaaatatg gtgaatgtaa cggtcagttg gcagaatata
    25021 aatgtgatgt ccccaattga acattaaatt aaacaaacct aaaaaataca tggcaaaggc
    25081 taacttgctt tgacccattt tttcatcacc ggatattttg gtcattaaat agaaagccaa
    25141 tccataaatc atttgattaa cagcccctac aatcgaacca ttaaccttcc attgaattgt
    25201 catgtcggcc atcaagtgtt ctttgaaata gggaaaaatc caaagatagt tctccataaa
    25261 aataaaggaa aagaaaacaa ttcccgtgaa ccacatccat ttataaaccg gccaattttt
    25321 ataagaacct gcatttttta aataaaacag tagaaaaaca aaataggaaa tcagtatggg
    25381 taatgcccaa attggtttaa attcccaata ttctctccct ccaaaatcac cggtaaaata
    25441 gctgtaaaaa attccaacta aagtaatcaa ccacaagaac aaatgtattt ttaaaagatt
    25501 gaatgagatg cgtttgacag tttccttttg gatagccaaa gttacacatg ctagtcctcc
    25561 taaaaccaca ccaaaatatg cacttgatac atgtaacggt cttaaagtga gaaatcccaa
    25621 cattcctgat ttagcatcag ttaaacgata ggcaaaggaa gccaaaaaac caaaaaataa
    25681 tcccaaaaaa agcaatagta atgctaaacg aagtagccaa gttcctgatt tcattctaaa
    25741 ttatctaaag taaatgttcc gtttatttgg gttgaataat gtcgcggatt tgcagtgcct
    25801 gtttcattca ttgcttttaa aaataccagt aagtcttcca tttcacgatt ggttaaatca
    25861 aaagcaggca tctgacttat tccgttacta attattgctt ttaaaaacac atccgaataa
    25921 cccggtttag ctgtaacatt ggttaaatca ggacctaaat aaccccctaa cccatacaat
    25981 tgatgacacg tatgacagtt gtgtttttgc caaacatctt taccagaagc cgattgtttt
    26041 tgataggcta tcttttgatg gtctttttca cgcaaaggtt ttagataaat tgtaaaggaa
    26101 tagattaaaa ataaaacact tagcgtgata aaaatcttta atggggatac tttaattaaa
    26161 ggcatactca gtcaatttat tcgtttctaa atgtaatgct accgatgatt gattaataat
    26221 aatcattgcc gtttgcaaca tgttttctaa ttcacaacaa tttctgcatc ggtaatagta
    26281 atctaaacat tccatcaaat cggtatgaat ttttatcaat tgagttaatg ctttcatttt
    26341 ttcttcttga cttccatgca aaacctcata ggtcattaaa tgtttcactt gatttttgaa
    26401 atttgataca accgttggac aaatgatttc atgagaagct acggttctac tttgtattgt
    26461 agtttcattt ggaatcattt tattgaattc taagatgatt tgtagtgagg agttataaga
    26521 atacaccaaa gcttccaaaa gcgaattaga agctaatcga ttggctccat gtaaacccgt
    26581 gcaagaacat tcgcctaacg catataaatt ttttacatct gtttgaccaa attcattcac
    26641 ggcaatccct ccgcattgat aatgtgcaac gggaacaatt ggaattaaat cagtttcagg
    26701 attatatcct aattccttaa attttgaata aatggtggga aaatgatttt ggaaatcaag
    26761 tacatctaaa tgtcgaatat ccaaacacac aaaatcttct ttggcttgtt tgatttcttt
    26821 ggtaatagcc tctgaaacaa tgtctctggt agccaattcc cctctaacat cataatcaaa
    26881 taaaaatctc tttcctgatg aattgagcaa atatgcacca aaacctctaa cagcctccga
    26941 aatcaaaaac aaattactct tgcctttttc atacaaagca gttggatgaa actgtatgta
    27001 ttgcatatcg gtaatagttg ctccagctcg ataagccatt gcaacaccat ctcccgttgc
    27061 aacatcggaa ttagatgtaa aagcaaaaag ttgtccgcta cctccggtcg ctaacacaat
    27121 tgcatttgca aaaatctgtt gctcttcggg ttgttcatga agcatataag tcatgccaat
    27181 tgcttgtttt tcatcagttc tatcgtcagt aatgacatcc acacaaaaag cgtttgcaat
    27241 tacttctatg ttagtttctg ctagaatttt tttccataaa acagtttgaa tatgccaacc
    27301 cgttttgtct ttaaaatgta ccacacggtt ttgactatgt cctccttcta aagccatgtc
    27361 taaaacacct ttggggtcta catcaaattc agctccccat tgcattaatt cattcaatcg
    27421 ttgcggagcc tgtttgatca ccatttcaac cactttcttg ttacatttgc cttgacccgc
    27481 atttaaagtg tcttgaatat gttgttcaaa actatcggta atttggttgt atacggttgc
    27541 aattcctcct tgagccaatg aagtattggt taattgagga tcacttttag agagaattat
    27601 tatttttata tccggttgtt tttcaatcag atgaagagcg agagtcaaac ctgcaattcc
    27661 ggttccggaa attaaaacat cacatgtttt catactttta aatttgaagc attcgttcaa
    27721 taggtttcag ggctttgatt ataatatctt cttgaagtaa aacttcaggt gattcttcta
    27781 acaagcattt atacaacttc tctaagttat tcattttcat aaatgcacat tcactacaag
    27841 cacatgtgtt atcttctttt gaaggagcag gaatcaaaat tttatctggt acctcttgac
    27901 gcattttgtg taaaattcca ttttcagttg ctacaataaa tttggtttcc ggtgctgttt
    27961 ttacataatt tatcataccc gaagttgagc caatatattg tgctgttttt aaaatatgcg
    28021 tttccgattc cggatgtgca ataattaacg cgtcgggatg ttttttatat aaatcaataa
    28081 gtttttctaa tgaaaatgct tcgtgaacaa tgcaggatcc atcccataaa attatctctc
    28141 tgcccgtttc tttgatcagg tacttgccta agtttttatc gggtgcaaat acaatatttt
    28201 tatctatagg aattgaattg attactttta ttgcattgga ggaagtacac acataatcac
    28261 tcatcgtttt aatttcagcc gaacaattaa tgtaagtcac cacttcatga ttgggatatt
    28321 ttttaagaaa cctacgaaac tcatccggca gacatccatc tgccaaggaa cacccagcgt
    28381 taatatccgg aaccaatact ttcttagttg gatttaaaat ttttgccgtt tctgccataa
    28441 aataaacccc tgcaaaaaca ataatatccg cttttgattg ggaggctttg cgggaaagtt
    28501 ccaaactatc ccctacaaaa tctgctattt cttgaatttc gggttgctga taataatgag
    28561 ctaaaatgac agcatttttc tcttttttca gcttttgtat tttatcgctc catttatcaa
    28621 tcactttcat gtttaatcgg atgtttttgt cttattaaag aataaatttt ttaaatttta
    28681 agatatgtct cgcctatttt tatccctgaa attaaattca ttaaatcagt tgaatctaac
    28741 gcttttttta attgttcccg aataggttta tattggttat gaaaaggaca aggctttatt
    28801 tcagaacaat cctgtaaacc taaactacaa cgattataaa cagcatcacc atcaaatgct
    28861 aaaacaattt gtcccaattt aactcttgac aacatttctt ttgaaacgaa aaatcctcct
    28921 ccattacctt tagatgattc tactaactta tttttagcta atttttgaag aattttagcc
    28981 gtaaacgatt caggagaatc aatcttacgg gcaatttcac gcaaactaac tttatttccc
    29041 aattgagatt ccgatgcaat tattatggta gcccgaatgg catattcaca cgtttttgaa
    29101 aacatatttt tttatcaaag ataaatctat ttctcctttt taaaagaaac agtagccaat
    29161 gaatttaaat gtacttaaat ttactcgcca aaaatattct gattaattaa aaaaaaacat
    29221 gatttaaatc atatagattc aatactaaaa aactgaaatt tgtcttctaa attggatatt
    29281 tatgaattat tggaaaaagc taaccgcagc ggaacggatt gctcgtgtac aacaagcttt
    29341 acatcaaaat gttgattttt ccaaagatgc ttcgttgggc tatccagctt caaaacttga
    29401 tggaaaagta ttttatgatg atgctccttt tttgaaagat gccccggtat tatcaactta
    29461 tgttgcaaat cctaatcaca ttggttgtca cacgaccggt acttcggaaa ctgcttttaa
    29521 aggcacccaa gagatagaac gggaaacatt ggatatttta gcaattgata tttttggttc
    29581 aaagaaaaat gagtttgatg gatatattgc ttctggtgga actgaagcta atattcaagc
    29641 actatggctg tatcgcaatt attttatgaa aacgtttgga gctaatcctt ctgaaattgc
    29701 aattattgcc tcggatgata cacattattc gattgccaaa ggttctaact tattgttaat
    29761 tgattggata aacgtaccag tcggttttga aaacagaaaa attgaagttg attcttttga
    29821 aacaaaatta ttaatatcag tatccaaagg aaaaaaatat tttattgtgg tttgcaacat
    29881 ggggaccact atgtttggct cagttgacaa tcctgagaac tatactaaaa ttttagaaaa
    29941 acacaatctt gcatttaaac tccatattga tgcggcttat ggtggatttg tatatccatt
    30001 tttatcacaa gagacgattt taagttttaa aaatccaaaa gtatcctcta ttacagttga
    30061 tgcacataaa atgttgcaag caccctatgg taccggtatt tttttatgta gaaaaggctt
    30121 aattgaaaat gttttaacca aggaagcaga atatgtcgaa ggaatggatt taacattatc
    30181 aggaagccgt tccggagcaa acgcagtggc agtttggatg attttagcaa cctatggccc
    30241 aaacggttgg tttgaaaaag taaaagtgtt acaaatgaga acagattggt tgtgtcatca
    30301 attatctgat ttgaatattc cctattttag agagccaaat atgaatatag ttactataaa
    30361 atccagcttt attcctgaaa aattagcaca tcaatttgat ttagtaccac aaaaacatac
    30421 tgctgaaaat gaatggtata aaattgtagt aatggagcat gtagaagttg atcatttaag
    30481 caaatttttg gagaaactca aaaagtccat ggtcattcat tcctagatta cctcatttga
    30541 atccaaatat ctgcagttga taaatcagtg aacgctttat gttttgaggc gttcatgttt
    30601 tttatagttt ctactccatt tcgaaaaggt tgtatgaaat agtttccggc ataattattc
    30661 gtttgtaaaa ccgatatcat ttcgtccaaa tcatttttgc ctaaaagctc tgaatgaaca
    30721 gttgttctaa tttcaaaagg aatttttgat tggtttaaaa tagccaaact cattaaaaat
    30781 ttttcgtata aatttgattc ggttataaat ttgaatttct ctttagttga tttaaaatca
    30841 agggctacat aatctatcaa tttttggtca atagcttcac ttaatcggtc tggtttactt
    30901 ccatttgtat ccaccttaat caagaaaccc atttctttta tgagttgcat taatacgatg
    30961 gattctttgt gtaataaaca ttcaccacca ctaaaaacaa cagcatccag taagcctttt
    31021 cgttttttca aaaatgaaat tatttcggga atggaataac ttcctttccc gaaaacaatc
    31081 tccggattat aacaataact gcatttcata ttacaaccgg caaaccagag aatacatgcg
    31141 gttttatcgg gataatctaa taaagtgaag gcggttatgc tatgaacaat ttttttagca
    31201 cttggtttca ttaaaatggg tgcgttgttt atgttctcct tttttaccaa tgttaaaact
    31261 ttccacaggt cgatgatagc ccatcacccg agtataaaca aggcatttag ttcgtaattc
    31321 attgtgttgt tctaaaacaa ttttagtagc tgttgtgttc atattttttc agtatttagt
    31381 ttttctataa ggatttcatc acatgttgga cagtattcat gttcgccgtt taaataacca
    31441 tgaatggtac aaacgctaaa cactggcgtt acggtgatat acggtaattt gaaattagta
    31501 atcacttttt taacaaaatt tcgacaggct tccggggtac taattttttc attcatatat
    31561 aggtgtaaaa ctgttcctcc ggtatatttg cattgcaaat catcttgcat caataatgct
    31621 tcaaatggat cttcggtaaa atctaccgga atttgtgaac tattggtata gtaaatattt
    31681 tcaccttcac ctgcctgaat aatatcatcg aaacgtttct tatcttcctt cgcaaaacgg
    31741 taggtcgttc cctcggccgg tgtagcttct aaattatata aattacctgt ttgctcttgg
    31801 aattctttca ttctatttct aatgaattct aacaattctg tagccatttc tgttccgaat
    31861 gaactggtaa tatcgtgttt gcctttcgtg aaatttaaaa tcatttcatt cattccattc
    31921 actccaatgg ttgaaaaatg attacgaaag tgaggtaaat aacgtttagt ataaggatac
    31981 aatcccctgt catacatttc ctgaataaaa attcttttct tttctaatgt tgattttgaa
    32041 atttccaata accgatttaa ttgttgatac aacattggca tgttatcggc atttaaatac
    32101 cctaatctag ccatattaat agtaaccaca ccaatacttc ctgtcatttc tgcacttcca
    32161 aataatccat ttccgcgttt aagtaattcc ctcaaatcta attgtaaacg acaacacata
    32221 cttctaacgg cgttaggttt ataagcattt tggttttcaa ctttttgtcc attttcatct
    32281 aacgtatatt ggcttccaat aaaattttga aaataggagg aacctatttt agctgtgttt
    32341 tcaaataata tatctgtgtt ttcaccatac caatcaaaat cttctgtgat atttactgtt
    32401 ggtattggaa aagtaaaggg ctgtccattc gcatcgccct cagtcattac ggtataatac
    32461 gctttattaa tgaggttcat ttccggctga aaatgttcat aacgcaattc cgtaaccgat
    32521 gctacacctc tcaattcagc tcgttgtact aaatcagcgg ttattttatg ttgaaataaa
    32581 tgttcattgt tacttgtagg aatttgctct ttcaaatcgg caggaacgac ccaatctaac
    32641 gtaatattcg taaaaggaga ttgaccccaa cgagcaggaa cgttcaaatt ataaacgaaa
    32701 ctgcgaacgg ctttcaatac atcctcaaaa ttcaaattat ctttaaaaac atacggagct
    32761 aaatacgtat caaacgaact aaatgcttgg gcacctgccc attcactctg taaaatacct
    32821 aaaaaattgg ccatttgacc taaagcttcc ctaaaatgtg aaggtggttt actttccaca
    32881 cgaccgcgaa caccgttaaa accatcgttt aataaaacgc gtaaactcca accggcacaa
    32941 tagcccgtca agcaatctaa atcgtgaata tggacgtcac catttcgatg agcatatcct
    33001 tcttctttag agtacacttt atctaaccaa
//
LOCUS       DAOTCW010000009        30267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_762_length_30267_cov_46.952337, whole genome shotgun sequence.
ACCESSION   DAOTCW010000009 DAOTCW010000000
VERSION     DAOTCW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 30267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30267
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_762_length_30267_cov_46.952337"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1255)
                     /locus_tag="P5335_01540"
     CDS             complement(80..1255)
                     /locus_tag="P5335_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRZ73591.1"
                     /translation="MKNIIITFALFTSTLLFSCEKKNNLAETPIRLTETHQPAIATDE
                     TNGKIQVAILLDTSNSMDGLIEQAKSRLWNIVNTLTTLKFEGKTPEIEIALYEYGNDG
                     LNKNTNYIRQVAPLTTDLDLISEKLFSLRTNGGSEYCGAVIQDASQKLTWNDGLQNMK
                     LLYIAGNEPFNQGSINYKEAIADALKKGIYVNTIHCGSYEEGIRGLWKDGADVGQGKY
                     FNIDSNAKVRYIVTPYDDQISQCNVRLNTTYIGYGSKGVEKKMAQEVQDANAQSISGA
                     NYTERAVSKSKTVYKNESWDLVDKAKTNAKALDNLKKEELPAELKNKSKEEIKVIVTQ
                     KTKERETIQKEMEELSKKRQAFIDEESKKTKTQDDLGQAITTSIHSIAKTKGYVVVN"
     gene            complement(1273..2604)
                     /locus_tag="P5335_01545"
     CDS             complement(1273..2604)
                     /locus_tag="P5335_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73592.1"
                     /translation="MKAKIIWGLSALTSCIPLIYVWNTNPKTEPINASPSKELAILET
                     DITEEEEKPCVFMTLMGEQTSEGFAVAKTAEKFQETISKGELWLLEAQNTDGGWGAGL
                     QSNQGERNPHAVSSDPATTAMSAMALYRCGYSMESGKHKETLEKAVHFLLNEIDKNQN
                     LPYITAIRGTQIQGKLGQHIDAILTLQFFNQLLPTLKNEAMKTKVKAAIQICVDKIEK
                     SMDNSGKVGGSGWAGVLQSSFANSSLEMASKNEGIKVDQEKRQKARDYQKSNYNPANA
                     SAKTEDGAGIMLYAVSSSVRGSAEEAKDADKIFKKAKEEGRIEKDAQLNEANLKRIGV
                     SDEKAKSYDVADKVYKSAKVKAMNEDVLTGFGNNGGEEFLSFLQTGESMIVNHDDEWK
                     KWYDNIGGRMMQIQNADGSWSGHHCITSPSFCTATCLMILSVENDIQKLQH"
     gene            2752..4551
                     /locus_tag="P5335_01550"
     CDS             2752..4551
                     /locus_tag="P5335_01550"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRZ73593.1"
                     /translation="MYNQLKILLVVLFFPLMMVAQDSTKTKVSKTSVSKKAKEVEQTL
                     SKSNPESIADKYVELANELDKKGDYAKAEDYLKRALPIYQKQKIKDKIASTTRAIAQI
                     QEKQNKVKDAIKSYQSASEEAVDVGYSKVNSNDASRLQNTNPKAQAEYLDSNIKILEK
                     EGKMDDAAEVYKQKAEVDFKSENKGMAIENYEKAIAVSKDKPEEVLKLKSEISKVYAS
                     ENNLDKAIEITKNTISEAEKLGSISQQIEQQQTLADLYFKNENQDDAIVLLEETYALA
                     LKNGRTFDAKKAMLALSDYYRQQGNQTKAIELYDQFLNDFDRMVQADSTLVDSKIFQV
                     TEEKIRQLEKERALKDELITKKNKFNYVLIGSVILMLILLGLIAKALFFIKTKNKKIA
                     LQSLRREMNPHFIFNSLNSVNQFIAQNNELEANKYLTSYSNLMRNMMETSNKDFISLS
                     NELEQIKKYLDLEHLRFQDKFEYELEVDEQLDVDVILVPNMLIQPHLENAIWHGLRYR
                     ETKGKLIVTFAEENQLIKVSIEDDGIGVEKSRELKTQNQKVHESRGLNNVEERINLLN
                     DLYHQQISYTISSGTDGKGTIVTLYFSKTTKVA"
     gene            4548..5303
                     /locus_tag="P5335_01555"
     CDS             4548..5303
                     /locus_tag="P5335_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRZ73594.1"
                     /translation="MNDFQKIKSVIVEDESAAREALKNYLAKYCPQIEIIGEAQNSRE
                     AIPLLHDLQPQLVFLDVEMPFGNAFDVLEGCKDLSFETIFVTAFSEYSLKALNQSAAY
                     YLLKPISIEELILAVNKVQQQLMKQELFNRNQIIVQNFREPRIEKQQVILPTLEGFEV
                     VKMEEIVRLRGNGNFTDIHLANGSKKMVCRFLKHFDELLDFPFMRVHKSHIINLNYVK
                     SYHKSLGGYVMLFDASEVEISPNYKEEFLRKFN"
     gene            complement(5313..7274)
                     /locus_tag="P5335_01560"
     CDS             complement(5313..7274)
                     /locus_tag="P5335_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRZ73595.1"
                     /translation="MRTKKQKIILSLKLFLVFLVLIFITLYFFRDVLLQKSITRIQTK
                     FETDFNCRFSVKKAAFSGLTEVELVDILLIPKNADTLLSVEKIKTNYSFFQLLTGNIR
                     LNNMEMSNGFVQLIKNKNGRNFDAFLKKNPEEEKTTEKRDYAALANRLLTKVFNLIPA
                     EMTLSNLSLKIDDMGRKVNLHLNKLTLEDHQLQSSIKVKTNSFSQNWQMKGYADPRLK
                     KADLKFSTYDSTKIRMPYIEERFGLFSSFSAIHIVLNNLTMEEGKLYIDGVTEIEDLT
                     VNHPKIASKDVIFEKTQIDYHFILGSNFIAIDRSSSAKLNKITLRPFVKYSTEKDTIY
                     TLKIGVPKMKAQDFVNSLPTGLFAHFEGMQVQGAFDYQLDFEFNKNNPEALIFESKLN
                     KENLKILKYGEADLSKLNGEFVYNAIENGRKQRSVLVGAGNPYFTPLEEISPYLQKAV
                     LTFEDPQFYNHSGFSTDAFKQSIAKNIKTKKFTRGASTISMQLVKNVFLTREKTLSRK
                     LEEILLVYLLENNRLVPKARMLEVYLNVIEWGPNVYGIGESAQFYFEKTPRELTLNES
                     LFLASIVPSPKKFMWLFDKEGNQKPYAVSKQKMYKNIMLSRGLIEAQDSITAAIPVKI
                     KGISKTYLRLKTEKDSTAIDSIEIEEADF"
     gene            complement(7308..8669)
                     /gene="radA"
                     /locus_tag="P5335_01565"
     CDS             complement(7308..8669)
                     /gene="radA"
                     /locus_tag="P5335_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRZ73596.1"
                     /translation="MSKVKTSFFCQNCGSQYAKWQGQCHACQQWNTIVEEVIQKEEKT
                     SWKPSSSEVKKAPKPLRIKDIDSSQEIRLDTTDGELNRVLGGGLVPGSLTLLGGEPGI
                     GKSTLLLQISLKLPFKTLYVSGEESQKQIKMRAERITPNGDNCFILTETKTQNIFKQI
                     QEIEPEIVIIDSIQTLHTDYIDSTAGSISQIRECTAELIKFAKETNVPVLLIGHITKD
                     GTIAGPKILEHMVDTVLQFEGDRNHVYRILRSLKNRFGSTAEIGIYEMQGSGLREVAN
                     PSEILISHKDEELSGTAIASTLEGMRPLMIEIQALVSTAVYGTPQRSTTGYNAKRLNM
                     ILAVLEKRAGFRLGAKDVFLNVTGGISVDDPAIDLAVVAAILSSNEDIPVGKNFCFAG
                     EVGLAGEIRPVNRVEQRILEAEKLGFSTIFVSKYNKISLKNTLIEVKLVAKIEDVVEQ
                     LFG"
     gene            complement(8672..9820)
                     /locus_tag="P5335_01570"
     CDS             complement(8672..9820)
                     /locus_tag="P5335_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRZ73597.1"
                     /translation="MKLIQTLVIVLCSIPLFSQKTIETFTSKKLGADREIYISLPPSY
                     EKDTNRNYPILLVLDAEYLFDPFQGALKFGNYWDDLPEVILVGICQNKTEERYADSEF
                     DEETGLPDSTGANFFDFIGTELMPYLEKTYRIAPFKIIAGHDTTAGFTNFYLYKDNPL
                     FDAYITLSPDLATDMENRIPERLGLITKPIYYYHSTADGDVKKMQKRIKELDEKAKVI
                     KNTTLNYKFDEFIGASHYSLVLHSIPNALYQFFAVYQPITTFEFNEKIVKLEYGYVEY
                     LKTKYDVLEKSLNIKMPIRLNDFKAIEAAIIKNKVYNEFDELSILADKYYGKSMLADY
                     YMALMYEKKEDYKRAQKKYLSAFQKEPIGDLNKDMMYDKSEEMKQMQQ"
     gene            complement(9880..10842)
                     /locus_tag="P5335_01575"
     CDS             complement(9880..10842)
                     /locus_tag="P5335_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRZ73598.1"
                     /translation="MKQKIGKWLSILLPILLGLALVIYTYSKFTPEQIEEIKLQFKNA
                     DYFYIYISLLISALALWSRGYRWKYTLEHLGYKPKISTNVMAVCIGYFINMSIPRSGE
                     VSRALILKKYEDVPFDKAFGTIIAERVVDSFFLLSFIGLAFFLELDVLKAFVLDKIPF
                     QKLMLILGTGTFIMIGIILMFRYSNFRLIQTIKQKISGLMDGVFSIWKMEKKWAFLFH
                     SLLIWASYFFMFYITIFALPETSNLSLSTVVISFIVGGLAITLTNSGFGAYPFLIAEI
                     LLLYNVPETSGTAFGWLVWTSQTLMVVILGTLSFLLLPILNKSK"
     gene            complement(10847..11197)
                     /locus_tag="P5335_01580"
     CDS             complement(10847..11197)
                     /locus_tag="P5335_01580"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRZ73599.1"
                     /translation="MQIQVVKSKIHRVKVTGADLNYIGSITIDEALMDASNLVEGEKV
                     SIVNINNGERLETYVIKGNRNSGEITLNGPAARKVQKGDIIIIIAYGILDFEEAKTFK
                     PSLVFPNEKDNSLT"
     gene            complement(11211..12059)
                     /gene="panC"
                     /locus_tag="P5335_01585"
     CDS             complement(11211..12059)
                     /gene="panC"
                     /locus_tag="P5335_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRZ73600.1"
                     /translation="MLIFNKQSLLIEHLTPFWATKTTIGFVPTMGALHEGHLSLLSQS
                     LKENEISVVSIFVNPTQFNNPEDLAKYPRTLERDVEKIASLNAKVLIYAPSVEDIYHG
                     NTVSQSFQYDGLENQMEGKHRPGHFDGVGTIVKRLFEIIKPTHAYFGEKDFQQLQIVK
                     KLVHKNNIPVKVIGCPIHREANGLAMSSRNERLSTIERKESALIFKTLSKAKKLFGMK
                     SAKEISKFVTKTFENHPIIKLEYFEIADEITLKSCLRKSKNKKYRAFIAVFVNNIRLI
                     DTISLN"
     gene            12168..12974
                     /locus_tag="P5335_01590"
     CDS             12168..12974
                     /locus_tag="P5335_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRZ73601.1"
                     /translation="MEDKRILYVSSEVVPYLAENEVSIMSYDVPKMVNDQGGQIRIFM
                     PRYGNINERRHQLHEVIRLSGMNLVVNDLDMPLIIKVASIPKERIQVYFIDNDEYFKR
                     KATFTDEEGKLYDDNDERAIFFAKGVVETVKKLNWVPDIIHVHGWLASLLPVYMKHYY
                     KDEALFSNTKIVTSVYAQSFDGALNSELLNKIKFDGVPEDSISVLKEPNYDNLIKVAI
                     QHSDAVIIASEHLSSSLTKFIESSEKPFLSFFPKEDYAEHYLNFYKNKVL"
     gene            12989..14626
                     /locus_tag="P5335_01595"
     CDS             12989..14626
                     /locus_tag="P5335_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HRZ73602.1"
                     /translation="MRTKKIIQFSSLVVLSFFLFSCDKDYNTLGADIIGDNHYTFGTP
                     DTEATVVAYNEDLGVVQSNNLVVNPLGIYKNTAFGKQTAHFATQVLLSEPVPTFTIID
                     EDPEILDVTLYVPYFSTRGTTTDDVTTYTLDSVYGTSKIKLSIYENGYLMRDFDPASN
                     FTAAQKYFSDQTNDFEVNKRGAAADGTSAANGERLNNGEDVEDNDEFFFDAAQIADTT
                     TNAEGEETITRSAPGMYLKLNKAFFQKKIIDGYNQGKLNNNNIFRDYFRGLYFKMEDS
                     PAEPTGSNSLAMINFKAGKITIKYKEDKAVEGEETPERVEKSMVLSLSGNTVSLLNQE
                     RTPNYQSALTNASSTLGDEKLYVNGGAGSMAIIDLFGRDASGESAELEQYRAKDWLIN
                     EASLTFRIDREEMGNNQEPYRVYLYDVTNDRLILDYFTDQTTNTSKPKLGKRVYSGMI
                     VKEDSKGTYYKIRLTNYISNLFKNPDSTNVRLGLVVTESIAITDNYAIKNQTASGLKK
                     VPLASVLNPLGTVLYGSKSSVPLDKRLKLEIYYTKPD"
     gene            14638..16488
                     /gene="glmS"
                     /locus_tag="P5335_01600"
     CDS             14638..16488
                     /gene="glmS"
                     /locus_tag="P5335_01600"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRZ73603.1"
                     /translation="MCGIVGYIGNRDAYSIVIKGLKRLEYRGYDSAGVMLYDGKDLKI
                     SKTKGKVTDLEAVSTKDNNFFGSIGMGHTRWATHGVPNDVNSHPHLSNSGNIAIIHNG
                     IIENYEPLKKELIKRGYVFHSDTDTEVLVNLIEDVQKKEGLKLGKAVQVALNQVVGAY
                     AIAVFDKNKPDEIVVARLGSPLAIGVGEGEFFVASDASPFIEYTSNAIYLEDEEMAIV
                     RLHKPIKVRKIKDDTLVDPYVQELKMNLEQIEKGGYDHFMLKEIYEQPNVIKDTYRGR
                     LLSNKGIIQMAGVEDNLEKFLNAERIIIVACGTSWHAGLVAEYVIEEFARIPVEVEYA
                     SEFRYRNPIINKKDIVIAISQSGETADTLAAIKLAKENGAFVFGVCNVVGSSISRETN
                     AGAYTHAGPEIGVASTKAFTTQITVLTLIALRLAKAKGTMNNSEYHRYLMELEMIPEK
                     VTEALVGTNEMAKKIAEIYKDSPNCLYLGRGYNFPVALEGALKLKEISYIHAEGYPAA
                     EMKHGPIALIDESMPVIVIAPKMGHYDKVVSNIQEIKSRSGKIIAVVTKGDTQVRELA
                     DHVIEIPETSDALSPLLTTIPLQLLSYHIAVLRGCNVDQPRNLAKSVTVE"
     gene            16765..19644
                     /locus_tag="P5335_01605"
     CDS             16765..19644
                     /locus_tag="P5335_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ73604.1"
                     /translation="MKVYLFILTLFFCSITFAQTSISGNVVDEKNQPIPGASIKIVGE
                     AIGAVTDFDGNFTLTTQKKPPFSVEVSSIGFTSKTVAISANNQKISVVLSDEETRLDE
                     IVVSASRAPERIRESPVTIERMSVKEIKKSASVTFYDGLENLKEVHMNTSSMSFKSIN
                     TRGFATVANTRFMQLVDGMDNSSPLLNFVLGNLIGVSEIDVQSVELLPGASSALYGAN
                     AFNGILFMTSKSPFTDEGVSAYAKVGQTSQEAAGNNLYYDYGVRVAKAFSPHFAAKAN
                     FTFMNGTDWYATDYRDRDLVNNVGIDRTDPNYNGINVYGDVVSTNIKSVASRLLNAGA
                     ITPAQFTVFNAILPNQNVSRTGYNEVDITDNKVQNAKIDFSFHVRPWATSSSEWAKDI
                     EFIWQSKFGFGNTVYQGANRYYLNGFQMSQHKFEVTSKNFFLRGYVTSEDGGNSYDME
                     FTAVNINRVWKPDNTWFGQYAATYVQSTLGGATPDQAHAAARNVADTGRFLPGSAEYK
                     NAFNQVTADPDVLTGSKLVDNSKIYHSDANYNFRDIIKIAEIQIGGSYRKYDLNSFGR
                     IYTDTPENGPIDYDEYGAYVQLQKKFLQDKFKFTGSVRYDKSQNFDGNYSPRVSLVFS
                     PDEQKRHNFRGSFQTGFRNPSTQDQYIGFNVGNAVLIGSAADNLDRYSEVRPVSTATG
                     QAFAGGESVVMTGQNAYYNSYTETSVRQFAALAGSDPIAASALLKRTDVDLVKPETVK
                     AFELGYRGQFEKFSYDLNGYYNIYNDFIGNLNVVAPLYGTAQDAPDPTAGPTNAGTQS
                     LHALQTGNIRAYQLYTNTDIEIISLGFGAGVSYKIYKDFEIGANYNYAEFDFDQEKDP
                     SFEAGFNTPKHRVKGSLSNDKLFPNFGFNVSARWNSEYLWQSTMVDGMISEATVIDAQ
                     INYGIPKLKSVLKVGATNIGGKEYQQVLGAGAIGQQFFVGITINQ"
     gene            19669..21156
                     /locus_tag="P5335_01610"
     CDS             19669..21156
                     /locus_tag="P5335_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G-D-S-L family lipolytic protein"
                     /protein_id="HRZ73605.1"
                     /translation="MIKNIKWLFLVSLTIVACSDDETVVVNNTSDGLPLTAGSADFSN
                     YVSLGNSLTAGYCDNALFIEGQANSYPAILADQFALVGGGEFKIPYTNDNTGGLLLGG
                     NVIAGPRLYFNGAGPAPVTGIPTTEVSTVLAGPFNNMGVPGAKSFHLLAPGYGNVAGV
                     ATGQANPYFVRFASSPSTTILADAAAQDPTFFSLWIGNNDVLGYATSGGSGTNQTGNF
                     NPATYGPNDITDPNVFANVYTALVDGMTANGAQGVVANLPYVTTIPFFTTVPHNPVPL
                     NAATAAQLNAGFSAYNNGLLFAQSVGLLTADEVTRRTISFTASTTNRVVMVDSYLTNL
                     AAYGIPSYRQATSEDLVVLPARSFIGTAVGGNPAQINGVSVPLADNWILSKDEILEVK
                     TATDAYNATIQSIASAKGLAFVDANALMTQVANGGITADNFTVTSTYVTGGGFSLDGV
                     HPSPRGYALISNEFIKAINATYGSNFKGVSLGSYRILYPENPDNF"
     gene            21380..22891
                     /gene="atpD"
                     /locus_tag="P5335_01615"
     CDS             21380..22891
                     /gene="atpD"
                     /locus_tag="P5335_01615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRZ73606.1"
                     /translation="MSKVIGKVAQIIGPVVDVVFDSKIAELPKIYDSLEIKRKDGAIL
                     VLEVQSHIGEDTVRTISMDSTDGLSRGTEVEATGAPIQMPIGPDVYGRLFNVIGDAID
                     GLGNLPKGGANGMSIHRPAPRFDQLSTSTEVLFTGIKVIDLIEPYAKGGKIGLFGGAG
                     VGKTVLIQELINNIAKGHGGLSVFAGVGERTREGNDLLREMLESGIIKYGEEFMHSME
                     NGGWDLSKVDKPGMRESKATFVFGQMNEPPGARARVALSGLSIAEYFRDGAGSDQGKD
                     VLFFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGAMQERITSTNKGSITSVQ
                     AVYVPADDLTDPAPATTFAHLDATTVLSRKIAELGIYPAVDPLDSTSRILTAQILGDE
                     HYNCAQRVKEILQKYKQLQDIIAILGMEELSEDDKLAVSRARRVQRFLSQPFHVAEQF
                     TGIPGVLVDIKETIKGFNMIMDGELDHLPEAAFNLKGTIEEAIEAGEKMLAEA"
     gene            22953..23234
                     /locus_tag="P5335_01620"
     CDS             22953..23234
                     /locus_tag="P5335_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRZ73607.1"
                     /translation="MILEIVSPEATLFKGEITSITLPGVDGSFQILNNHAPIVSILKQ
                     GTVKITAPSFKINKEVADKFTKVNDQNYTIEVTSGTIEMKNNKVIVLAD"
     gene            complement(23263..24786)
                     /locus_tag="P5335_01625"
     CDS             complement(23263..24786)
                     /locus_tag="P5335_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRZ73608.1"
                     /translation="MKDSPVNSLLTALIQFDKSSNTAVYIQIAQQIINAIQRGYLSAG
                     TALPGTRTFKELFQIHRNTAVAVYDELASQGWVEIIANKGTFILDPKPKKTALKAQSN
                     KIGDVHHYPKHTGFPFETSFLLSSTNEYSEAKYSLNDGQPDLRLHPVYEFSKWYSASM
                     KRKSLISKWNPNSKSKNSAFENQLCNYLNASRGFHVQPQNVISTRSTELSLYIISQLV
                     IRKKEIVLVGQLSNHNANMIFQEAGATIITIPIDENGLDIDYIEKHFTKGKIRCVYIC
                     SNRDYPTTYSLSAERRIKLMELAKAYQFAIIEDDYDYDFQLEGTAMLPMASADANGMI
                     IYLGKLGQSFLPSFQTGFVVAPENLIAEAKNYLQMVDRQGDLIQEQMLSELIQEGEIH
                     RLLKKNIITYKRRRDYLCECLNKYFNDTVYFKKPTGGLAIWITFQPSISLLQLAEEAS
                     KLDLFLPKTILYQDKKTCAIRLGFGHLNEEEIEIVISKLQLAYTVITTPKIKKAPRW"
     gene            24864..26684
                     /locus_tag="P5335_01630"
     CDS             24864..26684
                     /locus_tag="P5335_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ73609.1"
                     /translation="MKRRNFLVLFLVFTFIVTAQENSQRIDTLSEVIVSSTRIDIPFS
                     QNSRTIQLITSEQIKQSGVTNVADLLLLISGVDVRRRGTSGMQADLYLRGGSFDQTLL
                     LIDGIKVEDAQTGHHTMNLALPIEVIERIEIIKGPAARVFGQNAFTGAVNIVTKKALS
                     NKFTLQGQAGSYEQLNLSATIMQDYEKTSHVVQFSRNISEGYRYNTDYDNQNYFIKST
                     IKSSEMPIDILATFSERKFGANGFYATPSAINQYEETQASLVGVQSVFTKGNVTFKPR
                     MYWRRNQDEYVFVRDNPEIYRNLHITNKVGMELNTAIQSKWGTTGVGVDLAKVYLSSN
                     NLGDRDRFMGTFFLEHRFLLLDSKLDITPGFAANYYTDFDGNFFPGIDIGYQVSKHFR
                     TYANVGKTYRIPTYTDLYYSDPNTLGNEDLKQEEALSEEIGVVFTQNKFDFTVAFFNR
                     DSKNLIDYTKELESDKYQANNIRSLNTKGFEVGSNYKYTWFSFAQRINLGYTFIEDDI
                     QEIDAAFSRYSINSANHQFTGQWNSQFFQNISQTISYRYVERTSGESYGIVDANVVVR
                     MKAFEFSLVANNIFNVAYSETNLVPMPKGNLLFGVKYDFE"
     gene            complement(27151..29589)
                     /locus_tag="P5335_01635"
     CDS             complement(27151..29589)
                     /locus_tag="P5335_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73610.1"
                     /translation="MRVKLLISALLMTFIISVQAQNSGDYRSRISGAWTTAATWQTFN
                     GLIWVTATTYPGQNTSTASVLIRNGHTLTMGTNNVYLNQTGIITVSGQLNLNGINVSP
                     GSTFSFNTPSIIVTAIPAGRIKFDNKVKLMLPENASIQVSVGGLVGDCNHLQQIYIGT
                     FVIAYCRGGGSSALTFQELMEIGGSIHSVITTSPITCSSTSATITGSYLGSRYDFEVT
                     TSWKVVDPLNNTFYPTGDSFELPLDLNGVYNITYTCTINYFNNYVTNSNELKIPNAVS
                     TWTGSWNPVPPTELSKVRIAANYDTALNGSFSACSLNIEAGKTMQIAPNTYLEVENNI
                     VNNGAMVVESSGSLIQKENTATFTGNPITIKRISRPMRGLDYVYWGSPIAESYAIPTA
                     FDKKYRWNLTGAQDGAWVSITTPPPVGNGFIARVSQAYVPVNASPKTIEFPFVGLPNS
                     GPISVYANVFDGGDVNTDSGNSILLANPYPSAIEANALLLQHPQIVKLLFWTSVTVYT
                     GTGQYQVADYATWNLSGGTKPLSSTDESLTPKGYIASGQGFFAQVTADANITFNNSMR
                     IKDNNTQFYRTAQPVKENSETEKHRLWINLSNNKDAYRQMMVGYIEGATNALDTQYDG
                     SSFTQNEIDIYTILGKEQLAIQGRALPFNPQDIIPLGFKVTNEGKYTLALGHSDGLFS
                     DYKTMVLKDKLTNSYHNLNEGSYTFDTKAGTFNNRFELYFTEQNSTETVTQLPNNDCV
                     IWSAEKQIHINSTGFPIESVEVYDVFGTLIWSQTGVKDIAVVTNQLNTANPFLIVKVT
                     DANRQVITKKIIFK"
     gene            complement(29776..30267)
                     /locus_tag="P5335_01640"
     CDS             complement(29776..30267)
                     /locus_tag="P5335_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRZ73611.1"
                     /translation="MYKTVASVSSTSLPQIVQKMEYYCSYQERCHLEVMQKLYGFALT
                     QQERDEVVVHLIQHNFLNEERFAKTFAISKFHQKKWGKIRITNELKQRQISTYLITKG
                     LQEIPSHEYEETFESLTEKTWQTITEKNALKKRKKFCDTLLRKGWESELVYEAVKKLE
                     KNS"
BASE COUNT         9411 a         5072 c         5764 g        10020 t
ORIGIN      
        1 aatagttttc attattaaca ggttacaaaa aaaccgcccc aaatttcttc aaggcggttc
       61 aactacaatt caaccaaaat taatttacca ctacatagcc ttttgtttta gcaatactgt
      121 gaatagaagt ggtgatggct tgacctaaat cgtcttgtgt tttggtcttt ttcgattctt
      181 catcaataaa tgcttggcgt tttttagaaa gttcttccat ttctttttgt atggtttcgc
      241 gttcttttgt tttttgagtt acaataactt taatttcttc tttagatttg ttttttaatt
      301 ccgccggaag ttcttctttt ttaaggttgt ccaatgcttt ggcattcgtt ttggctttgt
      361 ctaccaaatc ccagctttca tttttatata cggtttttga tttacttact gctcgttcgg
      421 tataattggc tccggaaatt gactgagcat tggcatcttg cacttcttga gccatttttt
      481 tctccacacc tttcgaacca tacccaatgt aagtagtatt caatcgcaca ttgcattgtg
      541 aaatttgatc atcataagga gtcacgatgt accgaacttt tgcattggaa tcgatgttga
      601 aatatttgcc ttgtcccaca tcggccccgt ctttccataa tccacgaatg ccttcctcat
      661 aactaccgca atgaatagtg ttaacataaa ttcctttttt caacgcatca gcaatagctt
      721 ctttgtaatt aatactacct tggttaaagg gttcatttcc ggcaatgtaa aggagtttca
      781 tgttttgcaa tccgtcattc caagttagtt tttgagaagc atcttgaatt accgctccac
      841 aatattcact tccaccattt gttcgtaatg aaaacaattt ttcagaaatt aagtctaaat
      901 ctgtcgttaa aggggcaact tgtctgatat aattcgtgtt tttgttcaac ccgtcatttc
      961 catattcata taaagcaatt tcaatttccg gcgttttacc ttcaaatttt agggtagtca
     1021 acgtattgac aatgttccat aatcgagatt ttgcctgttc gattagtccg tccatactgt
     1081 tggaagtatc caataaaata gcaacttgaa tttttccatt ggtttcatct gtggcaatag
     1141 caggttgatg ggtttctgtt aaacgaatag gtgtttcagc caggttgttt ttcttttcac
     1201 agctaaacaa tagtgttgag gtaaacaaag cgaaggttat aattatattt ttcatgatac
     1261 tgttttttaa aattaatgtt gaagtttttg aatgtcattt tccaccgaaa gaatcatcaa
     1321 acaagtggca gtacaaaagg aaggactggt aatacaatga tgaccgctcc agctaccgtc
     1381 tgcgttttga atttgcatca ttcggccacc aatattgtcg taccattttt tccactcatc
     1441 atcatggttt acaatcatgg attcgccggt ttgtaaaaaa cttaggaatt cttctccgcc
     1501 gttgttgcca aatccggtta aaacatcttc attcattgct tttactttgg ctgatttata
     1561 tactttgtct gccacatcat acgatttagc tttttcatca gacacgccaa ttcgtttcaa
     1621 atttgcttcg ttaagttggg catctttttc gatacgacct tcctcttttg cctttttgaa
     1681 aattttatcc gcgtctttag cttcttccgc acttccacga accgaactgc tcacggcata
     1741 cagcatgatt ccggcaccat cctctgtttt tgccgaagca ttagccggat tgtaattgct
     1801 tttttggtag tctcttgctt tttgcctttt ttcttggtct actttgattc cttcattttt
     1861 gcttgccatt tccaaacttg aattggcaaa agaagattgc aaaacacctg cccagccgga
     1921 gcctcccact ttaccggaat tatccattga tttttcgatt ttatccacac aaatttgtat
     1981 tgcagctttg accttcgttt tcatcgcttc atttttaagc gtcggaagca gttggttaaa
     2041 aaattgcagg gttagaattg catcgatatg ttgtcccaat tttccttgaa tttgagttcc
     2101 tcggatagct gtgatatagg gtaaattttg gtttttatca atttcattta aaagaaagtg
     2161 tactgctttt tccagggttt ctttgtgttt ccctgattcc atagagtaac cacagcgata
     2221 caaggccatt gccgacatag ctgtagtggc aggatcggaa gaaacggcat gaggatttct
     2281 ttccccttga ttactttgta aaccggcacc ccaaccgcca tctgtatttt gagcttctaa
     2341 aagccataat tcacctttag aaatggtttc ttgaaatttc tcagcggttt tagctaccgc
     2401 aaaaccttct gaggtttgtt cgcccattaa ggtcataaat acacagggtt tttcttcttc
     2461 ttcagtgata tcagtttcta aaatggctaa ctcttttgat ggagaagcat ttattggttc
     2521 ggtttttgga tttgtattcc aaacataaat taacgggatg caacttgtta gtgctgacaa
     2581 tccccaaatg atttttgctt tcataattta tcgcttttat tgattttttg ataaaattag
     2641 aaggttgaac cattcgtttt tttgagactt ggtgaaactg gtttttgact tggtgaaaac
     2701 gttttttaaa cccaaattaa ttttcgtact ttacactata atataaaagg catgtataat
     2761 caactgaaaa tactattggt agttttattt ttccctttga tgatggtcgc tcaggatagc
     2821 actaaaacca aagtgagtaa aacgtctgtg agtaaaaagg caaaagaagt agaacaaacg
     2881 ttatctaaaa gtaatccgga aagtatcgca gataaatatg tggaattggc gaatgagttg
     2941 gataaaaagg gtgattacgc caaagcggaa gattatttga aacgagcttt gcctatttat
     3001 caaaagcaaa aaatcaaaga caaaatagcg agtacaacgc gggcgattgc tcaaattcag
     3061 gaaaagcaga ataaagtaaa agacgccatc aaaagttatc aatcggcaag tgaagaagcg
     3121 gttgatgttg ggtattcgaa agtgaattcg aatgatgcaa gccgacttca aaacactaat
     3181 ccaaaagcac aagcagaata tttagattcg aacatcaaaa ttttagagaa agaaggtaaa
     3241 atggatgatg ccgccgaagt ttataaacaa aaagcggaag tcgattttaa gtcggaaaac
     3301 aaaggcatgg cgattgaaaa ttatgaaaaa gccattgctg tttctaaaga taaaccggaa
     3361 gaagtgttga aattgaaaag cgaaatttcc aaagtatatg cctcagaaaa caatttagac
     3421 aaagcgattg aaattacaaa aaacaccatt tcggaagcgg agaaattagg cagtatttcg
     3481 caacaaattg aacagcaaca aacgttggcc gacttatatt ttaagaatga aaatcaagat
     3541 gatgcgattg ttttactgga agaaacctat gccttggctt taaaaaacgg tcgcactttt
     3601 gacgctaaaa aagcgatgtt ggctctttcg gattattaca gacaacaagg caatcaaaca
     3661 aaagcgattg aattatatga ccaatttctg aatgattttg atcggatggt gcaagccgac
     3721 agtactttgg tggattcgaa aatctttcag gtaacggagg aaaaaatcag gcaattggaa
     3781 aaagaacgtg cgttgaaaga tgagttaatt acgaagaaaa acaaatttaa ttatgtcttg
     3841 attggttcgg tgattttgat gttgatttta ttgggattga tagcgaaagc gttgtttttc
     3901 atcaaaacca agaacaagaa gattgcgtta caatcgcttc gacgggaaat gaatccccat
     3961 tttattttta atagtttgaa tagtgtgaat caatttattg ctcaaaataa tgagttggaa
     4021 gccaataagt atttgacatc ctattccaat ttgatgcgaa atatgatgga aacgtccaac
     4081 aaagatttta tttcgttatc caatgaacta gaacaaatta aaaagtattt ggatttggaa
     4141 catttacgtt ttcaggacaa atttgaatat gaattggagg tggatgaaca attggatgtc
     4201 gatgtgattt tggttccgaa tatgttgatt caaccgcatt tggaaaacgc catttggcac
     4261 ggattacgtt accgagaaac caaggggaag ttgatagtca cttttgcaga agagaatcaa
     4321 ttgataaaag tatcaattga agatgacggc attggtgtag aaaaaagtcg cgaactgaaa
     4381 acacaaaacc aaaaggtaca cgaatccaga ggcttgaaca atgtggaaga acggattaat
     4441 ttgttaaacg atttgtatca tcaacagatt tcctacacga tttcttcggg aacagatgga
     4501 aagggaacga tagtcacgct ttatttttct aaaaccacta aagtagcatg aacgattttc
     4561 aaaaaataaa aagtgtcatt gttgaggatg aatcggctgc tcgcgaagcg ttgaaaaatt
     4621 acttggcaaa atattgtccg caaatcgaaa tcattggtga ggcacaaaac agtcgggaag
     4681 cgattccgtt gttgcacgac ttgcaaccgc aattggtttt tttggatgta gaaatgccgt
     4741 ttggcaatgc atttgatgtg ttggaaggtt gtaaagattt gtcttttgaa accatttttg
     4801 tcaccgcttt ttcagaatat tcgttgaagg cgttgaatca aagtgcggca tattatttat
     4861 tgaaaccgat tagtattgaa gaattgattt tggcggtgaa caaagtgcaa caacaattga
     4921 tgaagcagga attgtttaac cgaaatcaaa tcattgttca aaatttccgt gagcctagaa
     4981 tcgagaagca acaagttatt ttgcccacct tagaaggttt tgaagtcgta aaaatggaag
     5041 aaatcgtccg tttacgtggc aatggcaact ttacggatat acatttggcc aatggttcta
     5101 aaaaaatggt ttgtcgtttt ttaaaacatt ttgatgaact gttggacttt ccttttatgc
     5161 gggtgcacaa atcgcacatc atcaatttga attatgtgaa atcgtatcac aaatcgttgg
     5221 gtggttatgt aatgcttttt gatgcctctg aggttgaaat ttcacccaat tataaagagg
     5281 agtttttgag aaaattcaac tagtttattc tattaaaagt cagcttcctc tatttcaata
     5341 gaatctatgg ccgtggagtc tttttctgtt tttaatcgta aataagtttt tgaaattcct
     5401 ttaattttaa ccggaatcgc tgctgtaata ctatcttgag cttcaattaa accacggctc
     5461 aacataatgt ttttatacat tttttgttta cttacggcat aaggtttttg gttgccttcc
     5521 ttgtcaaaca gccacatgaa ttttttagga ctaggcacaa tggatgctaa aaataatgat
     5581 tcattgaggg ttaattctct cggagttttc tcaaaataaa attgagccga ttcaccaatg
     5641 ccgtacacat ttggacccca ttctattaca ttgagataga cttctaacat tctggctttt
     5701 ggcactaatc tattgttttc taaaagataa actaaaagaa tttcttccag ttttcgggaa
     5761 agtgtttttt cacgtgtcag aaatacattt ttaaccaatt gcatgctaat tgtactggct
     5821 cctcgggtaa actttttagt tttaatattt ttagcaatgg attgtttgaa agcatccgtg
     5881 ctaaatcccg aatgattgta aaattgagga tcttcaaaag ttaaaactgc tttttgtaag
     5941 taaggagaaa tttcctcaag cggagtaaaa taaggatttc cggctcctac caaaacagaa
     6001 cgttgtttac gtccgttttc aatcgcatta tatacaaatt cgccattcaa ttttgacaaa
     6061 tcagcttctc cgtatttgag aatttttaag ttttctttat tcagtttact ttcaaaaatc
     6121 aaagcctccg gattgttttt attaaattca aagtccaatt gataatcaaa agcaccttga
     6181 acttgcattc cttcaaaatg agcaaacaaa cctgttggaa gtgaattgac aaaatcttgg
     6241 gctttcattt ttggcactcc tatttttagc gtataaatgg tatctttttc agtactgtac
     6301 tttacaaatg gacgaagtgt aattttattt agtttcgcag aagaagaacg atcaatagca
     6361 ataaaattac tgcctaagat aaaatggtaa tcaatttgtg ttttttcaaa aataacatct
     6421 ttggaggcaa ttttaggatg atttaccgtt aaatcttcta tttctgtgac tccatcaata
     6481 tacaacttac cctcttccat cgttaaatta tttaaaacga tgtgaatagc agaaaacgaa
     6541 gaaaataatc caaatcgttc ctcgatgtaa ggcattctga ttttagttga atcataggtt
     6601 gaaaatttca aatcagcttt tttaagtcgg ggatcagcat agcctttcat ttgccagttt
     6661 tgactaaacg aattggtttt gaccttaata gaggattgaa gttggtggtc ttccaaggtc
     6721 aatttgttta ggtgtaaatt gacttttcta cccatatcat ctattttcag ggataagttt
     6781 gaaagagtca tttctgccgg aatcaaatta aacactttag tcaatagtcg atttgctaaa
     6841 gctgcataat ctcttttctc tgtagttttt tcttcttcag gatttttttt caaaaaagca
     6901 tcaaaattcc gtccgttttt attttttatt aattgaacaa aaccattact catttccatg
     6961 ttattcagcc ggatattgcc ggttaataat tggaaaaaac tataattagt ttttattttt
     7021 tctacggata ataaggtgtc ggcatttttt ggaattaaaa gaatatccac taattctact
     7081 tctgtcagtc cactaaaagc cgcttttttt actgaaaatc ggcagttaaa atcagtttca
     7141 aatttagttt gaattcgtgt gattgatttt tgtaataaga catcacgaaa aaagtaaagc
     7201 gtaataaaaa tcaaaaccaa aaaaaccaaa aatagcttta agcttaagat aattttttgt
     7261 tttttcgttc tcatttatgg attagtatga tattgtagtt tataaaatta cccaaataat
     7321 tgttcgacaa catcctcaat ttttgcgact aattttactt caattaaggt gtttttcaag
     7381 gaaattttat tgtattttga tacaaaaata gttgaaaaac ctaatttttc tgcttctaaa
     7441 atgcgttgtt ccactcgatt taccggacga atttctccgg ccaaaccaac ctctccggca
     7501 aagcaaaagt ttttccccac cggaatatct tcatttgacg ataaaatggc agctactacg
     7561 gccaaatcta tagcgggatc atccacagaa attccgccgg taacgtttaa aaaaacatct
     7621 tttgccccca accggaatcc ggcacgtttt tctaaaacag ctaaaatcat gtttaggcgt
     7681 ttggcattgt aaccggttgt gctgcgttgt ggcgttccat aaacggcagt gcttactaac
     7741 gcttggattt ctatcatcaa aggtcgcatt ccttctaaag tagaagcaat ggctgttcct
     7801 gaaagttctt cgtctttgtg tgatattaat atttccgatg gattggcaac ttcccttaaa
     7861 ccagaacctt gcatttcata tatcccaatt tctgccgtag aaccaaaacg gtttttgagt
     7921 gaacgaagaa ttcgatagac atgatttctg tcgccttcaa attgcaaaac agtatcgacc
     7981 atgtgttcta aaatttttgg tcctgcaatg gttccgtctt ttgtaatgtg tccgataagc
     8041 aaaaccggga catttgtttc tttagcaaac ttgattaatt cagcagtaca ttcccgaatt
     8101 tgagaaatac ttcctgctgt agagtcgata taatccgtgt gaagggtttg aatggaatcg
     8161 ataattacaa tttcaggttc gatttcctga atttgtttga atatattttg cgtttttgtt
     8221 tcggttagaa tgaagcaatt gtccccgtta ggcgtgattc gttcagcacg cattttgatt
     8281 tgtttttgac tttcttctcc ggaaacataa agtgttttaa agggtaattt taaagaaatc
     8341 tgcaataaaa gcgtactttt tccaataccg ggttcacccc ctaaaagggt taatgagccg
     8401 ggtactaatc ccccacctaa tactcgattt agttcgccat cggtcgtgtc taatctgatt
     8461 tcctgactgg aatcaatgtc ttttattcgt aaaggttttg gagctttttt aacttcggaa
     8521 gaagagggtt tccaacttgt tttttcttct ttttgaatga cttcttcaac aattgtattc
     8581 cattgttggc aggcatggca ttggccttgc catttggcat attgtgaacc gcaattttga
     8641 caaaaaaatg aagttttgac cttcgacatg attattgttg catttgtttc atttcttctg
     8701 atttgtcgta catcatgtct ttatttaaat caccaatcgg ttctttttga aaggcactta
     8761 aatatttttt ttgagccctt ttatagtctt cttttttctc atacatcaaa gccatataat
     8821 aatcggccaa catacttttt ccataatatt tatcagctaa tatggaaagc tcatcaaatt
     8881 cgttgtagac tttgtttttt ataattgccg cttcgatggc cttgaaatca tttaatcgaa
     8941 tgggcatttt gatatttaaa gatttttcca aaacatcata ttttgttttt aaatattcca
     9001 cataaccata ttctaatttt acaatttttt cattaaactc aaacgtagtg atgggttggt
     9061 aaacggcaaa aaactggtac aaagcatttg gaatcgagtg gagcactaag gaataatggg
     9121 aagctcctat gaattcatca aatttataat ttaacgttgt gtttttgatg actttagctt
     9181 tttcatctaa ttctttaatt cgtttctgca ttttttttac atcaccatca gccgttgaat
     9241 gataatagta aatcggttta gttataaggc ctagacgttc cggaattcga ttttccatat
     9301 ccgttgctaa gtcaggactt aatgtaatgt aggcgtcaaa aagcggatta tctttgtata
     9361 aataaaaatt tgtaaatccg gccgtagtgt catgaccggc aataatttta aacggagcaa
     9421 ttcggtacgt tttttcaaga taaggcatta gttcagtacc tataaaatca aaaaaattgg
     9481 ctccggtaga atcaggtaat ccggtttctt catcaaattc actatcggca taacgttctt
     9541 ccgttttatt ttgacaaatt cccactaaaa ttacttccgg taaatcatcc caataattcc
     9601 caaatttaag ggctccttga aatgggtcaa aaagatattc tgcatctaaa actaacaata
     9661 tagggtaatt acgattcgtg tccttttcat acgatggagg taaactgatg tagatttcac
     9721 ggtctgcacc taattttttt gacgtaaatg tttctattgt tttttgagaa aacaaaggga
     9781 tagaacacaa tacaataaca agtgtttgaa ttaatttcat gtggtatctt tttggaaatt
     9841 tcttgaaaaa gcttaatttt tcgaaagcaa gatagtaaat tatttgcttt tgtttaaaat
     9901 gggaagcaat aaaaatgaaa gtgttcctaa aatcaccacc attagtgttt gtgaagtcca
     9961 taccaaccaa ccaaaagcag ttccggaagt ttcgggaacg ttataaagta aaagaatttc
    10021 tgctattaaa aagggatagg ctccaaatcc cgaattggtt aaagtaatgg caagtccgcc
    10081 tacgataaat gaaataacca ctgtgcttaa actcaagtta ctggtttcgg gcaaagcaaa
    10141 aatagtaatg taaaacataa aaaaataact tgcccaaatc aataacgaat gaaatagaaa
    10201 agcccatttt ttttccattt tccagatgct gaaaacacca tccattaaac cggaaatttt
    10261 ttgtttgatc gtttggatta acctaaaatt agaatagcga aacatcaaaa taatgcctat
    10321 cattataaat gttccagttc ctaaaatcaa cattaatttt tgaaaaggaa ttttatccaa
    10381 tacaaaagct tttaatacat cgagttctaa gaaaaaagcc aatccgataa aagataataa
    10441 gaaaaaacta tctaccacac gttcagcaat gatggttccg aaggctttgt caaacgggac
    10501 atcttcatat tttttgagaa tcaaagcacg ggaaacttcg cccgaacgcg gaatagacat
    10561 attgatgaaa tagccaatgc aaaccgccat gacgtttgta gaaatttttg gtttataccc
    10621 caaatgttcc aatgtatatt tccatcgata tcctcttgac cataatgcca aagcactgat
    10681 taataaagaa atatatatgt aaaaataatc agcattttta aattgcagtt taatttcttc
    10741 tatttgttcg ggagtgaatt tgctgtaagt gtaaattacc aaagccaatc ccaacaaaat
    10801 tggaagaaga atggaaagcc atttgcctat tttctgtttc aagggattag gtcaacgaat
    10861 tatctttctc gtttggaaaa accaaagaag gcttgaaggt tttagcttct tcaaaatcta
    10921 aaattccata agcgataata ataataatat ctcctttttg aactttacga gcggcaggcc
    10981 cgttaagggt aatttcgcca ctattacggt ttcctttgat gacataggtt tctaatcgct
    11041 caccattatt gatgtttaca atagaaactt tttcgccttc cacaaggttt gaagcatcca
    11101 taagggcttc gtcgatagta atactaccga tataatttaa atctgctcca gtcaccttaa
    11161 cgcggtgaat tttggatttt acaacttgaa tttgcatggt gcaaaggtaa ttaatttaat
    11221 gaaatggtat caatcaatcg aatgttgttc acaaaaacag caataaatgc ccggtatttt
    11281 ttatttttcg acttacgtaa acaggacttt aaggtaattt catcagcaat ttcaaagtat
    11341 tctaatttga taataggatg attttcaaaa gtttttgtaa caaattttga aatttcttta
    11401 gcacttttca tgccaaataa tttttttgcc tttgataatg ttttgaaaat tagggctgat
    11461 tcttttcttt caatcgtgga taatcgctca tttctggaac tcatcgctaa tccgtttgct
    11521 tctctgtgaa ttggacaacc gattactttt acggggatat tatttttatg tactaatttt
    11581 ttgactattt gtaattgctg gaagtctttt tcgccaaaat aagcatgggt gggtttaatt
    11641 atttcaaaca accttttaac gatagtcccc acgccatcaa aatggcccgg tcgatgtttg
    11701 ccctccattt ggttttctaa gccatcatac tgaaatgatt gtgaaacggt atttccgtga
    11761 taaatatctt caactgatgg agcataaatt agaactttag cattcaaaga agctattttt
    11821 tccacatctc tttccagtgt tctggggtat tttgccaaat cttccggatt gttaaattgg
    11881 gttggattaa caaagatact cactacagaa atttcatttt ctttcagtga ttgtgaaagc
    11941 aaagacaagt ggccttcgtg taaagcaccc atcgtaggaa caaatccaat ggtcgttttt
    12001 gttgcccaaa atggggttaa atgctcgatg agtaggcttt gtttgttgaa aatgagcatg
    12061 tatttttaat taaatgagtt gcaaacttac tattttactt aaatatctgc gtaaattttt
    12121 gtaattttgc gtgattttaa aatctaaacg ataaagttca aaataatatg gaggataaga
    12181 ggatattgta tgtatcatct gaagtagtac cttatttggc tgaaaatgaa gtttccatta
    12241 tgtcttatga cgttcctaaa atggttaatg atcagggagg gcaaataaga atttttatgc
    12301 cacgatacgg taatattaat gaaagaagac atcaattaca tgaagtaata cggttgtccg
    12361 ggatgaattt agttgtgaat gatttggata tgcccttaat aataaaggtg gcttctatcc
    12421 ctaaagaaag aattcaagtc tattttatag ataatgatga atattttaaa cgtaaggcaa
    12481 cttttacgga tgaggaagga aaattatatg acgataatga tgaacgggca attttctttg
    12541 caaaaggagt tgtagaaaca gtaaaaaaat taaactgggt accggatatt attcatgttc
    12601 atggttggtt ggcttccttg ttgccggttt acatgaaaca ttattacaaa gacgaagcac
    12661 ttttttctaa tacaaaaatt gtaacttcgg tttatgctca gtcatttgat ggagctttaa
    12721 atagcgaact tttaaacaaa ataaaatttg acggtgttcc ggaagattcc atttctgtat
    12781 taaaagaacc caattatgac aatttaatta aagtagctat tcagcattca gatgctgtta
    12841 tcattgcttc agagcacctt tcttcaagtt taacaaaatt tatagaatct tcagaaaaac
    12901 cttttttatc tttcttcccg aaagaggatt atgctgagca ttaccttaat ttctataaaa
    12961 ataaagttct ataaatttac attttaaaat gcgtacaaaa aaaataattc aattttcttc
    13021 cctggttgtc ttgagtttct tcttgttttc ctgtgataaa gattataata ccttaggggc
    13081 tgatatcatt ggagacaatc attatacatt tggaacaccg gatacagagg ctaccgtggt
    13141 tgcctataat gaagatttag gtgttgttca atctaacaat ttagtggtta atccacttgg
    13201 gatttataaa aacactgctt ttggaaaaca aacggctcat tttgccacgc aagtattgct
    13261 ttcagagcca gttcctacct ttactattat tgacgaggac cctgaaattc tggacgtcac
    13321 gctttatgtt ccatatttta gcacaagagg gaccactacg gatgatgtca ctacttatac
    13381 cttagattct gtttacggaa cgtctaaaat taagttgagt atatatgaaa acggttattt
    13441 aatgcgggat tttgatccgg cttccaattt cactgctgct caaaaatatt tttctgatca
    13501 aacaaatgat tttgaagtaa ataaaagagg agctgcagcc gatgggacat ctgcagccaa
    13561 cggagagcga ttaaataatg gagaggatgt tgaagataat gacgagttct tttttgatgc
    13621 agctcaaatt gctgatacta ccaccaatgc tgaaggagag gaaactatta ccaggtcagc
    13681 tcccgggatg tatttaaaat tgaataaagc tttttttcag aaaaaaataa tagacggcta
    13741 taatcaagga aaattgaaca ataataatat ttttagagat tatttcaggg ggttatattt
    13801 caaaatggaa gactcaccgg ctgaacctac cggaagtaat agtttggcga tgattaattt
    13861 taaagccggg aaaattacta taaaatataa agaggataaa gcggttgaag gagaagaaac
    13921 accggaaaga gtagaaaaat caatggtatt atctttatca gggaatactg ttagtctttt
    13981 aaatcaagag cggacaccaa actatcaatc ggcattaaca aacgcttcct caactttagg
    14041 agatgaaaaa ttatatgtca atggaggtgc cggatcgatg gcgattattg atttatttgg
    14101 gcgtgatgcc agtggagaat cagcggaatt agaacaatat agagctaaag attggttgat
    14161 taatgaagct agtcttactt tccgaattga tagagaggag atgggcaata atcaggagcc
    14221 ctatagagtt tatttatacg atgtaactaa tgatagactg attttagatt actttacaga
    14281 ccaaactaca aatacttcaa aaccaaaatt aggtaaaaga gtttatagcg gaatgattgt
    14341 aaaagaagac agtaaaggaa cttattataa aatacgatta actaattata tttctaattt
    14401 atttaaaaac ccggattcta ccaatgttag gctaggactt gttgtaacgg aatccattgc
    14461 gattactgac aactatgcaa taaaaaacca aactgcctca ggtttgaaaa aagttccctt
    14521 agcttctgtt ttaaatcctt taggcacagt gttgtatggt agcaaatctt ctgtaccttt
    14581 ggataaaaga ttaaaattag aaatttatta tacaaaacct gattaaatac taaaaatatg
    14641 tgtggaattg ttggatatat tggcaataga gacgcttact ctattgtaat aaaaggatta
    14701 aaacgattag aatatagagg ttacgacagt gccggagtca tgctttatga tggcaaagac
    14761 ttgaaaatat ctaaaacaaa aggtaaagta actgatttag aggcagtttc aactaaagat
    14821 aataatttct ttggttctat aggaatgggt catacccgtt gggctactca tggagttcca
    14881 aatgatgtaa attcacatcc tcacctttct aattccggaa acattgccat aattcacaat
    14941 ggaatcatag aaaattatga acccttaaaa aaagaattaa tcaaaagagg ttatgttttt
    15001 cattccgata ctgatacgga agtattagtt aatttaattg aagacgttca gaaaaaagaa
    15061 ggtttgaaat taggaaaagc ggtgcaagtt gctttgaatc aagttgtcgg agcctatgca
    15121 attgcagttt ttgacaagaa caaaccggac gaaattgtcg tagcccgatt aggaagtccg
    15181 ctagccattg gtgttggcga aggagaattt tttgtagctt cagatgcttc gccttttatt
    15241 gaatatactt ccaatgcgat ttatttagaa gatgaagaaa tggccattgt gcgacttcac
    15301 aaacctatca aagttagaaa aataaaagat gataccttag ttgatcccta tgttcaggaa
    15361 ttaaaaatga acctggaaca aattgaaaaa ggaggttatg atcattttat gctcaaagaa
    15421 atttatgagc aacccaatgt cattaaagat acttaccgtg gaagattact ttctaataaa
    15481 ggaataatcc aaatggcggg tgtggaagat aatttggaaa aattcttaaa tgcagagcga
    15541 attataattg tagcctgtgg aacatcatgg catgccggat tagttgcaga atatgtaata
    15601 gaagaatttg caaggattcc cgtggaagtt gaatatgcct cagaattccg ttatagaaat
    15661 ccaataatta ataaaaaaga tattgtaatt gctatttctc aatccggaga aacagcggat
    15721 accttagccg caattaaatt agccaaagaa aatggtgctt ttgtatttgg tgtatgtaat
    15781 gttgttggtt cttctatttc aagagaaacc aatgctggtg cttacactca tgcaggtcct
    15841 gaaatcgggg tggcttctac aaaagctttt acaacacaaa taacagtgtt gaccttaatt
    15901 gctttgcgtt tggctaaagc aaaaggaaca atgaataatt ctgaatacca tcgctaccta
    15961 atggaattag aaatgattcc ggaaaaagta accgaggcac tagtaggaac aaatgaaatg
    16021 gccaaaaaaa ttgctgaaat atacaaggac tcacccaact gtttatattt aggtagagga
    16081 tataatttcc ctgtagcttt ggaaggagct ttaaaattaa aagagatatc ctatatccac
    16141 gcagagggat atcctgctgc agaaatgaag cacggtccaa ttgctttaat tgatgaatct
    16201 atgccggtta ttgtcattgc tcctaaaatg ggacattatg ataaagtggt tagtaatatc
    16261 caagaaatta aatcaagaag cggtaaaata attgctgtag taaccaaagg ggacacacaa
    16321 gttcgcgaat tagctgacca tgttattgaa atcccagaaa catcagatgc tttatctcca
    16381 cttttaacta ctataccatt acaattactt tcttatcaca tagccgtttt aagaggttgt
    16441 aacgttgacc agccaagaaa tttagcaaag tctgttaccg tagaatagtt aatttaaaac
    16501 agcaaataat tgaaagcaag agaaatcttg ctttttttgt ttttatacaa tttttattta
    16561 ttaacaacgc acaaaatagt atgtaagcat actattttga ggcttttaaa cgaaaacgtt
    16621 tgagattttt tcaaaaacct ctgtgatatt cagtaattac tatggttttc aaaaattgtt
    16681 aaattcgaaa atttagcacg ctttttttat taatatcaaa attatttgta ctttgctgac
    16741 gaaaactaac aaaactctaa ctttatgaaa gtgtatttat ttattctgac attgtttttt
    16801 tgcagcatca cttttgctca aacttcaatt tcagggaacg tcgttgatga aaaaaatcaa
    16861 cccatcccag gtgccagcat taaaattgtt ggcgaagcga taggtgcggt gaccgatttt
    16921 gacgggaact ttaccttaac tacgcaaaaa aaacctcctt tttcagtaga agtctcttct
    16981 attggcttca cttctaaaac agtagccatc agtgcaaaca atcaaaaaat cagtgttgtt
    17041 ttgtctgatg aagaaacacg tttagatgaa attgtagtat cggcttccag agcacctgaa
    17101 aggataagag aatcgcctgt tactattgaa agaatgagtg ttaaagaaat taaaaaatca
    17161 gcttcggtga ctttttatga tggattggaa aatttaaaag aggtacatat gaataccagt
    17221 agtatgtctt ttaaatctat caacaccaga ggttttgcca ctgttgcgaa tactcgtttt
    17281 atgcagttgg tagatggtat ggataattct tctcctttat tgaattttgt tttaggaaat
    17341 ttaattggtg tttcagaaat tgatgttcaa agtgttgaat tgttaccagg tgcctcttct
    17401 gctttatacg gtgcgaatgc ctttaacggt attttattta tgacaagcaa aagccctttt
    17461 accgatgaag gcgtttctgc ttatgcaaaa gttgggcaaa ccagtcaaga agctgccgga
    17521 aataatcttt actatgatta tggagtacgt gtagccaaag ctttttcgcc ccattttgct
    17581 gctaaagcta attttacgtt catgaatgga acagattggt atgccactga ttatagagac
    17641 agagacttag taaataatgt tggaattgat agaactgacc cgaactataa tggtataaac
    17701 gtttatggtg atgtggtttc aactaatatt aaaagtgttg cttccagatt attaaatgct
    17761 ggtgcaataa ctcctgctca atttactgtt tttaatgcta ttttgccaaa tcaaaatgta
    17821 tcaagaacag ggtataacga agttgatatt acggataata aagtacaaaa tgctaaaatt
    17881 gatttctctt tccacgttag accatgggct acatccagtt cagaatgggc aaaagatatc
    17941 gagtttattt ggcaaagtaa atttggtttt ggtaacacgg tttatcaagg agcaaacagg
    18001 tattatttga atggatttca aatgtcacaa cataaatttg aagtaaccag taaaaacttc
    18061 ttcttaagag gttatgttac ttctgaagat ggaggaaatt catatgatat ggaatttact
    18121 gcggtgaata ttaacagagt ttggaagccg gataatactt ggtttggtca atatgccgcc
    18181 acttatgtac aatcaacgtt aggtggagcg actccggatc aagcacatgc tgcagcaaga
    18241 aatgtggctg atacaggaag atttttacct ggttcagcag aatataaaaa tgcttttaat
    18301 caagtcacag ctgacccgga tgtattaacc ggttctaaat tggtggataa ctcaaaaata
    18361 taccattctg atgccaacta taattttaga gatattatta aaattgctga aattcaaata
    18421 ggtggttctt acagaaaata tgatttgaat tcatttggta gaatttatac ggatacacct
    18481 gaaaatggtc ctattgatta tgatgagtat ggtgcttacg ttcaacttca aaagaaattt
    18541 ttacaggata aattcaaatt tacaggttct gttcgttacg ataaaagtca aaattttgac
    18601 gggaattatt ccccaagagt atctttagtt ttctctccgg atgaacaaaa aagacacaat
    18661 ttcagaggat cttttcaaac cggttttaga aatccttcta cacaagatca atatatcgga
    18721 tttaatgttg gaaatgcagt acttattggt tctgccgctg ataacttaga cagatattct
    18781 gaggttagac cagtttctac tgctacagga caagcatttg caggaggtga atctgttgtg
    18841 atgacaggac aaaacgctta ttataattct tatacagaaa catcagttag acaatttgct
    18901 gcattagcag gatcggaccc tattgctgct tctgctttgt taaaaagaac agatgtagat
    18961 ttagtaaaac ctgaaactgt aaaagcattt gaattgggtt atagaggtca atttgaaaaa
    19021 ttctcttatg atttgaatgg gtattataat atttacaatg actttattgg taatttaaat
    19081 gttgttgctc ctttatatgg aactgctcaa gatgctccgg atcctactgc aggaccaaca
    19141 aacgcaggaa cacaatcgct tcatgcctta caaacaggaa acattagagc ttatcaacta
    19201 tacaccaata cggatattga aattatatca ttaggatttg gtgcgggtgt ttcttataaa
    19261 atttacaaag attttgaaat aggagccaat tataattatg ctgaatttga ctttgatcaa
    19321 gaaaaagacc caagttttga agccggattt aatactccaa aacacagagt taaaggatcg
    19381 ttgagcaacg ataaattatt ccctaatttc ggatttaatg taagtgccag atggaattct
    19441 gaatatttat ggcaatctac tatggttgac ggtatgatta gtgaagctac agtaattgat
    19501 gctcaaatta attacggcat tcctaaatta aaatcagttt taaaagttgg tgccactaat
    19561 attggaggaa aagaatacca acaagtatta ggagcaggag caatcggaca acagtttttt
    19621 gttggtataa ccatcaatca ataatataat aacataattt ttaatactat gataaaaaat
    19681 ataaaatggc tattcctggt ttctttaacc attgtagctt gtagcgatga tgaaactgtc
    19741 gttgtaaata atacttcgga tgggttgccc ttaactgctg gttctgctga tttttctaac
    19801 tatgtttctt taggaaattc gttgacagcc ggatattgtg acaatgctct ttttattgaa
    19861 ggccaagcta attcgtatcc tgccatctta gcagatcaat ttgctttagt gggtggtgga
    19921 gaatttaaaa ttccttacac taatgataac acaggaggtt tattgttagg tggaaatgta
    19981 attgccggac ctagacttta ttttaatggt gccggacctg ctccggtaac cggtattcca
    20041 acaacggagg tttctactgt tttagcagga ccatttaata atatgggtgt tccgggagca
    20101 aaaagtttcc atttattggc ccctggctat ggtaatgttg ccggtgtagc cactggtcaa
    20161 gcgaatcctt attttgttcg ttttgcttcg tctccttcaa ccactatttt ggctgatgca
    20221 gctgctcaag accctacttt cttctcttta tggattggaa ataatgatgt gcttggatat
    20281 gccacttcag gaggttccgg aacaaatcaa acaggaaatt ttaaccctgc tacgtatggc
    20341 cctaatgata tcacagaccc aaatgtattt gctaacgttt atactgcttt agttgacgga
    20401 atgaccgcca atggtgcaca aggggtggtt gctaatttac cttatgtaac gacgatacct
    20461 ttctttacta cggttcctca taatcctgtt ccgcttaatg ctgcaacggc agctcaatta
    20521 aatgccggct tttcggctta taataacggc ttattatttg ctcaatctgt tggattatta
    20581 actgctgacg aagtaactag aagaacgatt tctttcacgg cttccactac aaacagagta
    20641 gttatggttg atagttattt aactaatttg gctgcatatg gaattccatc atacagacaa
    20701 gcaacttcag aggatttagt agttttaccg gcaagaagtt ttattggaac tgctgttggg
    20761 ggtaatcctg cacaaatcaa tggcgtttct gtgcctttag cagataattg gattctttct
    20821 aaagatgaaa ttcttgaagt aaaaacggct acagatgctt ataatgcaac tattcaatcc
    20881 attgcaagtg ctaaagggtt ggcttttgtt gatgcaaatg ccttaatgac tcaagtagcg
    20941 aatggcggta ttacagctga taattttact gtaacatcaa cctatgtaac cggaggaggt
    21001 ttctctttag atggtgtaca tcctagtcct agaggatatg ctttgatttc aaatgaattc
    21061 ataaaagcaa ttaatgcaac ttacggttct aactttaaag gagttagttt aggaagttac
    21121 agaatattat atcctgaaaa cccggataat ttttaatttg tcctacaaac tatatcaaaa
    21181 ccaccttagc aacaaggtgg tttttttatt ttactacact atttacaagc atttcatcag
    21241 cttgcttcga atacttcaaa aaatgataaa aaaaacatcg ttttgctatt gattttgtat
    21301 ttttaaaaat tatctttgca ctctgaaaaa agatatgttt tttataatct taaatagcta
    21361 ttaaataaat acataagcaa tgtcaaaagt aataggaaaa gttgcccaaa ttatcggacc
    21421 ggttgtagac gtagtgtttg actccaaaat tgccgaatta cctaaaatct atgattcatt
    21481 agaaatcaaa agaaaagatg gtgctatctt agtgttggaa gttcaatcac acattggtga
    21541 ggataccgta agaaccattt caatggactc tacggatggt ttaagtagag gtactgaagt
    21601 agaagctacc ggtgctccaa tccaaatgcc tatcgggccg gatgtttatg gaagactttt
    21661 taatgtaatt ggagatgcca ttgatggttt aggaaacttg ccaaaaggag gagcaaacgg
    21721 aatgtctatt cacagaccgg ctccaagatt tgaccaatta tcaacttcaa cagaagtttt
    21781 atttaccgga attaaagtaa tcgatcttat tgagccttat gcaaaaggag gtaaaattgg
    21841 attatttggt ggtgccggtg ttggtaaaac agtattgatt caagaattga ttaataatat
    21901 cgctaaaggt cacggtggtc tttctgtatt cgcaggagtt ggagaaagaa cgcgtgaagg
    21961 aaacgattta cttcgtgaga tgttagagtc aggaattata aaatatggag aagaattcat
    22021 gcactctatg gaaaatggag gatgggattt atctaaagta gacaaaccgg gaatgagaga
    22081 gtctaaagct actttcgtat tcggacaaat gaacgaacca ccgggagctc gtgctcgtgt
    22141 agccttatcc ggactttcta ttgcggagta tttccgtgat ggagcaggtt ctgaccaagg
    22201 gaaagatgtc ttgttcttcg tggataatat cttccgtttc acccaagcag gttctgaggt
    22261 atcggctctt ttaggtcgta tgccatctgc ggtaggttac caaccaacat tggcaacgga
    22321 gatgggggcc atgcaagagc gtattacttc aaccaacaaa ggttctatta catcggtaca
    22381 agcggtttac gttcctgcgg atgacttaac tgacccggca ccggcaacaa cgtttgctca
    22441 cttagatgca actacggtat tgtctcgtaa aattgctgag ttaggtattt atccagcggt
    22501 agatccattg gattctacgt caagaatttt gacagctcaa atcttagggg atgagcatta
    22561 taattgtgct caacgcgtga aagaaatact tcaaaaatac aaacaacttc aagatattat
    22621 cgccatcctt ggtatggaag aattatcaga agatgataaa ttagctgttt caagagcacg
    22681 tcgtgtacaa cgtttcttgt cacaaccctt ccacgtagca gagcaattta ccggtatccc
    22741 aggagtttta gttgatatta aagaaaccat caaaggattt aatatgatta tggatggtga
    22801 attagatcac ttaccggaag ccgcattcaa cttgaaaggt accattgaag aagctattga
    22861 agccggagag aaaatgttag cggaagcgta aaacaatgga taattgacaa tggataattg
    22921 tcaattgtta atcatcaatt atcaattaaa ttatgatttt agaaatagta tcaccggaag
    22981 ctaccttatt caaaggcgaa atcacttcaa ttaccttacc cggagttgat ggttcgtttc
    23041 agatattgaa taatcacgcc cctatcgttt ctattttaaa acaaggaacc gttaaaataa
    23101 cagctcctag ttttaaaatc aataaagaag tggcagataa atttaccaaa gtaaatgatc
    23161 aaaactacac gattgaagtt acgtccggga ccattgaaat gaaaaataat aaagtaattg
    23221 tattagcaga ctaatacttt taaaaaatac tgatagaaag cctcaccatc gtggggcttt
    23281 tttaatttta ggagtcgtta ttacagtata ggccagttgt aatttactta tcactatttc
    23341 tatttcctct tcattcaaat gaccgaatcc taaccgaata gcacaggttt ttttatcttg
    23401 ataaagaatg gttttaggta aaaataaatc taattttgag gcttcttcgg ccagttgaag
    23461 aagcgaaatc gaaggttgaa atgtaatcca aatggctaaa cctccagtcg gttttttaaa
    23521 atataccgta tcattaaaat acttattcag acattcacaa agataatcac gtcttcgctt
    23581 ataggtaatg atgttttttt taagcaaacg atgaatttcc ccttcctgaa ttaattctga
    23641 aagcatttgt tcctgaatca aatctccttg tcggtctacc atttgtaaat agtttttggc
    23701 ttcagcaatt aaattctcag gagccacaac aaatccggtt tgaaaacttg gtaaaaaaga
    23761 ttgccccagt ttccccaaat agataatcat tccatttgca tctgcactcg ccataggtaa
    23821 cattgctgtt ccttccaatt gaaaatcata atcataatca tcttcgataa tggcaaattg
    23881 ataggctttt gccagttcca ttaatttaat gcgtcgctca gctgacaggg aataggtagt
    23941 tggataatct ctatttgaac aaatataaac acaacggatt tttcctttag taaagtgttt
    24001 ttcgatataa tcaatatcta aaccgttttc atctatcgga atggtaatga ttgtagctcc
    24061 ggcctcttga aaaatcatat tcgcattatg attacttaat tggccaacta aaacaatttc
    24121 tttttttcga atcaccaatt gcgaaataat atacaaactt aattctgtac ttctggtgct
    24181 gatgacgttt tgaggctgca catgaaaacc gcgagaagca tttaaataat tgcacaattg
    24241 attttcaaaa gcagaattct ttgattttga attggggttc cattttgaaa tcaatgattt
    24301 tcttttcatg gaagcactat accattttga aaattcgtaa acaggatgta acctcaaatc
    24361 gggttgtccg tcatttaaac tatattttgc ttctgaatat tcattggtgg aagacaaaag
    24421 gaaagaagtt tcaaagggaa acccagtgtg tttaggataa tgatgcacat ctccaatttt
    24481 attggattga gctttaagag ctgtcttttt gggcttaggg tctaaaataa atgttccttt
    24541 gttagcaatg atttcaaccc agccttggga agctaattca tcataaacgg ctactgccgt
    24601 atttctgtga atttggaata attctttaaa cgttctggtt cccggtaacg ctgttccggc
    24661 acttaaatag cctctttgaa tggcattgat aatttgttgg gctatttgaa tatatactgc
    24721 tgtgttgctc gatttgtcaa attgaatcaa ggctgttagg agggaattaa ccggactatc
    24781 tttcatttta atagtggact agtttaatcg tccggaaagt tactactttt gcctcgtttt
    24841 taactcattt aacatttcaa aaaatgaaaa gaagaaattt tcttgttctc tttttagtgt
    24901 ttacttttat agtaactgct caagagaata gccaacgaat tgatacatta agtgaagtta
    24961 tcgtgtcttc tacccgaatt gatattccgt tttctcagaa ttcgaggacg attcaattga
    25021 ttacctcgga acaaatcaag caaagtggtg ttactaatgt ggcggattta ttattgctaa
    25081 tatccggtgt tgatgtgcga agaagaggaa ccagcggtat gcaagctgat ttgtacttaa
    25141 gaggcggaag ctttgaccaa acgttgttgt tgatagacgg cataaaagtg gaagatgctc
    25201 aaaccggaca tcacacgatg aatttggctc taccgataga agtgattgag cgaattgaaa
    25261 ttatcaaagg gccggcagcc agagtatttg gtcaaaatgc ttttaccgga gcggtgaata
    25321 ttgtgacaaa aaaggcatta tccaataagt ttaccctgca aggtcaagcg ggttcgtatg
    25381 aacaactaaa tttatcggcg accattatgc aggattatga gaaaacttcc catgtggtac
    25441 agttttcaag aaacatttca gagggttacc gctataatac ggattatgat aaccagaatt
    25501 acttcattaa gagtaccatc aaatcaagtg aaatgcctat tgatattttg gccactttct
    25561 ctgagcgaaa atttggagca aatgggtttt atgctactcc ttctgccatt aatcaatatg
    25621 aagaaacaca agccagttta gtaggtgttc aatcagtatt tacaaaaggg aatgtgactt
    25681 ttaaaccgag aatgtattgg cgaagaaacc aagacgaata tgtgtttgtt cgggataatc
    25741 cggaaatcta ccgaaattta catatcacga acaaagtggg gatggaattg aataccgcta
    25801 ttcaatcgaa atggggtact accggtgtgg gagtggattt agcaaaagtg tatttgtcca
    25861 gtaacaattt gggcgacaga gatcgtttta tggggacttt tttcttggag caccgctttt
    25921 tgttgttgga ttctaaatta gatattacac ccggatttgc tgcaaattat tatactgatt
    25981 ttgatggtaa ctttttcccc ggtattgata ttggctacca ggtttccaaa cactttagaa
    26041 cctatgccaa tgttggtaaa acctatcgaa taccaaccta tactgatttg tattacagcg
    26101 acccaaacac cttggggaac gaagatttaa aacaggaaga ggctctttca gaagaaatag
    26161 gggtggtgtt tacgcaaaac aaatttgact ttaccgttgc ttttttcaac agggattcca
    26221 agaatttaat tgattatacg aaagagctgg aatctgataa ataccaagct aataatatcc
    26281 gtagtttgaa taccaaaggg tttgaagtgg ggtctaatta taaatacacg tggttttctt
    26341 ttgctcaacg aatcaatttg ggctacactt ttattgaaga cgatattcag gaaattgatg
    26401 ctgctttttc ccgctattct atcaattctg cgaaccatca atttacgggt caatggaaca
    26461 gtcagttctt tcaaaacatt tcacaaacca tttcctatcg ttatgtggag cgtacgtccg
    26521 gcgagagtta tggcatagta gatgcgaatg tggtggtgcg aatgaaagcc tttgaatttt
    26581 cgttagtggc gaataatatc tttaatgtag cttattccga aactaattta gtgcctatgc
    26641 ccaaagggaa tctgcttttt ggggtgaaat atgactttga ataactgctt tgtattttag
    26701 tataacagaa gagggtcttg gtaaaaggct ctcttttttg cttttaggga taccacgact
    26761 atggctttaa ccaacctttg taatgacgta tttatggata tgaccaactg attgtatgtt
    26821 gtgaatgaat tacatagtcg ttataaagtt actatatagt tactataaag ttactatata
    26881 cttactataa agttactata tattctattt aaaaatcttc tttgattggt gatttcttac
    26941 tgttttggtg attcttgtga aggaggttct tctgaagatt gagttgagga gggatatgaa
    27001 ataattaatt aactcctgat gtgaagcgat ttttgctttt caatgttttt ttgaaggtag
    27061 gcagttcttg aaccggggca tttatttgaa taaaaatatc ccacccggtt aaaggtggga
    27121 tacaaacaca aattagggca taacaaactg ttatttgaaa attatttttt ttgtaataac
    27181 ctgtctattt gcatcggtta cttttacaat taagaacgga tttgccgtgt ttaactgatt
    27241 tgttactacc gcaatatctt tcacgcctgt ttggctccaa attaaggtgc caaatacatc
    27301 atatacttct acggattcta ttgggaaacc ggtgctgttg atgtgaattt gtttttcagc
    27361 agaccaaatt acacaatcat tgttagggag ttgtgttact gtttcggttg aattttgttc
    27421 agtgaaatac aattcaaatc ggttattgaa ggtgccggct ttggtgtcaa aggtatagga
    27481 gccttcattc aagttatggt aactattggt gagtttgtct tttaatacca ttgttttgta
    27541 gtctgaaaag agtccgtcgc tgtgacctaa ggcaagggta tattttcctt cgttggtgac
    27601 tttgaagcct agcggaataa tgtcttgtgg gttaaaaggc aatgctcttc cttgtatggc
    27661 caattgttct tttcctagga tggtatagat atctatctca ttttgagtaa aacttgatcc
    27721 gtcatattgc gtatctaatg cattggttgc tccttcaatg tagcccacca tcatttgacg
    27781 ataggcgtct ttgttgttag agaggttaat ccaaagtcgg tgtttttctg tttcactgtt
    27841 ttctttgacc ggttgtgccg ttctatagaa ttgggtgttg ttgtctttta ttcgcatgct
    27901 gttgttgaag gtgatgtttg catcagctgt tacttgagca aaaaatcctt gaccggaagc
    27961 aatgtagcct tttggagtta agctttcatc ggttgagctt agcggttttg ttccaccact
    28021 aagattccaa gtggcataat ctgctacttg atattggcct gttccggtgt agaccgttac
    28081 ggatgtccaa aacaataatt tcactatttg cggatgttgt aacaataggg cattggcttc
    28141 gatggcacta ggatagggat ttgccaataa tatggagttt ccgctgtcgg tatttacatc
    28201 accgccatca aacacatttg cataaacgct tatcggacct gaatttggca acccaacgaa
    28261 aggaaattcg attgttttgg gtgaagcgtt taccggaaca taggcttgag aaactctagc
    28321 aatgaatcca tttccaaccg gcggtggagt ggtgatgctt acccaagctc cgtcttgagc
    28381 tccggtgagg ttccaacggt attttttgtc aaaagcggtt ggaatagcat agctttcggc
    28441 aatcggtgag ccccaatata catagtctaa tcctcgcatt ggtcgtgaga ttctttttat
    28501 ggtgattgga tttcccgtaa aggtagccgt gttttctttt tgaattaaac taccgctact
    28561 ttccactacc attgccccat tattgacaat gttgttttct acttcaaggt aggtgtttgg
    28621 tgcgatttgc attgtttttc cggcctcaat attcaaactg caagctgaaa aactgccgtt
    28681 tagtgcggta tcataatttg ctgctattct tactttggat aattcggtgg gtggcaccgg
    28741 attccagctg cctgtccagg ttgaaacagc atttggtatt ttcaactcgt tactattggt
    28801 tacataatta ttgaaataat taattgtaca agtgtaagta atgttatata ctccatttaa
    28861 atccaacggc aattcaaaac tatctcccgt tggatagaaa gtattgttca aagggtctac
    28921 taccttccaa gaggtagtta cttcaaaatc gtatcgagaa ccgaggtaac ttccggttat
    28981 tgtggctgaa gtgctgctgc acgtaatggg agaagttgta atgacggagt gaatagaacc
    29041 accgatttcc atcagttctt gaaaagttaa cgcagaactt cctccgccac gacaataggc
    29101 aatgacgaat gttccgatgt agatttgttg aaggtggtta caatcgccta ctaaaccgcc
    29161 cacactcact tgaatggaag cgttttcggg caacatcaat tttactttgt tgtcaaattt
    29221 tattcttcct gccggaatgg ctgtaacaat aatggaaggg gtgttgaatg aaaaagtact
    29281 tccgggactt acgttaattc catttaagtt aagttgtcca cttacggtaa tgattcctgt
    29341 ttgattgagg tatacattat tcgttcccat ggtgagggtg tgtccgtttc gaattaatac
    29401 ggatgctgtg ctggtgtttt gaccgggata ggtggttgca gtgacccaaa ttaatccgtt
    29461 gaaggtttgc caagtagcgg ctgttgtcca agctccggaa attctcgatc tataatcacc
    29521 actgttttgt gcttgcacac tgatgataaa cgtcatcaag agtgctgaaa taagtaattt
    29581 aacacgcatg atttctaaaa tttttaattg tctatctatt aaaaatccca gggatgtttt
    29641 tggacactta aagttacgaa atattgaaaa aaaacggttg agaaaaagtg ttttttttcg
    29701 ttttttagtc tgcttcgtag gagatttctt tttccttttt tgaagttctt ttaaaagtga
    29761 aaaagcgagt cgtttttagc tgtttttttc cagttttttg acggcttcat acaccaactc
    29821 actttcccat cctttgcgaa gcagggtgtc gcagaatttc ttgcgttttt tgagggcgtt
    29881 tttctcagtg atggtctgcc aggttttttc ggttagggat tcaaaggttt cttcgtattc
    29941 gtgggatgga atttcctgaa ggcctttggt gatgaggtag gtagaaattt gtcgttgttt
    30001 gagttcgtta gtgattcgga tttttcccca ctttttttga tgaaatttac tgatagcaaa
    30061 ggttttggca aaccgttctt cgttcagaaa attatgctga attaaatgca ccacgacttc
    30121 gtcccgctcc tgttgtgtga gggcaaaacc atagagtttt tgcatcactt cgaggtggca
    30181 acgctcttga taggaacagt agtattccat cttttgaacg atttgaggca gggaagtgct
    30241 tgatactgag gccaccgttt tgtaaat
//
LOCUS       DAOTCW010000010        29979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_776_length_29979_cov_42.167157, whole genome shotgun sequence.
ACCESSION   DAOTCW010000010 DAOTCW010000000
VERSION     DAOTCW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 29979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29979
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_776_length_29979_cov_42.167157"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="P5335_01645"
     CDS             complement(<1..692)
                     /locus_tag="P5335_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRZ73612.1"
                     /translation="MNYFSSDFKLGILGGGQLGKMLLAETRKFDIQTYVLDPSKDAPC
                     QFGAHYFEIGNLLDFDTVYNFGTKVDVLTIEIENVNLDALDKLEAEGKKVFPSPKTLR
                     NIQSKITQKKLYLQYQIPTANAKPFDDKKDLLVEVSQGNLIFPCVWKQDRFGYDGYGV
                     KILKSENDLVDLPETPCLIEEMIPFKNELAVIVTRSVSGEVKTYPVVEMEFHPEANQV
                     EYVICPARIDEKV"
     gene            complement(794..1339)
                     /locus_tag="P5335_01650"
     CDS             complement(794..1339)
                     /locus_tag="P5335_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73613.1"
                     /translation="MNSTLYQKIEQSTAHVKSRTTVSNFAMKSEENLSELISIAFDIN
                     HDLHVKAFWSLDLIGEKKLKLLVPYLDDFCGVLPHIKEDSSLRPATRIAMFLVKSNHR
                     KNGINLTKEQEHLLIEAMIDRLIQDVKVASKVYAMKALFILGKKYPWIYEELKPILSQ
                     DYSNQSAAYKAAARNILKRLK"
     gene            1435..1965
                     /gene="hpt"
                     /locus_tag="P5335_01655"
     CDS             1435..1965
                     /gene="hpt"
                     /locus_tag="P5335_01655"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRZ73614.1"
                     /translation="MIHLHDKTFEPFISSEEIDFAVSNMAKQMDDDFFDDIPVFIGVL
                     NGAFMVLSDLMKQYRGMCEVSFLKLASYEGTQSTNEVKQLIGLNQNLEGRTVVIVEDI
                     VDTGNTIEELKVIFKEHNVKHLKIATLFLKPDAYKKEIKIDYVGIRIPNKFIVGYGLD
                     YDGLGRNLPDVYQLAE"
     gene            2002..2577
                     /locus_tag="P5335_01660"
     CDS             2002..2577
                     /locus_tag="P5335_01660"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRZ73615.1"
                     /translation="MINIVLFGKPGAGKGTQAEFLKEKYNLTHLSTGDIFRFNIKNDT
                     ELGKLAKTFMDKGDLVPDEVTIQMLQSEVDKNPNSAGFLFDGFPRTIAQAEALDSFLV
                     SKKQEITATVALEADDNILVQRLLERGKTSGRPDDQDEEKIRNRYQEYNEKTAPLMEY
                     YKNQGKFHAVNGIGSILEVNERLNFVFDNLK"
     assembly_gap    2765..2864
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2903..4126
                     /locus_tag="P5335_01665"
     CDS             2903..4126
                     /locus_tag="P5335_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRZ73616.1"
                     /translation="MSEIALISARKSRLETAAKKGNPAAKVALDLANSPNKFLSTVQI
                     GITLIGILTGIYSGDKITTDVKNFVSGFEMLKPFADSIAVGIVVVVLTFFSLVLGELV
                     PKRIGLNYPETIAKAVAVPMKIVSKITAPFIWLLTISTEFILDLFRIKPTADGKVTEE
                     EIKAIIKEGTEVGEVQEIEQDIVERVFHIGDRKVNSLMTHRSSVVYLSLEDTLQELKA
                     KVLEELHSFYPVCGENMDDIEGIVLLKDLFSSFEKGSFDLKTITKEPVYFIEHTSAYK
                     ALENFKTSKVHYALIVDEHGVFQGIITLNDILEALVGDASDFYEEEFKLVAREDGSWL
                     VDGHYSLHDFLTYFDMDENVNDYDVTTVSGFIITELGNIPKQGQKLIWNKLEFEVIDM
                     DGVKIDKILIKNLKE"
     gene            4212..5216
                     /gene="obgE"
                     /locus_tag="P5335_01670"
     CDS             4212..5216
                     /gene="obgE"
                     /locus_tag="P5335_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRZ73617.1"
                     /translation="MTEGNFVDYVKIYVSSGKGGKGSSHLHREKFIEKGGPDGGDGGR
                     GGHVYLKGNKSLWTLFHLKFVKHIRAGHGGDGGSSRSTGHDGEDKFIEVPLGTVVKNQ
                     ETGEVLFEITEHDEVKIIAKGGKGGLGNWNFRSSTNQTPRYAQPGMPVEEADVILELK
                     VLADVGLVGFPNAGKSTLLSVLTSAKPKIADYAFTTLKPNLGIVAYREFQSFVMADIP
                     GIIEGAAEGKGLGHYFLRHIERNSILLFLVPADAADIKKEYEILLDELRRYNPEMLDK
                     DRILVVSKCDMLDEELKAELEKQMKKEFKGVKYHFISSVAQQGLQELKDMLWKMLNAE
                     "
     gene            5384..7531
                     /locus_tag="P5335_01675"
     CDS             5384..7531
                     /locus_tag="P5335_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRZ73618.1"
                     /translation="MKKIILSFVALIAFVPATVFANEGMWFLMHIQRLNHRDMQKMGL
                     QLTAEEIYSINNSSLKDAIVQFNGGCTAEMISKDGLVLTNHHCGYDAIAELSTTESNY
                     LRDGFWAQNRKAELKPKSLYVRFFVRMDDVTKRMLAVVNDKMTEAEREAAINQETAKI
                     QKENDGGGKYTVSVRPFFQGNEYYYFVYQDYKDVRLVGTPTESIGKFGGDTDNWEWPR
                     HTGDFSMFRVYADANGNPAEYSENNVPLNPKHHLPISLKGVKENDYAMILGYPGRTNR
                     WMPAEGIEQNVKYAYPAWVEGSKMGMDKMKVHMDKDAAVRLMYASKYAGVANYWKNRQ
                     GMIDALSKFKTADAKRKNEAKFNAWANKPANKAKYGEVVPTINKYYAMTNEKARHDNY
                     LQQLLRTSAFGTISRGLGKQLEGYAKADVAKRAQLKPGLQEMIDGFFGEVYLPAEKDI
                     LAGQLALYNAKAGYKIAPSVEEMAKANNNDFTAYVNAAFELSMFTSKEKLNAFLEIPS
                     EAMITNDPLNKLSNDMLTHLTTRSDELTQAQNDYSKSFRLLVEGLRESKLSPIQYPDA
                     NSTLRLTYGKVRALPADKRNDAKINNYTTLEGMVKKHKAGDEEFNLSQPLIDLYNKKD
                     FGQYADKDGSLHVNFLTDNDITGGNSGSPVLNGKGELIGLAFDGNIEAMAGDVIFDDK
                     LQRTINVDIRFVLFIIEKYAGAKHIIDELTIVK"
     gene            complement(7610..14134)
                     /locus_tag="P5335_01680"
     CDS             complement(7610..14134)
                     /locus_tag="P5335_01680"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRZ73619.1"
                     /translation="MYKTITICCILIASYSFGQNFDEQWKEVYKLELDGKIQSAQKEV
                     QEIYKKAKRKKEEVQIIKCFFYVSKFEQVLNEKAQSTIISTLKEEIKEAKPASKALLN
                     YIYATLLQNYYNQNSYKIGKRNDLENQKSTDFSTWSTSDYIDEIEKAMENSLQNEKSL
                     RATTINDYKEIFEVSPSIDAKNYSLYDFLVEKNMAHYKSKIKNFDQKNKSDLFQNLYA
                     ESTIFVLYNLTLLVDDNLIKLLKILQNNERYYLSEKKEKADFAYFERLKYFHSLYSEY
                     SVYPEKIKQLENITSNSFLKQSLRIERIKNYISQTSKNNKINKYKEALALIDTVLNTK
                     INPNTLAEAETLQNQILQKSLSVRLQTTTYPNQNNRAFLDYKNVDSIKISYYLFPVKN
                     NYLLTNSYNYDTNYKKNKDSLIINYVQQNKPIKSFTRKLTSSNDFFNHSTEIILEKID
                     VGNYLVFLETTNDTIEKKIAFTYENFQVSNFFIVEDKNEKYDLIYVLDKKTGKPIENV
                     SIKNEDETDYTNKEGKAQFRLRKQIQNKIYNNDILIIKENDTLLKKYHRTFIYEREEN
                     NEDDFDNFEAKAMVYFDRAIYRPGQKIHYKGILIQNKDNIKSVVPYVSIHVEINDVNE
                     NILKEFDVQTNEFGSFSGEFDIPKNTLTGNFYITIDEADNYEMDAKYYDSNEEEHKFW
                     DNVDFDVDNFYFKVEEYKRPTFEIIFDEIKENYTISDTIKIKGKAKALAGNNLTNAKV
                     AYTVSKKTTSNKSSFSDDDDFIIAETATDENGNFNIQFPAIEEGVSNDSILLIRYTVK
                     IDVTDIQGETRSAFKYIKVAKEMLELNVLLNSNLFKEDDNKIIIKSTTLNNYTIDSKG
                     EIKIYELQKSIFLKNRLFGIPEIQTLSRNEFEQLFPHEPFDQSDYETKEVLVKTISFD
                     TKTSNEISFDFLKSFKNGYYKVVTEAYDKKNNLIKTEKNFNLDSKVSPNTKDELFTFK
                     DTSKPNGNLFEIEILSPIPDLWITSRFYGENNTLENEQVVQLKNGKVILKFNKKSEYK
                     TDVHFYFSSVWENNTAVKTHSIQKETIEPKLNFEIVSMRNKIEPGSLENWSFKIIDQK
                     LESEVLASMYDRSLDQFDTQNWSTVNFSDSSDYTNFPDLKFANISYKTFNNFKMDRKY
                     YSYYIQNPQINWFGFNYTYPKNSQTQKKYLDKIKPLTELPNNAKNITGVILDDSGMPL
                     PGASVIIKNTNRGTSTDFDGQFNIEAAPGEVLFIGFVGYNSIEIIIGNKKDYYINLKD
                     SENYLEEVVVTGAYGIKKKEKSVTYASQSMNSNTLAEMNATDLITALTGEISGVQVTN
                     SSGVPGSSTRIILRGASSIKGDNQPLYVIDGVPFEGDSSTLDLENIKEISVLKGPSAA
                     ALYGLRGANGVVIITTKNAFKELTQVKTRTNFNETAFFYPHLKTDSDGKFSFNFNTPE
                     SLTTWKLRLYGHNKKTESGYFESTIISQKDLMVQTNMPRFVREKDSISISAKVVNMTN
                     DTKSGIAMLMLFDATNMKAIDSIALNPNNIRNFDCKPKESVPVTWKITIPEGLQGLQY
                     KIVAKSGNYSDGEENILPVMSNKILITESIPIWVKGNSKKEYVFENLKNNSSSTLKNH
                     QFTLEYTSNPTWLALQSLPYLMEFQHECAEQTFSRYYGNFIATEIINSNPKIATLFES
                     WKTNSTSVSKLNQNEELKSIVLNETPWLLDAESEELKNNRLALLMDLNTMKESQENTF
                     KKLEEKQLSSGAFPWFDGGDENMFITQHIIAGLGHLGKLFPESISKFEKITSKAIPHL
                     DNNFIKTSTLKNERINYYTYSNLHYLYARSFYIEKLPVSKKTDSIITIQKVEFKANWL
                     QYSLYKKALLALTMYRLGDKDFAKNIIANLKETVAINDDFGMYWIENKNGYYWHQSSI
                     ETQALLIEAFAEIEKDKKYVDEMKVWLLKQKQLQNWPTTKATTEAVYALLLQGNDWIN
                     IKDNTKFKIGNEKVLTKKLIEKDKEAETGYIKMNWNSDEITKEMGLISVENKSSVAGF
                     GGLYWQYFENLENIKSDSTAILSVTKNVYKKVKSSFGNQLVELNKESLKQGDLITIRL
                     IIKTENDLEFVHLKDLRASCFEPVDVISKYELKDGLRFYRSTKDVATHFFFDTIKRGT
                     YVLEYDVRVNHSGSFIDGISTLQSMYAPEFSAHSISTKVKID"
     gene            14278..14964
                     /locus_tag="P5335_01685"
     CDS             14278..14964
                     /locus_tag="P5335_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73620.1"
                     /translation="MQIFKVVILLFSLQLMANSDFDKAEKLFQQNKLAEAQLLFEKYL
                     KENPTHTKTLEYLGDIAGANKNWDKAIVYYQKIKNQYPQNAVYQYKYGGVLGMKAKEV
                     NKFKALGMLGDVENAFLNAAKLDPKFIDARWALVILYIELPGIVGGSETKAQKYADEL
                     MKLSKVDGFMAKGYIDEYFNRYSKAESNYKKAHEIGNSKTTFQKLYDLYQKKMKQPQK
                     AAELKKQFEK"
     gene            15030..16385
                     /locus_tag="P5335_01690"
     CDS             15030..16385
                     /locus_tag="P5335_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRZ73621.1"
                     /translation="MRTHFIAIGGAAMHNLALALHHKGYLVTGSDDAIFEPSKSRLQK
                     HGLLPEEMGWFPEKITSNIEAIILGMHAKADNPELLRAKELGLKIYSYPEFLYEQSKN
                     KTRVVIGGSHGKTTITSMILHVMHYHNIEVDYMVGAQLEGFDTMVHLTEKNDFIVLEG
                     DEYLSSPIDRRPKFHLYQPNIALLSGIAWDHINVFPTFENYVEQFEIFVNQITKGGIL
                     VYNEEDETVKKVAEETTNTIRKLPYQTPTYSVENGTTYLDTSEGPMPIEVFGAHNLNN
                     LAGAKWICQNMGVDEADFYEAIASFKGASKRLEKIAEGKGKVAYKDFAHSPSKVSATT
                     KAVKNQYPDRKLVACLELHTYSSLNAEFLKEYEGALDAADIAVVFYSPDAVKIKQLEE
                     VTYDQIAQSFKRNDLIIYTNPTEFKDFLFSTNLDNSALLLMSSGNYGGLDFVEVKNLI
                     N"
     gene            complement(16460..19540)
                     /locus_tag="P5335_01695"
     CDS             complement(16460..19540)
                     /locus_tag="P5335_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73622.1"
                     /translation="MKKLNTIIYLFGLFTVCFSQAQEEVILKGKELYGDIKARQIGPA
                     IMSGRISDIANHPTNNKIIYIGAAGGGVWKSSDGGASFSPIFDKHIQSIGCVTIDPNN
                     PDNTIWVGTGEPWTRNSVSIGDGIYKSTDGGLNWNNMGLPKSDRITSIVVDPRNSDVV
                     WAGVLGALWGDSEDRGIYKTTDGGKTWNKVFYVDQKTGCADLVIDSKNPDIMYASFWE
                     FRRTAWSFNSGGNNSALYKTIDGGKTWNKIHTGFPKGQLGRIAIATAPSNPEILYAVL
                     ETEQDKDKGLYRSDDSGATWKHLNGDFELVVRPFYFSRITVDPRNPDIIYKGGLRGSI
                     SRDGGKTFKGLGNMHSDIHDITIDLVDSNRIYVATDGGLYRSWDAGNTMDIVKNLPLS
                     QYYHLSLDNEEPYNIYGGLQDNGSWYGPSKSDGGIEARDWHSVGYGDGFRVLKHPTKK
                     IIYSEMQGAENIWRYNLDTKELKTIQPLATKGDPKLRFNWNTPIVTGIHNPDRIYAGS
                     QFVHVSDDMGQTWRKISGDLTTNDPSKTNTEKSGGLSVDNSGAENHCTIFTITESPLN
                     KEVLWAGTDDGNVQITKDGGKTWSNITLNLKGLPKNTWCYHIEASVFDASTAYAVFDG
                     HSKNDYATYVYKTSDFGKTWNSIVTADLDGFARNIQEDFKNPNLLFLGTEKGLYITID
                     GGKNWSKFENEMPAVAVHYIDLHPKTNDLVMATHGRGIIILDDISPLRQVNQHILKKE
                     VHFFDTKSFVIEEQSSFGGTASELEFVGPNPNTSAQLVYYLKKRNTFGKMDLEIQDMK
                     GNKIITLAPGNQKGINIVTWGFNTKNPKLATAKTLSYGGFTAPRVPAGTYKAVLTKGK
                     ETYVKEFQVVNDPKSVISEAERNKQAETTKMLFDKNEELAYMVYELDEMISLNKKIME
                     KDPKSAKTNGKIDSELNSLKNTMVVTTGDMYVGAAEPQLREKLSTIYATVASQFDAPS
                     PSQVSNIENVMDKFNTAASGFKDLKGKYKSKLMEQAAKLEIPFTLKTFEEFLKD"
     gene            complement(19605..20339)
                     /locus_tag="P5335_01700"
     CDS             complement(19605..20339)
                     /locus_tag="P5335_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRZ73623.1"
                     /translation="MKKIGSLVSMMLLVFVSFSTRAQTADEIISTYLKNIGGVEKFKS
                     LTGTKTEMTVNYQGMEIPVELVQLKGGKMYLKINFQGQEITQMAFDGTTAWSTNFMTM
                     KAEKLESEMVQNIKDEANDFPDAMLDYKAKGYTAEYLGKETKEGTECHKIKLTKKPQT
                     IDGQKVDNVVFYYFDTENNVPILTETEVKSGPAKGQISTTTMSDYQEVDGIFFPFSMN
                     QGGQAMKIKKITLNPAVDQSAFAFKE"
     gene            20451..21146
                     /gene="radC"
                     /locus_tag="P5335_01705"
     CDS             20451..21146
                     /gene="radC"
                     /locus_tag="P5335_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRZ73624.1"
                     /translation="MEDTTSFSIKYWAEDDKPREKLMLKGKSTLSDAELLAILIGSGS
                     RNESAVELSKKILASVQNNLNSLGKMSIQQLTKFKGIGKAKAISIIAAGELGKRRKEE
                     GVQELIKITSSRIVYEIMQPIIGELPHEEFWVLYLNNANKIIYKAQLSKGGITGTVVD
                     VRLLLKTALEHNALSFVLTHNHPSGKLIASEADIQITQKLKLAAKQLDMSVIDHIIVT
                     ENGYFSFADEGIL"
     gene            21161..21721
                     /locus_tag="P5335_01710"
     CDS             21161..21721
                     /locus_tag="P5335_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HRZ73625.1"
                     /translation="MSEKRKSQAKLIRIFRKVHRTTGALLFVFFFLISVTGFLLGIKK
                     HSGGVILPETKIGSTTQLTKFLPLDSLQKNASRFLADYLNQTKKIEIDRIDLRPEKGI
                     VKFTFVSNFYEVQLDGSTGSLLQIDLRRSDVIEKLHDGSIVDYYLGFESGVFKLIYTI
                     IMSLALMVFTITGFWLWYGPKQMRKS"
     gene            21796..22425
                     /locus_tag="P5335_01715"
     CDS             21796..22425
                     /locus_tag="P5335_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRZ73626.1"
                     /translation="MITIKNVFFSYNSTTAFHFPDMNCSASEALLITGNSGKGKTTLL
                     HLLGGLLRPKSGEISIENNNITLFSEQELDQFRGKNIGLVLQQSHFIASLNVLENVVL
                     ASWLATGKRATEKAKALLKELDLEDQMYKLTAELSVGQQQRVSIARALINEPKLLLAD
                     EPTSSLDDENAAKVADLLAQLSKEYKASLIIVTHDHRLKEKFSNHININ"
     gene            22427..>23243
                     /locus_tag="P5335_01720"
     CDS             22427..>23243
                     /locus_tag="P5335_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRZ73627.1"
                     /translation="MIGKLAWKNLWFKPLNTVLSLVLLTASVAIISLLILLQEQFEKQ
                     FSSNIDGVDLVLGAQGSPLQLILSSVYQVDAPTGNINYNEAEVWMKHPFVETAIPLAF
                     GDNYRGFKIVGTTPDYIKKYTATISEGKVFEKNFEVVIGCEIAKKLKIKVGDKFFGTH
                     GDAQEGDVHDEFAYVVTGIASPTGKVIDNLILCTVESVWEMHSGHDHGGEAHSEDENP
                     KHGEEGHIHVDGDEQDHEEHVHDENCNHDQEISENPAHGEEGHVHEEGDDHEHA"
     assembly_gap    23244..23343
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23344..23634
                     /locus_tag="P5335_01725"
     CDS             <23344..23634
                     /locus_tag="P5335_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRZ73628.1"
                     /translation="RVSGASRLQLLALVLFESLLLCIVGFVFGTLIGRIALMMISATT
                     DEQFKMAFNPMIILWEKEGLLFLVTIFVGVIAAVIPAIKAYRLNISKTLANA"
     gene            23627..24184
                     /locus_tag="P5335_01730"
     CDS             23627..24184
                     /locus_tag="P5335_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73629.1"
                     /translation="MRKIYIFFIIIFGFIIFLDSSFIPKIGVNQIDTKTVKPSLQTSS
                     FMPELIENDAVFMQVPDTLSWKLLGVIKFIKKPDKDYPDGVMFPIVNSTLKEKNKKKI
                     VMSGFIIPIDNVSYALSKNVFASCFFCGQAGPETIMGIKFKGATPKLKTDQYVTMEGT
                     FRYNDNDVNDWIYHIENAVIIKGGK"
     gene            24196..24882
                     /locus_tag="P5335_01735"
     CDS             24196..24882
                     /locus_tag="P5335_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HRZ73630.1"
                     /translation="MFKNKTDIFFDLDHTLWDFEKNSALAFEKIFEKHQLDLNLEEFL
                     LHYIPTNLKYWKLYRDEKISQEDLRYHRLKEVFDLMNKEVVDEMIHLLSHEYIHYLPQ
                     FNYLYEGAWEILDYLFPKYKLHIITNGFQTVQAGKLKNSNIEHFFQTITNSEMAGVKK
                     PNPLIFEYALKKANATKSKSIMIGDSLEADIEGAINVGLDAIFFNEFNVSVDEKIHQV
                     NHLMLLKEYL"
     gene            24899..25639
                     /locus_tag="P5335_01740"
     CDS             24899..25639
                     /locus_tag="P5335_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73631.1"
                     /translation="MKKIIFAFLILLSNSTFSQTLNDYQYVIVPVRFDFMKSDNEYRL
                     NTFTKYNLEKMGFKAFYNNTEIPKEIVANACEVLKVEVEKSGSFIWTNLTIVFKDCQN
                     NIVHKSAVGKSKEKDFNKAYTEALNLAFASLYELNYSYNGATKSLNIPANSTVIASEV
                     IETSEVITKPQTTTENSLQALKINGGYHLVDTAQKVVFKLTSTGKADFFIAQKGNHSG
                     VVFQKEGQWFFEYNQEDKLISEKLEINF"
     gene            complement(25636..26913)
                     /locus_tag="P5335_01745"
     CDS             complement(25636..26913)
                     /locus_tag="P5335_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRZ73632.1"
                     /translation="MEAPLAERIRPQHLSEYISQLHLVGEHGSLTHQIAKGIIPSLIL
                     WGPPGTGKTTLAQIMAQESKRPFYTLSAINSGVKDIREVIEKAKQSGGLFTAKNPILF
                     IDEIHRFSKSQQDSLLAAVEKGWVTLIGATTENPSFEVIPALLSRCQVYILNSFSKAD
                     LEQLLQRAIENDVVLKSKNIQLIETEALLRLSGGDGRKLLNIFELIVNATDIEPIEIT
                     NEKVMQLVQKNTVLYDKTGEQHYDIVSAFIKSIRGSDPNGAVYWLARMIEGGEDVKFI
                     ARRMLILASEDIGNANPTALIMANNTFQAVTTIGYPESRILLSQCAIYLATSAKSNAS
                     YLAIGKAQQLVKQTGDLPVPLHLRNAPTKLMKELGYGNEYKYAHDFENNFAEQEFLPD
                     ELQNNVLYEPGNNPREKELRTFLKNRWKDKYGY"
     gene            26987..27745
                     /locus_tag="P5335_01750"
     CDS             26987..27745
                     /locus_tag="P5335_01750"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRZ73633.1"
                     /translation="MKQKPFLFSTSVIAWPLFFVLAMWMVFWVEVRFKVDFGDYGILP
                     RSWSGLRGILLSPFLHGDIKHLYNNSIPLLFLIALLRYFYREVSLQVLIFGILVSGLG
                     TWIIGRENYHIGASGLIYVLVSFIFFKGVQTKYYRLVALSLVIVMLYGGTIWYIFPEV
                     DNQISWEGHLAGFITGFLFSILYKAEKYKKPIRYEWEKPNFNPELDPFMKHFDENGNF
                     VNTPKEEELEYIETQNQFTPSSNIVYDYIPKKEE"
     gene            complement(27747..28481)
                     /gene="rlmB"
                     /locus_tag="P5335_01755"
     CDS             complement(27747..28481)
                     /gene="rlmB"
                     /locus_tag="P5335_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRZ73634.1"
                     /translation="MEKENQIFGIRAIIEAIQAGKELDKVFLQKDAQGDLMRDLLKTM
                     KQHKVNFTYVPVEKLNRLTSKNHQGAVATIAPVSFHDLETLVTTVLESGKTPLFLILD
                     QLSDARNFGAIIRTAECTGVDGIIIGKQGAAPVNGDTVKTSAGAVFNVPICKVEHIKD
                     AIFFLQGSGVKTIAATEKTDSTLYDLSLNEPLAIIMGSEDRGVNPSVLKIVDEKAKLP
                     MFGTIASLNVSVACGAFLYEVVRQRQ"
     gene            complement(28510..>29979)
                     /locus_tag="P5335_01760"
     CDS             complement(28510..>29979)
                     /locus_tag="P5335_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73635.1"
                     /translation="GFLESENLGNIKTNDSTFLFRYKLGKRISFLHIYIGKENSELIQ
                     ENYELKNGVLILPIASVESFMNLQLKKLEQKGYSLANLKLENFRTEDSLLFADLKIKK
                     NSQRILNDIVINGSSKFPESHKRNLMRLYANRTFNQDGLQKVFKEIEEYRFVKQTKYP
                     EILFTQDSTKIYVYLEKAKSNRFDGFIGFSNEEDGKFSFNGYLDILLHNILNSGEQFL
                     IYWKNDGKKQTSFNIGLQVPYLFKSPIGIKAELNIFKKDSTFQNTNTSIDLGYFFNYN
                     SQLYLGYQATESSDIQNVNSSFISDFTNSFITSQYEFTKFKTDDFLFPEKTVFHFKIG
                     LGKRDSKTDSQNQVFGKMELRHNFYLNAKNIIHLKSQNYLLTSENYIVNELYRFGGIN
                     SIRGFNENSLQGNLFTSLLTEYRYVLSPGMYVHSIIDYGYYQDQTTALNDSLIGLGFG
                     FGLLTKNGLFNLIYANGSTNDQAIKLANSIVHISFKSSF"
BASE COUNT         9795 a         4596 c         4993 g        10395 t
ORIGIN      
        1 actttctcgt caattcgagc cgggcaaatc acatattcta cttgattggc ttcgggatga
       61 aattccattt ctaccaccgg ataggttttt acttctccgg aaaccgatcg agtgacaatg
      121 accgccaatt catttttgaa aggaatcatt tcttcaatta aacatggtgt ttcgggcaaa
      181 tcaactaaat cattttctga ttttagaatt ttcacgccgt aaccatcata accaaaacgg
      241 tcttgcttcc atacgcatgg aaaaattaaa tttccttgtg aaacttctac taataaatct
      301 tttttatcat caaaaggttt ggcattggct gttggaattt gatattggag atataatttt
      361 ttttgagtga ttttgctttg aatatttcga agtgttttag gtgaaggaaa tactttttta
      421 ccttccgctt ccaatttatc taacgcatct aaattcacgt tttcgatttc gatagttaat
      481 acatctactt tcgtaccaaa gttgtaaacc gtatcaaaat ctaagaggtt cccaatttca
      541 aaataatgtg ccccgaattg acaaggagcg tctttactcg ggtctaaaac ataagtttga
      601 atgtcaaatt ttcgtgtttc agccaagagc attttgccta attgaccacc acctaaaata
      661 cctaatttaa aatcggatga aaaataattc atttgttgta gttattgttt tttaaagtca
      721 aatgcagtca catcatttgt gtcattgtgt tgttgtttgg acaaagataa gtaacaattt
      781 taggactcaa aacttatttc agccgtttta aaatattcct ggcagccgcc ttgtatgccg
      841 cagattgatt cgaataatcc tgacttaata ttggttttaa ttcctcgtaa atccaaggat
      901 atttttttcc taaaataaat aaagctttca ttgcatatac ttttgaagct acttttacat
      961 cctgtattaa tctgtcaatc atggcttcaa taagcaaatg ttcttgttct tttgttaaat
     1021 taattccgtt ttttcgatga ttgcttttta ctaaaaacat tgcaattcgg gtagctggtc
     1081 gtaaagaaga atcttctttg atatgcggta aaactccaca aaaatcatca aggtaaggaa
     1141 ctagtaattt taactttttt tcaccaatca aatctaaact ccaaaaggct ttcacatgta
     1201 aatcgtgatt tatatcaaaa gcaatcgaaa ttaattctga taaattttct tcacttttca
     1261 tagcaaagtt actcaccgta gttctacttt taacatgagc tgtgctttgc tcaatttttt
     1321 gatatagggt tgaattcatt ttgtaaatat atcaattttg caaaaaagtc taattactta
     1381 gcacgtaata cgtactactt ctttaaattt gcattttatt taaaaaacca caagatgata
     1441 caccttcacg acaaaacttt tgagcccttt atttcctcag aagaaattga ttttgcagtt
     1501 tccaacatgg cgaaacaaat ggacgacgat ttttttgatg acatacccgt atttattggt
     1561 gttttgaacg gagcttttat ggttttgtca gatttgatga aacaatatag gggcatgtgt
     1621 gaagttagtt ttttaaaatt agcttcttat gaaggaacac aatctactaa cgaagtaaag
     1681 caattaattg gtttaaatca aaatttggaa ggcagaacgg tagttatagt tgaagatatt
     1741 gttgacaccg gtaatacaat cgaagaattg aaagtgattt ttaaagagca taatgtgaag
     1801 catttaaaaa tagccacttt gtttctaaaa ccggatgctt ataaaaaaga aattaaaatt
     1861 gattatgtcg gaatacgaat tccaaacaaa tttattgttg gttatggttt agattatgac
     1921 ggattaggca gaaatcttcc ggatgtttac caactagccg aataatataa aaaatttata
     1981 atttataaat caaaatttaa aatgatcaac attgttcttt tcggaaaacc gggtgcagga
     2041 aaaggcacac aagctgaatt tttaaaagag aaatataatc ttactcattt atctacagga
     2101 gatattttta gatttaatat caaaaatgat actgaactag gaaaattagc caaaacattt
     2161 atggataaag gtgatttggt acccgatgaa gtaaccattc aaatgttgca aagtgaagtt
     2221 gataaaaatc caaattcagc cggattttta tttgatggtt tcccaagaac tatcgctcaa
     2281 gcagaagcct tagattcttt tttagtatct aaaaagcagg aaattacagc aacggttgcc
     2341 ttagaagctg atgataatat tttagtgcaa cggttattgg aaagaggaaa aacatctgga
     2401 aggccagacg atcaggatga agaaaaaatt agaaacagat accaagaata caacgaaaaa
     2461 accgctccct taatggagta ttataaaaat caaggtaaat ttcatgcggt aaatggaatt
     2521 ggatcgattt tagaggttaa cgaacgatta aactttgtct tcgacaactt gaaataaata
     2581 gttatttatc taaaatttgg caatttagtc ttgaattttt tagatatttg cagaattaaa
     2641 aaggagcttc atccgtaata tcggaaatga tgctcttttt ttgctaaaaa aaacaaatgg
     2701 aaattttaat aattttattt ttaattttgt tgaatggagt tttttctatg tcggaaattg
     2761 cactnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntaattt tatttttaat
     2881 tttgttgaat ggagtttttt ctatgtcgga aattgcacta atttcagcta gaaaaagtcg
     2941 tttagaaact gctgctaaaa aaggaaatcc tgctgcaaaa gtggccttag atttagccaa
     3001 ctcacccaat aaatttttgt caacagtcca aattgggatt acactaattg gaattttgac
     3061 aggtatttat tccggagata aaatcactac tgacgtaaaa aattttgtta gtggttttga
     3121 aatgttaaaa ccatttgcag actcaattgc tgttggtatt gttgtagttg ttttaacttt
     3181 tttttcactt gttttaggtg aattagttcc aaaaagaata ggtttgaatt atccggagac
     3241 cattgcaaaa gcggttgctg tacccatgaa aattgtttca aaaattaccg ctccgtttat
     3301 ttggttgtta accatttcta ctgaatttat tttagattta ttcagaatta aacctactgc
     3361 cgatggtaaa gtgacagaag aagaaataaa agccattatt aaagaaggta ctgaagtagg
     3421 ggaagttcag gaaattgagc aagatattgt tgaacgtgtt tttcacattg gtgatagaaa
     3481 agtaaattcg ttgatgaccc atcgatcatc tgtagtttat ctttccttgg aagatacact
     3541 tcaagagcta aaagcaaaag tattggagga gttacattct ttttatcctg tttgtggtga
     3601 aaatatggat gatattgagg gaattgtatt gttgaaagat ttgttttcaa gctttgaaaa
     3661 aggtagtttt gatttaaaaa caattaccaa agaaccggtt tattttatcg aacatacatc
     3721 tgcctacaag gcgttggaaa attttaaaac atcaaaagtt cactatgctt taatagtaga
     3781 tgagcacggt gtttttcaag gaataataac tttgaatgat attttggaag ctttagtggg
     3841 agatgcatcc gatttttatg aggaagaatt taaattagtt gctcgagaag atgggagttg
     3901 gttggtagac ggacattatt cattacacga ttttcttacc tattttgata tggatgaaaa
     3961 tgttaatgat tatgatgtaa ctaccgtgag cggatttatc attactgaat taggaaatat
     4021 cccgaaacaa ggacaaaaat taatttggaa taaattagaa tttgaagtta tcgatatgga
     4081 tggtgttaaa attgacaaaa ttctaattaa aaatttaaaa gaataaagaa ttaggggtca
     4141 gttaatagtt atttagtaat gcaaaaaagc aattacagat aattgaaaac cgacaacaga
     4201 aaaaaaaaac aatgacagaa ggaaattttg tagactacgt aaaaatatat gtttcatccg
     4261 gaaaaggagg gaagggttct tctcatttgc accgtgaaaa atttattgaa aaaggtggtc
     4321 ctgatggtgg agacggaggt cgtggaggtc atgtttattt aaaagggaac aaaagtttgt
     4381 ggacactatt tcatttgaaa tttgtaaagc atattcgtgc cggacatggt ggagatggtg
     4441 ggagttccag aagtaccgga catgatggag aagataaatt tattgaagtt cctttaggaa
     4501 cggttgtaaa aaatcaggaa accggtgaag ttctttttga aattacggaa catgatgagg
     4561 tgaaaattat tgcaaaaggg ggaaaaggcg gtcttgggaa ctggaatttc cgttcatcaa
     4621 ccaatcaaac acctcgctat gctcaacccg gaatgccggt ggaggaagcg gatgtgattt
     4681 tagaattaaa agtacttgct gatgttggtc ttgttggttt tccaaatgcg ggtaaatcta
     4741 ctttactttc tgttctgact tctgctaaac ctaagattgc tgattatgct tttacaacct
     4801 taaaaccaaa cctgggaatt gttgcttatc gcgagtttca gtcatttgta atggctgata
     4861 ttccgggtat aattgaagga gctgctgaag gaaaaggact tggacattac tttttacgac
     4921 acatcgaacg aaattcaatt ttattgtttt tagtccctgc cgatgctgcc gatatcaaga
     4981 aagaatatga aatattgtta gatgagcttc gtcgatacaa tcctgaaatg ctcgataaag
     5041 accgtatttt ggtggtttca aaatgtgata tgttagatga agagctaaaa gcggaacttg
     5101 aaaaacaaat gaaaaaagag tttaaaggag tgaagtatca ttttatttct tctgtggctc
     5161 aacaaggctt acaagagttg aaagatatgt tgtggaaaat gttgaatgca gaatagtaaa
     5221 aaaatacata tttagtacaa aagcatccct agaaggatgc tttttttatg gaattctaaa
     5281 aattaagtta aataatacga taacgttata gtttttcagc ttgaaattga aattgaaaac
     5341 acaaaaaaaa gtatattcgc agacactaaa ttttacacaa aagatgaaaa aaattatttt
     5401 aagtttcgtg gcattaattg catttgttcc ggcaacagtt tttgctaacg aagggatgtg
     5461 gtttttgatg catattcagc gattaaatca ccgagatatg caaaaaatgg gattgcaatt
     5521 aactgctgaa gaaatataca gcattaataa ttccagttta aaagatgcaa tcgttcaatt
     5581 caatggtggt tgtacagctg aaatgatttc taaagatggt ttggttttaa caaatcacca
     5641 ttgtggatat gatgctattg ccgaattatc gacaactgaa agtaattatt tgagagatgg
     5701 attttgggct caaaatcgta aagcggaatt gaaacctaag agtttatatg tgcgtttctt
     5761 tgttcgtatg gatgatgtta caaaaagaat gttggctgta gttaatgata aaatgactga
     5821 agctgaacgt gaagcagcta ttaatcagga aactgctaaa attcaaaaag agaatgatgg
     5881 tggcggaaaa tatactgttt ctgttcgtcc attttttcaa ggaaatgaat attattattt
     5941 cgtttaccaa gattataagg atgtacgatt agttggcaca cctacagaaa gcatcggtaa
     6001 atttggtgga gatactgata actgggaatg gcctcgtcac acaggagact tctctatgtt
     6061 tagagtgtat gctgatgcaa acggaaatcc tgctgaatat tcagaaaata atgttccttt
     6121 aaacccaaaa caccatttac caatcagttt aaaaggagtt aaagaaaatg attatgcgat
     6181 gattttaggt tatccaggaa gaacaaatcg ttggatgcca gcggaaggaa ttgagcaaaa
     6241 cgtaaaatat gcttatcctg cctgggttga ggggtctaag atgggtatgg acaaaatgaa
     6301 agtccacatg gataaagatg ctgccgttcg tttaatgtat gcttcaaaat atgccggtgt
     6361 tgccaattat tggaaaaacc gtcaaggtat gattgatgcg ttaagtaaat ttaaaacagc
     6421 ggatgcaaaa cgtaaaaatg aagctaaatt taatgcttgg gcaaacaaac cggcaaacaa
     6481 agctaaatat ggtgaagtag tgccaaccat caataaatat tatgccatga ccaatgaaaa
     6541 agctcgtcat gacaattatt tacaacaatt gttgagaact agtgcttttg gaacaatttc
     6601 cagagggtta ggaaaacaat tggaaggata tgctaaagct gatgtggcca aaagagccca
     6661 attaaaaccg ggtttacaag aaatgataga tggttttttt ggagaagtat atttaccggc
     6721 agaaaaagat attttagcag gtcaattagc tttgtataat gcaaaagcag gatataaaat
     6781 tgctccgtct gtagaagaaa tggctaaagc aaacaacaac gactttacgg cttatgttaa
     6841 tgcagctttt gaattaagta tgtttacatc aaaagaaaaa ttgaatgcct ttttggaaat
     6901 tccaagtgaa gctatgataa caaatgatcc gttaaataaa ttatcaaatg atatgctaac
     6961 gcatttaaca actcgctctg atgagttgac tcaagctcaa aatgattatt caaaatcatt
     7021 ccgtttgtta gttgaaggat taagagaatc aaaattaagt ccaattcaat atcctgatgc
     7081 caattcaacg ttgcgtttaa cttatggaaa agtaagagca ttgcctgcgg ataaaagaaa
     7141 tgatgctaaa attaataact atactacttt agaaggtatg gttaaaaaac acaaagcagg
     7201 tgatgaagag ttcaatttat ctcaaccgtt aattgatttg tataataaaa aagattttgg
     7261 tcaatatgct gataaagatg gttcattgca tgtgaacttt ttaacagata atgatatcac
     7321 tggaggaaat tcaggttcac ctgttctaaa tggaaaagga gaattaattg gattagcttt
     7381 tgacggaaac atcgaagcaa tggccggaga tgttattttt gatgacaaat tacaaagaac
     7441 aatcaatgta gatatccgtt tcgttttatt cattattgaa aaatatgccg gagcaaaaca
     7501 tattattgac gaattgacaa ttgtcaaata aggtttattg ttattattaa ttcattaaaa
     7561 aatctgcttc agcaatgaag cagatttttt ttatgataga attttgagat taatctattt
     7621 ttacttttgt actgatggaa tgtgcagaaa attctggagc atacatgctc tgtaaagttg
     7681 aaattccatc gataaaacta ccggaatgat taacacgaac atcatattct aaaacataag
     7741 tccctctctt gattgtgtca aagaaaaagt gtgtcgcaac atcttttgta cttctataaa
     7801 agcgtaatcc gtcttttaat tcatatttcg aaataacatc tacaggttca aaacaagaag
     7861 ctcttaaatc ttttaaatga acaaattcta aatcattttc agtctttata attaatcgga
     7921 tggtaattaa atcgccctgt tttagcgatt ctttatttag ttcaactaat tgattaccaa
     7981 atgatgattt tacttttttg tacacatttt ttgtaaccga gagaatagca gtactatctg
     8041 atttgatgtt ctccaagttt tcaaaatatt gccaatacaa tcccccaaat cctgcaactg
     8101 atgatttgtt ttctactgaa atcaaaccca tttcttttgt gatttcatcc gaattccaat
     8161 tcattttgat gtaaccggtt tcggcttctt tatctttttc aattaatttt ttggttaaca
     8221 ctttttcgtt gccaatttta aatttggtgt tgtctttgat gtttatccaa tcatttcctt
     8281 gcaacaataa agcataaaca gcttctgttg tggcttttgt agttggccaa ttttgtaatt
     8341 gcttttgttt gagcaaccaa actttcattt catctacata ctttttgtct ttttcaattt
     8401 ctgcaaaagc ttcaattagc aaagcttgcg tttctatgga agattgatgc caatagtagc
     8461 catttttgtt ttcaatccaa tacataccaa aatcatcatt tatagcaacc gtttctttca
     8521 agttagcgat gatatttttt gcaaaatctt tatcacctaa acgatacatt gttaaagcta
     8581 aaagtgcttt tttgtataag gaatactgca accaattcgc tttgaattct actttttgaa
     8641 tagtaataat cgaatctgtt tttttagaaa ccggtaattt ttcaatataa aaacttcgag
     8701 catacaaata atgtaaatta gaataagtat aataattaat acgttcgttt tttaaagtgc
     8761 tagttttgat gaaattatta tcaaggtgtg gaattgcttt ggatgtaatt ttttcaaatt
     8821 tggaaatact ctccggaaat agttttccca aatgacctaa gccggcaatg atgtgttgtg
     8881 tgataaacat attttcatca ccaccatcaa accacggaaa tgctccggaa gacaattgtt
     8941 tttcttctaa ttttttaaat gtattttctt gtgattcttt cattgtattc aaatccatga
     9001 gcaaagccaa tcgattgttt ttcaattctt cactttctgc atccaaaagc caaggtgttt
     9061 catttaaaac aatagatttt agttcttcat tttgatttag ttttgaaaca gatgttgaat
     9121 tagttttcca agattcaaat aaagtagcaa ttttcggatt actgttgatg atttcggtag
     9181 caatgaaatt gccataatat cgtgaaaaag tctgttcagc acattcatgt tgaaattcca
     9241 tcaaataagg caacgattgc aatgctagcc atgttggatt tgatgtatat tctaatgtaa
     9301 attgatgatt ttttagtgtg gaggacgagt tgttttttag gttttcaaaa acgtattcct
     9361 ttttagaatt tcctttcacc caaatcggaa tactttcagt aatgagaatt ttgttactca
     9421 tcacaggaag aatattctct tcaccatcag aataattacc tgattttgcc acaattttat
     9481 actgtaaacc ctgtaaacct tccggaattg taattttcca tgttaccgga acactttctt
     9541 tgggtttgca atcaaaattt cggatgttat ttgggtttaa tgctatgctg tcgattgctt
     9601 tcatatttgt tgcatcaaac agcatcagca ttgcaattcc ggactttgta tcattggtca
     9661 tattcaccac ttttgcagaa attgatatcg aatctttttc tcgaacaaat cgtggcatat
     9721 tggtttgaac catcaaatct ttttgcgaaa taatagtgga ttcaaaatag ccagattctg
     9781 tttttttgtt atgaccgtac aaacgcaatt tccaagttgt taatgattct ggagtattaa
     9841 agttgaaact gaattttcca tcactatcgg tttttaaatg aggataaaag aaagcagttt
     9901 cattgaaatt tgtacgagtt ttgacttgag taagttcttt aaaagcattt tttgtggtaa
     9961 tgataactac gccattagca cctcttaatc catacaaagc tgcagcagaa ggacctttta
    10021 aaacggatat ttctttgatg ttttccaaat ctaaagtaga tgaatctcct tcaaatggaa
    10081 ctccatcgat aacatataaa ggttgattgt ctccttttat tgaagaagca ccacgaagta
    10141 taatacgagt tgaacttcca ggaacaccag atgagtttgt aacttgtacc ccagaaattt
    10201 cgcctgttaa agcagtaatt aaatctgtcg cattcatttc agctaatgta ttgctgttca
    10261 ttgattgtga agcataagtt actgatttct cctttttctt gattccataa gctccagtaa
    10321 ccaccacttc ttctaaataa ttctcactat ctttcaaatt aatataataa tcttttttat
    10381 ttccaattat aatttctatc gagttataac caacaaaacc gataaagagt acttcaccgg
    10441 gagcggcttc aatgttgaat tgaccgtcaa aatcagttga cgttccacgg tttgtgtttt
    10501 ttataatgac agaagcaccc ggaagtggca ttcctgagtc atctaaaatt acacctgtaa
    10561 tattttttgc gttattaggt aattcggtta agggcttaat tttatcaagg tatttttttt
    10621 gagtttggct attttttgga taagtatagt taaacccaaa ccaattaatt tgaggatttt
    10681 gaatgtaata ggagtaatat tttcgatcca tcttaaaatt gttaaatgtt ttataactaa
    10741 tatttgcgaa ttttaaatct ggaaaatttg tataatcaga agaatctgaa aaattaacag
    10801 ttgaccaatt ttgtgtatca aattgatcta acgacctgtc gtacatgctg gccaaaactt
    10861 ctgattccaa tttttggtca attattttaa aggaccaatt ttccaaactt cccggttcaa
    10921 ttttgtttcg catgcttaca atttcaaaat ttaattttgg ttcaattgtt tctttttgaa
    10981 ttgaatgagt ttttacagct gtgttatttt cccaaacaga agaaaaataa aaatgaacat
    11041 ccgttttgta ttccgatttt ttattaaact tgagaataac ttttccattt tttaattgaa
    11101 ccacttgttc attttccaat gtgttgtttt caccataaaa ccgagatgtt atccacaaat
    11161 caggaatagg ggatagaatt tctatttcaa ataaatttcc gttaggtttt gaagtgtctt
    11221 tgaaagtaaa caattcatct tttgtgttgg gacttacttt tgagtctaag ttgaaatttt
    11281 tttcggtttt aatgagatta ttttttttat cataagcttc tgtaaccact ttgtaatacc
    11341 catttttaaa agactttaaa aaatcaaagg aaatctcatt gctggttttc gtgtcaaacg
    11401 aaattgtttt tactaatact tcttttgttt catagtcaga ttggtcaaaa ggttcgtggg
    11461 ggaaaagttg ctcaaattca tttctgctga gggtttgtat ttccggaatt ccaaaaagcc
    11521 gattttttaa aaagatgctt ttttgtaatt cataaatttt tatttcacct tttgaatcaa
    11581 tagtataatt gtttaaagta gtgcttttta taatgatttt attatcgtct tctttaaata
    11641 agttactgtt taatagcaca tttaattcaa gcatttcttt cgcaactttt atatatttaa
    11701 aggcacttct agtttcgcct tgaatgtcag tgacatcaat ttttacagta tacctaatca
    11761 ataagatact gtcattagaa actccctctt caatagcagg aaattgaatg ttgaaattac
    11821 cattttcatc agtagccgtt tcagcaatta taaaatcatc atcgtcagaa aaagaacttt
    11881 tattcgaggt tgtttttttt gaaactgtat aagctacttt agcattagtt aaattgtttc
    11941 ccgctaatgc ttttgctttc cccttaattt ttatagtatc actaatcgta taattttctt
    12001 taatttcatc aaaaattatt tcaaatgtag ggcgtttata ttcttcaact ttaaaataga
    12061 aattgtcaac atcaaaatct acgttatccc aaaatttgtg ttcttcttca ttactatcat
    12121 aatatttagc gtccatttca tagttatcgg cttcatcaat tgttatataa aaatttccgg
    12181 ttagcgtgtt tttcggaata tcaaattcac cagaaaatga gccaaattca ttggtttgaa
    12241 catcaaattc ttttaaaata ttttcattca catcattaat ttcaacatga atactcacat
    12301 aaggaaccac actttttatg ttgtctttat tttgaattaa tattcctttg tagtgtattt
    12361 tttgtccagg acgataaatg gcacggtcaa aataaaccat ggctttcgct tcaaaattat
    12421 caaaatcatc ttcattgttc tcttctcttt catagataaa agttctgtgg tactttttga
    12481 gtaaagtatc attttcttta attattaaaa tatcgttatt atatatttta ttctgaattt
    12541 gttttctcaa tctaaattgt gcttttcctt ctttgtttgt ataatcagtt tcatcctcat
    12601 ttttaatgct cacattttca ataggttttc cggttttttt gtctaataca taaattaaat
    12661 catacttttc atttttatct tcaacaataa aaaagttaga tacttggaaa ttttcataag
    12721 taaaagcaat tttcttttct atggtgtcat tagtcgtttc caaaaaaacc aaataattac
    12781 ctacatctat tttttctaaa ataatttcag ttgaatgatt gaaaaaatca ttagatgatg
    12841 ttaattttcg tgtaaaactc ttgattggtt tgttttgttg aacataattt ataattaatg
    12901 aatctttatt ctttttataa ttagtatcat aattatatga atttgtcaat aaataattat
    12961 ttttcactgg aaagaggtag taactaatct taatagaatc tacatttttg taatctaaaa
    13021 aggcacgatt gttttgattt ggatatgtgg ttgtctgtaa tctaacagat aatgattttt
    13081 gcagaatttg attttggaga gtctcagctt ccgccaaggt atttggatta atttttgtat
    13141 ttaaaacagt atcaattaat gcaagtgctt ctttgtattt gtttatttta ttattttttg
    13201 aagtttgact gatataattt ttaatgcgtt caattcttaa cgattgtttt aaaaatgagt
    13261 ttgaagtgat gttctccaat tgcttaattt tttctggata aacggaatat tcactgtaaa
    13321 gggaatgaaa atattttaat cgctcaaaat aagcaaaatc tgctttttct tttttttcag
    13381 ataaataata gcgttcatta ttttgtaaaa tttttagaag tttaatgaga ttgtcatcta
    13441 ctaataaagt taaattatat agcacgaata ttgtagattc ggcatataaa ttttgaaaca
    13501 agtctgattt gtttttttgg tcgaaatttt ttattttgga tttatagtga gccatatttt
    13561 tttccactaa aaaatcatat aaactataat tttttgcatc aatgctagga gaaacttcaa
    13621 atatttcttt gtaatcattt attgtagtag ctcttagaga tttttcattt tgcaaggaat
    13681 tctccatggc cttttcaatt tcatcaatat aatcagaagt tgaccaagtt gaaaaatctg
    13741 tgcttttttg attttctaaa tcatttcttt ttcctatctt atatgaattt tgattatagt
    13801 aattttgcag cagcgtagcg tagatataat ttaacaatgc ttttgaagca ggttttgctt
    13861 cctttatttc ttctttcaga gttgaaataa ttgtagattg agccttttcg ttcaaaactt
    13921 gttcaaattt tgatacgtaa aaaaaacatt ttatgatttg gacttcctct ttttttcgct
    13981 tggctttttt ataaatttct tgcacttctt tttgagctga ttgaattttg ccgtctaatt
    14041 caagtttata tacttctttc cattgttcat caaaattttg tccgaatgag taggaagcaa
    14101 ttaaaatgca acaaatagtt atagttttat acatgagtgt ttttttatta ggcgaaaaaa
    14161 gacaaagttg gtctggttgt attaagtcaa gttaattatt cactaaaata cttaaatatt
    14221 ttataagtaa aatgaatttg aaaaacattt ccttattttt gaattcaaat tatacccatg
    14281 caaattttca aagtagtaat tcttttattt tctttacaat tgatggcaaa ttccgacttt
    14341 gataaagccg aaaaattatt tcagcaaaat aagttggctg aggctcaatt actctttgaa
    14401 aagtatttaa aagaaaaccc tactcatacc aaaacactag aatatttagg tgacattgcc
    14461 ggagctaata aaaattggga taaagcgatt gtgtattacc aaaaaataaa aaatcaatat
    14521 cctcaaaacg ctgtttatca atataagtat ggtggtgttt taggaatgaa ggcaaaagaa
    14581 gtaaataaat ttaaagcatt gggaatgctt ggggatgtag aaaatgcttt tttaaatgct
    14641 gcaaaattag atcctaaatt tattgatgct cgttgggctt tagtaatctt atatattgaa
    14701 ttgccgggaa ttgtaggtgg tagtgaaacg aaagctcaaa aatatgctga cgaattgatg
    14761 aaactatcca aagtagatgg ttttatggct aaaggctata ttgatgaata tttcaatagg
    14821 tattcaaaag ccgaaagcaa ttataaaaaa gcacacgaaa ttggcaattc caaaaccacc
    14881 tttcaaaagt tatatgattt gtatcaaaag aaaatgaaac aaccacaaaa ggcagcggaa
    14941 ttaaaaaaac aatttgaaaa ataatagacg ttttctgttg taagttatct gttaactgac
    15001 aaccgaaaac ttataactga ttacaaaaaa tgagaaccca tttcatagct attggcggag
    15061 ccgccatgca taatttagca ctagcattac accacaaagg ttaccttgta accggaagcg
    15121 atgacgcaat ttttgaaccc tctaaatcac gattacaaaa acacggactt ttacctgaag
    15181 aaatgggttg gtttccggag aaaataacat caaatattga agcaattatt ctcggaatgc
    15241 atgccaaagc tgataatccg gaattattga gagctaaaga attgggacta aaaatatatt
    15301 cctatcccga atttttatac gaacaatcaa agaataaaac ccgtgttgtt attggtggtt
    15361 cacatgggaa aacaactatt acttcgatga ttttacatgt gatgcattat cacaatatcg
    15421 aagtcgatta tatggtagga gcacagttgg aaggttttga tacgatggtt catctcaccg
    15481 aaaaaaatga tttcatcgtt ttagaaggcg atgaatattt gtcatctcca attgatagaa
    15541 gaccgaaatt tcatttgtac caacctaata ttgctttact ttctggtatt gcttgggatc
    15601 atattaatgt gtttccaacc tttgaaaatt atgtagaaca gtttgaaatt tttgtcaatc
    15661 aaatcaccaa aggtggaatt ttggtatata atgaagagga tgaaacggtt aaaaaagtgg
    15721 ccgaagaaac taccaatact attagaaaat tgccttatca aacgccaact tattccgttg
    15781 aaaacggcac aacttattta gacacttcgg aaggaccaat gccaattgag gtttttggag
    15841 ctcataacct aaacaatctt gccggtgcca aatggatttg tcaaaacatg ggagttgatg
    15901 aagccgattt ttacgaagcc atagccagtt ttaaaggagc ttccaaacga ttggaaaaaa
    15961 ttgcagaagg aaaaggaaaa gtggcttata aagatttcgc tcattcaccc agtaaagttt
    16021 ctgctactac caaagccgtt aaaaatcaat acccggatag aaaattagtg gcttgtttag
    16081 aattgcatac ttatagcagt ttgaatgccg aatttcttaa agaatatgaa ggagctttag
    16141 atgctgccga tatagccgtc gttttttatt cgcctgatgc agtaaaaatc aaacagttag
    16201 aggaagtaac ctacgaccaa attgcacaat ctttcaaaag aaatgatttg attatttata
    16261 cgaatccaac cgaatttaaa gattttttat tttcaaccaa tttggataat tctgctttgt
    16321 tattgatgag ttcaggtaat tatggtgggt tggattttgt ggaggttaaa aatctaatta
    16381 actaaatttc attatatttc taatatataa aaatgctcct ttgtataaag gagcattttt
    16441 tctaaattaa tatttaaaat tagtctttta agaattcttc aaacgttttt aatgtaaaag
    16501 gaatttcaag ttttgccgct tgttccatca attttgattt gtatttccct tttaaatctt
    16561 taaaaccgga tgctgccgta ttaaatttat ccatcacatt ttcaatattc gaaacttgtg
    16621 agggagatgg agcatcaaat tgtgaagcaa ccgtggcata aatagtactt aatttttctc
    16681 ttaattgagg ctcagcagct cctacataca tatcacccgt ggtaaccacc attgtgtttt
    16741 ttaatgaatt taattccgaa tcaattttgc catttgtttt ggctgatttt ggatctttct
    16801 ccattatttt tttgtttaac gaaatcattt catctaattc ataaaccata taagctaatt
    16861 cttcattttt atcaaaaagc attttagttg tttcagcctg tttgtttcgt tcagcttctg
    16921 aaatgacact ttttggatca tttaccactt ggaattcttt tacataagtt tcttttcctt
    16981 tggttaaaac agctttatat gttccggcgg gtactcttgg tgctgtaaat ccaccataac
    17041 ttagtgtttt agcggttgct aatttcggat ttttggtatt gaatccccaa gtaacaatgt
    17101 tgattccttt ttgatttccc ggagcaagag ttattatttt attacctttc atatcttgaa
    17161 tttctaaatc catttttccg aaagtattac gttttttcaa ataatagact aattgagccg
    17221 atgtatttgg attgggaccc acaaattcta attcagaagc tgtcccgcca aaactcgatt
    17281 gttcttcaat aacaaaagat ttagtgtcaa aaaaatgcac ttcttttttc aaaatatgtt
    17341 gatttacttg tcgtaacgga cttatatcat ccaaaataat gattcctctt ccatgtgttg
    17401 ccatgactaa atcattcgtt tttggatgta aatcgatata atgaacagct acagctggca
    17461 tttcattttc aaattttgac caattttttc caccatctat ggtgatgtat aacccttttt
    17521 cagttcctaa aaataataag ttaggatttt taaaatcttc ttggatattt ctggcaaaac
    17581 catctaaatc ggcagtaaca attgaattcc aagttttacc aaaatcggaa gtcttataaa
    17641 cgtaagtggc ataatcattt ttggaatgtc catcaaaaac cgcataggca gtactcgcat
    17701 caaaaacact ggcttcaata tgataacacc aagtattttt tggtaaccct tttaaattca
    17761 aagttatatt tgaccaagtt tttccaccat cttttgtgat ttgaacattt ccgtcatcgg
    17821 taccagccca aagaacttct ttgtttaaag gtgattcagt aatagtgaaa attgtacaat
    17881 gattttcagc acctgaatta tctactgata aacctcccga tttttcagtg ttagttttag
    17941 aaggatcatt cgtggttaaa tcacccgata tttttcgcca agtttgtccc atatcgtctg
    18001 aaacatgcac aaattgactt cctgcatata ttctgtccgg attatgaatt ccggttacaa
    18061 tgggagtatt ccagttaaaa cgcaatttag ggtcgccttt tgtagctaaa ggttgaatag
    18121 tttttaattc tttagtatct aaattataac gccaaatgtt ttcagcaccc tgcatttcac
    18181 tgtaaataat ttttttggtt ggatgtttta aaacacgaaa accatcgcca taacctacac
    18241 tgtgccaatc tcgtgcttca ataccaccat cgcttttact tgggccatac caagagccat
    18301 tgtcttgtaa tccaccataa atattataag gttcttcatt gtctaaactt aaatggtagt
    18361 attgagaaag aggtaaattt ttaacgatat ccatcgtatt tcctgcatcc caacttctgt
    18421 ataatccacc atcggttgct acataaattc tgttactgtc aactaaatca attgtaatgt
    18481 catgaatgtc agaatgcatg ttacccaaac ctttaaatgt ttttcctccg tctctactga
    18541 tagaaccacg caaaccgcct ttgtaaataa tatccggatt tcttggatca accgtaattc
    18601 tggaaaaata gaacggacga acaaccaatt caaaatcacc attcaaatgt ttccaagttg
    18661 cacctgaatc atcagaacgg tataatcctt tgtctttatc ttgttctgtt tctaaaaccg
    18721 catataaaat ttcagggttt gatggagccg ttgctatggc aattctccct aattgacctt
    18781 taggaaaacc ggtatgaatt ttattccacg tttttccacc atcgattgtc ttgtataagg
    18841 cagaattatt tccaccggaa ttgaatgacc aagctgttct tctaaattcc caaaaactgg
    18901 catacataat atccggattt ttagaatcta tgaccaagtc agcacaacct gttttttgat
    18961 caacataaaa aactttgttc caggtttttc cgccatcggt tgttttataa attcctctat
    19021 cttcactgtc accccaaagt gctcctaaaa cccctgccca aactacatca ctatttctag
    19081 ggtcgacaac aatggatgta atacgatcag attttggtaa acccatatta ttccaattca
    19141 agccaccgtc agttgattta taaattccgt cgccaataga cacactattt ctagtccaag
    19201 gttcgccggt tcccacccaa attgtattgt ctgggttgtt tgggtcaata gtcacacaac
    19261 ctatagattg aatatgtttg tcgaaaatgg gagaaaaaga agctcctcca tcagaacttt
    19321 tccatacacc gcctccggca gcaccaatat aaatgatttt attattagtg gggtgattag
    19381 caatatctga aattcgaccg ctcataatgg ctgggccaat ttgacgggct ttaatatctc
    19441 cataaagctc ttttcctttt aatattactt cctcttgagc ttgagaaaaa cacaccgtaa
    19501 ataatccaaa taggtaaata attgtattta gttttttcat aaaattagct taattaaaaa
    19561 atccccaagt tttgagttgg ggattggtat tatttaaatt taaattattc tttaaaagca
    19621 aaagcacttt gatcaacagc aggattcaaa gtgatttttt taattttcat ggcttgccct
    19681 ccttgattca ttgaaaaagg gaaaaatata ccgtccactt cttgataatc actcatcgtg
    19741 gtagtggaga tttgaccttt agcagggcct gattttactt cagtttcagt tagaataggc
    19801 acattatttt cagtatcaaa ataataaaaa acaacgttgt ctactttttg cccatcaatg
    19861 gtttgaggtt tttttgtcaa ttttatttta tgacactcag tcccttcttt agtttctttt
    19921 cctaaatatt cagcagtata tcccttggct ttataatcaa gcatggcatc cggaaaatcg
    19981 tttgcttcgt ctttgatatt ttgaaccatt tcggattcta atttttccgc tttcatcgtc
    20041 ataaagtttg ttgaccatgc tgtggtaccg tcaaaagcca tttgtgtaat ttcttgccct
    20101 tgaaaattga ttttcaaata cattttacct cctttaagtt gaactagttc taccggaatt
    20161 tccatccctt gatagtttac ggtcatttct gttttggtac cggttaaact tttgaatttt
    20221 tcaacaccac caatattttt taaataagta cttataattt catctgctgt ttgagcacga
    20281 gttgaaaaac tgacaaaaac aagtaacatc atgcttacta aagatccaat ttttttcatt
    20341 gtagaagttt taaaatttat taataagtaa taaaaatagt aattatgtta cataaaattt
    20401 tattttttgt gtaattttat tttttttaaa ttatttaaac gccaaacaga atggaagata
    20461 ctacctcttt ttcaattaaa tattgggctg aagatgataa gcccagggag aagttaatgc
    20521 tcaaaggaaa atcaactttg agtgatgctg aacttttagc catacttata ggctcaggaa
    20581 gcagaaatga aagtgccgtt gaattaagca aaaagatttt agccagtgtt caaaacaacc
    20641 taaatagttt aggtaaaatg tcaattcaac aattaacaaa atttaaaggt attggtaagg
    20701 caaaagccat ttcaattata gctgccggag aattaggaaa aagacgtaaa gaggaaggag
    20761 tgcaagaatt aataaaaatc acttccagtc gaattgttta tgagattatg caacctatta
    20821 ttggagaatt gccccacgaa gaattttggg ttttatattt aaataatgcc aataaaatta
    20881 tttataaagc tcaacttagt aaaggcggga ttactggaac cgtagtggat gttcgattgc
    20941 ttttgaaaac agctttagag cacaatgcct tgtcttttgt tttaacacat aaccatcctt
    21001 cgggaaaatt aattgcgagt gaagcggata ttcaaattac acaaaaatta aaattggcgg
    21061 caaaacaatt ggatatgtct gttatagacc atattattgt aactgaaaac ggctatttca
    21121 gttttgccga cgaaggaata ctataattaa catactctga atgtctgaga agagaaaaag
    21181 tcaagccaaa ttaatccgaa tatttagaaa agtgcaccga acaacaggag cgttattgtt
    21241 tgtttttttc tttttaattt ctgtaaccgg atttttatta ggaataaaaa agcatagtgg
    21301 aggagttatt cttcccgaaa caaaaatcgg ctctacaaca caactaacta aatttttacc
    21361 tttagatagt ctgcaaaaga atgccagtcg ttttttggca gattatctta accaaacgaa
    21421 aaagatagaa attgacagaa ttgatcttcg acccgaaaaa ggaatagtaa aatttacttt
    21481 tgtatcaaac ttttatgaag ttcaactcga tggttcaacc ggaagtttat tgcaaattga
    21541 tttgcgacga tctgatgtta tcgaaaaatt acacgatggc tcaattgttg attattattt
    21601 aggattcgaa tctggagttt ttaaactcat ttatactatc attatgagtt tggctttgat
    21661 ggtttttacc attaccggat tctggctttg gtatggacca aaacaaatga gaaaatccta
    21721 agctaaattt tagaattgat taacaaataa tttttcattt tagcagtatc tttccacact
    21781 acaaataaag cttgtatgat aacaatcaaa aatgtgtttt tttcgtataa ttccacgact
    21841 gcatttcatt ttcctgatat gaattgttca gcgtcagaag cactacttat taccggtaat
    21901 tccggaaagg gtaaaacaac attacttcat ttactaggag gtttattacg tccgaaaagt
    21961 ggagagattt caattgaaaa taacaatatt accttatttt ctgaacaaga attagatcaa
    22021 ttccgaggaa aaaacattgg gttggtttta caacaatcac attttattgc gtccttaaat
    22081 gtcttagaaa atgtagtttt ggcctcatgg ttagccacag gaaaaagagc cacagaaaaa
    22141 gctaaagcac ttttaaaaga gttggattta gaggaccaaa tgtataaatt aaccgcagag
    22201 ttgagcgttg gccaacaaca acgtgtgtcc attgccagag cattgattaa tgaaccaaaa
    22261 ttacttttag ccgatgaacc aacttcaagt ttagatgatg aaaatgctgc aaaagtagcc
    22321 gatttgttag cccaattatc taaagaatac aaagcttctt taataatcgt aacgcatgat
    22381 catcggttaa aagaaaaatt ttctaaccat ataaatataa attgacatga ttggaaaatt
    22441 agcatggaaa aacctttggt ttaagccttt aaatacggtt ttaagtttag ttttattaac
    22501 tgcatccgta gcaataattt ctcttttaat tttattgcag gaacaatttg aaaaacaatt
    22561 ttcaagcaat attgatggag ttgatttggt tttaggtgca caaggaagtc ctcttcaatt
    22621 gatactttct tcagtatatc aagtagatgc tccaacaggg aatatcaatt ataatgaagc
    22681 agaagtttgg atgaaacacc catttgttga aacggccatt ccgctagctt ttggtgataa
    22741 ttatcgaggt tttaaaattg taggaacaac acccgattat attaaaaaat atacagccac
    22801 tatttccgaa ggaaaagtgt ttgaaaagaa ttttgaagtc gttatcggat gtgaaattgc
    22861 taaaaaactt aaaataaaag tgggtgataa attttttggt acgcacggag atgcacaaga
    22921 aggagatgtt catgacgaat ttgcctatgt agttaccggc attgcttcac caaccggaaa
    22981 ggtgatagat aatttaattt tatgtaccgt tgaaagtgtt tgggaaatgc attctggtca
    23041 cgatcatggt ggtgaagcac attctgaaga tgaaaatcca aaacatggag aggaagggca
    23101 tattcatgtg gatggtgatg agcaagatca tgaagaacat gttcacgatg aaaattgcaa
    23161 tcatgaccaa gaaatttctg aaaatccggc tcacggtgaa gaaggtcatg tgcatgaaga
    23221 aggcgatgat cacgaacatg cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23341 nnnagagttt ccggagcttc gcggttacaa ctcctagcac ttgtgctatt tgaaagtctt
    23401 ctactatgca tagttggatt tgtttttggt acacttattg gaagaatagc cttaatgatg
    23461 atatctgcta ccacagacga acaattcaaa atggcattta acccgatgat aattctttgg
    23521 gaaaaagaag gacttttgtt tttggttact atctttgtgg gtgtaattgc agcggtcatt
    23581 ccggctataa aagcttaccg actaaacatt tcaaaaacat tagcaaatgc gtaaaatata
    23641 tatctttttc ataattatct tcggatttat tattttttta gacagttcat ttatacctaa
    23701 aattggcgta aaccaaattg atacaaaaac tgtaaagcca tctttacaaa catccagttt
    23761 tatgcctgaa ttaatcgaaa atgatgctgt ttttatgcaa gtacctgata ccttatcatg
    23821 gaaattattg ggagtaatta aatttataaa aaaaccggat aaagactatc cggatggtgt
    23881 aatgtttcca attgtgaact caactttaaa agaaaaaaac aagaagaaaa ttgtcatgag
    23941 tggatttatt attcctattg acaacgtatc atatgcttta agtaaaaatg tttttgcctc
    24001 ttgttttttc tgtggtcaag ctggtcccga aaccattatg ggaatcaagt ttaaaggagc
    24061 gacaccaaag ctaaaaacag atcaatatgt aaccatggaa ggaactttcc gttacaatga
    24121 taatgatgtg aacgattgga tttatcatat tgaaaatgcc gtaattataa aagggggtaa
    24181 ataaatttat tttttgtgtt taaaaacaag acggatattt tttttgattt ggatcatacc
    24241 ttgtgggatt ttgaaaaaaa ttcagcacta gcttttgaaa aaatatttga aaaacaccaa
    24301 ttagatttaa atttagagga atttctgttg cattatattc caacaaattt gaaatattgg
    24361 aaattgtatc gcgatgaaaa aatatctcaa gaggatcttc gatatcatcg gttgaaagaa
    24421 gtatttgatt tgatgaataa ggaagttgtg gatgaaatga ttcatttatt gtctcatgaa
    24481 tatattcact atttgcctca atttaattac ttatatgaag gtgcttggga aattttagac
    24541 tatttatttc caaaatataa gttgcatatt ataacaaatg gatttcaaac tgttcaagct
    24601 ggaaaattga aaaactcaaa cattgaacat ttttttcaaa ccataacaaa ttccgaaatg
    24661 gctggagtaa aaaaacctaa tccgctgatt tttgaatatg ctttaaaaaa agcaaatgcc
    24721 acaaaatcca aaagtataat gattggtgat agtcttgaag cagatattga gggagctata
    24781 aatgttggct tagatgctat atttttcaat gaatttaatg tatcggttga tgaaaaaatt
    24841 catcaggtaa atcatttaat gcttttaaaa gaatatcttt aatttttaaa attcaattat
    24901 gaaaaaaata atttttgctt ttttgatcct tctttctaat tcaacatttt cgcaaacatt
    24961 gaatgattat caatatgtta ttgttcctgt tcgatttgat tttatgaaaa gcgacaatga
    25021 atatcgtttg aatacattca caaaatataa tttggaaaaa atgggtttta aggcatttta
    25081 taataatact gaaattccaa aagagattgt tgcgaatgct tgtgaagtat tgaaggtaga
    25141 ggtggaaaaa agcggtagtt ttatttggac aaatcttact attgttttta aagactgtca
    25201 aaacaatata gttcataaaa gtgcagtagg gaaaagcaaa gaaaaagact ttaataaagc
    25261 atatacagaa gctttaaact tagcatttgc ctcgttatat gagttgaact acagttataa
    25321 tggagcaact aaaagtttaa atataccggc gaattcaacg gttattgctt cagaagttat
    25381 agaaacttca gaagtgatta ccaagccaca aacaacaacc gaaaacagtt tgcaggcttt
    25441 gaaaataaat ggaggttatc acttagtcga tactgctcaa aaagttgttt tcaaattaac
    25501 ttcaacaggt aaagccgatt tttttattgc ccaaaaaggc aatcattccg gtgttgtttt
    25561 tcaaaaagaa gggcaatggt tttttgaata caatcaagag gataaattaa tttcagaaaa
    25621 attagaaata aatttctagt agccatattt atctttccat ctgtttttta aaaaagtacg
    25681 caattctttt tcacggggat tatttccggg ttcatatagt acattatttt gaagttcatc
    25741 cgggaggaat tcctgttctg cgaaattgtt ttcaaaatca tgagcatatt tgtattcgtt
    25801 tccatatccc aattctttca tcaatttggt aggggcattt ctcaaatgta aaggaaccgg
    25861 taagtctccg gtttgtttga ccaattgttg tgcttttcct atagccaaat aactggcatt
    25921 acttttagct gatgtggcta aataaatcgc acactgactc aataatattc tgctttcggg
    25981 ataaccaatg gttgtaacag cttgaaaagt attgttagcc ataattaaag cggtaggatt
    26041 tgcattgcca atatcttcac tggctaaaat caacattcta cgggcaataa attttacatc
    26101 ttcaccacct tcaatcattc tagccaacca ataaacagcc ccattggggt cacttcctcg
    26161 gatagatttt ataaaggcag aaacgatatc ataatgttgt tcacctgttt tgtcatataa
    26221 aacagtattt ttttggacca attgcattac tttttcattc gtgatttcaa tcggttcaat
    26281 atcggttgca ttgactatta attcaaaaat attgagtaat tttctaccat ctccaccgga
    26341 aagtcttagt aaagcttcag tttctatcag ttggatgttt ttacttttta aaacaacatc
    26401 attttcaatt gctctttgta acagttgctc aagatctgct tttgaaaatg aatttagaat
    26461 ataaacctga catcttgaca ataaggctgg aatgacttca aaactcggat tttcggtagt
    26521 ggctccaata agtgtaaccc aacctttttc gacagcagct aacaacgaat cttgttgcga
    26581 tttgctaaaa cgatgaatct cgtcaatgaa caaaattggg tttttagcag tgaataaacc
    26641 accactttgt ttggcttttt caatgacttc gcgaatgtct ttaactcccg aatttattgc
    26701 acttagcgta taaaatggcc gcttactttc ttgagccata atttgtgcta aagttgtttt
    26761 tccggttccg ggaggccccc ataaaattaa actcggaata attcctttag caatttggtg
    26821 cgtcaacgaa ccatgttcac ccactaaatg taactgactg atatattccg acaaatgttg
    26881 aggcctgatg cgttcggcta aaggtgcttc catggtgtaa aaatacagaa ttctgacaaa
    26941 aaagcactac tttcgttttg gttgtatttt tgattactac tttttgatga aacaaaaacc
    27001 ttttttattt tctacttctg taattgcctg gccacttttt tttgtgttgg ctatgtggat
    27061 ggtcttttgg gtagaggtcc gtttcaaagt agattttggt gattatggaa ttttacccag
    27121 atcttggagt ggattaagag gaattttgtt gagtcctttt ttacatggag atattaaaca
    27181 tttgtataat aattctattc cattgctttt tctgatagcc ttactgcgtt atttttatag
    27241 agaagtttct ttacaagttt tgatttttgg tattcttgtt tcagggcttg gaacttggat
    27301 tattggaaga gaaaattatc atattggagc tagtggactc atttatgtgt tggtgagttt
    27361 tatttttttt aaaggagttc aaacaaaata ttatcgatta gtggctttgt ctttagtgat
    27421 tgtcatgtta tacggaggaa caatttggta tatttttcct gaagttgata accaaatttc
    27481 ttgggaaggg catttggctg gttttattac agggtttttg ttttctatac tatataaagc
    27541 agaaaaatat aaaaagccaa ttcgatatga atgggaaaag ccaaatttta atcccgaact
    27601 agatccgttt atgaaacatt tcgatgaaaa tggaaatttt gtcaatacac caaaagaaga
    27661 agaattggaa tatatagaga ctcaaaatca atttacacct tcctcaaata ttgtctatga
    27721 ctatattcca aaaaaagaag aataaattac tgcctttgtc tgactacttc atataaaaaa
    27781 gcaccacaag ccacggaaac atttaaagag gcaatggttc caaacatggg tagtttagct
    27841 ttttcatcta caatttttaa aacggaagga ttgacgccac ggtcctcgct acccataata
    27901 atagctaaag gttcgttaag cgataagtca tataaagttg aatctgtttt ttcggtagct
    27961 gcgatggttt ttactcctga accttgcaga aaaaaaatag cgtctttaat atgttctact
    28021 ttacaaatag gtacattaaa tacagcaccg gcactggttt tgaccgtatc gccatttaca
    28081 ggagcggctc cttgtttacc aatgataatt ccgtccacac cggtacattc tgcggtgcgg
    28141 atgatggctc caaaatttcg ggcatcggat agttggtcta gaattaaaaa caaaggtgtt
    28201 ttaccacttt ctaaaacggt tgtcactaaa gtttctaaat catgaaaaga cacaggtgct
    28261 atagtagcca cagctccttg gtgatttttt gaagtcaatc gattcagttt ttcaaccggg
    28321 acatatgtga aatttacttt atgttgtttc attgttttca acaaatcccg catcaagtca
    28381 ccttgagcat ctttttgtaa aaaaacttta tctaattctt ttccggcttg aatggcttca
    28441 atgattgctc ttataccaaa tatttgattt tctttctcca tgccgcaaag gtacaattaa
    28501 gttaggaact taaaaactcg atttaaaact aatatgaacg atagaattgg ctaatttaat
    28561 tgcctgatca ttggtacttc cattggcata aattaaattg aaaagcccat ttttggttaa
    28621 taatccaaat ccaaaaccca agcctataag cgaatcattc aaggcagttg tttggtcttg
    28681 gtagtatcca taatcaataa tggaatgaac atacattccg ggagaaagca cataacgata
    28741 ttcggtcagc aacgaagtga ataaatttcc ttgtaaactg ttttcattga aacctcggat
    28801 agaattgatt cctccaaaac ggtacaattc atttacaata taattttcac tagttaaaag
    28861 atagttttga cttttgaggt ggatgatatt tttggcattt aaataaaaat tatgccttaa
    28921 ttccatcttt ccaaaaacct ggttttgaga atctgtttta gaatctcgtt tgcctaatcc
    28981 aattttaaaa tggaacaccg tcttttccgg aaataaaaaa tcatcagttt tgaatttagt
    29041 aaattcatac tgagaggtga taaacgaatt tgtgaaatca ctgataaaac tagaatttac
    29101 attttgaata tcgctggatt ctgttgcttg ataacctagg taaagttggc tgttatagtt
    29161 aaaaaagtag cctaaatcaa ttgaagtatt cgtgttttga aaagtgctgt cttttttaaa
    29221 tatgtttagc tctgctttta ttcctatcgg acttttaaat aggtatggta cttgaaggcc
    29281 aatgttaaac gaagtttgtt ttttgccatc atttttccaa taaattaaaa attgttcgcc
    29341 cgagtttaaa atgttgtgca ataaaatatc taaataacca ttgaagctga atttgccatc
    29401 ttcttcattt gaaaatccga taaaaccatc aaatcggtta gattttgcct tttcaagata
    29461 gacatatatt ttagtggaat cctgtgtaaa taatatttca gggtatttgg tctgtttgac
    29521 aaaccgatat tcttctattt ctttaaaaac tttttgaaga ccgtcttggt taaaagttct
    29581 attggcatat aatcgcatta aattgcgttt gtgactttcc ggaaatttag aagacccatt
    29641 gataacaata tcgttcagga ttcgctgact gtttttcttg atttttaaat cagcaaataa
    29701 aagtgaatct tccgttctga aattttccaa tttcaaatta gctaatgaat accctttttg
    29761 ctccaacttt tttaattgca aattcataaa actttccaca gaagcaattg gcaaaatcaa
    29821 cacaccgttt tttaattcat aattctcctg aattaattca ctgttttctt tacctatata
    29881 tatatgtaga aatgaaattc tcttgcctag tttatatcgg aataaaaaag tagaatcatt
    29941 tgtttttata tttcccagat tctcactttc taaaaaacc
//
LOCUS       DAOTCW010000011        27763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_863_length_27763_cov_48.239317, whole genome shotgun sequence.
ACCESSION   DAOTCW010000011 DAOTCW010000000
VERSION     DAOTCW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 27763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27763
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_863_length_27763_cov_48.239317"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="P5335_01765"
     CDS             complement(<1..212)
                     /locus_tag="P5335_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73636.1"
                     /translation="MKTLFAFLGLFMFASVSSTDLTESTITSNEPQLMETGEYSTSPS
                     KKRDLVMTTETGEYSTSPSKKRDLVMA"
     gene            complement(454..1566)
                     /locus_tag="P5335_01770"
     CDS             complement(454..1566)
                     /locus_tag="P5335_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HRZ73637.1"
                     /translation="MKYSYIILDDDTKSILKIQSVMESFSNFTLLGSATQYDDALNLV
                     LEYQPDFVFLEINPISTSSNLSLLLINELHRYLKKTPKIIATSHDSSKAFEAVKYDVL
                     DFLIKPFAISDVRKALLRFEKTIDVPVIRLNPEPRKLQEFKNSEPEEAFLKEKEISNQ
                     PISLKAEPISYTSLNDELFSLLRNEIAGLKEALLEMASSQKSEVNTELIVNQLVKTIE
                     EHFPQDKITVDLEPLLSEIKTISKDSSLNSKVKEEVSRNLICIKSYGDYRFLELDEIA
                     FLKADNNSTDITMNNGEVIIAFKTLKYFEENLPNYFFRIHNSYIVNKNYVSRVHTGNT
                     VCYIKNTKTQLPFSKSYKENVELIIAQLAGSDFKDI"
     gene            1975..2502
                     /locus_tag="P5335_01775"
     CDS             1975..2502
                     /locus_tag="P5335_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HRZ73638.1"
                     /translation="MRKTLFLLICLTGFSSFAQENEIPEFKRNELKGNALFLVLGSFE
                     ATYERLLNEESGIGISVNIPFTNDQDVVFSITPYYRFYFGEKPAAGFFLEGFGMLNQV
                     DDYIYDYNYNYEDINKTITDFAFGIGVGGKWVSKRGVVFEINAGVGRNLFSEYNDNRD
                     YEFIGRGGITVGYRF"
     gene            complement(2574..4115)
                     /locus_tag="P5335_01780"
     CDS             complement(2574..4115)
                     /locus_tag="P5335_01780"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRZ73639.1"
                     /translation="MAKQEDNFKNVISHAKEYGFIFPSSEIYDGLSAVYDYAQNGAEL
                     KKNIREYWWKSMVQMHENIVGIDAAIFMHPTTWKASGHVDAFNDPLIDNKDSKKRYRA
                     DVLIEDYAEKLNQKAQKEIDKAKERFGEAFDEAQFVATNARVMEYKAKQREILERMAR
                     SLGNDDLTDVKALIEELEIACPESGSRNWTEVRQFNLMFGTKIGASADSSMDLYLRPE
                     TAQGIFVNFLNVQKSGRMKIPFGIAQTGKAFRNEIVARQFIFRMREFEQMEMQFFVKP
                     GEEMKWYEYWKETRLKWHLSLGLGRENYRFHDHEKLAHYANAAADIEFNFPFGFKELE
                     GIHSRTDFDLKAHEQYSGKKLQYFDTEENKNYVPYVVETSVGLDRMFLAVFANSLTEE
                     TLEDGSTRTVLRLPSVLAPTKAAVLPLVKKDGLPEISRKIVEELKWDFNVAYDEKDAV
                     GRRYRRQDALGTPFCITVDHQTAEDETVTIRHRDTMQQDRVKIFELRDIITNDVSMRN
                     WLMKM"
     gene            4308..4964
                     /locus_tag="P5335_01785"
     CDS             4308..4964
                     /locus_tag="P5335_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73640.1"
                     /translation="MKNLVNLLIFFSVGILSVSCDDLVDKDDPNEITGAQSEMGLVGV
                     TVTSSSSEIAGVSDFSAVVESLEDGISSYSAQATVTNSLVKNMISNFPGVVIDGNSVS
                     IDNLKIQQTTGGIKCVTGPLAGVLVKYDSSVGDTYTAGTSGNVRTVVSKTGVDDYPYG
                     FYMIKTIQVESTLGNSNRVSGINKITYIANHKFGMVGVKVDFEDGTSYTFPVYTSAEN
                     "
     gene            5055..5507
                     /locus_tag="P5335_01790"
     CDS             5055..5507
                     /locus_tag="P5335_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73641.1"
                     /translation="MKNFLLPHYYKAIGVILVLIAIVFSVLYLKFDLNYTIPVFAIVS
                     IYLETKFFVISQSNFIDEITLLLFVLGFGLIVFSKEKNEFEFLCVLREKALVKATIVN
                     AFIMLFSIIFIYGGGFLGILVLNLFSVFIFYLLFFYVSLKKRNQETRV"
     gene            5571..6254
                     /locus_tag="P5335_01795"
     CDS             5571..6254
                     /locus_tag="P5335_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRZ73642.1"
                     /translation="MLKDLLNLFFPKVCLACKEHLLANENIVCTTCRHHFPLTLHSEN
                     QNNEIIRKFYGRLTLEHASALLYFHKNGIVQELIHNLKYKNRQEIGTLLGEWYAEDLK
                     NIHAELSFDAIIPVPLHKKRMRERGYNQMTTFGQALSKSLQIPLCENILHRDSYAKTQ
                     TKKNFLERTETKKSVFDVDFTEKDHHKHYLLIDDVITTGSTLEQCGKAILKIPGAKLS
                     IVCMAYTHS"
     gene            6336..>6547
                     /locus_tag="P5335_01800"
     CDS             6336..>6547
                     /locus_tag="P5335_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRZ73643.1"
                     /translation="MRVLILIILSVLFASCAKRGTITGGVKDSIPPIMKMSEPKNLST
                     EFKGNKIKLYFDEYIKLKDINKQLIIS"
     assembly_gap    6548..6647
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6648..7382
                     /locus_tag="P5335_01805"
     CDS             <6648..7382
                     /locus_tag="P5335_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73644.1"
                     /translation="PFENKDSLQIWFKPIKADSLSIKVTKNVMEKEFKLKFNKQKADS
                     LRINAVQSGVLHLSEKYTLKSSIPITKIDASKIKLTNKDSVSVVFTTQYDEPKQLVVI
                     DFKKEPLERYKFTLEKGAFTDLLERESDSLAFTIGTKNVTDYGNLRLHLEGIKSFPII
                     VELTNAKGDVQASAYSEGETTIDFNLLQPALFTVRIIYDDNKNKVWDTGSYLEKRQTE
                     EVRYFPTEIDVRANWDVDQTLKFSSE"
     gene            complement(7383..8168)
                     /locus_tag="P5335_01810"
     CDS             complement(7383..8168)
                     /locus_tag="P5335_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRZ73645.1"
                     /translation="MKISLIQAPIVWDNPIANILYFQQKIMEIDEETALIVLPEMFTS
                     GFTMEPFASAENMNGETISWLKKIAETKKVAITGSLIIFENNHFYNRMVYVYPSGKID
                     YYDKRHLFTLAGENKIYEPGTTKKIIKYMGWKICLQICYDLRFPVFARNTEDYDLLIY
                     VANWPETRINAWDILLKARAVENMCYVVGVNRIGSDFNKLEYVGHSQVIDELGNFLVE
                     PQVEEGIFQAVLDMKKLLNTRKKLNFLADRDEFEIKLTKKKAE"
     gene            complement(8173..>8463)
                     /locus_tag="P5335_01815"
     CDS             complement(8173..>8463)
                     /locus_tag="P5335_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HRZ73646.1"
                     /translation="TANFLQDTRSWILMPTKVEYYVSEDNVNFTLFDTQNNTIDPKEY
                     KTIIKGVESIQLPVKARYVKVKATNFGKLPEWHQGFPSGGDAFIFIDEIIIK"
     assembly_gap    8464..8563
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8564..10760)
                     /locus_tag="P5335_01820"
     CDS             complement(<8564..10760)
                     /locus_tag="P5335_01820"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HRZ73647.1"
                     /translation="MRFFIFDVKKLFMRLLIISFSMFFTGLIVGQNYQQYVNPFIGTG
                     GTGHTFPGATLPYGMVQLSPDTRIDGSWEGCSGYHYSDNLIYGFSHTHLNGTGVSDYG
                     DILLMPTMGEPSFNNKIYSSTFSHANEKASAGYYSVKLDKHNIDVQLTTSTRVGFHEY
                     TFNQSGQANIILDLNHRDKLLEGEIRIIDKKTIFVKRRSEAWARDQYVYAFIEFNQPL
                     EINKVNYNGKQFGRLFNGTELAISFSKKVKRGDKLLVKVTLSPTGYEGAQGNSTEIPH
                     WNFEKTKKAAEELWNKELSKIEITSTDKNKLTIFYTALYHTMMQPNIAQDIDGKYRGR
                     DNKIHQAEGFDYYTVFSLWDTFRAAHPLYTLIDKKRTSDYINTFINQFEQGGRLPVWE
                     LASNETDCMIGYHSVSVIADAMVKGIKGFDYEKAYEAAKYSAMLDHLGLDAYKKQGYI
                     SIDDEHESVSKTLEYAYDDWCIAQMAQLLDKKEDYQYFMLRSQNWKNLFNWETGYIQP
                     KKNGGWDKPFDPKEVNNNYTEANGWQYTFFVPQDILGLADAIGGPTHLELKLDEMFNS
                     VSETSGRHQVDITGLIGQYAHGNEPSHHVAYLYSFLGSPEKTTGKVHFILDNFYKNDP
                     DGLIGNEDCGQMSAWYVLSSMGMYNITPGQSDWTLSKPYFENIKVHFENGEYGEKSSN
                     KEHDSDRFFGFWNNYRIRNDYKYKELITVPVIQAESKTFKDKMTIKIQSN"
     gene            complement(10802..11494)
                     /locus_tag="P5335_01825"
     CDS             complement(10802..11494)
                     /locus_tag="P5335_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRZ73648.1"
                     /translation="MKKLVYIMLFLPLLCLGQMETNGTIKAELVTKFELNYLLQKPNN
                     NKDLKPLIIFLHGSGEKGTDLEKLKIHGPLKYAKTHELDAYILAPQCPENKYWESEEL
                     YLLIQKVLAENKIDKSRIYLTGLSMGAWGSWNLAFAHPELFAALVPIAGFVDRVPMVE
                     NCKISTIPIRIFHGLQDDIVDANYSIAIFNKLRGCNKDIKLQLFDDANHDSWTRVYDN
                     PAIYEWMLMQKK"
     gene            complement(11499..13454)
                     /locus_tag="P5335_01830"
     CDS             complement(11499..13454)
                     /locus_tag="P5335_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRZ73649.1"
                     /translation="MKFKWHFLLLITFISSFALEAQVKVAAIFSDNMVLQRHSKIPVW
                     GWANANEKVTVQFHNQTKTTKADQTGKWIVRLDDEMAGGPYVLKIKGKNSIEIKNILV
                     GEVWICSGQSNMEWTVGQADEAEKEIDMADFPQIRHVKIPKEINSFPASDIQKTAWEV
                     CSPKTVADFTAIGYYYAKELNNKLKIPIGIINASWGGTNIETWISREGFELDSEFRDM
                     IYSMPKIDIHSLLDAKMDAAKTRIEKIQNSKLNPEDVSFYKDLNFKDSNWSEIHQPGL
                     WEEQQLGNFDGVIWLRKHFTLSEITENCIIEIPAIDDNDITFINGIKIGQTNGWDEKR
                     MYKIPSGILKLGDNVITIRVVDNGGGGGIYGNAAALKIILNNKEIPLDGYWKFQVESI
                     KNSINENDFPSLCFNSMIHPLIPFAFKGVLWYQGESNVSRAFQYRKTFPMLISDWRKK
                     WKNDFPFYYVQLATYQTTGNSNEGCAWAEIREAQTKTLSVKNTGMVVTTDVGNPNDIH
                     PTNKKTVAKRLASIAFTNLNNENMISNGPTFNSFEKKEKQLIVTFNSLGAGLVTTNEY
                     GFVNGFEIAGENQVFYPAKAYIKENKVVVSNENVLNPIAVRFGWIGDASECNLFNVEG
                     FPAVPFRSDEWKTITKEEKYKIDIIVN"
     gene            complement(13444..14676)
                     /locus_tag="P5335_01835"
     CDS             complement(13444..14676)
                     /locus_tag="P5335_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HRZ73650.1"
                     /translation="MKKVFTNLTTWVLIAILLGAILGHFLPETAIQLQPLGTYFINIV
                     KVFINPIIFLTISLGISGMGSLKKVGRVGGKALLYFEIVTTLALLIGVVVTWIIQPGI
                     GIETISLQPAKISKYTTGAETFGLWNFIKSNLTIQVLLFSILFGTFLNKYKHSDRIKK
                     YLSFASKYVFKALHLVMLFAPLGALGGIAYTIGKFGIATMIPLAKLMITVYVTMALFI
                     FIVLGAILKYHKISILNFLNYIKEELLIVLGTSSSESALPSLMEKLEKMGCSKSVVGL
                     VVPAGYSFNLDGTTIYLSMAILFLAQVYNIDLSWTQMGTIIGVLMITSKGAAGVTGSG
                     FIVLASTLSAVKVIPIEGLALLLGVDRFMSEARSITNFIGNGVATIFLANNENEFERL
                     KMEIAFTKEVYEYEKHEI"
     gene            complement(14791..17016)
                     /gene="bglX"
                     /locus_tag="P5335_01840"
     CDS             complement(14791..17016)
                     /gene="bglX"
                     /locus_tag="P5335_01840"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HRZ73651.1"
                     /translation="MKKKVIFLFIGFQSFVAFSQQKSINQKVEDLLKQMTLEEKIGQL
                     NQYTGDNTVTGPLTINPNKKEEIKSGKIGSMLNVMGSTYTRQYQEYAIQSRLKIPLLF
                     GLDVIHGYKTTFPIPLAEAASWDLSAIELGARIAATEAAASGIHWTFAPMVDIGRDPR
                     WGRVMEGAGEDTYLGSKIAFARVKGFQGEKLGELNAVMACAKHFAAYGAAVGGRDYNS
                     VDMSERMLWETYLPPFKAAIDAGAATFMNSFNDINGIPATGNKYLQRDILKEKWGFKG
                     FVVSDWGSIGEMIAHGYSKDAKEAALQAITAGSDMDMESNAYRFNLEQLVKEKKVSLA
                     LIDDAVKRILHKKFELGLFDDPYRFCNLERQNQALNNPEHTKIARDIAKKSIVLLKNE
                     INVLPLSKSIKSIAFIGPMVKPKRINHGFWAVDLKEIDSTYIVSQWDGLKNKVGKNTK
                     LLYAKGCGVENTSKEGFQEAVDIASQADVVILSIGEAWNMSGEAKSRSNIHLPGVQED
                     LVKELQKTGKPIVILINAGRPLIFNWIADNMPTIVYTWWLGSEAGNAIADVLFGDYNP
                     SGKLPMTFPREEGQIPIYYNHFNTGRPSVNEDKIYKSAYIDLPNSPKFPFGYGLSYTS
                     FAYSDLKLSKQKMKSNETIEVSFTLTNTGKFIGEEVVQLYLRDKVGSVVRPIVELKDF
                     NKIKLEVGETKTIKFSIDNQKLSFYNEKLVFDSEIGDFDIMIGSSSTDIRLKGRFELV
                     K"
     gene            complement(17027..18469)
                     /locus_tag="P5335_01845"
     CDS             complement(17027..18469)
                     /locus_tag="P5335_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HRZ73652.1"
                     /translation="MKILLSTLMVSSVLFGQTKDKINSNLPNHQSVTVYTTAPDSNLK
                     LAKTDVLTFKEYKQPLETQLCVFVHPNKTNQTFLGIGGAITDASAEVFAKLPLTKQNE
                     FLDAYFDKEKGIGYTLIRTNIHSCDFSSESYTYVEEGDKELKTFSIEHDRAFKIPLIK
                     KAMEKAGKATFYASPWSPPAFMKDSKNMLKGGKLLPEFYQSWANYYVKFVQAYQKENI
                     PVWGLTIQNEPMATQIWESCIFTGDEEREFLKNYLGPTLQKNGLNDVKITVWDHNRDL
                     LSQRASAVLDDPEAAKYVWGIGFHWYENWSGGNPMFENVAKVHQMYPDKNLLFTEGCV
                     EKFSAEKYQFWANGQRYGRSMINDFNNGTVGWTDWNILLDQNGGPNHVGNFCFSPIHG
                     DTSTGELIYTPSYYFIGHFSKFIKSGAKNFSCTSSRSQLIVTSFKNPTGELVTIVMNE
                     SDKELSYLLSNNNQVSEIKIPAKAIQTLVY"
     gene            complement(18487..>19320)
                     /locus_tag="P5335_01850"
     CDS             complement(18487..>19320)
                     /locus_tag="P5335_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HRZ73653.1"
                     /translation="SLQPQYYSVYANYFVKYIQAMQAEGIIIDAITPQNEPLHPGNNP
                     SMYMTATQQADFIKNHLGPAFQTASITTKIIIYDHNCDKPEYPIQVLNDASAKSYIAG
                     SAFHLYGGDISALSIVKNAHPTKDLYFTEQYTSNTGNFDGDLQWHVKNVVIGSMRNWS
                     KIALEWNLANNASFGPHTPGGCTTCKGAITINSSSSFAKNVGFYIIGHASKFVPEGSI
                     RIGSSVVGNLYNVAFKRPDGKKVVIVVNDGNNSELFNIKYNGKWITTSLDAGAVGTYT
                     W"
     assembly_gap    19321..19420
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19470..20303)
                     /locus_tag="P5335_01855"
     CDS             complement(19470..20303)
                     /locus_tag="P5335_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRZ73654.1"
                     /translation="MKKSILLCCFLIPLIGISQDLKLMTYNIRLDVASDGENSWANRK
                     DFFTAQIKFYEPDIFGIQEATPNQVIDISNELTQYNQVGIGREGEGKGESSNIYFKKE
                     RFKVITENTFWLSETPNQISKGWDAAFYRVCTYVLLKDTKKNILFWVFNTHLDHVGEI
                     ARTKGIELILKQIEKVNTKNYPVFFMGDFNSEPSTERIISLQSKMNDSREIAIQKPFG
                     PTGTFNGFKHNEPVTILIDYIFVSKNNTLKINKYAVLSDSKDLKYPSDHLPVFIEVNY
                     K"
     gene            complement(20432..21508)
                     /locus_tag="P5335_01860"
     CDS             complement(20432..21508)
                     /locus_tag="P5335_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HRZ73655.1"
                     /translation="MKICTAFKKNLYLFMTLFCLPILGCSNDSSDGGDTITPSNLAVT
                     ITKVGFSDSTPNGDGSGTISLTATAVNATLYKINFGNGQLVESTTGLVNHTFTDMGTN
                     TYTIYVSAYNGTKFISKTIATTVYVSPELLWSDEFNVNGAPNSTYWGYDIGTGSNGWG
                     NNEAQYYTNRPENVIVQDGILKINLIKEVYSGSNYTSARIVTKDKFSFKYGRVEIKAK
                     LATGGGTWPALWMLGDNIGTAGWPGCGEIDIMEHVGNAQNKIYGTLHHPGHSGGNADG
                     GNVTISNASSEFHVYAVDWTSSYIKFYVDNQLYYSFVNTNSLPFNQNFFLIFNCAMGG
                     NFGGNIDPNFTSTTLEVDYIRVYQ"
     gene            complement(21570..22505)
                     /locus_tag="P5335_01865"
     CDS             complement(21570..22505)
                     /locus_tag="P5335_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73656.1"
                     /translation="MKKNFKYIFALVFMGAIFFACNNDEDYSLGDLDAPTNLVIATEI
                     VGQDINNPEGDGSGDVKITITADNALSYKVDYDANNDVDLVYLPTGKVTKKYTSLGVN
                     TYRITAVAYGKGGSSTIITKDITVRSDFNVNPEIVTNLTNNSSKKWIVDKSVPGHFGV
                     GPWANSSTPEWWSAAIDEKVACCNCFYTATFTFSKTVSNTYTLDVVTSDGAFTKTGSL
                     ASIPGIPASGDEGCYPYAGGSSSFSFVPSSTGIDASTPSTQTSILLSGTTTFIGYGSL
                     QKEYEIMVLDGNYMYLRVQGTETGNAWYLKLKPAP"
     gene            complement(22518..23621)
                     /locus_tag="P5335_01870"
     CDS             complement(22518..23621)
                     /locus_tag="P5335_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73657.1"
                     /translation="MKKIKFLICFVLLQTVVIGCSEEDGIGTDLSSLNSSEPTATSKI
                     FDISDDNSGNVRITPFGEGVTEFTVNLGHGDGELVSLSPGNSVTHSYPEGSYTVTIVS
                     RGITGIEVENTYPLQLVYRAPENLVINKNLAGYDLTVSATADYANGFLVYFGDVTNEV
                     GTPMAVGETLPPHTYAAPGVYNVKVIALSGGAAQSELTESITIYAPFSLPITYESPIQ
                     NYNIGGTFGDVSVSQIANPNPGGINTSATVRKYIKAVGAPGWGGTWTPLSTPNGTPIN
                     IDNGSIIKVMVYSTEVGKMLNVELEQGSDGVSNKILKVASTVANQWEELSFDFGTFGI
                     PAGTTFNQLVFRYNDTANGTGEVIYLDNIKQTN"
     gene            complement(23702..25213)
                     /locus_tag="P5335_01875"
     CDS             complement(23702..25213)
                     /locus_tag="P5335_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRZ73658.1"
                     /translation="MKHKFKLVIVSAFSIGLAVSCSSDFLEVEPKGLALESNYYQNAD
                     EAYAGLVAVYDVMRKNSGGFENMITMFNAGSDDHVAGGGGATDGVGIQSFSNYTLDSN
                     TIPASFWNDYYQGIFRANVLLSKIPNIPMGDDLKVRFTAESKALRAYYYFNLVRMFKN
                     IPLILNPLSPTEIYSIPQANPADVYAQIEQDLIDAIADLPATVSLSTEAGRFTKGSAQ
                     ALLGKVYLYQGGKNALAAEQLQEVNGTPGGTSQYGYKLLDNFNDLWVVTNKFNTESIL
                     EVTHTSQGNSYWGIWGGGQDEGNTVNVMVGPRGYTAIGSTAPALPSGWSFNVFTQDFY
                     DFMADDPRFGATVLDLKALKLAGEVDYIGGYEDTGYFLNKFLPRQADAHTGAGDAVLN
                     YKQNTYAIRLADTYLMEAEALGGSGTRAQALLDAVRSRVGLPSVPVSMTTIMDERRRE
                     LAGEGHRWFDLVRTGQAASKLASRGFVAGKHEILPIPFKELENTLLVQNPSYN"
     gene            complement(25226..>27763)
                     /locus_tag="P5335_01880"
     CDS             complement(25226..>27763)
                     /locus_tag="P5335_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRZ73659.1"
                     /translation="GFVNQADIESMEVLKDAASLAIYGARAASGVILVTTKKGKQGKM
                     TLNYNGFYGVSSPAKMLNLLNATEYGAIMNEKAVAAGQNLVFPDLSTLGVGTDWQKQI
                     FNNNAQRNSHEVSVGGGGEISTFFLSFGSSDQEGIVATEISNFNKKNFRINSTHKFKE
                     WVTIGQTLGYTHQKGIGLGNTNSEYGGPLSSAINLDPTTPVVVTDPAIANAAPYNNNP
                     VIRDANGNPYGISSLVGQEMTNPLAYIQTRLGQYGWSDDFVGNIYFDFKLHKDFKFRT
                     TLGGKLAYWGAEGFTPEYYLNASTLTSQNNISRRNNKSFGWNIENTLTYTKQIEKHNI
                     TVLLGQGAYQDNKIYTETGVTYFDIPVTNYQDASFNFDVPASQRSSYSVDGVEHNVNS
                     LFARVNYDYNEKYLFNGVIRRDGSSRFGQNNQYGVFPSFSVGWVASNEDFWKENKVVT
                     NLKIRAGYGVTGNDAIPDFGYLSTIGGGRNYTIGDAGNITSGYSPNAPSNPDLKWEET
                     AQTNIGFDAKIYNDFNFTFEWYKKVTTGILQYQQIPGYVGATGQPLANIADMENSGLE
                     FELGYKKKFGDFNFSVNANYSTLNNEVTHLGNGIEYITNGSAGFQSMGPITRTQVGEA
                     YNSFYGYQTAGIFQNQDEINAYTNGDGGLIQPDARPGDFRWTDSNGDGTINDEDRVFL
                     GSSLPTYNYGFTINLDYKGFDFMAFAQGAGGNKIFQGLRRLDVGNANYQTDALGRWTG
                     EGTSNSYPRLTNNDTNGNFGKPSDFYLEDGDYLRLKLIQFGYSLPNDVIGKVGLQKLR
                     FYVTGENLLTFTKYTGYDPEIGGSVFGVDRGYYPQARSIMFGANLQF"
BASE COUNT         8667 a         5040 c         4280 g         9476 t
ORIGIN      
        1 gccataacta aatctctctt tttagaagga cttgttgaat actctcctgt ttcagtagtc
       61 attactaaat ctcttttttt agatggacta gttgaatact ctcctgtctc cattaactga
      121 ggttcgttac ttgttattgt gctttcagtt aaatctgttg aagaaacaga agcaaacata
      181 aacaatccta aaaatgcaaa taatgtcttc atggtatttt gtgttttaag ttatttcgta
      241 aataaaattt ttggtgctat tctttaataa tggtatgcta ttcttattaa ctgtgattat
      301 gcataaagat ggctgcctaa cgtggggaca cctttcattc acgatgtaaa agtatagtgc
      361 atctctcgta cagccaataa aatcaagggg tgaatggtga gtgtaatccg tttaggtgtg
      421 gattggtatt aaatcttggt agaatagagt cttttaaatg tctttaaaat ctgaccccgc
      481 taattgggca ataataagtt caacattctc tttataactc tttgaaaacg gaagttgtgt
      541 tttcgtgttt tttatgtaac aaaccgtatt tcccgtgtgg actcttgaaa cataattttt
      601 gttgacaata tagctgttgt gaattctaaa gaagtaatta ggcagatttt cttcaaaata
      661 ttttaatgtt ttgaatgcta taatgacttc tccattattc atggtgatat ccgttgaatt
      721 attatcagcc ttcaaaaaag caatttcgtc taactctaaa aagcgataat caccatacga
      781 ttttatgcaa attaaattcc ggcttacttc ttcttttact ttgctattta aagaagaatc
      841 ttttgaaatc gttttaattt ctgaaagcaa tggttctaaa tcaaccgtga ttttatcttg
      901 agggaaatgc tcttcaatcg tttttacaag ttgatttaca ataagttccg tgttcacctc
      961 tgatttctgg ctactagcca tttccaaaag tgcctcttta agtccggcta tttcatttct
     1021 taaaagcgaa aataactcat cgtttagtga tgtataagaa attggttccg cttttagtga
     1081 aattggttgg tttgatattt ccttctcctt taaaaacgct tcttccggtt cactattttt
     1141 aaactcttga agtttacgtg gctcaggatt taaacgaatt acgggaacat caatggtttt
     1201 ttcaaatcgc aataaggctt tacgaacatc gctaattgca aatggtttaa tcaaaaaatc
     1261 caacacatca tattttaccg cttcaaatgc tttggaagaa tcatgtgaag tggcaataat
     1321 ttttggtgtt ttttttaaat atcgatgtaa ttcattgatt aacaacaaag aaagattgga
     1381 agaggtggaa attggattga tttccaggaa aacaaaatca ggttgatatt ctaagactaa
     1441 gtttaaagca tcatcatact gtgtagctga gcctaaaagt gtaaaatttg aaaaactttc
     1501 cattacggac tgtattttca aaatactttt agtatcatcg tcaagaatga tatatgaata
     1561 cttcataaac ctatcaaatc aaatcaaaaa cggaggaagc accaacaacc tttcgttctg
     1621 ttttttgtta tattatgctg atttgtaggt tatttgcgta aaaataaggg gcagttttac
     1681 actttaacaa ttttacttat aataatcggg gtatttttgt gtaaatcatt ataattatta
     1741 acaaacataa gttaaaaaaa gtccttttaa aagtaatttt ctaacgtttc gataacacgc
     1801 aaaaatttcc gattaaaaac tagtataaca tttttcacta aaaattaaat ccctagaaaa
     1861 gcgtcattga atgaaataaa ttaaatcaaa aaaataaaga attttcacag acttttatac
     1921 gaaaaatggc atgttatttg ttactttcgt aaaaaaaaat aaatcaatta aattatgaga
     1981 aaaacacttt ttttacttat ttgcctgacc ggcttttcgt cttttgcaca agaaaatgaa
     2041 attccggaat tcaaaaggaa tgaattgaaa ggaaatgctt tatttttagt tttggggagt
     2101 tttgaagcta cttatgaacg attattaaac gaagaatcag gaattggaat tagtgtaaac
     2161 attcctttca ccaatgatca agatgtcgtt ttttctatta ccccttatta tcgtttctac
     2221 tttggtgaaa aaccggctgc cggatttttt ttagaaggtt ttgggatgtt aaatcaagtg
     2281 gatgactata tttatgatta taactataac tatgaagata tcaataaaac cattaccgat
     2341 tttgcctttg gtattggcgt aggaggaaaa tgggtttcaa aaagaggcgt tgtttttgaa
     2401 ataaatgccg gtgtagggag aaacttattc agtgaatata atgataatcg cgattatgaa
     2461 tttattggaa gaggtggtat aactgttgga tatagattct aaacaataaa ttaaaagccc
     2521 aaatttcaat tactttaaat ttgggctttt tttttatttc aaattttgaa aacttacatt
     2581 ttcatcaacc aatttcgcat cgaaacatca tttgtaatga tgtctcttag ctcaaaaatt
     2641 ttcacgcggt cttgttgcat ggtatcacgg tgacgaatag taaccgtctc atcttctgcc
     2701 gtttggtggt caaccgtaat gcaaaaaggt gtgcccaacg catcttgcct tctgtaacga
     2761 cgacccacag catctttttc atcatatgcc acgttgaaat cccacttcaa ttcttctaca
     2821 atctttcttg aaatttcagg caacccgtct ttcttgacta aaggtaaaac agccgcttta
     2881 gttggtgcta aaaccgatgg taaacgtaaa actgttctcg ttgaaccatc ctctaacgtt
     2941 tcttctgtta atgaattggc gaaaacagcc aaaaacatac gatccaaacc aactgaagtt
     3001 tctaccacat atggaacata atttttattc tcctctgtat caaaatattg caattttttg
     3061 ccagaatatt gttcgtgggc tttcaaatca aaatctgttc tcgagtgaat tccttctaat
     3121 tctttgaatc caaaaggaaa attaaattcg atatccgctg ccgcattggc ataatgagcc
     3181 aatttttcgt ggtcgtgaaa acggtaattt tctctaccta aacctagcga taaatgccat
     3241 tttagacggg tttctttcca gtattcatac catttcattt cttcccccgg tttaacaaaa
     3301 aactgcattt ccatctgttc aaattcacgc atacgaaaga tgaattgacg agctacaatt
     3361 tcgtttctaa aggcttttcc ggtttgagca attccaaatg gaattttcat acgacccgat
     3421 ttttgtacgt ttaagaaatt tacaaaaatt ccctgagcag tttcaggacg taaatataaa
     3481 tccatagaag aatcggcaga agcccctatt tttgtcccaa acatcaagtt aaactgacgt
     3541 acttctgtcc aattccttga tcctgattcc ggacaagcaa tttctaattc ttcaattaac
     3601 gcttttacat cggttaaatc atcattcccc aaagaacgtg ccattctttc cagaatttca
     3661 cgctgtttag ctttgtattc catcacacgg gcattagttg ccacaaattg tgcctcgtca
     3721 aaagcttctc caaaacgttc tttggctttg tctatttcct tttgtgcttt ttgatttaac
     3781 ttctcggcat aatcttcaat taaaacatcg gcacggtatc ttttttttga atctttgttg
     3841 tcaatcaatg ggtcgttaaa cgcatccacg tgaccggaag ctttccaagt ggtgggatgc
     3901 ataaaaatcg ccgcatcaat tcctacgatg ttttcgtgca tttgcaccat tgatttccac
     3961 caatattctc tgatattttt ctttaattct gctccgtttt gagcatagtc gtataccgca
     4021 cttaaaccgt cgtaaatttc actcgacgga aaaatgaatc cgtactcttt tgcatgtgaa
     4081 attacgttct taaaattatc ttcttgtttt gccataatga tgcaaaaata taaaaagtga
     4141 atagataaaa aagagaagaa ttgcttttat ttttttaagt taatttaatt gatgtaaaca
     4201 ttctttttat gaggaattta ttaggaattt attaggtttt aatttgataa tttcttaact
     4261 ttaatagtga taaagttgat atttcataat tattaaaaat atatatcatg aaaaatttag
     4321 taaacttgct tattttcttt tctgtaggaa ttttaagcgt atcatgtgat gatttggttg
     4381 ataaagatga tcccaatgaa ataactgggg ctcagtctga gatgggttta gttggtgtaa
     4441 ctgtaacttc aagttcgtct gaaattgctg gtgtaagtga tttttcggca gtagtagaaa
     4501 gccttgaaga cggaatttca agctatagtg ctcaagctac tgtaactaat tcgttagtga
     4561 aaaatatgat ttccaatttc cccggcgtgg taattgacgg caattctgtt tctattgaca
     4621 atttaaaaat ccaacaaaca acaggcggta ttaaatgtgt taccggtcct ttagcaggcg
     4681 ttttagttaa atatgattct tccgttggcg atacctacac tgccggaaca tcaggaaacg
     4741 ttagaaccgt tgttagtaaa accggtgttg atgattatcc ttatggtttt tacatgatta
     4801 aaactattca agtggaatca acattaggca attcaaatag agtgagtgga atcaataaaa
     4861 ttacgtatat cgcaaatcat aaatttggaa tggttggtgt aaaagttgac tttgaagatg
     4921 gcactagcta tacattcccg gtttacacca gtgctgaaaa ttaacaattt aatacattgt
     4981 ttttcgatta ggtaaggttt tctgccttgc ctaatcgaat aaagatttaa ttctcttact
     5041 atcttaaaat tgaaatgaaa aatttccttt tgccccatta ttacaaagcc attggcgtta
     5101 ttttggttct tattgcgatt gttttttctg tactttattt aaaatttgat ttaaattata
     5161 caatacctgt atttgcaata gtttcgattt atttagaaac taaatttttt gtaatatccc
     5221 aaagcaattt tatagatgaa attaccctcc ttctttttgt attagggttt gggcttattg
     5281 tgttttctaa ggaaaaaaat gaatttgaat tcttatgcgt tttgagagaa aaagcactgg
     5341 taaaagcaac aatagttaat gcttttatta tgttgttttc tataattttc atttatggcg
     5401 gtggattttt gggcatattg gtgttgaatt tattttccgt ttttatcttt tatctactct
     5461 ttttctatgt aagcttaaaa aaaagaaatc aagaaactcg tgtgtaattg aataattata
     5521 aatttaatgt atctttattt tgaaaataaa gacattgatt tataattttt atgctaaaag
     5581 atttactcaa tttattcttc cctaaagttt gtttggcttg taaagaacat ttgttagcca
     5641 atgaaaatat agtttgtaca acttgtcgtc atcattttcc gctaacactt catagtgaaa
     5701 atcagaacaa tgagattatt cgaaaattct acggccgttt gactttagag catgcttccg
     5761 ctttacttta ctttcacaaa aacggcattg ttcaggaact tattcacaat ttaaaataca
     5821 aaaaccggca ggaaattggt actttattag gagaatggta tgctgaagac cttaaaaata
     5881 ttcatgccga actttccttt gatgccatta taccggttcc tttacacaaa aaacgaatgc
     5941 gagaacgcgg ttacaatcaa atgactactt ttggtcaggc tctctcaaaa tcgcttcaaa
     6001 tacctttgtg tgaaaacatt ctacacagag atagttatgc taaaacacaa accaaaaaga
     6061 attttctgga acgaacggaa actaaaaagt cggtttttga tgttgatttt acagaaaaag
     6121 accatcacaa acattatctc ttaattgatg atgttattac cacaggatca acgctggaac
     6181 aatgtggaaa agcaattttg aagatacccg gagctaaatt aagtattgtt tgcatggcat
     6241 atactcattc ataaaacgca aagaaaaacc taaaacattt catttccact aaatataatt
     6301 gttattttgt ggcaacgaag aaagcatttt atttcatgag agttttaatc cttatcattt
     6361 tatcggtttt gtttgcaagt tgtgcaaaaa gaggcactat tactggcgga gtgaaagatt
     6421 ctattccacc aatcatgaaa atgagcgagc ctaaaaattt atctaccgaa tttaaaggaa
     6481 ataaaataaa attgtatttt gatgaataca tcaagctgaa agacatcaac aaacaattga
     6541 ttatttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncca tttgaaaata
     6661 aagattcatt acaaatttgg tttaaaccaa ttaaagctga ttcactttca ataaaagtga
     6721 ctaaaaatgt gatggaaaaa gaatttaaac taaaatttaa taaacaaaaa gcagattcgc
     6781 tacgtattaa tgccgttcaa agtggcgttt tacatctaag cgagaaatat accctgaaaa
     6841 gttccatccc gattacaaag attgatgcat caaaaatcaa acttacaaat aaagattctg
     6901 tttccgttgt ttttacgacg caatatgatg aaccaaaaca acttgtcgtt atcgatttta
     6961 agaaagaacc ccttgaaaga tataaattca cccttgaaaa aggtgcattt actgatttat
     7021 tagaaagaga aagtgactca ctagcattta caattgggac caaaaacgtg acggattatg
     7081 gtaatttacg actgcattta gaaggtatta aatctttccc catcatcgtt gaattaacca
     7141 atgcaaaagg agatgtgcaa gcgtccgcct attcagaggg tgaaacaacg attgatttta
     7201 atttactcca accggctttg tttaccgtac gaatcatata tgatgacaat aaaaacaagg
     7261 tttgggatac cggaagttat ttagaaaaac gacaaacaga agaagtacgc tatttcccaa
     7321 cagaaattga tgttcgtgca aattgggacg ttgatcaaac gctgaaattc agtagcgaat
     7381 aattattcag cctttttttt ggttagttta atttcaaatt catctctatc agctaaaaaa
     7441 tttaactttt ttctcgtatt caaaagcttc ttcatgtcta aaactgcttg aaaaattcct
     7501 tcttctactt gtggttccac caaaaaatta cctaattcat caattacttg ggaatgtcca
     7561 acatattcca atttattaaa atcggaacca atacggttaa cccccacaac ataacacatg
     7621 ttttcaacag ctcttgcttt cagtaaaata tcccaagcat tgattcgagt ttccggccaa
     7681 ttagcaacat aaatcaacaa atcgtagtct tctgtatttc tagcaaaaac agggaatcgc
     7741 aaatcataac aaatttgaag acatattttc caacccatgt atttaatgat ttttttagtt
     7801 gttcccggtt cataaatttt gttttctcct gccaaagtaa ataaatgtcg cttgtcataa
     7861 taatctattt taccggaagg ataaacataa accattcggt tataaaaatg attattttca
     7921 aaaatgatta aactcccggt tatggccact ttcttcgttt cggctatttt ttttaaccag
     7981 gaaatggttt ctccattcat gttttctgct gaagcaaatg gttccattgt aaaaccggaa
     8041 gtaaacattt ccggcaaaac aatcaaagca gtttcttcat caatttccat tattttttgc
     8101 tgaaaataca agatgttcgc aattggatta tcccaaacta ttggagcttg aataagtgaa
     8161 attttcatgt ctttatttta ttattatctc atctataaaa ataaaagcat cacctcccga
     8221 gggaaaacct tgatgccatt cgggtaattt tccaaaattg gttgctttaa cttttacata
     8281 acgagctttt acaggcaatt gaatgctttc aaccccttta atgatggttt tgtattcttt
     8341 cgggtcgatg gtgttatttt gcgtgtcgaa aagcgtaaaa tttacattgt cttccgaaac
     8401 ataatactcg acttttgttg gcatcaaaat ccacgatcgg gtgtcttgta gaaaattggc
     8461 ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncattcga ttgaatttta
     8581 attgtcattt tatccttaaa agttttactt tcagcttgta taacagggac tgtaattaat
     8641 tctttatact tgtaatcatt tcgaattcga taattattcc aaaaaccaaa aaaacgatct
     8701 gaatcatgtt ctttgttgct gcttttttct ccatattcac cattttcaaa atggactttt
     8761 atattttcaa aataaggttt agataaagtc caatcagatt gtcccggagt tatattatac
     8821 attcccatcg aactcaacac ataccacgca ctcatttggc cgcaatcttc attgccaatt
     8881 aagccgtcag gatcgttttt gtaaaagtta tctaaaatga aatgtacttt tcccgttgtt
     8941 ttttctggtg accctaagaa actatacaaa tacgccacat gatgacttgg ttcgtttccg
     9001 tgagcatatt gcccgattaa tccggtaata tcgacttgat gacgaccaga cgtttcgcta
     9061 acactgttaa acatttcgtc tagtttaagt tctaaatgtg tcggcccacc tattgcatct
     9121 gctaaaccta atatatcttg tggcacaaaa aacgtgtatt gccaaccgtt ggcttcggtg
     9181 taattgttgt tcacttcttt tgggtcaaaa ggtttatccc aaccaccatt tttcttaggt
     9241 tgaatgtatc cggtttccca attgaaaagg tttttccaat tttgagaacg aagcatgaaa
     9301 tactggtagt cttctttttt gtctaataat tgagccattt gagcgataca ccaatcgtca
     9361 tacgcatatt ctaaggtttt ggaaacactt tcatgctcgt catcaatcga aatataacct
     9421 tgttttttgt aggcatccaa ccccaaatga tccaacatag ccgaatattt ggcggcttca
     9481 taggcttttt cgtaatcaaa acctttgatg cctttgacca tcgcatcggc aatcacggaa
     9541 acggaatggt agccaatcat acaatcagtt tcgttggagg cgagttccca aacgggtaag
     9601 cgaccgcctt gttcaaattg attgataaag gtgttgatgt aatcggatgt tcgcttttta
     9661 tcaatcaacg tatacagcgg atgagcggct cgaaacgtat cccacaggga aaaaacagtg
     9721 taataatcaa atccttcggc ttggtgtatt ttgttgtcgc gacctcggta ttttccgtct
     9781 atatcctgag caatgttagg ttgcatcatg gtgtgataca aagcggtgta aaaaatagtg
     9841 agtttgtttt tatcagtaga ggtgatttca attttggaga gttctttgtt ccataattct
     9901 tctgctgctt ttttagtttt ttcgaaattc caatgtggta tttcggtgct gttgccttgt
     9961 gctccttcat atcctgtggg tgaaagagtt acttttacga gtaatttgtc tcctcttttg
    10021 acttttttgg aaaaactgat ggctaattcg gttccgttaa aaagccttcc gaattgtttt
    10081 ccattataat ttactttatt aatttctaat ggttggttaa attctataaa agcataaaca
    10141 tattggtcgc gagcccaagc ttcgcttctt cgcttgacga aaattgtttt tttatcaatg
    10201 attctaattt ccccttccag caatttatca cggtgattta agtctaaaat aatgttggct
    10261 tgaccacttt gattgaaggt atattcgtga aaacctactc ttgtggaggt agttaactgc
    10321 acatcgatgt tgtgtttgtc taatttaact gaataatatc cggcagaagc tttttcgttg
    10381 gcatgtgaaa aggtggatga atagattttg ttattaaagg atggttctcc catggttggc
    10441 atcaataaaa tatcgccata atcggaaacg ccggttccgt tgagatgggt atgtgaaaaa
    10501 ccataaatca aattatcgga ataatgataa cctgaacagc cttcccaact gccgtcgatg
    10561 cgggtgtcag gggaaagttg caccattccg taaggcaacg tagctccggg aaatgtgtgt
    10621 ccggttccgc ctgttccgat gaatggattt acatattgct ggtagttttg gccaacaatt
    10681 aagccggtaa aaaacattga aaaagaaatg atgagcaatc gcatgaataa ttttttcaca
    10741 tcaaatataa aaaaacgcat ccaacaattg ccgaatgcgt attaaataac ttataaaaac
    10801 actatttttt ttgcattaac atccattcat aaattgcagg attatcataa acacgcgtcc
    10861 aactatcgtg attagcatca tcaaagagtt ggagtttgat atctttgtta catcctctca
    10921 atttattgaa aatagcgatg gaataattcg catctacaat atcatcttgt aatccgtgaa
    10981 aaattcgaat gggaatcgtt gaaattttac aattctctac cataggtact cgatcaacaa
    11041 aaccggcaat tggtactaag gcggcaaaaa gttcaggatg tgcaaacgct aaattccaac
    11101 ttccccatgc tcccatactt aatccggtga ggtatattct ggatttgtct attttgtttt
    11161 ctgccaacac tttttgaatt aaaagatata attcttctga ttcccaatac ttgttttccg
    11221 gacactgcgg agctaaaata tatgcgtcca gttcatgtgt tttggcatat tttaaaggac
    11281 catgaatttt taatttttct aaatccgttc ctttttcacc tgagccatgc agaaatatga
    11341 tgagcggttt taaatccttg ttgttatttg gtttttgaag caaataattc aattcaaatt
    11401 ttgtaactaa ttccgctttt attgttccat ttgtttccat ttggcctaaa caaagtaaag
    11461 gcaaaaacag cattatatat actagctttt tcataaaatt agtttacgat tatatcaatc
    11521 ttatatttct cctctttagt tattgttttc cattcatctg aacgaaacgg aacggcagga
    11581 aatccttcta cgttaaacaa attacattca gaagcatcac ctatccatcc gaagcgaaca
    11641 gcgattggat ttaaaacatt ttcattactt actaccactt tattttcttt gatgtatgct
    11701 tttgccggat agaaaacttg attttcacct gcaatttcaa atccgttaac aaacccatat
    11761 tcattggtgg ttactaatcc tgctcctaaa gaattaaaag taacaattag ttgcttctct
    11821 ttcttttcaa aagagttaaa ggttggtccg ttagaaatca tgttttcatt atttaaatta
    11881 gtaaaagcta tagatgccaa tcgttttgca acagttttct tattcgttgg atggatgtca
    11941 tttgggtttc caacatcagt agttacaacc attcctgtgt tttttactga taatgttttt
    12001 gtttgagctt cgcgaatttc tgcccaagca catccttcat tactatttcc tgttgtttga
    12061 taagttgcta attgcacata gtaaaaaggg aaatcatttt tccatttttt acgccaatca
    12121 ctgattagca tcgggaatgt ttttcgatat tgaaaagctc ttgacacatt actttcacct
    12181 tggtaccaaa gtactccttt aaaagcaaaa ggaattaatg gatgaatcat agaattgaaa
    12241 caaagtgaag gaaaatcgtt ttcattaata ctatttttaa ttgattctac ttgaaatttc
    12301 caatacccgt ctaatggaat ttctttgttg ttcagtataa ttttcaatgc tgctgcattg
    12361 ccataaatgc ctccgccgcc gccattgtca accacgcgaa tagtgataac attatcgcct
    12421 aattttaata tccccgaagg aattttatac attcgctttt catcccaacc atttgtttgt
    12481 ccaattttta tcccatttat aaacgtgatg tcattatcat cgattgccgg aatttctata
    12541 atacaatttt cagtgatttc agaaagtgta aaatgctttc ttaaccaaat aacgccatca
    12601 aaatttccca attgttgttc ttcccaaagg ccgggttggt gtatttctga ccaattactg
    12661 tctttaaaat tcaaatcttt ataaaaagaa acatcctcag gatttaattt tgaattttga
    12721 attttttcaa ttcgagtttt tgcagcatcc attttggcgt ctaaaagtga atgaatatca
    12781 attttaggca tactataaat catatctcgg aactcactgt caagttcaaa accttctcta
    12841 ctaatccacg tttcaatatt tgttcccccc caagaagcat tgattattcc tattggaatt
    12901 ttcagtttat tgtttaattc ttttgcgtaa taataaccta ttgccgtaaa atccgctact
    12961 gtttttgggg aacaaacttc ccatgctgtt ttttgaatat ctgaagcggg aaaggaatta
    13021 atttcttttg gtattttaac atgtctaatt tgaggaaaat cagccatatc tatttctttt
    13081 tctgcttcat ccgcttgccc aaccgtccat tccatatttg attgtcctga acatatccaa
    13141 acttcaccca ctaaaatatt tttaatttca atcgaatttt tcccttttat tttaagaaca
    13201 taaggaccac cggccatttc atcatctaaa cgaacaatcc attttcctgt ttgatctgct
    13261 ttggttgttt tagtttgatt atgaaattgt acagtaactt tttcattggc attagcccag
    13321 ccccaaaccg gaattttaga gtggcgttgt aaaaccatat tgtcagaaaa aatagcagca
    13381 acttttactt gagcctctaa agcgaatgaa ctgataaaag ttattagtaa aagaaaatgc
    13441 catttaaatt tcatgttttt catattcgta tacttcttta gtaaaagcta tttccatttt
    13501 taatctttca aattcatttt cattatttgc taaaaagata gtagctactc cgttacctat
    13561 aaaattagta atagaacgtg cttcactcat aaatcggtct accccaagca gcaatgctaa
    13621 tccttcaata ggtatcactt taaccgcaga aagtgtggat gctaatacaa taaatccgct
    13681 tccggttact cctgctgctc cttttgaagt tatcattaat acgccgataa ttgttcccat
    13741 ttgagtccaa cttaaatcaa tattataaac ttgagctaag aacaaaatag ccatcgacaa
    13801 ataaatcgtt gttccatcaa gattaaacga ataaccggca ggaacaacaa gtccaacaac
    13861 tgattttgag caacccattt tttcgagttt ttccatcaag gatggcaatg cggattcaga
    13921 agatgaagtt cctaaaacta ttaataattc ttcttttata taattcaaaa agtttaaaat
    13981 gctaatttta tgatatttaa gaatagcccc aagtacaata aaaataaata acgccatggt
    14041 gacatatacc gttatcatta attttgctaa aggaatcatc gttgctattc caaatttacc
    14101 tatagtatag gcaattccac ccaaagcacc gagaggtgca aatagcataa ccaaatggag
    14161 tgctttaaaa acatattttg aagcaaagct gaggtatttt tttattctat cactgtgttt
    14221 gtatttattc aaaaatgtcc caaaaaggat agaaaataac aaaacctgaa tggttaggtt
    14281 tgattttata aagttccata atccaaatgt ctcagcaccg gttgtatatt ttgaaatttt
    14341 cgccggctgc aatgaaatcg tttcaattcc tattcccggc tgaataatcc aagttacaac
    14401 aacacctatt aaaagagcaa gtgtagtgac aatttcaaaa tataaaagtg ctttccctcc
    14461 aactcttccc acttttttta aacttcccat tccactaatt cctaaagaaa tggttaagaa
    14521 aattattgga ttgatgaaaa ccttaactat attgataaaa taagttccta aaggctgcaa
    14581 ttgaatagct gtttcaggca aaaaatgacc taatatagcc cctaaaagaa ttgctattaa
    14641 aacccaagta gttaaattgg tgaaaacctt tttcatttaa ttggtttaga aaaccttaaa
    14701 aaattatgaa accttaagag gtttctcttt acttattgat ataaaatatt tcgattctag
    14761 aaattaataa taaattcaaa gctacttaaa ttatttcacc aattcaaatc tgccttttaa
    14821 tctaatatct gtagaagacg acccaatcat tatgtcaaaa tcacctattt cagaatcgaa
    14881 taccagtttt tcattataaa aagataattt ttggttatca atactaaatt taatggtttt
    14941 tgtttcacct acttccaatt ttattttatt aaaatctttc aattctacta ttgggcgaac
    15001 aacagaacct actttatccc gaagatagag ctgcacaact tcttcaccta taaattttcc
    15061 ggtattagtt agtgtgaaag aaacttctat cgtttcattg cttttcattt tttgctttga
    15121 taatttcaaa tctgaataag caaatgacgt ataacttagt ccatatccaa aagggaattt
    15181 aggtgaattt ggtaaatcta tataagctga tttgtaaatt ttatcttcat ttacagatgg
    15241 tcttcctgta ttaaaatgat tataataaat tggaatttga ccttcttctc taggaaaagt
    15301 cattggtaat ttaccggacg gattataatc accaaaaagt acgtcagcaa ttgcatttcc
    15361 tgcttccgaa cctaaccacc aagtataaac aattgtgggc atattgtcgg ctatccaatt
    15421 aaaaattaat ggacgaccgg catttattaa aatcacgatt ggttttcctg ttttctgaag
    15481 ttcttttact agatcttcct gaacacccgg caaatgaata ttacttctgc ttttggcttc
    15541 accgctcata ttccaagctt ctccgatgct taaaataact acatctgctt gactggctat
    15601 gtcaactgct tcttgaaatc cttctttgct agtgttttca actccacaac ctttcgcata
    15661 aagtaatttc gtgttctttc ccactttgtt ttttaaacca tcccactgcg aaacaatgta
    15721 cgttgaatca atttctttta aatctaccgc ccaaaaaccg tgattgattc gtttaggttt
    15781 aaccattggg ccaataaatg caatgctttt tatactttta gaaagcggta aaacattgat
    15841 ttcattcttg agtaacacaa tacttttttt tgcaatatct ctagcaattt ttgtatgctc
    15901 cggattattt aacgcttgat tttgtctttc cagattgcaa aaacgatacg ggtcgtcaaa
    15961 taaacccaat tcaaattttt tgtgtagaat tctttttacg gcgtcatcaa ttaaagccaa
    16021 cgaaactttc ttttctttca ccaattgttc caaattaaac cgatatgcgt tgctttccat
    16081 atccatatca gaaccggcag ttatggcttg taaagcagct tctttagcat cttttgaata
    16141 accgtgagca atcatttcac caatagaacc ccaatcagaa actacaaatc ctttaaagcc
    16201 ccatttctct tttaaaatat cgcgttgtag atatttattt ccggtagcag gaattccatt
    16261 gatatcgttg aacgaattca taaacgttgc tgcacccgca tcaatagcag ctttaaatgg
    16321 tggcaaataa gtttcccaca acattctttc gctcatgtca actgaattgt aatcgcgacc
    16381 accaaccgca gcaccatagg ctgcaaaatg cttagcacaa gccatgacag catttaattc
    16441 gccaagtttt tccccttgaa atccttttac gcgggcaaag gctattttag aacctaaata
    16501 agtatcttct cccgcacctt ccataactct tccccaacga gggtcgcgtc caatgtcaac
    16561 catgggtgca aatgtccaat gaatacctga tgccgcagct tctgttgcag caattctggc
    16621 accaagttca atggctgata aatcccatga agcagcttca gccaaaggaa ttgggaaagt
    16681 agttttatac ccatgaataa catctaaacc aaataataat ggaattttta aacgtgattg
    16741 gatggcatat tcctggtatt gccttgtata agtagaaccc atcacattca gcattgaacc
    16801 aattttccct gattttatct cttctttttt gttaggatta attgttaacg gtccggtaac
    16861 tgtattatca cctgtatatt ggttaagttg accaattttt tcttctaaag tcatttgttt
    16921 caaaagatct tctacctttt gattaattga cttttgctgt gaaaatgcta caaatgattg
    16981 aaatcctata aataaaaata ttactttctt cttcatccaa attgaattaa taaaccaaag
    17041 tttggatagc ttttgcagga attttgattt cacttacttg attattgttg ctcaataaat
    17101 aagaaagttc tttgtctgat tcattcatta caatggtaac taattcaccg gttgggtttt
    17161 taaaagacgt tacaatcaat tgacttctgc tacttgtaca actaaagttt ttagctccgg
    17221 atttaatgaa ttttgaaaaa tgtccaataa agtaataaga aggcgtataa attaattctc
    17281 cagtagaagt atcgccatga attggtgaaa aacaaaaatt acctacatga tttggccctc
    17341 cattttggtc tagaagtata ttccaatccg tccaacctac ggttccgtta ttaaaatcgt
    17401 taatcatgga acgtccgtaa cgttgaccat ttgcccaaaa ctggtatttt tcagcactaa
    17461 atttttccac acaaccttct gtaaataata aatttttatc cggatacatt tgatgcactt
    17521 tcgccacgtt ttcaaacatt ggatttcccc cactccagtt ttcataccaa tgaaatccga
    17581 ttccccaaac gtattttgca gcttccgggt catctaaaac agcacttgct ctttgtgaaa
    17641 gtaaatctct gttgtggtcc caaactgtaa ttttaacatc gtttaaacca tttttttgta
    17701 atgtaggtcc gaggtaattt tttaaaaatt ctctttcttc atcgccggta aaaatacaag
    17761 attcccaaat ttgagtagcc atcggttcat tttgaatcgt tagtccccaa accggaatgt
    17821 tctccttttg ataagcttga acaaacttta cataataatt tgcccatgat tgataaaatt
    17881 caggtaacaa tttgccgcct ttaagcatgt ttttagaatc tttcataaat gccggtggac
    17941 tccatggact agcataaaac gttgctttgc cggctttttc cattgctttt ttgattaatg
    18001 gaattttaaa agctcggtcg tgttcgatag aaaaagtttt caattctttg tcgccttctt
    18061 caacataagt ataactttct gaactaaaat cacaactgtg gatattggtt cgaataagag
    18121 tataaccaat tcctttttct ttatcaaaat aggcatccaa aaattcattc tgtttagtaa
    18181 gaggtaattt agcaaaaacc tcggcactgg catcggtgat tgctccccca attcctaaaa
    18241 aagtttgatt tgttttattg gggtgaacaa aaacgcacaa ttgggtttcc aatggttgct
    18301 tgtattcttt aaaagtgagc acatcggttt tagctagttt tagattagaa tcaggtgccg
    18361 tagtataaac cgtaacagat tgatgatttg gtaggtttga atttatttta tctttagttt
    18421 gtccaaatag tacggaagaa accataagtg tggagagtaa tattttcatg tttttttgtt
    18481 tttaatttac catgtatagg ttccaacagc accggcatct agcgaagtgg taatccattt
    18541 tccgttatat tttatattga ataattcaga attatttcca tcattgacta ctataactac
    18601 tttttttcca tccggtcttt taaaagctac gttgtataag tttccaacca ctgaacttcc
    18661 aattcgaata gaaccttccg gaacaaattt agaagcatgt ccgataatat aaaaaccaac
    18721 attcttagca aaggacgaac tactgttgat tgtgattgcc cctttacaag ttgtacaacc
    18781 tccgggagta tgcggcccaa aagaggcgtt gttcgctaaa ttccattcta aggctatttt
    18841 actccaattt cgcatggagc caatgactac atttttcacg tgccactgca aatcaccatc
    18901 aaaatttccc gtattggaag tatactgttc agtaaaatat aaatctttgg taggatgagc
    18961 atttttaaca atagaaagtg cactaatatc tcctccatat aagtgaaaag cagaaccggc
    19021 aatgtatgat tttgcagagg catcgtttaa aacttgaatt ggatattccg gtttatcaca
    19081 attatgatca taaataataa tttttgtagt tattgatgct gtttgaaaag ctggtcctaa
    19141 atgatttttt ataaaatctg cttgctgagt agctgtcata tacatactgg gattatttcc
    19201 cggatgtaga ggttcatttt gtggagtaat agcatcaata ataattcctt cagcttgcat
    19261 cgcttgaatg tattttacaa aatagttagc ataaacgctg taatattgtg gttgtaaact
    19321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cccatcatta ccaatagaaa
    19441 taaaaatttc attttatata gtttgttcat tatttataat tcacttcgat gaatacaggc
    19501 aaatgatcgg aaggatattt taaatctttt gaatcactta aaactgcata tttgttaatt
    19561 tttagggtat tgtttttaga cacaaaaatg taatctatta aaattgtaac cggttcattg
    19621 tgtttaaatc cgttaaaagt tccggtaggt ccaaacggtt tttgaatggc aatttcacga
    19681 ctgtcattca tttttgattg tagtgaaata attctttccg tagaaggttc cgaattgaaa
    19741 tcacccataa aaaaaacagg ataatttttt gtatttactt tttctatttg ttttaaaatt
    19801 aattcaattc ctttggttct cgcaatctca cccacatgat ctaaatgtgt gttgaacacc
    19861 caaaaaagaa tgtttttttt agtgtctttt aataaaacat aggtgcaaac tcgataaaaa
    19921 gcagcatccc aaccttttga aatttgattt ggtgtttctg acaaccaaaa cgtattttct
    19981 gttattactt taaatcgctc ttttttaaaa taaatgtttg aagattctcc ttttccctct
    20041 ccttctcgcc caattccaac ctgattatat tgagttaact cgtttgaaat atcaattact
    20101 tgattaggag ttgcttcctg tattccaaaa atatcgggtt cataaaattt gatttgggct
    20161 gtaaaaaaat cttttctgtt tgcccaacta ttttcaccgt ctgaagcaac atccaaacga
    20221 atgttatagg tcatcaattt caaatcttgc gatattccta taagtggaat aagaaaacaa
    20281 caaagtaaaa ttgatttttt catgatatta ttttattgta atttcaagga ttccaaagaa
    20341 ataaaagtct gcttttttgt tctttacaat gcttctattc aacaagaata gaagcaaggc
    20401 aaagaaacaa ctaacttaaa caaacatctt attattggta aactctaatg taatcaactt
    20461 ctaaagttgt tgatgtaaaa tttggatcaa tattccctcc aaaatttcca cccatggcac
    20521 aattaaaaat taaaaagaaa ttttgattaa atggcaagct attagtattt acaaaagaat
    20581 aatacaattg attatccaca taaaatttga tgtacgatga tgtccaatct actgcataaa
    20641 cgtgaaattc tgaagaggca tttgaaatag ttacatttcc accgtccgca tttcctcctg
    20701 aatgacccgg atgatgaagt gttccataaa ttttattttg agcatttcca acatgttcca
    20761 tgatatcaat ttctccacat cctggccaac cggcagtccc tatattgtcg ccaagcatcc
    20821 aaagtgccgg ccaagttcct cctccggtag ctaatttggc tttgatttct actcgaccat
    20881 atttaaaaga aaatttatct ttcgttacta ttctggctga agtataattg ctaccactgt
    20941 aaacttcctt gattaaattt atttttaaaa taccgtcttg cacaattaca ttttcaggac
    21001 gattagtata atattgagct tcattatttc cccatccatt tgaaccggtt ccaatgtcat
    21061 atccccaata ggttgaatta ggagctccat taacattaaa ttcatcagac cataataatt
    21121 ccggcgaaac ataaaccgtt gttgcaatgg ttttagatat aaacttagtt ccattatagg
    21181 cagaaacata aattgtatag gtatttgtgc ccatatcggt gaaagtatga tttactaatc
    21241 cggttgtaga ttccaccaac tgcccgtttc caaaattaat tttatacaat gttgcgttaa
    21301 ccgcagttgc agtcaatgaa atcgtaccgc ttccatcacc atttggagta gaatctgaaa
    21361 aacctacttt tgttatggta actgctaaat ttgatggcgt gatagtatca cctccatctg
    21421 aactatcgtt gctacaacct aagataggta agcaaaacag tgtcataaaa aggtaaagat
    21481 tctttttgaa agcggtgcag attttcattt ttttttgttt taaaaactac cccgaaaaaa
    21541 tattcctttc ggggtaattc aatttagttt taaggagctg gttttaattt taaataccag
    21601 gcatttccgg tttccgtacc ttgaactctt aagtacatat aatttccatc taaaaccata
    21661 atttcatatt ctttttgcaa tgacccataa cctatgaaag ttgtggttcc tgacaacaag
    21721 atactagttt gagtagaagg cgttgaagcg tcaattccgg tactagaagg cacaaaactg
    21781 aatgaacttg aaccaccggc ataaggataa caaccttcat ctcctgaagc aggaatacca
    21841 gggattgagg ctaacgaacc tgtttttgtg aaagctccat cagaagtaac tacgtcaagc
    21901 gtataagtat tagaaactgt tttagaaaat gtaaaagtgg ctgtgtaaaa acaattacaa
    21961 caagcaactt tttcatcaat tgctgcagac caccattctg gagttgatga atttgcccat
    22021 ggtccaacac caaagtgacc aggaacactt ttatcaacaa tccatttttt tgaagaatta
    22081 ttcgtcaaat tagttacaat ttcaggattt acattaaaat cactgcgaac cgtaatatct
    22141 tttgtaataa tcgtagaaga accacctttc ccataagcaa ctgctgttat tctataggtg
    22201 tttacaccta atgatgtgta ttttttagtt actttcccag ttggaagata tactaaatca
    22261 acatcattat ttgcatcata atcaacttta taagacaatg cattatcggc ggtaatagtt
    22321 atttttacat ctcctgaacc atctccttca ggattattaa tatcttgacc aactatttct
    22381 gtagcaatta ctaaattagt tggtgcatct aaatcaccca aagaataatc ttcatcatta
    22441 ttacaggcaa aaaaaatggc acccataaaa acaagagcaa atatatattt aaaatttttc
    22501 ttcatttttt cttggtatta attagtttgt ttaatgttat caaggtaaat tacttccccg
    22561 gtaccgttag ctgtatcgtt atatctgaat actaattgat taaaagtagt tccggcagga
    22621 attccaaatg tcccaaaatc aaaagatagt tcttcccatt gatttgctac agtagaggct
    22681 accttcagaa ttttatttga aaccccatct gagccttgtt caagttctac attaagcatt
    22741 ttacccactt ctgtagaata aaccataacc ttgattatac ttccgttatc aatattgatt
    22801 ggtgttccgt ttggagtact gagcggtgtc catgttcctc cccaaccagg agcaccaact
    22861 gcttttatat actttcttac ggttgcacta gtattgattc ctcccggatt tggattagct
    22921 atttgcgaaa cacttacatc tccaaaagtt ccaccaatat tataattttg gattggactt
    22981 tcataggtaa ttggtaatga aaatggagca taaattgtaa ttgattctgt taattcagat
    23041 tgagctgctc caccacttaa agcaatcact ttaacattat atactccagg tgctgcataa
    23101 gtatgaggag gtaatgtttc tcctactgcc attggtgtac caacttcatt tgtaacatca
    23161 ccaaaataaa ctaaaaagcc attcgcgtaa tctgctgtag cagaaactgt taaatcataa
    23221 ccggctaagt ttttattgat tactaaattt tcaggagctc tgtaaaccaa ttgcaacgga
    23281 taagtatttt ctacttcaat tccggtgatt cctcttgaca ctatggttac ggtataactt
    23341 ccttcagggt aagaatgtgt tacactattt ccggggctaa gagaaactaa ttctccatca
    23401 ccatgaccta aattaaccgt aaattctgtt actccttcac caaaaggtgt tattcttaca
    23461 tttcccgaat tatcatcgct gatatcgaat atttttgaag ttgcagttgg ttcagatgaa
    23521 tttagggaag acaaatcagt cccaattcca tcttcttcag agcaacctat tactactgtt
    23581 tgtaataaaa caaaacagat tagaaattta atttttttca tattactata cgtttttagt
    23641 aaacccccca tgtcatcaat tatttttgga aataagataa catgggtgat ttatttttct
    23701 attagttata acttggattt tgaaccaata aagtgttttc taactcttta aatggaattg
    23761 gcaatatttc gtgttttcct gccacaaaac ctcttgaggc tagttttgaa gcagcttgac
    23821 ctgttcttac taagtcaaac caacgatgac cttctccggc taattctctt cttctttcat
    23881 ccataattgt agtcattgaa acagggacag atggtaaacc aacgcggctt ctcaccgcat
    23941 ctaataaggc ttgtgctctg gttccgcttc cgcctagggc ttccgcctcc attaaataag
    24001 tatcagcaag tctaattgca taggtgtttt gtttataatt caaaactgca tctccggctc
    24061 cggtatgtgc atcagcttgt ctaggtaaaa atttatttaa aaaatagcct gtgtcttcat
    24121 aaccaccaat gtagtcaact tcacctgcta atttcaaggc tttcaaatct aacacagtcg
    24181 ctccaaatct tggatcatca gccataaaat cataaaaatc ttgtgtaaac acattaaaac
    24241 tccaaccgga tggcaaggca ggtgctgttg aaccaatagc cgtataacct ctaggcccaa
    24301 ccattacgtt aacagtattt ccttcatctt gaccaccgcc ccaaattccc caatacgaat
    24361 ttccttgact agtatgagta acttctaaaa tagattcggt gttaaattta ttggtaacca
    24421 cccataaatc attaaaatta tctagcaact tatagccata ttgactagta cctcctgggg
    24481 tgccattcac ttcttgtaat tgttctgcag ctaatgcatt tttaccacct tggtataagt
    24541 aaactttccc taataaggct tgagcagaac ctttagtaaa acgaccagct tctgtagata
    24601 aacttactgt tgcaggtaaa tcagcaatgg catcaattaa atcctgttct atttgtgcat
    24661 acacatctgc aggatttgcc tgtggaatgg aatagatttc agtgggtgat aatggattta
    24721 aaattaaggg aatgttttta aacattctta ctaagttaaa ataataatac gcacgaagag
    24781 ctttagattc agctgtaaat cttactttta aatcatctcc catcggaata ttaggtattt
    24841 tacttaataa cacatttgct ctaaaaatac cttggtaata atcattccaa aaactagcag
    24901 gaatggtatt agaatcaagg gtgtaatttg agaaagattg aataccaaca ccatctgtag
    24961 caccaccacc tccggcaacg tgatcgtctg aaccggcatt aaacatggta atcatgtttt
    25021 cgaaaccgcc ggaatttttt ctcatcacat catatacagc tacaagtcct gcataggcct
    25081 cgtctgcatt ttgataataa tttgactcta aagccaaacc tttaggctcc acttctaaaa
    25141 aatcggagga gcaagaaact gccaacccta tagaaaaggc agaaacgatt actaatttaa
    25201 atttatgttt catagttttt tatttttaga attgtaagtt agccccaaac ataattgaac
    25261 gagcttgtgg ataatatcct ctgtcaacac caaatacact acccccaatt tcaggatcat
    25321 aaccagtata ttttgtgaag gttaacaagt tttccccagt aacatagaaa cgtaattttt
    25381 gtaatcctac ttttcctatt acatcattag gtaatgaata gccaaattga attaatttca
    25441 aacgtaaata atctccatct tctaaataaa aatcagaagg cttaccaaag tttccattcg
    25501 tatcattgtt tgttaatctt gggtaactgt ttgaagttcc ttcaccggtc catctgccta
    25561 aggcatctgt ttgataattg gcattaccaa catccaaacg tctgagtcct tgaaagattt
    25621 tattccctcc tgctccttga gcaaatgcca taaaatcaaa tcctttgtaa tctaagttta
    25681 tggtaaatcc ataattatac gttggcaatg aactccctaa gaaaactcta tcttcatcat
    25741 taattgttcc atctccatta gaatcagtcc atctgaaatc gcctggtctt gcatccggtt
    25801 gaattaaacc gccatcgcca ttagtgtaag cattaatctc atcttgattt tgaaaaattc
    25861 cggctgtttg ataaccataa aaagaattat atgcttctcc cacttgcgtt ctggtaatag
    25921 gtcccattga ttggaagcct gcagaaccat tggtaatata ctcaattcca tttcctaaat
    25981 gagtaacttc attattcaac gtagagtagt ttgcatttac agaaaagtta aagtctccaa
    26041 atttcttttt ataccccaat tcaaattcta accctgagtt ttccatgtca gcaatattcg
    26101 ctaaaggctg acctgttgca cctacatagc caggaatttg ttgatattgt aaaattccag
    26161 tggtgacttt tttataccac tcaaacgtaa aattaaaatc attgtatatt ttagcatcaa
    26221 aaccaatgtt ggtttgagcc gtttcttccc attttaaatc agggtttgaa ggagcatttg
    26281 gactatatcc tgaagtaata tttccggcat ctcctatagt gtagtttctt ccaccaccaa
    26341 tggttgatag ataaccaaaa tcaggaattg catcattacc ggtaacacca taaccggctc
    26401 tgatttttaa attagtaacc actttatttt ctttccaaaa atcttcatta gaggcaaccc
    26461 atcctacgga aaatgaagga aaaacaccat attgattatt ttgaccaaaa cgtgaagaac
    26521 catctcttct gattactcca ttgaataaat atttttcatt ataatcataa ttaacacgag
    26581 caaataagga atttacatta tgttctactc catcaacaga ataggaagaa cgttgagatg
    26641 caggaacatc aaaattaaaa gaagcatctt ggtaattagt taccggaata tcaaaataag
    26701 ttacacctgt ttcagtatat attttattgt cctgataagc accttgacct aataataccg
    26761 taatattatg tttttcaatt tgtttggtat aggtcaacgt attttcaata ttccaaccaa
    26821 aactcttatt gtttcttctg gaaatattat tttgtgaggt caaagtagaa gcatttaaat
    26881 aatattcagg cgtgaaacct tctgctcccc aataagctaa tttaccacca agtgttgttc
    26941 taaatttaaa atccttatgt aatttaaagt caaaataaat atttccaaca aaatcatcag
    27001 accaaccata ttgtcctaat cttgtttgaa tataagctaa aggattagtc atctcttgac
    27061 ctactaaaga agaaatacca taaggatttc cgttggcatc tctaataaca ggattgttat
    27121 tataaggagc cgcattagca atagcaggat ccgttactac aacaggtgta gttgggtcta
    27181 agttaatcgc tgaacttaac ggccctccat attcactgtt tgtatttcca agtccaatcc
    27241 ctttttgatg agtataacca agtgtttgac caatagtaac ccattcttta aatttatgtg
    27301 tggaatttat acggaaattc tttttattga aattagaaat ttcggtagct acaattcctt
    27361 cttgatcgga agaaccgaat gataaaaaga atgttgaaat ttcacctcca ccacctacac
    27421 ttacttcatg tgaatttcgt tgagcattat tattgaatat ttgtttttgc caatctgtac
    27481 caactcctaa agttgataaa tctggaaaaa ctaaattttg tcctgctgct accgcttttt
    27541 cattcatgat ggcaccatat tccgtagcat ttaataaatt aagcatttta gctggagagg
    27601 aaacaccata aaatccatta tagtttaaag tcattttacc ttgttttccc tttttagtag
    27661 tcactaaaat aactccggat gcagctcttg ctccataaat tgcgagcgat gcggcatctt
    27721 ttaaaacttc catagattcg atatcagctt gattaacaaa acc
//
LOCUS       DAOTCW010000012        27077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_888_length_27077_cov_43.715861, whole genome shotgun sequence.
ACCESSION   DAOTCW010000012 DAOTCW010000000
VERSION     DAOTCW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 27077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27077
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_888_length_27077_cov_43.715861"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="P5335_01885"
     CDS             <1..837
                     /locus_tag="P5335_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRZ73660.1"
                     /translation="TGSSLKFMMDAFPERAFDVGIAEQHAVTLSAGMATQGMVVFCNI
                     YSTFLQRAYDQVIHDVALQNLPVIFCLDRAGLVGEDGATHHGVFDLAYLRCIPNMIVF
                     SPRNEIELRNMLYTAQLGLNHPIAIRYPRGRGEIVDWQIPFQKTEIGKASLLKKGSKV
                     AILSNGFIGKNVTTALTLISAQNDFSHYDFPFVKPLDEQLLTSIFKDHESIITLEDGT
                     IIGGFGSGILEFKAKNKYKNEVQVLGIPDEFIEQGTIDELQRFSKINVENLVSILSTY
                     SK"
     gene            872..1822
                     /locus_tag="P5335_01890"
     CDS             872..1822
                     /locus_tag="P5335_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HRZ73661.1"
                     /translation="MKSISSTLFLLLSSSVLFSQPLQTVKDSTLKDTTYWVQKKIVGL
                     DITQIAFLNWNAGGNSSISGLFKGDFSRKYTKENVLWNNEMSIRYGINKQDDRELRKT
                     DDAFSILSNYGYKTDINSNWYSSARFNFNTQFTNGYAYPNIDTPISKFFAPAYVFLGI
                     GSEYNRKDWNANFYFSPLTLKTTFVLNQRLADLGSYGVTKAIYDEEGNLLKRGRKSRT
                     ELGMLFNNYWKTEIYKNMTLENRLSLYSDYLNNFGNIDVDWQLQVDMIVNEYVKANIG
                     IHMIYDDDIKAKEEVNGEQITVGPKLQLKQALGIGVVYVF"
     gene            complement(1873..4485)
                     /locus_tag="P5335_01895"
     CDS             complement(1873..4485)
                     /locus_tag="P5335_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS-dependent M36 family metallopeptidase"
                     /protein_id="HRZ73662.1"
                     /translation="MKKITFLFFVLFAFSVNAQTETQKIQEYLNSNYSTLGITSQDIN
                     DWYVESEATSSSTGITNYYVKQRYQGIEIFHAQTNFSIKNGNVVYVANRFESNIAQRV
                     NTTTPAYSILDALSLVYGSFNITPIESFQIQRTIRTNYYQINDAIGINEPVLAKLVYQ
                     LNEENKLRLAWDFTFYSPNHKNLWSVRIDAKNGEILEKQDMVVSCSFGKDSDHSKHQH
                     YVPFTKQLFKEESAISVVETQSGSYRVIPYNIESPNHGERQLISTPHNATASPYGWHD
                     TNGVDGAEFTITRGNNTWAKEDRNGTNSVFGAAPNGGAILEFDFPYGGNSAQSTTYTN
                     AATTNLFYMTNVMHDVWYQYGFDEANGNYQANNYGKGGTAGDFVYADSQDGVGTNNAN
                     FSAPVDGQSGRVQMFLWDVGPPNYSLTVNTPATIAGPYAIRDNNFIPGNVPLPIAPNG
                     FTTDLVLFDDGSPDNSDACSPAINAAAISGKVCILRRGDCTFVEKVKNAQNAGALAVI
                     VVNNVEGVIIMGGADATITIPAISINQDVGEAIIAQMSLGVVNVTLQTPADVFINADG
                     SFDNGIISHEFGHGISIRLAGGRNNSSCLQNAEQMGEGWSDWFALMMQLKPGDVGTAI
                     RGIGTFAVSQPIDGVGIRTYPYSTDMSINPMTFNSTNTEAVPHGVGSVWATTLWDLTW
                     AYINKYGYDSNIYTGTGGNNKIMRLVLDGLKLQPCSPTFIEARDALIAADQATTGGQD
                     YCMIWEVFANRGLGLNASSGNRNSSTDQVEDFTMPPAGPNCVLSVDYFATNEVFKVYP
                     NPSNGMFTIQVNQFSGKVAIQIVDLNGRIVYQENEVNFNNAKNINLTHLQSGMYIVKV
                     NADELNFSQKIIKN"
     gene            complement(4561..5904)
                     /locus_tag="P5335_01900"
     CDS             complement(4561..5904)
                     /locus_tag="P5335_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRZ73663.1"
                     /translation="MHWEQLLSLKRQGDTGKRLRSEQDDTRLGFEVDYDRIIFSAAFR
                     SLQDKTQVIPLSKTDFVHTRLTHSLEVSVVGRSIGRLVGKKIIEKYPYLKEIHGFHMN
                     DFGAIVAAACLAHDIGNPPFGHSGEKAIGEYFKTGNGQQYKNQLAEKEWQDLIDFEGN
                     ANGFSVLTGNRPGNEGGLRISYATLGAFMKYPKESLPKKPTTAIADKKYGFFQQDKAF
                     FKEVAEELGLISNKTSDNIGYERHPLAYLVEAADDICYTIIDFEDGINLGLVNEDFAL
                     EYLIKLVKDSIDVSKYNSLQTKEDRISYLRALAIGSLINDAVRIFIDNEEHILKGQFP
                     FALTDKSKYKAQMDDIIKLSVKKIYQSREVIEKEITGYHVINNLLDKFITAFNNQYHG
                     KMSNYDVLLLKVLPEKYQVNKETLYERLLHISHYISLLTDGNAILLNKIVSSNNS"
     gene            complement(5909..6415)
                     /locus_tag="P5335_01905"
     CDS             complement(5909..6415)
                     /locus_tag="P5335_01905"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRZ73664.1"
                     /translation="MEFVKPKEIAITSAMLASSGQRLGNYLIDMVIHNIINVIPIGIG
                     WILYEFFGNYELSLWLESINPIVDFLISYLIIVIYYMIFESLTGKSIGKYVTNTKVMM
                     ADGSKPEPYSIFIRSLSRLIPFDQLSFLGTIPRGWHDTLSKTVVVDLKKYNQELEMQD
                     SINEIGQE"
     gene            complement(6431..6658)
                     /locus_tag="P5335_01910"
     CDS             complement(6431..6658)
                     /locus_tag="P5335_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73665.1"
                     /translation="MSKDKKGVYTGIIEKDEKGNYFCGEYLLDFKYTEANFKLGDLIN
                     IKTVIENPSDISYNQYPKKSRNFFLANDKKA"
     gene            complement(6756..9170)
                     /locus_tag="P5335_01915"
     CDS             complement(6756..9170)
                     /locus_tag="P5335_01915"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HRZ73666.1"
                     /translation="MYVVKRDGHKEPVMFDKITDRIKKLCYGLNDLVDSVKVAMRVIE
                     GLYDGVTTSELDNLAAETAASMTIAHPDYAQLAARIAVSNLHKNTKKSFSETMSDMYH
                     YVNPRTGQQSPLLSEEVYDAIMANAEELDSMIIYNRDFNYDYFGFKTLERSYLLKING
                     KIVERPQHMLMRVSVGIHLNDIEAVKETYELMSKKFFTHATPTLFNAGTPKPQMSSCF
                     LLTMKDDSIDGIYDTLKQTAKISQSAGGIGLSIHNVRATGSYIRGTNGTSNGIVPMLR
                     VFNDTARYVDQGGGKRKGSFAIYLETWHADIFEFLDLKKNTGKEEMRARDLFFAMWTS
                     DLFMKRVQDDASWTLMCPNECPGLCDVYGDEFEALYHKYEAAGKGRKTVRARELWEKI
                     LESQIETGTPYMLYKDAANRKSNQKNLGTIRSSNLCTEILEYTSPDEIAVCNLASISL
                     PMFIENGKFDHQKLYDVTKRVTRNLNRVIDRNYYPVPEAENSNIRHRPIGLGVQGLAD
                     AFILLRLPFTSDEAKQMNQDIFETLYFAAVTASMEMAKEEGPYSTFKGSPISQGEFQY
                     NLWGLKDSDLSGRWDWDSLRKEVVEHGVRNSLLVAPMPTASTSQILGNNEAFEPYTSN
                     IYTRRVLSGEFIVVNKHLLEDLVNLGLWNDTLKQEIMRHNGSVQNIEIIPQDIKDLYK
                     TVWEMSMKDIIDMSRQRGYFIDQSQSLNLFMEGATYAKLTSMHFYAWQSGLKTGMYYL
                     RTKSAVDAIKFTLNNDKKAEPTPEPQVVEVFAPNETGEMTAEDFKAMVEKARNAGPDD
                     CEMCGS"
     gene            complement(9290..10267)
                     /locus_tag="P5335_01920"
     CDS             complement(9290..10267)
                     /locus_tag="P5335_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRZ73667.1"
                     /translation="MAAVEPILQENKDRFVIFPIKHQDIWEWYKKQEACIWTAEEIDL
                     HTDLNDWNNKLSADEKYFIKHILAFFAASDGIVNENLAENFVSEVQYPEAKFFYGFQL
                     MMENIHSETYSLLIDTYVKDEAEKDMLFHAIETFPAIKEKADWALKWIESDSFAERLI
                     AFAAVEGIFFSGSFCSIFWLKKRGLMPGLTFSNELISRDEGMHCDFAVHLHTHHIVNK
                     VSKARITEILTDALEIERKFITESLPVSLIGMNATLMTQYLEFVTDRLLVELGCKRVY
                     NSTNPFDFMDMISLQGKTNFFEKRVAEYQKAGVMNSDSDSQKISFDADF"
     gene            complement(10525..11097)
                     /locus_tag="P5335_01925"
     CDS             complement(10525..11097)
                     /locus_tag="P5335_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HRZ73668.1"
                     /translation="MFQLGKTIVSEEILEKNFVCNLTACLGACCVDGDAGAPLSAEET
                     KILEDIYPKVKPFLRKEGVAAIEAQGTWVKGEDGEFETPLINGQDCAYVIFDGKTALC
                     GIEQAYNQGIVNWKKPVSCHLYPIRIKEFSDFSAVNYHRWDICSPACSLGKELEVPVY
                     KFVKEALIRKFGEEWFTELEKVGEEYLKGK"
     gene            11222..11797
                     /locus_tag="P5335_01930"
     CDS             11222..11797
                     /locus_tag="P5335_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRZ73669.1"
                     /translation="MELDLREIFTATMVLFAVIDIVGSIPIIIDLRNKVGHIHSEKAS
                     IVACIMMIAFLFVGETILSLIGIDINSFAVAGSFVLFFLALEMILGIKLYRDEQNEST
                     SIVPIAFPLIAGAGTMTTILSIRAEYQTINIIIAIILNIILVYGVLKSSAKIERLLTK
                     NGLSVIRKVFGVVLLAIAVKLFAANVKGLFE"
     gene            11836..12039
                     /locus_tag="P5335_01935"
     CDS             11836..12039
                     /locus_tag="P5335_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73670.1"
                     /translation="MKLFTSILLVLAFGLIIFNLTQLDYGNLFAQESMIALIGIVAAF
                     CAILILLIFRMSKTIEDKLKDQE"
     gene            12040..12651
                     /locus_tag="P5335_01940"
     CDS             12040..12651
                     /locus_tag="P5335_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRZ73671.1"
                     /translation="MFDLLVIGGGVSGVSCSLLLGSAQNKPFASDKKIGLIAHQKASS
                     LQEALFNNAYGISPGKLGSDLLSESLAHLQSTYPHIQQIPNEKVVKIDGDAGNYSVTT
                     NKNTYQTKLIVIGIGAGNPFTIEGLEEFVIPHQKINPEKNRIQLKNNDHLVKDGIYVC
                     GTLAGWRSQLPIAAGSGAAVATDILTLWNDGNHVQVHDSTKVK"
     gene            complement(12674..14245)
                     /locus_tag="P5335_01945"
     CDS             complement(12674..14245)
                     /locus_tag="P5335_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRZ73672.1"
                     /translation="MKIKKIYWPIVLFSAIALGVVIGGKLNYFSNKTTFNSGSYKSKL
                     NKLIDFIERDYVDQVDTDSIVDLTVNGILEKLDPHSVYITKNELNQVSESMRGDFVGI
                     GVNFYMYKDTITVIKPLNGGPSEKAGIKSGDRILFADNYKLFGKKLPNDSLYSKLKGE
                     IGSEVTLTVYRKSENKKLTVKLKRDVIPIKSVDVSLMINPTTGYIKINRFAESTFQEY
                     RKGLLKLKKQGAKTIVLDVRDNGGGYLDQAVQIADDFLVEDAPIVSTKNKKGRVDKTK
                     ATNNGLFENGKVIILINENSASASEILAGAIQDNDRGLIVGRRSFGKGLVQREMPLGD
                     GSAVRLTVARYYTPSGRSIQKPYVEGNEDYFNEFEKRFYNGELYEKDSIKVADSLKYK
                     TLKGRTVYGGGGIIPDIFVPLQGKHGDEALEMLMQSGVVSYFVFEQIDKNRTEFEGIS
                     FETFYKKAETTDLYVQRFQDYLKSSGVTFSLSSHKAMVKHYIIAEFSRQLFDEEKYFE
                     IRLKKDSMILEVLKQ"
     gene            complement(14276..14704)
                     /locus_tag="P5335_01950"
     CDS             complement(14276..14704)
                     /locus_tag="P5335_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HRZ73673.1"
                     /translation="MNKKKLNKYDKAYLRIAKEWSRLSYCKRKQVGAIIVRDRMIISD
                     GYNGTPSGFENCCEEESGTTKWYVLHAEANAILKVAGSTQSCEGATLYITLSPCKECS
                     KLIHQSGIKRVVYHEGYKDDSGLQFLLKAGVEVELITDLE"
     gene            complement(14729..15322)
                     /locus_tag="P5335_01955"
     CDS             complement(14729..15322)
                     /locus_tag="P5335_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HRZ73674.1"
                     /translation="MSEFWLYFEIGLKHVLDWNGYDHVLFLIALTVPYAFKDWKRVLI
                     LVSLFTLGHTLALLLSVYGVVVIKANLVEFLIPITILIVALFDLFTAGKSGKKESITF
                     VAFITIFFGIIHGLGFSNYFKTILPGNSSEKLLPLLEFALGIEAAQLIVVFIVLLLSY
                     VTQTAFRFSKRDWTLVMSSFVIGVVLPMIINSEIWNR"
     gene            15497..15928
                     /locus_tag="P5335_01960"
     CDS             15497..15928
                     /locus_tag="P5335_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRZ73675.1"
                     /translation="MSFSDLFDSGFKERNKGYFSSFVRIALSDNVLTDDEKKFLDRIA
                     LKLDISPEEYQEILENPAKYPVDPPYLYIQRLERLYDLGRMVYADHVLGPRQKDILTR
                     YALYLGFTPVNVTYIVDKALALLVHTVDLDTFIFEMQHMNK"
     gene            complement(15971..16978)
                     /gene="fbp"
                     /locus_tag="P5335_01965"
     CDS             complement(15971..16978)
                     /gene="fbp"
                     /locus_tag="P5335_01965"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRZ73676.1"
                     /translation="MEKRNTTLGEFIIEKQEEFQYSTGELSRIINSIRLAAKVVNYKV
                     NKAGLVDIVGAAGEQNIQGEDQQKLDVYANEVFIQTLINREIVCGIASEENDDFITVA
                     GSDNSHNNKYVVLMDPLDGSSNIDVNVSVGTIFSVFRRITPIGTPVTLKDFLQPGINQ
                     VAAGYVIYGTSTMLVYTTGHGVNGFTLNPAIGTFYLSHPNMKYAETGTIYSINEGNYV
                     HFPQGVKDYIKYCQLEEEDRPYTSRYIGSLVSDFHRNMIKGGIYIYPTSSKAPNGKLR
                     LLYECNPMAFLAEQSGGRASDGYSRIMEIQPSELHQRVPFFCGSLKMVEKAEEFMEKY
                     K"
     gene            17092..17565
                     /locus_tag="P5335_01970"
     CDS             17092..17565
                     /locus_tag="P5335_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRZ73677.1"
                     /translation="MIIRKGTPNDMPSVLELIKELAIFEKEPDAVVVTVNDLVRDGFG
                     EKPLFYSYVAEVKDEIIGMALYYYRYSTWKGKTIHLEDLIVKENHRGSRAGLALYTAI
                     IEQGKKDCVRRIEWNVLDWNLPAIQFYEKSGAKVLTDWRVAHMDEEGIKRFLNDK"
     gene            17605..18858
                     /locus_tag="P5335_01975"
     CDS             17605..18858
                     /locus_tag="P5335_01975"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRZ73678.1"
                     /translation="MKIFKFGGASVKDADGVKNVLSVLEKVGYEDVLLVISAMGKTTN
                     ALEVVIKNYFDKSEALNASVQEVRKYHNQILLELFEDDKHPVFKAINSHFEDLDYFLS
                     HNKSPNYNFVYDQVVSYGELVSTTIVSHFFNYSGLKNNWIDVRQFIKTDATYRDALVD
                     WEQTQKLISKGVKKKALNITQGFLGSDENNFTTTLGREGSDYTAAIFAYCLNAESVTI
                     WKDVPGVLNADPRYFENAILLNQISYREAIELAFYGATVIHPKTLQPLQRKEIPLFVK
                     SFVNPLLPGTKVSKGADLEPHTSCFIVKKNQLLISLSSIDFSFIMEENISEIFALFHH
                     YKIKVSLIQNTAISFSVCIEDKFGHFPELKTILSKKFKVSYNENVSLYTIRHFTEKAA
                     QMVEQNKTVLVKQISRETMQVVTKE"
     assembly_gap    18881..18980
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19082..19291
                     /locus_tag="P5335_01980"
     CDS             19082..19291
                     /locus_tag="P5335_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HRZ73679.1"
                     /translation="MKIKDISKFSTAEKIVLAEQLWDSISKKDISITEEIKKELDHRL
                     NLVEEGKTEIYSWEEIKANLEKSRK"
     gene            19288..19584
                     /locus_tag="P5335_01985"
     CDS             19288..19584
                     /locus_tag="P5335_01985"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRZ73680.1"
                     /translation="MTFIRFTKEAFLDIEDAALWYEHQRQGLSFDFELCLEVGIQEIS
                     RTPLAFQKKYKHVYVRFINRFPYGIHYIIKNDLITVIGVFHTSRSPKDWSSRIL"
     gene            19696..23103
                     /locus_tag="P5335_01990"
     CDS             19696..23103
                     /locus_tag="P5335_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73681.1"
                     /translation="MFKKTVLLGFLLFFSGLWAQEIETPYKKKKVTVSKDTIALEKVS
                     INKAFFKILATNDSPIDTSFYEVDFQKGTLLFKNNFQSQDTLTVRYLTFPDYLTKTYS
                     IYDDSKVVPNARGNGQLYSLSNDRINNFKPFDGLTTSGSITRGVTIGSNQNAVVNSNL
                     DLQISGKLSDKVGLRASIQDSNIPLQEGGYSQKLDEFDQIFIELFSDKWNIRAGDLFL
                     ENRQSRFLNFNKKVQGISTQFHFGNEGNKTSVFASGALVRGQYAKSTFTGLEGNQGPY
                     KLKGNNGESYVLVISGSERVFVNGLLLQRGENNQYTIDYNAGEVTFTSLFPITSEMRI
                     SIEYQFSDRNFTRFVAYGGVLHEREKWSIGGFLYSENDVKNQPLQQNLSTEQVEILKE
                     AGDDQSKMVAPSAYLDSYSENKILYRKVLISGVEAYQFSTDPLEELYNVRFSLVGNNQ
                     GNYILANNQAVGRIYAYVESIAGVPQGNYEPIIRLIAPVKIQIATVMGKYNPSEKTMV
                     DFELGLSNNDLNLYSGIDDANNQGVAGRFNGKQRLFTKQWTLDAFANYQFIQQEFRTI
                     ERLFTIEFDRDWNLTSTTGNQSLLVSGLNFSAPENGFAKYQFEKLDFSDSYSGSRHVF
                     DARYSYKKWLFQNNSSFLKSDGSYSTTSFARSDSRVKYHWNKNWAGGSFRTEDNEEKI
                     KATQSLSALSQRFSEFGGFIGRGDSTKVFVEVGYLHRVNDSLQNGFVQRVNRSHSYYL
                     NSRLLQTAKSDLSVFVNYRNLQFEDETLDDSPSLNSRILYNDRFFNQLLQLTTVYETS
                     SGTLAQQEFTYIEVDPGQGVYTWNDYNNNGIQELAEFEVAPFPDQAKYVRVYLPNQVF
                     VNTHQIKFSQALTLNPLQWQNQTGFKKFLSYFYNQTSFLVDRKTDRASGEFNLNPFQQ
                     DETDLLGLNNSFMNNLSYNRGKQNHSVTYTFVKSKSQNLLSVGFQKTINQSHQLQYTH
                     LLQKSWLFLVNAKTINSQSESENYLNRDFEILGYQFAPRVSYLFTKNASLDLFYDYQN
                     KENQMGELETLQQNRFGTSFTYAGEKQFTLNGEVSFYANDYVGDELSAVGFQMLEGLQ
                     PGRNQTWRLLIQKNLTRFLDVNVNYQGRKSETSDTIHTGNVQLRAYF"
     gene            23175..23696
                     /locus_tag="P5335_01995"
     CDS             23175..23696
                     /locus_tag="P5335_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73682.1"
                     /translation="MKSTSFLLLLAGSTFFLSSCKNSEEAPAETPAVTTEVPVPAAAA
                     EATTPETPAATATEATTGEAPKLNPAHGQPFHRCDIAVGAPLDGSGPAPASAAPTQAE
                     APKSFFKTVQSTQPAPTPAPTMNATPTPTPQPKPAPTATADANTPKPKNNPAHGQPHH
                     RCDIKVGDPLPDA"
     gene            24010..25242
                     /locus_tag="P5335_02000"
     CDS             24010..25242
                     /locus_tag="P5335_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HRZ73683.1"
                     /translation="MASIKLLLQSKNNPAVIYIRLRDGRNTDVKAKTNYHIDPELWDS
                     KEQRPSKKALKNIDYANLDTDLQEIKNNLLKEYNKSKGVVAIDLNWLNDFINPPQQEE
                     KLPEKLIDYLDKYTEYKKREVKESTLKKCGVIKQLLLRYEADRKLTLYIKNINLHFKN
                     DFENYCISQGYALNTIARAVRFIKTICNHAKANGVETHFQLDGVKAKYIKVDNIYLNE
                     TEIQQIENIESEKLTEGLENDRDWLLISCYCGQRISDFLRFDKSMIRYEKNKSGVLKP
                     LIEFTQVKTGKIMTIPLHHKIIEIVNKYGGEFPRKISDQKYNDNIKIVCKLAEIKKPT
                     HGVKFDPETKNKVEKEYEKWELVSSHIGRRSFASNNYGKIPTSFLMYVTGHTTEAMFL
                     TYIGKSNKDIAMELTNYF"
     gene            25424..26677
                     /locus_tag="P5335_02005"
     CDS             25424..26677
                     /locus_tag="P5335_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73684.1"
                     /translation="MFLRNGILELFSAKLFLDNHTPKDKNYFFNEIHKENEIRYKDDP
                     SKANLLENPDFIAYANQRAAKSVSLENEYYEISKELIKTELLIFKTKIERMGYVVHFK
                     NDDVEIIETYKNEKKNNKIQLDKKEKNTATKINNPFILNASKRGKKVLTKILFEKVAL
                     IDVIDFINKNTTLETKELLINEINGTYNLMRNEFMFKVKDEDISKALNVLENSLEYVY
                     MYKGDYERFNFYVRDFGSHKTIYNVWGLPTEYKEDTIKLVHLNNLMINYHDDFFENQV
                     EGFEQIKHLGYIVNNLKEIFIDNNAVSNNVDSKNSSIGISKDKESKMAEKWYALLYWL
                     ELLSQNKKPPTTLEGSFVRKEIENIGKNKSGKSGQGFYREFTRMSVDIKNLKSFNNSF
                     GKDWKNIIIELSNNDERIVNYLKNN"
     gene            26768..27055
                     /locus_tag="P5335_02010"
     CDS             26768..27055
                     /locus_tag="P5335_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRZ73685.1"
                     /translation="MQTKSILLENLTVDQLQQLIGTSVRNGIFELQKQIQTQSDNEAL
                     MTRDETCQFLKVDSSTLWAWTNQGKVKAYGIGARRYYKKSELLECLTLLKK"
BASE COUNT         8711 a         4803 c         4647 g         8816 t
ORIGIN      
        1 accggtagct ccttaaaatt tatgatggat gcttttccgg aaagagcttt tgatgtaggt
       61 attgctgaac agcatgcagt gacattatcg gctggaatgg ctacccaagg aatggtggtt
      121 ttttgcaaca tttattctac ttttttacaa cgggcatatg atcaggtgat tcatgatgtt
      181 gccttgcaaa atcttccagt cattttttgt ctagatagag caggattagt aggtgaagac
      241 ggagctactc atcacggtgt ttttgattta gcctacttgc gttgcattcc gaatatgatt
      301 gttttttcac ccagaaacga aattgaatta cgaaacatgc tttataccgc tcaattagga
      361 ttaaatcatc cgattgctat tcgttatcca agaggtagag gagaaattgt cgattggcaa
      421 attccctttc aaaaaacaga aattggaaaa gcaagtttgt taaaaaaagg atcaaaagta
      481 gccatcttgt ccaatggttt tattggaaaa aatgtgacca ctgctttaac tttaatttca
      541 gctcaaaatg atttttctca ttatgatttc ccttttgtaa aaccattgga tgaacaactt
      601 ttaacttcaa tttttaagga tcatgaaagt attatcacct tggaagacgg aacaattata
      661 ggtggatttg ggtcaggaat acttgaattt aaagctaaaa acaagtacaa aaatgaggtt
      721 caggttttag gaattcccga tgaatttatt gaacagggaa ccatcgatga actacaacgt
      781 tttagcaaaa ttaacgtgga aaatttggtc tccattttgt caacttattc aaaatagtga
      841 cttttgcaaa agtcttatta ctattttgtc tatgaaatct atttcatcca ctttattttt
      901 attactctca tcttccgttc ttttttcaca acctttacaa acagtgaaag attctaccct
      961 taaagatact acctattggg ttcagaaaaa aatagtggga ttagacatta ctcaaattgc
     1021 atttctaaat tggaatgccg gaggaaacag ttctatttca ggtttattta aaggtgattt
     1081 ttcgagaaaa tataccaaag aaaatgtctt atggaataat gaaatgagca tccgatacgg
     1141 catcaacaaa caagatgatc gagagctacg aaaaacagat gatgcctttt cgattctttc
     1201 aaattacggc tataaaacgg acattaattc caattggtat agttctgccc ggtttaattt
     1261 taatactcaa tttacgaatg gttatgccta tccgaatata gatactccaa tttcaaagtt
     1321 ttttgctccg gcttatgttt ttttgggaat tgggagtgag tataaccgaa aagattggaa
     1381 tgcaaatttt tatttttcgc ctttaacttt aaaaaccacc ttcgttttaa accaacgttt
     1441 agccgatttg ggttcgtatg gagtgacaaa agcgatttac gatgaagaag gaaacctatt
     1501 aaaaagaggt cgaaaaagcc gaacagaatt aggtatgctt ttcaacaatt actggaaaac
     1561 ggaaatctac aaaaacatga cattagaaaa cagattgtca ttatattccg actacctgaa
     1621 caattttggg aacattgatg tggactggca attacaagta gatatgattg taaatgaata
     1681 tgtgaaggct aatattggca ttcacatgat ttatgatgat gatattaagg ccaaagaaga
     1741 agtaaatgga gaacaaatta cggttggccc caaacttcaa ttaaaacaag ctttagggat
     1801 tggcgtagtt tatgtttttt aaaataaaaa aacccggaag attccgggtt ttatatatta
     1861 aaatctcatt aattagtttt taataatctt ctgagaaaag tttaattcat ctgcatttac
     1921 tttcactata tacattcctg attgaagatg tgttaaatta atatttttag cattattaaa
     1981 attaacttca ttttcttggt aaacgattct accatttaaa tcaacaattt gaattgccac
     2041 ttttccagag aattgattta cttgaatagt aaacattcca ttggaaggat ttggatatac
     2101 tttaaatact tcattcgttg caaaataatc tactgataaa acacagtttg gtccagctgg
     2161 tggcattgta aaatcttcca cttgatcggt agaactattt ctatttccag aagaggcatt
     2221 taaacctaaa cctctattgg caaacacttc ccaaatcata caataatctt gacctccggt
     2281 agtagcttgg tcagctgcaa ttaatgcatc tcttgcctca ataaaagttg gagaacaagg
     2341 ttgcaatttt aatccatcaa gtactaaacg cataatttta ttgtttcctc cagtaccggt
     2401 atatatattt gaatcataac catatttatt aatatatgcc catgtcaaat cccataaagt
     2461 agttgcccaa acagaaccaa caccatgagg aaccgcttct gtattcgtac tattgaacgt
     2521 cattgggttg atactcatat cggttgaata cggataagtt ctaattccaa ctccatcaat
     2581 tggctggcta actgcaaaag ttccaatccc tctaatagca gtgcctacat ctccaggttt
     2641 caattgcatc attaaagcga accaatcgga ccaaccctca cccatttgtt cagcattttg
     2701 taaacaacta gaattgtttc tacctcctgc taatcgaata gagattccat gaccaaattc
     2761 atgagaaata attccattat caaaagaacc atctgcattg ataaaaacat cagccggggt
     2821 ttgaagtgtt acattaacta cacctaaact catttgagct ataatagctt cacctacatc
     2881 ttgatttata ctgattgcag gaatagttat tgttgcatca gcaccaccca tgataataac
     2941 tccttcaaca ttattgacaa ctattacagc aagagctcct gcattttgag cattcttaac
     3001 tttttcaacg aaagtacaat ctcctcttct caaaatacaa actttacctg aaatagcagc
     3061 agcattaatt gccggagaac aagcatccga attatccgga gatccatcat caaaaagcac
     3121 taaatcagtt gtgaaaccgt ttggagcaat tggtagtgga acattaccag ggataaaatt
     3181 attatcacga attgcatatg gaccagcaat agtagcaggt gtatttacgg ttaaagaata
     3241 atttggcgga cctacatccc ataaaaacat ttgaactctt ccagattgtc catcaacggg
     3301 tgctgagaaa ttagcattgt ttgtaccaac tccatcttga gaatctgcat atacaaaatc
     3361 accggctgta ccgccttttc cataattatt ggcttggtaa tttccatttg cttcatcaaa
     3421 accatattga taccaaacat catgcatcac attggtcata taaaataaat tggttgtagc
     3481 tgcatttgta taggtagttg attgtgcact attcccacca taaggaaaat caaactctaa
     3541 aattgctcca ccatttggtg ctgcaccgaa aacactatta gttccatttc ggtcttcttt
     3601 tgcccaggta ttatttcctc tggtaatcgt aaattcagct ccatctacac cgttagtatc
     3661 gtgccatcca tatggtgaag ccgttgcgtt gtgtggagta gaaattaatt gtctttcacc
     3721 gtggttcgga ctttcaatat tgtatggtat aacgcgataa gatcccgatt gtgtctctac
     3781 taccgaaatc gctgattctt ctttaaacaa ttgtttagtg aaaggaacat aatgttgatg
     3841 cttcgagtga tctgaatctt ttccgaaact acaactaaca accatgtctt gtttttcaag
     3901 aatttcgccg tttttagcat caattctaac gctccataaa tttttgtgat ttggactgta
     3961 aaaagtaaaa tcccaagcta atctgagttt attttcttca tttaattgat aaactagttt
     4021 agctaaaaca ggctcattga tgccaattgc atcatttatc tgataataat tagttctgat
     4081 tgttctttga atttgaaagg attcaattgg ggtgatatta aaactaccat acaccaaact
     4141 caatgcatcc aatatactgt aagccggtgt agttgtattt actcgttgtg caatattgct
     4201 ttcaaaacgg ttagcaacat aaactacatt tccgtttttg attgaaaaat ttgtttgagc
     4261 atgaaatatt tcaattcctt gataacgttg tttaacgtaa taattagtta tcccggtgga
     4321 agaggaagtg gcttcacttt ctacatacca atcattgata tcttgtgatg ttatacctag
     4381 tgtagaataa tttgaattta aatattcttg aattttttgg gtttcagttt gtgcattaac
     4441 tgaaaaagca aacaaaacaa agaataagaa tgtaattttt ttcataaaaa agtgaaattt
     4501 ggaaatttaa gagccaaata tataattatt ttcaacaatt aggtgtgttt tttaattact
     4561 ttaactattg ttagaagata ctattttgtt gagtaatatt gcgttaccat cagttaataa
     4621 tgaaatataa tgtgaaatat gtaacaaccg ctcatacaac gtctctttat tcacttggta
     4681 tttttcaggc aatactttta acaataaaac atcataatta gacattttac catggtattg
     4741 attattgaat gctgttataa atttatccaa taaattattg ataacgtggt agccggtaat
     4801 ctccttttct ataacttcac gactttggta aattttttta acactcaatt tgataatatc
     4861 atccatctgg gctttgtatt tgcttttatc tgttaaggca aaaggaaatt gtccttttaa
     4921 aatatgttct tcattgtcaa taaaaatccg aacagcatca ttaattaagc ttccaatggc
     4981 taaagcacgt aggtaactaa ttctatcttc cttagtttgt aatgaattgt atttagaaac
     5041 atcaatgctg tcttttacta acttaatcaa gtactctaaa gcaaaatctt cattcactaa
     5101 acctaaattt attccatctt caaaatcgat aatggtgtaa caaatgtcgt ctgctgcttc
     5161 cactaaataa gctaaaggat gtctttcata gccaatattg tcgctggttt tattagaaat
     5221 taatcctaat tcttccgcta cttctttaaa aaaagctttg tcttgttgaa aaaagccata
     5281 ttttttatcg gcaattgcag tagttggttt tttgggtaaa ctttctttgg gatatttcat
     5341 aaatgccccc aaggttgcat acgaaattcg taatcctcct tcatttccgg gacgatttcc
     5401 ggttaaaaca gaaaacccat ttgcatttcc ttcaaaatca attaaatctt gccattcttt
     5461 ttcagcaagt tgatttttat attgctgtcc gtttccggtt ttaaaatatt cgccaatcgc
     5521 cttttcaccg gaatgaccaa agggaggatt tccaatgtca tgagctaaac aagcagcggc
     5581 tacaattgct ccgaaatcat tcatgtgaaa accatgaatc tctttgagat agggatattt
     5641 ttcaatgatt tttttcccga ctaatcgtcc aatagatcgt ccaacaacag atacttcaag
     5701 actatgtgtc aatctggtat gtacaaaatc tgttttagaa agtggaataa cttgtgtttt
     5761 atcttgcaga cttcggaatg ctgcagaaaa tataatacgg tcataatcca cttcaaagcc
     5821 taaacgggta tcgtcttgtt cagagcgtaa acgtttcccg gtgtcgcctt gtcttttgag
     5881 cgataaaagt tgttcccagt gcatcatgtt attcttgtcc aatttcgtta atagaatctt
     5941 gcatttctaa ctcctgatta tattttttta aatcgaccac aactgtttta gagagggtat
     6001 catgccaccc tctgggtatt gttcctaaaa aagataattg atcaaaaggt atcaatcggg
     6061 atagtgagcg tataaagata gagtaaggtt cgggtttaga accatctgcc atcattactt
     6121 ttgtattggt gacatatttc ccaattgatt ttccggttaa cgattcaaaa atcatgtaat
     6181 aaatcacaat aatcaaataa ctaatcagga aatcaactat tggattgatg gattctaacc
     6241 aaagggaaag ttcatagttt ccaaaaaatt catataaaat ccaacctatc ccaattggaa
     6301 taacattgat aatattatga ataaccatat caattaaata atttcccaat cgttgtccgg
     6361 atgaggctaa catggctgat gtaatcgcaa tttcttttgg tttgacaaac tccatacttt
     6421 actttaaatt ttacgctttt ttatcgtttg ccaaaaagaa atttcttgat ttttttgggt
     6481 attgattgta gctgatatca cttggatttt caatcaccgt tttgatattg attaaatctc
     6541 ctaatttaaa atttgcttcg gtatatttaa aatctaataa atattccccg cagaaatagt
     6601 ttcctttttc atctttttcg atgatgcctg tataaacgcc ttttttatct tttgacataa
     6661 ttttgaaatt actttttcaa atgtacgaat tgaaattgat taatataaaa acagccatcg
     6721 attgacggct gtttttctta tgtaatgttt tttaattatg atccacacat ctcacaatca
     6781 tccggtcctg catttcttgc tttttcgacc atagctttga aatcttctgc cgtcatttct
     6841 ccagtttcat ttggagcaaa aacttccact acttgtggtt cgggtgttgg ctccgctttt
     6901 ttgtcattat ttaaggtaaa cttaattgca tctactgccg atttagttct taaataatac
     6961 attcctgttt tcaatcctga ttgccaagca tagaaatgca tcgaggttaa tttagcatag
     7021 gttgctcctt ccataaacaa gttcaacgat tgcgattgat caatgaaata acctctttga
     7081 cgcgacatgt caatgatgtc tttcatgctc atttcccaaa ctgttttgta caaatctttg
     7141 atatcctgag gaataatttc aatattttga actgaaccat tgtgacgcat gatttcttgt
     7201 ttcaaggtgt cgttccataa accgagattt actaaatctt ctaacaagtg tttgtttact
     7261 acaataaact caccggaaag tactcttcga gtgtaaatat tggaagtata aggttcaaat
     7321 gcttcgttgt ttcctaagat ttgtgaagta gaagcagttg gcattggagc gactaacaat
     7381 gagtttctca caccgtgttc tactacttct ttacgtaaac tgtcccaatc ccaacgccct
     7441 gataaatcgc tgtcttttaa tccccaaaga ttgtattgga attctccttg cgaaatggga
     7501 gaacctttaa aagtggaata cggaccttct tcttttgcca tttccatgga tgcggttacg
     7561 gctgcaaaat aaagtgtttc aaaaatatct tgattcattt gttttgcttc gtcgctggtg
     7621 aacggtaagc gaagtaaaat gaacgcatct gctaaacctt gcactcccaa tccgatagga
     7681 cggtgacgta tatttgaatt ttccgcttcc ggaaccggat aataatttcg atcgattaca
     7741 cggttcaagt ttcttgttac tcgtttggtt acatcgtata acttctgatg gtcaaatttg
     7801 ccgttttcaa taaacattgg caacgaaata gaggccaagt tacaaactgc aatttcatca
     7861 ggtgatgtat attctaaaat ctccgtacat aaattcgatg aacgaatggt tcctaaattc
     7921 ttttgattgg atttacgatt ggctgcatct ttgtacaaca tataaggcgt cccggtttcg
     7981 atttgtgatt ctaggatttt ctcccaaagt tcacgggcac gaacggtttt tctacctttt
     8041 cctgcagctt catatttgtg gtacaacgct tcgaattcgt cgccatacac atcacaaaga
     8101 cccggacatt catttggaca cattaacgtc catgaagcat cgtcttgcac gcgtttcata
     8161 aataaatcgg atgtccacat agcaaagaat aaatctcttg cacgcatttc ttcttttccg
     8221 gtattctttt tcaaatctaa aaattcgaaa atatcagcat gccacgtttc taaataaatg
     8281 gcaaaacttc cttttctttt tccgccacct tggtcaacgt aacgagctgt gtcattgaaa
     8341 acacgtagca ttggaacaat tccgttagac gttccgtttg tcccacgaat ataagaacct
     8401 gtagctctca cattgtgaat tgaaagaccg attcctcccg ctgattgcga aattttggcg
     8461 gtttgtttca aagtatcata aattccgtca atgctgtcgt ctttcatggt taacaagaaa
     8521 cacgaggaca tttgcggttt tggggttccg gcattgaata aagttggtgt cgcgtgtgta
     8581 aagaactttt tcgacattaa ttcgtaggtt tctttcactg cttcgatgtc gttcaaatga
     8641 attccaaccg aaacacgcat caacatatgt tggggtcgct ctacaatttt tccgtttatt
     8701 tttagcaaat acgaacgttc taatgttttg aaaccaaaat aatcataatt aaaatctctg
     8761 ttataaataa tcatagagtc caattcttca gcattggcca taatagcatc atacacttct
     8821 tctgaaagta aaggtgattg ttgtccggta cgtggattaa cgtaatggta catatccgac
     8881 atcgtttctg aaaacgattt ttttgtattt ttatgtaagt tggaaacagc aatacgagct
     8941 gctaattgtg cataatccgg gtgggcaatc gtcatcgagg cagcagtttc agctgctaaa
     9001 ttatccaatt cagaagttgt tacaccatcg tacaatcctt caattacacg catggccact
     9061 tttacagaat cgactaaatc atttaaacca tagcatagtt ttttaattct atctgtgatt
     9121 ttgtcaaaca ttaccggttc tttgtgaccg tctcttttta ctacatacat acacttacat
     9181 gttttttaaa acagaaaatc ccttttactg ttttgggtta tttgttagtt ggttttggga
     9241 agctaatccc ggtaatctta aaattaactc cgtcagaatt taaactgtgt taaaaatcag
     9301 cgtcgaaact aattttttgt gaatcgctat ctgaattcat cacgcctgct ttttggtatt
     9361 ccgctacacg tttttcgaag aaattggttt ttccttgtaa tgaaatcata tccataaagt
     9421 caaacggatt agttgaatta taaactcttt tgcaccctaa ttcgaccaat aatctatcag
     9481 taacaaattc taaatattgt gtcatcaatg tggcattcat ccctatcaaa cttaccggaa
     9541 gggattccgt aatgaatttt cgttctattt ctaaagcatc agtcaatatt tccgtgattc
     9601 tggcttttga aactttattt acaatatgat gggtatgcaa atgcacggca aaatcacaat
     9661 gcattccttc atcacgagaa attaattcgt tagaaaaagt taatcccggc attaatcctc
     9721 tttttttcaa ccaaaaaatg gaacaaaacg aacctgagaa aaatattcct tctactgcgg
     9781 caaaggcaat taatctttca gcaaacgaat ctgattctat ccatttcaaa gcccaatctg
     9841 ctttttcttt tatcgcggga aacgtttcaa tggcatgaaa caacatatct ttttctgctt
     9901 catctttcac ataggtatca attaataagg aataggtttc actgtggatg ttttccatca
     9961 ttaattggaa accatagaaa aactttgctt caggatattg tacttcactc acaaagtttt
    10021 cagcaaggtt ttcattaact attccgtcag aagcagcaaa aaaagctaaa atatgcttta
    10081 taaaatattt ttcatcagcg gataatttgt tattccaatc gtttaaatcg gtatgcaaat
    10141 caatttcttc tgcggtccaa atgcaggctt cttgtttttt ataccattcc caaatgtcct
    10201 ggtgtttaat cggaaaaatt acaaatctat ctttattttc ttgcaaaatt ggttctactg
    10261 ctgccatctt tttctttgtt aagttatgtt gaggattatt aattcgtatt tgagagttac
    10321 aaagattgca attcaaattg gtaaatgaaa gccaaactta ttcacaatcg gagttagttt
    10381 ttaacaacgg gtaatcggtt tcttacgttt aaaaaatgaa gataactaac tgtttattaa
    10441 cgtaataaaa acagctttat tggtggtcgc aagcaaaatg gaatgctttt taagaaaata
    10501 gtttttagaa aattaaatga atttctattt ccctttcagg tattcttccc ctactttttc
    10561 aagttctgtg aaccactctt ccccgaattt tcgaataaga gcttctttga caaatttata
    10621 aaccggaact tccagttctt tccccaatga acaagcggga ctacaaatat cccaacgatg
    10681 gtaattaacc gctgagaaat cagagaattc tttgattcga ataggataca aatgacacga
    10741 aactggtttc ttccagttaa caattccctg gttatacgct tgttcgatac cacaaagtgc
    10801 tgtcttaccg tcaaagatta cataagcaca atcttgtccg ttgatgagtg gtgtttcaaa
    10861 ttctccgtct tcgcctttta cccaggttcc ttgtgcttca attgctgcaa ctccttcttt
    10921 gcgaagaaag ggtttcactt tgggataaat atcttctaat atcttcgttt cttcggcact
    10981 cagtggagca ccggcatcac catcaacaca acatgctcct aaacaagctg ttagattaca
    11041 tacaaaattt ttctctaaaa tttcttcaga aacgatggtt ttgcctaatt gaaacatggt
    11101 aaaaggtata gaatatagcg gcaaatgtac aaaggattgt ttgatactac gattgaaatg
    11161 gaataaataa ttgacttttt cgacttatat agattacttt tgcctcaact atttatttgt
    11221 tatggaatta gatttaagag aaatttttac ggctacaatg gtattatttg ctgtaattga
    11281 tattgtaggt agcattccta ttattatcga tttacgcaat aaagttggcc acattcattc
    11341 tgaaaaagct tcaattgtag cttgtattat gatgattgca tttttatttg tgggagaaac
    11401 tattctaagt ttaatcggaa ttgatatcaa ttcgtttgcc gtagccggtt cgtttgtgct
    11461 tttcttttta gcattggaaa tgattttagg aataaaactt taccgcgatg aacaaaatga
    11521 atccacttca attgttccta tcgcttttcc gttgattgcc ggagcaggaa cgatgacaac
    11581 tattttatct attcgagctg aatatcaaac cataaacatt ataattgcca tcattttaaa
    11641 tattatttta gtttatggtg ttttaaaatc atctgccaag attgaacgat tattgacaaa
    11701 aaatggtttg agcgtcatac gaaaagtctt tggtgttgtc cttttagcta tcgctgttaa
    11761 attatttgct gctaatgtta aaggattgtt cgaataaatt gatttttaat acttttgttt
    11821 ctttaaatcg taacgatgaa actgtttact tctattttgt tggtgttggc ttttggatta
    11881 atcattttca acttaacaca gttagattat ggtaatcttt ttgcacaaga aagtatgatt
    11941 gccttgattg gtattgtagc tgctttttgt gccattttga ttttactcat ctttagaatg
    12001 tcaaaaacta ttgaagacaa actaaaagac caagaataaa tgtttgattt acttgtcatt
    12061 ggtggaggag tatcaggggt gtcttgttcc cttttgcttg gttctgctca aaacaagcct
    12121 tttgcttcgg ataaaaaaat cggactcatt gcccatcaaa aagcttcctc gcttcaagaa
    12181 gctttgttta ataatgctta cggaatttct cccggaaaat tagggagtga tcttttaagt
    12241 gaaagtttag ctcatcttca atcaacctat ccacatattc agcaaattcc aaacgaaaaa
    12301 gtagttaaaa ttgatggtga tgccggaaat tattccgtta caaccaacaa aaacacgtac
    12361 caaacaaaat taattgttat cggcattgga gccggaaatc cttttacgat tgaaggcttg
    12421 gaagaatttg tgattccaca tcaaaaaata aatccggaga aaaaccgtat tcaacttaaa
    12481 aataacgatc atttagttaa agacggtatt tatgtttgtg gaactttagc aggttggcga
    12541 agtcaattac ctattgcggc aggaagcgga gctgcagtag cgacagacat tttaaccttg
    12601 tggaatgatg gaaatcatgt tcaggtgcat gatagcacta aagtgaagta ggtttctttt
    12661 gtcggtttct aatttattgc ttcaaaactt ccaaaatcat tgaatccttt tttaacctga
    12721 tttcaaaata tttttcttca tcaaataatt gtcttgaaaa ttcagctatg atgtaatgtt
    12781 tgaccattgc tttatgacta cttaatgaaa aggttacacc gcttgatttt aaataatcct
    12841 gaaaacgttg cacatataaa tctgttgttt ccgctttttt ataaaacgtt tcaaaagaaa
    12901 ttccttcaaa ttcagtgcgg tttttgtcta tttgttcaaa aacaaaataa ctcaccaccc
    12961 ccgattgcat taacatttcc aaagcttcat caccgtgctt tccttgtaaa ggcacaaaaa
    13021 tatccggaat aattccaccg ccgccataaa ctgttctccc tttgagtgtt ttatatttta
    13081 agctgtcggc tactttaatg ctgtcttttt catacaactc accattataa aaacgttttt
    13141 caaactcgtt gaaataatct tcatttcctt caacataagg tttttggatt gaacgtccgg
    13201 aaggggtgta ataccgagca acagttaatc gcaccgcaga accatcgcct agaggcattt
    13261 cgcgttgtac taatcctttt ccaaaagagc gtctgcctac aattaatccg cggtcattat
    13321 cctgaatagc cccggccaaa atttctgagg ctgaagcgga gttttcattt attaaaatga
    13381 tgacttttcc attttcaaaa aggccattat tggttgcttt tgttttatca acccttcctt
    13441 ttttattttt ggtcgaaaca ataggtgcat cctccactaa aaaatcatca gcaatttgaa
    13501 ctgcttgatc taaataacct ccaccattat cccgaacatc taaaacgata gtttttgctc
    13561 cttgtttttt tagttttagt aatccttttc tatattcctg aaaagtagat tctgcaaaac
    13621 gattaatttt aatataaccc gttgttgggt ttatcatcaa ggaaacatct acgcttttaa
    13681 tggggatgac atctcgtttt agtttaacgg taagcttttt attttcactt tttcgataaa
    13741 ctgtcaaggt aacttctgaa cctatttccc cttttaattt agaatataaa gagtcatttg
    13801 gtagtttttt cccaaaaagc ttgtaattgt cggcaaataa aatacggtct cctgatttga
    13861 taccggcttt ttcagagggt ccaccgttta agggtttaat aactgtaatg gtatctttgt
    13921 acatgtagaa atttacccca attccaacaa aatcaccccg catggattct gatacttggt
    13981 ttaattcatt tttggtaata tagacagaat gtggatcaag tttttcaaga atgccattaa
    14041 ccgttaaatc tactatactg tcggtatcga cttggtcaac gtaatcccgt tcaataaaat
    14101 cgattagttt gttcagttta cttttataac taccggaatt gaaagtcgtt ttatttgaaa
    14161 aataattgag ttttcctcca atcacaacac ctaatgcaat ggcagaaaag agaacaattg
    14221 gccaatatat tttttttatt ttcacagttt atttttcggc taacggaaaa aatagttatt
    14281 ctaaatctgt tattaattct acttcaacgc cggcttttaa taaaaactgt aatccgctgt
    14341 cgtctttgta tccttcatga taaaccaccc gttttattcc ggattgatgt atcagtttac
    14401 tgcattcctt gcagggtgac aacgtaatat agagtgttgc tccttcacaa gattgagtag
    14461 aaccggccac ttttaaaatg gcattggctt cagcgtgtaa aacataccat tttgtcgttc
    14521 cgctttcctc ttcacaacag ttttcaaatc cggaaggagt accattatac ccatctgaaa
    14581 taatcattct atctctcaca atgatggctc ctacttgttt gcgtttgcaa taagacaatc
    14641 tactccattc tttcgcaatg cgaaggtagg ccttgtcgta tttatttaat ttctttttat
    14701 tcatttttta acaactgttg caaggaattt atctattcca aatttcacta ttaataatca
    14761 ttggcaacac aacacctatc acaaaagatg acatcaccaa ggtccaatcc cgttttgaaa
    14821 aacggaaagc tgtttgagta acataggaca aaagtaaaac aataaaaaca acaataagct
    14881 gagctgcttc aattcctaac gcaaattcta gtaaaggcag taatttttcc gaactatttc
    14941 ccggtaaaat agttttgaaa taattcgaaa agccaagccc atgaataatt ccgaagaaga
    15001 tagttataaa tgcaacaaaa gtgatgcttt ctttttttcc ggatttgcct gccgtgaata
    15061 agtcaaataa agccactatc aatatagtaa tcggaattag aaactcaact aaattagctt
    15121 taatcacaac cactccataa acggaaagca gtaatgccaa ggtgtgccct agcgtaaaaa
    15181 gcgacactaa aatcaagact cttttccaat ctttaaaggc ataaggaacc gttaaagcta
    15241 ttagaaacaa tacatggtca taaccattcc aatccaagac atgtttgagt ccaatctcaa
    15301 aatacaacca aaattcagac atcgtatatt aatttaaaat ttagggaatt caaacttaca
    15361 atatattttg aaacgcttct cacaaaaact ttaaaattga tgtttagagt gtacttttcc
    15421 gctaaatatt ttaaacaaat aggcggaaaa atttgaaaat agaattatct ttgtagtata
    15481 gataaacaca aaaattatgt cattttcaga tttatttgat agcggtttta aagaaagaaa
    15541 taaaggttat ttctcttcat ttgttcgtat tgcactttct gacaacgtcc ttacggatga
    15601 tgaaaaaaaa ttcttagacc gtattgcgtt aaagttggat atctctccgg aggaatacca
    15661 ggaaatcctt gaaaatccgg caaaatatcc tgtcgatcca ccgtatttat acattcaacg
    15721 tttagaacga ttatatgatt taggccgtat ggtttatgct gatcacgttt tagggccaag
    15781 acaaaaagat attttaacta ggtatgcttt atatttgggt tttactcccg taaatgtaac
    15841 ctatattgtt gacaaagctc tcgcactttt agttcacact gttgacttag atacgttcat
    15901 ttttgaaatg caacacatga ataagtaaag aaaaagccct gaaattcagg gcttttttat
    15961 tctatctttt ttatttatac ttttccataa actcttctgc tttctccacc atttttaagc
    16021 ttccgcaaaa gaagggaacg cgttggtgta attcagaagg ttgaatttcc atgatgcgac
    16081 tataaccgtc tgaagctctg ccgccggatt gttctgccag gaaagccatt gggttacact
    16141 catacagtaa acgtaatttc ccattaggtg cttttgaact agttgggtaa atataaatac
    16201 caccttttat catatttcgg tgaaaatcgg agactaaact tccaatgtat ctcgaggtgt
    16261 aaggtctgtc ttcttcttcc aactgacagt atttgatata atcttttacc ccttgtggaa
    16321 aatgaacata attgccttcg tttattgaat aaatagttcc tgtttcagca tatttcatat
    16381 tcggatgtga aagataaaat gttccgatag ccgggtttag tgtaaatcca tttacgccgt
    16441 gtcctgtagt atacaccaac atggttgatg ttccataaat cacataacct gcagctacct
    16501 gattaattcc gggttgaaga aaatccttta aagttaccgg tgtgccaatc ggtgttattc
    16561 gtcggaaaac agaaaaaata gttcccaccg aaacattcac atcaatattg gatgagccat
    16621 ctaacggatc cattaatact acatatttgt tgttatgact gttgtcgctt ccggctacgg
    16681 tgataaaatc atcgttttct tccgaagcaa ttccgcaaac aatttctcga ttaataagag
    16741 tttgaataaa tacttcattg gcataaacat ccaatttttg ctggtcttcc ccttgaatat
    16801 tttgttcacc ggcagctcct acaatgtcaa ctaatccggc tttatttacc ttatagttga
    16861 ccacttttgc cgccaaacgg atggaattaa tgattctcga aagctctccg gttgagtact
    16921 gaaattcttc ttgtttttcg atgataaatt caccgagggt tgtgtttctt ttttccatta
    16981 cgaaaacgtt gtagttgtat ttgtggcaca aatatcgaaa tttttgtgat acgaatgata
    17041 attagtttaa aatgaattta gaattgtatt tttgttgaaa tattttaccc gatgattatc
    17101 cgaaaaggaa cacccaacga catgccttca gtgctcgaat taataaaaga attggctatt
    17161 tttgaaaaag aacccgatgc tgtagttgtt acggtgaatg atttagtacg ggatggtttc
    17221 ggtgaaaagc ccttatttta ttcgtatgta gcagaggtaa aggatgaaat tattggaatg
    17281 gctttgtatt attatcgcta ttcaacctgg aaaggcaaaa ccattcattt agaagatttg
    17341 attgtaaaag aaaatcaccg tggttcaaga gcaggattgg ctttatacac tgcaataatt
    17401 gaacaaggta aaaaagactg tgtccgacga atcgaatgga atgtattgga ttggaattta
    17461 ccggccattc agttttacga aaagtcagga gcaaaagtat tgactgattg gcgtgttgcc
    17521 catatggatg aagaaggaat taagcgattt ttgaatgata aataacgata tttgaaataa
    17581 agaaattaat tgacctgtta acgcatgaaa atattcaaat ttggtggagc ttccgttaag
    17641 gatgccgacg gtgtaaaaaa cgtattgtct gttttagaaa aagtaggcta tgaagatgtc
    17701 ttgttagtca tttccgcaat gggaaaaact accaatgcct tagaagttgt gattaaaaat
    17761 tatttcgaca aatcagaagc cttgaatgct tctgttcaag aggttcgaaa ataccacaat
    17821 cagatattgc tggaactttt tgaagacgac aagcatcctg tttttaaagc catcaattcg
    17881 cattttgaag atttggatta ttttttaagt cataataaat cgcccaatta taactttgtc
    17941 tatgaccaag tggtgagtta tggtgaattg gtttcgacta ccatcgtgag tcattttttt
    18001 aattacagtg gcctaaaaaa caattggatt gatgtgcgtc agttcataaa aaccgatgcg
    18061 acttaccgcg atgctttagt ggattgggaa caaacgcaaa agcttatttc aaaaggggta
    18121 aagaaaaaag ccttaaacat cacacaaggc tttttaggtt ctgatgaaaa caactttaca
    18181 acaactttag gtcgagaagg atccgattac acagcagcca tatttgccta ttgtttaaat
    18241 gccgaaagtg tgaccatttg gaaagacgtt cccggcgtat tgaatgccga tccccgttat
    18301 tttgaaaatg ccattttgct aaaccagatt tcttatcggg aagcgattga gttagccttt
    18361 tacggagcga ctgtgattca tcccaaaaca ttgcaaccct tgcaacgcaa agaaattcca
    18421 ctgtttgtta agtcatttgt gaatccgctg ttgccgggaa caaaagtttc aaaaggagct
    18481 gatttagaac cgcatacttc ctgttttatt gtgaagaaaa accagctgtt gatttcactt
    18541 tcttctattg atttttcgtt tatcatggag gaaaacatca gcgagatttt tgccttgttt
    18601 catcactata aaattaaagt cagtttgatt cagaataccg ctattagttt ttctgtttgt
    18661 atagaagata aatttggtca tttccctgag ttaaaaacga tactttccaa aaaatttaaa
    18721 gtgtcgtata atgaaaatgt atcgctctat accattcgtc attttactga aaaagccgca
    18781 caaatggtag agcaaaacaa aacggttcta gtgaaacaaa ttagtcgaga gacgatgcaa
    18841 gtggtcacta aggaatgagt tttgtttgat gtttaagact nnnnnnnnnn nnnnnnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18961 nnnnnnnnnn nnnnnnnnnn cgagagacga tgcaagtggt cactaaggaa tgagttttgt
    19021 ttgatgttta agactttaaa gtgatacgaa aaagattttt tattattttt atataaaaaa
    19081 catgaaaatt aaagatatat caaaattcag taccgctgaa aaaattgttt tagctgagca
    19141 attatgggat agtatttcta aaaaagacat atcgattact gaggaaatta agaaggaatt
    19201 agatcatcga cttaacttgg tagaagaagg aaaaaccgag atttacagtt gggaagagat
    19261 aaaagctaat ctggaaaaga gtagaaaatg acctttattc gatttacaaa agaagccttt
    19321 ttagatattg aagatgcggc tttatggtat gagcatcaac ggcaaggact ttccttcgac
    19381 tttgaacttt gtttagaagt tggtattcag gaaatttcga gaacacctct agcttttcaa
    19441 aaaaaatata aacatgtcta cgttcgattt ataaaccgct ttccctatgg cattcattat
    19501 attattaaaa atgatttaat aacagtcatt ggtgtttttc atacctcacg ctcgcctaaa
    19561 gattggtctt cccgaatttt ataattccct gtttatttac tttcatttcc ctttcctgat
    19621 tccattgcga ctcaaataat ccctgattct ttcacatata aatactactt ttgagctttt
    19681 agagttatag tatttatgtt taaaaaaacg gttttactgg gttttctact gtttttttcc
    19741 gggctttggg ctcaggaaat agaaacccct tataaaaaaa agaaagttac cgtttctaaa
    19801 gacacgattg ctctggagaa agtgagcatt aataaagctt tttttaaaat cttagcaacc
    19861 aatgacagcc cgattgacac ctctttttat gaggtggatt ttcaaaaagg aaccttactt
    19921 tttaaaaaca actttcaatc gcaagatacc ttaacggtcc gttacttaac attccccgac
    19981 tatttgacca aaacctatag tatttacgac gacagcaaag tggttcctaa tgccagggga
    20041 aacggtcaat tgtattcgct ttccaatgat cgcatcaaca atttcaaacc ttttgacggg
    20101 ctcaccactt ccggaagcat cacccgtggg gtgaccatcg ggagtaatca aaatgccgtg
    20161 gttaattcta atttagacct tcaaatctcg gggaaattgt ctgataaagt gggtttacgg
    20221 gcttctatac aagacagcaa cattccgttg caagaaggcg gttattcgca aaaactggat
    20281 gaatttgacc agatttttat cgaattgttt tctgataaat ggaacattcg tgccggtgat
    20341 ttattcttag aaaacaggca atcaaggttt ctgaatttca acaagaaagt gcaaggtatc
    20401 tcaacgcaat tccattttgg caatgaagga aacaaaactt ccgtatttgc ttcaggagcg
    20461 ttggttcggg ggcaatatgc caaaagtacc tttacgggtt tggaaggcaa tcagggaccc
    20521 tataaattaa aaggcaataa cggcgaatcg tacgtgttgg ttatttccgg ttcagaacgt
    20581 gtttttgtga atggcctttt gttgcaacgc ggtgaaaaca atcagtatac gattgattac
    20641 aatgccggag aagtaacctt tacctccctt tttcctatta cgtctgaaat gcggatttcg
    20701 attgaatacc aattttccga ccgcaatttt actcgttttg ttgcttacgg cggagtactg
    20761 catgagcgtg aaaaatggag tatcggtggg tttctgtatt ctgaaaatga tgtgaagaac
    20821 caacctttac aacaaaatct ttctacggaa caagtggaaa tcttaaagga agccggagac
    20881 gatcaatcta aaatggttgc tccatcggcc tatttagaca gctattctga gaataaaata
    20941 ttgtatcgaa aagtacttat ttccggggtg gaggcttatc aattttctac tgatccgtta
    21001 gaagaattat acaatgtacg cttttcactc gttgggaata atcaagggaa ttatatcttg
    21061 gcgaacaatc aagccgtggg acggatttat gcctatgtgg aatctattgc cggcgtaccc
    21121 caaggaaatt atgagccgat tattcgatta atagctccgg ttaagattca gattgccacc
    21181 gtgatgggaa aatacaatcc ttccgaaaaa accatggtcg attttgagtt agggttgagc
    21241 aacaatgacc tgaatttgta ttcgggtatc gatgatgcca ataatcaagg tgttgcgggt
    21301 aggtttaacg gcaaacaacg tttgtttaca aagcaatgga ccttggatgc ttttgccaat
    21361 taccaattta tccaacagga gttccgaacc atcgaacggc tttttaccat tgaatttgac
    21421 cgtgattgga acttgaccag taccacgggc aatcaaagtt tgttggtttc cgggttaaat
    21481 tttagtgctc ctgaaaacgg ctttgccaag tatcaatttg aaaagctgga tttctccgat
    21541 agttattcgg ggtcacggca tgtgtttgac gctcggtatt cttataaaaa atggctattt
    21601 caaaacaaca gcagtttctt gaaaagtgac ggctcctatt ccaccacttc gtttgcccgc
    21661 agtgatagtc gggtgaaata ccattggaac aaaaattggg caggcggtag ttttcggacg
    21721 gaagacaatg aagaaaaaat taaggctacc caatcacttt ctgccctcag tcaacggttt
    21781 tctgagtttg gcggtttcat tggacggggt gattcaacaa aagtatttgt agaagtgggt
    21841 tatttgcatc gggtgaatga cagtttgcaa aatggctttg tccaacgggt gaaccgttcc
    21901 cattcctact atttaaactc ccgtttgttg caaacagcga aaagtgattt atctgttttt
    21961 gtcaactacc gcaatttaca gtttgaggac gaaaccctgg atgattcacc gtcattaaat
    22021 tcccgcatct tgtataacga tcgatttttt aaccaattgc ttcaattgac tactgtttat
    22081 gaaacttctt cgggaacgtt agcccaacag gagtttacct atatagaagt agaccccgga
    22141 cagggcgtgt atacttggaa tgattataac aataatggca ttcaagagct ggccgagttt
    22201 gaagtggctc cttttcccga tcaggcaaaa tatgtgcggg tgtatttgcc caatcaggtg
    22261 tttgtgaata cacatcaaat taaattttca caagccttga ccttgaatcc cttgcaatgg
    22321 caaaaccaaa cgggttttaa aaagttcctt tcttattttt ataaccagac ttcgttttta
    22381 gtagatcgga aaacggaccg tgcttcgggg gaattcaatt tgaatccttt tcagcaggat
    22441 gaaactgatt tattggggct gaacaacagt ttcatgaaca atttgtccta taatcggggg
    22501 aaacaaaacc attctgtcac ctataccttt gtgaaaagta agtcgcagaa cctgctttcg
    22561 gtgggttttc aaaagacgat taatcaatcc catcagttgc aatataccca tttgttacaa
    22621 aagagttggc tgtttttagt caacgccaaa acgatcaatt cccagtcgga atcagaaaac
    22681 tacctcaacc gtgattttga gattttgggt tatcagtttg ctccccgcgt gagctattta
    22741 ttcactaaaa atgccagttt ggatttgttt tatgactacc aaaacaaaga aaatcagatg
    22801 ggagaattgg aaaccttaca acaaaatcgg tttggtactt cttttaccta tgccggtgaa
    22861 aaacaattta cgctgaatgg agaagtctct ttttacgcca atgattatgt gggggatgaa
    22921 ttatcggctg tgggttttca aatgttggaa ggattgcaac ccgggagaaa ccaaacttgg
    22981 cggttgttga ttcaaaagaa tttgacccgt tttttagacg tcaatgtgaa ttatcaaggg
    23041 cgaaagagtg aaaccagtga tacgattcac acgggcaatg tgcagttgcg ggcttatttt
    23101 tagggcaact tgttaaaata aagcccctct ttgattttta ttattatatt tgaaaataga
    23161 aattaatcaa cgctatgaaa tcaacctctt ttttattgct gctggcagga agtactttct
    23221 ttttaagttc ctgtaaaaat tcagaagaag ctcctgctga aacaccggct gtgacaacag
    23281 aagttcctgt tccggctgct gctgctgaag ccacgacccc tgagacaccc gctgccactg
    23341 ctactgaggc tactacggga gaagctccaa aattaaaccc ggctcacgga caaccttttc
    23401 accgttgtga tattgctgtt ggtgctcctt tagacggatc cggaccagca cctgcttccg
    23461 cggctccaac ccaggcggag gctccaaaat cattctttaa aacggtacag agcacacaac
    23521 cggcacctac tcctgctcct acgatgaatg cgactccgac accaactcca caaccgaaac
    23581 cggctcctac tgccacggct gatgccaaca cacctaagcc aaagaacaac ccggctcatg
    23641 gacaaccgca tcaccgctgt gatatcaaag tgggagaccc tttaccggat gcgtagaatt
    23701 aaatcgttta tctcaaattt tacaaaacaa tactggttca ggattcatta ataataaaaa
    23761 accgtaacta tcaattatat tgacaattac ggtttttaaa agtggagaag atggggctta
    23821 aatattccaa aaccccaaaa cctcaaaaac ctcaaatacc actcataaat ccaataaaat
    23881 caatacttaa caaagactta tcaaacattt atgtttgaat atgtttgtgt tttattgtat
    23941 gttttttagt aaatgtgggc taaatgtgga ctagattttg tatatttatg tattcaaaat
    24001 gacaaaatta tggcttcaat taagctcctt ttgcaaagca aaaacaatcc agcagtaatt
    24061 tacattcgtc tccgtgatgg tagaaataca gatgttaaag ctaaaaccaa ttatcatatt
    24121 gaccctgagt tatgggatag taaagaacaa agaccttcaa agaaagcttt aaaaaatatt
    24181 gactatgcaa acctggacac agacttgcaa gaaataaaaa ataacctact caaagaatac
    24241 aataaaagta agggtgtagt agccattgat ttgaattggc taaacgactt cataaaccca
    24301 cctcaacaag aagagaaact gccagaaaag ctaattgact atttagacaa gtatacagaa
    24361 tataaaaaac gagaagttaa agaaagtacc ttaaaaaaat gtggtgtcat taagcagctc
    24421 ttacttcgtt atgaagcaga cagaaaatta accctgtata tcaaaaacat caatttgcat
    24481 ttcaagaatg attttgaaaa ctattgcatt agtcaaggct atgctttgaa tacaattgca
    24541 cgtgctgtaa ggttcatcaa aacaatttgc aaccatgcaa aagcaaatgg tgtagaaact
    24601 catttccaac ttgacggtgt taaagccaaa tatatcaaag tggacaacat ttatctaaat
    24661 gaaaccgaaa tacagcaaat tgaaaatata gaatctgaaa agcttaccga aggattagaa
    24721 aatgaccgag attggctttt aataagttgt tattgcggac aaaggatttc tgatttttta
    24781 cgatttgata aatctatgat tcgatatgag aaaaacaaaa gtggtgttct aaaacccttg
    24841 attgaattta ctcaagtgaa aaccggaaaa atcatgacaa tacctttaca ccataaaatc
    24901 attgaaatag tgaataagta tggtggagaa tttccaagaa aaatatcaga ccaaaaatac
    24961 aatgataaca ttaaaatagt ttgtaagtta gcagaaatca aaaaacctac acatggtgtt
    25021 aaatttgatc ctgaaacaaa aaacaaagtt gaaaaagaat atgaaaagtg ggaattagta
    25081 tcttcacata ttgggagaag gtcttttgca agtaataatt acggaaaaat accaacctct
    25141 tttttaatgt acgttacagg gcatacaact gaagcaatgt tcttgaccta cattggcaaa
    25201 agcaataaag atatagcaat ggaattaaca aattattttt agtcgtatgt ctagttacag
    25261 taaaatatac agtggccaat ttaatgtcaa aaacattaat tacgcaaatg gtttaggatt
    25321 catttcgaaa aattttaaga gtttaaattg tattgatttt aataagcagt atgaaacatt
    25381 attacttctt tttctagaaa aggatttcac taacaatgaa atattgtttc taagaaacgg
    25441 gattctcgaa ttattttcag caaagctatt tttagataac cacacgccta aagacaagaa
    25501 ttatttcttt aatgaaattc ataaagagaa tgaaataaga tataaagacg acccttcaaa
    25561 agcaaatctt ttagaaaatc ctgattttat tgcgtatgca aaccaaagag cagctaaaag
    25621 tgttagttta gaaaatgaat attatgagat aagtaaagag ctaataaaaa ctgaattgct
    25681 catttttaaa accaaaattg aaagaatggg ttatgtagtt cattttaaaa acgatgatgt
    25741 tgaaatcatt gaaacttata aaaacgagaa gaaaaacaac aaaatacaac ttgataaaaa
    25801 agaaaaaaat acagccacta aaatcaataa cccctttata ctaaacgctt ctaaaagagg
    25861 taaaaaagta ttgaccaaaa ttctttttga aaaagtagcc ttaatagatg tgattgattt
    25921 tataaataaa aacacaactt tagaaaccaa agaattatta ataaatgaaa taaatggcac
    25981 ttataattta atgagaaatg aatttatgtt taaagtaaaa gatgaagata tatcaaaagc
    26041 tttaaatgta ttagaaaatt cgcttgaata tgtttatatg tataaaggag attatgaaag
    26101 atttaatttt tatgtgcgtg attttggaag tcataaaaca atatataatg tgtggggctt
    26161 accaactgaa tacaaagaag atactattaa attagtccac ctaaataatt taatgatcaa
    26221 ttaccacgat gatttttttg aaaaccaagt agaaggtttt gagcagatta aacacttagg
    26281 ctatatagta aataatttaa aagagatttt tattgataat aatgcagttt caaataatgt
    26341 tgattcaaaa aatagctcaa taggtattag taaagataaa gaatcaaaaa tggcagaaaa
    26401 atggtatgca ttactttatt ggcttgaact tttgtcgcaa aacaaaaaac ctccgactac
    26461 tttagaaggg tcatttgtta gaaaagaaat tgagaatatt ggtaaaaata aatccggaaa
    26521 atctggtcaa ggtttctatc gtgagttcac aagaatgagt gtagatatta aaaacttaaa
    26581 aagttttaat aattcatttg gtaaagattg gaaaaacata ataatagaat tatcaaacaa
    26641 tgatgaaaga attgtaaatt atttaaaaaa taattaatac ctcaggtatt acaaggtata
    26701 tacctgtaaa atacctctta aaatacctcg tttcatttgc cctgtataac tttaaaaaat
    26761 acaggaaatg caaacaaaat caattttact tgaaaaccta acagttgacc aacttcaaca
    26821 actaataggt acttcagttc gcaatggtat tttcgaactg caaaaacaaa ttcaaacaca
    26881 atccgacaac gaagccctaa tgactaggga tgaaacgtgc cagttcctca aagtggatag
    26941 ctcgactctt tgggcatgga cgaaccaggg aaaagtcaaa gcctacggca ttggtgcaag
    27001 acggtactac aaaaaaagcg aattactcga atgtctaacc ttactaaaaa aataagcgat
    27061 ggaatactac gaaaaaa
//
LOCUS       DAOTCW010000013        26817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_896_length_26817_cov_54.147411, whole genome shotgun sequence.
ACCESSION   DAOTCW010000013 DAOTCW010000000
VERSION     DAOTCW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 26817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26817
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_896_length_26817_cov_54.147411"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /gene="ssb"
                     /locus_tag="P5335_02015"
     CDS             <1..317
                     /gene="ssb"
                     /locus_tag="P5335_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRZ73686.1"
                     /translation="NKVQLIGHVGKDPIIKDFEGGKKLANLSLATKDVYLSKKGDQVE
                     QTQWHNIVAWGKTAEIIERFVEKGKQIGITGKLTSRSYDDKEGNKRYVTEVVVSELLL
                     MK"
     gene            408..728
                     /gene="ssb"
                     /locus_tag="P5335_02020"
     CDS             408..728
                     /gene="ssb"
                     /locus_tag="P5335_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRZ73687.1"
                     /translation="MKNRVQLIGHVGNDPEVKTFEGGKVANLNIATNDSYTNKKGEKV
                     EQTEWHRIVAWGPTAEIIEKLVNKGKEIAIEGKLSTRSYDDKEGNKRYITEVIVSDIM
                     LIGK"
     gene            754..1206
                     /locus_tag="P5335_02025"
     CDS             754..1206
                     /locus_tag="P5335_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRZ73688.1"
                     /translation="MKVKHFRVRVSGEHLQNDQDAINQFLTEVTVEKTATNFISGMID
                     YWSVLVFYTSKNAKPKESFESEKVAVLNYEELSQEEKNTLNSLKNWRTEKSSKLGLPQ
                     YMICHNSELMTIAKVKPETVEELKKIKGFGEQKISRYGTDIISLLTAS"
     gene            1303..1773
                     /locus_tag="P5335_02030"
     CDS             1303..1773
                     /locus_tag="P5335_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRZ73689.1"
                     /translation="MPVKSYQGESVGVQAPKAEEIHVEHYMNKSVVSFKTTQDILEIM
                     DALVKNCISGGPIVSDSNEVVGFISEGDCIRQLTESKYYNLPMEEMNIEHYMTKNVIC
                     VGPDESLLDIANKFLTTKKRNFPVVRDGKLIGLISQKNVLKAVLEWKGTHYRRH"
     gene            complement(1875..3281)
                     /locus_tag="P5335_02035"
     CDS             complement(1875..3281)
                     /locus_tag="P5335_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73690.1"
                     /translation="MKKTFLLLMLFCMYSILGQTKLVSVGLELKKSSDYHQVLPTVNS
                     LTKEVYVLAGDKEKSTLVKFNSALFFADSLQTKKVKNYPSAIANSFSEDGNPVYYWAS
                     EDFKQFMAVTYNFKQRTTNSVFFDIPLTNETVLTSFSKNNHFYILSIHDLSKKVKLYV
                     FEGNTFAEKIFDLEIYNPKELIETPFSLSNFLKTNPLEVVDNEVFNPLFIGCNKIKIY
                     PLNDKLLFTFDFNFSQTQILEIDWKTYEIIEKKFSQETLKSGVGKSNSFYHNGNLYQF
                     KANETQLIISKHDYSGKEPTLQYSVMQNEVISFKNSPLYMQSGNYQPQEIKNTQKFLN
                     RLKNKSLGLTVYKTQNDLLFTVGGTNEILSSGSLFFGTVATLGGIAVGVEIPSDFYSN
                     DITQSVFFECRFDSEFKPKQSQFDPLAEDFISGFLQENNSIITYNRIKYKDFYILSYY
                     DSKAKQLILRKFEDGYSF"
     gene            complement(3353..4042)
                     /locus_tag="P5335_02040"
     CDS             complement(3353..4042)
                     /locus_tag="P5335_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRZ73691.1"
                     /translation="MNLKVIAFDADDTLFVNEPYFQETERKFCSLMEDYLSHQSLSQE
                     LFRIEIQNLPVYGYGIKAYILSMIEAAMKISNNTISVEIVEKIIEYGKELLEKPIELL
                     DGVEETLSALHGRYKLVVATKGDLKDQHRKLHDSGLGKYFHHIEVMSDKQTIDYQKLI
                     GRLEIKPNEFYMIGNSLKSDVLPVLDMGGYAVHVPFHTTWEHEKISHKVEHPNFKTIE
                     KITEVLPFLIR"
     gene            complement(4053..6119)
                     /gene="metG"
                     /locus_tag="P5335_02045"
     CDS             complement(4053..6119)
                     /gene="metG"
                     /locus_tag="P5335_02045"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRZ73692.1"
                     /translation="MIENPKRYTITAALPYTNGPIHIGHLAGVYVPSDIYARYLRLQG
                     KDVAFICGSDEHGVAISMKAKKEGITPQQVIDKYDGIIRKSFIDFGISFDNYSRTSSK
                     IHHDTASEFFRTLYDKGVFIEEVTEQLYDEKADQFLADRFVMGTCPKCGNEEAYGDQC
                     EKCGSTLNATDLINPKSTITGTTPIKKETKHWFLPLDRYSDFLNDWILVGHRNDWKPN
                     VYGQVKSWIDGGLEPRAVTRDLDWGIDVPVEGAEGKKLYVWFDAPIGYISSTKEWATR
                     EGKDWKPYWKDADTKLVHFIGKDNIVFHCIIFPAMLKAEGSYILPDNVPANEFLNLEG
                     NKLSTSKNWAVWLHEYLIDFPEKQDVLRYALTANAPETKDNDFTWKDFQARNNNELAA
                     IYGNFINRVVVLTHKYYNGIVPQPNAFTEVDEQVLAELKAYPAVISSSIERYRFREAL
                     GELMNVARLGNKYLADEEPWKMVKTDPERVKTQMYVALQIATALGVLSEPFLPFTSKK
                     LVVDMLQCNKLTIGWELISKSTELIKPGHQIGQPEILFAQIDDAEIQKQIDKLEATKT
                     ANKLENKTIEPQKDTATFEDFSKLDLRVGTILEAEKMPKANKLLVLKVDTGIDIRTIV
                     SGIAEHFSPEEVVGKRVTVLVNLAPRALRGVESQGMILMTNNAEDKLVFVNPESDGVD
                     NGALIS"
     gene            6354..7250
                     /locus_tag="P5335_02050"
     CDS             6354..7250
                     /locus_tag="P5335_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRZ73693.1"
                     /translation="MITPPYLKNGDTIAIVATARKHIDDDLKTTKEFLENWGLKVIVG
                     SSIGLDQNQLAGTDEDRAKDFQQQLDNSNVKAIWCVRGGYGTVRMVDLLDFSKFKQNP
                     KWVIGFSDVTVLHSHLNTMGFQSIHGMMPVNIPRATPEAKESLRKALFGENLSYTIPF
                     DSMNKLGRVKGELVGGNLSILYSLFGSESAIDCADKILFLEDLDEYLYHIDRMMMNLK
                     RNGCLESLKGIVVGSMTKMKDNDIPWGKNALEIIEDITKTYSIPVVYNFPAGHIPDNR
                     ALIFGRQVTLEVSKKETRLIFE"
     gene            7385..7744
                     /locus_tag="P5335_02055"
     CDS             7385..7744
                     /locus_tag="P5335_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRZ73694.1"
                     /translation="MAKHNELGIKGELLAADFLQKNGYEILETNWVFQKAEIDIIAQK
                     DTILAIVEVKTRTSADFGLPQDFVKGKKIQNLVKAVDQYMIENDLDLEVRFDIIGIVL
                     NNHETRIEHLEDAFYYF"
     gene            7807..8466
                     /locus_tag="P5335_02060"
     CDS             7807..8466
                     /locus_tag="P5335_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRZ73695.1"
                     /translation="MKTISSVVEHYIKTKPFLLSALSQGIINLTSLSRNMMPELEQEL
                     GKDVKQGAVVMALKRLSEELDFRVNHKILKVLKNIGEITVRSSLIDFTFAVSETILDK
                     QAALIYEINKETGIFYTSSRGVNETNIVVSESVGHLVDKHFTGEKLIQKLDNLASITV
                     KLPKENISIPGIYYFIFQRLAWEGIIINEVISTSNEFTILVSEEEVDVAFKVIKDLKS
                     L"
     gene            complement(8524..>11670)
                     /gene="mfd"
                     /locus_tag="P5335_02065"
     CDS             complement(8524..>11670)
                     /gene="mfd"
                     /locus_tag="P5335_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRZ73696.1"
                     /translation="ENLINDQDVLFYPSSYRRPYQIEETDNANVLLRAEVLNRINSRK
                     KPSIIVTYPEALFEKVVTRKELDKNTLKVAVTDQISIDFINEVLFEYNFKRVDFVSEP
                     GEFSVRGGIIDVFSFSNDNPYRIEFFGNEVDSIRTFDVETQLSLEKQKKISIIPNVEN
                     KFLQENRESFLNYINPKTVLFVQETEMVLSQFDKLFDKATEAFGKLSNEIKQASPDEL
                     FINQSSFVKKALDFSMVEIGLKSIFKSEKTIEFHTKPQPSFNKQFDLLLNDLNENHFN
                     GIKNYLFCSNENQANRFHDIFESLDEENHENIRKQYKTIVFPLFQGFIDEENQLACYT
                     DHQIFERYHKFNIKNGYSKKQTITLKELTQLSVGDYVTHIDHGIGKFGGLQKIQVEGK
                     MQEAIKLVYADNDIVYVSIHSLHKISKYNGKDGTPPKIYKLGSNAWKVLKQKTKARVK
                     HIAFNLIQLYAKRRLEKGFACAPDSYLQKELESSFIYEDTPDQITATQDVKNDMERDR
                     PMDRLVCGDVGFGKTEVAIRAAFKAVDNGKQVAILVPTTILAYQHYRTFTERLKDMPV
                     TVNYLNRFRTAKQKSETLKGLSEGKVDIIIGTHQLVSKDVKFKELGLLIIDEEQKFGV
                     NVKDKLKTISATVDTLTLTATPIPRTLQFSLMAARDLSVITTPPPNRYPIESHVVGFS
                     EELIRDAISYEIQRNGQVFFINNRIENIKEVAGMIQRLVPDARVGIGHGQLDGKKLEE
                     LMLAFMNGEFDVLVATTIIESGLDVPNANTIFINNANNFGLSDLHQMRGRVGRSNKKA
                     FCYFITPPQSVMTEDARKRINALTQFSELGSGFNIAMKDLEIRGAGDLLGGEQSGFIN
                     EIGFDTYQKIMNEAIEELKENEFKELYHEENDMETKEFVKDLQIDTDFELLFPDDYIN
                     SVNERLNLYNELGNVKDEEGLLAYEQKLIDRFGPLPKEAQALLNSIRIKWIATRLGIE
                     KLIMKQGKMIGYFVGDQQSDYYQSSHFRKVLQFVQLNGNLCKMKEKETKSGLRLLLTF
                     ENVKSIRKALELMELMGR"
     assembly_gap    11671..11770
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11771..12041)
                     /locus_tag="P5335_02070"
     CDS             complement(<11771..12041)
                     /locus_tag="P5335_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73697.1"
                     /translation="MDLKNIYSVYNSSVKLVQLAESISIPEKKIQIKGLIGSSLSFVI
                     QSLFQKTELPFLLLFNDKEEAAYYLNDLENLINDQDVLFYPSSYRR"
     gene            complement(12042..12629)
                     /locus_tag="P5335_02075"
     CDS             complement(12042..12629)
                     /locus_tag="P5335_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73698.1"
                     /translation="MKIRVFFPFLIVFIFFSCQQDNEKRLIQHQKELVKNEVIFKKIS
                     SAWNFRTMNLQPSSQELVKDWAQWRLFLTELQQKPTSSIGAFQKKSKTLSKKTEEVLQ
                     SIPGQLLSPEFKSRFTVMLTKFRSLEMYLHLDAIPDQKIISLITDINLQLAAIELQMN
                     ELVRKNQIPTEQGEADMIRMLDTSRAVKNVPKNLN"
     gene            12772..13719
                     /locus_tag="P5335_02080"
     CDS             12772..13719
                     /locus_tag="P5335_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HRZ73699.1"
                     /translation="MSTKILIIGACGQIGTELTHQLRNLYGNENVIATDIRKLNNEVV
                     NDGIFEVLNALDFNQIEHIIEKYHVEEVYLMAALLSATAEKNPAFAWDLNMNSLFHVL
                     NLAKAKKIKKVFWPSSIAVFGPTTPRENTPQFTIMEPTTVYGISKQSGERWCEYYHNI
                     FGVDVRSVRYPGLISWSTPPGGGTTDYAVDIYHKALSDGKFECFLSEETRLPMMYMDD
                     AIRATIEIMQAPAEKIKIRSSYNLSGVSFTPKEIAAEIQKHIPNFTISYKPDFRQKIA
                     DSWPGSIDDSSAREDWNWKHEFNMDLMTVEMLENLKKSI"
     gene            complement(13723..14232)
                     /locus_tag="P5335_02085"
     CDS             complement(13723..14232)
                     /locus_tag="P5335_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRZ73700.1"
                     /translation="MKKIISLLFISISLISCSKSNPIENKEDIDKMLNLWHQAAAEAN
                     FENYFNLMTEDAVFIGTDATENWNRKDFENFSKPYFDKGKAWTFHPIQRNIYLDNKSK
                     FAWFDELLDSSFKICRGSGIVENVNGEWKVKHYVLSMTIPNDKSNQVVAIKDSLESAL
                     IMDLKQTKK"
     gene            complement(14379..15764)
                     /locus_tag="P5335_02090"
     CDS             complement(14379..15764)
                     /locus_tag="P5335_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73701.1"
                     /translation="MKDKKNLERLFQENFKDFEENPPEIVWKNIEEKLNEKEKKRRVI
                     PIWWKLSGVAALLVFGTLGIQYVMNSSGKEIKNEIVLDEMDLKKSETNPISPIENQKK
                     QGEKVDQIEKSKISVAIVNDNVENQKTVQNKSQNSTIHRKSKLPLSNSVNLIEEKYKL
                     AHNAKSNEIEKVEVNSISSTIEKQFVLTEYPSEQIKNNLDSTAISAVAENKVDPNALE
                     ELLKEKEQKPKIEPKLNRWQVSTAIAPVYLGSSSNGSPIDSEFANNPKEYNNTVSYGL
                     GINYDLNSKFSLRTGISNLSLSYNTNDVSFYPDINTKNLSTLNYSSQGATINVQDITA
                     TNGIQTVQNGAITQQMNYLEIPFELSYKVLDKKFGIQFISGFSTLFLNNNELMVVSEN
                     FSAELGKANNLNTVHFSTNVGVGFKYRFWRSFQASFEPTFKYQINTFSSNDGGFRPYI
                     IGLYSGVSYRF"
     gene            complement(15761..16303)
                     /locus_tag="P5335_02095"
     CDS             complement(15761..16303)
                     /locus_tag="P5335_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRZ73702.1"
                     /translation="MGLELLIQECKKKNTKAQEQLYRLFSSKLFNVCLKYSRNYAEAQ
                     DNLQDGFLLIFEKINQFEHKGSFEGWLKRVMINFILQKYRSHTVFEIVTENFPETIEV
                     EIEEENINMGYLLKIIQELPDRYRMVFNLYVLDGYSHNEIAEMMGISSGTSKSNLARA
                     KMILKDKIENQASNNMYEAK"
     gene            complement(16324..16731)
                     /locus_tag="P5335_02100"
     CDS             complement(16324..16731)
                     /locus_tag="P5335_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73703.1"
                     /translation="MKKFFWLIVIAVLFNSCSVDEEPKFHYEILAVDSYIVPETFIFN
                     ETHAIKVFYKKPTTCHAFQGFYFDSSGNERTVAVQSIISHTDNCLPLEDNLQEATFYF
                     TVKSYQTYLFKFYKGNDEEGNATYDIIEIPVTN"
     gene            complement(16825..17826)
                     /gene="recA"
                     /locus_tag="P5335_02105"
     CDS             complement(16825..17826)
                     /gene="recA"
                     /locus_tag="P5335_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRZ73704.1"
                     /translation="MSSEKEAKLKALQLTLDKLDKTYGKGTVMKMGDKAVEEVETISS
                     GSLGLDLALGVNGYPKGRIIEIYGPESSGKTTLTLHAIAEAQKAGGIAAFIDAEHAFD
                     RNYAQKLGVDIENLIISQPDNGEQALEIAENLIRSGAIDIVVIDSVAALTPKSEIEGE
                     MGDSKMGLHARLMSQALRKLTATISKTHCTVFFINQLREKIGVMFGNPETTTGGNALK
                     FYASIRLDIRRSSQIKDGENVVGNRTKVKVVKNKVAPPFRTAEFDIMYGEGISKTGEI
                     LDLAVEFEIIKKSGSWFSYGETKLGQGRDGVKALIKDNPELADELEIKIKDRIKETE"
     gene            17966..18700
                     /locus_tag="P5335_02110"
     CDS             17966..18700
                     /locus_tag="P5335_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRZ73705.1"
                     /translation="MHKLISYPISFLYYITFVLILSVFDPIQRICYYFFGYEAHKRSV
                     SILNFFLVRNTHLLGTTYDFQNREKLPNGVPLIIVANHQSLYDILPIIWFMRKWHPKF
                     VSKKELGKGIPSVSFNLKYGGSVLIDRKDPKQALPLIKKMGEYIEKHNRSTVIFPEGT
                     RSKTGVPKKFSESGLKILCKYAPSAYIVPISINNSWKMVRFGQFPLGLGNKITHTIHD
                     PFPIKGIPFEEIMLRTENAVVNGIKN"
     gene            18704..19693
                     /locus_tag="P5335_02115"
     CDS             18704..19693
                     /locus_tag="P5335_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HRZ73706.1"
                     /translation="MSIKNIRLEVMQLLEKNIDNFVNDYLIPVEKIWQPTDLLPDSEK
                     ETFFEEVKELREIAKDLPYDFWVVLVGDTITEEALPTYESWLIEVEGVDNEGRNGWSR
                     WVKNWTGEENRHGDVLNKYLYLSGRVNMREIEMTTQHLINDGFDIGTGRDPYKNFVYT
                     SFQELATYISHNRVAQMAKKYGDNKLSKMCKLIAGDEMRHHMAYSEFINQIFKVDPSE
                     MMLAFQYMMKQKVVMPAHFLRESGQKISSAFEHFSDSAQRIGVYTAHDYVDIMQKLIE
                     KWEIDKINGLTDEAEKARDFLMKLPARMARVSERLVVPQESHIFKWVQPALVK"
     gene            19703..20347
                     /locus_tag="P5335_02120"
     CDS             19703..20347
                     /locus_tag="P5335_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRZ73707.1"
                     /translation="MSIIDKTILFVKDKLQNAEGGHDWFHIERVYKNAISIAETETCD
                     LTVVKLGALLHDIADSKFHDGDEEIGPQKARNYLESQKVSEEIIKHVIQIIENISFKG
                     GNFEKQFTSKELEIVQDADRLDAMGAIGIARTFNYGGFKNRSIYNPTIAPKLNMSKEE
                     YKKSDAPTLNHFYEKLLLLKDKMNTETGKKLAQERHRFMELYLSQFYAEWEGEK"
     gene            complement(20356..21138)
                     /locus_tag="P5335_02125"
     CDS             complement(20356..21138)
                     /locus_tag="P5335_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRZ73708.1"
                     /translation="MNFENLLIEKDNGITVITINRPTKLNALNKATIDELHQAFDAEN
                     KDHSTKVIIITGSGEKAFVAGADISEFAHFSVEDGRKLAAKGQELLFDFVENLSTPVI
                     AAVNGFALGGGLELAMSAHFRIASDNAKMGLPEVTLGVIPGYGGTQRLPQLVGKGRAM
                     EMIMTATMIDAETAKNYGLVNHVVPQVELLTFAKSIASKITKNSSVAISNAIEAVNAN
                     FKDGINGYEVEIRNFGKSFGTEDFKEGTTAFLEKRKAEFPGK"
     gene            complement(21142..22575)
                     /locus_tag="P5335_02130"
     CDS             complement(21142..22575)
                     /locus_tag="P5335_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRZ73709.1"
                     /translation="MILLTVISSILIASVSIYQFRNEAKDYHRERLERKENAIKEHIN
                     YILNTTTYPLTTENIPLIFSERDKIHELSDIHGMEINFFDLEGKLLKSSKSSFAVDEE
                     PKSIDIYIVRLVQSSMDKRYVEVKEIDGVKNLSSFSEIKDTQFKPLAILNLPYIEDDG
                     YYERELKKFMIRFGQVYFFMLILSGLLAYFLSSYITKSLKTISDKLIKTQLTKNNEKI
                     ELEASSQEIELLIEAYNSMVDKLEESAVKLAQSEREQAWREMAKQVAHEIKNPLTPMR
                     LTVQSFQRKFDINDSNITQKLDDYSKTLLQQIDTMNSVASAFSNFASMPAQQNEQINL
                     INVVRLALEIFNENYIRFETNENEIFTNMDRTQLIRVITNLVKNATQSIQDNTINPSI
                     IVTVYKENSNAIITVKDNGIGISEENKPYVFEPKFTTKTSGMGLGLGIIKKIIENYNG
                     TITFESQPNVGTKFIVSFPIITNVKTI"
     gene            complement(22629..23171)
                     /locus_tag="P5335_02135"
     CDS             complement(22629..23171)
                     /locus_tag="P5335_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRZ73710.1"
                     /translation="MEYYNYIKSLHLIFVITWFAGLFYIVRLFVYHAEAKDKSEPEQG
                     ILIKQYQLMQYRLWYIITWPSAILASVFAFWLLLIMPQWLQQEWMIVKLGFVILLYLY
                     HWKCHLIFNQLQKNEVKNSSNFFRLWNEGATLILFAVVFLVILKSAINWIYGVVGIFV
                     FSILIMLGFKFYKKIRERNT"
     gene            complement(23158..24513)
                     /locus_tag="P5335_02140"
     CDS             complement(23158..24513)
                     /locus_tag="P5335_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRZ73711.1"
                     /translation="MAHSSGALGSEDIKQLLIKQAVPASIGILFMSINILVDTIFVGQ
                     WIGSLAIAAVTVVLPITFLISSLGMAIGIGGSSIISRALGAREKEKAMQTFGNQIMMT
                     LSLALLFVIVGFVYCDEILLLFGANGEIMKPAKEFFIPVLFGVPFLALCMMGNNVIRA
                     EGKAKFAMMAMIIPAFGNIFLDIIFIKFLNMGMFGAALATTISYFMCFVFILWFFIKK
                     SELKPGLHHFKFVSTIVREISSLGLVTFSRQGVVSVLAIIINHTLFTYGGEHSVVVYG
                     IISRMLMFALFPILGITHGFLPIAGYNYGAEKYNRVKETIAISIKYSALLATFIFLLI
                     LIFAKPIVSVFTTDVVVIEATPNTLRWVFAATPIIALQLIGAAYFQAAGKAIPALLLT
                     LSKQGFFLIPLVLILPNYFGIFGVWVAFPIADVLSTIVTAYFLRREMTTKLIEKTDGI
                     L"
     gene            complement(24514..25551)
                     /gene="hemH"
                     /locus_tag="P5335_02145"
     CDS             complement(24514..25551)
                     /gene="hemH"
                     /locus_tag="P5335_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRZ73712.1"
                     /translation="MKKGVLLVNLGSPESPTPKDVKPYLDEFLMDKYVIDVPFLLRAL
                     LVRGIILQTRPKKSAAAYAKIWWDEGSPLIILSKRMHKKVQANTSIPVSLAMRYGKPS
                     IESGLQELHDQGVTEVMLFPLYPQHAMASTVTILVLAEEIRKKKFPNMNFTILPAFYN
                     QKDYIRDLSNSIKKHLEGFDYDHLLFSYHGIPERHIRKTDITKSHCKIDGSCCNTPSP
                     AHEFCYRHQCYETTKQVVEFLGIPESKYSQTFQSRLAGDKWLTPYTDVEINKMPEKGI
                     KKLAVVTPAFVSDCLETLEEIAMEANHEFKEHGGEEFKAIPCLNDDDEWCKTLSRWID
                     QWAFAETQTNA"
     gene            complement(25568..26434)
                     /locus_tag="P5335_02150"
     CDS             complement(25568..26434)
                     /locus_tag="P5335_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRZ73713.1"
                     /translation="MGSQEEIKIENDFYLIRFQNDTNETQLFQRPVHTDLIQFHYGLK
                     GKAKFVFNQGNYALDLVEEKSLLLYNPQKELPVHLEIGPNSWMISVLISIKKLHTLFS
                     NEAEHIPFLSAENKEKKYYTEEDITPSMAIVLNQLFHYNLNSSIKNLYYKGKGYELLS
                     LYFNRSEDPNTEHCPFLIDEENIAKIKKAKEIVINNMAEPPGLQELADMIGLTLKKLK
                     TGFKQVYGDSVYSFLFDYKLEYARKLLDSGTYNVNEVGVLIGYSTSSHFISAFKKKFG
                     TTPKKYLMSLSN"
     gene            26652..>26817
                     /locus_tag="P5335_02155"
     CDS             26652..>26817
                     /locus_tag="P5335_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRZ73714.1"
                     /translation="MDHLKLSKHQTFYAVGLSYKKADAEVRGKFSLDDKAKANVLFQA
                     KQEGLDSLFII"
BASE COUNT         8798 a         4447 c         4386 g         9086 t
ORIGIN      
        1 aaaacaaagt tcagttaatc ggtcatgtag gcaaagaccc aatcatcaaa gattttgaag
       61 gaggaaaaaa attagccaat ttatcactcg caaccaaaga tgtctatttg agcaaaaaag
      121 gagatcaggt agaacaaacc caatggcaca atattgtagc ttgggggaaa acggcagaaa
      181 tcatcgaacg gtttgttgaa aaaggaaaac aaatcggcat caccggaaaa cttacttcca
      241 gatcttacga tgacaaagaa ggaaacaaac gctatgtaac agaagtagtt gtgagtgaac
      301 tgcttttgat gaaataactc atcaatttaa aaaaaacaat tttaattagt aaaagaatct
      361 gaaacgagtt cagatttaaa ctttaaattt tatacgccat gaacacaatg aaaaacagag
      421 tgcaattaat cggtcatgta ggaaatgacc cggaagtaaa aacctttgaa ggtggaaaag
      481 tagccaattt aaacattgct accaatgatt catacaccaa caaaaaagga gagaaagttg
      541 aacaaacgga atggcatcgt attgttgcct ggggtccaac agccgaaatt attgaaaaat
      601 tagtcaacaa aggaaaagaa attgctattg aaggtaagct ctctaccaga agttatgacg
      661 ataaagaggg aaacaaacgt tatatcaccg aagtcattgt tagtgatata atgctaattg
      721 gcaaataagt ttaaccacta aacacaagaa atcatgaaag taaaacactt tagagttcgt
      781 gtgagtggag aacacttgca aaacgaccaa gatgccatca atcaattttt aacagaagtt
      841 acggttgaaa aaacagcgac aaatttcata tcaggtatga ttgattattg gtcagtgttg
      901 gttttttata cttccaaaaa tgcaaaacca aaagaatcat ttgaaagtga aaaagtagcc
      961 gttttaaatt atgaggaatt aagtcaggaa gaaaaaaaca ccctgaattc acttaaaaat
     1021 tggcggacgg aaaaatcatc aaaattgggc ttgccacaat acatgatttg ccataattct
     1081 gaattaatga ctatcgcaaa agtaaaacct gaaaccgttg aagaattaaa aaaaataaaa
     1141 ggttttgggg agcaaaaaat ctctcgctac ggtacagata ttatttcatt attaacggcc
     1201 agttaattaa ttttatagaa atatggtagt ttaagaaatc atctgacttg tcggatgatt
     1261 tttttttgta tatttaaagt cctaaaagta aattgattag ctatgccagt aaaaagttat
     1321 cagggagaat ctgtaggagt acaagctccc aaagctgaag aaattcacgt agaacattac
     1381 atgaataagt cggtagtgag ttttaaaaca actcaggata ttttagaaat tatggatgcc
     1441 ctggttaaaa actgtatctc agggggacca atagtatcag attcaaatga agttgtaggg
     1501 tttatttcag aaggtgattg tattcgacaa ctcaccgaaa gtaaatatta taatcttcca
     1561 atggaagaaa tgaatattga acactacatg actaaaaatg ttatttgtgt tgggcctgat
     1621 gaaagtttac tcgacatcgc aaacaagttt ttgaccacaa aaaaaagaaa tttcccggta
     1681 gttcgcgacg gaaaactaat cggtttaatc agccaaaaaa atgttttaaa agcggtactt
     1741 gaatggaaag gaactcatta tagacgtcat taataaattg attattagga aatagaaatt
     1801 gtttttataa ataacaataa atcttttggc aaacaatatt tgcaaatgaa aaataggttg
     1861 ataatattca aaaattaaaa tgaataacca tcttcaaatt ttcgcaatat tagttgtttt
     1921 gcctttgaat cgtaataact taaaatatag aagtctttgt atttaattct attatatgtt
     1981 ataattgaat tgttttcttg taaaaatccc gagataaaat cttcagccaa agggtcaaat
     2041 tgagattgct tgggtttgaa ttcagaatca aatcggcatt caaaaaacac actttgagtt
     2101 atgtcattgg agtagaaatc tgatggtatt tcaacgccaa ctgcgattcc tccaagagtt
     2161 gctactgtcc caaaaaacaa actgcctgag cttaatattt catttgtgcc accaacagta
     2221 aataacaaat cgttttgggt tttataaacc gttaatccta aggatttgtt ttttaatcga
     2281 tttaaaaatt tttgtgtgtt tttaatttct tgaggttgat aatttccact ttgcatatat
     2341 aatggcgaat ttttaaatga aataacttcg ttttgcatta cactatactg gagtgtaggt
     2401 tcttttccac tataatcatg tttagaaatt atcaattggg tttcattggc tttaaattga
     2461 tataaatttc cgttatgata aaaagagttt gatttgccaa ctccactttt taaagtttct
     2521 tgggagaatt ttttttctat tatttcataa gttttccaat cgatttctaa aatttgagtt
     2581 tgagaaaaat tgaaatcaaa agtaaaaagt aatttatcgt ttaaaggata tatttttatt
     2641 ttattacaac caataaacaa aggattaaaa acttcgttat caacaacttc taaaggattt
     2701 gtttttaaaa aatttgaaag agaaaagggc gtttcaatta attcttttgg attataaatc
     2761 tcaagatcaa atattttttc tgcaaatgta ttgccttcaa aaacatacaa ttttactttt
     2821 ttactcaaat cgtgtatgct tagaatgtaa aaatgattgt ttttagaaaa agaggttagt
     2881 acggtttcat ttgttaatgg aatatcaaaa aaaacggaat tagttgttct ttgtttaaaa
     2941 ttataagtta cagccataaa ttgtttaaaa tcttctgagg cccaataata aacagggttt
     3001 ccatcctccg aaaaactatt tgcaattgct gaaggataat ttttaacttt cttcgtttgc
     3061 aaactatcag caaaaaacaa ggcactatta aattttacga gagtactttt ttctttatcc
     3121 ccggctagca cataaacttc ttttgttaat gagtttactg tcggtaaaac ttgatgataa
     3181 tcagaagatt tttttaattc taagcctaca gaaaccaatt tggtttggcc aagtatagaa
     3241 tacatgcaaa aaagcatcaa caaaagaaat gtctttttca tgtcttatta tttttgttat
     3301 accaaagata atttgttctt tgaagagaat tcaaaaataa atcaactatt ttctatcgaa
     3361 ttaaaaacgg taacacttct gtgatttttt caatcgtttt aaaatttgga tgttctactt
     3421 tatgactgat tttttcatgt tcccaagtag tgtgaaaagg cacatgaaca gcataaccac
     3481 ccatgtctaa aaccggcaat acatccgatt ttaaagaatt tccaatcata taaaattcat
     3541 tgggtttaat ttcaagacga ccaatgagtt tttgataatc aatggtttgt ttatctgaca
     3601 ttacttcgat atgatgaaaa tatttgccta atccggaatc atgcaacttg cgatgttggt
     3661 cttttaaatc acctttggtt gctactacta atttatatct tccgtgtaaa gccgataaag
     3721 tttcttctac accatctaat aattcaatag gtttttctaa tagttctttt ccgtattcga
     3781 taattttctc tacaatttca acagatattg tgttattcga aattttcatt gcagcctcaa
     3841 tcattgataa aatataggct tttattccat aaccgtaaac cggtaaattc tggatttcta
     3901 tcctaaataa ttcttgtgac aaactttgat gtgataaata atcttccatc aaactacaga
     3961 atttacgctc ggtttcctga aaatagggct cgttcacaaa taaagtgtcg tcggcgtcga
     4021 atgcgattac ttttaggttc ataaacaaat ttttaagaaa tcaaagcccc attatctact
     4081 ccatcactct ccggatttac aaaaactaat ttatcttccg cattattagt catcaatatc
     4141 atgccttggc tttcaacacc acgcaaggct cttggtgcta aattaactaa aacggttact
     4201 cgtttgccca caacttcttc cggtgaaaaa tgttcagcga ttcccgaaac tatggtgcga
     4261 atatcgattc cggtatccac ttttaaaact aataatttat tggctttggg cattttttca
     4321 gcttctaaaa tggttcctac acgaagatct agttttgaaa aatcttcaaa tgtcgcggta
     4381 tctttttgtg gttctatggt tttgttttcc aatttatttg cagtttttgt agcttccagt
     4441 ttgtctattt gtttttgaat ttcagcatcg tcaatttgtg caaataaaat ttcaggctgg
     4501 ccaatttggt gaccgggttt tattaattcc gtagatttgg aaatcaattc ccatccaatc
     4561 gttaatttgt tgcattgtaa catatcaaca accaattttt tggatgtaaa cggtaagaaa
     4621 ggctcagata aaacccctaa agcagtagcg atttgtaagg caacatacat ttgtgttttt
     4681 acccgttccg gatcagtttt taccattttc caaggttctt catcagctaa atatttattt
     4741 cctaaacgag ctacattcat caattcgccc aaagcttccc gaaaacggta gcgttcaatc
     4801 gaactggaaa taaccgcagg ataagctttt aattctgcta aaacctgttc atcaacttct
     4861 gtaaatgcat ttggttgtgg cacaattccg ttataatatt tgtgtgttaa aaccaccaca
     4921 cgattgatga aattaccata aatcgctgcc aattcattat tatttctcgc ttgaaagtct
     4981 ttccaagtaa aatcattgtc ttttgtttca ggagcattgg cggttaaggc ataacgcagc
     5041 acatcttgct tctctggaaa atcaattaaa tattcatgca accaaactgc ccaattttta
     5101 gaggttgata atttatttcc ttccaaattt aaaaactcat tggccggaac attgtcgggt
     5161 aaaatataac ttccttctgc ttttagcatc gctgggaata tgatgcaatg gaatacaata
     5221 ttgtcttttc caatgaaatg aactaatttg gtatcggcat ctttccaata gggtttccaa
     5281 tcttttcctt cacgtgttgc ccattctttg gtggatgaaa tgtatccaat cggagcatca
     5341 aaccatacgt ataatttttt gccctcagca ccttccaccg gaacgtcaat tccccaatct
     5401 aaatcgcggg ttacggcacg aggctctaaa ccaccatcaa tccatgattt tacttgaccg
     5461 taaacattcg gtttccaatc gtttctatgt ccaactaaaa tccaatcgtt tagaaaatcg
     5521 gaataacgat ccaatggtaa aaaccaatgt ttcgtttctt tttttatggg agttgttcct
     5581 gtgattgtcg atttcgggtt gattaagtct gttgcattta aagtagaacc acatttttca
     5641 cattgatcgc cataggcttc ttcgtttcca cattttggac acgtacccat gacaaaacga
     5701 tcggctaaaa actgatcagc cttttcatca tataattgct ctgttacttc ttcaataaaa
     5761 actcctttgt cataaagtgt tctgaaaaat tcagaagccg tatcatgatg gattttagag
     5821 gatgtccgtg aatagttatc aaacgaaata ccaaaatcga taaaagattt tcgaatgatt
     5881 ccgtcgtact tgtcaattac ttgttgtggt gtaatacctt cttttttggc tttcattgaa
     5941 atagccacac cgtgttcgtc gcttccacaa ataaatgcca catcttttcc ttgcaaacgt
     6001 aaatagcgtg cataaatatc agaaggaaca taaacaccgg ccaaatgtcc aatatgaatt
     6061 ggtccgttgg tgtaaggcaa tgccgctgta atggtatatc ttttgggatt ctctatcata
     6121 aaaatttgtt tctcgcccaa acacaaaatc gcgaaaaggc atgaattaat actattgttt
     6181 tgcaaaaata agcaaaccca tcgattctaa ttaatattta gagaatcgtt ttattgtttg
     6241 aggctttatt tgtttatttg ttagatggaa accgataaac tatgaaaaat tcgatgcttt
     6301 tttggtttgc tttgacagtt tttttcaatg ctaacgctca aatggacaaa aaaatgatca
     6361 caccacctta tttaaagaac ggcgacacga ttgctattgt ggcaactgcc cgaaaacata
     6421 ttgacgatga cttaaaaaca actaaagaat ttttagaaaa ttggggttta aaagttattg
     6481 ttggcagttc gattggatta gaccaaaatc aattggctgg aacggatgaa gaccgtgcaa
     6541 aagattttca acagcaattg gataattcga acgttaaagc catttggtgc gttcgtggtg
     6601 gatacggaac agttcgtatg gtcgatttac tagatttttc taagtttaaa caaaatccaa
     6661 aatgggttat tgggtttagt gatgtaaccg ttttacacag tcatctcaat acgatgggtt
     6721 ttcaatccat tcacggaatg atgcctgtga atattcccag agcgactccg gaagccaaag
     6781 aaagcttgcg aaaagccttg tttggtgaaa acctttcata taccattcct tttgattcta
     6841 tgaataaatt gggtagggtg aaaggcgagt tagtgggtgg aaatctttcg attttataca
     6901 gtttgtttgg ttcagaatcg gcaattgatt gtgcggataa gattttgttt cttgaagatt
     6961 tagatgaata tttataccat attgaccgca tgatgatgaa tctcaaacgc aacggttgtt
     7021 tggaaagctt gaaaggaatt gtggtgggaa gtatgaccaa aatgaaagac aatgatattc
     7081 cttggggaaa aaatgctttg gaaattattg aagatattac aaaaacctat tctattccgg
     7141 tagtttataa ttttccggca ggacatattc cggataatcg ggcgttaatt tttggacgac
     7201 aagtgacgtt ggaggttagt aaaaaggaaa ctagattgat ttttgaataa tattttttaa
     7261 gagaacacag atttataata tcaaagaatt agaaaaaatt aatgtttaaa ttatttaaat
     7321 tttagtaatt tttacaatct gtgttctata aaaacttttt tgaaaatttt atttaattta
     7381 ccaaatggca aaacacaatg aactgggcat aaaaggagaa cttttagctg ccgattttct
     7441 tcaaaaaaac ggctatgaaa ttctcgaaac gaactgggtt tttcaaaaag ccgagattga
     7501 tattattgct caaaaagata ccattttagc tattgttgaa gtaaaaacaa gaacttctgc
     7561 agactttggt ttacctcagg attttgtaaa aggaaaaaaa attcaaaact tagtgaaagc
     7621 agtcgatcaa tacatgattg aaaatgacct cgatttggaa gtacgtttcg atattatagg
     7681 tattgttctt aataatcatg aaacgagaat cgaacattta gaagatgcat tttattattt
     7741 ctaatgttat atttgtaaca aattgttttt atttatacat ttgtggcgtt tttaaaacat
     7801 tttcaaatga aaacaatttc ttcggtcgta gaacattaca tcaaaaccaa acctttttta
     7861 ttgagtgctt tgtcgcaagg catcatcaat ttaacttcgt tatcccgaaa tatgatgccc
     7921 gaattggagc aggaattagg caaagatgta aaacaaggag ctgttgtaat ggccttaaaa
     7981 cgtctttcgg aagaattgga ttttcgggta aatcataaaa ttctgaaagt attaaaaaac
     8041 atcggagaaa tcaccgttcg atcttcatta atcgatttta cattcgctgt ttcagaaaca
     8101 attttagaca aacaagccgc tttgatttat gaaatcaata aagaaacagg aattttctac
     8161 acgtcttcaa gaggcgtaaa cgaaaccaat attgttgtta gtgaaagcgt aggacacttg
     8221 gtagataaac attttacagg tgaaaaatta atccaaaaat tagataattt agcttctatt
     8281 actgtaaaac tacctaagga aaatatcagt attcccggaa tatattattt cattttccaa
     8341 cgtttagctt gggaaggaat tattattaac gaagttattt caacttccaa tgaatttact
     8401 attttggtaa gcgaagaaga agtagatgta gcttttaaag tgattaagga tttaaaaagc
     8461 ttataaaact tatatgctaa aaaattaaac atataagtca tataagttta atttttttga
     8521 aagttatctt cccatcaact ccatcaattc caacgctttc ctaatcgatt ttacattttc
     8581 aaacgttagc aacaaacgca aaccgctttt ggtttctttt tctttcattt tgcaaaggtt
     8641 tccattaagt tgaacaaatt gcaacacctt tctgaagtga gaagattgat aataatcact
     8701 ttgttgatcg cccacaaaat atccaatcat tttaccttgc ttcatgatga gtttttcaat
     8761 acctaatcgg gtggcaatcc attttatacg gatactgttg agcaaggctt gagcttcttt
     8821 cggtaaggga ccaaaacggt caattagttt ttgttcatag gctaacaggc cttcttcgtc
     8881 ttttacgttt cctagttcgt tgtataaatt taatcgttcg tttacgctgt tgatataatc
     8941 atccggaaac aacaattcaa aatcggtatc aatttgaaga tcttttacaa attctttagt
     9001 ttccatatca ttttcttcgt gatataattc tttgaattcg ttttctttta attcttcaat
     9061 ggcttcgttc atgatttttt gataggtgtc aaaaccgatt tcgttgataa aaccgctttg
     9121 ttctccacct aataaatctc cggcaccgcg aatttctaaa tctttcattg caatgttaaa
     9181 accgcttccc aattcactaa attgtgttaa cgcattaatt cgttttcgtg catcttccgt
     9241 catgactgat tgcggtggcg tgataaaata acaaaacgct tttttattgc ttcgacctac
     9301 gcgaccacgc atttgatgta aatcggacaa accaaaatta ttggcattat tgataaaaat
     9361 cgtgttggca tttggcacat ctaaaccgct ttcgataatg gtcgttgcaa ctaagacatc
     9421 aaattcacca ttcataaaag ccagcatcaa ttcttctaat tttttgccat ccaattgacc
     9481 gtgaccaatg ccaacacgag catcgggaac taatcgttga atcattcctg ccacttcttt
     9541 gatattttca atgcggttat taatgaaaaa cacttgacca tttcgttgaa tttcatacga
     9601 aatagcatcc cgaattaatt cttcactaaa accaacaaca tgactttcaa taggataacg
     9661 attcggaggt ggagttgtaa taaccgataa atccctcgca gccatcaacg aaaactgcaa
     9721 cgttctcgga atcggagtgg cggtcaatgt taacgtatcc accgtagcag aaatcgtttt
     9781 gagtttatct tttacattta caccaaattt ctgttcttcg tcaataatca acaagcctaa
     9841 ttctttgaac ttgacatctt tactcactaa ttgatgcgtt ccgatgatga tatctacttt
     9901 tccttccgac aaacctttta gtgtttcact tttttgtttg gcggttctaa atcggtttaa
     9961 ataatttacc gtcaccggca tatcttttaa tcgttcggta aatgttcgat aatgttgata
    10021 agccaaaatc gtagttggaa ccaaaatggc aacttgtttt ccgttgtcaa ccgctttaaa
    10081 agcggcacga atggcgactt ctgtttttcc gaaaccaaca tcgccacata ccaatcgatc
    10141 catcggacga tcgcgttcca tatcgttttt tacatcttgt gtagccgtaa tttggtcggg
    10201 cgtatcttca taaatgaatg agctttccaa ttctttttgc aaataactgt cgggagcaca
    10261 ggcaaaacct ttttccaaac gtcttttggc atacaattga atcaaattga acgcaatatg
    10321 tttgacacga gctttagttt tttgttttaa aactttccaa gcattcgaac ctaatttgta
    10381 tatttttggt ggtgttccgt cttttccgtt gtattttgaa attttgtgaa gcgaatgaat
    10441 gctcacatat acaatatcat tatcggcata aaccaatttg atggcttcct gcattttgcc
    10501 ttcgacttga attttttgca aacctccaaa ttttccaatt ccgtggtcaa tgtgtgtgac
    10561 ataatcgcct acagaaagtt gggttaattc cttaagcgta atagtttgct ttttagaata
    10621 accgtttttg atgttgaatt tatgataacg ttcaaaaatc tgatgatcgg tgtagcaagc
    10681 cagctgattt tcctcgtcaa taaatccttg aaaaagtgga aaaacaatgg ttttatattg
    10741 ttttcggatg ttttcgtgat tttcttcatc caaactttca aaaatatcat gaaagcgatt
    10801 ggcttgattt tcattggaac aaaacaaata atttttgatt ccgttaaagt gattttcgtt
    10861 caaatcattc aacaacaaat caaattgttt gttgaacgaa ggctgcggtt tggtatgaaa
    10921 ttcaattgtt ttttccgatt taaaaatcga tttcaatcct atttcaacca tcgaaaaatc
    10981 aagagccttt ttgacaaatg acgattgatt gataaaaagt tcatcgggtg aagcctgttt
    11041 aatttcatta gaaagtttgc caaaagcttc tgtcgcttta tcaaataatt tatcaaattg
    11101 actcaaaacc atttctgttt cctgaacaaa aagcacggtt tttggattga tgtaattgag
    11161 gaaactttct ctattctcct gtaaaaattt attttctacg ttgggaatga tggaaatttt
    11221 cttttgtttt tcaagtgaaa gttgtgtttc tacatcaaac gttctgatgc tatcgacttc
    11281 atttccgaag aattcaattc ggtacggatt atcatttgaa aacgaaaata catcgataat
    11341 tccaccacga accgaaaatt caccaggttc cgaaacaaaa tctacacgct tgaaattgta
    11401 ttcaaataaa acttcgttga taaaatcaat cgaaatttga tcagtaacag ccactttcaa
    11461 cgtgtttttg tctaattctt ttcgggtgac cactttttca aaaagagctt ccggataggt
    11521 aacaataatc gatggttttt tgcgggaatt gattcggttc aacacttctg cccgaagcaa
    11581 cacattggca ttatcagttt cttcaatttg ataaggtcgt cggtatgaac tcggataaaa
    11641 cagcacatct tggtcgttga tgaggttttc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn gtcgtcggta tgaactcgga taaaacagca catcttggtc gttgatgagg
    11821 ttttctaaat cattcaaata gtaagccgct tcttctttat cattaaaaag caacaaaaat
    11881 ggcaattctg ttttttgaaa taaagattga atgacaaacg acaacgaaga accaattaat
    11941 cctttaattt gaattttttt ctcgggaata gaaatgcttt ccgctaactg aaccagtttt
    12001 acggaagaat tataaacaga atagatgttt tttaaatcca attaatttaa atttttcgga
    12061 acatttttaa ccgcacggga tgtgtcaagc attcgaatca tatcggcttc accttgctct
    12121 gttgggattt gattttttcg aactaattca ttcatttgta attcaatagc cgctaattgt
    12181 aggttaatat ctgttataag gctgatgatc ttttggtcag gaatggcatc caaatgaaga
    12241 tacatttcta gcgaacgaaa cttggttaac attactgtaa atcgactttt aaattcagga
    12301 cttaataatt gaccgggaat tgattgtaaa acttcttctg tttttttaga aagggttttt
    12361 gatttttttt gaaaggctcc tattgaactg gttggttttt gttgtaattc agttaaaaat
    12421 aaacgccatt gagcccaatc ttttactaat tcttgggagg aaggttgaag attcattgtt
    12481 ctgaaattcc aagcactgct aattttttta aaaataactt cgtttttaac aagttctttt
    12541 tgatgttgaa tcaatcgttt ctcattatcc tgctgacagg aaaaaaatat aaaaacaatc
    12601 aaaaaaggga aaaacactcg aattttcatt atcagcaaat tagatttcaa aaatacaaag
    12661 gtaaattgaa tcttggttat tcgcgttaaa gctttccaaa aaataaaagt gatttcttaa
    12721 ctgatttctc aataacgaaa acgttatatt tgtactttaa atctacgact aatgagcaca
    12781 aaaattctga taattggagc ctgcggacaa ataggcaccg agttaacaca tcaattaaga
    12841 aacttatacg gcaatgaaaa tgtaattgct accgatattc gaaagttaaa taatgaagta
    12901 gttaatgatg gaatttttga agtattgaat gccttagatt ttaatcaaat tgaacacatt
    12961 attgaaaaat atcatgtaga ggaagtatat ttaatggctg ctttactctc tgctacagcc
    13021 gaaaaaaatc cggcatttgc atgggattta aatatgaatt cactttttca cgtattaaat
    13081 ttagcgaaag caaaaaaaat aaaaaaagtg ttttggcctt caagcattgc tgtttttgga
    13141 ccaactacac caagagaaaa cacgcctcaa tttaccatta tggagccaac aactgtttat
    13201 gggatttcaa aacaatccgg tgaacgatgg tgtgaatatt accataatat atttggtgtc
    13261 gatgttcgga gtgtacgtta tcccggattg atcagctggt caacgccacc cggtggagga
    13321 actactgatt atgcagtaga catttatcat aaagctttaa gtgatggaaa gttcgaatgt
    13381 tttttgtcgg aagaaacgcg tttgccaatg atgtatatgg atgatgcgat acgggcaaca
    13441 atagaaataa tgcaagctcc ggctgaaaaa attaaaattc gttcttctta taatttgtcc
    13501 ggagtgagtt ttacacccaa agagattgcg gctgaaattc aaaaacacat tccgaatttc
    13561 acaatttcat acaaaccgga ctttcgtcaa aagattgcag atagttggcc tggttcaatt
    13621 gatgattcat ctgctagaga agattggaat tggaaacatg aattcaacat ggatttaatg
    13681 accgttgaga tgttggaaaa cctgaaaaaa tcaatttaag aattactttt tagtttgttt
    13741 taaatccatt attagagcac tttcgaggga atcttttata gcaacgactt gattagattt
    13801 gtcatttggg atagtcatgg acaatacata atgtttaact ttccattctc cattaacatt
    13861 ttctacaatt cctgagcctc tgcaaatttt aaaggaagaa tctaatagtt cgtcaaacca
    13921 agcaaattta cttttattat ctagataaat atttctttgg ataggatgaa aagtccacgc
    13981 ttttccttta tcaaaataag gttttgagaa attttcaaag tcttttcgat tccagttttc
    14041 ggttgcgtct gttccaataa aaacagcatc ttctgtcatc aggttaaaat aattttcaaa
    14101 atttgcctct gctgctgcct gatgccaaag attgagcatt ttatcaatat cctctttgtt
    14161 ttcaattgga tttgattttg aacaagaaat tagacttatt gaaataaaaa gaagggatat
    14221 aatttttttc attggttttc aatttgaaat aaagatacaa aaaaagagca ctaaaaccaa
    14281 aagtgctctt ttctgagtta ttactgatgg caataaaagt aataacgaac ttaactaaag
    14341 tctaaaacaa ataagcaaaa ccaacttaag cttatcaatt aaaatcggta acttaccccg
    14401 gaatacaacc caataatata aggtctgaac ccaccatcat tgctgctaaa ggtgttgatt
    14461 tggtatttaa aggttggttc aaaacttgct tgaaatgatc tccaaaaacg gtatttaaaa
    14521 ccaacgccta catttgtact aaaatgaacg gtatttagat tatttgcttt tccaagttct
    14581 gcagaaaaat tttctgaaac aaccatgagt tcattgttgt taagaaaaag agtgctaaaa
    14641 ccagagataa attgaatccc aaatttttta tctaaaactt tataagaaag ttcaaaagga
    14701 atctccaaat agttcatttg ttgtgtaatc gcaccatttt gaacggtttg aattccgttt
    14761 gttgcggtaa tgtcttgcac gtttatggta gctccttggg aactgtaatt tagggtgctt
    14821 aaattttttg tattaatatc agggtaaaaa gacacatcgt tggtgttata acttaatgaa
    14881 agattgctga tacctgtacg taacgaaaat tttgaattta aatcataatt tatacctaat
    14941 ccataactca cggtattatt atattctttt ggattatttg caaattctga atcaattggt
    15001 gaaccgttgg aagatgaacc taaatacaca ggtgcaatgg cagttgaaac ttgccacctg
    15061 tttaatttag gttcaatctt tggtttttgt tctttttctt ttagcaattc ttccaacgca
    15121 ttagggtcaa ctttgttttc tgctacagca gaaatggcag ttgaatctaa attattttta
    15181 atttgttcag acggatattc tgtaagaaca aattgttttt ctatagtact tgatattgaa
    15241 ttaacttcta ctttttcaat ttcatttgat ttagcattgt gggctagttt atacttttct
    15301 tcaattaaat tcactgaatt tgaaagaggt aatttacttt ttctatgaat agtagaattt
    15361 tgagatttat tttgaactgt tttttgattt tctacattgt cattaacaat tgcaacacta
    15421 atttttgatt tttcaatttg atcaactttt tctccttgtt ttttctgatt ttcaatgggt
    15481 gaaatggggt ttgtttcaga cttttttaaa tccatttcgt ccaatacaat ttcattttta
    15541 atttctttac cggaagaatt cattacatat tgaatgccta aagttccaaa aactaacaat
    15601 gcggctacac cggataattt ccaccaaatc ggaattactc ttcttttttt ctctttttca
    15661 ttgagttttt cctcaatgtt tttccaaaca atttccggcg gattttcctc aaaatccttg
    15721 aaattttctt gaaataatcg ttctaaattt tttttatctt tcatttggct tcatacatat
    15781 tgttggacgc ctgattttct attttgtctt tcaatatcat tttagctctc gctaaattag
    15841 acttggaagt gccggaagat attcccatca tttctgctat ttcattatgt gaataaccat
    15901 ctaaaacata taggttaaac accattcggt acctatcagg caattcttga attattttta
    15961 acaaataacc catgtttata ttttcttctt caatttcaac ttcaattgtt tccggaaaat
    16021 tctccgtaac aatttcaaaa acagtatgag acctatattt ttgaagtata aaatttatca
    16081 ttactctttt taaccatcct tcaaaagaac ctttgtgttc aaactgattt attttttcaa
    16141 aaatcaataa aaaaccgtct tgtaaattat cttgagcttc tgcataattt ctcgaatact
    16201 tgagacatac gttaaacaat ttagatgaaa ataatctata taattgttct tgtgctttag
    16261 tatttttctt tttacattct tgtatgagta gttctaaacc caaaagttat tttctcttaa
    16321 aaattaattt gttacaggaa tttcaataat atcataagtt gcatttcctt cttcatcatt
    16381 gcctttataa aatttaaaca aatatgtttg ataacttttt actgtaaaat aaaaagtagc
    16441 ttcttgtaaa ttatcttcta atggcaaaca gttatcagta tgtgaaatta tactttgaac
    16501 agcaacagtt ctttcatttc ctgaggagtc aaaataaaat ccctgaaaag catgacatgt
    16561 tgttggtttt ttataaaaca cttttattgc atgtgtttca ttaaaaatga atgtttcagg
    16621 tacaatataa ctatctactg ctaaaatttc ataatgaaat tttggttctt catcaacact
    16681 acaggaatta aataaaacgg caataacaat tagccaaaaa aactttttca tttattcttt
    16741 ctaaattcta taatttacag atgcaaaatt gctcaaaagg ttgcgttgaa aataaaaaaa
    16801 cccgaataat tcgggtttta atttttattc agtttctttt attcggtctt taattttaat
    16861 ttcaagttcg tccgctaatt ccgggttgtc tttaattagg gctttaactc catcacgacc
    16921 ttgtcccaat tttgtttcac catagctaaa ccaagaacct gattttttta taatttcaaa
    16981 ttcaacggct aagtctaata tttcacctgt tttagaaatt ccttctccat acattatgtc
    17041 aaattcagca gttctgaaag gtggagcaac tttgttttta accactttta ctttggttct
    17101 gttaccaacc acattttcac cgtcttttat ttgagaagat cttctaatgt ctaaacgaat
    17161 ggaagcataa aattttaaag cattaccacc ggttgtagtt tcgggatttc cgaacatcac
    17221 gccaattttt tcacgtaatt ggttgataaa aaatacagta caatgtgttt tgctaatagt
    17281 tgccgttaat tttctcaacg cttgtgacat taaacgcgca tgcaatccca ttttagaatc
    17341 acccatttca ccttcaattt cactttttgg agttaatgct gcaaccgagt caatcactac
    17401 aatgtcaata gctcctgaac gaattaggtt ttcggcaatt tctaaagctt gttctccgtt
    17461 atccggttga gatatgatta aattttcgat gtcaacacct aatttttgag cataatttct
    17521 atcaaatgcg tgttctgcat caataaatgc agcaattcct ccagcttttt gagcttcagc
    17581 aatcgcgtga agtgtcaacg ttgtcttacc ggaagattcc ggaccatata tttcaataat
    17641 tcttcctttc ggataaccat tcacacctaa ggctaaatcg agacctaacg aaccggaaga
    17701 aattgtttct acttcttcaa cagctttgtc tcccattttc attacggtgc ctttaccgta
    17761 agttttgtct aatttgtcta acgttaattg cagggctttt aatttggctt ctttctctga
    17821 actcataata tttggcttta aatttatata caattgtttt tgtaaaaata cttttttttt
    17881 taatgtataa ataaattatt tttccacaaa agggaataac ctatgtaaat tcgattccgt
    17941 acttttgcct tcattacata ttgttatgca taaacttatt tcctatccaa tttcatttct
    18001 ttattatatc acgtttgtgt tgatattgtc tgtttttgat ccaattcaaa ggatatgtta
    18061 ttattttttt ggttacgaag ctcataaacg aagtgtttct attttaaatt tttttttagt
    18121 tcgaaacact catttgttag ggacaactta tgattttcaa aatcgtgaga aacttccaaa
    18181 tggagtgcct ttgattattg ttgctaacca ccaaagtttg tatgatattt tgccaattat
    18241 ttggtttatg cgaaaatggc accctaaatt tgtcagtaaa aaagaattag gaaaagggat
    18301 tcccagtgtt tcatttaatt tgaaatacgg agggtctgtt ttaattgaca gaaaagatcc
    18361 gaagcaagct cttccactga ttaagaaaat gggagagtac attgagaagc acaatcgttc
    18421 aacagtcatt tttcccgaag ggacaagaag taaaacaggt gtgcccaaaa agttttcaga
    18481 aagcggatta aagatattat gcaaatatgc accctcggct tatattgtgc caatttcaat
    18541 taataattca tggaaaatgg ttcgatttgg tcaatttcca ttgggattag gaaacaaaat
    18601 cacacacact attcatgatc ctttcccgat aaaaggaatt ccatttgaag aaataatgct
    18661 acgaacagaa aatgccgttg taaacggaat taaaaattaa aatatgtcta taaaaaacat
    18721 tcgattagaa gtaatgcaac ttttggaaaa gaacattgac aattttgtga atgattacct
    18781 tattcctgtt gaaaaaatat ggcaacctac cgatttatta cccgattctg aaaaagaaac
    18841 ttttttcgaa gaagttaaag aattacgtga aattgctaaa gatttaccat atgatttttg
    18901 ggttgtttta gttggtgata ctattacaga agaagcattg ccaacctatg aatcttggtt
    18961 aatagaagtt gaaggggttg ataatgaagg aagaaacggt tggtctcgct gggttaaaaa
    19021 ttggactggt gaagaaaatc gacatggtga tgtgttgaat aaatatttgt atttatccgg
    19081 aagagtgaat atgcgtgaaa ttgaaatgac cacccagcat ttaattaatg atggatttga
    19141 tattggaacc ggtagagatc catataagaa ctttgtgtac actagttttc aggaattagc
    19201 aacatatatt tcgcataacc gagttgcaca aatggccaaa aagtatggag ataacaagtt
    19261 atcaaaaatg tgcaaattga tagccggcga tgaaatgaga caccacatgg cctatagtga
    19321 attcatcaat caaattttta aagtagatcc aagtgaaatg atgttggcat ttcaatatat
    19381 gatgaaacaa aaagttgtga tgcctgccca ttttttacga gaatccggac agaaaataag
    19441 ttctgcattt gaacattttt cagactcagc tcagcgaatt ggtgtatata ccgcacatga
    19501 ttacgtagat attatgcaaa aacttattga aaaatgggaa atcgacaaaa tcaacggact
    19561 tactgatgaa gcggaaaagg ccagagattt tttaatgaaa ttacctgctc gaatggcaag
    19621 agtttcagaa cgattagtcg tgccacaaga aagtcacatt tttaagtggg tacaaccggc
    19681 tttagtaaaa taaaccaact ttatgtcaat aattgacaag acgattttat ttgtaaaaga
    19741 taaacttcaa aatgctgaag gaggccatga ttggtttcat attgaacgcg tttataaaaa
    19801 tgcaatttca attgctgaaa ccgaaacatg tgatttgacg gttgtaaaat taggggcttt
    19861 gttacacgat atcgccgatt caaaattcca tgatggcgat gaagaaatcg ggccccaaaa
    19921 agcccgtaat tatttagaaa gtcaaaaagt ttcagaagaa attattaagc atgtaattca
    19981 aattatcgaa aatatttctt ttaaaggagg aaattttgaa aaacaattta catcaaagga
    20041 acttgaaatt gttcaagatg cagaccgatt agatgctatg ggagccattg gaattgcaag
    20101 aacatttaac tatggaggat ttaagaatag gtcgatttat aatccaacta ttgctccgaa
    20161 attaaatatg agcaaagaag aatacaaaaa aagtgatgct ccaactttga atcattttta
    20221 cgaaaaatta ttgcttttaa aagacaaaat gaatacagaa accggaaaaa aattggctca
    20281 agaacgtcat cgttttatgg agttgtattt gagccagttt tatgcagaat gggaagggga
    20341 gaagtagttt tgtttttatt ttcctggaaa ctccgctttt cttttttcta aaaatgcggt
    20401 tgttccttct ttaaaatctt ctgttccaaa tgattttcca aagtttctga tttcaacttc
    20461 atatccatta attccgtcct tgaagttggc atttactgct tcaatggcat ttgaaattgc
    20521 cactgatgaa ttttttgtaa ttttagaagc gatacttttt gcaaaagtta gcaattccac
    20581 ttgtggaact acgtggttta ctaaaccata atttttggcc gtttcagcat caatcatcgt
    20641 tgcagtcata atcatctcca tcgctctgcc ttttccaact aattgaggta aacgttgcgt
    20701 tccgccatat cccggaatta cccctaaagt tacttcgggc aaacccattt tagcgttatc
    20761 ggaagcaatt cggaaatgag cagacatcgc taattctaaa cctccaccta aagcaaatcc
    20821 atttacggca gcaattaccg gagtagataa gttttccaca aaatcaaaca ataattcttg
    20881 tccttttgct gctaattttc ttccatcctc tacagaaaaa tgtgcaaatt cagaaatatc
    20941 tgctcccgcc acaaaggctt tttctccact tccggtgatg ataatcactt tggttgaatg
    21001 gtctttattt tcagcatcaa aagcttgatg aagttcgtct attgtagctt tgtttaaagc
    21061 atttaatttg gttggccggt taatggtaat tactgtaatt ccattgtctt tttctattaa
    21121 taggttttca aaattcatga tttaaatcgt tttaacgttg gtaataattg gaaaagaaac
    21181 aataaatttt gttcccacat ttggttggga ttcaaaagta atggttccgt tataattctc
    21241 tataattttt ttgataattc ctaggccaag tcccattcca ctggttttag tggtaaattt
    21301 tggttcaaaa acataaggtt tattttcttc agaaattcca ataccgttgt cttttacagt
    21361 aattatagca tttgaatttt ctttgtaaac cgttacaata atgcttggat ttatagtatt
    21421 atcttgaatg gactgggtgg catttttaac taaattggta ataacccgaa tgagttgagt
    21481 tctgtccata ttagtgaaaa tttcattctc attagtttca aatcgaatgt aattctcatt
    21541 aaaaatttca agagccaatc gaacaacatt aatcaaatta atctgttcat tttgctgtgc
    21601 cggcatagag gcaaaatttg aaaaagcact ggctacggag ttcattgtgt caatttgttg
    21661 caataatgtt ttgctgtagt catctaactt ttgagtaata tttgaatcat ttatatcaaa
    21721 ttttcgctga aaactttgca cagttaatcg catgggggtc aaagggtttt taatttcatg
    21781 agccacttgc tttgccattt ctcgccaagc ttgttcgcgt tcactttgtg ctaattttac
    21841 agcactttct tctaatttat ctaccatact attgtatgct tcaatcaaca attcaatttc
    21901 ttggctgctg gcttctagtt caattttttc attatttttt gtaagttgtg ttttgataag
    21961 tttatcagaa atagttttta gcgatttggt aatataactt gacaaaaaat aagctaataa
    22021 tcccgaaaga attaacataa agaaatatac ttgtccaaag cgtatcatga atttttttaa
    22081 ttcccgctca taatatccat catcctcaat atagggtaag tttaagattg ctaagggttt
    22141 aaactgtgtg tcttttattt cactgaagga ggaaaggttt tttacaccat caatttcttt
    22201 gacttccaca tatcgtttgt ccatactcga ttgtacaagt cttacaatat aaatatcgat
    22261 ggattttggt tcttcatcta cagcaaagga agatttagaa gattttaata actttccttc
    22321 tagatcaaag aaatttattt ccatcccgtg gatatcagaa agttcatgaa ttttatctct
    22381 ttcagaaaaa attaaaggaa tattttcggt agttaaggga taagttgtgg tgtttaagat
    22441 ataattaatg tgttctttaa tggcattttc ttttctttct agtcgttctc gatgataatc
    22501 ttttgcttca tttcgaaatt gatatatgga aacagaagct atcaaaatag aagaaataac
    22561 ggttaataga atcatcgaaa agaaaattct aacccgaaaa gagagtttgc ttattttttt
    22621 tgacagtatc acgtgtttcg ttcccgaatt tttttataaa atttaaaccc aagcataatc
    22681 agaattgaaa agacaaaaat tccgacgact ccgtaaatcc aattaatggc actttttaaa
    22741 ataaccaaaa aaacaacagc aaataaaatt aatgtggctc cttcattcca taatctgaaa
    22801 aaattggaac tgtttttaac ttcatttttt tgaagttgat taaaaataag gtgacatttc
    22861 caatggtata aatacaataa aatgacaaaa cctaatttga caatcatcca ttcttgttgc
    22921 aaccattgtg gcataatcag caacagccaa aaggcaaaaa cactagctaa aattgcacta
    22981 ggccaagtga taatatacca taatcggtat tgcataagtt ggtattgttt gattaggatt
    23041 ccttgttcag gttccgattt gtcttttgct tccgcatgat aaacgaataa acgaacaatg
    23101 taaaacaatc ccgcaaacca agtgatgaca aaaatgaggt gcaatgattt gatgtagtta
    23161 taatattcca tctgtttttt ctatcagttt agtagtcatt tctcttctca aaaagtaggc
    23221 cgttactatt gttgataata catcggcaat cggaaaggca acccatactc caaaaatacc
    23281 aaaataattt ggcaaaatta gcactagcgg aattagaaaa aaaccttgtt tgctcaacgt
    23341 tagtaataat gcagggattg ctttgccggc agcttgaaaa tacgcagctc caattaattg
    23401 taaagcaatt atgggtgtcg cggcaaatac ccatcgcaaa gtattggggg ttgcttcaat
    23461 cactaccaca tctgttgtaa aaacagaaac aataggtttg gcaaaaatta aaattaacag
    23521 aaaaataaag gtagccagta atgccgaata tttaatggaa atggctattg tttcttttac
    23581 ccgattatac ttttctgcac cataattata tccggcaatg ggaagaaaac cgtgggtaat
    23641 tcccaaaatc ggaaaaaggg caaacatcaa cattctacta ataattccat aaaccactac
    23701 ggaatgttcg ccaccatacg taaacaaagt atgattgatt ataattgcca aaacactcac
    23761 cacaccttgt ctggaaaagg taaccaaacc aagagacgaa atttctctaa caattgttga
    23821 aacaaattta aagtgatgta aaccgggttt taattcactt tttttaataa aaaaccaaag
    23881 aataaaaaca aaacacatga agtaagaaat ggttgtggcc aatgccgcac caaacatacc
    23941 catgtttaaa aattttatga aaataatgtc cagaaatata tttccaaaag ctggaataat
    24001 catagccatc atggcaaatt ttgcttttcc ttctgcccga atcacgttgt ttcccatcat
    24061 acataatgcc aaaaaaggaa caccaaataa aacaggaata aaaaattcct tagcaggttt
    24121 catgatttcg ccattcgctc caaaaagcaa tagaatttca tcacaataaa caaatcctac
    24181 aatgacaaac agcaaagcca aactcaaggt catcataatt tgattgccaa aagtttgcat
    24241 tgctttttct ttttctcggg ctcctaaggc tctggaaatg atagaacttc caccaatacc
    24301 aattgccatc ccaagtgaag aaattaaaaa ggtaataggt aaaacaactg taacagctgc
    24361 aatggctaag gaaccaatcc attgacctac aaaaatagta tccaccaaaa tattgataga
    24421 cataaaaagg attccaatag aagccggaac agcttgtttt atcaacaatt gtttgatgtc
    24481 ttccgagcct aatgcacctg aagaatgagc catttaagcg ttggtttgag tttcagcaaa
    24541 agcccattgg tcaatccatc gactaagggt tttacaccat tcatcgtcgt cattaaggca
    24601 agggattgct ttaaattctt cgcctccatg ctctttaaat tcatgattcg cttccattgc
    24661 aatttcttcc aaagtttcca aacaatcgga tacaaaagcc ggagtaacaa ccgctaattt
    24721 tttaatccct ttttcaggca ttttattgat ttcaacatcg gtataaggag ttaaccattt
    24781 gtcaccggct aaacgcgatt gaaaagtctg gctgtatttg ctttcgggaa ttcctaaaaa
    24841 ttcaactact tgttttgtgg tttcataaca ttgatggcgg taacaaaatt catgagcagg
    24901 tgaaggggtg ttgcaacacg aaccatcaat tttacaatga cttttggtaa tatctgtttt
    24961 gcgaatatgt cgctccggaa taccgtgata ggaaaacaat aagtgatcat aatcaaaccc
    25021 ttccaaatgc tttttgatag aattggataa atctcggata taatcttttt ggttataaaa
    25081 tgccggaaga atggtgaaat tcatgtttgg gaatttcttc ttgcgaattt cctcagctaa
    25141 aactagaatc gtaaccgttg aagccattgc atgttgcgga tacaaaggga aaagcatcac
    25201 ttcggtcacg ccttgatcat gcaattcctg caaacctgat tcaatgcttg gtttgccgta
    25261 tcgcatggct aaactcaccg gaatacttgt gttggcttgc acttttttgt gcattcgttt
    25321 ggataaaata atgagtggtg aaccctcgtc ccaccagatt ttagcataag cagctgccga
    25381 tttttttggt cgagtttgta aaataattcc acgcactaat aaagcacgta ataaaaatgg
    25441 cacatcgatt acgtatttat ccattaaaaa ttcgtctaaa tacggcttga catcttttgg
    25501 tgttggactt tcaggggaac ctaagttgac taataatact ccttttttca ttttttattg
    25561 tagcaattta atttgataat gacattaaat attttttcgg cgtagttcca aatttctttt
    25621 taaaagcgga gataaaatgc gatgatgtgc tgtaacctat taaaaccccc acttcattca
    25681 cattatatgt tccactatct aataattttc gggcatattc caatttgtaa tcaaataaaa
    25741 aactatacac gctgtcgcca tacacttgtt tgaaaccggt ttttaacttt tttaaagtta
    25801 aaccaatcat atctgctaat tcctgaagtc cgggaggttc agccatattg ttgatgacaa
    25861 tctctttcgc ttttttgatt ttggcgatat tttcttcatc gattaaaaaa ggacaatgtt
    25921 ctgtgtttgg atcttcactg cggttaaagt acaaacttaa caactcataa cctttacctt
    25981 tataatagag atttttgata gaactattaa gattgtaatg aaacaattga ttcaatacga
    26041 tggccattga tggtgtaata tcttcctcag tgtaatattt tttctccttg ttttcggcac
    26101 ttaaaaaagg aatgtgttcg gcttcgttag aaaataaagt gtgtaatttt ttaatggaaa
    26161 ttaataccga aatcatccat gagttcggac caatttctaa atgtaccgga agttcttttt
    26221 gaggattgta taataaaagc gatttttctt ctactaaatc caaggcataa ttaccctggt
    26281 tgaaaacaaa ttttgctttg ccttttaagc cataatgaaa ctgaatcaaa tcggtatgta
    26341 ccggacgttg aaaaagttga gtttcattag tatcattctg aaaacgaatt aaatagaaat
    26401 cattttcaat ttttatttct tcttgagaac ccatagcgat gttttttgtt ataaaaaaat
    26461 aaaataaacg tattgttatt tagaatgatt ctacataaaa gtcgttttaa gcccaataaa
    26521 tgctactatc ttagaatcat tataaacaat gacaaaaata attctttaaa cgatacaaaa
    26581 agaactttga gcgttatttt tttgaaaaga ataactataa ttttgtttaa ctttttagga
    26641 aagagtaaat tatggatcac ttaaaattgt cgaaacacca aactttttac gcggttggat
    26701 tgagttacaa gaaggcggat gctgaagtta gaggcaaatt cagtttagac gataaagcaa
    26761 aggcaaacgt tttatttcaa gcaaaacaag aaggattaga cagtttattt attatct
//
LOCUS       DAOTCW010000014        26793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_898_length_26793_cov_48.691376, whole genome shotgun sequence.
ACCESSION   DAOTCW010000014 DAOTCW010000000
VERSION     DAOTCW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 26793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26793
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_898_length_26793_cov_48.691376"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="P5335_02160"
     CDS             complement(<1..623)
                     /locus_tag="P5335_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRZ73715.1"
                     /translation="MKKSLMALSTLLMLGGTVSAQKVTFEEYTLDNGLHVILHQDNSA
                     PVVITSVMYHVGAKDENPERTGFAHFFEHLLFEGTQNIGRGEWFKIVTGNGGTNNANT
                     SDDRTYYYEIFPSNNLELGLWMESERLLHPVINQIGVDTQNEVVKEEKRLRVDNQPYG
                     NLIAEVKRNMFTNHPYRWATIGSMDHLDAATLKEFQDFNKKFYIPNNA"
     gene            825..1184
                     /gene="rplU"
                     /locus_tag="P5335_02165"
     CDS             825..1184
                     /gene="rplU"
                     /locus_tag="P5335_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRZ73716.1"
                     /translation="MYAIVEIAGQQFKVSKDQRVFVHRLASEEGSKVSFDKVMLLDED
                     GTITIGAPAIEGASVEAKVLKHLKGDKVIVFKKKRRKGYKKKNGHRQSFTQIVIEGIN
                     ASAPKKKAAKKAETTEE"
     gene            1208..1468
                     /gene="rpmA"
                     /locus_tag="P5335_02170"
     CDS             1208..1468
                     /gene="rpmA"
                     /locus_tag="P5335_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRZ73717.1"
                     /translation="MAHKKGVGSSKNGRESESKRLGVKIYGGQAAIAGNIIVRQRGSK
                     HNPGDNVYISKDHTLHARVDGVVKFQKKGDNKSFVSVIPFEA"
     assembly_gap    1521..1620
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1747..4059)
                     /locus_tag="P5335_02175"
     CDS             complement(1747..4059)
                     /locus_tag="P5335_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRZ73718.1"
                     /translation="MKNYLFLLTLSFFLIFPQLNFAQDKKPKVVLVLSGGGAKGVAHI
                     PLLQALDSLHIVPDLIVGNSMGSVMGGLYAMGYSGDSIAKMTKNMDWDKLLGGGVSFD
                     KVSVEEKSEFERYLIGLDIKEGKPTSVSSILSDQNLREYLSELTYPVYNITDFDKLSI
                     PFRALATDLVTGKEVVVDSGNLATAIRASMSLPAVFNPVPYKETLLVDGGVVNNFPTD
                     IAQQLGADIIIGSDVGGGMEPKEKLNNFVSVLVQTSMLTSNVKDPEKRKLCTILVDHL
                     PNLKYSTGDFAKSNEIYEEGKIATNQNMDALVALAEKLNKFKQRTHELPNVPKEFTLD
                     TIVFTNISKENLAFVKERMNIKPHTKYVSHDLVEGINRAMGTNLFSKITTSSFVNDGK
                     IGLEITFFENSKNQVNASLHYDTYRGVGVILNYTGRNILGESSRFLITADIAEQPRAR
                     IEYQDIFGSKKEWWWNTELYGENLIQELFVDNKLVTDLKYRSYQFNNQVNRNLNSFKT
                     YVGMSLNYQYTYLKPKADADLTEYPFKSYYFNNIELGVHFSYNDLDKVFFANKGTFFQ
                     AKISRSLLHDVELNFTDVTIPKISGATNGFTKLGLTFEKRIPLQRKLTGIIGATTNFI
                     FEDSLKSDEVSFSRHGYGAKYFLGGTSISPTSNRTVFPGLNEDELTVTQLMKMNVGLQ
                     TNLTGKIYLTPHIDIASVGFKTFNDYMDDAFSPKGKWQERTETSFLFSGGANISYLSL
                     LGPITFDASWVNDIDETNLYFSIGFLFNPS"
     gene            complement(4379..5695)
                     /gene="ahcY"
                     /locus_tag="P5335_02180"
     CDS             complement(4379..5695)
                     /gene="ahcY"
                     /locus_tag="P5335_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRZ73719.1"
                     /translation="MSTQTLPYVAFKVKDISLAAWGRKEIELAEAEMPGLMALREEYG
                     NSQPLKGARIAGCLHMTIQTAVLIETLIALGAEVTWSSCNIFSTQDQAAAAIAAAGIQ
                     VYAWKGLDEASFDWCIEQTLFFGEDRKPLNMILDDGGDLTNMVFDRYPELIPGIKGLS
                     EETTTGVHRLYERMKNGTLYMPAINVNDSVTKSKFDNKYGCKESAVDAVRRATDIMLA
                     GKRVIVCGYGDVGKGTAASFRGAGSIVTVTEIDPICALQAAMDGFEVKKLNTVVGNAD
                     IIITTTGNKDIVVGSHFEQMKDKTIVCNIGHFDNEIDMAWLNSNHGTSKIEIKPQVDK
                     YTINGKDIIILAEGRLVNLGCATGHPSFVMSNSFTNQTLAQIELWNNSANYKNEVYML
                     PKHLDEKVAALHLAKLGIELETLNEEQAAYIGVDVQGPYKPEYYRY"
     gene            5834..6472
                     /locus_tag="P5335_02185"
     CDS             5834..6472
                     /locus_tag="P5335_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRZ73720.1"
                     /translation="MPFHKKIHVDSKTVVLVWKISESYNDLFRSVSLKDNSLARMEGM
                     KSESHQKGFLAVRMLLQQAGYTDFDLYYDEFGKPHLKDGKHISISHSFDFSVIVLSDR
                     NIGIDLELRRDLVKKIARKFAAEKFVYESEHNSDDFIRKLTVIWGVKEAVFKVRNEIG
                     ISFKDHIFVCPFEMDDKQTTSLLDFNDTKQEFEVFFTEIESYTLVWLWETEK"
     gene            6469..7188
                     /locus_tag="P5335_02190"
     CDS             6469..7188
                     /locus_tag="P5335_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HRZ73721.1"
                     /translation="MKKIYQEIVQAKIDNRKLLAILLDPDKVDFDGLLLLTDKIKISL
                     ATHIFVGGSIVETNKIDLLIQFLKDNLTIPILLFPGNPSQISTYADGILFLSLLSGRN
                     PVFLIDHHVKAVPKLKNTSLEIIPTAYLLIESGSQTAVERVSQTEPLDRNNSNYVCQT
                     AMAGQFLGNKLIYLEAGSGAKLAVPLEMIHEVSKCISIPLIVGGGIRTLKGIKQAYDA
                     GADLVVIGTAFENDVTFFEND"
     gene            7181..7801
                     /gene="pnuC"
                     /locus_tag="P5335_02195"
     CDS             7181..7801
                     /gene="pnuC"
                     /locus_tag="P5335_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRZ73722.1"
                     /translation="MIDFLFSQYKTYSSFHIYLEIVAVLFGLVSVWYAKKDNILVFPT
                     GIISTTIFIYLLWKWSLLGDMSINGYYTIMSIYGWYHWTRKKNNQTVFPIAKISENEK
                     KWAIIIFIVSSIGVILIYQFFDKFTQWTAYVDTLTTGLFFVGMWLMAKRKIEHWIFWI
                     IGDIISIPLYFCKGYTFTSLQYLIFTIIAFYGYIEWKKILNSNTQA"
     gene            7765..9879
                     /locus_tag="P5335_02200"
     CDS             7765..9879
                     /locus_tag="P5335_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HRZ73723.1"
                     /translation="MEENLKQQHTSIIKIAIYGPESTGKTTLAKQLAEHFNTVWIPEF
                     ARDYLQDKWNKTKQICQQEDLLPIAIGQTKLENEGLQKANQVLFSDTNLMVTKVFSDI
                     YYGNTNPILQKAAKKHKYDLFLLTDIDVPWEKDDLRDRPDNREDTFKLFEDALIQYNK
                     PFIKISGSKEKRFQKATLLINDFLEAKGRGFNSFDFIQFYQRNVELDKVKNQIHFLTE
                     GIPRIDLDRIAKIDDGIEQLSKEEAIYYANFFDNKKNQLKLKKFVPASGAASRMFKFL
                     NDFLHDFKLGEESINAYINRKKAKNLSLFLIGIEKLPFYEKVLSRVKIIFPEYESWGY
                     DSKMFAFIKIMLAKNEFDFANKPKGVLPFHKYKSHCATPIEEHLNEAVYYAASNQKAH
                     LHFTVSEEHQEDFEKIVESVKPKIENQFQTEINVTYSYQNKATDSIALDMENNLFRDD
                     HDLLVFRPGGHGALIENLNRLDADIIFIKNIDNVIQNHIEEIALYKKALAGILLDKQQ
                     QIFLYLSQLDTDKVDKVFLDDVVTFIQTKLHFEVVEDFSKYTKESKIDYLKNYLNRPI
                     RVCGMVKNEGEPGGGPFWVRDKKGLVFLQIVETTQIDETDATQVKILESSTHFNPVDL
                     VCGIRNYKGVNFDLNEFVDHNSGFVVHKNKGGKELKAYELPGLWNGAMAKWLTVFVEV
                     PLITFNPVKTVNDLLKTAHQPQ"
     gene            9879..10280
                     /gene="arfB"
                     /locus_tag="P5335_02205"
     CDS             9879..10280
                     /gene="arfB"
                     /locus_tag="P5335_02205"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRZ73724.1"
                     /translation="MDSDKIIAELNFKAVRSSGAGGQNVNKVSSKVVLSFDLFNSQCL
                     SDDEKTVLFEKLKTKLTVEGILILNCDENRSQLKNKEIVTKRFLKLIQNALIIPKKRK
                     LTKIPRSVIEKRIKAKRNTSELKQNRKRPEA"
     regulatory      10357..10452
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10539..12755
                     /locus_tag="P5335_02210"
     CDS             10539..12755
                     /locus_tag="P5335_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ73725.1"
                     /translation="MKTLFNKFCLKGTKSSGRCCFLSLLLFSFSGFSQETVYDTTKTK
                     DISLDEVLVSAIRVTAQSPVTFSNLRKEEFQSRNLGQDIPLMMNYLPSVVTTTDAGAG
                     VGYTGIRVRGSDATRVNVTINGIPYNDSESQGTFWVNMPDFASSTESLQLQRGVGTST
                     NGAGAFGASLNVLTDNYSKESKGEISNSFGSFNTRKHTVKFSTGLMNEQFEIAGRLSA
                     INSDGYIDRASSDLKSYFLQGTYVGNSTLIKGLVFGGIQKTYQAWYGLEDPEKLENDR
                     TFNVAGMYYDENGKMKFYDNETDNYQQDHYQLHWNERWSEHWSSNFALHYTKGKGYFE
                     QYKENEDFSDYGLEPIVLGGETIDMTDLIRRRWLDNDFYGTTFSVNYNKDKVDVIIGG
                     GANKYEGAHFGEIIWAQYASQSEIRDRYYNDFSSKTDVNVFAKVNYQLTTKWRLFGDM
                     QYRNVGYQANGAETGLVNDKFNFFNPKAGVTFVLNEKQNFYFSYSKANREPNRNDYEN
                     GNPKPEKLDDYELGWRFSTEKAKLNVNGYFMKYKDQLVLTGELNDVGAPVRANSGDSY
                     RLGLEVDATFKLTNKWFLQPNVTLSQNKNQDFVFQRDGVFKDLGTTNIAFSPDLIAAN
                     RLVFMPIQNFQVSLLSKFVGEQYMGNIDAESSKLDSYFVNDLNVSYEIVTKKVFKSIR
                     IDVLINNIFNLEYESNGYFYTYDDDFSDPGTVTTIEGAGFYPQAGINLLAGLTLLF"
     gene            complement(12798..13217)
                     /locus_tag="P5335_02215"
     CDS             complement(12798..13217)
                     /locus_tag="P5335_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73726.1"
                     /translation="MKKIILVLFSTLFLFGCDSGGGNDNNPYIPNYTFTVDINVNLPS
                     YSNLQFPSNAVYIPQGTAGARGVIVFNSGSGYLAYDAACPNQPISGCSTMLISGINAI
                     CPCDDEQYSLFTGLSSLQYPMKRYRVEVNLPNIRVYN"
     gene            13452..13928
                     /gene="greA"
                     /locus_tag="P5335_02220"
     CDS             13452..13928
                     /gene="greA"
                     /locus_tag="P5335_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRZ73727.1"
                     /translation="MSTVSYYTAEGLKKLKEELETLKHIERPKASQAIAEARDKGDLS
                     ENAEYDAAKEAQGLLELRISKMEEVYSNARLIDESQLDMSKALVLSNVKIKNQANGME
                     MKYTLVAESEADLKSGKISVTSPIGKGLLGKSVGDVAEITVPNGVLKFEILDISRD"
     gene            13937..14326
                     /locus_tag="P5335_02225"
     CDS             13937..14326
                     /locus_tag="P5335_02225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRZ73728.1"
                     /translation="MASIFTKIINGEIPCYKIAEDDNFIAFLDVNPNAKGHTLCVPKQ
                     EINKLFEMEEDHYLNLMKFSRTIAVALEKAISCKRIGMSVIGLEVPHVHVHLIPLNEM
                     DEVRFQKKVSFTKEEFEEIAKNIKANL"
     gene            complement(14359..15513)
                     /locus_tag="P5335_02230"
     CDS             complement(14359..15513)
                     /locus_tag="P5335_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRZ73729.1"
                     /translation="MQFSESRNVTRWILILTSFVIVSLILWNTYTFFQRFKKDERLKM
                     ELWAEAQTNVFSSDVDADISLPLKIISNTTTIPIIVTDENDSIVNKTNIEDIDEKDSA
                     KLRSLLEKLKKENDPIVMEYVEGKFRYLYYGDSSLLNKLKYYPLALLLIIFLFGLLVF
                     NFYQTSKIATQNKLWAGMAKETAHQIGTPLSSLIGWLEILKAENVAESTLLEIQKDIT
                     RLQTITDRFSKVGSEPVLEKLDIIAETEQSYDYLKSRFSKQVEFTFLAPPHPIFVSIN
                     PALHSWTIENLVKNAIDAMKGRGKLDLNIIEEEKEVRIYVSDSGKGIPKNQFKKVFDP
                     GFTTKKRGWGLGLSLTKRIVEEYHKGKIKVHHSEIDKGTTMMLSFKKTTS"
     gene            15617..16495
                     /locus_tag="P5335_02235"
     CDS             15617..16495
                     /locus_tag="P5335_02235"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRZ73730.1"
                     /translation="MVSISPKDISPLKLQGYLQSAVGPRPIAFASTLDKNGNPNLSPF
                     SFFNVFSSNPPVLIFSPARRVRDNTIKHTLINCEATREVVINVVNYEIVQQMSLSSTE
                     YADGVNEFLKSGLTMLPSEVVKPFRVAESPVQFECKVNEIISLGTEGGAGNLIICEVV
                     KLHIDESILDENGNIDQHKIDLVSRMGANWYSRSNEGLFEIPKPLATLGIGVDQIPDF
                     VKESPVFNGNDLGMLGNVESLPTNEEINIFVRDNIQVKGVLSSADEMKVHEKAKEFLN
                     KQDAVSAWKVLLAKRV"
     gene            16531..16905
                     /locus_tag="P5335_02240"
     CDS             16531..16905
                     /locus_tag="P5335_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HRZ73731.1"
                     /translation="MEVTGKIRVVNPEQQVSATFRKRELVVSTEEQYPQHISINFVQD
                     KCDLLNSYKVGEAVKVSINLRGREWVNPQGETKYFNDIQGWRIERLQAEAPAAGMPPM
                     PAAEAFEPATNFNEEEHDDLPF"
     gene            17021..17656
                     /gene="aat"
                     /locus_tag="P5335_02245"
     CDS             17021..17656
                     /gene="aat"
                     /locus_tag="P5335_02245"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRZ73732.1"
                     /translation="MYFLSTELYFPPVTEASSEGILAVGGDLSIERLLLAYHSGIFPW
                     FDNDEPILWWSPSERMVVNPQDYKIAKSLRNILNRNIFKVTFNQNFTEVIKKCQKIKR
                     KGQRGTWITDSMLEAYIKLHETGFAKSVEVWQDNELVGGLYGVDLGKVFCGESMFSTV
                     SNASKVAFVSLIQKLKEENYHLLDCQVQNDHLEKLGAFEISREVYMKVLKG"
     gene            complement(17666..18313)
                     /locus_tag="P5335_02250"
     CDS             complement(17666..18313)
                     /locus_tag="P5335_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73733.1"
                     /translation="MFKKFASLTLFTFLLISFLNCGKNEKKTDSSYEKIQTVEKDNNS
                     SLIIEEKVENSTTQKIDSKITKKEPVIAATEPIPTLRKTESKSVAIPKNHKIYTNLSA
                     ILNTIQVGQTVTKEELIKQNKLPKDASSIVKSVKKTAENELYIEWKSTWLVEKVSDVK
                     LNDDRIKVEFKDNIVYTSGPAIGIKYDGKIYTDLIIKGDRAYIPSVKEYSWRIGK"
     gene            complement(18434..18994)
                     /locus_tag="P5335_02255"
     CDS             complement(18434..18994)
                     /locus_tag="P5335_02255"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRZ73734.1"
                     /translation="MEIKNRCAWCEKDDLYRTYHDEEWGNPVYDDQKLFEFLFLETFQ
                     AGLSWYTVLAKRENFRIAFENFEYEKVALFTENHIQELMQNTGIIRNQLKIRAAVSNA
                     NAYIKVIEEFGTFATYIWNFTNGKPLVNQFKELKDIPASTPLSDAISKDLKKRGFKFV
                     GSKVVYAHMQATGMVNDHLLSCWKRK"
     gene            19154..20242
                     /gene="ald"
                     /locus_tag="P5335_02260"
     CDS             19154..20242
                     /gene="ald"
                     /locus_tag="P5335_02260"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRZ73735.1"
                     /translation="MKIGVPKEIKNNESRVGMTPAGVFELIKNNHTVYVQQDAGFGSG
                     FFDTDYAVVGAILVPTAAEVYAQSDMIVKVKEPIPEEYPLIKENHVVFTYFHFASSEE
                     LTIAMINSKAVCIAYETVEETDGSLPLLTPMSEVAGRMAIQQGAKYLEKPIKGRGVLL
                     GGVPGVPPGKVLVLGAGIVGIQAAKMAAGLGAHVTILDINMKRLRYVNDVMPSHVVTE
                     FSSEYNIRRHIKNHDLIIGAVLIPGAKAPKLITRDMLKEMHPGTVMVDVAVDQGGCFE
                     TTVATTHENPTYIIDDVVHYCVANMPGAVPYTSTLALTNVTLPYVLKLANLGWEMATE
                     RYPDLRKGLNIIHGEIIYKELADTFNFS"
     gene            complement(20319..20537)
                     /locus_tag="P5335_02265"
     CDS             complement(20319..20537)
                     /locus_tag="P5335_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HRZ73736.1"
                     /translation="MQNTDPLHGKTLQAIVEYLVDYYGFEMLGTLIKIKCFTENPSIK
                     SSLTFLRKTDWARKKVEELYIDTITKKK"
     gene            complement(20630..21592)
                     /gene="tsf"
                     /locus_tag="P5335_02270"
     CDS             complement(20630..21592)
                     /gene="tsf"
                     /locus_tag="P5335_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRZ73737.1"
                     /translation="MATITAADVNKLRQTTGAGMMDCKKALVEAEGDFDKAIQNLREK
                     GQKVAANRSDRESSEGAAVSFVNADKTKGAIITLNCETDFVGKNESFVTLAKQLVERA
                     INFSSKEEFLASDFNGITIAEKLIEQTGVIGEKIEIGGFEILEGAFVGSYVHVNKIAA
                     LTAISEPISNGDVLTKDISMQVASMGADTLSYKDFDPAFVASELAARIAIIEKENEEA
                     KRLGKTLKNVPKYISFSQLTEEVIKQAEEDAKAELKAEGKPEQIWDKIIPGKIQRFIS
                     DNTTLDQEKALLDQNFIKDDSKKVSDYVKGFNVEITGFKRVALG"
     gene            complement(21739..22539)
                     /gene="rpsB"
                     /locus_tag="P5335_02275"
     CDS             complement(21739..22539)
                     /gene="rpsB"
                     /locus_tag="P5335_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRZ73738.1"
                     /translation="MANKVEVKDLLEAGVHFGHMTRKWDPNMAPYIYMERNGIHIINL
                     YKTAAKIEEANEALKKIAASGRKVLFVATKKQAKDIVAEKAAAANMPYITERWPGGML
                     TNFVTIRKAVKKMASIDRMKKDGTFNTLSKKERLQVDRLRAKLEKNLGSIADMSRLPA
                     ALFVVDIKAEHIAVKEAQKLNIPVFAMVDTNSDPRQVEYVIPANDDASKSIDKILSLV
                     TGAIIEGLSERGSEKDGDAVVEDTVEEVVAVEAAPAVEAEEAKTTSEE"
     gene            complement(22684..23070)
                     /gene="rpsI"
                     /locus_tag="P5335_02280"
     CDS             complement(22684..23070)
                     /gene="rpsI"
                     /locus_tag="P5335_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRZ73739.1"
                     /translation="MGVIHKIGRRKCAVARVYVSEGTGKITVNKREFENYFPTATLQY
                     KVMQPMSMTENATNFDVKVNVQGGGSTGQAEAVRMAIARAMCEVQAENRAILKPEGLL
                     TRDPRMVERKKFGQKKARKRFQFSKR"
     gene            complement(23070..23525)
                     /gene="rplM"
                     /locus_tag="P5335_02285"
     CDS             complement(23070..23525)
                     /gene="rplM"
                     /locus_tag="P5335_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRZ73740.1"
                     /translation="MNTLSYKTVSANKATADKQWIVVDAEGHNLGRFASKVAMLLRGK
                     YKPNYTPHVDCGDNVIVINAEKINLTGNKLDDKTYIRHTGYPGGQRSLTAKVMQQKNP
                     ALLVEKAVKGMLPKNKLGAELFRNLNVNVGIEHKHGAQQPKTVNLNDLK"
     gene            23828..24850
                     /locus_tag="P5335_02290"
     CDS             23828..24850
                     /locus_tag="P5335_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRZ73741.1"
                     /translation="MKPKATLKQISKDLGISVSTVSKALSDSHEISEPTKIRVQEYAK
                     LQNYKPNTMAKNLKNQRTNTIGVIIPNILNPFFAKVFSGIEKAANAKGYNVITYISNE
                     SHAKEKQAMDLLSNGTVDGFILSVAQETQSLHETQHFKDILREGMPIVMFDRTMEDVS
                     CDKVIVDDFESSVEAVNHLMKLGCNKIALLSCIDNLSVGKHRALGYERALQDHKRSVE
                     DYLVIKTDSEEDFDARLKKLLSSGEVDGILAVDEHASTMAMKVAIQKGIKIPEELCII
                     GFADGVWSRRLTPSLSTISQHGPEIGEEAAKLLIEKLERKQEIPTFKTTVVKTELRQR
                     DSTRRV"
     gene            complement(24953..>26793)
                     /gene="polA"
                     /locus_tag="P5335_02295"
     CDS             complement(24953..>26793)
                     /gene="polA"
                     /locus_tag="P5335_02295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRZ73742.1"
                     /translation="EAKPNSYYATLETTEHFYQSIQGDLPVKLLLQNLMNQTSVCFDT
                     ETTAIDALNAELVGLSFSWEKGKAFYVPFPENKNESQQLIEKFRPFFESETIEKIGQN
                     IKYDLKILANYGISVKGKLFDTMIAHYLINPDMRHNMDILSETYLKYSPKSIETLIGK
                     KGKNQKSMRDVNLEDIKEYAAEDADITFQLKEVFSPILDKAETKKLFDEIEIPLIPVL
                     AAMESEGIRLDVDFLKKMSVEMQKDITDFEQKIYETAGEKFNLASPKQLGDILFDKLK
                     IGGAKQKKTKTGQYATGEEVLSYLVNEHQIVKDILEWRQMVKLQSTYIDALPNQVDPK
                     TQRVHTDYMQTVAATGRLSSNNPNLQNIPIRTERGRLIRKAFIARDENYTLVSADYSQ
                     IELRIIAALSGEENMIKAFQNNEDIHKSTAAKVFNVPLEEVTKEQRSNAKTVNFGIIY
                     GVSAFGLSTQTNLSRKESAELIDAYYATYPKLKAYMANQVDFAREHGYVQTVLGRRRY
                     LKDINSANAMVRSGAERNAVNAPIQGSAADIIKIAMINIHQKLTSEKWQSKMLLQVHD
                     ELVFDVHNSELEKIQPMIKHEMENAFKLTVPLDVELGMGRNWLEAH"
BASE COUNT         8799 a         4240 c         4651 g         9003 t
ORIGIN      
        1 gcattgtttg gaatatagaa ttttttatta aaatcctgaa actctttaag cgttgcagca
       61 tctaaatgat ccatcgaacc aatagttgcc caacgataag gatggtttgt aaacatgttg
      121 cgttttactt ctgcaattaa attcccatat ggttggttgt caacccgaag tcgtttttct
      181 tctttaacaa cttcattttg agtgtctacg ccaatttgat taattaccgg gtgaagtaaa
      241 cgttctgatt ccatccatag tcctaattca agattattag acgggaaaat ttcataataa
      301 taagttctgt catctgaagt attggcgttg tttgttccac catttccggt tacgatttta
      361 aaccattctc cacgaccgat attttgtgtt ccttcaaata aaagatgttc aaaaaagtga
      421 gcaaaacctg ttctttcagg gttttcatct tttgcaccaa catgatacat taccgaagtg
      481 atgacaaccg gtgcggaatt atcttgatgt aaaattacgt gtaacccatt gtctaaagta
      541 tattcttcaa aggtcacttt ttgggcagaa actgttcctc caagcataag cagtgtactt
      601 aaagccatta atgatttttt cataaaaatt ggtgattaat ttttttgcat ttcaaattgg
      661 tatgcaattt cggtattttg ttacagtttt actaaataat tcacaaaata ttttggttta
      721 gataatgcaa ttttagtgta aataatttat ttttaaaatt cagttgtgtg attgagaatt
      781 agttgtatat ttgcaacctt aaaatttaat taaaacacat tgttatgtac gcaatcgtag
      841 agatagcagg gcaacaattt aaagtaagca aagaccaaag agtatttgtt catcgtttag
      901 catctgaaga aggaagcaaa gtttctttcg acaaagtaat gttgttagac gaagatggaa
      961 caattactat aggcgccccc gctatagaag gtgcttcagt agaagccaaa gtgttaaaac
     1021 acttaaaagg agacaaagta atcgttttca aaaagaaaag aagaaaaggt tacaaaaaga
     1081 aaaacggaca cagacagtct tttacacaaa ttgtgattga aggcataaat gcgagtgctc
     1141 ctaaaaagaa agctgctaaa aaagcggaaa ccacagaaga ataattaaca tttaaaactt
     1201 aaacatcatg gctcacaaga aaggtgtcgg tagttccaag aatggtagag aatcagaatc
     1261 gaaacgttta ggcgttaaga tatatggtgg tcaagcagct attgcaggaa atatcattgt
     1321 aagacaaaga ggttctaaac ataatccagg agacaacgtt tacattagta aagatcatac
     1381 tttacatgca agagttgacg gagtggttaa gtttcagaaa aaaggtgaca acaaatcatt
     1441 tgtatctgtt attccttttg aagcataatt agaatctact tcaaaatata aaaacccatt
     1501 tcgagagaga tgggtttttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 taattagaat ctacttcaaa atataaaaac ccatttcgag agagatgggt tttttatttg
     1681 tgataggaaa atatttaaaa tttggttatt caaagtgaac tgtcttatta attatactat
     1741 tttcacttag gatggattaa ataaaaatcc aatactaaaa taaagattag tttcatctat
     1801 atcattaacc catgaggcat caaaagtaat tgggcccaaa agagaaaggt aggaaatgtt
     1861 agccccaccg gaaaacaaga aactagtttc agttcgttct tgccatttgc ctttcggaga
     1921 aaaagcatca tccatataat cattaaaagt cttgaatcct actgaggcta tatcaatgtg
     1981 cggagtcagg taaatttttc cggtcaagtt tgtttgcaaa ccaacattca ttttcatcag
     2041 ttgtgttact gtcaattcat cttcatttaa tcctggaaaa acagttctgt tacttgttgg
     2101 acttatagat gttcctccta aaaaatattt tgctccataa ccgtgtctgg aaaaggatac
     2161 ttcatctgat tttaggctat cttcaaaaat aaaattagta gtagcaccaa taattcccgt
     2221 taattttctt tgtaaaggaa ttcttttttc aaaagtcaag cctaattttg taaacccatt
     2281 tgtagctccg gagatttttg gtattgttac atcagtgaaa tttaattcaa catcatgcaa
     2341 taatgatctg cttatttttg cttgaaaaaa ggtcccttta ttggcaaaaa aaactttatc
     2401 caaatcatta taagaaaaat gaacacctag ttcaatattg ttgaagtaat aacttttaaa
     2461 tggatattcg gtcaaatcag catctgcttt aggttttaaa taagtatatt gataatttaa
     2521 agacatcccc acgtaggttt taaatgaatt taagtttctg tttacttgat tattgaattg
     2581 ataagagcga tattttaaat ccgtcacaag tttattgtct acaaataatt cctgaattaa
     2641 attttcacca taaagttctg tattccacca ccattctttt tttgaaccaa aaatatcttg
     2701 atattctatt ctcgctcgtg gttgttccgc aatgtcggcg gtaatcaaaa aacgagatga
     2761 ctcaccaaga atatttctac cggtataatt aagaataact ccaacacctc tgtaagtatc
     2821 ataatgaaga gaagcattta cttgattttt agaattttca aaaaaggtta tttcaaggcc
     2881 tattttacca tcatttacaa aagaactagt tgtaatttta ctgaataaat ttgttcccat
     2941 tgctctattt attccttcaa ctaaatcatg ggatacatat tttgtgtgag gttttatatt
     3001 cattctttct ttaacaaaag caaggttttc tttactgatg tttgtgaaaa ctatcgtgtc
     3061 taaggtaaat tcttttggga cgttaggaag ttcatgggtt ctttgtttaa atttatttaa
     3121 tttttctgcc aaagcgacta gagcatccat gttttgattc gttgcaattt taccttcttc
     3181 atagatttca ttgctttttg caaaatctcc tgtagaatat ttcaaatttg gaagatggtc
     3241 aaccaaaata gtacagagtt ttcttttttc tgggtctttc acgttgctgg tcaacatact
     3301 tgtttgaacc aaaacagaaa caaaattatt caatttttct tttggttcca tgccaccgcc
     3361 cacatcacta ccgattataa tatcagcacc taattgttga gcaatatctg ttgggaaatt
     3421 attcacaaca cctccatcta ccaagagtgt ttctttataa ggaacaggat taaatactgc
     3481 gggcaaagac atgcttgccc gaatagctgt tgcaagattc ccactgtcta ccactacttc
     3541 ttttccggta actaaatccg ttgctaaagc tctgaatgga atagaaagtt tgtcaaaatc
     3601 agttatattg taaacaggat aagttaattc tgaaagatac tctcttagat tttgatcact
     3661 caaaatagag ctaacacttg tgggttttcc ttcttttata tcaagaccta tcaaatatct
     3721 ttcaaattca cttttttctt ctacgcttac tttgtcaaat gaaactcctc caccaagaag
     3781 tttgtcccaa tccatgtttt ttgtcatttt agcaatgctg tctccggaat accccatggc
     3841 gtaaagcccg cccatcacgc ttcccatact atttccaaca ataaggtcag gaactatgtg
     3901 taacgaatct aaagcctgta gaagagggat atgtgcaaca ccttttgctc caccaccact
     3961 taataccaaa actactttag gttttttatc ttgagcgaaa tttagttgtg ggaaaataag
     4021 aaaaaaggaa agagtgagta agaataaata atttttcatg gttcattatt taatagtaaa
     4081 acaatacaat caatttatga agattacttt catcaaactt caacaaatct ttagtaaagt
     4141 tagctatttt taagaaaatt atttatcaga agttactatt aattcaataa aataattaat
     4201 tgctacaaaa aatgattata tctcatggaa aatctttaga gattttttcc agttaatctt
     4261 tattaaaaaa aaggaaactc aactttctca aattatatca caagtttaaa caaaaaaccc
     4321 tcacttttca gcaagggtca attttatttt tagtcttaat aaaacaaaag acttaaaact
     4381 aatatctgta atattcaggc ttgtatggtc cctgaacatc cactccaata taagcagctt
     4441 gttcttcatt caatgtttcc agttcaatgc ctaatttagc caagtgtaaa gccgctactt
     4501 tttcatctaa gtgtttaggt aacatataca cttcgttttt gtaattagct gaattattcc
     4561 acaactcaat ttgagctagg gtttggtttg taaatgaatt actcatcaca aaacttgggt
     4621 gacctgtcgc acaacctaag tttactaaac gaccttcagc caaaatgatg atatcttttc
     4681 cgttgatggt atatttgtct acttgtggtt tgatttcaat ttttgaagtt ccgtgattac
     4741 tgtttaacca agccatatca atttcgttat caaagtgtcc gatgttacaa acaattgttt
     4801 tgtctttcat ttgttcaaag tgagaaccta caacgatatc tttatttccg gtagttgtga
     4861 tgatgatatc agcatttcca actacagtat ttaatttttt tacttcaaaa ccatccatgg
     4921 cagcttgtaa tgcacaaatt gggtcaattt cggtaaccgt aacaattgaa ccggcacctc
     4981 tgaaagaagc agctgttcct ttaccaacat caccatatcc gcaaacaatt actcttttac
     5041 ctgccaacat aatatctgtt gcacgacgaa cagcatcaac agccgattct ttacaaccgt
     5101 atttgttgtc aaatttagat ttagtaacag agtcatttac attgatggct ggcatatata
     5161 aagtaccgtt tttcattctt tcatacaaac gatgaacgcc tgtggttgtt tcctctgaaa
     5221 gacctttaat tcctggaatt aattctgggt aacgatcaaa aaccatattg gttaaatcac
     5281 caccatcatc taaaatcata ttcaatggtt ttctgtcttc accaaagaat aaagtttgtt
     5341 caatacacca gtcaaaagac gcttcatcaa gacctttcca agcatatact tgaattccgg
     5401 cagcagcgat tgcagccgct gcttggtctt gtgtagagaa aatgttacaa gaactccaag
     5461 tcacttctgc tcccaaagca attaatgttt caatcaaaac tgccgtttga atggtcatgt
     5521 gtaaacaacc ggcaatacgg gcacctttta aaggttggct attgccatat tcttcacgaa
     5581 gagccattaa acctggcatt tctgcttcgg ccaattcaat ttcttttctt ccccaagcgg
     5641 ccaaagaaat atctttcact ttgaacgcaa cataaggtaa tgtttgtgta ctcatttata
     5701 aaatagttat attagctaaa aatttttgca aacttacgca tttagataag attttaaaaa
     5761 cctttttagg ttttttgtga tttgtttaat acgctaaagt tttgaaatac aaattgatat
     5821 ctttttaaaa accatgcctt ttcataaaaa aatacacgtt gattcaaaaa cagttgtttt
     5881 agtttggaaa atttcagaat cgtataacga tctattccgt tctgtttcac tgaaagacaa
     5941 ttcgttagct cgcatggaag gaatgaaatc agaaagccat caaaagggtt ttttggcggt
     6001 tcgaatgtta ttgcaacagg caggttatac cgattttgat ttgtattatg atgaatttgg
     6061 gaaaccgcat ttaaaagatg ggaaacatat ttcaatctct cattcatttg atttttccgt
     6121 tattgtttta tcagatagaa atattggaat cgatttagaa ttgcgtcgtg atttagtcaa
     6181 aaaaattgcc cgaaaatttg cagccgagaa atttgtatac gaatcagagc ataattccga
     6241 tgattttatc cgtaaattaa ccgttatttg gggagtgaaa gaagccgttt ttaaagtaag
     6301 aaatgaaata ggaattagtt ttaaagatca tatttttgtt tgcccttttg aaatggatga
     6361 taaacaaacg acaagtttat tagattttaa tgatactaaa caagaatttg aagttttctt
     6421 tacagaaatt gaatcttata ctttagtttg gctttgggaa actgaaaaat gaaaaaaata
     6481 taccaagaaa tagtacaagc taaaattgac aatcgaaagc ttttagccat tttactcgat
     6541 ccggataaag tagattttga tggtttattg ttgttgacag ataagattaa aatatctctg
     6601 gcaactcata tttttgttgg aggcagtatt gtagaaacca ataaaattga cttattaatt
     6661 caatttttaa aagataattt aacgattcct attttgcttt ttcccgggaa tccatctcag
     6721 atttcaactt atgcggatgg aatattgttt ttaagtttac tttcaggaag aaatcctgtt
     6781 tttttaattg atcatcatgt aaaagcagtt ccaaaattaa aaaacacatc attagaaatt
     6841 atacccacgg cttatcttct cattgaaagt ggtagtcaaa cagcggttga acgggtaagt
     6901 caaacggaac cgttagacag aaataatagt aattatgttt gtcaaacggc tatggcagga
     6961 cagtttttag gcaataaatt aatttattta gaagccggaa gcggagctaa attagccgtt
     7021 ccattagaaa tgattcatga agtttcaaaa tgcatttcga ttccattgat agtaggagga
     7081 ggtattcgaa cgcttaaagg aattaaacaa gcctacgatg ccggagcaga tttggttgtg
     7141 ataggtactg cttttgaaaa tgatgtaact ttctttgaaa atgattgatt ttcttttttc
     7201 tcaatataaa acatactcaa gttttcatat ttatttggaa attgtcgcgg tactttttgg
     7261 tttagttagt gtttggtatg ctaaaaaaga taacatttta gtattcccaa ccggaattat
     7321 tagcacgaca atctttatat atttgctctg gaaatggtcg ttattaggag atatgtcaat
     7381 aaatggttat tataccatca tgagtattta cggttggtat cattggactc gaaaaaagaa
     7441 taatcaaacg gtgtttccaa ttgccaaaat ttctgaaaat gaaaagaaat gggcaattat
     7501 tatttttata gtttcatcga ttggggtaat tttaatctat cagttttttg ataaatttac
     7561 acaatggaca gcttacgttg atactttaac aacaggactt ttttttgttg gcatgtggct
     7621 catggctaaa cgaaaaattg agcattggat tttttggata attggtgata tcatttcaat
     7681 tcctttgtat ttttgtaaag gttatacatt tacgagttta caatatttaa ttttcacaat
     7741 cattgccttt tatggctata tcgaatggaa gaaaatctta aacagcaaca cacaagcata
     7801 attaaaattg ctatttacgg accagaaagt acgggaaaaa caaccttagc aaaacaactt
     7861 gctgagcatt ttaataccgt atggataccg gaatttgcaa gagattatct tcaggataaa
     7921 tggaataaaa cgaaacagat ttgtcaacaa gaggatttgt tacccattgc tattggtcaa
     7981 acgaaacttg aaaatgaagg attacaaaag gcaaatcaag tattgttttc ggacacaaac
     8041 ttgatggtga ctaaagtttt ttcagatatc tattacggaa atacaaatcc gatattacaa
     8101 aaggcggcta aaaagcataa atatgatttg tttttactga cggatattga tgttccatgg
     8161 gaaaaagacg atttgcgtga cagaccggat aatagagaag atacatttaa actatttgaa
     8221 gatgctttaa ttcaatacaa taaaccgttt attaaaattt ctggatctaa agaaaagaga
     8281 tttcaaaaag caacgttatt aatcaatgat tttttagaag caaaagggag agggtttaat
     8341 tcttttgatt ttatacaatt ctatcagcga aatgtagaat tagataaagt aaaaaatcaa
     8401 atccattttt taaccgaagg aatacctaga attgatttag accgaatagc caaaatagat
     8461 gatggaattg aacagctaag caaagaagag gcaatttatt atgctaactt ttttgataac
     8521 aaaaaaaatc agttaaaact caaaaagttt gttcctgctt ccggtgcagc cagtagaatg
     8581 tttaaatttt tgaatgattt tttacatgat tttaaattag gagaggaatc aatcaatgct
     8641 tatatcaata gaaaaaaggc aaaaaattta tcgctatttt taattgggat agaaaaatta
     8701 ccgttttatg aaaaggtttt atcacgagta aaaattattt ttccggaata tgaaagttgg
     8761 ggatatgaca gtaaaatgtt tgccttcatt aaaattatgt tggctaaaaa cgaatttgat
     8821 tttgctaata aacctaaagg tgtgttgcct tttcacaaat ataaatcaca ttgtgcaact
     8881 cctattgaag agcacttgaa cgaagcagtt tattatgctg cttccaatca aaaagcacat
     8941 ttacatttta ccgtttcaga agagcatcaa gaggattttg aaaaaattgt tgaatcagta
     9001 aaacccaaaa ttgagaacca atttcaaaca gaaataaacg taacctattc ctaccaaaat
     9061 aaagctaccg atagtatagc attagatatg gagaacaatt tatttcgtga tgatcatgac
     9121 ttgttagttt tcagaccggg tggacatggt gctttaatag aaaatttaaa tcgtttagat
     9181 gcagatataa tctttattaa aaatattgat aatgttatcc aaaaccacat tgaagaaatt
     9241 gcgttataca aaaaagcttt agcggggatt ttattagata aacagcaaca aatcttcctg
     9301 tatctttctc aattagatac agataaagtg gataaagttt ttttagatga tgttgttact
     9361 tttattcaaa caaaacttca ttttgaagta gtagaagatt tttcaaaata tactaaagaa
     9421 agtaaaattg actatttgaa aaattattta aatcgcccga tacgagtatg tggaatggta
     9481 aaaaatgaag gagaacccgg aggcggacct ttttgggtac gcgataaaaa aggccttgtc
     9541 tttttacaaa tagttgaaac aactcaaatt gatgagaccg atgcaacgca agttaaaatt
     9601 ttagaaagtt caacccattt taatccggtt gatttagtat gcggcattcg aaactacaag
     9661 ggtgtcaact ttgatttgaa cgaatttgtt gaccataata gcggatttgt tgttcataaa
     9721 aacaagggtg gaaaagagtt aaaagcttac gaacttcccg gactttggaa tggtgcaatg
     9781 gcaaaatggc tgaccgtttt tgttgaggtt ccgttaatta cttttaatcc ggtgaaaact
     9841 gtgaatgatt tacttaaaac agctcatcag ccacagtaat ggatagtgat aaaattatag
     9901 cggaactaaa ttttaaagcc gttagaagta gtggggcagg tggtcaaaat gtgaataaag
     9961 tttcttctaa agttgttttg tcttttgatt tgtttaactc acaatgttta tctgatgatg
    10021 aaaaaacagt acttttcgag aaattaaaaa caaaattaac tgtagaagga atattgatat
    10081 taaactgtga tgaaaatcgt agccaactca aaaacaaaga aattgttaca aaacgttttt
    10141 tgaaactgat tcaaaatgcg ttgataattc caaaaaaaag aaagctcaca aaaattccac
    10201 gttcagttat agaaaaaagg attaaggcaa agcggaacac ctcggaatta aaacaaaatc
    10261 gcaagagacc tgaagcttag aatacagttt ttgaaagcga atataatatt aacttttgtt
    10321 gaagaataat ctttagaaaa ttataactat ttttgctttg tctcaaaggg gtgctctaaa
    10381 aaacgagctg agatcatacc caacgaacct agaacaggta atgctgttta gggaaaagac
    10441 aaaaaaaaca aaagtgcacg tttgtttgac tgtcaagagt atcaatttta atttttaacc
    10501 aagaataatt acccctttta ttcgtaaaca ttttacgaat gaaaacttta ttcaacaaat
    10561 tttgccttaa aggcactaag tcgtcaggta ggtgttgttt tcttagctta cttctatttt
    10621 ctttttccgg attttcacaa gaaacggtat atgatacaac taaaacaaaa gatatttcac
    10681 ttgatgaagt tttagtatca gccattcggg tgacagcaca atctccggta acattttcta
    10741 atcttcgcaa agaagagttc caatccagaa atttgggaca ggatattcca ctcatgatga
    10801 attatctacc ttcggttgtt acaactaccg atgccggtgc cggtgttggt tataccggaa
    10861 ttcgggttag gggttctgat gcgacacggg tgaatgtaac aattaatgga attccgtaca
    10921 atgattccga aagtcaggga actttttggg taaacatgcc cgattttgcg agttcaaccg
    10981 aaagtttaca actacaacga ggggttggaa cttccacaaa cggagccgga gcttttggag
    11041 caagtctgaa tgtgttaact gataattatt cgaaagaatc caaaggtgaa atttcaaatt
    11101 cctttggtag ttttaacact cgaaaacaca ccgtgaagtt cagtaccggt ttgatgaatg
    11161 agcagtttga aattgcggga cgcttatctg caatcaattc cgatgggtat atcgacagag
    11221 cttcatctga tttgaaatcg tattttttac aaggaaccta tgtgggcaat tcgactttaa
    11281 tcaaaggttt ggtgtttgga ggcattcaaa aaacctatca agcttggtac ggtttggaag
    11341 atcccgaaaa acttgaaaat gatcgaacgt ttaacgttgc cggaatgtat tatgacgaaa
    11401 atgggaaaat gaaattttat gacaacgaga ccgataatta ccaacaagat cattaccaat
    11461 tgcattggaa cgaacgctgg agcgaacatt ggagcagtaa ctttgctttg cattatacca
    11521 aaggaaaagg ttattttgaa caatacaaag aaaatgaaga tttttcagat tatggtttag
    11581 aacctattgt tttgggtgga gaaacgattg atatgacgga tttaattcgc agacgttggt
    11641 tggataatga tttttacgga actacttttt ctgtaaatta taacaaagat aaagtagatg
    11701 taattattgg tggtggagca aataaatatg aaggagctca ttttggcgaa attatttggg
    11761 ctcaatatgc ttcgcaaagc gaaattagag atcgctatta caatgatttt tcttcgaaaa
    11821 cagatgtgaa tgtttttgca aaagtcaact atcaactcac aacaaaatgg cgattgtttg
    11881 gtgatatgca ataccgaaac gtaggctatc aagccaatgg agcagaaaca ggcttagtaa
    11941 acgataagtt taattttttt aatccgaaag caggtgtgac ttttgtattg aatgaaaaac
    12001 aaaactttta tttttcatat tctaaagcca atcgcgaacc caatcgaaac gattatgaaa
    12061 acggaaatcc aaaaccagag aaactggatg attatgaatt aggttggcgt ttctccaccg
    12121 aaaaagcaaa attgaatgtc aacggttatt tcatgaaata caaagaccaa ttagttttaa
    12181 ccggcgaatt aaacgatgtt ggtgctcctg ttcgagccaa cagtggcgat agttaccgac
    12241 ttggattgga agttgatgca acttttaaac ttaccaataa gtggtttttg caaccaaatg
    12301 taacgctgag tcaaaataaa aatcaagatt ttgtatttca acgggacgga gtttttaaag
    12361 atttaggaac cacaaatatt gctttttcac ccgatttaat tgcggcaaac agattggtgt
    12421 ttatgcctat tcaaaatttc caagtgtcac ttttgtcaaa atttgtgggc gaacaatata
    12481 tggggaatat cgatgcagaa agttcaaaat tagacagtta ttttgtgaac gatttgaatg
    12541 tttcgtatga aattgtaact aaaaaagtat tcaaatccat aagaattgat gtgttgataa
    12601 ataacatctt caatttggag tatgaaagta atggctattt ctacacgtat gacgatgatt
    12661 tttccgatcc gggaactgtc acaactattg aaggtgccgg tttttacccg caagcaggga
    12721 taaacttatt agcaggatta acactcttat tttaaacaca gcataaaaaa tcctgactgt
    12781 tcggtcagga ttttaattta attataaaca cgtatgtttg gtaaatttac ctccactcga
    12841 tatcttttca tggggtattg caaactgcta agccctgtaa ataagctata ttgttcatca
    12901 tcacaaggac atatggcgtt aattccactt attaacattg tagaacaacc tgaaataggt
    12961 tgattagggc atgctgcatc ataggctaaa taaccgctgc cactattaaa aacaattact
    13021 cctcttgccc cagcggtgcc ttgtggaata tagacggcat tactagggaa ttgtaaatta
    13081 ctataggaag gtaaattcac attgatatca actgtaaagg tataatttgg aatataagga
    13141 ttattgtcat ttccacctcc actgtcgcaa ccgaataaga ataaagtact gaataaaact
    13201 aaaatgattt ttttcatttt tttgattaaa atagagtgaa caaatttaaa ttaattacct
    13261 ttgattaata ctaataagca ataatttttc tgagaaacaa aaaaatatcc tatctttgtg
    13321 ataagaaatc ctctcaaagg ggattttttc tattttatat aaacgagaaa taaaagattt
    13381 ttatataatt aagttcactt ttttgtgact gattaacaaa caaaatacag cattaaaaaa
    13441 cacatgaagt tatgagtaca gtatcgtatt ataccgctga gggtttaaaa aaattaaaag
    13501 aagaattaga aacgttaaaa catattgaac gaccaaaagc gtctcaagcc atcgcagaag
    13561 caagagataa aggagattta tctgaaaatg cagaatatga tgcagctaaa gaagcacaag
    13621 gtcttttaga attgcgtatt tcaaaaatgg aagaggttta ttctaacgca agattaattg
    13681 acgaatcaca attagatatg tctaaagcat tggttttatc aaatgtaaaa ataaaaaacc
    13741 aagccaatgg tatggaaatg aaatatacct tggttgcaga aagtgaagcc gatttaaaat
    13801 caggtaaaat ttctgtaact tctccaatag gaaaaggctt actcggaaaa tcggttggtg
    13861 acgtggcaga aatcactgtt ccaaatggtg tattgaaatt tgaaatatta gatatttcca
    13921 gagattaatt tgtgaaatgg caagtatatt tacaaaaata atcaatggtg aaattccttg
    13981 ttataaaata gcagaggatg ataattttat tgcattttta gatgtaaatc caaatgcaaa
    14041 agggcacaca ctgtgtgttc caaaacagga aattaacaag ctttttgaaa tggaggaaga
    14101 tcattattta aatttgatga aattttccag aacaatagcc gtggctttag aaaaagcaat
    14161 ttcttgtaaa cgaattggaa tgagtgtaat cggtttagaa gtgcctcatg ttcatgtaca
    14221 tttaattcca ctcaatgaaa tggatgaagt tcgttttcaa aaaaaggttt ctttcacaaa
    14281 agaagaattt gaagaaatag ctaaaaatat taaagctaat ttataattta aatcccgtga
    14341 aatcgggatt ttttcatttt atgaagttgt ctttttaaaa cttaacatca ttgtagtgcc
    14401 tttatcaatt tctgaatggt gcactttgat ttttcctttg tggtattctt ccacaatgcg
    14461 tttggttaag gaaagtccca atccccaacc tctttttttg gtagtaaatc cgggatcaaa
    14521 taccttttta aattgatttt taggaatgcc ttttccgcta tcagaaacat aaatccgtac
    14581 ttctttttct tcttcaataa tatttaagtc caattttccg cgacctttca tcgcatcaat
    14641 ggcattttta actaaatttt caatagtcca actatgtaaa gcaggattga tagatacaaa
    14701 tattggatgt ggaggagcaa gaaaagtaaa ttctacttgt tttgaaaacc gagatttaag
    14761 gtaatcatag gattgttctg tttcagctat aatgtctaat ttttccaaaa cgggttcaga
    14821 gcccacttta gaaaatcggt cagtaatcgt ttgtaatcgt gtgatgtctt tttgtatttc
    14881 caatagggta gattcagcaa cattttcagc tttcaaaatt tccaaccaac caataagtga
    14941 tgataaagga gtgccaattt gatgagctgt ttctttagcc atcccggccc aaagtttatt
    15001 ttgtgtagca attttagaag tttgataaaa attgaaaacc aataatccaa aaagaaaaat
    15061 aatcaacaat aaagcaagtg gataatattt caatttattc aataatgagg aatcaccata
    15121 atataaatag cgaaattttc cctcgacata ttccatcaca atcggatcat tttccttttt
    15181 taatttttct aataagctcc tgagttttgc agaatctttc tcgtcaatat cttcaatgtt
    15241 tgttttgttg acaatgctgt cgttttcatc ggttacaatg attggaatag ttgtcgtatt
    15301 gctaataatt tttagaggaa gactaatatc tgcatcaaca tctgaactga aaacattagt
    15361 ttgtgcttct gcccaaagtt ccattttcaa acgttcatct tttttaaaac gttgaaaaaa
    15421 agtataagta ttccaaagta ttagcgaaac tatcacaaac gacgtgagta ttaaaatcca
    15481 ccgggtgaca tttcgacttt cggaaaactg cataaaaaag tatttaggat aaaagtagta
    15541 aaatcttatt ggaattaaaa aaatcgaacg gcaaatactt ttcaatttta tacatttgta
    15601 gaaacacaaa tataacatgg ttagtatttc tcctaaagat atttctccgc taaaattaca
    15661 aggctattta cagagtgctg taggtccaag accaattgca tttgccagca cgcttgacaa
    15721 aaatggcaat cctaatttat ctccatttag tttttttaat gtgtttagtt cgaatccacc
    15781 ggttttgatt ttttctccgg ctcgaagagt tcgtgacaac acgataaaac ataccttaat
    15841 caattgtgaa gcaacccgag aagttgtgat taatgtggta aattatgaaa ttgtacaaca
    15901 aatgtcactt tcgagtacgg aatatgccga tggagtaaat gagtttttga aatccggttt
    15961 aactatgctg ccttctgagg ttgtaaagcc ttttcgagta gcagaaagtc cggttcaatt
    16021 tgaatgcaag gtgaatgaaa taatcagttt agggactgaa ggtggggccg gaaatttaat
    16081 catctgtgaa gtagtaaaat tgcatattga cgaatcgatt ttggacgaaa acggaaacat
    16141 agaccaacac aaaattgatt tagtatcaag gatgggtgcc aattggtatt cgcgttccaa
    16201 tgaaggtctt tttgaaattc caaaaccatt ggcaacgtta gggattgggg tagatcaaat
    16261 tccggatttt gtcaaagaaa gtcctgtttt taacgggaat gatttgggaa tgttagggaa
    16321 tgtagaaagt ttgccaacca acgaagaaat taatatattt gtacgagata atattcaagt
    16381 aaaaggcgtt ttgagttctg ctgatgaaat gaaagtgcat gaaaaagcaa aagaattcct
    16441 caataaacaa gatgctgttt cagcttggaa ggtcttatta gccaaacgag tttaatataa
    16501 gccaatttaa taatttttaa aatcaagagt atggaagtta caggaaaaat cagagtagtg
    16561 aatccggaac aacaagtgag tgctacattc agaaaaagag aattagttgt ttctacagaa
    16621 gagcaatatc cacaacacat cagtattaat tttgtacaag ataaatgcga tttgctgaat
    16681 agttataaag tgggtgaagc tgtaaaagtt tcaatcaatt taagaggtag agaatgggtt
    16741 aacccacaag gtgaaaccaa atatttcaac gatattcaag gatggagaat tgaacgtttg
    16801 caagcagaag ctcctgctgc aggaatgcct ccgatgccgg cggcagaagc atttgaaccg
    16861 gcaactaact ttaatgaaga agagcatgat gatttgcctt tctagtgaga gaatagaaga
    16921 aagaaataga aaatcattct aaatggctaa cttggttatg cgtttagaat gattttcggt
    16981 tttagcaata tatctctttt gtttatctat cttcaataac atgtattttt tatctactga
    17041 attatatttt ccgccagtaa cagaagcttc ctcggaagga attctcgcag tgggaggcga
    17101 tttgagcata gaaaggttgc tgttggctta tcatagcgga atatttcctt ggtttgataa
    17161 cgatgaaccg attttatggt ggagtccgtc tgagcgaatg gtagtgaatc ctcaagatta
    17221 taaaatagcc aaaagtttgc gtaatatttt aaatcgaaat atttttaaag taacatttaa
    17281 tcaaaatttt acggaagtga ttaaaaaatg ccaaaaaata aaaagaaaag gtcaacgcgg
    17341 aacttggatt accgattcaa tgttggaagc gtatataaaa ttacatgaaa caggatttgc
    17401 aaaatcggta gaagtttggc aagataacga attggttggt ggtttatatg gagttgattt
    17461 gggcaaagta ttttgtggtg aaagcatgtt ttcaactgtt tctaacgcga gtaaagtggc
    17521 ctttgtttca ttaatacaaa aacttaaaga ggaaaattac catttgttgg attgccaagt
    17581 gcaaaatgac catctagaaa aattaggggc ttttgaaata tcgagagaag tttatatgaa
    17641 ggttttaaaa gggtaaaatt atttattatt tcccaattcg ccaactatat tctttaactg
    17701 atggaatata agcacggtca cctttaatga ttaaatctgt gtagatttta ccatcatatt
    17761 ttatgccaat ggcagggcct gatgtgtaga caatattatc cttaaattca acttttattc
    17821 tgtcatcatt gagttttacg tcagatactt tttcaactaa ccaggttgat ttccattcga
    17881 tatataattc gttttcggct gtttttttta cacttttaac tatggaagag gcatcttttg
    17941 gtagcttatt ctgtttgatt aattcttcct ttgttaccgt ttgtccaact tgaatagtat
    18001 ttaatatagc tgacaaattg gtgtaaattt tatggttttt cggaatggca actgattttg
    18061 attctgtttt tcttaaagtg ggaattggct cagtagccgc tatgacaggc tctttttttg
    18121 tgattttcga atcaattttt tgagttgttg agttttcaac tttttcttca ataattaaag
    18181 atgaattgtt atctttttca acagtttgaa ttttttcgta cgaagaatct gttttctttt
    18241 catttttgcc acaatttaaa aagctaatca gtagaaaagt aaataaagtc aatgaagcaa
    18301 atttcttaaa catgtctgaa tgttttttat ggagttttta tataatgagt gtaaaattac
    18361 tatttaaatt acttgtgggt taaacaaaat caaaaaactt gagagctaaa atgataggct
    18421 aaaaaaatgt tttttatttt cttttccagc aactcaacaa atgatcattt accattccgg
    18481 tggcttgcat gtgtgcataa actacttttg aacctacaaa tttaaaacct ctttttttca
    18541 aatctttgct aattgcatcg gatagcggag tcgatgccgg aatatctttt aattctttaa
    18601 attgattcac taaaggtttt ccgtttgtga aattccaaat atatgtagcg aaagtgccaa
    18661 attcttctat cacttttata taagcattag cattacttac ggcagctctt atttttagtt
    18721 ggtttcttat gatgccggta ttctgcatta attcctgaat atgattttca gtaaaaagag
    18781 ctactttttc atattcaaaa ttttcaaaag ctattctgaa attttcacgt ttagccaaga
    18841 cggtgtacca acttaatcct gcttgaaaag tttctaaaaa tagaaattca aataattttt
    18901 ggtcatcata aactggattt ccccattctt catcgtggta agtgcggtat aaatcatctt
    18961 tttcgcacca agcacatctg tttttaattt ccatatcagc ttcattttta ccaaatataa
    19021 aaaaagaaac ccataatttg tgtttttctt tttatttagg ggttgttctt taaaaaatca
    19081 ataaaaatta aaatatagta ttaaaaatag gggttagttg tgtaaatttg ctaaataata
    19141 tacatttaat agtatgaaaa taggggttcc taaagaaata aaaaataacg agagtcgtgt
    19201 tgggatgacc cccgcaggtg ttttcgaatt gataaaaaac aatcatacag tttacgttca
    19261 acaagatgct ggttttggaa gcggattttt tgatacggat tatgctgttg taggagcaat
    19321 tttagtgcct acagctgctg aagtttatgc acagtccgat atgattgtga aagtgaaaga
    19381 accaattccg gaggaatatc cattgataaa agaaaatcat gtagttttta cctattttca
    19441 ttttgcttca agtgaagaat tgacaatcgc gatgattaat tccaaagcgg tttgtatagc
    19501 ttatgaaaca gtggaagaaa cagacggttc gttgccatta ttaacgccaa tgtcggaagt
    19561 tgccggcaga atggccattc aacaaggggc aaaatattta gaaaaaccga ttaaaggacg
    19621 aggcgtattg ttagggggtg ttcctggagt tcctcccggg aaagttttgg tgcttggagc
    19681 tggaattgtt ggaattcaag cagcaaaaat ggctgccggt ttaggagcac atgtgactat
    19741 tttggacatt aatatgaaac gtcttcggta tgtaaatgat gtgatgccta gtcatgttgt
    19801 gaccgaattt tcaagtgagt ataatattcg ccgacatatc aaaaaccacg atttaataat
    19861 aggggctgtt ttaattccgg gggcgaaagc tcctaaattg attacccgtg atatgttgaa
    19921 agagatgcac ccggggacag ttatggttga tgtagcagtt gatcaaggag ggtgttttga
    19981 aacaacagta gcaacaacac atgaaaaccc aacctatatc attgatgatg tagtgcatta
    20041 ttgtgtggca aatatgcctg gagcagtgcc ttatacttct actttagcat taacgaatgt
    20101 cacgttgccg tatgtgttga aattggccaa tttaggatgg gaaatggcca ctgaacgata
    20161 tcctgactta agaaaaggac ttaatattat tcatggcgaa ataatatata aagaattagc
    20221 tgatacgttt aattttagtt aataggtttt gtttgtttgt tttaaaaatc ccatcaactt
    20281 tcaggcgatg ggattttttg ttttatcatt gaaggagact attttttttt tgtaatagta
    20341 tcaatataca gttcttcaac tttttttcta gcccaatctg tttttcttaa aaatgttaaa
    20401 ctcgatttaa tagaaggatt ttcggtaaaa catttaattt ttatcagggt tcctaacatt
    20461 tcaaaaccat aatagtcaac taaatattca acaatagctt gtagtgtttt accatgaaga
    20521 ggatcggtgt tttgcatgaa gtaaaggtaa ttaaaatgca taaaaaacct caatctttac
    20581 aattgaggtt tttaaaatat ttagattctg aaacaaattc agaataagat taccctaaag
    20641 caactctttt gaatcctgta atttcaacgt tgaatccttt aacgtaatcg ctaacttttt
    20701 tgctatcgtc tttgataaag ttttgatcta ataaggcttt ctcttggtct aaagtagtat
    20761 tatcagagat gaaacgttga atttttcctg gaataatttt atcccaaatt tgttctggtt
    20821 tgccttcagc ttttaattca gctttagcat cttcttcagc ttgtttgata acttcttcag
    20881 ttaattgaga gaaagaaata tatttaggaa cattttttaa agtctttcct aaacgttttg
    20941 cttcttcatt ttctttttcg attatagcaa tacgagcagc aagttcagaa gcaacgaaag
    21001 caggatcaaa atctttgtaa gataatgtat cagctcccat tgaagcaact tgcatagaga
    21061 tgtctttagt taaaacgtca ccattagaaa ttggttcaga aattgcagtt aatgcagcaa
    21121 ttttgttaac gtgaacataa gatccaacga aagcaccttc taaaatttca aaaccaccga
    21181 tttcgatttt ttcaccgata acaccagttt gctcgattaa tttttcagca atagtaattc
    21241 cgttgaaatc tgaagctaaa aattcttctt tagaagagaa gttgatagct ctttcaacta
    21301 attgtttagc taaagttaca aaactttcat ttttacctac gaaatctgtt tcacagttta
    21361 aagtaatgat agctccttta gttttgtcag catttacaaa agaaacagca gctccttcac
    21421 tagactcacg gtcagaacgg ttagcagcaa ctttttgccc tttttctcta aggttttgta
    21481 tagctttatc gaaatctcct tcagcttcaa ccaaagcttt tttacagtcc atcattccgg
    21541 cacctgtagt ttgtcttaat ttatttacgt ctgcagcagt aattgttgcc attttgtatc
    21601 tatttttaag tttttaattt ttttgtaaaa aaacaaattc caataatcaa attccaaatt
    21661 ccaattttat taaatcatca atttttagag agacaatttt ttggaacttg gaatttttcc
    21721 tatcggaatt tatagtattt attcttctga agttgtttta gcttcttctg cttccactgc
    21781 tggagcagct tctactgcta ctacttcttc tactgtatct tcaacaacag catcgccatc
    21841 tttttcagat cctctttcag aaagaccctc gatgatagca ccggttacta aagataaaat
    21901 tttatcaatt gatttagaag catcatcgtt agctggaatt acatattcaa cctgacgtgg
    21961 gtcagagttt gtatccacca ttgcgaaaac tggaatgttt aatttttgag cttcttttac
    22021 tgcgatatgt tcagctttga tatccactac aaataatgca gcaggtaatc tagacatgtc
    22081 agcgatagaa cctaagttct tctctagttt tgcacgaaga cgatctactt gtaaacgttc
    22141 tttctttgaa agtgtattaa aagtgccatc tttcttcatt ctatcaatag aagccatttt
    22201 tttaacggct ttacggatag taacgaagtt tgttaacata ccaccaggcc atctttccgt
    22261 gatatacggc atgttcgcgg cagcagcttt ttcggctacg atatcttttg cttgtttttt
    22321 ggtagctacg aaaagtactt ttctaccaga tgctgcaatt tttttcaagg cttcgttagc
    22381 ctcttcaatt ttagcagcag ttttatatag gttaatgata tgaataccat tacgttccat
    22441 ataaatgtaa ggagccatgt taggatccca tttacgagtc atgtgtccaa aatgaacacc
    22501 agcctctagt aagtctttaa cttcaacttt gtttgccatt tttgtactag tttacgttct
    22561 gttgattagc aataatgtag tagcgattac tctggtcttc attatttaga tgctaaacta
    22621 attttccgat cgcatcggaa taataacaac aataactttt ttaataataa ttttgtaaat
    22681 cgattaacgt ttagagaatt ggaatctctt acgagctttt ttctgtccaa atttcttacg
    22741 ttctaccatt cttggatctc ttgttagcaa tccttctggt ttcaagatag ctctgttttc
    22801 agcttgcact tcacacattg ctcttgcaat agccattctc acagcttctg cttgtcctgt
    22861 tgaaccacca ccttgtacat ttactttaac gtcaaagttg gttgcatttt ctgtcataga
    22921 catcggttgc attactttgt actgtaaagt agcagtaggg aagtagtttt caaattctct
    22981 tttgtttacg gtgatttttc ctgttccttc tgaaacataa acacgtgcca cagcacattt
    23041 tcttctaccg attttgtgaa taactcccat tactttagat cgtttaagtt aacggttttt
    23101 ggttgttgag ctccatgttt gtgctcaata ccaacattta catttaaatt tcggaataat
    23161 tctgcaccta atttgttttt aggtaacatt ccttttacag ctttctctac taataatgca
    23221 ggattttttt gttgcattac tttagcggtc aagcttcttt gtccacctgg gtaacctgtg
    23281 tgacggatgt acgttttgtc atccaacttg ttaccagtca ggttgatttt ttctgcgttg
    23341 ataacaatta cgttatcacc acaatcaacg tgcggggtat aattaggctt gtatttacct
    23401 ctcaatagca tagctacttt tgaagcgaaa cgtcctaagt tatgaccctc agcatctaca
    23461 acaatccact gcttgtctgc ggtagctttg ttggctgaaa ctgtcttgta gcttaatgtg
    23521 ttcacaataa tttattttaa ttaaacattc catccccaat aaaggggttg caaaagtaca
    23581 attatttatt ataaaagcaa atagctattt gagaatattt tttttatcac ttttttgagc
    23641 ctattttttt cttaaatcta tttattttag actcttaaca gcctatttaa aatttttaat
    23701 tggcagttga taaaagcttt tttttagttg ataatctaaa aaaaaagaat aataagcttc
    23761 agatatagtt aaaaccctta tttttctatt cattatttta ctatttttac gtctaaattc
    23821 aaaaggtatg aagccaaaag ccacattaaa gcaaatttca aaagatttag gaatttctgt
    23881 ttccaccgtt tctaaagcgt tgagcgatag tcatgaaatt agtgaaccaa ctaaaattag
    23941 agttcaagaa tatgctaagt tgcaaaatta taagccaaac actatggcta agaatttgaa
    24001 aaatcaacgt acgaatacga ttggcgtaat cattccaaat attttgaatc ctttttttgc
    24061 aaaagtattt agtggtattg aaaaagcggc taatgcaaaa ggatacaatg tgattacgta
    24121 tatttcaaat gaatcacatg ccaaagaaaa acaagcaatg gatttgttaa gcaatggaac
    24181 tgttgatggt tttatacttt ctgtcgctca ggaaacgcaa tcgctacatg aaacgcaaca
    24241 ttttaaagat attttacggg aaggaatgcc catcgttatg tttgaccgaa caatggaaga
    24301 cgtttcttgt gataaagtta tagtagatga ttttgaatca tctgttgaag cagtcaatca
    24361 tttaatgaaa ttagggtgta ataaaattgc tttactttcg tgtattgata atttaagcgt
    24421 tggaaaacac cgtgctttgg gatatgaacg agcattgcag gatcataaaa gaagtgttga
    24481 agattactta gtaattaaaa cggatagtga agaagatttt gatgcaagat tgaaaaaatt
    24541 attgtcgagt ggtgaagttg atggtatttt agctgttgac gaacatgcct ctaccatggc
    24601 aatgaaagta gcaattcaaa aaggaataaa aattccggaa gagctttgta ttattggttt
    24661 tgccgatggt gtttggtcaa gaagattgac gccaagtttg tctaccatta gccaacatgg
    24721 tcctgaaatt ggtgaagaag ccgcgaaatt attaatagaa aaattagaac gcaaacaaga
    24781 aatacctacg tttaaaacta ctgttgttaa aacggaattg agacaacggg actctacaag
    24841 gagggtttag tttatatttg actggaattc taaaaactcg tttttatact atacacaacg
    24901 ctgtctaagg tttaactttg acagtgtttt ttatagttta ctaaaaactt tcttaatgag
    24961 cctctaacca atttctaccc attcctaatt ccacatccaa cggaacagtt aatttgaaag
    25021 cgttttccat ttcgtgttta atcatcggtt gaattttttc aagttcagaa ttgtgaacgt
    25081 cgaataccaa ttcgtcatgt acttgcaata acattttgga ttgccatttt tcggaagtca
    25141 atttttgatg aatattgatc atcgcaattt taataatatc ggcggcagaa ccttgaatgg
    25201 gggcgtttac tgcatttcgt tcagcaccgc ttcgcaccat cgcattcgca gaattgatat
    25261 cttttaaata acgtcgtctt cccaaaaccg tttgtacata accatgttct cgggcaaaat
    25321 cgacttggtt tgccatatag gctttcaatt ttggataagt ggcataatag gcatcaatca
    25381 actcggcact ttcttttcgg gataaattgg tttgagtgga aagtccgaat gcggaaacac
    25441 cataaataat tccgaagttc accgttttgg cattacttcg ttgttctttg gtgacttctt
    25501 ccagcggaac attgaacact tttgcagcgg tacttttgtg aatatcttca ttattttgaa
    25561 acgcttttat catattttct tcgccggata aagcggcaat aatacgaagt tctatttgtg
    25621 aataatcggc agaaactaac gtataatttt catctctggc aataaatgct tttcggatta
    25681 atcgacctct ttcggtacga ataggaatgt tttgcaagtt cgggttgttt gaactcaatc
    25741 gcccggttgc agcaaccgtt tgcatataat cggtatgcac acgttgcgtt tttggatcta
    25801 cttgattggg caacgcatca atatacgtgc tttgcaattt caccatttgt cgccattcta
    25861 atatgtcttt cacaatttgg tgttcgttca ccaaataact caagacctct tcgccggtgg
    25921 catactgacc ggttttggtt ttcttttgtt tggctccgcc gatttttaat ttatcaaata
    25981 aaatatcgcc caattgcttt ggagaagcca aattgaattt ttcaccggct gtttcataga
    26041 ttttttgttc aaagtcagtg atgtcttttt gcatctccac tgacattttt ttcagaaaat
    26101 caacatctaa tcgaatgcct tcggattcca tagcggctaa aaccggaatt aaaggaattt
    26161 caatttcatc aaacaatttt ttcgtttcgg ctttgtccaa aatcggacta aaaacttctt
    26221 ttagttggaa ggtaatgtca gcatcttcag cagcatattc tttgatgtct tccaaattga
    26281 catcacgcat tgatttttga tttttacctt ttttaccaat caaggtttca atggattttg
    26341 gcgaatattt caaatatgtt tccgataaaa tatccatatt gtgacgcata tcgggattga
    26401 tcaaataatg agcaatcatg gtatcgaata attttccttt gacagaaatg ccataatttg
    26461 ctagtatttt taaatcatat tttatgttct gaccgatttt ttcaatggtt tcactttcaa
    26521 aaaaaggtct gaacttttca attagttgtt gagattcatt tttgttttca ggaaacggaa
    26581 cgtaaaatgc ttttcctttt tcccacgaaa aagataaacc aaccaattct gcatttaatg
    26641 catcaattgc tgtagtttca gtgtcaaaac aaaccgaagt ttgattcatc aaattctgca
    26701 gaagcaattt cacaggcaaa tcaccttgaa tactttggta aaaatgttcg gtagtttcta
    26761 aagtagcata atatgaatta ggtttcgctt cat
//
LOCUS       DAOTCW010000015        26004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_954_length_26004_cov_53.218274, whole genome shotgun sequence.
ACCESSION   DAOTCW010000015 DAOTCW010000000
VERSION     DAOTCW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 26004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26004
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_954_length_26004_cov_53.218274"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="P5335_02300"
     CDS             complement(<1..148)
                     /locus_tag="P5335_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRZ73743.1"
                     /translation="MIKVSDTAKKKIIDLMQDDGFDAARDYVRVGVKSGGCSGLSYEL
                     KFDKE"
     gene            322..1182
                     /locus_tag="P5335_02305"
     CDS             322..1182
                     /locus_tag="P5335_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRZ73744.1"
                     /translation="MKLYPIEAGNFKLDGGAMFGVVPKTIWNRTNPADENNLIDIAAR
                     CLLIEDGNRLTLIDTGMGNKQSDKFFGYYSLWGEHSLDKSLANFGFHRDEVTDVFMTH
                     LHFDHCGGSVNWNSAKTGYEVAFKNAKFWTNENHWEWATKPNSREKASFLYENIIPMQ
                     ESGQLNFIKRPNADFGFSEELNFGIFYADGHTEKQMLPHIKYQDKTIVFCADLLATAG
                     HIPIPYVMGYDTRPLLTLDEKAKFMNAAAENNYYLFLEHDAHNEIITVEKTEKGVRLK
                     EVFKWEDVLR"
     gene            1240..2841
                     /locus_tag="P5335_02310"
     CDS             1240..2841
                     /locus_tag="P5335_02310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRZ73745.1"
                     /translation="MNFIKSFSIIVVSGLILTGCGPTKSVSFTQINPINAETVPLKNG
                     KISDAELLRWSHLDIVKDTIPGMSVDRAYNELLKNKTGQKVIVAVIDSGSDIEHEDLK
                     GRVWTNAKEIEGNGIDDDKNGYVDDVHGWNFLGDVIHENLELTRILKKGDDGSEIFKK
                     AQKKMDEKKQEALGNKQQLDFILAADKGIAEHLKKENYTLEEVKSILTTNPTLGNYKM
                     VMMQVLATTPREEFVKELDEFKDYVYDQLNYNLNLEYDGRKILGDNAEDISNTSYGNN
                     QVWGPNKKYAMHGTHVAGIIAQVRGNNLGGDGVATNVEIMAVRAVPNGDEYDKDIALA
                     IRYAADNGAKVINGSFGKGFSPHKEWVYDAIKYAASKDVLFVHAAGNDAEDLNVVESY
                     PNDRIVETEIVTNYIKVGALNYEYGSKLVADFSNYGKTDVDVFAPGVKIYATIPNNLF
                     KFEQGTSMAAPNVAGVAALIRSYYPKLTAAQVKQILKDSGVAISKTVIVGGDASNKIS
                     FNELSVTGKIVNAYNALLMADKMSK"
     gene            2847..4730
                     /locus_tag="P5335_02315"
     CDS             2847..4730
                     /locus_tag="P5335_02315"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRZ73746.1"
                     /translation="MKKIAFLITLSFANILSAQNNPNPGYWQQQADYKMEVEMDVKSF
                     QYTGNQILEYTNNSNDTLTKVFFHLYPNAFQPGSEMDIRLQTIKDPDKRMVKSFKVAD
                     KEVKESRISTLKPEQIGYLKISNFKQDGQVATTKVVGTILEVTLAKAILPKSKTVFSL
                     NFDGQVPEQIRRSGRNSSEGVALSMTQWYPKICEFDFEGWHTDPYIGREFHGVWGNYD
                     VKITIDKNYTLGASGYLQNKNEIGHGYQDEGIEVKHAKKTKTLTWHFVAPMVHDFAWG
                     ADNNFLHDKIIGENGVELHFLYKNDKEYVENWKAMQPKTAELLSFFNKNIGPYPYKQY
                     SVIQGGDGGMEYAMCTLITGKRSVGSLVGVTAHEMAHSWFQHVLATNESKHEWMDEGF
                     TSFISDLAMTEVMPSKNKKDDENPFLSSYNNYFYMVTTGKEQPQSTHADRYDENMVYG
                     ISSYSKGTVFLTQLGYVIGIENMMKTLKRFYYDYQFTHPTPNDFKRTAERVSGAVLDW
                     YLVDWTQTTNTIDYGIKNIQDDGKVVLERIGRMPMPLDILVTYEDGTVESFYIPNPLM
                     RWEKENPYPQIKRTVLKGWDWAYPTYELPLSQEKSKIKSIAIDPSGLMADVNKENNVF
                     EKK"
     gene            4811..7048
                     /locus_tag="P5335_02320"
     CDS             4811..7048
                     /locus_tag="P5335_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRZ73747.1"
                     /translation="MKLKLFVLFGLLVGLISCNKSNEYVAIVDLHNAKAAFVGNQTCV
                     GCHQKEVKDWEHSHHAKAMMEANETNVLGDFNSVQLVRNGLTHKMFKKNDAFYVITDG
                     PNGTMQEFQVKYTFGYHPIQQYLVEFEQGRLQVLSLTWDVVKKEWYYMSDEVYKNLSI
                     DHGNWLHWTNQAYNWNSMCAECHTTNLKKNYNPETDSYKTTWSQLSVNCEACHGPAEF
                     HLKWTADKEMDIANYGFVKKFKNSDNKSQIETCVRCHSRRSIYGDYDYNWEHTHEQIA
                     IATVGFPNYHSDGQIKEEDFEIGSFSQSKMHEKGVKCTDCHNVHSGKVKFQDNRLCTQ
                     CHLPTQYDSEKHHHHDKNKPGASCVDCHMPGQYFMGRHFRRDHSLRIPRPDISKVTNS
                     PNACNACHKDKSVDWAVEKTTLWYGTKTKDHYGFTFHKAGLQDPSAIDELKIIINDEQ
                     KPIIVRQTAMDVLRLNYPEQSQPILNPFLTNKKIALRYQSVFNSKIDNTNSSQLLALL
                     KDPIKGIRSTAAIKIAENKSFLTAAYQSAYDKAIKEYLAILTYNSDFPDAKLNLGNYY
                     LSQNDSKKAENYYLKTINQDKELTQAYYNLAYLYNNTNKKSEAITVLETYLQNNPKNS
                     YAYFDIGLLIAEYGDYKKAVVYLEKAKNNVTPNDRIILNLAKIYDYLGNKKQAENYFN
                     QLVQNFPLIQEYYVELFQFYIKNKNTIKAKQTAVKILQKFPDFAERDLLENFINSEFP
                     TNSNQ"
     gene            7125..7502
                     /gene="rnpA"
                     /locus_tag="P5335_02325"
     CDS             7125..7502
                     /gene="rnpA"
                     /locus_tag="P5335_02325"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRZ73748.1"
                     /translation="MSFSYPKHEKLKSQKQLDLLFSEGKTVSKYPLRLVYVPVELEDE
                     EKIKFGVSVSKKHFKLAVDRNYYKRLLRECYRLNKHLLLENLDKPYAIMFFYQTKEAM
                     PFEEMNEKTVLLFEKFIKEIKST"
     gene            7643..>8701
                     /locus_tag="P5335_02330"
     CDS             7643..>8701
                     /locus_tag="P5335_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRZ73749.1"
                     /translation="MLSRFKKRYIIPVILSGFLFIGVGFKEDFFEIAKQIEIFTNLFK
                     TVNMNYVDETNPGEMMDKAIKSMLADLDPYTNYFNEADVVKFKINNTGEYTGIGAMIT
                     RKEGKIIIKEPYKNYPADKAGLKAGDEIIQIGDVELKDYKEDASALMRGAKNAKVEVK
                     FIRQGKMNTATLILDEVEVKAVPFYGKVDEKTGYIVLSQFNRKATFETQAALLDLKTQ
                     GAEKIILDLRGNPGGLLGEAVNICNLFVPKGEIIVTTKSKIEKHNQTYKTTRDPIDLD
                     VPLVILVDGKSASASEIVSGALQDLDRAVVVGSRSFGKGLVQRPLDLTYGTQVKVTIS
                     RYYTPSGRCIQALDYSKKD"
     assembly_gap    8702..8801
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8802..9157
                     /locus_tag="P5335_02335"
     CDS             <8802..9157
                     /locus_tag="P5335_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HRZ73750.1"
                     /translation="SDADFTDFKSFLKKENFSFDTETEKQLKKTLETAQKEKIEENIK
                     VEYQNLMTALRKSEEIQLNKNQTEIKKLILDELIKRYQYKEGLYQYYIQNNSEVKKAT
                     SILANQTEYNKILKK"
     gene            9154..9978
                     /locus_tag="P5335_02340"
     CDS             9154..9978
                     /locus_tag="P5335_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRZ73751.1"
                     /translation="MIKHGFAFFLLFISISLFAQEEMVQSIYFDTNKSDVDLKQTTQM
                     VDFIKKIDSTRIESIQIFGYCDDVGKDDYNRKLSTERANKVKSKIIDNGITNKIIVTI
                     EGKGRILIEDDIVDNLPAVRSKNRRVDVVLNLKPLPKEDLKIPGVYPTFTKDNIVGDR
                     IVLSDMLFERGSSVLTVKCKNELDKMAKLLNKYKNLHIEIQGHVCCTPPNHKEAIDKA
                     TKKRKLSQNRAESVYKYLISKKIAKDRLTFKGYGNTVSLGKSPELDRRVELVITKL"
     gene            complement(9975..10421)
                     /locus_tag="P5335_02345"
     CDS             complement(9975..10421)
                     /locus_tag="P5335_02345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRZ73752.1"
                     /translation="MQLTFKIKSFNELSLTELYNVLQLRSEVFVVEQNCVYQDIDGKD
                     EKAIHLIGTFNNEIVAYVRIFIPGDYFEAASIGRVVVKQSYRDRKWGYDLMQEAIEVT
                     KTVLNETYITISAQLYLKKFYENLGFVQTSEMYLEDDIPHIEMKKS"
     gene            complement(10452..11423)
                     /locus_tag="P5335_02350"
     CDS             complement(10452..11423)
                     /locus_tag="P5335_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HRZ73753.1"
                     /translation="MKNIFILLFCVTFIVSCKEEKSGTAYKESSGKINHLSVIIDNQL
                     WNGEVGDSIRKKFAAPVDGLPQEEPLFTIKQYAPNLFEGFMTNSRNILVVLKKENNAF
                     ELKENEFAQPQNVAHISGGSVSEIIKLLETHSQEIIKKFKKTEIAENQKRIAKSLLND
                     TKIKTKFKASLKLPTAYRYIIEEEKFFWIRKELISGNASLLIYEVPLSTIQKDSNTMT
                     NIIQMRDSIGQKHIQGTLPNTFMVTEAAYSPYFFYTILDGKKTYETKGTWELKNDFMA
                     GPFINYAIKDEKNNRYLIIEGFCYNPSQSKRDLMHELESIIKSIKLL"
     gene            complement(11453..>12507)
                     /locus_tag="P5335_02355"
     CDS             complement(11453..>12507)
                     /locus_tag="P5335_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRZ73754.1"
                     /translation="VNHFETDTIMVKNKMTFKQLSDLLSISVEQLRFLNPQYKRDYVP
                     YMADKKHFVRLPIDKVAVFTSNESKIYAYTEYDFNKREKPFTSNQAYVSEGMVSKTKY
                     HKVRKGESLGGISKKYGVTISELKKWNKLKSNKVATGKSLKIITDEAVAVAPKKTSDK
                     SVAISNDTIKSKSEKEKDSTKFHLVERGENLYSIAQKYSVTKEDLKEWNNLKDENIQL
                     GSKLLVSAAINQEKTENYVVQSGDNLSTIAKKYGVGIDDIKKWNNLEDYNVKLGSTLK
                     IIVPEKEEIVAEVKTQKPEKHSTFNKEDIYIVKKGDSLFSISKKIKGVTVSDLKKWNG
                     ISGGNIKPGMKLKISG"
     assembly_gap    12508..12607
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12608..13185)
                     /locus_tag="P5335_02360"
     CDS             complement(<12608..13185)
                     /locus_tag="P5335_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRZ73755.1"
                     /translation="MNLRNTTLATVLLFSTLFHAQDTTIVSINQKVEPKISYLDSIKQ
                     SFVKHEVASCVDERWMSELSNQDLFNEMIADIQNVNPENNVDYDLSTDLLKERLKILD
                     SKSAFHIEYNKGLENIIKHYLKNRKKAYERLMSLSQYYFPIFEESLAKYNVPLEIKYL
                     AVVESALNPLAVSKAGATGLWQFMYGTGKQYNL"
     gene            complement(13285..14472)
                     /locus_tag="P5335_02365"
     CDS             complement(13285..14472)
                     /locus_tag="P5335_02365"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRZ73756.1"
                     /translation="MKTLFDFNFNNKKALIRVDFNVPLDEKFNVTDANRIEAAKPTID
                     KILADGGSVILMSHLGRPKGVQDEFSLRHIVVKISEVLGVPVQFASDCIGEIAEKAAN
                     NLKAGEVLLLENLRFYKEEEAGDLEFSKKLASLGDIYVNDAFGTAHRAHASTTIIAQF
                     FPNHKCFGMLLAKEIESLNKVLKNSVKPVTAVLGGSKVSSKITVIENILDKVDHMIIG
                     GGMTFTFIKALGGKVGNSICEDDKLQLALDILHMAKEKNVHIHIPVDVVAADAFSNDA
                     NTQIVDVREIPDGWQGLDAGPKSLENFKAEIMNSKTILWNGPLGVFEMEAFAHGTIAL
                     GNYIAESTANGAFSLVGGGDSVAAVKQFGLEDKMSYVSTGGGAMLEMLEGRTLPGIAA
                     ILD"
     gene            complement(14560..16434)
                     /locus_tag="P5335_02370"
     CDS             complement(14560..16434)
                     /locus_tag="P5335_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRZ73757.1"
                     /translation="MNLKRCKNALFIVFLIFSSWSNAQLTVPFKERYKVNVKGDMTII
                     ANSVLNRIDKKNSTNMPFNDKTFETLSNDEMDMEYIDIDTDKETFSSSSAALFLKNEE
                     SKKIIYAGLYWSATYKYESGYKKGEDKFVAFDKTREFFDEVLVKFPTQKEYKSIKGEI
                     IFDGIKDKNLKEVAPYTVYADITPYVKELENPFGFYTVANIKATQGTLIGGSAAGWTI
                     IFVYEDLSMTEKYISSYDGFAGASSERVDINFKNFETQPEGDVIVKLACSALEGDFNV
                     DGDLLMFSSSTNTSLSNIKTGTRKYSNFFDSSITIEDENFQYRIPDSKNTLGYDALIT
                     TLRNPNNSKIGNNAKDARVSFKSSGDVFYMFFCALNIEIKPSKIQIEQPQDNTKVVFE
                     DKKFKLVDKTTNKEIIQEPSPQTIAENAFKPKVAETETSLKPEVKAVTKSKDKSTKPN
                     ATSVPSTTDIVANTNSKPKEKEVAKKSPEAKVVTSSGTINSEKSENLSAQSSEKQKPV
                     DSKLVSTTELKKEDKKSNSLENSVNNSVQRLNNIDMPKGYYLVANVFNERVNAAKFMM
                     KLRDRGLDPNYFTSPENNFMYVYLDFVNTRAEADALRESKLKNTYFEELWILIVNITE
                     "
     gene            16662..17810
                     /locus_tag="P5335_02375"
     CDS             16662..17810
                     /locus_tag="P5335_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRZ73758.1"
                     /translation="MQFSEILGQEHIKNHLTQSVQLGRIPHAQLFVGPEGSGTLPMAI
                     AYAQYLLCSNNGAENSGGNESCNLKFLHFAHPDLHFIYPTVTTDEVKSKPKSIDFIQE
                     WREFLNQNTYGGLFDWFKILDVKNKQGEIRVDDAQEIVRSLSLKSYEGGYKVMIIWMA
                     DKMNIEASNKLLKILEEPTDRTVFILITENEEDLIQTIRSRCQVLDFNGLPEQVIAEA
                     LVKYKKLDQNTASKIARQAQGNYNKALKLISEDNEDLPFEQWFVIWVRAAFKAKGNAA
                     AIHELIEWSEEIAKMGRETQKKFLTYCLEMFRQALLLNYQATSLVYLEPKVEKFKLEN
                     FAPFVNGNNIINIYKELNDAIYHIERNGNAKIILTDLSIKLTRLIHKK"
     gene            17815..>17999
                     /locus_tag="P5335_02380"
     CDS             17815..>17999
                     /locus_tag="P5335_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRZ73759.1"
                     /translation="MNNPASFLILLFLAITFLQSGYDKLFYWNDNLGWLKGHFSKTIL
                     KNMVPLALAKILILELIS"
     assembly_gap    18000..18099
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18100..19766)
                     /locus_tag="P5335_02385"
     CDS             complement(<18100..19766)
                     /locus_tag="P5335_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIF1 family DEAD/DEAH box helicase"
                     /protein_id="HRZ73760.1"
                     /translation="MEITNDARQVLQFINQTQRSVFLTGKAGTGKTTLLRQIIQTTHK
                     NTVVVAPTGIAALNAGGVTIHSMFQLPFSGFIPEYIPGSSISDYQKFETKNTLVKHFR
                     MSGQKKSVMLNMELLIIDEVSMLRADLLDAIDFTLRKIRRKDFPFGGVQVLFIGDLLQ
                     LPPVVKNEEWRVLRNYYRGIFFFNAKVIQESPPLYIELTKIFRQSDERFISVLNNLRN
                     NMISSQDMMILNDFVNPKFDIKSNKGYITLTTHNAKADEINAASLDEIKEKCFRFKPE
                     IVGDFPEKIYPVEEILQLKVGAQIMFIKNDLSPEKNYFNGKMGFVKSLSEGEIIIHFP
                     EENKIIEVEKYEWKNIRYSVNENTKEIDEEVLGSFVHFPIKLAWAITVHKSQGLTFEK
                     AVLDVSQVFLPGQAYVALSRLRSLDGLILLKPIQMNGISNDQEVMQYAENKADNETIV
                     KTLQKETQRFIHEYIKNSFDWTELAQDWRNHRFSYLANAEKSEKSKHQKWAEIQENAI
                     SELEEPAKKFRSQLNRLFQVEEVDILFISERIKAAFDYFFPKTDSVL"
     gene            complement(19888..20463)
                     /locus_tag="P5335_02390"
     CDS             complement(19888..20463)
                     /locus_tag="P5335_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRZ73761.1"
                     /translation="MKKSILVFGLILALASCEKAAEQKDFKTAYVDTSNLLEEYTEAK
                     DIEAKYKSKGEEMGKQLEAEVARFRSEASSFQKNAQTYGPQWAQQKGAELQKKEQELS
                     YAQQAILRQLQEESGKEMDSLVKDVKKFIKDYGKEKGYNYIYGTGDAASVLYAKEEYD
                     ITKEIIKLLNDKYKAKSSTETKKEDTAEAKK"
     gene            complement(20700..>20858)
                     /locus_tag="P5335_02395"
     CDS             complement(20700..>20858)
                     /locus_tag="P5335_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRZ73762.1"
                     /translation="MWFDSFYVSLLSEKYKTGKMNLFSAFWVGLQSNLKGRQTKEYSS
                     HIYIFKNS"
     assembly_gap    20859..20958
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20959..21398)
                     /locus_tag="P5335_02400"
     CDS             complement(<20959..21398)
                     /locus_tag="P5335_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRZ73763.1"
                     /translation="MNFLKQAYIKVKDHSVSGEEFELLLDEDLQLLKTYPQPSLELLP
                     NYYESEDYISHTDGQRTLFEKVYQFIKKKALNDKVKLIEYWHTGKGTVLDIGAGTGDF
                     LLVAKSRGWKAVGMEPNSKAKTISKKKGICIVDNYSELEDHFFDV"
     gene            complement(21522..23393)
                     /gene="mnmG"
                     /locus_tag="P5335_02405"
     CDS             complement(21522..23393)
                     /gene="mnmG"
                     /locus_tag="P5335_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRZ73764.1"
                     /translation="MFQEKYDVIVVGAGHAGSEAAAAAANMGCTTLLVTMNLQTIGQM
                     SCNPAMGGIAKGQIVREIDALGGYSGIVSDKTAIQFKMLNKSKGPAMWSPRVQSDRMR
                     FAEEWRLMLENTPNVDFYQEMVAGLIIENNVIKGIRTSLGIEIKAKTVVLTNGTFLNG
                     LIHIGEKQFGGGRAGESAAYGITEDLIKVGFESGRMKTGTPPRVDGRSLDYSKMREER
                     GDEKPHKFSYLDSSKPLVEQKSCWMTYTSLDVHAILREGFDRSPMFNGRIQSTGPRYC
                     PSIEDKINRFADKDRHQLFIEPEGWNTVEVYVNGFSTSLPEDVQFKALRSVEGFEKVK
                     FFRPGYAIEYDYFPPTQLKHTLETKLIEGLFFAGQINGTTGYEEAASQGLMAGINAAL
                     KVQEREPFILKRDEAYIGVLIDDLITKGTEEPYRMFTSRAEYRTLLRQDNADERLTPK
                     GFALGLASQERLIRMEYKFKEAEKMVNFFRETSITQAEANPVLEEKGSALMVQSDKMY
                     KVFSRPQIELEDIVKFQKVKEYISTNQLDDETVEQAEIQVKYNGYIEKERNNADKLKR
                     LEDVKIPENYDFDKIKSLSYEAREKFKKIRPVTVSQASRISGVSPSDISILLIYMGR"
     gene            complement(23448..23870)
                     /gene="ybeY"
                     /locus_tag="P5335_02410"
     CDS             complement(23448..23870)
                     /gene="ybeY"
                     /locus_tag="P5335_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRZ73765.1"
                     /translation="MISFNYETDFELGNETLFSNWIAKIIESENRLEGEINYIFCDDD
                     YLHKINLQYLNHDTLTDIISFDYSEGNFLQGDIFISIERVQDNATDFNISLLEELKRV
                     MAHGILHYCGYKDKSAEEEKIMRQKEEEKMKMFHVEQS"
     gene            complement(23863..>26004)
                     /locus_tag="P5335_02415"
     CDS             complement(23863..>26004)
                     /locus_tag="P5335_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73766.1"
                     /translation="VYYPQNNDKAALKSVLPVKKDVFDQFIFTFPGNLPVEEGVSYEY
                     YFEVFDNDALHNFKSSKSSTFSNRINTEEERQDEQLKQQNDNINSLQKSLKNQDKQLS
                     ELDKIQKMGKEKNNLEFKDKQKINDFIKRQKQQEEMMKEFTKKLQEDLKNAKSDNKDE
                     FKKELEKRAENAEKEADKNQKLLDELEKLAEKLNDVEMFDKMDKIKQSTKNQTKNLEQ
                     LVELTKKYYVEKKAEQLASKLDKLAEKQDNLAKQEKENTTEKQNDINKEFEKIQEELR
                     ELEKDNKELKSPVDIPSDEKQEKSIKEDLQKASDELQKQQKEKAKPKQKSASQKMKQM
                     SQQMMESMAGGEMEQLEEDVKMMRQILDNLLAFSFSQEDLMKQFKSLKKGAPSYNKYL
                     KQQQDLKQQFKHVDDSLFAMSLRNAMITEKVTEEIGNVQYNVDKSLESLAEAIISKGV
                     SHQQYTVAASNRLADFLSDVLNSMQMSMSGAGAGKPKPGKGQGGMQLPDIIQKQKGLS
                     DKMKEGMEKGDKPGDKPGESPGEKPGDGKKGDKGDKPGGKDGEKGKGNQKGNKPGEGS
                     GSGGQNNSDGEENARELMEIYQEQRRLREQLQDALEKQGLTPSGQKVLDQMKDAEKQM
                     LNKGFKNEVLQRMLNINYEMLKLEKALQQQGEDTKRQSETNKKEYNNAVKPLSPELQQ
                     YLNSVEILNRQSLPLRPNYNQKVQEYFKKND"
BASE COUNT         8263 a         4366 c         4082 g         8893 t
ORIGIN      
        1 tttctttgtc aaattttaac tcataagaca aaccggaaca accaccgctt tttacgccta
       61 cacgaacgta atctcgggcg gcatcaaaac catcatcttg catcaaatcg atgattttct
      121 ttttagctgt atctgaaact tttatcattt gctattttga ttttgtctaa ataaagagca
      181 aatatacacc aaaacaagaa aattaaaaca atttctaaca tttttataac taatgttttg
      241 atagaaaata gttataatag aacttgtaaa acgtatgttt ttagtaattt gcgaaacatt
      301 tttatcaata cttaaaaaat tatgaaatta tatccgattg aagcagggaa ttttaaactt
      361 gatgggggag ccatgtttgg ggtagtaccc aaaaccattt ggaaccgaac gaatccggct
      421 gatgaaaaca acctcatcga cattgcagcc cgttgtttgt taattgaaga cggaaatcga
      481 ctgactttga ttgacaccgg aatgggcaac aaacaatcgg ataaattttt tggatattac
      541 tctctttggg gcgaacattc actagataaa tccttagcaa attttggatt tcatcgtgac
      601 gaagtgacgg atgtttttat gacgcatttg cattttgacc attgtggtgg cagtgtaaat
      661 tggaactcag caaaaaccgg atatgaagta gcatttaaaa atgcgaaatt ttggaccaat
      721 gaaaaccatt gggaatgggc aacaaaaccc aattcgcgtg aaaaagcctc ttttttatat
      781 gaaaatatca ttccaatgca agaaagcggt cagttgaact ttatcaaaag accaaatgct
      841 gattttggtt tttcagaaga attgaatttt ggcatttttt acgcagatgg tcataccgaa
      901 aaacaaatgc ttccgcacat caaataccaa gacaaaacga ttgttttttg tgcggattta
      961 ttggcaactg ccggtcatat tccgataccg tatgtgatgg gttatgatac cagaccactt
     1021 ttgaccttgg atgaaaaagc aaaattcatg aacgctgctg ctgaaaataa ctactattta
     1081 tttttagaac acgatgctca caacgaaatc attaccgttg aaaagacaga aaaaggcgtt
     1141 cgtctaaaag aggttttcaa atgggaagat gtcttaagat agtgttaatc tcgtttttat
     1201 ccttttataa aattatattt ttaccccaaa ttttttatca tgaatttcat taaatctttt
     1261 tctattatag tcgtttccgg cttaatttta accggttgtg gacctacaaa atctgtttct
     1321 tttactcaaa tcaatccaat aaatgcagaa actgttccgt tgaaaaatgg taaaatttct
     1381 gatgccgaac tattgcgttg gagtcattta gacattgtta aagacactat tccgggcatg
     1441 agtgttgacc gtgcttacaa tgaactgtta aaaaacaaaa ccggtcaaaa agtaattgtt
     1501 gcagtaatcg acagtggttc ggatattgaa cacgaggatt taaaaggtcg tgtttggact
     1561 aatgctaaag aaattgaagg caatggaata gatgatgaca aaaacgggta tgttgatgat
     1621 gttcacggtt ggaatttttt aggtgatgtg attcatgaaa acctggaatt gaccagaatt
     1681 ttgaaaaaag gagatgacgg ttctgaaata ttcaaaaaag ctcagaaaaa aatggatgaa
     1741 aaaaaacaag aagctctcgg aaacaaacaa caattagatt ttattctagc tgcagacaaa
     1801 ggaatagcgg aacatttaaa aaaagaaaat tatacactag aagaggtaaa atccatttta
     1861 acaactaacc caacgttagg aaattataaa atggttatga tgcaagtttt agcaactact
     1921 ccgcgtgagg aatttgtaaa agaattagat gaatttaaag attacgtcta tgatcaattg
     1981 aattacaact tgaacttgga gtatgatggg cgaaaaatat taggtgataa tgcggaggat
     2041 atttctaata catcctacgg caacaaccaa gtttggggac caaataaaaa atatgccatg
     2101 catggaactc atgtagccgg aatcattgct caagtgagag gaaacaattt agggggagac
     2161 ggagtggcta ccaatgttga aataatggca gttcgtgctg ttccaaatgg tgatgaatat
     2221 gataaagata ttgccttagc aattcgatat gccgcggata atggagctaa agttattaat
     2281 ggtagttttg gaaaaggatt ttctccacac aaagaatggg tttatgacgc tattaaatat
     2341 gctgcaagta aagatgtact atttgttcat gctgccggaa atgatgctga agatctgaat
     2401 gtggtagaaa gttatccaaa cgatcgcatt gttgaaacag aaattgttac aaattatatc
     2461 aaagttggag ccttaaatta tgaatatggt tcaaaattag ttgctgattt ttcaaactat
     2521 ggaaaaaccg atgttgatgt ttttgctcct ggagtaaaaa tttacgctac aataccaaat
     2581 aatctattca aatttgaaca aggaacatcc atggcggcac caaatgttgc aggtgtggct
     2641 gctttaattc gttcatatta cccaaaatta acagctgctc aagtaaaaca aatattgaaa
     2701 gatagtggtg ttgctatttc taaaacagtt attgtgggtg gagatgcttc taataaaatt
     2761 tcttttaacg aattatctgt aaccggaaaa attgtgaatg cctacaatgc cctattaatg
     2821 gctgataaaa tgtcaaaata aattctatga aaaaaattgc attcttaata acattatctt
     2881 ttgctaacat tctttctgca caaaacaatc caaatccggg ttattggcaa caacaggctg
     2941 attataaaat ggaagtggag atggatgtta aatcttttca atataccgga aatcaaattt
     3001 tagaatacac caataattca aatgacactt tgactaaagt attttttcat ttgtatccca
     3061 atgcttttca acccggaagt gaaatggaca ttcgactaca aaccatcaaa gatcctgata
     3121 aacgaatggt taaatcattt aaagtagcgg ataaagaagt aaaagaaagt cggatttcaa
     3181 ctttaaaacc ggagcaaatt ggttatttaa aaatttctaa ttttaaacaa gatggtcaag
     3241 tggctacaac aaaagtagtc ggaaccattt tagaagtaac tttagctaaa gcgattttgc
     3301 ctaaatcaaa aactgtcttt tctttaaatt ttgacgggca agttccggaa cagattcgtc
     3361 gttccgggag aaatagctca gaaggcgtag cactttcgat gacgcaatgg tatcctaaaa
     3421 tctgtgaatt tgactttgaa ggttggcata ctgatcctta tattggaaga gaatttcatg
     3481 gtgtttgggg aaattatgat gtaaaaatta caattgataa aaattatact ctcggagctt
     3541 ccggctattt gcaaaacaaa aatgaaatag gtcacggcta tcaagacgaa ggaattgaag
     3601 tcaaacatgc taaaaaaacc aaaaccttaa cttggcattt tgtggctcct atggtgcacg
     3661 attttgcttg gggtgccgat aataattttt tacatgataa aattattgga gaaaatggtg
     3721 ttgaacttca ttttttatat aaaaacgata aagaatatgt tgaaaattgg aaagctatgc
     3781 aacccaaaac tgctgaattg ctttcttttt ttaataaaaa tataggacca tatccatata
     3841 aacaatattc tgttattcaa ggtggtgatg gtggtatgga atatgcgatg tgcaccttaa
     3901 tcaccggaaa acgttccgtt ggaagtttag ttggtgtaac agctcacgaa atggctcatt
     3961 cttggtttca acatgttttg gcaaccaacg aaagtaaaca tgaatggatg gatgaaggat
     4021 ttacttcttt catttcagat ttagcaatga ctgaagtaat gccttctaaa aacaaaaaag
     4081 acgatgaaaa tccgttttta agtagctaca acaattattt ttacatggtt actaccggca
     4141 aagaacagcc gcaatccaca catgctgacc gttacgacga aaacatggtt tatggtattt
     4201 catcttacag taaaggaacc gtttttttga cacaattagg ttatgtgatt ggtattgaaa
     4261 acatgatgaa aaccttaaaa cgattttatt atgattacca atttacccat ccgactccaa
     4321 atgattttaa acgcacagct gaacgcgttt caggtgctgt tttagattgg tatttggtgg
     4381 attggactca aactacgaac accattgact acggaattaa aaacattcaa gatgatggaa
     4441 aagttgtttt agaacgtatc ggaaggatgc ctatgccatt ggatatttta gtaacgtatg
     4501 aagatggtac tgtggaaagt ttttatattc ctaatcctct gatgcgttgg gaaaaagaaa
     4561 atccgtatcc acaaataaaa agaaccgttt taaaaggctg ggattgggct tatccaacct
     4621 atgaacttcc actttcacag gaaaaatcta aaattaaatc gattgcgatt gacccgagtg
     4681 gcttgatggc tgatgtgaat aaagagaata atgtatttga aaagaaatag gtattttaaa
     4741 ttccttcttt cttttaatta agaataagtt gtagatttga ttcttactcg ttttttatta
     4801 aattcatttc atgaaattaa agttgtttgt tctgtttggt ttattggtgg gtttaatttc
     4861 ctgcaataaa tcaaatgaat atgttgccat tgtagatttg cataacgcaa aagctgcatt
     4921 tgtgggcaac caaacttgcg ttggatgtca tcaaaaagaa gtgaaagact gggaacattc
     4981 acaccatgcc aaagcaatga tggaagccaa tgaaaccaat gttttaggtg atttcaacag
     5041 tgttcaatta gttcgaaatg gtctcacaca taaaatgttt aaaaaaaatg atgcgtttta
     5101 tgtcatcacc gatgggccaa acggaacaat gcaagaattt caggtaaaat acacttttgg
     5161 ctatcaccca attcaacaat acttggttga atttgaacaa ggcaggcttc aagtcctttc
     5221 gttaacctgg gatgttgtaa aaaaagaatg gtattacatg tctgatgaag tttataaaaa
     5281 tctaagtatt gatcatggta attggctcca ttggactaat caagcctata attggaacag
     5341 tatgtgtgct gaatgtcaca ccacaaatct caaaaaaaac tataatcccg aaactgattc
     5401 ctacaaaacc acttggagcc aattgagtgt gaattgcgag gcctgccatg gcccggcaga
     5461 atttcattta aaatggactg ctgataaaga aatggatatt gctaattatg gttttgttaa
     5521 aaaatttaaa aacagtgata ataagtcgca aatagaaacc tgtgttcgtt gccattcgcg
     5581 tagaagtatt tatggtgatt acgactacaa ttgggagcat acccatgaac aaatagctat
     5641 tgcgaccgtt ggcttcccaa attatcattc agatggtcaa attaaagaag aagattttga
     5701 aatcggttca tttagccaaa gcaaaatgca tgaaaaaggt gtgaaatgca ctgattgtca
     5761 taacgttcat tcagggaagg tgaaatttca agacaatcga ttgtgtacac aatgtcatct
     5821 tccaactcaa tatgacagtg aaaaacatca tcatcacgac aaaaataaac ccggtgcttc
     5881 ctgcgttgat tgtcatatgc ccggtcaata ttttatgggg agacatttca gacgagacca
     5941 cagtttacga attccaagac cggacatttc taaagttaca aattcaccta atgcttgtaa
     6001 tgcgtgtcac aaagataaat cagttgattg ggctgtagaa aaaacaactc tatggtatgg
     6061 aacaaaaaca aaagaccatt acggatttac attccacaaa gccgggttac aagatccgtc
     6121 tgcaatagat gaactgaaaa ttatcattaa tgatgagcaa aaaccaatca ttgtccgtca
     6181 aaccgccatg gatgttttac gattaaatta tcctgaacaa agccagccta ttttaaaccc
     6241 ttttcttact aataaaaaaa tagcattacg ctaccaaagt gtttttaatt caaaaataga
     6301 taatacaaat tcatcacaac tattggcttt attaaaagac ccaattaaag gaattcgttc
     6361 aactgccgcc ataaaaattg cagaaaacaa aagtttttta acggctgctt atcaatctgc
     6421 ttatgataaa gccatcaaag aatacttggc tatactgaca tataacagtg attttccgga
     6481 tgcaaaatta aatttgggaa attattattt aagtcaaaat gattccaaaa aagccgaaaa
     6541 ttattattta aaaaccatta accaagataa agaattaacg caagcctatt acaatttagc
     6601 ttatttatat aacaacacca ataaaaaaag tgaagctata accgttttag aaacctatct
     6661 tcaaaataat ccgaaaaaca gttatgctta ttttgatatt ggattgctaa tcgctgaata
     6721 tggtgattat aaaaaagccg ttgtttattt ggaaaaagca aaaaacaatg tcactccgaa
     6781 tgatagaatc attttaaatc ttgctaaaat ttatgattac ctagggaata aaaaacaagc
     6841 tgagaattat tttaatcaat tagttcaaaa tttcccgtta attcaagaat attatgttga
     6901 attatttcaa ttttatatta agaacaaaaa caccataaaa gcgaaacaaa cagcggtaaa
     6961 aattttacaa aaattccctg attttgcgga aagagattta ttagaaaact ttattaatag
     7021 tgaattcccg acgaattcta accagtaatt taattcctca aaactcattt ggcaaaattt
     7081 aattcgttct gattaccttt gcttttcaaa gtaatcgaat tcaaatgtca ttttcttatc
     7141 ccaaacacga aaaactcaaa agccaaaagc agctggattt acttttttct gaaggaaaaa
     7201 cggtgtcaaa atatccgttg cggttggttt acgttcctgt ggaattagaa gatgaggaaa
     7261 aaataaaatt tggtgtttcg gtttctaaaa aacattttaa acttgctgtc gacagaaact
     7321 attataaacg cttgcttcgt gaatgttatc gtttgaataa acatctttta ttggaaaatt
     7381 tagataaacc ttatgctatt atgtttttct accaaacaaa agaagccatg ccttttgagg
     7441 aaatgaatga aaaaaccgtt ttgctttttg aaaaatttat taaggaaata aaatccactt
     7501 aaagtttggc ttgttatttg tgctttcaat tatttgaaaa agggtttaac gtattatcaa
     7561 ctgtttatct caatttaatt cttaatttag ttgccctatt ctttctgtaa aaaatattaa
     7621 aaaaataata ccattatttc tcatgctttc aagatttaaa aaacgctata ttattccggt
     7681 tattctttcc ggatttttat ttataggagt cggattcaaa gaagattttt ttgaaattgc
     7741 taaacaaatc gaaatcttta ccaatttgtt taaaacggtc aacatgaact atgtagacga
     7801 aaccaatccg ggtgaaatga tggataaagc cattaaaagt atgctagccg atttagaccc
     7861 gtatactaat tatttcaacg aagctgatgt ggttaaattc aaaatcaaca acacgggtga
     7921 atacacggga attggtgcaa tgattacccg taaagaagga aaaatcatta ttaaagaacc
     7981 ttataaaaat tatcctgccg ataaagccgg ccttaaagcc ggggatgaaa ttattcaaat
     8041 tggcgacgta gaattaaaag attacaaaga agatgcttcg gcattaatgc gaggagcaaa
     8101 aaatgcaaaa gtggaggtaa aattcatccg acaaggcaaa atgaatacgg ccaccttaat
     8161 tttagatgaa gtagaagtaa aagcggtccc gttttatgga aaagttgatg aaaaaacggg
     8221 ttatatcgtg ctttctcaat ttaacagaaa agccactttt gaaacccaag ctgctctatt
     8281 ggatttaaaa acccaaggtg ccgaaaaaat cattttagac cttcgcggta accccggcgg
     8341 attattagga gaagccgtaa atatctgtaa tctttttgtg cccaaaggcg aaattattgt
     8401 gacgactaaa tcaaaaattg aaaaacacaa tcaaacctat aaaacgacac gtgatcctat
     8461 tgatttagat gttccattgg ttattttagt ggatggaaaa agtgcttccg ccagtgaaat
     8521 tgtttccggt gctttacaag atttggatcg tgctgttgta gttggaagtc gaagtttcgg
     8581 caaaggatta gttcaacgcc cattagattt aacctatgga acacaagtga aagtaactat
     8641 ttcgaggtat tatacacctt ccggaagatg tattcaagca ttggattatt ccaaaaaaga
     8701 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntttctgatg cagattttac
     8821 agattttaaa agctttttaa agaaagaaaa tttctcattt gataccgaga ctgaaaagca
     8881 attgaaaaaa acattagaaa cggctcaaaa ggaaaaaata gaggaaaata ttaaagtaga
     8941 ataccaaaat ttaatgaccg ctttacgcaa aagtgaggaa attcaattga ataaaaatca
     9001 gactgaaatt aaaaaactca tactagatga attgataaaa cgttaccaat acaaagaagg
     9061 actttatcaa tactatattc aaaataatag tgaggttaaa aaagcaactt caattttagc
     9121 caatcaaaca gagtataata aaatacttaa aaaatgataa aacacgggtt tgcatttttt
     9181 ctgcttttca tcagtatctc actttttgct caagaggaaa tggtacaatc catctatttt
     9241 gatacgaata aatcggatgt tgatttaaaa caaaccacac aaatggttga tttcattaaa
     9301 aagattgaca gtacacggat tgaaagtatt caaatttttg gttattgtga cgacgttgga
     9361 aaagatgatt ataacagaaa attatcgact gaaagagcca ataaagtaaa atcaaaaatt
     9421 atcgacaacg gtattaccaa taaaatcatt gtcaccatag aagggaaagg aagaatctta
     9481 attgaggatg atattgttga taaccttcca gctgttcgtt caaaaaacag acgtgttgat
     9541 gtggtgctaa atttaaagcc tttgcctaag gaagatttaa aaattccggg agtttatcca
     9601 acgtttacta aagacaatat tgtgggtgac cgcattgtgc tttctgatat gctttttgaa
     9661 agaggaagca gcgttttaac agtgaaatgc aaaaatgaat tagacaaaat ggccaaactt
     9721 ctaaataaat acaaaaatct ccacatagaa atacaagggc atgtttgttg tacgcctccc
     9781 aaccataaag aagcaattga taaagccacc aaaaaaagaa aattatctca aaaccgagct
     9841 gaaagcgttt ataaatactt gattagcaaa aaaattgcca aagaccgatt aacttttaaa
     9901 ggatatggta atacggtttc gttaggaaaa agtccggaat tggatcgaag agtggaatta
     9961 gtgataacga aactttagct ttttttcatt tcaatatgcg gaatatcatc ttcgagatac
    10021 atttcgctgg tttgaacaaa tcccaagttt tcatagaatt tttttaaata caattgagct
    10081 gaaatcgtga tataagtttc atttaaaacc gtttttgtca cctcaatggc ttcctgcatc
    10141 aaatcataac cccattttct gtcgcgataa gattgcttga caactactct gccaatagaa
    10201 gcggcttcaa aataatcgcc gggtataaaa attcggacat aagcaacaat ttcgttgtta
    10261 aaagttccga taagatgaat ggctttttca tcttttccgt caatatcctg ataaacgcaa
    10321 ttttgctcca ctacaaagac ctcacttctc aattgcagca cattgtaaag ttctgttagc
    10381 gaaagttcgt taaaactctt tattttgaat gttaattgca taaaaaaagg aagttaaaac
    10441 ttcccgtaaa tttataatag tttaatcgat ttaattatcg attctaattc gtgcattaaa
    10501 tctctttttg attgagatgg attgtaacaa aacccttcaa taattaaata acgattgttt
    10561 ttttcgtctt ttatcgcata attaataaac ggtccagcca taaaatcgtt tttcaattcc
    10621 caggttcctt tggtttcata ggttttttta ccatctaaaa ttgtgtaaaa gaaatagggt
    10681 gaatatgccg cttcagtaac cataaacgta tttggtaacg ttccctgaat atgcttttga
    10741 cctatggaat cacgcatttg aatgatattg gtcattgtat tcgaatcttt ctgtatagtg
    10801 ctcaggggaa cttcgtatat caatagactg gcatttccgg agataagctc ttttcgaatc
    10861 caaaaaaact tttcctcttc aataatataa cggtaggccg taggcaactt taaactcgct
    10921 ttaaatttgg tttttatttt cgtatcattt aataacgatt ttgcaatacg tttttggttt
    10981 tcagctattt ctgttttctt gaattttttg attatttcct gcgaatgggt ttctaataat
    11041 ttaataattt cagaaacaga acccccagaa atatgagcca cattttgagg ttgtgcaaac
    11101 tcgttttctt ttagttcaaa agcgttgttc tcctttttta aaaccaccaa aatatttctg
    11161 ctgttggtca taaaaccttc aaataaattg ggagcatatt gtttgattgt aaacaaaggt
    11221 tcttcttgag gtaaaccatc aaccggagcg gcaaattttt tacgaatgct gtcacccact
    11281 tcgccattcc acaattgatt atcaataatc acagaaaggt gattaatttt accggatgat
    11341 tctttatagg ccgttccgga cttttcctct ttgcaagaca ctataaaagt gacacaaaaa
    11401 aggagaataa aaatattttt catgatagta ttttaatagg aatgggcgat ttttatccgc
    11461 tgatttttag tttcattccc ggttttatat ttccaccact aataccgttc cattttttta
    11521 aatcagagac tgtaacacct tttatttttt tagaaatact aaacaaagag tcgccttttt
    11581 tcacaatata aatatcttct ttattaaaag tagaatgttt ttcaggtttt tgagttttaa
    11641 cctcggctac tatttcttct ttttccggaa cgataatttt taaagttgac cctaatttta
    11701 cattataatc ttctaaatta ttccatttct tgatatcatc tatacctaca ccgtattttt
    11761 tggcaattgt acttaaatta tctccgcttt ggacaacata attttcggtt ttttcttgat
    11821 ttatagccgc cgaaactaac aattttgaac caagttgaat attttcatct tttaggttat
    11881 tccattcttt taaatcctct ttagtaacgc tgtatttttg agcaatgcta tataaatttt
    11941 ctcctctttc tactaaatga aattttgtgg aatccttttc tttttctgat tttgacttaa
    12001 tggtgtcgtt tgaaatggct actgatttat ccgatgtttt tttaggagct actgcaactg
    12061 cttcatccgt tataattttc aaacttttac cggttgctac tttatttgat tttaatttat
    12121 tccatttttt taattcagaa atggtaacgc catatttttt agaaattcct cctaatgatt
    12181 ctccttttct tactttgtgg tattttgttt tagaaaccat tccttctgaa acatatgctt
    12241 ggtttgaagt aaaaggtttc tctcttttat taaaatcata ctctgtataa gcataaattt
    12301 tactttcatt agatgtgaat acagccactt tatcgattgg taatctcaca aaatgttttt
    12361 tatcagccat ataaggcaca taatcacgtt tgtattgagg atttaaaaat cgcaattgtt
    12421 ctactgaaat actcaataaa tccgataatt gtttaaaagt cattttgttt tttaccatta
    12481 tggtatctgt ttcaaaatga tttacaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnaga ttatattgct ttccggttcc gtacataaat tgccacaaac cggtagcccc
    12661 ggctttagaa acagctaacg gatttaaagc tgattcaaca acagctaaat atttaatttc
    12721 taacggaaca ttgtatttag ctaatgactc ttcaaaaatt ggaaaatagt attgagacaa
    12781 tgacatcaat cgctcgtatg ctttttttct gtttttaaga tagtgcttaa tgatattctc
    12841 taaacctttg ttgtattcaa tatgaaaagc cgatttagaa tctaaaattt tcaaccgttc
    12901 ttttaataaa tcagttgata aatcatagtc tacattattt tcaggattca cattttggat
    12961 atctgcaatc atttcgttaa acaaatcctg attgcttaat tcactcatcc aacgttcgtc
    13021 tacacaagaa gcaacttcat gcttaacaaa gctttgctta atagaatcta aataagaaat
    13081 tttaggttca actttttgat taatgctcac aattgtagta tcttgagcat gaaacaaagt
    13141 agaaaaaagt aaaacggtgg cgagtgtggt atttcgtaaa ttcattgttg ttggtaatct
    13201 tttatttatc agatgattat ctgttcttgc aaacataatt atatacaacg ataaaaactg
    13261 tttatttatt gttaaaagta ctctttaatc taaaattgcc gcaattcccg gcaaggttct
    13321 tccttcaagc atttccaaca ttgctccacc accggtagaa acataactca ttttatcttc
    13381 taatccgaat tgtttaaccg cagcaactga atcacctcca cctactaaag aaaatgctcc
    13441 attagcggtt gattcagcaa tataatttcc taaagctatc gttccgtgag caaaggcttc
    13501 catttcaaaa acacctaaag gaccattcca taaaatggtt tttgaattca taatctcggc
    13561 tttaaaattt tccaatgatt ttggtccggc atctaatcct tgccatccgt ccggaatttc
    13621 acgcacatca acaatctgtg tgttagcgtc gtttgaaaaa gcatctgcag caactacatc
    13681 aaccggaatg tggatatgta catttttttc tttagccatg tgtaaaatat ccaatgctaa
    13741 ttgtaattta tcatcttcac aaattgaatt ccctactttt ccgcctaatg ctttgataaa
    13801 agtaaaagtc attccgcctc caataatcat atggtcaact ttgtctaaaa tattttcgat
    13861 gaccgtgatt tttgatgaaa cttttgaacc accaagtact gctgttaccg gttttacact
    13921 atttttcaaa accttattta aactttcaat ttctttagcc aacaacatac caaaacattt
    13981 atgatttgga aaaaactgag caattatggt cgtagaagca tgagctctgt gagcggttcc
    14041 gaatgcatca ttcacataaa tatcacccaa agaagctaat ttttttgaaa attctaaatc
    14101 tcctgcttct tcttctttat aaaaacgaag attttctaaa agtaatactt cgcctgcttt
    14161 taaattatta gccgcttttt cagcaatttc accaatacaa tctgacgcaa attgaaccgg
    14221 aacacctaaa acttctgaaa tttttaccac aatatgacgt aaagaaaatt cgtcttgcac
    14281 cccttttgga cgacctaagt gtgacattaa aataacactt ccaccatctg ctaaaatttt
    14341 atctattgta ggtttagcag cctcaattct attggcatca gttacattaa acttttcatc
    14401 taaaggcaca ttaaaatcaa ctctaattaa tgcttttttg ttgttgaaat taaaatcaaa
    14461 aagggttttc atttttataa attttagttg ttatgtcaaa ttttcacaaa tataattttt
    14521 taaaaattaa ctatatttga tttaaatcat gttttggttt tattccgtaa tattgacaat
    14581 taaaatccac aattcctcaa aatatgtgtt ttttagttta gattcccgta aagcatctgc
    14641 ttccgctctc gtatttacaa aatccagata aacatacata aagttatttt caggactggt
    14701 aaaataatta gggtcgagtc ctctatccct aagtttcatc ataaattttg cggcatttac
    14761 acgttcatta aaaacattgg ctactagata atagccttta ggcatgtcaa tattgtttaa
    14821 tcgttgaact gaattattga ctgaattttc cagagaatta gattttttat cttccttttt
    14881 caattcggta gtagaaacta atttagaatc aaccggtttt tgtttctcgc ttgattgtgc
    14941 agatagattc tcgcttttct ctgaatttat agtgccggat gaggttacaa cttttgcttc
    15001 aggtgatttt ttagcaactt ccttttcctt aggctttgaa tttgtgtttg ccacaatatc
    15061 agttgtactt ggaactgaag ttgcgtttgg ttttgttgac ttatcttttg attttgttac
    15121 cgcttttacc tctggtttca aacttgtctc ggtttcagca acttttggct taaatgcgtt
    15181 ttcggcaatt gtttgaggac ttggttcctg aataatttct ttattggtgg ttttatcaac
    15241 caatttgaat tttttgtctt caaaaactac tttagtattg tcttgcggtt gttcaatttg
    15301 aattttagaa ggtttgattt caatgtttaa cgcacagaag aacatataaa aaacatcgcc
    15361 tgaagatttg aagctgactc tggcatcttt agcattattg cctattttag aattgtttgg
    15421 attgcgtaaa gttgtaatca acgcatcata tcccaacgtg tttttactgt ccggaattcg
    15481 atattgaaaa ttttcgtctt caattgtaat tgaactgtca aaaaagttgc tgtattttcg
    15541 tgttccggtt ttaatatttg aaagactggt atttgttgaa gaactaaaca ttaataaatc
    15601 tccatccaca ttaaaatcac cttctaaagc agaacaggca agttttacaa taacatctcc
    15661 ttccggttgg gtttcaaaat ttttaaaatt aatgtcaact ctttcactgg atgctccggc
    15721 aaaaccatcg taggaactga tatacttttc ggtcatggat aaatcttcat acacaaaaat
    15781 aattgtccac cctgcagcag aaccgccaat taatgtgcct tgagttgctt ttatgtttgc
    15841 aactgtataa aatccaaatg gattttctaa ttctttcaca tagggtgtaa tgtcagcata
    15901 aacagtataa ggagcaactt ctttgaggtt tttatctttt attccatcaa aaataatttc
    15961 tcctttaatc gatttatatt ctttttgcgt tggaaatttg accaatactt catcaaaaaa
    16021 ctctcttgtt ttatcaaaag caacaaattt atcttctcct tttttataac cactttcata
    16081 tttataggtt gcagaccaat atagaccggc ataaatgatt tttttcgatt cttcattctt
    16141 caaaaacaaa gcagcactac tagaagaaaa agtttcctta tctgtatcaa tatcgatata
    16201 ttccatatcc atttcatcat ttgaaagtgt ttcaaatgtt ttgtcattaa atggcatatt
    16261 tgtggaattt tttttatcaa tcctattcag tacagaattg gcaataatag tcatatcgcc
    16321 ttttacattg actttgtaac gttctttaaa cggaacagtc aattgtgcgt tactccaaga
    16381 ggagaaaatt aaaaaaacaa taaaaagtgc gttcttacat ctttttagat tcatatacgt
    16441 actcatattt gaatgatata aagctttggg gttcaacatt cacggagaca aaggtaataa
    16501 agacgaacga caaaaaggct tgttttgtag attaaagtaa gaaaataatg tgtttttgtg
    16561 tattagctat ttatataatt gccttttaaa gtaagatttt aaagtttgaa tctgtttaac
    16621 acagcatttt tttaggcaaa aaaatagttt atatttgctt tatgcagttt tcagaaatac
    16681 taggacaaga acatatcaaa aaccacctta ctcaaagtgt tcaactggga agaattccgc
    16741 atgctcaact ttttgtgggc ccggaaggat caggcacttt gcctatggct atagcttacg
    16801 ctcaatatct tttatgctcg aataatggag ctgaaaattc tggcggaaat gaatcttgta
    16861 atttaaaatt tcttcatttt gctcatcctg atttacattt tatttatccc acagttacta
    16921 ctgatgaggt aaaatctaaa cccaaaagca ttgattttat tcaggaatgg cgagaatttt
    16981 taaatcagaa tacgtacggt ggcctttttg attggtttaa aatactggat gttaaaaaca
    17041 aacaaggaga aattagggtc gacgatgctc aggaaatagt tagatctctt tctctaaaat
    17101 catatgaagg cggttataaa gtcatgatta tttggatggc cgacaaaatg aatattgaag
    17161 cctccaataa attgttgaaa attttagaag aacctactga cagaactgtt ttcattctaa
    17221 ttaccgaaaa tgaagaagac ttaattcaaa caattcgttc acgctgtcag gttttagact
    17281 ttaatggttt acctgaacaa gtaattgctg aagccttagt aaaatataag aaattagacc
    17341 aaaataccgc ttctaaaatt gctcgccaag cacaaggaaa ttacaataaa gctctgaaat
    17401 taattagtga agacaacgag gatttacctt ttgaacaatg gtttgtaatc tgggttcgtg
    17461 ctgcttttaa agcgaaagga aatgccgcgg ctattcatga attaattgaa tggagtgagg
    17521 aaatagcaaa aatgggtcgt gaaacgcaaa agaaattttt gacatattgt ttggaaatgt
    17581 ttcgtcaagc cttattgtta aactatcaag caacttcatt ggtttattta gaaccaaaag
    17641 ttgaaaaatt taaattggaa aactttgccc catttgtaaa cggcaataac attataaaca
    17701 tttacaaaga actaaacgac gctatttacc acattgaacg caatgggaat gcaaaaatta
    17761 ttttaactga tttatctatc aaactaaccc gtttaataca taaaaaataa acctatgaac
    17821 aatccagctt cttttttaat attacttttt ttggcgatta cttttttgca atccggttac
    17881 gataaactct tttattggaa cgataattta ggatggttga aaggccattt ttcaaaaaca
    17941 attctcaaaa atatggttcc gttagctttg gctaaaatat tgattttaga acttatttcn
    18001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna acaaaaccga atctgttttt
    18121 ggaaaaaaat aatcaaacgc ggccttaatt cgttcagaaa taaacaaaat atcaacttct
    18181 tccacttgga aaagacgatt taattgcgat ctaaatttct tggccggttc ttctaactcg
    18241 ctaattgcat tttcttgaat ttcagcccat ttttgatgtt tacttttctc agatttttcc
    18301 gcatttgcca aataactaaa acgatgattt cgccaatctt gtgctaattc agtccaatca
    18361 aaactgtttt tgatgtattc atgaatgaat cgttgcgttt ctttttgcag cgtttttaca
    18421 atggtttcat tatcggcttt attttctgca tattgcatta cttcctggtc gtttgaaatg
    18481 ccattcattt gaattggttt aagcaaaatc aacccgtcta acgagcgaag tcgcgataaa
    18541 gcaacatacg cttgtcccgg taaaaaaacc tgcgagacat ctagaacagc tttttcaaac
    18601 gtcaatccct ggcttttatg aacagttatc gcccaagcta atttaattgg gaaatgcaca
    18661 aaagaaccaa gtacttcttc gtctatttct tttgtgtttt cattaacgga ataccgaata
    18721 tttttccatt catatttttc gacttctatg attttatttt cttccggaaa atgaataata
    18781 atttcgcctt cagacaatga tttgacaaag cccattttgc cattgaaata atttttttca
    18841 ggagacaaat cgtttttaat aaacataatt tgagccccaa ctttcaattg caaaatttct
    18901 tcaaccggat aaattttttc aggaaaatct cccacaattt cgggtttaaa tcggaaacat
    18961 ttctctttaa tttcgtctaa agaagctgca tttatttcgt ctgcttttgc attgtgtgtg
    19021 gttaatgtaa tataaccttt attggatttt atgtcgaatt ttggatttac aaaatcattt
    19081 aaaatcatca tatcctgtga tgaaatcata ttgtttcgca agttatttaa taccgatata
    19141 aaccgctcgt cagattgacg gaaaatctta gttaattcga tataaagcgg tggactttct
    19201 tgaattactt ttgcattaaa aaagaaaatt cctctataat aatttcttaa aacccgccat
    19261 tcttcatttt ttacaacggg cggtaattgc agtaaatcac ctataaatag aacttgaaca
    19321 cccccaaaag gaaaatcttt tcttcttatt tttcgtagtg taaaatcaat tgcatctaac
    19381 aaatcagccc tcaacatgct gacttcatca ataataagca attccatgtt aagcataaca
    19441 cttttctttt gtccgctcat tcgaaaatgc ttgaccaacg tgtttttggt ttcaaatttt
    19501 tgataatcag agatgcttga accgggaata tattcgggaa taaaaccact aaacggtagt
    19561 tgaaacatgg aatgaatcgt aactcctcct gcgtttaacg cggcaattcc tgtgggagcg
    19621 actaccaccg tgtttttgtg agtagtttga ataatctgtc gtaacagcgt agtctttcct
    19681 gttccggctt ttccggtcag aaaaacagac cgttgtgttt gattaataaa ttgaagtact
    19741 tggcgtgcat cgtttgtaat ttccatttgg cgttgagttt agaaatgtga aaatacaaat
    19801 ttttgattaa atagaaatta tttttgacat caataaacaa aaaacctctc gacgaggaga
    19861 ggttaaagta ttgaaataat atttcaatta tttttttgct tctgcagtat cttctttctt
    19921 agtttcagtt gaagattttg ctttgtattt atcgtttagc aatttaataa tctcttttgt
    19981 gatatcatat tcctcttttg catacaaaac agaagcagca tcacccgtgc catatatata
    20041 gttatatcct ttttctttac cgtaatcttt gatgaatttt tttacatctt ttactaagct
    20101 atccatctct tttccacttt cttcttgtaa ttgacgtaag atagcttgtt gagcataaga
    20161 caactcttgt tcttttttct gtaattcagc tcctttttgt tgagcccatt gtggtccgta
    20221 agtttgagca tttttttgaa aactagaagc ttcacttcta aaacgagcta cttctgcttc
    20281 taattgcttg cccatttctt ccccttttga tttatatttt gcttcgatat ctttcgcttc
    20341 ggtatattct tcaagaagat ttgaagtatc aacataagcc gttttgaaat ctttttgttc
    20401 ggctgctttt tcacaagatg ctagggctag aattaagcca aaaactaaaa ttgatttttt
    20461 cattctgttt gtctaatatt taaattttgg taaaagtagt aattccaagt gaactaacaa
    20521 atttgattta ttttaataaa attcaatact ataattaata ttgatgcttt taaggttttt
    20581 tattagtatt agtgataaga aacagccttc atattttgaa ttaaatcaac aattttaaaa
    20641 caattatgga aatgttggtt aattgtaaaa agatcagtta attgtgttta aaataagttt
    20701 taactgtttt taaagatgta aatatgggat gaatattctt ttgtttgcct tcctttgaga
    20761 tttgattgta atccaaccca aaaagcggag aacagattca tttttccggt tttatatttt
    20821 tctgaaagta aactgacata aaaactatca aaccacatnn nnnnnnnnnn nnnnnnnnnn
    20881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20941 nnnnnnnnnn nnnnnnnnac atcaaaaaaa tggtcttcta actctgaata attatctaca
    21001 atgcatattc cctttttctt tgagatggtt tttgctttac tattaggttc catccctaca
    21061 gctttccatc ctctactttt tgcaactaat aaaaaatcac cggttcctgc accaatatct
    21121 aaaaccgttc cttttcctgt gtgccaatat tcaattagtt ttactttatc atttaaagct
    21181 ttctttttaa taaattggta aactttttca aacaaagttc gttgtccatc agtatgggaa
    21241 atataatcct cgctttcata atagttaggg agcaattcta aagagggttg tggatacgtt
    21301 ttcagtaact ggagatcttc atctaataac aattcaaatt cttctcccga tacagagtga
    21361 tcttttactt taatgtaggc ttgttttaaa aaattcattg taataaataa agtgttatta
    21421 tacttgcaac acttgtgttt gttaggtttc ttgaaatagt ttcacgtgga acagttatat
    21481 taaaattcaa attaagcaat taagtgagca ctatttacgc cttaccttcc catataaatt
    21541 aataaaatag aaatgtcact aggggaaaca ccacttattc tggaagcttg agaaactgtt
    21601 acaggacgaa tttttttgaa cttttccctt gcttcgtatg agagtgattt tattttatca
    21661 aagtcataat tttcaggtat tttcacatct tctaaccgtt ttaatttatc tgcattgttt
    21721 cgttcttttt caatataacc attatattta acttggattt ctgcttgttc taccgtttcg
    21781 tcatccaatt ggtttgttga aatatattcc tttacttttt gaaactttac aatatcttct
    21841 aattcaattt gaggtcggga aaaaacttta tacattttat cagattgcac cattaaggca
    21901 cttcctttct cttctaaaac cggattagct tctgcttgtg ttatacttgt ttcgcggaaa
    21961 aaattaacca tcttctcagc ttccttaaac ttatactcca tacgtattaa acgctcttga
    22021 gaggctaaac ctagagcaaa accttttggc gttagtcttt catccgcatt atcttgccgt
    22081 aataaagtgc ggtattctgc tctagacgta aacattctgt aaggttcttc agttcctttg
    22141 gtaattaaat catcaattaa aacaccaata taagcttcat ctctttttaa aataaaaggc
    22201 tcacgttctt gcacttttaa ggcagcgtta atacccgcca ttagaccttg agaggcagcc
    22261 tcttcatatc cggttgttcc attgatttgt ccggcaaaaa acagaccttc aatcaacttg
    22321 gtttctaatg tgtgttttaa ttgagtaggt ggaaaatagt catattcaat agcataaccg
    22381 ggtctgaaaa attttacttt ttcaaagccc tcgacagaac gcaaagcttt gaattgaaca
    22441 tcttccggca atgatgtaga aaacccattg acatatactt caaccgtatt ccaaccttct
    22501 ggctcaataa ataattgatg tctgtcttta tcagcaaaac gattaatttt atcttcaata
    22561 gacggacaat atcttggacc agtactttga attctgccat taaacattgg tgaacggtca
    22621 aatccttctc taagtatggc atgaacatcc aatgaggtgt atgtcatcca acacgatttt
    22681 tgttccacta atggtttaga agaatctaaa taagaaaatt tatgtggctt ttcatctccc
    22741 ctctcttctc tcattttaga ataatccaat gaacggccat caactctagg cggagtaccg
    22801 gttttcattc ttcccgattc aaaaccaacc ttgattaaat cttctgtaat tccataagcg
    22861 gcactttctc cggcacgacc tccgccaaat tgtttttcgc ctatatgaat taatccattt
    22921 aaaaaagttc cgtttgtaag cacaactgtt ttggctttaa tttcaattcc gagagaagtt
    22981 cggattcctt taataacgtt attctcaata ataagtcccg ctaccatttc ttgataaaaa
    23041 tcaacatttg gtgtgttttc caacatcaaa cgccattctt ctgcaaaacg cattcggtca
    23101 ctttgcacac gtggtgacca catggcagga ccttttgatt tattcaacat tttaaactga
    23161 atggctgtct tatcggaaac aatgcctgaa tatccaccta atgcgtctat ttcacgtact
    23221 atctgccctt ttgcaattcc acccatagca ggattacaag acatttgacc gatagtttga
    23281 agattcatcg taaccaataa agttgtacag cccatattag cggcggcggc ggcggcttca
    23341 gaacccgcat gacctgcacc cacaacaatc acatcatatt tttcttgaaa catagttttt
    23401 attaaaataa ttgttccacg tgaaacatcc cgtagaacaa aatatattta tgactgttcc
    23461 acgtggaaca tcttcatttt ttcttcttct ttttgacgca ttattttttc ttcttcggca
    23521 cttttgtctt tatatccaca atagtgcaaa ataccatgag ccataactct ttttagctct
    23581 tctaacaaag aaatattaaa atccgttgca ttatcttgta ctctttcaat agaaatgaaa
    23641 atatcgcctt gtaaaaaatt tccttcgcta taatcaaagc taatgatgtc tgttaacgta
    23701 tcatgattta ggtactgaag attgattttg tgtagataat catcatcaca aaaaatgtaa
    23761 ttaatctctc cttctaatct attttcagat tcaataattt tggcaatcca atttgaaaac
    23821 aacgtttcgt tacctaattc aaaatctgtt tcgtaattaa agctaatcat tttttttaaa
    23881 atattcttga accttttggt tgtaattggg ccgcaaaggt aagctttgtc tatttaatat
    23941 ttcaacacta tttaaatatt gttgtaattc aggagaaaga ggtttaacgg cattattata
    24001 ttctttttta ttcgtttcag attgacgttt agtgtcttca ccctgctgtt gtagtgcttt
    24061 ttctaactta agcatttcat aattgatatt taacatacgt tgcaaaactt catttttgaa
    24121 tcctttgttc aacatttgtt tttctgcatc cttcatttga tctaaaactt tttgaccact
    24181 aggtgtcaat ccttgtttct ctaaagcatc ttgaagttgt tcccgtagac gtctttgctc
    24241 ttgataaatc tccattaatt cacgagcatt ttcttctccg tcactattgt tttgaccacc
    24301 actgccagaa ccttcaccag gtttattccc tttttggttt cctttgcctt tttcaccatc
    24361 tttgcctcca ggtttatccc ctttatcacc ttttttgcca tctcccggtt tttcgcctgg
    24421 tgattcacct ggtttgtctc ccggtttatc tcctttttcc attccctcct tcattttatc
    24481 agacaatcct ttttgttttt gaataatgtc cggcaattgc attcctccct gacctttacc
    24541 tggttttggt ttaccggcac cagcaccgga catcgacatt tgcatactgt ttaaaacatc
    24601 tgaaaggaaa tcagctaagc gattagaagc cgcaaccgta tattgttgat gtgaaactcc
    24661 ttttgaaata atagcttccg ctaatgactc taaagattta tcgacgttgt attgaacatt
    24721 accaatttct tctgtaactt tttctgtaat catagcattt cgcaaagaca tcgcaaataa
    24781 actatcgtca acatgcttaa actgttgttt tagatcttgt tgttgtttta agtatttgtt
    24841 ataggatgga gcaccttttt ttaaactttt aaattgtttc attaaatctt cttgtgaaaa
    24901 agaaaaagct aataaattgt ccaaaatttg acgcatcatt tttacatctt cttctaattg
    24961 ttccatttca cctccagcca tagattccat catttgctgg ctcatttgtt tcatcttttg
    25021 agaagcactt ttttgctttg gcttggcttt ctctttttgt tgtttctgaa gttcatctga
    25081 agccttttgc aaatcttctt ttatgctttt ttcttgtttt tcgtcagaag gaatatcaac
    25141 agggcttttg agttctttgt tgtccttttc taattcgcgt aattcttctt ggattttctc
    25201 aaattcttta ttgatatcat tttgtttctc tgttgtattt tctttctctt gcttagctaa
    25261 gttatcttgt ttttctgcta atttatcaag cttacttgct aattgttccg cctttttttc
    25321 tacataatat ttcttagtca actcaaccaa ctgttctaag tttttggttt gattttttgt
    25381 gctttgtttg attttgtcca ttttatcaaa catttccaca tcgtttagtt tttcggccaa
    25441 tttttcgagt tcgtctaata atttttggtt tttatcagct tctttttcag cattttctgc
    25501 tcgtttttcg agttctttct taaactcatc tttattgtct gatttagcgt ttttgaggtc
    25561 ttcttggagt tttttggtaa actctttcat catttcttcc tgttgctttt gacgtttgat
    25621 gaaatcgtta attttttgtt tgtctttaaa ctccaggttg tttttttctt tgcccatttt
    25681 ttggatttta tccaactcgg acaattgttt atcttgattt ttaagcgatt tttgaaggct
    25741 attaatatta tcattttgct gtttcaattg ctcatcttgt cgttcttctt ctgtgttaat
    25801 tctgtttgaa aaagtagatg attttgaact cttaaaatta tgcaaagcat cattatcaaa
    25861 aacttcaaaa tagtattcat aagaaacacc ttcttccaca ggaagattcc ctgggaaagt
    25921 aaaaatgaat tgatcaaaaa catccttctt aacgggaaga acagatttta aggctgcttt
    25981 atcattgttt tgaggataat agac
//
LOCUS       DAOTCW010000016        25067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1001_length_25067_cov_42.156125, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000016 DAOTCW010000000
VERSION     DAOTCW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 25067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25067
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1001_length_25067_cov_42.156125"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1836
                     /locus_tag="P5335_02420"
     CDS             220..1836
                     /locus_tag="P5335_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HRZ73767.1"
                     /translation="MFLFLLFISFPFLQANAQKNKEIFVEYSEFFDVDETLYPDAILL
                     TGNVRVSHEGAILYCNKAYLFKKENYLKAFGDVRMVQGDTITMTSTYAEYNGENKQAY
                     ASGNVVMTSPSSTLKSEIVNFDRNTQQAFYKTGGTINDKENVLTSQAGTYVVQKKMYQ
                     FRNAVTITNPKSVIKTNHLDYYENVGHAYLFGPSTITSKENYIYTEKGFYDTKKNLAH
                     FVKNSYIKYNNRLIEGDSLYYDRNREFASATRNVKVTDSINNSIVKGHYGEVYKAKDS
                     LMMTKRAVAMTLVEQDTLYIHGKRLIVTGKTEERVIRGYNNVRFYKTDMSGKCDSLHS
                     SEKTGLTKLIGNPVMWNYDNQMTGDIMHLISNKETEKLDSLKVLNNTFIASKDTIGSG
                     YNQVKGQNLYGKFRDNKLYEVDVVKNTEVIYYMRNDEQELIGINKSASSSINMTLDGN
                     EIEAITFFTKVEGEIYPEKDLPENARKLKGFIWRGDERIQSKEDIFPPEENEYDEKMK
                     QETKAEEEKEAVPMEVRKESLEVEEKKKDS"
     gene            1908..3104
                     /locus_tag="P5335_02425"
     CDS             1908..3104
                     /locus_tag="P5335_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRZ73768.1"
                     /translation="MKEDFLKYQAQTSPYPLAMEVSYAKGSYIFDTAGKKYLDFVAGV
                     SASSLGHQHPRVNQAIKEQLDKYSHVMVYGEYAQHPAVEFCKLLAKHLPEPLDKTYLV
                     NSGTEATEGALKLARRVTGRSHLVSCFNAYHGNTMGSMSVMGFEERKQVFRPLVPDVD
                     FITFNNEADIVKITTKTAGVILESIQGGAGFIEPENGFLRKIKARCDEVGALLILDEI
                     QPGFGRTGKLFGFENYEVVPDIVIMGKGMGGGMPVGAFTAKAEYMDLLSDHPKLGHIT
                     TFGGHPVIAAACLATLREVTETDLIPQTLVKERLFRELLVHPLIQEVRGKGLMLAAMV
                     ETPEITNQVIFKCQDKGLILFWLLFEGRAIRITPPLNISEEEIREGCAIMLEALDEVM
                     KETADQ"
     gene            3223..4620
                     /locus_tag="P5335_02430"
     CDS             3223..4620
                     /locus_tag="P5335_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRZ73769.1"
                     /translation="MHLSHEEDDYNISLSKFESMLKTNKVLFFDSEEFEEIILHYLDM
                     GKASLAKKALKLALEQHPKSTGLKLVQIEMLIYDDKLDVAEKMLNELYAIEPHNEEIY
                     IQKANIYSKRDQHEKAIEELEKALTFTDDFADVYNLMGMEYLFMDNLELAKYNFIKCL
                     EVDIEDQSALYNVVYCFEFLDQQKEAIDYLEKYIEKNPFSEIAWHQQGRLSYSIKNYE
                     NALRCFDYATLIDDEFIGAFMEKGKSLEKLKRYEEAIEAFQRTIELEDPTSYALLRIG
                     KCYEKLNNKAEAIKYYDKTVHEDPLLDKGWIAITDFYIRLKNYQKALYYVNKAIAIDN
                     ENQLYWKRYATINRQMNFYEEAEFGYRKAVEFGDYQLDTWLFWVDILQFLGEFDSAIQ
                     TLLQAAEYFPEQYEVEYRLAGIYYMLQQNEKGKTHLNNGLRINAKNRTVLQELFPVVW
                     DKKVVQNVITKHLKS"
     gene            4693..5658
                     /locus_tag="P5335_02435"
     CDS             4693..5658
                     /locus_tag="P5335_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HRZ73770.1"
                     /translation="MRKFFPDYFLISLKGLAMGAADVVPGVSGGTIAFISGIYQELID
                     TINKVDFSFFASWKKHGFKIAWQQINGSFLLALLSGIAISILTFSKIITHLLATEPIM
                     VWSFFFGLVIASIFFVGKQIDKFSIGSISFFLLGTALSYYITIAEPVASPDSYPYLFL
                     SGFIAIIAMILPGISGAFILLLMGSYAVVIGTINHFREGILSLNFQVIFQAVLKLGTF
                     AIGAILGLKLFSKVLHWMFDNHRNSTLALLTGFMLGSLNKVWPWKEVISTRIDSHGVE
                     VPFMEKSILPTAFNGDNQLLVAILLMIAGFLTIFIVEKVGAKFSK"
     gene            5698..6354
                     /locus_tag="P5335_02440"
     CDS             5698..6354
                     /locus_tag="P5335_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRZ73771.1"
                     /translation="MDFFDLLIKFLSQPDQILSKLIEKYDNWIYAILFFLVFAETGLI
                     VMSFLMPFLPGDALIFAIGMIAARGELDIVIIVPLLIFAALLGDNLNYYVGKRFGSFI
                     LNSEKTFIIRKSHILRAQEFFDKHGRNSIIMARFIPVVRTIVPFLSGATNVPYKTFLK
                     FSFIGAFLWVAAISLLGYFLGQIDYVKEHLEKFIILIIIVANIPLIKQFLPRKKAKNE
                     "
     gene            6347..7123
                     /locus_tag="P5335_02445"
     CDS             6347..7123
                     /locus_tag="P5335_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRZ73772.1"
                     /translation="MNKLFGLIGKNISYSFSKKYFTEKFEKEQLTDCAYENFDLTAIS
                     QFPKVIRENATLCGLNITIPYKEKIIPYLDKLSENAQKIGAVNCIKFTKKGKLKGYNT
                     DYYGFKKSLQPLLQPHHQKALILGTGGASKAVAFALEELGILYTFVSRSKKENALDYK
                     YINATTFDNYQLIINCTPLGTHPNIEECPPIPYDYFTPEHLAYDLIYNPEETLFLKQA
                     KSKGAVTKNGYEMLVLQAERGWKIWNKKSISSFSNTDTAI"
     gene            7546..9366
                     /locus_tag="P5335_02450"
     CDS             7546..9366
                     /locus_tag="P5335_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HRZ73773.1"
                     /translation="MSENGHQIALNAIDESNAEESEDETLKERHNIPMLEYASLSMEA
                     LVDELEKLTEVEKVMSVKEHVEEIKKEFLAKYHHFIDEKRDEFKAENPDSTEDFEYHL
                     PLKSKFDTLYKQLKGKQNAHFKSLQNNLNANLDRRLAIVEEIKNLINPQENIKDTLKI
                     FNELREEWRTIGPIPRDKYNHVWNNYHFHVENFYDYLHLDREARDLDFKHNLEQKQKI
                     IARVEELVNEVDITKAFRELQDLHRIWKEEIGPVDREHREEIWNQFSALTKQMHDKRE
                     GLFANQREKETANLEKKKEIIAEIEKLATEEVTAHSQWQGLIEKVEALRNDFFSAGKV
                     PSEINEATWAEFKTVVRNFNSHKNSFYKDIKKEQNDNLSKKMALVEKAKTLSLSEDFA
                     ATTPLMKQIQEEWKQIGHVPRKYSDKVWKEFKDACNQFFDRMKATRNEANSEEIEAFD
                     KKKAYLDALKDFQLTGEHKADLEAIKAHIEQWKSFGKVPFSRRHVEGKFNKILDALFE
                     KLSLSKKDGDMMRFSNKLEQLAGSDDHRKLESEKIFIIRKIEELQSEIFQLENNIQFF
                     THAKKDNPLVKEVFKNIEKHKDELKTWKDKLKQLRSLKQE"
     gene            9465..9812
                     /locus_tag="P5335_02455"
     CDS             9465..9812
                     /locus_tag="P5335_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73774.1"
                     /translation="MKWIKNSALLAGLFILFLFGLQSHATPEEKSSNKEQQIQSIEFI
                     KSVGTLQSVGENQIQVIVKENTTTTYSKHFQFFFEKQVTFLPTKFRTFLSRQDHNRCE
                     KVSKNLFPFHFFW"
     gene            9900..10388
                     /locus_tag="P5335_02460"
     CDS             9900..10388
                     /locus_tag="P5335_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73775.1"
                     /translation="METSTTLIGLFLILLTALPIFLLLRTQHKNKSKIETILKQFNQG
                     NSKEFNLRESLNNKIIAFNEMKKKLVLIDLNIKPEIVTYVDLNEISNSKIIREMEHFD
                     GSKSTKEFITKVEILLHNKKNQQDETLKFYDYEYEKPIQLNYFRDNQLAEKWLEIINK
                     VI"
     gene            10555..11913
                     /gene="hemN"
                     /locus_tag="P5335_02465"
     CDS             10555..11913
                     /gene="hemN"
                     /locus_tag="P5335_02465"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HRZ73776.1"
                     /translation="MSLIQKYNVPGPRYTSYPTVPYWDESHFSLSKWKETLQQSFLES
                     NAAEGISLYIHLPFCESLCTFCGCNKRITKRHDVEHPYIEALLKEWELYCELLPKKPI
                     IKEIHLGGGTPTFFSPENLERLINGLFENATKHEEHEFSFEGHPNNTTRAHLQKLYDL
                     GFRRVSFGVQDYSETVQKAIHRLQPFHNVAKVTFWAREIGYTSIGHDLIFGLPFQKIE
                     DVLDTIDKTNSLQPDRLAFYSYAHVPWIKGNGQRGFKDEDLPKDDEKRLLYEVGKKHL
                     SEKGYHEIGMDHFALATDSLYHSFKNGNLHRNFMGYTSSKTQVMIGLGVSSISDSWYS
                     FAQNVKTLEEYYDLLAQNELPIFRGHLLTTEDLIIRKHILNLMCQFHTTWAEYGTYFS
                     ELPEVLGRLEEMKNDGLVQITENAITVTEKGKAFVRNICMAFDLRMTRNVPDTELFSM
                     TV"
     gene            complement(12007..14136)
                     /locus_tag="P5335_02470"
     CDS             complement(12007..14136)
                     /locus_tag="P5335_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRZ73777.1"
                     /translation="MANFDSNHVKNVVLLGHTGSGKTTLAESMLFEAGIINRRGSIED
                     QNTVGDYSDMEHEKGKTLFSKLMNSKWRGFKINILDTPGYDDLSGEIISAMRVADCGL
                     MLLNSRMGVEVGTDIIWQYTNDYKLPMLLAVNHLDNDKSDFDKTIAEAKEHFGNQVVV
                     VQYPVNQGLNFDSIIDVLNMVMYKYSKAGGKPEKLAIPDAEKEKANQLHQELIETIAG
                     NDENLMEQYFEKGELSEDEMKSGLHHSLIKHELFPVFCVSAKLNMGAGRLMGFIDNVC
                     PSAFEMPKQKTIAGEEINCSEKGNKAIFVYKTVSEPHVGDLSFFKVLSGKIKVGDDLT
                     NENTGQLERITQLFEMEGNKRIPVDELVAGDIGATQKLKNTHTNNTLHEKGFPIEITP
                     IQFPNPTYTIAVEGTKKGEEEKLSSALHQLVEEDPSIRVEVSAELRQTLIHCQGELHL
                     AVIKWKLENAHKLSVRFQKPKVAYRETIQGGADTVYRHKKQSGGAGQFGEISMKIEPW
                     YDGMPEPTGVSIRGKEEIELTWGGKLVYYNCIVGGAIDARFHPSILKGVMEKMQEGPL
                     TGSHVQDVRVIVYDGKMHPVDSNDLSFKIAGMMAFKENFVNANPQLLEPIYKVTVTAP
                     EEITGSIMSTIQSHRSMVEGMDSEGHFTIIKAQIPIAEMHEFTGDLRSLSQGRARIAM
                     EFDHYESVPYEIQNKLVADFAKEAVEV"
     gene            complement(14382..14807)
                     /locus_tag="P5335_02475"
     CDS             complement(14382..14807)
                     /locus_tag="P5335_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73778.1"
                     /translation="MKKNILILLLVTTLIGCSKNETTEETSFQEELIGSWQFKGIYIH
                     DVFDENDMPLYMAYENGDIITYNSDNTFIHLLQNYQYTGTFSVVNSNILNLNYNPNPN
                     GINNNGSSKITLLTENELRLSCFNEGLCDVIRYEKVVAD"
     assembly_gap    15024..15123
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15150..15854)
                     /locus_tag="P5335_02480"
     CDS             complement(15150..15854)
                     /locus_tag="P5335_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRZ73779.1"
                     /translation="MLYTALLFGLISSFHCIGMCGPIAMMLPIDRSNQTKKVFQILLY
                     HLGRLTAYGMLGLFFGILGKGLFLAGFQQKLSIFVGIMMIIFVIVPEKKLVQYNFSKP
                     IYKLISKVKSHLGAQFKRKSPDALFTIGLLNGFLPCGLVYAALFGAIAMQSELLGVAY
                     MVLYGIGTIPMMSAVVLISNFITVPVRNRIQKLIPIVVILIGSLFIVRGMGLDIPYIS
                     PNTTSLVVQANPDCHN"
     gene            complement(15902..16348)
                     /locus_tag="P5335_02485"
     CDS             complement(15902..16348)
                     /locus_tag="P5335_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRZ73780.1"
                     /translation="MKINWGTGIVIAFGLFITFILYFVFKVQSDSQYDNELVVEEYYK
                     QELKFENQMTKEQNAIDMTEKISISTSEEGINITFPSDLDLTKVTGKVSLYRPSNQKL
                     DFEVPISLSGPHLLIPKSNLVGGRWDISLDWTYDGKEFLNKKTIYF"
     gene            complement(16412..17830)
                     /gene="ccoG"
                     /locus_tag="P5335_02490"
     CDS             complement(16412..17830)
                     /gene="ccoG"
                     /locus_tag="P5335_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRZ73781.1"
                     /translation="MSNIKDDSFRDSISTIDKEGKRSYIFPKKPKGPFYDKRKLLSYF
                     LLAFLLSAPFIKINGHQFLMFNVLERRFNILSFPFWPQDFHLFVISMIIGVVGLALFT
                     VAFGRIFCGWFCPQTIFMEMVFRRIEYWIDGDRGAQIRLQKQEWNAEKIRKRVTKWIV
                     FFIISFIIANVFLAYLVSSDRLLLMIKEGPALHTSTLIALLIFTAVFYFVFAWFREQV
                     CIIACPYGRMQGVLLDNKSINVSYDHVRGEKELGRAKFNKQEDRASTGKGDCIDCKLC
                     VHVCPTGIDIRNGTQLECINCTACIDECDTIMESVGLPKGLIRYASEDEIVKKEKFQF
                     TTRMKGYAAVLTILIGVLIGMLFLRNDVEATILRLPGQLFEHKGENISNVYTYKIVNK
                     TTKEFNHVTFKLIEPKGTIKLVGNAEVKVKADGIAKGTLFIEINPAYLEGDKIKLKIE
                     VYDGENLIETATTNFMGPRSFN"
     gene            complement(17889..18845)
                     /locus_tag="P5335_02495"
     CDS             complement(17889..18845)
                     /locus_tag="P5335_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HRZ73782.1"
                     /translation="MKKLIPSYVRVPLIFAIVMGAMEYFIDSGNQPAFIKFPMVALFL
                     GVFLFLLVAIEIVVSAVDKVTYHLLTDEQKKQLEEAQSLPFTEGKFYQGIVQKLTRSK
                     EMEQEHDVLLDHDYDGIKELDNVLPPWWVGLFYITIIFAIVYTIRFHIMGDYTQAEEF
                     ETEMAVARAEVEKYKLTAPDLLDKDKVTLLTDAGALAEGKKIYETNCVACHRPDGGGA
                     IGPNLTDEHWILGGGIKNVFNTLMEGGREGKGMVSWKAIIKPSDLQKVASYVLSLQGT
                     NPIDAKAAEGDVWVDENAPKAEGAIETPIDSTKTTDKVVAQN"
     gene            complement(18869..19036)
                     /locus_tag="P5335_02500"
     CDS             complement(18869..19036)
                     /locus_tag="P5335_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HRZ73783.1"
                     /translation="MLKFVKHTMDSDGIEIYPIISLSIFFVVFVAYFIYAMTYKKETI
                     KELSELPFKDN"
     gene            complement(19042..21234)
                     /gene="ccoN"
                     /locus_tag="P5335_02505"
     CDS             complement(19042..21234)
                     /gene="ccoN"
                     /locus_tag="P5335_02505"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HRZ73784.1"
                     /translation="MEKQQFYYDNKIVRNFLYATIAFGVIGMLVGLLVAFLYLFPNLT
                     DGIPWLSYGRLRPLHTNAVIFAFVGNGVFAGVYYSLQRLLKARMYSDFLSKFNFWGWQ
                     LIILGAAITLPLGFTSSKEYAELEWPFDIAIAIVWVAFGINMIGTIIKRRERHIYVAI
                     WFYLATFIAIALLHIFNNLEIPVSALKSYSVYAGVQDALVQWWYGHNAVAFFLTTPIL
                     GLMYYFVPKAANRPVYSYRLSIIHFWSLIFIYIWAGPHHLLYSSLPDWAQNLGVVFSV
                     MLIAPSWGGMINGLLTLRGVWDKVRTEPVLKFYVVAITGYGMSTFEGPMLSLKNVNAI
                     AHFTDWIVAHVHVGTLAWNGFIMFGMIYWLFPRLTKTNLYSTKLANFHFWIGTLGIIL
                     YTLPMYVAGFTQASMWKQFNPDGTLVYGNFLETVTQIMPMYWMRAIGGTLYFVGIIVL
                     VYNVARTVMAGSPVENELAEAPALTKISPHQVKGERWHAWLERKPIQFTLLTTVAVAI
                     GGIVQIVPMLVVKSNIPTIAAVKPYTPLELQGRDLYIREGCNNCHSQMIRPFRSEVER
                     YGEYSKSGEYVYDHPFLWGSKRTGPDLKRVGGKYNDNWHFNHMWDPQSTSPGSIMPGY
                     KWMYDNKAMDVSYIEKKMEVMVTLGVPYTDDEIKNARKHMDEQASAIEKNLFSDPDFV
                     KSYEQSKKAAATRGEEFVPMKNREIVSLIAYLQRLGKDLKVKETAQNK"
     gene            complement(21237..21443)
                     /gene="ccoS"
                     /locus_tag="P5335_02510"
     CDS             complement(21237..21443)
                     /gene="ccoS"
                     /locus_tag="P5335_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HRZ73785.1"
                     /translation="MSVIYLLISVSIVVALIFLIAFIKAVKSGQYDDDYTPSVRMLFD
                     DEVKKNPNKNPNKNQIQTLEEKQV"
     gene            complement(21525..23900)
                     /locus_tag="P5335_02515"
     CDS             complement(21525..23900)
                     /locus_tag="P5335_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HRZ73786.1"
                     /translation="MDIGNCFHCGNEIIKKEEIIFDEKSFCCNGCKTVYEILSQNDLT
                     CYYDFQAAPGATPLDIQGKYDFLDNEEIVAKLLEFQEDTTHIVSLYIPHIHCSSCIWI
                     LENLQKLQSGISTSQVNFPEKKVRVTFNPEKTTLKKIVELLCSIGYEPYISLENYEQG
                     KKLVDRSLIYKIGIAFFCFGNIMLLSFPEYFEVEEFWINQYRGFFRWLIFALSLPSFL
                     YSASGYYVSAWKSIKSGLLNIDIPIALGIVVMFVRSTVDIVFGYGQGFFDSMTGLIFF
                     MLLGKLFQKKTYDFLSFERDYKSYFPIAITKILSDGTETSVQVYDIEKGERLLIRNQE
                     LIPVDGILISEKASIDYSFVTGEAIAIEKKSGDKIFAGGKQMGKVIEMEVLFSVSQSY
                     LTQLWSNDVFQKKVEIKHKTVTDKISRYFTPALLLLAIVSFLYWVFIDVTTAFNVFTA
                     ILIVACPCALALTAPFTLGNVLRIMGKRKLYLKNATVIEQLAKVDTIVFDKTGTITTN
                     KKTSITYEGAQFTSDELKLLKNTLRGSNHPLSRRLYDFIPNQERMEQNEFQEIIGKGI
                     FAKFGDTTIKLGSSQFLAHMSENTHKKTKVHVEINGAYKGSYIFNNQYREGLEFLFEN
                     LAKKYDLVVLSGDNDGERKILEKMLPKNSTIVFNQKPEQKLNFIKNLQKEGCNVMMVG
                     DGLNDAGALAQSNVGISISENVNVFSPACDGILDASQFEKIGFFMQYSKNAMKTIYMS
                     FGVSLIYNFIGLSFAVTGNLSPIVAAIIMPLSTITIVSFVTIVTNVYSRIK"
     gene            24005..24685
                     /locus_tag="P5335_02520"
     CDS             24005..24685
                     /locus_tag="P5335_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRZ73787.1"
                     /translation="MSKCEHCIVREFSSLKALNKDELLKIASCKTSYTIKKGEPIFEE
                     GESVNGIYCIKDGVCKLSKLSANGKDQIVKLVKSGELLGQRSMISDEPANLSAVALED
                     MEVCFIPRSEVLQFFNNNNQFSMNIMKSICGDLKESDDHMVSMAQKTVKERLAETLLY
                     LEDTFGKNEDGSLHIQLSREELAGMIGTATESCIRLLSEFNKGGVIDLNGKRISITDR
                     NKLKRLSE"
     gene            complement(24742..>25067)
                     /locus_tag="P5335_02525"
     CDS             complement(24742..>25067)
                     /locus_tag="P5335_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ73788.1"
                     /translation="IPTVAGTYNVTFNRLTGEYNFATLSVGDFTTSKAKVYPNPSQNV
                     WNFSIENDSIEKIQIVDVTGKIIVDKLISSNLATIDATALTSGIYFAKVKTNTAVQTI
                     KLVRN"
BASE COUNT         8581 a         4171 c         4175 g         8040 t
ORIGIN      
        1 aagagttgtc gaacgaggtt aaaccaatta gggttgaatg atagtgattt tggattttag
       61 gtaagatttc aagctaaaaa aggctcgtta agcggtttat tttgtttgaa atacaagcaa
      121 cttgaagacg gtggtttgag ggtatgccta aattattcct aagacgtatt gtccttccct
      181 caattttagt aattttgaca aaaaaattta cgcttgagaa tctttttatt tcttcttttt
      241 atctctttcc cgtttttgca ggcgaacgcc caaaagaaca aggaaatttt tgtggagtat
      301 tccgaatttt ttgatgtgga cgaaacctta tatccggatg ctattttgtt gacgggcaat
      361 gtgagggtga gtcatgaggg agctattttg tattgcaaca aagcgtattt gtttaaaaaa
      421 gaaaattacc tgaaagcgtt tggcgatgtg cgaatggttc aaggcgatac aatcacgatg
      481 accagcacgt atgctgaata taacggagaa aacaaacagg cgtatgcgtc gggtaatgtg
      541 gttatgactt cgcccagttc gacgttgaag tcggaaattg tgaattttga caggaatacg
      601 cagcaggctt tttataaaac cggcggcacg attaatgaca aagaaaacgt tttgacgagc
      661 caagcgggca cgtatgtggt tcagaaaaaa atgtatcagt tcagaaatgc ggttacgata
      721 accaatccaa agtcggttat taagaccaat catttggatt attatgaaaa tgtgggtcat
      781 gcctatcttt ttgggccctc gaccattacc agtaaagaga attatattta tactgagaaa
      841 ggtttttatg atactaaaaa gaatttagcc cattttgtta aaaactctta tatcaaatat
      901 aataatcgat taattgaagg cgatagtttg tattatgacc ggaaccgcga atttgcttcg
      961 gcgacgcgga atgtgaaagt aacggattct atcaataaca gtattgtgaa aggccattat
     1021 ggcgaggttt ataaagcaaa agattccttg atgatgacca aacgagcggt tgcgatgacc
     1081 ttggtggaac aagacactct ttatattcac ggcaaacgat tgattgtgac cggaaaaacc
     1141 gaagaacggg tgattagagg ctacaataat gtgcgttttt ataaaaccga tatgagtgga
     1201 aaatgtgact ccctccattc ctctgaaaaa accggtttga caaagctgat aggcaatccg
     1261 gtgatgtgga attatgacaa tcagatgacc ggcgacatca tgcacttgat ttcgaataag
     1321 gaaaccgaaa aattagactc tttgaaagtg ctcaacaaca cgtttattgc ctcgaaggat
     1381 acgattggct ccggctataa tcaggtgaag gggcaaaatt tgtatgggaa attccgcgac
     1441 aataagcttt atgaagtgga tgtggttaaa aataccgaag ttatttatta tatgcggaat
     1501 gacgaacagg aactcattgg catcaataaa agtgccagca gcagcatcaa tatgacctta
     1561 gacgggaatg aaattgaagc cattactttt tttacgaaag tggaaggtga aatttatccc
     1621 gagaaagatt tgcccgaaaa cgccaggaaa ctcaagggat ttatttggcg tggcgatgaa
     1681 cgcatccaaa gcaaagagga cattttccct cccgaagaga atgaatatga tgaaaaaatg
     1741 aaacaggaaa caaaagcgga ggaagaaaag gaagctgttc caatggaggt tcgaaaagaa
     1801 tcgctggaag tggaagagaa gaaaaaagac agttagcatg ttgcgatttg catgttgcga
     1861 gttgaaagtt ttttctgtta cctatttccc attacaaatt accaatcatg aaagaagatt
     1921 tccttaaata tcaagcacaa acgtcgccct acccgcttgc catggaagtt tcctacgcca
     1981 agggttctta tatttttgat acggcgggaa agaaatactt agactttgtt gccggtgttt
     2041 cggcttcgag tttaggccat cagcacccga gggtgaatca agccattaaa gagcaattgg
     2101 ataaatattc gcatgtgatg gtgtatggcg aatatgccca acatcctgcg gttgaatttt
     2161 gtaaattatt agcgaagcac ttgccggagc cgttagacaa aacctattta gtgaattcag
     2221 gcacggaagc tactgaggga gctttgaaat tagcccgccg cgtgacgggc agaagtcatt
     2281 tggtttcgtg tttcaatgcc tatcacggca atacgatggg ttctatgagc gtgatgggat
     2341 ttgaagaacg gaagcaagtt tttcgaccct tagttccgga tgtggatttt attacgttta
     2401 acaacgaagc agatattgta aaaataacga ccaaaacagc gggtgttatt ttagaaagca
     2461 ttcaaggagg agccggattt atagagcctg aaaatggttt tttgagaaaa ataaaagccc
     2521 ggtgtgatga agtgggagcc cttttaatcc tggatgaaat acaacccgga tttggaagaa
     2581 ccggaaagtt atttggattt gaaaattatg aggtggttcc tgatatagtg attatgggca
     2641 aaggaatggg tggcggaatg cccgttgggg cttttactgc caaggcagaa tacatggatt
     2701 tactgagtga tcatccgaaa ttagggcata ttacgacctt tggcggacat cctgtcatag
     2761 cggcagcttg tttagcgacc ttgcgtgagg tgactgaaac cgaccttatt ccgcaaacat
     2821 tagtgaaaga aaggttgttt cgtgaacttt tggtacaccc tttgatacaa gaagttagag
     2881 gaaaaggatt gatgctggcg gcgatggtgg aaacgcctga gattacgaat caggtcattt
     2941 ttaaatgtca agataaagga ttaattttat tttggttact ttttgaaggt agagccatcc
     3001 ggattacgcc accgctcaac atttctgaag aagaaattag agaaggttgt gcgattatgt
     3061 tggaagcgtt agatgaagta atgaaagaaa ccgcagacca atagtaatct ctctccacct
     3121 caaaaagttg ttaattaaat tgtttacaac aatttgacat taaaatagaa aaaagctaat
     3181 ccttcgctat ttttattgaa ggataatttt aaaaaatcga gtatgcattt aagtcacgaa
     3241 gaagacgatt ataacatatc cttatcgaaa tttgaatcga tgctcaaaac caataaagtt
     3301 ttgtttttcg attcagaaga gtttgaagaa attattcttc actacctcga tatgggaaag
     3361 gcatctctgg ccaaaaaagc cctgaaatta gctcttgaac aacatccgaa atcaaccggt
     3421 ttaaaattgg ttcagattga aatgttgatt tatgatgaca agctcgacgt ggctgaaaaa
     3481 atgctgaacg agttgtatgc cattgaacca cacaatgaag agatttatat tcaaaaggcg
     3541 aatatttact ctaaaagaga tcaacacgaa aaagccattg aagagttaga aaaagcatta
     3601 acctttacgg atgattttgc tgatgtttat aacttgatgg ggatggaata tctgttcatg
     3661 gataatttag agttggccaa gtataatttt attaaatgct tagaagttga tattgaagat
     3721 caatctgcct tatataatgt ggtgtattgt tttgagtttt tagaccaaca aaaagaagct
     3781 attgactatt tagaaaagta tattgagaaa aatcccttca gtgaaattgc atggcatcag
     3841 caaggtcgat tgagttatag cattaaaaac tatgagaatg ccttgcgttg ttttgattat
     3901 gccaccctta ttgatgatga attcattgga gcctttatgg aaaaagggaa atcacttgaa
     3961 aaactcaagc gatatgaaga agcgattgag gcatttcaac gaaccattga attagaagat
     4021 cctacctctt atgctttgtt acgcatcggg aaatgttatg aaaaattaaa taacaaagcc
     4081 gaagccatca agtattatga caaaaccgtt catgaagatc ctttattaga caaaggatgg
     4141 attgcgatta ctgattttta tattcgtttg aagaactatc aaaaagcttt gtattatgta
     4201 aacaaagcca ttgcgattga caatgaaaat caattgtatt ggaagcggta tgcaaccatt
     4261 aaccgccaaa tgaattttta tgaagaagcg gaatttggtt accgaaaagc cgtagaattt
     4321 ggtgattatc aattagacac ttggttgttt tgggtagata tcttgcagtt tttaggcgaa
     4381 tttgacagtg ctattcaaac tttgttgcag gcagcagaat atttccctga gcaatatgaa
     4441 gtagaatacc gtttggccgg aatatattat atgttacagc aaaacgaaaa aggtaaaacc
     4501 catttgaata acggattacg cattaatgcc aagaacagaa ccgttttgca agagttgttt
     4561 ccggtggttt gggacaaaaa agttgtacaa aatgttatta caaagcattt aaaatcttag
     4621 cataatttcc aacatactta taaaaatttt ccctaatttg cgttccgttt ccaaaagcgg
     4681 aacgcttttt ttatgagaaa atttttccct gattactttt tgattagcct taaaggatta
     4741 gccatgggtg ctgccgacgt agtgccgggt gtttccggtg gaacaattgc ctttatttct
     4801 ggcatctacc aagaactgat tgacacgata aacaaagtag atttttcttt tttcgcttct
     4861 tggaaaaaac acggatttaa aattgcgtgg caacaaatta atggtagttt tttattggct
     4921 ttactctcgg ggattgcgat tagtattttg actttttcaa aaatcatcac gcatttatta
     4981 gcaacggaac cgatcatggt gtggtccttt ttctttgggt tagtgattgc cagtattttt
     5041 tttgtgggga aacaaattga taaattttca ataggttcaa tttccttttt tctactggga
     5101 acggctttat cttattatat cacgattgca gaaccggtgg cctcacctga tagttatccg
     5161 tatttattct tgtcgggatt tattgcgatt attgcgatga tattaccggg aatctcggga
     5221 gcatttattc tactattaat gggttcttat gcggtggtga ttggtacaat taatcatttt
     5281 agggaaggga ttttatcgtt gaattttcaa gttatttttc aggctgtttt aaaattagga
     5341 acctttgcca tcggtgctat actgggattg aagttatttt caaaagtatt gcattggatg
     5401 tttgacaacc acagaaatag tactttagcc ttgctaacgg gattcatgct cggttcatta
     5461 aataaagttt ggccatggaa agaagtaatt tcaacgcgaa tcgattcaca cggagttgaa
     5521 gtgcctttta tggagaaaag tattttgcca actgctttta atggtgataa tcaattattg
     5581 gttgccatac ttttgatgat tgccggattt ttaactattt ttatcgtaga aaaagtagga
     5641 gcaaaatttt cgaaataaga aaatattttg gctgaagaat gaacaaaaac aacttaaatg
     5701 gatttttttg atttactaat aaaattttta tcgcagccgg atcaaattct ttcaaaacta
     5761 attgaaaaat atgacaattg gatttatgcc attctgtttt tcttagtatt cgccgaaaca
     5821 ggattaattg tgatgtcatt tttaatgccg tttttacccg gagacgctct tatttttgcc
     5881 atagggatga ttgcagcacg cggggaattg gatattgtta tcattgttcc gcttttaatt
     5941 tttgccgctt tactgggtga taatttgaat tattatgtgg gcaagcgatt tggttctttc
     6001 atattaaata gtgaaaaaac atttataatt agaaaaagtc atattctaag agctcaggaa
     6061 ttttttgata aacacggtag aaactcaatt atcatggcac gttttattcc ggttgttcga
     6121 acaatagtac cttttttgag tggagcaaca aatgttccgt ataaaacttt tttaaaattc
     6181 agttttatcg gagcgtttct ttgggttgcc gcaattagtt tgttagggta ttttttaggc
     6241 caaatagatt atgtgaagga acatttagag aagttcatca tcctcatcat aattgtagca
     6301 aacattccgt taatcaaaca gtttttaccc cgaaagaaag caaaaaatga ataagttatt
     6361 tggcctaatc ggaaaaaaca tcagttattc tttttcaaaa aaatacttca cagaaaagtt
     6421 tgaaaaagaa cagctcaccg attgtgctta tgagaatttt gacttgactg ctatttcaca
     6481 atttccaaaa gttatacggg aaaatgcaac attatgcgga ttaaatatta ctattcccta
     6541 caaagaaaaa ataatacctt atttagataa actttctgaa aatgctcaga aaattggggc
     6601 tgtaaattgt attaaattta ctaaaaaagg caaactaaaa ggctacaata ccgactatta
     6661 cggatttaaa aaatcacttc aaccgctatt acaaccccat catcaaaaag cattaatctt
     6721 gggaacgggt ggagcctcaa aagcggtggc ttttgcttta gaggaattag gtattttata
     6781 cacctttgtt tccagaagta aaaaagaaaa tgcattagat tacaaataca tcaatgccac
     6841 cacctttgat aattatcagc tcattataaa ttgtactccg ctgggaactc atccaaacat
     6901 agaagaatgt ccgccaattc cctatgatta ttttactccg gaacacttgg cttatgattt
     6961 gatttataat ccggaggaaa cgctgttttt aaaacaggct aaatcaaaag gtgctgttac
     7021 caaaaacggg tatgaaatgc tcgttttgca agcagaacga gggtggaaaa tttggaataa
     7081 aaagagtatt tctagcttta gcaatacaga tactgccatt taaattagta aaacttcatt
     7141 ttaaatacca taattaaagt tataaatggc ttgattactt tgaattttgc ctagtgttta
     7201 gtatctttcg ccactataat atgaacctta cacattttta caatgttaga agaaaagaat
     7261 gataacctgt cgccaattga aaatgagaca gacggaaacc aagaaaacgt ttcacaaaac
     7321 gatattcaag aagtaattga aattactgat aacgctcaac ccattgtaga tgagccaatt
     7381 gaaactatta aagaaatgag ttctgaaact acagttaatg aggttacgga aacaattgaa
     7441 gcaccagaat cagtagaagt tgatgaagtt attcttgatg caaatgaagt cgtagaagaa
     7501 gcggttgtta caatctcagc tcaagaactt gcagacgata ctccaatgtc agaaaatgga
     7561 catcaaatag ctttgaatgc tattgatgaa tcaaatgcag aagaaagtga agatgaaacg
     7621 ctaaaagaac gtcacaacat acctatgtta gaatatgcct ccttatcgat ggaagcattg
     7681 gttgatgaat tggaaaaact gacagaggtt gaaaaagtga tgtctgtaaa agaacatgta
     7741 gaagaaatta aaaaagaatt tctagcaaaa taccatcatt ttattgacga aaagagagat
     7801 gaatttaaag ctgaaaatcc tgattcaaca gaagattttg agtatcattt gcctttaaaa
     7861 tcaaaatttg ataccttata caagcaatta aaagggaagc aaaatgctca ttttaaaagc
     7921 ttacaaaaca atttaaatgc caatcttgat cgtagactag ccattgtaga ggaaataaaa
     7981 aacctaatca atccgcaaga aaacattaaa gatactttga aaatttttaa tgaattaaga
     8041 gaagaatggc gtacaattgg accgattcca agagacaaat acaatcatgt ttggaataat
     8101 tatcattttc atgttgaaaa tttctatgat tatttacact tagaccgaga ggcaagagat
     8161 ttagatttca aacacaattt ggaacaaaaa caaaaaatta ttgcccgagt tgaagaattg
     8221 gtaaatgaag ttgatattac caaagctttt agagaacttc aagatttaca ccgcatttgg
     8281 aaagaagaaa taggcccggt tgatcgtgaa caccgcgaag aaatttggaa tcagtttagt
     8341 gcgttaacca aacaaatgca cgataagcga gaaggtttat ttgcaaatca acgtgaaaaa
     8401 gaaaccgcta atttagaaaa gaaaaaagaa atcatcgcag aaattgaaaa gctggctacg
     8461 gaagaagtta cagcacattc tcaatggcaa gggttgattg aaaaagtgga agctttacgc
     8521 aatgacttct tttctgccgg taaagttcca tcagaaataa atgaagctac ttgggctgaa
     8581 ttcaaaacag tcgttcgtaa ctttaattcg cataaaaatt ctttttataa agacattaaa
     8641 aaagaacaaa atgacaactt aagcaaaaaa atggctttag ttgaaaaggc aaaaacactg
     8701 agtctcagtg aagattttgc ggccactacc cctttgatga aacaaattca ggaagagtgg
     8761 aaacaaattg ggcatgtgcc aagaaaatat tctgataaag tttggaaaga gtttaaagat
     8821 gcttgtaatc aattttttga cagaatgaaa gcaactcgta atgaggctaa ttctgaagaa
     8881 attgaagcat ttgataagaa aaaagcctat ttagatgcat tgaaagattt ccaattaaca
     8941 ggagaacata aagcagattt agaagctatc aaagctcaca tagagcaatg gaaatcattt
     9001 ggaaaagttc ctttttcaag aagacatgta gaaggaaaat tcaacaaaat attagatgct
     9061 ttatttgaaa aattaagttt gagtaagaaa gatggcgaca tgatgcgttt ttcgaataag
     9121 cttgagcaat tggcaggttc cgatgatcat agaaaactgg aaagcgaaaa aatatttatc
     9181 attagaaaaa ttgaagaatt acaatcggaa attttccaat tagaaaacaa tattcagttt
     9241 tttactcatg ctaaaaaaga taatcctttg gtaaaagaag ttttcaaaaa tattgaaaaa
     9301 cacaaagatg aattgaaaac ttggaaagat aaactgaaac aattgagaag tttaaaacag
     9361 gaataaattt catttagatt aaatataaaa ccatgctcaa atagcatggt tttttttatt
     9421 aaaaaaataa cattttaaag caaacaaaac aatacttttg tgtcgtgaaa tggattaaaa
     9481 atagtgcttt attagccggt ctatttattt tgtttctttt tgggttgcaa tctcatgcaa
     9541 ctcctgaaga aaaaagtagt aacaaagagc agcagataca atcgattgaa tttataaaat
     9601 cggttgggac actacaatct gttggggaaa atcaaattca agttattgtt aaagagaata
     9661 cgacaacaac gtattcgaaa cattttcaat ttttctttga aaaacaagtt acatttttac
     9721 ctactaaatt ccgaactttt ctttcaagac aagatcacaa tcgatgtgaa aaagtctcga
     9781 aaaacctctt tccctttcat tttttctggt aattgaacct ctttgttcga acaaagtcaa
     9841 cttgctttaa gatttcctta aaagcaacta ttcaattata acaaaaaatt taaataaaaa
     9901 tggaaacttc caccacactt attgggttat ttttaatcct acttactgca ttgcctattt
     9961 tcttattact tcgtacgcaa cataaaaata aatctaaaat agaaacaatt ttaaaacaat
    10021 ttaatcaagg aaactcaaaa gaatttaact tgagagaatc attaaacaat aaaataatcg
    10081 catttaatga aatgaagaaa aagcttgttt taattgattt gaatatcaaa ccagaaattg
    10141 ttacttatgt tgatttaaat gaaataagta acagtaaaat tattcgagaa atggaacact
    10201 ttgatggttc taaaagcacg aaagaattta ttaccaaggt tgagatactg ctacataaca
    10261 aaaaaaatca acaggatgaa actttgaaat tctatgatta tgaatatgaa aaacctattc
    10321 aattaaatta ttttagagat aatcaactgg cagaaaaatg gttagagatt attaacaaag
    10381 taatctaatt aaaattaaga gactcttaag gagtctcttt tttttgcatg acaattatca
    10441 tttttatttt aagtcttccc ttataccttt ggatagtatt ttagctttga ttgaaaaaag
    10501 ttaagtggaa atacattcaa aaaatcttaa cttttacagt aaaataaaaa aataatgtca
    10561 cttattcaaa aatataacgt tccggggcca aggtatacaa gctatccaac tgtgccttat
    10621 tgggatgaat ctcatttttc attatcaaaa tggaaagaaa cattacaaca atcattttta
    10681 gagagcaatg ccgctgaagg catcagttta tatattcatt tacccttttg tgaaagttta
    10741 tgcacttttt gcggatgtaa caaacgcatc acaaaacgac atgatgtaga gcatccctac
    10801 attgaagctc ttttaaaaga atgggagtta tattgtgaat tattaccaaa gaaaccaatt
    10861 ataaaagaaa tccatttagg aggtggtacg ccaacttttt tctctcctga aaatttggag
    10921 cgattaataa atggcctgtt tgaaaatgcc acaaagcatg aagaacacga atttagtttt
    10981 gaaggacatc ctaataatac aacaagagca cacttacaaa aattatatga tttaggattt
    11041 cgcagagtga gttttggtgt tcaggattat tcagagacag ttcaaaaggc aatccataga
    11101 ttacaacctt tccacaatgt agcgaaagtt actttttggg caagagaaat tgggtatacg
    11161 tcaattggac acgatttaat ttttggctta ccctttcaga aaattgaaga tgtacttgat
    11221 actattgata agacaaattc cttacagccg gatcgattgg ctttttacag ctatgctcac
    11281 gtgccttgga taaaaggaaa tggccaacgt ggatttaaag atgaagattt accgaaagat
    11341 gatgaaaaaa gattacttta tgaagtcggt aaaaaacatc tttctgaaaa aggctatcat
    11401 gaaattggaa tggaccattt tgctttagcc acagacagtt tatatcattc ttttaaaaat
    11461 ggaaatttac ataggaactt catgggttac acttcttcta aaacacaagt aatgattggg
    11521 ttaggcgttt cttcaattag tgatagttgg tacagttttg cacagaacgt taaaacatta
    11581 gaagagtact atgatctttt agctcaaaat gaattaccaa ttttcagagg acatctatta
    11641 accacagaag acttgattat tcgcaagcac attttgaatt taatgtgcca attccacacc
    11701 acttgggctg aatatggaac ctatttttct gaattaccgg aagttttggg acgattagaa
    11761 gagatgaaaa acgatggttt ggttcaaatc acagaaaatg ccattacagt taccgaaaaa
    11821 ggaaaagcgt ttgttcgcaa tatttgtatg gcatttgatt tacgaatgac ccgaaatgtt
    11881 cccgatacag aattattttc gatgacggta taattaatac ttttttagtt tgattataga
    11941 agatatataa aaacgcccaa attttcattt gggcgttttg tttaaagaaa tatattcaac
    12001 taaaaactat acttccacag cttcttttgc aaaatcagct actaatttat tttgaatttc
    12061 ataaggaaca ctttcataat gatcaaattc catagctatt cttgctcgac cttgcgataa
    12121 agagcgtaaa tcaccggtaa actcgtgcat ttcagcaatt ggtatttgag ctttgattat
    12181 ggtaaaatga ccttctgaat ccattccttc taccatagaa cgatgagatt gaatggtact
    12241 cattatcgaa cctgtaattt cttcaggagc tgtaacggta actttataaa tgggttctaa
    12301 taactgtgga ttggcattaa caaaattttc tttaaaggcc atcattccgg caattttaaa
    12361 ggacaaatca ttactgtcta caggatgcat ttttccatca tacacaatta cacgaacatc
    12421 ctgaacatgt gaacccgtta atggaccttc ttgcattttt tccataactc cttttagaat
    12481 tgaaggatgg aaacgagcat caatagctcc tcccacaata caattgtagt aaactaattt
    12541 tcctccccaa gtcaattcaa tttcttcttt tccacggata gaaactccgg ttggttcagg
    12601 cattccatca taccaaggtt caattttcat ggatatttca ccaaattgtc ctgcacctcc
    12661 tgattgtttt ttatgtctgt aaacggtatc agctccacct tgaatggttt cacggtaggc
    12721 tacttttggt ttttgaaaac gaaccgataa tttatgggca ttttctaatt tccatttgat
    12781 aaccgctaga tgtaattccc cttgacaatg aattaacgtt tgtcttaatt cagcagaaac
    12841 ttctactcta atggatggat cttcttccac taattgatgt aaagcggatg ataatttttc
    12901 ctcttcgcct tttttggttc cttccacagc aattgtgtag gtaggattag ggaactgaat
    12961 tggagtaatt tcaattggaa atcctttttc atgtaaggta ttattggtat gtgtattttt
    13021 caacttttga gttgccccaa tatcaccggc aactaattca tctactggga ttcgtttatt
    13081 tccttccatt tcgaatagtt gagtaatccg ctcaagttgc ccggtatttt cgttagttag
    13141 atcatcccct actttaattt taccggagag tactttgaag aaactcaaat cgccaacgtg
    13201 tggttcagaa actgttttgt aaacaaaaat agctttgttt cctttttcag aacaattaat
    13261 ttcttcaccg gcaattgttt tttgctttgg catttcaaag gcagatggac aaacattatc
    13321 aatgaatccc atcaatctgc cggctcccat attcaatttt gcagaaacac agaatacagg
    13381 aaacaattcg tgtttaatta gggaatgatg caaacctgat ttcatttcat cttcacttaa
    13441 ctctcctttt tcgaaatatt gctccatcag attttcatca tttccggcaa tagtttcaat
    13501 aagttcttga tgcaattggt ttgctttttc tttttcagca tccggaatag ctaatttttc
    13561 gggttttccg ccagctttgg aatatttata catcaccatg ttcaacacat caataataga
    13621 atcaaaattc aacccttgat taacaggata ttgaacaacc actacttggt ttccaaaatg
    13681 ttctttcgct tcagcaatag ttttgtcgaa atctgattta tcattatcta aatgattcac
    13741 ggctaatagc atcggtaact tgtaatcatt ggtgtattgc cagataatat ccgttccaac
    13801 ttcgactccc attcttgaat tgagcagcat caaaccacag tcagctactc gcatcgcact
    13861 gattatttcc ccggataaat catcataacc gggtgtgtct aaaatattaa ttttgaatcc
    13921 tctccattta gaattcatta attttgaaaa aagtgtttta cccttttcat gctccatatc
    13981 ggagtaatcg cctactgtat tttgatcttc aattgaacct cttcggttga taattcctgc
    14041 ttcaaaaagc atagactcag ccaaagtggt cttaccgctt ccggtatgtc ccaacagtac
    14101 tacatttttt acatgattgc tatcgaaatt tgccataata tagtttttta gaattatttc
    14161 aatatcaaat ttcgaaatat attatgagaa taaatatgat aataatcagt taaagggtgt
    14221 ttatgttaaa ctaatttttg attgtccata attactcaaa attgataaaa atcattttat
    14281 ttgaaataaa aagctataaa tttatttagt tttacagtaa attttgagac ttaagaattt
    14341 tatagacttc actttaaaga aaaaagagat aagcagggaa tttaatctgc aactactttt
    14401 tcatacctta ttacatcaca aagaccttca ttaaaacaag aaagtcttag ctcattttct
    14461 gttaacaaag ttattttaga actaccatta ttattaatcc catttgggtt tggattataa
    14521 ttaagattaa gtatattgct attaaccaca gaaaatgttc ctgtatattg atagttttgt
    14581 agtaaatgaa taaaagtatt atcactatta taggtaataa tatctccatt ttcgtaagcc
    14641 atataaagtg gcatgtcatt ttcatcaaag acatcatgta tataaattcc tttgaactgc
    14701 cacgaaccta ttaattcttc ttgaaaggaa gtttcttctg ttgtttcatt ttttgaacaa
    14761 cctattaatg ttgttaccaa taagagtatt aaaatatttt ttttcatgat ttcatgttat
    14821 tattctgcaa ttattactct ttgagaaaaa ccaccttcat tggtaaataa attaacaaca
    14881 taattattcc cactatccaa atgtaaattt ttaatagtaa actcattatt ttcaaaaaca
    14941 ttactataaa tctgcgtgag cagatttcaa aataaactta gctataaata aatgtcaatt
    15001 atgacaatcc ggattagcct gaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnaaaataa acttagctat aaataaatgt caattatgac aatccggatt agcctgaacg
    15181 accaaacttg ttgtgtttgg tgaaatatac ggaatatcaa gtcccatacc tctgacaata
    15241 aataaactcc cgatgagtat tacaacaatg ggtattaatt tttgaatacg gtttcgaact
    15301 ggaactgtaa tgaaattaga aataagtaca acagcactca tcataggaat agtaccaatt
    15361 ccgtataaaa ccatataagc aacacctaaa agctcacttt gcatagcaat agctccgaaa
    15421 agagccgcat aaaccaatcc gcaaggtaga aaaccattta ataatccgat ggtaaataag
    15481 gcatccggag attttcgttt gaactgtgcc cctaaatgac ttttaacttt agaaattaat
    15541 ttataaattg gttttgaaaa attatattga actaattttt tttcaggtac aatcacaaat
    15601 ataatcatca tgattcccac gaaaatagat aatttttgtt ggaatccggc taagaaaagt
    15661 cctttgccaa gaatgccaaa gaacaaaccc aacataccat aagccgttaa tcgacctaaa
    15721 tgatagagta aaatttgaaa aacttttttt gtttgattac ttctatcaat aggaagcatc
    15781 atggcaatcg gaccacacat cccaatgcaa tgaaaactac ttattaatcc aaaaagtaat
    15841 gcggtataaa gcatgaagtt aagggttata ggttaagggc aattaatata cccattacca
    15901 tttaaaaata tattgttttt ttgtttaaaa attctttccc atcataggtc caatctaaag
    15961 aaatgtccca acgaccgcca accaaattac ttttaggtat gagcaaatgt ggaccggata
    16021 aagaaatcgg aacttcaaaa tccagctttt ggttagacgg tctatagagg gacacttttc
    16081 cggtaacttt agttaaatct aaatcggatg gaaaagttat atttattcct tcttctgagg
    16141 tgcttatact aattttttca gtcatatcaa ttgcattttg ctcttttgtc atttggtttt
    16201 caaatttgag ctcttgctta taatattctt ctactactaa ttcattgtca tattggctgt
    16261 ctgattgcac tttgaataca aaatacaaga tgaaagttat gaataaacca aatgcaatga
    16321 caattcctgt tccccaattt attttcattt tttatttttt tttattttac atcggataaa
    16381 gtccgacgaa tgttagctaa agcaaagatt attaattaaa acttcttggt cccataaagt
    16441 ttgtggtggc agtttcaatt aaattttcac catcatatac ttcaattttt aatttaattt
    16501 tatccccttc caaataggcc ggattaattt caataaataa tgttcctttt gcaattccgt
    16561 ctgctttaac tttaacttca gcatttccta ccagttttat tgtcccctta ggttcaatta
    16621 atttaaacgt aacgtgatta aactctttag ttgttttatt tacaatttta taagtataaa
    16681 cattactgat attctctcct ttatgttcaa ataattgtcc aggtaagcgt aaaattgtag
    16741 cttctacatc atttcgtaaa aaaagcattc ctattaaaac accaattaaa atagttaata
    16801 cagcggcata gcctttcata cgagtagtaa actgaaattt ttctttcttt acaatttcat
    16861 cttcactggc atagcgtatt aaccctttag gcaaaccaac actttccata atggtatcac
    16921 attcatcaat acaagcagtg caattgatac attctaattg cgtaccgttt ctaatatcta
    16981 taccggttgg acaaacatgc acacaaagtt tacagtctat gcaatctcct tttccggtag
    17041 atgctctatc ttcttgttta ttaaatttgg cacgacccaa ctctttttca ccacgaacat
    17101 ggtcataaga cacatttatg gatttgttat ctaataaaac tccttgcatt cttccgtagg
    17161 gacaggcaat aatacatact tgctctctaa accaagcaaa aacaaaataa aaaacggcag
    17221 tgaatattaa taatgcaatt aatgtactag tatgtaatgc aggaccttct ttaatcatta
    17281 acaacaaacg gtcactactt actaaatagg ctaaaaaaac attggctatt atgaatgaaa
    17341 tgataaagaa cacaatccat ttagtaactc tttttcgaat cttttctgcg ttccattctt
    17401 gtttttgcaa ccgaatttgt gcacctctat ctccatctat ccaatattca attcttctga
    17461 aaaccatttc cataaaaata gtttgtggac aaaaccaccc acaaaaaatt cgtccgaagg
    17521 ctacggtaaa taaagctaac ccaacaactc caattatcat ggagattaca aagaggtgaa
    17581 aatcctgagg ccaaaaagga aaacttagaa tattaaatcg tctttccaat acattaaaca
    17641 tcaagaactg atgtccattt atttttataa aaggagcaga caataaaaaa gccagaagaa
    17701 aataactcaa tagctttctt ttatcataaa aaggtccttt gggtttcttt ggaaaaatat
    17761 aagaccgttt tccttcttta tctattgttg atatactatc tctaaaacta tcgtctttta
    17821 tatttgacat ttcatttgtt tgttaaattt gcaaatcccg actaaatcgg gatttgcaga
    17881 aaataatttt aattttgagc tacaacttta tcagttgttt ttgttgaatc aatgggagtc
    17941 tcaattgctc cttccgcttt tggagcattt tcatcaaccc aaacatcacc ttcagcagct
    18001 tttgcatcta taggattagt tccttgaagt gaaagcacat aactagctac tttctgtaaa
    18061 tcagaaggtt taattattgc tttccatgat accattcctt taccttcacg accaccttcc
    18121 attaatgtat taaatacatt tttaattcct ccacctaaaa tccaatgttc gtctgttagg
    18181 ttagggccaa tagcaccacc accatcaggt ctgtgacatg ctacgcaatt cgtttcatat
    18241 atttttttac cttctgctaa tgcaccggca tctgtcaata aggtaacttt atctttatct
    18301 aataaatcag gagcagttaa tttatacttt tcaacttcag ctcttgccac tgccatctca
    18361 gtttcaaatt cttcagcttg cgtataatct cccataatat gaaagcgaat agtataaaca
    18421 attgcaaaaa ttatagtaat gtaaaacaat cctacccacc aaggtggtaa aacattatca
    18481 agttctttga taccgtcata atcatggtca agcaaaacat catgttcttg ttccatttcc
    18541 ttagaacggg ttaatttttg aactattccc tgatagaact tgccttcggt aaatggcaaa
    18601 gattgagctt cttctaattg ttttttctgt tcatctgtca ataaatgata agttacttta
    18661 tccacagcac taacaacaat ttcaatggct actaataaga ataaaaatac tcctaaaaat
    18721 aaagcaacca ttgggaattt aataaaggca ggttgattac ctgaatcgat aaaatattcc
    18781 attgccccca ttacgatggc gaaaatcaat ggaactctaa cgtatgatgg aattaatttt
    18841 ttcataagta tgatgtttat tttggtattt aattatcctt aaaaggaagt tcgcttaatt
    18901 ctttgattgt ttcttttttg tatgtcattg cataaataaa ataagcaaca aaaactacaa
    18961 agaagataga aagagagatt atcgggtata tttcaatacc gtcactatcc atagtatgtt
    19021 taacaaactt tagcataact tctatttatt ttgtgcagtt tctttaactt tcaaatcttt
    19081 acctaaacgt tgtaaataag caattaagga tacaatttct ctatttttca ttggtacaaa
    19141 ttcttctcct cttgttgcag cagctttttt actttgctca tatgatttca caaaatcagg
    19201 atctgaaaat aaatttttct caatcgctga ggcttgttcg tccatatgtt ttctagcatt
    19261 tttaatttca tcatcagtat aaggaacacc taatgtaacc attacctcca ttttcttttc
    19321 aatataagaa acatccattg ctttattatc atacatccat ttgtaacccg gcataattga
    19381 acctggtgat gtactttgag gatcccacat gtgattaaaa tgccaattgt cattgtattt
    19441 acctccaact cttttcaaat ccggacctgt acgttttgaa ccccatagga atggatgatc
    19501 ataaacatat tctccagatt ttgaatattc tccatatctc tccacttctg aacggaatgg
    19561 acgtatcatt tgtgagtgac aattattaca accttcacga atgtataaat ctctaccttg
    19621 taattctagc ggtgtgtaag gttttactgc ggcaatagta ggtatattag attttacaac
    19681 taacatcgga acaatttgaa cgataccacc aatagcaaca gcaactgtgg ttaatagggt
    19741 aaattgaatt ggttttcttt ctaaccaagc atgccatctt tcacctttaa cttgatgagg
    19801 acttatttta gttaaagcag gtgcttctgc caattcattt tcaaccggtg aaccagccat
    19861 aactgtgcgt gccacattat aaactaaaac aataattcca acaaaatata aggtaccacc
    19921 aattgctctc atccaataca ttggcataat ctgcgtcaca gtttcaagga agttaccata
    19981 tactaatgtt ccatccgggt taaattgttt ccacatagaa gcttgtgtaa aaccagcaac
    20041 atacattgga agtgtatata aaataatacc taatgttcca atccaaaaat ggaaattggc
    20101 taattttgta gaatatagat ttgtttttgt taatcttggg aataaccaat aaatcatacc
    20161 gaacatgata aatccattcc atgctaatgt tccaacgtga acgtgagcaa caatccaatc
    20221 cgtaaagtgt gcaatagcat ttacattttt taaggaaagc attggtcctt caaaagtaga
    20281 cataccataa cctgtaattg caactacgta aaattttaat acaggttccg ttctaacttt
    20341 atcccaaacc ccacgaagtg ttaatagacc attaatcata ccaccccaag acggagcaat
    20401 taacatcaca gagaatacaa ctcctaaatt ctgagcccag tccggtaaag aagagtataa
    20461 taaatggtga ggaccagccc aaatgtagat gaaaatcaac gaccaaaagt gaataattga
    20521 caaccgatag gaataaacag gacgattagc cgcttttgga acaaaataat acattaaacc
    20581 taaaattgga gttgtaagga aaaatgctac ggcattatgt ccgtaccacc attgtactaa
    20641 agcatcttga acaccagcgt aaacagaata actttttaaa gcagatactg gaatttcaag
    20701 attattaaaa atgtgtaata atgcaatcgc aataaaagtt gctaaataaa accaaatggc
    20761 aacataaata tgacgttctc ttcttttgat aattgtacca atcatattta taccaaatgc
    20821 aacccaaaca attgcaattg caatgtcaaa aggccattct aattcggcat attctttcga
    20881 agaagtaaat cctaaaggta atgttattgc cgcacctaaa atgattaact gccaacccca
    20941 aaaattaaat ttacttaaaa aatcactgta cattcttgct ttaagtagtc gttgcaaaga
    21001 gtaataaact cctgcaaata ccccattacc aacgaaagca aaaataactg cattggtgtg
    21061 taaaggtctc aaacgtccat aacttaacca cggaattccg tctgtaagat taggaaataa
    21121 atataagaaa gctactaaaa gccctactaa cattcctatt acaccaaaag cgattgtggc
    21181 ataaagaaag tttcttacaa ttttgttgtc gtaataaaat tgttgcttct ccataattaa
    21241 acttgttttt cttctaatgt ttgtatttga tttttgtttg gatttttgtt tggatttttt
    21301 ttcacttcat cgtcaaaaag cattctgacg gatggtgtat aatcatcatc atattgccca
    21361 cttttaactg ccttgataaa agctatcaaa aaaattaaag ccaccacaat actgacggaa
    21421 attaataggt aaattacact catattttaa atttcgttaa caaaagtagt tttcttggtt
    21481 tttatataat atgatatttg tcatatctta aatttttcat aaaattattt aatccgtgaa
    21541 taaacattgg taacaattgt tacaaaacta actattgtta ttgtgctcaa aggcataatt
    21601 atagcggcaa caattggaga gagatttcca gtgacagcaa aactcaatcc gataaaatta
    21661 taaattaaag acactccaaa actcatgtat atcgttttca tggcattttt tgaatactgc
    21721 ataaaaaagc ctattttttc aaattgagat gcatctaaaa taccatcaca agccggagaa
    21781 aaaacattta cattttcaga aatagaaatc cccacattac tttgtgctaa agcaccggca
    21841 tcatttaaac cgtctccaac catcattaca ttacaaccct ctttttgaag attttttata
    21901 aagttaagct tttgttccgg tttttgatta aaaacaatcg tagaattttt aggcaacatt
    21961 ttttctaata ttttacgttc tccatcgtta tcaccagata atactactaa atcatatttt
    22021 ttagctaagt tctcaaataa aaattctaac ccttctctat attgattatt aaaaatatag
    22081 cttcccttat atgcaccatt tatttcaaca tgaactttgg ttttcttatg ggtattttca
    22141 ctcatatgtg caagaaattg agatgaacct aattttatcg tcgtatcccc aaatttagca
    22201 aaaattcctt ttcctataat ttcttgaaat tcattttgtt ccattctttc ttgatttgga
    22261 ataaaatcat acaatctcct acttaaagga tgattggaac ctcttaatgt atttttcaat
    22321 aattttaatt cgtctgaagt aaattgagca ccttcataag taatggaagt ctttttattg
    22381 gttgttatgg ttcctgtttt atcaaataca atcgtatcca ctttggctaa ttgttcaatc
    22441 accgttgcat ttttaagata taattttcgt ttacccatta tacgtaatac atttcccaag
    22501 gtgaagggag ccgttagagc taaagcacaa ggacaagcaa caattaaaat ggcggtaaac
    22561 acattgaaag cagttgtcac atcaataaaa acccaatata aaaaagaaac aatcgctaat
    22621 aacaataaag cgggagtaaa atagcgactg attttgtcgg tgaccgtttt gtgtttgatt
    22681 tcaactttct tttggaatac atcgttgctc cacaattgcg ttagataact ttgagaaaca
    22741 gaaaaaagga cttccatttc aatgactttt cccatttgct ttcctcctgc aaaaatttta
    22801 tcgcccgatt ttttttcaat ggcaatagct tcaccggtta caaaactgta atcgatagaa
    22861 gctttttctg atattaaaat accatcaacg ggtattagtt cctgattccg aattaaaagt
    22921 cgttcccctt tttcaatatc ataaacttga accgaagttt cggttccatc cgataaaatc
    22981 tttgtaattg caataggaaa ataagattta taatcgcgtt caaaggataa aaaatcatag
    23041 gtttttttct gaaataattt ccccaaaagc ataaagaaaa tcaaaccggt catgctgtca
    23101 aaaaatcctt gtccgtaacc aaaaacgata tctacagtgc ttctaacaaa catcactaca
    23161 atccctaatg caatcggaat atcaatattt aataaacctg attttatgct tttccatgct
    23221 gaaacataat aaccggaagc tgaatataaa aaggaaggaa gtgacaaagc aaaaatcaac
    23281 caacgaaaaa atcccctata ttggttaatc caaaattctt ccacttcaaa atattccgga
    23341 aaagagagca acatgatatt tccgaaacag aaaaaagcaa tacctatttt ataaattaaa
    23401 ctgcgatcaa caagtttttt accttgttca taattttcaa gactgatata aggctcatag
    23461 ccaattgagc agagtaattc aactattttt ttaagtgtcg ttttttcagg attgaatgta
    23521 actcttactt tcttttcagg aaaattaacc tgggaggtac taattcctga ttgaagtttt
    23581 tgtagatttt ccaaaatcca aatacacgaa ctacaatgta tatgaggaat ataaagtgaa
    23641 acaatatgtg ttgtatcctc ttggaattcg agaagttttg caacaatttc ttcattgtct
    23701 aaaaaatcat atttaccttg aatgtctagt ggcgttgcac ccggagctgc ctgaaaatca
    23761 taataacacg ttaaatcatt ttgacttaaa atttcataaa ccgtcttaca accgttacaa
    23821 cagaaacttt tctcatcaaa aataatctct tcttttttta ttatttcatt accacaatgg
    23881 aaacaatttc caatgtccat aatttgctca atttacctat tgcaaatatg aaaaaaggtt
    23941 gaaattaaaa acatgataat tgtcatattt aattatcttt gtaaaaccaa ataattaata
    24001 cacgatgagc aagtgtgaac attgtatcgt cagagaattc agttcgctga aagctttgaa
    24061 taaagatgaa ctactcaaaa ttgcaagttg caaaacttca tatactatca aaaaagggga
    24121 acctattttt gaagaaggcg aatctgtgaa tggcatatat tgtataaaag atggtgtttg
    24181 taaactttct aaactaagtg caaacggaaa agaccaaatt gtaaaattgg ttaaatcagg
    24241 cgaattatta ggacagcgtt cgatgattag tgacgaacca gccaacttaa gtgcagttgc
    24301 cttagaagac atggaagttt gttttattcc gagaagtgaa gttttacagt tttttaataa
    24361 caacaaccaa ttttcgatga acattatgaa gtccatttgt ggtgatttaa aagaatctga
    24421 tgaccacatg gtttctatgg ctcaaaaaac ggtaaaagaa cgtttagctg aaactttact
    24481 ctatttagaa gatacctttg ggaaaaatga agatggctcg cttcatattc aactttccag
    24541 agaggaatta gcgggtatga ttggtactgc aacagaaagt tgtattcgat tattgtcgga
    24601 atttaataaa ggaggagtaa ttgatttgaa tggaaaaaga atcagtataa ctgaccgaaa
    24661 taaattgaaa agattgtcgg aataaataag cccattcgta ttgtgagcga atgggctttt
    24721 ttttcaactt taaataatat attaatttct aactaacttt atggtttgaa ctgcagtgtt
    24781 tgttttcact ttcgcaaaat agattccaga agttaaagca gttgcatcaa tagttgccaa
    24841 attacttgaa atcaatttat caactattat ttttcctgtt acatcaacaa tttgtatttt
    24901 ttcaattgaa tcattttcaa ttgaaaaatt ccacacattt tgagacggat ttggataaac
    24961 ttttgcttta gaagttgtaa aatcgcccac actaagtgtt gcaaaattat actctccggt
    25021 taatcgattg aaagttacat tatatgttcc ggcaacggtt ggaatat
//
LOCUS       DAOTCW010000017        24527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1046_length_24527_cov_48.153155, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000017 DAOTCW010000000
VERSION     DAOTCW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 24527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24527
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1046_length_24527_cov_48.153155"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="P5335_02530"
     CDS             complement(<1..420)
                     /locus_tag="P5335_02530"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRZ73789.1"
                     /translation="MKKVSLIFLSIFALFISCDEDDDTTFVQENYVLGKWYVNQIGTI
                     NANNTIVYEDYINATDCEDDNLVLNENKTFEENDFEFINSSCQNFQTTGSYEVENNKI
                     VLTYVDSEGVSVEHVLTIVTLTYEEINLSYTDTETNEI"
     gene            complement(435..1784)
                     /locus_tag="P5335_02535"
     CDS             complement(435..1784)
                     /locus_tag="P5335_02535"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HRZ73790.1"
                     /translation="MKTNFLKSILFIALAAGFTTSCVNDDDYAVPTLECVDSTLQKTK
                     EVAQIPASTDISQYLADDIIEAYVTSSDEGGNFYKSISMQTLDGSKAFSVPVDVTSTF
                     INFEPGRKVFIKMKDLYTDVSNGGMRIGGIYVNSSNVPSVGRLSESLYKTVLSRSCTI
                     VPESDLVKTVTIAQAKNDAVINTLIDIDDVQFTDASITTTYYDPNNDTGGATNHFLVD
                     ADGNSVIFRTSSFANFAGKPVATGRGKVRGVMTKYNSDYQFVARTERDIQLTGERLAP
                     LFSESFEGITSTGNNQFIALPGWSNVSINAGAEKWEARIFSLNKYAQMSAFGTGENNV
                     DTRLITPAINLDNTTAEFLRFGYKTGFANGIALSVWYSTDYDGSGTAAAINAATWTQF
                     SVDLNVQDTSYASNFYSSGNIDMSGINGTVYVAFRYEGSAAGVTTTYQVDNIEVFGE"
     gene            complement(1795..4674)
                     /locus_tag="P5335_02540"
     CDS             complement(1795..4674)
                     /locus_tag="P5335_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ73791.1"
                     /translation="MKKLVFSILLMMQVLVVFGQQTSSITGKIVDSKTQKPLQSIVVA
                     VQNTTLIKLTDAQGVFSIENVPVGEHLVLVRSTGYLQQLLSVDVVAGQTLDLGVIALE
                     EDITTEQQLSLVTITESDLGDDNSGSDNTAGLLQATRDVFQQASAFNWGQARFRIRGL
                     DNDYSTTMINGVIMNKIYDNRPQWGNWGGLNDATRNQDFTMGSAASDHTFGGILGTQQ
                     IYTRASIYRPGTRLSFSGTNTNYSWRMMGTYASGMDKDGWAFVISASRRFAQEGYFEG
                     TDYSANSVFASIEKKINNSHSLNFTSIYAQNSRGKNSPNTNEVTSIAGEKYNSYWGYQ
                     DGEKRNSRDKDLEEPIFMLSHYWNISPKTKLNSNVTYQFGSIANSRLDFQDVTNPDPT
                     YYRNLPSYFTSLYNNNPDEVMQQDPAAYQPGGLGGIFTPDLVGATNANFFNQRQINWD
                     SMYLANSIPIVDENNQEIGREKARSKYVLYQDRTDDKTWTFNTILNSQLSDNIGLNGG
                     ASYTRLNSNNYQYLEDLLGGLYFDDIDNFQDGDASQSDLNNPDRKVVEGDKYGYNYNL
                     YANVLRAFTQFKFTFKKIDFYVAQEFSRSEYQREGLYKNGIYPTNSYGDSEKIAFDNF
                     GFKGGLTYKLTGRHFINFNGVYMSKAPNLRSTFPNARLSNFVFDGLDSETISSADLSY
                     IIRTPKLKAKITGFYSKIQNITETSFFFAEGAFEDTEGDGGDAFVAELVTGMDKLQFG
                     GEFGLEYQITSTIKVNGAAAYGQYTYANNPNVYLNVDANAVNNLPPLVNLGESKLKDY
                     KIPGSPQQAYSLGMEYRDPKFWWIGANVNYLADNYLDVSALMRTDNFYRNPADINGYP
                     FPEATEERARELLKQEKFDSFTLVNLVGGKSWRISNKNRNTVGFFASINNIFDVTYKT
                     GGFEQARNANYRELNQDVSSGTPSFAPKYFYGYGRTYFVNFYINL"
     gene            4810..>5111
                     /locus_tag="P5335_02545"
     CDS             4810..>5111
                     /locus_tag="P5335_02545"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRZ73792.1"
                     /translation="MKIKQLIAVFVVIFAINSVNAQDKKYKVHTVAFYNLENLFDTKD
                     DPNVFDEEYTPAQGWTSDKYQQKLKNLERVLSEIAVNEKQANSPTIIGVCEIENRGV"
     assembly_gap    5112..5211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5212..6008
                     /locus_tag="P5335_02550"
     CDS             <5212..6008
                     /locus_tag="P5335_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRZ73793.1"
                     /translation="QANSPTIIGVCEIENRGVLEDLIKTPKLLTKDYGIIHYDSPDAR
                     GIDVGFLYQKKHFTPTSSANIPLIITNFDGSGKRVYTRDQLLVTGYLDGEEMHFIVNH
                     WPSRSGGEKRSSPYREAAGALNRKIMDSLLKINPNAKIITMGDLNDGSYNKSVKKEIG
                     AKLKKTELKEPGDIFNPFEQMAKDGNASLFYRDSGDIFDQIMISQQFVLEDFSTYQYW
                     KAGIYNKPYMIQQTGQYKGYPLRNSPSEPGFSDHFPVYIYLVKEYK"
     gene            6073..6609
                     /locus_tag="P5335_02555"
     CDS             6073..6609
                     /locus_tag="P5335_02555"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HRZ73794.1"
                     /translation="MAIQKPFNLTKWIEKNRHLLKPPVGNKNIYVDSGDYIVMIVGGP
                     NARKDYHYNETEELFYQLEGKAKVIIQENGERKEFKLNAGDMYLHPAKTPHSPMRSKN
                     SIGLVIERKRAGKGFTDGLLWFCENCNHKLHEVYFELFDIENDFLPHFKHFYNSEELR
                     TCKKCGTVMETDSRFVAK"
     gene            6750..>7862
                     /locus_tag="P5335_02560"
     CDS             6750..>7862
                     /locus_tag="P5335_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRZ73795.1"
                     /translation="MSTIAQQFGMTEALAILGVKAINEGTSTGNHHFSNGEILESYSP
                     VDGQLIGKVKTTTAADYEKVMQTATAAFKTFRAMPAPQRGEIVRQFGNKLRELKEPLG
                     KLVSYEMGKSLQEGYGEVQEMIDICDFAVGLSRQLNGQTIPSERPGHVMREQWHPIGV
                     VGIISAFNFPVAVWSWNTALAWICGDVCVWKASEKAPLCSIACQNIIAGVLKDNNLPE
                     GISCIINGDYKVGEMMTTDTRIPLISATGSTRMGRIVGTTVAQRFGKSLLELGGNNAI
                     IITPTADLKVVVPGAVFGAVGTAGQRCTSTRRLIIHESVYDKVRDAIVGAYEQLTIGN
                     PLDQKNHIGPLIDTDAVNTYLAAIEKAKAEGGKVLVD"
     assembly_gap    7863..7962
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7963..8336
                     /locus_tag="P5335_02565"
     CDS             <7963..8336
                     /locus_tag="P5335_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRZ73796.1"
                     /translation="NHFEIVQHETFAPILYLMKYSGEVENAIELQNGVAQGLSSAIMT
                     NELKEAEKFLSFSGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDSWKVYMRR
                     QTNTINYSDQLPLAQGIKFDL"
     gene            8514..8888
                     /locus_tag="P5335_02570"
     CDS             8514..8888
                     /locus_tag="P5335_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73797.1"
                     /translation="MKKIILVLTCVPFLLATTCEDDDDQIYCTQEAKAALNVSVSLGA
                     MSSITSEGVTVVATDGNYSETLVVVNENDPIFSGAYEREGSYTITVSKEGYQTQTSEI
                     ITVTRDVCHVIPQFIHVVLEPN"
     gene            9149..10939
                     /locus_tag="P5335_02575"
     CDS             9149..10939
                     /locus_tag="P5335_02575"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRZ73798.1"
                     /translation="MKRKLISTTALLLLVQFAICQTSSNLPEKKRPNIVVILADDMGY
                     SDIGMFGSEIKTPNLDALASNGVRFTNFYTHASCSPSRSMLLSGVDTHLNGLGNMDEW
                     TAPNQVGVEGYEGNLNNKVVTVPQLLKDAGYHTYMVGKWHLGKSPDKIPAARGFERDF
                     ALLDGMGSYWDMTNMTALVPNSLFTEDGKYLTKLPKNYYATKTYTEKIISFIDADHKD
                     GKPFFAYVAHQAPHDPYHLPKDWTNRHVGQYDIGWDNIRQQRLKRQKELGIVPESASL
                     AERMWFIPDYSILAPATRAVLGRKMELYAGLVENLDFHIGQLVDHLKKIGEYENTIFI
                     ILGDNGAEGNDLGEMIAGQKGSLNSLFFASKWSNNNINAWGEPGSYVGYGPGWAQASM
                     TPFGQYKGWLGEGGIRNALIVSGPVNRREKGSINNGLMSIADIMPTLLDVAGTTYPKN
                     YNGIQLPPMIGKSWTNVLAGVADSPRTNSDYIAWELFGNRALRQGDWKLRWEYAPFGK
                     GDWELFNVAQDPSERNDLAANNPIKLKEMLLLWEEYVAKNNVILPSRSPFEGVYDVLP
                     ARFPIEEGFPPLIYKRQYTPPKELMNTSKQ"
     gene            10944..>11545
                     /locus_tag="P5335_02580"
     CDS             10944..>11545
                     /locus_tag="P5335_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRZ73799.1"
                     /translation="MKNNLTALSFNFTKKLYLLTIFLLLGCTIVIQAQGDGPRFYWKT
                     LMGTNAVPVITSSMSGNSNPFDPSRFVIPGSNFEATMTMAGYAKMLPLFKRSAMVSVV
                     MPMGRLTSDVSINGLDFSNTARGFGDPMLMLSVNLIGAKPQMNIPDALRYQPKFSLDV
                     LASFAVPIGEYDSSVPINLGQNRWYGRIGTPIVWQIGPWVP"
     assembly_gap    11546..11645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11646..12206
                     /locus_tag="P5335_02585"
     CDS             <11646..12206
                     /locus_tag="P5335_02585"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRZ73800.1"
                     /translation="YREGSFVCQMDYSWGKPKRIIFMRMLNEEEAERIKNKMIRGGYL
                     TGVSLDDKGQTYSENTAFTEFHYRIPVKGSFLITHRLRADLRWMGNENEFSQRYRYRF
                     MIEKELKAKKVSFVPYINAETFYDSRFNTINRFRGIAGTSVSWSKHFAIEGNFTYQYD
                     SKSSVTNLYATNLILHIYFETKAALN"
     gene            complement(12207..12779)
                     /locus_tag="P5335_02590"
     CDS             complement(12207..12779)
                     /locus_tag="P5335_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HRZ73801.1"
                     /translation="MSIIEKRLQDRSHSKCEISGIEDDLVVYTVAPKTEESLENSILI
                     TKALKDQIENPETTNAADWRGLNDSMWNENLPVQIVSWRMLARLKNHDLLEMMYLDED
                     ALAWAKSTNEGDEEEGKIIHKDSNGNILKDGDSVVLIKDLDVKGANFTAKRGAAVHNI
                     KLVWDDANLIEGRVENQTIYILTQYVKKTK"
     gene            complement(12792..13277)
                     /locus_tag="P5335_02595"
     CDS             complement(12792..13277)
                     /locus_tag="P5335_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73802.1"
                     /translation="MKKLFTIGLILSAFLANAQAYSGKGDQKFQVGANFQDHASGITL
                     FYDRGIGENMSIGLQTVYLLGVDGDASKFEDEFDIKARFSANIGSVMKLPEAIDIYPG
                     LNIGLRNFGAHAGIRYFFSDGFGVFSEVGFPIAKYNSDPVGYEQLNNQFQISVGASFN
                     L"
     gene            complement(13421..14149)
                     /locus_tag="P5335_02600"
     CDS             complement(13421..14149)
                     /locus_tag="P5335_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRZ73803.1"
                     /translation="MTNTFVIGDIHGGLRALHQVLERSQVTREDTLIFLGDFVDGWSE
                     SPQVLDFLIELDKKQTCIFIRGNHDDLLLNYLKTNSYHQEWFKHGGQSTIDCYQKVSA
                     VERKRHIEFLEGLKDYYLDDQNRLFIHAGFTNMNGIDFEYFPKLFYWDRTLWETALSL
                     NPTLSKDSIFYPKRLKLYDEIYIGHTPTTRINETIPTNKACIWNIDTGAAFVGPLTIL
                     NVKTKEYWQSEPLPQLYPNEKGRN"
     gene            complement(14142..15293)
                     /locus_tag="P5335_02605"
     CDS             complement(14142..15293)
                     /locus_tag="P5335_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRZ73804.1"
                     /translation="MINKRLLIKNLLAHNDESSFYDKKRQLNLHSKEGKAKFLKHICA
                     LSNSNPTNNSYIVVGVEDQDNEITGDDFFDDSRIQNLVNAYLENPPKIQYENVPFPNL
                     PKDKVVGLVTIKANSKVSFFKKGIHTTPARTVFIRRGSNSIPTEDKVPFSKLNTETVI
                     GIENNSRNSISHTLEGVLDFLNNRHKDMATNYHVFKELFVVCWAGHKKKVRDKTFFSR
                     VDIELINEQVKLFYSAFDEVTIEYDDKTFSITEYVPLGLNDKTSYYPLEKQTLTFFDN
                     GYYKIETFFLFEPPQYNRKMMFHIYNSNLSLLQKLQKGISLSEREKRDLLNLPSTLMI
                     CFLNGFEDAKQKLIDAKPLLKALENPSVYVNFKETLRILRKMKYDKNND"
     gene            complement(15384..16253)
                     /locus_tag="P5335_02610"
     CDS             complement(15384..16253)
                     /locus_tag="P5335_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRZ73805.1"
                     /translation="MKTNLSTVIAGTMNWGVWDKNLSSDEMANLIHVCLENKITTFDH
                     ADIYGGYTTESTFGIGLKKSKLDRSKIQLISKCGIQHVCDTRNNRIKHYDYSKEYIFW
                     SVENSLKNLQTDYLDVFLLHRPSPLMVADEIAEAVEQLKKDGKILDFGLSNFTPSQTD
                     LIQQKTKVSYNQIQFSATDFQPMLDGTLDHMQFHQIRPMAWNPLGTVFREDTDQTRRL
                     KKLFARLIEKYQVGSDLLLLAWIMQHPSKVIPVAGTVNVARIQQLQKAAGLILDKEDW
                     FEIWTESIGNKVP"
     gene            16496..>16827
                     /locus_tag="P5335_02615"
     CDS             16496..>16827
                     /locus_tag="P5335_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRZ73806.1"
                     /translation="MEENTTTLDIRAINEKIERESAFIDLLTYEMNKVIVGQKHMIER
                     LLIGLLGQGHILLEGVPGLAKTLAINTLSQAVQGSFSRIQFTPDLLPADVVGTMIYNI
                     KQNEFSIK"
     assembly_gap    16828..16927
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16928..17371
                     /locus_tag="P5335_02620"
     CDS             <16928..17371
                     /locus_tag="P5335_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRZ73807.1"
                     /translation="IDYPKMEDERMVIRQNLKGSHEKVNAVISIEQILRAQEAVREVY
                     MDEKIEKYILDIIFATRFPEKYKLESLKPLISFGSSPRGSINLATAAKCYAFIKRRGY
                     VIPEDVRAVVHDVLRHRIGITYEAEAENITSVEIINKIVNEIEVP"
     gene            17455..18321
                     /locus_tag="P5335_02625"
     CDS             17455..18321
                     /locus_tag="P5335_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRZ73808.1"
                     /translation="METKDLLKKVRKIEIKTRRLSDHIFSGEYHTSFKGRGMTFSEVR
                     QYQFGDDVRAIDWNVTARYNEPYIKVFEEERELTMMLMVDISGSESFGTKNQLKSEIV
                     TEIAATMAFSATQNNDKIGLILFSDEIELYIPPKKGKSHVLRIIRELIEFQPKSNKTN
                     ISNALKFLSSVMKKKAIVFFISDFITEDNYEHTLKIAGKKHDITGIRVFDIREEKMPN
                     IGMVNMLDAETGETMLVNTNSKSIRMEYEKYYHEKVIFFKETFSKCGSGTVNTRVDES
                     YVTKLLSYFKSR"
     gene            18371..19981
                     /locus_tag="P5335_02630"
     CDS             18371..19981
                     /locus_tag="P5335_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73809.1"
                     /translation="MKIKLYIFFILFSTLVFAQQKRVTTSVDSTKVKIGAQINLTLKT
                     TVDTLSKVTFPEGKLFGRLEVLESYPTDTIKENFKYQLIKKYGLTQFDSGKFVIPSLQ
                     VIINSKPYPTDSVLVEISNVVVDTLKQQMYDIKDITQVDNDGRPWWVYLLILVVLAGI
                     GYLIYYLIQKNKKPKVEEIVYASPIEKATAHLKTLEKKSLVERGEVKAYYSELTDIVR
                     TYIEEAIEIPAMESTTDELIAAFKIAIVKKKLALNDETIKNLERVLKQADLVKFAKSK
                     PLEFEIVEDKSKIEKTIYKIHQSIPEVVEEELIDNDFNKDKILKRKRKRKITTLVLSF
                     IVLLFVVTGILIVTKGFEFVKDTIIGHPTKELLEGEWVLSEYGNPPIKIETPKVLKRM
                     DVSKYMNKESASLMKDMQMFGYGAILDHYYIVIMTNKFKEEIKIDLAKAMEGSLKIME
                     AQGAQNILLKQEEFSSKEGFNGLRGYGTMTIIDPITKKSRKAYYEMIYFSQEGGLQQV
                     MVLQDEGDESANQITERIMNSVEIAKMK"
     gene            19984..20985
                     /locus_tag="P5335_02635"
     CDS             19984..20985
                     /locus_tag="P5335_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRZ73810.1"
                     /translation="MENITFLHPQFFWLFTLLPVAIAWYIWKRNKQTATLKVSSLKGF
                     KVKSSVLPKLKPILFVLRILVLSFLIVALARPRTVDISNKTKTTRGIDIVMATDVSGS
                     MLAKDLKPNRLESLKKVAANFVNDRPNDRFGIVVYAAESYTKSPVTSDKSVVINTIKS
                     IQYDNIIKDGTGIGMGLATAVNRLKDSKAKSRVIILLTDGVNNAGFIEPETAADIARE
                     YGIKVYTIGLGTNGMAEFPYAYAPNGGFLFRMMPVEIDEALMKSIAKKTDGKYFRATS
                     NSKLKEIYEEINKLETTEIEEQKFYNFDEKFRPFVWIAGLLLLVEILLRQTIFRGFI"
     gene            20987..22027
                     /locus_tag="P5335_02640"
     CDS             20987..22027
                     /locus_tag="P5335_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRZ73811.1"
                     /translation="MYELDEKKYLYFLAIIPIIVLLFLFNLYWKRKKQREFGDIELVK
                     KLSPDKSIFKPILKIVVLLFAFVCIVIGLVNPKIGTKLETVKREGIDVVFAIDVSKSM
                     LAEDVAPNRLDKSKQIVSQIINQLGSDRIGIVAYAGSAFPVLPITTDYGVAKMYLQSM
                     NTDMVSSQGTSLEDAIRLSANYFDKGNSTNKLIVLISDGEDHSEGFEGAIEEAKKIKA
                     KIITIGVGTEKGGPIPLKRNGVVESFKRDQNDEMVVTKLNTEVLKQIGKGTKGGYVYG
                     SNTKEVLEYVKKALDNIEKTEFETTQIADFQSQFQWFLGFAFLLLMIDVFLLERKTGW
                     INKLNLFNEKKA"
     gene            22024..22770
                     /locus_tag="P5335_02645"
     CDS             22024..22770
                     /locus_tag="P5335_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRZ73812.1"
                     /translation="MKQNLTILLLMLSYFLQAQEEKKEKDKVLPKANKEFVAKNFTEA
                     EAQYRVSNSKGVSGATPAYNLGNSIYKQNQPSEAKYAYAKAIEKAKTKPEKHKAFHNL
                     GNVFMKEKQYAKAVESYKNALRNNPTDEETRYNYALAKLYLKNNPEDPEKDKKDDKKD
                     DKNQDKKDDGDKDKKEDKKDGDKDKNQDKNDRNKDQDQKSQPKPQPSGISKESVQNLL
                     DAVNNEEKKVQEKVNAQKVKTKPVQTEKDW"
     gene            22776..>24527
                     /locus_tag="P5335_02650"
     CDS             22776..>24527
                     /locus_tag="P5335_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRZ73813.1"
                     /translation="MKKFLFIVFLSCQAIFAQVQFEAKVSKNSLGLNERLRVDFTMNV
                     DGDNFVPPTFDGFRVIAGPSQQVSQSWVNGKSSFNKTYSYFLLPTQKGTISIKQAAIE
                     HNGQVYKTTPISVKVSDAVEQPRDPNDNSVSINDALYLVADVSKTNPYLNEPITVVYK
                     LYFSHNIGITNWQELDKPKYNNFWSQNIDIKQLVAEEGTFKGERFRYVVLRKTVLYPQ
                     KTGSLEIEPLSLDIDVELPTNRRNIFGQVMLTKDHKRVSAGSKIISVKPLPENGKPID
                     YSGAVGKFDFKVIPSRTNLKNGESMDLEVSISGNGNLKLFSLPKPVVPTALEMYDPVH
                     TENVNTPLSGMSGKITDKYTIIPQFKGSYTIKPLSFSYFDLSTGKYKTITSEEIIINV
                     TDGPSQNGNLTAKTTESVDKKAISAGDQFKFIHLKTKFSSINKQDFFGSNLYYVLVSI
                     PFLLIPFIVLFKKKKEAIDGDVIGNKLKLNNRLAKKYLSEAKKQLGNKEPFYVALEKS
                     LHNFLKAKLKIETSEMSKDIIKETLANKKAQTETIEHFIQLTENCELARYAPSSTVAM
                     QHDYDKAVLVISELEKQI"
BASE COUNT         8353 a         3936 c         4210 g         7628 t
ORIGIN      
        1 gatttcattt gtttcggtgt cagtataact taggttgatt tcttcataag ttaatgtaac
       61 aatggttaga acatgttcca ctgaaactcc ttcactatct acataggtta aaacaatttt
      121 attgttttct acttcatatg aaccggttgt ttgaaaattt tgacaacttg aattaataaa
      181 ttcaaaatca ttttcttcaa aggttttatt ttcatttaaa accaagttat catcttcaca
      241 atcggtcgca ttaatataat cttcatatac gattgtatta tttgcgttga ttgtaccaat
      301 ttgatttaca taccattttc caagcacata attttcttgg acaaatgttg tatcatcatc
      361 ttcatcacat gaaatgaaca aggcgaagat tgacaaaaat ataagggata cttttttcat
      421 gttataggga ctaattattc tccgaaaact tcaatattat caacctgata agtagtggta
      481 acacctgccg cagaaccttc atatctgaag gctacgtaaa cagtaccatt aattccggac
      541 atatcaatgt ttcctgaact atagaaattg gatgcataag aagtatcctg gacatttaaa
      601 tcaacactaa attgagtcca agtagcggca ttaatggcag cagctgttcc ggaaccatcg
      661 taatctgtag aataccaaac tgaaagagca attccatttg caaatccggt tttgtatcca
      721 aaacgtaaaa attcagcggt tgtattatca agatttatag caggagtaat taaacgagta
      781 tctacattat tttcacctgt tccaaaagca gacatttgag catatttatt taaactaaaa
      841 attctagctt cccatttttc tgcacccgca tttatggata cattagacca acccggtaaa
      901 gcaataaatt gattatttcc tgtagaagtg ataccttcaa aactttcaga aaataatgga
      961 gccaatcttt ctccggttaa ttgaatatct ctttctgttc tggcaacaaa ttgataatct
     1021 gaattatatt tagtcattac acctctcact ttccctcttc cggttgcaac cggttttccg
     1081 gcaaagttag caaaactact ggttctgaaa ataacagaat ttccatcagc atccactaaa
     1141 aaatgattgg tggcacctcc tgtatcatta tttggatcgt aataagtagt agtaattgat
     1201 gcatcagtaa actgaacatc atcaatgtca attaatgtgt tgatgacagc atcgttttta
     1261 gcttgagcaa tagtcactgt ttttactaaa tcgctttcag gaacgatagt acatgaacgg
     1321 cttaagacag ttttatataa tgattcagaa agtcttccaa cagaaggtac attagaagaa
     1381 ttcacataaa ttccaccaat acgcattcca ccattactaa catcagtata taagtctttc
     1441 attttaatga atacttttct acccggttca aaattgataa aagtagaggt aacatcaaca
     1501 ggaacactaa atgcttttga accatctaag gtttgcatag agattgattt gtaaaaattt
     1561 ccgccttcat cacttgaagt aacataggct tcaatgatat catcagccaa atattgagaa
     1621 atatcagttg aagcaggtat ttgagctact tcttttgttt tttgtaaagt tgaatccaca
     1681 cattccaacg taggaactgc atagtcatca tcgttcacac agctcgtagt aaaacctgct
     1741 gcaagagcta taaataagat ggattttaaa aaattagttt tcatagtatt ttaattataa
     1801 gttgatgtaa aagttaacaa aataggttct tccgtaacca taaaaatatt ttggagcgaa
     1861 agagggcgtt ccactggaaa cgtcttgatt taattctctg taattagcat ttctggcttg
     1921 ttcaaaacca cctgttttat aagtaacgtc aaaaatgtta ttgattgaag caaagaaacc
     1981 aacagtattt ctgtttttat tgcttattct ccaagattta ccgccgacaa ggtttactaa
     2041 agtaaagcta tcaaattttt cttgttttag caattctcta gctctttctt ctgttgcttc
     2101 tggaaaaggg tagccattaa tatcagccgg atttctgtag aaattatctg ttctcattaa
     2161 agcagaaaca tctaaataat tatctgctaa atagtttaca tttgctccaa tccaccagaa
     2221 ttttgggtct ctatattcca tacctaagga ataagcttgt tgaggtgatc cagggatttt
     2281 ataatctttt aattttgatt cacctaaatt taccaaagga ggtaaattat ttaccgcatt
     2341 tgcatctaca tttaaataca cgttaggatt gtttgcataa gtatattgtc cgtatgcagc
     2401 agcaccattt actttaattg tggaagtgat ttggtattct aatccaaatt caccaccaaa
     2461 ttgtaattta tccatacctg taactaattc tgctacaaaa gcatctccgc catctccttc
     2521 agtatcttca aaagcacctt ctgcaaagaa gaaggaagtt tcagttatgt tttgaatttt
     2581 tgaataaaat cctgtgattt tagcttttaa tttaggtgtt ctgataatat aacttaaatc
     2641 agcactcgaa atcgtttcac tatctaatcc gtcaaaaaca aaattactta agcgagcatt
     2701 agggaaagta cttcttaagt taggtgcttt tgacatataa acaccattaa aattaataaa
     2761 atgtctacca gttagtttgt aggtcaatcc acctttaaat ccaaaattat caaaagcaat
     2821 tttttcacta tcgccatatg aatttgttgg ataaattccg tttttataaa gaccttctct
     2881 ttggtattca gaacgagaaa actcttgtgc tacatagaag tcaatttttt tgaaagtaaa
     2941 tttaaactga gtaaatgctc ttaatacgtt agcatataag ttatagttgt aaccgtattt
     3001 atcaccttcc actacttttc tatccggatt atttaaatcc gactgagaag catcaccatc
     3061 ttgaaaatta tcgatgtcgt caaaataaag accacctagt aaatcttcta aatattgata
     3121 gttatttgaa tttaatcttg tgtaagaagc tccaccatta agtccaatat tatcacttaa
     3181 ttgagagttt aatatcgtat taaatgtcca agttttgtca tctgttctgt cttgatacag
     3241 aacatactta cttcttgctt tttcacgacc gatttcctga ttattttcat caacaatagg
     3301 aatagaatta gctaaataca tggaatccca attaatctga cgttgattaa agaaattggc
     3361 atttgtagct ccaactaaat caggtgtaaa aataccaccc aatccacccg gttgatatgc
     3421 agcagggtct tgttgcatta cttcatccgg attattgttg tataatgatg taaagtaact
     3481 tggtaaattt ctgtaataag taggatcagg atttgttaca tcttgaaagt ctaatctgga
     3541 attagcgatg gaaccaaatt ggtatgttac gtttgaattt aatttagttt taggactaat
     3601 attccaataa tgactcaaca tgaaaatagg ttcttctaaa tctttatctc ttgaatttct
     3661 tttttcaccg tcttggtaac cccaataaga attatatttt tcgccggcaa tactagttac
     3721 ttcattagta ttcggagaat ttttccccct actgttttga gcataaatag aagtaaagtt
     3781 taaactatga ctattattga tttttttctc aatactggca aaaactgaat ttgcggaata
     3841 atctgttcct tcaaaataac cttcttgtgc gaaacgtctt gatgctgaaa taacaaaagc
     3901 ccaaccgtct ttgtccatac cagaggcata agtacccatc attctccaac tgtaattggt
     3961 attagttcct gaaaaagaca atctagttcc cggtctgtag atagatgctc tggtataaat
     4021 ttgttgagtc cctaaaattc caccaaaagt atggtcagaa gcagcagaac ccatagtgaa
     4081 gtcttggttt ctagtggcgt catttaaacc accccaattt ccccattgag gtctgttatc
     4141 ataaattttg ttcataatta ctccatttat catagtagta ctataatcgt tgtcaagacc
     4201 tcttattcta aatcttgctt gaccccaatt gaatgcactt gcttgttgaa agacatcacg
     4261 agttgcttgc agaagtccag cggtgttatc agaaccacta ttgtcatctc ccaagtcaga
     4321 ttcagtaatg gtgactagac ttaactgttg ctcagtcgta atgtcttctt caagagcaat
     4381 tactcctaag tctaatgttt gtccagctac aacatcaact gataataatt gttgtaggta
     4441 acctgtgctt ctcactaaaa ctaaatgttc tccaacaggt acattttcaa tactaaaaac
     4501 accttgtgca tcagtaagtt taattaatgt tgtgttttgg actgcaacaa caatactctg
     4561 aagcggtttt tgagttttag aatcaacgat tttacctgta atactcgatg tttgttgtcc
     4621 aaaaaccaca agcacctgca tcatgagtaa aatactaaaa actagttttt tcataaaaaa
     4681 tatagaatta atttacgttc gttaagttaa ttatttctta acagcccgca aatgtacatc
     4741 ttttaataat attatttact tttggtgcga agtttgtaat aaacttgata gtaatttaat
     4801 atacaattta tgaaaattaa acagttaatc gctgtttttg ttgtgatttt cgcaataaac
     4861 agtgtcaatg cccaagacaa aaagtataaa gtgcacacgg ttgcgtttta taatcttgag
     4921 aatctattcg atacaaaaga tgatcctaat gtttttgacg aagaatatac accagcacaa
     4981 ggatggacga gtgataaata ccaacaaaaa ttaaaaaatc ttgaaagagt tctttcagaa
     5041 attgcagtca acgaaaaaca agctaattct ccaacaatca ttggtgtttg tgaaattgaa
     5101 aacagaggtg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naacaagcta
     5221 attctccaac aatcattggt gtttgtgaaa ttgaaaacag aggtgtcctc gaagatttaa
     5281 ttaaaacacc taaattgtta acaaaagatt acggaattat tcattatgat tcacctgacg
     5341 ctagaggaat tgatgttggg tttttatatc aaaagaaaca ctttacgcca acaagttctg
     5401 caaacatccc attaatcata acaaattttg atggtagtgg aaaaagagtt tatacccgag
     5461 accaactttt agttacagga tatttagatg gtgaagaaat gcattttatt gtaaaccatt
     5521 ggccttcccg ttcaggaggt gaaaaaagaa gtagtccata tcgtgaagct gcaggagcct
     5581 tgaatagaaa gataatggat tctttattaa agataaatcc taatgcaaaa attattacaa
     5641 tgggcgattt gaatgatgga tcttataata aaagcgtaaa aaaagaaatt ggggccaaac
     5701 taaaaaaaac agaattaaaa gaaccaggcg atattttcaa tccttttgaa caaatggcaa
     5761 aagatggtaa tgcctctttg ttttatcgtg attccggtga tatttttgat caaattatga
     5821 tttcacagca atttgtattg gaagattttt caacttacca atattggaaa gcaggtattt
     5881 acaataaacc gtatatgatt cagcaaaccg gacagtataa gggatatccc ttgcgaaact
     5941 caccttctga accgggtttt agtgatcact tcccggttta tatatacttg gtgaaagaat
     6001 ataaataaat gtaaagccga tttaattcgg ctttttttat tttataactt tacaattcta
     6061 aaagtttatc taatggctat acaaaaacca ttcaatctaa ctaaatggat tgaaaaaaac
     6121 cgtcacttgc ttaaaccacc ggttggtaat aaaaatattt atgttgattc aggagattat
     6181 attgtgatga ttgttggcgg accgaacgcc cgaaaagatt atcattacaa cgaaacagaa
     6241 gaacttttct accaactcga aggaaaagcc aaagtgatta ttcaagaaaa tggtgaacga
     6301 aaggaattta aacttaatgc cggagatatg tatttacatc cggctaaaac accccattcc
     6361 cctatgcgaa gcaaaaactc cattggttta gttatcgaaa gaaaacgtgc cggaaaaggt
     6421 tttaccgatg gtcttttatg gttttgtgaa aattgtaatc acaaactcca tgaagtatat
     6481 tttgaacttt ttgacatcga aaatgatttt ctaccccatt ttaaacactt ttataattct
     6541 gaagaactcc gaacttgcaa aaagtgtggc actgtgatgg aaacagattc ccgttttgta
     6601 gccaagtaaa tcgctaaact tgaaacctga aactgactaa cggatagtgg ggctgcaatt
     6661 cagtactttg tatcaaaagc agttttcaaa aacaaaaaaa ataggtatct tcgcaaaaaa
     6721 atataacaaa aacctttaaa aaagttataa tgtcaaccat cgcacaacaa ttcggcatga
     6781 ccgaagcttt agcaatactt ggcgtaaaag ccattaacga aggaacttct accgggaatc
     6841 atcacttttc aaatggagaa atcctggaaa gttattcccc ggtggatggt caattaattg
     6901 gaaaagtaaa aaccaccaca gcggctgatt atgaaaaagt aatgcaaacg gcaactgcgg
     6961 cattcaaaac cttcagagca atgccagctc cacaacgtgg tgaaattgtt cgtcaattcg
     7021 gaaacaaatt aagagaatta aaagaacctc ttggcaaact agtatcctat gaaatgggga
     7081 aatcgcttca agaaggttac ggagaagtac aagaaatgat agacatttgt gactttgctg
     7141 tcggtttatc acgtcaatta aacggacaaa ccattccttc tgagcgacct ggtcacgtga
     7201 tgcgggaaca atggcatcct attggcgtgg tgggaataat ttctgctttt aattttcctg
     7261 tggctgtttg gtcttggaat actgccttgg cttggatttg tggcgacgtg tgtgtgtgga
     7321 aagcatccga aaaagctcca ttatgttcta tcgcgtgtca aaatattatt gcaggagttt
     7381 taaaagataa taatttaccg gaaggaattt cttgtatcat caatggcgat tacaaagttg
     7441 gtgaaatgat gactaccgac actagaattc ctttaatttc tgctacagga tccaccagaa
     7501 tgggaagaat tgttggaact acggttgctc aacgttttgg aaaatcatta ttagaattag
     7561 gaggaaataa cgctattatc atcacaccaa ctgcagattt aaaagttgtg gttccgggtg
     7621 ctgtttttgg tgctgtggga actgccggac agcgatgcac ttcaacaaga aggttaatta
     7681 ttcatgaatc agtttatgac aaagtgagag acgcgattgt tggagcctac gaacaattaa
     7741 caattggtaa cccactcgat caaaaaaatc atataggacc tttaattgac accgatgcgg
     7801 taaataccta tttagcagcc attgaaaaag cgaaagcgga aggtgggaaa gtactagttg
     7861 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacaatcat tttgaaattg
     7981 tacaacacga aacgtttgct ccaattttat atcttatgaa atacagtggt gaagttgaaa
     8041 atgcaattga attacaaaac ggagttgcac aaggattgtc ttctgccatt atgacaaatg
     8101 aattgaaaga agctgaaaaa ttcttgtcat tttccggctc agattgtgga attgccaacg
     8161 taaatattgg aacttcagga gcagaaattg gcggtgcttt tggcggtgaa aaagaaacag
     8221 gtggcggacg cgaaagcggc tctgattctt ggaaagtgta catgcgaaga caaacgaaca
     8281 caatcaatta ttctgaccaa ttacctttgg ctcaaggaat taaatttgat ttgtagttta
     8341 atagagataa tagaagaaag aaaaccctgc aaaaaaattt atttgtaggg ttttttattt
     8401 tcttgttgtt ccgaatcaaa gaagggatct aaaataataa tgaacagttt tcgccctttt
     8461 tacgcaacct attttttgaa tttgcatctg attagaaacc aattaataaa tacatgaaaa
     8521 aaataatttt agtactaact tgtgtgccgt ttttgttggc aacaacttgt gaggacgatg
     8581 atgatcaaat ttattgtaca caagaagcaa aagcagcatt gaatgtttcg gttagtttag
     8641 gagcaatgag ttccatcacc agtgaaggtg taactgtggt tgcaacagat ggtaattatt
     8701 cagaaacact agtagtggtg aatgaaaatg atccaatttt ctcaggagct tatgaaagag
     8761 aaggcagtta tactattaca gtttcaaaag aaggatatca aacgcagaca tctgaaatta
     8821 ttactgttac tagagatgtg tgccatgtaa ttccgcaatt tattcatgtg gtgttagaac
     8881 ctaattaaag tcataatttg aaataaattt aaaaaccctg caaagtttga gttttgcagg
     8941 gtttttgctt taaaaaattg ctttaatcaa tctaaattta aacaataaaa taagtaataa
     9001 taggcgacaa aaggtagagg ataatattat aaatatttgt aactttgata agtatctgta
     9061 tttagattta aatcatataa aaggtttgta atctagttaa aaacagattt atattttttc
     9121 atttctttct ttgaaaaatt ttaatcttat gaaaagaaaa ctaattagta caactgcact
     9181 tctgctacta gtacagtttg ccatttgtca aacatcttcc aacctcccgg aaaaaaagag
     9241 acccaatatt gttgtcatat tagcagatga catgggttat tccgatatcg gtatgtttgg
     9301 cagcgaaatt aaaactccca acttagatgc attagcttca aatggagtac gttttaccaa
     9361 tttctataca catgccagtt gttcgccctc gcgatctatg ttgttatccg gtgtagacac
     9421 acacctcaac ggactaggaa atatggacga atggaccgca ccaaaccaag ttggtgttga
     9481 aggttacgaa ggaaatttaa ataataaggt tgtcacggtt cctcaattat taaaagatgc
     9541 cggttatcac acctatatgg ttgggaaatg gcacttggga aaatcacccg acaaaatacc
     9601 tgccgcacga ggctttgagc gtgattttgc cttgctggac ggaatgggaa gctattggga
     9661 catgaccaat atgaccgctt tggttcctaa ttcgcttttt acagaagatg gtaaatacct
     9721 caccaaacta ccgaaaaatt attatgcaac taaaacatat accgaaaaaa taatcagctt
     9781 cattgatgca gatcacaaag acggcaagcc tttctttgct tatgttgctc accaagcacc
     9841 acatgacccg tatcatctac ccaaagattg gaccaacagg catgtgggac aatatgatat
     9901 tggttgggat aacatacgac aacaacgtct caaaagacaa aaagaactcg gtatagtccc
     9961 agagtcggct agccttgctg aacggatgtg gtttattcct gattattcga ttttggctcc
    10021 agctacacga gctgtattgg gtagaaaaat ggaactttat gcaggtttag tagaaaatct
    10081 tgattttcat attggtcagc ttgttgacca tttgaaaaaa attggggaat acgaaaatac
    10141 tatctttatc attttaggag acaatggagc tgaaggcaac gaccttggtg aaatgattgc
    10201 cggtcaaaaa ggatcgctca attcgttgtt ttttgcttcc aagtggtcca ataataatat
    10261 caacgcatgg ggagagccag gttcgtatgt aggttatggt cccggatggg ctcaagcttc
    10321 aatgacacct tttggtcaat acaaaggttg gcttggtgaa ggcggcattc gaaatgctct
    10381 gattgtaagt ggtccggtga atagaagaga aaaagggagc atcaataacg gtttaatgtc
    10441 aattgcagac atcatgccaa cactcttgga tgttgcgggg acaacctatc ctaaaaatta
    10501 taacggaatc caattacctc cgatgattgg aaaatcatgg acaaatgttt tagccggtgt
    10561 tgctgattct cctcgtacaa atagtgatta tattgcttgg gaactttttg gaaatcgtgc
    10621 gttacgtcaa ggcgattgga aattgcgatg ggaatatgct ccttttggta aaggcgattg
    10681 ggaattattt aatgtggctc aagacccatc cgaacgaaac gaccttgccg caaacaatcc
    10741 tattaaatta aaagaaatgc ttttgctttg ggaagaatat gtagcaaaaa ataatgtgat
    10801 tctaccaagt cgctctccat ttgaaggagt ttatgatgtg cttcctgcca gattcccaat
    10861 tgaagaaggt tttcctcctc ttatttataa acgtcaatat actccaccaa aagaattaat
    10921 gaacacttca aagcaatagt atcatgaaaa ataatttaac tgctttaagc ttcaatttca
    10981 ctaagaaatt atacctactt acaatctttc tattattagg ttgcacaata gtaatacaag
    11041 cacaaggaga tggcccgaga ttttattgga aaactttaat gggaacgaat gcagttcctg
    11101 taattacttc atcaatgagc ggaaattcaa acccatttga tccttctcgt tttgttattc
    11161 ctggctctaa ctttgaagct acaatgacca tggcaggata tgcaaaaatg ttgccacttt
    11221 ttaagcgatc cgcaatggtt tcagtagtta tgccaatggg aagattaact agtgatgttt
    11281 caattaatgg tttagacttt agcaatacgg ctagaggttt tggtgatcct atgttaatgt
    11341 taagtgtaaa tttaatagga gcaaagcctc agatgaatat ccctgatgca cttcgttatc
    11401 aacctaaatt ttctttggat gttctagcct cttttgccgt tcctattgga gaatatgata
    11461 gttcagttcc tattaattta ggtcaaaata gatggtacgg aagaattgga acgcctatag
    11521 tttggcaaat tggtccatgg gttccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnntatcg cgaaggtagt tttgtatgtc aaatggatta ttcgtgggga aaacccaaac
    11701 gtattatttt tatgcgaatg ttaaacgaag aagaagccga aagaattaaa aataaaatga
    11761 ttcggggagg ctatttaaca ggagtcagtt tagatgataa aggacagacc tattcggaaa
    11821 acacagcttt tacagaattc cattatcgta ttccagtaaa aggttctttt ttaattacac
    11881 acagactacg tgccgattta cgatggatgg gaaatgaaaa tgaattttct caaaggtatc
    11941 gctatcgatt tatgattgag aaagaattaa aagccaaaaa agtttcattc gtaccttata
    12001 taaatgcaga aactttttat gattctcgtt ttaatacaat taatcggttt cgaggtattg
    12061 caggaacctc tgtttcatgg tccaaacatt ttgcaattga agggaatttt acttatcaat
    12121 atgattcaaa atcctctgta acaaatctct atgcaactaa tttaatcttg catatttatt
    12181 tcgaaacgaa agcagcttta aattaattat ttagtctttt tcacgtattg agtcaaaata
    12241 taaatagttt gattttcaac ccgtccttca attaaattag catcatccca aactaatttg
    12301 atgttgtgaa cagcagctcc gcgtttcgct gtaaaattag caccttttac atctaaatct
    12361 ttaatcaaaa caactgaatc gccgtctttc aatatgtttc cattcgaatc tttgtgaata
    12421 atttttcctt cttcctcatc tccttcattg gttgattttg cccacgctaa agcatcttca
    12481 tccaaataca tcatttccag taaatcatga ttttttaatc gggctaacat tcgccaagaa
    12541 acgatttgta caggtagatt ttcgttccac atgctgtcat ttaatcctcg ccaatctgct
    12601 gcatttgttg tttccggatt ttctatttgg tcttttaacg cttttgtaat caaaatactg
    12661 ttttctaaac tttcctctgt tttaggggct acagtataaa ctactaaatc gtcttcaatt
    12721 ccacttattt cacattttga atgacttcgg tcttgtaatc ttttttcgat gatgctcatt
    12781 tggaaatttg attatagatt gaaagaagca ccaacgctaa tttgaaattg gttattcaat
    12841 tgttcatagc ctacagggtc actattgtat ttagcaattg gaaatccaac ttctgaaaac
    12901 acaccaaatc catccgaaaa gaaatagcga ataccggcat gtgctccaaa atttctaagg
    12961 ccaatattta aaccgggata aatatcaatt gcttcaggta atttcatgac acttccaata
    13021 ttagcactaa aacgagcttt gatatcaaat tcatcttcaa atttagaagc atctccatca
    13081 acacctaaaa gataaacagt ttgcaaaccg attgacatat tttctccaat tccacgatca
    13141 taaaacaaag taattcctga tgcatgatct tgaaagttag caccaacttg aaatttttga
    13201 tctccttttc cgctataagc ttgtgcatta gctaaaaatg cacttaaaat caatccaata
    13261 gtgaataatt ttttcatatg tagataatta tttttttata agtcatatgg aatcgcttta
    13321 ttttttatgg gtgtttgctt acgcaaacgt gttgttaata gcgatttcat tgttataaaa
    13381 tttttgcaaa tgtaatcatt tcaattttaa cccgaaaaca ctaatttctt cctttttcat
    13441 tgggatataa ttgtggcaag ggttcacttt gccaatattc tttagttttc acatttaaaa
    13501 ttgttaatgg gccaacaaat gccgcaccgg tatcaatgtt ccaaatacag gctttgtttg
    13561 ttggaattgt ttcgtttatt cgtgttgttg gtgtatggcc aatatagatt tcgtcatata
    13621 atttgagacg ttttggataa aaaatactgt ctttagaaag ggtaggattt aaacttagtg
    13681 ctgtttccca caaggttcta tcccaataaa ataatttcgg aaaatattca aaatcaattc
    13741 cgttcatatt ggtaaaaccg gcgtgaataa agagtctgtt ttggtcatca agataataat
    13801 cttttaatcc ttctaaaaat tcgatgtgtc tttttcgttc aacagcagaa accttttgat
    13861 aacaatcgat ggttgattga cctccgtgtt taaaccactc ttgatgatag gaatttgttt
    13921 taagataatt caacaataaa tcatcatgat tgcctcgtat gaaaatacaa gtttgttttt
    13981 tatctaattc aattaaaaaa tcaagtactt gtggtgattc gctccaacca tcgacaaaat
    14041 cgcctaagaa aattaaagtg tcttctcttg tgacttggct tctttccaac acctgatgta
    14101 aagcccgaag accaccgtga atgtcaccaa ttacgaatgt attagtcatt gtttttatca
    14161 tatttcattt ttctcaaaat gcgtaatgtt tctttaaaat ttacataaac cgatgggttt
    14221 tctaatgctt ttagtaaagg cttggcatca attaattttt gtttagcgtc ttcaaatcca
    14281 ttcaaaaaac aaatcatcag cgtagaaggt aaattcaaca aatctctttt ttctctttca
    14341 gataaggaaa ttcctttttg taatttctgc aacaaagata agtttgaatt gtaaatatga
    14401 aacatcattt ttcgattgta ttgaggcggt tcaaataaaa agaaagtttc aattttgtaa
    14461 tagccgttgt caaaaaaggt cagcgtttgc ttttctagcg gataataact cgttttgtca
    14521 tttaatccta aaggaacata ttcagtaatc gaaaaagttt tatcatcata ttctatcgtc
    14581 acttcatcaa aggcggaata aaaaagtttt acttgctcat taattaattc aatatccact
    14641 ctggaaaaaa aggttttatc acgtactttt ttcttatgac cggcccaaca aacgacaaaa
    14701 agttctttga aaacatggta gttggtagcc atatctttat gtcggttatt taaaaaatct
    14761 aaaacgcctt ccagggtgtg tgaaatactg tttctcgaat tattttcaat gccaattact
    14821 gtttcagtgt tcaattttga aaagggaact ttatcttctg tggggataga attacttcct
    14881 ctacgaataa aaacggtacg agccggagtg gtgtgaattc cttttttaaa aaaagaaacc
    14941 ttagaatttg cttttatggt taccaatcct actactttat cttttgggag atttggaaaa
    15001 ggaacatttt catattgaat tttcggagga ttttctaaat aagcattcac taaattttga
    15061 attcgactat catcaaaaaa atcatctccg gtaatctcat tatcctgatc ttctacaccc
    15121 acaacgatat acgaattgtt ggttgggtta gaattagata atgcacaaat atgttttaaa
    15181 aattttgcct ttccttcttt agaatgcaaa tttaactgac gctttttatc atagaaacta
    15241 ctctcatcgt tatgagccaa aaggtttttg ataaggaggc gtttgttgat cattttttta
    15301 gtctacagtc gcagtcacag ttttcagtgt attgatagct tgaaaactat ttctgcgact
    15361 gaaaactgcg actgaaaact ttattaaggc actttatttc caatgctttc tgtccaaatt
    15421 tcaaaccaat cttctttgtc taaaattagt cctgctgctt tttgtaattg ttgaattcgg
    15481 gctacattaa cagttcctgc aacaggaatt acttttgaag gatgttgcat aatccaagct
    15541 aacaaaagca aatcggaacc cacttgatat ttttctatca atcgggcaaa tagttttttt
    15601 aatcttcggg tttggtcagt gtcttctcta aaaacggttc ctaacggatt ccatgccatc
    15661 ggacgtattt ggtgaaactg catgtggtct aaagttccat ccagcattgg ttgaaaatcg
    15721 gttgccgaaa actgaatttg attataagaa acttttgttt tttgctgtat taaatctgtt
    15781 tgtgaaggcg taaaattcga caatccaaaa tctaaaattt taccgtcttt ttttaattgt
    15841 tccaccgctt cagcaatttc atcggctacc atcagtggac tgggtctgtg taataaaaaa
    15901 acatctaaat aatcggtctg taaattttta agggaatttt ctactgacca aaaaatatat
    15961 tccttcgaat aatcatagtg tttgattctg ttgtttcttg tatcacaaac atgttgaatg
    16021 ccacattttg aaattaattg aattttagaa cgatctaatt tacttttttt taatccaatt
    16081 ccaaaagtgg attctgtggt atatcctcca taaatatcag catggtcaaa tgttgtaatt
    16141 ttgttttcta aacaaacatg aatcaaattt gccatttcat ctgagcttaa atttttgtcc
    16201 caaacacccc aattcatggt tcctgcaata acagtagata agttagtttt catagtattg
    16261 tattgataaa tttaataata ttgacttttt tcaagaaatt cttctgtttt tcttcattcc
    16321 tttttcatcc atttccttca ggtcttaaaa ttataaaaac taaatcacaa atcactctta
    16381 aaacccatgt tttaaaagaa cttttaacgc tatattaaca tcttgaaagg aataaaattt
    16441 tcaatttgca ctgttaaaaa attgatagta atttcgtgcc tttaaaaaat gaattatgga
    16501 agaaaacact acgactctag acattagagc gattaatgag aaaattgaaa gagaaagtgc
    16561 gtttatcgac ttgcttacct atgaaatgaa caaagtcatt gtcggacaaa agcacatgat
    16621 tgaacgattg ttgataggtc ttttaggtca aggtcacatc ttgttagaag gtgtgccggg
    16681 attagcaaaa acacttgcca ttaatacatt atcacaagct gtacaaggtt cattcagcag
    16741 aattcaattt acgccggatt tattacctgc cgatgtggta ggaaccatga tttataacat
    16801 taagcaaaat gaattttcca ttaaaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16921 nnnnnnnatt gattatccaa aaatggaaga cgaacgaatg gttattcgtc aaaatttaaa
    16981 aggaagtcat gaaaaagtaa acgccgtcat ttccattgaa caaatcttga gagcacaaga
    17041 agcagttcgt gaagtttaca tggatgaaaa aattgaaaaa tatattttag atatcatctt
    17101 cgctacacgt ttccctgaaa aatataaatt agaaagtcta aaaccattaa tcagtttcgg
    17161 ttcatctccc cgaggaagta tcaatttagc tactgcagca aaatgttatg catttatcaa
    17221 acgtcgtggt tatgtaattc cggaagacgt tcgtgcagtg gttcacgatg tgttgcgtca
    17281 tagaataggc atcacgtatg aggcagaagc tgaaaatatt acttcagttg aaattatcaa
    17341 caaaatagtt aatgagattg aagtacctta atataagttg cgagttttaa aagttgcgag
    17401 ttttaaagtt atttaaaaac ccgtaaccta taacccgtaa cccgtaacta aattatggaa
    17461 acaaaagacc tactcaaaaa agtacgaaaa atagaaatta aaacccgaag attgagtgat
    17521 cacatctttt caggtgaata ccacacgtcg tttaaaggcc gtgggatgac tttttcagaa
    17581 gtgcgtcagt atcaatttgg tgatgatgtt cgagccattg attggaatgt aacggctcgc
    17641 tacaatgagc cctatatcaa agtttttgaa gaagaacgtg agttgaccat gatgttaatg
    17701 gtagatattt ccggttctga aagttttgga acaaaaaatc agttgaaaag tgaaattgta
    17761 acggagatag cagcaacaat ggctttttca gctactcaaa acaacgacaa aattggtttg
    17821 attttatttt ccgatgaaat tgagctttat attcctccca aaaaagggaa atcgcatgtg
    17881 ttgcgaatta ttcgtgaatt aatcgaattt caacccaaaa gcaataaaac caatatttca
    17941 aatgcattga aatttttatc cagtgtgatg aaaaagaaag ctattgtttt ttttatttct
    18001 gattttatta ccgaggataa ttatgaacat acccttaaaa ttgcagggaa aaagcatgat
    18061 ataaccggaa ttcgagtgtt tgatattcgt gaagaaaaaa tgccaaatat cggaatggta
    18121 aatatgctcg atgctgaaac aggcgaaaca atgttggtaa acaccaattc aaaatcgatt
    18181 aggatggagt atgaaaaata ttaccacgaa aaagtaattt ttttcaaaga aactttttca
    18241 aaatgtggtt caggaactgt aaacacaaga gtagatgaaa gttatgtaac taaattatta
    18301 agctacttta aatccagata atcattagca aatttcaaac taacctttgt gaatagctgt
    18361 gaaacaaaac atgaaaataa aattatatat attctttatt ctgttctcaa ccttagtttt
    18421 tgctcaacaa aaaagagtga caacttcggt agatagtact aaagttaaaa taggagctca
    18481 aattaatttg actttaaaaa ctacggttga tacactttca aaagtgactt ttccggaagg
    18541 taaattattt ggacgtttag aagtacttga aagttatcct accgatacaa tcaaggaaaa
    18601 ttttaagtat caactaatca agaaatatgg tttaactcaa tttgatagcg gaaaatttgt
    18661 cattccttct ctacaggtta taatcaattc aaaaccttat ccaactgata gtgttttggt
    18721 tgaaatttca aatgtggtag ttgatacgtt aaaacaacag atgtatgata ttaaagatat
    18781 tacccaagtt gataatgacg gaaggccttg gtgggtatac ctattaattt tagttgtatt
    18841 agcaggaatt ggttatttga tttattattt aattcaaaaa aacaaaaaac ctaaagttga
    18901 agaaattgtt tatgcttcgc cgatagaaaa agcaacagct cacctaaaaa cattagaaaa
    18961 aaagagctta gtagaaagag gagaagtgaa agcttattat tctgagttga cagatattgt
    19021 ccgaacatat attgaggaag ccattgaaat tccggcaatg gaaagcacaa ccgacgaatt
    19081 gattgcagct ttcaaaattg cgattgtaaa aaagaaatta gcattaaatg atgaaacgat
    19141 aaaaaacctc gaaagggttt taaaacaagc agatttagta aaatttgcca aatcaaaacc
    19201 acttgaattt gaaattgtag aagacaaatc aaaaattgaa aaaacaattt ataaaattca
    19261 ccaatcaatt cctgaagttg tagaagagga gttaatagac aatgatttta acaaagataa
    19321 aatcctaaaa agaaaacgaa aacgcaaaat cacaaccctt gttttatcat tcatcgtttt
    19381 actttttgta gtgacgggaa ttttaattgt aaccaaagga tttgaatttg taaaagacac
    19441 aatcattggt catccaacca aagagttatt ggaaggggaa tgggttttga gcgaatacgg
    19501 taatccaccc ataaaaattg aaactccaaa agtgttgaaa cgaatggatg tttctaaata
    19561 catgaacaaa gaatcggctt ccttgatgaa agacatgcaa atgtttggat atggagctat
    19621 tttagatcat tattacattg ttataatgac taataaattt aaagaagaaa ttaagattga
    19681 cttagcaaaa gcaatggaag ggtcgctcaa aataatggaa gcacaaggag cacagaatat
    19741 tttattaaaa caagaagaat tcagttctaa agaaggtttt aatggattaa gaggctacgg
    19801 tacaatgaca attattgatc cgataactaa gaaaagcaga aaagcctatt atgaaatgat
    19861 ttacttttct caagagggag gtttgcaaca agtcatggtg ttacaagatg aaggagatga
    19921 atcagctaat caaatcacag aacgaattat gaattctgtt gaaattgcca aaatgaaata
    19981 actatggaaa acatcacctt tttacatcca cagttttttt ggttattcac tttattgccg
    20041 gttgcaatag cttggtatat ttggaagcga aataaacaaa cggcaacatt aaaagtgagt
    20101 tctttgaaag gatttaaagt aaaaagttca gtcttgccta aactaaaacc aattcttttt
    20161 gtgttaagaa ttttagtttt aagtttttta attgttgctt tagcaaggcc aagaaccgtt
    20221 gacatcagta ataaaacaaa aaccactcga ggaattgata ttgttatggc aaccgacgtt
    20281 tccgggagta tgttagccaa agatttaaaa ccaaatcgat tggaatcgtt aaaaaaagtg
    20341 gctgctaatt ttgtaaatga ccgtccaaat gatcggtttg gtatagtggt ttatgccgct
    20401 gaaagttata caaaatcgcc tgtaaccagt gataaatcgg ttgtgattaa taccatcaaa
    20461 agtattcaat atgataatat tattaaagat ggaaccggaa taggaatggg tttggccact
    20521 gccgttaatc gattaaaaga cagtaaagca aaaagcagag tcatcatatt gttgacagat
    20581 ggggtgaaca atgccggatt tatagagcct gaaacggcag ctgatattgc aagagaatat
    20641 ggaataaaag tttatacaat aggtttagga acaaatggca tggctgagtt tccttatgcc
    20701 tatgcaccta acggaggttt tttattccga atgatgcctg tagaaattga tgaagcttta
    20761 atgaaaagca tcgcaaaaaa aacagatgga aaatatttca gagccactag caatagcaaa
    20821 ctcaaagaaa tttatgaaga aattaataaa ttagaaacaa cagaaataga agaacaaaag
    20881 ttttataatt tcgacgaaaa gtttcgtcct tttgtttgga ttgccgggtt attattattg
    20941 gtagaaattt tgttaagaca aacgattttt agaggtttta tataacatgt acgaactgga
    21001 cgaaaaaaaa tatttatact ttttagccat tatcccaata attgtgctgc tttttctatt
    21061 caatttatat tggaaaagaa aaaagcaacg tgaatttggg gatattgaat tggtaaaaaa
    21121 attaagccct gataaatcaa tttttaaacc gattttgaaa attgttgttt tgttattcgc
    21181 ttttgtttgc atcgtcatag gattagtaaa tccaaaaatt ggcactaaat tagaaactgt
    21241 caaacgagag ggaattgatg tggtatttgc catcgacgtt tcaaaaagta tgttggcaga
    21301 agatgttgct cctaaccgat tagataaatc caaacaaatt gtttctcaaa tcattaatca
    21361 gttgggaagt gatagaatcg gaattgtagc ttatgcaggt agtgctttcc cagttttgcc
    21421 cataacaacc gattacggtg tggccaaaat gtatttgcaa agcatgaata ctgatatggt
    21481 ttcctcacaa ggaacttcat tggaagatgc aattaggttg tcggctaact attttgacaa
    21541 aggcaattcg acaaataaat tgattgtttt aatttctgat ggtgaagatc attcagaagg
    21601 ttttgaagga gccattgaag aagccaaaaa aataaaagca aaaattatta ccatcggtgt
    21661 tggaacagaa aaaggtggcc caattccact caaaagaaat ggtgttgtgg aaagttttaa
    21721 acgagaccaa aatgatgaga tggttgttac aaaattaaac accgaagttc taaaacaaat
    21781 tggaaaagga accaaagggg gttatgtata tgggagcaac accaaagaag tgctagaata
    21841 cgttaaaaaa gccttggata atattgaaaa aacagaattt gaaacgacac aaattgctga
    21901 ttttcaatca cagtttcaat ggtttttagg ttttgctttt ttattattaa tgatagatgt
    21961 ttttctattg gaacgaaaaa ctggatggat aaacaagttg aatttattta atgaaaagaa
    22021 agcatgaaac aaaatttaac cattttactt ttaatgctat cctattttct acaagctcag
    22081 gaagagaaaa aagaaaaaga taaagtatta ccaaaagcta ataaagaatt tgtggctaaa
    22141 aattttacag aagcagaagc acaataccgt gtttcaaatt ctaaaggagt ttccggagcc
    22201 acaccagcct ataatttagg aaattctatt tataaacaaa atcagccaag cgaagcaaaa
    22261 tatgcttatg cgaaagctat agaaaaagcc aaaactaaac cggaaaaaca caaagctttt
    22321 cacaatttag gcaatgtgtt tatgaaagaa aagcaatatg caaaagccgt tgaaagctat
    22381 aaaaatgcct tacggaataa tccaaccgat gaagaaaccc gatataatta tgccttggca
    22441 aaactgtatt taaaaaacaa tccggaagat cctgaaaaag acaaaaaaga cgacaaaaaa
    22501 gacgacaaaa atcaggataa aaaagatgac ggagataaag ataaaaagga agataaaaaa
    22561 gacggcgaca aagacaaaaa tcaggataaa aatgacagga acaaagacca agatcaaaaa
    22621 agtcagccaa agccacaacc atccgggatt tcaaaagaaa gtgtccaaaa tttgttagat
    22681 gcagtcaata atgaagaaaa gaaggtgcag gaaaaagtga atgctcaaaa agtaaaaacg
    22741 aaacctgttc aaacggaaaa agattggtaa aaaaaatgaa aaagtttcta ttcatagtat
    22801 ttttaagctg tcaggcaatt tttgcccaag ttcagtttga ggccaaagtg agtaaaaact
    22861 cgttgggttt aaatgaacga ttaagggtag atttcaccat gaatgtagat ggtgataatt
    22921 ttgtaccgcc aacatttgat ggttttagag tgattgccgg gccaagtcag caagtgagtc
    22981 aatcatgggt aaatggtaaa agttcattca ataaaactta ttcctatttt ttattgccta
    23041 cacaaaaggg tactatttca attaaacaag ctgcaatcga gcacaatgga caagtttata
    23101 aaacaacacc tatttcggta aaagtttcgg atgccgttga acaacccaga gatccgaacg
    23161 acaattcagt ttccataaac gatgccttgt atttagtggc cgatgtcagt aaaacaaatc
    23221 catatttaaa cgaacctatt acggtcgttt ataagctgta tttcagtcac aatataggaa
    23281 tcacaaattg gcaggaatta gacaagccta aatacaataa tttttggagt caaaacattg
    23341 atattaagca attagtagca gaagaaggaa cttttaaagg ggaacgtttt cgttacgtgg
    23401 tactcagaaa aacagttttg tatcctcaaa aaacgggtag tcttgaaatt gaacccttgt
    23461 cattggatat cgatgttgaa ttgcctacta accgaagaaa tatttttggt caagtaatgt
    23521 tgaccaaaga ccataaacgt gtttccgcag gaagtaaaat tatttctgta aaaccgcttc
    23581 ccgaaaatgg caaaccaatt gattattcag gagcagtcgg taaatttgat tttaaagtta
    23641 tcccatcccg aaccaattta aaaaatggtg aaagtatgga cttagaagtg agtatttccg
    23701 gaaacggaaa cctcaaattg tttagtttac ctaaaccggt tgtgcccaca gccttagaaa
    23761 tgtatgatcc ggtacacaca gaaaatgtga atactccttt gtcagggatg agtggaaaaa
    23821 ttacagataa atataccata attccacaat ttaaagggag ttacacaatt aaaccgttat
    23881 ccttttctta ttttgattta agtacgggga agtataaaac gattacttca gaggaaatta
    23941 taatcaacgt gacagatggg ccatctcaaa atggtaatct gacagcaaaa acaacagaat
    24001 ctgttgataa aaaagctatt tcggccgggg atcaatttaa attcattcac ttaaaaacga
    24061 aattttcttc cataaataag caagatttct ttggttcaaa cctctattat gttttagtaa
    24121 gtattccctt tttattgatt ccgtttattg ttttattcaa aaagaaaaaa gaagctattg
    24181 atggagatgt tattgggaat aaattaaaat taaataacag gttagcgaag aaatatttgt
    24241 cagaggctaa aaaacaatta gggaataaag aaccatttta tgtagcatta gaaaaatcat
    24301 tgcacaattt cttaaaagct aaacttaaaa ttgaaacttc ggagatgagt aaagatatta
    24361 tcaaagaaac tttagcaaat aaaaaagcac aaacggaaac tattgagcat tttattcaac
    24421 tgactgaaaa ttgtgaattg gcaaggtatg caccatcatc aacagttgct atgcaacacg
    24481 attatgacaa agcggtttta gtaatatcag aactggaaaa acaaatt
//
LOCUS       DAOTCW010000018        23739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1105_length_23739_cov_43.746580, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000018 DAOTCW010000000
VERSION     DAOTCW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 23739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23739
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1105_length_23739_cov_43.746580"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="P5335_02655"
     CDS             <1..1084
                     /locus_tag="P5335_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRZ73814.1"
                     /translation="THVQTITVQDTTAPTFVETLPQDTSAECSNIPVAATLTATDSCG
                     DATVTYTEAIVQGECTGDFVITRTWTATDLCGNSVSHEQIIIVQDTIGPVSSPFDENI
                     SVTCEAIPVSPDLTFTDACSTVGGVTFNEETVNESATSYTIVRTWEAYDSCGNLSTFT
                     QTINVTIQDSFTPISSTSCIGDSTPIDLNTLLPTGTTGGTWIDVNNTGALNNNIFTPT
                     LVAAVGNYIFRYEITGGSCPRVYEVTMNVNADCIVFPCDAINIYNAMSPNGDGLNEFF
                     NIENIENTECYPSNSVEIYNRWGVLVYDVQNYDNSSKAFRGVSEGRATVNKSAELPTG
                     TYFYIIQYTTFDGDVVNKSGYLYLTR"
     gene            1137..2048
                     /locus_tag="P5335_02660"
     CDS             1137..2048
                     /locus_tag="P5335_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRZ73815.1"
                     /translation="MRTKFLFFALMLTGFAGFAQQDAQYTQYMYNTINVNPAYAGSRG
                     VMSIFGLHRTQWVGLDGAPVTNTASINTPINESNLGLGLSFVNDRIGPTNENTISADL
                     SYSINTSETYKLSFGIKASANLFNLDVNKLNPADQGDPQFADLENKFTPNIGAGVYFH
                     SDKTYFGLSVPSFFETTRYDDNSVAINKERMSFYFIAGHVFDLSYNLKFKPALMTKAT
                     EGAPLQVDLSANFLIMDKFVLGAAWRWDAAVSAMAGFQVSDGLYIGYGYDMETTELKN
                     YNSGSHEVFLRFELFQRHNKIVSPRFF"
     gene            2061..4001
                     /locus_tag="P5335_02665"
     CDS             2061..4001
                     /locus_tag="P5335_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRZ73816.1"
                     /translation="MKIKFIYITLLSCIALNVSAQKAGVKKADKNYDKFAYIDAIKTY
                     ERVAEKGFKTPDMLQKIGNAYYYNADLVNAEKWYGELFAMTEEVEPEYFFRYAQSLKG
                     VGNYAKADEMMAKFNLKSGNDSRGLIAKTKKDYREEIKKNSGRYKIEDAGINSEYSDY
                     GPAFYLDKVVFTSTRDTGNFSKRKHSWTDQYFSNLYSADMTTEGVLSSAGKYGKKLNT
                     KFHESTPAYTKDGKTVYFTRNNFLDGKKGKDANKVTLLKIYKATLEEDKWINITALPF
                     NSDSYQVAHPTLSADEKTLYFASDMPGTKGLSDLYKVDIKEDGSFGTPVNLGSDINTE
                     GRETFPFITSDNELYFASDGHPGLGGLDIFITKLEKDGSFKQVHNVGESANSQYDDFA
                     LIINKTSKRGFLTSNRPGVGNDDIYKFLETREIDWDCEQSLVGIVTDANTGAILPNTK
                     LTLFDEKFTKLRDTISGLDGRYDFGIVDCEKKYYVRAEKEAYTTKEVPVIIAKESGIT
                     ELPIELDSAVKAIPVGGDLADVFGINLIYFDLDKWNIRPDAAIDIAKILDVLNQYPTM
                     KIDIRSHTDSRQTHKYNEVLSDRRAKSTRDWLIKHGIAPERLTAKGYGETQLVNKCAD
                     NVECSEAEHQLNRRSQFIITDL"
     gene            complement(4086..5720)
                     /locus_tag="P5335_02670"
     CDS             complement(4086..5720)
                     /locus_tag="P5335_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRZ73817.1"
                     /translation="MITVNDIAVHFGGTTLFSDVSFAINENDKIALMGKNGAGKSTLL
                     KIIAGEAKASTGNISAPKDAVIAYLPQHLLTKDDATVMEEASKAFAEIFDMKNEIDHL
                     NEQLTIRTDYESDDYMKLIERVSEVSEKYYSIEEVNYEAEVEKVLKGLGFEREDFNRP
                     TSEFSGGWRMRIELAKILLKKPDLILLDEPTNHMDIESIQWLEEFLINSAKAVMVISH
                     DRAFVDNITNRTIEVTMGRIYDYKAKYSHYLELRKDRRVHQQKAYDEQQKMIADNMEF
                     IERFKGTYSKTLQVQSRVKMLEKLVPVEIDEIDNSALKLKFPSSLRSGQYPVVVKDLE
                     KSYGDHLIFKDANIVIERGDKVAFVGKNGEGKSTMIKAIMKEIEINGGSVEIGHNAQI
                     GYFAQNQASMLDENATIFETIDDIAVGEVRTQIKNILGAFMFQGDDITKKVKVLSGGE
                     KTRLAMIKLLLEPVNLLILDEPSNHLDMKTKDIIKDALRDFDGTLILVSHDRDFLDGL
                     ATKVFEFGHKRVKEHFEDIKGFLAMKKMESLREIEK"
     gene            5975..7198
                     /locus_tag="P5335_02675"
     CDS             5975..7198
                     /locus_tag="P5335_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRZ73818.1"
                     /translation="MPLIDYSFLGKIIPQQKHKKSYQKAKQSYLNRMRLAMSFFYFGM
                     GFCFASWASRIPDIKSFLNLSEGELGTILFALPIGQLSAMPFSGKIVTRFGSRKVLLF
                     ALPFYAFSLTNIGLAEQPWQLALGLYLFGICGNFSNISINTQGVYTEQIFKKAIMGSF
                     HGSWSLAGFCGALVGLVMMAFKLTPYQHFLIVFALVGVIIFINYRNLAKVKVVKSDSF
                     ELKPRFGKPDKSLVWLGVIGFCCMASEGIMFDWSGVYFKDIVKAPGALVILGYTSFMV
                     TMASGRFLSDILVRKFGAKKILLFSGITISMGLYTAVIFPYLIPSTIAFMMVGFGVSN
                     VIPIVYSTTGRITTLPTGEALTIVTGISFLGFLMGPPIIGHIAELTSLRYSFAFIGMF
                     GVFISVLAYYLKIFR"
     gene            complement(7202..7420)
                     /locus_tag="P5335_02680"
     CDS             complement(7202..7420)
                     /locus_tag="P5335_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRZ73819.1"
                     /translation="MGMIKIFSGSEILASALNEKIEAIGVDVLKKDNIQSARLGGFGN
                     SDLAVELFIDERYYGKVNDVIEEFRMSI"
     gene            complement(7460..8071)
                     /locus_tag="P5335_02685"
     CDS             complement(7460..8071)
                     /locus_tag="P5335_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRZ73820.1"
                     /translation="MQLKKINPKLQQALIENGLTEANELQHETFSTLKSGVDAVIQSP
                     PKSGKTTTLVLNVIQRLEKAIGESTRALVMVENRDKVLETVELFQKLGNHLDLRVIGV
                     HEKGDIDYDKNQISLGMDILVGTPTKINDMFSSAGFNMNTIKLFMVDDAEVMFRNRMD
                     AIILRLSLSVEKTQRVFFCSTITERVEVLADKIMIEPLFFEAE"
     gene            complement(8138..9301)
                     /locus_tag="P5335_02690"
     CDS             complement(8138..9301)
                     /locus_tag="P5335_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRZ73821.1"
                     /translation="MSKILIIEDEAAIRRVLTKILSEENDTYQVEDVEDGIQGLEKIK
                     NEDYDLVLCDIKMPKMDGVEVLEAIKKLKPEIPVVMISGHGDLETAVNTMRLGAFDYI
                     SKPPDLNRLLNTVRNALDRKQLVVENKILKKKVSKNYEMIGESEPINHIKSMIEKVAP
                     TDARVLITGPNGTGKELVAHQLHEKSERASFPIIEVNCAAIPSELIESELFGHVKGAF
                     TSAVKDRAGKFEAADGGTIFLDEIGDMSLAAQAKVLRALQESLIQRVGADKDIKVNVR
                     VLAATNKDLKKEIAEGRFREDLYHRLAVILIKVPALNERRDDIPLLIEHFASKIAEEQ
                     GNSPKHFSLNAINLLKEYDWTGNIRELRNVVERLIILGGNEISENDVKLFASK"
     gene            complement(9367..10674)
                     /locus_tag="P5335_02695"
     CDS             complement(9367..10674)
                     /locus_tag="P5335_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRZ73822.1"
                     /translation="MSVLSLIIKREFIAKVRNKSFIVMTFLSPLLFVGIAVFVGYLAS
                     MKADLKSIAIHDESGYFVNEFKNDAEFSYHDLSKIDINFLKDSIISERYEGLIYFPKT
                     ASLAEIQKSIQYISNDSPSMDYIMDIEEIIAKKITQANYEKIGLDTLKINSAKANVDI
                     VLKKSTGEDTVKGLNEIKLFLGGIFGYLIMMFIVIYGNFVMRSVIEEKTNRIIEIIIS
                     SVKPFQLMMGKIIGNSLAGILQFLIWTILGLVLFFVFSSLFGMQMGASTTVTPDMVAS
                     ANPNGFAEKLPLYINEFKQLPLGTLLVSFVIYFIGGFFLYSSFYAAIGAAVDNETDSQ
                     QFLLPIILPLILGVYIGFFTVLNDPHGTVATVFSIIPLTSPIVMLMRIPFGVPWWQLV
                     LSMVILYATFLFVVWFAAKIYRVGILMYGKKPSWKELYKWLKY"
     gene            complement(10674..11597)
                     /locus_tag="P5335_02700"
     CDS             complement(10674..11597)
                     /locus_tag="P5335_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRZ73823.1"
                     /translation="MSNILEVRQVVKKYGEFTALNSVSLTVPKGSIYGLLGPNGAGKT
                     SLIRIINQITMPDSGEILLDGELLQPHHIQHIGYLPEERGLYKSMKVGEQCLYLAQLK
                     GLSKQEAKKQLDYWFDKLEIQGWWNKKIEELSKGMAQKVQFVVTVLHKPKLLIFDEPF
                     SGFDPVNANIIKDEILELKKNGATIIFSTHRMESVEELCDDIALIHKSNKLIEGKLTD
                     VKRQHRTNSFEVGILTNNVEGLMIDLSQKFKVNQAQFKSLNDDLKLEISIGTSSPNEL
                     LQILTGRGQVTHFVEKIPSANDIFIKTVSES"
     gene            complement(11759..12871)
                     /gene="dnaJ"
                     /locus_tag="P5335_02705"
     CDS             complement(11759..12871)
                     /gene="dnaJ"
                     /locus_tag="P5335_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRZ73824.1"
                     /translation="MKKDFYEILGVTKSASAEEIKKAYRKKAIEFHPDKNPGNKEAEE
                     NFKKAAEAYEVLSDPNKKAKYDQYGHAAFDGNGGFGGGGGMNMDDIFSQFGDIFGSAF
                     GGGFSGGFGGGQRRMKGSNLRIKVKLTLEEIAFGVEKKVKVKRKVQAPGVSYKTCGTC
                     NGSGQVLRVTNTILGRMQTATTCPTCGGSGQSISSKPANADAQGMILEDETVSIKIPA
                     GVVNEMQLKVTGKGNDAPGNGIPGDLIVAIEEIEHEFLKREGENLHYDLYISVAEAAL
                     GVSKEIEAVGGKVRIKLEEGIQSGKILRLKGKGIPSLNSYGNGDLLVHINVWTPKTLN
                     KEQRQFFEKMLTDENFIPHPEKSDKSFFEKVKDMFS"
     gene            complement(12946..13521)
                     /locus_tag="P5335_02710"
     CDS             complement(12946..13521)
                     /locus_tag="P5335_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRZ73825.1"
                     /translation="MFKNFFKQMNQENTENIEKEIIENESTSEQTQEDIEIPVVELTN
                     EEKLQEDLAKEKDKFLRLFAEFENFKKRTAKERIDLFKTANQEVLVAMLPVMDDFDRA
                     MVQISKTEDELLLKGVELIHEKLKSTLVSKGLEQVEISVGDVFNADFAEAITQIPAPS
                     DDLKGKIVDVVEKGYKLGDKIIRFPKVVIGQ"
     gene            13701..14363
                     /locus_tag="P5335_02715"
     CDS             13701..14363
                     /locus_tag="P5335_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRZ73826.1"
                     /translation="MKKIILCLVVLTTLSLTSCKNDKAEAIEPLENAAIAAETATTYK
                     VDTTKSTIEWVGSKPAGKHNGTIALSNGELSLNNGKVESGKFTIDMNSITVLDLKAGD
                     GKEDLEGHLKGLGKVEDADHFFNTKKFPEGTFEITSVATVDAVTTVSGNLTLKGITKP
                     VSFPAMILVDGNTVTLNSETFKIDRTHWNVNYASKSVFDNLKDKFVDDEIELKVSITA
                     TN"
     gene            complement(14418..15323)
                     /locus_tag="P5335_02720"
     CDS             complement(14418..15323)
                     /locus_tag="P5335_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRZ73827.1"
                     /translation="MKILITGATGMVGKELVSLLLQNGITVHYLTTSKDKIINKPNYY
                     GFYWSPQEGYIDENCLMGVESIIHLAGATVSKRWTNNYKQEIIESRIISSSLLYNCLK
                     NHPNQVKQLVSASAIGIYPNSLTLVYTEENTTSDDSFLGNVVVKWEESVDVFKVLNIK
                     VCKLRIGLVLSNKGGALLEMLKPIQVGLGSAFGSGKQVQSWIHVEDLANMFFYAVQNQ
                     WYGAYNAVAPNPVTNSELIKTIAKTVNKPLFMPNIPKFVMSLVLGEMHQLLFSSQHVS
                     SRKAEKNGFQFRYRDLAKTLDNLLS"
     gene            15571..17517
                     /gene="thrS"
                     /locus_tag="P5335_02725"
     CDS             15571..17517
                     /gene="thrS"
                     /locus_tag="P5335_02725"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRZ73828.1"
                     /translation="MIKITLPDGSVKEFSSGVTPMDVAKSISEGLARNVISASFNDTT
                     IETETSLTTDGALVLFTWNDKEGKKAFWHSTSHVMAQVLEELYPGIKLTLGPAIENGF
                     YYDVDFGDKKIVEADFKAIEDRILEIAREKHEFRMRPVSKAEALSIYKNNEYKTELIS
                     NLEDGTITFCDHSNFFDLCRGGHIPNTGIIKAMKIMSVAGAYWRGDEKNKQLTRVYGI
                     SFPKQKDLTDYLELLEEAKRRDHRKLGKELDLFAFSQKVGQGLPLWLPKGAALRDRLE
                     QFLKKAQKKAGYEQVVTPHIGQKELYVTSGHYAKYGADSFQPIHTPAEGEEFLLKPMN
                     CPHHCEIYNTRPWSYKDLPKRYAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCTPD
                     QLDSEFKKVIDLVLYVFNSLGFENFTAQISLRDKENTEKYIGSDENWEKAENAIINAA
                     RDKGLNTVVEYGEAAFYGPKLDFMVKDALGRQWQLGTIQVDYNLPERFELSYKGADNE
                     MHRPVMIHRAPFGSMERFIAILLEHTAGNFPLWLMPEQAIILSLSEKYENYAQKVLSL
                     LENHEIRGLIDNRNETIGKKIRDAEVKKIPFMIIVGEEEEKNGTISIRRHGQEGRGNS
                     TVTIDEFAKIVNEEISKTLKPFDV"
     gene            17541..18089
                     /gene="infC"
                     /locus_tag="P5335_02730"
     CDS             17541..18089
                     /gene="infC"
                     /locus_tag="P5335_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014726496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRZ73829.1"
                     /translation="MRNNRGYQPRVEKKAAHRINNLIRVPEVRLVGENIEPGVYKISE
                     ALRLAEEFEVDLVEISPNAEPPVCKLMDYGKFIYEQKKREKMLKAKSTQIVVKEIRFG
                     PQTDEHDYEFKKKNAEKFLKEGAKLKAFVFFKGRSIIYKEQGQILLLRLAQDLEELGK
                     VEALPVLEGKRMIMYLAPKKKK"
     gene            18122..18319
                     /gene="rpmI"
                     /locus_tag="P5335_02735"
     CDS             18122..18319
                     /gene="rpmI"
                     /locus_tag="P5335_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRZ73830.1"
                     /translation="MPKMKTKSSAKKRFKVTGSGKIKRKHAFKSHILTKKSKKRKLAL
                     THSTLVHKTDEKSIKQQLRII"
     gene            18446..18790
                     /gene="rplT"
                     /locus_tag="P5335_02740"
     CDS             18446..18790
                     /gene="rplT"
                     /locus_tag="P5335_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRZ73831.1"
                     /translation="MPRSVNSVAKRARRKKVLKQAKGFFGRRKNVWTVAKNAVEKAMV
                     YAYRDRKQNKRNFRSLWIMRINAGARLEGMSYSQFIGKVKANGIELNRKVLADLAMNH
                     PEAFKAILNKVK"
     gene            complement(18871..19716)
                     /locus_tag="P5335_02745"
     CDS             complement(18871..19716)
                     /locus_tag="P5335_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRZ73832.1"
                     /translation="MAKIIIHGGFFSESSTNLETKIAKQEALLRIVKDSYEYLTTHSA
                     LETVVYAVSLLEDDELFNAGIGSQIQSDGKIRMSAAIMDGTSLKMSGVINIEEVKNPI
                     QVAEVLLNVDDRVLGGKGATSFARKHGFEPFSTEIPQRRAEYEAKLQATGIGTVGCVA
                     LDSNGKIAVATSTGGKGFEIPGRISDSATVAGNYANEFCGVSLTGVGEDIVSGAVATK
                     IVTRVTDGFTLEAAFEKTFNELKPFDGFAGAIAIDKEGTIFHKDSHPSMVFASFDGDK
                     VEVFM"
     gene            complement(19808..>19992)
                     /locus_tag="P5335_02750"
     CDS             complement(19808..>19992)
                     /locus_tag="P5335_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HRZ73833.1"
                     /translation="AIGSGLVILVDGREIKDTNLTQVELGQPISINHLVTHVMSMHDT
                     FDLETYKMHIHSSQYV"
     assembly_gap    19993..20092
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20093..20835)
                     /locus_tag="P5335_02755"
     CDS             complement(<20093..20835)
                     /locus_tag="P5335_02755"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HRZ73834.1"
                     /translation="MNSRGKLIIIGGAVDKGSFTESSFDQDVSKNMNFFEKGILKRII
                     DESKYKEKSRIEIITTASKIPREVGPEYAKAFQYLNATNVDVLHIERREQASEQENID
                     RLKAADIVMFTGGDQLRLSSILGGTKFHDILLDKYKNELFIYAGTSAGAAAASNNMIY
                     QGSSHEALLKGEVKITSGLGLIDDVIIDTHFVQRGRIGRLFQAVVGNPKVLGIGLGED
                     TGLLITHNSKMEAIGSGLVILVDGREIKDT"
     gene            21122..>23739
                     /gene="cphA"
                     /locus_tag="P5335_02760"
     CDS             21122..>23739
                     /gene="cphA"
                     /locus_tag="P5335_02760"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRZ73835.1"
                     /translation="MKILKVQALRGPNLWSIQRKKLIQMRLDLEEMEQNPTNKIDGFR
                     ERLEAMFPAMIEHRCSEGVRGGFFMRIEKGTWMGHVIEHIALEIQTLAGMETGFGRTR
                     ETKTPGVYNVVFSYTEENVGMFAAESSVRIAEALIAGTDYDLEADIKKMKEIRERVRL
                     GPSTGSIVEEAVSRDIPWIRLGTNSLVQLGYGVNQMRFQATITCKTSNIAVDIACNKE
                     ETKRMLELASIPVASGSICVDEEDLELTIKKIGYPIVIKPLDGNHGKGASINVVTWED
                     AKEGLEHAKKYSRRVIVEKFITGFDFRVLVIDNKLVAAAKRVPAHVVGNGKNTIQELI
                     DITNLDPKRGYGHENVLTEITVDRDTTDLLEKMGYTLETLPNNGETVYLKSTANLSTG
                     GTSVDVTDMMHPENIFLAERISRVIGLDVCGIDIMAENLTQPLKENGGVILEVNAAPG
                     FRMHLDPSEGLARNVAAPVIDMLYPPGKPSRIPIIAVTGTNGKTTTTRLLAHIVKNNG
                     FRVGFTTSDGIYIQNHMLEKGDTTGPLSAEYILKDPTVEFAVLETARGGILRAGLGFS
                     RCDIAIITNIREDHLGLNDIDTLDDLARVKSVVVRSVKKDGWAILNAEDDQCMKIAND
                     LNCNIAYFSMDEENPVAKKLSKEGKIVAVYENGFVTIKKGDWKIRIEKVLHIPLTLGG
                     KAKFMIQNVLAATLASYLYGFKTDDISLSLQTFIPSVAQTPGRMNIFEFKRFKVMIDF
                     AHNPAGYSAIEDLLSNIDATRKIGIIAGVGDRRDEDIRDCAEIAARMFDHIIIRQEKH
                     LRGRTEQEIIDLILEGIQLANREVTYEIIPKEIEAIKHAINIADEGTFITALSDVVTN
                     AIGIVQEYLDKENEEN"
BASE COUNT         7847 a         4130 c         3952 g         7710 t
ORIGIN      
        1 aactcacgtt caaactatca ctgttcagga tactactgct cctacttttg tagaaacatt
       61 accgcaagat acttcagctg aatgtagtaa tattcctgtt gcagcaacat taactgcaac
      121 agatagttgt ggagacgcaa cagttactta tacagaggca attgttcaag gtgaatgtac
      181 cggagatttt gttatcacaa gaacatggac agcaacagat ttatgtggaa atagcgtatc
      241 acacgaacaa ataattattg ttcaagatac aataggacct gtatcctctc cttttgatga
      301 aaacattagt gttacttgtg aagcaattcc tgtaagtcca gatttaacct ttacagatgc
      361 ttgttctaca gttggtggag taacattcaa tgaggaaaca gtaaatgaat cagctacttc
      421 ttatacaata gtaagaactt gggaagctta tgatagttgt ggtaatttat caacctttac
      481 acaaactatt aatgtaacaa ttcaagatag ttttacacca atttctagta catcatgtat
      541 tggagatagt actcctattg accttaatac attattacca acaggaacta caggtggaac
      601 ttggattgat gtaaataata ctggtgcatt aaataataat atctttacac ctactcttgt
      661 agcggctgtt ggaaattaca tctttagata tgaaatcact ggaggttctt gtccaagagt
      721 atatgaagtt acaatgaatg taaatgctga ttgtattgtg ttcccttgtg atgctattaa
      781 tatttataat gcgatgtctc caaatggaga tggcttgaat gaatttttca atattgaaaa
      841 cattgaaaat actgaatgct atccaagcaa ttcagttgaa atctataacc gttggggcgt
      901 gttagtgtat gatgttcaga actatgataa tagttccaaa gcattccgag gtgtttctga
      961 aggtagagcg acagttaata agtctgctga attaccaacc ggaacctatt tctatatcat
     1021 tcagtatact acatttgatg gagatgttgt aaataaatct ggatatttgt acctaacaag
     1081 ataaaataaa ctcctgaggg tttaacgacc ctcgggtttc taaaaaaaga aactaaatga
     1141 gaacaaaatt tttatttttc gctttgatgt taacaggttt tgctggattc gctcagcaag
     1201 atgctcagta tacgcaatac atgtataaca caataaatgt caatcctgct tatgccggat
     1261 cgagaggagt aatgagtatt ttcggactgc acagaaccca atgggttgga ttagatggtg
     1321 caccggtaac gaatactgcc tctattaaca caccaataaa tgagagtaat ctaggtttag
     1381 gtttatcctt tgtaaatgat cgaataggtc caacaaacga gaacactatt tcagcagatt
     1441 tatcttactc cattaatacc tctgaaacct acaaactttc gtttggtatt aaagcatcgg
     1501 caaatttatt taacttggat gtaaataagt taaatccagc tgatcaagga gatccacagt
     1561 ttgcagatct agaaaataag ttcactccta atattggagc cggtgtttat ttccattcgg
     1621 ataaaacata ctttggtctt tctgttccta gcttttttga gactacccgt tatgatgaca
     1681 actctgttgc aatcaataaa gaaagaatga gtttttattt tattgcaggt catgttttcg
     1741 atttaagtta caatcttaag tttaaacctg ccttaatgac taaagctact gaaggtgctc
     1801 cattacaagt agacttatcc gctaatttcc taataatgga taaatttgtg ttaggtgctg
     1861 cttggagatg ggatgctgcc gttagtgcaa tggcaggatt tcaagtttcc gacggattat
     1921 acataggata tggctatgat atggaaacaa cagaattaaa aaactacaat tcaggttcac
     1981 atgaagtatt tttacgcttt gaattgttcc aaagacataa caaaattgtt tcaccaagat
     2041 tcttctaata agcaacagtc atgaaaataa aatttatata catcacttta ttaagttgta
     2101 tcgctttaaa tgtttcagcc caaaaagcgg gagtaaaaaa agcagataag aactatgata
     2161 aattcgccta catagatgca attaaaacct atgagcgagt ggccgaaaaa ggctttaaaa
     2221 cgcctgatat gttgcaaaaa attggaaatg cctactatta caatgccgat ttagtgaacg
     2281 ctgaaaaatg gtatggtgaa ctttttgcaa tgaccgaaga agttgaacct gaatattttt
     2341 tccgttacgc acaatcatta aaaggagttg gcaattacgc taaagctgat gaaatgatgg
     2401 caaaatttaa tctgaaaagc ggaaatgact caagaggttt aatagcgaaa actaaaaaag
     2461 attaccgcga agaaatcaag aaaaactcag gtcgatataa aattgaagat gccggaatca
     2521 attcggaata ttcagattat ggtcctgcat tttatctgga caaggtggtt tttacatcca
     2581 ctcgtgatac aggtaatttt tcgaaaagaa aacacagttg gactgaccaa tatttttcaa
     2641 atctatattc agcagatatg actactgaag gcgttttatc ttctgcagga aaatatggta
     2701 agaaattgaa tactaaattc cacgaatcaa ctcctgccta tactaaagat ggtaagacag
     2761 tttattttac ccgtaataat ttcttagatg gtaaaaaagg aaaagatgca aacaaggtta
     2821 ctttattaaa aatatataaa gctactcttg aagaagataa atggataaat atcactgctt
     2881 taccttttaa tagcgatagc tatcaagttg ctcatccaac gttgagtgct gatgaaaaaa
     2941 cattatattt tgcctctgat atgccaggaa caaaaggctt atctgatctt tataaagtag
     3001 acatcaaaga agatggttct ttcggaacgc ctgtaaactt gggctctgac atcaacacag
     3061 aaggtcgtga aacattcccg tttattactt cggacaatga attgtatttt gcttctgatg
     3121 gtcatccagg attaggtgga ttagacattt tcataactaa attagaaaaa gacggttcgt
     3181 ttaaacaagt tcataatgtt ggagaatctg caaatagtca atatgatgat tttgccctga
     3241 ttatcaataa aacttcaaaa agaggatttt taacttcaaa cagaccgggt gttggaaatg
     3301 acgatatcta taaattctta gaaactcgtg aaattgattg ggattgtgag caatcactag
     3361 taggtattgt tacagatgca aatacaggtg caattcttcc aaacacaaaa ctgacattat
     3421 ttgatgaaaa attcactaag ttaagagata ctatttctgg ccttgatggt cgttatgatt
     3481 ttggaattgt cgattgtgag aaaaaatatt atgtaagagc tgaaaaagaa gcttacacaa
     3541 caaaagaagt tccggtaata attgccaaag aaagcggtat aacagaatta ccaattgaat
     3601 tagacagtgc tgttaaagca attcctgtag gaggtgattt agctgacgta tttggaataa
     3661 acctcatcta ttttgattta gacaaatgga acataagacc agatgctgcg atagatattg
     3721 ctaaaatttt agatgtatta aaccaatatc caacgatgaa aattgatatt cgttcacaca
     3781 cagatagtcg tcaaactcat aaatacaatg aagtattgtc tgaccgtagg gcaaaatcaa
     3841 ctagagattg gttaatcaaa cacggaatag ctccggaacg tttaacagca aaaggctatg
     3901 gagaaaccca attggtaaac aaatgtgctg ataatgtaga atgctctgaa gctgaacacc
     3961 aattaaacag aagaagtcaa ttcattatta ctgatttata atactgttgt taattcattt
     4021 aaaaaaaata tcccgttaat ttttaacggg atatttttat ttataaaaca ataataaact
     4081 ttttcctatt tttcaatctc ccttaaactt tccatttttt tcatagctaa aaatcctttg
     4141 atgtcttcaa aatgttcttt cacgcgttta tgaccaaatt cgaaaacttt ggtagccaat
     4201 ccgtcaagga aatctctatc gtgggaaact aaaatcaaag tcccatcaaa atcacgtaaa
     4261 gcatctttga tgatgtcttt ggttttcata tctaagtgat tggatggctc atctaaaatc
     4321 aataaattca ctggttccaa caataattta atcattgcca accgtgtttt ttctccaccg
     4381 gacaaaactt ttactttctt ggtaatatca tcaccttgga acatgaatgc tcctaaaata
     4441 tttttaattt gtgtgcgaac ttcaccaaca gcaatatcat caatcgtttc aaaaatagta
     4501 gcattttcat ccaacatcga agcttggttt tgagcaaaat agcctatttg tgcattgtga
     4561 ccaatctcaa cacttccgcc attgatttca atttctttca taatggcttt aatcatggtt
     4621 gattttcctt cgccattttt gcccacaaaa gctactttat caccgcgttc aatcacaata
     4681 ttggcatctt taaaaatcaa atgatcaccg tatgattttt ccaaatcttt caccaccacc
     4741 ggatattgac cagaacgtaa agaagatggg aatttcaatt tcaaagccga attatcaatt
     4801 tcgtcaattt caaccggaac taatttttct aacattttca cacgcgattg tacttgcaac
     4861 gtttttgaat acgttccttt aaatcgctca ataaactcca tattatcagc aatcattttt
     4921 tgctgttcat cataggcttt ttgttgatgc actcttcggt ctttacgcaa ctccaaataa
     4981 tgcgaatatt tggctttgta atcataaata cgacccatcg ttacttcaat agtacgattg
     5041 gtgatattat cgacaaaagc tctatcatgt gaaatgacca tcaccgcttt ggccgaattg
     5101 attaaaaact cttctaacca ttgaatactt tcaatatcca tgtgattggt aggctcatcc
     5161 aataaaatca aatcgggttt tttcaaaaga atcttggcca attcaattcg cattctccag
     5221 ccaccggaaa attcagaagt gggtcggtta aaatcttcgc gttcaaaacc caaacctttt
     5281 aatacttttt ctacctcagc ttcatagttg acttcttcaa tagaataata tttctcactg
     5341 acttcagaca cacgctcgat taatttcata taatcgtcgc tttcataatc ggttcgaata
     5401 gtcaattgtt cattgagatg atcgatttca tttttcatgt caaagatttc tgcaaaagct
     5461 ttcgaagctt cttccatcac cgtcgcatca tctttcgtta ataaatgctg tggcaaatag
     5521 gcaatcactg catcttttgg agctgaaatg tttccggttg aagcctttgc ttctccggcg
     5581 ataattttca atagtgtcga tttaccggca ccgtttttac ccatcaaggc aattttatca
     5641 ttttcattaa tagcgaaaga aacgtcgcta aacaaagtgg ttccgccaaa atgaacggca
     5701 atatcgttaa ctgtaatcat atttttaagt tggaagttag aagatggaaa ctggaagtca
     5761 gttcacattt ctgatttttc aaggtgcaaa gataaccgat ttgtggaatc cgaatgtttg
     5821 aaaatgataa attgtaccac atttaatttt ggatatcata attcaacaag acaattcttg
     5881 aaaaatggat aattagtttg ccaaaggcat acaaatcaag ttctaacact atttttttaa
     5941 ttaatttcgt agtctcaatt agttaataaa aaagatgcct ttaattgatt acagtttttt
     6001 aggaaaaatt attccgcagc aaaaacacaa aaaaagctat caaaaagcaa aacaatccta
     6061 tttgaaccgg atgcgtttgg cgatgtcgtt tttttacttc gggatgggtt tttgttttgc
     6121 ttcatgggca agtagaattc ctgatatcaa atctttttta aatttaagcg aaggtgaatt
     6181 gggaactatt ttgttcgcac tccctattgg acaactttct gccatgcctt tctccggaaa
     6241 aattgttact cgttttggaa gtagaaaagt attgttattt gcattacctt tttatgcttt
     6301 tagtttaaca aatattggtt tagccgaaca accttggcaa ttggcacttg gattatatct
     6361 ttttggaatt tgcggaaatt tcagtaatat ttcaattaac acacaaggcg tttacaccga
     6421 acagatattt aaaaaagcaa tcatgggttc gtttcatggt tcttggagtt tagcgggatt
     6481 ctgtggagct ttggtaggtt tggttatgat ggcttttaaa ttgacgcctt accagcattt
     6541 ccttattgta tttgcccttg tcggagtaat catctttatc aattatcgaa atctggctaa
     6601 agtcaaagtg gtaaaaagtg attcatttga attaaaaccc cgatttggaa aacccgataa
     6661 atcattggtt tggttgggtg taatcggttt ttgttgtatg gcaagtgaag gaattatgtt
     6721 cgattggagt ggagtatatt ttaaggacat tgtaaaagct ccgggagctt tggtcatttt
     6781 aggctataca tcattcatgg ttactatggc ctccggacga tttttaagtg atattttagt
     6841 gcggaaattt ggtgcaaaaa aaatattact atttagtgga attactattt cgatgggatt
     6901 gtatactgct gtaatttttc cgtatttgat tccaagtaca atcgcattta tgatggttgg
     6961 atttggggtt tctaatgtca ttcctattgt ttacagcact acgggacgaa tcacaacttt
     7021 accaacaggg gaagcattaa cgattgtgac cggaataagt ttcttaggat ttttaatggg
     7081 accaccaatc attggtcata tcgccgaatt aaccagtttg cggtattcgt ttgcttttat
     7141 tggaatgttc ggagtgttta tatcggtctt ggcgtattac ttgaaaatat ttagatgaga
     7201 attaaatact cattctaaac tcttcaatta catcattgac tttgccataa taacgttcgt
     7261 caataaataa ctcaacagct aaatcagaat ttccgaaacc acccaagcgt gctgattgaa
     7321 tattgtcttt ttttaaaaca tcaaccccga tagcttctat tttttcattt aatgctgaag
     7381 ctaaaatttc acttcctgaa aatattttga tcatgcccat tttgtaaagg ttaagtgtta
     7441 aaagtaattg gtaataagtt tattccgctt caaagaaaag cggttcaatc ataattttat
     7501 ctgctaaaac ttctacacgt tctgtgatag ttgaacaaaa aaagacgcgt tgcgttttct
     7561 ctacactcaa agaaagtctt aaaatgatag cgtccattcg gtttctgaac ataacttcgg
     7621 catcatcaac cataaatagc ttaattgtat tcatgttaaa tcctgcagaa gagaacatat
     7681 cgtttatttt agttggcgtt ccaactaaaa tatccatacc caaagaaatt tgatttttgt
     7741 cataatcaat atcccctttt tcatgcacac caataacacg caaatctaaa tgattgccta
     7801 atttttgaaa taactcgact gtttcgagca ctttatcacg attttcaacc ataactaagg
     7861 ctcttgtgct ttcaccaatc gctttttcta atcgctgaat aacatttaaa acaagcgtag
     7921 ttgtttttcc gcttttaggg ggcgactgta taacagcatc tactccactt tttaaagttg
     7981 aaaaggtttc atgctgtaat tcattcgctt ctgttagacc attttcaatt aaagcctgct
     8041 gaagcttggg atttattttt tttaattgca tatttcattt ttaaggtaat ggatgattgt
     8101 taaagggatt ttttttcgcc tactaccaat taccatttta cttactcgca aatagtttta
     8161 catcattctc agaaatttca tttcctccca atatgattaa acgttctacc acgttgcgaa
     8221 gttcgcgaat atttccggtc caatcgtatt ccttcaataa attaatagca tttaatgaaa
     8281 aatgttttgg agaattacct tgttcttcag caattttaga agcaaaatgt tcaattaata
     8341 ggggaatatc atctcttcgt tcatttagag caggaacctt aattaaaatg accgccaatc
     8401 gatggtatag atcttcacga aaacgacctt cggctatttc cttttttaaa tctttatttg
     8461 tagctgccag cacacgaaca ttcactttaa tatctttatc agcacctacc cgttgaatta
     8521 aactttcctg aagtgcacga agtactttgg cttgtgctgc taaactcata tcaccaattt
     8581 catctaaaaa aatggttccg ccatctgcag cttcaaattt tccggctcgg tcttttactg
     8641 ctgatgtaaa agctcctttc acatgtccga ataactcact ctcgattaat tccgaaggaa
     8701 tggcagcaca attcacttca atgattggaa aagaggctcg ttcacttttt tcatgtaatt
     8761 gatgagccac taattctttt ccggtgccgt ttggacctgt aattaatacc cgtgcatcgg
     8821 ttggagccac tttttcaatc atgcttttga tatgattaat aggctctgac tcccctatca
     8881 tttcataatt tttactgact ttctttttca gtattttatt ttcaacaacg agttgttttc
     8941 tgtctaaagc atttcgaaca gtgttcaata atcgatttaa atcgggtggt ttcgaaatat
     9001 aatcaaatgc ccccaaacgc atggtattta cagctgtttc caaatctccg tgtccggaaa
     9061 tcatcactac cggaatttct ggtttcaatt ttttgattgc ttccaatact tcaactccat
     9121 ccatttttgg cattttaata tcgcaaagta ccaaatcgta gtcttcgttt ttaatttttt
     9181 caagaccttg aattccgtct tcaacatctt ctacttgata ggtatcattt tcttccgaaa
     9241 gaatctttgt caaaactctt cggatagcgg cttcatcttc aatgattaat atttttgaca
     9301 ttttttttgg tgctaaggta ctgatgtact gaaaaacgta tgttattatt tatttaaaaa
     9361 atgaggttaa tacttcaacc atttatataa ttctttccaa cttggttttt tgccatacat
     9421 taagattccg acacgataaa tttttgcggc gaaccacacc acaaataaaa aggttgcata
     9481 caatataacc attgacaaaa ccaattgcca ccaaggtact ccaaatggaa ttcgcatcag
     9541 catcacaatg ggtgaagtta acggaataat tgaaaacact gttgcaacag ttccatgtgg
     9601 gtcatttaaa acagtgaaaa aacctatgta aactcctaaa attaacggta aaataattgg
     9661 caataaaaac tgttgtgaat cagtttcgtt atccacagct gcccctatgg cggcataaaa
     9721 tgaactgtat aaaaagaaac ctccaataaa atatatgaca aatgaaacca aaagcgtacc
     9781 tagtggtaat tgtttaaatt cattgatgta taaaggtaat ttctctgcaa aaccattcgg
     9841 atttgctgaa gctaccatat cgggagtaac tgttgttgat gctcccattt gcatgccgaa
     9901 taaagaagaa aacacaaaaa atagcactaa tcctaaaatt gtccaaatta aaaattgtaa
     9961 aattccggct aatgaatttc cgatgatttt acccatcatc aattgaaatg gtttcactga
    10021 tgaaataatg atttcgatga tacgattcgt tttttcttca atgacacttc gcatgacaaa
    10081 gttgccgtaa attacaataa acatcatgat taaatacccg aaaatacctc ccaaaaacaa
    10141 tttgatttcg ttcaaacctt tgacagtatc ttctccggta gattttttca aaacaatatc
    10201 tacatttgct ttagcactat tgatttttaa agtatccaat ccaatttttt cataattggc
    10261 ttgcgtgatt ttcttagcaa taatttcttc aatatccatg atataatcca ttgatggact
    10321 atcatttgaa atatattgta tgcttttttg aatctcagct aagctagctg tttttgggaa
    10381 atatatcaac ccttcataac gttcgctgat gatgctatcc ttcaaaaaat ttatatctat
    10441 ttttgacaaa tcgtgatacg aaaattcggc atcattttta aattcattca caaaataacc
    10501 actttcatca tgaatggcaa tgctttttaa atcagctttc attgaagcca aataacccac
    10561 aaaaacagca attcccacaa acaatagcgg actcaaaaaa gtcatgacaa tgaatgattt
    10621 gttgcggact ttcgcaataa actctctttt gattattaaa gataatacac tcattaggat
    10681 tcacttacgg ttttaataaa tatatcattg gcagatggga ttttttcaac aaaatgggtc
    10741 acttgtcctc taccggtcaa aatttgaagt aattcattag gacttgaagt tccaatggag
    10801 atttctagtt ttaaatcatc gtttaacgat ttaaattgtg cttgattaac cttaaacttt
    10861 tgcgataaat caatcattaa tccttccaca ttattggtta aaattccgac ttcaaaacta
    10921 ttggttcgat gttgtcgttt cacatcggtc aattttccct cgattagttt attcgattta
    10981 tgaatcagag caatatcgtc acataattct tctacacttt ccattcgatg agtcgaaaaa
    11041 attatagtag caccattttt tttcaactcc aaaatttcat ctttaataat attggcattt
    11101 acaggatcga aacccgaaaa tggctcatca aaaatcaata actttggttt atgcaaaaca
    11161 gtaactacaa attgtacttt ttgagccatt cctttggaaa gctcttcaat ttttttattc
    11221 caccaacctt gaatttctaa tttatcaaac caataatcca attgtttttt ggcttcctgt
    11281 tttgataatc ctttgagttg agccaaatat aaacattgct cacccacttt catcgattta
    11341 tacaaacctc gctcttctgg aagatatcca atatgttgga tgtgatgtgg ttgaagcaat
    11401 tcgccgtcta acagaatttc accactgtca ggcattgtaa tttgattgat gatacggatt
    11461 aatgatgttt ttccggctcc gtttggacct aataatccat aaatactacc tttcgggacc
    11521 gttaaagaaa cactattgag tgcggtaaat tcgccgtatt ttttgacgac ttggcgtact
    11581 tccagaatgt tgctcatacc tatttttgag ttttggtaaa tatacggaat tagtacattt
    11641 ttagctacgt ttgttacaaa aaaaacccac ccttatttgc ataaggatgg gaaaaattgc
    11701 tatgaaaaag aatctactag tttcctagta aagttcaaat gtaaatattt ttttcatatt
    11761 atgaaaacat atctttaact ttttcaaaaa aagatttatc tgatttttca ggatgtggaa
    11821 tgaagttttc atccgtcagc attttctcaa aaaattgacg ttgctctttg tttaaagtct
    11881 ttggtgtcca aacatttata tgaactaata aatcaccatt cccgtatgaa tttaaacttg
    11941 gaattccttt tccttttaat cttaaaattt tacctgactg tattccttct tctaatttga
    12001 ttctcacttt tccaccaaca gcttcaattt ctttagaaac acctaatgca gcctcagcca
    12061 cgctgatgta taaatcataa tgaagatttt ctccttctcg ttttaaaaat tcatgttcaa
    12121 tttcttcaat agcaacaatt aaatcacctg gaattccatt acccggagca tcgtttcctt
    12181 tacctgttac ttttaattgc atctcgttaa caacaccggc aggaatttta atcgaaacgg
    12241 tttcgtcttc caaaatcatt ccttgtgcat cggcatttgc tggttttgaa ctgatagatt
    12301 gacctgaacc accacaagtt ggacaagtag ttgccgtttg cattctacct aaaattgtat
    12361 tggttacacg taaaacctga cccgaaccat tacaagttcc acatgtttta taagaaacac
    12421 cgggagcttg aactttccgt ttaactttta cttttttctc aactccaaag gcaatttcct
    12481 ctaatgttaa tttaactttt attcgtaaat tgctaccttt cattctgcgt tgaccaccgc
    12541 caaaacctcc actaaatcct cccccaaaag cacttccgaa aatgtcgcca aattgactga
    12601 aaatgtcatc catgttcatt ccgccaccgc cgccaaatcc accattgcca tcaaatgcag
    12661 catgaccata ttgatcgtat tttgcttttt tattaggatc gctcagcact tcataagctt
    12721 cagctgcttt tttgaaattt tcttctgctt ctttatttcc cggattttta tctgggtgaa
    12781 actcaattgc tttcttgcga taagctttct taatttcctc agccgatgca cttttagtaa
    12841 cgcctaatat ttcgtaaaaa tcttttttca tgttttaaaa taccaaattt attcaattca
    12901 atttcctatt ttaattactt catacggata ggtcgagaac tgtctctatt gaccgataac
    12961 aactttagga aaacgaatta ttttatcacc caatttatat cctttttcaa cgacatctac
    13021 aattttacct ttcaaatcat ccgatggagc aggaatttgt gtaatggctt cggcaaaatc
    13081 agcattgaaa acatcgccaa cactaatttc cacttgttct aatcctttag aaactaaagt
    13141 agactttaat ttttcatgaa tcaattcaac acctttcaac aataattcat cctctgtttt
    13201 agaaatttga accattgctc tatcaaaatc atccataaca ggcaacattg caaccaaaac
    13261 ttcttgattg gccgttttaa acaaatcaat tcgctcttta gcagttcttt ttttgaagtt
    13321 ttcaaattct gcaaaaagac gcaaaaattt atctttttct ttagctaagt cttcctgtaa
    13381 tttttcttca tttgtcaatt caactacagg aatttcaata tcttcctgag tttgttctga
    13441 agttgattca ttttcaatga tttccttttc tatattttca gtattctctt gattcatctg
    13501 cttaaaaaaa tttttgaaca ttcgttgtta aattttggat tgcaaaagta ctgccatttg
    13561 tttaaaaatg tcaaattgtc agcgtaaata atttttttaa tcgattttct tcaaaatcaa
    13621 tgtgccaaaa tttaatattt ttttatgaaa aatgggcgtg ttggttttct atttttgttc
    13681 aaacttaaaa ctaaaaaact atgaaaaaaa tcattttatg cttagtagtc ctaactacac
    13741 tttctttaac ttcttgtaaa aatgacaagg cagaagcaat agaaccgtta gaaaatgcgg
    13801 caattgctgc tgaaacagcc acaacgtata aagtagatac taccaaatct accatcgagt
    13861 gggttggctc taagccagcc ggaaaacata atggaaccat tgctctttca aacggagaat
    13921 tgtctttaaa taatggaaaa gtggaaagcg gtaaattcac cattgacatg aacagtatca
    13981 ctgttttgga tttaaaagcc ggtgacggaa aagaagactt agaaggccac ttaaaaggat
    14041 taggaaaagt agaagatgca gatcatttct ttaatactaa aaaattcccg gaaggaactt
    14101 ttgaaattac atctgttgca acggttgatg ccgttactac tgtttccggt aatttaactt
    14161 taaaaggaat tactaaacca gtttctttcc ctgctatgat tttagtagat ggaaatacag
    14221 tgacattaaa tagtgaaaca ttcaaaattg acagaacaca ttggaatgta aattatgctt
    14281 caaaatctgt atttgacaat ttaaaagaca aattcgtaga cgacgaaatc gaattaaaag
    14341 ttagcattac tgcaacaaat taattattat ttcatcttac actttataag aagctcctca
    14401 attgaagagc ttttttttta tgacaacaaa ttatctaatg tcttggctaa atcacggtat
    14461 cgaaattgaa aaccgttttt ttcagctttt cgagagctaa catgttgact ggaaaaaagt
    14521 aattggtgca tttcccctaa aactaatgac atcacaaatt ttggaatatt tggcataaac
    14581 aagggtttat ttaccgtttt agcaatagtc ttaattaatt ctgaattggt tacaggatta
    14641 ggagctacag cattatacgc tccataccat tggttttgaa cagcataaaa aaacatgtta
    14701 gctaaatctt ccacgtgaat ccacgattga acttgcttac ccgagccaaa tgccgacccc
    14761 aatcccactt gaattggttt tagcatttct aataaagctc ctcctttatt tgataaaaca
    14821 aggcctattc ggagcttgca aacttttata ttaagtactt taaaaacatc aacactttcc
    14881 tcccatttta ccactacatt cccaagaaaa ctatcgtctg aagttgtgtt ttcttcggta
    14941 tatactagag tcaaactatt tggatatatt cctatagcag aagcagaaac tagttgcttc
    15001 acttgattag gatgattttt taagcaatta tacaataatg aagaggaaat aatccgactc
    15061 tctattattt cttgtttata attgttcgtc catcgtttcg aaaccgtggc tccggctaaa
    15121 tgaataattg attctacacc cattaaacaa ttttcatcga tataaccttc ttgtggactc
    15181 caataaaaac cataataatt tggtttattt attattttat cttttgaagt tgttaaataa
    15241 tgaaccgtta ttccattttg taataacagg gaaaccagtt ctttaccaac cattcctgtt
    15301 gcacctgtaa ttaatatttt cataatcaat ttttatacaa agttacgttt aagcaattaa
    15361 aatcaatatt aattagcatt agtttaagtg ttgttaactt aagcgaaatc tcgtttttgc
    15421 taaaatcatg gtggatattt ttaaactaaa attgatatta aaaacacaaa acaataaaaa
    15481 acaaaaaaat acttatgagt ataagcaaca tatatattgt atgtgatttg gtaatcttat
    15541 atatttgcat tccattaaaa aacagaagga atgataaaga ttacgctgcc ggatggttcg
    15601 gtaaaagaat ttagctcagg tgtaacgcca atggatgttg ctaaaagcat tagtgaaggt
    15661 ttagccagaa atgtaatttc tgcttctttc aatgatacaa ctattgaaac tgaaacttct
    15721 ttgacgacgg atggagcact tgttttattt acatggaatg acaaagaagg taaaaaagct
    15781 ttttggcatt ctacttccca cgttatggct caagtattag aagaattata tcccggtatt
    15841 aaattgacgt taggtcctgc aattgagaat ggtttttatt atgatgttga ttttggagac
    15901 aaaaaaattg tcgaagccga ttttaaagcg attgaagacc gaattttaga aattgccaga
    15961 gagaaacacg aattcagaat gagaccggtt tctaaagccg aagcattatc gatatataaa
    16021 aataatgaat acaaaacaga attaatttca aatttagaag atggaacaat aaccttttgt
    16081 gatcattcta atttctttga tttatgtcgt ggtggacaca ttcctaatac cggaattatc
    16141 aaagcaatga aaatcatgag tgttgcgggt gcttattgga gaggtgatga gaaaaataag
    16201 caattaacgc gtgtttatgg aatttcattc ccaaaacaaa aagacctaac tgattattta
    16261 gaattattag aggaagccaa gcgtcgcgac catagaaaat taggaaaaga attagattta
    16321 tttgcttttt ctcaaaaagt tgggcaaggt ttaccattgt ggttaccaaa aggagctgct
    16381 ttacgtgacc gtttagaaca atttttgaaa aaagctcaga aaaaagctgg ctacgaacaa
    16441 gtggttactc cacatattgg tcagaaagaa ttatatgtaa cgtcgggaca ttatgcaaaa
    16501 tatggtgccg atagttttca accgattcat acaccggcag aaggtgaaga gtttttgtta
    16561 aaaccaatga attgtcctca tcactgtgaa atttataata caagaccttg gagttataaa
    16621 gatttaccga aacgttatgc tgaatttgga accgtttacc gttatgaaca aagtggagaa
    16681 ttacatggct taacacgtgt gagaggattt actcaggacg atgcacatat tttttgtact
    16741 ccggaccaat tggatagtga atttaaaaaa gtaattgatt tagttttata tgttttcaat
    16801 tcattaggat ttgaaaactt cacggctcaa atatccctac gtgataagga aaatacagaa
    16861 aaatacatag gttctgatga aaattgggaa aaagcagaaa atgccattat caatgcagcc
    16921 agagacaaag gtttaaatac cgttgtagaa tatggtgaag ctgcattcta cggtccgaaa
    16981 ttggatttca tggtaaaaga tgctttggga aggcaatggc aattaggaac aattcaagtg
    17041 gattacaact tgccggaacg ttttgaattg agttataaag gagctgacaa cgaaatgcat
    17101 agaccagtta tgattcatcg tgcacccttt gggtcaatgg aacgatttat agccatttta
    17161 ttagaacata cagcaggaaa cttcccactt tggttgatgc ctgagcaggc tataatcttg
    17221 tctttgagtg agaaatatga aaattatgct caaaaagttt taagtttgct agaaaatcac
    17281 gaaattcgcg gcctgattga caaccgcaat gagacaattg gcaagaaaat cagagatgca
    17341 gaagttaaaa aaataccgtt catgattatt gttggtgagg aagaagaaaa aaacggaacc
    17401 atttcaataa gacgacacgg acaagaagga agaggaaaca gcaccgttac aattgacgaa
    17461 tttgcaaaaa ttgtaaacga agaaattagc aaaacattaa aaccatttga cgtttaactt
    17521 taaattaaag agccatagca attagaaaca acaggggtta ccaacccaga gtagaaaaaa
    17581 aagcagcaca tcgaattaac aatttaattc gtgtacctga agttcgtttg gtaggtgaaa
    17641 acatcgagcc gggagtttac aaaatttctg aagcattgcg tttagctgaa gaatttgagg
    17701 ttgatttggt ggaaatttct cccaatgcag agcctcctgt ttgtaaatta atggactatg
    17761 ggaagtttat ttacgagcag aagaaaagag aaaaaatgct caaagcaaaa tcgacacaaa
    17821 ttgtggtgaa ggaaatccgt ttcggaccac aaactgacga acatgattat gagtttaaaa
    17881 agaaaaatgc tgaaaaattc ttaaaagaag gtgctaaatt aaaagcattc gtattcttta
    17941 aaggacgttc aattatatat aaagaacaag gtcaaattct tttgcttcgt ttagctcaag
    18001 atttagaaga actgggaaaa gtagaggctt taccggtatt agaagggaag cgaatgatta
    18061 tgtatctcgc acctaagaaa aagaagtaag taagttgaat cacaaataaa tttgggaaat
    18121 tatgcctaaa atgaaaacaa aatccagtgc taagaagcga ttcaaagtaa ctggctctgg
    18181 aaaaatcaag agaaaacacg cttttaaaag tcacattttg actaaaaaat ctaaaaagcg
    18241 taaattagct ttaactcatt cgactttggt tcacaaaacc gatgagaaaa gtattaaaca
    18301 acaattaaga attatctaat cgacaatttc aaatgtcaat tacaaattaa ttctttaggt
    18361 taaaaaaatt ttaataacct tggagtatgg ccaataagtt cccttgatta aattcgggac
    18421 gcctactaca aaaaacaaga aaattatgcc aagatcagta aattctgttg ctaaaagagc
    18481 acgaagaaaa aaagtattaa agcaagccaa aggtttcttt ggtagacgta aaaacgtttg
    18541 gacggttgct aaaaacgcgg ttgaaaaagc catggtttat gcttaccgtg atagaaaaca
    18601 aaacaaaaga aacttccgtt ctttatggat catgcgtatt aacgctggtg ctcgtttgga
    18661 aggaatgagt tattctcaat ttatcggaaa agtaaaagct aacggtatcg aattgaaccg
    18721 taaagtgctt gctgatttag caatgaatca tccggaagct tttaaagcaa ttttgaataa
    18781 agtaaaataa acgttttaac aatccgattt taacttactt acatagaaaa tcccaatcga
    18841 aagtttggga tttttttata actattttat ttacatgaaa acctctacct tatctccgtc
    18901 aaaacttgca aagaccatac ttggatgcga atccttgtga aaaatagttc cttctttatc
    18961 gattgcaatt gctcctgcaa aaccatcaaa cggtttgagt tcattaaagg ttttttcaaa
    19021 agcagcttct aaagtgaatc catcggttac tcgggtaaca atttttgtag caacagctcc
    19081 acttacaata tcttctccta ctccggtcaa acttacacca caaaattcat tggcataatt
    19141 tccggcaacg gttgctgaat ccgaaattct gcccggaatc tcaaagcctt ttccgccagt
    19201 tgaagtagca acagctattt tgccatttga atctaaagca acacaaccca cagttcctat
    19261 tcccgtagct tgtaatttag cttcatactc tgctcttctt tgaggaattt ctgttgaaaa
    19321 aggttcaaat ccgtgctttc tggcaaaact ggttgctcct ttccctccca aaactctatc
    19381 gtcaacattc aacaaaactt ctgctacttg aattgggttt tttacttctt caatattgat
    19441 tacgccactc attttcaaag aagttccatc cattattgcg gcactcattc tgatttttcc
    19501 atcgctttga atttgtgaac caataccagc attgaacaat tcatcatctt ctaataatga
    19561 tacagcatag acaaccgttt ctaaagccga atgtgtagta aggtactcat aagaatcctt
    19621 aacaattcgc aataaagcct cttgctttgc aattttagtt tctagattag tggaagattc
    19681 tgagaaaaaa cctccgtgga taattatttt agccataact gtaaattaaa aagggacaca
    19741 gtttagagaa atttaaaatt tatataattt ctctaatctg tgttccaaaa atatttcaaa
    19801 attaaattca tacgtattgt gacgaatgaa tgtgcatttt gtaggtttct aaatcaaatg
    19861 tatcgtgcat actcatgaca tgcgtaacta aatgattaat tgaaattggc tgacctaatt
    19921 ctacctgtgt taaattagta tctttaattt ctcgcccatc aactaaaata actaatccag
    19981 aacctatggc ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtatcttt
    20101 aatttctcgc ccatcaacta aaataactaa tccagaacct atggcttcca ttttagaatt
    20161 gtgtgtaatc aaaagtccgg tgtcttctcc taagcctatt cctaacactt ttgggtttcc
    20221 aacaaccgct tgaaataaac gtccaatgcg acctctttgc acaaaatgtg tatcaataat
    20281 cacatcatca attaacccta aaccactggt gatttttact tctcctttta aaagtgcttc
    20341 atgactactt ccctgatata tcatattatt agaggctgct gctgctcctg ccgaagttcc
    20401 ggcataaata aaaagttcat ttttatattt atccaataaa atatcgtgaa actttgtgcc
    20461 tcccaaaatg gatgataatc gcaattggtc cccaccggta aacataacaa tgtcagctgc
    20521 ttttaaacgg tctatatttt cttgctcact agcttgttca cgacgttcaa tgtgcaaaac
    20581 atcaacgttg gttgcattta aatattgaaa tgctttggca tattccggac caacttctct
    20641 cggaatcttt gaagctgttg tgatgatttc aattcttgat ttttctttat attttgattc
    20701 gtctatgatt cgttttaaaa ttcctttttc aaagaaattc atgtttttag aaacatcctg
    20761 atcaaaactc gattcagtaa aactgccttt atcaacagct ccaccaatga taattaattt
    20821 tcctctgcta ttcattttgt aaaaatagta aaatctatat aggcagcaat atttttgagt
    20881 tattttaagg taaattaaac acgacaatca aactatagta aataacagaa aaatttactt
    20941 aatcccttat ttatgaagta aataataact cacttaaaaa ctcaatgtgt attttccata
    21001 tcaattcaaa aaattttggc ataatatgca aaaaaaatac agtagcaaag taaatatttc
    21061 ttatttttaa caaaatcatt cacaaaacaa aaactaattt ttccaatcaa tttcaagttt
    21121 tatgaaaata ttaaaagtac aagcacttcg cggtccaaat ttatggagta ttcaacgcaa
    21181 aaaattaatt caaatgcgtt tggatttaga agaaatggag caaaatccaa ccaataaaat
    21241 tgacggtttt agagaaagat tggaagctat gtttccggcc atgatagaac accgctgttc
    21301 agaaggtgtc cgaggtggtt tttttatgcg tattgaaaaa ggaacttgga tgggacatgt
    21361 tatcgaacac atagctttag aaattcaaac ccttgccgga atggaaaccg gttttggaag
    21421 aacaagagag accaaaaccc ctggagttta taatgtcgtt tttagttaca cggaagaaaa
    21481 cgtcggaatg tttgccgctg aaagttcagt aaggatagcc gaagctttaa ttgccggtac
    21541 ggattatgat ttagaagcgg acatcaaaaa aatgaaagaa attcgggaac gtgttcgtct
    21601 tggaccttct accggaagca ttgtcgaaga agccgtttcg agagatattc cttggattcg
    21661 attgggaaca aactccttag tacaattagg ttacggagtt aatcaaatgc gttttcaagc
    21721 tacaattact tgtaaaacta gtaacattgc tgttgatatt gcttgtaata aagaagaaac
    21781 caagagaatg ttagaattgg cttccattcc ggtggcaagt ggttcaattt gtgtagatga
    21841 agaagattta gaacttacta ttaaaaaaat tggttatcct attgttataa aaccattaga
    21901 tggtaatcat gggaaagggg cttccattaa tgtggtaact tgggaagatg ccaaagaagg
    21961 tttagaacac gccaaaaaat atagccgtcg agtaattgtt gaaaaattta ttaccggatt
    22021 tgattttcgg gttttagtca tagataataa attggttgct gccgccaaaa gagttcctgc
    22081 acacgtagtt ggcaatggaa aaaacactat tcaggagttg attgacatta ccaacctcga
    22141 cccaaaaaga ggttatggac atgaaaatgt attaactgaa atcactgtag accgagatac
    22201 aactgattta ttggaaaaaa tgggttacac attagaaacc ttaccaaata atggagaaac
    22261 ggtttattta aaatcaacag ctaatttaag caccggtgga acttcagtag atgtaacaga
    22321 tatgatgcat cctgaaaata tctttttagc tgaacgtatt tcacgtgtaa ttggtttaga
    22381 tgtgtgcgga attgatataa tggctgaaaa tttgacccaa cctttaaaag aaaatggcgg
    22441 agttatttta gaagtaaatg cagccccagg tttcagaatg catttagacc cctctgaagg
    22501 attggccaga aacgtagctg ctccggtaat tgacatgctt tatccaccag gaaaaccaag
    22561 tcgtattcca attattgccg ttaccggaac aaacggaaaa acaacgacta cccgattgtt
    22621 agcccatatt gttaaaaata atggcttccg agtaggtttc accacttcag atggaattta
    22681 tattcaaaat cacatgttgg aaaaaggtga cacaaccgga cctttgagtg ccgaatatat
    22741 tttgaaagat ccaaccgtag aatttgccgt tttagaaact gctcgaggtg gaattttaag
    22801 agctggttta ggctttagcc gatgtgacat tgcaattata acaaacattc gcgaagacca
    22861 tttgggatta aatgacattg atacgctaga cgatttggct cgtgtgaaaa gtgtagttgt
    22921 tcgaagtgtg aaaaaagacg gttgggccat tttaaatgcc gaagacgatc agtgcatgaa
    22981 aattgccaac gatttgaatt gcaacattgc ttatttctct atggatgaag aaaatccggt
    23041 agctaaaaaa ttaagtaaag aaggtaaaat tgttgctgtt tatgaaaatg gttttgtgac
    23101 tatcaaaaaa ggagattgga aaattagaat tgaaaaagta ttacatattc cattgacttt
    23161 aggcggaaaa gcaaaattca tgattcaaaa tgttttagca gccacactag ctagttattt
    23221 atatggtttt aaaacagatg acattagttt atctttacaa acttttattc cgagtgtagc
    23281 tcaaactcct ggtcgaatga acatttttga atttaaacga ttcaaagtca tgattgattt
    23341 tgctcacaat ccggccggat attctgccat tgaagatttg ttgagtaaca ttgatgctac
    23401 ccgaaaaatc ggaatcattg ccggtgttgg cgacagaaga gatgaagata ttcgagattg
    23461 tgctgaaatt gcggcccgaa tgtttgacca tatcattatc cgacaagaaa aacatttaag
    23521 aggtcgaact gaacaagaaa ttattgattt gattttagaa ggaattcaat tggcaaatag
    23581 agaagttacc tacgaaatta ttccaaaaga aattgaagcg atcaaacatg ccatcaatat
    23641 tgcagatgaa ggcactttta tcactgcctt aagtgatgtg gttaccaacg ctattggtat
    23701 tgttcaggaa tatttagata aggaaaatga agagaactt
//
LOCUS       DAOTCW010000019        23671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1113_length_23671_cov_41.611196, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000019 DAOTCW010000000
VERSION     DAOTCW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 23671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23671
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1113_length_23671_cov_41.611196"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..551
                     /locus_tag="P5335_02765"
     CDS             126..551
                     /locus_tag="P5335_02765"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HRZ73836.1"
                     /translation="MTTQNEKTTAALLHVSAFAKYLIPFAGIVVPLIIWQTKKHQSEY
                     VDANGKSVINFHLSILVYSIVLAIILGIFFLNSIIQVVQIESAGGEVFPVELISTVII
                     GAIVLGAWTITEFILIILGSVKANEGTVYKYPFTINFIK"
     gene            615..944
                     /locus_tag="P5335_02770"
     CDS             615..944
                     /locus_tag="P5335_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRZ73837.1"
                     /translation="MNIENTKAQMRKGVLEFCILSVLKEKEAYTSEILDTLKNAKLLV
                     VEGTVYPLLTRLKNDGLLNYRWEESTSGPPRKYYGLTEIGKQFLTELNSTWGELSSAV
                     NTITNQN"
     gene            950..2683
                     /locus_tag="P5335_02775"
     CDS             950..2683
                     /locus_tag="P5335_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRZ73838.1"
                     /translation="MNKTVNINLGGLFFHIDEDAFQKLNRYFDAIKRSLSNSSGQEEI
                     MKDIEMRISEIFSQNKETDKHVINLKDVDSMIAVMGQPEDYRIEEEGEPAQKNFNNSS
                     KTSKKLYRDIDKNLLGGVSAGLGHYFGIDALWIRLAFVLIVIAGVGFPIFLYILLWVL
                     IPKAQTTTEKLEMTGEPVNLSNIEKKVREEFAAFSDKVENADYEKMGNQAKSGAEKVG
                     SAIGEIFMTIFSIFAKFIGAILLIFSIFVLGGILIGGFTFGSTTFVDMPWQSYYDAVI
                     VSEFPIWLVVLLSILAVGIPFFFLLILGLKLLITNMRSIGNVAKYTLLALWLIAVGIL
                     ISFGIKQATEVAYSGKVVQKEVLAFNTSDTLDIQFKFNDYYAKEFRNNDFEVTQDENG
                     NEIIYSNDISIEFMYTDEAQPYLQIERRSEGNSMNEAKKRAEKINYGFKFEGNKLILD
                     NYFVTDLKSKYRNQEVELFLYLPKGTIVRPDTSVQDYDESNNTFFNLHYSADNYIYKV
                     EQQKIFCLNCPIEENEYNDISNDTIPNTSSTSVTIDKNGIQIKEENKAEEKRDFKGLK
                     INEDGIIIKTN"
     gene            2686..3426
                     /locus_tag="P5335_02780"
     CDS             2686..3426
                     /locus_tag="P5335_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRZ73839.1"
                     /translation="MVKLIVYITKTIVAILVALVVASCQYSIDLGDSIKGSGNVISEN
                     RPVNGDFTSIEVSRGIDVIVAQSNIKSIEVKADDNIIKHISTDINNSVLTITLDKSVK
                     NLSSKKVYVSLPNIESLRSTSGSSISSKNTLVVKSIDVKSSSGSEINIDVEAEKIICE
                     SSSGSEISAEGKAIKLEVSSSSGSEIEAQNLLANDIIANSSSGSSIEIHPIISLSAKA
                     SSGSDIIYYNNPKSLTKKSSSGGSIKSN"
     gene            3529..4365
                     /locus_tag="P5335_02785"
     CDS             3529..4365
                     /locus_tag="P5335_02785"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRZ73840.1"
                     /translation="MKNTIILLTVILFSTVAWSQKKEKIKASKIVTVSQKEIGNFDSL
                     EILDNIDVFLVKGNSCAIEIEADDNTHEIIDFKLSGSTLRIGLTHDITGAKKTSVRVT
                     YTDSFKMLVAKGEADVTALAEVKLDDITFKCYDESRLFLNANCKNFTLIANDKSKVEI
                     NLKSEDTVLELSKNAYVKGLISTLKLKCDMYQKSIAIIEGDADDFKLRMDNYSNFTGK
                     NLTSKTASLIVEANSKNSILATTKLVLEASGKSETHFYGEGKVELKKFDEEATLFKKL
                     LK"
     gene            complement(4457..4531)
                     /locus_tag="P5335_02790"
     tRNA            complement(4457..4531)
                     /locus_tag="P5335_02790"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4494..4496),aa:Pro,seq:tgg)
     gene            4732..5679
                     /gene="trxB"
                     /locus_tag="P5335_02795"
     CDS             4732..5679
                     /gene="trxB"
                     /locus_tag="P5335_02795"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRZ73841.1"
                     /translation="MSNTIEKVKCLIIGSGPAGYTAAIYAARASMQPVLYQGTQPGGQ
                     LTTTNEVENFPGYPDGVTGPEMMIQLQAQAKRFNTDIRDGWATKVDFTGPIHKVWIND
                     TIELHCETVIISTGASAKYLGLPSEQHYLKLGGGVSACAVCDGFFYRNQEVVIVGAGD
                     SACEEAHYLSKMCKKVTMLVRSEKFRASKIMEARVRKTENIEILLNTETIEVKGDGQV
                     VSSVLVKERTSGVETEIPATGFFVAIGHEPNTAIFKDFITLDETGYIKNIPGTSKTNV
                     PGVFVAGDAADHVYRQAITAAGTGCMAALDAERYLASKE"
     gene            5921..6529
                     /gene="udk"
                     /locus_tag="P5335_02800"
     CDS             5921..6529
                     /gene="udk"
                     /locus_tag="P5335_02800"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRZ73842.1"
                     /translation="MLIIGIAGGTGSGKTTVVHQIMNELPDTEVGVISQDSYYKETHN
                     LSYEERTKINFDHPRAIDFELLVNHLKELKAGNIIEQPVYSFVTHNRTDDTIITHPRK
                     VIIVEGILILSHPELRDMFDIKVYVHADSDERLIRRLKRDIAERGRDMNEVINRYQTT
                     LKPMHEQFIEPTKAFADIIIPNDKYNTVAIDVVRAVINQRIL"
     gene            6532..6879
                     /locus_tag="P5335_02805"
     CDS             6532..6879
                     /locus_tag="P5335_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRZ73843.1"
                     /translation="MNFIQRYKEKHPWFKYVGNRYVLVLVFFAVWMLFLDNYSYIEHR
                     VLNKELDELETNKKYYQDEIKKDCLDIKRLNNPDQIEKYAREKYYMKRDSEDIYIIEF
                     EEDLLEEENNKSL"
     gene            6883..8238
                     /locus_tag="P5335_02810"
     CDS             6883..8238
                     /locus_tag="P5335_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HRZ73844.1"
                     /translation="MSKHLFNEFEPVSSKQWKQQIQYELKGADYNETLVWESPEGIKV
                     KPFYHADENQLKFESPISNGFKIIQNIYVYDISKSNERAAESINRGAESIRFTLESDA
                     VSLEKLMDNLPKENVIYYFNVLFHSSDFIKKAASFASQNNYKIFVLQDAIGQLAKDGN
                     WFESLEKDCNQLQSVALLNFPFLSCKIDLYQNAGANIIQQLAYAMAHVNEYFNRIEGI
                     STPITFEVSVGTNYFFEIAKLRALRLLFDSLAKEYDYTFDCHIIVTPTKRNKTLFDYN
                     VNMLRTTTECMSAILGGADGISNLAYDALYHKDNEFGDRISRNQLLVLKHESYFNQVH
                     NPADGAYYIESLTEQLAEKALELFKDIEANGGLITQLIEGTIQRKINESAQKEQDLFD
                     SGKEVLLGTNKYPNKNDKMAKELELFPFVKTNARKTLITPIIEKRLAEKTEQERLSLE
                     A"
     gene            8279..>8562
                     /locus_tag="P5335_02815"
     CDS             8279..>8562
                     /locus_tag="P5335_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRZ73845.1"
                     /translation="MKRKNIQQLTLKSAVKKADTSSVVPFSTAEHIELKQSYHKSDVE
                     KLEHLGFAAGFAPNLRGPYATMYVRRPWTVRQYAGFSTAEESNAFYRRNLA"
     assembly_gap    8563..8662
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8663..10560
                     /gene="scpA"
                     /locus_tag="P5335_02820"
     CDS             <8663..10560
                     /gene="scpA"
                     /locus_tag="P5335_02820"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRZ73846.1"
                     /translation="AGFSTAEESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHERVV
                     GDVGKAGVAIDSVEDMKVLFDQIPLGEMSVSMTMNGAVLPIMAFYIVAAEEQGVAPNL
                     LSGTIQNDILKEFMVRNTYIYPPTPSMKIIADIFEYTSKNMPKFNSISISGYHMQEAG
                     ATADIELAYTLADGLEYIRTGLAAGMDIDTFAPRLSFFWAIGMNHFMEIAKMRAGRML
                     WAKLLKQFNPKDEKSLALRTHCQTSGWSLTEQDPFNNVARTCIEAAAAAFGGTQSLHT
                     NALDEAIALPTDFSARIARNTQIFLQEETKICKTVDPWAGSYYVESLTNEIAEKAWAL
                     IEEVEELGGMTKAIEAGIPKLRIEEASARKQARIDSGQDIIVGVNKYRLEKEDPLMIL
                     DVDNQMVRKQQIESLNQIKASRNNEQVKICLAKLTEAAKTGTENLLTLAVEAARNRAT
                     LGEISDALEEVFSRYKAQIRTFSGVYSKEIKNDVSFEKAKQLADAFAKKEGRRPRIMI
                     AKMGQDGHDRGAKVVATGYADVGFDVDIGPLFQTPAEAAKQAVENDVHILGVSSLAAG
                     HKTLVPQVIEELKKYGREDIMVIVGGVIPHQDYQFLFDAGAVAVFGPGTKISEAAISI
                     LEVLLED"
     gene            10633..11196
                     /locus_tag="P5335_02825"
     CDS             10633..11196
                     /locus_tag="P5335_02825"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRZ73847.1"
                     /translation="MLTLSFTPFPILESNRLLLRAVSTDDVNEILELRGNPETMKFIP
                     RPLVTSTEMALEHIGMITEKIENNIGINWAITVKGNPKLLGVIGHYRIQTENYRSEIG
                     YMLLPEYQGQGIVSEAINMVLEYGFNVLNFHSVEAVVDPENHVSAKVLEKNGFVKEAH
                     FLENEYWDGKFWDSAVYSLLKRNFVKK"
     gene            11292..>11577
                     /locus_tag="P5335_02830"
     CDS             11292..>11577
                     /locus_tag="P5335_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRZ73848.1"
                     /translation="MAIDFNAINLSQGFPNFPIDEKMAEIIRRLAGSDVHQYTPMAGY
                     PSLMEKIAKLTAQSYQRAINPLTELLITAGATEAIFATIQALVHSGDEVLI"
     assembly_gap    11578..11677
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11678..12526
                     /locus_tag="P5335_02835"
     CDS             <11678..12526
                     /locus_tag="P5335_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRZ73849.1"
                     /translation="AIFATIQALVHSGDEVLILDPSYDCYETPILLSNAKPVRVSLNE
                     DYSPNFEKIAHHLSSKTKLLILNNPHNPSGKMWCEKEFVELENLLEKFPDVLLLSDEV
                     YEYIALENNHISVNTREKLRHRAIITSSFGKTLHLTGWKIGYAVAPEHLMKEVKKVHQ
                     FLVFSVNSLSQAAISDYLDIFDISSVKTVYQQKSNFFQNLMKETSFELLPCEGTYFQV
                     ASYKGISTESDLDFTKRLVTEFGVSTIPLSIFYGDNTDLKRIRFCFAKDDETLTKAAE
                     RLLKLK"
     gene            12599..13480
                     /locus_tag="P5335_02840"
     CDS             12599..13480
                     /locus_tag="P5335_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRZ73850.1"
                     /translation="MNFSAKMLRDNALEGKVIVVTGGGSGLGKAMTKYFLELGAKVAI
                     TSRDLDKLTTTAAELESQTGGICLPLQCDVRHYDQVEAMLQEVLKTFGKADVLMNNAA
                     GNFISPTERLSANAFDTIIDIVLKGSKNCTLAFGKHWIDTKQESSVILNIVTTYAWTG
                     SAYVVPSATAKAGVLAMTRSLAVEWAKYGIRSNAIAPGPFPTKGAWDRLLPGDLAEKF
                     DMAKKVPLKRVGDHQELANLAAYLVSDFSAYVNGEVITIDGGEWLKGAGQFNLLEDIP
                     EEMWDMLEAMIKAKKNG"
     gene            13620..14384
                     /locus_tag="P5335_02845"
     CDS             13620..14384
                     /locus_tag="P5335_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRZ73851.1"
                     /translation="MGKIIAIANQKGGVGKTTTTVNLAASLGVLEKKVLLIDADPQAN
                     ASSGLGIDVDSVAIGSYQVLEHSNTAAEAIMKCSAPNVDIIPAHIDLVAIEIELVDKE
                     NREYMLKEALASIKDQYDYILIDCAPSLGLLTLNALTAADSVIIPIQCEYFALEGLGK
                     LLNTIKSIQRLHNPDLDIEGLLLTMFDSRLRLSNQVVEEVQKHFNDMVFKTIIQRNVK
                     LSEAPSFGESIINFDATSKGANNYLSLAEEIIKKNS"
     gene            14391..15293
                     /locus_tag="P5335_02850"
     CDS             14391..15293
                     /locus_tag="P5335_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRZ73852.1"
                     /translation="MTQAIKKQALGRGLSALLKDPENDIKSVNDKNADKVVGNIIELE
                     LEAIEINPFQPRSNFNEETLQELASSIKELGVIQPITVRKLEFNKYQLISGERRLRAA
                     KIAGLTVIPSYIRIADDNESLVMALVENIQRHDLDPIEIALSYQRLIDEIQLTQEQMS
                     DKVGKKRSTIANYLRLLKLDPIIQTGIRDGFISMGHGRALITIENEDIQSDIYHKIIS
                     QNLSVRETENVVKNYQESLKPKSGKTIKANPSFSVSEMDKKEIGTTLGTKVDIKIDAK
                     GKGKITIPFKSESDFQRIIALIKG"
     gene            15293..15856
                     /locus_tag="P5335_02855"
     CDS             15293..15856
                     /locus_tag="P5335_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HRZ73853.1"
                     /translation="MHSLRIIFFAFSLLGTTFANAQVQIEEFGQTQDTLKYKPIDPLA
                     PSKAAFYSAILPGLGQAYNKKYWKIPIVYGGLAIGYYFYNDNNKYYNEFRDIYKRRLE
                     GYTDDKYYGIYSDNVLINAQRTYQRNRDISLMVMIGIYVLNIIDANVDAHLIQFNVNE
                     NLSLRPDININDITRKQNLGLVLNYKF"
     gene            15856..16557
                     /gene="dapB"
                     /locus_tag="P5335_02860"
     CDS             15856..16557
                     /gene="dapB"
                     /locus_tag="P5335_02860"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRZ73854.1"
                     /translation="MKIALLGYGKMGKVIERIALERGHEIVLRKSSSDSFEGLELADV
                     AIDFSIPNAAVSNISYCIEQGIPVVSGTTGWLENYHTMVQLCQSKKAAFIYGSNFSLG
                     VNLFFELNDYLAKMMAKFNQYNVSMEEIHHTQKLDKPSGTAISLANSIINHTDKNSWS
                     IENPLKDDLFIDVKRVDNVPGTHTIFYTSAVDTIEIKHTAHNRDGFAFGAIIAAEWIV
                     GKTGVFTMKDVLGLK"
     gene            16649..>17626
                     /gene="lepB"
                     /locus_tag="P5335_02865"
     CDS             16649..>17626
                     /gene="lepB"
                     /locus_tag="P5335_02865"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRZ73855.1"
                     /translation="MTLYQWFIFFLVIQLVHYICTWKLYQKAGRQSWEAAIPVYNAVI
                     LMKIINRPWWWTFLLFIPIVNLIMFPVIWVETLRSFGKHSSLDTLLAVITFGLYIGYV
                     NYTQEVTHIKERSLHPKNPTADFVSSILFAIVVATTVHTYVMQPYTIPTSSLEKSLLI
                     GDFLFVSKFHYGARTPTTAIALPMVHDSIPFVKTKSYFSWPQLPYFRFPGIQSVERND
                     IVVFNWPKDTVYKFFDRSGKRADKPLDKKSNYVKRCTAIAGDSLQIIKGDVFVNGKLL
                     PLHDRQRLQFSYNVETDGSQFNEEYIFKELQITDGIYVNSQNSYTFSALT"
     assembly_gap    17627..17726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17727..17942
                     /locus_tag="P5335_02870"
     CDS             <17727..17942
                     /locus_tag="P5335_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRZ73856.1"
                     /translation="DHIVGKPVFIWMSLDQNEPWGKLFDKVRWERMFTTVGGSGKPVS
                     YFIYFVIALVAWFGFDYFRKKRKKSAE"
     gene            17951..18562
                     /locus_tag="P5335_02875"
     CDS             17951..18562
                     /locus_tag="P5335_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HRZ73857.1"
                     /translation="MKALLYPTYFPSISHYAAMVQADSITFETEDNFQKQTNRNRMYI
                     YSPNGIQLLNIPIKHSKEAHQKTKEVKIEQDFNWQKQHFKSLEAAYRTSPFFEYFEDD
                     ILPVFKKKQTFLLDLNLEVFEIVNQCLGITLPFEKTAEYFQNSSDYIDFRSLANGKKD
                     QTSCERYTQVFEEKHGFLNNLSILDLLFNEGRYALDYLKNQKL"
     gene            complement(18563..19618)
                     /locus_tag="P5335_02880"
     CDS             complement(18563..19618)
                     /locus_tag="P5335_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRZ73858.1"
                     /translation="MKNLNWFNKVMFFFNIVLTITTFIAYVLPFLAPKLFPILSVLTL
                     VLPLMLIFNLLFFVYWLLQFKKQMLLSGIVLLLGITFVTKFYKFSETNLPIEDEDFVL
                     MSYNVRLFNLYKWIERDDVSSQVSSFIKEQNPDILCLQEYSRNNDVDFRIYKHKYIFV
                     EGKNTKLGQAIFSKYPIIEEGIIEFPNSTNNAVYADIKRGKDTIRVYSIHLQSIKITP
                     DVHEIDENIQGVSQDQSKRIFRRMSASFKTQQQQAEIIKKHKLDCKHPVIICGDLNNS
                     AYSFVYRSIKGDLNDTFEEAGSGFGKSYNFKYYPGRIDYVFTDKLFLVKQFTTHSEFV
                     NSDHFPIVTRLAFKKIE"
     gene            complement(19661..20533)
                     /locus_tag="P5335_02885"
     CDS             complement(19661..20533)
                     /locus_tag="P5335_02885"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRZ73859.1"
                     /translation="MSILKELKQHYILGGVTEKLIYWNVALFAIPWIIKGLLSLFGIG
                     FNFTDYISLSSNPSDLAWKPWSIISYAFFHADIFHILFNLIILNFVGRLFLTFFTQKQ
                     LLSLYLMGAIFAGLIYILSYLFFPALSNQLVTLVGASGAIMAVLFAVSSYAPHMQIRL
                     LLIGNIKLWHMAVFYLAIDLISLSYTNVGGHIAHLGGALFGYLYASQLKKGTDITKGF
                     TSLMNSIVNLFKAKKNTPFKKVHKNTIYTKPKPSPKDKSQQQIDEILDKISKSGYDSL
                     TKDEKEFLFKAGKS"
     gene            complement(20582..>21219)
                     /locus_tag="P5335_02890"
     CDS             complement(20582..>21219)
                     /locus_tag="P5335_02890"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRZ73860.1"
                     /translation="PNLMHIFFNMFGLIMFGSALEHFWGWKKFLFFYISCGLGAALLH
                     TGMNYYEINSLLQQVSELNISASDMHILMNIDHTNQFDVNNKIIDGSLNNILEKVNAN
                     QEQYNIIVRAAIKYQTAAVGASGALYGVIVAFAFMFPNAELMLLFLPIPIKAKYFVPV
                     LVLGDLFMGLKGESIFGGGGIAHFAHVGGALTGFLMMWFWKKNQFNEKRWD"
     assembly_gap    21220..21319
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21320..22746)
                     /gene="mutL"
                     /locus_tag="P5335_02895"
     CDS             complement(<21320..22746)
                     /gene="mutL"
                     /locus_tag="P5335_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRZ73861.1"
                     /translation="MSSIIQLLPDHVANQIAAGEVVQRPASVVKELLENAVDAKATDI
                     KLIVKDAGKSLVQVIDNGNGMSVTDARMCFERHATSKIRHAEDLFTIHTKGFRGEALA
                     SIAAIAHVELKTKQEFDELGTHIIIEGSKFITQDPSVLPKGTSFAVKNLFFNIPARRN
                     FLKSETVEHRHIVDEFQRVALAHPSIHFTFIHNGSELFNLHSSNLRQRIVAIFSGKTN
                     EKLVPISEETEIVQVNGFISKPEFSKKNRSEQFFFVNNRYIKSGYLHHAVMAAYEGLL
                     KDGMQPGYFIYLEVPPNTIDINIHPTKTEIKFDDEHALYAILRSTVKHSLGQFNVAPV
                     LDFERDPNLDTPYHYQNKDASTPLIQVNSNFNPFAEEKATMNFGGSTYRERKTENAHW
                     ESLYVGLKNDTKEIESFSFEQEAVSSSLFNDTEIEESKNRTYQIQKKYILSSIKSGMM
                     VIDQKRAHQRILYEQYLKNMTVQQAS"
     gene            complement(22746..23039)
                     /locus_tag="P5335_02900"
     CDS             complement(22746..23039)
                     /locus_tag="P5335_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73862.1"
                     /translation="MIKKSLFKLPKNRSFNYTPRHYEGKELGNLYDFDSKFNKNRETV
                     NYNDFRAHWSDARNDSRHRGNREINFRLIAIILVLLLIFLFIIDFDLSIFIKR"
     gene            complement(23164..>23671)
                     /locus_tag="P5335_02905"
     CDS             complement(23164..>23671)
                     /locus_tag="P5335_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HRZ73863.1"
                     /translation="KTRTIQVMDSEIAVIEDIIVNDLIDENSIVIVVSGIGNYVYSID
                     GVVFQESTIFTNIEPGLITIYVKDLYNCGIETENINVLSIPKFFTPNSDGYNDLWNLR
                     GITQDSNIKVKIDIFDRYGKLLFQITPINQGWNGTINGNPLPADDYWYVISLDDNRTI
                     KGHFSLKR"
BASE COUNT         8268 a         3750 c         3897 g         7356 t
ORIGIN      
        1 aaaaatagaa ttaatttatg aaatatcaat ttgttaaaaa attgttaaat attagtacta
       61 tgtgtaacac aataccttct tttagacata tatttgcata ggatattatt atatactttt
      121 ttattatgac aacacaaaac gaaaaaacaa cagctgcact actacatgta agtgcatttg
      181 caaagtatct tattccattt gccggaatag ttgttccact gattatatgg caaaccaaaa
      241 aacatcaatc tgaatatgtt gatgccaatg gtaaatcagt tattaatttc catctaagta
      301 tattagtgta tagtatcgtt ttggcaatta ttctaggcat cttttttttg aattcaatta
      361 tccaagtcgt tcaaattgaa agtgcaggag gagaagtttt tcctgtcgaa ttaatttcaa
      421 cagttattat tggagcaata gtattaggag catggaccat cacagaattc attctaatca
      481 tacttggttc tgtaaaagca aatgaaggaa ccgtttacaa atatcccttt acaataaatt
      541 ttatcaaata aaagtttcaa tcatcaaaat caatcaaaaa acgaattgtt aatcaaaaaa
      601 tcacaacatg aattatgaat attgaaaaca caaaagctca gatgcgaaaa ggtgttttag
      661 agttttgtat actttctgtc ttgaaagaaa aagaagctta tacctctgaa attttagaca
      721 ccttaaaaaa cgcaaaatta ctcgtggttg agggaacagt ttatcctctt ttaacccgat
      781 tgaaaaacga cggattactc aactatcgtt gggaagaatc aacttcggga cctccaagaa
      841 aatattatgg attaaccgaa ataggaaaac aatttttaac agaactaaac agcacttggg
      901 gcgaattatc ctcagctgtt aacaccataa ccaatcaaaa ctaatagtca tgaacaaaac
      961 agtaaatata aatttaggag gtctattctt tcacatcgat gaagacgcct ttcaaaaatt
     1021 aaatcgctat ttcgatgcta ttaagcgttc tctttcaaat tcatctgggc aggaagaaat
     1081 tatgaaagat atcgaaatgc gtatttcaga aatcttttct caaaacaaag agactgacaa
     1141 acatgtcatc aacttaaaag atgtagattc catgatagcc gttatgggac aaccggagga
     1201 ttacagaatt gaagaagaag gagaaccagc tcaaaaaaac ttcaataatt catccaaaac
     1261 atcaaaaaaa ttgtatcgcg atatagacaa aaacttacta ggaggagttt ctgccggatt
     1321 gggacattac tttggaattg atgctttatg gattcggtta gctttcgtat tgattgtaat
     1381 agccggagtt gggtttccaa tttttcttta tattttactt tgggtattaa ttccgaaagc
     1441 acaaaccact acagaaaagt tagaaatgac aggtgagcct gtaaacttga gtaatatcga
     1501 aaaaaaagtt cgtgaagaat ttgcagcctt ttcagataaa gtagaaaacg ctgattatga
     1561 aaaaatgggt aatcaagcta aatccggagc tgaaaaagtt ggttctgcaa ttggcgaaat
     1621 attcatgact atattcagca tttttgcgaa gtttattggt gccatcctat tgattttttc
     1681 catttttgtt ttgggaggaa ttctgattgg aggtttcacc tttggttcta caacttttgt
     1741 tgatatgcct tggcaaagtt attatgatgc tgtcattgtg agcgaatttc ctatttggtt
     1801 agtagttctt ttaagcattt tagcagtagg tattccgttt ttcttccttt taatattagg
     1861 attgaaatta ttgataacaa acatgagatc tattggcaat gttgcaaaat acactttgtt
     1921 agctttgtgg ttaattgctg ttggaatttt aatttctttt ggaattaaac aagcaactga
     1981 agtagcttat agtggaaaag ttgttcaaaa agaagtgtta gcattcaata cgtctgatac
     2041 tttagacatc caatttaaat ttaatgatta ctatgctaaa gaattcagaa acaatgattt
     2101 tgaagtcaca caagatgaaa atggtaacga aatcatttat tccaacgata tcagtataga
     2161 atttatgtac actgatgaag ctcagcctta tttacaaata gagcgacgtt ctgaaggaaa
     2221 ctcaatgaat gaagctaaaa aaagagcaga aaaaatcaat tacggattta aatttgaggg
     2281 aaataaatta attctggaca attattttgt tactgattta aaaagtaaat accgaaacca
     2341 agaagtagaa ttgtttttat atttaccaaa aggtacaata gtaagacctg acactagtgt
     2401 acaagattat gatgaatcta acaatacctt tttcaattta cattatagtg ccgacaatta
     2461 tatctataag gttgaacaac aaaaaatatt ttgtttaaat tgtcctattg aggaaaatga
     2521 atataatgat atctcgaatg atacaattcc aaatacttct tctacttctg taacaattga
     2581 caaaaacgga atacaaatta aggaagaaaa taaagcggaa gaaaagcgag atttcaaagg
     2641 cttaaaaatt aatgaagacg gtataatcat aaaaactaat taatcatggt taaattaatc
     2701 gtatacatca caaaaactat tgtagcaatt ttggttgcct tagtagttgc ttcctgccaa
     2761 tattctatcg atttaggaga tagcatcaaa ggaagtggaa atgtaatttc agaaaaccga
     2821 cctgtaaatg gtgactttac aagcattgaa gtgagtagag gaatcgatgt catcgtagct
     2881 caaagcaaca taaaatcaat tgaagttaaa gcagatgata atataattaa acacatctca
     2941 actgatatta ataattccgt tttaactatt acgttagaca aaagtgtaaa aaatttaagt
     3001 tcaaaaaaag tgtatgtttc attacctaac atcgaatccc tcagatcaac gagcggtagt
     3061 tcgataagca gtaaaaacac attggtagta aaatcgatag atgtgaaatc aagtagtggc
     3121 agtgaaatca atatagatgt tgaagctgaa aaaatcatct gcgaaagcag tagtggcagt
     3181 gaaattagtg cagaaggtaa agctataaaa ttagaagtat cttcttcgag cggaagtgaa
     3241 attgaagctc aaaatttatt agcaaatgat attattgcaa attcgtctag tggaagttct
     3301 attgaaatac atccaataat aagtttgagt gctaaagctt caagtggaag tgatattatt
     3361 tattataaca atccaaagtc attgacaaaa aaatcttcct caggtggctc aataaaatca
     3421 aattgattca ttgcgacttt aatttgatag gtaaatccat atcttaacaa acatcccgcc
     3481 ttaaaatcgg gatgtttttt tatatttgtt ccataaacaa tttcgaaaat gaaaaatacc
     3541 attatcctac tcactgttat tttattttcc actgttgcat ggagtcagaa aaaagaaaaa
     3601 ataaaagcct ctaaaattgt cactgtttca caaaaggaaa ttggcaattt tgacagttta
     3661 gaaattcttg acaatattga tgttttttta gtaaaaggaa attcatgtgc aattgaaatt
     3721 gaagccgacg ataatacaca tgaaattatt gatttcaaat taagtggtag cactttaaga
     3781 attgggttga ctcatgatat cacaggtgca aaaaaaacaa gtgtccgagt aacgtatact
     3841 gatagtttta aaatgttagt tgccaaaggc gaagctgacg taacagcact tgcagaagta
     3901 aaactagatg atataacctt taaatgttat gatgaatcgc gtttgttttt aaatgcaaac
     3961 tgtaaaaatt tcactttaat tgctaacgac aaatctaaag ttgaaattaa tttaaaatct
     4021 gaagataccg ttttagaatt aagtaaaaac gcctatgtta agggactaat cagtacgctt
     4081 aaactaaaat gtgatatgta tcagaaatca atcgcaataa ttgaaggcga tgcagatgat
     4141 tttaaattaa gaatggataa ctattccaat tttacaggta aaaatctaac ttctaaaacg
     4201 gcttctttaa ttgttgaagc taattctaaa aacagtattc tagcaacaac taaacttgtt
     4261 ttagaagcct ccggcaaatc agaaactcat ttttatggcg aaggaaaagt tgaattgaaa
     4321 aaatttgatg aagaagctac tttattcaaa aaattattga aataagaccg actttttttt
     4381 ggttaaacaa attaaagaac tgcctaaata actaaaaaaa ataaatcctt tccaattgga
     4441 aaggatttat ttttagtcgg ggtggcagga ttcgaacctg cggcctcctg ctcccaaagc
     4501 aggcgcgata accgggctac gctacacccc gaaaagcaaa tttcaaaatc gggatgtttt
     4561 caaatcattt cccttatttg aggttgcaaa tataaaacaa ataaaatgtt ttacaaatca
     4621 aaactcaaaa gaaaatttca ttttttttat ttattaaaac taatcaacaa ttaagaatta
     4681 tttttctatt aaaacttacc tttgtacttc gaaattgaaa aaaattaagt catgtcaaat
     4741 acgatagaaa aagtaaaatg tttaattata ggttctggcc ctgcaggata tactgctgct
     4801 atttatgccg ccagagcaag tatgcaaccc gtattatatc aaggaactca acccggtggt
     4861 caattaacaa ccactaatga agttgaaaat tttccgggtt atcctgatgg agttaccggt
     4921 ccggaaatga tgattcaact tcaggctcaa gccaaaagat tcaataccga cattagagat
     4981 ggttgggcaa caaaagttga ttttaccgga cctattcata aagtttggat taatgatact
     5041 atagaattac attgtgaaac ggtcattatt tcgacaggag catcagcaaa atatttaggt
     5101 ttaccttcag aacaacatta cttgaaatta gggggtggtg tttctgcttg tgccgtatgt
     5161 gatggtttct tctaccgaaa tcaagaagtt gtgattgtag gtgctggtga ttcagcgtgc
     5221 gaagaggcac attacctttc caaaatgtgt aaaaaagtaa ccatgttagt gcgaagtgaa
     5281 aagttcagag cttctaaaat catggaagcc agagttagaa aaactgaaaa tattgaaatt
     5341 ttattaaata ccgaaaccat agaagtaaag ggagatggtc aagtggttag ttcagtttta
     5401 gtaaaagaaa gaacaagtgg cgttgaaact gaaattccgg ctaccggatt ctttgttgcc
     5461 attggacatg aacctaatac tgcaatcttt aaagatttta ttacattaga tgaaacagga
     5521 tatatcaaaa atattcccgg tacatctaaa actaatgtcc ccggagtttt tgtggccggt
     5581 gatgccgctg atcatgttta ccgacaagca ataactgcag ccggtacagg ttgtatggca
     5641 gctttggatg cagaaagata tttagcgtca aaagaataat tacaccaaac aaatgaataa
     5701 aattatcccg acttgttcgg gattttttat ttgtaaattt tcaattgaga atgaatttat
     5761 atccttccat ttcacatttc ttaaaattca atcctgtttt ttaaatattc tgatttttta
     5821 aaacgttttc gttagtaact cctttaaaaa agatttgcat tactaattct aagtagttaa
     5881 attttcctaa atttgtaggg aataaaacaa taaataaatt atgctgatta ttggaattgc
     5941 aggaggtacc gggagtggaa aaacaactgt tgttcatcaa atcatgaatg aacttcctga
     6001 caccgaggtt ggagtaattt ctcaagactc ctattacaaa gaaacccata atttaagcta
     6061 cgaagaacga acaaaaatta attttgatca tcccagagcc atcgatttcg aattattagt
     6121 taaccattta aaagaactta aagcgggtaa tataattgaa caaccggtct attcttttgt
     6181 cacgcataat cgaactgatg acactattat tactcatccc agaaaagtaa ttattgttga
     6241 aggtatttta attctgtctc atccggaact tcgtgacatg tttgacatca aagtgtatgt
     6301 tcatgccgat tctgatgaac gattaattcg aagattaaaa cgtgatattg cagaacgggg
     6361 acgtgacatg aatgaggtta taaatcgcta tcaaaccact ttaaaaccaa tgcacgaaca
     6421 gttcattgaa ccaaccaaag cctttgcaga tattataatt ccgaacgaca aatacaatac
     6481 agttgccatc gatgtagttc gagctgtaat taaccaacga atattataaa catgaacttt
     6541 atccaacgct ataaggaaaa acacccatgg ttcaaatatg tcggaaaccg atatgtatta
     6601 gtacttgttt ttttcgctgt ttggatgctt ttccttgata actattctta tattgaacat
     6661 cgcgttttaa ataaagaatt ggatgaactt gaaaccaata aaaaatacta ccaagacgaa
     6721 attaaaaaag actgtcttga tatcaaacga ttaaacaatc ctgatcaaat tgaaaaatat
     6781 gctcgcgaaa agtattacat gaaacgcgac agcgaagata tttatatcat cgaatttgaa
     6841 gaggatttgt tagaagaaga aaacaataaa tctctataaa cgatgtctaa gcatctattc
     6901 aacgaatttg aacctgtttc atccaaacaa tggaaacagc aaattcagta cgaactcaaa
     6961 ggggctgatt ataacgaaac tttagtttgg gaaagtccgg aaggtattaa agtaaaacct
     7021 ttttaccatg ccgatgaaaa tcagttaaaa ttcgaatcac caatttcaaa cggatttaaa
     7081 attattcaaa acatttatgt ttatgatatt tcaaagtcta atgaaagagc tgcagaatca
     7141 attaacagag gagcggaatc aattcgtttt acgcttgaat ctgatgctgt ttctttagaa
     7201 aaactaatgg ataatcttcc gaaggaaaat gtcatctatt attttaatgt tttatttcat
     7261 tcgtccgatt ttatcaagaa agccgcctct tttgcctctc aaaacaacta caaaatcttt
     7321 gttttacaag atgctatagg gcaattagct aaggacggaa attggtttga atcattagaa
     7381 aaagattgta accaactgca atctgtagca ttgttaaatt ttccattcct gagttgtaaa
     7441 attgatttat atcaaaatgc aggagcaaat atcattcaac aattggctta tgccatggct
     7501 catgtgaatg aatatttcaa tcgaattgaa ggtatttcaa cgccaataac tttcgaagtt
     7561 tctgttggaa ccaattattt tttcgaaata gcaaaattac gtgccttgcg attacttttt
     7621 gattcgttag caaaagaata tgactataca tttgattgtc atatcattgt cactcctacc
     7681 aaacgcaaca aaactttatt cgattataat gtgaatatgc ttcgtactac tacagaatgt
     7741 atgagtgcta ttttgggagg tgccgatgga atttctaatt tggcttatga tgctctttat
     7801 cacaaagaca acgaattcgg tgatagaatt tccagaaacc aattattggt tctaaagcat
     7861 gaaagttatt tcaatcaagt gcacaacccc gcagacggag cttattatat tgaatcttta
     7921 accgagcaat tagctgaaaa agctttagaa ttatttaaag atattgaagc aaacggcggt
     7981 ttaattacac aattaataga aggaacaatt caacggaaaa tcaacgaaag tgctcaaaaa
     8041 gaacaagact tatttgattc cggaaaagaa gttttgttgg gaaccaataa atatccaaac
     8101 aaaaatgata aaatggcaaa agaattagaa ctttttcctt ttgtaaaaac gaatgcccga
     8161 aaaacgttga taacaccgat tattgaaaaa cgtttagccg aaaaaacaga acaagaacga
     8221 ttatccttag aagcataatt agataaatta acataacggt ttcaatagaa tttataaaat
     8281 gaaacgaaaa aatatacaac agttaacctt gaaaagtgca gtaaaaaaag ctgacactag
     8341 ttctgttgtt cctttcagca cagctgaaca cattgaatta aaacaatctt atcataaatc
     8401 tgacgttgaa aaattagaac atttaggttt tgctgccgga tttgcaccaa atcttagagg
     8461 tccttatgcc acgatgtatg tccgtcgtcc ttggacagtt cgtcaatatg ccggattttc
     8521 tacagctgaa gaaagtaatg ctttttaccg tagaaatcta gcnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnatgccgga ttttctacag ctgaagaaag taatgctttt
     8701 taccgtagaa atctagccgc cggtcaaaaa ggtctttcgg ttgcatttga tttggcaacg
     8761 caccgcggtt atgattccga tcacgaacgt gtggtgggcg atgtgggaaa agcgggtgtg
     8821 gccattgatt cggttgaaga tatgaaagta ttatttgacc aaattcctct tggagaaatg
     8881 tcggtttcca tgaccatgaa cggagcagtt ttaccaatca tggcttttta tattgtggca
     8941 gccgaagaac aaggtgttgc tccaaattta ctttcaggaa ctattcaaaa tgatattttg
     9001 aaagagttta tggttagaaa tacatatatc tatccaccta ctccttccat gaaaattatt
     9061 gcggatattt ttgaatatac cagcaaaaat atgcctaaat tcaattcgat ttcgatttcc
     9121 ggttatcaca tgcaggaagc cggagccacc gcagatattg aattagctta taccctagcc
     9181 gatggtttag aatacattcg aacaggttta gctgcaggaa tggatattga cactttcgct
     9241 cctcgcctat catttttctg ggcaattggc atgaatcatt ttatggaaat cgccaaaatg
     9301 cgagcaggtc gaatgttgtg ggccaaactt ttaaaacagt tcaaccctaa agatgaaaaa
     9361 tcattagcat tgcgaacgca ttgtcaaaca tcaggttgga gcttaacgga gcaagatcct
     9421 tttaataatg tagccagaac gtgtatcgaa gcagccgcag ctgcttttgg aggaacacaa
     9481 tccttacaca ccaatgcatt ggatgaagcc attgccttac caacagattt ttcagctcga
     9541 attgctcgaa atacacaaat atttttgcaa gaagaaacta aaatctgtaa aacggttgat
     9601 ccttgggcag gaagttatta tgtagaaagt ttaactaacg aaattgctga aaaagcgtgg
     9661 gctttgattg aagaagtaga agaattaggc ggtatgacaa aagccattga agccggaatt
     9721 ccgaaattgc gaattgaaga agcctctgcc agaaagcaag ccagaattga cagtggtcag
     9781 gatatcattg tgggtgtcaa caaataccgt ttggagaaag aagacccatt gatgatttta
     9841 gatgttgata atcaaatggt tcgaaaacaa caaattgaaa gtttaaacca aataaaagcg
     9901 agtcgaaata atgaacaagt aaaaatttgt ttagcaaaat tgacagaagc ggcaaaaaca
     9961 ggtactgaaa atttattaac tttagccgtt gaagcagcta gaaacagagc tactttgggt
    10021 gaaatcagtg atgccttaga agaagttttc agcagatata aagcacaaat cagaacattt
    10081 agtggcgtgt atagtaaaga aattaaaaat gatgtaagtt tcgaaaaagc gaaacaatta
    10141 gccgatgcct ttgcaaaaaa agaaggtcgt cgtccccgca ttatgattgc gaaaatggga
    10201 caagacggtc acgatcgggg agcaaaagta gtggccaccg gttatgctga tgttggtttt
    10261 gatgtggaca ttggtccgtt atttcaaaca ccagctgaag cggccaaaca agccgttgaa
    10321 aacgacgtgc acattttagg tgtttcctct ctggctgccg gacacaaaac cttggttcca
    10381 caagtaattg aagaacttaa aaaatatggt cgtgaagaca ttatggtcat agtaggtggt
    10441 gtgattccac atcaagatta ccaattttta ttcgatgcag gagcagttgc tgtttttggc
    10501 cccggaacga aaattagtga agcagcaatc agtattttgg aagttttatt ggaagattaa
    10561 taattatatt attggtttga aaaacactga ctgagaggtt ggtgtttttt ttatctttac
    10621 aaaaaaaata aaatgctcac cctctccttt actccattcc ccattctaga atccaatcgt
    10681 ttgttactgc gagcggtttc aactgatgat gttaatgaaa tattggaact acgtggtaat
    10741 ccggaaacga tgaaattcat tccgagaccc ttagttacct ctacagaaat ggcattggaa
    10801 catattggaa tgattaccga aaaaattgaa aacaatatcg gtatcaattg ggcaattact
    10861 gtaaaaggaa atccaaaatt actcggtgtt attggtcact atcgcattca aaccgaaaat
    10921 taccgttcgg aaattgggta tatgttgttg cccgaatacc aaggtcaagg tattgtaagt
    10981 gaagccatta atatggtttt agagtatggt ttcaatgtgc taaattttca ttcagtagaa
    11041 gcggtagtgg acccggaaaa tcatgtttct gcaaaagtct tagagaaaaa tggatttgta
    11101 aaagaagccc actttttaga aaacgaatat tgggacggca agttttggga ttcagcggtc
    11161 tattcgcttt taaaaagaaa ttttgttaag aaataaactt tttaactcaa ttagttttaa
    11221 aacatttcta tttttacaat atgtctaaac ttccgcactt gcaaaccagc attttcactg
    11281 tcatgtctaa aatggcaata gatttcaatg caataaattt atctcaagga tttccaaatt
    11341 ttccgattga tgaaaaaatg gctgagatta taagacgatt agccggttca gatgtgcatc
    11401 aatacacgcc aatggcaggt tatccgtctt tgatggaaaa aattgccaaa ctaaccgctc
    11461 aatcctacca aagagccatc aatcctttga cggaactgtt aattactgcg ggagctacag
    11521 aggctatttt tgcaaccatt caagctttag ttcattctgg tgatgaagta ttgatttnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct atttttgcaa ccattcaagc
    11701 tttagttcat tctggtgatg aagtattgat tttggatcca agttatgatt gttatgaaac
    11761 ccccatttta ttgagcaatg ccaaaccggt tcgcgtttct ttaaatgagg attattcccc
    11821 aaattttgag aaaattgctc atcatttatc gtctaaaact aaacttctaa ttttaaataa
    11881 tccgcacaat ccctccggaa aaatgtggtg tgaaaaagag tttgttgaat tagaaaattt
    11941 gttagaaaaa tttcctgatg ttttactttt atccgatgaa gtttacgaat acatagcttt
    12001 agaaaacaac cacatttctg tcaatacaag agaaaaactg cgtcatcggg caattattac
    12061 ttcttctttt ggaaaaacat tacacctaac tggatggaaa attggttatg ccgttgctcc
    12121 tgaacatttg atgaaagaag taaaaaaagt gcatcaattt ttagttttta gtgtcaacag
    12181 tctatctcaa gctgccataa gtgattattt agatattttc gacatcagtt cagtcaaaac
    12241 agtataccaa caaaaaagta atttttttca aaacttaatg aaagaaacat cttttgaatt
    12301 attaccctgt gaaggaactt attttcaagt tgcttcttac aaaggcattt caactgaatc
    12361 tgatttggat ttcactaaac gattagtaac tgaatttggt gtttcaacca ttccgctatc
    12421 tattttttat ggtgataata ccgatttaaa acgcattcgc ttttgctttg ccaaagatga
    12481 tgaaacactc acaaaagcag cagaacgatt acttaaatta aaataataac gttacataaa
    12541 ctataaatta ctttcagaat tatagctatt ttatatcttt acaaaaaacc aactatttat
    12601 gaatttttca gcaaaaatgc ttcgtgataa tgccctagaa ggcaaagtaa ttgtcgtaac
    12661 cggtggcggt agcggactcg gaaaagcgat gacaaaatat ttcttagaat taggagcaaa
    12721 agtagccata acttccagag atttagacaa gttaacaaca accgctgctg aattagaatc
    12781 acaaaccgga ggaatttgtt tgccattaca atgtgatgtt cgccattatg atcaagtgga
    12841 agcaatgtta caagaagtat taaaaacatt tggaaaagcg gatgtattaa tgaataatgc
    12901 tgccggaaat ttcatctcac caacagaaag attatcggcc aatgcttttg atacgattat
    12961 tgacatcgtt ttaaaaggtt caaaaaactg caccttagcc tttggaaaac attggattga
    13021 taccaaacag gaaagttctg taattttaaa tattgtcact acttatgcat ggacaggttc
    13081 tgcttatgtt gtacctagtg caacggcaaa agcaggtgtt ttggctatga ccagaagttt
    13141 ggctgtagaa tgggcaaaat atggtattcg tagcaatgca attgcacccg gaccttttcc
    13201 aaccaaaggg gcttgggacc gattattacc gggcgattta gccgaaaaat ttgatatggc
    13261 caaaaaagta cctttgaaaa gagtgggcga tcaccaggaa ttagccaatt tagcggctta
    13321 tttagtatct gatttttcgg cttatgtaaa tggtgaagtt attaccattg acggtggcga
    13381 atggttaaaa ggtgccggtc aatttaatct tttagaagat attcccgaag aaatgtggga
    13441 tatgttagaa gctatgatta aagcgaagaa aaacggttaa ttcaataaga atttgtatcc
    13501 cttttttcga tttaaaagct aatcatttga aggacaaaac aattaacatt tgttaacaac
    13561 ttgaagttga aaaacccata aataatcgta attttacggc tcaaaatttt ataaaatcaa
    13621 tgggtaaaat aattgcaatt gctaaccaaa aaggaggtgt tggaaaaact acaactaccg
    13681 taaatttagc ggcttcttta ggtgttttag aaaaaaaagt attactgatt gatgctgacc
    13741 ctcaagcaaa cgcaagttca ggattgggga ttgacgttga ttctgtcgcc attggttcct
    13801 atcaagtttt agaacacagt aatactgctg ctgaagcaat aatgaaatgt tcagcaccca
    13861 atgtagatat tattccggca cacattgatt tagttgccat agagattgaa ttggttgaca
    13921 aagaaaatcg agaatatatg ctgaaagaag cattagcatc gataaaagac caatatgatt
    13981 acattttaat tgactgtgct ccttccttag gattattgac attgaacgca ttaactgctg
    14041 cagacagtgt aataattcca attcaatgtg aatattttgc attagaaggt ttaggtaaat
    14101 tattaaacac aatcaaaagc attcaacgat tacacaatcc cgatttagat attgaaggat
    14161 tattattaac catgtttgat tcccgcttgc gtttatcaaa tcaagtggta gaagaagtac
    14221 aaaaacattt caatgacatg gtatttaaaa ccattattca acgaaatgta aaactaagtg
    14281 aagctccaag ttttggggaa agcatcatta attttgatgc aaccagtaaa ggagcaaaca
    14341 attatttaag tttagcagaa gagattatca aaaaaaatag ctagtatttt atgacacaag
    14401 ccatcaaaaa gcaagcatta ggaagaggat tatccgcttt attaaaagat cctgaaaacg
    14461 atattaaatc agttaatgat aaaaatgccg ataaagttgt tggtaatatc atcgaacttg
    14521 aattagaagc aattgaaatt aatccgtttc aaccacgaag taattttaat gaagaaacac
    14581 ttcaggagct ggcttcatcc attaaagaat taggcgtaat tcaacctatt actgtaagaa
    14641 aactagaatt taataagtac caattaattt caggagaacg aagacttcgg gctgcaaaaa
    14701 tagccggttt aaccgtcatc ccctcctata ttcgaattgc cgatgataat gaatcattgg
    14761 tgatggcttt ggttgaaaat attcaacgtc atgatttaga ccctattgaa attgctttat
    14821 cttaccaacg gttgattgat gaaattcaat taacacaaga gcaaatgagc gataaagttg
    14881 gaaaaaaacg ttcgacaatt gcaaattact tgcggttatt gaaattagac ccaattatac
    14941 aaaccggtat tcgggatggt tttatttcca tgggacatgg tcgagctttg attacaattg
    15001 aaaatgagga tatacaatca gatatctatc ataaaattat cagtcaaaat ctttctgtgc
    15061 gggaaactga aaatgtcgta aaaaattatc aggaaagttt aaaaccaaaa tcaggaaaga
    15121 caataaaagc taatccttct ttttccgttt cagaaatgga taaaaaggaa attggcacca
    15181 cgttaggcac aaaagttgac ataaaaattg atgcgaaagg aaaaggaaaa atcaccattc
    15241 ctttcaaatc tgaatctgat tttcaacgca ttatagcttt aataaaagga tagtgcatag
    15301 cttaagaatc atattttttg ctttttccct tttggggaca acttttgcta atgctcaagt
    15361 tcaaattgaa gaatttggcc aaacccaaga cacgttaaaa tacaaaccaa ttgacccttt
    15421 ggcaccttca aaagctgcct tttattctgc catactgccg ggtttaggac aagcatataa
    15481 taaaaaatac tggaaaatcc ccattgttta tggtggatta gctattggtt attattttta
    15541 taatgataat aataaatatt ataatgaatt cagagatatt tataaaagac gtttagaagg
    15601 ctacacagac gataaatatt atggtattta ctctgataat gtattgatta atgctcagcg
    15661 tacttatcaa agaaaccgtg atatttcact aatggttatg attggtattt atgtattaaa
    15721 cattatagat gcaaatgttg atgctcattt aatacaattt aatgtcaacg agaatctatc
    15781 cttaagacct gacattaata taaatgacat cactagaaaa caaaatttag ggttagtatt
    15841 aaactataaa ttctaatgaa aatagcactg ttaggttatg gaaaaatggg aaaagttatc
    15901 gaacggatag ctttagaacg cggacatgaa attgtattgc gaaaatcgag ttccgattcg
    15961 tttgaaggat tagaattagc agatgtcgcg attgatttca gtattccaaa tgcagccgtt
    16021 tcaaatattt cctattgcat cgaacaagga attcctgtag tttcaggcac aacaggctgg
    16081 ttagaaaatt accatacaat ggttcaactt tgtcaatcga aaaaagcagc atttatttat
    16141 ggttctaatt ttagtttagg agttaacttg tttttcgaat taaatgatta tttagctaaa
    16201 atgatggcta aattcaatca atataatgtt tcaatggaag aaattcatca cactcaaaaa
    16261 cttgacaaac caagtggaac tgccatttcg ttagctaatt caattataaa tcataccgat
    16321 aaaaatagtt ggagtattga aaacccacta aaagatgatt tatttattga tgtcaaaaga
    16381 gtggataatg taccgggaac tcacactatt ttttatacct cagcagtaga tactatcgag
    16441 attaaacata ctgctcacaa tcgggatggt tttgcctttg gtgcaattat tgccgcagaa
    16501 tggattgttg gtaaaaccgg tgtttttaca atgaaagatg ttttaggatt aaaataataa
    16561 ttaaaagtta aattggtaac aatttttctt gagatacaac taatacaatt tagccagttt
    16621 attaaaaaca aacttaaatt tttataaaat gacattatat cagtggttta tatttttctt
    16681 agtgattcaa ctcgtgcatt atatctgcac ttggaaatta tatcaaaaag caggtcgaca
    16741 atcttgggag gctgccatac cggtttataa tgccgttata ttgatgaaaa taattaatcg
    16801 cccatggtgg tggacttttt tattatttat ccctattgtc aatttgatca tgtttccggt
    16861 gatttgggta gaaaccttaa gaagttttgg taaacattct tctttagata ctcttttggc
    16921 ggtgataaca tttggcttgt atattggtta tgtgaattat acccaagaag ttacccacat
    16981 aaaagaacgt tcattacacc ctaagaatcc aacagcagat tttgtaagct ctattttatt
    17041 tgccatagtt gtagcaacca cagtgcatac gtatgtcatg caaccctata ctatccctac
    17101 ttcatcttta gaaaaatcgt tattgattgg agactttctc tttgtaagta aatttcatta
    17161 tggggcacgt actccaacaa cggcaattgc tcttccaatg gttcacgata gcataccatt
    17221 tgtaaaaaca aaatcctatt ttagttggcc acaactaccc tattttagat ttccgggtat
    17281 acaaagtgtt gaacgcaatg acatcgtggt ttttaattgg ccaaaagata ctgtttataa
    17341 atttttcgac agatccggaa aaagagcaga caaaccactt gataaaaaat ctaattacgt
    17401 taaacgctgt actgccattg caggagatag tcttcaaatt ataaaaggag atgtttttgt
    17461 taatggaaaa ttactcccgc ttcacgaccg tcaaagatta caattttctt ataatgttga
    17521 aacggacggc tcacaattta atgaagaata tatatttaaa gaattacaaa tcaccgatgg
    17581 tatttatgtt aattcacaaa attcatatac tttttcagca ttaacannnn nnnnnnnnnn
    17641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17701 nnnnnnnnnn nnnnnnnnnn nnnnnngatc atattgttgg aaaaccggta tttatttgga
    17761 tgagtttaga ccaaaatgaa ccttggggaa aattatttga caaagttcgt tgggaaagaa
    17821 tgtttactac cgttggaggt agcggaaaac ccgtatccta ttttatctat tttgtaattg
    17881 ccttggtagc ttggtttggt tttgattatt tcagaaaaaa aaggaaaaaa tcagctgaat
    17941 aaatttcatt atgaaggctt tattatatcc aacctacttt ccttccatta gccattatgc
    18001 tgcgatggtt caagctgatt cgattacctt tgaaacggaa gataatttcc agaaacaaac
    18061 taatcggaac cgaatgtata tttatagtcc gaatggaata caattgctca atatacctat
    18121 aaaacattct aaggaagctc accaaaaaac gaaagaagta aaaatagagc aagatttcaa
    18181 ttggcaaaaa caacatttca aatctcttga agcagcctat cgaacatctc ctttttttga
    18241 atattttgaa gatgatattc ttccggtgtt taaaaaaaaa caaacttttc tattagattt
    18301 aaacctagaa gtttttgaga tagttaacca atgtttaggc ataactttac cattcgaaaa
    18361 aacagcggag tactttcaaa acagttcgga ttatattgat tttcgttctt tggctaacgg
    18421 taaaaaagac caaaccagct gtgaacgtta cacacaggtt tttgaagaaa aacatggttt
    18481 tttgaacaac ctaagtattt tagatttact ttttaacgaa ggtcgatatg ccttagatta
    18541 tttaaaaaat cagaagttat aattattcaa tttttttaaa tgcgagacgc gttacgattg
    18601 gaaaatgatc tgaattaaca aattcagaat gagtagtaaa ttgtttaacc aaaaacagtt
    18661 tgtcagtaaa aacatagtct attctacccg gataatattt aaaattatac gattttccaa
    18721 agcctgaacc tgcttcttca aaagtatcat ttaagtcacc tttaatactt ctatatacaa
    18781 aagaataagc actattattt aaatccccac agataataac aggatgttta cagtctaatt
    18841 tatgcttctt aattatctca gcttgttgct gttgcgtttt aaaagaggca ctcattcgtc
    18901 tgaaaatacg tttggattga tcttgagaaa caccttgtat attttcatca atttcatgca
    18961 catcaggtgt tattttaatt gattgtaaat gtatgctgta aacccttatc gtatctttcc
    19021 ctcttttaat atcagcataa acagcattat ttgttgaatt tggaaactca ataattccct
    19081 cttcaatgat ggggtatttt gaaaaaattg cctgacctaa tttggtattt tttccttcaa
    19141 caaaaatata tttatgttta taaattctaa aatcaacatc attatttctg gaatattcct
    19201 gcagacataa aatatcagga ttttgttctt taataaagga agaaacttga ctactcacat
    19261 cgtcacgttc aatccattta tacaaattga ataaacgaac gttataactc attaaaacaa
    19321 aatcttcatc ttcgattggt aaattagttt ccgaaaattt ataaaattta gttacgaatg
    19381 taattcctaa taacaaaaca atcccggaaa gcaacatttg ttttttgaat tgcagcaacc
    19441 aatacacaaa aaataataaa ttaaaaatca acatcaatgg taaaactaaa gtcagcacag
    19501 aaagtatcgg aaacaattta ggagccaaaa aaggcaaaac ataagcaatg aaagtcgtta
    19561 tagttaatac tatattgaaa aaaaacatca ctttattaaa ccaatttagg tttttcatat
    19621 tttatttatt caattactaa aaccaaatta attgcactta ttaacttttt ccggctttaa
    19681 acaaaaattc tttttcgtct tttgttaaac tatcataccc tgatttactg attttatcta
    19741 aaatttcatc aatctgctgt tgtgatttat cttttggact aggtttaggt tttgtatata
    19801 ttgtgttttt atgtactttt ttaaacggtg tattcttttt agctttaaac aaatttacaa
    19861 tactattcat taaggaagta aatccttttg taatatcagt tcctttttta agttgactag
    19921 cgtataaata gccaaataaa gcacctccta aatgagcaat gtgaccgcct acatttgtat
    19981 aggataaact tattaagtca atagccagat aaaaaacagc catgtgccag agtttaatat
    20041 tacctattaa aagcaagcga atttgcatgt ggggagcata agacgaaaca gcaaataaaa
    20101 cagccataat agcacctgaa gctccaacta atgttactaa ttgattcgac aatgcaggaa
    20161 aaaacaaata acttaaaata taaattaatc cagcaaaaat ggcacccatc aaatataagc
    20221 tcaataattg tttttgggta aaaaaggtca aaaacaatct cccaacaaag tttagaataa
    20281 tcaaattaaa aagaatatgg aaaatatctg catgaaaaaa agcataagaa ataattgacc
    20341 aaggtttcca tgccaaatca cttgggttag aagataaact gatataatct gtaaaattaa
    20401 aaccaattcc aaacaaagac aacaaacctt ttattatcca tggaatagca aacaaagcca
    20461 cattccagta gattaacttt tcagttacac cacctaaaat atagtgttgt tttaattcct
    20521 ttaaaatact catttcaatt accttttata aattaccatc cacctttatt aattaccttt
    20581 ctcaatccca ccgcttttca ttaaactgat ttttcttcca aaaccacatc attaaaaatc
    20641 cggttaaagc tccacctacg tgagcaaaat gggcaattcc accaccacca aaaatagatt
    20701 ctccttttaa tcccatgaat aaatcaccta aaactaatac cggaacaaaa tatttcgctt
    20761 tgatgggtat tggtaaaaac aacaacatca attctgcatt tggaaacata aaagcaaaag
    20821 caacaattac accatacaat gctcctgatg ctccaacagc tgctgtttga tatttgatag
    20881 ctgcacgaac aataatatta tattgctctt gattagcatt tactttttca agaatattat
    20941 ttaaacttcc gtcaataatt ttattattaa catcaaattg attagtatga tcaatattca
    21001 ttaatatgtg catatcagaa gctgaaatat ttaattcaga aacttgttgt aacaatgaat
    21061 taatctcata atagttcatt ccggtgtgta gtaaagctgc acccaatcca caagaaatat
    21121 aaaaaaacaa aaactttttc catccccaaa aatgttccaa agccgagcca aacatgatta
    21181 atccgaacat attaaagaaa atatgcatca aattaggcan nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnnnnnn nnnnnnnnng aggcttgttg aactgtcatg ttcttcaaat actgctcata
    21361 caaaattcgt tgatgagctc ttttttggtc aatcaccatc atgccggatt taatggaact
    21421 caaaatgtat tttttctgaa tttgataagt tctgttttta gattcctcta tttctgtatc
    21481 gttaaaaagg gagcttgata ctgcttcctg ttcaaatgaa aatgattcta tttccttggt
    21541 gtcatttttt aaaccaacat acaaactttc ccaatgagca ttttccgttt ttctttctct
    21601 gtaagttgaa cctccaaaat tcattgttgc tttttcttcc gcaaacggat taaaattaga
    21661 attaacctga attaatggcg tcgaagcatc tttattttgg taatgataag gtgtgtctaa
    21721 attggggtca cgttcaaaat ccaatacagg agccacatta aattgaccta aactatgttt
    21781 tacagtagaa cgtaaaattg catataatgc atgttcatca tcaaacttaa tttcggtttt
    21841 agtaggatga atattgatat caatcgtatt tggtggcact tctaaataaa taaaatagcc
    21901 cggttgcata ccatctttga gtaaaccttc ataagcagcc atcactgcat gatgcaaata
    21961 accgctttta atatatctat tgttaacaaa aaagaattgc tcacttctgt tcttttttga
    22021 aaattcaggt tttgaaataa aaccattcac ttgaacaatt tcagtttctt cagaaatagg
    22081 gactaatttt tcgttggttt tccctgaaaa aatagcgact atccgttgtc ttaaatttga
    22141 agagtgcaaa ttaaacaact cacttccatt atgaataaac gtaaaatgaa tggaaggatg
    22201 agctaaagca actctctgaa attcatcaac aatatggcga tgttctactg tttcagactt
    22261 aagaaaatta cgccgtgccg gaatattaaa aaacaaattc tttacggcaa aggatgttcc
    22321 ttttggcaaa acagaaggat cttgcgttat aaatttactt ccttcaataa taatatgcgt
    22381 tcctaattca tcaaactctt gttttgtttt taattccaca tgagcaattg cggcaattga
    22441 agctaaagct tcacctctaa atcctttggt atgaattgta aacaaatctt cagcatgacg
    22501 aattttagaa gttgcatggc gttcaaaaca cattctggca tcggtaacac tcattccatt
    22561 accgttatca attacttgaa ctaatgattt tcctgcgtct tttactatca gtttaatatc
    22621 tgtagctttt gcatcaactg cattttctaa caattctttt accacagatg ccggtcgttg
    22681 aacgacctca ccggcagcaa tttgattagc cacatgatcg ggaagtaatt gtataatact
    22741 cgacattatc ttttgataaa tatggaaaga tcgaaatcga taatgaataa aaaaatcaat
    22801 agtaatacta agattatggc aattaatctg aaatttattt ctctgttacc acggtgtcta
    22861 ctgtcatttc tagcatcact ccaatgtgct ctgaagtcat tatagtttac cgtttctcgg
    22921 tttttattaa acttagaatc aaaatcatac aagttgccca gctccttccc ttcataatgt
    22981 ctaggagtat aattgaaact tctgtttttg ggcaatttga ataacgattt tttaatcatt
    23041 gtgttttgta ttaaccttca aagttaagta attctcaatt gacttgaaaa tttgaatatg
    23101 tgaaagtttt gttacttttc aacaaaaaaa gtaaaaatta aaaagtagaa attgtaaact
    23161 aaattatctt tttaatgaaa aatgcccctt aattgttcga ttgtcatcta aactgattac
    23221 ataccaataa tcatctgccg gtaaaggatt accattaatt gttccgttcc atccttgatt
    23281 gatgggtgta atttgaaata acaatttgcc gtatcgatca aaaatgtcta tttttacttt
    23341 tatattagaa tcttgggtaa ttcctctcaa gttccataaa tcattgtatc catcagaatt
    23401 tggagtgaaa aatttaggaa tgcttaatac gttgatattt tcagtttcaa ttccgcaatt
    23461 atataaatct tttacgtaaa tcgtaattaa cccgggctca atatttgtaa aaatagttga
    23521 ctcttggaac acgacaccat caatactata aacataattt ccaattcccg aaacaactat
    23581 aacaattgaa ttttcatcaa ttaaatcatt tacaataata tcttcaatta ctgcaatttc
    23641 ggaatccatc acttgaatgg ttctcgtttt a
//
LOCUS       DAOTCW010000020        23472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1129_length_23472_cov_42.556177, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000020 DAOTCW010000000
VERSION     DAOTCW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 23472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23472
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1129_length_23472_cov_42.556177"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1091
                     /locus_tag="P5335_02910"
     CDS             105..1091
                     /locus_tag="P5335_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ73864.1"
                     /translation="MKTTLKTVLLLFFTTSCFSQIYYSNYLDETSEWREISKMVNQAG
                     AYYFTFSTRFFEGFENINGFTYYKMYKTKYTKGYYIGDYSPFFISDEETSFLGFVRED
                     NSGKFYTGTSNGNENLFFENQLVIDAQIGDSFSTLNVYSAFGQESSCTIENISFISLS
                     GMNLKVVSDENENSLIKGATVIEGIGRVYPYCESPQILDNVISNDQERIFCYTKQGQI
                     YQYENDYNLFDMVLNCDSFPNPNRQGLSVSNFNTNAIQIFPNPTTSTINIKINKENII
                     SIQLFDVEGRVLEDINGNESSFQFDLSNYRNGTYLVKVITERGSKVVKILKI"
     gene            1339..1746
                     /gene="rpsL"
                     /locus_tag="P5335_02915"
     CDS             1339..1746
                     /gene="rpsL"
                     /locus_tag="P5335_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRZ73865.1"
                     /translation="MPTIQQLVRTGRAQITKKSKSAALDSCPQRRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNGNEVNAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DTAGVAGRTQRRSKYGAKRPKPGQVAAPVKKKK"
     gene            1771..2247
                     /gene="rpsG"
                     /locus_tag="P5335_02920"
     CDS             1771..2247
                     /gene="rpsG"
                     /locus_tag="P5335_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRZ73866.1"
                     /translation="MRKRQAKKRPLLPDPKFNDQLVTRFVNNLMWDGKKSTAFKVFYD
                     ALEIVDAKKQDAEKSSLETWKDALTNVMPHVEVRSRRVGGATFQIPMQIRPDRKISYA
                     IKWMILYARRRNEKSMAAKLASEILAAAKEEGAAVKKRMDTHKMAEANKAFSHFRF"
     gene            2258..4414
                     /gene="fusA"
                     /locus_tag="P5335_02925"
     CDS             2258..4414
                     /gene="fusA"
                     /locus_tag="P5335_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRZ73867.1"
                     /translation="MARDLKYTRNIGIAAHIDAGKTTTTERILFYTGKSHKIGEVHDG
                     AATMDWMAQEQERGITITSAATTCEWNFPTEQGKVLPETLPYHFNIIDTPGHVDFTVE
                     VNRSLRVLDGLVFLFSAVDGVEPQSETNWRLADQYRVPRMGFVNKMDRQGSNFLGVCQ
                     QVRDMLKSNAVAITLPIGEEIDFKGVVDLVKNQAIVWHDETLGATFDVVDIPADMVDE
                     VKQYRSILIEAVADYDENLLDKYMEDENSITEEEINNALRAATMDMAIIPMIAGSSFK
                     NKGVQFMLDAVCKYLPSPMDKEGIVGIHPDDQDLLEEDQRKILRKPDVKEPFAALAFK
                     IATDPYVGRLAFFRAYSGRLDAGSYVLNTRSDNKERISRIYQMHANKQNPIDYIEAGD
                     IGAAVGFKDIKTGDTLCDEKHPIILESMKFPDPVIGIAIEPKTKADVDKMGMALAKLA
                     EEDPTFTVRTDEASGQTIISGMGELHLDILVDRMKREFKVEVNQGEPQVEYKEAFTKA
                     AQHRETYKKQSGGRGKFGDIVFKLGPADDVDGKPYVGLQFVNEVKGGNVPKEYIPSVE
                     KGFKEAMKTGPLAGYAVDSLKVTLLDGSFHPVDSDALSFELAAKMGYKEVAKAAGAVI
                     LEPIMKIEVITPEENMGDIVGDLNRRRGQVNDMGDRAGAKTIKAFVPLSEMFGYVTTL
                     RTLSSGRATSTMEFSHYEQTPSNISEEVIKKAKGNA"
     gene            4426..4731
                     /gene="rpsJ"
                     /locus_tag="P5335_02930"
     CDS             4426..4731
                     /gene="rpsJ"
                     /locus_tag="P5335_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRZ73868.1"
                     /translation="MSQKIRIKLKSYDHMLVDKSAEKIVKTVKSTGAVVTGPIPLPTH
                     KKIFTVLRSPHVNKKSREQFEVSSYKRLLDIYSSSSKTIDALMKLELPSGVEVEIKV"
     gene            4845..5462
                     /gene="rplC"
                     /locus_tag="P5335_02935"
     CDS             4845..5462
                     /gene="rplC"
                     /locus_tag="P5335_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRZ73869.1"
                     /translation="MSGLIGKKIGMTSIFDENGKNIPCTVIEAGPCVVTQVRTKAVDG
                     YEAFQLGFDDKTEKHATKADLGHFKKAGTSAKKKVVEFQEFETEYKLGDLITVDLFSE
                     GEFVDVQGVSKGKGFQGVVKRHGFGGVGQATHGQHNRLRAPGSVGASSYPSRVFKGMR
                     MAGRMGGDNVTIQNLRVLKVVADKNLLLVKGCVPGHKNSYVIIQK"
     gene            5462..6091
                     /gene="rplD"
                     /locus_tag="P5335_02940"
     CDS             5462..6091
                     /gene="rplD"
                     /locus_tag="P5335_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRZ73870.1"
                     /translation="MEVKVLDINGKETGRKVQLSDSVFAIEPNNHAIYLDVKQYLANQ
                     RQGTHKAKERAEVTGSTRKIKKQKGTGTARAGSIKNPLFKGGGTVFGPRPRSYSFKLN
                     KTLKRLARKSAFSQKVKDSELVVVEDFNFDTPSTKNFITVLKALGLENKKSLFVLGDS
                     NKNVYLSSRNLKGSRVVTNSELSTYAILNTNKLVLMESSLEGIEENLSK"
     gene            6098..6388
                     /gene="rplW"
                     /locus_tag="P5335_02945"
     CDS             6098..6388
                     /gene="rplW"
                     /locus_tag="P5335_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRZ73871.1"
                     /translation="MSIIIKPIITEKITKQSELNNRYGFVVDRKANKIQIRNAVEAAY
                     GVKIVAINTMNVRPDRSVKYTKSGMISAKSNAYKKAIVEVQEGETIDFYNNI"
     gene            6396..7220
                     /gene="rplB"
                     /locus_tag="P5335_02950"
     CDS             6396..7220
                     /gene="rplB"
                     /locus_tag="P5335_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRZ73872.1"
                     /translation="MSVRKLKPITPGQRFRVVNGFDAITTDKPEKSLLAPKNNSGGRN
                     SQGKMTMRYTGGGHKQKYRVIDFKRTKAGIPATVKSIEYDPNRTAFIALLSYVDGAKT
                     YIIAQNGLNVGQTVTSGEGAAPEIGNALPLSKIPLGTVISCIELRPGQGAIIARSAGT
                     FAQLMARDGKYATIKMPSGETRLILLTCMATIGAVSNSDHQLIVSGKAGRSRWLGRRP
                     RTRPVAMNPVDHPMGGGEGRSSGGHPRSRNGIPAKGYRTRSKVSPSNKYIVERRKK"
     gene            7231..7509
                     /gene="rpsS"
                     /locus_tag="P5335_02955"
     CDS             7231..7509
                     /gene="rpsS"
                     /locus_tag="P5335_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRZ73873.1"
                     /translation="MARSLKKGPFVHYKLDKKVLENIEKSSKAVIKTWSRASMITPDF
                     VGQTIAVHNGRQFVPVYVTENMVGHKLGEFSPTRSFRGHAGAKNKGKK"
     gene            7516..7926
                     /gene="rplV"
                     /locus_tag="P5335_02960"
     CDS             7516..7926
                     /gene="rplV"
                     /locus_tag="P5335_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRZ73874.1"
                     /translation="MGVRKRETADARKEANKQIAFAKLNNCPTSPRKMRLVADLVRGQ
                     KVERALNILRFSQKEASRKLEKLLLSAIANWQAKNAEANIEEAALFVKEIRVDGGMML
                     KRLRPAPQGRAHRIRKRSNHVTIVLGASNNTQSN"
     gene            7932..8678
                     /gene="rpsC"
                     /locus_tag="P5335_02965"
     CDS             7932..8678
                     /gene="rpsC"
                     /locus_tag="P5335_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRZ73875.1"
                     /translation="MGQKTNPIGNRLGIIRGWDSNWYGGNDYGDKIAEDYKIRKYIHA
                     RLSKASVSKVIIERTLKLVTVTITTARPGIIIGKGGQEVDKLKEELKKITDKEVQINI
                     FEIKRPEIDAYLVATSIARQIESRISYRRAIKMAIAAAMRMNAEGVKVLISGRLNGAE
                     MARSEGFKEGRIPLSTFRADIDYALAEAHTTYGRMGIKVWIMKGEVYGKRDLSPLVGM
                     DKKQAGGGSKGGDAPRGGKPNGKSNPRKRK"
     gene            8698..9117
                     /gene="rplP"
                     /locus_tag="P5335_02970"
     CDS             8698..9117
                     /gene="rplP"
                     /locus_tag="P5335_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRZ73876.1"
                     /translation="MLQPKRTKYRKVQKGRMKGDSQRGHELSNGMFGIKSMDSSFLTS
                     RQIEAARIAATRYMKREGQLWIKIFPDKPITKKPLEVRMGKGKGAVEYWAAVVKPGRI
                     MFEVGGVPLSVAKEALRLAAQKLPVKTKFVVARDFEA"
     gene            9130..9321
                     /gene="rpmC"
                     /locus_tag="P5335_02975"
     CDS             9130..9321
                     /gene="rpmC"
                     /locus_tag="P5335_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRZ73877.1"
                     /translation="MKQSEIISLSSAELQEKLSQSRKTYADLKTTHTISPIQNPLQIK
                     AVRRTIARLVTELSKRELQ"
     gene            9339..9596
                     /gene="rpsQ"
                     /locus_tag="P5335_02980"
     CDS             9339..9596
                     /gene="rpsQ"
                     /locus_tag="P5335_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRZ73878.1"
                     /translation="MEKRNLRKERVGVVTSNRMEKSIVVSETRKVKHPLYGKFVLKTK
                     KYVAHDETNDCNIGDTVRISETRPLSKSKCWRLVEIIERAK"
     gene            9599..9967
                     /gene="rplN"
                     /locus_tag="P5335_02985"
     CDS             9599..9967
                     /gene="rplN"
                     /locus_tag="P5335_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRZ73879.1"
                     /translation="MVQQESRLKVADNTGAKEVLTIRVLGGTKRRYASVGDKIVVSIK
                     DATPNGNVKKGAVSTAVVVRTKKEVRRADGSYIRFDDNACVLLNAAGEMRGTRVFGPV
                     ARELREKQFMKIVSLAPEVL"
     gene            9979..10293
                     /gene="rplX"
                     /locus_tag="P5335_02990"
     CDS             9979..10293
                     /gene="rplX"
                     /locus_tag="P5335_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRZ73880.1"
                     /translation="MTKLKIKSGDIVRVIAGDHKGAEGKVLRVFIDANKAIVEGTNMV
                     SVHTKPSAKNPQGGIVKKEAPIQVSNLSLIDPKSKKATKVGFKVEGDKKVRFSKKSNQ
                     VL"
     gene            10296..10847
                     /gene="rplE"
                     /locus_tag="P5335_02995"
     CDS             10296..10847
                     /gene="rplE"
                     /locus_tag="P5335_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRZ73881.1"
                     /translation="MAYIPRLKEEYKSRVIPALKEEFGYKNVMQVPKLEKIVLSRGVG
                     AAVSDKKLVDYAVDELTKITGQKALATISKKDVASFKLRKGMPIGAKVTLRGERMYEF
                     LDRLITSSLPRVRDFGGIKATGFDGRGNYNLGVLEQIIFPEIDIDKVNKISGMDITFV
                     TTAKTDKEAKSLLAELGLPFKKN"
     gene            10851..11120
                     /gene="rpsN"
                     /locus_tag="P5335_03000"
     CDS             10851..11120
                     /gene="rpsN"
                     /locus_tag="P5335_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRZ73882.1"
                     /translation="MAKESMKAREVKRQKTVEKYAEKRKALLEAGDYEGLQKLPKNAS
                     PVRLHNRCKLTGRPRGYMRQFGLSRVTFREMANQGLIPGVKKASW"
     gene            11194..11592
                     /gene="rpsH"
                     /locus_tag="P5335_03005"
     CDS             11194..11592
                     /gene="rpsH"
                     /locus_tag="P5335_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRZ73883.1"
                     /translation="MYTDPIADYLTRVRNAVKANHKVVEIPASNLKKEITKILFDQGY
                     ILSYKFDDTSVQGSIKIALKYDKVTKEPVIRDIQRISKPGLRKYAGSSNLPRILNGLG
                     IAIVSTSKGLMTGKQAKQLNVGGEVICYVY"
     gene            11608..12150
                     /gene="rplF"
                     /locus_tag="P5335_03010"
     CDS             11608..12150
                     /gene="rplF"
                     /locus_tag="P5335_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRZ73884.1"
                     /translation="MSRIGKNPIVIPAGVTLEVNEGVITVKGKLGQLTQEFSDVTVTV
                     EDGQVLVERSSDHKDQRAKHGLYRSLISNMVNGVSTGFTKELELVGVGYRASNQGQKL
                     DLALGFSHNIVLEVAPEVKLETISEKGKNPIVKLTSHDKQLLGQVAAKIRGFRRPEPY
                     KGKGVKFVGEVLRRKAGKSA"
     gene            12162..12515
                     /gene="rplR"
                     /locus_tag="P5335_03015"
     CDS             12162..12515
                     /gene="rplR"
                     /locus_tag="P5335_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRZ73885.1"
                     /translation="MSLTKSDRRQRIKFRIRKIVSGTATKPRLSVFRSNKEIYAQLID
                     DLNGVTLLAASSRDKGVDAKGTNIEVASAVGKLVAEKALKAGIDTVTFDRGGYLYHGR
                     IKSLADGARAAGLKF"
     gene            12522..13046
                     /gene="rpsE"
                     /locus_tag="P5335_03020"
     CDS             12522..13046
                     /gene="rpsE"
                     /locus_tag="P5335_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRZ73886.1"
                     /translation="MYNKYKNVELVKPSGLELKDRLVSVNRVTKVTKGGRAFGFSAIV
                     VVGDENGVVGHGLGKSKDVSEAIAKAVEDAKKNLVKIPLSGQSVPHEQKGKFGGARVF
                     LMPASHGTGVIAGGAVRSVLESVGIHDVLSKSQGSSNPHNVVKATFDALLQMRSAYTV
                     AKQRGISLEKVFKG"
     gene            13061..13243
                     /gene="rpmD"
                     /locus_tag="P5335_03025"
     CDS             13061..13243
                     /gene="rpmD"
                     /locus_tag="P5335_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRZ73887.1"
                     /translation="MTKILVKQVKSQINCPLTQKKTLVALGLKRLGQVVEHDNSSAIL
                     GMVNKVKHLVSVEESK"
     gene            13255..13707
                     /gene="rplO"
                     /locus_tag="P5335_03030"
     CDS             13255..13707
                     /gene="rplO"
                     /locus_tag="P5335_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRZ73888.1"
                     /translation="MNLSNLQPAEGSTHNQNKRLGRGEGSGKGGTASRGHKGAKSRSG
                     YSKKIGFEGGQMPLQRRVPKFGFKNINRVDYQGVNLDSLQALVDNGVVTDTVDFSVFV
                     DTRLATKNELVKILGRGELKAKLKVTAHKFTATAKAAIEAAGGEAVTL"
     gene            13720..15066
                     /gene="secY"
                     /locus_tag="P5335_03035"
     CDS             13720..15066
                     /gene="secY"
                     /locus_tag="P5335_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRZ73889.1"
                     /translation="MKKFFEALTNVWKIEELKNRILITLGLLLVYRFGAQVTLPGIDA
                     TKLGGLATQTDQGIGSILDMFTGGAFSQASVFALGIMPYISASIVVQLMGIAVPYLQK
                     LQKDGESGRKKINQITRWLTIAITLVQGPGYIYNLYRTLPSEAFLLGFNSFSFLFSAV
                     VILVTGTIFAMWLGEKITDKGIGNGISLLIMVGIIARLPQAFLQEFVSTVKQDNGGLV
                     LLVFEIILWLLVIIASILLVMAVRKIPVQYARRTAGGDFEQDMMGGNRQFIPLKLNAS
                     GVMPIIFAQAIMFIPAAIAGLSKSEAAQSVTGAFSDMFGFWYNFVFALLIVVFTYFYT
                     AITVPTNKMADDLKRSGGFIPGIRPGIETSDFLDRIMSLITLPGSLFLALIAVFPAVV
                     VNLMGVQQNWGMFFGGTSLLIMVGVAIDTMQQINSYLLNRHYDGLMKSGKNRKAVA"
     gene            15072..15287
                     /gene="infA"
                     /locus_tag="P5335_03040"
     CDS             15072..15287
                     /gene="infA"
                     /locus_tag="P5335_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRZ73890.1"
                     /translation="MAKQSAIEQDGSIIEALSNAMFRVELENGHVVIAHISGKMRMHY
                     IKLLPGDKVKLEMSPYDLTKARITYRY"
     gene            15299..15415
                     /gene="ykgO"
                     /locus_tag="P5335_03045"
     CDS             15299..15415
                     /gene="ykgO"
                     /locus_tag="P5335_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HRZ73891.1"
                     /translation="MKVRASVKKRSAECVIVRRKGRLYVINKKNPRFKQRQG"
     gene            15418..15792
                     /gene="rpsM"
                     /locus_tag="P5335_03050"
     CDS             15418..15792
                     /gene="rpsM"
                     /locus_tag="P5335_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRZ73892.1"
                     /translation="MARIAGVDIPKNKRGVIALTYIFGIGKSRSIEILEKAQVSQDKK
                     VQDWNDDEIGAIRDAVSYYKIEGELRSEVSLHIKRLMDIGCYRGIRHRSGLPLRGQRT
                     KNNSRTRKGKRKTVANKKKATK"
     gene            15801..16184
                     /gene="rpsK"
                     /locus_tag="P5335_03055"
     CDS             15801..16184
                     /gene="rpsK"
                     /locus_tag="P5335_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRZ73893.1"
                     /translation="MAKATAKKRKVIVESTGEAHVSATFNNIIISLTNKKGEVISWSS
                     AGKMGFRGSKKNTPYAAQMAAEDCSKVALEAGLKKVKVYVKGPGNGRESAIRSLHNSG
                     IEVTEIIDVTPMPHNGCRPPKRRRV"
     gene            16302..16907
                     /gene="rpsD"
                     /locus_tag="P5335_03060"
     CDS             16302..16907
                     /gene="rpsD"
                     /locus_tag="P5335_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRZ73894.1"
                     /translation="MARYTGPKTKIARKFGEAIFGEDKAFEKRNYPPGQHGLAKRRGK
                     KSEYAVQLMEKQKAKYTYGILEKQFRNLFEKAAASKGVTGEILLQLCEARLDNVVYRM
                     GVAPSRRAARQIVGHRHITVNGEIVNVPSYHLKPGDKVAIREKSKSVEAINSSLANAS
                     KVYEWITWNNDTKEGTFVTVPQRLQIPENIKEQLIVELYNK"
     gene            16929..17921
                     /locus_tag="P5335_03065"
     CDS             16929..17921
                     /locus_tag="P5335_03065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRZ73895.1"
                     /translation="MAIFNFQKPDKVIMIDSTDFEGKFEFRPLEPGYGLTVGNALRRV
                     LLSALEGYAITSVRIEGVDHEFSTISGVVEDVTEIILNLKQVRFKRQIEDVDNETVTI
                     SLSGKDQITAGDFQKFISGFQVLNPELIICNLDAKVHLNMELTIEKGRGYVPAEENKK
                     QNAAIGTIFTDSIFTPIKNVKYAIENFRVEQKTDYEKLVFEIKTDGSINPKDALTEAA
                     KVLIHHFMLFSDERITLEADEIAQTESYDEESLHMRQLLKTKLVDMDLSVRALNCLKA
                     AEVDTLGDLVSFNKNDLMKFRNFGKKSLTELDELVAIKNLHFGMDLSKYKLDKE"
     gene            17960..18466
                     /gene="rplQ"
                     /locus_tag="P5335_03070"
     CDS             17960..18466
                     /gene="rplQ"
                     /locus_tag="P5335_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRZ73896.1"
                     /translation="MRHGKKVNHLSRQQGHRRSLLANMACSLIEHKRINTTVAKAKAL
                     KQFVEPLITKSKEDTTHNRRIVFAYLRNKYGVTELFREVAAKVGDRPGGYTRIIKLGN
                     RLGDNADMAMIELVDFNELYNGGKKEEKKAKSRRGGKAKKAEVTAPESKAPEAPAADA
                     SETTEAAE"
     gene            18625..19296
                     /locus_tag="P5335_03075"
     CDS             18625..19296
                     /locus_tag="P5335_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73897.1"
                     /translation="MRIFSVLFCLVGIVSFAQTDVIENSNFQTNSSNMTYGTGAWAKS
                     KPLDTSIQGSEYLFPSWDGNFLVTSKQGNQYKLFNLNYNLNTKKLETVMGKDSVFQYD
                     VSQIDYIKSFNRKYKAINNEFYQELAQGDKISFLKGFYVSVMDGVVNPLTQQYTTPNR
                     YVQKNKYFYLKGEQFQEIKLKKSDVLKVFDDKAEVIKKYAKEKNLSFGDENDVASLFR
                     YYNTL"
     gene            19361..20479
                     /gene="carA"
                     /locus_tag="P5335_03080"
     CDS             19361..20479
                     /gene="carA"
                     /locus_tag="P5335_03080"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRZ73898.1"
                     /translation="MNYTTRKPAILLLADGTLFHGKSIGIEGITFGEVCFNTGMTGYQ
                     EIFTDPSYFGQIMVATNAHIGNYGVNDQEIESDSIKIAGLVCKNFSFNYSRDDASGSL
                     YDYFEKQNLIAISDVDTRALVSYIRDNGAQNAVICTNGTTIQELKLQLANVPDMKGLE
                     LASKVSTKEPYFFGNENATYKIAALDLGIKKNILRNLAKRDCYIKVFPFDSTFDDLKG
                     FNPDGYFLSNGPGDPEPLAGAIEVAKEIIAINKPLFGICLGHQVIGLANGVSTYKMFN
                     GHRGINHPVKNLMTGKGEITSQNHGFAVVREELEKNPDLEITHEHLNDGTVAGMRMKS
                     KNCFSVQYHPEASPGPHDSSYLFDDFIDIIQKSKLQEV"
     gene            20640..21932
                     /gene="eno"
                     /locus_tag="P5335_03085"
     CDS             20640..21932
                     /gene="eno"
                     /locus_tag="P5335_03085"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRZ73899.1"
                     /translation="MSIIIKVHARQILDSRGNPTIEVDVITENGILGRAAVPSGASTG
                     EHEAVELRDGGKSYMGKGVLKAVDNVNTTIADEIVGMSVFEQNQIDKTMIELDGTPNK
                     ANLGANAILGVSLAVAKAAANELGMPLYRYVGGVSANTLPVPMMNIINGGSHSDAPIA
                     FQEFMIMPVKATSFTHAMQMGTEIFHNLKKVLHNRHLSTAVGDEGGFAPNLAGGTEDA
                     IESIKKAVENAGYAFGEDVMIALDCAASEFYVDGKYDYKKFEGETGKIRTSAEQAEYL
                     ADLASKYPIISIEDGMEENDWEGWKYLTDLIGNKIQLVGDDLFVTNVERLKTGIDKGI
                     ANSILVKVNQIGTLTETIAAVNMAHNAGYTSVMSHRSGETEDNTIADLAVALNCGQIK
                     TGSASRSDRMAKYNQLLRIEEELGNTAYFPGKKAFKQN"
     gene            22055..23392
                     /locus_tag="P5335_03090"
     CDS             22055..23392
                     /locus_tag="P5335_03090"
                     /inference="COORDINATES: protein motif:HMM:NF020677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HRZ73900.1"
                     /translation="MKKNYFAILSFIVFVFNSNAQVTIPIYDNVLFYDGYASLVNYPT
                     EPDVIRLRNDLFTKKLTPEVLAQIGTSLTLNISISAACDNYDRIGNINLALVPKGATS
                     YNPDTTNRLELGRYITPFMNKNVPPNLVPYTFSVDNITKILKDTYLNTIYDFWIEFEL
                     FGVPYAAQTQISGCSGRNDVFYGTLNFITNTETSSPNNTYIHTLNFKKILNDYQAGAS
                     DGIFQTVRTIYFDLPSAINNAKFYLITSSHGANAGGEEYNRRWHYISLNSNQILSYLP
                     GEPTCEPYRVFNTQGNGIYGPTPRTPEEWQSFSNWCPGAVIPIREMDLGNLAAGNHMF
                     RIAVPTAVFVGNEGYIPVSLYLQGENTALNVDDFEAIIVDHYPNPTKNKVFISCRKII
                     DAIEIVDLQGKIIESKVVENTETEVDFTQFSNGVYFIKMKLGEVTKTHKIIKE"
BASE COUNT         8529 a         3401 c         4565 g         6977 t
ORIGIN      
        1 atttgttttg ctgaaaatat ctagtacatt tagattgata atttattaaa atttattgtt
       61 tttcacgaga gtgtttcaat atattgtgca aaaactaatc ctttatgaaa acaacactaa
      121 aaacagtact acttttattt tttaccacta gttgtttttc acaaatctat tattccaatt
      181 atttagatga aacgtctgaa tggagagaaa tcagtaaaat ggttaatcag gcaggagcat
      241 attatttcac cttttcaact cgattttttg agggttttga gaacattaat ggatttactt
      301 attataaaat gtataaaacc aaatatacta aaggatacta tattggtgat tattcccctt
      361 tttttatttc tgatgaagag acttctttcc tgggatttgt aagggaagat aatagcggaa
      421 aattttatac cggaacaagt aatggaaatg aaaatttgtt tttcgaaaat caattagtga
      481 tagatgctca gattggggat tcgttttcaa ctcttaatgt ttatagtgct tttggtcaag
      541 aatcctcttg tacaattgaa aatatctcat ttattagtct gtctggtatg aatctaaaag
      601 ttgttagtga tgaaaatgaa aattcattaa ttaaaggagc aacggtaatt gagggaattg
      661 gtcgtgttta tccatattgt gaatcccctc aaattttaga taacgttatc agtaatgatc
      721 aagagaggat tttttgttat accaaacaag gtcaaattta tcaatatgaa aatgattata
      781 atttatttga tatggtatta aattgtgatt cttttcctaa tccaaatcgt cagggattaa
      841 gtgtttcaaa ttttaacaca aatgctattc aaatatttcc taatcctaca acttctacca
      901 taaatattaa gataaacaag gagaatatta tttcaattca actttttgat gtagaaggaa
      961 gggttttgga agatataaac ggtaatgaat catcatttca attcgatttg tcaaattatc
     1021 gaaatgggac ttatttagta aaagtaataa ctgaaagagg tagtaaagta gtaaaaattt
     1081 taaaaatttg attttggtat aaaattttcg caatagtttc aaagcaagag ccattagaat
     1141 atattataca ttctaatggt tttgtttttt aataaaaagt aattcctaaa aggtaagtcg
     1201 tttagctgta aatagtcata attaagtttg tgtttgaaaa taaataaatt atgatttgtt
     1261 ttgctaaaaa tatttactac atttgcaacc ccttaaaata gggtaatttt aaaaacatag
     1321 aaatttttag tattaattat gccaacaatt caacaattag taagaacagg gagagcccaa
     1381 ataactaaga agagtaaatc ggctgcttta gattcttgtc ctcaaagaag aggtgtgtgt
     1441 actcgtgttt acacaacaac tcctaaaaaa ccaaactcag caatgagaaa ggtggctagg
     1501 gttcgtttaa caaacggtaa cgaggtgaat gcatatattc ccggagaagg acacaatcta
     1561 caagagcact cgatagtatt agttagaggc ggaagggtta aagatttacc aggagtacga
     1621 taccatatcg ttcgaggtgc gttagatact gcaggtgttg caggtcgtac acaacgtcgt
     1681 tcaaaatatg gagcaaaacg tccaaaacca ggtcaagtag cagcacccgt taagaagaaa
     1741 aagtaagtta aaaactttta aagaaaagac atgagaaaaa gacaggccaa aaaaagacct
     1801 cttttaccgg atccaaaatt caatgatcaa ttggttacac gttttgtgaa caacttaatg
     1861 tgggatggta aaaaatcaac agctttcaaa gtattctatg atgctttaga aattgttgat
     1921 gcaaaaaaac aagatgcaga aaaatcttca ttggaaacat ggaaagacgc tttaacaaac
     1981 gttatgcctc acgtagaagt acgtagtcgt agagtaggtg gagctacctt tcaaattcca
     2041 atgcaaattc gtccggatag aaaaatttct tacgctatca aatggatgat tttatatgca
     2101 agaagaagaa acgaaaaatc aatggcagcc aagttagctt cagaaatttt agcagcagct
     2161 aaagaagaag gtgcagcggt taagaaaaga atggatacgc acaaaatggc agaagctaat
     2221 aaagctttct ctcactttag attttaattc ataagaaatg gctagagatt taaaatacac
     2281 aagaaatata ggaattgctg ctcacattga tgctggtaaa acaacaacaa cagagcgaat
     2341 ccttttttat acaggaaaat cacataaaat tggtgaagtg cacgatggtg ctgcaacaat
     2401 ggactggatg gcacaagagc aagaaagagg tattactatt acttctgctg ctacaacttg
     2461 tgaatggaat ttcccaactg aacaaggaaa agttttacct gaaacattac cttatcactt
     2521 taacattatc gatactcccg ggcacgtgga ttttaccgtt gaggtaaatc gttcgttacg
     2581 tgtattggat ggtttagttt tcttatttag tgcagtagat ggtgttgagc ctcaatctga
     2641 aactaactgg agacttgctg atcaatatcg tgttccaaga atgggatttg ttaacaaaat
     2701 ggaccgtcaa ggttcaaact tcttaggagt ttgtcaacaa gttagagata tgttaaaatc
     2761 taatgcggtt gcaatcacat tgccaatcgg agaagaaatt gactttaaag gtgtagtgga
     2821 tttagttaaa aaccaagcta ttgtttggca tgacgaaaca ctgggagcta catttgatgt
     2881 tgtagatatt cccgctgata tggtggatga agtaaaacaa taccgttcaa ttttgattga
     2941 agcggtggcg gattatgacg aaaacctttt agacaagtat atggaggacg aaaactctat
     3001 aactgaagaa gaaattaata atgctttacg tgctgctact atggatatgg ctatcattcc
     3061 tatgattgcc ggatcttctt tcaaaaacaa aggagttcaa ttcatgttag atgccgtatg
     3121 taaatattta ccttcaccaa tggataaaga gggaattgtg ggtattcacc ctgatgacca
     3181 agatttgttg gaagaagatc aaagaaaaat tttacgtaaa ccggatgtta aagagccgtt
     3241 tgctgcttta gcatttaaaa ttgctaccga cccttatgtt ggtcgtttgg ctttcttccg
     3301 tgcttactca ggtcgtttag atgctggttc ttatgtattg aacacccgtt cagacaataa
     3361 agaacgtatt tctcgtattt atcaaatgca cgcaaacaag caaaacccaa tcgattatat
     3421 cgaagcaggt gatattggag ctgctgtagg atttaaggat atcaaaacag gagatacatt
     3481 gtgtgatgaa aaacacccaa ttattcttga gtcaatgaaa ttccctgatc cggtaattgg
     3541 tattgcgatt gagcctaaaa caaaagctga cgttgataaa atgggtatgg ctttggctaa
     3601 attagcggaa gaagatccta cgtttacggt tagaacagat gaagcttccg gacaaacgat
     3661 tatttcaggt atgggtgagt tgcacttaga cattcttgta gatcgtatga aacgtgaatt
     3721 caaagtggaa gtaaaccaag gtgaacctca agttgaatac aaagaagctt tcactaaagc
     3781 agcacaacac agagagactt ataaaaagca atctggtgga cgtggtaaat ttggtgatat
     3841 cgtatttaaa cttggaccag ctgacgacgt agacggaaaa ccatatgttg gtcttcagtt
     3901 tgtgaatgaa gttaaaggtg gtaacgttcc taaagaatat attccatcag ttgaaaaagg
     3961 tttcaaagaa gctatgaaaa caggtccatt agctggatat gcagtagata gtttaaaagt
     4021 aactttatta gacggatctt tccatcctgt ggattcagat gcactttctt tcgaattggc
     4081 tgctaaaatg ggttacaaag aggttgcaaa agctgccgga gctgtaattc ttgagccaat
     4141 catgaaaatt gaagtgatta cacctgaaga aaacatgggt gatattgttg gagatttaaa
     4201 tcgtcgtaga ggtcaagtaa atgacatggg agatagagcg ggtgcaaaaa cgattaaagc
     4261 gtttgtacca ctttctgaaa tgtttggata tgtaactact ttaagaacat tgtcttctgg
     4321 tagagcaact tctacaatgg aattttcaca ttatgaacaa actccttcaa acatttctga
     4381 agaagttatc aaaaaagcta aaggaaacgc ttaattctaa cgaaaatgag tcaaaaaatc
     4441 agaataaaat taaaatctta cgatcatatg ttggttgata aatcagctga gaaaatcgta
     4501 aaaactgtaa aaagtacagg tgcagtggtt accggaccaa ttccgttacc aactcacaaa
     4561 aaaatattca ctgtactacg ttctccacac gttaacaaaa aatcaagaga acagtttgaa
     4621 gtgagttcat acaaaagatt gttagatatc tacagttctt cttcaaaaac cattgacgct
     4681 ctaatgaaat tagagttacc tagtggtgtt gaagttgaaa tcaaagtgtg ataatgtaca
     4741 aagatgaaag tattaagtag taagatattc tcttactgct taatattctt gtcttaatac
     4801 aaaacaaaaa ataagaattt ttaatcattt ataaatagtt taatatgtct gggttaattg
     4861 gaaaaaaaat cggcatgact agcattttcg atgaaaacgg gaagaatatt ccttgtacag
     4921 taatcgaagc tggcccctgt gtcgttaccc aagtcagaac caaagcggtt gacgggtatg
     4981 aagcgtttca gcttggtttc gatgacaaaa cagaaaaaca cgctactaaa gctgacttag
     5041 gtcactttaa aaaagcggga acttctgcta agaaaaaagt cgttgaattc caagaatttg
     5101 aaacggagta taaattaggt gatttaatca ctgtagatct attcagcgaa ggagaatttg
     5161 ttgatgtaca aggtgtatca aaaggtaaag gtttccaagg ggttgttaaa cgtcacggtt
     5221 ttggtggtgt tggacaagct actcacggtc aacataaccg attaagagca ccaggttctg
     5281 taggagcttc ttcttatcca tctagagtat tcaaaggaat gcgtatggct ggaagaatgg
     5341 gaggagataa tgtaacaatt caaaacctta gagttttaaa agtagtagcc gataaaaatc
     5401 tacttcttgt taaaggatgt gttcctggac acaaaaactc ttatgtaatc attcagaagt
     5461 aatggaagta aaagttttag atattaacgg aaaagaaaca ggtagaaaag ttcaactttc
     5521 tgactctgtt ttcgctatcg agcctaacaa tcatgctatc tatcttgatg tgaaacaata
     5581 tcttgctaat caaagacaag gtactcacaa agcaaaagaa agagctgaag taacaggaag
     5641 tactcgtaag attaaaaaac aaaaaggtac tggtactgcc cgtgccggta gtattaagaa
     5701 tcctttgttc aaaggaggag gtacagtttt cggtcctaga ccaagaagct attctttcaa
     5761 attgaacaaa actttaaaaa gattagctcg taaatcagct ttctctcaaa aagtaaaaga
     5821 ctctgaatta gtagtagtag aagatttcaa tttcgacact ccaagtacta agaattttat
     5881 tacagttctg aaagccttag gtttagaaaa taaaaaatcg ctatttgtgt tgggtgattc
     5941 aaataaaaat gtatatttgt cgtcacgcaa tttaaagggc tcaagagttg taactaactc
     6001 agaattaagt acttatgcta ttttaaacac aaataaatta gtacttatgg aaagttcttt
     6061 agaaggaatt gaagaaaatt taagtaaata ataggccatg agtatcataa ttaaacctat
     6121 cataactgaa aaaatcacga agcaaagtga gttaaacaat cgttatggtt ttgtagtgga
     6181 tagaaaagca aacaaaattc aaatcagaaa tgcggtggaa gctgcttatg gtgtgaaaat
     6241 tgtagctatc aacactatga atgttagacc agacagatct gtgaaataca ctaaaagtgg
     6301 aatgataagt gctaaatcaa atgcttacaa aaaagcaatt gtagaagtac aagaaggaga
     6361 aacaattgat ttttataaca atatctaagt aagaaatgtc agttagaaaa ttaaaaccta
     6421 ttaccccggg tcagcgtttt agagttgtga atggttttga cgccattaca actgataagc
     6481 cggaaaagtc attattggca ccgaaaaata actctggagg tagaaatagt caaggaaaaa
     6541 tgaccatgcg ttatacaggc ggtggtcaca aacaaaagta tcgtgtaatt gattttaaaa
     6601 gaactaaagc tggaattcct gctacagtta aatctattga gtacgatcca aacagaactg
     6661 catttattgc cttattgagt tatgtagatg gagctaaaac ttacatcatc gctcaaaatg
     6721 gattaaatgt tggtcaaaca gttacttcag gtgaaggagc agctccggaa attggaaatg
     6781 cactaccatt aagtaaaatt ccattaggaa cagtaatttc ttgtattgag ttaagacctg
     6841 gtcaaggagc tattattgct cgttctgcag gaacatttgc tcaattaatg gcaagagatg
     6901 gaaaatacgc cactatcaaa atgccttctg gtgaaactag attaatcttg ttaacttgta
     6961 tggcaactat tggtgcagta tctaattcag atcaccaatt aattgtttcc ggtaaagcgg
     7021 gtagatcaag atggttgggt agaagaccta gaacaagacc agttgcaatg aaccctgtag
     7081 atcacccaat gggtggtggt gaaggtcgtt cttctggagg acacccacgc tctagaaacg
     7141 gaattcctgc taaaggatat agaactcgtt ctaaagtgag cccaagtaat aagtatattg
     7201 ttgaacgcag aaagaaataa ttagatagac atggcacgtt cattaaaaaa aggacctttc
     7261 gtacattata aattagataa gaaagtactg gaaaacattg agaaatcaag caaagcagtg
     7321 atcaaaactt ggtcaagagc ttcaatgata actcctgatt ttgtaggaca aactatcgca
     7381 gtacacaatg gtcgtcagtt tgtacctgtt tatgtaacag aaaacatggt aggacacaag
     7441 ttaggagaat tttcaccaac aagatcattt agaggtcatg ccggtgctaa aaataaaggt
     7501 aaaaaataag aagcaatggg agttcgtaaa agagaaacag cagacgcgag aaaagaagct
     7561 aataagcaaa ttgctttcgc aaagttaaat aactgcccta cttcacctag aaaaatgcgc
     7621 ttggttgcag acttggtaag aggtcagaaa gtggaaagag cactaaatat tttaagattt
     7681 agtcaaaaag aagcttcaag aaaattagaa aaattgttgt tatcagctat cgctaattgg
     7741 caagccaaaa acgcagaagc taatattgaa gaggctgctt tattcgtaaa agagatcaga
     7801 gttgatggtg gaatgatgtt aaaaagactt cgtccagctc ctcaaggtcg tgcacacaga
     7861 attagaaaac gttcaaatca cgttacaatc gtgcttggag ctagtaataa cacacaaagc
     7921 aattaataaa gatgggacaa aagacaaatc caattggaaa cagacttggt atcatcagag
     7981 gatgggattc aaactggtat ggtggaaatg attacggtga taaaatcgct gaagattata
     8041 aaatcagaaa gtatattcat gctcgtttat caaaagctag tgtatcaaaa gtaatcatcg
     8101 agagaacttt aaaacttgta accgttacta tcactactgc tagacctggt atcattatcg
     8161 gaaaaggtgg acaagaggta gacaagttga aagaagaact taagaaaatt actgacaaag
     8221 aggttcaaat taacatcttt gaaattaaaa gacctgagat tgatgcttat ttagttgcaa
     8281 caagtatcgc tcgtcaaatc gaaagccgta tttcatacag aagagctatt aaaatggcta
     8341 tcgcagcagc aatgcgtatg aatgcagaag gtgtaaaggt tttgatttcc ggtcgtttga
     8401 atggagctga aatggcacgt tctgaaggtt tcaaagaagg tagaattcct ctatcaactt
     8461 tcagagccga tattgattat gctttggcag aagctcacac tacttatggt agaatgggaa
     8521 tcaaagtatg gatcatgaaa ggtgaagttt acggaaagag agatctttct ccattagttg
     8581 gcatggataa aaaacaagct ggcggtggaa gtaaaggtgg tgatgctcca agaggaggaa
     8641 aaccaaacgg aaaatcaaac ccacgtaaaa gaaagtaatt ttttaaatta aagaaaaatg
     8701 ttacagccta aaagaacaaa ataccgtaag gtacaaaaag gtagaatgaa aggtgattct
     8761 caaagaggtc acgaactttc taacgggatg tttggaatta aatcaatgga ttcatcattt
     8821 ttaacttcac gtcaaatcga agctgcacgt attgctgcaa ctcgttatat gaaaagagaa
     8881 ggacaattat ggattaaaat tttcccagac aaaccaatca ctaagaaacc tcttgaagta
     8941 cgtatgggta aaggtaaagg tgcagttgaa tattgggctg ccgttgttaa acccggaaga
     9001 attatgtttg aagtaggagg agtacctttg tcagttgcaa aagaagcatt acgcttagca
     9061 gctcaaaaac ttcctgttaa aactaagttt gttgtcgcta gagattttga agcataatcc
     9121 tatttaatta tgaaacaatc agaaattata agtctatctt cagctgagtt acaagagaaa
     9181 cttagtcagt caagaaaaac gtatgccgac ctaaaaacga ctcatactat ttctccaatt
     9241 caaaacccac ttcaaattaa agcggtgcgt agaacaattg cgagattagt aactgagcta
     9301 agtaaaagag agttacaata attgtattct gctgaaagat ggaaaaaaga aatttaagaa
     9361 aagaaagagt tggtgttgtt actagcaaca gaatggagaa atctatcgtt gtttctgaaa
     9421 caagaaaagt aaaacaccca ttatacggta agttcgtgtt gaaaactaag aaatatgttg
     9481 cacacgacga aacaaacgac tgtaatattg gagatactgt aagaattagc gaaacgcgtc
     9541 ctttaagtaa atcaaaatgt tggagattag ttgaaatcat tgaaagagcg aagtaattat
     9601 ggtacaacag gaatctagat taaaagtagc agataacaca ggagcaaaag aagttttgac
     9661 tatccgtgtt ttaggaggaa cgaaacgtcg ttatgcctct gttggagata aaattgtagt
     9721 ttcaattaaa gatgcaaccc caaacggaaa cgtaaaaaaa ggagctgtat caactgcagt
     9781 tgttgtacgt accaaaaaag aagtgagaag agctgatggt tcttatattc gttttgatga
     9841 caatgcatgt gttcttttga atgcggcagg agaaatgaga ggaactcgtg tttttggtcc
     9901 agtagcaaga gaacttcgtg aaaaacaatt catgaaaatt gtatcattag cacctgaagt
     9961 gctttaaatc gattgatgat gacaaagtta aaaattaaat caggagacat tgtaagagtt
    10021 attgcaggtg accataaagg tgctgaaggt aaagttttac gtgtttttat agatgcaaac
    10081 aaagcgattg ttgaaggtac taacatggtt tcagttcata ctaaaccaag tgctaaaaac
    10141 cctcaaggtg gaatcgtaaa gaaagaagct cctattcaag tatcgaactt gtctttaatt
    10201 gacccaaagt cgaaaaaagc aactaaagtt ggattcaaag tagaaggaga taagaaagta
    10261 agattttcaa aaaaatctaa tcaagtatta tagtgatggc ttatatacct agactaaaag
    10321 aagaatataa aagtagagtt atccctgctt tgaaagaaga attcggttat aaaaacgtaa
    10381 tgcaagttcc taaattggaa aaaatcgttt taagccgtgg tgtcggagct gcagtatcag
    10441 acaaaaaatt ggttgattat gcagtagacg agttgacaaa aatcaccgga caaaaagctt
    10501 tagctactat ttctaaaaaa gacgttgcgt cttttaaact aagaaaaggt atgccaattg
    10561 gtgctaaagt aactttacga ggagaaagaa tgtatgaatt cttagataga ttgattactt
    10621 cttctttacc acgtgtaaga gattttggtg gtatcaaagc aaccggtttt gacggaagag
    10681 gtaattataa ccttggagtt ttggaacaaa tcattttccc agaaattgat attgataaag
    10741 taaacaaaat ttcaggtatg gatattactt ttgtaacaac tgccaaaaca gataaggaag
    10801 caaaatcatt attagcagaa ttaggattac cttttaaaaa gaattaagtt atggctaaag
    10861 aatcaatgaa agcccgtgag gttaagagac aaaaaacggt agaaaagtac gctgaaaaaa
    10921 gaaaggcttt attagaagcc ggagattatg aaggtttaca aaaattacct aaaaatgctt
    10981 cgccagttcg tttacacaac cgttgtaaat taacaggaag accaagaggg tatatgcgtc
    11041 aattcggtct ttcacgtgta acattccgtg aaatggctaa ccaaggttta attccaggtg
    11101 tcaaaaaagc tagttggtaa aaacaaattt taattggttt caggttcaat agaaagagtt
    11161 tttgttgaaa accgttaccg caaatttata catatgtata cagatcctat tgcagattat
    11221 ctaactagag ttagaaatgc agtgaaagca aatcacaaag tggttgaaat tccggcatct
    11281 aaccttaaaa aagaaatcac aaagatttta tttgatcaag ggtatatttt aagttacaaa
    11341 tttgatgaca cttctgttca aggaagcatc aaaattgctc ttaagtacga caaagttact
    11401 aaagagcctg taattagaga tatccaaaga attagtaaac caggtttacg taaatatgca
    11461 ggttcttcaa accttccaag aatccttaat ggattaggaa ttgcaattgt ttcaacttca
    11521 aaaggtctta tgactggaaa acaagctaaa caattgaatg taggtggaga agtaatttgt
    11581 tacgtatact aataaaaaga ctgaacgatg tcaagaatag gtaaaaatcc aattgtgatt
    11641 ccagcaggtg taacacttga agttaacgaa ggtgtaatca cagttaaagg gaaattaggt
    11701 caacttacac aggagttttc ggacgttact gtaactgttg aagacggtca agtgttagta
    11761 gaaagatctt ctgaccataa agaccaaaga gcaaagcatg gtttgtacag atctttaatc
    11821 agtaatatgg ttaatggtgt atctaccggt tttacaaaag aattagagtt agttggtgta
    11881 ggatatagag catctaacca aggtcaaaaa ttagatcttg cattaggttt ttctcataat
    11941 atcgttttag aagttgctcc ggaagtaaaa ttggagacaa tttcagaaaa aggtaaaaat
    12001 ccaatcgtaa aattaacctc tcacgataaa caacttttag gacaggtagc cgctaaaatt
    12061 agaggtttcc gtagaccaga accttacaaa ggaaaaggtg tgaaattcgt aggtgaagta
    12121 ttaagaagaa aagcaggtaa atcagcttaa aaattaagat tatgtcatta acaaaatctg
    12181 atagaagaca gagaattaag ttcagaatta gaaagattgt aagcggaact gctactaaac
    12241 caagactttc tgtattcaga agcaacaaag aaatctatgc tcaattaata gatgacttaa
    12301 atggagttac tttattagca gcatcctcta gagataaagg agtagacgct aaaggaacaa
    12361 acattgaagt tgcatcagca gtaggtaaat tagttgctga aaaagcattg aaagctggta
    12421 ttgatacagt aactttcgat agaggaggat acttatacca cggtcgtatt aaatcattag
    12481 cagatggagc gagagctgcc ggacttaaat tctaagaagt tatgtataac aaatacaaaa
    12541 atgtagagtt agtaaaacct agtggtcttg aattaaaaga tcgtttggtg agtgtaaatc
    12601 gtgttaccaa agtaacaaaa ggtggtagag catttggttt ttctgctatt gttgtggtag
    12661 gtgatgaaaa cggagtagtt ggtcatggat taggaaaatc taaagacgta tctgaagcta
    12721 ttgcaaaagc agtggaagat gctaagaaaa atttagtaaa aattccttta agtggtcaat
    12781 ctgttcctca cgaacaaaaa ggtaaatttg gtggagcacg tgtattctta atgcctgcat
    12841 cacatggtac aggagttatt gctggtggtg ctgttcgttc agtacttgaa tcagttggaa
    12901 ttcacgatgt attatcaaaa tctcaaggat cttcaaaccc acataatgtg gtaaaagcaa
    12961 ctttcgatgc attacttcaa atgagaagtg catatactgt agctaaacaa agaggaattt
    13021 ctttagagaa agtttttaaa ggttaatatt caaggaaatt atgacaaaga ttttagtaaa
    13081 acaagtaaaa agtcaaatta actgtcctct tacacaaaag aagacgttag ttgctttggg
    13141 tctgaaaaga ttaggacaag ttgtagagca tgataattca tctgctatcc ttggaatggt
    13201 aaataaagta aaacacttag tttctgtaga agaatctaaa taacactatt caaaatgaat
    13261 ttaagtaact tacaaccagc tgaagggtca acacataatc aaaataaaag attaggtaga
    13321 ggagaaggtt ctggaaaagg tggtactgca tcacgtggtc acaaaggagc aaaatctcgt
    13381 tctggttatt ctaagaagat tggttttgaa ggagggcaaa tgccacttca aagacgtgtg
    13441 cctaagtttg gatttaaaaa tatcaataga gtagattatc aaggggtaaa ccttgattct
    13501 ttacaagctt tagtagataa tggtgtagtg actgatactg tagatttttc agtattcgtt
    13561 gatactcgtt tggctactaa aaacgaattg gtaaaaatat taggtagagg tgaattaaaa
    13621 gccaaattaa aagtaaccgc tcacaaattt actgctactg caaaagctgc tatcgaagct
    13681 gcagggggtg aagctgtaac tttataattt actaactgta tgaagaaatt ttttgaagca
    13741 ctcacaaacg tttggaaaat agaagaatta aaaaacagaa ttttaattac tctaggttta
    13801 cttcttgttt atcgttttgg agcacaggta actttaccag gtatagatgc aactaaatta
    13861 ggtggtttag ctactcaaac agatcaggga attggttcaa ttctagatat gtttactgga
    13921 ggggcttttt ctcaagcatc tgtattcgct ttaggtatta tgccatacat ttctgcatcc
    13981 attgtggtgc aattaatggg aatagcagtt ccatatttac aaaaactgca aaaagatggt
    14041 gagagtggaa gaaaaaaaat taatcaaatt acacgttggt taactatagc aatcacttta
    14101 gttcaaggtc ctggttatat ttataactta tacagaactt taccatcaga agcatttttg
    14161 ttaggattta attccttttc tttcttattt tcagcagtag ttatcttggt aacaggaact
    14221 atttttgcaa tgtggttagg agagaaaatt actgataaag gtatagggaa tggaatttcc
    14281 ttattgatta tggtaggtat tattgctcgt ttacctcaag cttttttaca agagttcgtc
    14341 tcaactgtaa aacaagataa tggtggatta gtacttttag tatttgaaat tattctttgg
    14401 ttactagtga ttattgcaag tatcctttta gtgatggcag tacgaaaaat acctgttcaa
    14461 tatgccagaa gaactgcagg cggtgatttt gaacaagata tgatgggcgg taatagacag
    14521 tttataccat taaagcttaa tgcttctgga gtaatgccaa ttatctttgc tcaagctatc
    14581 atgtttattc cggcagcaat tgcgggttta tcaaaatctg aagcggctca atccgttaca
    14641 ggtgccttta gtgatatgtt tggtttttgg tataattttg tatttgcttt attgattgtg
    14701 gtatttacct atttctatac agctattaca gtgccaacaa acaaaatggc tgatgattta
    14761 aaaagaagtg gaggattcat accgggaatc agaccaggta ttgaaacatc tgacttttta
    14821 gatagaataa tgtccttaat aacattaccg ggttctcttt tcttagcact tatagcagta
    14881 tttcctgccg tagttgtaaa tttgatggga gttcagcaaa attggggtat gtttttcgga
    14941 ggaacatcgc tattaattat ggtaggtgtt gcaattgata ctatgcaaca aataaattct
    15001 tatttgctga atagacatta tgatggattg atgaaaagtg gtaaaaatag aaaagcagta
    15061 gcttaatttt tatggcaaaa caatcagcaa tagaacaaga cggatcaatt attgaagcat
    15121 tatcaaatgc aatgttccgt gtagaattag aaaatggtca tgtagtgatt gctcatattt
    15181 ccggtaaaat gcgaatgcat tatatcaaat tattacctgg tgataaagtg aaattggaaa
    15241 tgagccctta cgacctgaca aaagcaagaa ttacttatag atattaaagc tattcacaat
    15301 gaaagtaaga gcatcagtaa agaaaagaag tgccgaatgc gtaattgttc gcagaaaagg
    15361 cagattgtac gttattaaca aaaagaatcc tagatttaaa caaagacaag gataattatg
    15421 gcaagaatcg caggggtaga tatacctaaa aacaaaagag gagttattgc tttaacctat
    15481 attttcggta tcggaaaaag cagatctatt gagattttag aaaaagctca agttagtcaa
    15541 gacaagaaag ttcaagattg gaatgatgac gaaatcggag caatccgtga tgccgtttct
    15601 tattacaaaa ttgaaggaga attgcgttca gaagtttcac ttcacatcaa acgtcttatg
    15661 gacattggat gttacagagg aattcgtcat agatctggtc ttccattaag aggacaaaga
    15721 actaaaaaca actctagaac tagaaaaggt aaaagaaaaa ctgttgctaa caagaaaaaa
    15781 gcaactaaat aataagtaat atggctaaag caacagcaaa aaaacgtaaa gttatcgttg
    15841 aatcaacggg cgaagctcat gttagtgcga cttttaacaa tattattatt tctcttacca
    15901 ataaaaaagg agaagtgatt tcatggtcat ctgccggtaa aatgggtttc agaggatcta
    15961 aaaagaatac accttatgca gctcaaatgg ccgcagaaga ttgttcaaaa gtagcacttg
    16021 aagcaggttt aaagaaagtt aaagtttatg taaaaggacc aggtaacgga cgtgagtctg
    16081 ccatcagatc tttacataac agtggaattg aagttactga aattatcgac gtaactccaa
    16141 tgcctcacaa cggatgtaga cctccaaaaa gaagaagagt ttaattttta ctgaaattat
    16201 ttcagcatca ttataatttt taataagtat aacccaaggt agagtttgat tatcgaagga
    16261 tttgacctga attcataatc actaccttaa aacaatttaa aatggcaaga tatactggtc
    16321 caaaaactaa aatagctcgt aaatttggcg aagctatatt cggagaagat aaagccttcg
    16381 aaaaaagaaa ttaccctcct ggacaacatg gtttggctaa aagaagaggt aaaaaatcag
    16441 aatatgcagt acagttaatg gaaaagcaaa aagcgaagta cacttacggt attcttgaaa
    16501 aacaattcag aaacttattt gaaaaagcag ccgcctctaa aggtgtaaca ggtgaaatcc
    16561 ttttacaatt atgtgaagct agattagata atgtggttta cagaatgggc gtagcacctt
    16621 ctagaagagc agctcgtcaa atcgttggac acagacatat tactgtaaat ggtgaaattg
    16681 tgaatgttcc ttcctaccat ttgaaacctg gtgataaagt agccattcgt gaaaaatcaa
    16741 aatcagttga agcaatcaat agttctttag caaacgctag taaagtatat gagtggatta
    16801 cttggaacaa tgatactaaa gaaggtactt ttgtaactgt tccacaacgt ttacaaattc
    16861 cagaaaacat caaagaacaa ctaatcgttg agttatacaa caaataataa ttgacagaag
    16921 tcgtaattat ggcaatattt aattttcaga agcccgataa agttatcatg atcgattcaa
    16981 ccgattttga aggtaagttt gaattcagac ctttagaacc tggttacgga ttgactgttg
    17041 gtaatgcact aagaagagtt ttactttccg ctcttgaagg atatgcaatt acatctgttc
    17101 gtattgaagg tgtagatcat gagttttcta ccatttccgg tgttgtggaa gatgtaacag
    17161 aaattatttt gaacttaaaa caagttcgtt tcaaacgtca aatcgaagat gttgacaatg
    17221 aaactgttac tatttcgctt tccggtaaag accaaataac tgctggtgat tttcaaaaat
    17281 ttatttcagg tttccaagtt ttgaatcctg aattaattat ctgcaacctt gatgcaaaag
    17341 ttcacttgaa tatggagttg actattgaaa aaggtagagg atatgttcct gcagaagaaa
    17401 ataaaaaaca aaatgcagcc atcggaacaa ttttcaccga ttcaattttt acacctataa
    17461 aaaatgtaaa atatgcaatt gaaaatttcc gtgtagagca aaaaacagat tatgaaaaat
    17521 tagtttttga aattaaaact gatggttcta ttaatccaaa agatgcctta acagaagctg
    17581 caaaagtttt aattcaccac tttatgttgt tctcagatga gagaattaca cttgaagctg
    17641 acgaaatagc tcaaacagaa tcctatgatg aagagtcatt acacatgaga caattgctta
    17701 aaactaagct tgtggatatg gatctttctg taagagcatt aaattgtttg aaagcagcag
    17761 aggttgatac attaggtgat ttagtatcat tcaataaaaa cgacttgatg aaattccgta
    17821 atttcggtaa gaaatcattg accgagcttg atgagcttgt agcaattaaa aacttacatt
    17881 tcggaatgga tttgtcaaaa tataaactag ataaagaata atctaatccg cctttggcga
    17941 gattaaatgt aagatagcga tgagacacgg aaaaaaagta aatcatttaa gtagacaaca
    18001 aggtcataga agatctttgt tagctaatat ggcttgttca cttattgagc acaagcgaat
    18061 taatacaaca gttgctaaag cgaaagcttt aaaacaattt gttgaacctt taataacaaa
    18121 atcaaaagaa gatactacgc acaatagacg tattgtattt gcttacttga gaaataaata
    18181 tggcgttact gaacttttta gagaagtagc agctaaagta ggtgaccgtc caggtggtta
    18241 tactcgtatt atcaagttag gaaatcgttt aggtgacaac gctgatatgg cgatgatcga
    18301 attagtagat ttcaatgaac tttacaacgg aggtaaaaaa gaagagaaaa aagcaaaaag
    18361 ccgccgtggg ggtaaagcta agaaagcaga agttactgct cctgaatcca aagcacctga
    18421 agctcctgct gctgacgcct ctgaaacaac cgaagctgca gaataataaa aaaattcatt
    18481 ttattaaaaa aaggatatac tttatagttt atcctttttt ttatgtttaa taaagttttt
    18541 taccattgtg aaatatttat atattattta acagaaaata gttatttttt aaatgtacct
    18601 ttttatatat tcgcacaaaa aaagatgaga atattcagtg ttttattttg cttagttgga
    18661 attgtatctt ttgctcaaac agatgttata gaaaacagta atttccaaac taattcttct
    18721 aacatgactt acggaacagg tgcttgggct aaaagtaagc ccttagatac ttccattcaa
    18781 gggtcagaat atttatttcc aagttgggat ggtaattttc ttgttacttc taaacaagga
    18841 aatcaatata aattattcaa tttaaactat aatcttaata caaaaaaatt agaaacagta
    18901 atgggtaaag attctgtttt tcaatatgat gtttcacaaa tagactacat taaaagtttc
    18961 aatagaaaat ataaagccat aaataatgag ttttatcaag aattggctca gggagataaa
    19021 atttcctttt taaaaggttt ttatgtctca gttatggatg gagtagttaa tccactaaca
    19081 caacagtata ctacaccaaa tcgttatgtt caaaaaaata aatattttta tttaaaagga
    19141 gagcagtttc aagaaataaa attaaaaaag agtgatgtct taaaagtttt tgatgataaa
    19201 gcagaagtaa taaaaaaata cgctaaagag aaaaatcttt cctttggaga tgaaaatgat
    19261 gtggctagct tgtttaggta ttataataca ttataagaaa aaaaagataa gcattgctta
    19321 tctttttttt tgttttaaat ttgcaaatca acacacaact atgaattata caacacgaaa
    19381 accagctatc ttacttcttg cagacggcac tctatttcat ggcaaatcaa ttggaattga
    19441 aggcattact tttggtgaag tttgtttcaa taccggaatg acaggttatc aggaaatttt
    19501 taccgaccct tcctatttcg gacaaattat ggtagctact aatgctcata ttggaaatta
    19561 tggtgttaat gatcaagaaa tcgaatcaga ttccatcaaa attgccggtt tagtatgtaa
    19621 aaactttagt tttaactatt ctcgtgatga tgcttcagga agtttatatg actattttga
    19681 aaagcaaaat ttaattgcta tttcggatgt agacaccaga gctttggtaa gttatattcg
    19741 tgataatggt gctcaaaatg cggttatatg cacaaacgga actactattc aggaactaaa
    19801 attgcaactt gcaaatgttc ctgacatgaa aggactcgaa cttgcttcta aagtatcaac
    19861 aaaagaaccc tacttttttg gcaatgaaaa tgctacctat aaaattgctg ctttagattt
    19921 aggaattaaa aaaaatatac ttagaaattt agcaaaaaga gattgctaca taaaagtttt
    19981 tccttttgat tcaacttttg atgacttaaa agggttcaat cctgatggtt actttttatc
    20041 taacggaccc ggagatcctg aaccgctggc cggtgctatt gaagttgcaa aagaaattat
    20101 tgctataaat aaaccattat ttggaatttg tctaggacat caagttatcg gtttggccaa
    20161 tggtgtttca acatataaaa tgtttaacgg gcacagaggt atcaatcatc cggttaaaaa
    20221 tctaatgacc ggaaaaggag aaattacttc tcaaaaccac ggatttgcag tggttagaga
    20281 agaacttgaa aaaaatcctg atttagaaat tactcacgaa catttaaatg atggaactgt
    20341 tgccggaatg cgaatgaaat ctaaaaattg tttctcggta cagtaccatc cggaagcaag
    20401 tccgggaccg catgattcat cctatttgtt tgatgatttc attgatatta ttcaaaaatc
    20461 aaaattacaa gaggtataaa atatcaaata cctgacattt ttttaatgtc aggtcttttt
    20521 ttacactaaa acgattgcgt ttataacttt cttaaattat ggataaatcc aaatttagaa
    20581 tgcttaaatt tgtgtatcat tcaaaaaaaa caataaaaaa atagtaaata aacagtatca
    20641 tgagtattat tattaaagtg catgcaagac aaattttgga ttcaagagga aatccaacta
    20701 ttgaagttga tgttattaca gaaaacggaa ttttaggaag agctgcagtt ccatctggag
    20761 cttctacggg agaacatgaa gcagtagaac ttcgtgatgg gggaaaatca tatatgggaa
    20821 aaggtgtttt aaaagctgtg gataatgtta atacaaccat tgctgatgaa attgttggaa
    20881 tgtctgtttt tgagcaaaat caaattgaca aaactatgat tgaattggat ggtacaccaa
    20941 ataaagctaa cttaggtgca aatgcaattc ttggcgtttc attagctgtt gcaaaagctg
    21001 ctgccaatga attaggcatg ccattgtatc gttatgttgg tggggtttct gctaatacac
    21061 ttcctgttcc aatgatgaat atcatcaatg gcggatcaca ttctgacgct ccaattgcat
    21121 ttcaggaatt tatgattatg cccgtaaaag caaccagttt tacacatgct atgcaaatgg
    21181 gtactgaaat atttcataac ttaaaaaaag tattacacaa ccgtcattta tcaacagcag
    21241 taggtgatga aggtggtttc gcaccaaatt tagcaggagg aacggaagat gcaattgaat
    21301 caatcaaaaa agcagtagaa aatgctggat atgcttttgg tgaagatgtg atgattgctc
    21361 ttgattgtgc cgcttctgaa ttttatgtgg atggaaaata tgattataaa aaatttgaag
    21421 gagaaacagg aaaaatacga acttcagctg aacaagctga atatttagca gatttagctt
    21481 caaaatatcc aattatttcc atcgaagatg gaatggaaga aaatgattgg gaaggttgga
    21541 aatatttaac ggatttaatt ggcaataaaa ttcaattagt aggtgatgat ttgtttgtta
    21601 cgaatgttga gcgtttaaaa accggtattg acaaaggtat tgctaattcc atcttggtta
    21661 aggttaacca aattggaaca ctaacagaaa caattgctgc tgtaaatatg gctcataatg
    21721 ccggttacac ttcagtaatg tcacaccgtt ctggagaaac tgaagataat accatcgctg
    21781 atttagccgt agcgttaaat tgtggtcaaa ttaaaaccgg ttccgcttct cgttcagatc
    21841 gtatggcgaa atataaccaa ttactacgta ttgaagagga attaggaaat acagcttatt
    21901 ttcctgggaa gaaagctttc aaacagaatt aaaaattagt ttataatgtc tatttaaccc
    21961 tttcggaatt tatttctgaa agggtttttg ttttattgaa aattatagaa cgattttttt
    22021 tatattagcc tactcaacaa caaattagaa cacaatgaaa aaaaattact ttgcaatatt
    22081 aagttttatc gtttttgtat ttaattcaaa tgctcaagtt actattccta tttatgataa
    22141 tgttttattt tatgacggat atgcttcttt ggttaattac ccaacagaac cggatgttat
    22201 aaggttaaga aatgatttat ttacgaaaaa gttgactcct gaagttttag ctcaaatagg
    22261 aacttcgctt actttaaata tttcaatttc tgcggcatgt gacaattatg atagaattgg
    22321 aaatattaat ttagcgttag ttccaaaagg agcaacttct tataatccgg atacaacaaa
    22381 tagattagaa ttaggaaggt atataactcc ttttatgaat aaaaatgttc cgccaaattt
    22441 agtgccatat acttttagtg tagataatat tacaaaaatt ctaaaagata cttatctaaa
    22501 cacaatttat gatttttgga ttgagtttga attatttggc gtgccatatg ctgctcagac
    22561 tcaaatttct ggatgttcag gaagaaatga tgtgttttat ggtactttaa attttataac
    22621 aaatacggaa acttcaagtc ctaacaatac ttacattcat actttaaatt ttaaaaaaat
    22681 attaaatgat taccaagctg gggcttctga tggtattttt caaacagtaa gaactatcta
    22741 ttttgatttg ccatcagcca ttaataatgc aaaattttat ttaattacgt caagtcatgg
    22801 tgcaaatgca ggtggagaag aatacaatag aagatggcat tatatttcat taaatagtaa
    22861 tcaaattcta tcgtatttac cgggagaacc cacttgtgaa ccttacagag ttttcaatac
    22921 acaaggaaat ggaatttatg gtcctacacc gagaactccg gaagaatggc aatctttcag
    22981 caattggtgt ccgggtgcag ttattcctat aagagaaatg gatcttggaa atttagcagc
    23041 tggaaatcat atgttcagga ttgctgttcc aactgcggtt ttcgttggaa atgaagggta
    23101 cattcccgtt tcgttatatt tgcaaggcga aaatacagct ttaaatgttg atgattttga
    23161 ggcaatcatt gttgaccatt atcctaaccc tacaaaaaat aaagttttta taagttgccg
    23221 gaaaattatc gatgctatcg aaatcgttga tttacaagga aaaataattg aatccaaagt
    23281 agtagaaaat actgaaactg aagtggattt cacacaattt tcaaatggtg tttattttat
    23341 aaaaatgaag ttaggagagg taaccaaaac acataaaatt attaaagaat aatggaatta
    23401 actaaaaacc ctttcaaata ctgagagggt ttttgttttt actcaaatac tcactcaaaa
    23461 cctgcaatta ct
//
LOCUS       DAOTCW010000021        23389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1132_length_23389_cov_39.091026, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000021 DAOTCW010000000
VERSION     DAOTCW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 23389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23389
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1132_length_23389_cov_39.091026"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /gene="sucD"
                     /locus_tag="P5335_03095"
     CDS             complement(<1..830)
                     /gene="sucD"
                     /locus_tag="P5335_03095"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRZ73901.1"
                     /translation="MSVLVNKDSKIIVQGFTGSEGTFHASQMIEYGTNVVGGVTPGKG
                     GTTHLERPVFNTVKDAVLQAGADTTIIFVPPAFAADAIMEAADAGIKVIITITEGIPV
                     ADMIKAYAYIKDKGCRLIGPNCPGVITPEEAKVGIMPGFVFKKGNVGIVSKSGTLTYE
                     AADQVVKQGLGITTAIGIGGDPIIGTTTKEAIELLMNDPETKCIVMIGEIGGQLEADA
                     AKWIKADGNRKPVVGFIAGETAPKGRTMGHAGAIVGGADDTAEAKKRIMRECGIHVVD
                     S"
     gene            complement(951..1313)
                     /locus_tag="P5335_03100"
     CDS             complement(951..1313)
                     /locus_tag="P5335_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRZ73902.1"
                     /translation="MNAKELVLDFYNSDALYNKNTMDVFLHPDFSLEWNSSKGLIRKD
                     REGLLQLAEELKVGYETFVVQIHQILAEDNLVSVHYTHFGTTIENPRQINRLAYFFVI
                     WEIQDGKLFRGYQMSQLP"
     gene            complement(1315..2244)
                     /locus_tag="P5335_03105"
     CDS             complement(1315..2244)
                     /locus_tag="P5335_03105"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRZ73903.1"
                     /translation="MKFPKTYSLKEIAQIIGSEFVGDENFSVQGMNEIHVVEHGDIVF
                     VDHPKYYDKALHSAATIVLINKEVECPEGKALLISDDPFRDFNKLTNHFRPFTFSNVS
                     IAPTAQIGEGTKIQPNCFVGNHVKIGKNCLLHANVSIYDHCVLGDNVIIQAGTIIGAD
                     AFYYKKRPEGFDQLISGGRVIIEDNVGIGALCTIDRGVTGDTTIGAGTKIDNQVHVGH
                     DTVIGKKCLIAAQTGIAGCVVIEDEVTMWGQVGTTSGITIGTKAVVMGQTGVTKSIEG
                     GKTYFGTPIEESREKLKQLAYIKRIPEILEKLK"
     gene            complement(2275..>2814)
                     /gene="efp"
                     /locus_tag="P5335_03110"
     CDS             complement(2275..>2814)
                     /gene="efp"
                     /locus_tag="P5335_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRZ73904.1"
                     /translation="GLCIKHNHDIYKIIEFLHVKPGKGPAFVRTKLKSLTNGKVLDNT
                     FSAGHKIDVVRVETHTYQFLYSEGTEFYFMNTESFEQITLNRDILDAPDLLKEGTLVM
                     VQINTETDLPLSVDMPASIVLEVTYTEPGVKGNTATNATKPAKVETGASVNVPLFINE
                     GDKIKIDTASGNYMERVKE"
     assembly_gap    2815..2914
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2915..3549)
                     /gene="lpxA"
                     /locus_tag="P5335_03115"
     CDS             complement(<2915..3549)
                     /gene="lpxA"
                     /locus_tag="P5335_03115"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRZ73905.1"
                     /translation="MNQPLAYVHPGAKIARNVVIDPFTTIHNNVVIGEGTWIGSNVTI
                     MEGARIGKNCNIFPGAVISAVPQDLKFGGEDSLAIIGDNSTIRECVTINRGTIASGQT
                     TIGKNCLIMATAHVAHDCHIGDNAIIVNGVALAGHVTVGNFAIIGGLAAVHQFINIGD
                     HAMISGGSLVRKDVPPYTKAAKEPLSYVGINSVGLRRRGFTTEKIREIQEI"
     gene            complement(3560..4957)
                     /locus_tag="P5335_03120"
     CDS             complement(3560..4957)
                     /locus_tag="P5335_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HRZ73906.1"
                     /translation="MSQSVKQKTIKSEVTLKGVGLHTGQEVIMTFKPAPVNNGFTFVR
                     VDLEGHPIIEADANHVVNTQRGTNLEKNAVKIQTSEHVLAACVGCDIDNLIIELNASE
                     PPIMDGSSKYFVEALEKAEVVEQDADRKVYVVKEVISYTDETTGSEILVMPCDSYQVT
                     TMVDFGTKVLGTQNATLKSISDFKKEIANARTFSFLHELESLLNNGLIKGGDLNNAIV
                     YVDKEISPETMDNLRVAFGKDKISVKPNGILDNLTLHYPNEAARHKLLDVVGDLALVG
                     TRIQGKVIANKPGHFVNTQFAKKLAKIIKIEQRNQVPTYDLHQEPLMDIHKIMAMLPH
                     RPPFLLVDKIFELSDHHVVGLKNVTMNEPFFVGHFPDAPVMPGVLIVEAMAQTGGILV
                     LSTVPDPENYLTYFMKIDNVKFKHKVLPGDTLIFKCDLISPIRRGICHMQANAYANGK
                     LVAEAELMAQIAKKN"
     gene            complement(4960..>5583)
                     /gene="lpxD"
                     /locus_tag="P5335_03125"
     CDS             complement(4960..>5583)
                     /gene="lpxD"
                     /locus_tag="P5335_03125"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRZ73907.1"
                     /translation="NVKIYPNSFIGDNVSIGDNCVFFAGARIYSETEIGNNCTIHSGT
                     IVGSDGFGFAPQEDGTYEKVPQIGNVIIEDNVEIGSCSTIDRATLGSTIIRKGVKLDN
                     QIQVAHNVEIGENTVIAAQTGIAGSTKIGKNCMIGGQVGIVGHLIIGDGVRIQAQSGI
                     GKSLKDGETVQGSPSFNYGDFSKSYVHFKNLPKIVSEIEELKKTIIK"
     assembly_gap    5584..5683
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5684..5817)
                     /locus_tag="P5335_03130"
     CDS             complement(<5684..5817)
                     /locus_tag="P5335_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRZ73908.1"
                     /translation="MKFTATQIASILEGEVVGNPNVEVYKLSKIEEGSAGSLTFLANP
                     "
     gene            complement(5852..7087)
                     /locus_tag="P5335_03135"
     CDS             complement(5852..7087)
                     /locus_tag="P5335_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRZ73909.1"
                     /translation="MSQTNKLKIFNDPIYGFITIPNELVFDLIQHPYFQRLRRITQMG
                     LSYLVYPGANHTRFHHALGAMHLMQKAVEVLRFKEISISKEEETALYVAILLHDIGHG
                     PFSHAMEQSIVENVHHEEISLLFMNQLNRAFDGKLSLAIQIFKGEYHRKFMLQLISSQ
                     LDMDRMDYLKRDSFYSGVAEGNINSDRLIQMLHVVDDVLVMEEKAIYSIEKFLMARRL
                     MYWQAYLHKTSLVAELILTRTLKRAKELTQKGTVLACSEPLQFFLQNKVDFADFDAII
                     LDKFAQLDDFDIVSALKAWQYHPDFILSSLSKMIINRDLLKIKVSNEKFDKEEIKLYK
                     HKLLEYYPISLAETDYFVFKGKIKTLGYNKVIEPIRILKKDKTVEDVVESSEQLNSRA
                     FSKPVTKYYSCFPKVLIEK"
     gene            7217..>8220
                     /locus_tag="P5335_03140"
     CDS             7217..>8220
                     /locus_tag="P5335_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HRZ73910.1"
                     /translation="MDKIKILWVDDEIDLLKPHILFLETKNYAVTTCNNGRDAVDIFE
                     ENQFDIVFLDENMPGMSGLETLSEMKEKKSAVPIIMITKSEEESIMEEAIGSKIADYL
                     IKPVNPNQILLALKKNLDHSRLISEKTTLDYQKEFRKIAMDLAVVNSFEDWTELYKKL
                     IFWEIELENIEDQGLISILESQKTEANSQFGKFIEKNYEKWFEDKADKPILSHKLFRE
                     GVVPELVKKDKPILFVVIDNLRYDQWKAFESVVNNHYKLEKEMAYFAMLPTATQYARN
                     AIFSGLTPLEMEKQFPQYWLNDVDDGGKNLFEGEFLAAQLKRLGLNIKSEYYKITNSR
                     "
     assembly_gap    8221..8320
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8321..8538
                     /locus_tag="P5335_03145"
     CDS             <8321..8538
                     /locus_tag="P5335_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HRZ73911.1"
                     /translation="EEKDVYAVKDPKKIGLPTINMSSSFIFAKNDLFLAYVNNYNHYV
                     SYYRNSYQHGGISLEEMIVPFLVFNPK"
     gene            8629..9033
                     /gene="tsaE"
                     /locus_tag="P5335_03150"
     CDS             8629..9033
                     /gene="tsaE"
                     /locus_tag="P5335_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRZ73912.1"
                     /translation="MEFIYNLNEIDAIAQQVIAQNPQKVILFNGLMGVGKTTFIKALS
                     KRLGVEKPTSSPTFSLVNEYQAKEGLVYHFDVYRLKNEMEALDFGIEDYLYSGNWCFI
                     EWAEKIPNLIPEKHNVITIEILDNGNRKLILD"
     gene            9197..10390
                     /locus_tag="P5335_03155"
     CDS             9197..10390
                     /locus_tag="P5335_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRZ73913.1"
                     /translation="MPLTPFSKQQLLPQEEKLEIARHKSELFIGIPKETSYQERRICL
                     TPDAVSSLVSHGHRVLLEAGAGESASYSDKEYSDAGAEITQDPKKVFSCPMILKVEPL
                     TSAEIEMINMNTIVISAIQLKTRKKEYFEKLAKKKITAFAFEFIKDDDGSYPAVKSLS
                     EIAGTASILIAAELMINNQFGKGLLFGNITGVAPTDVVIIGAGTVGEFAAKTAIGLGA
                     NVKVFDNSITKLRRLQNNLKQPIFTSTIQPKSLLKALRRCDVAIGAIRGQNRTPVVVT
                     ETMVEHMKKGAVIVDVSIDTGGCFETSEITTHEKPTFIKNNVVHYCVPNIPSRYSKTA
                     SLSISNILTPYLIQIAEDGGVESAIRCNRGLKNGLYFYHGILTNKSIGDWFDLPNSDI
                     NLIVF"
     gene            10431..10814
                     /locus_tag="P5335_03160"
     CDS             10431..10814
                     /locus_tag="P5335_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HRZ73914.1"
                     /translation="MKFYQRFAFFLFGLLIGIIFLIYFLGAKAKSRGVTFCYLPNCRV
                     LKDIRSKPFFYSEKSSKVLAESWIDTVDIKNTLTYGEVDFSKSNIKEGAAKLYIIEGK
                     TINQVPVTLEVLNYESKVVLKEIKK"
     gene            10821..>11941
                     /gene="pepT"
                     /locus_tag="P5335_03165"
     CDS             10821..>11941
                     /gene="pepT"
                     /locus_tag="P5335_03165"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF003976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRZ73915.1"
                     /translation="MQHIIDRFISYVTIDTESDPNSETTPSSKKQLVLAKNLVKELKT
                     IGMEDVTIDKNGYVMATLPSNVEHEVPTIGFVSHYDTTPDFIGANVKPQIIENYDGKD
                     IVLNAEQNIILSPSYFKDLLQYKGQTLITTDGTTLLGADDKAGITEIVTAMEYLIKNP
                     EIKHGKIRVGFTPDEEIGRGAHHFDVEKFGAEWAYTMDGSQIGELEYENFNAAGAKIT
                     FKGKSVHPGYAKGKMINSMLLASKFISKLPKKEVPEETKGYEGFFHVTGITGSIEEST
                     VSLIVRDHDKKIFKKRKDFLHELTAKFNKKYQKQFGEDIVIIEIKDQYFNMKEKVKPV
                     FHIVEIAEKAMKKLDIKPIIKPIRGGTDGCQLSYKGLPCP"
     assembly_gap    11942..12041
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12042..12163)
                     /locus_tag="P5335_03170"
     CDS             complement(<12042..12163)
                     /locus_tag="P5335_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRZ73916.1"
                     /translation="MENLQSLLPFLLMFVVIYFFMIRPQQKRAKQEKEFESSLKV"
     gene            complement(12165..12740)
                     /locus_tag="P5335_03175"
     CDS             complement(12165..12740)
                     /locus_tag="P5335_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013188323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRZ73917.1"
                     /translation="MIKKSMYVMAVLAFALTTSCKKEDALSKVDPTSSDVPMNYEAKQ
                     VDANGNPMPVQPVTENGQTPEQAASAPIDGKYPVMTFGKKEHNFGVINEGDKVETYFT
                     FTNTGDADLIISNASGSCGCTVPDYPKEPIKPGKSSKMKVTFDSNGKPGKQQKSVTIV
                     ANTLSTREVLTINAEVTPKVKPVETQTTVNQ"
     gene            complement(12759..13670)
                     /gene="nusB"
                     /locus_tag="P5335_03180"
     CDS             complement(12759..13670)
                     /gene="nusB"
                     /locus_tag="P5335_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRZ73918.1"
                     /translation="MQIIYAMHQSGSEDLEKEEKFLFSSLESIQDLYLIVVSTLIEIK
                     KKEEVFLEKASKKHLATAEDLKPNRKFIANKVLQFLEDNNSISIALKNRKINNWSQND
                     DLILIILDEIKRSAIYQNYLADTSSSFEADKKFVADIFTELIASNEKLYDYLEDFRIT
                     WVDDIPLVNTSLLKQLKHIEEHNEKSFSVLPLYKDAEDKDFARDLFRKTVLNENDLAK
                     EYIDRTPNWDPERISEVDTIMLKMAICEFLKFPSIPIKVTINEYLEIAKEYATPKSSI
                     FINGILDNLVKDFQKTDKLKKTGRGLM"
     gene            complement(13780..14883)
                     /locus_tag="P5335_03185"
     CDS             complement(13780..14883)
                     /locus_tag="P5335_03185"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRZ73919.1"
                     /translation="MIAEVTTPKELQKMDPVFGQVSFDNHEQIVFCNDKDTGLKAIIG
                     IHNTVLGPALGGTRMWNYTNEWEALNDVLRLSRGMTYKSAITGLNLGGGKAVIIGDAK
                     TQKTPELMRKFGEFVHSLSGKYITAEDVGMQTEDMDTVRDVTPYVTGISESRGGSGNP
                     SPVTAFGVYMGMKAAAKYKYGSDSLAGKKVLVQGIGHVGETLVDYLTKEGAIVTIADI
                     NINRLNEVGAQYGAKVFTGSDLYSADVDIYAPCALGATINDDTVYKLNASIVAGAANN
                     QLANENVHGKILQERGILYAPDFLINAGGIINVYAEIEHYDKAESMRRTENIYHTTLE
                     IFDYAVKNNITTHQAALTIAQNRIDQRKKESGK"
     gene            15086..>15930
                     /locus_tag="P5335_03190"
     CDS             15086..>15930
                     /locus_tag="P5335_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRZ73920.1"
                     /translation="MKELHYLNKYFIKYKYRFLLGILITIVAQIFSLFTPKLISKSLN
                     IIEEYIKNPIKDSSLIKQELLLSLGLIIGATIIAGFLTFLMRQTLIVMSRYIEFDLKN
                     EVFQHYERLSQSFYKQNRTGDLMNRISEDVGKVRMYVGPAVMYTINTFIRFAIVILYM
                     YNVSPKLTLYTLLPLPLLSYCIFKLSSEINKRSTIYQENLSNLSSFSQETFSGIRVIK
                     AYALEGQNKKAFNNLSQESREKNMRLAVVNALFGPLMILLIGLSNLVVIYFGGMMYIE
                     GTIPN"
     assembly_gap    15931..16030
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16031..16339
                     /locus_tag="P5335_03195"
     CDS             <16031..16339
                     /locus_tag="P5335_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HRZ73921.1"
                     /translation="QKQRVSIARAIIKDPEILLFDDCLSAVDTETEEQILNNLFEICK
                     NKTTVIVSHRVSSAKNADKIIILEEGEILQQGTHSQLVNQEGYYKELYLKQLSEKELS
                     "
     gene            16410..16787
                     /locus_tag="P5335_03200"
     CDS             16410..16787
                     /locus_tag="P5335_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PUR family DNA/RNA-binding protein"
                     /protein_id="HRZ73922.1"
                     /translation="MRENEMLEKDEIFSKVLRAGRRTYFFDVRATKADDYYITITESK
                     KFTEEDGSFHFKKHKIYLYKEDFVAFKEILDDMTSYVLDHKGEEVISERHQKDFKKEY
                     AVDADETEDSPKASFTDIDFDDI"
     gene            complement(16823..17155)
                     /locus_tag="P5335_03205"
     CDS             complement(16823..17155)
                     /locus_tag="P5335_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HRZ73923.1"
                     /translation="MLTWEEFEKVEMRIGTILEVNDFPEARKPAYQLIIDFGDEIGIR
                     KTSAQITKRYSKDVLIGKQIVAVINFPKKQIGKFNSECLVLGSVGEENDIVLLTSDVK
                     VANGLRIG"
     gene            17248..17805
                     /locus_tag="P5335_03210"
     CDS             17248..17805
                     /locus_tag="P5335_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRZ73924.1"
                     /translation="MARTASKMSSLGTLAPNFLLKDTNSGNFFTFSDCKGEKGTLVFF
                     ICNHCPFVHHVMEEIIMVANDYRVQGIGIVAISSNDVVQYPQDSPELMTEFAFENKFE
                     FPYLYDETQEVAKAFDAACTPDFFLFDNQDKLVYRGQLDDSRPGNGIPLSGSDLRGAI
                     DSLLYNRIINPNQKPSIGCNIKWKK"
     gene            17806..19224
                     /locus_tag="P5335_03215"
     CDS             17806..19224
                     /locus_tag="P5335_03215"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HRZ73925.1"
                     /translation="MEIVHISAECYPVAKVGGLADVVGALPKYLTKLGHLTKVVVPGY
                     DTKFSRENEFVSVHQGLLKLGWFQFSYTVFKEKTNKLGFELYLIAIPELFDRPNVYSY
                     EDDTERFTAFQIAFLNWMTETNQIPDIIHCHDHHTGLIPFMMSHAPIYQHLNRVPTLL
                     TIHNGQYQGWFGFDKLHYLPKVSSSRLGFLEWNGMINPLASAIKCAWKVTTVSPSYLD
                     ELSEKANGLENLLRFERPKCLGILNGIDSEVWNPDTDEMLENKYSIKNVKAGKAKNKK
                     FLCEQFNLDPSKPLFTFIGRLVGEKGADLLPEICSIALNRNPNEINILILGSGDPNVE
                     HGLTQLTHFYNGNYNTYIGYNENLAHRIYAGADYLLMPSRVEPCGLNQLYALRYGTIP
                     IVRRTGGLKDTVLDIGDGGFGICHDHTSVYDVNHSITRAIDLFNDTKQFDSIRKIIMQ
                     IDHSWDKVAQEYVEVYQSLKNN"
     gene            19226..20500
                     /locus_tag="P5335_03220"
     CDS             19226..20500
                     /locus_tag="P5335_03220"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRZ73926.1"
                     /translation="MKKSVLAIILGGGQGSRLYPLTESRSKPAVPIAGKYRLVDIPIS
                     NCINSDIKRMFVLTQFNSASLNQHIKNTYHFSYFSTAFVDILAAEQTPDNPTWFQGTA
                     DAVRQCMQHFLNHEFDYALILSGDQLYQMDFNLMIEAHQKSGANITIATLPVSAKEAT
                     DFGILKTDENSLITSFIEKPATDLLPQWTSEVSDEMKKEGKHYLASMGIYIFNRDLLI
                     ELMSNPTTKDFGKEIIPQAIGNQKILSYQYEGYWTDIGNIDSFFEANLGLTDSIPKFN
                     LFDNSSKIYTRARVLPPSKILGKTYIEKSMVAEGCILNSAKIEHSVIGIRARVDEDTT
                     IQNSYVMGSDFYQNLDEISHNNENNIINIGIGKRCFINNTIVDKNCRIGNDVYLNGGH
                     HLEDANTPFYTIKEGIIVVKKGATLPDGFRLE"
     gene            complement(20537..21088)
                     /locus_tag="P5335_03225"
     CDS             complement(20537..21088)
                     /locus_tag="P5335_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73927.1"
                     /translation="MRFLFLFFILFYYTAQSQVINVQHCGYDFTSYLVINVHEQGKTE
                     NIKNLKITIVDSSGSDVINKNNRWSWVNNNKPLLFTPNYKLDRNNNKVENSNPEGKWF
                     FPFAKDYYLLSVVNTFTADIFSVKIEDIDMEENGGYFETQIIPLSPYNMYILCSSENE
                     RQAQQFGPRTNRPVDVILKKKSY"
     gene            complement(21248..22180)
                     /locus_tag="P5335_03230"
     CDS             complement(21248..22180)
                     /locus_tag="P5335_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRZ73928.1"
                     /translation="MENGIYAKFNTTKGPILVKLTHDKTPGTVGNFVGLAEGQLENEA
                     KPMGKPYYDGLKFHRVIADFMIQGGCPQGLGTGGPGYQFDDEFHPDLRHDGPGVLSMA
                     NSGPGSNGSQFFITHVATPWLDKKHTVFGNVVEGQDVVDAIAQGDVLETLEIIRVGDE
                     AQKWNAIETFRTFEGSREKRIAEEKAKAEEALEKLAAGFEKTESGLRYKIIQKGNGKQ
                     AEKGKTVSVHYSGSLENGVVFDSSYKRKQPIDFPLGKGHVIEGWDEGIQLLQVGDKAR
                     FVIPSFLGYGSRGAGGVIPPDATLIFDVELMDVK"
     gene            22272..22721
                     /locus_tag="P5335_03235"
     CDS             22272..22721
                     /locus_tag="P5335_03235"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HRZ73929.1"
                     /translation="MESLFDKSDNAAIIQRIHSLNSDSKPLWGKMNVAQMLSHCQAPL
                     DVATGDLQLKSNFILQFIGKIYKKKILAAPGFKKNSPTAPDFLRNNPVDFEQAKLELI
                     EKVLKFSTLGPKVIANHKHPFFGEMTNEEWDLLQWKHLNHHLNQFSV"
     gene            22723..>23389
                     /locus_tag="P5335_03240"
     CDS             22723..>23389
                     /locus_tag="P5335_03240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRZ73930.1"
                     /translation="MENLPDYININKETWNKKTAVHIASDFYNNEAFLNGKSSLNHFE
                     IDLLGDVSQKKILHLQCHFGQDTLSLARLGAKVTGIDFSDQAIEKAKEYSKQLDLDAT
                     FICCDVYDTPNYLDEKFDIVFTSYGTIGWLPDMDKWAAVVSHFLKPEGKFVFVDFHPV
                     VWMFDDDFKEVFYNYFKSEPIIEEETGTYADKKASLIQKTISWNHSISEVVNALIENG
                     LTIH"
BASE COUNT         7554 a         3976 c         3581 g         7778 t
ORIGIN      
        1 gaatcaacca catggatacc acattctctc atgattcgtt ttttagcttc tgctgtgtca
       61 tcagcaccac caacaatagc accggcgtgt cccatggttc ttccttttgg tgctgtttct
      121 ccggcaatga aaccaacaac aggtttacga tttccgtcag cttttatcca tttagcagca
      181 tcagcttcta actgacctcc tatttcacca atcatgacaa tacattttgt ttccggatca
      241 ttcatcaata attcaatagc ttctttagta gttgttccaa taattggatc tccaccaatt
      301 ccgatagcag tagtaatgcc taaaccttgt ttaacgactt gatcagctgc ttcataggtt
      361 aacgttcctg attttgatac gattcccaca tttccttttt tgaatacaaa acccggcata
      421 ataccaactt ttgcttcttc cggagtgatt acgcccgggc agtttggccc gattaatcga
      481 catcctttat ctttaatata agcatacgct ttaatcatat cagctacagg aataccttct
      541 gtaatggtaa tgattacttt tattcctgca tctgcggctt ccataattgc atcagcagca
      601 aaggctggtg gtacaaaaat aatggtcgta tctgctcccg cttgtagaac tgcatctttt
      661 acagtgttga aaaccggacg ttctaaatgg gttgtgcctc cttttccggg tgtaacgcca
      721 ccaacaacat ttgttccgta ttctatcatt tgtgaagcat gaaaagttcc ttcgcttccg
      781 gtaaatcctt gtactattat ttttgaatct ttattgacta aaacgctcat gattgtgttt
      841 gtttgttttt aatttttgta ttacaaaagt aattctttag aaaaaatata gctcactttt
      901 tttatcgatt tttaaataaa gttgctaaat taatatataa tttaaggtaa ttatggtagt
      961 tgactcattt gataaccacg aaataatttg ccatcttgaa tttcccatat cacaaagaaa
     1021 tacgctaaac ggttaatttg cctcggattt tcaatagtag tcccaaaatg ggtataatga
     1081 accgacacta aattatcctc agccaaaatt tggtgaattt gaacaacaaa tgtttcatat
     1141 cccactttca attcttctgc caattgaagt aagccttccc tgtcttttct aatcaagcct
     1201 ttactactat tccactctaa cgaaaaatca ggatgtaaaa aaacatccat tgtatttttg
     1261 ttatacaaag catcggaatt ataaaaatct aaaaccagct cttttgcatt cattttattt
     1321 taatttttct aaaatttcag gaattcgttt gatatatgcc aattgtttta atttttctcg
     1381 tgactcttca attggagttc caaagtaagt tttaccacct tcaatagatt ttgtaactcc
     1441 ggtttgtccc atcacaaccg ctttagttcc aatagttatt ccacttgttg ttcctacttg
     1501 cccccacatt gttacttcat cttcaataac tacgcaaccg gcaattccgg tttgagcagc
     1561 gattaggcat ttttttccga ttactgtatc atgacccaca tgaacttggt tgtcaatttt
     1621 agttcctgct ccaatcgttg tatctcctgt tactcctcta tcaatagtac acaaagctcc
     1681 aattcctaca ttatcttcaa taattactcg gccgccggat attaattgat caaaaccctc
     1741 aggtcttttt ttataataaa aagcatcggc acctataatt gttccggcct gaataatgac
     1801 attatcaccc agtacacaat ggtcatagat tgatacatta gcatgaagta gacaattttt
     1861 tccaattttt acatgatttc caacaaaaca attgggttga attttggttc cttcaccaat
     1921 ttgagctgtt ggagcaatcg aaacatttga aaaagtaaac ggtctaaaat gatttgtcaa
     1981 tttattaaaa tcacgaaacg gatcatcaga aatcaacaat gcttttcctt ccggacattc
     2041 tacttcttta ttaattaaaa caatagtcgc ggccgaatgt agagctttat cataatattt
     2101 ggggtgatct acaaaaacaa tatccccgtg ctcaaccaca tgaatttcat tcataccctg
     2161 aaccgaaaaa ttttcatctc ccacaaattc actaccgata atttgggcta tttctttaag
     2221 cgaatatgtt tttggaaatt tcataaactt tgaaattgat attgttgtta aaaactattc
     2281 tttcacacgc tccatgtaat ttccggaagc tgtatcaatt tttattttat ctccctcatt
     2341 gataaacaaa ggaacgttta ctgatgcacc ggtttctact ttggcaggtt tagtagcatt
     2401 tgttgccgta tttcctttaa cacccggttc ggtataagta acttctaaaa ctatagaagc
     2461 aggcatatct actgataatg gtaaatcggt ttcagtatta atttgaacca tcacaagagt
     2521 cccttctttt aataaatcag gagcatctaa aatatctcta tttaaggtaa tttgctcaaa
     2581 actttccgta ttcatgaaat aaaattcagt tccttctgaa tataaaaatt ggtacgtgtg
     2641 tgtttcaacc cgaactacat caattttgtg tcctgcagag aaagtattat ctaatacttt
     2701 tccgtttgtt aaacttttta atttagttct cacaaaagcg ggacctttac ccggttttac
     2761 atgaagaaat tcaataattt tgtagatgtc gtggttgtgt ttaatacata atccnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntatatt tcttgaattt ctctgatttt
     2941 ttctgtggta aatcctcttc ttcttaatcc aactgaatta attccaacat aagataatgg
     3001 ttcttttgca gcttttgtat aaggtggaac gtcttttctg accaatgaac ctcctgaaat
     3061 catggcgtga tcaccaatgt tgataaattg atgaacagct gctaaacctc caataatagc
     3121 aaaattaccc acagtcacgt gacctgctaa ggcaacacca ttcacaataa ttgcattatc
     3181 gccaatgtgg caatcatgtg ctacgtgtgc ggttgccata attaaacaat ttttaccaat
     3241 tgttgtttga ccggaagcaa tggtgcctcg attaattgtt acacattcac gaatagtaga
     3301 attatcacca ataattgcca gtgaatcttc tccaccaaat tttaaatctt gtggaactgc
     3361 tgaaattaca gctcccggaa aaatattaca atttttacca attcgggcac cttccataat
     3421 agtaacgttg gaaccaatcc aagttccttc tccaataacg acattattat gaatggttgt
     3481 aaacgggtcg ataactacat ttcgtgctat ttttgctcca gggtgaacgt atgctaatgg
     3541 ttgattcata cttgatgtat tagttctttt ttgctatttg agccatcagc tcggcttctg
     3601 caaccaattt tccgtttgca tacgcattgg cttgcatatg acaaattcct cttcgtattg
     3661 gagaaattaa atcacattta aaaattaatg tatcgcccgg taacactttg tgtttgaact
     3721 ttacgttatc aattttcata aaataagtca ggtaattttc aggatcagga acagtactta
     3781 gcactaaaat tccacctgtt tgagccattg cttccacaat taaaacaccc ggcattactg
     3841 gtgcatctgg aaagtgacca acgaaaaacg gttcattcat ggttacattt tttaaaccaa
     3901 caacatgatg gtccgataac tcaaaaattt tgtcaaccaa taaaaatggc ggacggtgag
     3961 gcaacatagc cataattttg tgaatatcca tcagtggttc ttgatgcaaa tcgtatgttg
     4021 gaacctgatt acgttgctct atttttatga ttttagcaag ttttttggca aactgcgtat
     4081 ttacaaaatg accgggttta ttggcaataa ctttcccttg aatgcgggta ccaactaatg
     4141 ccaaatcacc aacaacatct aataatttgt gccgagcggc ttcattagga taatgtaaag
     4201 tcaaattatc taaaataccg tttggtttta ctgaaatttt atctttacca aatgcaactc
     4261 tcaagttatc cattgtttcg ggagagattt ctttatcaac atacacaatg gcattattta
     4321 aatctcctcc tttaatcaaa ccattgttta aaagagattc taactcatgt aaaaaactaa
     4381 acgttctggc attagcaatt tcctttttaa aatctgatat agattttaaa gtagcatttt
     4441 gagttcctaa tactttagtt ccaaaatcaa ccatcgtggt aacttgatag gaatcacaag
     4501 gcatgactag aatttcacta cctgtagttt catctgtata ggaaataact tctttaacta
     4561 catatacttt tcgatcagca tcttgttcaa ctacttctgc tttttctaaa gcttccacaa
     4621 aatatttaga tgaaccatcc ataataggtg gctcagaggc atttaattct ataattaggt
     4681 tgtcaatatc acatcctaca caagcggcca aaacatgttc tgaagtttga atttttactg
     4741 cattcttctc taaattggtt cctctttgcg tatttaccac atgatttgca tcagcttcaa
     4801 tgatgggatg accttctaaa tctacacgaa caaaggtaaa cccattattt acgggagccg
     4861 gcttaaaagt catgataact tcctgaccgg tgtgtaatcc aactcctttt aaagttacct
     4921 cgctttttat ggttttttgt ttaaccgatt gtgacatatt tatttgatta tcgttttttt
     4981 aagttcttct atttcactga ctatttttgg caaattttta aaatgcacgt aggatttact
     5041 aaaatcacca taattaaaag agggcgagcc ctgaaccgtt tcaccatctt ttaaactttt
     5101 tccaataccg gattgggctt gaattcgaac accatcacca ataataagat ggcccacaat
     5161 tccaacttgt ccgccaatca tacaattttt tccaattttt gtagaaccgg caattcctgt
     5221 ttgagccgca ataacggtat tttccccaat ttctacatta tgagcaactt gaatttggtt
     5281 atccaatttt actccttttc taattattgt tgaacctaaa gtagctctgt ctatagttga
     5341 acaagagcct atttcaacat tatcttcaat aatcacattt ccaatttgtg gcactttttc
     5401 ataggttccg tcttcttgag gagcaaaacc aaaaccatca gatcctacaa ttgttcctga
     5461 atgaatcgtg caattattac caatttcagt ttcagaatat attctggcac cggcaaaaaa
     5521 cacacaatta tcacctatag aaacgttatc accaataaaa ctattcgggt aaatttttac
     5581 attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttggat tggctaaaaa
     5701 agtaagagaa ccggcagatc cttcttcaat tttggataat ttatagactt caacattggg
     5761 atttccaacg acttcgcctt ctaaaatgga tgctatttga gttgcagtaa atttcattcc
     5821 gacaaaaata taaaaaacta cctattattt gttatttttc tatgagtact tttggaaaac
     5881 agctatagta tttggtcaca ggttttgaaa atgccctgga atttaattgt tctgaagatt
     5941 ctacaacatc ttccaccgtt ttatcctttt ttaaaatccg aatcggttca attactttgt
     6001 tgtatcctaa cgtttttatt ttacctttaa atacaaaata atctgtttct gctaatgaaa
     6061 tagggtaata ttccaaaagt ttatgtttgt aaagcttaat ttcttcttta tcaaattttt
     6121 cattactcac tttaattttg agtaaatcac gattaataat cattttactt aatgaactta
     6181 gtataaaatc agggtgatat tgccatgcct tcaaagcact cactatatca aaatcgtcta
     6241 actgagcgaa tttatctaaa attatggcat caaaatcagc aaaatcaact ttattttgta
     6301 aaaaaaactg taacggttca ctacatgcca aaaccgttcc tttctgtgtg agttctttcg
     6361 ctctttttaa tgttctggtc agaatgagtt ctgcaaccaa actggtttta tgtaaatatg
     6421 cctgccaata cattaatcgt ctggccatca gaaacttttc gatagaataa atagctttct
     6481 cttccattac taaaacatca tcaaccacat gaagcatttg aatcaaacga tcagaattga
     6541 tatttccttc cgccactcct gaataaaagc tgtcgcgttt caaatagtcc attctatcca
     6601 tatctaattg actggaaata agttgcaaca taaatttacg atgatattct cctttaaaaa
     6661 tttggattgc taaacttaat tttccatcaa aggctctatt caattgattc ataaaaagca
     6721 atgaaatttc ttcatggtgc acattttcta cgatactttg ctccatcgca tgggaaaagg
     6781 ggccatgacc tatatcgtga agcaaaatag caacataaag agccgtttct tcctcttttg
     6841 aaatcgaaat ttctttaaac cgtaaaactt ccactgcctt ttgcatcaaa tgcatcgcac
     6901 ctaaagcgtg gtgaaaacgt gtatgatttg ctcctgggta aactaaatag gacaatccca
     6961 tttgagtgat tcgtcgcaat cgttgaaaat aaggatgctg aatcaaatca aaaaccaatt
     7021 catttggaat ggtgataaaa ccgtaaatag ggtcgttaaa aatttttagt ttattcgttt
     7081 ggctcactta gataattatt aagacaacaa atatactgaa ttttaagatt gtgaaattca
     7141 ctaaacattt acaataactt taatacttcc tgcgttcgat aattgttaaa ttgcaccttt
     7201 aaaacaaaac cattttatgg acaaaataaa aattctttgg gtcgacgatg aaattgattt
     7261 gctcaaacca catattcttt ttttagaaac taaaaactat gctgttacaa cctgcaataa
     7321 tggtcgggat gctgtagata tttttgaaga aaatcaattt gacatcgttt ttttagatga
     7381 aaatatgccc ggaatgagtg gattggaaac cctttcagaa atgaaagaaa aaaaatcagc
     7441 cgttcccatt attatgatta ctaaaagtga agaagaaagc ataatggaag aagccatcgg
     7501 ttctaaaatt gccgattatt tgataaaacc tgtgaatcca aatcaaattt tgttggcttt
     7561 aaagaaaaac ttagaccatt ccagactaat ctctgaaaaa actactttag attaccaaaa
     7621 agagtttagg aaaatagcca tggatttagc cgtggttaat tcatttgaag attggaccga
     7681 attatataaa aaattgattt tttgggaaat tgaattagaa aatattgaag accaagggtt
     7741 gatttcaata ttagaatcac aaaaaacaga agccaatagt caatttggta aatttattga
     7801 aaaaaattat gaaaaatggt ttgaagataa agccgataag ccgattttgt ctcacaaatt
     7861 attcagagaa ggtgttgttc cagaactagt taaaaaagac aagcctattc tttttgtagt
     7921 gattgacaac ctacgatatg accaatggaa ggcctttgaa agtgttgtga acaaccatta
     7981 taaattagaa aaagaaatgg cttattttgc tatgcttcct accgcaactc aatatgccag
     8041 aaatgctatt ttctccggat taacgcctct agaaatggaa aaacaatttc cacaatactg
     8101 gcttaatgac gttgatgatg gtggtaaaaa tttgtttgaa ggtgaatttt tagccgctca
     8161 attgaaacga ttaggtttga atataaaaag tgagtattac aaaattacaa attccagaga
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atgaagaaaa agatgtttat
     8341 gctgtaaaag acccaaaaaa aataggttta ccaaccatca atatgagtag ttcgtttatt
     8401 tttgcaaaaa atgatttgtt cttagcttat gtcaataatt acaatcatta tgtgagttat
     8461 tacagaaatt cctatcaaca tggagggatt tctttggaag agatgattgt gccatttttg
     8521 gtcttcaatc cgaaataagt tggctatttt cgttttaccg ttggaaaggt gatatatttt
     8581 aacagctaat tgctattttg cagactattt taatttagaa ataaaataat ggaatttatt
     8641 tataatttga acgaaattga tgcaattgct caacaagtaa ttgctcaaaa tcctcagaaa
     8701 gttattcttt ttaatggttt aatgggcgtt ggaaaaacaa cttttataaa agctttatca
     8761 aagcgacttg gtgttgaaaa gccaacttca agtcccactt tttccttagt gaatgaatac
     8821 caagcaaaag agggtttggt ttatcatttt gatgtttatc gtttaaaaaa tgaaatggaa
     8881 gcactcgatt ttggaataga agattatctt tattccggca attggtgttt cattgaatgg
     8941 gcagaaaaaa taccaaatct aatacctgag aaacacaatg tcattactat agaaattttg
     9001 gataatggaa atcgaaaatt aattttagac taatttttat tttttaccat aaagccatgt
     9061 gctttttttg atttaacttt tttaatggca acaattttta tgtctactat tttttaaaat
     9121 ttatttttcg attaaaaata actcttttta attccaaaat atttcctaaa ttagaacttt
     9181 atttcaaacc taatttatgc ctctgactcc cttctccaaa caacaattac ttccgcagga
     9241 agaaaagttg gaaattgccc gacataaaag tgaattgttt attggcattc caaaagaaac
     9301 ctcctaccaa gaacgtagaa tttgtttgac tcccgatgcc gtaagttcat tagtttctca
     9361 tggacatcgt gttctattag aagccggagc cggagaaagt gccagttatt ctgataaaga
     9421 atatagtgat gccggtgctg aaattacgca agacccaaaa aaagttttca gttgtccgat
     9481 gattctaaaa gtcgaaccac ttacttctgc agaaatagaa atgataaata tgaatactat
     9541 tgtcatttca gctatacaat taaaaacaag aaaaaaagaa tatttcgaaa aattagccaa
     9601 aaagaaaatc acagcttttg cttttgaatt cattaaagat gatgatggtt cctaccctgc
     9661 tgtgaaatct ttaagtgaaa ttgcaggaac agcttcgata ttaattgctg ccgaattaat
     9721 gattaacaat caatttggaa aaggattact atttgggaat attacagggg ttgctcctac
     9781 cgatgttgtg ataattggag caggaacagt gggtgaattt gcagctaaaa cggccattgg
     9841 attaggggca aatgtcaaag tttttgataa ttcaattaca aaattgagac gtttacaaaa
     9901 taatttaaaa caaccgattt ttacttcaac catacaacca aaatccttat taaaagctct
     9961 tcgtcgttgt gatgttgcaa ttggtgctat tcgtggacaa aacagaacac ctgttgtggt
    10021 gactgaaact atggtagaac acatgaaaaa aggtgcagta attgttgacg taagtatcga
    10081 tacgggtgga tgtttcgaaa cctctgaaat aactacccat gaaaaaccaa cctttatcaa
    10141 aaataatgta gtgcattatt gtgttccgaa tattccgtca cgttattcta aaactgcttc
    10201 tttatcaata agcaatattt taacacccta tttaatccaa attgctgaag atggtggagt
    10261 agaaagtgcc attcgatgca atcgcggctt gaaaaacggt ttatattttt accatggtat
    10321 tttaaccaat aaatccattg gtgattggtt tgatttacca aatagtgata tcaatttgat
    10381 tgtgttttag ggttttgttc gtacttttgc accgttaaaa ttctctccta atgaagttct
    10441 accaacgttt cgcttttttt cttttcggat tattaattgg aatcattttc ttgatatatt
    10501 ttttaggagc taaagcaaaa tccagagggg tgactttttg ttatttgcca aattgtaggg
    10561 ttttaaaaga tatcagaagt aagccttttt tctattctga aaaatcatcg aaagttttag
    10621 cggaaagttg gattgatacg gtagacatca aaaacacgtt gacttatggt gaggtcgatt
    10681 ttagtaaaag taatatcaaa gaaggtgctg ccaaattata tatcatagaa ggcaagacta
    10741 taaatcaagt tccggttact ttagaagtat taaactacga atctaaagtg gttttaaaag
    10801 aaataaaaaa ataaaatatt atgcaacata ttattgatcg atttatcagc tatgtaacga
    10861 tagacacaga atctgatcca aattcggaaa caacaccaag ctcaaaaaaa caattagttt
    10921 tagctaaaaa tttagtgaaa gagctcaaaa caattggaat ggaagatgta accattgaca
    10981 aaaatggtta tgtgatggct acattgccaa gtaatgttga acatgaagtt ccgacgatag
    11041 gatttgtttc acattatgat acaactccgg attttatagg tgcaaatgtg aaaccacaaa
    11101 tcatagaaaa ttatgatgga aaagatattg tattgaatgc agaacaaaat ataatattat
    11161 ctccttccta ttttaaagac ttgttgcaat ataaaggtca aacccttatc actactgacg
    11221 gaactactct tttgggagct gatgacaaag ccgggattac cgaaattgtc actgcaatgg
    11281 aatacctaat caaaaatcct gaaatcaaac acggaaaaat acgggttggt tttacacccg
    11341 atgaagaaat tggtcgtgga gctcaccact ttgacgtaga aaaatttggt gctgaatggg
    11401 cttatacgat ggatggtagt caaattggtg aattagaata tgaaaatttc aatgctgccg
    11461 gtgccaaaat tactttcaaa ggaaaaagcg ttcatccggg ttatgcgaaa ggaaaaatga
    11521 ttaattcgat gcttttagcc tccaaattta tttcaaaatt accaaaaaaa gaagtgcctg
    11581 aagaaaccaa aggttacgaa ggattttttc atgtaaccgg aattacaggc agtattgaag
    11641 aaagcactgt ttcactgatt gttcgtgatc atgacaaaaa aatattcaaa aaaagaaaag
    11701 actttttaca tgaattgaca gccaaattca ataaaaaata ccaaaaacaa tttggcgaag
    11761 atatcgtaat tatcgaaatt aaggatcaat attttaacat gaaagaaaaa gtaaaacctg
    11821 tttttcatat tgttgaaatt gccgaaaaag cgatgaaaaa actagatatc aaaccaatta
    11881 taaaacctat tcgtggtgga acagacggtt gtcaattatc ctacaaaggt ttaccttgtc
    11941 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacttttaaa ctactttcaa
    12061 attctttttc ttgctttgct cttttttgtt gtggtcttat cataaaaaaa taaataacca
    12121 cgaacattaa taaaaatggt aataatgatt gcaaattttc catactattg atttactgtt
    12181 gtttgtgttt ctactggttt tacttttggc gtaacttctg cattaatggt taacacttct
    12241 cttgtggata aagtattagc aacaatcgtt actgattttt gttgtttgcc cggtttaccg
    12301 ttactgtcaa aagtaacttt cattttactt gattttccgg gttttattgg ttctttcgga
    12361 taatccggta cggtacagcc gcatgaacct gatgcatttg aaataatcaa atcagcatcg
    12421 ccggtatttg taaacgtaaa ataagtttct actttatctc cttcattaat taccccaaaa
    12481 ttatgttctt tttttccaaa tgtcattacc ggatattttc catctatcgg agctgaagct
    12541 gcttgttcag gtgtttgacc gttttcagta acgggttgaa caggcatcgg atttccattg
    12601 gcatcaactt gtttcgcttc ataattcatt ggaacatctg aacttgtagg atctactttt
    12661 gaaagtgcat cttctttttt acaggaagtt gttaaagcaa aagctaaaac agccatcaca
    12721 tacattgatt ttttaatcat aattctaaat tttaaatttt acatcaaacc tctaccggtt
    12781 ttttttaatt tatctgtttt ttgaaaatct ttaaccaaat tatctaaaat accattaata
    12841 aaaatactgc tttttggagt agcatattct ttagcaattt ccaaatactc attaatggtt
    12901 acttttattg gaatagatgg aaatttcaaa aattcacaaa ttgccatttt aagcataatt
    12961 gtatcaactt cagaaatacg ttccggatcc caatttggtg ttctgtcaat atattctttg
    13021 gctaaatcat tttcattcaa aactgttttt ctaaacaaat cgcgggcaaa atctttatct
    13081 tcagcatcct tatacaaagg caaaactgaa aaagattttt cattatgctc ttcaatatgc
    13141 tttaactgct ttaaaaggct agtgtttact aacggaatgt catcaaccca agtaattctg
    13201 aaatcttcta aataatcgta tagtttctcg ttagaagcta tcaattccgt aaaaatgtcg
    13261 gcaacaaatt ttttgtcggc ttcaaaagaa ctagaagtat ccgctaaata attttgataa
    13321 atggcacttc ttttaatttc atctaaaata atgagaatta aatcatcatt ttgagaccaa
    13381 ttattgattt tacggttttt taaagcaata gatattgaat tattatcttc taaaaattgt
    13441 aaaactttat tagcaataaa ttttctgttt ggctttaaat cttctgcagt agccaggtgt
    13501 ttttttgatg ctttttctaa aaagacttct tctttctttt taatttcaat caaagttgaa
    13561 acgacaatca aatataaatc ctgaatactt tctaagctgg aaaacaagaa tttctcttct
    13621 ttttccaaat cttcagaacc actttgatgc attgcataaa tgatttgcaa tactttaacg
    13681 cgaatatgtc ttctatttat caccctgtaa gaacttttta aaaaattagt gacgccccta
    13741 agagcgtcac aaaaatagta ctattttgtc ggaaacaaat tatttaccgc tttctttttt
    13801 acgttggtca attctatttt gagcaattgt taaggccgct tggtgtgttg taatattatt
    13861 ttttacagca taatcaaaaa tttctaacgt agtatggtag atgttttctg ttcttctcat
    13921 tgattccgct ttgtcataat gttcaatttc agcatataca ttgatgatac ctccggcatt
    13981 aattaagaaa tcaggagcat ataaaattcc tctctcttgc aaaatttttc cgtgaacatt
    14041 ttcattggct aattgattat tggcagctcc ggcaacaatt gatgcattca atttatatac
    14101 tgtatcatca ttaatggttg ctcctaatgc acaaggagca taaatatcaa catctgcact
    14161 atataaatca gaacctgtga aaacttttgc accatactgt gctcccactt cattcaatcg
    14221 atttatgtta atatcagcaa ttgtgacaat ggcaccttct ttcgttaaat aatcaactaa
    14281 agtttcacct acatggccaa ttccttgtac caaaactttt ttaccggcta aagaatcaga
    14341 accatatttg tatttagcag ccgctttcat tcccatgtaa acaccaaaag ctgttaccgg
    14401 tgatggattt cctgaaccac cacgagattc tgatattccg gttacataag gagtaacatc
    14461 tcgcaccgta tccatgtctt cggtttgcat acctacatct tctgcagtaa tatattttcc
    14521 gcttaacgaa tgaacaaatt caccaaattt acgcattaat tcaggcgttt tttgtgtttt
    14581 agcatcccca ataatgactg cttttccacc accaaggttc aatccagtaa tagcagattt
    14641 ataggtcatt ccacgtgata aacgcaaaac gtcgttcaat gcttcccact cattcgtgta
    14701 attccacatt ctagttcctc ctaatgcagg tcctaaaact gtgttatgaa ttccaattat
    14761 tgctttcaaa ccagtatctt tgtcgttgca aaaaacaatt tgctcgtgat tatcgaagga
    14821 tacttgaccg aaaaccggat ccattttttg aagttcttta ggagttgtta cttctgctat
    14881 catagttaaa aatttaaaag tataaattat gattttgtca aaaagacaca gcaaaaatag
    14941 ggttaaattc aaaagaatgc aatataatat aataatttta gcaatttgtt aatcttttgc
    15001 atgaattcaa tacaatacaa gaatagaata attctcattt ttttaacatt tttaaatcac
    15061 tagatacgtt ttatatcgat ttacaatgaa agaacttcac tatttaaata aatatttcat
    15121 caaatataaa taccgttttt tattaggtat tttaattact attgtagctc agatattttc
    15181 cctttttacg ccaaaattaa ttagtaaatc gttaaatatc attgaagaat atattaaaaa
    15241 tccaatcaag gatagcagtt taatcaaaca agaattactg ctcagtctag gtttaatcat
    15301 tggagcaacc attattgctg gctttttgac atttttaatg cgacaaactt taattgtgat
    15361 gtctcgctat atagaatttg atttaaaaaa tgaagttttt caacattacg aaagattatc
    15421 tcaaagtttt tacaaacaaa acagaacggg agatttaatg aatcgaatat cggaagatgt
    15481 tggcaaagtt agaatgtacg tgggccctgc agttatgtat accatcaata cctttatacg
    15541 ttttgccatc gttattctat atatgtacaa cgtttcgcca aaattgacgc tgtatacttt
    15601 gttgcctttg ccattattat cgtattgtat tttcaaatta agttcagaaa tcaataaaag
    15661 aagtacaata tatcaagaga atttatccaa tttatcgagt ttttctcaag aaactttctc
    15721 cggaattcga gttataaaag cctacgcctt agaaggacaa aacaaaaaag catttaacaa
    15781 tttatcccaa gaaagtcgtg aaaagaacat gcgtttagct gttgttaacg ctttatttgg
    15841 accattgatg attttattaa ttggtttaag caatttagtg gttatctatt ttggcggcat
    15901 gatgtatatt gagggtacaa ttcctaacat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn caaaaacagc gtgtctctat tgcccgggca attatcaaag atcccgaaat
    16081 cttgcttttt gacgattgct tatcagctgt tgatactgaa accgaagagc aaattttgaa
    16141 taatttattt gaaatttgta aaaacaaaac cactgtaatt gtgagtcatc gtgtatcttc
    16201 tgcaaagaat gcagacaaaa ttattattct tgaggaaggc gaaattttac aacaaggcac
    16261 tcattctcaa ttagtaaatc aagaaggtta ctataaggaa ttgtatctga aacaactttc
    16321 tgaaaaagaa ttgtcataat tttttgataa ttcaattatt ttttagattt ttggttacaa
    16381 cttacaacac ttaatttgac agaaagatta tgagagaaaa tgaaatgtta gaaaaagatg
    16441 agattttttc taaagtttta agagcaggaa gaagaactta tttcttcgat gttagagcta
    16501 ctaaagccga tgattactac atcacaatta ctgaaagtaa aaaatttacg gaggaagatg
    16561 gttcgtttca ctttaaaaaa cacaaaattt atttatacaa agaagatttt gtagctttta
    16621 aagaaatttt agatgatatg acttcttacg ttttagacca caaaggagaa gaagtaattt
    16681 cagaaagaca tcaaaaagat ttcaaaaaag aatatgctgt tgatgcagat gaaaccgaag
    16741 attctccgaa agcaagtttt acagacatcg attttgatga tatttaaaaa agcaaatccc
    16801 ttctgaaaag aggggatttt ttttaaccaa ttcgcaaacc atttgctact tttacatcag
    16861 aagttaaaag tacaatatca ttctcctctc ctactgagcc taaaactaaa cattcactat
    16921 taaattttcc aatctgtttt ttaggaaaat taattacggc aacaatctgt tttcctatca
    16981 aaacatcttt agaataacgt ttagtaattt gtgctgaagt ttttcgaatt ccaatttcat
    17041 ccccaaaatc aatgattaat tgataagccg gttttctggc ttccggaaaa tcattcactt
    17101 ctaaaatggt tccaatacgc atttcgactt tttcaaattc ttcccaagta agcatcctat
    17161 ttattcttaa aaatttaaag ttaaaaatag gaatacttcc tcaaaatttc ataactttaa
    17221 gcaataactt tttattaaac ttttttaatg gccaggacag catctaaaat gagttcatta
    17281 ggaaccttag caccaaattt tctattaaaa gacactaatt caggtaactt ttttaccttt
    17341 tctgattgca aaggagaaaa aggaaccttg gtatttttta tttgtaatca ttgtcctttt
    17401 gttcatcatg taatggaaga aattattatg gttgccaatg attatagagt gcaaggtatc
    17461 ggtattgttg ccatttcaag taatgatgtc gttcaatatc cacaagatag tccggaatta
    17521 atgaccgaat ttgcctttga aaataaattt gagtttcctt atttatatga tgaaacacaa
    17581 gaggttgcca aggcttttga tgccgcatgc actcctgatt tttttctatt tgacaatcaa
    17641 gacaaattag tatatcgtgg gcaattagat gactctcgtc ccggaaacgg aattccgctt
    17701 agtggaagcg atttaagggg agctatagat agtttattat ataatcgcat catcaatccg
    17761 aatcaaaaac caagtattgg ttgtaatatt aaatggaaaa aataaatgga aatagttcat
    17821 attagtgccg aatgttatcc ggttgccaaa gtaggcggtc tagccgatgt ggttggagct
    17881 ttgccaaaat atctaacaaa attaggccat ttaaccaaag ttgttgtccc tggatatgat
    17941 accaaatttt cgagagaaaa tgaatttgtt tcggttcatc aaggattatt aaaattaggc
    18001 tggttccaat tttcctatac cgttttcaaa gaaaaaacaa ataaattagg tttcgaatta
    18061 tatttaattg cgattcctga attgtttgat cgaccaaatg tgtattccta tgaagatgac
    18121 accgagcgat ttactgcttt tcaaatcgct tttttaaatt ggatgactga aaccaatcaa
    18181 attcctgata tcattcattg ccatgatcat cacaccggat taattccgtt tatgatgtca
    18241 catgccccaa tttatcaaca cttaaatcgt gttccaacgc tattaaccat tcacaatgga
    18301 caatatcagg gatggtttgg ttttgataaa ttgcattatt taccaaaagt aagttcctct
    18361 cgtttgggct ttttagaatg gaacggaatg ataaatccgc tggcttctgc cattaaatgt
    18421 gcttggaaag taacaacagt ttctcccagc tatttagatg aattaagtga aaaagcaaat
    18481 ggattagaaa atcttttacg atttgaacgt cctaaatgtc tcggaatact aaatggaatt
    18541 gattctgagg tttggaatcc ggataccgat gaaatgttag aaaacaagta ttcaataaaa
    18601 aatgtaaaag ccggaaaagc taaaaataaa aaattccttt gtgagcaatt taatttggat
    18661 ccctctaaac ctctttttac ttttatcgga cgattggtgg gagaaaaggg agccgattta
    18721 ttacctgaaa tttgtagtat agctttaaac cgaaacccaa atgaaattaa tattttaatt
    18781 ttaggttccg gtgatccaaa tgtagaacat ggtttaacac aattaaccca tttttacaat
    18841 ggaaattaca atacttatat tggttataat gaaaatttag cccatagaat ttatgctgga
    18901 gcagattatt tgctcatgcc gtcaagggtt gaaccttgtg gactaaatca actttatgcg
    18961 ttacgatatg gcactattcc tattgttaga agaacaggtg ggctcaaaga tactgttctc
    19021 gatataggcg atggtggatt cggaatttgc catgatcata ctagtgttta cgatgtgaat
    19081 cattctataa ctagagcaat tgatttgttt aacgatacca aacaatttga ttcaatccga
    19141 aaaataatta tgcaaataga ccattcatgg gataaagttg cacaagaata tgttgaagtt
    19201 taccaatcat taaaaaataa ttaaaatgaa aaaaagtgta ttagcaatca ttttaggagg
    19261 aggacaaggt tcaagattat atccattaac tgaaagccgt tcaaaacctg ctgtgcctat
    19321 tgccggaaaa tatcgtttgg ttgatattcc tatttcaaat tgtatcaatt cagatatcaa
    19381 acgaatgttt gttttgaccc aatttaactc cgcttcatta aatcaacaca tcaaaaacac
    19441 ctatcatttc agctatttca gcactgcctt tgtagatatt ttggctgctg aacaaacgcc
    19501 agataatcct acttggttcc aaggaacggc ggatgccgtt cgacaatgca tgcaacattt
    19561 tttgaatcat gaatttgatt atgctttaat attatccggt gatcaattgt atcaaatgga
    19621 ttttaatttg atgattgaag ctcatcaaaa aagcggagca aacataacta ttgcaacact
    19681 tcctgtttca gcaaaagaag ctactgattt tggaatttta aaaaccgatg aaaatagttt
    19741 gataacttct tttattgaaa aacctgctac tgatttgttg ccgcaatgga cctccgaggt
    19801 aagtgatgaa atgaaaaaag agggcaaaca ttatttagcc tcaatgggta tttatatttt
    19861 taatagagat ttactgattg aattaatgtc aaatccaact actaaagatt ttggtaaaga
    19921 aattattccg caagcaatcg gaaatcaaaa aatattaagt taccaatatg agggatattg
    19981 gacggacatt ggaaatattg attcattttt tgaagctaat ttaggattaa ctgattccat
    20041 tccgaagttt aatttatttg acaattctag taaaatttat acccgtgccc gcgttttgcc
    20101 tccaagtaaa atattaggca aaacgtatat tgaaaaatca atggtagccg aaggttgtat
    20161 tcttaattct gcaaaaattg agcattccgt cattggtatc agagcaagag ttgacgaaga
    20221 tactacgatt caaaactcct atgtaatggg tagcgatttc tatcaaaatt tagatgaaat
    20281 cagtcataat aatgaaaata atatcatcaa tataggtatt ggaaaacgtt gttttatcaa
    20341 taatacaatt gtcgataaaa attgtagaat tggtaacgat gtttatctaa atggtggtca
    20401 ccatctggaa gatgccaata ctccatttta caccattaaa gaaggcatta ttgttgtaaa
    20461 aaaaggtgct actttaccgg atggatttag attagaataa ctttttgtta ctgattcatg
    20521 gatttataac tgtgaatcag taactttttt tctttaaaat aacatcaacc ggtctgttgg
    20581 tcctgggtcc aaattgttgt gcttgccgtt cattttcgga tgaacataaa atgtacatgt
    20641 tatagggaga aagcgggata atttgtgttt caaaataacc accgttttct tccatatcaa
    20701 tatcttcaat ttttacacta aaaatgtctg ccgtgaatgt attgacgaca gaaagcaaat
    20761 aataatcttt agcaaaagga aaaaaccatt ttccttccgg atttgaattt tccactttat
    20821 tattatttct gtctaattta taattaggag taaaaagcaa tggtttatta ttatttaccc
    20881 aactccaacg gttattttta tttattacat cgctcccact actatcgact attgtgattt
    20941 ttaggttttt gatgttttca gttttccctt gttcatgcac atttattact aaatacgatg
    21001 taaagtcata accacaatgt tgtacattaa tgacttgaga ttgagctgta taataaaata
    21061 aaataaaaaa tagaaataaa aaacgcataa ctgaaaactg attttgtaaa tatacgaaat
    21121 caatagttat tccaaaaaag aaagcattct tttcagaatg cttcttttat atttgaatga
    21181 attgagtttt atgaattttc aaggattttc aacctttgcc tttcacctat catccattag
    21241 ctacaatcta tttcacatcc attaattcta catcaaaaat caatgtagca tccggtggaa
    21301 taacacctcc cgcaccgcga gaaccatatc caaggaacga aggaataaca aaacgggctt
    21361 tgtctcccac ttgtaataac tgaatacctt catcccaacc ttcaattacg tgaccttttc
    21421 ctaaagggaa atcaatcggc tgttttcttt tataggatga atcgaaaacc actccgtttt
    21481 ctaaagaacc tgaataatga accgaaacag ttttgccttt ttcagcttgt tttccatttc
    21541 ctttttgaat gattttgtaa cgtaagccac tttctgtctt ttcaaatccg gcagctaatt
    21601 tttccaaagc ttcttccgct tttgcttttt cttccgctat tcttttttcc cgagaacctt
    21661 caaatgttct gaaagtttca atagcattcc atttttgagc ttcgtcacct actcgaataa
    21721 tttccaatgt ttccaaaaca tcgccttgag caatagcatc aacaacatct tgaccttcca
    21781 ctacatttcc aaaaacggtg tgttttttat ctaaccaagg agttgcaacg tgcgtaataa
    21841 aaaattgtga tccgttagaa cccggacctg aatttgccat cgataaaaca cctggtccgt
    21901 catgacgtaa atccggatga aactcatcgt caaattgata acccggacca ccggttccta
    21961 atccttgagg acaaccacct tgaatcataa aatcagcaat tacacggtga aattttaaac
    22021 cgtcgtaata cggtttcccc attggttttg cttcgttttc taattgacct tcagccaaac
    22081 ctacaaagtt tccaaccgtt cccggtgttt tatcgtgtgt taatttgact aaaattggac
    22141 ctttagttgt attgaattta gcgtaaattc cgttttccat gttttcactt tttaaaatgt
    22201 gttgcaaatt taatacatta ttgtcgatta accttaaaat tgtacttttg aatctatcaa
    22261 taaatataac aatggaatca ctttttgaca aatctgataa tgccgcaatt attcaacgaa
    22321 ttcattcttt aaattcagac agcaaaccct tgtggggaaa aatgaatgtc gctcaaatgc
    22381 tctcgcattg tcaggctccc cttgatgttg ctactgggga cttacaatta aaatctaatt
    22441 tcattttgca attcataggt aaaatatata aaaagaaaat acttgccgct cccggattta
    22501 aaaaaaacag tccaactgct cccgatttct tacgaaataa tccggtagat tttgaacagg
    22561 caaaattgga attgattgaa aaagttttaa aattttcaac cctcggacca aaagtaattg
    22621 ccaaccacaa gcatccattt tttggtgaaa tgaccaatga agaatgggat ctattacaat
    22681 ggaaacactt aaaccatcat ttaaatcaat ttagcgtata aaatggaaaa tctgcccgat
    22741 tatatcaaca tcaacaagga aacttggaat aagaaaacag ctgttcatat agcatctgat
    22801 ttttataata acgaagcctt cttaaatgga aaatcatcat taaaccattt tgaaattgat
    22861 ttattaggtg atgtttctca aaaaaagatt ttacatctac aatgtcattt tggacaagac
    22921 acactctctt tagctagatt aggggcaaaa gttaccggta ttgatttttc tgatcaagcg
    22981 attgaaaaag ctaaagaata ttcgaaacaa cttgatttag atgccacttt tatctgttgc
    23041 gatgtatatg acacacctaa ttatttagac gaaaagtttg atattgtttt tacaagctac
    23101 ggcactatcg gttggttacc cgatatggat aaatgggcgg ctgttgtttc tcacttttta
    23161 aagcctgaag gtaaatttgt ttttgttgat tttcatccgg ttgtttggat gtttgatgat
    23221 gatttcaaag aagtatttta taattatttt aaatcggaac ccattataga agaagaaaca
    23281 ggcacttatg cggataaaaa ggcttccttg attcaaaaaa ctatttcatg gaatcactcc
    23341 atttcagaag ttgtaaatgc attgattgaa aatggtttaa ccatccatt
//
LOCUS       DAOTCW010000022        22375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1221_length_22375_cov_43.424955, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000022 DAOTCW010000000
VERSION     DAOTCW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 22375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22375
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1221_length_22375_cov_43.424955"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(445..756)
                     /locus_tag="P5335_03245"
     CDS             complement(445..756)
                     /locus_tag="P5335_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRZ73931.1"
                     /translation="MSDNNQQQGQINIELDEKVAEGIYSNLAIINHSASEFVLDFVTL
                     MPGVPKAKVKSRIILTPQHAKRLLKAIGENIHRFEKAHGEIKDSDQPQVPLNFGSAGQ
                     A"
     gene            complement(762..5069)
                     /gene="rpoC"
                     /locus_tag="P5335_03250"
     CDS             complement(762..5069)
                     /gene="rpoC"
                     /locus_tag="P5335_03250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRZ73932.1"
                     /translation="MMNNRNNKDKNPIKRFNKISIGLASPESMLAESRGEVLKPETIN
                     YRTHKPERDGLFCERIFGPVKDYECACGKYKRIRYKGIVCDRCGVEVTEKKVRRDRVG
                     HINLVVPIAHIWYFRSLPNKIGYILGLPSKKLDMIIYYERYVVIQAGIAQNAEGDSLS
                     RLDFLTEEEYLNILDTLPMENQYLDDNDPNKFIAKMGAECIMDLLARTNLDQLSYDLR
                     HAANHETSKQRKTEALKRLQVVESFREANLNRENLPQWMILKVIPVIPPELRPLVPLD
                     GGRFATSDLNDLYRRVIIRNNRLKRLMEIKAPEVILRNEKRMLQEAVDSLFDNTRKAS
                     AVKTESNRPLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKLFECGIPKDM
                     AAELYKPFVIRKLIERGIVKTVKSAKKIIDKKEPVVWDILENVIKGHPVLLNRAPTLH
                     RLGIQAFQPKLIEGKAIQLHPLVCTAFNADFDGDQMAVHLPLGPEAILEAQLLMLASH
                     NILNPANGAPITVPSQDMVLGLYYMTKERLSTPEHKILGEGLTFYSAEEVNIALNEGK
                     LELNARVKIRARDFDETGKLVYKLIQTTAGRVLFNEVVPEAAGYINEVLTKKSLRDII
                     GRILSVTNVPTTAAFLDNMKDMGYKFAFRGGLSFSLGDIIIPHQKQELIADAREEVEA
                     ITMNYNMGLITNNERYNQVIDVWTSKNALLTELAMKNIREDQQGFNSVYMMLDSGARG
                     SKEQIRQLTGMRGLMAKPKKSTAGGGEIIENPILSNFKEGLSILEYFISTHGARKGLA
                     DTALKTADAGYLTRRLHDVSQDVIVNTEDCGTLRGIEVSALKKNEEIVESLGERILGR
                     VALQDVINPLNLEVLIEGGQEITESVMRAIEASPIERVEVRSPLTCEAAKGICAKCYG
                     RNLATGRMTQKGEAVGVIAAQSIGEPGTQLTLRTFHVGGVAGGMSEESNIITRFKGRL
                     ELDDLKTVKGEDADGKAIDIVISRSTELKLVDENTGIVLNTHNIPYGSTIYVKDGQIV
                     DKGTTICKWDPYNGVIISEFTGKIAYEDLEQGQSFQVEIDEQTGFQEKVISEGRNKKL
                     IPTLLIYGKDGELIRSYNLPVGAHLMVDDGEKIKAGKILVKIPRRSSKTGDITGGLPR
                     ITELLEARNPSNPAVVSEIDGVVSFGKIKRGNREIIIESKFGEVKKYLVKLSNQILVQ
                     ENDFVKAGMSLSDGAITPEDILRIQGPSAVQQYLVNEIQEVYRLQGVKINDKHFEVVI
                     RQMMRKVRVEDPGDTLFLEEQLAHTKDFIIENDKLFGMKVVEDAGDSQNLKPGQMITP
                     RELRDENSLLKREDKQLVSARDVITATATPVLQGITRASLQTKSFISAASFQETTKVL
                     NEAAVAGKVDFLEGLKENVIVGHRIPAGTGMREYDNNIVGSKEDYNEIMAAKEEYNY"
     gene            complement(5154..>6293)
                     /gene="rpoB"
                     /locus_tag="P5335_03255"
     CDS             complement(5154..>6293)
                     /gene="rpoB"
                     /locus_tag="P5335_03255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137817.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRZ73933.1"
                     /translation="VVLDKKLFARAVKDKRKRTKDKDDLASLEMEFEVKFNELKEKLV
                     EKLFNIVNGKTSQGVMNDLGEEVLPKGKKYTQKMLNAVEDFAHLTKGQWVSDDETNFM
                     VNDLIHNYKIKLNDLQGALRREKFTITVGDELPAGILKLAKVYIAKKRKLKVGDKMAG
                     RHGNKGIVARIVRDEDMPFLEDGTPVDIVLNPLGVPSRMNIGQIYETVLGWAGKNLGR
                     KYATPIFDGATIDQINELTDEAGIPRFGHTYLYDGGTGERFHQAATVGVIYMLKLGHM
                     IDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGASSTLREILTVKSD
                     DVIGRAKTYEAIVKGEPMPEPGLPESFNVLMHELKGLGLDIRLEE"
     assembly_gap    6294..6393
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6394..8348)
                     /gene="rpoB"
                     /locus_tag="P5335_03260"
     CDS             complement(<6394..8348)
                     /gene="rpoB"
                     /locus_tag="P5335_03260"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRZ73934.1"
                     /translation="MITNQTERLNFASTKNIPQYPDFLDIQVKSFKDFFQLETKSDER
                     GNEGLYNTFMENFPITDTRNNFVLEFLDYFVDPPRYSISECIERGLTYSVPLKARLKL
                     YCTDPEHEDFETIVQDVYLGTIPYMTPSGTFVINGAERVVVSQLHRSPGVFFGQSFHA
                     NGTKLYSARVIPFKGSWIEFATDINNVMYAYIDRKKKLPVTTLFRAIGFERDKDILEI
                     FDLAEEIKVSKTGLKKYIGRKLAARVLNTWHEDFVDEDTGEVVSIERNEIILDRDTII
                     DKDNVEEIIDSNVKSILLHKEDNNQADYTIIHNTLQKDPTNSEKEAVEHIYRQLRNAE
                     PPDEDTARGIIDKLFFSDQRYNLGEVGRYRMNKKLGLDIPMEKQVLTKEDIITIVKYL
                     IELINSKAEIDDIDHLSNRRVRTVGEQLSAQFGVGLARMARTIRERMNVRDNEVFTPI
                     DLINAKTLSSVINSFFGTNQLSQFMDQTNPLGEITHKRRLSALGPGGLSRERAGFEVR
                     DVHYTHYGRLCPIETPEGPNIGLISSLGVYAKVNGMGFIETPYRKVTNGVVDLISEPV
                     YLSAEEEEGKMIAQANIEMDGKGKILADKVIAREEGDFPVVDPSAVHYTDVAPNQIAS
                     ISASLIPFLEHDDANRALMGSNMMRQAV"
     gene            complement(8614..8991)
                     /gene="rplL"
                     /locus_tag="P5335_03265"
     CDS             complement(8614..8991)
                     /gene="rplL"
                     /locus_tag="P5335_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRZ73935.1"
                     /translation="MADLKQFAEQLVNLTVKEVNELATILKDEYGIEPAAAAVVAGPA
                     AGGGDAAVVEEQTEFTVVLKEAGASKLAVVKAVKELTGLGLKEAKDLVDAAPSNVKEG
                     VTKDEAEGLKKSLEEAGAVVELK"
     gene            complement(9054..9569)
                     /gene="rplJ"
                     /locus_tag="P5335_03270"
     CDS             complement(9054..9569)
                     /gene="rplJ"
                     /locus_tag="P5335_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009392540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRZ73936.1"
                     /translation="MTREEKSRVIEDLTAQLADSNVVYIADISNLNAETTSNLRRACF
                     KAGIQLNVVKNTLLEKAMEASANDYGDLPSVLKGNTSIMIAENGNAPAKIIKEFRKKS
                     DRPLLKGAYIHQAIFIGDNQLDALVSLKSKEEVIGEIIGLLQSPAKNVVSALKSGGGK
                     IAGIVKTLSER"
     gene            complement(9592..10281)
                     /gene="rplA"
                     /locus_tag="P5335_03275"
     CDS             complement(9592..10281)
                     /gene="rplA"
                     /locus_tag="P5335_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRZ73937.1"
                     /translation="MAKLTKKQKEAASKIEKSKLYSLKDASALLKDVASAKFDESVDI
                     AVRLGVDPRKANQMVRGVVTLPHGTGKDVRVLALVTPDKEAEAKAAGADYVGLDDYLQ
                     KIKDGWTDVDVIITMPAVMGKLGPLGRILGPRGLMPNPKTGTVTMDVAKAVQEVKAGK
                     IDFKVDKTGIVHAGIGRISFNADMIVDNAHEIIQTLIKMKPTAAKGTYIKSIHLSSTM
                     SPAIALDPKAV"
     gene            complement(10295..10732)
                     /gene="rplK"
                     /locus_tag="P5335_03280"
     CDS             complement(10295..10732)
                     /gene="rplK"
                     /locus_tag="P5335_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRZ73938.1"
                     /translation="MAKEISKVVKLQVKGGAANPSPPVGPALGAAGVNIMEFCKQFNA
                     RTQDKPGKVLPVQITVYKDKSFDFVVKTPPAAIQLMDATKLKSGSGQPNRKKVASVTW
                     DQIRTIAEDKMVDLNAFTIEAAMSMIAGTARSMGITVSGVAPF"
     gene            complement(10798..11349)
                     /gene="nusG"
                     /locus_tag="P5335_03285"
     CDS             complement(10798..11349)
                     /gene="nusG"
                     /locus_tag="P5335_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRZ73939.1"
                     /translation="MADSSLKKWYVVRAVSGQENKVKTYIDNEINRLHLSDFIAQVLV
                     PTEKVVQVRDGKKIAKDKVYFPGYVMIEANLTGEIPHIIKSITGVIGFLGEVKGGDPV
                     PLRLSEVNRMLGKVDELAINTDSQNIPFNIGETIKVIDGPFNGFNGTVEKINEDKRKL
                     EVMVKIFGRKTPLELGFMQVEKV"
     gene            complement(11358..11555)
                     /gene="secE"
                     /locus_tag="P5335_03290"
     CDS             complement(11358..11555)
                     /gene="secE"
                     /locus_tag="P5335_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRZ73940.1"
                     /translation="MTKVVNYITDAFEELKSNVTWLPWSEVQRLTIIVAIFTVVFALA
                     TWGVDEVFRKAISGFFNLIKG"
     gene            complement(11571..11641)
                     /locus_tag="P5335_03295"
     tRNA            complement(11571..11641)
                     /locus_tag="P5335_03295"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11607..11609),aa:Trp,seq:cca)
     gene            complement(11699..12886)
                     /gene="tuf"
                     /locus_tag="P5335_03300"
     CDS             complement(11699..12886)
                     /gene="tuf"
                     /locus_tag="P5335_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRZ73941.1"
                     /translation="MAKENFNRNKPHLNIGTIGHVDHGKTTLTAAITKVLSDAGYCQA
                     KSFDQIDNAPEEKERGITINTSHVEYETANRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGIPRIVVFMNKVDMVDDAELLELVEMEIRDLLSF
                     YDYDGDNSPVIQGSALGGLNNDPAWTPKILELMEAVDNWIQEPERDIAKPFLMPVEDV
                     FTITGRGTVATGRIETGICNTGDPVEIIGMGAEKLTSTVTGIEMFRQILDRGEAGDNA
                     GILLRGVAKEDIKRGMVICKPGSVKPHAKFKAEVYILKKEEGGRHTPFHNNYRPQFYV
                     RTTDVTGTISLPAGVEMVMPGDNLTIEVTLLSAIALNVGLRFAIREGGRTVGAGQVTE
                     ILD"
     gene            complement(12939..13010)
                     /locus_tag="P5335_03305"
     tRNA            complement(12939..13010)
                     /locus_tag="P5335_03305"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12976..12978),aa:Thr,seq:ggt)
     gene            complement(13091..13171)
                     /locus_tag="P5335_03310"
     tRNA            complement(13091..13171)
                     /locus_tag="P5335_03310"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13135..13137),aa:Tyr,seq:gta)
     gene            complement(13259..13332)
                     /locus_tag="P5335_03315"
     tRNA            complement(13259..13332)
                     /locus_tag="P5335_03315"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13296..13298),aa:Thr,seq:tgt)
     gene            complement(13424..13726)
                     /gene="raiA"
                     /locus_tag="P5335_03320"
     CDS             complement(13424..13726)
                     /gene="raiA"
                     /locus_tag="P5335_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRZ73942.1"
                     /translation="MKVNVNAVNFNVDRKLVDFVTERMDKLEKYYDKVVVVDVFLKVE
                     ATSEKENKIVEIKVHVPGDDLIVKKQCKTFEEAVDSSSEALERLLLKRKEKIRTHI"
     gene            complement(13757..14653)
                     /locus_tag="P5335_03325"
     CDS             complement(13757..14653)
                     /locus_tag="P5335_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRZ73943.1"
                     /translation="MQSNQQAFKDYLQMQKNYSLHTVKAYVKDIEEFDSYNKIHFEQD
                     SIDIVGYSQIRSWIVSLVDGNISASSVNRKISSLKSYYKFLLRIKQIEQSPLLKHKAL
                     KTPKKIQIPFSEKEVDLVLNQIIYPDGFEGIRDKLIIDMFYTTGIRRAELINLKLSSI
                     DINSNTLKVIGKRNKERILPLLPIIISQIKMYIIQRNAIQDIKNTEQLFLTQKGVKLN
                     DSLVYRLINTYFSTISEKVKKSPHILRHTFATHLLNNGADLNSVKELLGHSSLASTQV
                     YTHSSLSELKKVYSEAHPRNQK"
     gene            complement(14722..14916)
                     /gene="rpsU"
                     /locus_tag="P5335_03330"
     CDS             complement(14722..14916)
                     /gene="rpsU"
                     /locus_tag="P5335_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRZ73944.1"
                     /translation="MLIIPIKDGENIDRALKRYKRKFDKTGTVRQLRARQAFIKPSVV
                     KRAQVQKAQYIQTLRDSVEM"
     gene            complement(14989..16158)
                     /locus_tag="P5335_03335"
     CDS             complement(14989..16158)
                     /locus_tag="P5335_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRZ73945.1"
                     /translation="MNSIYFTEEHQLFRVTLRDFLHKEVVPHIEKWEKTGTIERFIWK
                     KFGEMGFFGINYPEAYGGMNLDLFYTVIFLEELQKIKSSGFAAAMWAHAYLAMTHLNA
                     EGDEQIKQDYLVPSISGDKIGALCITEPFGGSDVAGMRTSAIKKGDKYYLNGSKTFIT
                     NGVYADYYVVAAKTSPELGNKGISIFLVDTSNPGISATKLDKLGWKASDTAEIAFDNV
                     EISEKNLMGEEGKGFPYIMQHFALERLIMAINAHARSEYAIDYTIEYMSQREAFGSTI
                     NKFQALRHTMVEHATDVEHCKVFNYAAVARLNKGEYVVKEATMAKLKSTKVADETIYS
                     CLQMLGGYGYMEEYPLARLSRDSRLGPIGGGTSEILKEILSKMIIDKQNYQPAVK"
     gene            complement(16193..17062)
                     /locus_tag="P5335_03340"
     CDS             complement(16193..17062)
                     /locus_tag="P5335_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRZ73946.1"
                     /translation="MKIKSYFQFTKEQRRGVLSLLLLIVIIQLSYFYWSSSKTTKPST
                     SDKSWLAMQSVIDSLKISQSSTERKIYPFNPNFISDYKGYQLGMSLEEINRLFEFRKQ
                     NKYVNSVQEFQQVTGVSDSLLLAISPYFKFPDWVNQPKKAFSSFAKNTYENKKSIVRK
                     DINEATQDDLIKIYGIGPALSERILKQKDILGGFVTMEQMESIWGLSPEVIENLNKYF
                     QVTTIPTVNKIDVNNLSVKELAKFPYFNYTIAKEIVTYRSMNNGIHSFEDLTKIKGMP
                     NEKIKIIALYLEF"
     gene            complement(17080..18576)
                     /locus_tag="P5335_03345"
     CDS             complement(17080..18576)
                     /locus_tag="P5335_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013304455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HRZ73947.1"
                     /translation="MKKLFSLFALLLPILTFSQEKGLDEKINDVIKPAVDAMASVFFY
                     KPFASFGFDMPLVVLWLVVGATFFTIYMGFINLKGIKHAYQLIRGDYDKPGDAGEVSH
                     FQALVTALSGTVGLGNIAGVAVAISVGGAGATFWMILAGFLGMSSKFVECTLGVKYRR
                     LNDLGEVSGGPMYYLSEGLKRKGYAGFGKVLAVVFSILAIGGSFGGGNMFQANQSFAQ
                     LANVFPVFIGNGFWYGLVVAFFVGIVIIGGIKKIASVTDKIVPFMVGLYVITSLIIIF
                     VNFAHIGDAFSEIFSGAFTADGISGGVIGVLVVGFQRAAFSNEAGVGSAAIAHSAVKT
                     DEPVSEGIVSLLEPFVDTIVVCTMTALVLIFSGYAQDTQGLTGSALTSAAFSSVLPWF
                     KYVLLVAIILFAFSTMISWSYYGMKSWTYLFGNTKKMENVYKILFLVFVVVGSSASLG
                     AVIDFSDMMILGMAFPNILGLYFLSSEVKADLKEYFRKLKSGEIKKFE"
     gene            complement(18666..18893)
                     /locus_tag="P5335_03350"
     CDS             complement(18666..18893)
                     /locus_tag="P5335_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRZ73948.1"
                     /translation="MSWITSIRHFFEKHGFHVSSRLADRLGMRASNVRLFFIYISFVT
                     VGLWFGVYLTLAFWLRLKDMLHTKRSSVFDL"
     gene            complement(18893..19315)
                     /locus_tag="P5335_03355"
     CDS             complement(18893..19315)
                     /locus_tag="P5335_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HRZ73949.1"
                     /translation="METNNSSKPSFSQKVKKYFYRFLFLFLLSFVGYFLFIMYVPYSE
                     GVRSGELIKFSKKGVVFKTWEGEMSQGISGAQIFSFSVANKEKEVIQQLEDLQGKYVK
                     LKYEERYRTFFWWGDSPYFVTNVEEETTPYMRVKETAN"
     gene            complement(19318..>19842)
                     /locus_tag="P5335_03360"
     CDS             complement(19318..>19842)
                     /locus_tag="P5335_03360"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HRZ73950.1"
                     /translation="QKIPESIQFFKHRPDNFPTIRLAQLAQLIHLNKQLFQKCLQTNS
                     VKELYSLFNVNTSYYWKNHYIFDRESPPKNKSLSKNFIDLIILNCIVPIKFCYAMNNG
                     NTNFEELISLVSEIASEKNSIIDKFKKVGINSGNSFQSQALLQLKKEYCNYNKCLNCE
                     IGQKLINFTSLNNN"
     assembly_gap    19843..19942
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20147..21508)
                     /locus_tag="P5335_03365"
     CDS             complement(20147..21508)
                     /locus_tag="P5335_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRZ73951.1"
                     /translation="MKFAENILGTIGNTPLVRINKITKELDCLVLAKVETFNPGNSVK
                     DRMAVKMIEDAEADGRLKPGGTIIEGTSGNTGMGLALGAIVKGYKLICVITDKQSKEK
                     MDILRAVGAKVIVCPTDVEPEDPRSYYSVSKRLGTEIPNSWYVNQYDNPSNAIAHYEQ
                     TAPEIWEQTDGKITHFVVGVGTGGTISGVSKYLKERNPNIKVWGIDTYGSVFKKYHET
                     GIFDENEIYSYITEGIGEDILPKNVDFSLIDGFTKVTDKDAAVYTRRLALEEGIFVGN
                     SAGAAIKGVLQLKEHFKPEDIVVVLFHDSGSRYVGKMFNDDWMRERGFLEEEMTKASD
                     LIKSHIDKPLIIVRTEELVSHAIERLKKYNISQIPVIDSTGFVGSLDETDLFRSYFAD
                     KNIGDKPIREIMGKPYPIVQENTPLEEVTKLINKDNQAVLVALENGKHHIITKHDIIS
                     SIN"
     gene            complement(21593..>22375)
                     /locus_tag="P5335_03370"
     CDS             complement(21593..>22375)
                     /locus_tag="P5335_03370"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73952.1"
                     /translation="TYSKSITEQNLSDLDLNLSYAFDSKNNLSVQYQNLNRIPDHSYT
                     LFQSSYIDYNWYNNFNNEKINSLTAKATTQWVSASLQLSTLNDFLYYSNDDATGEQLL
                     VTPKQYSSTINYLSLNVSKEFKWWKLALDNTLLFQQVDQSENVLNVPQFTTRNTLYFT
                     DYFFKKALFLQTGVTFNYFTNYYANDYNPVISSFYSQSTREIGNFPMFDFFINAKISQ
                     TQIYLKAEHFNAAWTGFDYYSAPNYPYRDFMIRFGLVWNFFL"
BASE COUNT         6934 a         4317 c         3295 g         7629 t
ORIGIN      
        1 tttgagccgt tggatttgtt tgcccaattg agcttaaact aaccaacaaa aagccacaaa
       61 aagcaacata atttttaaac gtaattttac ttgacgtaaa aaacgaacgt gtagagttat
      121 tcataaataa aaattttagt ttagtttttg aagttatgtg gtaaacccct aaaaacgagt
      181 ggcaaatata acataaattt caaaccgttt aacgattgtt attaatttaa aattaagatt
      241 tcattatttt ttttagtcaa ttcattttat tgacagtgtg ctagttagaa tgataaaaaa
      301 aggactacga attcctttta aataacaaca aaaacaacta taaagagact aaaaaagtag
      361 attgtatttt acagctttct ggttttttaa ccataaaaaa agcccctcga ttactcgaag
      421 ggcttttgat gtattcaact tgatttaagc ctgaccggca gaaccaaaat tcaatggaac
      481 ttgtggttgg tctgaatctt taatttcacc atgtgctttt tcaaatcggt gaatgttttc
      541 tcctatggct ttcaacaaac gtttcgcatg ttgaggcgtt agaatgattc tcgattttac
      601 ctttgctttg ggcacgcccg gcattaaggt tacaaaatcc aacacaaatt cagaagcaga
      661 atggttgatg attgccaaat tggaataaat tccttcggct actttttcat ccaattcaat
      721 atttatttga ccttgttgtt gattattatc actcatgatt cttagtaatt atattcttct
      781 ttagctgcca ttatctcgtt gtaatcttct ttcgaaccta caatgttgtt gtcatattct
      841 ctcataccgg ttccggcagg gattctatga cctacaataa cgttttcttt caaaccttct
      901 aagaaatcaa ctttacctgc tacagcagct tcgtttaata ccttagttgt ttcttggaac
      961 gatgccgcag agataaatga tttcgtttgt aacgatgctc ttgtaatccc ttgaagaacc
     1021 ggagtagcgg tagccgtaat cacatcacga gctgacacta gttgtttatc ttcccgtttc
     1081 aataaagaat tttcatcacg caactctctt ggtgtaatca tttgacccgg tttcaagttt
     1141 tgagaatctc cggcatcttc tacgactttc ataccaaaca atttgtcatt ttcgatgatg
     1201 aaatcttttg tatgagccaa ttgttcttct aagaacaacg tatcgcccgg atcttctact
     1261 cttactttac gcatcatttg acgtatcact acctcgaaat gtttgtcgtt gatttttacc
     1321 ccttgcagac ggtaaacctc ttgaatttca tttaccaaat actgttgaac agccgatggt
     1381 ccttgaattc ttaaaatatc ttccggtgta atggctccgt ctgataaaga cattcccgct
     1441 tttacgaagt cattctcttg taccaaaatt tggttagaca atttcactaa gtattttttc
     1501 acttcaccaa attttgattc gatgatgatt tcgcggttac ctcttttaat tttaccaaac
     1561 gatacaaccc catcaatttc agaaactact gctggatttg aaggattacg agcttctaaa
     1621 agttcggtaa ttcttggtaa acctcccgtg atatctcctg ttttagaaga acgtcttggt
     1681 atttttacta aaattttacc agctttaatt ttctcgccat catctaccat taagtgagct
     1741 ccaacaggaa ggttgtacga acgaatcaat tcgccgtctt taccataaat caataaagtt
     1801 gggattaatt ttttatttct tccttctgaa attacttttt cctggaatcc agtttgctca
     1861 tcgatttcaa cttggaatga ttgtccttgt tctaaatctt cgtaagcaat ttttccggta
     1921 aattcagaaa taataacccc gttatatgga tcccatttac aaatggtggt ccctttatca
     1981 acgatttgac catcttttac ataaatggtt gaaccgtaag gaatattgtg agtatttaat
     2041 acaatgcctg tattttcatc cactaatttt aattctgtag aacgagaaat cacgatatca
     2101 attgcttttc catcagcatc ttctccttta accgttttta aatcgtctaa ttctaatcta
     2161 cctttgaaac gagttatgat atttgattct tcagacatac cacccgcaac cccaccaacg
     2221 tggaaagtac gaagtgttaa ctgagttccg ggctcaccga tagactgagc agcaattact
     2281 ccaaccgctt cccctttttg agtcatacga ccggttgcta agtttctacc gtaacatttt
     2341 gcacaaatac cttttgccgc ttcacaagtc aatggtgaac gcacttccac tctttcaatt
     2401 ggagatgctt caatcgctct cattactgat tcggtaattt cttgaccacc ttcaatcaat
     2461 acttctaaat tcaacggatt gataacatct tgaagagcta cacgtcctaa aattctttct
     2521 cctaacgatt caacgatttc ttcattcttt ttcaaggcag aaacttcgat tcctcttaaa
     2581 gttccacaat cttcggtatt cacaattaca tcttgtgata catcgtgtaa tcttctagtt
     2641 aagtaaccgg catcagccgt tttcaaagcc gtatccgcaa gacctttacg agcaccgtgc
     2701 gtagagataa agtattctaa aattgaaaga ccctctttaa agttagaaag aattgggttt
     2761 tcaataattt caccaccacc agcagttgat tttttaggct tagccatcaa accacgcata
     2821 ccggttaact gacgaatttg ttctttagaa cccctagctc cagaatcaag catcatatac
     2881 accgagttaa acccttgttg gtcttctcta atgtttttca ttgctaactc tgtcaataat
     2941 gcatttttag aagtccaaac gtcaattact tgattatatc tttcattgtt tgttattaat
     3001 cccatgttat agttcatggt aatagcctct acttcttctc ttgcatctgc tattaattct
     3061 tgtttttggt gaggaataat aatatcacct aaactgaatg ataatccacc acggaatgca
     3121 aacttgtaac ccatgtcttt catgttatcc aagaaggctg ccgtagttgg cacattggtt
     3181 acagataaaa ttcttccgat aatatcacgc aacgactttt tagttaaaac ctcgttgata
     3241 tatcctgctg cttccggtac tacttcatta aataatactc tacccgcagt agtttgaatt
     3301 aatttatata ctaattttcc ggtttcatca aaatctcttg ctctaatttt aacacgagca
     3361 ttcaattcta atttaccttc atttaaggca atatttactt cttccgcaga atagaaagtt
     3421 aggccttcac ctaaaatttt atgctcagga gttgataaac gttctttggt catataataa
     3481 agaccaagta ccatgtcttg ggatggaacc gtaattggtg caccatttgc cggattcaaa
     3541 atattgtgtg aagccaacat taataattgt gcttccaaaa tagcttccgg tccaagtggc
     3601 aagtgaactg ccatctggtc accatcaaaa tccgcgttaa acgccgtaca cactaatggg
     3661 tgtaattgaa ttgctttccc ttcaattaat tttggttgga atgcttgaat acccaaacgg
     3721 tgtaaggtag gagcacgatt cagtaataca gggtgtcctt tgataacatt ttcaaggata
     3781 tcccatacta caggctcttt tttatctata attttcttag ccgatttaac tgtttttaca
     3841 attcctcttt caatcaattt acggataaca aaaggtttgt aaagttcagc tgccatatct
     3901 ttagggatac cacattcgaa caatttcaat tcaggtccaa cgacaataac ggaacgagca
     3961 gaataatcta cacgtttacc taataagttt tgacggaaac gaccttgttt tccttttaag
     4021 gaatccgaaa gtgattttaa tggtctgttt gattctgttt taaccgctga tgcttttctt
     4081 gtattatcaa ataaagagtc aactgcttcc tgcaacatac gtttttcgtt tcttaagata
     4141 acttccggag ctttaatctc catcaatctt ttcaaacggt tatttcgtat gattacacga
     4201 cggtataaat catttaaatc ggatgttgca aaacgaccac catccaatgg cactaatgga
     4261 cgtaattctg gtggaataac cggaattact tttaaaatca tccattgtgg caagttttct
     4321 cggttaagat ttgcttcacg gaaagattca accacttgaa gacgttttaa cgcttcagtt
     4381 tttctttgtt ttgatgtttc atgattagca gcatgacgta aatcgtaaga caattggtct
     4441 agatttgttc tggctaataa atccataata cattccgcac ccattttggc gatgaattta
     4501 tttggatcat tatcatctaa atattggttt tccattggaa gggtatctaa aatatttaaa
     4561 tactcttctt cggttaaaaa gtctaatctt gataaagaat ctccttctgc attttgggca
     4621 ataccggctt gaattactac atatctttcg tagtaaataa tcatatctaa tttcttagaa
     4681 ggcaaaccta aaatataacc aattttgttt ggaagtgagc ggaaatacca aatatgagcg
     4741 attggcacca ccaaattgat gtgtcccaca cgatcacgtc gtactttctt ctcagttact
     4801 tccactccac aacggtcaca aacgatacct ttgtagcgaa ttcttttata tttaccacaa
     4861 gcacattcgt aatcttttac agggccgaaa attcgttcac agaaaagacc atcacgttcc
     4921 ggtttatggg ttctataatt aatggtttcc ggtttaagca cttcccctct tgactcagcc
     4981 aacattgatt ccggtgaagc caatccgatg gaaattttat taaaccgctt aattgggttt
     5041 ttatctttat tatttctatt attcatcata gtcaataaaa tttgtttatt atgtttcaag
     5101 ttttaagttt caggttgcac taaataggta acctgaaact tgaaacaaca atattattct
     5161 tctaatcgga tatctaaacc aagacctttc aattcatgca ttaatacatt gaatgattca
     5221 ggtaatcccg gttccggcat tggctcaccc ttaacgattg cttcgtaagt tttagctcta
     5281 ccaataacgt catctgattt tacagtcaag atttctctta aagtacttga agcaccataa
     5341 gcctcaagag cccaaacctc catctctcca aaacgctgac ctccaaattg agccttacct
     5401 cctaatggtt gttgcgtgat taatgagtat ggtccgatag aacgagcatg cattttatcg
     5461 tcaatcatgt gtcctaattt caacatatag ataactccaa cggttgctgc ttggtggaaa
     5521 cgttctccgg ttcctccatc atataaatag gtatgaccaa atcttggaat tcctgcttcg
     5581 tctgtcaatt cattaatttg gtcgattgtg gctccatcaa aaattggtgt agcatacttt
     5641 cttcctaaat tctttcctgc ccatcctaaa acggtttcat aaatttgacc aatgttcata
     5701 cgagaaggta caccaagtgg attcaatacg atatcaacag gagttccgtc ttcaaggaaa
     5761 ggcatatctt cgtctcttac gatacgagct acaatacctt tatttccgtg acgacccgcc
     5821 attttatcac ccactttcaa tttacgtttc ttagcgatat aaactttagc taattttaaa
     5881 attccggctg gtaattcatc tccaacagta atggtaaact tctctcttcg taaagcacct
     5941 tgaaggtcat ttaatttgat tttataattg tgaatcaaat catttaccat gaaattagtt
     6001 tcatcatcag aaacccactg accttttgtt aagtgagcga aatcttccac tgcatttaac
     6061 attttttgcg tgtatttttt accttttggt aaaacttctt cgcctaagtc attcattact
     6121 ccttgcgaag tttttccgtt tacgatgttg aataattttt caactaattt ttcttttaat
     6181 tcgttgaatt taacttcgaa ctccatttct aatgaagcca aatcatcttt atcttttgta
     6241 cgtttgcgtt tatctttaac ggctcttgca aataattttt tatcaagaac gacnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacggctt gacgcatcat gttagatccc
     6421 atcaaggcac ggttcgcatc atcatgttct aagaaaggaa ttaaagaagc tgaaatcgaa
     6481 gcaatttgat ttggagcaac gtctgtataa tgaacagcag aaggatccac aaccgggaag
     6541 tcaccttcct cacgggcaat aactttatcc gctaatattt ttccttttcc atccatttca
     6601 atgtttgcct gagcaatcat tttaccttct tcttcttccg cacttaaata aacaggttca
     6661 gaaatcaaat ctaccacccc attcgtaact ttacgataag gcgtttcaat gaatcccatt
     6721 ccatttactt tagcataaac acctagggaa gaaatcaaac caatgtttgg tccctcaggc
     6781 gtttcaattg gacaaagacg accatagtgt gtatagtgaa cgtcacgtac ctcaaatccg
     6841 gctctttctc tcgaaagtcc accaggtcca agggcagata gtcttctttt gtgtgtaatc
     6901 tcacctaatg gatttgtttg atccatgaat tgagaaagtt ggtttgttcc aaagaaagaa
     6961 ttaataaccg aagacaatgt ttttgcatta atcaaatcaa ttggtgtaaa cacctcatta
     7021 tctcttacat tcattctttc gcggattgtt ctggccatac gagccaaacc aacaccaaat
     7081 tgtgcagaaa gttgttctcc aactgttcta acacgacggt ttgataagtg atcaatatca
     7141 tcaatctcag cttttgagtt gattaattca atcaaatatt tcacgatagt gatgatatcc
     7201 tctttggtca acacttgttt ttccattggg atatccaaac ctaatttttt gttcattctg
     7261 tatcgaccta cttcacctaa gttgtaacgt tggtcagaga aaaacaattt atctataata
     7321 ccacgagcag tatcttcatc aggcggttca gcattacgca attgacgata gatgtgttct
     7381 acggcttctt tttcagagtt agttgggtct ttttgtaacg tattatgaat gattgtataa
     7441 tctgcttgat tattatcttc tttatgtaac aaaatagatt tcacattaga atcgataatt
     7501 tcctcaacat tgtctttatc aattatagta tcacgatcta atatgatttc gtttctttcg
     7561 atagaaacta cttcaccggt atcttcatca acgaaatctt catgccaagt gtttaaaaca
     7621 cgagctgcta atttacgtcc gatgtatttt ttaagtcctg ttttagatac tttaatttct
     7681 tccgctaggt cgaatatttc aagaatatct ttatctcttt caaaaccgat ggctctgaat
     7741 aaagtagtta caggtaattt tttctttcta tcgatatacg catacatcac attattgata
     7801 tcggtagcaa attctatcca tgaacctttg aaaggaatta cacgagcaga atataatttg
     7861 gttccattag catggaagga ttgtccaaag aaaacaccgg gtgaacggtg taactgagac
     7921 acaacaactc tttcagcacc attgattaca aaagtaccgc taggggtcat atagggaata
     7981 gtacctaaat agacatcttg tactattgtt tcaaaatctt catgttcagg atctgtacaa
     8041 taaagtttta gtcttgcttt taatggcaca ctataggtta aacctctttc gatacattct
     8101 gaaatagaat aacgaggagg atcgacaaaa tagtctaaaa attcaagtac aaaattgttt
     8161 cttgtatcgg tgattggaaa attttccatg aaggtgttgt atagaccttc gttgcctctt
     8221 tcgtcagatt tagtttccaa ttggaagaaa tccttaaaag attttacctg aatatccaga
     8281 aaatcgggat attgtggtat atttttagtt gaggcaaaat tcaatctttc agtctgatta
     8341 gttatcatct atggacaaaa ttttgattaa aaaaagtaat tatttttgtg taaaacactg
     8401 tttaactaat actttctttt tgcaatcaat acatctttaa aaattttttc agattgctct
     8461 gtcttaattt tctttcttcg tagtgatcaa aaataatagg gtattttaaa ctaattgata
     8521 ataaaataat gtataatttt attatacgaa aaatggttta ggtcgttgga ggttaatcca
     8581 agacctaaac ctagttctta aacctaagtt agcttattta agctctacta cagctccagc
     8641 ttcttctaaa gattttttaa gaccttcagc ctcatcttta gtcacacctt ctttaacatt
     8701 agaaggagca gcatctacta aatcttttgc ttctttaaga cctaaaccag ttaattcttt
     8761 aaccgcttta actacagcca atttagatgc accagcttct ttcaatacta cagtgaattc
     8821 tgtttgctct tctactacag cagcgtctcc gccaccagca gctggaccag caactacagc
     8881 agctgctgca ggctcgatac catattcatc ttttaaaatt gttgctaatt cattaacttc
     8941 tttaactgtt aagttaacca attgttctgc gaattgtttc aaatctgcca ttttttctat
     9001 cgatttaaat agtttgtaaa atataattta ttaagtgcgt tctcgcgggt ttcttatctt
     9061 tcggataagg ttttaacaat tcctgcaatt ttgccaccac ctgatttaag agcggataca
     9121 acatttttag caggagattg tagaagtccg atgatttctc cgataacttc ttcttttgat
     9181 ttaagagata ctaaggcgtc taattggttg tcaccaataa agatagcctg atgaatgtaa
     9241 gctcctttta aaagaggtct atccgatttt ttacggaatt ctttaatgat ttttgcagga
     9301 gcgtttccgt tctctgcaat cattattgaa gtatttcctt ttaatactga aggtaaatct
     9361 ccataatcgt tagcagaagc ttccatcgct ttttccaaca aagtgttttt caccacattt
     9421 aattggatac cggctttgaa acaggctctt ctaagatttg aagttgtttc agcattaaga
     9481 tttgaaatat ctgcaatgta gacaacatta ctatcagcca actgtgcagt taaatcttca
     9541 ataactcttg atttttcttc tctagtcata ctattattat ttaactacca attaaactgc
     9601 tttaggatct aaagcaatag cagggctcat tgtactagac aagtgaatag acttgatata
     9661 agtacctttg gctgctgttg gtttcatttt gattaatgtt tgaataattt cgtgagcatt
     9721 atctacaatc atgtctgcgt tgaaagaaat tcttccaatt ccggcatgaa caatacccgt
     9781 tttatcaact ttaaagtcga ttttaccggc tttcacttct tgaactgctt ttgcaacatc
     9841 catcgtaacc gtacctgttt taggattagg catcaaacct ctaggtccta aaatacgacc
     9901 taatggacct aatttaccca ttacagcagg catcgtgata attacatcaa catctgtcca
     9961 accgtcttta attttttgaa gataatcatc taaacctacg tagtctgcac cagcagcttt
    10021 agcttccgct tctttatctg gagtaactaa tgctaaaact ctaacatctt taccagttcc
    10081 atgaggcaat gttaccacac ctcttaccat ttgatttgct tttcgaggat caactcccaa
    10141 acgaactgca atgtccacag actcatcaaa ttttgcagaa gcaacatctt ttaataatgc
    10201 cgaagcatct tttaaagaat ataatttgct tttttcaatt tttgatgcag cctctttttg
    10261 cttttttgtc aattttgcca tgtctttctt ttaattaaaa aggagcaact cctgatactg
    10321 ttatacccat agaacgtgct gttccagcta tcatactcat agcagcctcg atagtaaatg
    10381 catttaaatc taccattttg tcttcagcga ttgttctaat ttgatcccaa gtaacgctag
    10441 ctactttttt acgatttggt tgacctgaac ctgactttag ctttgttgca tccattaact
    10501 gaatagcagc aggtggagtt tttacaacaa aatcaaatga tttgtctttg tatacagtaa
    10561 tctgtactgg taaaacttta ccgggtttat cttgagttct cgcattgaat tgcttacaga
    10621 actccatgat attaactcca gcagctccca aagcaggtcc aaccggtggc gaaggattcg
    10681 cggcacctcc cttaacttgt agtttaacta ctttactaat ttctttagcc attgtttaaa
    10741 aaaaaattta gcacatctca attggaagca agatgcggtt tataataatg taacaaatta
    10801 tactttttca acttgcataa accctagttc taaaggtgtt tttcttccga aaattttcac
    10861 catcacttca agtttacgct tatcttcatt aattttctca acagttccat tgaaaccgtt
    10921 gaaaggtccg tcaataactt tgatggtttc accaatgttg aacggaatat tttgagaatc
    10981 ggtattaatt gccaactcat ctactttacc taacattctg tttacttcgg ataaacgtaa
    11041 aggaacagga tctccgcctt ttacttctcc taagaaacca ataacacctg taatagattt
    11101 aatgatatgt ggtatttctc cggtcaaatt tgcttcaatc atcacataac ccggaaaata
    11161 aactttatcc tttgctattt ttttaccatc gcgtacttgc actacttttt cagttggcac
    11221 caatacctgg gcaataaaat cgcttaaatg aagtcgatta atttcgttat cgatataagt
    11281 tttaacctta ttttcttggc cacttacagc tctaacgaca taccattttt ttaaactact
    11341 atcagccata acagaaatta tccttttatc aaattaaaaa atcctgatat tgcttttcta
    11401 aaaacttcgt caacacccca tgtagctaaa gcaaagacga ctgtaaaaat cgctactatg
    11461 attgttagac gttgtacttc agaccaaggt aaccaagtta cattagactt caattcttcg
    11521 aaagcatctg ttatgtaatt aacaactttt gtcattctta tttgtttttg cacgggtgga
    11581 gagattcgaa ctcccatcaa cggttttgga gaccgctatt ctacccttga actacacccg
    11641 tttgttaaaa gccagtaata ttcataagaa tagtaactgg ctttatatat ttattgaatt
    11701 aatcaagaat ctcagtaacc tgaccagcac ctacagttct accaccctca cggattgcga
    11761 aacgtaaacc tacattcaaa gcaattgcac ttaataaagt tacttcaata gttaagttat
    11821 cacctggcat taccatttca actccagctg gtaaagaaat agtaccagtt acgtcagtag
    11881 ttctaacata gaactgtgga cgatagttgt tatggaatgg tgtgtgacga ccaccttctt
    11941 cttttttcaa gatataaacc tcagctttaa atttagcatg tggtttaaca gaacctggtt
    12001 tacaaataac catacctctt ttgatatctt ctttagcaac acctcttaac aagataccag
    12061 cattatctcc agcctcacct ctatcaagga tttgacggaa catttcgata ccagtaactg
    12121 tagaagttaa tttctcagca cccataccaa taatttcaac tggatctcct gtattacaaa
    12181 ttcctgtttc gatacgacct gtagctacag ttccacgacc agtaattgtg aatacatctt
    12241 caacaggcat taagaaaggt tttgcaatat ctctttccgg ctcttgaatc caattatcaa
    12301 cagcttccat caattcaaga atttttggag tccaagctgg gtcattattt aatccaccta
    12361 aagctgaacc ttgtataaca ggactattat ctccatcata atcatagaat gataataaat
    12421 ctctgatttc catttcaact aattctaata attccgcatc atcaaccata tccactttat
    12481 tcatgaacac aacgattctt ggaataccta cctgacgtcc taaaaggatg tgctcacgtg
    12541 tttgtggcat aggaccatct gtagcagcaa ctactaagat agcaccgtcc atttgagcag
    12601 cacctgtaac catgtttttt acgtaatccg cgtgaccagg acagtcaacg tgtgcgtaat
    12661 gacgattagc agtttcatac tcaacgtgag atgtattgat agtaatacct ctttcttttt
    12721 cttcaggagc attatcaatt tgatcaaatg attttgcttg acagtaacct gcgtcagata
    12781 atactttagt aatagctgca gttaatgtag tttttccatg atccacatgc ccaatagttc
    12841 caatattaag gtgtggcttg ttacgattaa aattttcttt tgccatttta attaatttaa
    12901 atcttagtta tataatagtg ttaaatctta caatttagag ccaatgacgg gaattgaacc
    12961 cgtgacctct tccttaccaa ggaagcactc tacccctgag ctacaccggc ggtaaagttt
    13021 ttttgtttca cgtttaaagt ttcacgtttt ttaaaaacac ttgaaacttg aaactttcaa
    13081 actttttttg tggggagagc aggattcgaa cctgcgaagg tttcccaacg gatttacagt
    13141 ccgtcctcgt tggccgcttg agtatctccc ctgaccttat aacatcaaaa aaaacaatga
    13201 acttaactat tttagaatct tattcttaca aaataatttt attgtttaat tcttattgag
    13261 ccgatagagg gactcgaacc cacgacctgc tgattacaaa tcagctgctc tagccagctg
    13321 agctacatcg gcagtactca ataaaaaaag tccgctattt ctaacggact gcaaatgtat
    13381 agattttatt tgtcaatcaa aaacattttt taaaaaaata tttttagatg tgtgttctaa
    13441 ttttttcttt tctttttagc aacaatcttt ctaaagcttc gctagaagaa tcaactgctt
    13501 cttcaaaagt tttacattgt tttttaacta ttaaatcatc tcccggtaca tgaactttaa
    13561 tctccactat tttattttcc ttttcactag tcgcttcaac tttcagaaaa acgtcaacca
    13621 caacaacttt gtcgtaatat ttttccaatt tatccatgcg ttctgtcaca aaatctacta
    13681 actttctgtc aacattaaag ttaacggcgt taacatttac cttcataatc aaatttttaa
    13741 agtttaacaa taagaattac ttttgatttc tgggatgtgc ttctgaatat actttcttaa
    13801 gttctgacaa actgctgtgt gtatatactt gtgtagaagc caaactagaa tgtcctaaca
    13861 attctttcac tgaatttaaa tcggctccgt tattcaaaag atgcgtagca aacgtgtgac
    13921 gcaaaatgtg agggcttttc tttaccttct cggagatagt actaaagtac gtattaatta
    13981 atcgatatac aagagagtca tttaatttaa cacctttttg tgtcaaaaac aattgctctg
    14041 tatttttaat atcctgaata gcattgcgtt gtataatata catttttatc tgactaatta
    14101 taataggtaa taaaggcaaa attcgttctt tattcctttt tccgatgacc ttcagtgtat
    14161 tactattaat atcaatcgaa ctcagtttta aattaattaa ttctgctctt ctgatacccg
    14221 ttgtatagaa catatctata atgagtttat ctcgaatccc ttcaaaacca tccggataaa
    14281 taatttgatt caacactaag tctacttctt tttcagaaaa aggaatctgt atttttttgg
    14341 gtgttttaag ggctttatgc ttcaacaaag gactttgttc aatttgcttt attcttaata
    14401 agaatttgta ataacttttt aatgaagaaa ttttacggtt tactgatgat gcagaaatat
    14461 ttccatctac taatgagaca atccaacttc gaatttgaga ataacctact atatcaattg
    14521 aatcttgttc aaaatgaatt ttattataag aatcaaactc ttctatatct tttacataag
    14581 ctttaacggt atgcaaggaa tagttttttt gcatttgtaa ataatcctta aaagcttgtt
    14641 gatttgattg cataaaaaaa ccgttagcct caaagttaca aaacttctca aactaacagt
    14701 ttatttttat aaaaaaagca attacatttc tacactgtct cgcaatgttt gaatgtactg
    14761 agctttttga acttgtgctc ttttaacaac agatggttta ataaaagcct gacgtgctct
    14821 aagttgacga acagttccag ttttatcaaa ttttcttttg tagcgtttta acgctctgtc
    14881 gatattttct ccgtctttaa ttggtataat taacataatc tagacacctc ctctcattta
    14941 gggtgcaaaa gtaacattta ttttgaattg tgaattatga aatttgaatt attttacagc
    15001 aggctgataa ttctgcttgt caataatcat ttttgacaat atctctttta agatttctga
    15061 agttccaccg cctataggtc ctaaacgact atctcttgat aaacgtgcca agggatactc
    15121 ttccatatat ccgtaaccgc ctaacatttg taggcaacta tagattgttt catcagctac
    15181 tttggtcgat tttaatttag ccatagtcgc ttctttaact acatattctc ctttgtttaa
    15241 tcgagcaaca gcagcgtaat taaaaacttt gcagtgttct acatcagtag catgctcaac
    15301 catagtatgt cttaatgctt gaaatttatt aatggtacta ccgaatgctt cgcgttgtga
    15361 catgtattct atcgtatagt caattgcata ctctgaacgg gcatgtgcgt tgatagccat
    15421 gattaaacgt tctaaagcaa agtgttgcat aatataagga aatccttttc cttcttcacc
    15481 cattaaattc ttttcagaaa tttcaacatt gtcaaatgca atctcagctg tatccgaagc
    15541 cttccaacct aatttatcaa gttttgtagc ggaaattccc gggttagaag tatcaactaa
    15601 aaaaatactt attcctttat ttcctaattc cgggcttgtt ttagcggcaa ctacataata
    15661 atcggcatac accccgtttg taatgaaagt cttggaacca tttaagtaat atttatcacc
    15721 ttttttaatg gctgaagttc gcattccggc cacatcactc ccaccaaaag gttctgtaat
    15781 acacaaagct ccaatcttat ctccggaaat acttggcact aaataatctt gttttatttg
    15841 ctcatcacct tctgcattaa ggtgcgtcat ggctaaataa gcatgtgccc acatggccgc
    15901 agcaaaaccg gatgatttta ttttttgcaa ttcttccaaa aagataacag tgtaaaacaa
    15961 atctaaattc attccgccgt aggcttccgg ataatttatt ccaaaaaaac ccatctcacc
    16021 aaacttcttc cagataaaac gttcaatagt tcctgttttt tcccattttt caatgtgagg
    16081 cacaacttct ttatgtaaaa aatcgcgtaa tgttacccta aatagttggt gctcttcagt
    16141 gaaataaata gaattcataa ataaataaaa gttgtttttt ttctatattt ttttagaatt
    16201 ccaaatataa ggcgataatt ttgatttttt cgttaggcat tcctttaatt tttgttaaat
    16261 cctcaaaaga atgaatacca ttattcatgc ttctgtaagt aacaatttct ttagcaatgg
    16321 tatagttaaa ataaggaaac ttcgctaatt cttttacaga caaattatta acatcaattt
    16381 tatttactgt tggaattgtc gtaacctgaa aatatttatt caaattttca attacttccg
    16441 gacttaatcc ccaaatggat tccatttgtt ccatcgttac aaaaccaccc agaatatctt
    16501 tttgctttaa aattctttca gacaaagcag gtccgattcc ataaatcttt atcaaatcat
    16561 cttgtgttgc ctcattgatg tcttttcgaa caatactttt tttattctcg taggtatttt
    16621 tagcgaaaga tgaaaatgct tttttaggtt gatttaccca atctggaaat ttaaaataag
    16681 gcgaaatcgc taatagcaaa ctatctgaaa cacctgtaac ttgttgaaat tcctgaaccg
    16741 aattcacata tttgttttgc tttctaaatt caaatagtct atttatttct tctaaagaca
    16801 tccctaactg atacccttta tagtctgaaa taaaatttgg attaaaagga tatatctttc
    16861 tttcagtgga agattgactt atctttaaac tatctataac cgattgcatg gctaaccaac
    16921 ttttatcgga agttgatggt ttcgttgttt tgcttgaaga ccaataaaaa taactcaatt
    16981 gaataataac tataagaagt aataaagaaa gcacacctct tcgttgttct tttgtaaact
    17041 ggaagtagga tttaatcttc ataagaaaat gtttaaaaat tattcgaatt ttttaatctc
    17101 tccggattta agctttctga aatattcttt taaatcagct ttaacctcac ttgaaagaaa
    17161 atataacccc agaatatttg gaaaagccat acctaaaatc atcatgtcag aaaaatcaat
    17221 tacagcacct aaactggcgg aagacccaac tacaacaaaa accaaaaata atattttgta
    17281 tacattttcc attttttttg tgtttccgaa aagataagtc catgatttca ttccgtaata
    17341 agaccaagaa atcatagtag agaaggcaaa taatataata gctacgagca atacatattt
    17401 aaaccatggc aacacactgc taaaagccgc tgaagttaat gcagaacctg ttaatccttg
    17461 tgtgtcttga gcatatcccg aaaatatgag caccaaagcg gtcatggtac aaactacaat
    17521 ggtatccaca aacggctcta ataaagaaac gattccttca ctcaccggct catctgtttt
    17581 tacagcggaa tgtgcaattg cggcggaacc gacacctgct tcatttgaaa aagcagctcg
    17641 ttgaaaaccg acaactaaaa caccaattac acctcccgat attccgtcag ctgtaaaagc
    17701 tccggaaaaa atttcggaaa aagcatctcc aatatgagca aaattgacaa atatgattat
    17761 taaagaagta attacatata atcctaccat aaaaggcacg attttgtctg ttacgcttgc
    17821 aatcttttta attcctccta taataacgat tccaacaaaa aaggcaacaa ccaaaccata
    17881 ccaaaaacca tttcctataa aaacaggaaa cacattagcc aattgggcaa aagattgatt
    17941 ggcttgaaac atatttccgc ctccaaaaga acctccaatg gctaaaatgg aaaaaacaac
    18001 agctaatacc ttaccaaatc cggcatatcc ctttcttttt aaaccttcag aaagataata
    18061 cattggaccg cctgaaactt cccctaagtc gtttaatcgt ctatacttta cacctaaagt
    18121 acattcgaca aatttagaag acatgcccaa aaatccggct aaaatcatcc aaaatgttgc
    18181 tcccgctcct ccaacagaaa tggcaacagc aacaccagca atatttccta aaccaactgt
    18241 tcctgaaaga gctgttacta atgcttgaaa gtgagaaacc tctcctgcat ctcctggttt
    18301 atcgtaatct ccacgaatta actgataagc gtgcttaatc ccttttaaat tgataaaccc
    18361 catataaatg gtaaaaaaag tagctccaac aacaagccat agtacaacca agggcatatc
    18421 aaatccgaag gaagcgaaag gtttatagaa aaaaacactc gccatagcat ctacagccgg
    18481 ttttatcaca tcattaattt tttcatctaa tcctttttcc tgactaaaag ttaaaatagg
    18541 tagtaaaaga gcaaaaagcg agaataactt cttcataaag ttaatttaag tttaatttag
    18601 ttcagcaaga tgcgaaaaaa attgctttaa aaaaaaagta taagggattt tttaaaaaaa
    18661 ctcaattata aatcaaagac tgaactgcgt ttagtgtgta acatatcttt taaccttaac
    18721 caaaatgcca aggtcaaata aacgccaaac caaagaccaa ctgtgacaaa agatatatag
    18781 ataaaaaaca gacgaacgtt tgaagctctc atgcctaaac gatcggctaa cctggaagaa
    18841 acatgaaaac catgcttttc aaaaaaatga cgaattgaag taatccagct cattaattag
    18901 cagtttcttt cacacgcata taaggtgttg tttcttcttc aacatttgtg acaaaataag
    18961 gagaatctcc ccaccaaaaa aaagttcgat atctttcctc gtatttcaat ttaacatact
    19021 tgccttgaag atcttctaat tgttgaatca cttctttttc tttgttagcc actgaaaaac
    19081 taaaaatttg tgctcctgaa attccctgac tcatttcccc ttcccaagtt ttaaaaacaa
    19141 cccctttttt actgaatttt ataagctcac cggaacgtac tccttcacta tagggcacat
    19201 acattataaa caaaaagtac ccaacaaaag aaagcaaaaa cagaaataaa aatcgataaa
    19261 aatatttttt aaccttttgt gaaaaagaag gtttagaaga gttatttgtt tccattttta
    19321 attgttattt aatgatgtaa agttaattaa cttttgtcca atttcacaat ttaaacattt
    19381 attataatta caatattctt tcttcaattg caatagtgcc tgagattgaa aactatttcc
    19441 tgaattaatg ccaacctttt tgaacttatc aatgattgaa tttttttctg aagctatttc
    19501 tgaaactaaa ctaattaatt cttcaaaatt tgtatttcca ttattcattg cataacaaaa
    19561 cttaattgga acaatacaat tcaaaataat taaatcaatg aaatttttgg ataaactttt
    19621 atttttagga ggactttctc tatcaaatat ataatgattt ttccagtagt aggaagtatt
    19681 gacattaaaa agagagtaca attctttaac cgaatttgtt tgaagacatt tttgaaacag
    19741 ttgtttattt aaatgtatta attgagctaa ttgagccaat cgaatagttg gaaaattatc
    19801 cggacgatgt ttaaaaaact gaatggattc gggtattttt tgnnnnnnnn nnnnnnnnnn
    19861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19921 nnnnnnnnnn nnnnnnnnnn nngtatttac tattgatatt tgcttaccat caacagaata
    19981 aagatttgta aaattgaatt ttttgtattg ccatacaaaa tgcagaaagt cttctttcat
    20041 ggcgattaat gattttagta ttgtggcgat taaatgaaat actaagatat aaaaaaattc
    20101 cttaccaaaa aaattgataa ggaattataa aaaatcgaat acctttttag tttattgaac
    20161 taatgatatc gtgtttagta atgatatggt gttttccatt ttctaaagct accaaaacag
    20221 cctgattatc tttattaatc agttttgtaa cttcttccaa cggggtgttt tcctgaacaa
    20281 ttgggtaggg tttccccata atttcacgga taggtttatc accaatattt ttatcagcaa
    20341 aataacttct gaataaatcg gtttcgtcta gcgagcccac aaaaccggtt gaatcaatta
    20401 ctggaatttg agaaatatta tattttttta atcgttcaat ggcgtgtgaa accaattcct
    20461 ctgtgcgaac aattataaga ggcttgtcta tatgcgattt aattaaatca gaagctttgg
    20521 tcatttcttc ctctaaaaat ccacgttcac gcatccaatc atcattgaac attttaccaa
    20581 cataacgact tcctgagtca tggaataaaa cgaccactat gtcttccggt ttaaaatgtt
    20641 ctttcaactg caacactcct ttgatagccg ctccggctga attacctaca aaaatgcctt
    20701 cttcaagtgc taatcgtcta gtgtaaaccg cggcatcttt atcggtaact tttgtaaagc
    20761 catctattaa agaaaaatca acatttttag gaagaatatc ttctccaatt ccttcggtaa
    20821 tgtatgaata gatttcattt tcgtcgaaga ttccggtttc gtggtacttt ttaaatactg
    20881 aaccgtaggt atcaattccc caaactttaa tattaggatt tctttctttc aaatatttcg
    20941 aaacacctga aattgttccg ccagttccaa ctcccactac aaaatgagta attttcccat
    21001 ccgtttgctc ccaaatttca ggagctgttt gttcgtaatg tgcaattgca tttgatggat
    21061 tatcgtattg attaacgtac caagaatttg gaatttcagt acctaaccgt ttagaaacag
    21121 aataataaga tcttggatct tccggttcaa catcggttgg acatacaatt acttttgctc
    21181 caaccgctct taaaatatcc attttttctt tggattgttt atcggtaatc acacaaatta
    21241 atttgtatcc tttaacaatc gctcctaatg ctaaacccat tcctgtatta ccggatgttc
    21301 cttcaatgat agtaccaccg ggtttcaaac ggccatcagc ttcggcatct tcaatcatct
    21361 taacggccat tctgtctttg actgaattac ccggattaaa agtttccact tttgccaata
    21421 ccaaacaatc tagttctttg gtgattttat taattctaac taacggagta tttccgatag
    21481 ttcctaagat attttctgcg aatttcatgc tatttatttt tcgattgtaa aggtattgat
    21541 aaaaaaagat actgcaagtt ttgttggtaa tttcaaaaaa gttgggtttt aattataaga
    21601 aaaagttcca tactaatccg aaacgaatca taaaatctct ataaggataa tttggagctg
    21661 aataataatc aaaacctgtc caagcagcgt tgaaatgttc cgctttaagg tagatttgag
    21721 tctgactgat tttagcattt ataaaaaaat caaacatcgg aaaattacca atttctctgg
    21781 tagattgact gtagaaacta ctaattaccg gattataatc attggcatag taatttgtaa
    21841 aataattaaa tgtcacaccg gtttgaagaa acaaggcctt tttaaaaaaa taatccgtaa
    21901 aatataatgt attacgtgta gtaaattgag gaacattcaa gacgttctca ctttgatcta
    21961 cttgttgaaa taagagggta ttgtcaaggg caagtttcca ccatttaaat tctttgctaa
    22021 cgtttaaact caaataatta attgtgcttg aatattgttt aggggtgact aataattgtt
    22081 cgccagtagc atcatcatta ctatagtata aaaaatcatt caaagttgaa agttgcaagg
    22141 aagcagaaac ccattgggtt gtagcttttg ctgtaagtga attaattttt tcattattaa
    22201 aattattata ccaattgtaa tctatataac tactttggaa taacgtataa ctatgatccg
    22261 gaatacggtt taaattttga tattgaacac ttaaattatt ttttgaatca aaagcataac
    22321 tcaaattcaa atctaaatcg gataaatttt gttcagtaat tgatttagaa taggt
//
LOCUS       DAOTCW010000023        22065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1252_length_22065_cov_37.724716, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000023 DAOTCW010000000
VERSION     DAOTCW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 22065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22065
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1252_length_22065_cov_37.724716"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="P5335_03375"
     CDS             complement(<1..877)
                     /locus_tag="P5335_03375"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRZ73953.1"
                     /translation="MNNIIPTVELETDDELFEHHRFEVPKGQSMLRIDKYLMQVVENA
                     TRNKIQQAATAGNILVNEQPVKSNYKVKPLDVIRVLMPHPPFEYLLEPENIPINVIYE
                     DDQLLVVNKEAGMVVHPGHGNYSGTLVNALAYHFENLPMNSSERPGLVHRIDKNTSGL
                     LVIAKTDLAMAYLTKQFAAKTSEREYIALVWGNVEEEEGTVRGYIGRHVKDRMQMAIF
                     EDESMGKPAVTHYKVIERLGYVTLISCKLETGRTHQIRAHMKHIGHTLFNDERYGGHN
                     ILKGTTFTKYKQFVDN"
     gene            complement(870..1484)
                     /locus_tag="P5335_03380"
     CDS             complement(870..1484)
                     /locus_tag="P5335_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRZ73954.1"
                     /translation="MSLRNYLTSKVFFSQVLIAFGIIAVLAFVFFNMLSFFTNHGQEI
                     TVPDLSKMNVEQAEETLDELGLDYVLLDTLDYNKDFPQFSVVEQEPKKGAKVKANRKI
                     YIKVNASGYTLVVLPDLIEKTFRQAEPTLKSIGLQVGEVTYKPYLGKDMVLEMRYKGA
                     PVKPGTKIMKTSKIDLVLGDGKVAFDETEIDSLIREQESNPTNE"
     gene            1589..2563
                     /locus_tag="P5335_03385"
     CDS             1589..2563
                     /locus_tag="P5335_03385"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRZ73955.1"
                     /translation="MKNVAIIMGGYSSEYQISLTSGNVVYQYLDKSKYNPYRIHIFKE
                     KWVLVNDDKNEFPVNKNDFSVSMNGEKLNFDVVFNAIHGTPGEDGLMQAYFELLTIPQ
                     TSCDYYQSALTFNKRDLLSVLKPYGIKTATSYYLNFGDAVEPKAIIKTVGLPCFVKPN
                     KSGSSFGISKVKTKEELIPAIENAYKEDNEIIIESFLDGIEVSVGVINYKGKITVLPI
                     TEIVSENDFFDYEAKYLGKSQEITPARISEELKEKIETVAKRAYEVLKMKGFSRSEFI
                     IVNNEPYMLEMNTIPGMTTESILPQQAKVAGISLSDLFDNAIELALTK"
     gene            2571..3026
                     /gene="coaD"
                     /locus_tag="P5335_03390"
     CDS             2571..3026
                     /gene="coaD"
                     /locus_tag="P5335_03390"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRZ73956.1"
                     /translation="MKKAIFPGSFDPITLGHFDIIQRGISLFDEVIVAIGVNAEKKYM
                     FSIEQRMKFIEEAFKDEPKVRVITYEGLTIDLCKKENAEFILRGLRNPADFEFEKAIA
                     HTNRRLSKIETVFLLTASKTSYISSSIVRDVIRNGGDYTVLVPDSVKIK"
     gene            complement(3075..3935)
                     /locus_tag="P5335_03395"
     CDS             complement(3075..3935)
                     /locus_tag="P5335_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ73957.1"
                     /translation="MKKITLLFLLLFGFIGISQTISHSTSMTLSNTNVACNGGTPSTS
                     SDNRYFRYFKLSDFSITADYTISSVQFGVQTLTIPTLPDGFPVTVKIYATTSNNFPVA
                     YPAGYTELAQVTTNFLLADVETLVSVPIAATIPAGSNVLVEVGYLAQTASSGNRIFLS
                     ANDLGESAPTYISSTACALVPPTPLASINFPNAHLVLSVTGTTLGVKESILQKVSVFP
                     NPSKGKFTVDLPASVSIEKSTLTDITGKQMAINLDASNSFSISEYNTGIYILNLQTSE
                     GVLNKRLIKE"
     gene            4028..>5174
                     /locus_tag="P5335_03400"
     CDS             4028..>5174
                     /locus_tag="P5335_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRZ73958.1"
                     /translation="MLKYLHFSLFLICFLSFSQNKTSILTPFEKGNGNQSTTYEECIS
                     FYKNLATNYSTIQILEKGKTDSAKSLHIVVFSENNNFDFDQDKAIVLVNNGIHPGEPD
                     GIDASMMLLRDLATSKIKVPKNTLVVVIPVYNIDGMLNRNSSSRANQNGPEAYGFRGN
                     GRNYDLNRDFIKSDTKNSRSFQELFRSINPDVFLDNHVSNGADYQYTFTCIATQHERL
                     GTTLGNYFTSEFFPDIVKSMNQKKIDVVPYVNVHSTTPDQGYEQFTDTPRYATGYSSL
                     FNTLGFVPETHMLKSFKERVKVTYEFMVSVINYTDANWNKIKKIRTKSYNDYQQGTNY
                     PIDWAIDSTKVSYIDFKGFEGLKKPSDISRKDRLYYDRSKPFTKKIPF"
     assembly_gap    5175..5274
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5275..5742
                     /locus_tag="P5335_03405"
     CDS             <5275..5742
                     /locus_tag="P5335_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73959.1"
                     /translation="LIQKDTLIEVETYKIDSYQTSKSPYEGHYVHYNTKISKSNKKVK
                     FNSGDYIFFSNQTGVKYLLETLEPEAIDSYFNWNFFDAILQQKEWYSAYVFEDLAMDI
                     LKQNPILKENFEKKKMEEEKFAESGSAQLDWIYRNSIYFESSYLTYPVYRVLK"
     gene            complement(5749..6333)
                     /locus_tag="P5335_03410"
     CDS             complement(5749..6333)
                     /locus_tag="P5335_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRZ73960.1"
                     /translation="MYKVFVNDKPLFLTNEIQKETDFQLFLLESVDIKELIIKIFKNK
                     VDKAFLYHPDEKEMMKKLKSKIPVEKAGGGLVYNDKNEVLFIFRNKKWDLPKGGIEKG
                     EEIEDTAIREVEEETGVKGLKIIRKLPKTYHIFKRNGRYKLKITHWFEMRTSFNEKLI
                     GQVEEGIEKVEWKTPKEIQVALTNSYENIKLLFE"
     gene            6341..6988
                     /gene="pyrE"
                     /locus_tag="P5335_03415"
     CDS             6341..6988
                     /gene="pyrE"
                     /locus_tag="P5335_03415"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRZ73961.1"
                     /translation="MILNKDTAKKTAELLLQINAIKLNPKNPFTWASGWKSPIYCDNR
                     ITLSFPPIRNYLREEFSKHIEKQFGKPDVIAGVATGAIGIGMLVAEYLGLPFVYVRPE
                     PKKHGRQNQIEGFLQKGQNVVVVEDLISTGNSSLLAVEALKEAGANVKGMVAIFTYGF
                     DVSVDNFKKANVDLYTLSNYDNLLELAVAKNYITEEEQHTLQEWRVSPSTWGVEV"
     assembly_gap    7159..7258
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7259..7354
                     /locus_tag="P5335_03420"
     CDS             <7259..7354
                     /locus_tag="P5335_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRZ73962.1"
                     /translation="FDGDFNPMMAMMIKGPISKFIETLAANMHKL"
     gene            complement(7357..10353)
                     /locus_tag="P5335_03425"
     CDS             complement(7357..10353)
                     /locus_tag="P5335_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRZ73963.1"
                     /translation="MLKFLRIFFLLFLLISVVNCATKKAANSNAVISESTSKPTTDKN
                     IEDFENLTFNYSLSSPEEKKWVDSIFSSLSTEEKIGQLFMVAAYSNKDSTHIKSLDKL
                     IEEQKIGGLIFFQGGPVRQAQLTNRFQKKSKVPLFIGIDAEWGLSMRLDSVNRFPWNM
                     TLGAIQDLNLIEKTGELMAIQSKQLGVHFNFSPVLDINTNPKNPIIGNRSFGEDKKNV
                     AERALALMIGLQSNGVFATGKHFPGHGATESDSHHMLPVVNFSQEHIRDIELYPYKKL
                     INKGLASVMVAHLDVPSFENRPGYPSSISNVIVTDLLKKELNFKGLIFTDALNMKGAS
                     NFKKPGDIDLAAFTAGNDILLFPENVPVAIQKIKEAIDSALITQDRLSYSVKKILHFK
                     YKAGLNANKPIEIQKLSSAINTPQDEGLQYHLYENAITVIKNKGAIIPIKELDKDKIA
                     YVKLGDDSNTVFLETLKKYTDITEVKHENIDSLNVLLRDYTKVILGFHKADGAWKKHD
                     FSAKEIVWITEIAKRKKVILDCFVKPYALSSIPTFENIDGVIVSYQNSDVAQNVSAQL
                     IFGAIEAKGKLPVTINEEFKVNHGLSTEKLNRLGFTPPQNVKMNPLVLQRIDAIMQKA
                     IDEKMTPGGQILVARKGKVIYQKAFGFHTYEKQMQVKNSDIYDVASLTKIVSTLPNVM
                     QLYDNKKVTLETQLGEMLPLFIGTDKENIIFKDLLSHYAGLQAWIPFYKQTLDSNNLP
                     SEKYYRKAYSTEFPNQVADNLYLRKDYHDTIMKAIAESKLLPKKEYKYSDFTFIILKN
                     YLEKTTGKTLDELSSKNFYSKLGMNNTLYNPLHKFDMSKIPPTEIDTYFRYQTIQGYV
                     HDMEAAMEGGVGGHAGIFSNSMDLAKMMQLFLQKGSYGGEVYFSANTFEAFNTCYYCE
                     NGNRRGIGFDKPQLGTSGPTCGCASMTSFGHTGFTGTMAWADPEEEIIYIFLSNRTFP
                     NAEKNILSQKNIRESIQQVIYDAIIK"
     gene            complement(10364..>10516)
                     /locus_tag="P5335_03430"
     CDS             complement(10364..>10516)
                     /locus_tag="P5335_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HRZ73964.1"
                     /translation="HKSLLDFKRMAKEKAVSENTIVFNNLVNTIEEWKENWEVPASDS
                     WHSRFF"
     assembly_gap    10517..10616
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10617..11001)
                     /locus_tag="P5335_03435"
     CDS             complement(<10617..11001)
                     /locus_tag="P5335_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HRZ73965.1"
                     /translation="MYIPFDSLPEESRIWIYQSNRKFSDDELTEIEIDLQSFIDEWES
                     HGTSLEASFITKYNRFLILAVNQEVQQATGCSIDKSVEFIQKLEQKYKVDLLDKMNVT
                     YKQGEFLTHKSLLDFKRMAKEKAVSE"
     gene            complement(11082..11873)
                     /locus_tag="P5335_03440"
     CDS             complement(11082..11873)
                     /locus_tag="P5335_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRZ73966.1"
                     /translation="MAENLVVPTMAEMMAQGKQPEVLFWVGCAGSFDDRAKKITKAFV
                     RILNRANVSFAVLGTEESCTGDPAKRAGNEFLFQMQAMMNIQVMNAYEVKKIVTACPH
                     CFNTIKNEYPELGGQYEVMHHTQFLKSLLEDGRLTIEGGQFKGKRITFHDPCYLGRAN
                     NVYEAPRELIQKLEVELVEMKRSKANGLCCGAGGAQMFKDAEPGKKEINIERTEDALE
                     TKPDIIAAGCPFCNTMMTDGIKNKEKETSVKVMDVAELIANAQDL"
     gene            complement(11910..12356)
                     /locus_tag="P5335_03445"
     CDS             complement(11910..12356)
                     /locus_tag="P5335_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HRZ73967.1"
                     /translation="MKTEDINKKLQAITENGQHLSPILPEGIKNYLIDIDGTVCDDIP
                     NEEPERMLTAEVYPDALVTLNKWYDEGHIIFFFTSRTEAHREYTEIWLKKHGFKYHGI
                     LFGKPRGGNYHWIDNHLVKATRYRGKFTDLVEKEVTIQVFDDEHHE"
     gene            complement(12439..13764)
                     /locus_tag="P5335_03450"
     CDS             complement(12439..13764)
                     /locus_tag="P5335_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRZ73968.1"
                     /translation="MSYLDNILFLIILSLGFGYFALNVKKIVRNIKLGQQVNRTDNSS
                     ERWKNMTMIALGQSKMVKRPIAGFLHIIVYVGFIIINLELLEIVIDGLFGTHRVFSFM
                     GGFYNVLIASFEILAVLVLVSVIVFWLRRNVIKLKRFVSSDLKNWPKSDANYILYFEI
                     ILMSLFLIMNATDLWLQEMNVHHYNQAGSFPISQFISPLFDGLSEGVVVVIERTAWWL
                     HICGILVFLNYLYFSKHLHILLAFPNTYFADLNAKGKFDNLESVTTEVKLMMDPSADP
                     FAAPANPETVPAKFGASDVQDLNWVQLLNAYTCTECGRCTSSCPANLTGKKLSPRKIM
                     MDTRDRLEEVGKNIDLNNGVFLSDNKSLLNDYITTEELWACTSCNACVEECPVNISPL
                     SIIMDMRRYLVMEQSAAPMPINAMMANIENNGAPWQYNQMDRLNWKEEN"
     gene            complement(13771..>14360)
                     /locus_tag="P5335_03455"
     CDS             complement(13771..>14360)
                     /locus_tag="P5335_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HRZ73969.1"
                     /translation="KGDIKSGLTALVSEKLQPLQEKIEKVMVSTDILLSNLNETLDAN
                     TKQNIKNSISKFSETMAEFSDISKSVNIMLTDNKSKINNSLANFEKTSGNFAKISDSL
                     AQVNIKQTVQNLEKTLANIDKIMADLENGNGTMGKLLKDDKMYSNFTKASKELELLLE
                     DLRLNPTRYVNVSLFGKKNKPYVAPTEIDSTKIKN"
     assembly_gap    14361..14460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14461..14897)
                     /locus_tag="P5335_03460"
     CDS             complement(<14461..14897)
                     /locus_tag="P5335_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRZ73970.1"
                     /translation="MKLTKEIKTAILVISSILLFIWGYSYLKGTDILTDYNTFYIEYN
                     EVEGLVNSSPVTINGLVIGKVKNISFINMSSWKPMVEIQINKEYSIPKSSIALIYEPG
                     LIGGKMIQIVPGIENPSVLAESGDYLKGDIKSGLTALVSEKLQP"
     gene            complement(14937..16115)
                     /locus_tag="P5335_03465"
     CDS             complement(14937..16115)
                     /locus_tag="P5335_03465"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRZ73971.1"
                     /translation="MYFLNKFKFIFFAVLLLSTVNLFSQTNTKFKVVLDPGHGGKDFG
                     ANYYGFIEKNIALKVALKVGRNLEKHNDIQVIYTRNTDVFIELMERARIANRANANIF
                     LSIHCNASPKGDAYGTETFVMGLTKNASNLEVAKKENAVITLESDYKLKYAGYNPNSP
                     ESVIGITLLQEEYLDQSITLSTKIQNEFTNKLDRKNRGVKQGPFLVLNQTSMPSILTE
                     LGFLSYKPEGEYLNSDKGQEELAKGISDAILNYKKEYFGGTNVIIDEKPSKVTDEKVE
                     KDTPVVQNKSNTKEVEKQKEVITNEPIPDSKGITFKIQISASGKKLDLVPQNFNGLSP
                     ISVIDEKGLYKYYFSQTSNYDEAKKYLSEAKNKGYESSFIVAFKNGNKISIQEALKAN
                     "
     gene            16198..18918
                     /locus_tag="P5335_03470"
     CDS             16198..18918
                     /locus_tag="P5335_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRZ73972.1"
                     /translation="MALQKSSHNFTKIALKHLRTNLFHIVLSAFFLTIGFPKVSAQEL
                     QNKTLKVEAQKPVDSLIINNEDFFQIKDTVINDSLQPNKKKPLLEDKIKYKADKYTKI
                     DQKQKLITLYDKAELYYQDIELKAGIIIMNYEKNEVYAGRIKDSSGVYTQNPVFKQGS
                     NVVEPDSIRFNFKTQKALIWNSRTEQGEFRVKAEISKKENDSVYFLRGARFTTSKDID
                     NPEYYFYTNKIKLVPRKKVVTGFTNMVIANVPTPIAIPFAFFPMTEESSSGLIIPTFQ
                     DTRSQGYSLQNGGYYLALSDYYDLAILGDYYTNGSWGLRFQSSYALKYRFNGSFNLRF
                     ENIIQSERGFPDYSKSKNYNIQWSHTQDAKSNPNSRFSASVNLGSSKYFQQSLNQINV
                     GSNLVNNLSSSVSYSKTFNTVPQVNMSLSATHNQNTGTEEINMTLPTLQASVDRIFPF
                     ASKEGAKKGLIKNINLQYNLRGEYRINTYDSLFFKPEMFKEAKTGMQHTIPLSTNFKI
                     FKHFSVSANANYNEVWTLKTINKAFDEELNKVVTNEVSGFDSYRTYSFGSNLGTTIYG
                     TFNFGEAKKIQAIRHVMRPSVSYSYTPSFEKYFDTYAIDASGKMDDYTRFEGGIFGEP
                     SNTYSNFAGLSLGNTFEAKVTEKDSTKTEPKKVMLLNQLNFSTSYNLASDSLQWAPVR
                     MSGGTLLFNNKMNVNFAATLDPYAINAAGRRIDKFNIDNGGSLFRMTSANMTANYSFS
                     SQGKKDTKKKNVQGAKNGGRDDDLFGTNTDLSDRRESQFDDSDEEEEEFSEFYNSKAP
                     WDFTLAYSLTYSNTNRESTISGNSVMFSGNIDISPRWKVGVSSGYDFVQKGVTFTQFR
                     FERDLLSWRMDFNWSPYGANAYWGFYIGIKSSVLSDLKYDKRSIPDKTLR"
     gene            18955..19341
                     /locus_tag="P5335_03475"
     CDS             18955..19341
                     /locus_tag="P5335_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRZ73973.1"
                     /translation="MKKIIFTDKAPAPIGPYNQAVLIGNTLYTSGQIALNPKTGELIL
                     DDIETETNQVMQNLKAVLEAADMNFDNVVKTTIFIMNMEDFAKINSVYAEYFNEKTAP
                     ARETVQVAALPKFVNVEISMIAIKEY"
     gene            complement(19338..19709)
                     /locus_tag="P5335_03480"
     CDS             complement(19338..19709)
                     /locus_tag="P5335_03480"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HRZ73974.1"
                     /translation="MEIAIIAHDGKKADMVQFLNKNRAILMQENIKLIATGTTGSKAE
                     NAGFKVKKMLSGPQGGDAQIAGRVAEGKTKMVLFFKDPLSSHPHEADVSMLIRVCDVH
                     NVPLATNEATAQLLLLGITAV"
     gene            complement(19793..20644)
                     /locus_tag="P5335_03485"
     CDS             complement(19793..20644)
                     /locus_tag="P5335_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HRZ73975.1"
                     /translation="MKLLVDGGSTKADWIAIDENGKVLFTTQTLGLNPEVLDKEEVIA
                     RLEDKFDISHNRTKAKNLYFYGAGCGTDRMKNYLAEVFQEYFPNATVAVYEDTYAAVY
                     ATTPKDEEAIVCILGTGSNCSYFDGKILHQKVQSLGYIAMDDGSGNRFGRHLIRGYYF
                     NKMPKELAVEFEEEYNVEPDYIKANLYKEANPNAYLATFAKFIIKHKEKEFCKKIIYK
                     ELTKFVKNYIMQYENCKTVPVHFVGSIAFYLKDELTEILSKYDIKIGNVLRRPIDGLI
                     AYHVLNK"
     gene            complement(20713..21720)
                     /gene="gap"
                     /locus_tag="P5335_03490"
     CDS             complement(20713..21720)
                     /gene="gap"
                     /locus_tag="P5335_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRZ73976.1"
                     /translation="MSKVKIGINGFGRIGRIVFRESYNRDNVEVVAINDLLEVDHLAY
                     LLKYDSVHGRFNGKVEVKDGQLYVNDKFIRVTAERDPKLIKWDDEKVDVDVVAECTGI
                     FTTLATAQAHIDGGAKKVVISAPSADAPMFVMGVNHLQAKASDTIVSNASCTTNCLAP
                     LAKVIHDNFGIVEGLMTTIHATTATQLTVDGPSKKDFRGGRSALLNMIPSSTGAAKAV
                     GKVIPDLNGKLTGMSMRVPTADVSAVDLTVKLAKETTYDEIMAVLKKASENELKGILG
                     YTEDDVVSQDFVSDSRTSIVDAKAGIGLNSTFFKIMSWYDNEYGYSSKMIDLSVHIAK
                     L"
     gene            complement(21760..>22065)
                     /locus_tag="P5335_03495"
     CDS             complement(21760..>22065)
                     /locus_tag="P5335_03495"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRZ73977.1"
                     /translation="GDKIGKSVFELKDYIDQNLQEYDVRVSVLGHMQRGGAPSCFDRV
                     LASRLGVKAVESLLENKTGYMVGLHNDKIELTPLEQAIKGHSGIDRELLRVSDIMSV"
BASE COUNT         7211 a         3661 c         3211 g         7582 t
ORIGIN      
        1 aattgtctac aaactgtttg tattttgtga aagttgtccc tttaagaatg ttatgcccac
       61 catatcgttc atcattaaac aaagtatgtc caatatgttt catgtgggcc ctaatttgat
      121 gcgttctccc agtttctaat ttacaggata tcaaagttac atatcctaat cgttctataa
      181 ctttataatg agtcacagca ggttttccca tactttcatc ttcaaaaatt gccatttgca
      241 ttcgatcttt cacatgtctg ccaatgtaac cacggactgt accttcctct tcttctacat
      301 ttccccaaac taacgcaata tattctcgtt cagaagtttt agctgcaaat tgcttagtta
      361 aataggccat cgctaaatca gtttttgcaa tcaccaaaag accggaagta tttttatcaa
      421 ttcgatgcac taaaccaggt ctttctgagc tattcatagg aagattctca aaatgataag
      481 ccaatgcatt tactaatgtt ccgctataat ttccatgtcc aggatgaact accattcccg
      541 cttctttatt aacaactaac aattgatcat cttcatagat aacattgatt ggaatatttt
      601 cgggttccaa caagtattca aatggcggat gaggcatcaa aaccctaatt acatctaatg
      661 gttttacttt gtaattagat tttacaggtt gttcattaac caatatattt cctgcagttg
      721 ctgcttgttg aattttattt cttgttgcat tttcgacaac ttgcattaaa tatttatcaa
      781 tacgaagcat cgattgccct ttaggaactt caaatcggtg gtgttcaaat aattcgtcgt
      841 ctgtttcaag ttcgacagtt ggaataatat tattcattag tggggttgct ttcttgttcg
      901 cgaatcaaac tgtctatttc agtttcatca aaagcgactt taccatcacc taaaaccaaa
      961 tcaattttag atgttttcat gattttggtt cctggtttaa caggagctcc cttgtatctc
     1021 atttctaaaa ccatatcttt acctaaataa ggtttgtaag ttacctcccc tacttgcaat
     1081 cctatcgatt ttagcgttgg ttctgcttgt cggaatgttt tttctatcaa gtccggtaac
     1141 actactaaag tataaccaga tgcatttact ttgatataaa tttttctatt tgccttaact
     1201 ttagctcctt ttttgggttc ttgttctaca acagaaaatt gtggaaaatc tttattataa
     1261 tccaacgtat ctaataacac ataatctaaa cctaattcat ctaaagtttc ttctgcctgt
     1321 tcaacattca ttttactcaa atctggaaca gtgatttcct gaccgtgatt agtgaaaaaa
     1381 gagagcatat taaaaaacac aaaagccaaa acagcaataa taccaaatgc tattaaaact
     1441 tgactgaaaa acactttact ggtgaggtaa tttcttaaac tcattctatt ttatttgttg
     1501 caaatatata aaataataaa ccgtttttag tttccataaa taaatgataa atttgttaag
     1561 gtaaaaccta atctttaaac gcaaaccaat gaaaaatgtt gccatcatca tgggcggata
     1621 ttctagtgaa tatcaaattt cactcacaag tggaaatgta gtttatcaat acttggataa
     1681 atcgaaatac aatccctatc gtattcacat atttaaagaa aaatgggttt tggtaaacga
     1741 tgataaaaat gagtttccgg taaataaaaa tgatttttcc gttagtatga atggtgaaaa
     1801 attaaacttt gatgttgttt ttaatgccat tcacggtact cccggggaag atggtttgat
     1861 gcaagcctac tttgaattat taactattcc acaaactagc tgcgattatt atcaatcagc
     1921 tttaaccttt aataaaagag atttgctttc tgtattaaaa ccctatggaa tcaagacggc
     1981 tacttcttat tatttgaatt ttggtgatgc tgttgaaccg aaagcaatta ttaaaacggt
     2041 tggattgcct tgttttgtaa aacccaataa atcaggttcc agttttggaa tatctaaagt
     2101 taaaacgaaa gaagaattaa ttcctgctat agaaaatgcc tacaaagaag ataatgaaat
     2161 cattattgaa agttttttag acggtattga agtttctgta ggtgtaatca attataaagg
     2221 aaaaattact gttttaccaa tcactgaaat tgtttcagaa aatgatttct ttgattatga
     2281 agctaaatat ttaggaaaat cacaagaaat cactccggct agaattagtg aagaattaaa
     2341 agaaaaaatt gaaacggtag caaaaagagc ctatgaagtc ttaaaaatga aaggattttc
     2401 aagaagcgaa tttattatag taaataacga accctatatg ctggaaatga atacaatccc
     2461 tggaatgact actgaaagca ttttaccgca acaagctaaa gttgccggta tctcattgtc
     2521 tgatttgttt gacaatgcca ttgaattagc gttgacaaaa taatataatt atgaaaaaag
     2581 caatattccc aggttcattt gaccctataa cattaggaca ttttgatatt attcaacgcg
     2641 gtatttcact ttttgatgaa gtaattgtag ccattggagt aaatgcagaa aaaaaatata
     2701 tgttctccat tgaacaacgt atgaaattta ttgaagaagc atttaaagat gagccaaaag
     2761 ttcgggtaat tacctatgaa ggactaacaa tagacctatg taaaaaagaa aatgcagaat
     2821 ttattttacg tggtttacga aatccggctg attttgaatt tgaaaaagcg attgctcata
     2881 ccaaccgaag actgtcaaaa attgaaaccg tatttttatt aactgcttct aaaacttcct
     2941 acattagttc gagtattgtt cgcgatgtta ttcgaaatgg tggagattac accgtattag
     3001 ttcctgattc agtcaagata aaataaaaaa acccgattca atgaaccggg ttttaatgta
     3061 tcatatttaa aatattattc tttgataagt cttttattta aaactccttc tgaagtttgt
     3121 aaattcaaaa tatagattcc tgtattgtat tcagaaatag aaaatgaatt tgaagcatct
     3181 aaattgattg ccatttgttt tcctgtaata tcagttaaag tggatttttc aatggaaact
     3241 gaagctggta aatcaactgt aaattttcct ttagatggat ttggaaaaac agacaccttt
     3301 tgtaaaatac tttctttaac acccaaagta gttccagtaa cactcaatac taaatgagca
     3361 tttggaaaat taattgaagc tagtggtgtc ggagggacaa gtgcacaagc tgttgaagaa
     3421 atataagttg gagctgattc acccaaatca ttagcagaca agaaaattct atttccacta
     3481 ctagccgttt gagctaaata tcctacttca actaatacgt ttgaaccagc cggaattgtg
     3541 gcagcaatag gaactgaaac taacgtttca acatctgcta acaagaaatt tgtggtaact
     3601 tgtgctaatt cagtatatcc tgcaggatat gctactggaa aattattact tgttgtagca
     3661 taaattttaa ctgtaacagg aaaaccatct ggtaaagttg gaatggtcaa ggtctgaaca
     3721 ccaaactgta cactagaaat agtgtaatcg gcggtaattg aaaaatcgct taatttaaaa
     3781 tatctaaaat agcgattatc acttgatgtt gaaggagtac caccattaca agcgacattt
     3841 gtatttgaca aagtcataga ggtagaatga gaaatagttt gactaattcc gataaaacca
     3901 aaaagcaata aaaaaagtaa agtaattttt ttcataaatg tttattttta aatttgttgt
     3961 aacaaaacta attttttttc ttacacaaaa tacattttgg ttattttttt ttaaataaaa
     4021 caaacacatg ttaaaatatt tacatttttc actatttctt atctgctttt tatctttctc
     4081 gcaaaataaa acttccattt tgacaccttt tgaaaaagga aacggcaatc aatcgacaac
     4141 ttatgaagaa tgtatttcct tttataaaaa tttagctact aattattcaa ccattcaaat
     4201 actggaaaaa ggtaaaactg atagtgcaaa atcattgcat attgttgttt tttctgaaaa
     4261 taataacttt gattttgatc aagataaagc aattgttttg gtgaataacg gaattcatcc
     4321 cggtgaaccc gatggtattg atgcttcaat gatgctcctt cgagatttgg caacttcaaa
     4381 aattaaagtt ccaaaaaata cgctagttgt agttattccg gtttataata ttgatggaat
     4441 gttaaataga aattcctctt ccagagctaa tcaaaatggt ccggaagctt atggttttag
     4501 aggaaatgga agaaattatg atttgaatag agattttatc aaatcggaca ctaaaaattc
     4561 aagaagtttt caagaattat ttaggagtat caatcctgat gtttttttag acaatcatgt
     4621 ttctaatggt gctgattatc aatatacttt tacttgtatt gctactcaac acgaacgatt
     4681 aggaacaact ttaggaaact atttcaccag tgaatttttt cctgacattg tcaaaagcat
     4741 gaaccaaaag aaaattgatg tagttcccta tgtaaatgtt cacagtacta ctcctgacca
     4801 aggttatgaa caatttacag acacaccaag atatgctact ggttatagct cacttttcaa
     4861 taccttgggt tttgtgcctg aaacgcacat gctcaaatcg ttcaaagaaa gagtgaaagt
     4921 aacttatgaa tttatggtta gtgttataaa ttataccgat gccaattgga ataaaattaa
     4981 aaaaatcaga acgaaaagtt ataatgatta ccaacaagga acaaactatc ctattgattg
     5041 ggcaattgac tcaactaaag tatcatacat tgattttaaa ggatttgaag gactgaaaaa
     5101 gccaagtgac atttccagga aagatcgatt atattatgat agaagcaagc cgtttactaa
     5161 gaaaattcca ttttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctaatt
     5281 caaaaggata cattaattga agttgaaacg tataaaattg acagttatca aacatcaaaa
     5341 tctccttatg aaggtcatta tgtacattac aatactaaaa tttcaaaaag taataaaaaa
     5401 gtaaaattca attctggtga ttatatcttt ttctcaaatc aaactggtgt aaaatattta
     5461 ctagaaacac ttgaaccgga ggctattgat tcctatttta attggaattt ttttgatgcc
     5521 attttgcaac aaaaagaatg gtattctgct tatgtatttg aagatttagc tatggatatt
     5581 ttaaaacaaa atccaatttt aaaagaaaac tttgaaaaaa agaaaatgga ggaagaaaaa
     5641 tttgctgaaa gtggttcggc tcaattagat tggatttata gaaattctat ttactttgaa
     5701 tcctcttatt taacttatcc tgtttataga gtgttgaaat aattttattt attcaaataa
     5761 taattttatg ttttcataag agtttgtcaa agcaacttga atctcctttg gagttttcca
     5821 ctccactttt tcaatccctt cttcgacttg accaatcaat ttttcattga aactcgttcg
     5881 catttcaaac caatgggtaa ttttcagttt ataacgtccg tttcgtttaa aaatatgata
     5941 tgttttagga agctttctaa ttatttttaa tcctttcact cctgtttctt cttcaacttc
     6001 tcgaatagcg gtatcttcaa tttcttcacc tttttcaatt ccgcctttag gcaaatccca
     6061 ttttttattt ctaaaaataa acaaaacttc atttttatca ttgtaaacta atccaccccc
     6121 agccttttca accgggattt ttgatttcaa ctttttcatc atttcctttt catccggatg
     6181 atataaaaaa gctttatcaa ctttattttt aaatattttg ataataagct ctttaatatc
     6241 aacactttca agtaaaaaca attgaaaatc cgtttctttt tggatttcat ttgtcaaaaa
     6301 aagtggcttg tcgttcacaa aaactttata catttgtact atgattttaa ataaagatac
     6361 agctaaaaaa acggccgaat tgcttttaca aataaacgca attaaattaa atcctaaaaa
     6421 tccttttaca tgggcttcag gatggaaatc gcctatctat tgtgataatc ggataacact
     6481 ttcatttcca ccaattcgta attatttacg agaggaattt tctaaacata tcgaaaaaca
     6541 atttggaaaa ccggatgtga ttgccggtgt tgccacaggt gcaattggaa ttggaatgct
     6601 tgttgctgaa tacttggggt taccttttgt atatgttcgt cccgaaccta aaaaacatgg
     6661 tagacaaaac caaattgaag gctttttaca aaaaggacag aatgttgttg ttgtagaaga
     6721 tttaatcagt acaggaaaca gtagtttact tgctgtggaa gctttaaaag aagcaggagc
     6781 taatgtaaaa ggaatggttg ccatttttac gtatggtttt gatgtatctg ttgataactt
     6841 taaaaaagca aatgttgatt tatacacgct ttcaaattat gataatttat tagaattagc
     6901 tgtagctaaa aattatatca cagaagaaga acagcacact ttacaagaat ggcgggttag
     6961 tccatctact tggggagttg aagtttaaga atagaaaaaa gagaaaagaa aaaagaaaaa
     7021 agagaaaaga caaaaatcta aaccgacatc ggaaaacaga caaccgataa cagaaaacaa
     7081 aaaaatgaat ttagaaagcc caaaagttac tgtagaaaaa tcagctcagt atttatttga
     7141 ttccttgagt gatgttcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
     7261 tgacggtgat tttaatccta tgatggctat gatgattaaa ggacctattt caaaatttat
     7321 agaaacttta gctgcaaaca tgcataaatt ataaaactac tttataattg catcataaat
     7381 aacctgctga atactttctc gaatattttt ttgagataat atgtttttct cagcattcgg
     7441 aaaagttcta tttgataaaa agatataaat aatttcctcc tcaggatcag cccaagccat
     7501 tgttccggtg aaaccggtat gaccaaaact agtcatcgaa gcacaaccgc atgttggtcc
     7561 ggatgttcct aattgcggct tatcgaaacc tattccgcga cgatttccat tctcgcaata
     7621 ataacatgta ttaaaggctt caaacgtatt tgctgaaaaa taaacttctc ctccatagct
     7681 tcctttttgc aaaaaaagtt gcatcatctt tgctaaatcc attgaattgg aaaagattcc
     7741 ggcgtgccct cctactccac cttccattgc tgcctccatg tcgtgtacat agccttgaat
     7801 agtttgatat cgaaaataag tatctatttc tgttggtgga attttactca tatcaaactt
     7861 gtgcaacgga ttgtataacg tattattcat acccaattta gaataaaagt ttttactact
     7921 caattcatca agcgtttttc cagttgtttt ttctaaataa tttttaagta tgataaacgt
     7981 aaaatcactg tatttatatt ccttttttgg taataattta ctttctgcaa tagccttcat
     8041 gatggtgtca tgatagtctt ttcttaaata taaattatca gctacttgat ttggaaattc
     8101 agtgctatat gcttttctgt aatacttctc ggacggcaag ttatttgaat ccaatgtttg
     8161 tttataaaat ggaatccatg cctgcaaacc tgcgtaatgt gataataaat ctttaaaaat
     8221 gatgttttct ttatctgttc ctataaaaag tggtaacatt tcacccaatt gggtttccaa
     8281 agttaccttt ttattatcgt ataattgcat cacattaggc aaagtagata caatttttgt
     8341 caacgaagcg acatcataaa tatctgaatt tttaacttgc atttgttttt cataagtatg
     8401 aaacccaaaa gctttttgat atattacttt tccttttctg gcgactaaaa tttgtcctcc
     8461 cggtgtcatt ttttcatcta ttgctttttg catgattgca tcaattcgtt gcaaaactaa
     8521 aggattcatt tttacatttt gtggaggtgt aaatcctaaa cgatttaatt tttcagttga
     8581 taaaccatgg ttaactttaa attcttcatt aatagtaacc ggtaattttc ccttagcctc
     8641 aatagcacca aaaataagtt gggcagaaac attttgagcc acatcgctat tttgataaga
     8701 aactataaca ccatcaatat tttcaaaagt aggaattgaa cttaacgcat agggttttac
     8761 aaaacaatct aaaattactt ttttacgttt ggctatttct gtaatccaaa caatttcttt
     8821 agcagaaaaa tcatgttttt tccaagcacc atctgcttta tgaaatccta aaataacctt
     8881 tgtataatct cttaataaaa catttaatga atctatattt tcatgtttaa cctcagttat
     8941 atcagtatat ttttttaatg tttctaaaaa aacagtatta gaatcatctc ccaattttac
     9001 gtaagcaatt ttatctttgt ccaattcttt aattggaata atggctcctt tatttttaat
     9061 tactgtaata gcattttcat acaaatgata ttgtaatcct tcatcctgag gagtatttat
     9121 ggcagatgaa agtttttgaa tttcaattgg tttattggca ttcaatccgg ctttgtactt
     9181 gaaatgcaat atttttttaa cggaatatga caatctatct tgtgtaatta atgcactatc
     9241 tatggcctct tttatttttt gaattgcgac aggaacattt tcaggaaaaa gcaaaatatc
     9301 atttccggct gtaaaagcag ctaaatcaat atcccccggt tttttaaaat tgcttgctcc
     9361 tttcatattc agagcatctg taaaaattaa ccctttaaaa ttcaactctt tttttagcaa
     9421 atcagtaacg ataacattag aaattgaaga aggataaccc ggacgatttt caaaacttgg
     9481 tacatcaaga tgagcaacca ttacactggc taatcctttg ttaattaatt tcttataggg
     9541 atacaattca atatctcgta tatgttcctg tgaaaaattt actaccggaa gcatatgatg
     9601 tgaatctgat tctgtggcac catgacccgg aaaatgttta ccggtggcaa atacgccatt
     9661 agattgtaat ccaatcatta gagctaaagc tctttctgcg acattctttt tatcttctcc
     9721 aaaagaacga tttcctatta ttgggttttt cggattggta ttgatatcta aaacgggtga
     9781 aaaattaaaa tgtactccca attgtttaga ttgaatagcc attaattccc ccgttttttc
     9841 aattaaattt aaatcttgaa tagcacctaa agtcatattc caaggaaaac gattgacaga
     9901 atccaaacgc atacttaatc cccattcggc atcaatacca ataaacaaag gaactttcga
     9961 tttcttttga aaacgattag tcaattgagc ttgacgaacc ggtccgcctt gaaaaaaaat
    10021 taagccccca attttttgtt cttcaattaa tttatcaagt gatttaatat gagttgaatc
    10081 tttatttgaa taggcggcca ccataaatag ttgaccaatt ttttcttctg tggaaagtga
    10141 actaaaaata ctgtctaccc actttttttc ttcagggctt gacaaggaat aattgaacgt
    10201 taaattttca aaatcctcta tatttttatc tgtggtaggt ttactagttg attctgaaat
    10261 aaccgcatta gagttggcag ctttttttgt agcacaattc acaactgaaa tcagcaaaaa
    10321 gaggagaaaa aaaattcgaa gaaatttgag cattatattt attttagaaa aatcggctat
    10381 gccagctatc tgaggcagga acttcccagt tttctttcca ttcttcaatt gtgttaacaa
    10441 gattattaaa tacaattgta ttttcagaaa cagctttttc tttagccatt cgtttaaaat
    10501 ccagcaatga tttatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntttc
    10621 agaaacagct ttttctttag ccattcgttt aaaatccagc aatgatttat gagtcaaaaa
    10681 ttctccttgt ttgtaagtaa cgttcatttt gtccaataaa tctactttgt atttttgttc
    10741 taatttttga atgaactcaa ccgatttatc aattgaacat cccgttgctt gttgaacttc
    10801 ctgattaacg gccaaaatta gaaatcgatt atattttgtt ataaaagagg cttctaacga
    10861 agttccgtga gactcccatt catcaataaa cgattgcaaa tctatctcta tttcagttag
    10921 ttcatcatcc gaaaattttc ggttggattg ataaatccaa attctggatt cttcgggaag
    10981 actatcaaaa ggaatataca tttttttagg gttttgggtg attggtaatg ggttttagaa
    11041 ctatttcctt ataccaatta ccatttacct attatcgaat attataaatc ttgtgcattt
    11101 gctattaact cggcaacatc cataactttt acggaggttt ctttttcttt gtttttaata
    11161 ccatctgtca tcatggtatt gcaaaacggg catccggcag caataatgtc tggttttgtt
    11221 tctaatgcat cttccgttct ctcgatgttg atttcttttt taccgggttc agcatcttta
    11281 aacatctgtg cacctccagc accacaacac aatccattgg ctttggaacg tttcatttct
    11341 actaattcaa cttctaattt ttgaattaat tctctgggtg cttcatacac attatttgct
    11401 cttcctaaat aacaagggtc atgaaaagtt attcgctttc ctttaaattg accaccttca
    11461 attgttaatc gaccatcttc taaaagtgac tttagaaatt gagtatggtg catcacttca
    11521 tattgtccac ctaattccgg atattcattt tttattgtat tgaagcaatg tggacaagcg
    11581 gtaacaattt tcttaacttc ataagcattc atgacttgaa tattcatcat agcttgcatc
    11641 tgaaacaaaa attcatttcc ggctcttttt gccggatcac cagtacaact ttcttcggtt
    11701 cctaaaactg caaaagaaac attcgcccga tttaaaattc gaacaaatgc ttttgtgatt
    11761 tttttagctc ggtcatcaaa acttccggca catcctaccc aaaataaaac ttcaggttgt
    11821 ttgccttgag ccatcatttc tgccatcgtt ggcactacta aattttctgc catgttattt
    11881 tgtttatgac aacactttca ttgtcagtac tattcgtgat gttcgtcgtc aaatacttga
    11941 atggtaactt ctttttcaac taaatctgta aattttcctc tgtaacgagt tgcttttact
    12001 aaatgattat caatccaatg gtaatttcca ccacgcggtt taccaaataa aattccgtgg
    12061 tatttgaatc catgtttttt aagccaaatt tcagtatatt ctctgtgagc ttcagttctg
    12121 gaagtaaaaa agaatataat atgaccttca tcataccatt tatttaatgt taccaaagca
    12181 tccggataaa cctcggctgt taacatacgt tccggttctt cgttgggaat atcatcacat
    12241 acagttccgt caatatctat taaataattt ttaattcctt ccggtaaaat cggacttaaa
    12301 tgttgtccat tttcagtaat ggcttgaagc tttttgttaa tgtcttccgt tttcattttt
    12361 tagtttttaa ttaattcaca ttcattaaac acgttcccaa gcctcactat tatgggaatt
    12421 tgttttttta tatttagttt agttctcctc tttccaattc aagcgatcca tttggttata
    12481 ttgccatgga gcaccattat tttcaatatt tgccatcata gcgtttattg gcatcggtgc
    12541 agcactttgt tccatgacca aatatcgtct catatccatg atgatagata acgggcttat
    12601 attaacaggg cattcctcaa cacatgcgtt acatgaagta catgcccaaa gttcttctgt
    12661 agtaatgtaa tcatttaata aagatttatt atccgacaga aaaacaccat tgttcaaatc
    12721 aatatttttt cctacttctt ccaaacgatc acgcgtatcc atcataattt tacgaggaga
    12781 tagtttttta ccggttagat tggccggaca agaagaagta catcgtccac attctgtaca
    12841 tgtgtatgca tttaataatt gcacccaatt taaatcctgc acatcactag ctccaaattt
    12901 tgccggaaca gtctcaggat ttgccggtgc agcaaatgga tcagctgaag gatccatcat
    12961 taatttaact tcggttgtaa cactttccaa attatcaaac tttccttttg catttaaatc
    13021 agcaaaataa gtatttggaa atgccaataa aatatgtaaa tgtttcgaaa aatataaata
    13081 atttaagaaa accaaaattc cacaaatatg taaccaccaa gcagttcttt caattacgac
    13141 tactactcct tctgacaaac catcaaatag tggcgagata aattgactga taggaaaact
    13201 acctgcttgg ttgtaatgat gtacattcat ttcctgtagc cataaatccg ttgcattcat
    13261 aattagaaat aatgacatta aaattatctc aaaatataaa atatagtttg catcactttt
    13321 gggccaattt ttcaaatccg agctaacaaa acgttttaat tttattacat ttcttcttaa
    13381 ccaaaagaca ataactgaaa ctaaaactaa aacagcaagt atttcaaatg aagcaattaa
    13441 aacattataa aatccaccca tgaatgaaaa cactcgatgt gtgccgaaca agccatcaat
    13501 aacaatttcc aatagttcta agttgataat tatgaaacca acataaacta taatatgcaa
    13561 aaatcctgca attggacgtt tcaccatttt tgactgtcct aaggcaatca tggtcatatt
    13621 tttccatcgt tcagatgaat tatctgttct gttaacttgt tgtccgagtt taatgtttcg
    13681 aacaattttt ttaacattaa gggcaaaata accaaagcct aagcttaaga ttatcaaaaa
    13741 taaaatatta tctaaatagc tcatttactg ctaattttta attttagtag aatctatttc
    13801 agttggagca acatagggtt tgtttttctt accaaatagt gaaacattga catatcgagt
    13861 tggattcaat cgtaagtctt ctaacagaag ttctaattct ttggaagctt ttgtgaaatt
    13921 tgaatacatt ttgtcatctt tcaataattt acccatagtg ccatttccat tctctaaatc
    13981 ggccatgatt ttatcaatat tggccaatgt tttttctaaa ttttgaacgg tttgcttaat
    14041 attaacttga gcaagagaat cagaaatttt agcaaagttt ccggatgttt tttcaaaatt
    14101 tgctagtgaa ttattaattt ttgatttgtt atccgtcaac ataatattca ctgatttcga
    14161 aatatcacta aattcagcca tagtttcact aaattttgaa atgctgtttt tgatattttg
    14221 ctttgtgtta gcatctaaag tttcattcaa attgcttaac aagatatctg ttgacaccat
    14281 tactttttca attttttctt gtagtggttg taatttttct gaaacaagtg cggttaaacc
    14341 acttttgata tctcctttca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 ggttgtaatt tttctgaaac aagtgcggtt aaaccacttt tgatatctcc tttcaaataa
    14521 tctcccgatt cggctaatac agatggattt tcaattcctg gaacaatttg aatcatcttt
    14581 cctcctatta atccgggttc ataaattaat gcaatgctgg atttaggaat cgaatattct
    14641 ttattaattt gaatttcaac cataggtttc catgaactca tattaatgaa actaatattt
    14701 ttcacttttc caataacaag accattaata gtaaccgggg atgaatttac caaaccttct
    14761 acttcattgt attctatata aaaagtatta taatctgtta agatatcggt tccttttaaa
    14821 tagctataac cccaaatgaa taacaaaatg gaggaaatta ctagtattgc tgtttttatt
    14881 tctttcgtta atttcaaaat tgttctagtt tttagcaaat ttaagataaa aaatatttaa
    14941 ttagctttta atgcttcttg aatgctaatt ttatttccat tcttaaaggc aacaataaaa
    15001 gaggattcat atcctttatt ttttgcttcc gatagatatt tcttagcttc gtcatagtta
    15061 gaggtttgag aaaaataata tttatacaaa cccttttcgt caataactga aattggagat
    15121 aatccattaa aattctgtgg aactaaatct agtttttttc cactggctga tatttgtatt
    15181 ttaaaagtaa ttcctttact gtccgggata ggctcattag taataacttc cttttgtttt
    15241 tcaacttctt tggtattgct tttattttga acaactggag tatctttctc tactttttca
    15301 tctgtcactt tacttggttt ttcatcaata ataacattgg tgcctccaaa atattctttt
    15361 ttataattca aaatagcatc tgaaatacct ttggctaatt cttcctgacc tttatcagaa
    15421 tttagatatt ctccttcagg cttgtaagat aaaaaaccta attcggttaa tatactaggc
    15481 atggaggttt gattcaaaac taaaaaagga ccttgtttaa cacctctgtt ttttctgtct
    15541 aatttattag taaattcatt ttgaatttta gtggaaagtg ttatgctttg gtctaaatat
    15601 tcttcttgca aaagtgtaat tccaattaca ctctccggtg aattaggatt atagccggca
    15661 tattttaatt tataatcgct ctctagtgta ataacagcat tctccttttt tgctacttcc
    15721 aaattcgaag catttttggt taaacccata acaaacgtct cagttccata agcatcgcct
    15781 tttggtgaag cattacagtg aattgacaag aaaatatttg catttgctct gttagctatt
    15841 ctcgcacgtt ccatcaactc aataaaaaca tcggtatttc gagtataaat tacttgaata
    15901 tcgttgtgct tttctaaatt tcttccaact ttcaaagcta cttttaaagc aatatttttt
    15961 tctatgaaac cataataatt agcaccaaaa tcttttccac catgaccagg gtccaacaca
    16021 actttaaatt ttgtatttgt ctgggaaaat aaatttactg ttgatagtaa tagaacagca
    16081 aaaaagataa atttgaattt atttaaaaag tacatcattg taaaagttaa tttaattaaa
    16141 actctaggtt ttaaatttta ttatttactt atttttgacc gaaaattata tgtaagtttg
    16201 gcactccaaa aatcaagcca taattttaca aaaatagcat taaaacattt gcggacaaac
    16261 ttatttcata tcgttttatc agcatttttc ctaacaattg gatttccaaa agttagtgca
    16321 caggaattgc aaaataaaac cttaaaggtt gaagctcaaa aaccagttga tagcctgatt
    16381 atcaacaatg aagatttttt tcagattaaa gacacggtaa ttaacgattc tttacagcct
    16441 aacaagaaaa aaccgctttt agaagataaa atcaaatata aagctgataa atacactaaa
    16501 attgatcaaa agcagaaatt aatcacttta tacgacaaag ctgaacttta ttatcaagat
    16561 attgaactga aagccggaat tattatcatg aactatgaaa aaaacgaagt ttatgccggc
    16621 agaattaaag attcaagtgg agtttatacg caaaatcctg tttttaaaca aggttcaaat
    16681 gttgtagaac ctgattccat tcgttttaat tttaaaactc aaaaggctct tatttggaat
    16741 tccagaactg aacaaggaga atttagagtc aaggcagaaa tttcaaaaaa agaaaatgac
    16801 tcagtctatt ttttaagagg tgcccgattt actacctcaa aagatattga taatcctgag
    16861 tattattttt atacaaataa aattaagtta gttccccgaa aaaaagtagt tactggtttt
    16921 accaatatgg ttatagccaa tgtgcccaca cctatagcaa ttccattcgc ttttttccca
    16981 atgacagaag aaagtagttc cgggttaata attcctactt ttcaagatac ccgtagtcaa
    17041 ggttattcgt tacaaaatgg aggttattat ttagctttga gtgactatta tgatttagcg
    17101 attttaggag attattatac caatggtagt tggggattgc gatttcaaag tagttatgct
    17161 ttaaaatata ggttcaatgg aagttttaat ctccgttttg aaaacattat acaaagtgaa
    17221 cgtggatttc ctgattattc aaaatcaaaa aattacaata ttcaatggtc tcatacgcaa
    17281 gacgcaaaat caaatccaaa ctcacggttt tcggcatctg tcaatttagg tagtagcaaa
    17341 tattttcaac aatcactaaa tcagataaat gttggatcaa atttggtaaa caatttgagt
    17401 tcatctgttt cctactctaa gacttttaat actgttccgc aagtaaatat gtccctatct
    17461 gctacccata accaaaatac aggaacagaa gaaattaaca tgacattgcc tactttacaa
    17521 gccagtgttg accgaatttt tccatttgct tcaaaagaag gagccaaaaa aggattaatt
    17581 aaaaacatca atcttcaata caatttaaga ggtgaatatc gaatcaatac ctacgattca
    17641 ttgtttttta aaccggaaat gtttaaagaa gcaaaaaccg gaatgcaaca caccattcca
    17701 ttaagcacaa attttaaaat ttttaaacac tttagtgttt ctgccaatgc aaattataat
    17761 gaagtttgga ctttaaaaac gattaataaa gcctttgatg aggaattaaa taaagtggtt
    17821 actaatgaag tcagtggttt tgattcctac cgaacttact cttttggatc aaatttagga
    17881 acaacaattt acggaacatt taattttggt gaagctaaaa agattcaagc tatcagacat
    17941 gtgatgcgac cttccgtttc gtatagttac acaccaagtt ttgaaaaata ttttgatact
    18001 tatgccattg atgccagcgg aaaaatggat gattacaccc gttttgaagg tggaattttt
    18061 ggtgaaccgt ccaatacata ttccaatttt gcaggacttt cattaggtaa tacatttgaa
    18121 gcaaaggtga ctgaaaaaga cagtaccaaa acagaaccca aaaaagtaat gttgcttaat
    18181 caattgaact tttcaaccag ttataattta gcttctgatt cattacaatg ggctcctgtt
    18241 agaatgagcg gaggaacatt attattcaat aataaaatga acgtgaattt tgcggcaact
    18301 ttagatcctt atgccattaa tgctgccgga agaagaattg ataaatttaa tattgataat
    18361 ggtggaagtt tgtttagaat gaccagtgcc aatatgactg ccaattattc tttttcaagt
    18421 caaggaaaaa aagatactaa aaagaagaat gtacaaggtg caaaaaacgg tggtcgtgat
    18481 gatgatttat ttggaaccaa tactgactta agcgaccgaa gagagagtca atttgatgat
    18541 tctgatgagg aggaagaaga attttcagaa ttctataatt ctaaagctcc atgggatttt
    18601 acgttagctt attcgttaac ttacagcaac acaaataggg aaagtacaat ctcgggtaat
    18661 tctgtcatgt tttcaggaaa tatcgatatt tcaccccggt ggaaagttgg cgtttcgtcg
    18721 ggatatgatt ttgttcaaaa aggcgttact tttactcagt ttagatttga aagagatttg
    18781 ttgagctgga gaatggactt caattggtct ccatacggag caaatgccta ttggggtttt
    18841 tacatcggaa ttaaatcctc ggtattgagt gatttaaaat atgacaaacg ctcaattcca
    18901 gataaaacat tacgttaatt ttcttctata gaatttaatt atcaataaaa agttatgaaa
    18961 aaaattattt ttacagataa agcaccggct cctattggac cctataatca agctgtctta
    19021 attggaaata cattatacac ctctggtcaa attgctttaa acccaaaaac aggagaatta
    19081 attttagatg atattgaaac ggaaaccaat caagtaatgc aaaatttaaa ggctgtttta
    19141 gaagctgcgg atatgaattt tgataacgta gtaaaaacta caattttcat catgaatatg
    19201 gaagattttg caaaaatcaa ctcggtttat gcagaatatt ttaatgaaaa aacagctcct
    19261 gccagagaaa ctgtacaagt ggcagcttta ccaaaatttg taaatgttga gatttcaatg
    19321 attgcaatca aagaatatta aaccgcggta attcctaata gtaataactg agcagttgct
    19381 tcatttgtag ctaacggaac attatgcacg tcgcaaactc gaatgagcat actcacatca
    19441 gcctcatgag ggtgactgga caacggatct ttaaaaaaaa gcaccatttt tgttttacct
    19501 tctgctactc taccggcaat ttgagcatca ccaccttgtg gaccggaaag catttttttt
    19561 actttaaaac cagcattctc agctttactt cctgttgttc ccgtggcaat cagttttata
    19621 ttttcctgca tcaaaatggc tctgttttta tttaaaaact gaaccatatc tgctttttta
    19681 ccgtcgtgtg ctataattgc tatctccatt tattttaact actaagtttt aaatgatttt
    19741 tttgtaaaag tcacaaaaaa acctgaattt ttattttcag gtttatatta tattatttgt
    19801 ttaaaacgtg ataagcaatt aaaccatcaa ttggccgtct taaaacgttt ccgattttta
    19861 tatcatattt tgataagatt tcagtcaatt catctttaag ataaaaagca atggagccta
    19921 caaaatgaac cggaacagtt ttgcaatttt catattgcat aatatagttt ttgacaaatt
    19981 tagttaactc tttataaata atttttttgc aaaactcttt ttctttatgc ttaattataa
    20041 atttggcaaa agttgctaaa tatgcattcg gattagcttc tttatataaa ttagctttaa
    20101 tatagtctgg ttctacattg tattcttctt caaattcaac tgctaactct tttggcattt
    20161 tattaaaata ataacctcta attaaatgtc ttccaaaacg atttcctgaa ccgtcgtcca
    20221 tggcaatata ccctaatgac tgtacttttt gatgtaaaat tttaccatca aagtaactgc
    20281 aatttgaacc agttcctaaa atacaaacaa tggcttcttc gtctttagga gtcgttgcat
    20341 aaacagccgc ataagtatct tcataaaccg cgacagtagc atttggaaag tattcttgaa
    20401 aaacttctgc gaggtaattt ttcattctat ctgttccgca tccggctcca taaaaatata
    20461 gatttttagc tttggttcta ttatgagaaa tatcaaattt atcttctaag cgggcaataa
    20521 cttcttcttt atctaacact tccgggttta agcctaaagt ttgagtagta aaaagcactt
    20581 ttccgttttc gtcaatagca atccaatctg ctttagttga accaccgtct actaataatt
    20641 tcattaggtt gagtttgttt gattataaaa aaagtcccaa tatattaaat ataatgggac
    20701 tttttattaa aattatagtt ttgcgatatg tacagataaa tcaatcattt tgctggaata
    20761 tccatattca ttatcatacc aagacatgat tttaaagaaa gttgaattta aaccaatacc
    20821 tgctttagcg tcaacaattg aagttctgga atctgaaaca aaatcttgcg aaacaacatc
    20881 atcttcagta tatcctaaaa ttcctttaag ttcattttca gaagcttttt tcaaaactgc
    20941 cattatttcg tcataagtag tttctttggc taatttaacc gttaaatcta ctgctgaaac
    21001 atcggctgtt ggcacacgca ttgacattcc ggttaatttt ccattcaaat caggaattac
    21061 ttttccaact gctttggcag cacctgtaga agaaggaatc atgtttaata aagctgatct
    21121 accaccacga aaatcttttt tagaaggtcc gtctactgtt aattgagttg ctgtagtagc
    21181 gtgaatagta gtcattaaac cttctacaat tccgaaatta tcatgaataa ctttagccaa
    21241 tggagctaag caatttgtgg tacaagatgc atttgaaaca attgtatctg atgctttagc
    21301 ttgtaaatga tttaccccca ttacaaacat tggagcgtca gctgaaggag cagaaattac
    21361 cactttttta gcaccaccat ctatgtgagc ctgagctgtt gctaatgttg taaaaattcc
    21421 tgtacattct gcaacaacat ctacatctac tttttcatca tcccatttaa ttaattttgg
    21481 gtctctttct gctgttactc tgataaactt gtcgtttaca tataattgac cgtctttcac
    21541 ttcaactttt ccgttgaaac gaccatgaac tgaatcatat tttaataaat aagccaaatg
    21601 atctacttca agtaaatcat taattgctac tacttctacg ttatctctat tgtatgattc
    21661 tctaaataca attctaccaa ttctaccgaa tccattgatt ccaattttaa cttttgacat
    21721 tttgattttg tttaattact attattataa atttattttt tagactgaca taatatcaga
    21781 aactcttaaa agctctctat ctattccgga atgtcctttg atggcttgtt ctaatggggt
    21841 caactcaatt ttatcattat gcaaaccgac catatatcct gttttatttt ctaataaaga
    21901 ctcaacagct tttactccca aacgagaagc taaaactcta tcaaaacaag atggtgctcc
    21961 gcctcgttgc atgtgaccta aaacagaaac ccgcacatca tattcctgca ggttttgatc
    22021 gatgtaatct tttagttcaa aaacgctttt accaatttta tcacc
//
LOCUS       DAOTCW010000024        21612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1290_length_21612_cov_40.543814, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000024 DAOTCW010000000
VERSION     DAOTCW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 21612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21612
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1290_length_21612_cov_40.543814"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1780)
                     /locus_tag="P5335_03500"
     CDS             complement(<1..1780)
                     /locus_tag="P5335_03500"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRZ73978.1"
                     /translation="MDSIMIYVPIVMALIGLAFMAFKRAWVLKQDAGDGKMKEISDYI
                     YEGALAFLKAEYRLLAIFVVIASIVLAGITFLPGVKTHLLIVVAFVFGAFFSALAGNM
                     GMKIATKTNVRTTQAARTSLPQALKVSFGGGTVMGLGVAGLAVLGLTAFFIIFFNILS
                     GGVWQDTDAMTVVLETLAGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPE
                     DDPRNPATIADNVGDNVGDVAGMGADLFGSYVATVLAAMVLGNYVIKDMGGNIVDQGF
                     GGIGPILLPMAIAGFGILFSIIGTMLVKINSNDAKEKQVQGALNVGNWVSIVLTAISC
                     FFLVQYMLPEVMTMKFFGEEAQEISSLRVFYATLIGLVVGGAISSVTEYYTGLGTKPV
                     LAIVQKSSTGAGTNVIAGLATGMISTFPTVLLFAGAIWTSYALAGFYGVALAASAMMA
                     TTAMQLAIDAFGPISDNAGGIAEMSELPKEVRTRTDILDSVGNTTAATGKGFAIASAA
                     LTSLALFAAYVTFTGIDGINIFKAPVLAMLFVGGMIPVVFSALAMNSVGKAAMDMVYE
                     VRRQFKEIPGIMEGTGKPEYGKCVEIS"
     gene            complement(1894..4374)
                     /locus_tag="P5335_03505"
     CDS             complement(1894..4374)
                     /locus_tag="P5335_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRZ73979.1"
                     /translation="MKLYSVLLLFMLFSASLFAQTKVSGVVVDEKNDPIPFANVYFKN
                     SSEGVITNENGRFYLESAKNHAVLVISFIGYTPKELVLEKQVTYNLKIKLAEGETLRE
                     VVLVSGKLSKKNNPAIDILRKIWERKRKNGLRQFEHYQYDKYEKIEFDLNTIDSATMK
                     SKLFKGMEFVFEQMDTSAITGKTYLPIFINESISEVYGNNLTKKEKEITKANKNSGFS
                     NNQQVIAFVKDLYADYDIYDNYLKFFDKDFVSPLSRTGIEVYNYALTDSMFIDNKWCY
                     NIVYYPRRKGELTFKGDFWVNDSTYAIKSINMAVTKSANINWIKEIYIEQEFDVVNDS
                     VFLLKRDHMMSDFAFNKKEESQGVYGKRTTLYKNHKFNEEKPKEFYKEEINFIDNSVY
                     NKPDEFWAENRFESLSKDELGVYEMLDTLQTVKKFKQMYRLVSILGSGYIEFNKFDFG
                     PIFSTVGYSDVEGLRFRVGGRTYFGRNDPWRIQAYTAYGFKDNQVKYGLSGKILVNPK
                     NRLILEGGNRRDIEQLGANLTTTNNVLGRSFASTGLFMSGSNTNLTSINLSTLSMNIE
                     PVKNLTFEFGLTYRTLKSASETFSLDYYTDASRTTVQSDVKQSEMTFSVDYTPGRKSI
                     GYGVERAVVDSPYSRFFVSYNKGFKGVLESDFAYDKLQFYFKKPILVGGFGRLNLITE
                     AGKTFGAVPLGLLSVIPGNQSYFIIDNTFNNLNFYEFVSDQYATFQLEHHFLGRLFSR
                     IPGVRKLNLREIVSIKGVVGSISEENVQLNASTLTYLAPEKPYWEYGFGVGNIFKVFR
                     IDCTWRGNYRNLPDTNNFTIKGSFGFYF"
     gene            complement(4441..5418)
                     /locus_tag="P5335_03510"
     CDS             complement(4441..5418)
                     /locus_tag="P5335_03510"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HRZ73980.1"
                     /translation="MRTIQFREAICEAMSEEMRRDESVYLMGEEVAEYNGAYKASKGM
                     LDEFGPKRVIDTPIAELGFAGIAVGSAMNGCRPIVEFMTFNFSLVGIDQIINNAAKMR
                     QMSAGQFSMPMVFRGPTASAGQLAATHSQSFESWFANTPGLKVVVPSNPYDAKGLLKS
                     AIRDNDPVIFMESEQMYGDKMDVPEDEYTIPLGVADIKRAGTDVTIVSFGKIIKEAFT
                     AADELAKEGISCEIIDLRTVRPMDYDAILTSVKKTNRLVVLEEAWPFASVASEITYMV
                     QEKAFDYLDAPVQRITTADTPAPFSPALLKEWLPNSNDVIKAVKKVMYK"
     gene            5693..6439
                     /locus_tag="P5335_03515"
     CDS             5693..6439
                     /locus_tag="P5335_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRZ73981.1"
                     /translation="MKILVCISHVPDTTSKINFVNNDSEFDTNGVQFVINPNDEFGLT
                     RAVWFQEQQGATVTVVNVGGPDTEPTLRKALAIGANEAIRVNANPTDGFFVAKQLAEV
                     VKQGQYDLIICGKESLDYNGGMVPGMIAGLLGYNFVNACIDIKVDASNAKAIREIDGG
                     KETISALLPLIIGGQKGLVEEKDLRIPNMRGIMTARTKVLTVLEPVGAALNTKAVKFE
                     KPAAKSAVKLVSADNLDELVSLLHNEAKVI"
     gene            6482..7450
                     /locus_tag="P5335_03520"
     CDS             6482..7450
                     /locus_tag="P5335_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRZ73982.1"
                     /translation="MSLLIYAESAEGKFKKVAFELASYAKKVAESLGTTVTAVTINAS
                     DVSELSKYGVDKVLKVTNDKLAAFNAKAYADTIKQAAQKEGAKVIVLSSTTDSIYLAP
                     LVAVGLEAGYTSNVVGLPLSTSPFQVKRNAFSNKAFNTTEISTEVKVLGLAKNSYGLV
                     ESDSSLSTEDFSPSLNDTDFNVKVESVEKVTGKVTIADAEVVVSGGRGLKGPENWGMV
                     EELATVLGAATACSKPVSDLGWRPHSEHVGQTGKPVATNLYIAIGISGAIQHIAGISS
                     SKVKVVINSDPEAPFFKVADYGVVGDAFEIVPKLTQKLKEFKAQNS"
     gene            7579..8202
                     /locus_tag="P5335_03525"
     CDS             7579..8202
                     /locus_tag="P5335_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRZ73983.1"
                     /translation="MSLVKLAIKGISYSQTQNGAYALILNEVDGERKLPIVIGAFEAQ
                     SIAIALEKEIKPPRPLTHDLFKNFADRFDIVVKQVIIHKLVDGVFYSSIICERDKIEE
                     IIDARTSDAIALALRFNAPIFTYKNILDKAGIYLKGNSMEPDNPSGENEGVLSNPETF
                     GLEESTVKDSYSGKSVQELYELLNEAVQNEDYEKAAKIRDEISKKES"
     gene            8247..9905
                     /locus_tag="P5335_03530"
     CDS             8247..9905
                     /locus_tag="P5335_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HRZ73984.1"
                     /translation="MYKNLIFLSIILLSTLGLTGQELEKKWQLSSSKTDFLELKEGGY
                     ELKITSDSIIQKGDYLVQDNYLFLFENGSDAPTKRFTIQTKSDSILTLKSKDKAYSFF
                     ASNKIKTTPTEAKLIASQGLSVTSIWKGILGMLSLLALAYVFSANKKAINWKTVGIGI
                     FAQLILAIGVLKVGFVKSLFEFVGGLFVKVLDFTRAGSEFLLGGMMNVDNFGFIFLFQ
                     VLPTIIFFSALTSLLFYLGIIQIVVKGLAMLLSKLLHISGAESLSVAGNIFLGQTEAP
                     LMIKAYLEKMNKSEILLVMVGGMATVAGGVLAAYIGFLGGNDPQLRLVFAKHLLAASV
                     MAAPGAIVVSKMLFPQTESIDTNVEVSKDKIGSNILDAIATGTTEGLKLAANVAAMLL
                     VFIAFIAMINYGLGKIGSLTGLNEIIADNTPYSKFSLETILGIVFAPLMWLIGVAKED
                     VMLMGQLLGIKLAASEFVGYIQLAELKNAANAIHFTYDKSVIMATYMLCGFANFASIG
                     IQIGGIGSLAPGQRKTLSEFGLKAVIGGSIASLLSATIAGMIIG"
     gene            9923..10747
                     /locus_tag="P5335_03535"
     CDS             9923..10747
                     /locus_tag="P5335_03535"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRZ73985.1"
                     /translation="MKQYHDLVKHVLENGTQKGDRTGTGTKSVFGYQMRFDLNEGFPM
                     VTTKKLHLKSIIYELLWFLKGDTNINYLKENGVKIWDEWADENGDLGPVYGHQWRNWN
                     SEEIDQISELIETLKTNPNSRRMLVSAWNPSVLPDTSKSFAENVANGKAALPPCHAFF
                     QFYVADGKLSCQLYQRSADIFLGVPFNIASYALFTLMIAQVCDLQPGEFIHTFGDAHI
                     YNNHFEQVELQLSREPKQLPKMILNPEIKNIFDFTFDDFTLEGYDPHPHIKGVVAV"
     gene            complement(10806..11102)
                     /locus_tag="P5335_03540"
     CDS             complement(10806..11102)
                     /locus_tag="P5335_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HRZ73986.1"
                     /translation="MAIKKQFIKTKPVCKVTFSIEAKDATTAAVVGDFNNWNPAVGEL
                     SKLKNGTFKATFELPASNSYEFRYLVDGQFVNDTEADAYKWNDFAAAENSVVAV"
     gene            11350..11700
                     /locus_tag="P5335_03545"
     CDS             11350..11700
                     /locus_tag="P5335_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HRZ73987.1"
                     /translation="MDKELHEKYEYARRRLKQKKRLYFHFVLFLIGSLFFLIIEKLFD
                     IQPFEGWYLWAVTIWFFLFILHFIKVYITDRFMNKEWEREQINRLVAKQEQRIKQLEQ
                     KAEDELNTRNNTSI"
     gene            11697..12179
                     /locus_tag="P5335_03550"
     CDS             11697..12179
                     /locus_tag="P5335_03550"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRZ73988.1"
                     /translation="MIIMIAAAAENNALGKDNDLMWHLPDDFKRFKQLTSGHYIIMGR
                     KTFESFPKPLPNRTHIIITRQKEYHPEGTLVVNSIEKALELIPKSQDAFIIGGGEIYK
                     QTLQFTDKIELTRVHQSFDADTYFPEISLASWELIQDEYHPKDEKHSIDFTYQTYIRK
                     "
     gene            12756..13487
                     /gene="ubiE"
                     /locus_tag="P5335_03555"
     CDS             12756..13487
                     /gene="ubiE"
                     /locus_tag="P5335_03555"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRZ73989.1"
                     /translation="MSKTVTPYKNSNLGKKEQVAQMFDAISGNYDGLNRVISFGTDIK
                     WRKKVLAMVKSTNPDTILDIATGTGDLALLMSETNATKIVGLDISAGMLDVGRKKIAQ
                     KNLSGKIEMILGDSEKMPFDDNTFDAITVAFGVRNFETLEKGLLEILRVLKPNGIFVI
                     LETSVPEKFPYKQGYKFYSKNILPLIGKLFSKDNSAYAYLSESASVFPYGEALNNILR
                     KIGFIDVVALPQTFGVATIYSASKK"
     gene            13489..14190
                     /locus_tag="P5335_03560"
     CDS             13489..14190
                     /locus_tag="P5335_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRZ73990.1"
                     /translation="MRKFIILIIILAGYQTKAQFKGTFGKDPIIHLENFDKQRVHWGY
                     FLGFNSLDFKIDQNSPSEEILVNNSIGFNVGLVGDLRLYEFLNLRFEPGLYYTQRDLT
                     FPGFEEKVDYLRQVKSTYIFFPLLLKFSSVRTGNVRPYLVGGFSTALNLSSNSKSTDD
                     NSNNRFRMIQWTNFYEVGLGIDLYLEYFKFSPSIRGVFSLNDELIRDNDPNSPWTSSV
                     ESMKTRGIFINFTFH"
     gene            complement(14177..14914)
                     /locus_tag="P5335_03565"
     CDS             complement(14177..14914)
                     /locus_tag="P5335_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRZ73991.1"
                     /translation="MLSKNQIKRITSLQQKKYRNEYQLFIAEGIKVIQELFQSQYIAE
                     HFYTTNPDLSISAAPIPEIIAESDLKKISCLKTPNDCLAVFRIPKQKPINLTGLVVCL
                     DDVRDPGNLGTIIRLCDWFGVEQLVCSPETADVYNPKVIQATMGSISRVNLVYTDLCK
                     FLENTILPVYGAFMEGKNVYESTLPEKAILVLGNEANGISKLVEAKISSKISIPRFGQ
                     LQKTESLNVATASAILLSEFRRNFSEK"
     gene            14951..17512
                     /locus_tag="P5335_03570"
     CDS             14951..17512
                     /locus_tag="P5335_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRZ73992.1"
                     /translation="MKRISAKISFIILIIVLLYACDSTKRVPNGENLLVHTELIVDGK
                     TVKKEEVINLIGQKPNTQFLKLPFSLYVYNWASPNPDSLFAEKFIKDTLRFQRTSRLL
                     SEKQVYRLGESFWYSGIHKFLKSNGEAPVLIDELRTKKSLYKLKSYYFNEGYFKVTAT
                     EKTDSVGLKKVKSTYTVTLGNPYIIDSITKKIDSPVLDSLYLLTENKSSIKKGEIYNS
                     SKFEEERNRLTAYFRNNGVYNFQQNYISYEVDTISTGDKANINLLIGNYSYRSGDTLV
                     SKPFEIFKISEVNIYTDNPNLKSSSKITDTITYNNFNIYSSDKFKYKPKAITDAIFIT
                     KGSTYSDFRNNLTSRSLANFKIFNYPSILYQVNEKDSLSNTLIANIYLSPRKKYSFLP
                     SVDLTHSDIQDFGITANLSAGIRNVFNGAETFDISFRGNIGSSRDNANPNDQFFNISE
                     IGLDAKLSFPRILFPLNTDNVIPKNMLPSTQISGGYAKQKNIGLDKENLTGSFTYKWT
                     PKRNNSIHVDLFSIQYVKNINVSNYFNVYQSSYDALNSISKNYDVNPDYYDENGTGDL
                     IIESGTDGFLNDVLGDNPTIFPTEDDYNEIKNIDGRKTRLTENNFILSASFNYSTSTK
                     KGILDNNFSVFRTKVESAGNFLSLFSGASNQPTGPNGNKTIFDVEYSQYIKTEFEYIK
                     HFEFRKNSVIASRAFIGVAIPYGNSDNIPFSRSYFAGGSNDNRAWQPYSLGPGSSNAQ
                     NDFNDANLKIAFNTEYRFKLFGQVRGALFVDVGNIWNFLDNIDIEAAKFTGLSSLEDI
                     AVGSGFGIRYDFGFVVARFDIGFKTYNPANEIGDRWFKEYNFSNSVFNIGINYPF"
     gene            17633..18700
                     /gene="fbaA"
                     /locus_tag="P5335_03575"
     CDS             17633..18700
                     /gene="fbaA"
                     /locus_tag="P5335_03575"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRZ73993.1"
                     /translation="MAHNIKPGVATGDQVQEIFSYAKEKGFALPAVNVIGSSTVNGVL
                     ETAAKLNAPVIIQFSNGGAAYNAGKGLTNEGERAAILGAIAGAKHIHTLAEAYGATVI
                     LHTDHCAKKLLPWIDGLLDASEKHFAETGKPLFSSHMIDLSEESLEENIHICKGYLER
                     MSKMGMTLEIELGVTGGEEDGVDNSDVDSSKLYTQPAEVSYAYEELMKISPRFTIAAA
                     FGNVHGVYKPGNVKLTPVILKNSQDYVQHKFNTGPNPIDFVFHGGSGSTLEEIREAIS
                     YGVIKMNIDTDLQFAFTEGIRDYMVNKIDYLRTQIGSPDGADSPNKKHYDPRKWVREG
                     ELTFNKRLEQAFADLNNVNTL"
     gene            18771..19625
                     /gene="accD"
                     /locus_tag="P5335_03580"
     CDS             18771..19625
                     /gene="accD"
                     /locus_tag="P5335_03580"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRZ73994.1"
                     /translation="MAWFKRTEKGIQTATEDKKDVPKGLWYKSPTGKIIDQDELAKNL
                     WVSPDDDFHVRIGSKEYFEILFDDNEFKELDAKMTSKDPLHFVDTKKYSDRLKDAYEK
                     TGLKDAVRTAVGKSKGKDLVVCCMDFAFIGGSMGAVVGEKIARGIDYSIKNKVPFMMI
                     SKSGGARMMEAAYSLMQLAKTSAKLAQLAEAKIPYISLCTDPTTGGTTASYAMLGDVN
                     IAEPGALIGFAGPRVVKDTTGKDLPEGFQTAEFVLEHGFLDFITHRKELKDKVNLYLD
                     LIQNEDVR"
     gene            19897..20646
                     /locus_tag="P5335_03585"
     CDS             19897..20646
                     /locus_tag="P5335_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73995.1"
                     /translation="MARQKGIIKLEGTLDGLNFYNRLGEPLSRIAGGGFNSVAIKTKP
                     SMSRVRENGSEFKTCMTSVKYFKLGLQPISYQFKDTLLHQRLVQLFTRIKVFDSVSER
                     GQRTVGIGLQSAAGKKLLHRYVLTSGDGLDSVLRQGFEADWQLNGFRIAAFDMAAVRF
                     PKGATHVSLQAGYLVFDFTTFTPVLAKSAPVVLSKATATAPLELGVSALPVGAGTKVM
                     VVYLQFMQEVNGFMYPMKELNTRVFEVVYVD"
     gene            20988..21512
                     /locus_tag="P5335_03590"
     CDS             20988..21512
                     /locus_tag="P5335_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ73996.1"
                     /translation="MTKNILFSICIIFCISCRKEVEVTQKSAYQDIKFIFPDTVYVNE
                     SYDGQISFKSDFDTITTSLSDYQKARFLEYSFWLTKKPIEDNKLLKEMVTDTFVAETN
                     RVIPLYDIKFDKLGVNYFNGIIKDEIIIAKGGILKDGSKGDRIITNEIRLTRAVYVID
                     NRADTSRSAFYDKN"
BASE COUNT         7307 a         3596 c         3769 g         6940 t
ORIGIN      
        1 tagaaatctc cacacatttg ccatattccg gtttaccggt tccttccatg attcccggaa
       61 tttctttgaa ttgacgacgt acttcataaa ccatatccat agctgctttt ccaactgaat
      121 tcattgctaa agcagagaat acaaccggaa tcatacctcc tacaaacaac atcgctaata
      181 ccggtgcttt gaagatatta ataccatcaa ttcctgtaaa cgtaacataa gcagcaaata
      241 aagctaatga agttaacgca gcagaagcaa ttgcaaaacc ttttcctgtt gcagcagttg
      301 tgttacccac tgaatctaaa atatctgtac gcgtacgaac ttcttttggt aattcactca
      361 tttcagcaat tcctcctgca ttatcagaaa taggaccaaa tgcatcgata gctaattgca
      421 tagctgttgt tgccatcatt gcagatgccg ctaaagcaac tccataaaaa ccagctaaag
      481 catacgatgt ccaaattgca cccgcaaata ataatacggt tgggaaagta gaaatcatcc
      541 ccgtagctaa accagcaatt acgttagttc ctgctccagt tgacgatttt tgaacaattg
      601 ccaatactgg ttttgtacca agtccggtat aatattccgt aacagatgaa atagctccac
      661 ccacaactaa tccaattaaa gtagcataaa acactctaag tgaagaaatt tcttgagctt
      721 cttctccgaa gaatttcata gtcattacct caggcaacat atattgtact aagaaaaaac
      781 aagaaatagc agttaaaaca attgaaaccc agtttcctac gtttaaagca ccttgaactt
      841 gtttttcttt ggcatcatta ctattgattt ttaccaacat cgttccgatg attgaaaata
      901 agattccgaa accggcgatt gccattggta ataaaattgg gccaattcca ccgaaccctt
      961 gatcaacaat gtttccgccc atatctttga ttacataatt acctaaaacc atagctgcta
     1021 agacagttgc aacatacgaa ccgaataaat cggcacccat accggcaacg tcacctacgt
     1081 tatcacccac gttatctgca atagtagcag gatttcttgg atcatcttca ggtattcctg
     1141 cttccacttt tccaactaaa tcagctccaa cgtcggctgc tttggtataa ataccaccac
     1201 caacacgagc aaataatgca atcgattccg ctccaagaga aaaaccggct aatgtttcaa
     1261 gaaccacagt catcgcatca gtatcttgcc aaacccctcc ggaaagaatg ttaaagaaaa
     1321 taataaaaaa ggcagttaaa cctaaaacgg ctaaacctgc aactcctaat cccattactg
     1381 ttccgcctcc aaaagatact tttaaggctt gaggtaaact tgtacgagct gcttgagtag
     1441 ttcttacgtt tgttttagtt gcaattttca tccccatatt tccggctaaa gcagaaaaga
     1501 aagctccaaa aacaaatgca actactatta ataagtgagt tttaacgcct ggcaaaaaag
     1561 ttataccggc caaaacaata cttgcaataa caacaaatat ggctaataat cggtattctg
     1621 ctttcaggaa agctagagct ccttcataga tgtaatctga aatctctttc attttaccat
     1681 caccggcatc ttgttttaaa acccatgctc ttttgaaagc cataaaagct aatcctatta
     1741 atgccataac tattggcaca taaatcataa ttgaatccat aaaaatgtat tttataattt
     1801 ttttaacggt tgcaaaagta acaaaatagg aataaaaaaa agcaacattc ttaatagaat
     1861 attgcttaat agatatataa agtaaaaatt aacttaaaaa taaaacccaa aagagccttt
     1921 tatagtaaaa ttattggtgt ccggaaggtt tctgtaattt cctctccaag tacaatctat
     1981 tcggaatact ttgaaaatat tgcccacacc aaatccatat tcccaatagg gtttttcagg
     2041 agctaaataa gttaatgtag aggcatttaa ttgaacattt tcttctgaga tagaacctac
     2101 aactcctttt atacttacta tttctcttaa attcaattta cggacacccg ggattctgga
     2161 aaataacctt cctaaaaaat gatgttctaa ctgaaatgtg gcatattgat ctgatacaaa
     2221 ttcataaaaa tttaaattat tgaaggtatt atcaataata aaataagatt gatttccggg
     2281 aataacacta agcaaaccta acggaacagc accaaatgtt ttacccgctt ctgtaattaa
     2341 gtttaaacga ccaaatccgc ccactaaaat tggttttttg aagtaaaact gaagtttatc
     2401 ataggcgaaa tcactttcta aaaccccttt aaatcctttg ttatagctaa caaaaaaacg
     2461 agagtacgga ctgtccacaa cagcacgttc cactccatac ccgatagatt tacgaccggg
     2521 agtgtaatca actgaaaagg tcatttctga ttgttttaca tccgattgaa ctgttgttcg
     2581 cgaagcatcg gtgtaataat ccagactgaa tgtttccgag gcagatttca atgttcggta
     2641 ggttagtcca aattcaaacg ttaaattttt caccggttca atattcatcg acaaggtact
     2701 taaattgata ctcgttaaat tcgtatttga accactcata aaaagtccgg tagaagcaaa
     2761 acttctgccg agcacattat ttgtagtggt taaatttgca ccaagttgtt cgatatccct
     2821 tcgatttcca ccttctaaaa tcaaacggtt tttagggttt accaagattt tccccgacaa
     2881 accgtatttt acctgattat ctttaaaacc ataagcggtg taggcttgaa ttcgccaagg
     2941 gtcatttctg ccaaaatatg tacgtcctcc gactcgaaat cgcaatccct ccacatcgct
     3001 ataaccaacg gtggaaaaaa ttggtccgaa atcaaattta ttaaattcta tatagccgct
     3061 tcccaaaatg gaaaccaatc tgtacatctg tttgaatttt ttgaccgtct gcagcgtatc
     3121 taacatttcg taaaccccta actcatcttt acttaaactt tcaaatctgt tttcagccca
     3181 aaattcatcc ggtttattat aaacggaatt gtctataaaa ttgatttcct ctttataaaa
     3241 ctctttaggt ttttcctcat tgaatttgtg attcttataa agagtagttc tttttccata
     3301 taccccttgt gattcttctt ttttattgaa agcaaaatca gacatcatat gatctctttt
     3361 taaaagaaaa acggaatcat ttaccacatc aaattcctgt tcaatgtaaa tttctttaat
     3421 ccagttaatg tttgcacttt tagtaaccgc catattgatt gactttatgg catacgtaga
     3481 atcattcacc caaaaatcac ctttgaaagt gagttctcct tttcgtcggg gataataaac
     3541 aatattataa caccatttgt tatcaataaa catactatcc gtaagagcat aattataaac
     3601 ttcaattccg gtgcgagata aaggactgac aaaatcttta tcaaaaaact tcaaataatt
     3661 atcataaata tcataatcgg catataaatc tttaacaaaa gcaatgactt gttgattgtt
     3721 gctaaatccg gaatttttat tggctttcgt aatttctttt tcttttttag tcaaattatt
     3781 tccatagacc tcagaaatag attcatttat gaaaataggt aaatacgttt ttccggttat
     3841 tgctgaagtg tccatttgct caaaaacaaa ttccattccc ttaaacaatt ttgatttcat
     3901 agtagcacta tcaattgtat tcaaatcgaa ttcgattttc tcatatttat catactgata
     3961 gtgttcaaat tgccgcaatc catttttgcg tttgcgttcc catatttttc gtaatatatc
     4021 aattgccgga ttattttttt tagataactt accggaaacc aacacaactt cacgaagtgt
     4081 ttccccttcc gctaatttaa ttttaaggtt gtatgtaacc tgtttttcta aaacaagttc
     4141 tttaggagta tatccaataa aagaaataac taaaacagcg tgatttttgg cagattcaag
     4201 ataaaaacgg ccattctcgt tagtaataac tccttctgaa gaatttttaa aataaacatt
     4261 tgcaaatgga atagggtcat ttttttcatc tacaactact ccgctcactt ttgtttgagc
     4321 aaacaaagaa gcagaaaaca acatgaaaag taagagaaca gaatataatt tcatagcaaa
     4381 ttaaaaaaaa acttcatcaa aatcgagttg atgaagtctc atatatagta gataatattt
     4441 ctatttatac ataacttttt ttactgcctt aatcacatca tttgaattag gcaaccattc
     4501 ttttagtaaa gccggtgaaa aaggagccgg tgtgtcggca gtggtaattc gttgaaccgg
     4561 agcatctaaa taatcgaatg ctttttcctg aaccatgtac gtaatttcag aagcaacact
     4621 agcaaatggc caagcttctt ctaaaactac taatcgattt gtttttttaa cagacgttaa
     4681 tatagcatca taatccatcg gacgaaccgt tcttaaatcg ataatttcac aagatattcc
     4741 ttctttcgct aattcatccg cagctgtaaa tgcttcttta atgattttac caaaagaaac
     4801 aatggttaca tctgttcctg ctcttttaat atcagcaact cctaatggaa tggtatattc
     4861 atcttctggg acgtccattt tatcaccata catttgctct gactccataa aaattactgg
     4921 gtcattatca cgaatggccg atttcaataa tccttttgca tcataagggt ttgaaggtac
     4981 tacaactttt aaacctggtg tattcgcaaa ccaactttca aaagattgag agtgtgtggc
     5041 tgctaactga cctgcagaag ccgttggtcc tctaaaaacc attggcattg aaaactgtcc
     5101 ggctgacatt tgacgcattt ttgcggcatt atttataatt tggtcaattc caactaatga
     5161 aaagttaaat gtcataaatt ccacaatcgg acgacaacca ttcatcgctg aacctaccgc
     5221 gattcctgca aaaccaagtt ctgcaatagg cgtatcaatt actcgttttg gaccaaactc
     5281 atctaacatt cctttagaag ctttataggc accattatat tctgcaactt cctctcccat
     5341 taaataaacg ctctcatcac gacgcatttc ttcactcatt gcttcacata tcgcttctct
     5401 aaactgaatt gttctcatat agaaattttc ttttaagact ttaaatcgaa acgcaaaaat
     5461 aaaaattaaa aatcacttta aagcatattt ttagtgtaaa ataattggct ttttgtaatt
     5521 tttcattttc aacaatttgt aattttccta ttgaaaaaat ttaattacat aaataaaaga
     5581 attattaata gcaacgaaaa cgatataata ctgaaatttt attatgcacg catactaaaa
     5641 ttagaaatat tatttgtact ttcgtaaccg aaaaataatc taaacctttt ttatgaaaat
     5701 attagtttgc atcagtcatg tgcctgatac tacatccaaa attaattttg tcaacaacga
     5761 ttcagaattt gacacaaacg gtgttcaatt tgtaatcaat ccaaatgacg aatttggttt
     5821 aacaagagct gtttggtttc aagaacaaca aggagcaacc gtaaccgttg tcaatgttgg
     5881 cggccccgat actgaaccaa cattaagaaa agctttagcc attggtgcca atgaagcaat
     5941 tcgtgttaat gctaatccta cagatggttt ttttgttgca aaacaattag cagaagttgt
     6001 taaacaaggg caatatgatt taattatttg cggtaaagaa tcgttagatt ataatggtgg
     6061 aatggttccc ggaatgattg ccggtttgtt aggatacaat tttgtaaatg cttgtattga
     6121 cataaaagtt gatgcatcaa atgctaaagc aatccgtgaa attgatggtg gaaaagaaac
     6181 tatttctgct ttgttaccat tgattatcgg tggtcaaaaa ggcttggtag aagaaaaaga
     6241 tttacgcatt ccaaacatga gaggaattat gaccgctcga actaaagttt taactgtttt
     6301 agaacctgtt ggagcagcac taaacactaa agccgtaaaa ttcgaaaaac cggcagcaaa
     6361 atcagcagta aaattagtaa gtgctgataa tttagatgaa ttagtaagtt tgttacacaa
     6421 cgaagctaaa gttatctaaa catttaatct aatttaaaat ccatcactta aaatataaat
     6481 tatgtcatta ttaatatatg cagaatcggc tgagggaaaa ttcaagaaag tagctttcga
     6541 attagcttct tatgctaaaa aagtagccga atcattagga acaacagtaa ctgccgtaac
     6601 aataaatgct tctgatgttt cagaattatc aaaatacgga gttgacaaag tattaaaagt
     6661 cactaacgac aaattggccg cttttaatgc aaaagcttat gcagatacca tcaaacaagc
     6721 tgcccaaaaa gaaggtgcaa aagtgattgt gctttcctca acaacagaca gcatttatct
     6781 tgctccatta gttgctgtag gattagaagc aggctatact tcgaatgttg ttggactacc
     6841 attaagcacc tctccttttc aagtgaaaag aaatgctttt tcaaacaaag cgttcaatac
     6901 aactgaaatt tctactgagg taaaagtgtt aggtttagct aaaaactctt atggtttagt
     6961 ggaaagcgat tcaagtttat caaccgaaga tttttcacca agtttaaatg atactgattt
     7021 taatgtaaaa gttgaaagcg ttgaaaaagt taccggaaaa gtaaccattg ctgatgctga
     7081 agtagttgtt tcaggcggtc gtggattaaa agggcctgaa aattggggaa tggtagaaga
     7141 attagcgact gttttaggag cagcaactgc ctgttcaaaa cctgtatccg atttaggttg
     7201 gagacctcat agtgaacacg ttggtcaaac cggaaaaccc gttgccacta atttatatat
     7261 tgcgattgga atctccggag caattcaaca tattgccgga atcagttcgt caaaagttaa
     7321 agtggttatc aactcagatc ctgaggcacc cttctttaaa gtggctgatt atggtgtcgt
     7381 tggagatgcc tttgaaattg ttccaaaatt gactcaaaaa ttaaaagaat ttaaagctca
     7441 aaattcataa ttttttataa attgtgccct cattgggcta tctaaatcga gatagttgta
     7501 tctttgttta gatagtccat ttttttatta ttaatttagt gtatgtacct tgtactgaat
     7561 acgttgtact gatattatat gagtcttgtt aaactagcca taaaaggaat ttcctatagt
     7621 caaacccaaa acggtgccta tgccttgatt ttaaatgagg tagatggtga gcgaaaatta
     7681 cccatcgtca ttggagcatt tgaagctcaa tctatcgcaa ttgctttaga aaaagaaata
     7741 aaaccccctc gtccacttac gcatgatttg ttcaaaaact ttgcagacag gtttgatatt
     7801 gtagtcaaac aagttatcat tcacaaatta gttgacggtg ttttctattc cagtattatt
     7861 tgcgaacgtg ataaaataga agaaatcatt gatgccagaa catccgatgc tatagcttta
     7921 gccttgcggt ttaatgcccc tattttcact tacaaaaaca tcttagacaa ggccggcatt
     7981 tacctaaaag ggaactccat ggagccagac aatccaagcg gtgaaaatga aggtgtttta
     8041 agtaatcccg aaacttttgg gttagaagaa tcaactgtaa aagatagtta tagtggtaaa
     8101 agcgttcaag aattatatga actcttaaat gaagctgttc aaaatgaaga ttatgaaaaa
     8161 gctgcaaaaa ttagagatga aatttccaaa aaagaatcat agtaaatccc cacaaaaaat
     8221 aatcctatcc taacaatcaa atgaaaatgt ataaaaattt aatttttctt tctataatct
     8281 tgctttctac tttaggttta acaggacaag aacttgaaaa aaaatggcaa ctaagttcct
     8341 caaaaacaga ttttttagaa ttgaaagaag gaggatatga attgaaaatc acctctgaca
     8401 gcattataca aaaaggggac tatcttgtac aagataatta ccttttttta tttgaaaatg
     8461 gttcggatgc tccaacaaaa agatttacaa ttcaaacaaa atctgattcg attttaactt
     8521 taaaatcaaa agacaaagct tattcatttt ttgcttctaa taaaataaaa actaccccaa
     8581 cagaagccaa attaattgcc agtcaagggt tatccgtgac cagtatttgg aaaggaattt
     8641 taggcatgct ttcactttta gctttagctt atgtttttag tgccaacaaa aaagcaataa
     8701 attggaaaac agttgggatt ggtatttttg ctcaattaat attggcaatt ggtgtgctaa
     8761 aagtgggttt tgtaaaatca ctatttgaat ttgtgggcgg attatttgtc aaagttttag
     8821 attttactag agctggaagc gagtttttat tgggtggaat gatgaatgta gataacttcg
     8881 gtttcatttt tttgtttcag gttttaccaa ctattatatt cttctctgct ttaacttctt
     8941 tattatttta tttgggaata attcaaattg ttgttaaagg tttagcaatg ttactgtcta
     9001 aactattaca catttccggt gcagagagtt tatcagtagc aggaaacatc tttttaggac
     9061 aaacggaagc tcctttgatg attaaggcct atttagaaaa aatgaataaa agcgaaattc
     9121 ttttagtcat ggtcggcgga atggccactg ttgccggtgg tgttttagct gcatatattg
     9181 gttttcttgg tggtaacgac cctcaattgc gattagtttt tgccaaacat ttattggccg
     9241 catctgtgat ggctgcacca ggagctattg tcgtttcaaa aatgttattc ccacaaacag
     9301 aatcaatcga taccaacgta gaagtttcta aagacaaaat tggctcaaac atacttgatg
     9361 ccattgctac cggaactacc gaaggattaa aattggctgc caatgttgca gccatgttat
     9421 tagtttttat tgcttttatt gcaatgatta attacggact aggcaaaata gggagtttaa
     9481 ctggtttgaa tgaaataatt gcagataaca caccttattc taaattctcc ttagaaacaa
     9541 tattaggaat tgtttttgct ccattaatgt ggttaattgg tgttgcaaaa gaagatgtga
     9601 tgttgatggg acaactactt ggaataaaac tagcggcttc agaatttgta ggatatattc
     9661 aattagccga attaaaaaac gctgcaaatg ctatccattt tacctatgat aagtcggtaa
     9721 ttatggctac ttacatgtta tgtggatttg caaattttgc ctctattgga attcaaattg
     9781 gcggaattgg ctccttagca cccggacaac gtaaaacact ttcagaattt ggcttaaaag
     9841 ctgtaattgg cggaagcata gcttctttgc tttcggctac tattgcagga atgataattg
     9901 gataattaga aagaataaca aaatgaaaca atatcatgac ttagtaaaac acgttttaga
     9961 aaacggaact caaaaaggtg atagaaccgg aaccggtact aagagtgttt ttggttatca
    10021 aatgcgtttt gatttaaatg aaggtttccc tatggtgaca accaaaaaat tacatttaaa
    10081 atcaattatt tatgaattat tatggttttt gaaaggtgat acaaacataa attatcttaa
    10141 agaaaacggc gtaaaaattt gggatgaatg ggctgatgaa aacggtgatt taggtcctgt
    10201 ttacggtcat caatggagaa attggaacag tgaagaaatc gatcaaatca gcgaattaat
    10261 cgaaacgtta aaaacaaatc caaatagccg cagaatgtta gtttctgcat ggaatccttc
    10321 tgttttgcct gacacatcca aatcgtttgc agaaaatgta gctaatggta aagcagcact
    10381 tcctccttgc catgcttttt ttcagtttta tgtagcggat ggcaaattga gttgtcaatt
    10441 atatcaacgc agtgctgata tatttttggg agttcctttc aacatagcct cttatgccct
    10501 atttacatta atgatagctc aggtctgtga tttacaaccg ggagaattta ttcacacttt
    10561 tggcgatgca catatctaca ataatcattt tgagcaagtt gaattacaac tttcccgtga
    10621 accaaaacaa ttaccaaaaa tgattttgaa tccggaaatt aaaaacattt tcgattttac
    10681 atttgatgat tttacattgg aagggtacga cccacatccg catataaaag gagttgtggc
    10741 agtttaaagc ataaaaaatc cgcttgaaaa ctcaaacgga ttaaattttt actataaaaa
    10801 caaaattaaa cggctaccac actattttct gcagcagcaa aatcattcca tttgtaagca
    10861 tctgcttctg tatcattaac aaattgacca tcaactaaat aacggaattc gtacgaattt
    10921 gaagcaggta attcaaatgt tgctttgaaa gtaccatttt ttaatttgct taattcacct
    10981 actgccggat tccaattatt gaaatctcca accacagctg ctgtagttgc gtcttttgct
    11041 tcgattgaaa aagttacttt acaaaccggt ttcgttttta taaattgttt cttaattgcc
    11101 ataactatct aatttttaga attatggtac aaagtaacaa agacaatatt gaatatcaaa
    11161 tagttaagta tatttttatc atattagcaa aaacaatagc aaaaaaaagt tatattatta
    11221 atttataaca ctttagttta cattttataa acaggaaaac ggtttcgtat ttcattatgt
    11281 gtaaatatct tttttttatt ttttaaaaaa tagtaccttt aaaaaccaaa aaacaacaat
    11341 ttcatggaaa tggataaaga actacacgaa aaatatgaat atgcccgtag aagattgaaa
    11401 caaaaaaaga gattgtattt tcattttgtt ttattcctta ttggaagttt gtttttcttg
    11461 ataattgaga aattatttga tatccaacct tttgagggtt ggtatttatg ggcagttact
    11521 atttggtttt tcttgtttat tcttcatttt attaaagtgt atattactga ccgatttatg
    11581 aataaagaat gggaaagaga acaaattaat cgattggtcg caaaacagga acaacgaatt
    11641 aaacaattag aacaaaaggc tgaagacgaa ttgaacacta gaaataatac ttctatatga
    11701 ttatcatgat tgctgctgcg gcagaaaaca atgctttggg caaagacaac gatttaatgt
    11761 ggcacttacc ggatgatttt aaacgcttta aacaacttac ttccggacat tatatcatca
    11821 tgggtagaaa aacgtttgag agctttccaa aacctttacc caatcgtact catattataa
    11881 taacccggca aaaagaatat catcccgaag gaactttagt tgttaatagt attgaaaaag
    11941 ctttagaact tattccgaaa agtcaggatg cgtttatcat tggtggaggc gaaatctata
    12001 aacaaacact tcaatttaca gataaaattg aacttacccg tgtgcatcaa tcttttgatg
    12061 ctgacactta tttcccggaa atctccctag ccagttggga actaattcaa gatgaatatc
    12121 atccaaaaga tgaaaaacat tccatagatt ttacttatca gacttacatt cgaaaataaa
    12181 aaaaaacatc ccgataaatc gggatgttca gaatcaatgt tttcatgttt tagaatcagc
    12241 ttattctagt agcaatatct gataacttaa tataaaaatt accactgcca agaaaactcc
    12301 agaaatgaag atcacgacat tggcaaacct ttgagaatac atctcaaaga agcctttaat
    12361 accaaatacc acaaaggtac taaaagcgaa aaaaactaaa atttccaaaa acaaatttaa
    12421 ccctaagaac gtttgcggca ccccaacttc aactccgctc gttagagcga aattgttgat
    12481 aatgctaaca agaagaaatg tgataactag tgccagtagt atccatttta gttcagcttt
    12541 cataactctt gataacatga tgtttaacat ttgattcatg ttgtaaaatt gcacaataac
    12601 aaatacatac acaataccca cttttagtga ttttttacat atcactaaat ttagtgatgt
    12661 attgtctaac taaaatagaa aataattaaa caatttaaat ttaatcatta ttgctatact
    12721 tacagaatag tttatatttg cctaaaattt tgagtatgtc taaaactgtc acaccctata
    12781 aaaattccaa cttaggcaaa aaagaacaag tagcacaaat gtttgatgcc atttccggaa
    12841 attatgatgg tttgaacaga gtaatatcgt ttggaacaga tataaaatgg cgtaaaaaag
    12901 ttctagccat ggtaaaatct acaaatccgg atacaatttt agacattgct actggaaccg
    12961 gtgatttagc cttgttgatg tcggaaacaa atgcaaccaa gattgtagga ttagatattt
    13021 ctgcaggaat gcttgatgtg ggccgtaaaa agattgctca aaaaaactta tccggtaaaa
    13081 tagaaatgat tttaggtgat tcagaaaaaa tgccttttga tgacaataca tttgatgcga
    13141 ttacagttgc atttggagtt cggaattttg aaactttaga aaaagggctt ttagaaattt
    13201 taagagtatt aaaaccaaat ggaatttttg tcattttaga aacttcagta ccggaaaaat
    13261 ttccatacaa acaaggttat aaattctatt ctaaaaacat tttgcctctt ataggtaaat
    13321 tattttcaaa agacaattca gcttatgctt acttatcaga gtcggcttcc gtttttccat
    13381 acggagaagc cttaaacaat attttgagaa aaattgggtt tatagacgta gtagcacttc
    13441 cgcaaacatt tggagtggca acaatttatt cagcatctaa aaagtaaaat gagaaaattt
    13501 attattctta tcatcatatt agcaggttat caaacaaagg ctcaatttaa aggaactttt
    13561 ggtaaagacc ccattattca cttagaaaat ttcgacaaac aacgcgtcca ttggggttat
    13621 tttttaggtt ttaactcgtt agatttcaaa attgatcaga attctccatc tgaagaaatt
    13681 ttagtaaata attctattgg atttaatgtg ggtttagttg gggatttacg tttatatgaa
    13741 tttttaaact tacgctttga gcccggttta tattatacac aaagagactt aactttcccc
    13801 ggatttgaag agaaagtgga ttatttaaga caagtgaaat caacttatat ctttttccct
    13861 ttgttgttaa aattttcttc agtcagaaca ggtaatgtca gaccttattt agtaggtggc
    13921 ttttcaaccg ctttgaactt atccagtaat tcgaaatcta cggatgataa ttcaaacaac
    13981 cgttttagaa tgattcaatg gacaaacttt tatgaagttg gtctgggaat tgacctatat
    14041 ttggaatatt ttaaattctc accttctatc agaggggttt tcagtttgaa cgatgaacta
    14101 attcgtgaca acgacccaaa cagcccatgg acatcttctg tagaatctat gaaaacaaga
    14161 ggaatattta tcaattttac ttttcactaa aatttcttct gaattcactt aacaaaatgg
    14221 ctgaggcagt tgccacattt aaactttccg ttttttgtaa ttgcccaaaa cgtggaatac
    14281 ttatttttga agaaattttt gcttccacta atttactaat cccatttgct tcatttccta
    14341 aaactaaaat tgccttttca ggaagagttg attcataaac gtttttacct tccataaagg
    14401 ccccataaac aggtaaaata gtgttttcta agaatttaca caaatctgta taaaccaaat
    14461 ttactcgact tatcgaaccc atggttgctt gaataacttt cggattataa acatccgcgg
    14521 tttccggtga gcaaacaagt tgttccacac caaaccaatc acacaaacga ataattgtac
    14581 ctaaattacc ggggtcgcga acatcatcca aacaaacaac taatccagtt agattaattg
    14641 gtttttgttt tggtattcta aaaaccgcta agcaatcatt aggcgttttc aagcaactga
    14701 tttttttcaa atcagattcg gctataattt caggaatagg agccgctgaa atagacaaat
    14761 caggatttgt tgtgtaaaaa tgctcggcaa tatattgcga ttggaacaat tcttgaataa
    14821 cttttatacc ctctgcaata aaaagttggt actcatttct atactttttt tgctgtaaac
    14881 tagtaattcg tttaatttga tttttactaa gcataaaaaa atgtactttt ggattaaaat
    14941 tatttcccca ttgaaaagga tttctgcaaa aatatcattt ataattttaa ttatagtact
    15001 attatacgct tgtgattcta caaaaagagt cccaaacggt gaaaatctat tggtacacac
    15061 tgaacttatt gtagatggta aaaccgttaa aaaggaagag gttatcaatt taatcggtca
    15121 aaagccaaac acacagtttt taaaattacc atttagtcta tatgtttata attgggcaag
    15181 tccgaatcct gattctcttt ttgctgaaaa attcataaag gatacccttc gatttcaaag
    15241 aacttcaagg ctattgtctg aaaaacaagt gtatcgttta ggagaatcat tttggtatag
    15301 tggcattcat aaatttttaa aaagtaatgg tgaagcccct gttttaattg atgaactcag
    15361 aactaaaaaa tcgctgtata aattaaaatc atattatttt aatgaaggct attttaaggt
    15421 aacggcaacg gaaaaaacag attctgttgg gttaaaaaaa gtgaagtcta cttataccgt
    15481 tactttagga aacccctata ttattgatag tataaccaaa aaaatagatt caccggtact
    15541 cgattcactc tatttattaa ccgaaaataa atcttccatt aaaaaaggtg aaatttacaa
    15601 ttccagtaag tttgaagagg aacgtaatcg attgacggct tatttcagaa acaatggtgt
    15661 gtataatttt cagcagaatt atatcagtta tgaagtggat accatttcaa ccggagataa
    15721 agctaatatc aatttactta ttggcaatta ttcttatcgt tcaggggata ctttagtttc
    15781 aaaacctttt gaaatattca aaatcagcga agtaaacatt tacacggata atccaaactt
    15841 aaaaagcagt tcaaaaataa cagatacaat tacctataac aattttaata tttacagttc
    15901 tgataaattt aaatacaaac ctaaagcgat aacagatgct atttttatta cgaaaggttc
    15961 cacgtatagt gatttcagaa acaatttaac atcccgttcc ttagcgaatt ttaagatatt
    16021 caattatcct tccattcttt atcaggtcaa tgaaaaagac agtctttcga acaccttaat
    16081 agctaacatt tatctaagtc cgaggaagaa atattctttc ctcccctctg ttgatttaac
    16141 ccattctgac attcaggatt ttggaattac tgcaaatctc tctgcaggaa tcaggaatgt
    16201 ttttaatggt gcagaaactt ttgatatttc gtttagagga aacattggtt cttcacgaga
    16261 caacgccaat ccaaatgatc agttttttaa tatttctgaa ataggtttag acgcaaaact
    16321 tagttttccg aggatattat ttccgcttaa cactgataat gttattccaa aaaacatgct
    16381 gccttcgaca caaatctcgg gtggatatgc taaacaaaaa aacattggat tagacaaaga
    16441 aaacttaacc ggctctttta cttataagtg gacaccgaag cgaaataatt ctattcatgt
    16501 tgatttattt tccattcagt acgttaaaaa tattaatgtc agtaattatt ttaatgttta
    16561 tcaatcgtca tatgatgcct tgaattcgat ttcaaaaaat tatgatgtta atcctgatta
    16621 ttatgatgaa aacgggacag gtgatttaat tatagaatca gggacagatg gtttcttaaa
    16681 tgatgtttta ggagacaacc caactatatt ccctacggaa gatgattaca atgaaattaa
    16741 gaacattgac ggacgtaaaa cccgtttaac agagaacaac tttatcttgt cagcaagttt
    16801 caattattct acttcaacaa aaaaaggaat attggacaat aatttctccg tatttcgaac
    16861 aaaagtagaa tctgccggaa actttctgtc gttgttttca ggagcttcta atcaaccaac
    16921 gggaccaaat ggaaataaaa caatatttga tgttgaatat tcacaataca tcaaaacaga
    16981 atttgaatat attaaacatt ttgagtttag aaaaaacagt gtaattgctt ccagagcttt
    17041 tattggtgta gccattcctt atggtaattc cgacaacatt ccgttttcac gaagttattt
    17101 tgccggaggt tctaatgaca atcgggcttg gcaaccttat agcttaggtc cgggtagcag
    17161 taatgcacaa aatgacttta atgatgccaa cttaaaaata gcctttaata ccgaataccg
    17221 atttaaacta ttcggacaag tgagaggagc tctatttgta gacgttggga acatttggaa
    17281 ctttttagac aatattgata ttgaagcagc aaagttcacg ggattgagtt cattagaaga
    17341 tattgctgtt ggttcggggt ttggaattcg gtatgatttc gggtttgtgg tggcccgttt
    17401 tgatattgga tttaaaacct acaatccggc gaatgaaatt ggtgaccgat ggtttaagga
    17461 atataatttc agtaattccg tttttaatat tggtattaat tatccattct aaaacgatat
    17521 cgtttataaa tacttaaaaa acacgacgga aaggaaacga ttttataact aataatttca
    17581 ctaaaccttt tttaattatt atttttgttg tcttaactaa tacaaattca tcatggcaca
    17641 caacataaaa ccgggcgttg ctaccggaga ccaagtacag gaaatattta gctatgctaa
    17701 agaaaaagga tttgccttac cggcagttaa tgtaattggg tcgagcaccg tgaatggtgt
    17761 tttagaaaca gctgctaaat taaatgcacc cgttatcatt cagttttcca atggtggagc
    17821 agcttacaat gccggaaaag gacttaccaa tgaaggagaa cgtgctgcta ttttaggtgc
    17881 tattgccgga gcaaaacaca ttcacacgct ggcagaagct tatggagcta ctgttatttt
    17941 acatacggat cattgtgcta agaagttact tccttggatt gacggtttgt tagatgcttc
    18001 tgaaaagcat tttgctgaaa cgggaaaacc attgttctct tcacacatga ttgatttatc
    18061 tgaagagtca ttagaagaaa atatccatat ttgtaaaggc tatttagagc gaatgagcaa
    18121 aatgggtatg acgttagaga ttgagttagg cgttacagga ggagaagaag atggtgtgga
    18181 caactctgac gttgacagtt caaaattata tacacaacct gcagaagtga gctatgctta
    18241 tgaagagttg atgaaaatca gtcctcgctt tacgattgca gcagcatttg gaaacgttca
    18301 tggggtttat aaacccggta atgtgaagtt gactccggtt attcttaaga attcacaaga
    18361 ctatgttcaa cacaaattta acacgggacc taatccgatt gattttgtgt ttcacggagg
    18421 ttcagggtca acattagaag aaatcagaga ggctatttcc tatggtgtga ttaaaatgaa
    18481 tattgatact gatttacaat tcgcttttac ggaaggaatt cgtgattata tggtgaacaa
    18541 aatagattat ttacgtacgc aaatcggtag tccggatggt gctgattcac ctaataaaaa
    18601 acattatgac ccaagaaaat gggtgcgtga aggtgaacta acgtttaaca aacgattaga
    18661 acaagccttt gcagatttaa ataatgtgaa tactttataa caaacgaact atcatttaca
    18721 gttgattata ccgctttttg attaatcaat tatccattaa aaaatacgat atggcttggt
    18781 ttaaaagaac agaaaaagga attcaaaccg caacggaaga caaaaaagat gtgccaaaag
    18841 gactttggta caaatcgcca acgggtaaaa ttattgacca ggatgaactg gctaaaaacc
    18901 tatgggtgag tcctgatgat gattttcatg tacggattgg cagtaaggaa tactttgaaa
    18961 tcttgtttga cgataatgag tttaaggagc tagacgcgaa gatgacctca aaagacccgc
    19021 ttcattttgt ggacactaag aagtattctg accggttgaa agatgcttat gagaaaacgg
    19081 ggttgaaaga tgccgttaga acagccgttg gaaaatcgaa aggaaaggat ttagtggttt
    19141 gttgtatgga ctttgctttt ataggcggtt ctatgggagc tgtcgttgga gagaagattg
    19201 cccgagggat tgactactcg ataaagaaca aagttccttt tatgatgatc tcgaaatcag
    19261 ggggagccag aatgatggaa gcggcttatt cgttaatgca gttagctaaa acttcggcta
    19321 agttggctca gttagctgag gcgaagattc cgtatatatc gttgtgtaca gacccaacta
    19381 cgggtggaac tactgcttcg tatgccatgt taggcgatgt aaacatagcc gagcccggag
    19441 ctttaattgg ttttgcgggg ccacgtgttg ttaaagatac gacaggaaaa gatttgccgg
    19501 aagggtttca aacggctgaa tttgtgttag agcacggatt tttagacttt attacgcacc
    19561 gaaaagagct gaaagacaag gttaacttgt atttagattt aatacagaac gaagacgttc
    19621 gataacagag aatcccgagt aatcgggatt ttttgttttt aggaggtatt tattttagcg
    19681 ggggctgttt tatttgtgat ggatggtatg tggatggaat ggttgtggat tatctatctt
    19741 aatttaatta aagttactat atagttacta taatgttact atatacttac tataaagtta
    19801 ctatatttta cttttaaact tattttattg ttattttaaa taatttgtat atctttggtt
    19861 ttcattaggt tatcaacttt taaaaaaaga tacattatgg caaggcaaaa aggtattatt
    19921 aagcttgagg gcacattgga tgggttgaat ttttataatc gattggggga gccgttgtcg
    19981 cggattgcgg gtggtggttt taattcggtg gcgataaaga ctaagccgag catgtctcgg
    20041 gtgcgggaga atggcagtga gtttaagact tgtatgactt cagtgaagta ttttaagctt
    20101 ggtttgcaac cgattagtta tcaatttaag gatacgttgt tgcatcaacg gttagtgcag
    20161 ttgtttaccc gcatcaaggt ttttgattcg gtttcagagc gtggacaacg aacggtgggg
    20221 attggtttgc agagtgctgc ggggaagaag ttattgcatc gttatgtgtt gacttcgggt
    20281 gacggcttgg attcggtttt acggcaggga tttgaggcgg actggcaatt gaacggtttt
    20341 aggattgcgg catttgatat ggcagctgtt cgatttccga agggagcgac gcatgtgtcg
    20401 ttgcaggccg ggtatttggt ttttgacttt acgacgttta cgccagtttt agcgaagagt
    20461 gctccggtgg tgctttcgaa ggctacggca acggctccgt tggagttggg cgttagtgct
    20521 ttgccagttg gtgcgggaac gaaagttatg gtggtttatt tgcagtttat gcaggaggtg
    20581 aatggtttta tgtatccgat gaaggagttg aacacccgtg tttttgaggt ggtttatgtg
    20641 gattaattga tttttttagt ttttttattt catcaagctc agtataaatt ttttttagct
    20701 tttttttaga ttctatagtt tttgattctt ttaaattatt ttatgtaaac ctattttagg
    20761 acaagtcatt atttagatga ttgaactagg gtgttttatt ggtgtgttta attacgatgg
    20821 aaaagcgttt ggttttgctg agaagtttaa gcagcttgcg gagggagatt ttagtaacca
    20881 cacgaatgga tttgtgcgga tgtaggtgaa aagagagcac aacaacaagt agttaaagat
    20941 aaaaaggaac taaaagaaat aattaattca ctattgcaac aataaatatg acaaaaaaca
    21001 ttttattttc aatatgcata atattttgta tttcatgcag aaaagaggtt gaagtaactc
    21061 aaaaatcagc ttatcaagac ataaaattta tttttcctga tacagtttat gttaatgaaa
    21121 gttatgatgg gcaaattagt tttaaaagtg attttgatac aattacaaca agtttaagtg
    21181 attatcaaaa agcaaggttt cttgagtatt ctttttggtt gacaaaaaaa ccaattgaag
    21241 acaacaaact tttaaaggaa atggttacag atacttttgt agcggaaaca aatagagtaa
    21301 tccctttata tgatataaag tttgataagc tgggagtaaa ttattttaat ggtattataa
    21361 aagatgaaat tatcatagcc aaaggcggta ttctgaaaga tggaagtaaa ggagatagaa
    21421 taataaccaa tgaaataagg cttacacgtg ctgtatatgt aattgataat agggctgaca
    21481 catcccgctc cgcattctat gataaaaact agtaaaataa atccattttt tttataatat
    21541 atttagtaga agatgatttg aagcaaagaa ttttcattgg aaaagcgttt ggtttttctg
    21601 caaagtctat ag
//
LOCUS       DAOTCW010000025        21504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1300_length_21504_cov_47.257728, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000025 DAOTCW010000000
VERSION     DAOTCW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1300_length_21504_cov_47.257728"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /gene="ruvC"
                     /locus_tag="P5335_03595"
     CDS             complement(<1..553)
                     /gene="ruvC"
                     /locus_tag="P5335_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRZ73997.1"
                     /translation="MANERIILGIDPGTTIMGFGLIKVINKKMEFIQLNELILSKYDD
                     HYTKLKVIFERTIELIETHHPDEIAIEAPFFGKNVQSMLKLGRAQGVAMAAGLSRQIP
                     ITEYEPKKIKMAITGNGNASKEQVAKMLQQLLGLKELPKNLDSTDGLAAAVCHFFNSG
                     KVVGEKSYTGWDAFVKNNESRVKK"
     gene            648..1571
                     /locus_tag="P5335_03600"
     CDS             648..1571
                     /locus_tag="P5335_03600"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HRZ73998.1"
                     /translation="MVLAKLLLVGGALYYLFQQLQQKSISNFDGIVSNFSAFSLLGII
                     LLSVLNWLFEFLKWKNLVSSFHKITFKQAVEQTLGSLTASIFTPNRVGEYVAKCLYYS
                     KEKTKRIIFLNFLGNITQMMVTVFFGVFGVLFYFIFSPFSFDIEYNANEKGLIFWLPI
                     LLVILLSVLLFYYKKINFYGYSVEKIMVKMQLFPARKHFVNVQYSLLRYIIFSHQFYF
                     LLLIFQVDIPYSDAILTIFTMYFLASVIPSIHLMDVVIKGSVALFLFGKLGVNEWVIA
                     SITIIMWFFNLVIPVLLGSFFVLKFKPKMDL"
     gene            1571..>1878
                     /locus_tag="P5335_03605"
     CDS             1571..>1878
                     /locus_tag="P5335_03605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRZ73999.1"
                     /translation="MVFIYVSITIYVILLFLFCVAFKKVKRFSPSSKELKHSFSIVVP
                     FRNEAHNLPQLLKSIEQLNYPIEKFELILVDDESTDGYQVSSYGFRVVELKNIRNSNS
                     P"
     assembly_gap    1879..1978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1979..2188
                     /locus_tag="P5335_03610"
     CDS             <1979..2188
                     /locus_tag="P5335_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRZ74000.1"
                     /translation="PFLSIGYFKLIPIFILKILVDLFLANQTAAFYQIRMKNRFLTAL
                     FYPFFSSAVALYSLFGKYKWKDRKV"
     gene            2269..3033
                     /locus_tag="P5335_03615"
     CDS             2269..3033
                     /locus_tag="P5335_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRZ74001.1"
                     /translation="MLYSKIEGKGKPLIILHGFLGMSDNWKTLGTQFAANGFQVHMLD
                     LRNHGRSFHSEEFNYKVMMSDVAEYCRFHQLTKIDIIGHSMGGKVAMLLAVEHPEMVE
                     KLVVADIGPKYYPSHHQDILAGLNAVDFSQKPDRNQVEEVLQKYIPDFGTRQFLMKSL
                     FWKEPGQLAFRFNLEVFNKEIEQIGTALTDSQRFDKPTLFIRGGNSKYILEGDFQTIQ
                     YHFSNSKIETIPNVGHWLHAENPKLFFEIVIGFLNT"
     gene            3067..3411
                     /locus_tag="P5335_03620"
     CDS             3067..3411
                     /locus_tag="P5335_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRZ74002.1"
                     /translation="MNLLIRILITAGLVMLIANFLPGVKVDSFVQSIYVAIILGLLNL
                     FIKPIIILFTLPVTILTLGLFLLVINAIIVLLCSNIVGGFHVESFWTALFFSIILSVL
                     QSIVYKLTGGEE"
     gene            3513..4838
                     /gene="tig"
                     /locus_tag="P5335_03625"
     CDS             3513..4838
                     /gene="tig"
                     /locus_tag="P5335_03625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRZ74003.1"
                     /translation="MNITRTNVDALNAVVTVAVSKNDYASKVEKVLADYRKNASIPGF
                     RKGAVPMSLIHKQYGKAVLLEEVNKVLQENLNKYLVDEKLDILGNPLPKVTEDFDWDK
                     EDFSFDFELGLAPEFSVDLAAKNNITSFKIIADDKMLNGQVERIQKQYGKLISKDTVE
                     EDDDLNVNFTNEANGINNTTSISLDVFKDKKAAKKFIGKKVGESVELSTKGLFDDDHK
                     LMDYLKVDHDLVHGLDIEVSATIEGITTSEKAELNQELFDKLFGEGNVTSVDGVKAKI
                     KEDAEKQFVQQADQKFLNDVTEFLLESTKFDLPAEFLIKWLQHAGEKHLTEAEAREEF
                     LKSEKGLRYQLIEGKIMTENNLQITFEDLKAFTSELIKKQMAQFGQMNPTQEDIDGIV
                     ARVLSNQDEVKRLSEQVMNEKMLGLFKEKVKAKTKEVSYEQFVKEMYGE"
     gene            4931..5590
                     /gene="clpP"
                     /locus_tag="P5335_03630"
     CDS             4931..5590
                     /gene="clpP"
                     /locus_tag="P5335_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRZ74004.1"
                     /translation="MNIGKEFKKFATKHHGVNSMYYDKIVSGMTPYIIEERQMNISQL
                     DVFSRLMMDRIIFLGTGVDDYVANIIQAQLLFLESVDASKDVQIYINSPGGSVYAGLG
                     IYDTMQYIKPDVATICTGMAASMGAVLLCAGAAGKRSALPHSRVMIHQPSGGAQGVAT
                     DMEINLREMLKLKDELYQIISTHSGQTFEKVHKDSERDYWMIADEAREYGMIDEVLRR
                     K"
     gene            5656..6891
                     /gene="clpX"
                     /locus_tag="P5335_03635"
     CDS             5656..6891
                     /gene="clpX"
                     /locus_tag="P5335_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRZ74005.1"
                     /translation="MAKEMMECSFCGRKKPETNLLIAGIDAHICDKCIEQAHGIVLEE
                     LKQSKHSQSLGNDLVLKKPKEIREFLDQYVIGQDQTKKVMSVAVYNHYKRLMQKNSDD
                     DVEIEKSNIIMVGQTGTGKTLVAKTIARMLGVPLTIVDATVLTEAGYVGEDVESILTR
                     LLQVADYDVAKAERGIVFIDEIDKIARKSDNPSITRDVSGEGVQQALLKLLEGTVVNV
                     PPKGGRKHPDQKFIEVNTQNILFIAGGAFDGIERIISKRLNRQAVGYSASAKVDNVDK
                     DNLMQYIIPKDIKDFGLIPEIIGRLPVLSYMNPLDRETLRTILTEPKNALIKQYKKLF
                     EMDGVEFSISEEALDYIVDKALEYKLGARGLRSLCEAILTDAMYDLPSSDEKILHIEK
                     SYAEHTLNKNMLQRLKAVS"
     gene            complement(6908..7072)
                     /locus_tag="P5335_03640"
     CDS             complement(6908..7072)
                     /locus_tag="P5335_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74006.1"
                     /translation="MKKITLFLICIFLYSCGPKRLGCGPGRRCEINSKKENFQLIPQK
                     NPEVKTSGLV"
     gene            complement(7074..8600)
                     /locus_tag="P5335_03645"
     CDS             complement(7074..8600)
                     /locus_tag="P5335_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRZ74007.1"
                     /translation="MPLPIINSIASWILKKRIHQIELFMKYPNEVQEELLFSLLKTAE
                     NTVVGRQYDFNSITHYSVFSERIPISSYEDLEPMIEQTRKGMQNIFWPTSIKWFAKSS
                     GTTNAKSKFIPVSNEALENCHYKASKDLLSLYLNNNEDSQLFVGKSLRLGGSKELYED
                     NNTFFGDLSAILIDNMPIWAEFSSTPSNRVSLMGDWEIKLMAIVQETIYEDVTSLAGV
                     PSWMMVLLNKVLEETGKTNLLEIWPNAEVYFHGGVSFDPYREQYKKLFPRSDFNYYEI
                     YNASEGFFAIQDLNNSNELLLMLDYGIFYEFIPMETFGTINQKVIRLADVELFKNYAV
                     VITTNSGLWRYLIGDTIRFTSLNPHRIRVTGRTKHFINVFGEELIVENTDKAIAKACL
                     ETESEVVDYTVAPVFMEGKKKGAHEWIIEFRKPPENIGYFRKIVDDTLQALNSDYEAK
                     RRNNMTLNPLIINVARPQLFYDWLKEKDKLGGQHKIPRLSNERTYLEQLLQMQYVAQS
                     "
     gene            8729..9523
                     /locus_tag="P5335_03650"
     CDS             8729..9523
                     /locus_tag="P5335_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HRZ74008.1"
                     /translation="MIYEGVLTKMQTELGNPIQYYLVFENSFLNLNQLLDKELEISFQ
                     GYQCLQCGKKKKIFRQGYCYDCYMSSASVGDWIMKPELSTAHLDIEDRDLDYERKVQL
                     QPHIVYLALSSEVKVGVTRGTQVPTRWIDQGAVQAIPILELPNRYLAGITEVALKNNY
                     ADKTNWQKMLKNEVPQVDLIQERLKAGNFLPDEVRDYYAENNSHLYEFHYPVLTYPKK
                     VTSLSLDKTPLFQGKLSGIKGQYLLFQDGTVFNVRTFEGYVVKISL"
     gene            complement(9554..12142)
                     /locus_tag="P5335_03655"
     CDS             complement(9554..12142)
                     /locus_tag="P5335_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRZ74009.1"
                     /translation="MFKKILKGLGVFLAIVIIALAITPFLFKDKIKELVLKSINEKVD
                     ATVAFEDVSLSLFKNFPKANITIDKLSIINKEPFAGDTLFYSGELNLKMSVKELFKAD
                     GEAMELESFSSNNGLVNIIFNKDGIGNFDIALKDQKDDTSSKSEPFKLNIQNYSISNL
                     KFKYLDEASTMNVIINDIQHNGKGNFAASTLDLVTTSSGNLSVDFDKINYMNKVAITL
                     DAVLGIDLEKSKYEFKNNKALVNQLPLEFKGFIQLVENGQLYDLTFHTPSSSFKNFLG
                     LIPSAYKGDLDKIKTSGDFVVSGFAKGFYTDSTIPKFELVIASTNASIKYPDLPKSVE
                     NIIIDTKIINETGLMNDTYVAIDNLSFKIDQDIFNAKASIKNITENALIDATLKGTIN
                     LSNLSQAYPIKVDKPLSGILKADVTTKFDMQSVEKEEYQNIKNAGLISVSGFKYIDEN
                     GKGMDINEAVVQFNPSKLNLQKFEAKTGKSDMNVTGFLDNFYGYLFNKQNLKGNFNLI
                     SNQLAVADFMTTSETENKGKKEIEAMKIPAFLDCTLTAKATTVLYDNLILKDVSGKLI
                     VKDEKVTLENIKTSIFGGQIGLNGNVSTKTKVPTFKMDLNLNQVNISETFTQLDMMKN
                     IAPIANVINGKLNSTITVSGNLDAKEMTPDLKSISGDLMGQLLSTTINASNSMLLNAL
                     DNQVSFIDLKKLNLNDLKTSLSFKDGKVNVKPFDIKYQDITVNVGGTHGFDQSMNYNV
                     KFDVPTKYLGPEINNLIAKLSPADAAKLQNIPITALMSGNFTNPKITTDIKQATTKLA
                     NQLVEQQKQKLINQGTSALTNLINQNTKPTDTTKTKTPVTKEQEIKDNLKEGLNSLLK
                     PKKKDN"
     gene            complement(12212..12901)
                     /locus_tag="P5335_03660"
     CDS             complement(12212..12901)
                     /locus_tag="P5335_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HRZ74010.1"
                     /translation="METKDKLFAYKIYFALGALFIASLVVSNLIFQKFFYWDFFGIYR
                     FEISVGILPYPLTFLITDIISEIYGRKKANQVVTSGIFASVFSLMIVYVANAVPAIES
                     SPVNNAMFSKVFGQTALAVLASMIAYLTAQYIDIYIFHFWKKVTKGKMLWLRNNFSTF
                     TSQFVDTFAVLFLLCSFRILPWDLFPKLMLSGFLFKVIFALLDTPFLYAAVYLFQKRF
                     NLKRGEELVLD"
     gene            complement(13021..>13374)
                     /locus_tag="P5335_03665"
     CDS             complement(13021..>13374)
                     /locus_tag="P5335_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HRZ74011.1"
                     /translation="RKMTVEQVEAVAQGRVWTGSEALKLGLVDQLGGLDLALKEAATL
                     AKIKKFKTQNYPEFDKSIMDFINDQNKLPFAKSKETIIKEEIGVEAYETLKQVKKLTA
                     HKGIQMLMPFELKIQ"
     assembly_gap    13375..13474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13475..14373)
                     /locus_tag="P5335_03670"
     CDS             complement(<13475..14373)
                     /locus_tag="P5335_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HRZ74012.1"
                     /translation="MKFLGNVLATIIGLFVFCMILFFGLLIFSAIAGSDSDVAKVKSD
                     SVIELDMENIALDYTGKFKDPWVTLLSDETSVGLIDVLNAIEEAKIDDKIKGISIVNS
                     TSNLGLAQLKALRNKLNEFKKSGKFIVSYADFFTQKEYYINSIADTIYMNPVGEMDFK
                     GLSTEVMFFKDIQEKTGVKMEVIRHGKYKSAVEPFLENEMSEANREQISALINSAWSS
                     IVSDISLSRNIRVDSLNNIANQLSARTPEMALSKKLIDKIGYEDQYHNGIRKALKVDK
                     DEDYNSVSIMDYAKNVAKSPQKV"
     gene            14534..15667
                     /gene="folK"
                     /locus_tag="P5335_03675"
     CDS             14534..15667
                     /gene="folK"
                     /locus_tag="P5335_03675"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRZ74013.1"
                     /translation="MNSQNQVILSLGSNLGNRLENIQLAIDAIHNSIATVVKVSSLYE
                     TPAWGFESNSFYNCALVLQTLKPAPQILDEILILEIKLGRVRNDAEGYQARVIDIDVI
                     TYNEEIVETEKLQVPHPQMQNRLFVLLPMRDLNLDWRHPILQKYLPELVQLSEDKSNC
                     KVVQKLKSPLEKIDLQQYNYIAIEGNIGAGKTTLATKISEDFNAKLVLERFADNPFLP
                     KFYEDQSRYAFPLEMSFLADRYQQLSDDLAQFDLFKDFIVTDYHIFKSLIFAKVTLAE
                     DEYRLYKTMFDIIHKEMPKPDLYVYLYQNTEQLLKNIKSRGRRYEQQIPAEYLDKINR
                     GYLDYIKTQTDLKVLIIDVSNKDFVENQIDYVSILDEIQQKIQ"
     gene            complement(15669..>16172)
                     /locus_tag="P5335_03680"
     CDS             complement(15669..>16172)
                     /locus_tag="P5335_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HRZ74014.1"
                     /translation="ENCSNTKHFFITKHTAPIIEEVLVTNQTATILISGIGEFEFSID
                     GYSYQDSNVFAVEEGGLYTAYVREKYDCGEDNDQFVVINIPKFFTPNNDGFNDVLTIK
                     GIEYFPNASLTIFDRYGKFIKKIDQTNRDWDGTLNGKKLPSNDYWFILKTGDGRPDTK
                     GHFSIIR"
     assembly_gap    16173..16272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16273..17890)
                     /locus_tag="P5335_03685"
     CDS             complement(<16273..17890)
                     /locus_tag="P5335_03685"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HRZ74015.1"
                     /translation="MKIRLLYLVLFSLPFFSYSQNVAPILTATGNQVYCPGTQLRIVT
                     DMTIVDPDDTGIDAIYIQISSGYVSGQDLLSLTGNHPTLTSSWNTLTGKLTLAGVSTQ
                     PTYTNLIAAIKDVVFSNSSANPSGIRTFSITVGQANYLPSNGHYYQYVPNIGITWSSA
                     KSAAQASTYYGLQGYLATITAADEAQLSGEQAAGAGWIGGSDEQTEGVWRWMTGPEIG
                     TIFWNGGVNGFSPNFAFWNSGEPNNLGDENYAHVTAPGVGISGSWNDLSNTGESNGNY
                     QPKGYIVEYGGMPGDPILQISTSSTIVIPSITSTAAQSRCGDGTINLSATASNGIVKW
                     YANSTGGTPIATGNTFTTPVLSSTTSYYVDAFESNCTTATRAVVTATIIPIPIVTVTP
                     PATVCGNNSTVLVASTSAGTISWFDTAFGGTSLATGTNFTTPNLTQNTTYYVEATNNG
                     CSSGNRLPVVVTVFDIPDVSDENKIHCEGIPLTLDAGILNVSYLWSTGETTQTIQITD
                     DSDYSVIVTSLPPENCSNTKHFFITKHTAPI"
     gene            complement(17907..18443)
                     /locus_tag="P5335_03690"
     CDS             complement(17907..18443)
                     /locus_tag="P5335_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRZ74016.1"
                     /translation="MRKLANSELDRKNINEFKDAKKTPIIIILDDIRSLHNIGSVFRT
                     SDAFLIEKIYLCGITATPPNKEIHKTALGATDTVEWEYQENVLTVIDRLKSENVQIFA
                     IEQVENSIMLNDFQIEKEKKYALIFGNEVKGVSQEAIHESDGVIEIPQLGTKHSLNIS
                     VSAGIVVWDLFQKIKVSK"
     gene            18602..21169
                     /gene="mutS"
                     /locus_tag="P5335_03695"
     CDS             18602..21169
                     /gene="mutS"
                     /locus_tag="P5335_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044047604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRZ74017.1"
                     /translation="MKQYNEIKRKYPDACLLFRVGDFYETFGEDAVRASQILGIVLTK
                     RGAGSETETALAGFPHHSLNTYLPKLVKAGLRVAICDQLEDPKMTKTIVKRGVTELVT
                     PGVSMNDEVLQSKSNNFLASVYFGKKQIGISFLDVSTGEFLTAQGNEEYIDKLLQNFI
                     PSEILVTKNHKQHFREVFGEDYHTFFLEDWVYNEDYGREVLAKHFQTNSLKGFGIEDL
                     QEGIVASGAVLYYLSETQHNKIQHITSIQRIAEDAYVWMDRFTIRNLELYHSTNPNAV
                     TLLDVIDKTLSPMGSRLLKRWLALPLKDIIKIQSRHEVVSFLKENSDVLRQIQYQIKQ
                     ISDLERLISKVATGKVSPREVMNLCYSLDAIIPIKTLALASSNESLRAIGDSLHSCDL
                     LREKIKTTLNPDAPVSINKGNAVAKGINAELDELRAISTSGKEYLEGIESRESQRTGI
                     SSLKISFNNVFGYYIEVRNTHKDKVPPEWIRKQTLVNAERYITEELKEYETKILGAED
                     KIHLIETQLFEQLVIWIGTYIKPVQLNAGLIAQLDCLNSFTQLAIDNKYVRPILDDSY
                     ELEITNGRHPVIEKQLPVGVPYIANDVFLDRDSQQIIMITGPNMSGKSAILRQTALIV
                     LLAQMGSFVPAEAVRMGIVDKIFTRVGASDNISMGESTFMVEMNETASILNNISERSL
                     VLLDEIGRGTSTYDGISIAWAIAEYLHEHPNRPKTLFATHYHELNEMEVTFERVHNFN
                     VSVKELKDTVLFIRKLVKGGTAHSFGIHVAKMAGMPQTVIQKAQKILKKLEKNHSSEE
                     LSGIKSSKDELQLSFFNLDDPLLEEIKEEILNIDINTLTPVEALMKLNEIKRMLIKK"
     gene            21308..21380
                     /locus_tag="P5335_03700"
     tRNA            21308..21380
                     /locus_tag="P5335_03700"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21341..21343,aa:Gly,seq:gcc)
     gene            21419..21504
                     /locus_tag="P5335_03705"
     tRNA            21419..21504
                     /locus_tag="P5335_03705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21453..21455,aa:Leu,seq:taa)
BASE COUNT         6955 a         3290 c         3690 g         7269 t
ORIGIN      
        1 attttttcac acgactctca ttattcttca caaaagcatc ccatcccgta taacttttct
       61 ctccaactac tttccctgaa ttaaaaaaat gacaaaccgc cgccgccagt ccatccgtag
      121 aatctaagtt ttttggtaat tcttttaaac ccaacaattg ttgtagcatt ttagctactt
      181 gttctttact ggcgtttccg tttccggtaa tggccatttt tatctttttg ggttcatatt
      241 cggtaattgg aatttgtctc gacaatcccg ctgccatcgc cacgccttga gctcgtccga
      301 gtttgagcat cgattgtaca tttttcccaa aaaacggagc ttcaatggca atttcgtccg
      361 gatgatgggt ttcaatcaac tcaatcgtgc gttcaaaaat cacttttaat ttggtataat
      421 gatcatcata tttactcaaa atcaattcat ttaactgaat gaattccatt tttttgttaa
      481 tgactttaat taaaccaaat cccataatgg tggttccggg gtcgatgcct aagatgatgc
      541 gttcgtttgc caaaattaga gtttaaaagt ttactattta aaagtgtaaa gttttgcttt
      601 actttgtact gatggttaca ttatctcaca aagctaaaca attctcattg gttctcgcaa
      661 aacttttgct tgtgggcgga gcactttatt atttgttcca acaattgcaa caaaaaagca
      721 tttcaaattt tgatggaatt gtgtctaatt tttcagcttt cagtttgctg ggtattatat
      781 tattaagtgt tttgaattgg ttatttgaat ttttgaaatg gaaaaattta gtttccagtt
      841 tccataaaat aactttcaaa caagccgttg aacaaacatt ggggtcactg acggcttcta
      901 tttttacgcc taatcgggtt ggggaatatg tggctaaatg tttgtattat tcaaaagaaa
      961 aaacgaagcg aataattttt ttaaattttt taggcaacat tactcagatg atggtaactg
     1021 ttttttttgg agtttttggt gtgctttttt attttatttt ctctcctttt tcctttgata
     1081 tagaatacaa cgcaaatgaa aagggtttga ttttctggct tccaattctt ttagttatac
     1141 ttctctctgt tttgcttttt tattataaaa aaatcaattt ttatggttat tcagtggaga
     1201 aaataatggt taaaatgcaa ttatttcctg ctcgaaaaca ctttgtaaat gttcaatatt
     1261 cgttacttag gtatattatt ttttcgcatc aattttactt tttgttatta atttttcaag
     1321 ttgatattcc ttattcagat gctattctta ccatttttac catgtatttt ttagcttctg
     1381 ttataccaag tattcatttg atggatgtag ttataaaagg tagtgtagcg ttatttttat
     1441 ttgggaaatt aggtgttaat gaatgggtaa ttgcttcgat tacgattatt atgtggttct
     1501 ttaatttagt tattccggta ctattaggga gtttttttgt gttgaagttt aaaccaaaaa
     1561 tggatttgta atggtgttta tttatgtatc tattacaatt tatgtgattt tattattcct
     1621 attttgtgta gcatttaaaa aagtaaagcg attttcacct tcttcaaaag aacttaaaca
     1681 ttctttttca attgttgttc cttttcgaaa tgaagctcat aatttgccgc aacttttaaa
     1741 atcaattgaa caattaaatt atccgattga aaaatttgaa ttgattttag ttgatgatga
     1801 atctacggat ggttatcaag tttcgagtta cgggtttcga gttgttgaat tgaaaaacat
     1861 acgaaattca aattctccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     1981 tttccttagt attggttatt ttaaactgat accaattttt attttgaaaa ttttggttga
     2041 tttgtttttg gctaatcaaa cggcagcatt ttatcaaata agaatgaaaa ataggttttt
     2101 aacagcattg ttttatcctt tttttagctc agcagttgct ctttattctt tgtttggaaa
     2161 atacaaatgg aaagaccgaa aggtataacc ggaacttgtg gttagcaaat gaaataagta
     2221 acttgcgaca aattattaca attgactttg cttttaaaat caaaatttat gttgtattca
     2281 aaaatagaag gtaaaggtaa accactcata attctgcatg gatttctcgg gatgtctgat
     2341 aattggaaaa ccctcggaac tcaatttgcg gcaaatggtt ttcaagttca tatgcttgat
     2401 ttaagaaatc acggacgaag ttttcattca gaggaattta attataaggt catgatgagt
     2461 gatgtggcag aatattgtcg atttcaccaa ttgacaaaga ttgatattat tggtcattcc
     2521 atgggcggta aagtagctat gcttttagcg gttgaacatc ctgaaatggt tgaaaagttg
     2581 gttgttgcgg atattggccc aaaatattat ccgtctcatc atcaggatat tttggcaggt
     2641 ttgaacgctg tagatttttc acaaaaaccg gatcgtaacc aagtggagga ggtcttacaa
     2701 aaatatattc ccgattttgg cacccgccaa tttttaatga aaagtttgtt ttggaaagaa
     2761 ccgggtcaat tggctttccg atttaatttg gaggttttta ataaagaaat tgaacaaatc
     2821 gggacagcat taacagatag tcaacgattt gataagccta ctttgtttat aagaggcgga
     2881 aattcgaagt acattttgga gggagatttt caaactatac agtatcattt ttccaattca
     2941 aaaattgaaa ctattccaaa tgttggacat tggctgcatg ccgagaatcc taaactattt
     3001 tttgaaattg taattggttt tttgaataca taatattttt ttgtattttt attaaaaata
     3061 aaccttatga atttacttat tcgaatttta atcacagccg gtttagttat gttaattgct
     3121 aattttcttc cgggtgtaaa agtggacagt tttgttcaat cgatttatgt ggccattatt
     3181 ttaggattac ttaatttgtt tatcaaacca attattatct tgtttacttt gccggtaacg
     3241 atactaacgt tagggttgtt tttattggta attaatgcaa ttattgtcct tttgtgcagc
     3301 aatattgtag gtggttttca tgttgaatca ttttggacgg ctttgttttt tagtatcatc
     3361 ttatctgttt tgcaatctat tgtttataaa ctaacaggcg gggaagagta attttacggc
     3421 tgatgaacta aaatacaatc atttaaaaac ttggttgggt tgcaaaaaag ttatactttt
     3481 gcaacccaat tttagtatat aaatcacaag aaatgaatat tacaagaacc aatgtggatg
     3541 ctttgaatgc ggtggttacc gtagcggttt ccaaaaatga ttatgcttca aaagtagaaa
     3601 aagtattagc tgattatcgc aaaaatgctt ctattccggg ttttagaaaa ggagctgtgc
     3661 cgatgagttt aattcataaa caatacggaa aagcagtatt gttagaagag gtaaataaag
     3721 ttttgcaaga aaatttaaat aaatatttag tagatgaaaa attagatatt ttaggaaatc
     3781 cacttccaaa agtaacggaa gatttcgatt gggataaaga agatttttct tttgatttcg
     3841 aattgggatt agctcctgag ttttcagttg atttagctgc aaaaaataac attaccagct
     3901 tcaagattat tgctgacgat aaaatgttaa atggccaagt ggaaagaatc caaaagcaat
     3961 atggaaaatt aatttctaaa gatacggttg aagaagatga tgacttgaat gttaacttta
     4021 caaatgaagc taacgggatc aataatacaa cgtctatttc gttagatgtt tttaaagata
     4081 aaaaagctgc aaaaaaattc attggaaaaa aagttggtga aagtgtagaa ttaagtacca
     4141 aaggtttatt tgatgatgac cataaattga tggattatct taaagttgat catgatttag
     4201 tgcatggttt agatattgaa gtttctgcta caattgaagg aattacaaca agtgaaaaag
     4261 ctgaattgaa tcaagaatta ttcgataaat tatttggtga aggcaacgta acttcagtcg
     4321 atggagtgaa agctaaaatc aaagaagacg ctgaaaaaca atttgttcaa caagcagatc
     4381 aaaagttttt aaatgacgtg actgagtttt tgttagaaag cacaaaattt gacttaccgg
     4441 ctgaattttt aattaaatgg ttgcaacatg cgggcgaaaa acacttaacg gaagcagaag
     4501 ccagagaaga gtttttaaaa tcagaaaaag gccttcgtta ccaattgatt gaaggaaaaa
     4561 taatgacgga aaacaattta caaattactt ttgaagattt gaaagcgttc acctctgaat
     4621 taattaagaa acaaatggct caatttggtc aaatgaatcc gactcaggaa gatattgatg
     4681 gcattgtagc tcgagtactt tctaaccaag atgaagtgaa aagactgtct gagcaagtga
     4741 tgaatgaaaa aatgcttggg ctgtttaaag agaaagttaa agctaaaaca aaggaggttt
     4801 cttacgaaca gttcgttaaa gaaatgtacg gagaataata atttcaaaaa aagtagtata
     4861 tttgggcgtc taatgtatat ttgacgctct tttacattta aaaatgtgca cttaaaattg
     4921 tttttttaaa atgaatatag gaaaagaatt taaaaaattt gctactaaac accatggtgt
     4981 aaatagtatg tattatgata aaattgtaag cggaatgacg ccttacatca tcgaagaacg
     5041 tcagatgaat atttctcaac ttgacgtttt ttcgcgtttg atgatggaca gaattatctt
     5101 tttgggaaca ggagttgatg attatgtagc caatattatt caagcacaat tgttgttttt
     5161 agaaagtgta gatgcctcta aagacgttca aatttacatt aactctccgg gaggaagcgt
     5221 ttatgcaggg ttgggaattt atgatacaat gcaatatata aaacctgacg tagcgacaat
     5281 ttgtaccggt atggctgctt caatgggagc tgttttatta tgtgccggag cagcaggaaa
     5341 acgttctgct ttgccacatt caagagtgat gattcaccaa ccttctggag gagcacaagg
     5401 tgttgcaaca gatatggaaa tcaatcttcg tgaaatgttg aaattgaaag atgaattgta
     5461 tcaaattatc tctactcatt ccggtcagac ttttgaaaaa gttcacaaag acagtgaacg
     5521 cgattattgg atgattgctg acgaagccag agaatatggt atgattgacg aagtattaag
     5581 aagaaaatag ttttggtaat ttgtatggct cataagccaa attaacaatt acttataacc
     5641 ttttacccaa ataaaatggc aaaagaaatg atggaatgtt ctttctgtgg aagaaagaaa
     5701 cccgaaacga atttgttaat cgccggaatt gatgcacaca tttgtgataa atgcattgag
     5761 caagcacatg gtattgtttt agaagaactg aaacaaagta aacactctca gagtctcggc
     5821 aatgatttgg tattaaaaaa acccaaagaa attcgggaat ttttagatca gtatgtcatt
     5881 gggcaggacc aaaccaaaaa agtgatgtcg gtagcggttt acaatcatta caaacggtta
     5941 atgcagaaaa atagtgatga tgacgttgaa attgaaaaaa gcaacatcat aatggtaggt
     6001 caaaccggaa caggaaaaac attagtagct aaaacaattg ctcgaatgtt aggtgttccg
     6061 cttacaattg tagatgcaac cgttttgaca gaagccggat atgtgggtga agatgtagaa
     6121 agtattttga cccgattact tcaggtagct gattatgatg tggctaaagc agaaagaggt
     6181 attgttttca ttgatgaaat tgataaaatt gcccgtaaaa gtgataatcc atctattacg
     6241 cgtgatgttt ccggagaagg tgttcaacag gctttgttga aattattaga aggaacagtt
     6301 gtgaatgttc caccaaaagg tggcagaaag catcccgatc aaaaatttat tgaagtaaat
     6361 acacaaaaca tattgtttat tgccggtgga gcttttgatg gaatcgaaag aataatttcc
     6421 aaaagattaa atcgtcaggc agtaggctat agtgcttcag ctaaagttga caatgtagat
     6481 aaagataatt taatgcaata cattattcca aaagacatta aagattttgg attaattcct
     6541 gaaattattg ggcgtttacc ggtattgtct tatatgaatc ctttagatag agaaacactt
     6601 cgaactatct tgacagaacc aaaaaatgct ttaatcaaac aatataaaaa actgtttgag
     6661 atggatggtg ttgaattttc aatttctgaa gaagccttag attatattgt cgacaaggcg
     6721 ttagaataca aattaggagc tcgtggatta cgttcacttt gtgaagctat attaacggat
     6781 gctatgtatg atttgcctag ttcagacgaa aaaatattgc acatcgaaaa aagttatgct
     6841 gaacatacgt taaataaaaa tatgttacaa cgtttgaaag cagtatctta aaaagtagat
     6901 agaaaaatta tactaatcct gaagttttta cttcaggatt tttttgtgga ataagttgaa
     6961 aattttcttt tttggagttt atttcacatc ttcttcccgg tccgcaacct aaacgttttg
     7021 gtccacaaga atataaaaag atacatatta agaaaagagt tatttttttc attttagctt
     7081 tgtgctacat attgcatttg taaaagttgt tccaaatagg tacgttcatt tgataatcga
     7141 ggaattttat gttgaccgcc taatttatct ttttctttta accaatcata aaacaattgc
     7201 ggacgggcaa cattgattat taacggattt aatgtcatgt tattgcgtct tttagcttca
     7261 taatcagaat ttaacgcttg caacgtgtcg tctactattt ttctgaaata acctatgttt
     7321 tccggaggtt ttctaaattc tattatccac tcgtgagctc cttttttctt gccttccata
     7381 aaaaccggag caacagtata atcaacaact tctgattcgg tttctaaaca tgctttggca
     7441 atagccttat ctgtgttttc aacaattaat tcttctccaa agacattgat aaaatgctta
     7501 gttcttccgg taactcgaat tcgatgtgga tttaaggaag taaatcggat ggtgtcacca
     7561 attaaatacc gccataatcc tgagtttgtt gttataacaa cggcataatt tttaaacaat
     7621 tcaacatctg ctaaacgaat tactttttga tttatagttc cgaaggtttc cattggaata
     7681 aattcataaa atattccata atccaacatc aacaataatt cattagaatt gtttaaatcc
     7741 tgaattgcaa aaaaaccttc agaagcatta tagatttcat aataattaaa atcacttctt
     7801 ggaaatagct ttttgtattg ttcgcgatag gggtcaaaac ttactcctcc atgaaaatat
     7861 acttcggcat ttggccaaat ttctaacaaa ttagttttgc cggtttcttc taaaacttta
     7921 ttcagtaaaa ccatcatcca cgacggtact cctgctaaac tagttacatc ttcataaata
     7981 gtttcttgaa cgatagccat taattttatt tcccaatcgc ccattaatga aacgcgattg
     8041 cttggagtag aactaaattc agcccaaata ggcatattgt caattaaaat tgcggataaa
     8101 tcaccaaaga aagtattatt atcttcatat aattctttgc tgccaccaag acgtaaactt
     8161 ttgcccacaa acaattgtga atcttcgttg ttatttaagt acaaactcag taaatcttta
     8221 ctagctttat aatgacaatt ttcgagagct tcattgctta cgggtataaa tttacttttg
     8281 gcatttgttg ttccgctcga ttttgcaaac catttaatgg aagtaggcca gaaaatattt
     8341 tgcattcctt ttcgggtttg ttctatcatc ggttctaaat cttcgtaact cgagattgga
     8401 atgcgttcgg aaaatacgct gtaatgtgta atggaattaa aatcatattg cctcccaaca
     8461 actgtatttt cagctgtttt taacaatgaa aataacaatt cctcttgaac ttcattcggg
     8521 tatttcataa acaattcaat ttggtgaatc cgttttttta atatccaact ggcaatggaa
     8581 ttgattatag gtaatggcat tgttaaattg ttttaatatt gtttgtgtca gagttgaaat
     8641 ttacttttac aaaacttaac tttacaacgt tatcaaaaat aataattttt agttaacgtt
     8701 gcccttttaa acttaaactt tatcaagaat gatttacgaa ggtgttctca ctaaaatgca
     8761 aacagaattg ggtaatccca tacaatatta tttagtgttt gaaaacagtt ttttaaacct
     8821 gaatcaattg ttggataaag agctggaaat cagttttcaa ggttatcaat gtttgcaatg
     8881 tggtaagaaa aagaaaattt tccgtcaagg ctattgctat gattgttata tgtcaagtgc
     8941 ttcagttgga gattggatta tgaagcctga attgagcact gctcatttgg atattgaaga
     9001 ccgtgatttg gactatgaaa ggaaagtaca attacaacca catattgttt atctagctct
     9061 ttcaagtgaa gtaaaagttg gtgttactag gggcacacaa gttcctaccc gatggattga
     9121 tcaaggagcg gtacaggcta ttccaattct ggagctacct aatcgttatc tagcaggtat
     9181 tactgaggtt gctttaaaaa acaactatgc tgataaaacg aattggcaaa aaatgctcaa
     9241 aaacgaagtt ccgcaagttg atttaataca ggaaagatta aaagcgggta actttctacc
     9301 ggatgaagtt agagattatt atgctgaaaa taatagccat ctatacgagt ttcattatcc
     9361 cgtgttaact tatccaaaaa aagtaaccag tttaagttta gataaaacac ccctttttca
     9421 aggcaaactt tccggaataa aagggcaata tttacttttt caagacggaa cggtttttaa
     9481 tgttcgaact tttgagggat atgtggtaaa gatttcatta taaaaaaaac ccgaccattc
     9541 agtcgggaaa tttttagttg tcttttttct taggttttaa taaactgttt agaccttctt
     9601 ttagattatc ttttatttct tgttctttag taaccggcgt ctttgtttta gtagtatcag
     9661 tcggttttgt attttgattt atcaaatttg taagagcaga agttccttga ttgataagtt
     9721 tttgtttttg ttgttccaca agttgattgg ctaatttcgt cgtggcttgt tttatatccg
     9781 ttgttatttt tggatttgta aaattaccac tcatcaaagc agtaattggt atattttgaa
     9841 gtttggcagc atcagccgga gataattttg ctattaaatt atttatttcg ggacctaaat
     9901 atttggttgg aacatcaaat tttacgttat aattcatcga ttgatcaaaa ccatgcgttc
     9961 caccaacgtt aacagtaatg tcttggtatt ttatatcaaa aggttttacg tttacttttc
    10021 catctttaaa agacaaagaa gtttttaaat cattcagatt caattttttt aaatcgataa
    10081 aactcacttg attgtctaag gcattcaaca acattgaatt ggaagcattt atggttgtag
    10141 aaagtaattg ccccattaaa tctcctgaaa tagattttaa atcaggtgtc atttctttag
    10201 catctaaatt tcccgaaact gtgatggtag aatttaattt tccgttaatt acattggcaa
    10261 ttggagcaat gtttttcatc atatccaact gtgtaaatgt ttcgctaatg tttacttgat
    10321 tcaaatttaa atccattttg aatgtcggaa cttttgtttt tgtggataca tttccattta
    10381 aaccaatttg tcctccaaaa atagaagtct ttatattctc tagcgtcact ttttcgtctt
    10441 ttacaatcag tttgcctgaa acatctttca agattaaatt atcatataaa accgttgttg
    10501 cttttgctgt caaagtacaa tctaaaaagg ctgggatttt cattgcttca atttcttttt
    10561 tacctttatt ttcggtttca gacgttgtca taaaatcagc taccgctagt tgatttgaaa
    10621 tcagattaaa gtttcctttt aagttttgtt tgttgaacaa ataaccataa aaattatcta
    10681 aaaacccggt gacattcata tcacttttcc ctgttttggc ctcaaatttt tgaagattca
    10741 acttacttgg attgaattga acaaccgcct cattgatatc cattcctttt ccgttttcgt
    10801 caatgtattt aaagcctgaa acactaatta aaccggcatt ttttatattt tgatattctt
    10861 ctttttcaac agattgcatg tcaaattttg tagtaacatc tgcttttaaa attcctgaaa
    10921 gcggtttgtc tacttttata ggataggctt gcgataaatt actcaaatta attgttcctt
    10981 ttaaagtcgc atcaattaaa gcattttcag ttatgttttt tatagaagct tttgcattaa
    11041 aaatgtcttg atcaatttta aacgaaagat tatcgatagc aacataggta tcattcatca
    11101 gcccggtttc gtttatgatt ttggtatcaa taatgatgtt ttcaaccgat tttggtaaat
    11161 cgggatattt gatggaggca ttagttgatg caataaccaa ctcaaatttc ggaattgttg
    11221 aatcggtata aaatcctttt gcgaaacccg aaactacaaa atcacccgat gttttaattt
    11281 tatccaaatc acctttgtag gcggatggaa ttaagcctaa aaaatttttg aacgaagaac
    11341 taggtgtgtg aaaagttaaa tcatacaact gaccgttttc aaccaattgg atgaatcctt
    11401 taaattcaag cggcaattga ttaacaaggg ctttgttgtt tttaaattca tatttacttt
    11461 tttctaaatc aatacctaaa accgcatcaa gcgtaatggc aaccttgttc atgtaattga
    11521 ttttgtcaaa gtctacactt aaatttccgg atgaagtagt gactaaatct aatgttgaag
    11581 cggcaaaatt tccttttcca ttgtgttgaa tatcattaat aattacattc attgttgaag
    11641 cttcatcaag atatttgaat tttaaattac taattgaata gttttgaata ttgagtttaa
    11701 aaggttcgct tttggatgaa gtatcgtctt tttggtcttt tagggcaatg tcgaaatttc
    11761 caataccatc tttgttaaaa atgatattta ctaagccatt attacttgaa aatgattcta
    11821 attccattgc ttcaccatca gctttgaaaa gttccttgac agacattttt aaattcaatt
    11881 caccggaata aaaaagggta tcacccgcaa agggttcttt gttgatgatg ctcaatttat
    11941 caatcgtaat gtttgctttt ggaaaatttt taaacaaact taaactgaca tcttcaaagg
    12001 caacagtagc atctactttc tcattgattg attttaaaac caattcttta attttatcct
    12061 taaataaaaa gggagtaata gctaaagcga taattacgat agctaaaaaa acacctaatc
    12121 cttttaaaat tttcttgaac atctgtttat aatttaattt ggagttactt tcttaaacga
    12181 aaagttattc ttttttgttg ttactatggt gttaatctaa tactaattct tcgcctcttt
    12241 tcaggttaaa tcgtttttga aataaatata ccgctgcata caagaagggc gtatctaaaa
    12301 gagcaaaaat tactttaaac aaaaatccac ttagcattaa cttaggaaac aagtcccaag
    12361 gtaatattct aaaactacag agcaaaaaca ataccgcaaa tgtatccacg aattgtgaag
    12421 tgaaagtgga aaaattattt cttagccaaa gcatctttcc tttggttact tttttccaaa
    12481 aatgaaaaat ataaatatca atatattgag cggttaaata ggcaatcata gaggctaaaa
    12541 cggctaaagc agtttgacca aaaacctttg aaaacattgc attatttacc ggagaacttt
    12601 caattgccgg aactgcatta gcaacatata caatcattaa cgaaaaaaca gaagcaaaaa
    12661 taccggaggt tacgacttga ttggcttttt ttctgccata tatttcagaa atgatatccg
    12721 taattagaaa ggttaaagga tatggtaaaa taccaactga aatttcaaac cgataaatgc
    12781 cgaagaaatc ccagtaaaaa aatttctgaa aaattaaatt agagacgact aatgaagcaa
    12841 taaatagagc tcctaaggca aaatagattt tataggcaaa tagtttgtct tttgtttcca
    12901 atgctttttt taacaaaaat aaacattcgt atgcaaacag ttgttaatga aaaatcaaat
    12961 agtaaagaaa aaagtccgat agaattactc aatcggactt ttttgtagac taatttattt
    13021 ttattgaatt ttcaattcaa aaggcattag catttgaatt cctttgtgtg cggttaattt
    13081 tttgacctgt tttaaagtct cataagcttc tacaccaatt tcttctttta tgattgtttc
    13141 ttttgactta gcaaatggta atttattttg atcattgata aaatccatga ttgatttatc
    13201 aaattcggga taattttgtg ttttaaattt tttaattttt gccaatgtag ccgcttcttt
    13261 taaagctaaa tctaagccac ctaattgatc aaccaaacca agttttaacg cttctgaccc
    13321 cgtccaaacg cggccttgag caacggcttc cacttgttca acagtcattt ttctnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntctact ttttgtggcg acttagcaac
    13501 atttttcgca taatccatta tgctcacact gttataatct tcgtctttat caacttttaa
    13561 tgcttttcgg atgccattat gatattgatc ttcatagcca attttatcta ttaatttttt
    13621 tgacaaagcc atttccggtg ttcgggcact tagctgattg gcaatattgt ttaatgaatc
    13681 cacacgaatg tttctgctta aagaaatatc agaaacaata gaactccaag cggaatttat
    13741 taaagccgaa atttgttcac ggtttgcctc actcatttca ttttctaaaa atggttcaac
    13801 ggcactttta tattttccgt gacgaatgac ttccattttt acgccggttt tttcctgaat
    13861 atctttaaaa aacataactt ctgtagaaag ccctttaaaa tccatttctc ctaccggatt
    13921 catataaata gtgtcagcta tagaattaat ataatattct ttttgtgtaa aaaaatcggc
    13981 ataagaaact ataaattttc ctgatttttt aaattcattt aatttgtttc gcaacgcttt
    14041 taactgagct aatcctaaat tactggtact gtttacaatt gaaattcctt taattttatc
    14101 atctatttta gcttcttcta ttgcgtttaa cacatcgatt aaaccaacag atgtctcgtc
    14161 agataagagt gtcacccatg gatctttgaa ttttccggta taatctaacg ctatattttc
    14221 catatctaat tcgatcacag aatcagattt tactttggca acatcggaat cgctgccggc
    14281 tatagcacta aatatcaata aaccaaaaaa taaaatcata cagaatacaa ataatccgat
    14341 gatagtggcc aatacatttc ctaaaaattt cattatttat tatttattta taactattaa
    14401 ttcatctaat tagtagtgaa tcattatatt atgttacaca ttatttgaaa agcttttaat
    14461 aatacagaaa cttattaaca gactcggtaa agatacttta tttctaaaat agttttagtt
    14521 attttgcttt tctatgaata gtcaaaatca agtcatttta tctttgggaa gtaatttagg
    14581 taaccgattg gaaaacattc aattggctat tgatgctatt cataattcaa tagctaccgt
    14641 ggtaaaagtg tcctctcttt atgaaactcc ggcttggggt tttgaaagta attcattcta
    14701 taattgtgct ttggtattgc aaaccttaaa accggctcca caaattttag atgaaatttt
    14761 aatcttagaa attaaattag gacgggttcg aaatgatgct gaaggctatc aagcaagagt
    14821 tatagatatt gatgtaatca cttataacga agagatcgtt gaaaccgaaa aattacaagt
    14881 gccgcaccca caaatgcaaa atcgcctttt tgttttgtta cccatgcggg atttgaattt
    14941 agattggcgt catcccatat tgcaaaaata tttaccggaa ttggttcaac tttcagagga
    15001 taaaagcaat tgtaaagttg tacaaaaatt aaaatctcct ctcgaaaaaa ttgacttaca
    15061 acaatacaat tatatcgcta ttgaaggaaa tattggtgct gggaaaacaa cattggcaac
    15121 aaagatttca gaagacttta atgcaaaatt ggttttggaa cgttttgcag ataatccgtt
    15181 tttgccaaaa ttttatgaag atcaaagtcg ctatgcattc ccgttggaaa tgtcattttt
    15241 ggccgacagg taccaacaat tatcggatga tttagctcaa tttgacttgt ttaaagattt
    15301 tattgttacc gattatcata tttttaaatc attgattttt gccaaagtta cgctggcaga
    15361 agatgaatac aggttataca aaaccatgtt tgacattatt cataaagaaa tgcccaaacc
    15421 tgatttgtat gtttatttgt atcaaaatac cgaacaactt ttgaagaata taaaaagtag
    15481 aggaagaaga tacgaacagc aaattccggc tgaatattta gataaaatca atcgaggtta
    15541 tttggattat attaaaaccc aaaccgattt aaaggtgtta attattgatg tttcaaataa
    15601 agatttcgta gaaaatcaga ttgactatgt cagtattctt gatgaaattc aacagaaaat
    15661 acagtaattt acctaataat tgaaaagtga ccttttgtgt caggtctgcc atcgcctgtt
    15721 tttaaaataa accaataatc atttgacggt aattttttcc catttaaagt accatcccaa
    15781 tcccggtttg tttggtcaat ttttttaata aatttaccat accgatcaaa aatagtcagt
    15841 gaagcatttg gaaagtattc aattccttta atggttaaaa catcattaaa accatcattg
    15901 ttcggggtga aaaatttagg aatattgatt actacaaatt gatcattgtc ttcaccacaa
    15961 tcatattttt ctctgacata agcggtatat aatcctcctt cttctacggc aaatacgttt
    16021 gaatcttgat agctgtagcc atcaattgaa aattcaaatt cccctatgcc ggaaataagt
    16081 atggtagccg tttggtttgt tactaatact tcttcaataa ttggggcagt atgttttgtg
    16141 ataaaaaaat gttttgtgtt cgaacaattc tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nntaattggg gcagtatgtt ttgtgataaa aaaatgtttt gtgttcgaac
    16321 aattctccgg aggcagggag gtaacaatga cggaatagtc cgagtcatct gtaatttgaa
    16381 tagtttgtgt ggtttctcca gtcgaccaga gataggaaac atttaaaatt ccggcatcta
    16441 acgttaacgg aataccttca caatgaattt tattttcgtc tgaaacgtca ggaatgtcaa
    16501 aaacagttac tacaaccggg agtctgtttc cggatgagca gccgttattt gttgcctcta
    16561 catagtaagt tgtattctga gtaagatttg gtgtagtgaa attagttccg gttgcaagtg
    16621 atgttccccc aaaagcggta tcaaaccagc ttattgtccc tgctgaggtt gatgcaacaa
    16681 gcaccgttga attatttcca catactgttg cgggaggagt gactgttaca attggaattg
    16741 gaataattgt ggcggtaact actgctctcg ttgctgtagt gcagttcgat tcaaaagcgt
    16801 caacataata ggatgttgtt gatgaaagaa ccggagttgt aaaggtgttt ccggttgcaa
    16861 tgggtgttcc tcctgttgaa tttgcatacc atttgacaat accatttgaa gcagttgcac
    16921 ttaagttaat tgttccatct ccacatctac tctgtgctgc tgttgaggta atcgatggaa
    16981 taacaatggt tgaactggta gaaatttgta aaataggatc tccgggcatt ccgccatatt
    17041 ctacaatata acctttgggt tggtaatttc cattgctttc tcccgtgttt gacaaatcgt
    17101 tccaagagcc gctaatgcca acgccaggag ctgtaacatg agcataattt tcatcaccta
    17161 gattattggg ttcaccacta ttccaaaaag caaaatttgg agaaaaacca tttactccac
    17221 cattccaaaa aattgtacca atttcaggac cggtcatcca acgccatacg ccttctgttt
    17281 gttcatcact accgccaatc catcctgcac cggctgcttg ctctcccgaa agttgagctt
    17341 cgtcggcagc agtaatggta gctaaataac cttgtaaccc ataatatgta cttgcttgtg
    17401 cggctgattt tgcacttgac caagtaattc caatatttgg gacatattga taatagtgac
    17461 catttgaagg taaataattg gcttgaccaa cagtgattga aaaagttctt attccggaag
    17521 gattagctga agaatttgaa aagacaacat cttttatagc tgcaattaag ttggtgtaag
    17581 taggttgcgt agaaacaccg gctaaagtta atttccctgt taaggtattc caactgctgg
    17641 taagggttgg gtgatttccg gtaagactta ataagtcttg accactcaca tatccggaag
    17701 aaatttgaat ataaatagca tcaattccgg tatcatcagg atcaacaata gtcatgtcag
    17761 taacaattct cagttgtgtt cccggacaat atacctgatt tccggtagca gttaaaatgg
    17821 gtgcaacatt ttgagaatat gaaaaaaacg gtagtgaaaa aagaactaaa taaagtagac
    17881 gaattttcat actgtaaagg tacaaattat tttgaaacct ttattttttg aaacaaatcc
    17941 caaactacaa ttcccgcact tacagagata ttaagagaat gttttgtgcc aagttgtgga
    18001 atttcaatta caccgtcaga ttcatgaata gcttcttgcg aaacaccctt aacttcgttg
    18061 ccaaaaatta aagcatattt tttttctttt tcaatttgaa aatcattgag cataattgaa
    18121 ttttctactt gttcaatggc aaaaatctgc acattttctg atttaagcct atcaattacg
    18181 gttaaaacat tttcttgata ttcccattct accgtatctg ttgcacctaa ggctgttttg
    18241 tgaatttctt tattaggagg agttgcagta attccacaca agtaaatttt ttcaatcaaa
    18301 aaagcatctg acgttctaaa aacggaacca atgttatgca gacttcttat gtcgtctaaa
    18361 attataataa tgggagtttt ttttgcgtct ttaaactcat ttatgttttt tcgatccagt
    18421 tcggaattag ctaattttct catttgacaa aattaagaaa tgaatttgaa attcaatatt
    18481 tgatattgat attgattttt gccattgaaa ctttatttcc gtatttttga atccaaacct
    18541 aaaaccaatc aacttgtcag ccaaagccac tatcgtcaaa gagcctaagg aaactccttt
    18601 aatgaaacaa tacaacgaaa ttaaacgaaa gtatccagat gcttgtttgt tatttcgagt
    18661 aggggatttc tatgaaactt ttggagaaga tgcggttaga gcttcgcaaa tattgggcat
    18721 tgtcttaacg aaacgtggag caggttctga aactgaaaca gctttggctg gttttcctca
    18781 tcattctcta aatacctatt tgcctaaatt ggttaaagct ggtcttcgag tagccatttg
    18841 tgatcaatta gaagatccaa aaatgactaa aacaattgta aaacgaggcg taactgaatt
    18901 ggttacaccg ggagtttcaa tgaacgatga agttttacag tctaaatcaa ataatttttt
    18961 ggcctcggtt tattttggaa aaaaacaaat tggaatttct tttttagatg tttctaccgg
    19021 agaattttta acggcacaag gaaatgagga atacatcgat aaattattgc aaaattttat
    19081 acccagcgaa attttagtca ccaaaaatca taaacaacat ttcagagagg ttttcgggga
    19141 agattaccat actttttttc ttgaagattg ggtttataat gaagattatg gcagggaagt
    19201 attggcaaaa cattttcaaa caaattcttt aaaaggattt ggaatcgaag atttacaaga
    19261 aggtattgtg gcatcgggag ctgttttgta ttacttgtca gaaacccaac ataataaaat
    19321 tcagcacatt acgtcaattc agcgtattgc agaagatgct tatgtttgga tggatcgttt
    19381 tacaataaga aatttagaat tataccatag tacaaatccc aatgcggtta cattgttgga
    19441 tgtcattgac aaaacgcttt cgccaatggg gtctcgtctt ttaaagcgat ggcttgcttt
    19501 gccattaaaa gatataatca aaatacaatc ccgacatgaa gtagtctctt ttttaaaaga
    19561 aaattcagat gtgctacgtc aaattcaata tcaaattaag caaatttctg atttagaacg
    19621 attgatatca aaagttgcaa ccggaaaagt atctcctcgg gaagtgatga atttgtgtta
    19681 ttcgttggac gcaattattc caattaaaac tttggcccta gccagttcaa atgaatcgtt
    19741 aagagcgata ggagatagct tgcacagttg tgatttatta cgggaaaaaa tcaaaacaac
    19801 gttgaatccc gacgctccgg tttccatcaa caaagggaat gctgttgcaa aagggattaa
    19861 tgcagaatta gatgagttaa gagcgatttc tacctccggg aaagaatatt tggaaggaat
    19921 agagagtaga gaatcacaaa gaacaggaat ttcttcctta aaaatatcgt ttaataatgt
    19981 ttttggatac tatattgaag ttcgaaatac acacaaagat aaagtcccac ccgaatggat
    20041 cagaaaacaa actttggtta atgcagaacg atatatcacc gaagaattaa aggaatacga
    20101 aacaaaaatt cttggagctg aagataaaat tcatctcata gaaacacaat tatttgaaca
    20161 attggtcatt tggattggga catacataaa acccgttcag ttaaatgccg gtttgatagc
    20221 tcaattggat tgtttgaatt ctttcacgca attggccata gataataagt atgttcgacc
    20281 gattttggat gattcatatg agttagagat taccaatggt agacatccgg tgattgaaaa
    20341 acaattaccc gttggtgttc cttacattgc caatgatgtt tttttagaca gagattctca
    20401 acaaattatt atgattaccg ggcctaatat gtcaggaaag tcggctattt tacgtcaaac
    20461 ggctttaatt gtgttattgg cccaaatggg aagttttgtt ccagcagaag ctgtacgcat
    20521 gggaattgtt gataagattt ttacccgagt aggagcaagc gataatattt cgatgggcga
    20581 atcaactttt atggtagaaa tgaatgaaac tgcttccatc ttaaataata tttctgaaag
    20641 aagtttagtg ttattggatg aaattggacg aggaacaagt acatatgacg gtatttcaat
    20701 tgcgtgggct attgctgaat atttgcatga gcatcctaat cgaccaaaaa cgttatttgc
    20761 aactcattat cacgaattaa atgaaatgga agtgacattt gaacgggtgc ataattttaa
    20821 tgtttctgtc aaggaattaa aagatacggt gctttttatt agaaaattag taaaaggagg
    20881 taccgctcac agttttggaa ttcatgtagc aaagatggca ggaatgcccc aaacagtaat
    20941 ccaaaaggct cagaaaatat taaagaaatt agaaaaaaat cattctagtg aggaattatc
    21001 cggtataaaa tctagtaaag atgaattaca gctcagcttt tttaatttag acgatccttt
    21061 attagaggaa atcaaagagg aaattttaaa cattgacatc aataccttaa ctccagttga
    21121 agcattgatg aaattgaatg aaatcaaaag aatgttgatt aaaaagtaat tgctattgaa
    21181 ttagcattca aaaagttact tttacagttt gatttttttc aaaaaaacac ttgtaaaagt
    21241 aaataattat agtacatttg catccgcatt actgaacaag tggtgctgtt cttataaaaa
    21301 tttgaatgcg aaaatagctc agttggtaga gcacgacctt gccaaggtcg gggtcgcggg
    21361 ttcgagtccc gtttttcgct caactaccta atagtacaaa taattttatt tgtatcaggc
    21421 tcgagtggtg gaattggtag acacgctgga cttaaaatcc agtgaacagt aatgttcgtg
    21481 cgggttcaag tcccgccttg agta
//
LOCUS       DAOTCW010000026        21381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1311_length_21381_cov_46.557442, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000026 DAOTCW010000000
VERSION     DAOTCW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 21381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21381
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1311_length_21381_cov_46.557442"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..493)
                     /locus_tag="P5335_03710"
     CDS             complement(149..493)
                     /locus_tag="P5335_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HRZ74018.1"
                     /translation="MKKIILIFLVSLMGINVQAQEKKNKNAKYDIAVSGNCEMCKKRI
                     EKAALSVKGVKSATWHADHQDLHLILDETKCTVQQVHEAVAKVGHDTDEIRAKDEDYE
                     KLHTCCTYERKQ"
     gene            complement(517..2751)
                     /locus_tag="P5335_03715"
     CDS             complement(517..2751)
                     /locus_tag="P5335_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ74019.1"
                     /translation="MQKQFIGYVFLLVSFISFSQEKVTGKIIYDNNQPLPGANIYWQN
                     TEIGVISDEEGKFSIPFTGEHTVLVISYVGFKTVILTILKPKTITQKMEFDSSLGEVT
                     VTNVRKSLQKSYRVTSNMQTMTGKELLKAACCNLSESFETNPSIDVNFSDAVSGSKQI
                     KMLGLTSPYILMTEENIPAIRGASQAYGLSFIPGTWVESIQITKGAGSVVNGYESISG
                     QINYELLKPADDIPFFLNAYGSTDSRFELNTHFNTKVNDKWATSLFLHGNTRISKNDM
                     NNDGFLDNPLGKQINIINRWQYSDSEKGWVSFINLRYMKDDKQTGQVDFEPDRDKGTT
                     NFWGSEISTEKLDASAKLGYVFPDTPWKSIGFQNAFNYHNQESYFGLDQYNIKQNSFY
                     SNLIYNSIISDTRHKFATGLNFTYDNYNEFVNVNDFSRIDSSLGAFFEYTFDDSDKWS
                     YVLGGRVDSHNRLGVFFTPRAHVRFKPYEKTTFRASAGRGKRSANIFAENQQLFASSR
                     QFQILDTEGKIYGLDAEIAWNYGFSFMQGFKVFGKEAEFTFDFYRTDFQNQAVVDVDN
                     SPQQVLFYNLKGQSFANSLQVDLNYQLMTHFSIRTAYKYYDVQTEYTIGLVERPLQAK
                     HRFFANLAYETHVKEKGQQWKFDFTYNWLGEQRLPQTQSNPEEYRLDAFANPFSTINA
                     QVTKTFSKTFEIYIGGENMSNYQQPNPIVGSDDPFGPYFDSSMVYGPVFGAMYYAGLR
                     FKIQ"
     gene            complement(2815..3231)
                     /locus_tag="P5335_03720"
     CDS             complement(2815..3231)
                     /locus_tag="P5335_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74020.1"
                     /translation="MTFKKYTSVLLAFYLLVSTTGLAFNVHHCGDEIASVSTVFSIEE
                     PCEGTVEKEQKTCCAKSTDNHKGCCSDEIIQADLDDSIISHFSFDFDYFSILPNVELS
                     IFWNEPIKISVQCLTYYCHANAPPLYQLHSQFLFYA"
     gene            complement(3287..4642)
                     /locus_tag="P5335_03725"
     CDS             complement(3287..4642)
                     /locus_tag="P5335_03725"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRZ74021.1"
                     /translation="MKIQFLGGAGTVTGSKTLVEHNELRILIDCGMFQGLKALRELNW
                     EPLAVDSSSIDFVLLTHGHLDHCGWLPRLVKEGFRGKIFCTSPTKQITKLILLDSAKI
                     QEEEASKANQEHYSKHEPAEPLYTVAEAENVIPFFRAVEKDIEIELTENCYFKFFQAG
                     HILGACSISLHIDGKELVFSGDVGQDDDVLMYPPEKPTTADYIFLESTYGNRIHPTND
                     VLFDLELIINQTIERGGNVIIPSFAVERAQTLMFLLWQLKKQNRIPDIPYIIDTPMGI
                     KVLDVFNDNHQWHKLAPHECEEMCRMFSMITDFEDSINAIYNKKPKVVIAASGMITGG
                     RVLSYLERYIDKLENTVMLVGYQAEGTRGRKLLEGAKEIKFYGKYYPVEAQVTLVEGL
                     SAHGDQNDLLNWLSILEKKPAKIFLVHGENEAADALRLKITERYGYNCSVPLLGQAIE
                     L"
     gene            complement(4645..5478)
                     /locus_tag="P5335_03730"
     CDS             complement(4645..5478)
                     /locus_tag="P5335_03730"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRZ74022.1"
                     /translation="MKVTKFSGEIVNFDKDKLIHSLRKSGANEAAVFQVIHEIERELY
                     EGIPTKKIYKLAFQLLKNFSNVHAARYNLRTAIQSLGPAGFFFEKFIAKVFECEGYQT
                     QTNLILEGRCVSHEVDVLLKKENCVSMVECKFHGSQDAKTDVKVPMYILSRFNDLKDR
                     DFQLFKSNYSIQNCWITTNNRFTDEAVKFASCSKLSLLSWDYPIGNSLKNKIDLNQIY
                     PVTCLTTLTMAEKDKLLVQDIITALDLIQHADWLSKIELSPNRIKNVLLEANQLCNLI
                     K"
     gene            complement(5518..5961)
                     /locus_tag="P5335_03735"
     CDS             complement(5518..5961)
                     /locus_tag="P5335_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase F system-associated protein"
                     /protein_id="HRZ74023.1"
                     /translation="MQLKQTNNWIVYSLVGFCLVALIFIRLYETQLFYDPFLGYFKND
                     YLNLPFPEYDTFLLLSNLILRYFFNTFFSLVIIFLLFKDFQLTKFAAFLYFFFFIILI
                     FLFFGILSFFDEQSNFLLFYVRRFLIQPLFLVLFIPAFCFQKKQS"
     gene            complement(5942..6493)
                     /gene="xrtF"
                     /locus_tag="P5335_03740"
     CDS             complement(5942..6493)
                     /gene="xrtF"
                     /locus_tag="P5335_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase family protein XrtF"
                     /protein_id="HRZ74024.1"
                     /translation="MKKYLVQYKPFFIFLLKFFLSYAILTFLYQIYLNKYDTTQFEVD
                     ALTKIVARQAQQTTTFFGYHSEIADNLEQSSVKFFVKEKYVARVVEGCNAISVIILFI
                     AFVIAFKGKIKQTLFFVLLGSILIYVLNIIRIALISIALYKFPAYEHLIHGVIFPLII
                     YGVVFVLWIVWVNKFSIYATKAN"
     gene            6565..7020
                     /locus_tag="P5335_03745"
     CDS             6565..7020
                     /locus_tag="P5335_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRZ74025.1"
                     /translation="MILDALKPKITEIVIQFDISTNEKLANICQLLHDEVDYYNWVGF
                     YFANHETQTLHLGPYVGAETDHTVIPFGKGICGQVAVSNQNFVVPDVSAQDNYIACSF
                     TVKSEIVVPLFVNGKNIGQIDIDSHVLNPFSETDERFLEFVNEEVAKLF"
     gene            7115..7384
                     /gene="rpsO"
                     /locus_tag="P5335_03750"
     CDS             7115..7384
                     /gene="rpsO"
                     /locus_tag="P5335_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRZ74026.1"
                     /translation="MYLTKETKAEIFTKHGGKAENTGSAEGQIALFTFRISHLTEHLK
                     KNRHDYNTERSLVLLVGKRRSLLDYLKKKDINRYREIIKELNIRK"
     gene            7525..9687
                     /locus_tag="P5335_03755"
     CDS             7525..9687
                     /locus_tag="P5335_03755"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRZ74027.1"
                     /translation="MIPNVTKEVIDLGDGRTISIETGKLAKQADGSVVVRLGDCMLLA
                     TAVSARTANPAVDFLPLTVDYREKFAAAGRFPGGFFKREARPSDSEVLTMRLVDRVLR
                     PLFPDDYHAETQVMIQLMSHDENVMPDALAGLAASAALAVSDIPFYNLISEVRVARVD
                     GKFVINPSRAQLELSDIDMMIGASLDSVAMVEGEMKEISEAEMVEAIKFAHEAIKVQI
                     HAQLRLQEAFGKKEIRTYEGEKEDEAVYAKVKAFAYDKCYAIAKEGTAKHERTAQFAA
                     VKEEAKALFTEEEYAENADLAGLVGKYFYKTNKEAVRNVILELGIRLDGRKTTEIRPI
                     WCETDYLPSVHGSSLFTRGETQALATVTLGTSREANQIDSPSEQGEEKFYLHYNFPPF
                     STGEAKPLRGTSRREVGHGNLAQRALKNMIPADCPYTIRIVSEVLESNGSSSMATVCA
                     GTMALMDAGVQMVKPVSGIAMGLITDGLKFAVLSDILGDEDHLGDMDFKVTGTKDGIT
                     ACQMDIKIDGLRYDIMEQALGQARDGRMHILGKITETIATPRADVKKHAPKIIMRTIP
                     GAFIGALIGPGGKVIQELQKATGCTIVINEVDEQGVVEILGTDPDGIAAVLAKIDSII
                     FKPTVGEAYEVKVIKLLDFGAVVEYTSAPGNEVLLHVSELAWERTENVTDVVKMGDVF
                     EVKYLGIDPKTRKEKVSRKALLPRPPRSEENKITPKKD"
     gene            9826..12243
                     /locus_tag="P5335_03760"
     CDS             9826..12243
                     /locus_tag="P5335_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ74028.1"
                     /translation="MRFSISRIFIIINLLFLTTVSGQEFSVSGILVDENKVGIESSEV
                     ILLKDGNIISSTLTDSKGTFNLKSTIGTYSLRFYFVGSVIYQTDFVLDKNISLGTLTS
                     FDNANQLKEVEVTSKKKIIENKVDRTVFNVENSVRANGSDGLELLKSTPGVAVSANSI
                     GIVGKSTVSVMIDERIINLSGDELNNYLRSINSDNIKSIEVITTPPAKYDAQGNSGLI
                     NIKLKKIQENSWSSTIRANYIQTTYPSISSAVNFIYNKNKLSLFTDFAKREGHDKIVE
                     NTDTYYENETWDGATKRKEKADIYRGILGFDYKLSENAKIGAKYVGLFDKPDMTDSNT
                     SKVFDKVTGNLISTYETTGSNDSKNMNNTFNTYYIQKLDTLGKQFTVDLDYFNYEDTK
                     IRNFTTQNFNPTNNPLGPATIAQNSGIQNIENYSGKFDFDLPTKWANYGFGGKLSWIK
                     NKSDISYYDLSTGVPFMDISQTNVYDYTENTQAAYINFSKTLSSKWQTQIGLRYENTQ
                     VVGITTSPDVTQNQENKFDYNKLFPSAYLLYTPNENNSFSINYSKRIDRPVFWDLNPF
                     KWYLSSFIIVEGNPYLQPSFSDNFEINYNYKENLTFKIYYSSTTDGNLQIPFIDLNTD
                     PQTIRYQRGNFYDKDQYGATITFLYNTFEWWESSNTLNGNYNATTFNQELPTSVQNGF
                     LYVFYTNNTFTLNKSKSFFAELNYEYHSPRKDLFYEATQYSKLDAGLRYSIKEKGWSF
                     ILLGTDIFRTYMAYISTEINNTPQRRSLYQDERMLRVGITYKFGNKKLAVNQRESGNE
                     EVQGRLK"
     gene            12321..13184
                     /locus_tag="P5335_03765"
     CDS             12321..13184
                     /locus_tag="P5335_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRZ74029.1"
                     /translation="MRQLKITKQVTNRETASLDKYLQEIGKVDLITADEEVELAQRIK
                     AGDQRALEKLTKANLRFVVSVAKQYQNQGLTLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKIGSINKINKMYALLEQSNERPPSAEEIAK
                     ELDMTVNDVKESMKNSGRHLSMDAPLVEGEDSNLYDVLRSGESPNPDRELIHESLRTE
                     IERSLETLTPREADVVRLYFGLGDQHPMTLEEIGETFDLTRERVRQIKEKAIRRLKHT
                     SRSKILKTYLG"
     gene            13422..14081
                     /gene="rpe"
                     /locus_tag="P5335_03770"
     CDS             13422..14081
                     /gene="rpe"
                     /locus_tag="P5335_03770"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRZ74030.1"
                     /translation="MKNTLIAPSILAADFANLQRDIEMINQSEADWFHIDIMDGVFVP
                     NISFGMPVLEAITKHAKKTIDVHLMIIDPDRYIKTFAQLGSTNFTVHYEACTHLHRTL
                     QAIKAEGMKAGVALNPHTPVSLLEDIIKDIDLVCLMSVNPGFGGQSFIEHTYTKVKQL
                     KEIILLNNASTLIEIDGGVTNKNAQQLVEAGADVLVAGNYVFKAQNPIETIKDLKKIT
                     Q"
     gene            complement(14103..14522)
                     /locus_tag="P5335_03775"
     CDS             complement(14103..14522)
                     /locus_tag="P5335_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRZ74031.1"
                     /translation="MKKQVPVSTIMSKNVIKLNLSDDLTKAEMLFKKHHIRHIPVVDG
                     NKIIGMLSYTDLLRISFVDAVDDEDEVVDVTVYNMFTVEQVMAKKLVTVSPETTIKQV
                     AEILATKEFHALPVVEGKLLVGIVTTTDIIKYLIDQY"
     gene            complement(14629..15408)
                     /locus_tag="P5335_03780"
     CDS             complement(14629..15408)
                     /locus_tag="P5335_03780"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HRZ74032.1"
                     /translation="MNPFILLSVFFGVFVGKAIEPVYVYFYGKPLFIHFYPIPKKLKP
                     FELEILNSNFHYYSNLSPRKKKYFDHRVATFIQNYQFIPKEGIEISTEMKILIAATAI
                     KLSFGMRNYLVKVFDKIIIYPESYYSTVNDVWHKGEFNPRMKAIVFSWEDFLAGYEYD
                     NDNLNLGLHEFAHAYHLHGLKSSDVSALIFAETYKQIISYTAKPEIRTKLIASNYFRI
                     YAYTNSFEFIAVLLEHFFETPEQFEIEFPELFQKVRKMINF"
     gene            complement(15510..15583)
                     /locus_tag="P5335_03785"
     tRNA            complement(15510..15583)
                     /locus_tag="P5335_03785"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15547..15549),aa:Arg,seq:tcg)
     gene            complement(15625..16074)
                     /locus_tag="P5335_03790"
     CDS             complement(15625..16074)
                     /locus_tag="P5335_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRZ74033.1"
                     /translation="MSLQSQINEEIKNAMRAKDTIALESLRAVKSAILLALTEGGAKD
                     ELAQEDEIKLLQRLVKQRKDSAAIYVQQGRQDLAEPELLQSAVIEKFLPKQLSENEVE
                     VAVAQIIADNGFSGMASMGQLMGIASKQLGGSADGKTISTVVKKLLA"
     gene            complement(16221..18143)
                     /gene="ftsZ"
                     /locus_tag="P5335_03795"
     CDS             complement(16221..18143)
                     /gene="ftsZ"
                     /locus_tag="P5335_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRZ74034.1"
                     /translation="MISNSDFGSISFDLPKNQSNVIKVIGVGGGGSNAINHMFKQGIK
                     GVDFVVCNTDSQALQNSPVPNKIQLGVNLTEGLGAGANPEVGQQSALESMEDIDKLLD
                     SNTKMIFITAGMGGGTGTGAAPVIAQMAKEKDILTVGIVTIPFQFEGKVRSEQAMSGV
                     ERLRKQVDSLIVINNNKLREVYGNLGFKAGFSKADEVLATASRGIAEVITHHYTQNID
                     LKDAKTVLSNSGTAIMGSATAEGENRAKDAIVSALDSPLLNDNKITGAKNVLLLIVSG
                     SSEITIDEIGEINDHIQAEAGHNANIIMGVGEDESLGDSIAVTIIATGFNVEQQKEIV
                     NAEPKKIIHSLEENQTNTIDLSQKVVSPILDFVIEKETPSSNIEDKIVFELIDEMEVV
                     EPKMVSDLIPTSELIKNIEITYEIVAPFTENQLFATTPEIREIIVKDPEFVIVEKEQV
                     TLSFDLGINQPIESEKTILFDLNPTATNEIIVNEPMQVIPMTEVNETGVIRYSLEEYM
                     EVEAELLNSKPVVNVVEEPVNEEMNITVKKVETPIAFSPSFDEINPTEMTIEETLKMR
                     ADDRRKKLKEFNYKFHTNSSRLDEMEKVPAYKRMGVDLPTNAPLNNQSRMSLGTDSND
                     DTQLRSNNSFLHDNVD"
     gene            complement(18197..19558)
                     /gene="ftsA"
                     /locus_tag="P5335_03800"
     CDS             complement(18197..19558)
                     /gene="ftsA"
                     /locus_tag="P5335_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRZ74035.1"
                     /translation="MKNENIAVGLDIGTTKIVAMIGRKNEYGKLEILGVGKSKSLGVH
                     RGVVNNITQTIQSIQTAVAEAEENSGYMIKDVVVGIAGQHIRSIQHSDYISRSNPEEV
                     ICGKDIEQLINQVHKLAMLPGEEIIHVLPQEFKIDGQSEIKEPIGMYGGRLESSFHVV
                     VGHAASIRNVGRCIQSAGIELSGLTLEPLASSDAVLSQEEKEAGVALIDIGGGTTDLA
                     IFKDGIIRHTAVIPFGGNVITDDIKEGCSIIEKQAELLKVKFGSAWPGENKDNEIVSI
                     PGLRGREPKEISLKNLSKIINARVVEIIEQVFDEIKSYGHDDPKKKLIAGIVLTGGGA
                     QLKHIKQLVEYITGMDTRIGYPNEHLAGNSDEEMSSPLYATAVGLMMNSINNNTKSAV
                     LMSEMEQSKDKEAKIEEPKVEPIAEAPKATEQKNESTENKIRRSFFDKYVEKIKEFLD
                     NAE"
     gene            complement(19565..20290)
                     /locus_tag="P5335_03805"
     CDS             complement(19565..20290)
                     /locus_tag="P5335_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HRZ74036.1"
                     /translation="MKKYFSWTNIRLLLILLVVCFLYSFMSKRNENRKLQKATVEFLD
                     GEQLFVTHETVNKLLIENKKDASSIQKVALDLNNLENTINRHEMVENAEVFVSVDGNL
                     KAVVKQKTPVARVFESHDSYYIDYKGTKMPLSSIHTARVPLVSGEINVNNSKKLDELF
                     RIIHNDEFLKKNIIGIQIMPSGSLIMKNRNFEYDIDFGKTINMERKFKNYKAFLQKAV
                     LDSSIYSYKNINLKFTQQVVCTK"
     gene            complement(20274..>21381)
                     /locus_tag="P5335_03810"
     CDS             complement(20274..>21381)
                     /locus_tag="P5335_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRZ74037.1"
                     /translation="THSEWNYFIERDYSIKKRAEVLGIITKDTYCFAVAGTHGKTTTS
                     SILGHILYESGVDLTAFLGGIVENYNSNLIGNGKTVTVVEADEFDRSFLQLYPDMACI
                     TSMDADHLDIYGDKASIEATFVEFANKVEDKNLLFIPKNLPLEGIKIAINEEADFTAF
                     NIRVENSAYVFDVKTPSETIKNIQFNLPGRHNLMNALMALAMAITYGSPTNSIVRALG
                     TFKGIKRRFSYQIKQEKLVFIDDYAHHPTEINAVHQAVRELYPQQKVLAVFQPHLFSR
                     TKDFADDFAKSLSHFDEVILLDIYPARELPMEGITSAWLLSKIENSNKKLVLKNELID
                     EVLKSTATVIVTIGAGDIGELVKLLKERLNEKVF"
BASE COUNT         7142 a         3789 c         3343 g         7107 t
ORIGIN      
        1 aaaaaagatg taatttcttt ttgttttctt actactctgt tagaaaaggc cattttttca
       61 actaaaaatc gggtcaaaat tagttcattt atatcttaaa aaaataaaaa aaataaaaaa
      121 atccgatatc ggtatcggat ttttataatt attgttttct ttcatacgta cagcaagtat
      181 ggagtttctc gtaatcttca tctttagctc taatttcatc cgtatcatga cctacttttg
      241 cgactgcttc gtgaacttgt tgaactgtac atttagtttc atctagaatc aaatgcaaat
      301 cttgatgatc agcatgccaa gtagctgatt ttactccttt cactgataga gctgcctttt
      361 ctattctttt tttacacatc tcacaattgc cactaacagc aatgtcatat ttggcatttt
      421 tgtttttttt ctcttgtgcc tgaacattga ttcccattaa ggaaaccaag aatattaaaa
      481 ttattttttt cataatagaa ttgaattttg aatttgttat tgaattttaa atcgtaaacc
      541 ggcatagtac atagctccaa aaaccgggcc ataaaccatt gaactgtcaa aataaggacc
      601 aaaaggatcg tcacttccaa caattggatt cggttgttga taattactca tattctcacc
      661 gcctatgtat atttcaaaag tttttgaaaa agtttttgtt acttgtgcat ttatcgttga
      721 aaacggattg gcaaaagcat ctaaacggta ttcttccgga tttgattgtg tttgcggtaa
      781 tctttgttcg cctaaccaat tgtaagtaaa atcaaacttc cattgttgcc ctttttcttt
      841 cacatgtgtt tcgtaggcta aattagcaaa aaaacgatgt ttagcctgca aaggtctttc
      901 tactaaacca attgtatatt ctgtttgaac atcataatat ttatatgcag ttcgaattga
      961 aaaatgagtc atcaattgat agtttaaatc aacttgtaaa ctgttagcga atgattgtcc
     1021 tttaaggttg taaaacaaaa cctgttgtgg actgttatct acatcaacca ctgcttgatt
     1081 ttgaaaatct gttcggtaaa aatcaaaagt aaattctgct tctttaccaa aaactttaaa
     1141 accttgcata aaactaaacc cataattcca agcaatttca gcatctaaac cgtaaatttt
     1201 tccctcagta tccaaaattt gaaattgtct ggatgaggca aataattgtt gattttcagc
     1261 aaaaatattg gcacttcttt tccctcttcc tgccgatgca cgaaaagtcg ttttttcata
     1321 tggtttgaaa cgaacatgtg ctcttggagt aaaaaaaact cctaaacggt tgtgagaatc
     1381 tactcgacca cctaaaacat aactccattt gtcagaatcg tcaaatgtat attcaaagaa
     1441 agcccctaat gaactatcaa ttctgctaaa atcgttcaca tttacaaact cattataatt
     1501 atcataagta aaattaagtc cggtagcaaa tttatgacgc gtatcactta ttattgagtt
     1561 ataaatcaaa ttcgaataaa aactattttg tttgatatta tattgatcca atccaaaata
     1621 agattcctgg ttatggtaat taaacgcgtt ttgaaaaccg atacttttcc atggtgtatc
     1681 cggaaaaaca taccctaatt tggctgaggc atctaacttt tcagtactaa tttcagaacc
     1741 ccaaaaatta gttgttcctt tatctctatc cggctcaaaa tcaacttgac cggtttgttt
     1801 atcgtctttc atatagcgaa gattgataaa acttacccaa cctttttctg aatcggaata
     1861 ttgccaacga ttgatgatat tgatttgttt tccgagcggg ttatccaaaa aaccatcatt
     1921 attcatgtca tttttggaaa tccgagtatt tccatgcaga aataaacttg tagcccattt
     1981 atcattgact ttagtattaa aatgagtgtt caactcaaat ctagaatctg tcgatccata
     2041 agcattcaaa aagaatggaa tatcatcggc aggttttaat aattcataat taatttgacc
     2101 tgaaatactt tcgtaaccgt ttacaactga accggctcct ttggtaattt gaatactttc
     2161 cacccaagtt ccggggataa aagaaagtcc gtaagcttgt gaagcacctc gaatagccgg
     2221 aatgttttct tcagtcatta aaatataagg actggttaat cccaacattt taatttgttt
     2281 actaccggat actgcatcag aaaagttaac atcgattgac ggatttgttt caaaactttc
     2341 agacaaatta caacatgccg cctttaacaa ttccttgcct gtcatggttt gcatgtttga
     2401 agtaacccga taactttttt gaagtgattt tctaacattc gttacagtca cttcacctaa
     2461 agaactatcg aactccattt tttgagttat cgttttaggt ttcaaaatag ttaaaataac
     2521 tgttttaaag ccaacataac taatgactaa aacagtgtgt tctcctgtga aaggaataga
     2581 gaactttcct tcttcatctg atatgacgcc tatttcggta ttctgccagt aaatattggc
     2641 tccaggaagt ggttgattat tatcgtatat tattttgccg gttacttttt cttgagaaaa
     2701 tgaaataaaa gaaaccaata aaaaaacata ccctataaat tgtttttgca taaaaaatga
     2761 ttaaaaatta aacaagaagt tttcactaac tagtgaaatg atgtttaatt taaatcaggc
     2821 gtaaaaaagg aattgtgaat gtaattggta gagcggaggt gcatttgcat gacaataata
     2881 tgtcaaacat tgtactgaaa ttttaatcgg ctcattccaa aaaatagata attcaacatt
     2941 aggtagtatc gaaaaataat caaaatcaaa actgaaatgt gaaattatac tgtcgtctaa
     3001 atcagcttga atgatttcat cagaacaaca acctttatgg ttatccgttg acttagcaca
     3061 acatgttttt tgctcttttt caactgttcc ttcacagggt tcttctatac taaaaaccgt
     3121 ggacacagaa gcaatttcat caccacaatg gtgcacgtta aaagccaatc ccgtggtaga
     3181 aaccaagaga taaaaagcga gcaaaacact tgtgtacttt ttgaaagtca tgggacaaaa
     3241 gtacattttt ttagttcaat aattttacga aataatgtta aatatactac aattcaatag
     3301 cttgtcctaa aagagggaca ctacaattgt atccatatct ttccgttatt tttaatcgca
     3361 aagcatctgc cgcctcattt tctccgtgta ctaaaaatat ttttgctggc tttttttcta
     3421 atatactaag ccaattaagc aaatcattct ggtcaccatg tgcagaaagt ccttccacta
     3481 aagttacctg agcttctacg ggataatatt tcccgtagaa tttaatttct ttagctcctt
     3541 ctaatagttt tctacctcga gttccttctg cttgatagcc tacgagcatg accgtgtttt
     3601 ctagtttatc aatatatctt tctaaataag ataaaactct tcctccggtt atcattccac
     3661 tggccgcaat tacaaccttt ggttttttat tataaattgc gttgattgaa tcttcaaaat
     3721 cggttatcat tgagaacatt ctacacattt cttcgcattc atgcggtgcc aacttatgcc
     3781 attgatgatt atcattaaaa acatccaata cttttattcc catcggtgta tcaataatat
     3841 aaggaatgtc gggaattctg ttttgctttt tcaattgcca cagtagaaac attaaggttt
     3901 gtgcccgttc aacagcaaaa ctgggaataa tgacattccc tcctctttca atcgtttggt
     3961 tgatgatcaa ttctaaatca aaaagcacat cattagttgg atgaattcga ttgccatagg
     4021 tactttctaa aaaaatataa tccgcagtag ttggtttttc cggaggatac atcaatacat
     4081 catcgtcttg cccaacatca ccggagaaca ctaattcttt gccatcaata tgtaatgaaa
     4141 ttgaacaagc tcctaaaata tgaccagcct ggaaaaattt aaaataacaa ttttcagtga
     4201 gttcaatttc aatatctttt tctactgctc taaaaaaagg aatcacattt tcagcttcag
     4261 caactgtgta tagtggctct gccggttcgt gtttagaata atgttcttgg tttgctttgg
     4321 atgcttcttc ttcttgaatt ttagcactat ctaataaaat aagttttgtt atttgcttag
     4381 taggacttgt acaaaatatt ttaccccgaa aaccttcttt tactaacctg ggtaaccatc
     4441 cacagtgatc caaatggcca tgtgtgagca atacaaaatc aatactggaa gaatctactg
     4501 ctaagggttc ccaattgagt tctcgcaaag cttttaaccc ttgaaacata ccacaatcaa
     4561 tcaggattcg aagttcgtta tgctctacaa gtgtttttga accggttact gttccagcac
     4621 ctcctaaaaa ttgaattttc atggttattt tattaaatta cacaattgat ttgcttcaag
     4681 caatacgttt ttgattcggt ttggacttaa ctcaattttg gaaagccaat cagcatgttg
     4741 aattaaatcg agtgcggtaa ttatatcttg aactaataat ttatcttttt cagccattgt
     4801 tagtgttgtt aaacaagtca ccggataaat ttggtttaaa tctattttat tttttaagct
     4861 gtttcctatc ggataatccc aacttaacaa actcagcttt gaacaactag caaattttac
     4921 agcttcatct gtaaatcgat tattggtagt tatccagcaa ttttggatgg aataattgct
     4981 tttaaacagt tgaaaatctc tatcttttaa atcattaaat cgggataaaa tatacatcgg
     5041 aactttcaca tccgttttag catcttgact tccatgaaat ttacattcaa ccatggaaac
     5101 acaattttct tttttaagca atacatcaac ttcatgtgac acacaccttc cttccaagat
     5161 gagattagtt tgtgtttgat atccttcaca ttcaaaaacc ttagctataa atttttcgaa
     5221 gaaaaaacct gccggtccta acgactgaat agccgttctt agattatacc gagcagcatg
     5281 aacgttagaa aaatttttta acagctgaaa ggctaatttg tagatttttt tggttggaat
     5341 accttcgtat aattctcttt caatttcatg aattacctga aaaactgctg cttcattcgc
     5401 tcctgatttt cgcaacgaat gaattaattt atctttatca aagttgacaa tttcacctga
     5461 aaatttagtg actttcatac ccgactcttt agtcttataa agatacgaaa aattgagcta
     5521 agattgtttc ttctgaaaac aaaatgcagg aataaaaagc actaaaaaca aaggttgaat
     5581 tagaaatctt ctaacataaa aaagtaaaaa attactttgc tcatcgaaaa aagataggat
     5641 accaaaaaag agaaaaatta aaataatgaa gaaaaaaaag tataaaaatg cggcaaattt
     5701 tgtaagctga aaatccttaa acaaaagaaa aatgattacc aatgaaaaga acgtattgaa
     5761 aaaatacctt aaaatcaaat ttgacaataa taagaaagtg tcatattcag gaaaaggtaa
     5821 attaaggtaa tcgtttttga aataacccaa aaaaggatca taaaaaagtt gggtttcgta
     5881 taatctaatg aatatcaagg caactaaaca gaaacctact aacgaataga caatccaatt
     5941 attagtttgc tttagttgca taaattgaaa atttattaac ccaaactatc cataaaacga
     6001 aaacaacacc ataaataata agcgggaaaa tgacaccatg aatcaaatgt tcatatgccg
     6061 gaaatttata caaggctatt gaaattaatg caattcgaat aatattcaat acataaatca
     6121 gaattgaacc gagtaacaca aaaaacaacg tttgctttat ttttccttta aaagcaatta
     6181 caaaagcaat aaataaaata atgacactga ttgcattaca tccttcaact acccgagcca
     6241 catatttttc tttgacaaaa aacttaacag aactttgctc aagattatcc gcaatttctg
     6301 aatgataacc aaaaaatgta gttgtttgtt gtgcttgacg ggcaacaatt ttagttaatg
     6361 catccacttc aaactgagtt gtgtcatatt tattgaggta aatttgatac aaaaaagtaa
     6421 gaatggcata gcttaaaaag aatttaagca gaaaaataaa aaaaggttta tattgtacaa
     6481 ggtatttttt caaaacttta tttttaacaa aaatatagaa ttttgagcag ttatgatgtt
     6541 ttacctttgt aaaaaaacaa taccatgatc ttagatgccc taaaacctaa aattactgaa
     6601 attgtcattc aatttgatat ttctaccaat gaaaaactgg caaatatttg tcaattactt
     6661 catgatgaag ttgattatta caattgggtt ggattttatt ttgccaacca tgaaacacaa
     6721 actctgcatt taggacctta tgttggtgct gaaacagacc ataccgttat tccttttgga
     6781 aaaggcatct gtggacaggt agccgtttct aatcaaaatt ttgttgtgcc ggatgtttcc
     6841 gctcaagaca actatattgc ctgcagcttt acagttaagt ctgaaattgt tgtcccactt
     6901 tttgttaatg gtaaaaacat aggacaaatt gacatcgaca gtcatgtttt aaatccgttc
     6961 agtgaaactg acgaacgctt tttagaattt gtaaatgaag aagttgcaaa gttgttttag
     7021 aaaaaacttg tacttttaaa aaataaaatt acctttgcaa ccgaattagg aaaaccctga
     7081 ttcatacata ataatcattt aatataatat tggaatgtat ttaactaaag aaacaaaagc
     7141 tgaaatcttc actaaacacg gaggaaaagc tgaaaacaca ggttctgctg agggacaaat
     7201 tgcattgttc actttcagaa tttcacactt aacagagcac ttgaaaaaaa atcgtcatga
     7261 ttacaatacc gagcgttcgt tagtattatt ggtaggtaaa agaagaagtc ttttagatta
     7321 ccttaagaaa aaagatatca acagatatcg tgagattatt aaagaattga atattagaaa
     7381 ataatcaata ggaaagaggt gcgaaagtgc ctctttttgt tttatagatt ttgacagaaa
     7441 aaagtttggt ttttcattgg gttacacaac aactacacac aacaacacaa cgaaacctaa
     7501 tattaatacc aaattagaat ttttatgatt ccaaatgtaa caaaagaggt tatcgattta
     7561 ggagatggca gaaccatttc tatcgaaacc ggaaaattag cgaaacaagc agatggttct
     7621 gtggtggttc gtttaggcga ttgtatgctt ttagcaacag ccgtttctgc cagaacggca
     7681 aatccagcag tagatttttt acccttaaca gtagattatc gtgaaaaatt tgccgcagca
     7741 ggacgtttcc cgggtggttt tttcaaaaga gaagcacgtc caagtgatag tgaagtatta
     7801 actatgcgtt tagttgaccg tgttttacgt ccactctttc ctgatgatta tcatgctgaa
     7861 acacaagtga tgattcaatt aatgagtcat gatgaaaatg tgatgccgga tgcattagca
     7921 ggtttagccg cttctgcagc cttagctgtt tcagacattc cattttacaa cttaatttca
     7981 gaagtacgtg tagcacgtgt agatggaaaa ttcgttataa atccaagtag agcacaatta
     8041 gagttatccg acattgacat gatgattgga gcttctttgg attcagttgc catggttgaa
     8101 ggtgaaatga aagaaatttc agaagccgaa atggttgaag ccattaaatt tgctcacgaa
     8161 gccattaaag ttcaaattca cgctcaattg cgtttacagg aagcatttgg taaaaaagaa
     8221 atccgtacat acgaaggtga gaaagaagac gaagccgttt atgctaaagt aaaagccttc
     8281 gcttatgata aatgttatgc tattgctaaa gaaggaacag caaaacatga aagaacagct
     8341 caatttgcag ccgtaaaaga agaagcaaaa gcattattta ctgaagaaga atatgctgaa
     8401 aatgctgact tagcaggttt agttgggaaa tatttctaca aaaccaataa agaagctgtt
     8461 cgtaacgtaa tccttgaatt aggaattcgt ttagacggaa gaaaaacaac tgaaatcaga
     8521 ccaatttggt gtgaaacaga ttatttacct tctgttcacg gttcttcttt atttactcgc
     8581 ggagaaactc aagcgttagc aaccgtaact ttaggaacct caagagaagc caatcaaatt
     8641 gactcaccat ctgaacaagg tgaagagaaa ttctatttac actataactt ccctcctttc
     8701 tcaacaggtg aagcaaaacc gttaagagga acatccagaa gagaagttgg tcacggaaac
     8761 ttagctcaac gtgcattaaa aaatatgatc ccggctgatt gtccttatac tatccgtatt
     8821 gtttcagaag tgttagaatc aaatggttct tcttccatgg caaccgtttg tgcaggaaca
     8881 atggctttaa tggatgcagg tgttcaaatg gtaaaaccgg tttccggaat tgccatgggc
     8941 ttaatcactg acggcctaaa atttgcagtt ctttcagata tcttaggtga cgaagatcac
     9001 ttaggagata tggactttaa agttaccgga acaaaagacg gaatcactgc ttgtcaaatg
     9061 gatatcaaaa ttgacggttt acgttatgat attatggaac aagcgttagg ccaagcacgt
     9121 gatggtcgta tgcatatttt aggtaaaatt accgaaacca ttgctacacc aagagctgat
     9181 gttaagaaac atgctccaaa aatcattatg cgaactattc cgggagcatt tattggtgct
     9241 ttgattggac ctggaggaaa agttattcaa gaattacaaa aagccaccgg ctgtacaatc
     9301 gttatcaacg aagtagatga acaaggtgtg gtagaaattc ttggaactga tccggatgga
     9361 attgcagctg tattagctaa aattgattcg attatcttca aaccaactgt tggcgaagct
     9421 tacgaagtga aagtaattaa gttgttagat tttggtgcag tggtagaata tacttcagct
     9481 ccaggaaatg aagtcttact tcacgtatct gaattagctt gggaacgtac agaaaatgta
     9541 actgatgttg ttaaaatggg tgatgttttt gaagtgaaat atttaggaat tgatccaaaa
     9601 acaagaaaag agaaagtgtc aagaaaagca cttttgccta gacctccaag atcagaagaa
     9661 aataaaatta ctccaaaaaa ggattaattt taaccaactc atatctcaac ccgattcaaa
     9721 aactgaatcg ggttttttat gatttaattt gtaatttatc tactacaaag cattgtggtc
     9781 ttaacattta ttttatattt gcagtaaagc gattcaaaaa taaatatgag attttcaatt
     9841 tcgagaattt ttatcataat taatctgtta tttttaacaa ctgtttccgg tcaggagttt
     9901 tctgtttccg gcatattagt cgatgaaaac aaagtaggta ttgaatcttc agaagtcatc
     9961 cttttaaaag atggaaacat catcagttca actttaactg attccaaagg aacttttaac
    10021 ctaaaaagca ctataggaac ttattccctt cgtttttatt ttgtaggctc agtcatttat
    10081 caaacggact ttgttttaga caaaaatatt tctttgggaa cattgacatc ctttgataat
    10141 gccaatcaat taaaagaagt agaagtcact tctaagaaaa aaattattga aaacaaagtc
    10201 gaccgaactg tttttaatgt tgaaaatagc gttagagcta acggaagtga tgggctagaa
    10261 ttattgaaaa gcacaccggg ggtagccgtt tctgcaaatt ctattggtat tgttgggaaa
    10321 agcacagtaa gtgtgatgat tgacgaaaga attatcaatc tgagcggaga tgaattgaac
    10381 aattatttac gaagcatcaa ttctgataac attaaaagta tcgaagtaat tactacacct
    10441 ccggcaaaat atgatgccca agggaatagc ggattaatca atatcaaact aaagaaaatt
    10501 caagaaaatt cctggtctag tacaatacga gccaattata ttcaaactac ttatccatct
    10561 atttcatctg ccgttaactt tatttataac aaaaataaat tgagcttatt taccgatttt
    10621 gcaaaaagag aaggccatga taaaattgta gagaacacag acacttacta tgaaaatgaa
    10681 acatgggatg gagcaacaaa acgaaaagaa aaagcggata tttacagagg aatacttggt
    10741 tttgattata aattatctga aaatgccaaa attggggcaa aatatgttgg cctatttgat
    10801 aaaccggata tgaccgacag taatacctct aaagtttttg ataaagtcac cggtaatctg
    10861 atttctactt acgaaacaac aggtagtaat gattccaaaa atatgaataa cacctttaac
    10921 acctattata ttcaaaagct agacacgtta ggaaaacaat tcactgttga tttagattat
    10981 tttaattatg aagatactaa aatcagaaac tttactacac aaaacttcaa tccaacgaac
    11041 aatcctttag gcccggcaac tatagctcaa aattccggta ttcaaaatat tgaaaattat
    11101 agtggaaaat ttgattttga tttaccaaca aaatgggcaa attatggttt tggcggaaaa
    11161 ttaagttgga taaaaaacaa aagtgatata agctattatg acttatcaac cggagttccg
    11221 ttcatggaca ttagccagac taatgtttat gattacacag aaaatactca agcagcctat
    11281 attaattttt ccaaaacatt aagttcaaaa tggcaaacac aaattggttt gcgttatgaa
    11341 aatacacaag tagtcggcat tacaacatca cctgatgtta ctcaaaatca agaaaataaa
    11401 tttgattata acaaactttt cccttctgct taccttttat atacacctaa tgaaaacaat
    11461 agtttttcaa ttaattacag caaaagaatt gaccgccctg ttttttggga tttaaatccg
    11521 tttaaatggt atttaagttc ttttattatt gttgaaggca acccttatct acaaccctct
    11581 ttttcagata attttgaaat taattacaat tataaagaaa atttaacttt caaaatttat
    11641 tattcatcca caacggatgg aaatcttcaa attccattta tagatttaaa tacggatcca
    11701 caaaccattc gttatcaaag aggcaacttt tatgacaaag accaatatgg agctactatc
    11761 acctttttat ataatacttt tgaatggtgg gaaagttcta atactctgaa tggaaattac
    11821 aatgctacca ctttcaatca agaactgcca acttcagttc aaaacggatt tttgtatgtt
    11881 ttttacacaa acaatacctt tactttaaat aaaagtaaat cgttttttgc cgagttaaat
    11941 tacgaatacc acagtccaag aaaagactta ttttatgagg ccacgcaata ttcaaaactt
    12001 gatgccggat tacgttattc cattaaagaa aaaggttgga gtttcatttt attaggaacc
    12061 gatattttca gaacatatat ggcctatatt tctacagaaa taaataacac tcctcaaaga
    12121 cgttcacttt accaagatga acgtatgtta cgagtgggaa ttacttataa attcggaaat
    12181 aaaaaactcg ctgtaaacca acgagaatct ggcaacgaag aagttcaagg tagattaaaa
    12241 taatttttta ttattatttt gtaacttttt ataaatgctt tgcgtataat cattcgaacc
    12301 tattattcaa atagaattaa atgagacaac tcaaaatcac aaagcaggta acgaatcgtg
    12361 aaactgcatc ccttgacaaa tacttacaag aaattggaaa agttgattta attacagccg
    12421 atgaagaggt tgaattagca caacgtatca aagcaggtga ccaacgagcc ttagaaaaat
    12481 taactaaagc aaacttacgt ttcgttgtat cggttgctaa acaataccaa aaccaaggat
    12541 taaccttgcc ggatttaata aacgaaggaa atttagggct tattaaagcg gctcaacgtt
    12601 ttgatgaaac gcgtggattc aaattcattt cctatgccgt ttggtggatt cgtcaatcga
    12661 ttttacaagc tttggcagaa caatcccgta ttgtacgttt accgttgaac aagattggtt
    12721 ctatcaataa aatcaacaaa atgtatgctt tattggagca atcaaatgag cgtcccccat
    12781 ctgcagagga aattgcaaaa gagttagaca tgactgtaaa tgatgtaaaa gagagcatga
    12841 aaaactccgg gcgtcattta tccatggatg ctcctttagt tgaaggtgaa gattctaatt
    12901 tatatgatgt tttacgctca ggagaatcac caaatccgga tagagaattg attcatgaat
    12961 cacttcgtac agaaattgaa cgttcgttag aaacccttac accaagagaa gcagacgtag
    13021 ttcgtttata ttttggatta ggagatcaac atccgatgac tttagaagaa attggcgaaa
    13081 catttgattt aacacgtgaa cgtgttcgtc aaattaaaga aaaagctatt cgcagattaa
    13141 aacatacttc aagaagtaaa atattaaaaa cataccttgg ttaatctgtt taacctataa
    13201 aaagtaacaa cagttaataa ctgttcgttt tttgattgat gattgaaact ccggctgatt
    13261 accggagttt tgctttttta gggcaagcaa aactcacggt cagttcgcta cgctcgggtt
    13321 tccggagttt tgcttttttg tgggaaacaa aacttttagt cctttaaact tttaaacttt
    13381 taaactaaaa tctatctttg caaccaacaa caccactcaa aatgaaaaac acgcttattg
    13441 ccccttctat attagccgct gattttgcca atttacaacg cgatattgaa atgataaatc
    13501 aaagtgaggc tgattggttt cacattgaca tcatggatgg tgtttttgtt cccaatattt
    13561 cttttggaat gcctgtttta gaagccatca ccaaacacgc aaaaaaaact attgatgttc
    13621 atctaatgat tattgacccc gataggtaca tcaaaacttt tgctcaatta ggctcaacaa
    13681 actttacggt acattatgaa gcttgtactc atttgcatag aacactacaa gctatcaaag
    13741 cagaaggaat gaaagctggt gttgctttaa atccacacac tccggttagt ttattagaag
    13801 atattattaa agatatagat ttggtttgtt tgatgagtgt aaatcctgga tttggcggac
    13861 aatcatttat agagcacacc tacactaaag taaagcaact aaaagaaatt attttactca
    13921 ataatgcttc aactctaatt gaaattgatg gtggtgttac caataaaaat gcacaacaat
    13981 tggtagaagc cggagcagat gttttagtag ccggcaatta tgtatttaaa gcccaaaatc
    14041 caattgaaac aatcaaagat ttaaagaaga ttacacagta attttttcgg cgtttatttt
    14101 tcttaatatt ggtcgattaa atatttgata atatccgtag tggtaactat cccaactaat
    14161 aatttcccct caactacagg aagtgcatga aattctttag ttgccaatat ttcagccact
    14221 tgtttaattg ttgtttcagg acttacggtg actaattttt tagccatcac ttgttctacg
    14281 gtaaacatat tataaaccgt tacatcaaca acttcatctt cgtcatcaac agcatctaca
    14341 aatgaaatac gaagcaaatc agtatagctt agcattccaa taattttgtt gccatctacg
    14401 accggaatat gacggatatg atgttttttg aatagcattt cagcttttgt caaatcgtct
    14461 gaaagattta acttaatcac atttttggac attattgtcg atacaggaac ttgctttttc
    14521 atttcaataa cttttaatat attaaaacaa atttcacctc tttttagcta taataagatg
    14581 acgactatca gtaaataatt gaatttattt tagtatatta tggggtagct aaaaatttat
    14641 catttttctt actttttgaa acaattccgg aaattcaatt tcaaattgtt ccggagtttc
    14701 aaaaaaatgt tccagcaaaa ctgctataaa ctcaaaagaa ttagtgtagg cataaattct
    14761 aaaataattg gaggcaatta gttttgtcct aatttcaggt ttggcagtat aagaaatgat
    14821 ttgtttatag gtttcagcaa aaattaaggc actaacatcc gaagatttca acccgtgaag
    14881 atgataagca tgagcaaatt catgcaagcc taaatttaaa ttatcattat catattcata
    14941 acctgctaga aaatcttccc atgaaaacac aatggctttc attcttgggt taaattcacc
    15001 tttatgccat acatcgttta ctgtagaata atatgattcg gggtaaataa taattttatc
    15061 gaaaacctta accagataat ttcgcatccc aaaagataat ttaatggcgg tagccgctat
    15121 taaaattttc atttcagttg atatttcaat gccttctttt ggaataaatt gatagttctg
    15181 tataaaagta gctacacgat ggtcaaaata ttttttcttt cggggagata aatttgaata
    15241 atagtgaaaa tttgaattca atatttcaag ctcaaaaggt tttaattttt ttggaatagg
    15301 ataaaaatga ataaacaacg gttttccata aaaataaaca tagacaggtt caattgcttt
    15361 tccaacaaaa acaccaaaaa aaacagacag aagaataaaa ggattcaata cttatatttt
    15421 atttttaaat atagataaaa accactactt attagtaatt tcaagatgaa aataaaaaaa
    15481 cacccaaaaa ttatttgggt gtttcaaggt gacctcggag gggttcgaac ccccaaccat
    15541 cagagccgaa atctgatatt ctatccagtt gaactacgag gccaatgatt gcattttcga
    15601 tgtgcgaatc tacaataaaa tatattatgc taaaagtttt tttactactg ttgaaatcgt
    15661 ttttccatca gcactgcctc ctaattgttt tgacgcaatt cccattaact gccccataga
    15721 agccattccg gaaaatccat tatcagcaat aatttgagca actgcaactt ctacttcatt
    15781 ttcgcttaat tgttttggca aaaacttttc gattacagca gactgaagta attccggttc
    15841 tgctaaatct tgtcttcctt gttgaacata aattgccgca ctgtctttac gttgttttac
    15901 taaacgctgt aatagtttta tttcatcttc ctgtgctaac tcatcttttg ctccaccttc
    15961 agttaaagct aacaaaatag ccgacttaac tgctcttaac gactctaaag cgattgtgtc
    16021 ttttgctcgc atcgcatttt taatttcctc gtttatttgg ctttgtaaac tcatagtagt
    16081 tgttttttta tataactatt tataaaaaga aatccttaat ccattaccta agattcttgt
    16141 tttaatggat tgcaaagata aaaaaataac ccgaaaatta gattctaatt tccgggttaa
    16201 atcatgcttg tttgagttag ttaatccaca ttgtcatgaa gaaatgaatt gttagaacgc
    16261 aactgtgtat cgtcgttgct atctgttcct aaagacatcc tggattgatt atttagaggt
    16321 gcatttgttg gtaaatctac acccattctt ttgtatgccg gtactttttc catttcatct
    16381 aaacgagatg aattggtatg gaatttataa ttaaattctt ttagtttttt tcttctatca
    16441 tccgcacgca tttttaatgt ttcttcgatg gtcatttccg ttggatttat ttcatcaaag
    16501 gaaggagaaa aagcaatagg agtctccact ttttttacgg taatgttcat ttcttcattt
    16561 accggttcct caacaacatt aaccactggt tttgaattca gaagttctgc ttcaacttcc
    16621 atgtattctt ctaaggaata ccgaattaca ccggtttcat ttacttcagt cattgggatg
    16681 acttgcatag gttcatttac aataatttcg tttgtggcag taggattgag gtcaaaaagg
    16741 atggtttttt cagattctat aggttgattg attcctaaat caaacgaaag tgtcacttgt
    16801 tctttttcaa caattacaaa ctcaggatct ttcactataa tttcgcgaat ttccggtgtt
    16861 gttgcaaaca attgattttc agtaaaagga gctacaattt cataggttat ttcaatgttt
    16921 ttgattaact cagaagtagg aatcaagtct gataccattt ttggttctac gacttccatt
    16981 tcatcaatta attcaaaaac tattttatct tcaatattac tagaaggtgt ttctttttca
    17041 atcacaaaat ctaaaattgg cgaaaccact ttttgtgata aatcaatcgt gttggtttgg
    17101 ttttcttcta aagaatgtat aattttttta ggctcagcat tcacgatttc tttttgctgt
    17161 tccacattaa aacccgttgc aatgattgtt acagcgatag aatctcctaa agattcatct
    17221 tcaccaaccc ccattataat atttgcattg tgtccggctt cagcttgaat atgatcatta
    17281 atctccccta tctcgtcaat tgtaatttca gaagagccgg aaacgataag caacaatacg
    17341 tttttagccc ctgtaatttt attatcattc aacaaaggag aatctaatgc agaaacaata
    17401 gcgtctttag ctctgttttc tccttcagca gtagcagaac ccattattgc agttccactg
    17461 ttagacagaa ccgttttagc atctttcaag tcaatatttt gtgtatagtg atgcgtaatc
    17521 acctcagcta taccacgaga agcagtcgct aaaacttcat ccgctttaga gaatccggct
    17581 ttaaagccaa gatttccata cacttctctc aatttattat tattgataac gataagtgaa
    17641 tcaacttgtt ttcttaaacg ctccactcct gacatggctt gttctgaacg aactttacct
    17701 tcaaattgaa atggaattgt tacaatacca accgttaaaa tatctttttc tttagccatt
    17761 tgagcaatca ccggagcagc tcctgtacct gttccaccac ccattcctgc cgtgatgaaa
    17821 atcattttgg tattgctatc caataattta tcaatgtctt ccatgctttc taaggcagat
    17881 tgttgtccta cctcaggatt agctccagcc cctaatcctt ctgtcaggtt tacacccaat
    17941 tgaattttat ttggcactgg actattttga agagcttgtg agtcggtatt acaaactaca
    18001 aaatctactc ctttaatccc ttgtttaaac atgtggttaa tggcattact tccgccacca
    18061 cctacaccaa ttactttaat tacatttgat tggtttttag gtaaatcaaa tgaaatactt
    18121 ccaaaatctg agttgcttat catactactt ggtttttcta attcatttat ttttaaaact
    18181 ttacagaact taggttctat tctgcattgt ctaaaaattc tttaatcttt tctacatatt
    18241 tatcaaagaa agaacgtctg attttattct cggtactttc atttttttgc tccgttgctt
    18301 ttggggcttc ggcaatgggc tcgactttag gttcttcaat tttagcttct ttgtctttag
    18361 attgctccat ttcagacatt aaaacagcac tttttgtatt attattaatg ctattcatca
    18421 ttaatccaac agcagtagca tataatggac tagacatttc ttcatcggaa tttccggcta
    18481 aatgttcatt aggataacca attcgggtat ccattccggt aatgtattct actaattgtt
    18541 tgatatgctt caattgtgca ccacctccgg tcaatacgat tccggcaatc aatttctttt
    18601 tgggatcatc gtgtccgtat gatttaattt catcaaaaac ctgttcaata atttctacca
    18661 cgcgggcatt aattatttta gaaagatttt taagcgaaat ctctttgggt tcacgtcctc
    18721 ttaatccggg aatagaaaca atttcattgt ctttattttc accaggccaa gctgagccaa
    18781 atttcacttt taataattct gcttgttttt caataattga acaaccttct ttgatatcat
    18841 ccgtaattac atttccaccg aatggaatta cagctgtgtg ccgaatgatg ccatctttaa
    18901 aaatagctaa atcggttgtt ccaccgccta tatcaattaa agccacaccg gcttcttttt
    18961 cttcttgact caaaacagca tcagatgagg ctaatggttc caatgttaaa ccggataact
    19021 caattccggc actttggata catcttccca catttctgat tgaagcagca tgaccaacaa
    19081 ctacgtgaaa actgctttct aatcgtccac catacattcc gataggttct ttgatttcag
    19141 actgaccgtc gattttaaat tcttgtggta aaacatgaat aatttcttcc ccgggcaaca
    19201 tggccaattt gtgcacttga ttgattaact gctcaatatc ctttccgcaa attacctctt
    19261 ccggattact acgactgata taatcactat gttgaatact tcgaatgtgt tgaccagcaa
    19321 ttccaaccac aacatcttta atcatgtagc ctgaattttc ttctgcttcc gctacagcgg
    19381 tttgaataga ttggatagtt tgcgtaatat tattgacaac tcctctgtga acacccaaac
    19441 ttttggattt tcccacacct aagatttcga gtttaccata ctcgttttta cggccaatca
    19501 tggccacaat ttttgtggta ccaatgtcta aacctactgc gatgttttcg tttttcatgt
    19561 gtttctattt tgtgcaaact acctgttgcg tgaacttcag attaatattt ttgtaactgt
    19621 aaatcgaact atctaaaact gctttttgta aaaatgcttt ataatttttg aattttcgct
    19681 ccatgttgat ggtttttcca aaatcaatgt catattcaaa atttctattc ttcataatta
    19741 agcttccact cggcataatt tgtatcccaa tgatattttt tttcaaaaac tcatcgttgt
    19801 ggattattcg aaataattcg tctaattttt tactgttatt tacatttatt tcccctgaaa
    19861 ccagcggtac acgtgccgta tgaatacttg acaatggcat cttagtgccc ttgtagtcaa
    19921 tgtagtagga atcatgactt tcaaacactc tggctaccgg tgttttttgt ttaacaacag
    19981 cttttaaatt cccatcaaca ctcacaaaaa cctctgcatt ttcaaccatt tcgtgtcggt
    20041 tgatggtatt ctccaaatta ttcaaatcta aagcaacttt ctgtatactg gaagcatctt
    20101 ttttattttc tattaacaat ttattaaccg tttcgtgcgt aacaaaaagt tgttccccat
    20161 ctaaaaattc taccgttgct ttttgcaatt ttcgattctc atttcttttt gacatgaaag
    20221 aatacaagaa acaaactacc agtaaaatca acagcaaacg aatatttgtc caactaaaat
    20281 actttttcat tcaatcgttc ttttaatagt ttaaccaatt ctcctatatc tcctgccccg
    20341 atagtgacaa tcacggtagc tgtacttttc aaaacttcat caatcaattc attttttaag
    20401 actaattttt tattggaatt ctcaatcttt gacaacaacc aagcagaagt aatcccttcc
    20461 attggtaatt ctctcgcagg ataaatatcc aacaaaataa cttcatcaaa atgcgagaga
    20521 ctttttgcaa aatcatctgc aaaatctttc gttctactga acaaatgcgg ctggaaaaca
    20581 gctaacactt tctgttgggg atacaattct cttacggctt ggtgaaccgc atttatttca
    20641 gtaggatgat gagcataatc atcaataaaa accaattttt cttgtttaat ctgataggaa
    20701 aatctacgtt taattccttt aaatgttccc aaggctctga cgatactatt ggttggggaa
    20761 ccatatgtta tagccatcgc caaagccatg agtgcattca ttaaattgtg tcttcccggt
    20821 aaattgaatt gtatattttt aatcgtttct gatggtgttt taacatcaaa aacataagca
    20881 ctattttcga ctctaatatt aaatgctgta aaatctgctt cttcattaat ggcaattttg
    20941 attccttcca atggtaaatt tttaggaata aacagcaagt ttttatcctc tactttatta
    21001 gcaaattcca caaaagttgc ttcgatagaa gctttatcac cataaatatc taaatgatcc
    21061 gcatccattg aggtaataca agccatatcc ggatacaact gtaaaaacga acgatcaaat
    21121 tcatcagctt caacaactgt tactgttttt ccgttcccaa ttaggtttga attataattt
    21181 tcaacaattc cacctaaaaa tgcggttaag tcaaccccag attcataaag aatatgtcct
    21241 aaaatactgg aagtagttgt ttttccatgt gtaccggcta cggcaaaaca ataagtatct
    21301 ttagtaatta tccctaaaac ttcagctctt ttttttatcg aataatcgcg ttcaataaaa
    21361 taattccatt ccgaatgagt a
//
LOCUS       DAOTCW010000027        21250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1320_length_21250_cov_47.571503, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000027 DAOTCW010000000
VERSION     DAOTCW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 21250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21250
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1320_length_21250_cov_47.571503"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2814
                     /locus_tag="P5335_03815"
     CDS             <1..2814
                     /locus_tag="P5335_03815"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRZ74038.1"
                     /translation="NPIAKITNYTRSQFNADPQFWTMCEDNQGITIFGNNDGAVFFDG
                     NHWNKINLPNNSSVRSLLKTKSGKIYAGGYNEFGTLQKNEFGQYYYTSLIEELQLTNK
                     HIENVWQIQEINNSIICRTFNGLIVITGKTALFIPCNQSFLNSAVINNRYFVHDANHG
                     IYSYNIVDKELELVFEAGKFNNEEISAMHHFGKPNTILLFTKSGSIYSGNTLNKEVVK
                     IVSLFNENQNDQITCAVQKNAQTILIGTLNSKVIEFSSNGNLNSHNPSYSGLKNIAIH
                     NLYKSKDNFWVLYNNGLSFVDYNPSYINLFDKASVYDVLIHNSKIYLATNSGVYYADM
                     SLNNIPGTYYNFIKLNGIQGQTWSVTLASNDVIIGHDNGLFVLDGLKPIKIGSESGFW
                     KITPIEGNENSYLASNYTGLFLLKKIADKWEIIHKIKGFDESARDILPSGEKNTYWIC
                     HGYQGVFKVHLNQDYTKAIAVEHFTDKNGLKSFYNVNVTKWKNEIIFTTNTGIYSYNA
                     VKNKFLLHSKLNNILDPTKNTRKLITYGSKTWFVSDDEAGYFYTNTKDKSIHKDLFLN
                     LKGYFNRGMECIVPISENQIVFGSNLGLFFYNLKNNLSTTTVPTLLTSIHYNVNQKQL
                     ASALDNSKTTEFPNKTDGLRFEYAAPEMTKGTQIQFSHKLDPIDKSWSAWQNTSSKEY
                     THLSPGTYTFMVKSRNLSGINGKETTYRFTILPKWYQTNFAFILYILFSVLFIYFISF
                     RIRKKIEKERKKAHLEAEKTKKLLELELEQLKLIRDKEKINAVKIHLEEDVIEKSKQL
                     ANYTLLLSQKKELFNDLQNDLKNLREINKNDETRKKITQIFQKLHQNKIGEEYMEIFD
                     VNFEKVNHNFFEKLKEINPTLTKRELRLCAFVKMDLSNKEIAPLLNISIRGVENARYR
                     ARKKLNVQHEENFVSYLISLINE"
     gene            complement(2865..3623)
                     /locus_tag="P5335_03820"
     CDS             complement(2865..3623)
                     /locus_tag="P5335_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRZ74039.1"
                     /translation="MSAAKNINITLKIWRQKDASDKGGIETYKLDNVSTASSFLEMLD
                     QLNEQLVNDKKEPVAFDHDCREGICGACSLYINGRAHGPDTGITTCQLHMRMFNDGDT
                     IFVEPWRSKAFPVIKDLVVDRTAFERIQQAGGFVSVNTSGNTIDANSIPIPKHDADNA
                     FMAAACIGCGACVATCKNGSAMLFVGAKVSQYALLPQGRVEATERVLNMVRQMDEEGF
                     GNCTNTGACEIECPKGISLENIARLNREYLKASF"
     gene            complement(3630..5639)
                     /locus_tag="P5335_03825"
     CDS             complement(3630..5639)
                     /locus_tag="P5335_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRZ74040.1"
                     /translation="MKLDSKIPEGPVAQKWTNHKNHLKLVAPNNRPKIDIIVVGTGLA
                     GASAAASFGEMGYNVSAFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIK
                     GGDYRAREANVHRLAEVSGSIIDQCVAQGVPFAREYGGYLDNRSFGGTQVQRTFYAAG
                     QTGQQLLLGAYSALSRQIGLGRVKMHNRHEMLELVKVDGKARGIIARNLITGELERHS
                     AHAVIIATGGYGNVYFLSTNAMGCNVTASWKIHKQGALFANPCFVQIHPTCIPVHGSN
                     QSKLTLMSESLRNSGRIWVPKKKEDAEAIRAGKLKPTQLEEQDRDYFLERRYPAFGNL
                     VPRDVASRAAKERCDAGHGIEANDTNEGVYLDFSTEIKSKGKQAAYGQGNHNPTEEEI
                     IKLGKKWLEEKYGNLFTMYQKITDENPYETPMKIYPAVHYTMGGVWVDYNLQSTIPGC
                     FVAGEANFSDHGANRLGASALMQGLADGYFVLPYTVSNYLADEIRTGKISTDLPEFAE
                     AENNVKSAIDKFLSNNGSKTVDHFHKRLGLIMWNKVGMGRNEQGLKDAITEIAQLKEE
                     FYKDVFIPGSANDLNPELEKALRVADFMELGQLMAMDALQRNESCGGHFREEYQDAEG
                     ETLRDDVNFKFVGAWEYKGADISKAELHKEELNYEEIKIAARNYK"
     gene            complement(5658..6524)
                     /locus_tag="P5335_03830"
     CDS             complement(5658..6524)
                     /locus_tag="P5335_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRZ74041.1"
                     /translation="MAKSALLKSSIAKKYWMALTGLFLCLFLVGHLAGNLQLLMPDSR
                     LQFNQYALFMTSNPIVKILSYVTYISILFHAIDGIILTVQNKKARPIGYAKSDAGANS
                     SLPSRSMAVLGTLILVFIATHMVNFWARMHFDEKMPLMTHVIEVSPGVKNVFYVGKND
                     GQYYQVDQVLKEGESVENNPQKQLIIKNKTELYNAQVNVKIADLYKDLHLITIDFFKD
                     KKYGLYFTLFYVFSMLVLGFHLWHGFQSAFQSLGLNSPKWTPTIKFVSKAFAVIVPLL
                     FAIIPLYIHFSK"
     gene            complement(6681..7562)
                     /locus_tag="P5335_03835"
     CDS             complement(6681..7562)
                     /locus_tag="P5335_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74042.1"
                     /translation="MKLQWKITGLLLLIVASIGIFSCSTENETSKQPNLIIKFKFDQN
                     QVRLNNLGQPSLIPIGNGSQSPDMQKISAHYIELSPSVNTLLGQGKVLYHAPETDLGG
                     AMAIDFSQAKIVGQNEVFLKIPITPQLAGAYNWIRVSLSYQSGNIQVYSQGSTYTGTL
                     ESFVGYNTYIGTHSIGNNVFPVNGNREQGYWAFALNSLPFATSGQAPAGATTVPNPLF
                     QTSPIPQGSCVVTGKFETDLTITGNETKDIEVTLSLSTNASFEWTEVTTDDKFEPAIG
                     ENVVDMGLRGLIPSFVK"
     gene            complement(7564..8421)
                     /locus_tag="P5335_03840"
     CDS             complement(7564..8421)
                     /locus_tag="P5335_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HRZ74043.1"
                     /translation="MKKINLSLCFFLVFQTIGWAQNKIAYQLFTKEGKKTTYKKLFKE
                     AVTADVVLFGEYHDNSVVHWLQLELTKDLAEKMKLVLGAEMLEADNQKQLNQYLKGEI
                     TQKQFDSTARLWKNYKTDYKPLVDFAKEKKFPFVATNIPRRYASMVFKNGLEELEKLS
                     AEEKNWIAPLPILFDINLPGYQNMMSMQGGHAGDKMPKAQAIKDATMAHFIVQNLQLG
                     TLFIHYNGTYHSDNFEGINWYLKKYKPELKIITIATVEQKDIAKLAKEEYNKADFILV
                     IDEDVTKTY"
     gene            complement(8408..11206)
                     /locus_tag="P5335_03845"
     CDS             complement(8408..11206)
                     /locus_tag="P5335_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRZ74044.1"
                     /translation="MIKNGFLSFVFFLLVNQSWSQNNSLVQNIAFNSVGPTIMSGRVV
                     DVDVNPDNPIEFYVGYASGGVWYSNNNGMSFTPIMDNSATQNVGEIAVDWKSSTIWVG
                     TGEHNSSRSSYAGIGILKSTDKGKTWQNMGLKDSHHIGKIEINPTNPNEVVVGVTGHL
                     YSKNMERGVFKTNDGGKTWKQTLFIDDETGIIDIAVSSKNFNIQFASAWKKDRKAWNF
                     IGNGTTSGIYKSEDGGSTWKLISTTESGFPTGEGVGRIGLATFDDAIVYAVLDNQSKR
                     PKKIQTKTTMPEMFSAPGEVFMNIPNSEINAYLKKQGLSEKYRAENIKNWIQNGNMEP
                     AEAKKIMQDANAALFETEVIGAEVYKSIDGGKTWKKTHSDYIDDFYYSYGYYFGRIAV
                     DLSNSDKLYVSGVPIIKSDDGGKTFVSINRENVHADHHIVWVNPKKAGHLINGNDGGL
                     NISYDDGANWIKCNPHGVSQFYHVNVDYQKPYNIYGGMQDNGVWVGQNNYEYSVEWHQ
                     EGRYPYESILGGDGMQTQIDNRNPSIVFTGFQFGNYYRIDRSNESRKYIAPKAKKEEK
                     PLRFNWQTPILLSSHNQDILYLGSHYLHRSFNQGDTWSVISPDLTQGGKDGNVPYGTI
                     STLSESPFQFGLLYTGSDDGLVHVSKDAGASWQKISNSFPKDLWVSRVVASRHQKERV
                     FVTLNGYRSDDFTPYIYVSEDYGQTWKSIATNIPTAAVNVIIEDDKNSSILYVGTDNG
                     LFISFNRGDNWQPFMNGLPPVAVHDLVIQTEAKDLIVGTHGRSIYKVNLKSVQQLNEE
                     TINSALKIFACDEIYYSLNWGSSWSKWFNPNEPKLVVDYYTKNQGEVLVQVINEIGIV
                     VFEEKQMATSGINQWTYMVEISKSGAEKWHKKDKKLILKEAKNGKTYLVSGKYQIKMT
                     QKSYSQSTPLNIIRNEKN"
     gene            11332..12624
                     /locus_tag="P5335_03850"
     CDS             11332..12624
                     /locus_tag="P5335_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRZ74045.1"
                     /translation="MKYHSIDRNLYIKNRTKFTSQMKPNSVAIFNSNDNYPVSADTTL
                     PFAQHRDILYLSGVDQEESILLLFPDAPYENLKEVLFLRETNEHIAVWEGEKLTKERA
                     FEVSGIKNVIWVQDFHKTLKEVMSYADTMYINTNEHYRASVETETREARFIKWWKENY
                     PAHKVEKSNPILQRLRSIKESEEIDLIQKACDITEKGFRRVLSFIKPNVMEYEIEAEF
                     AHEFLRNRSKGFAYTPIIASGNNANVLHYIENNQQCKAGDLILLDVGAEYANYSSDMT
                     RMIPVSGRFTARQKEVYNAVLRVKNEATKMLVPGTLWKQYHVEVGKIMTSELLNLGLL
                     DKADVQNENPDWPAYKKYFMHGTSHHLGLDTHDYGLLHEPMQANMVFTVEPGIYIPKE
                     GFGIRIEDDMVIQNEGEPFNLMRNIPIEVEEIESIMNA"
     gene            12702..13484
                     /locus_tag="P5335_03855"
     CDS             12702..13484
                     /locus_tag="P5335_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRZ74046.1"
                     /translation="MKSIHYKNTKINYTDTGKGTAIVLLHGFLENLSMWKNYIADFSK
                     KNRVIIIDLLGHGNSECLGYIHTMEDQADMVHEVLHELKIRKAVFIGHSMGGYVALAF
                     AELYPDVMKGLVLLNSTSRSDSAERKLNRERAILAVKQNYTAFVRMSIANLFNEDNRE
                     RLAVEIELVKMEALKTPLQGIVAALEGMKIRKDREVLLHFSPYPMMLILGKKDGVLNY
                     EESLDQIKDSKVELVTFPDGHMSYIENETELKMVLLNFLKKI"
     gene            complement(13528..16287)
                     /locus_tag="P5335_03860"
     CDS             complement(13528..16287)
                     /locus_tag="P5335_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRZ74047.1"
                     /translation="MKANSFLSLLANHILTNHSFEMESLTIVLPNKRARVFLLEELKQ
                     TIQSNSFAPRIINIEDFIQEIATIRTIDSIELLFEFYSVYLSVTNKNEQQSFEQFSNW
                     GKTLLQDFNEIDRFLLSPNHVFSYLKEIEAIKRWNLELQDKTRLIENYLQFWDNMPLY
                     YDSFYKHLLNKGIGYQGLIYREAVENLNHFSNTIGNHHFIFAGFNALNKAEEMIFQHL
                     LSIEKATVLWDAEETFLNDPYHDAGLFARKIKKSWPFYKNNPYQWIFNEFKEPKNIQI
                     MGTPKTIGQAKLAADIIQTIADQQPLEKVAIVLGEENILIPLLNSLPNSVSHLNITMG
                     YSGKNNPAQLLLSKLFKMHQNAFKRSQSSYVFYYKDVLEVLQNPLVEPFVNAKTLVNQ
                     INQYNYTFISHVKLVEMYNQSNSFFQLLFEKWPANTVQILDTLATVLLQIKQFLQSED
                     SKDKITNSFVYAIFKIINKLSTYCSEHNYITSIEGLHGLYKQIIDLAEVSFEGEPLQG
                     LQIMGVLESRVLDFETVIITSMNEGVFPAGKSQNSFIPLDVKRELGLPTFKEKDAIYT
                     YHFYHLLQRAKNIYLLYNTESEGIDAGEKSRFITQLEVEKQPNHVLSHQIYNAILPEK
                     AHEPMVVEKSELVMERLREMADNGFSPSTLTTYIRNPMQFYFQRILRISETEEVEENI
                     AINTLGTIIHAALEVLYKPYMNTFLSISNVENMLKIADSEVFKQFKTVYKEGEITKGK
                     NLLAFEVAKRNVYNFLQLEKRLLEQDDAVKVLFLEKNFEAVLQDVRLPFPIKIKGNVD
                     RIELRNGKLRIIDYKTGKVEQNGLAIQNWNGLTADIKHDKIIQLLCYAFMVQAEFPNT
                     EMEVGIISFKNLKAGFMPFQIKEGREVILNQVDDSVLEDFKTELIELLNEILNVEIPF
                     EEKIL"
     gene            complement(16352..17734)
                     /locus_tag="P5335_03865"
     CDS             complement(16352..17734)
                     /locus_tag="P5335_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRZ74048.1"
                     /translation="MKHLNKLVLAIFMFAGLSSQAQDSDNPWAFTFGVNALDGGRVSA
                     AGSVADQFSEYFNAKDYWSVVPSVSYLSVSRYVGDNFSFGLTGSFNRLEKFVQPRVDG
                     EYVVADVDLMYYGVDAVVNYSIGKFLKAFEPTVHVGGGYTFLGDNSAGTVNGGLGLNI
                     WLSETIGLGLRSTYKHSFEDDRSVMPTHMQHFAGVTFKFGGKDTDGDGIYDKDDACPE
                     VFGLKQYNGCPDTDLDGIIDGSDACPDVFGLAEFQGCPDTDGDGIADKDDSCPEVKGP
                     KELNGCPDTDGDGIADKDDKCPDVKGPKENQGCPWPDRDGDKVFDKDDRCPDVAGTVA
                     NNGCPEISEAILSSLRDYAKTILFESGKATFQQRTFTVLQNITGILKEYPNARFSIEG
                     HTDSDGKDASNQKLSEDRAAAVKNYLIENGINASRLESKGFGESMPIDTNKTKMGKAN
                     NRRVEVKLIK"
     gene            complement(17848..19041)
                     /gene="kbl"
                     /locus_tag="P5335_03870"
     CDS             complement(17848..19041)
                     /gene="kbl"
                     /locus_tag="P5335_03870"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRZ74049.1"
                     /translation="MYGKIQQHLQNELKTIEDNGIFKKERIITSPQGAEITISTGETV
                     LNFCANNYLGLSSHPEVIQAAKDALDSHGFGMSSVRFICGTQDIHKQLEKTIAHFYGT
                     EDTILYAAAFDANGGVFEPLFNEEDAIISDSLNHASIIDGVRLCKAARYRYENSNMAD
                     LEQQLIKANEEGRRFKLIVTDGVFSMDGLVAPLDKICDLADKYDAMVMVDECHAAGFI
                     GATGKGTMEAKGVMGRVDIITGTLGKALGGAMGGYTTAKKEIIEILRQRSRPYLFSNS
                     LAPSIVGASLKVFELLEKDTALRDKLEWNTNYFKKGMKDAGFDIIDGDSAIVPVMLYD
                     AKLSQVMANELLKKGIYVIGFFFPVVPKDKARIRVQLSAAHSKEHLDKAIKAFTEVGK
                     ILNII"
     gene            complement(19056..>21250)
                     /locus_tag="P5335_03875"
     CDS             complement(19056..>21250)
                     /locus_tag="P5335_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRZ74050.1"
                     /translation="LYNAFLKNITPLSLLNRVSQELHKIQDEQNILSIAEFNAIIHNE
                     IQNQPAPFIYERMGERYKHFFIDEFQDTSQMQWENLIPLIDNALSSEDLGGERGSLMI
                     VGDPKQSIYRWRGGKAEQFIELSKTKNPFVNPDKKLFHLDTNWRSYSEVIDFNNQFFQ
                     FLSNEFQYPDYKDLYENHSHQKENSKKGGYVNLSFIPSKSDWEEDVEKDELYLEAILQ
                     TIQKVQSKNFAFRDMVILTRKKEPGVKIATFLTENSIPIVSSETLLLQNSSEVSFIIA
                     LLRFLKNGEDKEAKAHFLYYIGTKRQSNSSVHDFIAMGMSFITESALENWLASIQLIF
                     SFQKLRKKSLYEVVESCIKTFIQPKQTNAYIQDFLDRVLDRDIKNQSGISDFLLYWEE
                     NAHRFSIPSPEGNNAIRIMTIHKAKGLEFPVVLFPYAEENYSKSPKDKLWIEPESDLI
                     SLPKILVDNNKSVEEIGDSAAQVFHQKKEEELLDNINILYVAMTRAEEQLYIISGMNL
                     TSKGEISQNNMSSFFLKFLEEKGLLDRHKTTYEWGSESRISSSGKKTQELKPIPLVQE
                     VLNSESIKIAQRESLMWGTHQQKAIEYGNVIHEILSYIKTRNDIEWAISKALEGGLIS
                     VSQKEEVKRIIIQVCSHPELNLFFDETNKTLNEQTILRKSDSIIKPDRIVFNQQNQAM
                     LLDYKTGEPNLKHQKQLEIYETALHNMGFKVIKKALVYIGETLEIVTL"
BASE COUNT         6872 a         3842 c         3298 g         7238 t
ORIGIN      
        1 aatccaattg ctaaaatcac aaattatact cgttcccaat ttaatgcaga tccacaattt
       61 tggaccatgt gtgaagacaa tcaaggaatc accatttttg gaaataatga tggtgcggtc
      121 ttctttgatg gaaatcattg gaataaaatt aatctcccaa ataattcatc tgtaagaagt
      181 ttactgaaaa caaaatccgg taagatttat gccggtggtt ataatgaatt cgggacatta
      241 caaaaaaatg aattcggtca atattattac acttccctga ttgaagaatt acagttaaca
      301 aacaaacaca ttgaaaatgt ttggcaaatt caagaaataa acaactctat catttgtaga
      361 acttttaatg gattgattgt tattacaggc aagactgcac tatttattcc ttgtaatcaa
      421 tcttttctta attccgccgt gattaacaat cgctattttg tacatgatgc caaccacgga
      481 atatatagtt ataacatagt agacaaagaa ttagaacttg tttttgaagc cggtaaattc
      541 aacaacgaag agatttcagc catgcaccat ttcgggaaac caaatactat tttgcttttc
      601 actaaatcgg gctccattta ctctggcaat acacttaata aagaagttgt aaaaatcgtt
      661 tcgttattta atgaaaatca aaacgaccaa attacttgtg ctgttcaaaa gaatgctcaa
      721 acaattttga ttggtacact taattctaaa gtaatagaat tcagcagcaa tggtaattta
      781 aactcccaca atccttccta cagcggatta aaaaacattg ccattcataa tctatataaa
      841 tccaaagaca atttttgggt tttatacaac aacggtttat cgtttgtaga ttataatcca
      901 tcttatatta atctttttga caaagcatcc gtttatgatg ttttaataca taactcaaag
      961 atatatttgg caactaattc aggtgtttat tatgcggata tgtctttgaa taatattccg
     1021 ggaacttatt ataatttcat caaattaaat ggaattcaag gccaaacctg gagcgtaaca
     1081 ttagcatcaa atgatgtaat aatagggcat gataatggtt tattcgtatt agacggtcta
     1141 aagccaatta aaattggcag tgaatcaggg ttttggaaaa taacccccat tgaaggaaat
     1201 gaaaactcct atttagcctc aaattataca ggcttatttt tgttaaaaaa aatagctgat
     1261 aaatgggaaa taatacataa aattaaaggc tttgatgaat cagcaagaga cattcttcca
     1321 tcaggtgaaa agaataccta ttggatatgt catggctacc aaggcgtgtt taaagtgcat
     1381 ttaaatcaag attacacaaa agcaatagct gtagagcatt ttactgataa aaatggttta
     1441 aaatcatttt ataatgtaaa tgtgacaaaa tggaagaacg aaattatttt tacaaccaat
     1501 acaggtattt atagttacaa tgctgtaaaa aacaaatttt tattacactc taagcttaac
     1561 aatattcttg atccaacaaa aaacactcga aaattaataa catatggttc aaaaacatgg
     1621 tttgtttctg atgatgaagc aggttatttc tatacaaata ctaaagataa atccatccat
     1681 aaagatttgt ttttgaactt aaaagggtat ttcaatagag gaatggaatg tattgtacct
     1741 atttcagaaa atcagattgt ttttggttcc aatctcgggt tattttttta taatttaaaa
     1801 aacaatttat ccactacaac agttcctacg ctgcttacct ctatccatta taatgtaaat
     1861 caaaaacaac ttgcatcagc acttgataat tctaaaacca cagaattccc aaataaaaca
     1921 gatggtttgc gttttgaata tgcagcacct gaaatgacaa aaggaacaca aatccaattt
     1981 agtcataaac ttgacccaat agataaatct tggtcagctt ggcaaaacac ttcttcaaaa
     2041 gaatatactc atttaagtcc cggaacgtat actttcatgg taaaaagtag aaatttatct
     2101 ggcataaatg gaaaagaaac aacctatcgt tttacaatac ttccaaaatg gtatcaaact
     2161 aatttcgctt tcattttata tattcttttt tctgtcctat ttatctattt catttcgttc
     2221 agaattcgga aaaagataga gaaagaaaga aaaaaagcac acttagaagc tgaaaaaact
     2281 aaaaagcttt tagagcttga attagagcag ctaaaactca ttcgagataa agaaaaaatt
     2341 aatgctgtaa aaatccactt ggaagaagat gtaatcgaaa aaagtaaaca attagctaat
     2401 tacacattat tattaagtca gaaaaaagaa ttatttaatg atcttcaaaa tgacttaaaa
     2461 aatttaagag aaatcaataa aaatgatgaa acgagaaaaa aaataactca aatttttcaa
     2521 aaactacatc aaaataaaat tggggaagaa tatatggaaa tcttcgatgt gaactttgaa
     2581 aaagtaaatc ataacttttt tgaaaaacta aaagaaataa acccaacatt aacaaaaaga
     2641 gaactccgtt tatgtgcttt tgtgaaaatg gatttaagta ataaagaaat tgctcctttg
     2701 cttaatattt caattagagg tgtagagaat gcccgatata gggctagaaa aaaactaaat
     2761 gtacaacatg aagaaaattt cgtatcctac ttaattagcc taattaatga ataaaaaaac
     2821 gcatttcaac ttgaaatgcg ttttaaatta aatataatta tattttagaa actcgccttc
     2881 aaatattctc tgtttaaacg agcaatattt tctaaagaaa ttcctttagg acattcaatt
     2941 tcacaagctc ctgtgtttgt acagtttcca aaaccttctt cgtccatttg acgcaccatg
     3001 tttaatactc gctcagtagc ttcaactctt ccttgtggca ataaagcata ttgagatact
     3061 tttgcaccca caaacaacat cgctgaaccg tttttacaag tagctacaca agccccacat
     3121 ccaatacaag ccgctgccat aaaagcatta tcagcatcgt gttttggaat tggaatggaa
     3181 ttcgcatcaa tcgtatttcc ggaagtattt acagaaacaa aaccacctgc ttgttgaata
     3241 cgttcaaaag cagttctatc aactactaaa tcttttatta ccgggaacgc tttacttctc
     3301 catggttcta caaagattgt atcaccatcg ttaaacattc tcatgtgtaa ttgacaagtc
     3361 gtgattccgg tatccggacc gtgagctctt ccgttaatgt ataatgaaca tgctccgcaa
     3421 attccttcgc gacaatcatg gtcaaaagca acgggttctt ttttatcgtt gactaattgt
     3481 tcgtttaatt gatctaacat ttctaaaaac gaactggctg ttgaaacatt atctaattta
     3541 tatgtttcaa ttcccccttt atcactggca tctttttgac gccatatttt aagggtaata
     3601 tttatatttt ttgctgcact catagtttct tatttgtagt ttctagctgc aatttttatt
     3661 tcttcgtaat tcaattcttc tttatgtagt tctgcttttg aaatatcagc tcctttgtat
     3721 tcccaagcac caacaaattt aaagttcaca tcatcccgta atgtttcacc ttcagcatct
     3781 tggtattctt ctctaaagtg acctccacaa gattcattac gttgaagagc atccatggcc
     3841 attaattgtc ccaattccat aaagtcagca acacgcagag ctttttctaa ttccggattt
     3901 aaatcatttg cacttccggg aataaaaaca tctttgtaaa attcttcttt taattgagca
     3961 atttctgtta tagcgtcttt taagccttgt tcatttcttc ccatcccaac tttgttccac
     4021 ataatcaatc ctaatctttt atggaaatga tccacagttt tagaaccgtt attgctcaag
     4081 aacttgtcga tagcagattt tacattattt tcagcttccg caaattcagg taaatcggta
     4141 gaaatttttc cggttctgat ttcgtctgct aaataattag aaacggtata gggcaataca
     4201 aaataaccat ctgctaaccc ttgcattaaa gccgaagctc ctaagcgatt tgcaccatgg
     4261 tcggaaaaat tagcttctcc agccacaaaa caaccaggaa ttgtggattg taaattataa
     4321 tcaacccaaa caccacccat ggtataatgt accgccggat atattttcat cggtgtttca
     4381 taaggattct catcggtaat tttttggtac atagtaaata aattaccata tttttcttcc
     4441 aaccactttt tacctaattt gattatttct tcttcagtag gattatgatt tccttgcccg
     4501 taagcagctt gttttccttt gcttttgatt tctgttgaga aatccaaata aacaccttcg
     4561 ttggtgtcat tggcttcaat accgtgtcct gcatcacatc gctccttggc agctcttgaa
     4621 gctacatcac gaggaactaa gtttccaaaa gcaggatatc ttctttccaa gaaataatct
     4681 ctgtcttgtt cctctaattg tgttggtttt aattttccgg cacgaatggc ttcagcgtct
     4741 tcttttttct ttggaaccca aattcttccg gaatttctta acgattctga cattaaagtc
     4801 aatttcgatt gattacttcc atgaacaggg atacaagttg ggtgaatttg tacaaaacaa
     4861 ggatttgcaa ataacgctcc ttgtttgtga attttccagc tggctgttac attacatccc
     4921 attgcattgg tggatagaaa atagacattt ccgtaacctc ccgtggcaat aataacggca
     4981 tgagctgaat gtctttccaa ctctccggtt attaaatttc tggcaataat tcctcgggct
     5041 ttaccatcta cttttactaa ttccaacatt tcatgacggt tatgcatttt cacacgccct
     5101 aatcctattt gtctggataa tgctgaatag gcacccaaca acaattgctg tccggtttgt
     5161 cctgcagcat aaaaagttcg ttgtacttgt gttcctccaa aagaacgatt atctagataa
     5221 ccaccatatt ctctcgcgaa gggaacacct tgagccacac attgatcaat gatgcttcca
     5281 gacacttcgg ctaaacgatg cacgtttgct tcacgggctc tataatcacc ccctttaatt
     5341 gtatcataga aaagacgata aacactgtct ccgtcatttt gataattttt tgcggcattg
     5401 atacctcctt gagcggcaat tgaatgtgct ctacgtggag aatcttggta acaaaaagca
     5461 ctcacattat aacccatttc gccaaaagag gcagcggctg aagcaccggc taaaccagtt
     5521 cctacgacaa tgatgtcaat ttttggacga ttattaggag caactaattt taaatgattt
     5581 ttatgatttg tccacttttg agcaactgga ccttccggta tttttgaatc taacttcata
     5641 atatattctg atgttgatta tttactaaaa tgaatgtaga gtggaataat tgcaaataac
     5701 aatggcacta tcactgcaaa agctttactc acaaacttga tggtgggtgt ccattttgga
     5761 ctatttaatc ctaaagattg gaacgcactt tgaaaaccat gccacaaatg aaatcctaaa
     5821 accaacatgg aaaacacata aaacaaagta aaatacaatc cgtatttttt atctttaaaa
     5881 aaatcgatcg tgattaaatg caaatcttta tacaaatctg caattttcac attaacctgt
     5941 gcattgtata attccgtttt gtttttaata attaactgct tttgaggatt gttttcaaca
     6001 gattctccct cttttaaaac ttgatcaact tgataatact gaccatcatt ttttccaaca
     6061 taaaaaacgt tttttacccc tgggctaact tctatgacat gtgtcataag aggcattttt
     6121 tcatcaaaat gcattctagc ccaaaaattt accatgtgcg tagcaatgaa aactaaaatc
     6181 aaagtcccta aaacagccat gcttcgagaa ggtaaactgc tatttgcacc ggcgtctgat
     6241 tttgcataac caattggtct tgcttttttg ttttgaacag ttaagatgat tccgtcaatt
     6301 gcatggaaaa gaattgaaat gtaagtgacg taagaaagaa tttttacgat tggattagag
     6361 gtcataaaca atgcatactg attgaattgc aatcgactgt cgggcattaa taattgtaaa
     6421 ttcccagcca agtgaccaac taaaaacaag cataaaaata aacccgtaag agccatccaa
     6481 tactttttag cgatagatga ctttaaaagt gcagattttg ccatagtgtt tgtgttaact
     6541 taatttttaa aaaaatagta tacaaattta atggtatttg aagacaacta caaccttttc
     6601 tgtctaattt aaagtgattt taaagaagga ttaacaagtg tttgacgtac tagttgttta
     6661 attataaaaa acgaaggata ttatttaaca aaactcggaa ttagacctct taaacccata
     6721 tcaacaacat tctcaccaat ggcaggttca aatttatcgt ctgttgttac ttctgtccat
     6781 tcaaaactag catttgtaga gagtgataaa gtaacctcta tgtctttagt ttcatttccg
     6841 gtaatggtta aatccgtttc aaatttacct gttacaacac aagatccttg aggaattggt
     6901 gaagtttgaa atagcggatt cggaaccgtt gtggctccgg caggtgcttg acctgaagtt
     6961 gcaaaaggca aactattcaa tgcaaaagcc caatatcctt gttctctatt gccgttcacg
     7021 ggaaacacat tattgccaat actatgggtg ccaatgtaag tgttatatcc aacaaaactc
     7081 tccaatgtac ctgtgtaagt tgacccttga ctgtaaactt gaatattccc gctttgatag
     7141 gataaagaaa ctcgaatcca gttgtaggct ccggctaatt gtggcgttat cggaattttt
     7201 aggaatactt cattttgtcc tacaatcttg gcttgactga aatctattgc cattgctcca
     7261 cctaaatcag tttcgggagc atgatacaaa acttttcctt gtcctaaaag agtattcacg
     7321 gaaggagaca actctatata atgagcactt attttttgca tatccggaga ctgagatcca
     7381 tttccgatag gaattaagga cggttgtcct aaattattta ggcgaacctg attttgatca
     7441 aatttgaatt taatgattaa attaggttgt ttggaagttt cattttcagt actgcatgaa
     7501 aaaataccga tgcttgccac aattagtagc aaaagtccgg taattttcca ttgtaatttc
     7561 attttaatac gttttagtta catcttcgtc tataacgagt atgaagtcag ctttattgta
     7621 ttcttccttt gctaatttag caatgtcttt ttgttcaact gttgcaatag tgattatttt
     7681 caattccggt ttatactttt tgagatacca atttattcct tcaaaattat cactatggta
     7741 cgttccgtta taatgaataa aaagggttcc aagttgcaaa ttttgaacaa taaaatgagc
     7801 catcgtagca tctttaattg cttgagcctt tggcatttta tctccggcat gtccgccttg
     7861 catcgacatc atgttttggt aaccgggtaa attaatatca aataaaatag gaagtggagc
     7921 tatccaattt ttttcttcag cactaagttt ctctaattcc tccaacccgt ttttaaaaac
     7981 catactggcg tatcgtcgtg gtatatttgt ggctacaaaa gggaattttt tttcttttgc
     8041 aaaatctact aaaggtttat aatccgtttt ataatttttc catagtcggg ctgttgaatc
     8101 aaattgcttt tgggtaattt cacctttcaa gtattggttt aattgtttct gattgtcggc
     8161 ttccaacatt tctgctccta ataccaactt cattttttct gctaaatcct tagtaagttc
     8221 taattgtagc caatgtacca cagaattgtc atggtattcg ccaaacaaaa ccacatctgc
     8281 tgtaactgcc tctttaaaaa gttttttata ggttgttttt tttccttctt ttgtaaataa
     8341 ctgatacgct attttgtttt gagcccatcc aatagtttga aaaacaagga aaaagcagag
     8401 tgataaatta atttttttca tttcttatga tatttaatgg tgtagattga gaataacttt
     8461 tttgagtcat tttgatttga tattttccgg aaaccaaata ggttttacca tttttagcct
     8521 ctttcaaaat aagtttttta tcttttttat gccatttttc agctcctgat tttgaaattt
     8581 ccaccatata agtccattga ttaattccgg aagttgccat ttgtttttct tcaaacacaa
     8641 caattccaat ttcattaatg acttgtacca acacttcacc ttggtttttt gtataataat
     8701 ccacaactaa tttaggttca tttggattga accatttgct ccaactactt ccccaattta
     8761 atgaataata aatttcgtca caggcaaaaa ttttcaaggc agaattgatt gtttcttcat
     8821 ttagttgctg tacagatttt aaattaactt tataaattga acgcccatga gttcctacaa
     8881 ttaaatcttt tgcttctgtt tgaatcacta aatcatgaac tgcgaccgga ggcaaaccgt
     8941 tcataaaagg ctgccagtta tcaccccgat tgaaagaaat aaaaaggcca ttatcagtac
     9001 caacatacaa aatggatgaa ttcttatcat cttctattat aacattaaca gcagctgttg
     9061 gaatatttgt cgcaattgac ttccatgttt gcccgtaatc ttccgacaca taaatatagg
     9121 gcgtgaaatc atcagaacga tagccgttta atgttacaaa aacacgctct ttttggtgtc
     9181 gagaagcaac cactctggaa acccaaagat cttttggaaa agaattggat attttctgcc
     9241 aacttgctcc tgcatccttt gatacatgga ctaaaccatc atcactaccc gtataaagta
     9301 atccaaactg aaacggactt tcactcaagg ttgaaattgt tccataaggt acattcccgt
     9361 ctttaccccc ttgtgtcaaa tccggagaaa tgactgacca agtatctcct tgattaaagg
     9421 aacggtgcaa atagtgtgat cccaaataca aaatatcttg attatgggat gaaagcaaaa
     9481 taggtgtttg ccaattgaat cgcaatggtt tttcctcttt tttggctttt ggagcaatgt
     9541 attttcgact ttcattactt ctatctatgc gatagtaatt tccaaattga aatccggtaa
     9601 aaacgatgga tggatttcta ttgtctattt gtgtttgcat tccatcacca cctaaaatac
     9661 tttcatacgg atatctacct tcttgatgcc attcgacact atactcataa ttattctgtc
     9721 ctacccaaac cccattgtct tgcatcccac cataaatatt gtatggcttt tggtaatcca
     9781 catttacatg ataaaactga ctaacaccat gaggattaca tttaatccaa ttggctccat
     9841 catcataaga aatattcaag cctccatcat ttccattaat taaatggcct gcttttttcg
     9901 ggtttaccca tacaatatgg tgatcggcat gtacattttc tctattaatc gacacaaacg
     9961 ttttccctcc atcatccgat ttaatgattg gcacacccga aacgtacaac ttatcagaat
    10021 tagacaaatc aaccgcaatt cgtccaaaat aatatccata agaataataa aaatcgtcta
    10081 tatagtctga atgggttttc ttccatgttt tccctccatc aatagattta taaacttctg
    10141 ctccaatcac ttctgtttca aataaggccg cgttcgcatc ttgcattatt ttttttgctt
    10201 ccgcaggttc catgtttccg ttttgaatcc aatttttaat attctccgcc ctgtattttt
    10261 cagaaagtcc ttgttttttc aaatatgcat ttatttcact gtttggaata ttcataaaaa
    10321 cttcaccggg agcactaaac atttcaggca ttgttgtttt tgtttggatt ttctttggtc
    10381 gttttgattg attatccaaa acggcataaa caatagcatc atcaaatgtt gctaaaccaa
    10441 ttctgcccac tccttcaccg gttggaaatc cgctttcagt tgtggaaatc aatttccaag
    10501 tagaaccacc gtcttcactt ttataaatgc cggaagtagt tccgttgcct ataaaattcc
    10561 atgctttacg gtcttttttc caagctgagg caaattgtat attgaagttt ttggatgata
    10621 cagcaatatc gataataccg gtttcatcat caatgaataa ggtttgcttc catgtctttc
    10681 cgccatcatt agttttgaaa acaccccgtt ccatgttttt tgaatacaaa tgtccggtta
    10741 cgcccacaac aacttcattc ggattagttg ggttaatttc tatttttcct atgtggtggc
    10801 tgtctttcaa acccatattt tgccaagttt ttcctttatc agtggatttc aaaattccta
    10861 ttccggcata agaagatcgc gaggaattat gctctcctgt tcctacccaa atggtactgc
    10921 tcttccaatc aactgctatt tcccccacat tttgagttgc cgaattatcc atgattggag
    10981 taaaactcat tccgttgtta ttggaatacc aaactccacc ggaagcatag cccacataaa
    11041 attcaattgg attatccgga tttacgtcga catcaaccac acgtccactc atgatcgtag
    11101 gtccaacact attaaatgct atgttttgaa caagtgaatt attttgtgac caactttgat
    11161 taacaagtag aaaaaaaaca aaagataaaa aaccattctt aatcatgaga ataaatttaa
    11221 aatggaaatt taggcattac ttaagtgttg acaaaatttt atacttgaat taacagcaaa
    11281 tcaatgtaat cttgttattt ttgataaatc ataataaaca acctttatat catgaagtac
    11341 cattcaatag accgcaattt atatatcaaa aaccgcacaa agtttacctc tcaaatgaaa
    11401 ccaaacagtg tagcgatttt taattctaat gataattatc cggtgagtgc cgatacgact
    11461 ttaccttttg cacaacaccg tgatatttta tatttatccg gagttgatca ggaagaaagt
    11521 atattgcttt tatttccgga tgcaccttat gaaaacttaa aagaggtttt gtttttaaga
    11581 gaaacaaatg aacatattgc tgtttgggaa ggagaaaaac taacaaaaga aagagctttt
    11641 gaagtttcgg gcatcaaaaa cgtgatttgg gttcaggatt ttcataaaac gttgaaagaa
    11701 gtgatgtctt atgctgatac gatgtatatc aacactaacg agcattatcg tgcttctgtt
    11761 gaaacagaaa cccgtgaggc acgattcatc aaatggtgga aggaaaacta tccggctcat
    11821 aaagtagaaa aaagcaaccc aattttacaa cgcttacgtt ctataaaaga aagtgaagaa
    11881 atcgatttaa ttcaaaaagc ttgtgatatt accgaaaaag ggttccgaag agtactttcg
    11941 tttataaagc caaatgtaat ggagtatgaa attgaagctg aatttgctca tgaatttttg
    12001 cgtaatcgct caaaaggatt tgcttacact cctataattg cttcaggaaa caatgcgaat
    12061 gtgttgcatt atattgaaaa caaccaacaa tgcaaagccg gagatttaat cttattggat
    12121 gttggtgctg aatatgccaa ttattccagt gacatgacta gaatgattcc ggtttcaggc
    12181 cgatttacag caaggcaaaa agaagtttac aatgccgttt tacgagtaaa aaatgaagca
    12241 actaaaatgc tggttcccgg aacattatgg aaacaatatc atgtggaagt aggcaaaata
    12301 atgacttcag aattgttgaa tttgggtctt ttagacaaag ccgatgtgca aaatgaaaat
    12361 ccagattggc cggcttataa aaaatacttc atgcacggca catcccatca tttaggatta
    12421 gatacgcacg attatgggtt attacatgaa cctatgcagg ctaatatggt ttttacggtt
    12481 gaaccgggaa tttatattcc aaaagaaggt tttggcatcc gaatagaaga tgatatggtg
    12541 attcaaaatg aaggagaacc ttttaatttg atgcggaata tccctattga agtggaggaa
    12601 attgagtcta taatgaatgc ttaattttag tttaaaggtt taagtattga aaagtttata
    12661 agacgacttg caggaatgta ggtcgttttt ttaaaattta tatgaaatca atccactaca
    12721 aaaacaccaa aatcaactac accgacaccg gtaaaggaac tgccattgtt ttacttcatg
    12781 gctttttaga aaatttaagc atgtggaaaa actacatcgc cgatttttca aaaaaaaacc
    12841 gtgtaataat tatcgattta ttgggtcatg gtaattctga atgcttaggc tatattcata
    12901 ctatggaaga ccaagcggat atggtacatg aagttttaca tgaacttaaa atccgaaaag
    12961 cagtatttat tggccattca atgggtggat atgtagcact tgcatttgct gagttatatc
    13021 cggatgtaat gaaaggattg gttttgctaa attcgacatc aagatcagac agtgcggaac
    13081 gaaaattgaa tcgcgaaagg gcaattttag ctgtcaaaca aaattatact gcttttgtta
    13141 gaatgtcaat tgctaattta tttaacgaag acaaccggga acgtttagca gtcgaaattg
    13201 agctagtaaa aatggaagct ttaaaaactc ccttacaagg aattgtcgcc gctttagaag
    13261 ggatgaaaat aagaaaagac agagaagttt tattgcattt ttcaccctat ccgatgatgt
    13321 taattttagg aaaaaaagat ggtgtgttga attatgagga aagtcttgac caaattaaag
    13381 attcaaaagt ggaattagtt acttttccgg atggtcatat gagttatatt gaaaatgaaa
    13441 cagaattaaa aatggttttg ctcaactttt tgaaaaaaat ataattgatt tttaccatat
    13501 aagcttatac cagagaaact tatatggtta taaaattttc tcctcaaaag gtatttctac
    13561 atttaaaatt tcatttaaca gctcaattaa ttccgtttta aaatcttcta aaacagaatc
    13621 atctacttgg tttaaaatta cttctcgacc ttcctttatt tgaaaaggca taaatcctgc
    13681 ttttaaattt ttaaaggaaa taattcctac ttccatttca gtattaggaa attccgcttg
    13741 aaccataaag gcataacaca acaattgaat aattttatca tgtttgatgt cggctgtcaa
    13801 accattccag ttttgaatgg ctaatccgtt ttgttctact ttaccggttt tgtaatcaat
    13861 aatacgaagt ttcccgtttc tgagctctat tctgtcgaca ttacctttga ttttgattgg
    13921 aaaaggtaag cgaacatctt gtaaaactgc ttcgaaattt ttttccaaaa acaacacttt
    13981 tactgcgtca tcttgctcca aaagccgttt ttctagttgt aaaaaattgt acacatttcg
    14041 tttggcaact tcaaaagcta ataggttttt acctttggta atttctcctt ctttataaac
    14101 agttttaaat tgtttgaaaa cttcactatc agcaatttta agcatatttt caacgtttga
    14161 gatacttaaa aatgtattca tataaggttt atacaaaact tctaaagctg catgaataat
    14221 tgttccgagg gtattaatgg caatattttc ttcgacttct tccgtttcag aaattcgcaa
    14281 aatccgttga aaataaaact gcatcggatt tcgaatataa gttgtcaaag ttgaaggaga
    14341 aaatccgtta tcggccattt cacgcaatct ttccatcacc aattcagatt tttctacaac
    14401 cattggttca tgagctttct cgggtaaaat tgcattataa atttggtgcg aaagaacatg
    14461 attaggctgt ttttcaactt ctaattgggt tataaaccta cttttttctc cggcatcaat
    14521 tccttctgat tcagtattat aaagcaaata gatgtttttt gcccgttgta aaagatgata
    14581 aaagtgatag gtgtaaatgg catctttttc tttgaaagtt ggcaatccta attctctttt
    14641 cacatctaaa gggatgaatg aattctgcga tttcccggct ggaaagacac cttcattcat
    14701 ggaagtgatg attacagttt cgaagtctaa aacacgactt tccaatacac ccataatttg
    14761 aaggccttgc aatggctctc cttcaaacga aacttctgct aaatcaataa tttgtttgta
    14821 taaaccatgt agcccttcaa ttgaagtaat atagttgtgt tctgaacaat aagtactcag
    14881 tttattaatt atcttaaaga tggcataaac aaagctattg gtgattttat cctttgaatc
    14941 ttccgattga agaaattgtt taatttgaag caaaacggtt gctaaagtat caagtatctg
    15001 gaccgtatta gctggccact tttcgaataa aagttgaaaa aatgaatttg actgattgta
    15061 catttcaacc aactttacat gagaaatgaa cgtataatta tattgattaa tttgatttac
    15121 aagtgttttt gcatttacaa aaggttcaac taatggattt tgaaggactt ctaacacatc
    15181 tttataataa aacacatagc tagattgact tcgtttgaaa gcattctgat gcattttgaa
    15241 caattttgaa agtaacaatt gtgccggatt attctttccg gaataaccca ttgtaatgtt
    15301 taggtgcgaa acagaattgg gtaatgaatt taaaagcgga attaaaatat tttcttcccc
    15361 taaaacaatg gctacttttt ctaaaggttg ctggtcagca atggtttgaa ttatatcagc
    15421 tgctaattta gcttgtccaa ttgttttggg cgtgcccatt atttggatat tttttggctc
    15481 tttaaattca ttaaaaatcc attgataagg attgttttta taaaatggcc aactcttttt
    15541 tattttacgg gcaaacaaac cggcatcatg ataagggtca ttcaaaaagg tttcttcagc
    15601 atcccataaa acagtagctt tttcaataga taataaatgc tgaaaaatca tttcttctgc
    15661 tttatttaaa gcattgaatc ctgcaaaaat aaaatgatga tttcctattg tgttggagaa
    15721 atggtttaaa ttttctactg cttcacgata aattaaccct tgatatccaa ttcctttgtt
    15781 taataaatgc ttataaaaag agtcataata caaaggcata ttgtcccaaa attggagata
    15841 attttcaatt aatcttgttt tatcctgtaa ctcaagattc catcttttta tggcttcaat
    15901 ttctttcaga taagaaaaaa catgatttgg agagagaaga aatcggtcta tttcattaaa
    15961 atcttgaagc aacgttttcc cccaattaga aaattgttca aacgattgtt gttcattttt
    16021 atttgttacg gaaaggtaaa ccgaataaaa ctcaaataat aattcaattg aatctatagt
    16081 tctaatagtt gctatctctt gaataaaatc ttcaatgtta ataattctag gggcaaagct
    16141 attggattga atagtttgtt ttaattcttc taataaaaaa acccgagctc ttttattagg
    16201 aagcacaata gttaagcttt ccatttcaaa agaatggtta gtcaaaatat gattagccaa
    16261 taaagataaa aaagagtttg ccttcatttt ataaaaataa aaaaaacgtc ccgataaatc
    16321 gggacgtttt cagtatttat tttgaaagaa attatttgat taatttcact tcaactcttc
    16381 tgttgtttgc tttacccatt ttagttttgt tggtatcaat tggcattgat tcaccaaaac
    16441 ctttagattc taatctagaa gcgttaattc cgttttcaat taaataattt ttaacggctg
    16501 cagctctatc ttcagataat ttttggtttg aagcatcttt accatcgcta tctgtatgac
    16561 cttcaattga gaatctagca tttggatatt cttttaagat accagtgatg ttttgtaata
    16621 ctgtaaatgt tctttgttgg aaagttgctt ttcctgattc aaacaatatt gttttagcat
    16681 aatctcttaa tgagctaaga attgcttcgc taatttctgg acaaccattg ttagcaactg
    16741 ttccagctac atctggacat ctgtcatctt tgtcaaatac tttatctcca tctctatctg
    16801 gccatggaca accttggttt tcttttggac cttttacatc tggacattta tcatctttat
    16861 ctgcaattcc gtcaccgtct gtatcaggac atccgtttaa ttctttagga ccttttactt
    16921 ctggacaaga atcatcttta tcagcaattc cgtctccgtc agtatcagga caaccttgga
    16981 attcagctaa accaaataca tctggacaag catcactacc atcaataata ccatctaagt
    17041 cagtatcagg acatccattg tattgtttta aaccaaacac ttctggacaa gcatcatctt
    17101 tgtcataaat accatctccg tcagtatctt tacctccgaa tttgaaagta acaccggcaa
    17161 aatgttgcat gtgagttggc ataactgatc tatcgtcttc aaatgaatgt ttgtatgttg
    17221 aacgtaatcc aagaccaatt gtttctgata accaaatatt taaacctaaa cctccgttta
    17281 cagttccggc actgttatca cctaagaaag tgtaacctcc accaacgtgt acagtaggct
    17341 caaaagcttt aagaaatttt ccaatactat aattaactac agcatcaaca ccgtaataca
    17401 ttaagtcaac atctgcaacc acatattctc catctactct aggttgaaca aatttttcaa
    17461 gtctattaaa agaccctgtt aacccaaatg aaaaattatc tcctacgtat ctggaaacag
    17521 aaaggtaaga aactgaaggt acaacactcc aataatcttt agcgttaaaa tattccgaaa
    17581 actgatctgc aacactacca gcagcactta ctcggccacc gtctaatgca ttaaccccaa
    17641 aggtgaaagc ccaaggatta tcactatctt gtgcttgcga actaagtccg gcaaacataa
    17701 agatagctaa aactaatttg ttaagatgtt tcatacttga tttgtttttt tatttacaat
    17761 atattgttaa ttagtgcaaa aatacggtgt tttaaattaa ctacaaaatt taatgttaaa
    17821 aaaattaata agaatactaa actaaaacta tataatattc aatatttttc cgacttcagt
    17881 gaaagcttta atggctttgt caaggtgttc ttttgaatgt gcagcagaca actgaactct
    17941 tattcttgct ttatctttag gcactacagg gaaaaagaac ccaattacat aaatcccttt
    18001 tttcaataat tcatttgcca tcacttgtga taatttggca tcatatagca taaccggaac
    18061 aatcgctgaa tcaccatcaa taatatcaaa cccggcatct ttcattcctt ttttgaaata
    18121 attggtattc cattctaatt tatcgcgtaa agcggtgtct ttctctaaca actcaaacac
    18181 ttttaaagaa gctccaacaa tacttggagc taaagaatta gaaaataaat agggtctaga
    18241 tcgttgtcta agaatttcta taatttcttt tttggcagtg gtgtaacctc ccatcgctcc
    18301 acctaatgct tttcctaatg tcccggtaat tatatctact ctgcccatta ctccttttgc
    18361 ttccatcgtc ccttttccgg tagctccgat aaatccagca gcatgacatt cgtccaccat
    18421 gaccatggca tcgtatttgt ctgctaaatc gcaaatttta tctaatgggg caaccaatcc
    18481 atccattgaa aaaacaccat cagtcacaat taatttgaac ctacgtcctt cttcatttgc
    18541 tttaattagt tgttgttcca aatcagccat gttgctgttt tcataacgat aacgagctgc
    18601 tttacacaaa cgaacaccat caataatgga tgcatgattt aaactgtctg aaataatagc
    18661 atcttcctca ttaaataaag gctcaaaaac accaccattt gcatcgaatg ctgcagcata
    18721 taaaatggtg tcttctgtgc cataaaaatg ggcaattgtt ttttctaatt gtttgtgaat
    18781 atcttgagta ccacaaatga accgaacaga agacattcca aaaccatgtg aatccaatgc
    18841 gtcttttgcg gcttgaatta cttcaggatg agaagataat cctagataat tatttgcaca
    18901 aaaatttaaa acggtttccc cggttgaaat ggtaatttct gctccttgag gtgatgtaat
    18961 aattcgctcc tttttaaata tcccattatc ttcaatggtt tttagctcgt tttgtaagtg
    19021 ctgttgtatt ttaccgtaca tattgttttt gttttttaca aagttactat ttccaatgtt
    19081 tctccaatat aaactaatgc tttttttata actttaaacc ccatgttgtg tagtgctgtt
    19141 tcgtatattt ctaattgttt ttgatgtttt aagtttggtt cgccagtttt ataatccaat
    19201 aacatggctt gattttgttg gttaaataca attctatccg gtttaataat agaatcggat
    19261 tttctcaaaa tagtttgttc attcagcgtt ttattagttt catcaaaaaa taaattcaat
    19321 tcaggatgcg agcaaacttg aatgattatt ctcttcactt cttctttttg tgaaaccgaa
    19381 attaatccgc cttccaaagc tttggaaata gcccattcaa tatcatttct ggttttgata
    19441 taagacaata tttcatgaat tacattgcca tattcaatgg ctttttgttg atgtgtaccc
    19501 cacattaaac tctcccgttg tgcaattttg atagattcag agtttaatac ttcttggact
    19561 aacggtattg gttttaattc ttgcgttttt tttcctgaag aagaaattct gctttcagaa
    19621 ccccattcat aagtagtttt atgcctatct aacaatcctt tctcttctaa aaatttcaga
    19681 aaaaaggaag acatgttgtt ttgggaaatt tcacctttcg atgttaaatt cataccggaa
    19741 ataatatata attgttcctc agctcttgtc attgcaacat ataaaatatt gatattgtct
    19801 aataattctt cttccttttt ttgatgaaaa acctgagcag cagaatcacc tatttcttct
    19861 acgcttttat tattgtccac taatatttta ggcaaagaaa tcaaatcaga ttccggttct
    19921 atccataatt tatcttttgg tgattttgaa taattctctt ctgcatatgg aaatagtaca
    19981 accggaaatt ccaatccctt ggctttatga atagtcatga ttcgaatggc attgttgcct
    20041 tccggtgaag gaatactgaa acgatgtgcg ttctcctccc aataaagcaa aaaatctgaa
    20101 atgccggact gatttttgat gtctcggtct aaaacccggt ctaaaaaatc ttgaatatag
    20161 gcgttagttt gttttggttg aatgaacgtt tttatacaac tttcaactac ttcatacaat
    20221 gatttttttc gtaatttttg aaaggagaag attaattgaa tagaagccaa ccagttttct
    20281 aatgccgatt ctgttataaa actcatgccc attgcaatga aatcatgaac cgagctgtta
    20341 ctttgccttt tagttccgat ataatacaaa aaatgagctt ttgcctcttt atcttctcca
    20401 tttttcaaaa acctcaacaa ggctattata aaactaactt ccgaagagtt ttgtagcaaa
    20461 agtgtttcag aagacacaat tgggatactg ttttccgtta aaaaagtagc tatttttaca
    20521 cccggttctt tttttcgggt taaaataacc atatcgcgga aagcgaagtt tttagattgc
    20581 actttctgaa tggtttgaag tattgcctct aaataaagtt cgtctttttc aacatcttcc
    20641 tcccagtcag attttgatgg aataaaagaa agatttacat aaccgccttt tttggaattt
    20701 tctttttgat gactatgatt ttcatataaa tctttataat caggatattg gaattcattc
    20761 gatagaaatt ggaaaaactg attattaaaa tcaatcactt ctgaataact tctccaattg
    20821 gtatctaaat gaaaaagttt tttatccgga tttacaaacg gatttttggt tttactcaat
    20881 tcaataaact gttctgcttt tcctcctcgc cagcgataaa tagattgttt tggatcgcct
    20941 acaatcatga gcgaccctct ttcgcctcct aaatcttcac ttgacaaggc attgtcaatc
    21001 aatggaatca aattttccca ttgcatttga gaagtatctt gaaattcatc gataaaaaaa
    21061 tgcttgtaac gttcacccat tcgttcataa ataaaaggag ccggttgatt ttgaatttcg
    21121 ttatgaataa ttgcattgaa ctcggctatc gaaagaatat tttgttcgtc ctgaatttta
    21181 tgtaattctt gacttactcg attgagcaat gataacggag taatgttttt tagaaaagcg
    21241 ttgtagagta
//
LOCUS       DAOTCW010000028        20464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1402_length_20464_cov_49.148562, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000028 DAOTCW010000000
VERSION     DAOTCW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 20464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20464
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1402_length_20464_cov_49.148562"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /gene="dinB"
                     /locus_tag="P5335_03880"
     CDS             complement(<1..1043)
                     /gene="dinB"
                     /locus_tag="P5335_03880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRZ74051.1"
                     /translation="MMEEINYRKIIHIDMDAFYASVEQHDFPELKGKPLAVGGSEIRG
                     VVSAASYEARKFGVRSAMSGVQAKKQCPDLIFVRPRFDRYTEISKIIRKIFYEFTDLV
                     EPLSLDEAYLDVTQNKKGNPSATLLAQEIRKRIFEETGLTASAGISINKFVAKVASDY
                     NKPNGQKTVNPDEVEAFLEALDIRKFYGIGKVTAERMYQLGIYSGKDLKTRSLAFLEQ
                     HFGKSGTHYFYIVRGIHNSPVKPNRISKSVGAERTFNENLSSEVFMEDRLESIAKELE
                     RRLQKHKIAGKTVTLKIKYSDFSQQTRSKTLPYFISDKALLLETAKELLYQEKLKESV
                     RLLGISLNNLNTEV"
     gene            complement(1018..>2469)
                     /locus_tag="P5335_03885"
     CDS             complement(1018..>2469)
                     /locus_tag="P5335_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HRZ74052.1"
                     /translation="PFAAFEKELLSIQDKIKTKDNNHFTTSESLSYKQLFVLHKQCEE
                     YVEKAFQNSSKFGITLRVNQSLLRIRQQLYRLKILIPLLVVDVETDKKKNSIRLAYKL
                     IKYNCTRNNVRTFIKESTQLLSYEITQHTAKTGEKYITQSRSEYFKMFNTALGGGLIV
                     GILCVIKVLLSKVETSDFGHAFLYSMNYSFGFIAIYVLGFTLATKQPAMTASALVKVL
                     EDEIKENGNSNQKHQGFAVFFARVFRSQFIAFVGNVIMAFPISLLGIWLIDLAFHYNI
                     AETKWNTLVTDLSPVHSMAIFHAAIAGCFLFLSGIISGSVANRDKHYQIYLRIKEHPF
                     LKRSFGKEKAERFAHWYEKKWAGIISNFWFGIFMGSTASIGVFFGLNLDIRHITFASG
                     NLALGLYGSNYTLDNWMLFWGIFGIGIIGLVNFMVSFGLSLGLAFRSRAIPLSEFKPI
                     SASIWRHFKSKPFSFFFPIGTIEVHHDGGDKLP"
     assembly_gap    2470..2569
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2570..2795)
                     /locus_tag="P5335_03890"
     CDS             complement(<2570..2795)
                     /locus_tag="P5335_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74053.1"
                     /translation="MAVSFTKKKKVTTIDVLTVYFDNNKRWETTIDPLEILVKIVASI
                     RPKNLKKTDEIDLTELLLFLKKNKSITNQFS"
     gene            2872..3342
                     /locus_tag="P5335_03895"
     CDS             2872..3342
                     /locus_tag="P5335_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HRZ74054.1"
                     /translation="MIEIERKFLVTSPQFKKEAFQESRIVQGYLNSHPERTVRIRIKG
                     NKAFITIKGIGNETGLTRLEWEKEISVADAEKLLKLCETGIIDKVRYDIKIGNHIVEV
                     DVFSGENEGLIMAEIELESEEEAFEKPYWLGEEVTNDERYYNAYLSRNPFKNWS"
     gene            complement(3344..3844)
                     /locus_tag="P5335_03900"
     CDS             complement(3344..3844)
                     /locus_tag="P5335_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRZ74055.1"
                     /translation="MKHKTELIVFGLFALLFFSSFSFETKHFEKPSLNRIIHSDTTKN
                     DKTAIDSIATEGNFTFKLYKKGAHASYYADKFTGKRTASGQVFDNQKHTAAHRKFPFG
                     TKVRVTNEANGKSTIVTINDRGPFTKGREIDLSKRAFQDIARHNGYGSMKVTIELVEE
                     IKDTIN"
     gene            complement(3926..4348)
                     /locus_tag="P5335_03905"
     CDS             complement(3926..4348)
                     /locus_tag="P5335_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74056.1"
                     /translation="MYELLQKLHSWWAYLALILLFLAVINAVLGYTGKKLFQPKDLRI
                     SLFALIFTHIQLLLGLILYIVSPLGLDSLGQMKDPAIRLTSMEHPLINIIAIILITIG
                     WSKHKKVANNLKFQKIMVFYGLGLLLILIRIPWSSWLG"
     gene            complement(4420..6063)
                     /gene="pgi"
                     /locus_tag="P5335_03910"
     CDS             complement(4420..6063)
                     /gene="pgi"
                     /locus_tag="P5335_03910"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRZ74057.1"
                     /translation="MALKSTNPSGTAAWQNLKKHFEEMQHVSMQELFAKDAKRAEKFH
                     IQWNDFLVDYSKNILTEETKQHLLALANEVDLKDAISKYFAGDLINQTENRAVLHTAL
                     RASTNASIMVDGVNVMPEVVAVKNKIKNFTEEVVNGERKGFTGKPFTDIVNIGIGGSD
                     LGPAMVVEALQFYKNQLNVHFVSNVDGDHVNEVIKKVNPETTLFVIVSKTFTTQETLS
                     NSETIRHWFLKSAKQEDVAKHFVAVSTNIQKVTEFGIHPDNIFPMWDWVGGRFSLWSA
                     VGLSISLAVGYENFDKLLIGAHDMDEHFRTTPFEKNIPVILALLSIWYNNFFGAESEA
                     LIPYTQYLQKLAPYLQQGIMESNGKSIDREGNPVNYQTGTIIWGEPGTNSQHAFFQLI
                     HQGTKLIPTDFIGFVHSLYGNQDHHDKLMSNFFAQTEALLNGKPAEQVMAEFEKQGVS
                     GEKAEFLLPFKIFSGNRPTNTFLITSLTPETLGSLVALYEHKIFVQGVIWNIFSYDQW
                     GVELGKQLANSILGEITSGKIKNHDSSTNFLLTHFLNQR"
     gene            complement(6074..>6616)
                     /locus_tag="P5335_03915"
     CDS             complement(6074..>6616)
                     /locus_tag="P5335_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRZ74058.1"
                     /translation="SKKVDYYDEEGKGLKTMFLKAPLDYFRISSRFSPKRFHPVQMTW
                     KAHKGTDYAAAQGTPIKTTASGVVEKTGYTAGNGNFVKVKHNGTYSTQYLHMSKILVR
                     QGQRVEQGQVIGKVGSTGLATGPHVCYRFWKNGVQVDPLSQKLPNTEPMNEKHRKNYL
                     TYIAPLKRELDSIATLQFKK"
     assembly_gap    6617..6716
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6717..7467)
                     /locus_tag="P5335_03920"
     CDS             complement(<6717..7467)
                     /locus_tag="P5335_03920"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HRZ74059.1"
                     /translation="MKFKIVTISLITLLLFACEEKDKKVVPKEVIKEEPIIVEFGFTY
                     NDYLVVQDTVQSGDTFSKLLEENNIGTFKVHDVTEMVKDSFNLRDIRIGKPFTLLKSK
                     KAPHQLQVFIYQKDNIHYSVVDFRDTVVVAYNKQKPITIKRRTIATSISGSLSETLSK
                     NGVDASMANNLAKIYEYSIDFFKIQKDDKFAVTFYEKYINDSIYAGVERMESSFFEHK
                     GKKIYAFPYKLDSLSKKVDYYDEEGKGLKTMF"
     gene            complement(7487..8422)
                     /locus_tag="P5335_03925"
     CDS             complement(7487..8422)
                     /locus_tag="P5335_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HRZ74060.1"
                     /translation="MNSTAHKEILEQIEQKFQAINQKTDTHLEGLLWAKPITYWDYIQ
                     TDALLNLQTQRTTLPDEMVFIMYHQVNELLFKMILWEIEQLCQTEQPETAYFTEKLMR
                     ISRYFDMLTTSFTIMKEGMDVEQYMKFRNTLTPASGFQSAQYRFIEFSSTDLINLIDY
                     RFRATIDRNTPFEYAFEHLYWQAAGKDYQTGEKSFLILEFERKYKKEFLRFMEEYNTT
                     NLWRKFKQLPENDQKNEALVNAMRHYDYTVNITWVMGHYHAAKKYIESGHGTGEATGG
                     SDWKKYMLPKYQRRIFFPELWTAEELATWGEKESI"
     gene            complement(8422..9216)
                     /locus_tag="P5335_03930"
     CDS             complement(8422..9216)
                     /locus_tag="P5335_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRZ74061.1"
                     /translation="MKKLFLFVIFFSTVSFDSSPTLQPKKEDAFEVGEWFKFRIHYGL
                     INAGYAELEVKEAVRNNKKVFHAKGRGYTVGMTKFFFEVEDDYQSYFDKESGNPYQFV
                     RKIDEGGYTKNQEGFFNHKSKTILVKDYKKKTEKTFEVPDNTQDILSTFYYLRNHPNV
                     DKLKVGESINVDMFFDDETTKFKLKFIGREDLKTKFGVISTMIFRPYVQAGRVFKEQE
                     SLTVWISDDDNKLPVRIKASLAVGSIKADLEQFKGLKHTFTVKAKP"
     gene            complement(9604..10764)
                     /gene="hppD"
                     /locus_tag="P5335_03935"
     CDS             complement(9604..10764)
                     /gene="hppD"
                     /locus_tag="P5335_03935"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRZ74062.1"
                     /translation="MAKDVKSVEYGLEKIFEGAQDFLPLLGTDYVEFYVGNAKQSAHY
                     YKTAFGYQSHAYRGLETGSKDSVSYVLKQDKIRLVLTTPLTSSSPINEHLAKHGDGVK
                     VIALWVEDARSAWEETTKRGAKSFMEPTVEKDEHGEVVRSGIHTYGETVHIFVERKNY
                     NGAFLPGFVEWKSEYNPEPLGLKYVDHMVGNVGWGEMNQWVKWYEDVMGFENFLSFDD
                     TQIHTEYSALMSKVMSNGNGRIKFPINEPAKGNKRSQIEEYIDFYEGSGVQHLAVATD
                     DIIKTVAGLKSRGVEFLPPPPQAYYDDIPRRLGVHMDMMKEDIKELQRLSILVDADED
                     GYLLQIFTKPVEDRPTLFYEIIQRMGAKGFGAGNFKALFESIEREQALRGTL"
     gene            complement(10833..11201)
                     /locus_tag="P5335_03940"
     CDS             complement(10833..11201)
                     /locus_tag="P5335_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015361987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRZ74063.1"
                     /translation="MIFTNSSKYQNNPILKESFEFALMVVKYSELLDENRKYVIAKQL
                     IRSGTSVGANIKEAQNAESKGDFIHKLKISLKEADETEYWLFLCENLENYPNPKELIE
                     KLNGIIKMLNKIISTSKNKV"
     gene            complement(11264..12418)
                     /locus_tag="P5335_03945"
     CDS             complement(11264..12418)
                     /locus_tag="P5335_03945"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRZ74064.1"
                     /translation="MPFYHKLGNIPPKRHTQFRKPNGDLYYEQLFGTVGFDGMSTNSY
                     HEQRPTQVKEIRSQYSIAPIIAKSNNIQSYKLSGFDVAPQDDYLESRKIVLTNSDCHI
                     VLAAPRKSTTDYFYKNTDSDEMIFVHKGSGKLRTIYGNLDFKYGDYLIVPRGIIYKID
                     FDTEDNRLFIVESHAPIYTPKQYRNWFGQLLEHSPYCERDIRRPYELETNNEKGDFII
                     KLKKKDEIFEMVYASHPFDVVGYDGYNYPYAFSIHDFEPITGRIHLPPPIHQTFETTA
                     FVVCSFVPRLYDYHPLAIPAPYNHSNIDADEVLYYVDGDFMSRNDIKQGNISLHPAGI
                     AHGPHPGAAERSIGKKETLELAVMVDTFKPLMVTEEAMKIADENYFQSWL"
     gene            complement(12712..14829)
                     /locus_tag="P5335_03950"
     CDS             complement(12712..14829)
                     /locus_tag="P5335_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRZ74065.1"
                     /translation="MLSGGGAKGLAHIGVLKKIDEAGIKIDYIGGTSMGAVVGGLYAS
                     GYTGIQIDSIFKAADYDAIIQDYTPRVSKDYYERENNDRYALTLPFNKFKLGVPIALS
                     RGLYNYNLMTRLMDHVRHERNFKKLPIPFVCMATDIETGQEVLLDHGFLSQAIVASGA
                     LPTLYAPVIIDGRFLIDGGVLNNFPVEEVKNMGADIIIGVDVQYELKEREELTEATKL
                     MVQISNLQMIKKMEEKKHLIDIYIKPSINEFTVVSFDKGPEIIKIGEDVAMEYMEAFK
                     KLSTNYKRPTTVFPKNETDSIYISSIKINPLENYTKYYIQSKLRFKPGQKISYADLVR
                     GIDYLNTTQNFSSLSYTLEKEEEGDQLNLFLVENPMKTYLKLGLHYDGLFKSGILVNY
                     TKKKLLFKNDVFSADVVLGDNFRYFLDYNVDIGYNLSVGLNSKFYQFNRNVNTNFSAW
                     DVLREAGTNSINIDYRDFTNQLYLQTLFVQKFSAKIGVELMNLKIQSETLSNSTAIFD
                     NGDYLSAFGNMNFDSFDNKYFPKKGWYFNGDVQSFLYSSRHEDIFLMHTIFKGEAAYA
                     QKLFRKGSFVIKSEGGFTVGEQSIPILNFFMGGYGYKPFNNFRHFYGYDFVSLTGNSY
                     VMGSLSFDYEIFKKNHINVAANYANIGYDIFVNDGWLKLPTFSGYSVGYGLETLLGPV
                     EIKQSWSPETKDSFTWFGVGFWF"
     gene            complement(15013..16812)
                     /gene="uvrC"
                     /locus_tag="P5335_03955"
     CDS             complement(15013..16812)
                     /gene="uvrC"
                     /locus_tag="P5335_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRZ74066.1"
                     /translation="MNNPSLALQLQTLPENPGVYQYYDKEGKILYVGKAKNLKKRVTS
                     YFNKTHDNAKTNVLVRKIVEIKHIVVSTEQDALLLENNLIKKLQPRYNVLLRDDKTYP
                     WICIKKEPFSRIFSTRKMIKDGSEYFGPYTSFKTVHTLLDLIKELYPLRTCHYDLSEA
                     NIKSNKYKVCLEYHIGNCKGPCEGFELLENYQKQVDAIRQILKGNFKESLKEFKLKMH
                     ELAAAMQFEEAQKMKEKIGVLENYQAKSTILNPKISNIDVFSIVSDETMAYVNFLQIA
                     HGAIIRSYTLELKKKLDETDEELLELAVVELRERFHLTSKELILPFALDFGDKINVTV
                     PQVGDKKQILELSQRNAKYHRLEQLKQIQIVDPERHTNRIMAQMQKDLRLSVEPRHIE
                     CFDNSNIQGTNPVAACVVFKDGKASKKDYRHFNIKTVVGPDDFASMEEVVYRRYKRML
                     DENQLLPQLIIIDGGKGQLSSALKSLDDLGLRGKIAIIGIAKRLEELFYPGDTVPLYL
                     DKKSETLKIIQQLRNEAHRFGITHHRDKRSKSALETSIETIPGIGEKTMVTLIKHFKS
                     VKRIAQASENEISTVVGVAKAKKIYEFYKAINK"
     gene            16886..17452
                     /locus_tag="P5335_03960"
     CDS             16886..17452
                     /locus_tag="P5335_03960"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRZ74067.1"
                     /translation="MNKSDLRTRYKSLRAQLTAEEIEEKSLAIANHLLQVDIWNKTYY
                     HLFLSIVEQKEIDTEFILQILAGKDKEIVLAKSDFKTGEMTHFLLTDNTKIKKNNYNI
                     PEPVNGLEVPVSKIDVVFVPLLAFDKKGHRVGYGKGFYDRFLTACNPETLKIGLSFFE
                     AEESIEGTSANDIPLDYVVTPEKVYFFD"
     gene            complement(17449..18075)
                     /locus_tag="P5335_03965"
     CDS             complement(17449..18075)
                     /locus_tag="P5335_03965"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HRZ74068.1"
                     /translation="MTLKKAYLIVFLILLADQVSKLYIKTHFILGGEIKVFDWFRIHF
                     IENEGMAWGTEIPGDYGKLILTLFRIVAVFGIGYWLWDCVRKASSTYLIVAVSLILAG
                     AFGNIIDSVFYGVIFNDSYNAPAVLFSEQPYGTWFHGKVVDMLYFPIWKGTLPEWLPI
                     IGGKYFTFFNAIFNIADMAISTGVGILIVFNKKAFGTTDKEQPTEVAA"
     gene            18260..19504
                     /locus_tag="P5335_03970"
     CDS             18260..19504
                     /locus_tag="P5335_03970"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74069.1"
                     /translation="MKIVKFVAIAFVLSFFASCNNEDDNGEMAKFAVPEIKSLASIRS
                     SISVTPAQATNADGKVYVAENLLFYVAQESGIHIFDNSNPASPQNTAFINLEGVHDIA
                     VKGNFLYADNFIDLVVFDLSNLGNITVVQTVQNAFEFYPSYPEGAEFYAFDVYPTANE
                     IIVGYRLENRRRPEGQDLILANDALAGFESSAAGAVGTGGSYAKFQINNNALYTVESY
                     ELNVFNISDPTQTFFDKAVYMNEWLGGGQFETLFKQKEFLFIGSTNGMYIVNAQDEFN
                     PYFVSGFSHATACDPVVVFANTAYITVRGGSTCGAIEDQVNVIDITNIENPTLISTYL
                     LSDPRGLGIKDNVLYVCADGNLNVFNATNSAELTLQNTYSDGVQDVIALNSHLIAVGN
                     NKIIQYNYGENHSLEQISVVNF"
     gene            complement(19566..19841)
                     /locus_tag="P5335_03975"
     CDS             complement(19566..19841)
                     /locus_tag="P5335_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HRZ74070.1"
                     /translation="MDINFTPNSWEDIEYWIENDPEIITRIKDLIKSIRQDPFKGLGK
                     PEPLKYDLKGYWSRRITGEHRIVYKVLGTKGVDQRCIILQCRFHYDD"
     gene            complement(19844..20134)
                     /locus_tag="P5335_03980"
     CDS             complement(19844..20134)
                     /locus_tag="P5335_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRZ74071.1"
                     /translation="MKAVSISTLRKNIKNYFDEVTESSEMLVVSRNDEEGAVVIISLK
                     EYNSMTETQHLLSTKTNRKRLADALQQLENGELRTIQSGNDFLKDEFVTPSV"
BASE COUNT         6449 a         3737 c         3233 g         6845 t
ORIGIN      
        1 acttcagtat tcaaattgtt taatgaaatc cccaacaaac gaacggattc ttttagtttt
       61 tcctggtata ataattcttt agccgtttcg agcaataatg ctttatctga aataaaataa
      121 ggcaaggttt tgctacgggt ttgttgagaa aaatcagaat atttaatttt taaggtcact
      181 gttttaccgg caattttgtg tttttgcaaa cggcgttcaa gttctttagc aatactctct
      241 aaacgatctt ccatgaaaac ttcagaagac aggttttcat taaacgttcg ttctgctcca
      301 actgatttag aaatgcgatt cggttttacc ggactgttat gaatacctcg aacaatatag
      361 aaataatgtg tgccgctctt gccaaaatgc tgttctaaaa aggctaaact gcgggttttt
      421 aaatctttcc cggaataaat acccaattga tacattcgct ctgccgttac tttcccaatg
      481 ccgtaaaatt ttcgaatatc taatgcttcc aaaaaagctt ctacttcatc tggattaacc
      541 gttttttgcc cgtttggttt attataatct gaagctactt tcgcgacaaa tttattgata
      601 gaaatacccg ctgaggcggt taagcctgtt tcttcaaaaa tacgcttacg aatttcctga
      661 gctaacaagg tcgcactggg attccctttt ttattttgag taacatctaa ataggcttcg
      721 tctagtgata gcggttcaac caagtccgta aactcataaa atatttttcg gataatttta
      781 gaaatttcgg tatatcgatc aaatcgaggt ctaacaaaga ttaaatcggg acattgtttt
      841 ttggcttgca caccactcat cgcactgcgt acaccaaatt ttctggcctc ataactggca
      901 gcagaaacaa ctccacgaat ttcacttcct ccaacagcca aaggctttcc ttttaattcc
      961 ggaaaatcat gttgttctac agaagcgtaa aacgcatcca tgtcgatgtg aattatctta
     1021 cggtagttta tctcctccat catgatgcac ttctattgtt cctattggaa aaaagaaaga
     1081 aaatggtttg cttttaaaat gtctccaaat agaagcggaa attggtttaa attcagaaag
     1141 cggaatagct cttgatcgaa aagctaaacc aagagataag ccgaaactaa ccataaagtt
     1201 aaccaacccg atgattccga tgccaaatat tccccaaaat aacatccaat tatccaaggt
     1261 ataatttgaa ccataaagtc caagagccag atttccactg gcaaaagtaa tgtgtctgat
     1321 atctaaattt aacccgaaaa agacacctat tgaggcagtg gaacccataa aaataccaaa
     1381 ccaaaaattt gaaataatac ctgcccattt tttttcatac cagtgagcaa atctttcagc
     1441 tttttctttc ccaaaactgc gttttaaaaa gggatgttct ttaattcgga gataaatttg
     1501 ataatgttta tcgcgattag ccacacttcc ggaaatgatt cccgacaaaa acaaaaagca
     1561 acctgcaata gccgcatgaa aaatagccat cgaatgaacc ggacttaaat ccgtcactaa
     1621 agtattccat ttcgtttcgg caatattata gtgaaaagcc aaatcaataa gccaaattcc
     1681 caataaagat atcgggaaag ccatgatgac attcccaaca aaggcaatga attgtgaacg
     1741 gaaaacgcga gcaaaaaata cagcaaatcc ttggtgtttt tggttcgaat ttccgttttc
     1801 ttttatttcg tcttccagca ctttaactaa agcggaagcg gtcattgcgg gctgttttgt
     1861 agccaatgta aatcctaaaa cataaatagc aataaaccca aaagaataat tcatactata
     1921 caaaaaagca tggccaaaat cactggtttc cacttttgag agcaaaactt tgatgacaca
     1981 caaaatcccg actattaatc caccacccaa agctgtattg aacattttaa aatattcgga
     2041 tctggattga gtgatatatt tttctccggt tttagctgta tgttgtgtga tttcatagga
     2101 aagcaattgg gtactttctt ttataaatgt tcgaacatta tttcgggtac aattgtattt
     2161 aattaatttg taggccaatc ggatactgtt ttttttctta tccgtttcaa catctactac
     2221 taaaagcgga ataagaattt ttaatcgata taactgttgc ctgattcgga gtaaactttg
     2281 attcacccga agtgttatac caaatttaga agaattttga aatgcttttt ctacatattc
     2341 ttcacattgt ttatgcaaaa caaaaagctg tttgtaggat aaactttcgg atgttgtaaa
     2401 atgattgttg tcttttgttt taattttgtc ttgaattgat aaaagttctt tttcgaatgc
     2461 cgcaaaaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tgaaaattga
     2581 tttgtaattg atttgttttt tttcaaaaac agtagtaatt ccgtaaggtc tatttcatct
     2641 gtttttttga gatttttagg ccgaattgaa gctacaattt taactagaat ttctaacggg
     2701 tcaattgttg tttcccaccg tttattatta tcaaaataaa cagttaaaac atcaatcgtt
     2761 gttacttttt tctttttagt aaaggataca gccatttggt atatttgttt tgtaaaaata
     2821 cgaatcaaaa atatttatat ctaccttgca ttcatattat atgaaaatat tatgatagaa
     2881 attgaacgaa aatttttagt tacttctcca caatttaaaa aagaagcttt tcaggaatcg
     2941 agaattgtac aaggctattt aaattcacat cctgaacgta ctgttcgcat tcgaatcaaa
     3001 ggaaataaag ctttcataac catcaaaggc attggaaatg aaacgggttt aacgcgtttg
     3061 gaatgggaaa aagaaatttc tgtagccgat gcggaaaaac ttttaaaact ttgtgaaaca
     3121 ggaattatcg acaaagttcg ttacgatatc aaaataggta atcatatagt tgaagtcgat
     3181 gttttctccg gcgaaaatga aggattaatt atggctgaaa tagaattaga atcagaagaa
     3241 gaagctttcg aaaaacctta ttggttggga gaagaagtaa ccaacgacga acgttattac
     3301 aatgcttatt taagcagaaa tccttttaaa aactggagct gactcaattt attgtgtctt
     3361 ttatttcttc aactaattca atcgtcactt tcatcgagcc atatccatta tgtctggcaa
     3421 tatcctgaaa agctctttta gacaaatcaa tttctctacc tttcgtaaag ggtccgcggt
     3481 cattgattgt tacaatagtt gattttccat tagcttcatt ggttacccta acttttgttc
     3541 caaatgggaa tttccggtgt gcagccgtgt gcttttgatt atcaaaaact tgtccgcttg
     3601 cagttctttt ccctgtaaat ttatccgcat aataagaggc atgagctcct tttttataca
     3661 atttaaaggt aaaattgcct tctgttgcaa tgctatcgat ggcagtttta tcatttttag
     3721 tagtatcgct atggataatt ctatttaatg atggtttttc gaaatgcttt gtttcaaaag
     3781 aaaaactact gaaaaacaat aaggcaaaca gcccaaacac aatcagctcg gttttatgtt
     3841 tcataatcca gattttaagt gcgtttatca ttaacaaata gtgttccaaa atgagaaaac
     3901 cacgaaaaaa cgtggtttta aacttttatc ctaaccaaga actccaagga attcgaatta
     3961 aaataagtaa taatcccaac ccataaaaaa ccataatttt ttgaaattta aggttgttgg
     4021 ctactttttt atgttttgac catccaattg taattaatat aatcgcaatg atgttgatta
     4081 atggatgttc catcgatgtt aatcgaatag ccgggtcttt catttgtcct aacgaatcta
     4141 atcctaaagg agaaactata tataaaataa gccctaataa caattgaata tgagtaaaaa
     4201 tcaacgcaaa aagtgaaatg cgtaaatctt taggttgaaa cagttttttt ccggtataac
     4261 ctaaaacagc attaataacg gctaaaaaaa gaagtattag agccaaataa gcccaccaag
     4321 aatgtaattt ttgtaataat tcgtacataa gaaatttatt ttatgaaaaa caaatatagt
     4381 aaaataaaaa accctatcaa gcagataggg tttcgatttt tatctttggt ttaaaaaatg
     4441 ggtcaataaa aagttggttg aactgtcgtg gtttttaatt ttaccggaag taatttcccc
     4501 taaaatagaa ttggctaatt gtttgccgag ttctacaccc cattggtcat aactgaaaat
     4561 attccaaata acaccttgta caaatatttt gtgttcatat aaggcaacta aagaacctaa
     4621 agtctccgga gttaaactgg taattaaaaa agtatttgta ggtctatttc ctgaaaatat
     4681 tttaaacggc aataaaaatt ctgccttttc tccagaaact ccttgttttt caaattcagc
     4741 cataacttgt tctgcaggtt tcccgtttaa taaagcctcg gtttgtgcaa aaaagttgga
     4801 cattaactta tcatgatggt cttgatttcc atagagtgaa tgtacaaatc caataaaatc
     4861 agtaggaatc aattttgtgc cttggtgaat taattgaaag aaagcatgtt gagaatttgt
     4921 tcccggttct ccccaaatta ttgttccggt ttgataattc accgggtttc cttcacggtc
     4981 aatgctcttt ccgttactct ccattattcc ttgttgaaga taaggagcta atttttgaag
     5041 atattgggta tacggaatca aagcttcact ttctgcacca aagaaattat tataccagat
     5101 acttaataaa gctaaaataa ccggaatgtt tttttcaaac ggtgttgttc taaaatgctc
     5161 atccatgtca tgagctccaa tcaataactt gtcgaaattt tcatagccaa ccgctaagct
     5221 aatggataat ccaacagcac tccaaagtga aaatcgaccg cctacccaat cccacattgg
     5281 gaaaatatta tcaggatgaa ttccaaattc tgtcactttt tgaatgttag tagaaacggc
     5341 gacaaaatgc ttggcgacat cttcttgctt agctgatttt aaaaaccaat ggcgaattgt
     5401 ttcggaatta gataaggttt cttgagtagt gaacgtttta gaaacaatga caaataaagt
     5461 tgtttctggg tttacttttt taataacttc gttaacatga tcaccatcca catttgaaac
     5521 aaaatggaca ttcagttgat ttttataaaa ctggagggct tcaaccacca ttgcggggcc
     5581 caaatcagaa cctcctatac caatgtttac aatatcagta aaaggctttc cggtaaaacc
     5641 ttttcgttca ccattaacca cttcttccgt gaagttttta attttatttt ttacggcaac
     5701 tacttccggc atcacattaa ctccatcaac cataatactt gcattagtag aagctcttaa
     5761 tgcagtatgt aatacggctc tgttctctgt ttggttaatt aaatcaccgg caaaatattt
     5821 cgatatagca tcttttaaat caacttcatt cgctaaagcc aaaagatgtt gttttgtttc
     5881 ttcagtaaga atattttttg aataatcgac taaaaagtcg ttccattgaa tatggaattt
     5941 ttctgctctt ttagcatcct tagcaaaaag ttcttgcata gaaacatgtt gcatttcttc
     6001 aaaatgtttt ttcaaatttt gccaagctgc tgttcctgaa ggattggtac tttttaaggc
     6061 catgatatca attttatttt ttaaactgta gtgttgcaat gctgtctaac tcccttttta
     6121 aaggagcgat ataagttaaa taatttttcc ggtgtttttc attcatcggt tcagtattgg
     6181 gtaatttttg tgataaagga tcaacttgaa caccattttt ccaaaagcga taacacacgt
     6241 gtggacctgt ggctaaaccg gtacttccca cttttccaat cacctgacct tgttcgacac
     6301 gttgcccttg tcgtactaaa attttggaca tgtgtaaata ttgagtcgaa taagttccat
     6361 tgtgtttgac ttttacaaag ttcccatttc cggctgtata tcctgttttt tcaaccactc
     6421 cggaagctgt tgttttaata ggagttcctt gtgctgctgc ataatctgtt cctttgtgag
     6481 ctttccaagt catttggacc ggatggaaac gtttaggtga aaaacgagag gatattctaa
     6541 aataatccaa cggagccttc aaaaacatag ttttcaatcc ttttccttct tcatcataat
     6601 aatccacttt tttggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa
     6721 catagttttc aatccttttc cttcttcatc ataataatcc acttttttgg ataaactgtc
     6781 taatttgtat ggaaaagcgt agattttttt gcctttatgt tcaaaaaaag aactttccat
     6841 tctttctaca ccggcataaa tgctgtcgtt gatatatttt tcataaaaag ttaccgcaaa
     6901 tttatcatct ttttgaatct taaagaagtc aattgaatac tcataaattt ttgctaaatt
     6961 atttgccatt gaagcatcga caccattttt acttaatgtt tccgacaatg atccactaat
     7021 tgacgtagca atagttcttc gttttatagt gatgggtttt tgtttattat aagcgaccac
     7081 cacagtatct ctgaaatcaa caacactata atgaatatta tctttctgat aaataaaaac
     7141 ctgtaattgg tgaggggctt tttttgattt taaaagcgta aaaggtttgc caattctaat
     7201 atcccgaaga ttaaaactgt cttttaccat ttctgtgaca tcatgcactt taaaagtgcc
     7261 gatattgttt tcttccaata atttactgaa tgtatctccg ctttgaactg tatcttgaac
     7321 tactaaataa tcattgtacg taaacccaaa ctcaacaatg attggctctt ctttaataac
     7381 ttctttagga acaacttttt tatctttttc ttcgcaggcg aataagagta aagtgattag
     7441 agatatcgtt actattttaa atttcaatgt tttttcttta aatcaattat atactttctt
     7501 tttctcccca agtggccaat tcctcagctg tccatagttc agggaagaaa attcttcgtt
     7561 ggtatttggg caacatgtat tttttccaat cactgcctcc tgttgcttct ccggttccgt
     7621 gtccgctttc aatatatttt tttgctgcat gataatgacc cattacccaa gttatgttga
     7681 cggtataatc ataatgtcgc atcgcattaa ctaaagcttc gtttttttgg tcgttttcag
     7741 gtaattgttt gaatttccgc cacaaattag tggtgttgta ttcttccata aaacgcaaaa
     7801 attccttttt atattttcgt tcgaactcta aaattaaaaa agatttttcg ccggtttggt
     7861 aatctttccc ggctgcttgc caatacaaat gttcaaaagc atattcaaaa ggcgtatttc
     7921 tatctatcgt tgctctaaaa cggtaatcaa tcaagttaat caaatcagtt gaggaaaatt
     7981 caataaatcg gtattgagca ctttgaaaac cactggcggg tgttaacgta tttctgaatt
     8041 tcatgtattg ttcaacatcc attccttctt tcataatggt aaaagaggtt gttaacatat
     8101 cgaaataacg actgattctc attaattttt cggtgaaata agctgtttca ggttgctcgg
     8161 tttggcagag ttgttcaatt tcccaaagaa tcattttaaa caataattca ttcacttgat
     8221 ggtacataat aaataccatt tcatccggta aagttgttcg ttgggtttga agattaagca
     8281 aggcatccgt ttggatataa tcccaatagg ttattggttt tgcccacaaa agtccttcta
     8341 aatgtgtatc agttttttga tttatcgcct gaaatttttg ctctatctgt tctaaaattt
     8401 ctttgtgtgc tgttgaattc attacggttt tgctttaacg gtaaatgtgt gttttaggcc
     8461 cttgaattgt tccaaatctg cttttattga acctacagct aaactggctt taattcgaac
     8521 gggtaattta ttgtcatcat ctgatatcca aacagttaag ctctcttgct ctttaaaaac
     8581 acgaccggcc tgaacatagg gtctgaatat cattgtggaa attaccccaa atttagtttt
     8641 taaatcttca cgaccaataa atttcaactt aaatttagtc gtttcatcat caaaaaacat
     8701 atcaacatta attgactcac ccactttcag tttgtcaaca ttagggtgat ttcgtaaata
     8761 gtaaaaggta gataaaatgt cttgggtatt atcaggaact tcaaaggttt tttcagtttt
     8821 tttcttgtaa tcctttacta aaatggtttt tgatttatga ttaaaaaacc cttcctgatt
     8881 ttttgtataa ccgccttcat ctatttttcg tacaaattga tagggattcc ccgattcttt
     8941 gtcaaaataa ctttggtaat catcttccac ttcaaagaag aattttgtca ttccaacggt
     9001 ataaccccgt cccttggcat gaaaaacctt tttattgttt ctaactgctt cttttacttc
     9061 taattccgca taaccggcat tgattaagcc gtaatggata cgaaacttaa accattcccc
     9121 aacttcaaaa gcatcttctt ttttcggttg taatgttgga gaagaatcga aactgacagt
     9181 cgaaaagaaa ataacaaata aaaataactt tttcatagta gtgcttgatt ttcttcagat
     9241 aaatcaattt ctgttccaaa tgtactaaaa ctaaaaaaga cctcaaaaaa aggaaggact
     9301 ttaatatttt tttaagagtt ggaaaaaagg gggagaagtg ctcaaccaat gctgaagttt
     9361 cgcgatataa aacacaaaac ccaaccgtta ccgattgggt tgtatgctat taactaacca
     9421 aaaaactata aattatgaaa aatttatatt acactaagaa ggtaaccact ccctcttttg
     9481 cgagtgcaaa ggtaatctgt tagttatatt atttttaatg aaaattcaac tttaactaaa
     9541 tatttgtaaa aaaatagcag tttataggtc aaaaatttca ttatctgtaa ttttcaggtg
     9601 gttttataac gttcctctta aagcttgctc tctttctatg ctctcaaata acgctttaaa
     9661 gttacctgct ccaaatcctt tagctcccat tcgttgaata atttcataaa acaacgtggg
     9721 tcggtcttca acaggcttgg taaaaatctg taataaataa ccgtcttcgt ccgcatctac
     9781 taatatggaa agtctttgta attctttaat atcttctttc atcatatcca tatgaacacc
     9841 taagcgacga ggaatatcat catagtaagc ttgtggtgga ggcggaagaa attctactcc
     9901 acgggatttt aatccggcaa cggttttaat aatgtcatct gtggcaacag ctaaatgttg
     9961 aactccggaa ccttcataaa aatcaatata ttcttcaatt tgagatcttt tgttcccttt
    10021 tgcaggctca ttaattggga attttattct tccattacca ttactcatta ctttactcat
    10081 caaggcagaa tattcggtgt gaatttgtgt atcatcgaaa gaaaggaaat tttcaaatcc
    10141 catcacatct tcataccatt ttacccattg gttcatttca ccccaaccga cattccctac
    10201 catatggtct acatatttta atccaagtgg ctccggattg tactctgatt tccattctac
    10261 aaaaccgggt aaaaaggctc cgttgtaatt ttttctttct acaaagatgt gaaccgtttc
    10321 tccataggta tggattccgg aacggacaac ttcgccatgt tcatcttttt caacggttgg
    10381 ttccataaat gatttagccc ctcgcttagt ggtttcttcc catgctgaac gggcatcttc
    10441 cacccataga gcaatcactt tcactccatc tccatgttta gcaagatgtt cattgatagg
    10501 tgaactactc gttaatggcg ttgttaatac caatcgaatt ttatcctgtt ttagtacata
    10561 actaacggaa tcttttgatc ctgtttctaa tccgcgatag gcatgtgatt gataaccaaa
    10621 tgcggttttg taataatgtg ccgattgttt tgcgtttcct acataaaact caacataatc
    10681 tgttcctaaa agtggaagga agtcttgtgc tccttcaaat attttttcca gtccgtattc
    10741 aacggatttt acgtcttttg ccataatttg ataattagtt gatgagcgaa tgagtgaatt
    10801 ctagttattc acttattcaa taattgtttc acttaaacct tgttttttga agtgcttatt
    10861 attttgttta acatttttat aatcccgttt aatttttcaa ttagctcttt cggattaggg
    10921 taattttcta aattttcaca taaaaaaagc caatattctg tttcatctgc ctcttttaaa
    10981 gagattttga gcttatgaat aaaatctcct ttgctttctg cattttgagc ttctttaata
    11041 tttgctccaa ccgatgtccc cgaacgaatt aattgttttg caataacata ttttctgttt
    11101 tcatctaata attcggaata ttttacaacc ataagagcaa attcaaatga ctcttttaaa
    11161 attgggttat tttgatattt tgaactattt gtgaaaatca tatgtattga ttgatcagcg
    11221 tatgatagga attcgctcat tcgcttatta cataattcgc ttattatagc cagctttgaa
    11281 aatagttttc atcggctatt ttcatcgctt cctctgttac cattaaaggc ttgaacgtat
    11341 cgaccattac cgctaactct agcgtttctt tctttccaat acttctttcc gcagctcccg
    11401 gatgaggccc atgagcaatt ccggcaggat gtaaggaaat gtttccttgt tttatgtcgt
    11461 ttctactcat aaaatcacca tcaacataat acaaaacttc atctgcatca atattactgt
    11521 ggttataagg tgccggtata gctaatggat gataatcata tagtctgggc acaaaggaac
    11581 agacaacaaa tgccgttgtt tcaaaagttt gatgtatggg tggtggaaga tgaatacgac
    11641 cggttatagg ctcaaaatca tgaatcgaaa aagcatatgg ataattgtat ccgtcatacc
    11701 caacaacatc aaaagggtga gaagcatata ccatttcaaa aatttcgtct ttctttttta
    11761 atttgatgat aaaatcacct ttttcattat ttgtttctaa ttcatacggg cgacgaatat
    11821 cgcgttcgca atacggagaa tgctctaaca attgtccaaa ccaattgcga tattgcttag
    11881 gtgtataaat tggagcgtgt gattctacaa taaacaagcg gttatcttcg gtatcaaaat
    11941 caattttgta tataataccg cggggaacga taaggtaatc tccatattta aaatcaagat
    12001 ttccataaat tgttctcaat ttaccggaac ctttgtgaac gaaaatcatt tcgtcggaat
    12061 ctgtattttt ataaaaataa tccgtagttg attttctggg agcagctaac acgatgtgac
    12121 agtctgaatt ggtaagcaca atttttctgc tttccaaata atcgtcttga ggtgccacat
    12181 caaaaccact caatttgtag gactgaatat tgttagattt agcaattatg ggagcgatac
    12241 tgtattgtga acgaatttct tttacttggg taggtcgttg ttcgtgataa gaattagtcg
    12301 acatgccgtc gaaacccacc gtgccgaaaa gttgttcata atacaaatcc ccatttggtt
    12361 tacgaaattg tgtatgacgt ttgggtggaa tatttccaag tttatgataa aatggcatag
    12421 ttttaaaatt agataattaa taaaaaacgg ataattgaat tagttcgtga actgattcaa
    12481 caaaagtatc actccggatt tctcttttga agaaaatgga ggtagtctaa catccctcgc
    12541 caaaatgatt tattcatgaa gaatgatatt atttcagttg tttttcttga tattaattcc
    12601 cacaataatt cctaatgcaa aagccaataa caaccctaga attataacca ttatgctgtt
    12661 ttttttatat aatatacaaa tatcgtaaaa tttattaaat aatatgtaaa attaaaacca
    12721 aaaacctacg ccaaaccaag tgaatgaatc tttggtttcg ggcgaccaac tttgtttaat
    12781 ttcgacaggg cctaacaggg tttccaaacc atagccgacg gaataacctg aaaacgtggg
    12841 gagttttaac caaccgtcat tcacaaaaat atcataccca atattagcat aattggccgc
    12901 tacattaata tggttttttt tgaaaatttc ataatcaaaa cttaaactgc ccattacata
    12961 actatttccg gtcaaactaa caaaatcata cccataaaaa tgcctgaaat tgttaaatgg
    13021 tttataacca taccctccca taaaaaagtt taagatagga atgctttgct caccaaccgt
    13081 aaaacctccc tctgatttta taacaaacga gccttttctg aataattttt gagcataagc
    13141 cgcttctcct ttgaaaatag tgtgcattaa aaaaatatct tcatgtctgg aagaatataa
    13201 aaaagactga acatccccat taaaatacca tccttttttg ggaaaatatt tattatcaaa
    13261 ggaatcaaaa ttcatatttc caaaagcaga taaataatcg ccattatcaa aaatagccgt
    13321 tgaattagaa agtgtttctg attggatttt aagattcata agctctaccc caattttcgc
    13381 actaaatttt tggacaaata gtgtttggag atataattgg ttcgtaaaat ctctataatc
    13441 aatatttatg gaattagtac cggcttctct taaaacatcc catgcactaa aatttgtgtt
    13501 tacatttcga ttaaattgat aaaatttaga atttaaacca acactcaaat tataaccaat
    13561 atcaacatta taatctaaga aatacctaaa attatctccc agaactacat ctgcggaaaa
    13621 aacatcattt ttaaataata attttttctt ggtatagttc actaaaatgc cgcttttaaa
    13681 caatccgtca taatgaagcc ctaactttaa atacgttttc atcggatttt ctactaaaaa
    13741 aagatttaat tggtcgcctt cttcttcttt ttctaacgta taacttaacg aactgaagtt
    13801 ttgagtagta tttaaataat caatacccct aactaagtcg gcatagctta ttttttgccc
    13861 tggtttaaat cttaatttac tttgaatata atattttgtg taattttcaa gtgggttgat
    13921 tttgatagaa ctgatgtata tactatccgt ttcatttttt gggaacaccg tagttggtct
    13981 tttataattg gtgctcaatt ttttaaaagc ttccatatat tccatggcaa catcttctcc
    14041 aatttttata atttcaggcc ctttatcaaa agagacaacc gtaaattcat tgatgctggg
    14101 cttgatgtaa atatcgatga ggtgcttttt ttcctccatc ttcttaatca tctgtaaatt
    14161 agatatttga accattaatt tagtagcctc ggtaagttcc tctctttctt tcaattcata
    14221 ttgaacatca acaccaatga taatgtcggc tcccatattc ttgacttctt ctaccggaaa
    14281 attattcaaa accccaccat cgattaaaaa cctaccatca ataattaccg gagcatacaa
    14341 tgtcggtaac gcaccactag ccacaattgc ttgagataaa aaaccatgat ctaacaaaac
    14401 ttcttgtcct gtttctatat cggttgccat acaaacaaaa ggaatgggca attttttaaa
    14461 attacgctca tgacgaacat gatccatcaa ccgtgtcatt aaattataat tatacaaacc
    14521 tctggaaagg gcaatcggca cccctaattt gaatttatta aagggcaatg tcaacgcata
    14581 acgatcatta ttttcgcgtt cgtagtaatc tttagaaact ctcggagtgt aatcttgtat
    14641 aatcgcatca taatcggctg ctttaaaaat actgtctatt tgtataccgg tatagcctga
    14701 agcatacaaa ccgcccacga ctgctcccat actggtcccg ccgatgtaat ctatttttat
    14761 tccggcttcg tctatttttt ttaaaacccc aatatgagcc aatccttttg caccgcctcc
    14821 actaagcaca agaccgattt tgggtcgtgg aagagtgtct tgcgaaaaac aggtaagagg
    14881 aaataggaat agaattagca ataataaaca taacccaatt ggtctgattc gagtaggtat
    14941 gctcaaaaaa gagaaaggga aaagcgttaa aatagcaacc aaaaaggccc gaacgtctcg
    15001 cttcaaaaca agttatttat ttattgcttt gtaaaattcg taaatttttt tggcttttgc
    15061 tactccaacc accgttgaaa tttcattttc acttgcctgt gctattcttt taacactttt
    15121 gaaatgttta attaacgtaa ccatggtttt ttcaccaatt cccggaatcg tttctataga
    15181 agtttccaaa gcagatttac tccgtttatc acgatgatga gtaataccaa atcggtgtgc
    15241 ttcatttcgc agttgttgta taattttcaa ggtttccgat tttttatcca aatacagcgg
    15301 aacagtatca ccgggataaa acaactcttc taatcgtttg gctattccga taatagcaat
    15361 ttttccacgt aagcctaaat catctaaact tttaagggca gaagacaatt gtccttttcc
    15421 gccatcaatg atgatcaatt gtggcaacag ttggttttcg tctaacatgc gtttgtaacg
    15481 gcgataaact acttcttcca ttgatgcaaa atcatccggc cccactaccg ttttgatgtt
    15541 gaaatggcga tagtcctttt tacttgcctt tccatcttta aaaaccacac aagccgcgac
    15601 gggatttgtt ccttgaatat ttgaattatc aaaacattca atatgtcgag gttctactga
    15661 aagtcgcaaa tctttttgca tttgagccat gatacggttg gtatgccgtt caggatctac
    15721 aatttgaatt tgcttaagtt gctccaaacg atggtatttg gcattgcgtt gagaaagctc
    15781 aagtatctgc tttttatcgc ctacttgcgg aacagtgacg tttatcttat caccaaaatc
    15841 caaggcaaaa ggcaaaataa gttccttgga agttaaatga aatcgctcgc gtaattcaac
    15901 aacagccaat tctaataact cctcatccgt ttcgtcgagt ttctttttca attccaaggt
    15961 atacgaacgg ataatcgctc catgagcaat ttgaagaaaa ttaacatagg ccatcgtttc
    16021 atctgacaca atggaaaaaa catcaatgtt ggaaatcttc ggattcaaaa tggttgattt
    16081 ggcttgatag ttttctaaaa ctcctatctt ttccttcatt ttttgggctt cttcaaattg
    16141 catggcggcg gccaactcat gcattttaag cttaaactct tttaaacttt ctttaaaatt
    16201 acctttcaga atttgccgaa tggcatccac ttgtttctga taattttcta acagttcaaa
    16261 accttcacaa ggtcctttac aattaccgat atggtattct aaacaaacct tgtatttatt
    16321 gcttttaata ttggcttcgc tcaaatcata gtgacaagtt cgcaacggat acaactcttt
    16381 tattaaatcc aacaacgtat gaaccgtttt aaaactggta taaggaccaa aatactctga
    16441 accgtcttta atcatttttc gggttgaaaa aatacgggaa aaaggttctt ttttaatgca
    16501 aatccacggg taggttttgt catcccgcaa cagtacatta taccggggct gtaatttttt
    16561 aattaaatta ttttccagca aaagagcatc ttgttcagta gaaacaacaa tgtgtttaat
    16621 ttcaacaatt ttacgaacta atacattggt tttggcattg tcatgcgttt tattgaaata
    16681 ggaagtaacg cgttttttga ggtttttagc tttccctaca taaagaattt tcccctcttt
    16741 atcataatac tgatacaccc ccggattttc cggcaaggtt tgaagttgga gtgctaaaga
    16801 aggattattc atgtaacaaa gttaattgga tatttaagat tttcaaatga gcggccacga
    16861 aaaataattt tgttattttt atccaatgaa taaatctgat ttacggacgc gatataaatc
    16921 gcttcgtgca caacttactg cggaagaaat agaagaaaaa agcttggcca tagccaacca
    16981 tcttttacag gttgacattt ggaacaaaac atattaccat ttatttctgt caattgtgga
    17041 acaaaaagag attgatacgg aatttatttt acaaatttta gccggtaaag acaaagaaat
    17101 tgtgctggct aaaagtgatt ttaaaaccgg agaaatgacc catttcctat tgacggacaa
    17161 tactaaaatt aaaaaaaata actacaatat tccggaacct gttaatgggt tggaagtccc
    17221 tgtgtcaaaa atagatgtcg ttttcgtacc gcttttagcc ttcgacaaaa aagggcatcg
    17281 ggtgggctat ggcaaagggt tttatgaccg ctttttaacg gcttgcaatc cggaaacgct
    17341 taaaatcggg ttgtcttttt ttgaagccga agaaagtatt gaaggcactt cagccaatga
    17401 catccctttg gattatgtgg ttacgcccga aaaagtgtat ttttttgatt aagcagcaac
    17461 ttccgttggt tgttctttat cggtggtacc gaacgctttt ttattaaaca caatcaatat
    17521 ccccacgcct gtggaaatag ccatatcggc aatattgaaa atagcattaa aaaaagtaaa
    17581 atacttaccg ccaataatag gaagccactc cggtaaagtg cccttccaaa tcggaaaata
    17641 aagcatgtcc actactttcc cgtgaaacca agttccataa ggttgttctg aaaacaatac
    17701 agcgggtgca ttataactat cgttgaaaat taccccataa aaaacactgt ctataatatt
    17761 tccaaaggct ccggctaaaa ttaaagagac ggccacaatt aaataggtag aacttgcttt
    17821 tcgaacacaa tcccataacc aatagccaat gccgaatacg gctacaatac ggaaaagggt
    17881 taaaattaat ttaccgtaat cgcccggaat ttctgtcccc caagccattc cttcattttc
    17941 aataaagtga atccgaaacc aatcaaatac tttaatttct cctcctaaaa taaaatgggt
    18001 ttttatatat aatttagaga cctgatcggc taaaagaatc agaaaaacaa tcagataggc
    18061 cttttttaaa gtcattttta aatttttatc gtgcaaaaat aggaatatta tccatcaaaa
    18121 atgcattatc aaaggctcac ctaaaaattt taggtttttt ttaatctttc acgcaacttt
    18181 tctgcctcaa ttgtatctaa aatttataac aatagtattg aaaaagtatg taattttgtt
    18241 aaacctaaac caatttataa tgaaaatagt aaaatttgtt gcaattgctt ttgtacttag
    18301 cttttttgct tcctgcaaca acgaagatga taatggtgaa atggcaaaat ttgctgttcc
    18361 tgaaatcaaa tcattggcct ctatccgcag tagcatttcg gtaacccctg cccaagcaac
    18421 caatgcagac ggaaaagtgt acgtagcgga aaatctctta ttttatgtgg ctcaggaatc
    18481 tggtattcat atttttgaca actcaaatcc cgcaagtcca caaaatacag cgttcataaa
    18541 cttagaaggt gttcacgata ttgctgtcaa aggaaatttt ctttatgcag ataattttat
    18601 agacttagtc gtatttgacc tttctaacct gggaaacatc acggtggttc aaaccgtaca
    18661 gaatgccttt gaattttatc cttcctatcc ggaaggagct gaattttatg cctttgatgt
    18721 ttatcctact gctaatgaaa ttattgtcgg ctatcgttta gaaaacagaa gaagacccga
    18781 aggtcaagat ttaatcttag ccaacgatgc gttggccgga tttgaaagct ctgctgcggg
    18841 tgctgtcgga acaggtggct cctatgcgaa gtttcaaatc aataacaatg ctctttacac
    18901 agtggaaagt tatgaactga atgtatttaa catcagtgac ccaactcaaa ctttttttga
    18961 caaagcggtg tatatgaatg aatggctggg cggtggtcaa tttgaaaccc tcttcaaaca
    19021 aaaagaattc ttgttcatcg gttccaccaa cggaatgtat attgtgaatg ctcaagatga
    19081 atttaatccc tatttcgttt ccggtttctc ccatgctacg gcgtgtgatc cggtagtggt
    19141 gtttgccaat acggcttata ttacagtgag aggcggttct acatgcggtg cgatagaaga
    19201 ccaagtgaat gtgattgaca tcaccaacat cgaaaaccca accttaattt caacgtattt
    19261 gttaagtgat ccgcgtggat tggggataaa agataatgtc ttatacgtgt gtgccgacgg
    19321 aaacttaaac gtattcaatg caacaaactc ggctgaatta acccttcaaa acacctactc
    19381 ggatggtgtg caagatgtta ttgctttaaa ctctcacctc atcgcggtgg gcaacaataa
    19441 aatcatccaa tacaattatg gagaaaatca ttccttagaa caaattagcg tggtgaattt
    19501 ttagtcttac cccacaatta aaaaaaataa gcctactcaa attataattt gagtaggctt
    19561 attttttaat catcatagtg aaatctacac tgtaatatga tacacctttg atcaacaccc
    19621 ttcgtgccta aaactttgta aacaattcta tgttctccgg ttatccttct tgaccaatat
    19681 ccttttaaat catattttaa gggctctggt tttcctaaac ctttgaacgg atcttgtcgt
    19741 attgatttaa ttaaatcttt aattctagta ataatttccg ggtcattctc tatccaatat
    19801 tcaatatctt cccaactatt tggcgtaaaa ttaatgtcca taattaaaca gaaggtgtta
    19861 caaattcatc tttaaggaag tcattaccgg attgaatcgt tctcaattct ccattttcta
    19921 attgttgaag ggcatcagca agtctttttc tattcgtttt agtagataat aaatgttgtg
    19981 tttctgtcat ggaattgtac tctttcaatg atataatgac aacagcaccc tcctcatcat
    20041 ttctagacac taccaacatt tctgatgatt cagttacttc atcaaaataa tttttgatat
    20101 tttttcttaa ggtcgaaatg gatactgctt tcataatagt gtttttaatt gtacctctaa
    20161 ttgtacgttc aaatgtacta aaaagttacg actttccaaa aaaaagaata ctttttgtaa
    20221 actggaatat aaatttagtg catagttcag aagaaataat aaaagaaagt agtcataaca
    20281 tctgcttgaa tcgaaatgaa aataaccaaa gaaacaagtt gttcaaaagt agagaagttg
    20341 gaaaaaaaat gagatacaca aacctgaacg tgctgcctta ctcaactaaa acaaaaaaag
    20401 ccccgcatta gcgaggcctc ttcaactatt taaagacaca ttaacgctgc atgttcttcg
    20461 cctc
//
LOCUS       DAOTCW010000029        20382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1415_length_20382_cov_38.707581, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000029 DAOTCW010000000
VERSION     DAOTCW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 20382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20382
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1415_length_20382_cov_38.707581"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1174
                     /locus_tag="P5335_03985"
     CDS             74..1174
                     /locus_tag="P5335_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ74072.1"
                     /translation="MKKITLLFFALILAEANAQVDDVSPNLSNNSFPNYSSLFNPYTT
                     EFLYDIGSAIGTQGNAGVLFLNNQFWVSAWASSNIHVLNNSGGFVETFTVAGVTGARS
                     LTTDGTNVYVGANNTNIYVINPITKTLINTITTTTTSGINIRFLTYDATLNGNAGGFW
                     IGNFNTDIVAVDMSGNQINVIPATTHGLTGMYGAAVNSATNTLFVYNQSAPSNDIISS
                     ISLSTGAMLVNYDVFANDLSSNGVTSSLAGGTFLSSDIVPGQTILIGVSQATPSNILW
                     GININNLLSTTDLEKDSFKIYPNPVNNLLHFSTTNFDEITIFNIDGKIIKSLKSNSLN
                     DKTLDVSNLSKGVYIISVKSESNIKYFKIVKV"
     assembly_gap    1306..1405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1406..2601)
                     /locus_tag="P5335_03990"
     CDS             complement(<1406..2601)
                     /locus_tag="P5335_03990"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRZ74073.1"
                     /translation="MIPLFNINRWVEQNFGHLTWEQVQSWFVFDPQKPLLFNSALFLG
                     LFLVFYFFYILTQKTHRLRIAYVVAFSLFFYYKSSGIFFLLIIFSSVLDYVIAKLIYD
                     EKSIFYRKFYLIVSLLVNLGLLGYFKYTNFFIDNTNLIFDRNFEFQDIILPVGISFYT
                     FQTLSYTIDVYRKQLEPTKSFVDFLFFVSFFPQLVAGPIVRASDFIPQIYKRLNLTKE
                     EFNAALFLIVGGLLKKAVISDYISINFVDRVFDAPNSYTSFENLMASYGYAIQIYCDF
                     SGYSDMAIGLALLMGFWLPPNFRTPYQSASVTEFWRRWHISLSTWLRDYLYISLGGNR
                     NGKFRTYINLFLTMLLGGLWHGASWKFVFWGTLHGLALAFERFFGNFIKLPKNHFIKT
                     LQILLT"
     gene            complement(2598..4004)
                     /locus_tag="P5335_03995"
     CDS             complement(2598..4004)
                     /locus_tag="P5335_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRZ74074.1"
                     /translation="MHNKFFSFLFFLLFSFSLCAQSDSLFVEIDTTEVITAINEIKNN
                     KVLNPIFEKLVNLQKSKVGKLNVVHIGDSHIQADLFSGLLRKNLQQQFGNAGRGLVFP
                     HSLVKTNGTADVRFSSNQTWESQRNIYPNTGNPVGLSGIALFTKSNDFALEMTVKNQE
                     YVFNKLKIITPQNIKMFEVALTSKTIKIESEIPKNILHKIKNGESLSVISEKYDVSIA
                     QIKKENGLKNNNIRAGKTLKIPSKEKAKKVVERSEFIPLETRLEEGFHSFETDTLLHK
                     IYLIPALNQSDFALNGLVLENNNPGIIYHTIGVNGAKCSDFTKFPLFFEQLAVLQPDL
                     VIISLGTNESFDKMITSEFIVQLNLMMELIFAKNPTTVFLITTPPPSQFKRKFPNTFV
                     ADYTKEILAQADLKNYAVWDMFSNFGGLFGVNQLVKEGIIGADKVHYTKVGYERQGQL
                     LTEAFLSSFENYKLEINK"
     gene            complement(3994..>5282)
                     /locus_tag="P5335_04000"
     CDS             complement(3994..>5282)
                     /locus_tag="P5335_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRZ74075.1"
                     /translation="KTDEKTIDTFYRKKIVFEQIEGIAYPPEEYGQYKGFQYLIPFFE
                     KLYQLETKQEGDIRIAYFGDSMTDGDLIVQDFRKAMQTQFGGEGVGFVSITSESAGSR
                     SSISHQYSGNWKTQSYLNVKRPLKPFGVNGHVFFANDTLSDTWVRYKAGFGMHTSQLN
                     NPTLFYGNSSNKMGEMLIKIGKDTLIKKLNPEKQLNALAISTTNLKSFKAVFKQADSI
                     PIYGFNFDDGKGVHVDNFSNRGNSGLPISTFNTNLMKQFQERLDYDLIILHYGTNVLN
                     YGTYNYNWYEKGMTRVVNHIKECFPGVTILVISTADKATKYELEMKTDSAVVPLTLAQ
                     RKYAVQSKAGYFNLYEAMGGEGSMAKWAEEVPPMANKDYTHFNYKGAQKVAGLLYDQI
                     QTGYAQYKLMRKNKKVLPTKPTVDSVSSKNNTVNAQ"
     assembly_gap    5283..5382
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5383..6632)
                     /gene="argS"
                     /locus_tag="P5335_04005"
     CDS             complement(<5383..6632)
                     /gene="argS"
                     /locus_tag="P5335_04005"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRZ74076.1"
                     /translation="MNLEEFLNQPVQKAVQTIFDISPEKIEFQSTRKDFEGDITLVIF
                     PLLKLIKGNPTEIGNKIGTFLVENVVEVEKFNVVSGFLNIVISDSYYSKFFSEIKNNP
                     TFGFVSQNEKEKAMMVEYSSPNTNKPLHLGHIRNNLLGFSVAKIIKASGKKVYKTQII
                     NDRGIHICKSMLAWQKFGNGQTPESTGLKGDKLVGNFYVAFDKQYKAEINDLIAQGQK
                     EEEAKKNAPSILEAQQMLLDWEAGKPEVIELWKTMNRWVYDGFETTYKNLGVNFDSYY
                     YESNTYLLGKEVVQFGLEKGIFEKDPDGSVWIDLTEEGLDRKIVLRSDGTAVYMTQDI
                     GTAIQRVKDFPDVGGMVYTVGNEQDYHFKVLFLILKKLGFDWAKHLFHLSYGMVELPS
                     GKMKSREGTVVDADDLMDEMTETAA"
     gene            6894..>8242
                     /locus_tag="P5335_04010"
     CDS             6894..>8242
                     /locus_tag="P5335_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRZ74077.1"
                     /translation="MKTKLLFLLLLIPILGVSQAITVNTTTYTVPELVSNVLVNSPCL
                     TVNNINWSTGTNFGSSNGIGYFQNTNPNFPMQSGVILSTGNVMNSVGPNNSMLNDGLN
                     TWPGDSDLENILANAGIAMTSTNATVLEFNFTAMSPYFDFDFLFASEEYGNFQCQFSD
                     AFAFLLTNLNTGVTTNLAVVPGTNSPISVVTVRDFLYNSACPSVNPQFFGSFNGGANS
                     ALSPTNFNGQTVVMNASAVLVPNTPYKIKLVVADRIDPQSDSAIFLSANSFNIGQNVL
                     GDDLIIASNSALCSGESHIINSNLSATQYSFVWKKDGVVLVGENGPTLTITEPGSYEL
                     TYTNIAFPCQVVSDIVQIEYNSVFNAPDPIDILKCDSGASTYQFNLALNNPVVTAGIS
                     GGTTVTYHATLAQATNSTSALPLLYTSAGNQTIYVRVQKNNTSCYSIKSFQLLVTPAP
                     "
     assembly_gap    8243..8342
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8343..11635
                     /locus_tag="P5335_04015"
     CDS             <8343..11635
                     /locus_tag="P5335_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HRZ74078.1"
                     /translation="NTSCYSIKSFQLLVTPAPVAHQPSDLLGCARITDPTRGNFRLSN
                     LNESILNGQSASQNILTYHSSLENANTGVNPLTQVQYVSTNRVIYARLQNASDSSCYS
                     VVSFNIIVTPLPLVDTKDDVIVCDYYILPPLTNGNYYTGPNGTGIAKFAGDIIEETQR
                     IYIYNIGLIEPFCPNQSSFMVTIIKPEDINTTNNQFCNSYNVPNSAIGGYFTGPGGTG
                     TQLNGTTLTSSQTIYFYFVSPEPPYCILDLPINLTILPAPSTETFTNVFDCTSYTLPS
                     ISNGQYFDAPNGSGNQLAPGTIITETTTIYIYAANSFCESQTSFTVYIGIDAPTSTTE
                     CVNFILPSLPIGGYFTGPNGTGQQIQAGTVINSTQTIYVYAPSQSQPNCTDNLNFTVT
                     IALPVIETPLVTTGCESYFLPSIPLGNYFTGPNGTGTALAVGSEVTSSQTLYIYLNNG
                     NGCQNSVSFDITVLTPPQIDSRSDIDGCNNYVLTDLTLGHYYTGPNGSGNMLHGGDII
                     TTSQIIYIYAEENGCSAETSFQVNVFEIHADVLEDITVCDSYVLPTLTAGNIYYSAPD
                     GPNGFGTVLAPGSVITTTQTIYIYKENQIRPAFSCTDQSSFTVTVNISPVIAPISNVN
                     VCNSFILPSIAVGNYYTQSGGTGTMIVAGTELTTSQTLFIYAETGTTPNCFDEKSFDI
                     NIFNVDILDNVSTCSNYVLQPLTVGKYYRGPNGTGGQIPAGSSITTTQTIYIYALSGY
                     SPNCSDESSFIVTIVPTPIANTVPLVNRTVCDEDGENDGITPFDFTTLSSIVLGSQSG
                     SEFTVSYYASLSDASSQINSFSSSELQLVYVRVNNTLVPDCFDIKPITIIVNKIPEPT
                     PLDGIVCIESETGNLLNPYTIFSGLSSTTHTFEWYLDTTLIEGATGSSLQVMAPGVYS
                     VIATSLATGCSSEPTPAEVLASEPAAINFTVSMAFSSTNSITINANGVGGNYEYSLDG
                     SPFQDSPTFDNVASGIHSVTVRDKNGCGSATDTVLVINYPKFFTPNGDGYNDTWNIVD
                     LKEQVKATIYIYDRYGKFIAQLFPNKKGWDGLYNGQQMPSSDYWFTVTYEENNQTKEF
                     KSHFSLKR"
     gene            complement(11879..12547)
                     /locus_tag="P5335_04020"
     CDS             complement(11879..12547)
                     /locus_tag="P5335_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRZ74079.1"
                     /translation="MKSKRVQHTGQARLFESDILEMLTKTHPGIIWGVYIPILSYILF
                     RANQVYGLEIKTIVLLFFAGMIYWTFFEYIAHRFLFHMVSDNIKTQKVAYILHGNHHE
                     YPRDRQRLFMPLVPSLILATVLFLFHYLFLGKYNWAFFPGFMIGYLSYASMHYAIHAF
                     DPPFKFMQPLWRNHQLHHYKDEHLGFGVSNTFWDRVFGTMFDLTKNKQDKEKVKELMF
                     DKEN"
     gene            complement(12639..13649)
                     /locus_tag="P5335_04025"
     CDS             complement(12639..13649)
                     /locus_tag="P5335_04025"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRZ74080.1"
                     /translation="MYHSKIAGLGFYVPENIVTNDDLSKLMDTNDEWIQERTGIKERR
                     HVQRGDGNTTTSMGVKAAQIAIERSGVAKEDIDFIVFATLSPDYYFPGPGVLVQRDLG
                     LNTIGALDVRNQCSGFVYAVSVADQYIKTGMYKNILVIGSELQSYGLDMTDRGRGVSV
                     IFGDGAGAAVLSREEDLTSGILSTHLHSEGIHAEELAVVAPGMGKRWVTDIIKDNDPN
                     DESYYPYMNGQFVFKNAVVRFSEVINEGLQANNLSVSDISLLIPHQANLRISQFIQHK
                     FKLSEEQVFNNIQKYGNTTAASIPIALTEAWEQGKIKKGDIVVLAAFGSGFTWGSVII
                     KW"
     gene            13857..14483
                     /locus_tag="P5335_04030"
     CDS             13857..14483
                     /locus_tag="P5335_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74081.1"
                     /translation="MRFILPVLFYFILVPVTQAQFDNTNKSTSFGGNFGVTPSTTGSS
                     LFTPKSTPSPEPNTTPLREEKKIDLTQNYGLMEKKFEVKPNYLTGEVEMKEEYKRGKY
                     FGDFRSKSKFIKVMCRDHEAVDGDRVSIYLNDKLVQSSVYLTESFKVYFIELQPGFNN
                     LEFLALNQGESGPNTAQFAVQDEFGNVIMSDIWNLATGAKASLIVVKE"
     gene            14583..16988
                     /locus_tag="P5335_04035"
     CDS             14583..16988
                     /locus_tag="P5335_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRZ74082.1"
                     /translation="MKIQLLLFLLVSYFVSAQENLTYQKPPKEILELADYERAPTVNM
                     DTNKEYMLLSYRSTYKTLDELNQEEMRLGGLRINPVTNISSTVTYVNNLKLRKIADKD
                     EIQVKGLPENPKISNLSMAPNQKKIAFSHTTNIGVELWIIDVVTAQATKLTEAIVNAN
                     MGNPFSWFADSQSILVRMLVQNRPALIDVKKDLPTGPTVSTSDGNVSQNRTYQDLLKN
                     PLDEQNFETLVTSELYKISVNGKATLFKKADMYSGESISPDGNYIMISTIQRPFSYIV
                     PLSRFPQKTVVYDKTGNEIKVVNEVPLSEIIPKGFSSVRKGKRNMTWRTDKPATLYFV
                     EALDEGDQANKVDFRDEVFLWDAPFSSNPTSLVKTQQRFSGIEWGDDTTAIVYDQWYD
                     TRNMRTYLINPSSPTLSPKVISDRNYQDIYADPGDFETKKNQYGKYVLVLENNNAYLI
                     GKGFTKEGQFPFIDELNLTSLKTKRLYQSTYKDKKEDILSMEDVKKGEALVMIQSKNE
                     FPNYYFRNIKSKGKLTQITNFKNPFESIKNVYKEVIKYQRKDGVELSGTLYLPIGYDK
                     VKKEKLPLLIWAYPAEYKDKNSAGQSDKNENQFTFPNYGSFVYWVTRGYVVLDNASFP
                     IIGEGTTEPNDTFIEQLVANAEAAIDAVDKLGYINRKKVAVGGHSYGAFMTANLLTHS
                     NLFACGIARSGAYNRTLTPFGFQSEQRNYWDVPEVYNTMSPFMNANKMKTPLLLTHGE
                     ADNNPGTFTLQTERYFQALKNLGAPVRMVILPKESHGYAAKENILHLLWEQDQFLEKY
                     LKH"
     gene            complement(17456..18388)
                     /locus_tag="P5335_04040"
     CDS             complement(17456..18388)
                     /locus_tag="P5335_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRZ74083.1"
                     /translation="MLQVQNISFSYLNEITIDSLSFSLEKGKNLAVIGESGCGKSTVL
                     KLIYGLHDLDSGQIFWNDSEVLGPKFHLIPGMSYMKYLAQDFDLMPFITVAENVGKYL
                     SNFYPEEKKERIAELLEIVEMSEFATVKAKFLSGGQMQRVALARVLALEPEVLLLDEP
                     FSHIDNFRKNSLRRKLFAYLKEKKITTIVATHDSTDVLSFADDVLVMKKGQICTQGSP
                     NELYINPQSKYVASLFGDVNELQGHFLDSKDTQYLYPHQLKVTEVSKLKATVTHSYFR
                     GSHYLIEAIYENGVLFFEHEKELIPGYVLFLDRR"
     gene            complement(18513..19826)
                     /locus_tag="P5335_04045"
     CDS             complement(18513..19826)
                     /locus_tag="P5335_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRZ74084.1"
                     /translation="MKTLDKIPTNKIERAGQLVKTGIKVGGNYIAYYGEKMVNPSLNR
                     DKLNENNAEDIYDGLKNLKGSALKVAQMLSMDKNIMPRAYVEKFSLAQFSVPPLSAPL
                     VRKTFKNYLGKFPEQLYDTFTPDSINAASIGQVHKATKDGKNLAVKIQYPGVAESISS
                     DLALVKPFAVKMFNLQGKDSEKYFKEVEHKLLEETDYELELKQSLFLSKACANIENLR
                     FPKYYAELSSKKILTMDWMQGEHLSEFTAHNTDRAKGDQLGQALWDFYMYQMHHLRQV
                     HADPHPGNFLVDENANLIAIDFGCIKQVPEEFYIPYFELGRPEIIDNPVLFKEKLFEL
                     EILRTDDSPKEIAYFTGLFHQLLSLFTKPFHGDFFDFSDEEFFGAIAQMGEEFSKDTQ
                     LRKMNGNRGSKHFLYINRTFFGLYNLLHDLKARVHTQTFQKYIVN"
     gene            complement(19832..>20382)
                     /locus_tag="P5335_04050"
     CDS             complement(19832..>20382)
                     /locus_tag="P5335_04050"
                     /inference="COORDINATES: protein motif:HMM:NF036524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HRZ74085.1"
                     /translation="QENKIIESDFYSFYGSIDALKQDIWVKFFENVTTTIENDENYLT
                     YSNKNKLLTLYFSLFEVLTLNRSYVVFALKEDKNLLKNLHQLKEFRKHFKNFIAEIAD
                     TQTTEEDFKVNKLSKPLYVEGAWVEFLFLMKFWLDDTSKGFEKTDIMIEKAVKATFDV
                     LNTTPIDSLIDFGKFVWKEKFN"
BASE COUNT         6800 a         3481 c         3027 g         6774 t
ORIGIN      
        1 tttataaata atataaaaga atccataaaa tattcttaaa tttatattac taaaaattta
       61 attccaatga attatgaaaa aaattactct tttatttttt gcactaattt tagctgaagc
      121 aaatgctcaa gtagatgatg tttcgcctaa tttatcaaat aatagttttc cgaactatag
      181 ctctttattt aatccataca ctaccgaatt tttatatgat attggaagtg cgattggtac
      241 acaaggaaat gcaggcgttt tatttttaaa taatcagttt tgggtttctg cttgggcttc
      301 ttccaacatt catgtattaa ataattctgg tggatttgta gaaactttta ccgttgccgg
      361 ggttacaggt gcacgctctt taacaactga cggtactaat gtttatgtag gtgcaaataa
      421 cacaaacata tatgtaataa accctattac taagacatta attaatacta ttactaccac
      481 aactacatct ggaataaaca ttagattttt aacatatgat gccactttaa atggaaatgc
      541 aggtggattt tggattggta atttcaatac tgatattgtt gctgttgaca tgtccggaaa
      601 tcaaattaat gttataccgg caacaacaca tggtttaacc ggaatgtatg gagcagcagt
      661 aaatagtgct acaaatactt tatttgttta taatcaatct gctcctagta atgatatcat
      721 ttcaagtata tctttaagta ccggagcaat gttggtaaat tatgatgtct tcgcaaatga
      781 tttgtcatca aatggggtta cttcttcatt agcaggcgga acatttttgt cttcagacat
      841 agtgccgggt caaacgatat taattggagt ttctcaagcc actccatcaa atattctctg
      901 gggtataaat attaataatt tattatctac tactgattta gaaaaagatt catttaaaat
      961 atatccaaat cctgtaaaca acttactcca tttttctact acaaattttg atgaaattac
     1021 aatattcaat atagatggta aaataattaa atctttaaag tcaaattcat taaacgataa
     1081 aactttagat gtaagcaact tatcgaaagg cgtttatatt atctctgtta aaagtgaatc
     1141 taatataaaa tacttcaaaa ttgtaaaagt gtagtgtttt tataggaaga aattaatttt
     1201 taaaagaaga gtaagggaat tgctattact tttatctcaa caaataaatt tttatttcaa
     1261 tttttaaaac tgaaaataaa taaacggttg tggtcctgca gaagcnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnaatgt caataagatt tgtaaggttt tgataaaatg
     1441 gtttttaggt aatttgataa aattaccaaa gaaacgttca aaagctagtg ccaaaccgtg
     1501 aagagttccc caaaaaacaa atttccagga agcaccgtgc catagtccac ctaataacat
     1561 ggttaaaaat aaattaatat aggttctgaa ttttccattt cgatttccac ctaaagagat
     1621 ataaagataa tcccgcaacc atgtggataa tgaaatatgc caacgacgcc aaaactctgt
     1681 aactgaagcg gattgatatg gtgttctgaa attgggcgga agccaaaatc ccatcaataa
     1741 agccaagcca atcgccatgt cggaataacc cgaaaaatca caataaatct gaatggcata
     1801 tccataagat gccatcagat tttcaaatga agtataactg tttggagcat caaacactct
     1861 atctacaaag ttgatagaaa tataatcgga gatgaccgct tttttcaata atcctccaac
     1921 aatcaaaaac aaagctgcat taaattcttc ttttgtcaga tttagtcgct tgtatatttg
     1981 tggaataaaa tcactggcac gaacaattgg accggctact aattgaggaa agaaagaaac
     2041 gaaaaacaaa aagtccacaa aacttttggt aggttcaagt tgttttctgt aaacatcaat
     2101 cgtataactc agcgtttgaa aagtataaaa tgaaattcct acaggaagta tgatatcttg
     2161 aaactcaaaa tttctatcaa aaattaagtt cgtattatca ataaaaaaat tagtgtattt
     2221 aaaataacca agtaatccaa ggttaaccaa taaactgaca atcagataaa attttctata
     2281 aaaaatactt ttttcatcat aaattaattt ggcaataaca taatctaaaa cagaagaaaa
     2341 aataatcagt aaaaagaaaa ttccactaga tttataatag aaaaaaagcg aaaaagcgac
     2401 aacataagca atgcgtaatc gatgtgtttt ctgtgttaaa atataaaaga aataaaacac
     2461 caaaaacaag cctaaaaata aagcactatt gaataaaagt ggtttttgag gatcaaagac
     2521 aaaccaactt tgcacttgct cccaagtgag atgtccaaaa ttctgttcca cccaacggtt
     2581 tatattaaaa agcggtatca cttatttatt tctaatttat aattttcaaa tgaactcaaa
     2641 aaagcttcgg ttaaaagttg tccctgacgt tcataaccaa ctttggtata gtgtacttta
     2701 tctgctccaa ttattccttc ttttaccaat tgatttactc cgaaaagtcc accaaaattg
     2761 gaaaacatat cccaaacagc ataatttttc aagtcggctt gagctaaaat ttcttttgta
     2821 taatctgcca caaaagtatt aggaaatttt cgtttaaatt gtgaaggtgg cggagttgta
     2881 attaaaaata ctgtagttgg atttttagcg aaaataagtt ccatcatcaa attcagctga
     2941 actataaatt cggaggttat cattttatca aaactctcat tagttcctaa agaaatgatt
     3001 actaaatccg gttgtaaaac agctaattgt tcaaagaaca aaggaaattt agtgaaatca
     3061 gagcattttg caccgtttac tcctattgta tggtatataa ttcccggatt attattttct
     3121 aaaactaatc catttaaagc aaaatccgat tgattcaagg caggaattaa gtatatttta
     3181 tgtaataatg tatctgtttc aaacgaatga aagccctcct ctaaacgggt ttctaaagga
     3241 atgaattctg aacgttcaac tacttttttg gctttttctt tagacggtat tttcaatgtt
     3301 tttccggctc ggatattatt attttttaat ccgttttcct ttttaatttg agcaattgaa
     3361 acatcatact tttcagaaat gacggataaa ctttctccat ttttaatttt gtgcaaaata
     3421 tttttcggaa tttctgactc aattttaatc gtttttgagg ttaaagccac ctcaaacatt
     3481 tttatatttt gtggagtaat aatttttagt ttattaaaaa catattcttg attttttact
     3541 gtcatttcaa gagcaaaatc gttggattta gtaaacaggg ctattccact taaaccaacc
     3601 ggatttcccg tatttgggta aatatttcgt tggctttccc atgtttggtt ggaagaaaaa
     3661 cgaacatcag ccgttccatt tgttttaacc aaactatgag gaaaaacaag acctcgtccg
     3721 gcatttccaa attgttgctg caaattttta cgcaataatc ctgaaaacaa atcggcttga
     3781 atgtgtgaat cgccaatatg aacaacattt agctttccaa cttttgattt ttggagattg
     3841 accaattttt caaaaattgg gtttagaact ttattatttt ttatttcatt tattgccgta
     3901 ataacttcag ttgtatcaat ttcaacgaat aaagaatcag attgagcaca aagactaaaa
     3961 ctaaataaaa gaaaaaaaag aaaagaaaaa aatttattgt gcatttacgg tgttgttttt
     4021 agaagaaacg gaatcaaccg taggtttagt tggaagtact tttttatttt ttcgcattag
     4081 tttatattgt gcatatccgg tttgtatttg gtcatataat aaaccggcaa ctttttgagc
     4141 tcctttataa ttaaagtgag tataatcttt attggccatt ggcggaactt cttctgccca
     4201 tttagccatt gaaccttcac cccccatcgc ttcatataag ttaaaataac cggcttttga
     4261 ttgcacggca tattttcgtt gagccaaagt taagggaaca acggccgaat cggttttcat
     4321 ttccaattca tatttggttg ctttatcggc agttgaaatc actaaaattg tgacacccgg
     4381 aaaacattct ttaatatgat tgactactcg agtcatccct ttttcgtacc aattataatt
     4441 gtatgtacca taatttaaaa catttgttcc ataatgcaag atgattaaat cataatccaa
     4501 tcgctcttga aattgtttca ttaaattggt attaaaagta gaaattggca atcctgaatt
     4561 tccccgattt gaaaaattat caacatgaac acccttacca tcatcaaaat taaatccgta
     4621 aattggaata gaatcagctt gtttaaaaac tgctttaaaa cttttcaaat tagtcgttga
     4681 aatagctaaa gcatttagtt gtttctcggg atttagcttt ttaatcaaag tgtcttttcc
     4741 aattttaatt aacatttctc ccattttatt agaagaattt ccataaaaca aggttggatt
     4801 attaagttgg gaagtatgca ttccaaatcc ggctttgtaa cgtacccaag tatcagaaag
     4861 tgtatcatta gcaaaaaaaa catgcccatt aactccaaaa ggttttaatg gacgcttcac
     4921 attcagatac gattgtgttt tccaatttcc ggaatattga tgtgaaatag agcttctgga
     4981 accggctgat tcagaagtaa ttgaaacgaa tccaactcct tcacccccaa attgtgtttg
     5041 cattgccttt cgaaaatcct gtactattaa atctccatct gtcatagaat caccaaaata
     5101 agcaattctg atatctcctt cttgtttagt ttctaattgg tatagctttt caaaaaaagg
     5161 aattagatat tggaaacctt tgtattgtcc atattcctcc ggaggataag caattccttc
     5221 aatttgttca aaaactattt tttttcgata aaaagtatca atagtttttt catctgtttt
     5281 tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcagcagt ttcagtcatt
     5401 tcatccatca aatcatcagc atccacaacc gttccttcac gggatttcat tttacctgat
     5461 ggtaattcta ccattccata ggataaatgg aaaaggtgtt tagcccaatc aaatcctaat
     5521 ttttttaaga ttaaaaaaag tactttaaag tgataatctt gttcattacc aactgtataa
     5581 accattccgc cgacatcagg aaaatctttt acccgttgga tagctgttcc tatgtcttgt
     5641 gtcatataga cagcagtacc gtctgagcgt agcacgattt ttctatctaa tccttcctct
     5701 gtcaaatcaa tccatactga accatctggg tctttttcaa aaattccttt ttctaatcca
     5761 aattgaacta cttcttttcc gagtaaatag gtattacttt cataataata actatcaaaa
     5821 ttgacaccta aatttttata tgttgtttca aaaccatcat aaacccaccg gttcattgtt
     5881 ttccaaagtt caattacttc cggtttacca gcttcccaat cgaggagcat ttgttgagct
     5941 tctaaaatag aaggagcatt tttcttggct tcctcttcct tttgaccttg ggcaattaaa
     6001 tcatttattt cggctttata ttgtttatca aaagctacat aaaaattacc gactaactta
     6061 tcccctttta atccggttga ttcaggtgtt tgtccgtttc cgaatttttg ccaagccaac
     6121 atggacttac aaatatgaat tccgcggtca ttaatgattt gagttttata aactttttta
     6181 ccagaagctt taatgatttt tgccactgaa aagcctaata gattatttct tatatggcct
     6241 aaatgcaaag gtttattagt atttggagaa gaatattcca ccatcattgc cttctccttt
     6301 tcattttgag aaacaaatcc aaaagttgga ttgtttttaa tttcagagaa aaacttacta
     6361 taataagaat cagaaataac aatatttaaa aatccggaaa ctacattaaa tttttcaacc
     6421 tcaactacat tttcaactaa aaaagtccct attttatttc caatttctgt tgggtttccc
     6481 tttattaatt ttaataatgg aaaaataacc aacgtaatat caccctcaaa atcttttctt
     6541 gtagattgaa attcaatttt ttctggagat atatcaaata tagtttgaac cgctttttga
     6601 actggttgat ttaaaaattc ttctaaattc attattattc attatttcga caaagataat
     6661 tttaaattct aaaaacttta gatgcattct tattatttat gaataggttt tttgtctaaa
     6721 aaatcgtttt aaatgataaa acatctgatt ttatatttaa atttgtaagt attttatatt
     6781 attattaatt atttaaatgc atttcatcga ttttatttgc tttttatcga tatttattca
     6841 ttcatttgtt gtgtcaaaat tcaataaaag tttaaatacc caaattaata agcatgaaaa
     6901 caaaattact cttcttgttg cttttaattc caatacttgg agtttcacaa gctatcacgg
     6961 tgaacactac cacctatact gtacctgaat tggtttcaaa tgtgttagtg aattcacctt
     7021 gcttaacagt taacaatatt aattggagca caggtaccaa ctttggttca tcaaatggaa
     7081 ttggatattt tcaaaatacc aatcctaatt ttccaatgca aagtggtgtt atactgagta
     7141 ctggaaatgt aatgaattcc gtggggccaa acaactccat gcttaatgat ggattaaata
     7201 catggccagg tgacagtgat ttggaaaata ttctagccaa tgccggaatt gctatgactt
     7261 caacaaatgc aaccgtttta gagttcaatt tcacagcaat gtccccttat tttgattttg
     7321 actttttatt tgcttcagag gaatatggta attttcaatg tcagttttcc gatgcttttg
     7381 cctttttatt gacaaattta aatacgggag taacaactaa cctagctgtt gtaccgggaa
     7441 ctaatagccc tatttctgta gtaacagtta gagattttct atacaattct gcttgtcctt
     7501 ctgttaatcc acaatttttc ggttctttta atggaggtgc taattccgct ttatctccaa
     7561 ccaattttaa tggtcaaaca gttgtgatga atgcttcagc tgttttagtt cctaatacac
     7621 cctataaaat taaattagtt gttgcagata gaatagaccc acaatcagat tcagctatct
     7681 ttttatcagc caatagtttt aatattggac aaaatgtatt aggagatgat ttaataattg
     7741 cttctaattc tgcattatgt agcggagaat cgcatataat caatagcaat ttaagtgcta
     7801 cccaatactc atttgtttgg aaaaaggatg gtgtagtttt ggttggtgaa aacggaccca
     7861 ctttgaccat tacagaaccc ggaagttatg aattgactta tacgaatatt gctttcccat
     7921 gtcaagtggt aagtgatatt gtacaaatag aatacaactc tgtttttaat gctccagatc
     7981 ctattgacat tttaaaatgt gattcaggtg cttcaacgta tcaatttaat ttagcattga
     8041 acaatccggt tgtaacagca ggaatttccg gaggaacaac agtaacttat catgcaacat
     8101 tagcacaagc aactaattca actagtgcat tacccttact atacacaagt gccggaaatc
     8161 aaactattta tgtacgagtt caaaaaaata atacttcgtg ttattcaata aagtcatttc
     8221 aattattagt gactccggct ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnataatact tcgtgttatt caataaagtc atttcaatta ttagtgactc cggctccggt
     8401 agctcatcaa ccatctgatt tgttaggatg tgcaagaata actgatccca caagaggtaa
     8461 ttttagatta tcaaatttaa atgaatcaat attaaatggt caatctgcaa gtcagaatat
     8521 tctaacctat cactcttcat tagaaaacgc aaatactggt gttaatccat taacacaagt
     8581 acaatatgta agtacaaaca gagttattta tgcaagatta caaaatgctt cagattcttc
     8641 ctgttatagt gttgtttcat ttaacattat cgttacacca ctaccattag tagacactaa
     8701 agatgatgta attgtttgtg attattatat tctacctcct ttaacaaatg gcaactatta
     8761 tactggtcca aatggaacag gtattgcaaa atttgcagga gatattatag aagaaacaca
     8821 aagaatatac atttataata ttggtttaat tgaaccattt tgtccaaatc agtctagttt
     8881 tatggtaact atcataaaac cggaagatat caataccaca aataatcaat tttgtaattc
     8941 atataatgta cctaattcag caattggtgg atattttacc ggtccaggag gaacaggaac
     9001 acagttaaac ggcacaacat taacgtcatc tcaaactatc tatttttact ttgtctcacc
     9061 tgaaccacca tattgtatat tagatttacc aattaattta accatattac cagctccaag
     9121 cacagagaca tttacaaatg tttttgattg tacttcctat acattaccat ccatttctaa
     9181 tgggcaatat tttgatgctc caaatggatc aggtaaccaa ttggctccag gaactataat
     9241 tacagaaact actacaatat atatttatgc tgcgaatagt ttttgtgaaa gtcaaacaag
     9301 ttttacagta tatattggca ttgatgcacc aacatctact accgaatgtg ttaatttcat
     9361 attaccttct ttaccaattg gcggttattt tacaggtcca aatggaactg gtcaacaaat
     9421 acaagctgga acagtaataa acagtacaca aacaatatat gtatatgccc ctagtcaatc
     9481 tcaaccaaac tgtacggata atttaaattt tacagtaact attgctttac ctgtgattga
     9541 aactccactt gtaacaacag gttgtgaaag ttatttttta cctagtattc cgcttggaaa
     9601 ttattttact ggtccaaatg gaacaggaac agctttagca gttggtagtg aagtaacttc
     9661 ctctcaaaca ttatatattt atttaaataa tggaaatggt tgtcaaaact cggttagctt
     9721 tgatattaca gtcttaactc cgcctcaaat agactctagg tcagatattg atggttgtaa
     9781 caattatgtt ttaactgatc ttacacttgg ccactattat acaggaccaa atggaagtgg
     9841 aaatatgctt catggaggtg atataattac aacttctcaa ataatttata tttatgccga
     9901 agaaaatgga tgtagtgctg aaactagttt tcaagtaaat gtttttgaaa ttcatgctga
     9961 cgttttagag gacattactg tatgtgatag ttatgtatta ccaactctaa ctgccggtaa
    10021 tatttattac tctgctcctg acggacctaa tggttttggg acagtattag caccaggtag
    10081 tgttattaca acaacacaaa caatttatat ctacaaggaa aatcaaatca gacctgcatt
    10141 ttcatgtact gaccaatctt catttacagt tacagtcaat atttctccag taattgctcc
    10201 tatttcaaat gttaatgtgt gtaattcatt tatattgcct tctatagctg ttggaaatta
    10261 ttatacccaa tccggcggaa ctggaacaat gatagtagca ggaacagaat taacaacttc
    10321 acaaacatta tttatttacg cggaaaccgg cacaacacca aattgttttg atgaaaaaag
    10381 ttttgatata aatatattta atgttgatat tttagataat gtgtcaactt gttctaacta
    10441 tgttttacaa cctttaactg ttggaaaata ttatagagga ccaaacggaa caggtggtca
    10501 aattccggcc ggaagttcaa ttacaacgac tcaaacaatc tatatttatg ccttgtccgg
    10561 ttattcacca aattgttcag atgaaagttc tttcattgtt acaattgttc ctactccaat
    10621 tgcaaatact gttccgttag ttaacagaac tgtttgtgat gaagatggtg aaaatgatgg
    10681 aataaccccc tttgatttta ccactttaag tagtattgtt ttaggttctc aatccggaag
    10741 tgaatttact gttagctatt atgctagttt gagcgatgct tccagtcaaa tcaactcttt
    10801 tagttcctct gaattacagt tggtttatgt aagagtaaat aatacattgg tacctgattg
    10861 ttttgatatc aaaccaatta ctataattgt caataaaatt cctgaaccaa ctccattaga
    10921 tggtattgtt tgtatagaaa gtgaaacagg aaatctatta aatccatata ccatttttag
    10981 cggtctatcc tctactactc atacatttga atggtattta gatacaacat taattgaagg
    11041 tgcaacagga agttctttac aagtaatggc ccctggtgta tatagtgtaa ttgcaaccag
    11101 tttagcaaca ggatgctcgt ctgaaccaac tcctgcagaa gtgttagctt cagaaccggc
    11161 tgctattaac tttactgtaa gcatggcttt tagtagcact aattctataa ccataaatgc
    11221 aaacggtgta ggtgggaatt atgaatattc attagatggc agtccatttc aagacagtcc
    11281 tacgtttgat aatgttgctt ccggtattca ttctgttact gttcgtgata aaaatggttg
    11341 tggatctgct acagacactg ttttagtaat caactatcct aaattcttca caccaaatgg
    11401 agacggatac aatgacactt ggaatatagt tgatttaaaa gaacaagtta aagccactat
    11461 ctatatttat gatcgatatg gtaaatttat tgcccaatta ttcccgaaca aaaaaggttg
    11521 ggatggatta tacaacggtc aacaaatgcc ttcttctgat tattggttta cagtaactta
    11581 tgaagaaaat aatcaaacca aagaattcaa atctcacttt tcattgaaga gatagtttta
    11641 tattgttttg ttgtttagaa attaaagacc ttccacgtga aggtcttttt ttttacctat
    11701 aacattttga tctatttgat ttcttttggt tatatcacct ttctgcaaat caaagttatc
    11761 tttcatttaa atctcaatta ttatgactaa cgaatagaaa agactacaaa caattgtaag
    11821 ataattttaa tactcataaa aaaagccgcc taaagttttt atttagacgg cttctttttt
    11881 aattctcttt atcaaacatt aactctttta ctttttcctt gtcttgtttg tttttagtta
    11941 aatcaaacat agttccaaaa acacgatccc aaaatgtatt actcactcca aaacccaaat
    12001 gttcatcttt ataatgatgc aattgatgat ttctccacaa aggttgcata aatttaaagg
    12061 gaggatcaaa agcatgaatg gcataatgca tcgaggcata ggaaagatag ccaatcataa
    12121 atcccggaaa aaaagcccaa ttatatttac ctaaaaaaag ataatggaat aaaaataaga
    12181 cagttgctaa aatcaaactg ggaaccaaag gcataaataa tcgttgtctg tcgcggggat
    12241 attcatggtg atttccgtgc aaaatataag ctactttttg tgtttttata ttatcactaa
    12301 ccatgtgaaa aagaaatcgg tgagcaatat attcaaaaaa agtccaataa atcattcctg
    12361 caaaaaacaa caatacaata gttttaattt ctaatccata aacttgatta gccctaaaaa
    12421 gtatataact tagtattgga atataaacac cccaaataat tcctgggtga gtttttgtca
    12481 acatttctag aatatcactt tcgaaaagtc tggcttgacc agtgtgttgc actctcttgg
    12541 atttcatttt taaaaagttt aaaataacaa acaaaaacgc aaaattataa aaaagttaca
    12601 tactatagaa taccgataaa ttattattaa caactatttt accattttat aatcacactt
    12661 ccccaagtaa aaccactacc aaatgctgct aatacaacaa tatctccttt ttttattttg
    12721 ccttgctccc aagcctcagt taaggcaatt ggaatagatg ctgcagttgt atttccgtat
    12781 ttttgaatat tattaaacac ttgttcttct gataatttaa atttgtgttg aataaattga
    12841 gaaatcctca aatttgcttg atgaggaata agcaaactga tatcagaaac tgataaatta
    12901 ttagcctgta aaccttcatt aattacttca ctgaatcgaa caactgcatt tttaaataca
    12961 aattgcccat tcatgtaggg ataataactt tcatcattag gatcattatc tttaataata
    13021 tcagtcaccc agcgtttccc catcccgggt gcaactacag ctaattcttc tgcatgaatc
    13081 ccttcagaat gtaaatgagt ggaaagaatt ccgctggtta aatcttcctc tctggaaagt
    13141 actgctgctc ctgccccatc tccaaaaata acggaaacac ctctccctct gtcagtcatg
    13201 tctaatccat acgattgcaa ttcagagcca atcactaaaa tatttttata cattcctgtt
    13261 tttatatatt ggtccgcaac agaaacagca taaacaaaac cggaacattg atttcgtaca
    13321 tccaaagctc caatcgtatt taaccccaaa tctcgttgca ctaaaacacc cggtccagga
    13381 aaataataat ccggacttaa agtggcaaaa acaataaaat caatatcttc ttttgctaca
    13441 ccggatcttt ctatagctat ttgagcggcc tttacaccca tcgatgttgt tgtgttacca
    13501 tcacctcttt gaacatgtct gcgttctttt attccggttc gctcctgaat ccattcatca
    13561 tttgtatcca ttaattttga caaatcatcg ttggtcacaa tattttcagg cacataaaat
    13621 cctaatccgg ctatttttga atgatacata ttttgctttt ttaataaaat tagttacaca
    13681 aatttagcaa tcttttaaaa agtatcgcta aaaatgactt cgtttttaat aataaccaaa
    13741 ataacaactt cttcttgtta tttggcaccc ttattgttaa atataattta catttagcat
    13801 tttgttaaga gcctgattaa tgtctaacta gtatatttga aacgaaattt attaccatga
    13861 gatttatact gcctgtttta ttttatttta ttttggttcc tgtaacccag gcacaatttg
    13921 acaatacaaa taagtcaact tcatttggtg gtaattttgg agtgacacca agcacaaccg
    13981 gaagttcact cttcacacca aaaagcacgc cttcccctga acctaataca actcctctac
    14041 gagaagaaaa aaaaattgac ctaactcaaa attatggtct gatggagaaa aaatttgagg
    14101 taaaaccaaa ttacctcacc ggtgaagttg aaatgaaaga agaatataaa cgaggtaaat
    14161 attttggcga ttttagaagt aaatcaaaat tcattaaagt aatgtgccga gaccatgaag
    14221 cggttgatgg tgaccgagtt agcatttacc tgaatgataa attagttcaa agcagtgttt
    14281 acttaacaga atcattcaaa gtctacttta tagaacttca acccggattt aataatctcg
    14341 aatttttagc tttaaatcaa ggtgaatccg gtccaaatac agctcaattt gcggttcagg
    14401 acgaatttgg aaatgtaatt atgtccgaca tttggaattt agcgaccgga gctaaagcat
    14461 ctcttattgt agtaaaagaa taatacgctc ttctacttct tccctatttt aacagatttt
    14521 ttagaagttt ggatttatta tgtgctattt ttagtggcac aaatatttca aacttaatct
    14581 ttatgaaaat tcaattactt ctgtttcttt tagtgagcta ttttgtttcg gctcaggaaa
    14641 atttaaccta tcaaaaacct cccaaagaaa ttttggaatt ggcagattac gaaagagctc
    14701 caacggtcaa catggatacc aataaagaat acatgttgtt gtcttacaga agtacttata
    14761 aaacattgga tgaattgaat caagaagaaa tgcgtttagg cggacttcgc attaatcctg
    14821 ttacaaatat ttcaagtact gttacttatg ttaacaacct gaaattgcga aaaattgctg
    14881 ataaagatga aattcaagtc aaaggtttac cggaaaaccc aaaaataagc aacctttcaa
    14941 tggcacctaa ccaaaagaaa attgcctttt cacacactac aaatatagga gtcgaacttt
    15001 ggattattga tgttgttaca gctcaagcaa ctaaattaac cgaggcaatc gttaatgcaa
    15061 acatggggaa tccatttagt tggtttgctg acagtcaatc tattttagtt cgaatgttag
    15121 ttcaaaaccg tcctgcctta attgatgtta aaaaagattt accaaccgga cctacggttt
    15181 ccactagtga tggaaatgtt tctcaaaaca gaacatacca agatttgttg aaaaaccccc
    15241 ttgacgaaca aaattttgaa acattggtta cctccgaatt atataaaatt tcagtgaatg
    15301 gaaaagccac gttattcaaa aaagctgata tgtattccgg agaaagcatt tcgcccgatg
    15361 gaaattatat aatgatttca acgattcaaa gacccttttc ctatattgta cctttaagca
    15421 gatttcctca aaaaacagtt gtttatgaca aaaccggaaa tgaaattaaa gttgttaacg
    15481 aagtgcctct ttcagaaatc attccaaaag gattctcttc tgttcgaaaa ggaaaaagaa
    15541 atatgacttg gagaactgac aaaccggcta ctttatattt tgttgaagca ttagacgaag
    15601 gtgatcaggc taataaagta gatttcagag atgaagtttt tttatgggat gccccttttt
    15661 catccaatcc aacttcatta gtgaaaaccc aacaacgttt ttcaggtatt gaatggggag
    15721 atgatacgac tgccatagtt tatgatcaat ggtacgacac cagaaacatg agaacctatt
    15781 taattaatcc atctagccca acactttctc caaaagttat atcggataga aattatcagg
    15841 atatttatgc agacccgggt gattttgaaa ccaaaaaaaa tcaatatgga aaatatgttt
    15901 tagttttaga aaacaataac gcttatttaa ttggaaaagg atttactaaa gaaggccaat
    15961 ttcctttcat tgatgagttg aatttaacct ctttaaaaac aaaacgttta tatcaatcta
    16021 cctataaaga taaaaaggag gatattttat ctatggaaga tgtcaaaaag ggagaagcat
    16081 tggttatgat tcaatcaaaa aatgaatttc ctaattatta cttcagaaat ataaagtcaa
    16141 aaggaaaatt aacacaaatt acaaatttta aaaatccctt tgaaagtatc aaaaacgtct
    16201 ataaagaagt aataaaatac caacgaaaag atggtgtaga gctttcagga acgctatacc
    16261 ttccaattgg atatgacaaa gtcaaaaaag aaaaacttcc gctcttaatt tgggcctatc
    16321 cagctgaata taaagacaaa aattcagcgg gacaaagcga taaaaatgaa aatcaattta
    16381 cctttcctaa ctatggctct tttgtttatt gggttacaag aggctatgtt gtgttagaca
    16441 atgcttcttt cccaattatt ggagaaggta caacggaacc aaacgacacc ttcatcgaac
    16501 aattagttgc caatgctgaa gcagcgatag atgccgtgga taaattgggg tatatcaacc
    16561 gtaaaaaagt agcggttggc ggacattctt acggagcatt tatgacggca aatttattga
    16621 cacattctaa cttatttgct tgtggaattg ctcgtagtgg tgcctataac agaaccttaa
    16681 cgccatttgg atttcaaagt gaacaacgaa attattggga tgttcctgaa gtttataaca
    16741 cgatgtcgcc gtttatgaat gctaataaaa tgaaaacacc tttattatta actcacggtg
    16801 aagctgataa taatccggga acatttactt tgcaaacgga acgttatttt caagcgttga
    16861 aaaacctagg tgctcccgtt cgcatggtga ttttaccgaa agaaagtcat gggtatgctg
    16921 caaaagaaaa tattcttcat ttactttggg aacaagatca gtttttggaa aaatatttga
    16981 agcattaagt ttaagaatat aaaaaaacaa aatagttgaa ccctgaaatt aataaatttt
    17041 agggtttatt tttttacaga aattgagggt tcgtattttt cttttgaaaa tacattttgg
    17101 aaattatatg aattgctagg agttgtaacg cagacttgat tagtaatttt ttgaaaataa
    17161 aaatgtaaaa taaatgaatt tcactattca tattgttgac attttaattg agcagtgctg
    17221 aaggaaagac ttcgaagttt aatttatttc atgtttttga agcaccacaa cccttaagta
    17281 tcccttcttt attccttaag tatcccttac tttgactcca ctgaaattta tataattttt
    17341 gtattttcta tattgaattt ttagtcacta atttattttt gattcaccat atcattcttt
    17401 ctattttcct taattcgatt ggaacaatct ttatttgaaa tatattgttt tagttttatc
    17461 ttctatccaa aaacaataca taacccggaa tcagttcctt ttcatgttca aaaaacaaaa
    17521 caccattttc atagattgct tcaatcaaat aatgactacc tctgaaataa ctatgggtaa
    17581 ctgttgcttt taattttgac acttcggtga ctttcaattg gtgtggatat aaatactgtg
    17641 tatctttcga atccaaaaaa tgtccctgta gttcattcac gtctccaaaa agtgaggcaa
    17701 catatttaga ttgaggattt atatacaatt catttggact gccttgagtg caaatttgtc
    17761 cctttttcat caccaaaaca tcatcagcaa atgacaaaac atcagtacta tcatgtgtag
    17821 ctacaatcgt ggtaattttt ttctctttca aataggcaaa caatttacga cgcagactat
    17881 tttttctaaa attatcgatg tgactgaaag gctcgtctaa caataatacc tccggttcca
    17941 aagccaaaac acgagccaaa gcaacccgtt gcatttgccc accgcttaaa aatttggctt
    18001 tgacagttgc aaattcgctc atttcaacaa tctctagcaa ttcggcaatc cgctcttttt
    18061 tttcttccgg ataaaaatta gagagatact tcccaacatt ttctgcaacg gtgataaaag
    18121 gcatcaagtc aaaatcctgt gccaaatatt tcatgtacga cattccggga atcaagtgaa
    18181 acttcggacc taaaacttct gaatcgttcc aaaatatttg tccgctgtct aaatcatgta
    18241 agccgtaaat taacttcaga acagtacttt ttccacatcc actttcgcca atcacggcta
    18301 gattcttgcc tttttcaagt gaaaaagaaa gggagtcaat cgtaatttca ttgagatagg
    18361 aaaaggaaat gttttgtact tggagcatgt atctgttgaa aatgaatttg aacaaagata
    18421 atttaaaaaa aaataagagc ggcaaaactc aaacgtggag tgccgctctt accaacctaa
    18481 ccaataaata aacaaactac atttcaacta aatcagttga caatatattt ttgaaacgtt
    18541 tgtgtgtgga ctcttgcttt caaatcatgt aacaaattat acaaaccaaa aaacgttcgg
    18601 ttgatgtata aaaaatgttt agaacctctg ttaccattca tctttcgtaa ctgcgtgtct
    18661 tttgaaaact cttcgcccat ttgagcaatg gctccaaaaa attcttcatc tgaaaaatca
    18721 aagaaatctc catgaaacgg tttggtaaac aggctcaata attgatgaaa caatccggtg
    18781 aaataggcaa tttctttggg tgaatcatcg gttcgtaata tttctaattc aaataatttc
    18841 tctttaaaca acacaggatt atcaataatc tcaggtcgtc ccaattcaaa atacggaata
    18901 taaaactctt ccggtacttg cttgatgcat ccaaaatcaa tcgcaattaa attagcattt
    18961 tcatcaacca aaaaattacc gggatgcgga tcggcatgca cctgacgcaa atgatgcatt
    19021 tgatacatat aaaaatccca caaagcttgt ccgagttgat ctcctttggc tcgatcggtg
    19081 ttatgagccg taaattccga taaatgttcg ccttgcatcc aatccatcgt caaaattttc
    19141 ttcgaggata attcagcata atactttgga aaacgtaaat tttcaatgtt tgcacaagcc
    19201 tttgaaagga acaaactttg cttcaattcc aattcataat cggtttcttc caataatttg
    19261 tgttctactt ctttgaaata tttctcagaa tcttttcctt gaaggttgaa cattttaacg
    19321 gcaaatggtt ttaccaaagc caaatcagaa ctaatacttt ccgccacgcc gggatattgg
    19381 attttcacag ctaaattttt cccatctttc gttgctttgt gtacctgacc gatacttgca
    19441 gcatttatag aatccggcgt gaaggtgtcg tatagttgtt ccgggaattt ccctaaataa
    19501 tttttaaatg tttttcgaac caatggagca ctcaaaggcg gcactgaaaa ttgtgccaat
    19561 gaaaattttt ccacataagc tctgggcatg atgttcttgt ccatgcttaa catttgtgct
    19621 acttttaaag cactgccttt taagtttttt aaaccgtcat aaatatcttc tgcattgttt
    19681 tcattcaact tgtcgcggtt caaactcgga ttcaccattt tttcaccgta gtaagcaata
    19741 tagtttccgc cgactttaat tccggttttg actaattgac ctgcacgttc aattttgttg
    19801 gtgggtattt tatctagtgt tttcaatggt tctaattaaa tttttctttc caaacaaatt
    19861 tcccaaaatc aattaaactg tcaatgggtg tcgtatttaa tacatcaaaa gttgctttta
    19921 ccgctttttc tatcatgata tcggtttttt caaatccttt cgaagtatca tctaaccaaa
    19981 atttcatgag gaataaaaac tctacccaag ctccttctac atacaacggt ttagataatt
    20041 tgttgacttt aaaatcctct tccgtcgttt gtgtatctgc tatttctgca ataaaatttt
    20101 taaaatgttt tctaaactct ttcagttgat gaagattttt caatagattt ttgtcttctt
    20161 tcaaggcaaa aactacataa cttctgttta aagtcaacac ttcaaacagt gaaaaatata
    20221 aagtcaacaa cttattttta ttggaataag tcaaataatt ttcatcgttt tcaatggtag
    20281 ttgttacatt ttcaaaaaac tttacccata tatcctgctt caaagcatca atcgaaccgt
    20341 agaacgaata aaaatcactt tcaataattt tgttctcttg gc
//
LOCUS       DAOTCW010000030        20242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1431_length_20242_cov_50.058701, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000030 DAOTCW010000000
VERSION     DAOTCW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 20242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20242
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1431_length_20242_cov_50.058701"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..632
                     /locus_tag="P5335_04055"
     CDS             72..632
                     /locus_tag="P5335_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 periplasmic binding fold superfamily
                     protein"
                     /protein_id="HRZ74086.1"
                     /translation="MKNLKMMAFAITLITLTSCSNDDSPVNEEELITTVITTLTGGGQ
                     TITLTARDLDGDGPNAPVITVSGDLVAGTTYAGATRFLNELNNPVDEITEEVQEEGAE
                     HQVFYQLASNIGTVTYHSANTDANGNPIGLQFTLTTSANNATSGTFVVTLIHLPNKTA
                     TGVAAGDITNAGGATDALAIFPLTIL"
     gene            773..1147
                     /locus_tag="P5335_04060"
     CDS             773..1147
                     /locus_tag="P5335_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HRZ74087.1"
                     /translation="MNSRFTYLMRLLLGVILLVFGLNKFLNFIPAPELPENAASFITS
                     LATTGYVLQVVGVLEILIGLLLLTNKWVAFALVVLAPISVNILLFHLFLDIPGISGAL
                     LVAIINGILMYKLWPKYKPLFN"
     gene            1214..3310
                     /locus_tag="P5335_04065"
     CDS             1214..3310
                     /locus_tag="P5335_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRZ74088.1"
                     /translation="MFAIVCVISVNAQPTSSSFAYPETKKEVVKDTYFGTEVSDPYRW
                     LEDDRSAETGAWVKAQNEVTFGYLSQIPFRNQIKERLEKLWNYERISAPFKEGDYTYF
                     YRNNGLQNQSVIYRKDKSGKEELFLDPNTFATDGTTSLADISFSKDGSLMAYSISEGG
                     SDWNKIIIMDAVSKKVLESPIVDVKFSGIAWLGNDGFFYSSYDKPEGSELSAKTDQHK
                     LYYHKLGTTQKEDKIVFGLNEKRRYVSGAISEDQHYLIITAANSTTGNELYIRDLNDP
                     NSKLETIVANFDSNNYIIDNIGTKLFIVTNLNAPNQKVVTVDFKNPTPENWKNCIAET
                     DYVLSPSTGAGYLFANYMEDAISQVKQYDYDGKLVREISLPGIGSAGGFGGKKTETEL
                     YFSFSNYLTPGTIYSFDPKSGTSKVYEKPKVDFNSDNYESKQVFYTSKDGTKIPMIIN
                     HKKGLKLDGKNPTILYGYGGFNVSITPNFSVANAVWMENGGIYAVANLRGGGEYGKKW
                     HDAGTQQKKQTVFDDFIAAGEYLIAQKYTSSDYLAIRGGSNGGLLVGAVMTQRPDLMK
                     VALPAVGVLDMLRYHTFTAGAGWAYDYGTANDSKEMFTYLKNYSPVHNVKKGVKYPAT
                     LVTTGDHDDRVVPAHSFKFAAELQDKQAGENPVLIRIDVNAGHGAGKPISKTIEENAD
                     VQAFTLYSMGIKELKK"
     gene            complement(3355..4032)
                     /gene="mtgA"
                     /locus_tag="P5335_04070"
     CDS             complement(3355..4032)
                     /gene="mtgA"
                     /locus_tag="P5335_04070"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRZ74089.1"
                     /translation="MIKKTLRFLWKAALWFFGISIFMVILYKFVPVPFTPLMAIRAVE
                     HKMAGKPMTCSHDWVPIEEISPNLQKAVIASEDANFLTHKGFDFKAIEKAMENNEKGK
                     KIKGGSTISQQTAKNVFLWQGRSYFRKGLEAYFTVLIELIWGKERIMEVYLNSIEMGD
                     GVYGAQEASMVWYHKDAKKLTRKEAAGIAAILPNPRKFKASNSSAYINRRKARISRYI
                     GYIELNY"
     gene            complement(4035..>4153)
                     /locus_tag="P5335_04075"
     CDS             complement(4035..>4153)
                     /locus_tag="P5335_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRZ74090.1"
                     /translation="KPIVKQLSEHVYCGVRLGGMGVAIGSIVGKELADLIEQ"
     assembly_gap    4154..4253
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4254..5308)
                     /locus_tag="P5335_04080"
     CDS             complement(<4254..5308)
                     /locus_tag="P5335_04080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRZ74091.1"
                     /translation="MQFSFWELKNWFTKVDYTIVGSGIVGLHCALQLRDKYPSAKILI
                     LEKGVLPQGASTKNAGFACFGSVSEIVDDLRSHTEEEVLQLVQKRWNGLQLLRRRIGD
                     EVLDLKPFGGYELFLKEEESTYHECLQQLPFINELLKPLFKNEVFSKEVNRFAFQGIQ
                     DCVLFNPYEAQIDTGNMIQYLLKLAIQQDVLILNQQTVTSYVEKREGVEVVLGDFSFS
                     TNKLLFATNGFAGEITKGEVKPARAQVLITKPIPNLEIMGTFHLDKGYYYFRNFEDRI
                     LLGGGRNLDFEGETTTSFDQTERIQNKLEQLLTSVILPNQAFEIEQRWSGIMGVGKQK
                     KPIVKQLSEHVYCGVRLG"
     gene            5535..8744
                     /locus_tag="P5335_04085"
     CDS             5535..8744
                     /locus_tag="P5335_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HRZ74092.1"
                     /translation="MKLKLLTIALVASVSFSFAQEKNSFWKASTLKSTSELLESKMQL
                     PQNNVFELDVIGIKNALSTSPQRFANSKGGTIISFPNAEGAMERFSVYENSIMAPELA
                     ANYPEIKSYIGIGIDNPTSTLYFSISPLGFKSMQLNAGKSAVFIEPLSADLTTYSVYK
                     KADKVKNFSPFECAVLDQVQNQIDPSALRPNADDSLLRTFRLAMSVTGEYTAYFGGTK
                     ALALAAINNTMTRVNGVFEKDFAVRMVLIANTDLVIYTNASTDPYSASSSMSNWNSQL
                     QSTLTSVIGEANYDVGHLFGATGGGGNAGCIGCVCVNGSKGSGYTSPADGIPSGDNFD
                     IDYVAHELGHQFGANHTFTFSNESGTGAQMEPGSGSTIMGYAGITTKDVQPHSDAYFH
                     AISIQQVTNNVKAKTCQTNTSTGNAVPTANAGLDYTVPKSTPFMLTGTGTDANGDVLT
                     YNWEQMDAGTSTTTTPSATKTAGPNFRSYNSSTSPTRYFPRMQSVLTGATTTTGTEIL
                     VEALPSVARTFNFRLTVRDNRAGGSGNNSDDMVVTVNATAGPFTVNSPNTAVSYAGGS
                     TQTITWAVAGTTANGVNCANVDILLSTDGGNNWNTLLAATPNDGTEAVTIPNTAGTQN
                     RIMVKGTNHIFFDVSNTNFTITAGSSDTVAPTAPTLVASGTTQTTTNLSWSGATDNVA
                     VTGYDIYRGTTLLGSTASTTYAVSGLTASTTYSFTVKAKDAAGNVSVASNAVSVTTLA
                     PVADTTAPTAPTLSASGTTQTTTNLSWSGATDNVAVTGYDVYRGTTLLGSTASTNYAV
                     SGLTASTTYSFTVVAKDAAGNVSVASNAVNVTTLAVSTVTYCASQGNSTADEYINRIQ
                     LGTINNLSGNNSGYGNFTALSTNLVRGTSNSITITPAWTGTVYSEGYRVWIDYNRDGD
                     FADSGEQVVNVTKTKTTPITRSFTVPTTALIGSTRMRVSMKYNASPTYCETFSYGEVE
                     DYTVNIVTTARGEQEIVLEPVSKESFTLYPNPIKGDLVHIDAETQFTTYKILNLLGQV
                     IAQGKIENKTVLVSNLASGTYLLEVSDNETSAVKRFIKQ"
     gene            8835..10745
                     /locus_tag="P5335_04090"
     CDS             8835..10745
                     /locus_tag="P5335_04090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRZ74093.1"
                     /translation="MKKIILILAFIMGASYVSAQQILTPELLWKLGRVSALGISKDGK
                     NVIYKVSLPSVEENKSDSKFYSISVNGGFATEIPETKELLTDKNLSPDGKYLLSNKEV
                     KLEKILGKDLYPELEKSNVQVYNGLDYRHWDTWNDGNHNHVFYADANGNDKSVAIDIM
                     KGEPYDSPQKPFGGDEDFTWSPDGKSIIYVCKKKSGTAYATSTNTDLYEYNLENKTTK
                     NLTESNLGYDTNPLFSPNGDLTWLQMKRDGYEADKNDIIVRFKGMDMNLTANWDGTVD
                     GFKWSSDSKKIYFTAAVDGTKQLFEVNFPGLTRKAVMVRQITTGTFDVNDLVGFTGDK
                     IIVTRTDMNHSKEIYSYDLKKNAWLKLTEVNDKAYAKLSLPTYEKRYVTTTDGKKMLV
                     WVILPPNFDKTKKYPTLLYCQGGPQSALTQSYSFRWNFSLMASQGYVIVAPNRRGMPG
                     HGVAWNEQISKDWGGQVMDDYLSAIDDVSKESYVDKSRLGAIGASYGGYSVFYLAGIH
                     NKRFKTFISHCGVFNLQSMYGTTEEVFFTDWDSGGPYWEKDNAVAQKTYTQFNPINLV
                     SKWDTPILIIQGGKDYRVPIGQGQEAFQAAQLRGIKSRFMLFPEENHWVLKPQNALVW
                     QREFFGWLKETL"
     gene            complement(10801..12147)
                     /gene="accC"
                     /locus_tag="P5335_04095"
     CDS             complement(10801..12147)
                     /gene="accC"
                     /locus_tag="P5335_04095"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRZ74094.1"
                     /translation="MFKKILIANRGEIALRVIRTCKEMGIKTVAVYSTADAESLHVKF
                     ADEAVCIGPPPSNLSYLKMSNIIAAAEITNADAIHPGYGFLSENAKFSKICQEHGIKF
                     IGASPEMIDRMGDKASAKATMKAAGVPCVPGSEGLLESFEQAAQIAKDMGYPVMLKAT
                     AGGGGKGMRAVWKADELQKAWESARQESAAAFGNDGMYMEKLIEEPRHIEIQVVGDAY
                     GKACHLSERDCSVQRRHQKLTEETPSPFMTDELREKMGEAAVRAAEFIKYEGAGTVEF
                     LVDKNRNFYFMEMNTRIQVEHPITEQVIDYDLIREQILVAAGVPISGKNYLPQLHSIE
                     CRINAEDPYNDFRPSPGKITVLHAPGGHGVRLDTHVYAGYSIPPNYDSMIAKLITTAQ
                     TREEAISKMKRALDEFYIEGIKTTIPFHRQLMDEPDYVAGNYTTKFMESFKMNDPE"
     gene            complement(12247..12735)
                     /gene="accB"
                     /locus_tag="P5335_04100"
     CDS             complement(12247..12735)
                     /gene="accB"
                     /locus_tag="P5335_04100"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRZ74095.1"
                     /translation="MDIREIQNLIKFVAKSGATEVKLEMDDFKITIKTTEAGAAEASY
                     FQHYPAAQPMQQMMPQHQMPQPVAPVTPVAAPAVDDNSKYITIKSPMIGTLYRKPAPD
                     KAPFVEVGATISKGDPVCVIEAMKLFNEIESEVSGKIIKILVDDMSPVEFDQPLFLVD
                     PS"
     gene            complement(12763..>12864)
                     /locus_tag="P5335_04105"
     CDS             complement(12763..>12864)
                     /locus_tag="P5335_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HRZ74096.1"
                     /translation="ETKLKKGDNLIFAAFGGGFTWGAIYLKWAYDKK"
     assembly_gap    12865..12964
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12965..13342)
                     /locus_tag="P5335_04110"
     CDS             complement(<12965..13342)
                     /locus_tag="P5335_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HRZ74097.1"
                     /translation="MSKITAAITAVGAYVPDFVLSNKVLETLVDTNDEWITSRTGIKE
                     RRLLKEEGKGTSFLAIKAAQNLFEKNNINPEEIDLVIMATATPDMPVASTGVYVASQI
                     GAVNAFAYDLQAACSSFLYGMSNA"
     gene            complement(13507..13701)
                     /gene="rpmF"
                     /locus_tag="P5335_04115"
     CDS             complement(13507..13701)
                     /gene="rpmF"
                     /locus_tag="P5335_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRZ74098.1"
                     /translation="MAHPKRKTSKTRRDKRRTHYKATVAQIATCPITGEAHLYHRAYW
                     HEGKMYYRGQVVIDKTEAVA"
     gene            complement(13711..14250)
                     /locus_tag="P5335_04120"
     CDS             complement(13711..14250)
                     /locus_tag="P5335_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRZ74099.1"
                     /translation="MKVSNEFLIPFIGLKLGKHQFDYQIDKTFFDRFEYDDFESCDIK
                     TTVVLEKKTTMLELHFKHKGTVNVPCDLTSEPFDLPIKGKIKLIVQFGDAFNDENEEL
                     LILPHGEHQVDISQYIYEMIVLSIPLKRVHPGVKDGTLQSEAIDKLQELTVKEKKVEK
                     KTEETDPRWDQLKKLLTDK"
     gene            complement(14335..15384)
                     /gene="pdxA"
                     /locus_tag="P5335_04125"
     CDS             complement(14335..15384)
                     /gene="pdxA"
                     /locus_tag="P5335_04125"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRZ74100.1"
                     /translation="MVKKAENILVGISIGDLNGIGSEVVLKTFEDPRMLELCTPVIFA
                     NVRMLSFLRKSFESTALLHGIDKLDQIVLGKINVLNLWRDSFNLEYGVADDIVGSYAI
                     KSFVAATKALKEGEIDVLVTAPINKYNIQSEEFKFPGHTDYLNQELEGDALMLMVNDS
                     LRVGLFTDHIPVTEVSKYLTEKLIRQKIATVKQSLMQDFKISKPKIAVLGLNPHCGDH
                     GIIGKEDDEVIRPTIKKLFDEGTFVFGPYAADSFFGNNSQEKFDAVIACYHDQGLIPF
                     KTLSFGNGVNYTAGLNKIRTSPDHGTAFEIAGKNQADFNSFKEAVYLALDIYKSRMEY
                     AQITEKPLKTKEKQF"
     gene            15442..16032
                     /locus_tag="P5335_04130"
     CDS             15442..16032
                     /locus_tag="P5335_04130"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRZ74101.1"
                     /translation="MFTGIIETLGIIKDIQREGNNLHVSVSSSITSELKIDQSLAHNG
                     ICLTVVAIKNDVYKVTAIKETIDKTTIGNWKIGDLINLERAMKLDARLDGHLVQGHVD
                     QIGVCNAVKESNGSWYFTFEYDSKPNNITIEKGSITVNGVSLTVVNSKQKEFSVAVIP
                     YTFEHTNFQTFKVGTKINLEFDVIGKYVAQLYEYKK"
     gene            complement(16547..16620)
                     /locus_tag="P5335_04135"
     tRNA            complement(16547..16620)
                     /locus_tag="P5335_04135"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16584..16586),aa:Ile,seq:cat)
     gene            16773..17129
                     /locus_tag="P5335_04140"
     CDS             16773..17129
                     /locus_tag="P5335_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HRZ74102.1"
                     /translation="MRKIYYLKTCDTCKRIIQSLPKTDGFLFQDIKEEPITVKQLEEI
                     HVLAGSYEILFSKRAKLYKEMGLKDENLSESDFKRYILEHYTFLNRPVIVIDKTIFIG
                     NSPKTVQAAIDFLTNE"
     gene            17122..18009
                     /locus_tag="P5335_04145"
     CDS             17122..18009
                     /locus_tag="P5335_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRZ74103.1"
                     /translation="MNKRNWALVAAMLVSIIYGITFTIAKDVMPKYVEPFGFILLRVG
                     GSVFLFWLVSFFGPKEKIALVDFPRIVAAAFFGVAFNMLTFFKGLSYTSPIMGAVLMV
                     TTPMIVLILSAIIMKERMKKRKIFGIVLGLAGTISLILYGKSLINAPNEMLGNLLVFV
                     NAVSYGFYLIIVKKLMDKYNAFTFVKWIYTFGFLMVIPFGWGEFNAVNWAIIPTDIYW
                     KIGFVVVFSTFLTYLLNLVSMRELKPTTVAVFIYLQPLFATVFAISLGKDDLSWIKIG
                     SAILIFTGVYLVTQKKSTQ"
     gene            18104..18964
                     /locus_tag="P5335_04150"
     CDS             18104..18964
                     /locus_tag="P5335_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRZ74104.1"
                     /translation="MIQSMTGFGKASLQLPTKKITVEVKSLNSKGLDLNVRMPSVYRE
                     MELGLRNTIAQKLERGKVDFSIYIEVTGEETSSKINVPIVKGYINQMKAVIPNADETE
                     LMKMAVRMPDALKTERDEIDENEWKEIMKVVDDAMKNIASFRLDEGKSLENEFVNRIE
                     NIRNYMNQAVSMDTERIETVKNRLRTALDELEANVDENRFEQELIFYLEKYDITEERV
                     RLENHLNYFIETIAGTEANGRKLGFITQEIGREINTMGSKSNHSEMQKLVVMMKDELE
                     KIKEQVLNVL"
     gene            19008..19595
                     /gene="gmk"
                     /locus_tag="P5335_04155"
     CDS             19008..19595
                     /gene="gmk"
                     /locus_tag="P5335_04155"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRZ74105.1"
                     /translation="MNKGKLLVFSAPSGSGKTTIVRHLLAQSDLNLEFSVSCTTREPR
                     GEEVHGKDYYFISLKDFKTHIKAEEFVEWEEVYRDNFYGTLKSEVERIWAKGKNVIFD
                     IDVAGGLRIKSKFKQETLAVFVKPPSIDELKIRLKKRSTESEDKINMRIAKASVELAT
                     APQFDKIIKNYDLEVAKAEAYELVKEFICQKNPND"
     gene            19607..20188
                     /gene="nadD"
                     /locus_tag="P5335_04160"
     CDS             19607..20188
                     /gene="nadD"
                     /locus_tag="P5335_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRZ74106.1"
                     /translation="MKIGLYFGTFNPIHVGHLIIANHMAEYSDLDQVWMVVTPHNPHK
                     QKSTLLDNYHRLEMVHLATKDYPKIKPSDIEFKLPQPNYTVNTLAHLQEKYPTYEFAL
                     IMGEDNLNSLHKWKNYEVILQNHDIFVYPRLNSGLIDEQFINHPKIHRVGAPVIELSS
                     TFIRESIKHGKNVRAVLPEHVWAFLDHNLFYRK"
BASE COUNT         6423 a         3574 c         3486 g         6559 t
ORIGIN      
        1 gtttttttgc ggatgaaacc ggaagaaaca ttcaactaca attaaaattt aattattaaa
       61 aatctaacaa aatgaaaaat ctaaaaatga tggcctttgc cataaccctg attaccctga
      121 cctcttgcag caatgatgat tctcctgtga atgaagaaga attgattacc acggtcataa
      181 ctacgttgac cggtggcggt caaacaatta ctttaactgc aagggactta gatggtgatg
      241 gtcctaatgc tcctgtgata accgtttcag gtgatttagt tgcaggaacc acctatgcag
      301 gggctacccg tttcttgaat gaattaaata atcctgttga cgaaattaca gaagaagtgc
      361 aggaagaagg agctgagcat caagtatttt atcagttggc ttccaatatt ggaacggtta
      421 cttatcattc tgcaaatact gatgctaacg gcaatcctat tgggcttcaa ttcactttaa
      481 caacgtctgc aaacaatgct acaagcggaa catttgttgt tactttaatt catttaccga
      541 ataaaacagc aaccggagtg gcagcaggag atatcaccaa tgccggtgga gctacggatg
      601 ctttggctat ttttccttta acaattctat aaattttgaa ataaaccgtg ttgaaaagcc
      661 aaactctttt taagcagttt ggtttttttt atttgatttt gtgagtttta tatattattt
      721 tttactaatt tagttatttt aatacctatt tactaaccaa aatacatttt ctatgaattc
      781 aagatttact tacttaatgc gtttgctatt aggtgttatc ctactggttt ttggcttaaa
      841 caaattttta aactttattc ccgcacctga attacctgaa aatgcggcta gttttattac
      901 ttctctagcg acaaccggtt atgtacttca ggtagttggt gtattggaaa ttcttattgg
      961 tcttttactt ctcactaaca aatgggtggc ttttgctctt gttgttttag ctccaatttc
     1021 agtcaatatc ttattatttc atttgtttct ggatatcccc ggtattagcg gagctttatt
     1081 agttgccatt attaatggaa ttttaatgta taaattgtgg ccaaaataca agccactttt
     1141 taactaattc gtatcatttg tttggttaca tttgtaaata tctatttatt tacaaatgaa
     1201 aaaaatcatt cctatgtttg caatagtttg tgttatttca gtaaatgcac aacctacctc
     1261 ttcttcgttt gcctatcctg aaacaaaaaa ggaagtggtg aaagacactt attttggcac
     1321 tgaagtgagt gacccttatc gttggttaga agacgaccgt tcagcagaaa caggtgcgtg
     1381 ggtaaaggcc caaaacgaag ttacttttgg ttatctttct caaattcctt ttcgaaatca
     1441 aatcaaagag cgattagaga aactgtggaa ttacgaacgt atttctgctc ctttcaaaga
     1501 aggggattac acttattttt accggaataa cggtttacaa aatcaatcgg ttatataccg
     1561 aaaagataaa tccgggaaag aagaactctt tttagacccc aatacatttg ccacagacgg
     1621 caccacctcc ttggctgata tatcgttttc taaagacggt tcgttgatgg cttactccat
     1681 ttcagaagga ggaagcgatt ggaacaaaat catcatcatg gatgccgttt caaaaaaagt
     1741 attagaatct cctattgttg atgtaaaatt cagtggaata gcttggttag gaaacgacgg
     1801 attcttttat tcgagctatg acaaaccgga aggcagtgaa ctttcggcca aaacggacca
     1861 acacaaatta tattaccaca aactgggaac aacacaaaaa gaagacaaaa tagtatttgg
     1921 tttgaatgaa aaaagaaggt atgtaagcgg agctatttct gaagatcaac attatttgat
     1981 tataacagca gctaattcta caaccggaaa cgaattatat attagagatt taaatgaccc
     2041 aaacagcaaa cttgaaacca tcgttgccaa ttttgacagt aataattata tcattgacaa
     2101 tataggaacc aaactgttta ttgtgaccaa tttgaatgct ccaaatcaaa aagttgtcac
     2161 tgtagatttt aaaaatccaa ctccggaaaa ctggaaaaat tgtattgcag aaacggatta
     2221 tgttttgtct ccctctaccg gagcaggata cctttttgct aattacatgg aagatgctat
     2281 ttcgcaagta aaacaatatg attatgacgg aaagttagtt cgtgaaatca gtttacccgg
     2341 cattggatct gccggcggat ttggaggtaa aaaaacagaa accgaattgt atttttcctt
     2401 ttcaaattat ttaactccgg gcacaattta ttcttttgac cccaaaagcg gaacttcaaa
     2461 ggtatatgaa aagcccaaag tagatttcaa ttccgacaat tatgaaagca aacaggtttt
     2521 ttatacttca aaagacggaa caaaaatccc aatgattatt aaccataaaa aaggactaaa
     2581 attagacggt aaaaatccaa ctatccttta tggctatggt ggttttaatg tgagtattac
     2641 tccaaatttc agtgttgcca atgctgtttg gatggaaaac ggagggattt atgcggtagc
     2701 aaatttaaga ggtggtggtg agtatggcaa aaaatggcat gatgccggaa cgcaacaaaa
     2761 aaagcaaact gtttttgacg attttattgc tgccggtgaa tacctgattg ctcaaaaata
     2821 tacttcttct gattatttag ccattcgggg tggttctaat ggaggcttat tagttggtgc
     2881 cgtcatgact caaagaccag acttgatgaa ggttgctttg ccagccgttg gtgttttgga
     2941 tatgctacgt tatcatacgt tcacggccgg agccggttgg gcttatgatt atggcacagc
     3001 caatgacagt aaagaaatgt ttacctattt aaaaaattat tcccctgttc acaatgtgaa
     3061 aaaaggagtg aaatatccgg ctactttagt aacaaccggt gatcacgacg atcgggttgt
     3121 accggcacac agttttaaat ttgctgccga gttacaagac aaacaagcgg gtgaaaatcc
     3181 tgtgttgata agaattgatg tcaatgcagg tcacggagct ggaaaaccca tttcaaaaac
     3241 aatagaagaa aatgccgatg ttcaggcatt tacattgtat agtatgggga taaaagaact
     3301 taaaaagtaa cgcaaattat caaaaaaatg cctttcatta gaaaggcatt ttttttaata
     3361 gtttaattct atgtaaccaa tatacctact tattcttgct tttcttctat tgatataggc
     3421 ggatgaatta ctggctttaa actttcgtgg attaggtaaa atagcggcta tcccggcagc
     3481 ttcctttcga gttaattttt ttgcgtcttt atggtaccaa accattgaag cttcttgggc
     3541 tccataaact ccatcaccca tttctatact attgagataa acttccatga ttcgttcttt
     3601 cccccaaatg agttcgatta gtaccgtaaa ataggcttct aatccttttc ggaaataact
     3661 tcttccttgc cataggaaaa catttttggc ggtttgttgc gaaatcgtgc tacctccttt
     3721 gatttttttc cctttctcat tgttttccat tgccttttcg atggctttaa aatcaaaacc
     3781 tttgtgcgtt aaaaaattag catcttcgct ggcaattacc gctttttgaa gattaggtga
     3841 aatctcttca attggaaccc aatcgtggct acatgtcatt ggtttaccgg ccattttatg
     3901 ctcaacagcc cttatagcca ttaaaggcgt gaagggaacc ggaacaaact tgtatagaat
     3961 aaccataaag atagaaattc caaaaaacca taaagctgct ttccaaagaa aacggagtgt
     4021 tttttttatc ataattactg ttcaattaaa tctgctaatt ctttcccaac aatacttcca
     4081 atcgctacac ccattccgcc caatcgaaca ccacaataaa catgttcaga aagttgtttc
     4141 acaataggtt tttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccaatc
     4261 gaacaccaca ataaacatgt tcagaaagtt gtttcacaat aggttttttt tgcttcccca
     4321 ctcccataat tccgctccaa cgctgttcga tttcaaaagc ttgattgggt aaaattacag
     4381 atgttaataa ttgttctaat ttgttttgga ttcgttccgt ttggtcaaaa cttgttgttg
     4441 tttctccttc aaaatcgagg tttcttccgc ctcctagtaa aattctgtct tcgaaatttc
     4501 taaaataata atatccttta tctaaatgaa acgttcccat aatttctaaa ttaggaattg
     4561 gttttgtaat gagtacttgt gcccgtgccg gttttacttc gccctttgtt atttcacccg
     4621 caaaaccatt cgtggcaaag aggagtttat tggtagagaa agaaaaatcg cccaaaacca
     4681 cttctactcc ttcccttttt tcaacataag aggttactgt ttgttgattt aaaattaaaa
     4741 catcctgttg aatggccaat ttcaggaggt attgtatcat gtttccggta tcaatctgtg
     4801 cttcataggg attgaacaaa acacagtctt gaattccttg aaaagcaaaa cgattaactt
     4861 cttttgaaaa aacttcattt ttaaataacg gtttcagtag ctcattgata aaaggaagtt
     4921 gttgcaaaca ttcatggtag gtagattctt cttcttttaa aaaaagttca taccctccaa
     4981 atggtttcaa atccagcact tcatctccaa ttcgtctccg aagtaattgt aatccattcc
     5041 aacgtttttg aaccagttgc aagacttctt cttcagtatg tgaacgcaaa tcatctacta
     5101 tttcggaaac agacccaaaa caggcaaaac ctgcattttt agtgcttgct ccttgtggta
     5161 aaacaccttt ttcaaggatt aaaatcttag ccgagggata tttatcacgc aactgtaagg
     5221 cacaatgcaa gcccacaatt ccactgccca caatggtgta gtcaactttt gtaaaccaat
     5281 ttttaagctc ccaaaaacta aattgcattc tcaataaatt tttataaatg tactttttat
     5341 tttaaaaata gaaaaaaata acgctatctc ctgaaaaata aattatgact taccatcatt
     5401 ttaactttaa ccttaccatt ttgttatatt ttttatggtt taggacacaa attgttaata
     5461 aatatgtttt taacccgatt atctgcatga aaaatatttt acatttgatt ttattaacta
     5521 attaaccata ttttatgaag cttaaattac ttacgattgc tctggtagca agtgtgagtt
     5581 tttcttttgc tcaagagaaa aactcttttt ggaaagcctc tactttaaaa agtacttctg
     5641 aacttttaga aagtaaaatg caacttccgc aaaacaacgt atttgaatta gatgtaatcg
     5701 ggataaaaaa tgctctttct acatcacctc aacgatttgc aaactctaaa ggaggaacga
     5761 ttatttcctt cccaaatgca gaaggtgcaa tggaacgttt cagtgtttat gaaaattcta
     5821 tcatggcacc tgaattagca gcgaattatc ctgaaattaa atcgtatatc ggaattggaa
     5881 ttgacaatcc tacttctaca ttatacttca gtatttcacc acttggtttt aaatcgatgc
     5941 aattaaatgc cggaaaatca gccgttttta ttgagcctct ttctgcagat ttaacaactt
     6001 attctgtcta taaaaaagcg gataaagtga aaaacttttc tccttttgaa tgtgctgttt
     6061 tagaccaagt tcaaaatcaa attgaccctt ctgcattacg cccaaatgct gatgatagcc
     6121 tattgagaac cttccgctta gcaatgtcgg taaccggtga atatacagct tactttggtg
     6181 gcacaaaagc tttagcttta gcagctatta acaacactat gacccgtgta aatggtgttt
     6241 ttgaaaaaga ttttgcagtc cgtatggtct taattgctaa tactgatttg gttatttata
     6301 ccaatgcttc taccgatcct tattctgctt cttcaagtat gtcgaattgg aattcacaat
     6361 tacaatcaac tttaacttcc gttattgggg aagctaatta tgatgtaggt catttatttg
     6421 gtgcaaccgg aggcggtgga aatgcaggct gtatcggatg tgtttgtgtt aatggttcaa
     6481 aaggcagtgg ttatacttct ccggctgacg gaattccatc gggagataat ttcgatatcg
     6541 attatgttgc ccatgaatta ggtcatcaat ttggtgccaa tcacactttc acgttcagca
     6601 atgaatcagg aaccggagct caaatggaac cgggaagtgg ttctactatt atgggttatg
     6661 ccggtattac tacgaaagat gttcaacctc attccgatgc ctattttcat gctatcagta
     6721 tccaacaagt tactaataat gtaaaagcta aaacctgtca gacgaatact tccactggaa
     6781 atgctgttcc tactgcgaat gcaggattag attatacagt gccaaaaagt actcctttca
     6841 tgttaacagg aaccggaact gacgcaaacg gagatgtatt gacctataat tgggaacaaa
     6901 tggatgccgg aacctccact actactacac caagtgctac aaaaaccgct ggtccaaatt
     6961 tcagatcata caattcgtca acttctccaa caaggtattt ccctagaatg caatctgttt
     7021 taaccggagc aacaactact acagggacag aaattttagt agaagcttta ccttcagttg
     7081 ccagaacatt taatttcaga ttaactgttc gtgacaaccg agccggaggt tcaggaaaca
     7141 atagtgatga tatggttgta actgttaatg ctactgccgg accttttact gttaattctc
     7201 caaatacagc tgtttcctat gcaggaggaa gtacacaaac cattacatgg gccgttgccg
     7261 gaactactgc taatggtgtg aattgtgcca atgttgacat cttattatcc acagatggtg
     7321 gaaataactg gaatactttg cttgcagcca ctccgaatga tggtacagaa gcagtgacca
     7381 ttccaaatac tgccggaaca caaaatagaa taatggtaaa aggtaccaat catattttct
     7441 ttgatgtttc caatacaaac tttacaatta cagcaggctc ttcagatact gttgctccaa
     7501 ctgctcctac tttagttgca tctgggacaa cacaaactac taccaattta tcttggtcag
     7561 gagcaactga taacgttgct gtaaccggtt atgatattta cagaggaaca actttacttg
     7621 gatcaacagc ctctacgact tatgccgttt caggattaac tgcttcaaca acttattcct
     7681 ttacggttaa agcaaaagat gctgccggaa atgtttctgt tgccagtaat gctgttagtg
     7741 tgacaacact tgcaccagtt gcagacacaa ccgctccaac cgctccaact ttgtcagcat
     7801 ctggaacaac acaaactacc accaatttat cttggtcggg agcaactgat aacgttgccg
     7861 taaccggtta tgatgtttat agaggcacaa ccttacttgg ttcaaccgct tctacaaatt
     7921 atgccgtttc aggattgact gcttcaacaa cttattcctt tactgttgta gccaaagatg
     7981 ctgccggaaa tgtttctgtt gccagtaatg ccgttaatgt aactacatta gctgtctcaa
     8041 ctgttacgta ttgtgcttct caaggaaata gtactgcaga tgaatacatt aaccgtattc
     8101 agttgggaac tatcaataat ttatcaggaa acaacagtgg ttacggaaac tttactgcac
     8161 tatctactaa tttagtgaga ggaactagta attctatcac cattacaccc gcttggacag
     8221 gaactgttta tagtgaaggt taccgcgttt ggatagacta taaccgagat ggtgattttg
     8281 ctgatagtgg ggaacaagtt gtaaacgtta ctaaaactaa aacaactcca ataaccagaa
     8341 gttttactgt acctacaaca gcgttaattg gctcaaccag aatgcgtgtt tctatgaaat
     8401 ataacgcttc tccaacttat tgtgaaacat tctcttacgg tgaagttgaa gattatacgg
     8461 taaacatagt tactacagcc agaggagaac aggaaattgt tttagaacct gtttctaaag
     8521 agtcatttac cttatatcca aatccaatta aaggagattt agttcatatt gatgctgaaa
     8581 cgcaatttac aacttataaa atcttgaatt tattgggtca agtgattgct cagggaaaaa
     8641 ttgaaaacaa aacagtttta gtttcaaatc ttgcttccgg aacttattta ttagaagtgt
     8701 cagacaatga aacttctgct gtgaagaggt ttatcaaaca ataagtaatt catttataaa
     8761 tctaagccat ctcgttgagg tggctttttt tatggaaaaa aatagttact tttatacttt
     8821 gaaaatcaaa tacaatgaaa aaaataattt taatcctcgc ttttataatg ggagcatcgt
     8881 acgtttcagc acaacaaatt ttaactcctg aattactttg gaaacttgga agagtttctg
     8941 ctttaggcat ttctaaagat gggaaaaatg tgatttacaa agtttcttta ccttcggtag
     9001 aggaaaataa atctgattct aaattttaca gcatttctgt gaatggtggt tttgcaacag
     9061 aaattcctga aactaaagaa cttttgactg ataaaaacct ttcccccgac ggaaaatatt
     9121 tgctttccaa caaagaagtt aaacttgaaa aaatattggg taaagacctc tatcctgaac
     9181 ttgaaaaatc aaatgttcaa gtctataacg gattagatta tcgccattgg gatacttgga
     9241 atgatggaaa tcataatcat gttttttatg ccgatgcaaa tgggaatgac aaatctgttg
     9301 ccattgacat catgaaagga gagccttatg attctcccca aaaaccattt ggtggggacg
     9361 aagatttcac ttggtcgcct gatggaaaaa gtatcattta tgtttgtaaa aagaaatctg
     9421 gaactgccta tgccacatca acaaatactg atttatatga atataattta gaaaacaaaa
     9481 caaccaaaaa tctaactgaa tctaatttag gatacgatac aaatcccctc ttttctccta
     9541 acggtgattt gacctggttg caaatgaaac gtgatggtta tgaagctgat aaaaatgata
     9601 tcattgttcg atttaaagga atggacatga acttaactgc caattgggac ggaacagtag
     9661 atggtttcaa atggagttct gatagtaaaa aaatctattt caccgccgct gtggatggta
     9721 caaaacaatt atttgaagtt aactttcccg gtttaactcg aaaagctgta atggttcgtc
     9781 aaattacaac cgggactttt gatgtgaatg atttggttgg attcaccgga gataaaatta
     9841 ttgtaacccg aacagacatg aatcattcta aagaaattta ttcctatgat ttaaagaaaa
     9901 atgcttggtt aaaattaact gaagtaaatg acaaagccta tgctaaattg tctttaccta
     9961 cttatgaaaa gcgatatgta accacaacag acggtaaaaa aatgttggtt tgggttattc
    10021 ttcctcctaa ttttgataaa acaaaaaaat accccaccct tttatattgc caaggtgggc
    10081 cacaaagtgc tttaacacaa agttattcct tccgttggaa tttttcttta atggcttcgc
    10141 agggttatgt tattgtagca ccaaatcgaa gaggaatgcc aggacatggt gtcgcatgga
    10201 atgaacaaat cagtaaagat tggggtggtc aagtgatgga tgattattta tctgccatcg
    10261 atgatgtttc caaagagtcc tatgttgaca aatcaagact tggtgctata ggtgcgagtt
    10321 atggtggtta ttctgttttc tatttggccg gaattcacaa taaacgcttt aaaacattta
    10381 tttcgcattg tggtgttttt aatttacaaa gtatgtacgg caccactgaa gaggtgtttt
    10441 ttacagattg ggatagtgga ggcccttatt gggaaaaaga taatgctgtt gctcaaaaaa
    10501 cctatacgca attcaatccg attaatttag tttcaaaatg ggacactcct attttaatca
    10561 ttcagggagg caaagattat cgtgtgccca ttggacaagg acaagaagct tttcaggcag
    10621 ctcaattacg tggaataaaa agccgattta tgttgttccc tgaagaaaac cattgggtac
    10681 ttaaaccaca aaatgctttg gtttggcaac gagaattttt tggttggttg aaagaaacat
    10741 tgtagaaaaa gaaataatac taaaataaaa atcccaagct ctcacttggg attttcttat
    10801 ttattccgga tcattcattt taaaactttc cataaatttt gtggtatagt ttccggcaac
    10861 ataatccggt tcatccatta attggcggtg aaaaggaata gttgttttaa ttccttcgat
    10921 ataaaactca tctaaggccc gtttcatttt gctgattgct tcttctctgg tttgagctgt
    10981 agttattaat ttagcaatca ttgaatcata attaggcgga atgctatatc ctgcataaac
    11041 gtgtgtatct aaacgaactc cgtgtcctcc cggtgcatgt agtacagtaa ttttacccgg
    11101 tgaaggtcta aaatcattat atggatcttc agcattaata cgacattcga ttgaatgcaa
    11161 ttgtggcaaa taatttttac ctgaaattgg cacaccggca gccactaata tttgttctcg
    11221 aatcaaatcg taatcaatta cctgttccgt aataggatgt tccacttgaa tacgggtatt
    11281 catttccatg aaatagaagt ttctgttttt atcaaccaaa aactcaactg ttccggcacc
    11341 ttcatattta atgaattcag ccgctctaac ggcagcttca cccatttttt cacgcaattc
    11401 gtccgtcata aatggtgaag gagtttcttc tgttaatttt tgatgacgac gttgtactga
    11461 acaatctctt tccgaaaggt gacatgcttt tccataagca tcaccaacaa cctgaatttc
    11521 tatatgacgt ggctcttcaa ttaatttctc catatacatt ccgtcattac cgaatgcagc
    11581 agctgattcc tgacgagcac tttcccatgc tttttgaagt tcatctgctt tccaaactgc
    11641 acgcattccc tttccgccac ctccggcagt tgctttaagc atgacaggat aacccatgtc
    11701 tttagctatt tgagccgctt gttcaaaaga ctccaacaaa ccttcagaac caggtacgca
    11761 aggaactccg gctgctttca tggttgcttt tgctgaagct ttgtctccca ttctgtcaat
    11821 catttccgga gaagctccaa taaattttat gccatgttct tggcaaattt tagaaaactt
    11881 agcattttca gataagaaac cataaccggg gtgaatagca tcagcatttg taatttcagc
    11941 cgccgctata atatttgaca tttttaagta agacaaatta cttggcggtg gaccgataca
    12001 aaccgcttca tcagcaaact taacatgaag gctttctgca tctgctgtag aataaaccgc
    12061 tacggttttg atacccattt ctttacaggt tctaattaca cgcagtgcaa tttctcctcg
    12121 attggcaatt aatatttttt taaacataga ttttaatttg aaaattaaac aatttagctt
    12181 cgctcagttc agctaagcct ctagtgaaaa tttgaaaatg aattcaaatg attacatttt
    12241 ttcaaattaa gacggatcta ctaaaaataa aggttggtca aactcaactg gtgacatatc
    12301 atcaactaaa attttgatga tttttcctga aacttctgat tcaatttcgt taaataattt
    12361 catagcttca attacacaaa cgggatctcc tttagaaatt gtagcaccta cttccacgaa
    12421 tggagctttg tctggagccg gttttctgta taaagttcca atcattggcg atttaatcgt
    12481 aatgtatttt gaattatcat caacagccgg agcagctacc ggagtaacgg gtgcaaccgg
    12541 ttgaggcatt tgatgttgtg gcatcatctg ttgcattggt tgtgcagcag gataatgttg
    12601 gaaataagaa gcttcagcag caccggcttc agtagttttg atggtgattt taaaatcatc
    12661 catttctaat tttacctctg tagctcctga tttggctaca aacttgatta ggttttgaat
    12721 ttctctaata tccatgatgt ttttgattta ttttagtttt gtttattttt tatcgtaagc
    12781 ccatttcaga tagattgcac cccaagtgaa tcctcctcca aaagctgcaa aaattaaatt
    12841 atctcctttt tttaatttag tttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnntgcatt agacattcca taaagaaaac tggaacaagc ggcttgtaaa tcataggcaa
    13021 aagcattcac tgctccaatt tgactggcta cataaacccc tgtggaagcc actggcatat
    13081 ccggtgtagc tgtagccata attactaaat caatttcttc cggattgatg ttatttttct
    13141 caaatagatt ttgagcagct tttatagcaa gaaaagaagt tcctttacct tcttctttta
    13201 ataatcgtct ttctttaatt cctgttctgg aagttatcca ttcgtcatta gtatcaacca
    13261 atgtttccaa tactttattt gacaacacaa aatcaggaac ataagcaccc actgcggtta
    13321 tggcggctgt tattttactc atagggtttg ctagtttttc ttttcccata caggttaaaa
    13381 agaggtggaa attacaaaaa aaaatctaaa ctcaatcgat ttctaatcgc ttatttcttt
    13441 aaaatatagg tataacaaaa aaactctcaa caagtgagag tttattttta aactataaaa
    13501 aaagtactaa gcaacagctt cggttttgtc aataacaact tgacctctgt agtacatttt
    13561 tccttcatgc cagtaagctc tgtggtataa atgtgcctca cctgttatcg ggcaagttgc
    13621 aatttgagca acagttgctt tataatgagt tcttcttttg tctcttctgg tttttgaggt
    13681 ctttctcttt ggatgtgcca ttttactata ttatttatcc gttaatagtt tttttaattg
    13741 atcccaacgg gggtctgtct cttctgtttt cttttctact tttttttctt tgactgtcaa
    13801 ttcttgcagt ttatcaattg cttctgattg taatgttccg tcttttactc cgggatgaac
    13861 tctttttaaa ggaattgaca atacaatcat ttcataaatg tattgtgaaa tatcgacttg
    13921 atgttcccca tgaggtaaaa tcaacaattc ttcattttca tcattgaaag catctccaaa
    13981 ctgaacaatt aatttgattt tgcctttaat tggtaaatca aacggttcac ttgtcaaatc
    14041 acaaggaaca tttacggttc ctttgtgttt aaaatgtaac tctaacatgg ttgttttttt
    14101 ttctaaaaca accgttgttt tgatatcaca actttcaaaa tcatcatatt cgaagcgatc
    14161 aaagaacgtt ttatctattt gatagtcaaa ttgatgtttt cctagcttta atccgataaa
    14221 cggaattaaa aattcattag atactttcat ctccacaaca atttgtccca aaaatttggt
    14281 ttgcaaagat ataaaattat cgcaaatcca atacggttat aaacattttt ttgtttaaaa
    14341 ctgtttttct tttgttttaa gcggtttttc agttatttga gcatactcca ttctggattt
    14401 ataaatgtcc aatgccaaat aaactgcctc tttaaacgaa ttgaaatcag cttgattttt
    14461 tccggcgatt tcaaatgcag ttccgtgatc cggagatgtt cgtattttat ttaatcctgc
    14521 tgtataattc actccgtttc caaaagataa tgttttaaag gggattaacc cttggtcgtg
    14581 gtaacatgca atgactgcat caaatttttc ctgtgaattg tttccaaaaa aactatctgc
    14641 tgcatagggc ccaaaaacaa atgtgccttc atcaaataac ttctttatag ttggtctgat
    14701 gacttcatca tcttctttcc caattatacc atggtcacca caatgaggat taagtcctag
    14761 aacagcaatt tttggtttgc tgattttaaa atcctgcatt aatgattgtt taactgttgc
    14821 gattttttgt ctgattaatt tttctgttaa atattttgaa acttccgtga caggaatatg
    14881 gtcagtaaac aaaccaaccc gcaaagagtc attaaccatc aacatcaacg catcaccttc
    14941 taattcctga ttaagataat cggtatgccc cggaaattta aattcttctg attggatatt
    15001 atatttattg attggagctg ttactaaaac atctatttct ccttctttta aagctttagt
    15061 cgcagctaca aaagatttta tcgcataact cccaacaata tcatccgcta cgccgtattc
    15121 taaattaaaa ctgtctctcc ataaattaag cacatttatt ttcccaagta caatttgatc
    15181 taacttgtca attccgtgca gtaaagcagt tgattcaaaa ctttttctaa gaaaagacaa
    15241 catcctaaca ttagcaaaaa taacgggagt acaaagttcc aacattcttg gatcttcgaa
    15301 ggttttgagc acaacttcgc ttccaattcc gtttaaatct ccaattgaaa ttcctaccag
    15361 aatattttct gcttttttca ccatggcttc aagatattta ttgctacttt tgaagtgcaa
    15421 atttaagaaa ataaaacgca aatgtttaca ggaattatag aaactcttgg aatcatcaaa
    15481 gatattcaaa gagaaggcaa caatcttcat gtatcagtat cttcttcgat aacttcagaa
    15541 cttaaaattg accaaagttt agctcataat ggaatttgtt taactgttgt tgccataaaa
    15601 aatgacgttt ataaagttac agccattaaa gaaaccattg ataaaacaac cattggtaat
    15661 tggaaaatag gtgatttaat caatttggaa agagcaatga aactggatgc ccgattagat
    15721 ggacaccttg ttcaaggaca tgttgatcaa attggtgttt gcaatgccgt taaagaatct
    15781 aacgggagtt ggtattttac ttttgaatat gacagcaaac caaacaacat cactattgaa
    15841 aaaggctcta tcacggtgaa tggtgtaagc ttaacagtgg ttaattccaa gcaaaaagaa
    15901 tttagtgttg ctgtaattcc ctatactttt gaacatacta actttcagac ttttaaagtt
    15961 ggcactaaaa taaacttaga atttgatgta attggtaaat atgttgctca attatatgag
    16021 tataaaaaat aattagtttt tcatatcaaa aaaaaactcc gaattacttc ggagtttttt
    16081 ttatcgtttg tttattgttg attttgtaaa aagcaaatac tacaccaaca actaacagca
    16141 ggtaaatcca attatcgatt ggtgtaccgg gcggaggcgg agttggtgga ggcggattat
    16201 ttgagccttg agctaatgca gttgcactag atagtatcaa aataaaacca cccaatatat
    16261 ttataaacca gtttgggaca attctcatag ggtgtaaaag taaataaatt tattctttaa
    16321 caaaatattt aggcaaataa agtaaaaatc tctttaaaac aaaaactaag ttgttatttt
    16381 tttctatact tccttttaaa atcataattc atcggcaaaa gtacatcttt tttgacaact
    16441 tcctatattt caaaattata ttaacgtaaa atattcaatt ttggtttatc tttcactaaa
    16501 taatttattg aaataaaaaa aaccttccta tttggaaggt ttcttgtggt cccacttggg
    16561 ctcgaaccaa ggaccacctg attatgagtc aggtgctcta accaactgag ctataggacc
    16621 ggtttagaga gtgcaatatt actactattt ttgatttact caaaatataa attgtattta
    16681 ttgattcctt ttagccaact ctttttaatt atgactcaat tatctgattt agaattactc
    16741 aaaaagtcat tacttttgct gaaaattaca gaatgcgaaa aatatactac ttaaagacct
    16801 gtgatacctg taaaagaatc atccaatcat taccaaaaac ggacggcttt ttgtttcagg
    16861 acatcaaaga ggaacctata accgtgaaac aattggaaga aattcatgtt ttagccggaa
    16921 gttacgaaat cctttttagc aaaagagcca aactatataa agaaatgggc ttgaaagatg
    16981 aaaatctttc cgaatccgat tttaaacgat acattttaga gcattatact tttttaaatc
    17041 gtcctgtaat tgttattgat aaaactattt ttattggtaa cagtcctaaa acggttcaag
    17101 ccgccattga ttttttgaca aatgaataaa agaaattggg ctttagtggc cgccatgttg
    17161 gtttctataa tttacggaat cacttttacg attgctaaag atgtaatgcc aaaatatgtc
    17221 gaaccatttg gatttattct ccttagggtt ggtggttctg tttttctttt ttggctagtt
    17281 tcattttttg gcccgaaaga aaaaattgct ttagtagatt ttcctcgaat tgttgcggct
    17341 gcattttttg gtgttgcttt taatatgctt acttttttta aagggttaag ttatacatca
    17401 cctataatgg gagctgtttt aatggttacc acacccatga tagttttaat tctttcagcc
    17461 ataatcatga aagaaagaat gaaaaaacgt aaaattttcg gaatagtttt aggtttggca
    17521 ggaacgatat cactcatcct ttacggaaaa tcgctaatta atgctccaaa tgaaatgcta
    17581 ggaaatttgt tggtttttgt gaatgccgtt tcttatggtt tttatctcat tatcgttaaa
    17641 aaattaatgg ataaatacaa tgcgtttacg tttgttaagt ggatttatac atttggcttt
    17701 ttaatggtta taccttttgg atggggtgaa tttaacgccg taaattgggc aattattcca
    17761 acggatattt attggaaaat tggatttgtg gttgtgtttt cgaccttttt aacctatttg
    17821 ttaaacttag tttcgatgcg tgaattaaaa cccacaaccg ttgctgtttt tatctacctt
    17881 caacccttat ttgcaactgt ttttgccatc agtctaggaa aagatgacct aagttggatc
    17941 aaaatcggtt cggcaatcct aatttttaca ggggtttatt tggttacaca aaaaaagagt
    18001 actcaatagt caagtattag tttccaattc tttgaagaac tgactacttt ccctctaaac
    18061 actgaacact tttccttatc tttgaaaaca ttttttataa tttatgatac aatcgatgac
    18121 cggttttgga aaagcatctt tgcaacttcc aaccaaaaaa attacagtag aagtaaaatc
    18181 actaaacagc aagggtttag atttgaatgt cagaatgcct tctgtttatc gtgaaatgga
    18241 attaggtttg cgtaatacca ttgctcaaaa attagaacga ggcaaagttg atttctccat
    18301 ttatatagaa gtaaccggcg aagaaacatc ttctaaaatc aatgtgccaa tagtgaaagg
    18361 ttatatcaat caaatgaaag ccgttattcc aaatgcagac gaaacggaat tgatgaaaat
    18421 ggctgtcaga atgccggatg cgttaaaaac tgaacgtgac gaaattgatg aaaatgaatg
    18481 gaaagagatt atgaaagtgg tagatgatgc gatgaaaaat attgcctctt ttcgtttaga
    18541 tgaaggaaaa tcattggaaa atgaatttgt aaacagaatc gaaaacattc gtaattatat
    18601 gaatcaagcc gtgagtatgg atactgaacg aattgaaact gtgaaaaacc gtttacgaac
    18661 tgctttggac gaattggaag cgaatgtaga tgaaaaccgt tttgagcaag aattgatttt
    18721 ttatcttgaa aaatatgaca ttactgaaga acgcgttcgt ttagaaaatc atttgaatta
    18781 ttttatcgaa accatagccg gaacagaagc caatggaaga aagttaggtt ttattaccca
    18841 agaaataggt cgtgaaatca atactatggg atctaaatcc aatcattcag aaatgcaaaa
    18901 attggtcgtg atgatgaaag atgaattgga gaaaattaaa gaacaagttt taaatgtttt
    18961 ataattagaa aataaaggaa agaataaaga ggaaagaaaa taacactatg aataaaggaa
    19021 aactattagt cttttctgcc ccatccggtt cgggaaaaac caccattgta aggcatttgc
    19081 tggctcaatc cgatttgaat ttggaattct cggtttcctg tacaacccgt gaacccagag
    19141 gagaagaagt gcatggaaaa gattattatt ttatatcact aaaagatttt aaaacccaca
    19201 tcaaagccga agagtttgtg gaatgggaag aagtgtatcg cgataatttt tatggcacac
    19261 ttaaaagcga agtcgaacgc atttgggcaa aaggtaaaaa tgttattttt gatatagatg
    19321 tggctggcgg attgcgaatt aaatcaaaat tcaaacaaga aaccttggct gtttttgtaa
    19381 agccacccag tattgacgaa ttaaaaatcc gtctaaaaaa acgttctaca gaatctgagg
    19441 ataaaatcaa catgcgaatc gcaaaagcat ctgtggaatt agctactgct ccacaattcg
    19501 acaaaatcat taaaaactat gatttggagg tggcaaaagc agaggcatat gaattggtta
    19561 aagaatttat ttgtcaaaaa aatccaaatg attaaataaa atcaaaatga aaatcggatt
    19621 atatttcgga acgttcaatc ccattcatgt ggggcattta atcattgcca atcacatggc
    19681 ggaatattcc gatttagatc aagtttggat ggtcgtaaca ccacataatc cacacaaaca
    19741 aaaaagtacg ttgttagata attatcatcg gttggaaatg gtgcatttag ccaccaagga
    19801 ttatccgaaa attaaacctt ccgatattga attcaaatta ccacaaccca attatacggt
    19861 aaatacgttg gctcatttgc aagagaaata tccaacttat gagtttgcct tgattatggg
    19921 tgaagacaat ctgaattcgc ttcacaaatg gaaaaattat gaagtaattc ttcaaaatca
    19981 tgacattttt gtttatccga gattaaattc cggtttgatt gacgaacaat ttatcaatca
    20041 tccaaaaatt catcgcgtgg gtgctccggt gattgaacta tcctctactt tcattcgtga
    20101 aagcatcaaa cacggcaaaa atgttagagc cgttttaccg gaacatgtat gggctttttt
    20161 agatcataat ttgttttata gaaaataaag tacctcttaa cactccttta ccacttgttc
    20221 catcaatcgt tttgtaactt tg
//
LOCUS       DAOTCW010000031        20052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1456_length_20052_cov_44.233785, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000031 DAOTCW010000000
VERSION     DAOTCW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 20052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20052
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1456_length_20052_cov_44.233785"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..74)
                     /locus_tag="P5335_04165"
     tRNA            complement(2..74)
                     /locus_tag="P5335_04165"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Met,seq:cat)
     gene            complement(170..1147)
                     /locus_tag="P5335_04170"
     CDS             complement(170..1147)
                     /locus_tag="P5335_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRZ74107.1"
                     /translation="MEKSTNQKIQLGIFVILGLAIFVAAIYFIGNKQNMFGKTTHLTA
                     VFNNVNGLQVGNSVRYSGINVGTVREITMLNDTMIRVTMVIEDKIIPHIKKNAIATIT
                     SDGLVGNMIINIIPGKGEGLSVQSGDEINTYSRIRTDDILETLNVTNENAALLTADLL
                     KITKEITQGKGTVGLLINDTLMAKDLKETMHYLKITGKGTAASVQNLNKLIASLEDRN
                     NVVGVLKDTAVASNIKEIVLSLDRSSKEMEKVITNLNETITNIKEGKGALNYLSNDPK
                     LVKQIDSTMTNLNQASLKLNEDLEAMKHNFLLRGYFKKLEKEKAKANKK"
     gene            complement(1156..1908)
                     /locus_tag="P5335_04175"
     CDS             complement(1156..1908)
                     /locus_tag="P5335_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRZ74108.1"
                     /translation="MEEIREYRKPVVEITNLYKSYGSNKVLDAFNLKLYEGENLVVMG
                     KSGSGKSVMIKCLIGLEEPDSGSISIMGQAIKTLSREELDELRTEIGFLFQGSALYDS
                     MSVRENLEFPLRRHTKKFGVIKDTTPLVMEALQNVGLAHAIDLMPNELSGGMKRRVAL
                     ARTLILQPKIILYDEPTTGLDPITAKEIILLMKNIQQQYKTSSIIITHDVDCARVISN
                     RMILLVDGTNYAEGTFDELKALKDEKVQAFFK"
     gene            complement(1911..2693)
                     /locus_tag="P5335_04180"
     CDS             complement(1911..2693)
                     /locus_tag="P5335_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRZ74109.1"
                     /translation="MKVIDKILQTSKTFLEETGELAYFSARFFKEATKRPFEFKEFLR
                     QCYNMGNSSLLLVGVTGFIIGLVFTLQSRPTLQEFGAVSWIPSMVGISIIREIGPIIT
                     ALICAGRIGSGIGAELGSMRVTEQIDAMEVSGTNPFKYLVVTRIMATTLMLPILVFFG
                     DAIAIYGSFLVENIKGDVSFLLYFNQVFKHLEFGDIIPATLKSFFFGFAIGLVGCFKG
                     YTCKKGTAGVGLAANSAVVYTSMLLFIIDFIAVFLTDIFYDL"
     gene            complement(2822..4024)
                     /locus_tag="P5335_04185"
     CDS             complement(2822..4024)
                     /locus_tag="P5335_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74110.1"
                     /translation="MKAFISKCIFLFLALFYLSGFTQSDSLVVKQRDLKDVYYKVFKI
                     EDSLVSERDYQDKKAVFSLLPAPSSSSTGAERALVISFVTTFYLSELENTNMSTISFT
                     PSFTFSNQYIFPVQTYLYTKGNKYILTGDYRYMIYPQSTYGIGANNYENEQSVLDYQQ
                     WRLYQYVARRVKKQLYLGLGFLLDNYHNISEESFIEGETDYVKYMKNDFSDELAIGFA
                     FQTIYDSRDNITNPSKGMFMEFDYKFNSKFNDNMTDYSSVYFDLRNYRSFSKSKHRVW
                     ANKLFWWNTFGGNAHYLDLPSIGWDHYQKTGRGFTRNRFRSNALIYLESEYRTDITKD
                     GLLGAVFFANVSSVSAIDTYQFKKFNPAIGTGLRIKWSKINNSNISLDLGVSKQDVTF
                     RVGFYENF"
     gene            complement(4145..5143)
                     /locus_tag="P5335_04190"
     CDS             complement(4145..5143)
                     /locus_tag="P5335_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRZ74111.1"
                     /translation="MKLISVENHSGVPKYKQIIASVEAAIAEKRLQKGDKLPSVNKVS
                     LEFSISRDTVLLAYEELKKRGIVFAILGKGYYIKSVEFSFEQRFFVLFDELNAFKEDL
                     YTSILESIDNKAQIDIFFHHFNLEMFRKLINESNGNYSKYIIMPTNLVGAANLIKTLP
                     KNDVYILDQTNEELREYPSVHQNFVKDMYQALVKAKEKLDKYQQLILIFPGFKEPLGM
                     VEGFLKFCKEFNFGHEVIVDFEVRTIAVGNVYIIPNDRHLVQVIEQSKKQQLRIGQDF
                     GIISYNDTPLKKVVENGITTISTDFKAMGARLTEMIFTNKKEQMENQSQLIIRASL"
     assembly_gap    5421..5520
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5521..6135
                     /locus_tag="P5335_04195"
     CDS             <5521..6135
                     /locus_tag="P5335_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HRZ74112.1"
                     /translation="LEYISQHLEENYPGILMAEVTYMLTESNQVIVEFHAHSTEDTLL
                     NLTQHSYFNLDGHQGNVTNQDLIIHSESVLETNQENIPSGVLIKLSDNSFDFSNAKKA
                     PKSIDNTFTLQANQKVAAVLTSKLHNLKMTVLTDQPAVHVYVGGNCFGQIKGKEAADY
                     HPESGICFETQNYPDAPNHKNFPSAVLKKGERYHHQTIFAFETL"
     gene            6146..7234
                     /locus_tag="P5335_04200"
     CDS             6146..7234
                     /locus_tag="P5335_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HRZ74113.1"
                     /translation="MKNVLTLFTVLLTTFFLGSCSEDDSKTPIEPVVEDNFIRAADMS
                     YLPLIESENTVYYNADNQPEIALTTLKNAGCNTIRIRLWKNPADAHSGFNEVKAFAQR
                     VKQAGMKVWLTVHYSDTWADPGNQTTPVAWQNLSFADLKTAASDYTTTILTEIEPDIF
                     QIGNEINSGLLWPKGHLINNESQCLELLSTISSRIRSQSPTTKIMLHYAGIGSGATWF
                     YNKMTSVDYDYIGLSFYPVWHGKSLPDLKSTINSLGQTYNKKVVIAETAYPFTLTWND
                     WTNNLVGLENQLIPAYPATPEGQKNYLLAIKSLVKETTSGIGFAYWGTEWVAFRGNES
                     TNGSPFENQALWDFTNKAVPAMAAFKKD"
     gene            7237..8247
                     /locus_tag="P5335_04205"
     CDS             7237..8247
                     /locus_tag="P5335_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HRZ74114.1"
                     /translation="MKKTFYFLPLFLILFHSTLNFGQAFSKGADVSWLPQMEATGYQF
                     YDVDGSKKDCLQLLKDRGMNTVRLRVFVNPNDDKASGHCSKDETVAFALRAQKMGFRI
                     MIDFHYSDSWADPAKQNKPAAWAKLPFDQLAQAVYNHTFEVMTALKKAKVKTEWVQVG
                     NEIPGGMLWPDGSTNNWKQLGILLNKGYDAVKAVDKSIKVIVHLDEGDNNAKFRTFFD
                     NATAQNVKYDVIGLSYYPFWVKKDYTETIANLAFNLNDMVTRYNKEVMVVEVGGEYDK
                     VQNTKELLEATIKAVKNVPNNKGLGVLYWEPQGEKSWSHYSLSAWQADGKPSPALDAF
                     KE"
     gene            8252..10681
                     /gene="galA"
                     /locus_tag="P5335_04210"
     CDS             8252..10681
                     /gene="galA"
                     /locus_tag="P5335_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015782228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalA"
                     /protein_id="HRZ74115.1"
                     /translation="MLKNLFQPFGKLLLLYSFVLFSFVGKAQEKISLDDDWKFHFGHA
                     ANPEKDFDYSKTALLHKSNVFATTIVNPKFVDTAWTKVNVPHDWVVELPFVKSEQVEM
                     DSHGYKPVGGAFPETSIGWYRKHFTVDKAKSNKRFEIQFDGIYRNAEIWLNGFYVGTN
                     FSGYVGNSYDVSDYVNFEGDNVLVVRVDATQYEGWFYEGAGIYRHVWLNITDKIFIPQ
                     DGVFVHSEIKGKNAIVTIETTVQNNHLKTTGGLVYAYITDRNGKVLAKTAEQKITLGI
                     HKDITVKQKLNLNNVRLWSLEDPYLYKVISVVKSDNQIVHQTKTRFGIKTVKFDAKEG
                     FFLNGNHLKIQGTNNHQDHAGIGSALPDYMQYYRIKLLKEMGSNAYRASHNAPTPELL
                     EACDSLGMLVVDEQRLLNSSPEYIDQFKRILKRDRNHASVFLWSIGNEEQNIQGNDYG
                     KKIAQTLLAIQEDLDPTRTSTYAADMANDFKGVNEVIPIRGFNYREYAVADYHRDHPH
                     QPLLGTEMGSTVTTRGIYEKDEIRAYVPDQDITHPWWASKAETWWKLAAENKYWLGGF
                     VWTGFDYRGEPTPYKWPNISSHFGILDVCGFPKNIYYYYQSWWSTKDVLHISPHWNWP
                     EKVGKPIDVWVNSNADDVELFLNGKSLGKKTMPRNSHLQWDVTYEPGILEAVGYKKGK
                     KITTKVETTGNAYAVVLSPDKTILTADGKDATVFNISITDEKGREVPYADNMVKFYIS
                     GDAKIIGVGNGDPSSHEPDKCREGAWQRSAFNGKCQVIIQAGKTVGTVNFEAKSNGLQ
                     AASATLNLVKS"
     gene            10702..11865
                     /gene="galK"
                     /locus_tag="P5335_04215"
     CDS             10702..11865
                     /gene="galK"
                     /locus_tag="P5335_04215"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRZ74116.1"
                     /translation="MKKKLIASTSEHFKTIFQHEPEALFLSPGRINIIGEHVDYNDGF
                     VLPAAINKYICFAVSKSNSDECTLVAKDLNESYKFNWKDDLKPIDKMWVNYILGVLQQ
                     LKERGLPLEGFTIVFSSTIPMGAGLSSSAALECGFGYALNKLFSFHLTKEDLAKIGQK
                     SEHTFVGVKCGIMDQFASVFGKKNKVIKLDCTTLDYEYHKADFKKYSLLLLDSNVKHT
                     HLTSGYNTRRQEVEEGLAIIKNQFPLVKSFRDCTENQLEKLRDELGEILFKRCHFVVN
                     EIRRVNEAVAALDKNDFEKLGQLMYETHDGLSKEYEVSCEEIDFLVASVRHEKSVLGA
                     RMMGGGFGGCSLNLVEKGTEEALITKISEAYLKEFGLTLSAYKVKISKGTTNV"
     gene            11924..12976
                     /locus_tag="P5335_04220"
     CDS             11924..12976
                     /locus_tag="P5335_04220"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HRZ74117.1"
                     /translation="MQTFNTNEHPHRRFNPLTEEWVLVSPHRTKRPWQGQKEKSHSEE
                     LPAYDPNCYLCPGNERSNGTKNPHYTDCFVFENDFAALLQEEVFNENPSKSPLFQLKP
                     ERGINKVVCFSPQHNLTIPEMEVTDITKVIQTWKEQYLELGNHEFINYVQIFENKGAV
                     MGCSNPHPHGQIWAQSSLPTQIEKTQKSLQKYFDANKKSLLKDYLEEELRLRERIVIE
                     NEHFAVVVPFWAIWPYETMIISKRHFGSIIGMTKEETVSFAEIIKGITVKYDNLFETS
                     FPYSSGIHQAPTDRTAHPEWHFHMHFYPPLLRSATVKKFMVGYEMMGEAQRDISPEKS
                     AAILRDLPTLHYKLKQ"
     gene            12993..14657
                     /locus_tag="P5335_04225"
     CDS             12993..14657
                     /locus_tag="P5335_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/sugar symporter"
                     /protein_id="HRZ74118.1"
                     /translation="MNTGFVTLDYVIFIAYALLILGVGLYMSRSKKGHQKNAEDYFLA
                     SKSLPWWAIGTSLIAANISAEQFIGMSGSGFAVGLAISSYEWMAAITLIIVGKYFLPI
                     FIEKGIYTIPEFVEKRFSTNLKTILAVFWVALYVFVNITSVLYLGALAMNTIMGVDMM
                     WAIIGLSLFAAAYSIYGGLASIAWTDAIQVVFLIFGGLATTYLALNTVSDGQGMMEGL
                     GIIYDKAPTKFSMILDESNPEYLNLPGIGVLIGGMWVANLYYWGFNQYIIQRALAAKS
                     LKEAQKGILLAAFLKLLIPFIVVIPGIAAFVIVNDPEMLARLGADGLINIPSDAAADK
                     AYPWLIQFLPSGLKGLAFVALTAAIVSSLASMLNSTSTIFTMDIYKQYINKNSDDKTT
                     VRVGRITSAVALLIGAIVAPLLGGINQAFQFIQEYTGVVSPGILAIFLLGLFWKKTTN
                     KAAIWGALVSIPIAMFFKVGPKGWAEGTWLENIFPSVPFLDQMGYTTLLTMAFIALIS
                     WNQNKGVDDAKGFELRRETFKTSSTFNISAFAILIVLVFLYAFFWK"
     gene            complement(14872..15726)
                     /locus_tag="P5335_04230"
     CDS             complement(14872..15726)
                     /locus_tag="P5335_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Two component regulator three Y domain protein"
                     /protein_id="HRZ74119.1"
                     /translation="MKTITTTFFVFFISFLALAEVSVAEKEALVKFYHTTNGNQWKKT
                     WDLKAATSTWYGVKIKDNKVVGLELPNNKLKGQIPAEISNLKYLETLDLFKNDLNGSL
                     PASIGELQALKTLNVSFNRLTGSIPVTIGNAKSLKTIELYMNSLTGGLPNEIGNLAQL
                     ENLSLFNNMLEGELPKSLFGLKNLKVLMLNSNNFSGKLSYEVENLTALENLSLFENNF
                     VGQVPFELENLSNLKEMNISYNKFNGLISYALAKKDTFQMTMLNDKGLAVRLPLITDN
                     TGVVGTPD"
     gene            16032..17099
                     /locus_tag="P5335_04235"
     CDS             16032..17099
                     /locus_tag="P5335_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRZ74120.1"
                     /translation="MKFKNIFIIILSVGLLTLIVYRITSNKSQSEKNNSKSASPKMAA
                     NVKGLVLTPQKFSNELTLSGTIDANEQIELRSEVSGVVQGIFFKEGQNVTKGQQLIKV
                     NDIELRAQLSQAKTRQSLASENERRAKLLLEKEAISQEEYDVAKAELQSTQAQTQLIQ
                     AQIAKTSIVAPFSGKIGLRSISPGTYVTPTTLIAKLVNNAQVKITFSIPEKYATLMKL
                     NSDLSFTTSGSNEKFSGKIYAMEPAIEQSTRTLQIRALADNKQGRLIPGTYANITLPL
                     DDINDALLVPTEAIIPIQNGKKVFISSKGMAKEVIIETESRTEKEILVTAGLKLGDTV
                     IISGIMALKEGAPLNVTLPKN"
     gene            17105..>20052
                     /locus_tag="P5335_04240"
     CDS             17105..>20052
                     /locus_tag="P5335_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRZ74121.1"
                     /translation="MSLSTLSIKRPVLTIVMNLMIVLFGIIGYTYLGVREFPSIDPAQ
                     ITVQTNYTGANADIIESQITEPLEKAINSIDGIRNISSTSAQGTSVISIEFNLGKNLE
                     EAANDVRDKVSQAVRNLPQDLTAPPVVSKADADSDPILTMTMKSENKNVLELTDYAEN
                     VILQRLQTIPGVSSVRIWGERKYAMRLWIDPIKLSAYGCTASDVLTALNKQNVELPSG
                     KLTGNNTELTVKTLGNLSTEQDFNNIIVKSEGDRIVRLSDVAKASLEAENLETRLSDS
                     GQTMIGLAITPQPGTNYLDISEEFYKQYELLKKDLPKDFELNVAIDTTIFIKKAILEV
                     AETLLISLLLVTLVIYLFFRDWAIAFRPLIDIPVSLIATFFIMYIFGFSINVLTLLAI
                     VLATGLVVDDGIVVTENIFKKVEEGMSPIEAAIKGSNEIFFAVISISITLAAVFLPII
                     FLEGFVGRLFREFGIVIGAAVLISAFVSLSLTPMLNAYLMKGGKQKRSKFYEMTEPFY
                     ENLNKGYANSLGKFMKRKWLSFPILIICLGLSVLFYNILQKETAPYDDRSMILVNVTG
                     QEGATYDYMDRFMQDLSQLITDSIPEKKISLIVTSPGFSAGSLNRGFARLSLVDPEDR
                     DASQKEVADRLTKLTKGFTDTKTSVSEQPTISVNRRGGLPIQFIIQAKNFEMLREKIP
                     EFMEEASKDPTFSITDVNLKFNKPEIYVTINREKAESLGVSVLDVAQSIQLSLSGQRF
                     GYFLMNGKQYQVIGQFDKKDRDQPLDLAAIFVRNNAGQLIQLDNVVTIDEQSSPPQLF
                     HNNRYMAATVSAGLAPGKSIGDGIEAMERIKEKVLDETFTTDLGGESRDFAESNSNTM
                     FAFGLALLLIFLILAGQFESFIDPFIIILTVPMAVAGALFSLWLFGQTWNIFSQIGTV
                     MLIGLVTKNGILIVEFANQLREQGKTKYEAVMQASESRLRPILMTSLTLALGAMPIAV
                     SLGAASQSRMG"
BASE COUNT         6355 a         3729 c         3732 g         6136 t
ORIGIN      
        1 gtagcgggaa caggactcga acctgtgacc ttcgggttat gagcccgacg agctgcctac
       61 tgctctaccc cgcgctgttt cgggtgcaaa tatacaacga aatataagaa agtcaagtct
      121 ttttttaaaa aaatgtgtag tgagttgatt ctttgccctt taaaagtgat tattttttat
      181 ttgctttcgc tttttccttt tccaattttt taaaatagcc ccgcaacaga aaattatgtt
      241 tcatagcttc taaatcctca ttcagtttga ggctggcttg gtttaaattg gtcatggtgg
      301 aatcaatttg tttgactaat ttagggtcat tagacaggta attaagagcc cctttcccct
      361 ctttgatgtt agtaatggtt tcgtttaaat tagtaataac tttttccatt tccttactag
      421 aacggtccaa actgagtacg atctctttga tattggacgc caccgccgta tcttttaaaa
      481 cccccaccac attgtttcgg tcttcaagag aagctatcaa tttattcaag ttttgcacgg
      541 atgctgctgt tcctttcccg gtgattttca ggtaatgcat tgtttctttt aagtcttttg
      601 ccatcagcgt atcgttaatc aagagtccaa ccgtgccttt cccttgcgtg atttctttgg
      661 ttatttttaa taaatcggcc gttaacaatg ccgcattttc gttggtcacg ttgagtgttt
      721 ctaaaatatc atccgttctg atgcggctgt aggtgttgat ttcgtcaccg gattgaaccg
      781 aaagaccctc ccctttgccg ggaatgatgt tgataatcat gtttccgacc aatccgtctg
      841 aggtaatggt ggcaatggca ttttttttaa tgtgtggaat gattttatct tcaatgacca
      901 tcgtcacgcg aatcatcgtg tcgttcagca tggtaatttc gcgaaccgtc cccacgttaa
      961 tccctgaata acggacactg ttgcccactt gcaacccatt gacattattg aaaactgcag
     1021 ttaagtgggt cgtttttccg aacatgtttt gcttattccc aataaaataa atggcggcaa
     1081 caaaaatggc taaccccagg atgacaaaga ttccgagttg aattttttga ttggttgatt
     1141 tttccatagt gtttcttatt taaaaaaggc ttgcactttt tcgtccttta acgctttcaa
     1201 ttcgtcaaaa gtcccttccg cataattggt tccgtcgact aacaaaatca tgcggtttga
     1261 aatcacccgg gcacaatcga catcgtgtgt gatgatgatg gacgaggttt tatattgttg
     1321 ttgaatgttc ttcattaata aaataatttc cttggcggta atcgggtcta aacccgtggt
     1381 gggttcgtcg tataaaatga ttttaggctg caatatcaac gttcgggcta atgccactct
     1441 acgtttcatt ccgccggaaa gttcattggg cattaaatcg atggcatgag ctaagcctac
     1501 attttgcaaa gcttccatca ctaagggcgt cgtatctttt attaccccga attttttggt
     1561 atggcggcgt agcggaaatt ctaaattctc tcgaaccgac atggaatcgt ataaggcact
     1621 cccttggaac aaaaacccga tttcggtgcg aagttcgtct aattcctccc gagagagtgt
     1681 cttgatggct tgtcccatga tgctgatgga accactgtcc ggttcttcta acccaatcag
     1741 gcatttgatc atcaccgatt ttccggagcc tgattttccc atcaccacga ggttttctcc
     1801 ttcgtacaac tttaggttaa aagcatccaa taccttgttg cttccatacg atttgtacag
     1861 attagtgatt tccacgactg gttttctata ttccctaatt tcttccatgg ctataaatca
     1921 taaaaaatat cggttaaaaa aacagctata aagtcaatga taaacaacaa catcgaggta
     1981 tacaccactg ctgaatttgc cgctaaaccc actcctgctg ttcctttttt acaggtataa
     2041 cctttgaaac aacccaccag tccgatggca aaaccaaaaa agaacgattt gagggttgcc
     2101 ggaatgatgt cgccaaattc caaatgttta aatacttgat taaaatataa taaaaaggac
     2161 acgtctcctt tgatattttc tactaaaaaa gagccgtaga tggcaatggc atccccaaag
     2221 aaaaccaaaa taggcaacat caaggtagtc gccatgattc gggtaaccac caaatactta
     2281 aacggattgg tgccggagac ttccatggca tcaatttgtt ccgtgacccg catggagccg
     2341 agctctgccc cgataccgga accaattctg cctgcacaaa ttaacgcggt gatgataggt
     2401 cctatttctc ttatgatgga tatccctacc atagagggaa tccaagatac tgctccaaac
     2461 tcttgaagtg tgggtcggga ttgtaaggta aaaaccagtc cgatgataaa tccggtaacc
     2521 cctaccaaca acagcgaact gttgcccatg ttataacatt gtcggagaaa ttctttaaat
     2581 tcaaaagggc gtttggtcgc ttctttaaaa aaacgggcag aaaaataggc taactctccg
     2641 gtttcttcca ggaatgtttt tgaagtttgg agtattttat caattacttt cataaaaaaa
     2701 tgaattagct gatatgccta aggactactc aaagttaagc cttttctttc actttttcaa
     2761 caatctatct ctacaaaggt attcactaaa agacacaaaa aagttgatgt aagtcatgtc
     2821 cttaaaaatt ctcgtaaaaa cctaccctaa aagttacatc ttgtttactt actcctaaat
     2881 ctagagaaat attagaattg tttattttac tccatttgat acgcaatcct gttccaattg
     2941 ccggattaaa ttttttgaat tgataggtgt caattgcgga aactgaggaa acattcgcaa
     3001 aaaaaactgc tcctaaaaga ccgtctttag taatatccgt tcggtattcc gattccaaat
     3061 aaatcaacgc attactgcga aaccggtttc gggtaaagcc tcttcccgtt ttttgataat
     3121 gatcccaacc aatactgggc aaatcgaggt aatgagcatt gcctccaaac gtattccacc
     3181 aaaacaactt attagcccac acgcgatgct ttgatttaga aaacgacctg taatttctca
     3241 aatcaaaata aacagaacta taatctgtca tattatcatt aaacttggag ttgaatttat
     3301 aatcaaactc cataaacatc cctttggatg gattcgtaat attatccctg gaatcataaa
     3361 tcgtttgaaa tgcaaaacca atggctaatt cgtcgctaaa atcattcttc atgtatttta
     3421 catagtcggt ttctccttca ataaaagatt cctctgaaat attatgataa ttatccaaca
     3481 aaaatcctaa accgaggtat aactgctttt tcacccgacg ggctacatat tgatacaacc
     3541 gccattgttg gtaatctaaa acagattgtt cgttttcgta gttgttagca ccaataccat
     3601 aggtagattg cggataaatc atatagcgat aatcgcccgt taaaatgtat ttgttccctt
     3661 tcgtatacaa atatgtttgc accgggaaaa tatattggtt ggaaaaagtg aacgacggcg
     3721 tgaacgaaat ggtagacata tttgtatttt ccaattcact tagataaaaa gtggttacaa
     3781 atgaaattac gagtgctctt tctgctccgg tagaggagga ggatggtgcc ggaagtaatg
     3841 aaaaaaccgc ttttttatct tgataatctc gctcagaaac taaagaatct tctattttga
     3901 aaactttata atagacatct tttaaatctc tttgttttac gactaatgaa tctgattgag
     3961 tgaaaccact taaataaaat aaagcaagaa aaagaaaaat gcattttgaa atgaaagcct
     4021 tcatttaaag aatcttttaa gggacagccc tacttttccg ttagggaaat ttgtcttgta
     4081 aatttattgc ttttttgaca attatcaata tcctatactt caaaaaaaca atacttagaa
     4141 acttttacaa ggaagcccga atgattaatt ggctttggtt ttccatttgt tcttttttat
     4201 tggtaaaaat catttcagtc aagcgagccc ccattgcttt aaaatcagtt gaaatagtgg
     4261 tgattccatt ttccaccact tttttcaggg gcgtatcgtt gtaggatata attccgaaat
     4321 cttgcccgat acgcaactgt tgttttttgg attgctcaat tacctgaacc aaatggcggt
     4381 cgttcggaat gatgtacaca ttgccgactg cgatggttct tacctcaaaa tcaacaatta
     4441 cttcgtgccc aaaattaaac tccttacaaa atttcaagaa cccttcgacc atgcccaatg
     4501 gttctttaaa tccgggaaat atcaaaatca attgttggta tttatccaat ttttctttgg
     4561 cctttaccaa agcttggtac atgtctttga caaaattttg atgaacagaa ggatactccc
     4621 ttaattcttc gttggtttgg tctaaaatat agacatcatt tttaggcagt gttttgataa
     4681 ggttggctgc tcctactaaa ttggtaggca taatgatgta tttggaataa ttgccgttac
     4741 tttcattgat cagttttcgg aacatttcca aattaaaatg atggaagaaa atatcaatct
     4801 gggctttgtt atcaatggat tccaaaatag aagtatataa atcttcctta aacgcattca
     4861 actcatcaaa aagcacaaaa aaacgttgtt caaaactaaa ttccacactt tttatataat
     4921 agcctttacc gagaatggca aaaacaattc cccttttttt taattcttca tacgccagta
     4981 aaaccgtatc gcgggaaatt gaaaattcta aactcacttt gtttacagaa ggtaacttgt
     5041 ctcctttttg taaccgcttt tctgcaatag ccgcctcgac ggatgcaata atctgtttgt
     5101 attttggaac tcccgaatga ttctctacag aaattagctt cataaaaaat ccatttacta
     5161 aaaactggtg gcaagataat caaaaaccgg taggtaccgg tatggttttt attttttgtt
     5221 tcctacattt gatgacacat tagtactaaa atcatctaaa aaatgcattt ttcacaaata
     5281 gatcacccaa aaaatgtcaa ctctttcgga atccttcctg acgggaaaga ggttttatgt
     5341 tataccctac ggaataaaaa cggtatggaa ttgtcggtta tcaactatgg agcgaccatt
     5401 acctcgttga aattcccgtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 ttagaataca tcagtcaaca tttagaagaa aattatcccg gaatcctaat ggctgaagtg
     5581 acctatatgc taaccgaatc caatcaggta attgtggaat ttcatgctca tagcacggaa
     5641 gataccttat tgaatctaac ccaacacagc tattttaatt tagatggtca ccaaggaaat
     5701 gtgaccaacc aagatttaat cattcattct gaatcggtat tagaaaccaa ccaagaaaac
     5761 atcccgagcg gtgttctgat taagctgagt gacaattcat ttgacttttc taacgcaaaa
     5821 aaagctccca aatccattga taatacgttc actttacaag ccaatcaaaa agttgcggct
     5881 gtgttgacca gtaagttgca taacttaaaa atgactgttc taaccgatca accggcggtt
     5941 catgtctatg ttggcggcaa ttgttttggg caaatcaaag gaaaagaggc ggcggattac
     6001 catccggaaa gtggcatttg ctttgaaaca caaaattatc cggatgctcc gaatcataaa
     6061 aacttccctt ctgccgtttt aaaaaaggga gaacgctatc accatcagac catttttgct
     6121 tttgaaacat tataaaaaaa acactatgaa aaatgtacta acccttttta ctgtcctttt
     6181 gacgaccttc ttcttgggaa gttgttcaga agacgactcg aaaacaccga tagaaccggt
     6241 agtggaagac aattttatcc gtgcggcaga tatgtcttac cttcctttaa ttgaaagtga
     6301 aaatactgtt tattacaatg ccgacaatca gcctgaaata gctttgacca ccttaaaaaa
     6361 tgcgggttgt aataccattc gaattcgtct ttggaaaaat cctgccgatg ctcattccgg
     6421 atttaatgaa gtaaaagcgt ttgctcaacg ggtaaaacaa gccggcatga aagtttggtt
     6481 gaccgtacat tattccgata cgtgggctga ccccggtaat caaactactc ctgttgcctg
     6541 gcaaaactta tcttttgcag atttgaaaac agctgcatca gattatacca caaccatttt
     6601 aacagagatt gaaccggata tctttcaaat aggaaatgaa atcaacagtg ggctgttatg
     6661 gccaaaaggt catttaatca acaatgaaag tcagtgttta gaattgcttt ctacgattag
     6721 ttcccgaatt cgttcgcaat caccaaccac aaaaatcatg ttgcattatg ccggaatcgg
     6781 ttccggtgca acttggtttt ataacaagat gacttcggtt gattatgatt atatcggatt
     6841 atctttttac cctgtttggc atggtaaaag tctaccggat ttaaaaagca ctataaattc
     6901 gttagggcaa acctataaca aaaaggtagt tatcgctgaa acagcttatc cgtttacgtt
     6961 aacttggaat gactggacca ataaccttgt tggtctagaa aaccaattaa ttccagcata
     7021 tcctgctaca ccggaggggc aaaaaaatta ccttttagcc attaaatcac tagtgaagga
     7081 aacaacaagc ggaataggtt ttgcttattg gggaaccgaa tgggttgctt ttagaggtaa
     7141 tgaatctacc aatggttcgc cctttgaaaa tcaagccttg tgggatttta caaacaaagc
     7201 cgtgccggcc atggctgcct ttaagaaaga ttaatcatga aaaagacctt ttattttctg
     7261 cccttatttt taattttatt ccattcaaca ttgaatttcg gacaagcctt ttcaaaagga
     7321 gctgacgtaa gttggttacc ccaaatggaa gctaccgggt atcaatttta tgatgttgac
     7381 ggttccaaaa aagattgttt acaattgttg aaagaccgcg gtatgaatac cgttcgcctt
     7441 cgggtttttg tcaatcctaa tgatgacaaa gccagtggac attgcagcaa agacgaaacc
     7501 gttgcctttg cgttacgggc tcaaaaaatg ggctttcgca ttatgattga ttttcattac
     7561 agcgattctt gggctgaccc tgccaaacaa aacaaaccgg cagcttgggc caagttacct
     7621 tttgatcaat tagcccaagc agtttataac catacgtttg aggtgatgac cgctttaaaa
     7681 aaagcaaaag taaaaaccga atgggttcaa gtaggtaacg aaattcccgg aggcatgctt
     7741 tggcctgacg gcagcactaa caattggaaa cagttgggta ttttgctgaa caaagggtac
     7801 gatgctgtaa aagctgtgga caagtcgatt aaagtgattg ttcatttaga tgagggcgat
     7861 aacaatgcaa aattcagaac gttttttgac aatgctaccg ctcaaaacgt aaaatatgat
     7921 gtgattggac tttcctatta tccgttttgg gtaaaaaaag actataccga aaccattgct
     7981 aatttagcct tcaacttaaa cgatatggtc acccgttaca acaaagaagt gatggttgtt
     8041 gaagttggcg gtgaatatga caaagtgcaa aacaccaaag aattattaga agccaccatc
     8101 aaagcggtca aaaatgtacc taacaacaaa ggtttaggtg ttttgtattg ggagccacaa
     8161 ggtgaaaaaa gttggagtca ttacagttta agtgcttggc aagcggacgg aaaaccctct
     8221 ccggcattag atgcttttaa agaataaaat tatgctaaaa aatttatttc aaccatttgg
     8281 taaattacta ctactataca gtttcgttct cttttccttt gtggggaaag cccaagaaaa
     8341 aattagttta gatgacgatt ggaaattcca ttttggtcat gctgccaatc ctgaaaaaga
     8401 ttttgattac agtaaaaccg ctttacttca caaatcaaac gtttttgcca ctaccattgt
     8461 taacccaaaa tttgtagata ccgcatggac aaaagtcaat gttccacatg attgggtggt
     8521 ggaattaccc tttgtgaaat ctgaacaagt tgaaatggac agtcatggtt acaaacctgt
     8581 aggtggtgct tttccggaaa ccagcattgg atggtataga aaacatttta cggttgacaa
     8641 agcgaaaagc aataaacgtt ttgaaattca atttgacggt atttacagaa atgctgaaat
     8701 ttggttaaac ggattttatg ttggcaccaa ttttagcggt tacgttggta attcctatga
     8761 cgtgtcggac tatgtcaatt ttgaaggtga taatgtactt gtcgttcgtg tggatgccac
     8821 gcaatatgaa ggttggtttt atgaaggagc cggtatttac agacacgttt ggttaaacat
     8881 taccgataaa attttcattc cacaggatgg tgtttttgta cattccgaaa taaagggcaa
     8941 aaatgccatt gtaactattg aaaccaccgt tcaaaataat catttaaaaa ctaccggtgg
     9001 tttggtttac gcctatatta cggatagaaa cgggaaagtt ttagccaaaa cagcagaaca
     9061 aaaaatcacg ttaggaatcc ataaagacat tacggtaaaa cagaaactaa atttaaacaa
     9121 tgtccgcctt tggtcattag aagaccctta tttgtataaa gtaatttcag ttgtaaaatc
     9181 agataaccaa attgtacatc aaactaaaac gcgtttcgga ataaaaacgg taaaatttga
     9241 tgcaaaagaa ggattttttc tcaatggtaa ccatttaaaa atccaaggta ccaataacca
     9301 tcaagaccat gccggaattg ggagtgcctt accggattat atgcagtatt accgcatcaa
     9361 gttattaaaa gaaatgggtt ctaatgccta ccgagccagt cataatgctc caactccgga
     9421 gcttttagaa gcctgtgaca gtttaggaat gttagtggtc gatgaacaac gtttattaaa
     9481 cagcagtccg gaatatattg accaattcaa acgcatactc aaacgcgaca gaaaccatgc
     9541 ctccgtcttt ttatggtcga ttggcaatga agaacaaaat attcaaggaa acgattacgg
     9601 caaaaagatt gcacaaacac ttttagccat tcaagaagat ttagacccca caagaacaag
     9661 cacttacgct gccgatatgg ctaatgattt taaaggggta aacgaagtca ttcccattcg
     9721 tggattcaat tatcgggaat atgccgttgc ggattatcac cgtgatcacc cccatcaacc
     9781 cttgctggga actgaaatgg ggagcactgt tactacccga ggcatctatg aaaaagatga
     9841 aattagggct tatgtacctg atcaggacat cacccatccg tggtgggcaa gcaaggcaga
     9901 aacctggtgg aaattagctg ccgaaaacaa gtattggcta ggtggatttg tatggacagg
     9961 atttgattat cgcggtgaac caactcccta taaatggcca aatatcagtt cgcattttgg
    10021 aattttagat gtctgtggtt ttccaaaaaa catttattat tattaccaaa gttggtggtc
    10081 aacaaaagat gtattacata tttcacccca ttggaattgg cctgaaaaag tggggaaacc
    10141 aattgatgtt tgggtaaatt caaatgccga tgacgtagaa ctctttttga atggcaaaag
    10201 tttgggtaaa aaaacaatgc cccgaaacag tcatttgcaa tgggacgtaa cttatgaacc
    10261 gggtattcta gaagctgtgg gttataaaaa aggaaaaaaa ataaccacaa aagttgaaac
    10321 caccggaaat gcttatgctg tggtgctttc acctgataaa accattttaa cggctgatgg
    10381 aaaagatgca accgtattca acatttcgat tacggatgaa aaaggacgcg aggttcctta
    10441 tgcggacaac atggttaaat tttatatctc cggcgatgct aaaataattg gtgttggcaa
    10501 tggtgatcca agttcacatg agccggataa atgccgggaa ggggcttggc aaagaagtgc
    10561 tttcaacggg aaatgccaag taattattca agccggaaaa acagtaggaa ctgtaaattt
    10621 tgaagccaaa tcaaacgggt tacaagctgc ttcagcaacg ctaaacctag taaaatccta
    10681 aatgaaatct ctttcatcaa aatgaaaaaa aaattaattg cctcgacttc ggaacatttt
    10741 aaaaccattt ttcaacacga gcccgaagct ttgtttctgt caccgggaag aatcaatatt
    10801 attggtgagc atgttgatta taatgatgga tttgtcttac cggcagccat caacaaatac
    10861 atttgttttg ctgtttccaa atcaaattct gacgaatgta cccttgttgc taaagattta
    10921 aatgagtctt ataaattcaa ttggaaagac gatttgaaac ctattgacaa aatgtgggtt
    10981 aattacattc taggtgtttt acaacaattg aaagaacgtg gtttgccttt agaaggtttt
    11041 actatcgtat tcagcagcac tatcccaatg ggagccgggc tatcctcttc cgctgcatta
    11101 gaatgcggat ttggctatgc cttaaacaaa ctattctctt tccatttaac caaagaagat
    11161 ttggctaaaa taggccaaaa gtcagagcac acctttgttg gcgtgaaatg tggcattatg
    11221 gatcaatttg caagtgtttt tgggaagaaa aacaaggtca tcaaattaga ctgtacaacc
    11281 ttagactatg aataccacaa ggcagatttt aaaaaatatt cactactttt attggacagc
    11341 aatgtcaaac atacccatct cacctccggc tataataccc gaagacaaga agtggaagaa
    11401 ggattagcta tcattaaaaa tcaatttcca ttggttaaaa gttttagaga ttgtactgaa
    11461 aatcaattgg aaaaactgag ggatgaatta ggtgaaattc tctttaaacg ctgccatttt
    11521 gtagtaaacg aaattagacg agtgaatgaa gcggttgctg ctttagataa aaatgatttc
    11581 gaaaaattgg gtcaattgat gtatgaaacc cacgatggat tatctaaaga atatgaggtc
    11641 agttgtgaag aaattgattt tttagttgcg tcggttcgcc atgaaaaatc ggttttaggt
    11701 gccagaatga tgggtggcgg atttggcggt tgttccctta atttagtaga aaaaggaaca
    11761 gaagaagcct taattactaa aatttcagaa gcttatttaa aagaatttgg ccttaccctt
    11821 tctgcttata aagtaaaaat ttcaaaagga acaactaacg tataaaataa tagagccgtc
    11881 cataaattgg tccccttttt attgatttct tgaacaccat actatgcaaa ctttcaacac
    11941 aaacgaacat ccccaccgtc ggttcaaccc tttgactgag gaatgggttt tggtatcgcc
    12001 acaccgtacc aaacgccctt ggcaaggaca aaaagaaaaa agtcattcgg aagaattacc
    12061 ggcctatgac cccaattgtt atttgtgtcc gggaaatgaa cgttcaaacg gaaccaagaa
    12121 ccctcattat acggattgtt ttgtgtttga aaatgatttt gccgccttgt tacaagaaga
    12181 agtttttaat gaaaaccctt caaaatctcc cttatttcaa ttgaaacccg aaagaggtat
    12241 caataaagtg gtttgttttt caccccaaca taatttgacc attccggaaa tggaagtgac
    12301 tgatattaca aaagtgattc aaacttggaa agagcaatat cttgaattgg gcaatcatga
    12361 atttatcaat tatgtccaga tttttgaaaa caaaggtgct gtgatgggct gtagtaatcc
    12421 acatccgcat ggacaaattt gggcacaatc ctccttaccc acccaaatag aaaaaacaca
    12481 aaaaagtttg cagaaatatt ttgatgccaa caaaaaaagc ttgttgaaag attatttaga
    12541 agaagaatta agattgagag aaagaatcgt aatagaaaat gagcattttg cagttgtcgt
    12601 acccttttgg gcgatttggc cttatgaaac catgattatc agcaaacgac attttggtag
    12661 tatcattggg atgaccaaag aggagacagt ttcttttgct gaaataataa aaggaattac
    12721 ggttaaatat gataatcttt ttgaaacctc cttcccctat tcttccggaa ttcatcaagc
    12781 tccaacggac cgaacagctc atccggaatg gcattttcac atgcattttt atccgccttt
    12841 gttgcgaagt gccaccgtta aaaaattcat ggtgggctat gaaatgatgg gtgaagccca
    12901 acgtgatatt tcacctgaaa aaagtgccgc catattaaga gacttgccta ccttgcatta
    12961 taaattaaaa caataacact aatccatttc ctatgaatac aggttttgtt accttagact
    13021 atgtcatttt tattgcttac gcacttttaa ttttgggtgt aggattgtac atgtcccgaa
    13081 gcaaaaaagg gcatcaaaaa aatgcagaag attatttttt agccagtaaa tcgctgcctt
    13141 ggtgggctat tggaacttcg ttaattgctg ccaatatttc tgcggaacaa ttcattggca
    13201 tgtcgggttc aggatttgca gtgggtttag ccatttcatc ctatgaatgg atggctgcta
    13261 ttactttaat cattgtaggg aaatatttcc tgccaatatt cattgaaaaa ggaatttata
    13321 ccattccgga gtttgtggaa aaacggtttt ctactaattt aaaaactatt ttggcagtgt
    13381 tttgggtcgc tttatatgtt tttgtcaaca tcacttccgt attatattta ggagccttag
    13441 cgatgaatac gataatgggt gtagacatga tgtgggcaat cattggcctg tcgctttttg
    13501 cagcggctta ttccatttat ggaggtcttg cttctattgc ttggacagat gccattcaag
    13561 tagtgttttt aatttttggt ggattagcaa ccacttacct cgccttaaat actgtttcag
    13621 acgggcaagg tatgatggaa ggattgggaa ttatttatga taaagctcca accaaattct
    13681 cgatgatttt agatgagtcc aatccggagt atcttaattt accgggtatt ggggttttaa
    13741 taggaggaat gtgggtagct aatttatatt attggggttt taaccagtat attatccaac
    13801 gtgcattggc ggcaaaatca ttaaaagaag cccaaaaagg gatattatta gctgcttttt
    13861 taaagttatt aattcccttt attgttgtaa ttccgggaat tgcagccttt gtgattgtaa
    13921 atgatccgga aatgttagcc cgattaggag cagatgggtt aattaatata cccagtgatg
    13981 ctgcggccga taaagcttat ccgtggttga ttcagttttt accttccggt ctgaaaggat
    14041 tggcttttgt agctttgact gctgccatag tttcttcttt agcctccatg ttaaattcaa
    14101 cttcaacgat ttttacgatg gatatttaca aacagtacat caataaaaat tcagatgata
    14161 aaacaactgt ccgagtaggc cgaataactt ctgccgttgc tttattgatt ggagcaatcg
    14221 ttgccccgtt gttaggcgga attaaccaag cttttcagtt tattcaagaa tataccggcg
    14281 tggtgagtcc cggcatatta gccatctttc tattagggct attctggaaa aaaacgacca
    14341 acaaagcagc catttgggga gcattagttt ccatcccgat tgcgatgttc tttaaagttg
    14401 gaccaaaagg atgggctgaa ggcacttggt tagaaaatat tttccctagt gtccctttct
    14461 tagatcaaat gggctatacc actttactta ctatggcttt tattgcttta ataagctgga
    14521 atcaaaataa aggcgttgat gatgccaaag gatttgaatt acgcagagaa acctttaaaa
    14581 caagttctac ttttaacatc agtgcctttg ccatcctgat tgttttagtg ttcctatatg
    14641 ccttcttttg gaaataaacc taagcttgaa tacaaaagtt ttgttagtgc ttaaatctat
    14701 ttgccacaca ttacataaat aaatttgtgt aatgtgtggc attaaaaaaa gtatcagttg
    14761 atacgggaaa cttttatagt atagtatctt aaactacaat ttaaaccaaa aaaaaactca
    14821 caatttgggg atagtaagtt ttttttgggt tgttggtatt tgtattggaa attaatccgg
    14881 agtacctact actccagtat tgtcagtaat caacggtaaa cgaaccgcta atcctttatc
    14941 gtttaacatg gtcatttgga atgtatcttt tttagctagt gcatacgata tcaatccatt
    15001 gaatttgtta taggaaatat tcatttcttt taaattgctt aaattctcta actcaaaagg
    15061 cacttgtcct acaaagttat tttcaaataa gcttaagttt tcaagagctg ttaagttttc
    15121 aacttcataa ctcaatttac cggagaagtt attgctgttt aacatcaata cttttaaatt
    15181 tttcaatccg aataatgatt taggtaattc cccttccaac atattattga ataaagataa
    15241 attttctaat tgagctaaat ttccaatttc atttggcaat ccacctgtta aagaattcat
    15301 gtataactca atcgttttta atgattttgc atttcctatt gtcacaggaa ttgaaccggt
    15361 taagcgatta aacgaaacat ttaatgtttt taacgcttgc aattcaccaa ttgaagccgg
    15421 caaactacca tttaaatcat ttttaaataa atctaatgtt tctaaatatt ttaaattaga
    15481 aatttctgcc ggaatttggc cttttaattt gttgtttggc aattcaagac caactacttt
    15541 gttgtctttg attttcacac cataccaagt agaagtagcc gcttttaaat cccatgtttt
    15601 tttccattga ttaccatttg ttgtgtgata aaatttaact aaagcctctt tttcagcaac
    15661 agaaacttct gctaaagcga gaaatgaaat gaaaaacaca aagaatgtag tagtaattgt
    15721 tttcataagt ttattaaatt tgggtttgat ggagtaaatg taaatacttt tatcgttata
    15781 atacatattt aatcgatgaa atgcgttaat tgttattaaa atatagtttt tacttacaat
    15841 tatcgacgaa ctgcactagg tactttctgt tttaagaaac ctgtttaagt aaaaacaaaa
    15901 cccactcttt ataagccttt aaaaaattaa ctaaatattt gttaaatatc aattcacctt
    15961 aaaacctttg gttttagaaa aagtaaattt gtaaaccata aattttacca ccgtaaaaca
    16021 agcatcacct tatgaaattt aaaaacatct ttataattat actttcagta ggattattaa
    16081 cgctaatcgt ttatagaatt acaagcaata aatcgcaaag tgaaaagaat aacagtaaaa
    16141 gtgcctctcc aaaaatggcg gccaatgtaa aaggtcttgt tttgactcct cagaaatttt
    16201 ccaatgaatt aactttgtca ggtaccattg atgctaacga acaaattgaa cttcgcagtg
    16261 aagtttcagg tgttgtacaa ggtatctttt ttaaagaagg ccaaaacgta accaaaggac
    16321 aacagttaat taaagtcaac gatatcgaat taagagctca attgtcgcaa gctaaaaccc
    16381 gacaatcctt agcttctgaa aacgaacgaa gagccaaatt acttttagaa aaagaagcca
    16441 ttagtcagga agaatacgat gtagccaaag cagaattaca atccactcaa gcacaaaccc
    16501 aattaattca agctcaaatt gctaaaactt cgattgtagc acccttttcc ggaaaaatcg
    16561 gtttacggtc tatttctcca ggaacctatg taacaccaac cacactaatt gcaaaactgg
    16621 tgaacaatgc acaggtaaaa atcacctttt ccattccgga aaaatatgcc acattaatga
    16681 agctgaattc cgatttaagt tttacaacct ccggttccaa tgaaaagttt tcgggtaaaa
    16741 tttatgccat ggaacctgcc attgaacaat ccaccagaac attacaaata agagctttgg
    16801 cagacaacaa acaaggaaga ttaattcccg gcacctatgc taatatcacc ttgcctttag
    16861 atgatatcaa cgatgcctta cttgttccga ctgaagcaat cattccgatt caaaacggta
    16921 aaaaagtttt tatctcttcc aaaggaatgg ctaaagaagt aattattgaa acggaatccc
    16981 gaacagaaaa agaaattctg gtcactgccg gattaaaact cggtgatacg gtcatcatat
    17041 ccggaatcat ggctttaaaa gaaggagcac cgctaaatgt aacattacct aaaaactaaa
    17101 tcgcatgagt ttatcaactt taagcatcaa gcgacccgtt ttaaccattg tgatgaatct
    17161 gatgattgtc ctttttggga tcatcggcta cacttatttg ggtgttcgag aatttccttc
    17221 catcgatccg gctcaaatta cagttcaaac caattatacc ggtgccaacg ctgatattat
    17281 cgaatcgcaa ataacggaac ctcttgaaaa ggcaattaat tccatcgatg gaatccgaaa
    17341 catttcttct accagtgctc aaggtacaag tgttatttct atcgaattta atcttggaaa
    17401 aaaccttgaa gaagcagcta atgatgtgcg tgataaagtt tcgcaagccg ttcgaaattt
    17461 gccacaagat ttaactgcac ccccagtagt ttcaaaagct gatgccgact ccgacccgat
    17521 tctgacaatg accatgaaga gtgaaaacaa aaatgtttta gaactcaccg attatgcgga
    17581 aaatgtaatt ttacaacgtt tgcaaaccat accgggtgtt agtagtgttc ggatttgggg
    17641 ggaacgaaaa tatgccatgc gtttgtggat tgacccaatt aaactttctg catatggttg
    17701 cactgcttct gatgtactaa cggctttgaa caaacaaaat gtcgaattac cctccggaaa
    17761 gctgacagga aacaatactg aattaactgt aaaaaccctt ggtaatttat ctactgaaca
    17821 agattttaac aacatcattg taaaatcaga aggcgataga attgttcgct tgtcggatgt
    17881 tgccaaagct agccttgaag cagaaaacct ggaaacccga ttgagtgatt ccggtcaaac
    17941 catgattgga ctagccatta cgccacaacc gggtaccaat tatttagata tttcggaaga
    18001 attctacaaa caatacgaat tactgaaaaa agatttacca aaagattttg aactgaatgt
    18061 agcgattgac acgaccattt ttatcaaaaa agctatttta gaagtagccg aaactttatt
    18121 gatttcttta cttttagtaa ctttagtaat ctatcttttc ttcagagatt gggccattgc
    18181 ttttcgtcca ttaattgata ttcccgtttc cttaattgct acttttttca ttatgtacat
    18241 ttttggtttt tccatcaatg tacttacgtt gttggcaatt gttttagcaa cgggacttgt
    18301 ggtagatgac ggtattgtag tcaccgaaaa cattttcaag aaagtagaag aaggcatgtc
    18361 gccaatcgaa gccgccatca aaggttccaa tgaaattttc tttgctgtaa tttcaatttc
    18421 catcacgctg gcagccgtat ttcttcccat tatattttta gaaggatttg tgggaagatt
    18481 attccgagag tttggaatcg tgatcggtgc ggccgtgtta atatcggcct ttgtttcttt
    18541 aagtttaacg cccatgttga atgcctattt aatgaaaggc gggaaacaaa aacgttcgaa
    18601 attctacgaa atgaccgaac ctttttatga aaatttaaat aaagggtatg ccaactcgtt
    18661 gggtaaattc atgaagagaa aatggttgag tttcccgatt ttgattatct gtttgggatt
    18721 atcagtcttg ttttacaaca ttctacaaaa agaaacagct ccgtatgacg accgaagtat
    18781 gatacttgta aatgttaccg gacaagaagg tgctacttat gattacatgg atcgctttat
    18841 gcaagatttg tcgcaattga taactgattc cattcctgag aaaaaaataa gtttgattgt
    18901 cacttccccc ggatttagtg caggttctct taacagaggt tttgccagac tttccttagt
    18961 tgatcctgaa gatagagatg cttcgcaaaa agaagttgct gaccgattaa caaaattaac
    19021 caaaggtttt actgatacta aaacatccgt ttccgagcaa cccaccattt ctgttaacag
    19081 acgtggagga ttacccattc agtttattat tcaagcgaaa aactttgaaa tgctaaggga
    19141 aaaaattccg gaattcatgg aagaagcctc taaagaccct actttttcaa ttaccgatgt
    19201 aaacttaaaa ttcaacaaac ctgaaattta tgttactatc aatcgcgaaa aagcggaatc
    19261 attgggtgtt tctgttctcg atgtagccca aagcatccaa ttatcgttaa gcgggcaacg
    19321 ttttggttat ttcttaatga acggaaaaca ataccaagtc attggacaat ttgacaaaaa
    19381 ggaccgtgac caaccgttgg atttagcagc aatctttgtt cgaaataatg ccggtcaatt
    19441 gatacaatta gataatgtag tgactattga cgagcaaagc agtccgccgc aattgtttca
    19501 caataaccgt tatatggcgg caactgtttc agcaggttta gctccgggaa aaagtattgg
    19561 ggacggaatt gaagctatgg aacgcatcaa agaaaaagtg ctggacgaaa cctttacaac
    19621 agatttagga ggcgaatcac gtgattttgc ggagagtaat tccaatacga tgtttgcttt
    19681 cggattagca ttacttctga ttttcctaat cttagcagga caatttgaaa gttttatcga
    19741 tccgtttatc atcattttaa cagtaccaat ggccgttgca ggagccttat tctccctttg
    19801 gctttttggg caaacgtgga atattttcag tcagattgga acagtgatgc taattggatt
    19861 agtaaccaaa aacggaattt tgattgttga atttgccaac caacttcgcg aacagggaaa
    19921 aacaaaatat gaagcggtta tgcaagcctc tgaatctcgc ctccgaccga ttttgatgac
    19981 cagtttaacc ttagctttag gagcgatgcc aattgccgtt tcgttagggg ctgcttctca
    20041 gagtagaatg gg
//
LOCUS       DAOTCW010000032        19980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1465_length_19980_cov_29.005370, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000032 DAOTCW010000000
VERSION     DAOTCW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 19980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19980
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_19980_cov_29.005370"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..467)
                     /locus_tag="P5335_04245"
     CDS             complement(159..467)
                     /locus_tag="P5335_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRZ74122.1"
                     /translation="MGLLDMLGFGSKTENVKDFVDRGAVMIDVRTVGEFQQGHIKGAK
                     NIPLDMIFSKVAEIKKLNKPVIVYCRSGMRSSQAASVLKNSGIEVMNGGGYESLESKL
                     "
     gene            564..1139
                     /locus_tag="P5335_04250"
     CDS             564..1139
                     /locus_tag="P5335_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRZ74123.1"
                     /translation="MKITDRKLEIITIAAKLFKEKGYSAVTMRDIAQAMNIKAASLYN
                     HIKSKQEILVLIIIEIAEEFTSLMNEIVSSNTTVVNKIERVIQLHIDITLRNADALAC
                     LNNDWMHLTDEELNYFVKMRAEYEENFRKIINEGVLTGELKNSNQEVVVFSILSVLRT
                     MYLWHDKTKVLDAQTLKNTITTVFLSGVKNV"
     gene            1252..2325
                     /gene="paaK"
                     /locus_tag="P5335_04255"
     CDS             1252..2325
                     /gene="paaK"
                     /locus_tag="P5335_04255"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HRZ74124.1"
                     /translation="MAKFHSIKVADVYKETKDCTVISFEIPNDLKSEYQFKQGQHLTL
                     RTIIDGNEVRRSYSLCSSPIENKWNVAVKKINEGLFSTFVNEKLRKGDVLDVMAPNGT
                     FFTEIDPKKAKNYIAFAAGSGITPILSIIKTHLALEPESTFKLFYLNRTVKSIVFKEE
                     IEQLKNRYFNRFEIFHFLTKEHRPSELFNGRFTKEKIQTLTDKIIDVPQVDACFICGP
                     EEMIYLLRDELINAGLEKDKIHFELFTSGISEEDKKRISKIIEKKVEGTEVTIIDGGK
                     EFHFVMGDDFDNILDGALAAGADLPFACKGGVCSTCRCKVVEGTTEMKLNYSLEEDEL
                     AKNYILSCQAVPTSKKVVVDFGI"
     gene            2413..3369
                     /gene="paaA"
                     /locus_tag="P5335_04260"
     CDS             2413..3369
                     /gene="paaA"
                     /locus_tag="P5335_04260"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HRZ74125.1"
                     /translation="MSEKEVKSLEEIFDARIARDEKIEAKDWMPEKYRLTHIRQISQH
                     AHSEIVGMLPEANWITRAPSLRRKVALLAKIQDEGGHGLYLYSAAETLGISRDEMYEQ
                     LHTGVAKYSSIFNYPAITWADMGTVGWLVDGAAIINQVALMGASYGPYSRAMVRICKE
                     ESFHQRQGYEILLTLCNGTPEQKAMAQDSLNRWWWPSLMMFGPKDADSPNTEQSVKWK
                     LKRKTNDELRQQFIDQTVPQADLLGLTIPDPKLKWNPKTKKYDWGEIDWNEFWQVVKG
                     HGPCNKQRLAARKAAWEEGTWVRDAAMAYADKQENKVVKEAI"
     gene            3438..3719
                     /gene="paaB"
                     /locus_tag="P5335_04265"
     CDS             3438..3719
                     /gene="paaB"
                     /locus_tag="P5335_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HRZ74126.1"
                     /translation="MKNWPLWEVFIRSKNGLEHRHCGSLHASDATMALENARDVYTRR
                     MEGVSIWVVPSSQITASNPEHNGELFEPAKDKAYRHPTFYELPDELKHM"
     gene            3825..4577
                     /gene="paaC"
                     /locus_tag="P5335_04270"
     CDS             3825..4577
                     /gene="paaC"
                     /locus_tag="P5335_04270"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HRZ74127.1"
                     /translation="MKNNLIQYILGIADNSLILGQRLGELCGHGPSLETDIALTNISL
                     DLLGQTRSYFQYVAKLQGDNATEDTVAFLRLEGDYKNVLLVEQPNTDFGYSIARQFLF
                     DIFHLSLLNELQNSPDETLAAIAKKSIKEVSYHTRFSTDWIRRLGDGTEESHNRIQSA
                     INDLWIFTDELFHQTEADKIMVSEGVGVDVTKLKSDYYKTVNTILKESTLQIPTIEYF
                     QKGGKNGVHSEHMGYILTELQYIQRTYPNMTW"
     gene            4626..5126
                     /gene="paaJ"
                     /locus_tag="P5335_04275"
     CDS             4626..5126
                     /gene="paaJ"
                     /locus_tag="P5335_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HRZ74128.1"
                     /translation="MTEQIQHIDQKLFEILETVSDPEIPVLSIMEMGVVRSAKNENGI
                     VHVQITPTYSGCPAMDVIGDDIKKAFREKGLVAKIELVLSPAWTTDWITPKGRAELEK
                     YGIAAPLSETADKEALLGNKKLVKCPQCGSLNTKLVSQFGSTACKAFFQCEECLEPFD
                     YFKCLK"
     gene            5206..>5860
                     /locus_tag="P5335_04280"
     CDS             5206..>5860
                     /locus_tag="P5335_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRZ74129.1"
                     /translation="MSNSILLKIENKIAFITLNRPDVFNSFNREMALSLQQVIENCKD
                     DTNVRAIVITGNGKAFCAGQDLKEVTDTDLNPGFRKILEEHYNPIIQKIRTIEKPIIA
                     AVNGVAAGAGANIALACDIVLASENASFIQAFSKIGLIPDSAGTFFLPRLIGFQKASA
                     LMMLGDKVSAVEAEKLGMIYKVFAPENFLEEVNKLAETLAEMPTKAIGLTKRLLNQSM
                     "
     assembly_gap    5861..5960
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5961..7020
                     /locus_tag="P5335_04285"
     CDS             <5961..7020
                     /locus_tag="P5335_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRZ74130.1"
                     /translation="NLSALEKSKSSLETTLARLVEKEKIDTKEKNRIENNISFAHSLS
                     ELSHSDLVIEAIVENIDIKRKLFSDLETYVSPETILASNTSSLSIASIAASCQKPERV
                     IGIHFFNPAPLMQLVEVIPAIQTSHEVLTATIQTITDWKKVVAVAKDTPGFIVNRVAR
                     PFYSEALRIYEEGNADFATIDWAMKTIGGFRMGPFELMDFIGNDVNYTVTETVFTAFY
                     FDPRYKPSFTQKRLSEAGFLGRKSGRGFYNYHSELPKPTEDKQLGQYIVDRILVMLIN
                     EAAEAYFLNIASAEDIDNAMTKGVNYPKGLLAWADEKGIKWCVEKLDELYHEYHEDRY
                     RCSPILRKINRENKTFFN"
     gene            7020..7424
                     /locus_tag="P5335_04290"
     CDS             7020..7424
                     /locus_tag="P5335_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRZ74131.1"
                     /translation="MKPIDIVNKMFYNDAFSQWLGIVVEEVSNGSCTLSMTIRKEMTN
                     GFGIAHGGISYSLADSALAFAANSHGRQSVSIDTSVNHIEALKEGDNITAIATENALK
                     NKFGFYTIEIKKEDKIVALFKGTVFRSEKEWE"
     gene            7517..8719
                     /gene="pcaF"
                     /locus_tag="P5335_04295"
     CDS             7517..8719
                     /gene="pcaF"
                     /locus_tag="P5335_04295"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HRZ74132.1"
                     /translation="MEAYIIDGVRTPIGSYQGTLSSVRPDDLGALVIKTVVEKNPNIP
                     KDAYDDVIMGCANQAGEDNRNVARMSLLLAGLPHTVPGETVNRLCSSGLSAIINASRA
                     IKAGDGHVFIAGGIENMTRGPLVVSKPSAAYGTDSKMYDSSFGWRFVNPKMHEMYGTD
                     AMGETAENLADKYAISREDQDKFALNSQQKAAAAQESGRLVKEIVSVEIKQKKGDSYF
                     FHKDEFIKPGTTLEGLSKLRPAFRKDGSVTAGNASGLNDGAAATIIASEEAVKKYNLI
                     PLARIVSSAVVGVEPRIMGIGPVEATNKALTKAGLTLADMDIIELNEAFAAQALACTR
                     AWGIADNDPRLNPNGGSIAIGHPLGVTGARIAYSAALELQLTGKRYALITMCIGVGQG
                     YAAIIERA"
     gene            8772..11267
                     /gene="paaZ"
                     /locus_tag="P5335_04300"
     CDS             8772..11267
                     /gene="paaZ"
                     /locus_tag="P5335_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HRZ74133.1"
                     /translation="MNKTQHYVLGKWTTGTGEGNPSFDAITGEFISSTTVEGLDIPAI
                     LHYGRTKGGEKLRKMTFQERGNMIKSLAMYLTKRKEQFYEISYRTGATRVDSWIDIEG
                     GFGNLYANASLRKLFPNQPYHVEGDPIDLSKGGRFMAHHILVPKKGVAIHINAFNFPI
                     WGMLEKCAVNWMAGMPAVVLPAPTTAYLTEAVVKEIIASNILPEGALQLICGTAKNIF
                     DTVESQDVITFTGSASTGRLLKAHPRIIQESVPFTMEADSLNASILGEDSFPGTPEFD
                     LFVNQARSEMTVKCGQKCTAIRRMIVPEKLIDDVQNALAKSLDKVTIGDPRLKEVRMG
                     ALVNKAQVEVLKNRVNELSKTASVVYGDLDKINIIGADEKGSFVSPILLREDKPFQNL
                     SVHETEAFGPVSTIMPYKNLDEAIELAQMGKGSLVSSIVTNSDEIAKEYVVNAASHHG
                     RILVLNRENAKQSTGHGSPLPLLVHGGPGRAGGGEEMGGVRGIKHYMQRCAIQGSPTT
                     LTEITGIYQPNSAYKEITQHPFQYHWEDIQPGMSLKTHKRTVTDTDIINFANLTWDHF
                     YAHTDITSLDGSIFEKRTAHGYFILAAAAGLFVYPNKGPVAANYGLEECRFLRPIYHN
                     DTIYVRLTCKQKIERDVASAEHPSGIVKWFVEVFDAANELVAVATILTMVQKKQEVFV
                     EMTDERIVECLTKLTYETKPQWGILTPQHLLEHLEHGYKIMSGQMQDFEIATPEKILD
                     KVHHSLYNYEKFPMGTKFPAMKRDELEDLIHPDFETAKAKFFEAREAYKAFFNENPDA
                     VLKNMVFGHLNKYESYLLERKHLNHHFEQFNIL"
     gene            11343..12095
                     /locus_tag="P5335_04305"
     CDS             11343..12095
                     /locus_tag="P5335_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRZ74134.1"
                     /translation="MTEPYVKYNIENNIATIEFFHPEQNSLPSDVLKDLANTITEVGN
                     KHDVKVIILKSGGDRTFCAGASFKELIAINDAETGKVFFSGFANVINAMRKCPKFIIG
                     RIQGKTVGGGVGIAASTDYCMATKFASIKLSELNVGIGPFVVSPAIERKMGVSAMSQI
                     AIDANNFYEANWAREKGLFASVFESIEELDDAVQKFAEHLCTYNPEAMLEMKKVFWRG
                     TEDWDNLLAERAAISGRLVLSDFTKETLKKFK"
     assembly_gap    12096..12195
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12272..12868
                     /locus_tag="P5335_04310"
     CDS             12272..12868
                     /locus_tag="P5335_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HRZ74135.1"
                     /translation="MIYEFKGFIPIIHESSFIHPQAAVTGNVIIGKNVYVGPGAAIRG
                     DWGEIIIEDGCNVQENCTIHMFPGKSMVLKEGAHIGHGAIIHGANIGRNCLVGMNAVI
                     MDDVNVGDECIIGALSFIKAGMQIPNRKMVVGNPAIIIKDVSDEMIDWKTKGTALYQQ
                     LPKECYETLKVVEPLREIPENRPTQEAFYKTLEEFKKK"
     gene            12931..14214
                     /locus_tag="P5335_04315"
     CDS             12931..14214
                     /locus_tag="P5335_04315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRZ74136.1"
                     /translation="MSIIEFKMPKMGESISEATIINWLKNVGDYIEAEETLLEVATDK
                     VDSEVPSPVSGTITEIRFQKDDVVEVGTVLALIDPNEKQETRSKSQEFTSAKFESQPA
                     VTKQTNIDFKQVKTNPDAFLSPLIISIAQKENLSLEEVQSIPGSGNDGRIQKSDVFNY
                     LKNRKYPLQSKPITDSRQPTTFPKPKINFVEGKDRIVEMDRMRKMIADHMVYSKQTSP
                     HVTSYLEVDVTNLVNWRNENKDTFQEKYGEKLTFTPIFIDAVAKAIQDFPMINVSVDE
                     NKIIVHNDINIGMATALPTGNLIVSVVKKANEKDMISIAKEVNYLAESARNNKLKPED
                     IQGSTFTISNVGTFGSLMGTPIINQPEVAILALGIIKKRPEVITTENGDEIAIRSMMF
                     LSLSFDHRVVDGFLGGSFLKRVGDYLEQFDCNTKI"
     gene            14258..15322
                     /locus_tag="P5335_04320"
     CDS             14258..15322
                     /locus_tag="P5335_04320"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRZ74137.1"
                     /translation="MQEVMTAIKVTKAAHSTVGEVDLNNIVMGFKFTDHMFVCDYQNG
                     EWINPRIEPLQLIPTHPAAMALHYGQAIFEGMKATLGKEGTPLLFRPYENAKRLNFSA
                     NRMGMPTFPEEMFVEALKQFTALEKQWIPTKEGSALYLRPFMYADEPFIGMRAATSFK
                     FMVIASPAGPFFSRKIKLYAEKKYVRAVNGGTGEAKAAGNYAAAIRPTEYAKAKSYDQ
                     VLWLDAHDFEYIQEVGTMNIFFKIDGKFITPDLSGSILSGITRMSVIDLLRSKGFEVI
                     EKPIKISEIIEASKNGTLEEAFGTGTAVGIAMIDEIGNNDFTIKLPENNPVSVMVNDT
                     LNAIKLQKEEDQFGWIVEAK"
     gene            15423..17468
                     /locus_tag="P5335_04325"
     CDS             15423..17468
                     /locus_tag="P5335_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRZ74138.1"
                     /translation="MLDKNILKKAFSTMATAKSMAELYEDNFKIVSKYVHATSRGHEA
                     IQIAMALHLLPQDYAFPYYRDDAMMLGIGMRPYDMMLQLMAKKDDPFSGGRTYYCHPS
                     LKDADKPKIPHQSSATGMQAIPATGAAMGFWYKENIGIQEDIELKPIVVCSLGDASVT
                     EGEIAEAFQMAALKQLPILYLVQDNGWDISANAAETRAQNAFEYAKGFNGIETISIDG
                     ANFTESYLALEKVVKTIREERRPFLVHAKVPLLNHHTSGVRMEWYRDDLEEAKSRDPY
                     PVLIQQMLQAGITQQEIDKQLALALATVQEDFEKAQQAEDPKPEDLFTHDFAPTPIIE
                     EVGERNPSREEKVVMVDCALFAVEELMKNHPECLLYGQDVGGRLGGVFREAATLAQKF
                     GDERVFNTPIQEAFIVGSTVGMSAVGLKPIVEVQFADYIWPGLNQLFTEVSRSCYLSN
                     GKWPVSMILRVPIGAYGSGGPYHSSSVESVLTNIRGIKIAYPSNGADLKGLLKAAYYD
                     PNPVVILEHKGLYWSKVKGTDAARVNEPSEDYLIPFGKANIVQEIWEQETAETLTVIT
                     YGMGVHWALNASATIKNQVEIIDLRTLFPLDEETVFKSVKKAKKCLIVTEEPTNNSFA
                     RSLAGLIQEKCFKYLDAPVLVIGSENMPAIPLNSTLEFTMIPNVDKVRAKMEELLEY"
     gene            complement(17749..18282)
                     /locus_tag="P5335_04330"
     CDS             complement(17749..18282)
                     /locus_tag="P5335_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HRZ74139.1"
                     /translation="MKTLKFLPLLLLFLFTSCSSVKVNYDYDKNVDFNQFKTYAFQKS
                     GIDKVEISDLDKKRILRAIDEQMAAKGFTKSENPDLLVNIFTKEREQVDVNQFNAGWG
                     YGWGYGWNPYLWGGNTSVSSYVEGTLFIDLIDAKKKELIWQGQGVGVLTQNMDKKEEL
                     IKEFVTKSLAQYPPDKK"
     gene            complement(18386..>19980)
                     /locus_tag="P5335_04335"
     CDS             complement(18386..>19980)
                     /locus_tag="P5335_04335"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRZ74140.1"
                     /translation="AVFQNWAQYRLTMKYLSEMTEEQTLVMYSGHPMGLFPSHKEAPR
                     VVVTNGMVIPNYSKPDDWEKMNALGVSQYGQMTAGSYMYIGPQGIVHGTTITVLNGFR
                     KIKKNPEGSIFVTSGLGGMSGAQPKAGNIAGCITICAEVNPKITKIRHEQGWINEVIE
                     KVEDLVIRVRKAQAQKEVVSIAYLGNIVDVWERFDSENIHIDLGSDQTSLHNPWAGGY
                     YPVGLSFEESNELMAINPEMFKTKVKETLVRHAAAINKHTAKGTYFFDYGNAFLLEAS
                     RAGADVMAIDGIHFKYPSYVQDIMGPMCFDYGFGPFRWVCASGKPEDLAKTDQIACAV
                     LEEMAKTAPIEIQQQMQDNIKWIKEAQKNKLVVGSQARILYADAEGRMKIAEAFNNAI
                     AKKEIGYVILGRDHHDVSGTDSPYRETSNIYDGSSFTADMAIHNVIGDSFRGATWVSI
                     HNGGGVGWGEVINGGFGMVLDGTEDSERRLKSMLFWDVNNGIARRNWARNEGAIFAIK
                     RAMETQPLLKITIPNLVNEDIL"
BASE COUNT         6616 a         3527 c         3927 g         5710 t
ORIGIN      
        1 caaatcattt aatatgagtg tgctttcgta tgaagtggta gatgatttga gatagtttta
       61 gctgaccaaa caaaaagaac tctgaaaaat aatttcagag ctcttttatt ttaatcaatt
      121 tatttaaaaa acggcttcaa aacagaagcc attttttatt ataatttact ttctaagctt
      181 tcatatccgc caccattcat cacttctatt ccgctgtttt ttaatacaga agctgcttga
      241 ctgcttcgca taccacttct acagtaaaca atgaccggtt tgttcaattt tttgatttcc
      301 gctaccttcg aaaaaatcat gtccaacgga atgttttttg cccctttgat gtgcccttgc
      361 tgaaattccc caacggttct tacatcaatc ataacagctc ctctgtccac aaaatcttta
      421 acattttcgg ttttagaacc aaaacctaac atatctaata atcccataat tttaaatttt
      481 atgggacaaa gataatagct gccaacagtt taatcggtaa caaaagttac gcaactgaaa
      541 atattctagt atatttgatt tatatgaaga ttaccgatcg aaaattagag attattacaa
      601 ttgctgctaa actttttaaa gaaaaaggat acagtgccgt gaccatgcgt gatattgcac
      661 aagcgatgaa catcaaagca gccagtttgt ataatcacat aaagtcaaag caagaaatac
      721 tagttttgat tattattgaa attgctgaag agtttaccag cttaatgaat gaaatagtta
      781 gctcaaacac gacagttgta aataaaatcg aacgggtcat tcaattgcac attgatatta
      841 cgctcagaaa tgccgatgca ttggcctgtt tgaacaacga ttggatgcat ttaaccgatg
      901 aagagctgaa ctattttgtt aaaatgcggg ccgaatatga agagaatttt cgaaaaatta
      961 ttaacgaagg tgttttaaca ggcgaattga aaaacagtaa tcaggaagta gtcgttttct
     1021 caatattatc tgtacttcga actatgtatt tgtggcatga taaaacaaaa gttctagatg
     1081 ctcaaacact taaaaacaca ataactaccg tttttttaag tggggttaaa aacgtataga
     1141 tactcctagt ggacagctgt taattttttc aaacgttttc gtaacttatg aaaaaaatga
     1201 ttaaatttgc attgtaaact aacagttgtt agtttttatt aattataaaa gatggccaaa
     1261 tttcatagta taaaagttgc ggatgtttat aaagaaacaa aggattgtac agtaatttcc
     1321 tttgaaattc ccaatgattt aaagtcagaa tatcagttca agcaaggaca gcatttaact
     1381 ttacgaacaa taattgacgg taatgaagtg cgtcgttcgt attcgctctg ttctagtccg
     1441 attgaaaata aatggaacgt agctgtaaaa aaaataaatg aaggactgtt ttctacattt
     1501 gtcaatgaaa aattacgaaa aggagatgtt cttgatgtaa tggctcctaa tggtactttt
     1561 tttaccgaaa ttgatccaaa aaaagcaaag aattatattg catttgctgc cggaagtggc
     1621 attacaccga tactttcgat tatcaaaaca catttggcgt tagaacctga atcgactttt
     1681 aaattgtttt atttgaatag gactgttaag tcaattgttt ttaaagaaga aattgagcaa
     1741 ttaaaaaaca gatattttaa tcgattcgaa atttttcatt ttttaacgaa agaacatcga
     1801 ccttccgaat tgtttaatgg tcgttttacc aaagaaaaaa tccaaacttt aaccgataaa
     1861 atcattgatg ttccgcaagt agatgcttgt tttatttgtg gtccagagga aatgatttat
     1921 ttgctccgcg atgaattgat taatgctggt ttagaaaaag ataaaattca ttttgaattg
     1981 tttacgtccg gtatttcaga ggaagataaa aaacgtatct ctaaaattat tgagaaaaaa
     2041 gtagaaggaa ccgaagtaac gattatcgat ggcggtaaag aattccattt tgtcatgggt
     2101 gatgattttg ataacatttt agatggtgct ttagcagccg gagccgattt gccttttgca
     2161 tgcaaaggag gcgtttgtag tacatgtcga tgcaaagtag tagaaggaac cacagaaatg
     2221 aaactcaatt attcgctcga agaagacgaa ttggctaaga attatatatt aagttgtcaa
     2281 gccgttccaa catctaaaaa ggttgttgta gattttggaa tttaaaaata ttaaaattgt
     2341 ttaacagttc aacgttactg ctaagtagaa actgtatgct gagactgaac actgaacact
     2401 attaaaaatg ttatgtcaga aaaagaagta aaaagtttag aagaaatttt cgatgctcgc
     2461 attgcacgag atgaaaaaat cgaagccaaa gattggatgc ccgaaaaata ccggttgacg
     2521 catattcgtc aaatatcaca acatgcacat tccgaaattg ttgggatgtt gccggaagcc
     2581 aattggatta ccagagcacc ttctctacga agaaaagttg ccttattagc taagattcaa
     2641 gacgaaggcg gtcatggttt gtatctatac agtgctgcgg aaacattagg aatttcccgc
     2701 gatgaaatgt atgaacaatt acacaccggt gtggcaaaat actctagtat ttttaattat
     2761 cctgctataa catgggctga tatgggtacg gtaggttggt tggttgatgg tgcagcaatt
     2821 atcaatcaag tagctttaat gggagcatct tacggacctt attcacgagc aatggttcgt
     2881 atttgcaaag aagaaagttt tcaccaacgc caaggttatg aaattcttct aacactttgc
     2941 aatggaactc cggagcaaaa agcgatggca caggattctt tgaatcgctg gtggtggcca
     3001 tctttaatga tgtttggacc caaagatgca gattctccaa atacagagca atctgtaaaa
     3061 tggaaattga aacgtaaaac aaatgacgaa ttacgccaac agtttatcga tcaaacagtt
     3121 ccgcaggctg atttattagg attaaccatt cctgatccaa aattaaaatg gaatccgaaa
     3181 accaagaagt atgattgggg cgaaattgat tggaatgaat tttggcaagt agtaaaaggt
     3241 cacggacctt gcaataaaca acgtcttgcg gctcgaaaag cggcatggga agaaggaaca
     3301 tgggttcgcg atgctgcgat ggcttatgcc gataaacaag aaaataaagt agtaaaagaa
     3361 gcaatataaa tagtcacaag tcatagtttt cagtattcag actgcaaact gcgactgcga
     3421 ctgtaaactt aaagaaaatg aaaaactggc cactttggga agtatttata cgaagtaaaa
     3481 acggattaga gcaccgccat tgcggtagtc ttcatgcaag tgatgcaaca atggcactag
     3541 aaaatgcacg agatgtgtat acacgaagaa tggaaggcgt gagtatttgg gtggttccat
     3601 ccagtcaaat tacagcttca aatccggagc ataatggcga attatttgag ccagcaaaag
     3661 ataaggcgta tcgccatcct actttttatg aattgccgga cgaattaaaa catatgtaat
     3721 caatctacaa ggtttttaaa accttgtagg tttatactat aatttgcgaa tttgcggtaa
     3781 aatagtcttt gctacgctct gactttgcga gaaaaaaaca atttatgaaa aacaatttaa
     3841 ttcaatacat cctcggaata gccgataatt cattgatttt aggtcaacgt ctaggtgaat
     3901 tatgtggtca cgggcccagt ttagaaacgg atatagctct aacaaatatt tcattggatt
     3961 tgttaggtca aacgcgaagt tattttcagt atgtcgctaa attacaagga gataatgcca
     4021 cagaagacac tgttgctttt ctaaggctag agggtgatta caaaaatgta cttttagttg
     4081 aacaaccgaa tactgatttt ggctattcaa tcgccagaca atttctgttt gatatttttc
     4141 atttgtcttt attaaatgaa ctgcaaaaca gtcctgacga aacattggct gcgattgcta
     4201 aaaaaagcat caaagaagtt tcgtatcaca cccgattttc aacggattgg atcagaagat
     4261 taggtgatgg aaccgaagag agtcacaata gaattcaatc tgcaatcaac gatttgtgga
     4321 tttttacaga cgaattattt catcaaacag aagctgataa aataatggtt tctgaaggag
     4381 ttggtgtgga tgtaacaaaa ttaaaaagcg attattataa aactgtaaat acaattttaa
     4441 aagaatcaac tttgcaaata ccaaccattg aatactttca aaaaggagga aagaatggag
     4501 ttcacagcga acatatgggt tatattttga ccgaattaca atacatacaa cgcacttatc
     4561 ccaatatgac ttggtaaaat atgatttgtc attctgaact tgtttcagaa tcgcgtaaaa
     4621 caaatatgac agaacaaatt cagcatatcg accaaaaatt attcgaaatc cttgaaactg
     4681 tttccgatcc ggaaataccg gttttgtcga ttatggaaat gggtgtggtt cgttccgcaa
     4741 aaaatgaaaa tggaattgtt catgttcaaa taacaccgac ttatagcggt tgtcccgcaa
     4801 tggatgttat tggagatgat ataaaaaaag catttcgcga aaaagggcta gtggccaaaa
     4861 tagaattagt tctttctccg gcttggacaa ccgattggat tacacccaag ggaagagccg
     4921 aattagaaaa atacggaata gccgctccat taagcgaaac agcagataaa gaggcccttt
     4981 tgggaaataa aaaattagta aaatgtccac aatgtggttc attaaatact aagttagtca
     5041 gtcagttcgg ttccactgca tgtaaagcat tttttcaatg cgaagaatgt ttagaaccat
     5101 ttgactattt caaatgttta aaataataat ttgcaaatgt gtggttcgta gtttttgcga
     5161 caccgctccc gatagttatc gggattgcga gaaacaaaat aaggtatgag caattcaatt
     5221 ctcttgaaaa ttgaaaacaa aatcgcattt attacgctga atcgtccaga tgtatttaat
     5281 agcttcaacc gtgaaatggc tttgtcattg caacaggtta tagaaaattg taaagatgat
     5341 actaacgttc gagccattgt cattaccgga aacggaaaag cattctgtgc cggacaagat
     5401 ttaaaagaag tcaccgacac tgatttaaac cccggtttca gaaaaatttt agaagagcat
     5461 tacaatccaa taattcaaaa aatccgaaca attgaaaaac caattattgc tgctgttaat
     5521 ggtgtagcag ccggagcagg agcaaatatt gctttagctt gtgatatcgt gctagcttct
     5581 gaaaatgctt cttttataca agcttttagc aaaattggat taattcctga tagtgccgga
     5641 acctttttct tgccacgttt aataggattc caaaaagcct ccgctttaat gatgttaggc
     5701 gacaaagttt ctgccgttga agcagaaaaa ttgggaatga tttacaaagt atttgcaccg
     5761 gaaaactttt tagaagaagt gaataagtta gccgaaacgt tagccgaaat gccaactaag
     5821 gccattggtt taaccaaaag attattaaat caatcaatga nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn taatctttca gcacttgaaa aaagtaaatc atctctcgaa
     6001 accactttag caagattagt tgaaaaggaa aaaatagaca ccaaagaaaa aaatcgaatt
     6061 gagaacaata tttcgtttgc acattcttta agcgaattat cccattccga tttagttatt
     6121 gaagctatag ttgaaaatat agacatcaaa agaaaattgt tttccgattt agagacatat
     6181 gtttcacccg aaacgatttt agcttccaat acttcttcat tgtccattgc ttcaattgcc
     6241 gcttcttgtc agaaaccgga acgagtaatt ggaattcatt tctttaatcc cgcaccatta
     6301 atgcaattgg ttgaggtaat accggccatt caaacaagtc acgaagtgtt aacagcaaca
     6361 attcaaacca tcaccgattg gaaaaaagtt gtggctgttg ccaaagatac tcctggtttt
     6421 attgtcaatc gtgtggctcg ccctttttac agtgaagcat tgcggattta cgaagaagga
     6481 aatgctgatt ttgcaaccat cgattgggcg atgaaaacta taggtggttt ccgaatgggc
     6541 ccatttgaac taatggattt tattggtaac gatgtcaatt atacagtcac agaaacggtt
     6601 tttaccgctt tctacttcga tcctagatat aaaccttctt ttacacaaaa aagattgtcc
     6661 gaagcaggtt ttttgggcag aaaatcagga agaggtttct ataattatca ttcagaattg
     6721 ccaaaaccaa ccgaagataa acaactagga caatatatcg ttgacagaat tttagtcatg
     6781 cttatcaacg aagctgccga agcttacttt ttaaacattg cttctgccga agacatcgac
     6841 aatgcaatga ctaaaggtgt aaattatccc aaaggattac tggcttgggc agacgaaaaa
     6901 ggaatcaaat ggtgtgttga aaaattagac gaattgtatc acgaatacca tgaagaccgc
     6961 taccgttgta gtccgatttt aagaaaaatc aacagagaaa ataaaacatt tttcaactaa
     7021 tgaagccaat tgatattgta aataaaatgt tttataacga tgctttcagc cagtggttag
     7081 gaattgtcgt ggaagaagtt tccaatggtt catgtactct ttctatgact atccgaaaag
     7141 agatgaccaa tggttttggt attgctcacg gcggaatttc ctattcgtta gccgacagtg
     7201 ctttggcttt tgcagccaat tcacacggcc gtcaatccgt aagcatcgat acatctgtca
     7261 atcacattga agcattgaaa gaaggggata atattaccgc aattgccact gaaaatgccc
     7321 taaaaaacaa gtttgggttc tataccatag aaataaaaaa agaagataaa atagttgcac
     7381 tttttaaagg aaccgtgttc cgaagtgaaa aagagtggga gtaatagtat aattaacaat
     7441 tagcaattga taattgatta atgaaaggca agaagagaaa attgtcaatt atcaattatc
     7501 cattatcaat aaaaaaatgg aagcatacat catagacgga gttcgtactc caattggaag
     7561 ttaccaagga actttatcat ctgttcgacc agatgattta ggtgcattgg tcataaaaac
     7621 tgttgtagaa aagaatccaa acattccaaa ggatgcctat gacgatgtaa ttatgggctg
     7681 tgccaatcaa gcgggtgaag acaacagaaa tgttgcaaga atgtccctgc ttttagctgg
     7741 attgcctcac actgttcctg gagaaacggt taacagattg tgtagttccg gtttgtcagc
     7801 catcatcaat gcaagtcgtg caatcaaagc tggcgacggt catgttttta ttgccggagg
     7861 aattgaaaat atgactcgcg gacctttagt ggtttctaaa ccatctgccg cttatggcac
     7921 cgatagtaaa atgtatgatt ccagttttgg atggcgtttt gttaatccga aaatgcatga
     7981 gatgtatggg acggatgcta tgggtgaaac tgcagaaaat ttagcagaca aatacgctat
     8041 ttcgcgagaa gatcaagata aatttgcttt gaatagtcaa caaaaagcag ctgcagctca
     8101 ggaatcagga cggttggtta aagaaattgt ttctgtggaa attaaacaaa aaaaggggga
     8161 ttcctatttt ttccacaagg atgaatttat taaacctgga accacattag aaggtttatc
     8221 taaacttcgc ccggctttta gaaaagacgg ttctgttaca gccggaaacg catcaggttt
     8281 aaatgacgga gcagccgcaa ccatcatcgc atccgaagaa gctgtaaaga aatacaattt
     8341 aataccattg gccagaattg tcagttcggc ggtagtaggg gtagagccac gaattatggg
     8401 gatcggaccg gtcgaagcaa ctaataaagc actgacgaaa gcaggtttaa cactagccga
     8461 catggatatt attgaactga atgaagcatt cgctgctcaa gcattagcat gcaccagagc
     8521 ttggggaatt gccgacaacg atcctcgatt aaatccaaat ggaggttcga ttgcaatcgg
     8581 acatcctctt ggtgttacgg gagcaagaat tgcctattcc gccgctttag aattgcaatt
     8641 aactggaaaa agatatgcct taatcactat gtgcattggt gtgggacaag gctatgcagc
     8701 cattatagaa agagcttaaa acttaatgaa ccttaatcgt ttaatggttc aaaaaaataa
     8761 atgttgaaaa tatgaataaa acacaacatt acgtcctcgg aaaatggacc accggaacag
     8821 gcgaaggaaa cccttccttt gatgccatca ccggagagtt catttcttcc acaacagtag
     8881 aaggattaga tattcctgct attttgcatt acggaagaac aaaaggagga gaaaaactcc
     8941 gcaaaatgac cttccaagaa cggggtaata tgattaaaag tttggcgatg tatttaacca
     9001 aacgcaaaga gcaattctac gaaatcagtt accgaacagg agccacacga gtggattctt
     9061 ggattgacat cgaaggtggt tttggtaatt tgtatgccaa tgcttccttg cgaaaattgt
     9121 ttcccaatca accgtaccac gttgaaggag atccaatcga tttgtcgaaa ggaggacgat
     9181 tcatggctca ccatatttta gttcctaaaa aaggagtagc cattcacatc aatgcattca
     9241 atttcccaat ttgggggatg ttagagaaat gtgccgtaaa ctggatggcc ggaatgccag
     9301 cggttgttct gccggctcca acaacagcct atttaactga agcagtggtg aaagaaatca
     9361 tcgcttcaaa cattttgccc gaaggtgctc tacaattgat ttgcggaaca gcaaaaaata
     9421 tttttgacac cgttgaaagt caagacgtga ttacgtttac aggttcagct tcaacaggta
     9481 gattattaaa agcacaccca cgaattattc aggaatctgt tccgtttact atggaagccg
     9541 attcattaaa tgcgtctatt ttaggtgaag attcctttcc gggaacacct gaatttgatt
     9601 tgtttgtgaa tcaagcaaga agtgaaatga ccgtgaaatg tggacagaaa tgtacagcta
     9661 tcagaagaat gatagtgccg gaaaaattaa tcgacgatgt tcaaaatgct cttgcaaaat
     9721 cattagacaa agtcaccatc ggcgacccaa gattgaagga agttcgtatg ggagctcttg
     9781 tcaacaaagc tcaagtagaa gttctgaaaa atagagtaaa cgaattatcc aaaacggcgt
     9841 ctgttgttta cggagattta gataaaataa acattattgg tgccgatgaa aaaggctcat
     9901 ttgtgagccc gattttattg cgagaagaca aaccttttca aaatttgtcg gtgcacgaaa
     9961 ccgaagcatt cggcccggtt tcaaccatca tgccttataa aaatttagac gaagcaatcg
    10021 aattagccca aatgggcaaa ggttctttgg tttcatccat cgttaccaat tcagacgaga
    10081 ttgccaaaga atatgtagtc aatgctgctt cacaccatgg tcgaatttta gtcctaaacc
    10141 gtgaaaacgc taaacaaagt acaggtcatg gttcgccatt gccactatta gttcatggcg
    10201 gtcctggtcg tgcaggagga ggagaagaaa tgggaggtgt tcgaggaatc aaacattata
    10261 tgcaacgctg tgccattcaa ggttctccaa caacattgac cgaaatcacc ggaatttatc
    10321 aaccgaattc ggcgtacaaa gaaattacac aacacccgtt tcaataccat tgggaggata
    10381 ttcaacccgg aatgtcgctc aaaacgcata agcgaacggt cactgatacc gacatcatta
    10441 attttgccaa tttaacgtgg gatcattttt atgctcacac cgacattacc tcattagacg
    10501 gtagtatttt tgaaaaaaga acggctcatg gttatttcat tttagccgct gccgcaggat
    10561 tatttgtata tccaaataaa ggaccggttg ctgccaatta cgggctcgaa gaatgccgat
    10621 ttttacgacc tatttaccac aacgacacca tttatgttcg cctaacgtgc aaacaaaaaa
    10681 ttgaacgcga tgtagcttct gctgagcatc ctagtggaat agtaaaatgg ttcgtggaag
    10741 tttttgatgc agcaaatgaa ttggttgccg ttgccacaat tttgacaatg gttcagaaaa
    10801 aacaagaagt ctttgtcgaa atgaccgatg aaagaattgt agaatgtctt actaaattaa
    10861 cttatgaaac aaagccccaa tggggaattt taacacccca acatttgtta gaacatttgg
    10921 aacatggcta taaaatcatg tcgggacaaa tgcaggattt tgagattgct acaccggaaa
    10981 agattttaga caaagtacat cattcgcttt ataattatga aaaatttcca atgggaacca
    11041 agtttccagc catgaagaga gacgaattag aagacctgat tcatcctgat tttgaaacgg
    11101 ctaaagctaa atttttcgaa gcgagagaag cttataaagc atttttcaat gaaaatccgg
    11161 atgcagtgtt aaagaacatg gttttcggac atttgaataa atacgaatcc tatttactag
    11221 aacgaaaaca tttgaaccat cattttgaac aatttaatat tttataattg agaatgaact
    11281 ttgcggactt tgcgtaaatc ttcgcgtccc gatgtttcgg gattgcgtta aaaatataag
    11341 taatgacaga accctacgta aaatacaata ttgaaaataa tatagccact atcgagtttt
    11401 ttcatcccga acaaaactcg ttgccaagtg atgtgctaaa agatttggca aataccatca
    11461 ccgaagtagg aaacaaacat gacgtaaaag ttattatcct aaaaagtggc ggcgatcgca
    11521 ctttttgtgc cggagccagt tttaaagaat tgattgccat caacgatgcc gaaaccggga
    11581 aagtgttttt ctccggattt gcaaatgtta tcaatgctat gcgtaaatgt ccgaagttca
    11641 tcatcggcag aatacaggga aaaaccgtag gtggaggagt tggtatagct gcttctaccg
    11701 attattgcat ggctactaaa ttcgcttcaa tcaaattaag cgaattgaat gttggaatag
    11761 gaccttttgt ggtttctccg gctatcgaaa gaaaaatggg tgtttcggcc atgtcacaaa
    11821 tcgccataga tgccaataac ttttatgaag ccaattgggc acgagaaaaa ggactatttg
    11881 catctgtttt tgaatctatt gaagagttag acgatgctgt gcaaaaattt gccgagcatt
    11941 tgtgcaccta caatccggag gcaatgctcg aaatgaaaaa agttttttgg agaggaacag
    12001 aagattggga caatctatta gccgaaagag cagccattag cggtcgtttg gttttatcag
    12061 attttacaaa agaaacattg aagaagttta agtaannnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnncggtc gtttggtttt atcagatttt acaaaagaaa cattgaagaa
    12241 gtttaagtaa gcaaatcaca aattacaaaa gatgatctac gaattcaaag ggttcatccc
    12301 cattatacac gaaagtagtt ttatccatcc gcaagcagcg gtaacgggta atgtgattat
    12361 tgggaaaaac gtttatgtgg gcccaggagc tgcaattcga ggcgattggg gtgaaataat
    12421 tattgaagac ggctgtaatg tacaggaaaa ttgcacgatt catatgtttc caggtaagtc
    12481 aatggtttta aaagaaggag cacacattgg tcacggagca attattcacg gagccaatat
    12541 cggcagaaat tgtctagttg gtatgaatgc tgtaataatg gatgatgtga atgtaggcga
    12601 cgaatgcatt attggagcct taagttttat caaagcagga atgcaaattc ctaaccgtaa
    12661 aatggtagtg ggaaatccag caataattat taaagatgtt tccgacgaaa tgattgattg
    12721 gaaaaccaaa ggaacagcct tatatcagca attgccgaaa gaatgttatg aaaccttaaa
    12781 agtagttgaa ccattgaggg aaattcctga aaaccgtcca acacaagaag ccttttataa
    12841 aacattagaa gaatttaaaa aaaagtaatc agtcacaatc acagtattca gatagtactg
    12901 tgaatgagac tgcgactgaa aactaagcat atgtcaataa tcgaattcaa aatgcccaaa
    12961 atgggtgaaa gcatatccga agccaccatc atcaattggt taaaaaacgt tggtgattat
    13021 attgaagcgg aagaaacttt gctggaagtt gccaccgata aagtggacag tgaagtgcca
    13081 tcaccggttt cgggtaccat cactgaaata cgttttcaaa aagatgatgt tgttgaagtg
    13141 ggaactgttt tggctttgat tgatcccaat gagaagcaag agactagaag caagagccaa
    13201 gaatttacta gtgctaaatt tgaatcacaa ccagcggtaa ccaagcaaac gaatattgat
    13261 tttaaacaag taaaaaccaa tccagacgct tttctttcac ccttaatcat ttccatcgcc
    13321 caaaaagaga atctttcttt agaagaagta caaagtattc caggctcagg aaatgacgga
    13381 cgtattcaaa aaagtgatgt gttcaattat ttaaaaaaca gaaaataccc gttacaatca
    13441 aaaccgataa ccgacagccg acaaccgaca acctttccaa aacctaaaat caattttgta
    13501 gaaggaaaag accgaattgt ggaaatggat cgtatgcgaa agatgattgc cgatcacatg
    13561 gtgtattcca agcaaacttc gccacacgta acttcctatt tggaagtcga tgttacaaat
    13621 ttggtgaatt ggcgaaatga aaataaagat acatttcaag agaaatacgg cgaaaaacta
    13681 acatttacac ccatttttat tgatgctgta gcaaaagcca ttcaagactt tccgatgatt
    13741 aatgtttcag ttgatgagaa caaaatcatt gtacacaatg acatcaatat cggaatggca
    13801 accgctctac caacgggaaa tttgattgtt tcagtagtca aaaaagcaaa tgaaaaagac
    13861 atgatttcga ttgctaaaga agtcaattat ttagcagaaa gtgcccgaaa taataaattg
    13921 aaaccggaag acatccaagg aagtacattc accatttcta atgtaggaac atttggaagt
    13981 ttaatgggaa cacctatcat caatcaaccg gaagtagcca ttttagcctt aggaatcatc
    14041 aaaaaaagac cggaagtaat taccacagaa aatggagacg aaatcgccat cagaagtatg
    14101 atgtttttat ccttatcctt cgatcatagg gtagtggatg gatttttagg cggatcattc
    14161 ttaaaacgag tgggcgatta tttagaacag tttgattgta atacaaaaat ataattttct
    14221 cttttcgtaa tttgaattga gagcaaacat aaacaaaatg caagaagtaa tgacagcaat
    14281 taaagtaaca aaagcagcac attccaccgt tggagaagtc gatttaaaca atatcgtgat
    14341 gggattcaaa ttcaccgatc atatgtttgt gtgcgattat caaaacggcg agtggataaa
    14401 tccgcgaatt gaaccattac agcttattcc gacacatcct gctgccatgg ctttgcacta
    14461 tggtcaggcg atttttgaag gaatgaaagc cacgttggga aaagagggaa ctcctttgtt
    14521 attccgtcct tatgaaaatg caaaacgatt aaatttcagt gctaaccgaa tgggaatgcc
    14581 tacttttcca gaggaaatgt tcgtagaagc attaaaacaa ttcaccgcgt tagaaaaaca
    14641 atggattcct actaaagaag gaagtgcttt gtacttgcgt ccgtttatgt atgccgatga
    14701 gccttttatc ggaatgcgtg ccgcaaccag ttttaaattt atggtgattg cttcaccagc
    14761 cggacctttt ttcagtagaa aaattaaatt gtatgccgag aaaaaatatg ttcgtgccgt
    14821 gaatggtgga acaggagaag caaaagcagc agggaattac gccgcagcga ttcgcccaac
    14881 cgaatatgcc aaagcaaaat catatgatca agtattgtgg ctagatgctc acgattttga
    14941 gtacatccaa gaagtgggaa cgatgaatat tttctttaaa attgacggta agttcatcac
    15001 cccagattta agcggttcca ttttatccgg aattacgcga atgagtgtca ttgatttact
    15061 tcgttccaaa ggttttgaag tcattgaaaa accaatcaaa atttccgaaa tcattgaagc
    15121 atccaaaaac ggcactttag aagaagcatt cgggaccggg acagcagtcg gaattgccat
    15181 gattgatgaa atcggaaaca atgattttac gattaaatta ccggaaaata atcccgtttc
    15241 cgtgatggta aatgatacgc tgaatgccat taaactacaa aaagaagaag accaattcgg
    15301 ttggattgtg gaagcaaaat aataagctaa taaagaattt aaaccattaa ggcattaaga
    15361 agaattaaga ataacttaat gaaacttaat ttcttaatgg ttcaaaataa atagttcaat
    15421 aaatgttaga caaaaatatc ctgaaaaaag ccttttcaac catggcaaca gccaaatcaa
    15481 tggctgaatt gtatgaagat aacttcaaaa tcgtttccaa atatgttcac gccacgtcac
    15541 gtggacacga agctattcaa attgctatgg cactgcattt attgccgcaa gattatgctt
    15601 ttccttatta ccgtgacgac gccatgatgc ttggaatagg tatgcgtcca tacgatatga
    15661 tgctccaatt aatggcaaag aaagacgatc ctttttcagg aggacgaacg tattattgtc
    15721 accccagttt aaaagatgct gataaaccaa aaattcctca tcaatcttct gccaccggaa
    15781 tgcaagcgat tcctgcgacc ggagcagcga tgggattttg gtataaagaa aatataggaa
    15841 ttcaagagga cattgagcta aaacccattg tcgtttgttc ccttggcgat gcatcggtaa
    15901 ccgaaggtga aattgccgaa gcctttcaaa tggctgcttt aaaacaatta cctattttat
    15961 atttagtcca agataatggc tgggatattt ctgccaatgc cgccgaaacc cgagctcaaa
    16021 atgcatttga atatgccaaa ggttttaacg gaatcgaaac catttcaatc gacggagcga
    16081 attttaccga aagttatttg gctcttgaaa aagtggtcaa aaccattcgt gaagaacgcc
    16141 gtccgttttt ggttcatgct aaagttccgt tgttaaatca ccatacatcc ggtgttcgaa
    16201 tggaatggta ccgtgatgat ttggaggaag caaagtcccg agatccttat ccggttttga
    16261 tacaacaaat gttgcaagca ggaatcacac aacaagaaat agataaacaa ctggcgttgg
    16321 ctttagctac tgtccaagaa gattttgaaa aagcccaaca agctgaagac cccaaacccg
    16381 aagatttatt cacacatgat tttgctccta caccaattat agaagaagta ggtgagcgaa
    16441 atccatcccg tgaagaaaaa gttgtgatgg tggattgtgc cttatttgcg gttgaagaac
    16501 taatgaaaaa tcatcccgaa tgtttgttgt acggacaaga tgtgggcgga cgattaggcg
    16561 gtgtttttag agaagcagca acgctagctc agaaattcgg agatgaacgc gttttcaata
    16621 cccctattca agaagcattt atcgtaggtt ccacggttgg aatgtcggcc gttggtttaa
    16681 aaccaattgt tgaggtgcaa ttcgctgatt atatttggcc gggattgaat cagttgttca
    16741 ccgaagtcag ccgttcgtgt tatttatcta acggtaaatg gccggtgagt atgattttgc
    16801 gtgtacccat tggagcatat ggtagtggcg gtccgtatca ctcttcgtca gtggaaagcg
    16861 tgctaacaaa tattcgtgga attaaaattg cgtatccaag caacggtgct gatttaaaag
    16921 ggctacttaa agcagcttat tatgatccga atccggtagt gattttggag cataaaggtt
    16981 tgtattggag caaagtgaaa ggtacggatg cggcaagagt aaacgaacca agtgaagact
    17041 atctgatacc tttcgggaaa gccaatatcg tgcaagaaat atgggaacag gaaactgccg
    17101 aaactctaac agtaattact tacggcatgg gagtacattg ggctttgaat gcttctgcta
    17161 caattaaaaa tcaagtggaa ataatcgatt taaggacttt gtttcctttg gacgaagaaa
    17221 ctgtgtttaa atctgttaaa aaggctaaga agtgcttaat tgttactgaa gagcctacaa
    17281 ataattcttt tgccagaagt ttagcgggac ttattcagga aaagtgcttc aaataccttg
    17341 acgcaccggt attggtcatt gggtcagaga atatgcctgc cataccgttg aacagcacgt
    17401 tagaattcac gatgatccca aatgttgata aggttcgtgc taagatggag gagttgcttg
    17461 agtattagtt taaagtcact acattcccgc aatcttgtca tctcgacgaa ggagacccga
    17521 acgatagcga acaggagaag cagatctcat aatttaaggt tgatattttt caatgttaac
    17581 taaataactg cttagttaaa atagtgactt atttttttaa gtagctattc cagctttctc
    17641 ccgaagcttc gggacaatct gggttagggg attgtatttc ataattaaat tcacaaaaaa
    17701 accgaagcat ctctactccg gtttatttgt ttactgcgac tgaaaatttt acttcttatc
    17761 cggtggatat tgagctaaac tcttagtcac aaattcttta ataagttctt cttttttatc
    17821 catgttttga gttaaaacac caacgccttg accttgccaa attaattctt ttttcttggc
    17881 atcgattaaa tcaatgaata gggtgccttc gacataagac gatacggatg tatttcctcc
    17941 ccataaataa ggattccagc cataacccca gccataaccc caaccggcat tgaattggtt
    18001 aacgtctact tgttcgcgtt ctttggtgaa aatgtttact aataaatctg ggttttcact
    18061 tttagtaaat cctttggcag ccatttgttc atcaatggct ctaagaattc tttttttgtc
    18121 taaatctgaa atttcaactt tgtcaatacc acttttttga aaagcatacg tcttaaattg
    18181 attgaaatca acatttttgt cataatcata atttacttta actgaactac aggaagtaaa
    18241 cagaaagagc agaagcaggg gtaggaattt gagtgttttc atagcttttt ttaattatta
    18301 aaatcaattt tcaattaaat tttttttaaa tttttataac cggtattcct tttttaacca
    18361 acaaataaaa attagttagt tgtatttaaa gtatatcttc attcactaaa ttagggatgg
    18421 taatttttaa taaaggttgc gtctccatgg ctctttttat ggcaaaaatg gctccttcat
    18481 ttcgtgccca atttctgcgg gcgattccgt tgttgacatc ccaaaacaac atcgatttta
    18541 gtcgtctttc gctatcttct gtaccatcaa gaaccatacc aaatccaccg ttaattactt
    18601 ctccccagcc aactccacca ccgttatgta ttgataccca agttgctcca cggaaactgt
    18661 cgccaatcac gttgtgaatt gccatatctg ccgtaaaact cgaaccatca taaatattgg
    18721 acgtttcgcg ataaggagaa tcggttccgg acacatcatg gtggtcacgg cctaaaataa
    18781 cataaccaat ttcttttttt gctatcgcat tattgaaagc ctctgcaatt ttcattcttc
    18841 cttctgcatc ggcatatagt attctggctt gtgaacctac cactaattta tttttttgag
    18901 cttctttaat ccatttgata ttgtcttgca tttgttgttg aatttcaatt ggagctgttt
    18961 tcgccatttc ttctaaaacg gcacaagcaa tttgatcggt tttggctaaa tcttccggtt
    19021 ttccactggc acaaacccat cggaacggac caaatccgta atcaaaacac atgggcccca
    19081 tgatatcttg tacataacta gggtatttaa aatgaatacc gtcaatggcc attacatcgg
    19141 ctccggcacg tgaggcttcg agcaaaaagg catttccgta atcaaaaaag taagttcctt
    19201 ttgcggtatg tttattgatg gctgctgcat gtcgaaccaa cgtttctttc acttttgttt
    19261 tgaacatttc cggattgatt gccattaatt catttgattc ttcaaaagat agtcctaccg
    19321 gataatatcc tccagcccat ggattatgca gggaagtttg gtcggaacct aaatcgatgt
    19381 gaatattttc tgaatcaaaa cgttcccaaa catcaacaat attcccaagg taagcgatgg
    19441 aaactacttc tttttgagct tgtgcttttc gaacacgaat cactaaatct tccacctttt
    19501 caatcacttc atttatccaa ccttgttcgt gacgaatttt agtaattttt ggatttactt
    19561 ctgcacaaat ggtgatacag cccgctatgt tgcctgcttt gggttgagca ccactcattc
    19621 caccaagtcc agatgtaaca aaaattgaac cttccggatt ttttttgatt tttcgaaagc
    19681 catttaaaac ggtaattgtt gttccatgca cgattccttg cggaccaatg tacatgtaac
    19741 ttccggctgt catttggcca tattgagaaa cccctaacgc attcattttt tcccaatcat
    19801 ctggtttgga ataatttgga attaccattc cattagttac tacaactcta ggagcctctt
    19861 tatgcgacgg aaacaatccc ataggatggc ctgaatacat cactaaggtt tgttcttcag
    19921 tcatttcaga aagatatttc attgtcaatc gatactgagc ccaattttga aaaacagcac
//
LOCUS       DAOTCW010000033        19724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1498_length_19724_cov_39.008999, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000033 DAOTCW010000000
VERSION     DAOTCW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 19724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19724
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1498_length_19724_cov_39.008999"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="P5335_04340"
     CDS             complement(<1..536)
                     /locus_tag="P5335_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRZ74141.1"
                     /translation="MGRAFEFRKARKMKRWSAMAKTFTRIGKDIVMAVKEGGPNAETN
                     SRLRAVMQNAKAANMPKDNVERAIKKASDKDTANYKEVLFEGYAPHGIAILIETATDN
                     NNRTVANIRSYFNKCNGTFGTQGSVEFMFDHTCNFRIPAEGQDVDELELEMIDFGVEE
                     IFADEDGILMYAPFESFGA"
     gene            complement(626..1708)
                     /gene="gcvT"
                     /locus_tag="P5335_04345"
     CDS             complement(626..1708)
                     /gene="gcvT"
                     /locus_tag="P5335_04345"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRZ74142.1"
                     /translation="MKNTALSHIHESLGAKMVPFAGYNMPVQYEGVTAEHETVRNGVG
                     VFDVSHMGEFLLTGPNALALIQKVTSNDAATLYNGRAQYSCLPNNDGGIVDDLIVYKI
                     KEEQYLLVVNASNIDKDWNWISSHNDLDVEMKNLSEDYSLLAIQGPKAMEAMQSLTSI
                     NLAEIKYYHFEVADFAGIEHVIISATGYTGSGGFEIYCKNSEVIQIWNKVFEAGASFG
                     IKPIGLAARDTLRLEMGFCLYGNDINDTTSPIEAGLGWITKFNKEFTNSENLKKQKEA
                     GVTKKLVGFELTERGIPRHDYEIVDADGNNIGIVTSGTMAPTVGKGIGMGYVKTEFAS
                     EGSEIFIQIRNNKVAAKVVKLPFYKK"
     gene            1924..3063
                     /locus_tag="P5335_04350"
     CDS             1924..3063
                     /locus_tag="P5335_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRZ74143.1"
                     /translation="MKQEENIVPQHIEKEIIEPQLNMLLTTDEEDERPVYIAGNFNNW
                     VTQDKNFQMEKVGKGLYHFKFPFDFIYPDEMLYKFTRGDWSEVEIDKYGNRTENRSCK
                     QHNGLKKEHVAKWRRNWLPFKPNFLPKVHLISKEFEIPQLNKTRKVWALLPHDYETST
                     EHYPVMYLQDAQNLFNEKAKYGNWEIDKKLAVMAEYKIGKIIVIAVEHADNERIIEYN
                     VGNTVLGVGQGKKYIRFLTDTLKPFIDANFRTKPEREHTGIGGSSMGGLVSIFSGIMY
                     PEVFGKLMIFSPSLWVVPKIKLSFLDMEESQDTRIYLYAGGDESATMIDHVKNFKKRL
                     HKKDAINDKMKVRLSINEQGKHNEVYWSDEFPKAIEWLFFSTKED"
     gene            3110..4519
                     /locus_tag="P5335_04355"
     CDS             3110..4519
                     /locus_tag="P5335_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HRZ74144.1"
                     /translation="MKIQYTNSQSLKKLNLIPYFETEESIVFNEIKLSKSVFSGKKDS
                     LHLFEKDENLFLFIGLGKEPDYKVIKTAFRRISAKQILHFKEEAKLFLSADFSNENVE
                     AIISGLWLGTYQLGHYKKDKKQHPLENDDFDFEINSTLEIKNTVIKSLKIAKAQLETY
                     RLVDLPPNKITPKHLADWAMQTGKNYGIEVTVIGSEQAYILGLDAFNAVGKGSANESQ
                     FIIMDYHPTTAKKHIGLVGKGITFDTGGLNIKTAGMVHMKCDMAGGAAVLGTMQLIAD
                     LQLPYRVTAIVPACENSVDANSFLPSDVIGSYSGHSIEIIDTDAEGRLILADGLAYLI
                     KNYNPETVLDIATLTGSSVATFGYECGALFSKNDMLAKQLQKAGDVTGERLWPLPLWD
                     VYKSDIESEIADVKNYSGKPVAGAISAAKFLEFFTENHVSWAHLDIAGVSFVDDEFAK
                     TKHASAFGVHLITNFIENL"
     gene            4523..5740
                     /locus_tag="P5335_04360"
     CDS             4523..5740
                     /locus_tag="P5335_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRZ74145.1"
                     /translation="MENTAKTFICISNYFKGNDFLINMKKLGNKVYLITSEKLRNKPW
                     AHDSIEEIFYMPGQDVDWDLDMLLHGVAGLMRKEKIDAIVALDDFDVEKATFLRENIR
                     MDGMGQTTGRYFRDKLAMRVKAQSEGVPVPAFSALFNDEYINHFADTVSPPWVLKPRS
                     EASATGIMKVYSKEELWQKIHELGDNRIKYLVEQFKPGAVYHCDGLNFNSKVLFSLTS
                     QYLATPMEISQGGGIFRSANIKYNSADDRAIKKVNEQVMKSFGMKHGATHTEFIKCNE
                     DGKIYFLETSSRVGGAHLAEMVEAASGINLWGEWAKIEDALVRGIEYKVPSPKNQFAG
                     IVLTLSKYEYPDLSGFNDIEICFRVPLEYHAGLIVKSDKPERVRELLDDYAERLVQDF
                     STVVKQKEVKKFH"
     gene            5844..6386
                     /locus_tag="P5335_04365"
     CDS             5844..6386
                     /locus_tag="P5335_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74146.1"
                     /translation="MKKYNSKNFHKHTFCLWKEVSQTDFELQKSHFKSKSGSEYFFTE
                     EGVYRISNHWGRAANCRWRLISLENYKNQETKVGFAKWIDFYPNDETTNLFFIQVDFE
                     MKTVDFFHKEIPFYDNKATLRNASETTKTIKIIKQVLNEEDWAKYLEYEDLEGLREDF
                     VMKMVYDGMSFGEARRNLIK"
     gene            6575..6880
                     /locus_tag="P5335_04370"
     CDS             6575..6880
                     /locus_tag="P5335_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74147.1"
                     /translation="MAKVNGLINIDKNLSNCTLFIKNENHIVKIPKIDYLFDVNFIEV
                     NFYDSENKQLFEKKYNKEFKRKGKSYQVYFGLEDIYFLDYSKKQIRKLNINFRNLKY"
     gene            complement(6977..8167)
                     /locus_tag="P5335_04375"
     CDS             complement(6977..8167)
                     /locus_tag="P5335_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74148.1"
                     /translation="MNPLYRKQFNEQFTFEKYNHLIESISADFDYKPTFRIGETPFFI
                     SNELKNQLLEGCHEVIHFIQKNDFKDLTSKALDLNRKVPNEDNHTTFLAIDFGICEEE
                     GKIIPKLIEVQGFPSLYNFQSNLAHKFEGLYPFLSELTPYLNGLDNESYLKLIEEVIC
                     NNYPKENVILLEIEPEKQNTKIDFYYCKRDIGIPIVCVTELIQKGRKLYYTNDKGLEV
                     EVKRIYNRVIFDELDLRSDLKLNFDFSADLEVEWAGHPNWFFRISKFILPYLKGKYFI
                     ETTLLSDLKVIPTDLENYVLKPLFSFSGSGVIFHVKKEDIEAVKEKDLYILQKKVDYL
                     PIIQSPDGKVKAEVRLLAVWKKDAPSPTLVTNLVRLSRGEMIGVKFNKDKDWVGGTVG
                     MFEK"
     gene            complement(8242..9069)
                     /locus_tag="P5335_04380"
     CDS             complement(8242..9069)
                     /locus_tag="P5335_04380"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HRZ74149.1"
                     /translation="MNRQIRIAVLDMYDGEPNQGMRCIFDIINGFNQMVSFQVFDIRG
                     KKEFPDIKKFDIYISTGGPGNPLEGDGDWDVKYFELIDSIWKWNKENDMKKHVLFICH
                     SFQMACKHFGLAEVNKRKDTSFGVMTTHKVNEGMNDPLFEGLTNPFYTIDSRDFQVVQ
                     PHLKNFKKMGAQIIALEKIRDHVQYERAVMGIRFSNEFVGLQFHPEADPLSFLAHMKD
                     KNFREKIIKMKGRVKFLKMLENLVEEDKIYQTNETIIPNFLRIAINDLLKHKKILSN"
     gene            complement(9221..10330)
                     /locus_tag="P5335_04385"
     CDS             complement(9221..10330)
                     /locus_tag="P5335_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HRZ74150.1"
                     /translation="MKKELPVFTLGVEEEYLIIDPDTRDLRSHMSKIVDGAKIILQEQ
                     VKAEMHQSVVEVGTNICKNVKEAREEITFLRNKIVELAANQGLVVGAAGTHPFSKWQD
                     QPITDDPRYHTIVNELQDAARSNLIFGMHCHVGIESREIGLQLINQACYFLPHIFALS
                     TNSPFWEGRNTGYKSFRTKVFDKFPRTGLPEYFDSVQSYDNFLETLVKTNCIDNPKKI
                     WWDLRLHPFYNTIEFRICDMSLTVDETICIVAIIQAIVAKLYRLNMANTSFNVYRLAL
                     IKENKFRAARYGVQGNMIDFGLQKEVPTKELILELLEFIDDVVDELGSRDEINYVHTI
                     LREGTGAAKQLAVFEETQDLTKVVDFITSQFTKGI"
     gene            complement(10382..>11254)
                     /locus_tag="P5335_04390"
     CDS             complement(10382..>11254)
                     /locus_tag="P5335_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74151.1"
                     /translation="GIIAEAVSIDKVVQNKATEYAVIIDRISQDVPFYRAYLKNAALT
                     GTAVINNPFWWSADDKFFNNCLADVLGVPLPNTVILPSAEHPTDTTSKSFRNLKYPMD
                     WEAIFDYVKFPAYMKPYAGGGWKNVYRLEDRDMFWEIHRETGQLVMLLQEEIVFDQYF
                     RVYCLGGKAVRIMPYEPRNPHHLRYVVDGPPADKKLLATVKDYTLRLCKGLGYDFNTV
                     EFAVRDGIPYAIDFGNPAPDAELTSVGKENFEWVVEEAAKMAIEYAKKHKDGQMNLTW
                     GTFMKGSVNDAKRF"
     assembly_gap    11255..11354
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11355..12057)
                     /locus_tag="P5335_04395"
     CDS             complement(<11355..12057)
                     /locus_tag="P5335_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRZ74152.1"
                     /translation="MKEEYFKWHSPNLGREIQMLVFGHAGYPVILFPTSMGSFYENRD
                     QGLIESAKWYIEQGLIQIFCPDSIDKDSFYNKNIHPTHRIQNHVWYDKMICNEIVERV
                     KNNTYSGKVAVAGCSFGGYHAANFAFRHPGYVSHVFSMGGAFNIKNFMDGYHDDNVFY
                     NSPEDYLHGLNDRELWNMDIILGTSNWDICFDANLKLSRVLAHRDVPHWLDIRQNREH
                     DWPVWKEMFPHYLSRI"
     gene            12416..13153
                     /locus_tag="P5335_04400"
     CDS             12416..13153
                     /locus_tag="P5335_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRZ74153.1"
                     /translation="MPLIFSCSPKILKSESVSEATLIKSKNTGYFHAIGTEPFWNLEI
                     SENSIRFKEVTADYEINISHVEPVKVMDANIKMYISVSEEQEMKIIIEQKDCSDKMSN
                     NKYSYQVKVELKKKNQTAITTFYGCGNYIADYRLYDNWVLEELKGKKVSKDNFANELP
                     LLEINASDKKIMGYAGCNRINGTLFQERELLRFTNIISTRMACNQANLEQEYLKCLQS
                     STQYEIKNNRLYLSNPSELLIVFKKID"
     gene            complement(13150..13554)
                     /locus_tag="P5335_04405"
     CDS             complement(13150..13554)
                     /locus_tag="P5335_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HRZ74154.1"
                     /translation="MNNRDFYIIENRGEALGVVNEQSSKEEKFQNLTIRPILKLQNDL
                     FIDVFKNYVSKHKNDFYSYSVEKKLLFIENAIQKDIKFRNSLKGMVIGLFTIDEYAEY
                     IQNSSNLNKRMMNMLIERLKSNVQIFEFQDLS"
     gene            complement(13633..14934)
                     /locus_tag="P5335_04410"
     CDS             complement(13633..14934)
                     /locus_tag="P5335_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRZ74155.1"
                     /translation="MKKLILLISFSVLTLTQSFAQDKDTLKTDLNDEYNKWSFEFNLG
                     QSKGIKPYSIGYFGSDPTKSFGSVTLNHYSGGVRYMFSPKFGLKADVAFDRLENLSDE
                     SLDFEMQHIRFGVQGVVNAARLFDIQEPLGRFSFLFHGGIQVAQMTPQMGINKDRDEW
                     NGGIMFGFTPEFRITKHLAMNADFTMNSYVRQHFNWDGAYSDPNNNLAGSLYTISLGA
                     SFSFGAEKIHGDWAVLRDKQQEEIEALEKRVGEIENNMGDTDQDGVPDYLDVEPNSIA
                     GIAVDTKGRMVDLNKNGVPDEMERYLNSTYTTNQMLVNSEKETVKKLINEGYVATYFD
                     FNKTKPTNVSTEGIDFILTYLRNNPTATVDIIGHADEIGKNPYNDKLAATRANSVKEI
                     LLKAGITEDRLNVVSEGEDTSVDKDSAGARRLVRRVTFKVK"
     gene            15221..15610
                     /locus_tag="P5335_04415"
     CDS             15221..15610
                     /locus_tag="P5335_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRZ74156.1"
                     /translation="MRFHTRKWVKPEDLNPNGTLFGGRLLAWIDEEVALYSIIQLENP
                     RVVTKHMSEINFKSSAKTGDIIEIGIDVVKFGKTSITLSCQVRNIMTREIIISVDTIT
                     MVNLDQEGKPLVHGKTKIEFITERLDK"
     gene            complement(15611..15889)
                     /locus_tag="P5335_04420"
     CDS             complement(15611..15889)
                     /locus_tag="P5335_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HRZ74157.1"
                     /translation="MLEIFRKTAWFEGLSLILLLFFAMPMKYLAGQDIFVKTIGMAHG
                     LLFILYILLALTLKFSSPWNYKKFGIICLASIIPFGTFYIESKYINKD"
     gene            complement(15891..16502)
                     /locus_tag="P5335_04425"
     CDS             complement(15891..16502)
                     /locus_tag="P5335_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019038699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HRZ74158.1"
                     /translation="MQRERIKKNKWKQLHLYYTYTGFYKFIFEGIKSAFLPTLLIFGA
                     LFYVHYYVININEALIMLTERYSDFAIFSVFFISESILGLLPPDIFIAWTKNTDFPLI
                     YLSLLAILSYLGGVVSYITGKAILYIPSVNKFMSEKMTKHIINMRKWGGLLIAVGALL
                     PLPFAMASMAAGMIKFNFKNFLLFALLRLMRFVIYGFAIYQML"
     gene            complement(16505..17866)
                     /gene="nhaA"
                     /locus_tag="P5335_04430"
     CDS             complement(16505..17866)
                     /gene="nhaA"
                     /locus_tag="P5335_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRZ74159.1"
                     /translation="MKYRLKITPIDKWVINPVNRFIGKSTTGGIVLFLAAVVALILAN
                     SPWKEAYHHFWEHKFSLGFDNKYLLDQDLHHWINDGLMAVFFFVVGLELKREIIGGEL
                     SNPKKAFLPVAAGFGGMIFPALIYLLFNSTGEAQSGWGIPMATDIAFALGVLFLLGDK
                     VPTSLKLFLTVLAIADDIGAVLVIAFFYTSDISFTSLIIGTAFLLLLIISNLVGVRNT
                     IYYGILGIGGLWMAFLLSGVHATIAGVLAAFTIPANVKIDEIGFSEKMKMYLEKFKKA
                     DPNGVPTVTPEQLHILEDMRTLSKQAMTPLQRLEHGLHPIVTFIIMPIFALANAGVTL
                     SSETATTTSSYVAIGVIFGLLIGKFIGIVGVTSLFLKLKLASLPEGMNYKHLIGVGLL
                     ASIGFTMSLFVANLAFDNNELIQQAKIGILIASLTGGTIGFLYLYFIKPKATIIIKES
                     DIH"
     gene            18060..19598
                     /locus_tag="P5335_04435"
     CDS             18060..19598
                     /locus_tag="P5335_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRZ74160.1"
                     /translation="MSKKDILLEYEEKKKLYSYQLGDIDLIFDKIETAPHNYHLLYQL
                     PTGGGKTVIFSEITRRYLEKYDKKVVVLTHRIELCKQTSKMLKGFDVKNKIINSVVKD
                     LPDQNDYSCFVAMVETLKNRLNDEKLHIDNVGLLIIDEAHFNSFRKLFHAFKNTFFLG
                     VTATPLSSNMKLPMGENYHELVVGHSIASLIDKGYLAKATTYSYDVGLTSLKIGINGD
                     YTVKSSDELYTNLAMQEKLIHAYSAHSMGKKTLIFNNGIITSLSVLDTFREAGFKNIK
                     HLDNTNSNEERKDILKWFKDTPDAILTSVGILTTGFDEPTVESIILNRATKSLTLYFQ
                     MIGRGSRKLPNKDAFSIIDLGNNAARFGLWTDKLDWQHIFKYPEQYIHNLKGDVEIEM
                     NFYYTMPEELKAKFRNTPDITFDVETEYRKALSKNLRPKVVIEKSIEHHASMCVDNSE
                     TLVEAKQLAKELAEDIESRVRHFTNCLSSSTKNYREWLIEDYKTRLTVTIGRKFREKI
                     FSMD"
BASE COUNT         6729 a         3141 c         3191 g         6563 t
ORIGIN      
        1 gctccaaaac tttcaaaggg agcatacatt aagattccat cttcgtcagc aaaaatttct
       61 tctacgccaa aatcaatcat ttctaattct aattcatcca catcttggcc ttcagcaggt
      121 attctgaaat tacaggtgtg atcaaacata aattcaaccg aaccttgtgt accaaaagta
      181 ccattacatt tgttgaaata acttctgata ttggcaactg ttctgttatt attatcggtg
      241 gccgtttcaa tcaaaatagc aatcccatga ggagcatatc cttcaaatag aacttcttta
      301 tagttagcgg tatctttatc cgatgctttt ttaatagccc tttccacatt gtctttcggc
      361 atgttagccg ctttggcatt ctgcattacc gctcttaatc tggaatttgt ttctgcattc
      421 ggaccgcctt ctttcacggc catgacaatg tcttttccta ttctggtaaa cgttttagcc
      481 atcgcagacc aacgtttcat tttacgtgct tttctaaatt caaacgctct tcccatttct
      541 gtttatttca atattctaat ttagttgttc aatgttcaaa atttgactgc aaaaataaca
      601 cttaaactgt tatttcaaaa aattattact ttttataaaa aggcaatttt actacttttg
      661 cggctacttt attatttctg atttgaataa aaatttcact tccttcagag gcaaattcag
      721 ttttcacata gcccattcct attccttttc caacggttgg agccatagtt ccggaagtca
      781 caattccgat attatttcca tctgcatcta caatttcata atcatgcctt ggaattcctc
      841 gttcggttaa ttcaaaacca actaattttt tggtgacacc ggcttctttt tgttttttca
      901 ggttttcaga attagtgaac tctttgttga atttggtaat ccatcctaaa ccggcttcaa
      961 taggagaagt ggtatcatta atgtcgtttc catataaaca aaaacccatt tccaatcgca
     1021 aagtatcacg agcagctaat ccgataggtt tgataccaaa agaagctccg gcttcaaaaa
     1081 ctttattcca tatttgtatc acttccgaat ttttacaata aatttcaaat ccacctgacc
     1141 cggtataacc ggttgcggaa atgattacat gttcgattcc ggcaaaatca gccacttcaa
     1201 aatgatagta tttaatttca gctagattaa tggatgtcaa cgattgcatg gcttccattg
     1261 cttttggtcc ttgaatggct aataaggaat aatcttcaga aagatttttc atttcaacgt
     1321 ctaaatcatt gtgagacgaa atccaattcc aatctttatc gatattggat gcattaacga
     1381 ctaataaata ttgttcctct ttaattttgt aaacgattaa atcatctaca attccaccat
     1441 cattatttgg aaggcaagaa tattgtgctc taccattata taatgtggct gcatcattag
     1501 aagttacttt ttgaattaga gctagtgcat tagggcctgt taacaaaaat tcccccatgt
     1561 gcgatacatc aaaaacgccc accccgtttc gaaccgtttc atgttcggca gtaactcctt
     1621 catattgaac aggcatattg tatcctgcaa atggaaccat tttagctcct aaactctcgt
     1681 gaatgtgtga taaagctgta tttttcatta gtttgttgtg tataatttga agtggctaat
     1741 ttattaaaaa taatgaaatt tttgagtgaa atacgctgtc aaatttattt aattgttact
     1801 tttttattcg attagtcatt attcttgcat aaatcagatt tctatccaaa aatattttca
     1861 ttcacaatcc taaaaacata tatttacttt tttaataatt aatgcattcc attttttgat
     1921 agcatgaagc aagaagaaaa tatagtacca caacacattg aaaaagaaat tattgaaccc
     1981 caactcaata tgcttttaac tactgatgaa gaggatgaaa gaccggttta tatcgcagga
     2041 aattttaaca attgggttac acaggataag aattttcaaa tggaaaaagt aggaaaggga
     2101 ctttatcatt ttaaatttcc ttttgatttt atatatccgg acgaaatgtt atacaaattc
     2161 acccgaggtg attggagtga agtagaaatt gacaaatatg gaaatcggac tgaaaatcgt
     2221 tcgtgcaaac aacataacgg tctaaaaaaa gagcatgttg ccaaatggag aagaaattgg
     2281 ttgcctttta aacctaattt tttacccaag gttcatttaa tttctaaaga atttgagatt
     2341 ccacagttga ataaaaccag aaaagtgtgg gcattgttgc cacatgatta tgaaacttcc
     2401 acagaacatt atccggtaat gtatttacag gatgctcaaa atttattcaa cgaaaaagct
     2461 aaatacggaa attgggaaat tgataaaaaa ttggccgtta tggccgaata taaaattggt
     2521 aaaatcattg taattgccgt tgagcatgct gataatgaac gaattattga atataatgtg
     2581 ggtaataccg tattaggtgt cggacaagga aaaaaataca ttcggttttt aactgatacc
     2641 ttaaaaccat ttatagatgc caatttcaga actaaaccgg aacgtgagca taccggtatc
     2701 ggcgggagtt ctatgggtgg attggtgagt atttttagcg ggattatgta tcctgaagta
     2761 tttggaaaac tgatgatttt ttcgccctcg ttgtgggtag tccctaaaat taagttgtct
     2821 tttttagata tggaagagtc gcaagacacc cgcatttatt tgtatgccgg aggagatgaa
     2881 agtgcgacaa tgattgatca tgtaaagaat tttaaaaaaa gactgcataa aaaagatgcc
     2941 attaatgaca aaatgaaagt acgattgagc atcaacgaac aaggaaaaca taacgaagtg
     3001 tattggagtg atgaatttcc aaaagcgata gagtggttgt tttttagtac aaaagaggat
     3061 tgacaatatt cctttcaatt tcaaaaatta aataaaaaat tgtttcttta tgaaaattca
     3121 atatacaaat agtcaatcct taaaaaaact gaacttaatc ccgtattttg aaacggagga
     3181 aagtattgtt tttaatgaaa ttaaactttc aaagtcagtt ttctccggaa aaaaagacag
     3241 tcttcacctt tttgaaaagg acgaaaacct ttttctattc attggattag gaaaggaacc
     3301 cgactataaa gttattaaaa ctgcgtttag aagaatctca gccaagcaaa ttttgcattt
     3361 taaagaggag gctaagcttt ttctttccgc tgatttctcc aacgaaaatg tggaagcaat
     3421 tatttcagga ctttggttag gaacctatca attaggccat tataaaaaag ataaaaagca
     3481 gcatcctttg gaaaacgatg attttgattt cgaaatcaat tcaacattag agattaaaaa
     3541 tactgttatt aaaagtttaa aaattgccaa agctcaatta gaaacatatc gtttagttga
     3601 tttacccccc aataaaatta cccccaaaca tttagccgat tgggctatgc aaacgggtaa
     3661 aaattatggt attgaggtta cagtaattgg ttcggaacaa gcctatattt taggtttaga
     3721 tgcctttaat gcggtaggca aaggaagtgc caacgaatca cagtttatca tcatggatta
     3781 tcatccaacg actgctaaaa agcacatagg cttggttggt aaaggaataa cttttgatac
     3841 cggtggttta aatataaaaa cggcaggaat ggtccatatg aaatgtgata tggctggtgg
     3901 tgcagccgtt ttgggaacca tgcaactgat tgccgattta caattgcctt atcgggttac
     3961 tgctatcgtt cccgcttgtg aaaattcggt agatgctaat tcttttttac ccagcgatgt
     4021 aattggaagt tacagtggtc attccattga aatcattgat accgatgctg aaggaagatt
     4081 aattttagcg gatggattag catatttaat caaaaattac aacccggaaa cggttcttga
     4141 tatcgctacg ctaaccggaa gcagtgtcgc cacttttgga tatgaatgtg gagcgttatt
     4201 cagtaagaat gatatgttag cgaaacagtt gcaaaaggca ggtgatgtaa cgggtgaacg
     4261 actttggcct ttgccgttat gggatgttta taaatcggat attgaaagtg aaattgccga
     4321 tgtaaaaaat tattcaggaa aaccggtcgc cggtgctatt tctgctgcaa agtttttaga
     4381 attttttact gaaaatcatg tttcatgggc acatctcgat attgccggcg tttcatttgt
     4441 agatgatgaa tttgccaaaa ccaagcatgc ttctgcattt ggtgtgcatt taattactaa
     4501 ttttattgaa aacctatagt ctatggaaaa tactgctaaa acattcattt gtatttctaa
     4561 ttattttaaa gggaatgatt ttttaatcaa tatgaagaaa ttgggtaaca aagtttatct
     4621 cataacttca gaaaaattac ggaacaaacc ttgggctcat gattcaattg aagaaatttt
     4681 ttatatgccg ggacaggatg tggattggga tttagatatg ctattgcatg gggtagcagg
     4741 attaatgcgg aaggaaaaaa ttgatgctat tgtagcctta gatgattttg atgtggaaaa
     4801 agcaactttt ttacgtgaaa atatccgaat ggatggaatg ggacaaacga ccggtcgtta
     4861 tttcagggat aaattagcca tgagggtaaa agcacaatcg gaaggcgttc cggttcctgc
     4921 ttttagtgcg ttgttcaatg atgaatatat caatcatttt gccgataccg tttcgcctcc
     4981 atgggtttta aagccacgtt cagaagcttc agcaaccgga attatgaaag tgtattcgaa
     5041 agaagaatta tggcaaaaaa ttcatgaatt gggagacaat cgtattaagt atttggttga
     5101 acagtttaaa cccggagcag tatatcattg tgacggattg aattttaact caaaagtgtt
     5161 gtttagtttg acttcgcaat atttagccac gccaatggaa atttcgcaag ggggtgggat
     5221 ttttagaagt gcaaacatca agtacaattc cgccgatgat agagccatta agaaagtaaa
     5281 tgagcaagtc atgaaatcat ttggaatgaa acacggagcc acacataccg agtttattaa
     5341 atgtaatgaa gacggaaaaa tttacttttt agaaacgtct tcccgagtag gaggtgctca
     5401 tttagccgaa atggttgaag ccgcatccgg tatcaattta tggggagaat gggctaaaat
     5461 tgaagatgct cttgttcgtg gaattgagta caaagtacct tctccaaaga atcagtttgc
     5521 tggaattgta ttgaccttat ccaaatatga ataccctgat ttgagtgggt ttaatgacat
     5581 tgaaatttgt tttcgggttc cgttagaata ccatgccgga ttgattgtaa aatcagataa
     5641 accggaacgt gttcgtgaat tgttagatga ttatgcagaa cgactggtgc aagatttttc
     5701 tactgttgtg aaacaaaaag aagtaaagaa atttcattaa attaaaacat agtaattttt
     5761 aatgttttat caattaagtc atataaggaa tatatttttt taaagcttat gtgacttatg
     5821 tttttaatat tcataattta aaattgaaaa aatataactc caaaaacttc cacaaacaca
     5881 ctttttgcct ttggaaagaa gtttctcaaa ctgattttga gttacaaaaa tcgcatttca
     5941 aaagtaaatc ggggagtgaa tattttttta ccgaggaggg agtttaccgt atttccaatc
     6001 attggggtcg agcggccaat tgccgttggc gattaatttc tttagaaaat tataaaaatc
     6061 aagagacaaa agtgggtttt gcaaaatgga ttgattttta cccaaatgat gaaacgacca
     6121 acttattttt tattcaggtt gattttgaaa tgaaaacggt tgattttttt cataaagaaa
     6181 taccatttta tgataataaa gcaactttac gaaatgcctc tgaaaccact aaaacaatca
     6241 agattatcaa acaagtttta aacgaagagg attgggcgaa gtatttggag tatgaagatt
     6301 tggagggttt acgagaagat tttgtaatga aaatggttta tgatgggatg agttttggag
     6361 aggcgaggag gaatttgatt aaatagttta aatttaagaa acattatgaa actatgttaa
     6421 aaaaaatatt atttttagtt ttattttagc ttcaacttca atttttgctc aagaaattat
     6481 ttctagaaat atagaaattt tattttttga aaatgaaaaa agagttgaat atgaagttta
     6541 tgctgttaat gataatacaa atttaatctt aaaattggca aaagttaacg gtcttattaa
     6601 tattgataag aatttaagta actgtacatt attcattaaa aatgaaaatc atattgtaaa
     6661 aattcctaaa atcgattatt tgtttgatgt aaattttata gaagtaaatt tttatgattc
     6721 tgaaaacaaa caattatttg agaaaaaata caacaaagag tttaaaagaa aagggaaaag
     6781 ttatcaggta tattttggtt tagaagatat ttattttcta gattattcaa aaaaacaaat
     6841 aaggaagtta aatataaatt ttagaaacct taagtattga taatattgat tatttgtgtg
     6901 agacgaatat aatatttcta ttcaagtaaa ttatcataac gatttgcaaa ctttcctaac
     6961 attttacctt caactactat ttctcaaaca tcccgactgt accaccaacc caatctttgt
     7021 ctttattgaa ttttacacca atcatttcac ctcggcttag tcggactaaa ttggtcacta
     7081 gggtaggcga gggagcgtct tttttccaaa cggctagcaa gcgaacttcc gcttttactt
     7141 tgccatccgg cgattgaatg atgggtaggt agtctacttt tttctgtaga atgtataagt
     7201 ctttctcttt tacggcttcg atgtcttctt ttttcacatg aaaaattacg ccacttcctg
     7261 agaaagaaaa taagggtttg agtacataat tttctaaatc ggttggaatt acttttaagt
     7321 cagataacaa ggtagtttca atgaagtatt ttccttttaa ataaggcaaa ataaatttac
     7381 tgatgcgaaa aaaccaattc ggatgtcctg cccattccac ctctaaatcg gcagaaaaat
     7441 caaagttgag tttcaaatca gaacgcaaat ccaattcgtc aaaaataaca cgattataaa
     7501 tgcgtttgac ttcaacttca agtcctttat cattagtgta atatagtttt cttccttttt
     7561 gaattaattc cgttacacaa actatcggaa ttccaatgtc gcgtttgcaa tagtaaaaat
     7621 caattttggt gttttgtttt tccggttcaa tttctaacag aattacattt tcttttggat
     7681 aattattaca aataacttct tctattaatt ttaaatacga ttcgttatcc aaaccattta
     7741 gataaggagt taattccgat agaaaagggt aaagcccttc aaatttatgg gcaagattac
     7801 tttggaaatt atataatgaa ggaaaacctt ggacttcaat tagttttgga ataatttttc
     7861 cttcttcttc gcaaatacca aaatcgatgg ctaaaaatgt ggtgtgatta tcttcatttg
     7921 gaacttttct atttaaatct aacgctttgc tggttaaatc tttaaaatca ttcttttgaa
     7981 taaaatgaat gacttcatgg caaccttcta acagttgatt ttttaattca ttggatataa
     8041 aaaaaggtgt ttcaccaatt cgaaaggtag gtttgtaatc aaaatcagcc gagatacttt
     8101 ctattaaatg attgtatttt tcgaaagtaa attgttcgtt gaattgtttt ctgtataatg
     8161 gattcataac ctgctttttt aaccaaaaag gcacaaaaga attaaactga tgtgccttta
     8221 tagaaattac tattattcaa tttaattgga aagaattttt ttatgcttta ataaatcgtt
     8281 gatggctatt cgaaggaaat ttggaattat cgtttcatta gtttgataaa ttttatcttc
     8341 ttctaccaga ttttccaaca tttttaagaa tttcacacga cctttcattt ttataatttt
     8401 ttcacgaaag tttttatctt tcatgtgagc taaaaagctt agtggatcag cttccggatg
     8461 aaattgcaaa ccaacaaatt cattagaaaa tcgaattccc atgacggctc tttcgtattg
     8521 tacatggtct cttatctttt ctaaagcgat gatttgagct cccatttttt taaaattttt
     8581 caaatgaggc tgtaccactt gaaaatctct actatctatt gtgtaaaacg gattcgtcaa
     8641 cccttcaaat aacgggtcat tcatgccttc gtttacttta tgagttgtca taacgccaaa
     8701 agaagtgtct tttcttttat ttacctcggc taaaccaaaa tgtttgcagg ccatttgaaa
     8761 ggaatgacaa atgaataata catgtttttt catgtcgttt tccttgttcc atttccaaat
     8821 tgaatcaatc aattcaaaat attttacatc ccaatcacca tcaccttcta atggatttcc
     8881 tggtccacca gtggatatat aaatatcaaa ttttttaata tccggaaatt ctttttttcc
     8941 acgaatatcg aaaacctgaa aagaaaccat ttgattaaat ccattgataa tgtcaaaaat
     9001 acaacgcatg ccttgattag gctctccatc atacatgtct aatactgcaa ttctgatttg
     9061 tctattcatt tgggaataat ttaatgaagc aaatataact tatttgaatg aatgtcaact
     9121 ttgttgcttt tattgtttat taaatattct atcaacattt tgatttcttt ctaagaggtt
     9181 aatttcaact ttatcattgt tataaaggat aaaaattatt ttaaattccc tttgtaaatt
     9241 gacttgtaat aaaatcgacc actttggtta aatcttgagt ttcttcaaaa acagccaatt
     9301 gtttagcggc accggttcct tcgcgtagga tggtgtgcac ataattgatt tcgtcacggc
     9361 ttcccaattc atccaccaca tcatcaataa attctagtaa ttccaaaatt aactcttttg
     9421 ttggcacttc tttttgaagt ccaaaatcaa tcatgttacc ttgaacacca taacgggccg
     9481 cacggaattt gttctctttt atcaacgcca aacgatacac attaaaacta gtattagcca
     9541 tgtttaatct atataatttt gccacaatcg cttgaataat agctacaata caaatcgttt
     9601 catccaccgt taaactcata tcacaaatac gaaattctat cgtattataa aatggatgta
     9661 atcgtaaatc ccaccagatt ttcttaggat tatctataca atttgtttta actaaggttt
     9721 ctagaaaatt atcatatgac tgaaccgaat caaaatattc cggtaaaccg gttcttggaa
     9781 atttatcaaa aacttttgtt ctgaatgatt tgtatccagt gtttctgcct tcccaaaaag
     9841 gagaatttgt tgataatgca aaaatatgag gcagaaaata acaagcttgg ttgattagtt
     9901 gcaaaccaat ttctctactt tcaatgccga cgtgacaatg cattccaaat attaagtttg
     9961 aacgagcagc gtcttgtaac tcgtttacaa tagtgtgata ccttggatca tctgtaattg
    10021 gttggtcttg ccatttagaa aaagggtggg ttccggctgc accaacaact aatccttgat
    10081 tagcagctaa ttctacgatt ttatttcgta aaaaagtgat ttcttcccga gcctctttta
    10141 catttttaca gatattagta cctacctcca ccacggattg atgcatttct gctttcactt
    10201 gttcctgtaa aatgattttt gcaccatcta caattttcga catgtgtgaa cgtaaatccc
    10261 ttgtatccgg atcaataatc aaatattctt cttctacacc aagtgtaaat accggcaatt
    10321 ctttcttcat aatcatttta ttaaaaaaac tgttacttat ataaaaaaaa ggctatttgc
    10381 attaaaatcg tttagcatca tttacagagc ctttcataaa agttccccaa gttaggttca
    10441 tttgtccgtc tttgtgcttt ttagcatatt ctatggccat tttagcagct tcttcaacaa
    10501 cccattcaaa gttttcttta cccacagaag tcaattcagc atcaggagct ggatttccaa
    10561 aatcaatagc gtaaggaatt ccgtcacgaa cggcaaattc tacagtatta aaatcgtagc
    10621 ccaatccctt acaaagtcga agtgtgtaat cttttacggt agccaatagt tttttatctg
    10681 ccggaggacc atctacaaca tatctcaaat gatggggatt acggggttca tagggcataa
    10741 ttcgaaccgc tttaccacct aaacaataaa cacggaaata ttgatcaaag acaatttctt
    10801 cctgcaaaag cataactaat tgtccggttt cacgatgaat ttcccaaaac atatcacgat
    10861 cttctaatcg atagacgttt ttccatccac caccggcata cggtttcatg taagcaggaa
    10921 attttacata gtcaaaaatc gcttcccaat ccattgggta ttttaaattt ctaaatgatt
    10981 tgctggttgt atccgttgga tgttccgcag agggaagaat tactgtattt ggtaatggaa
    11041 cgcccaatac atcagctaaa caattattaa aaaatttatc atcggcactc caccaaaatg
    11101 gattattgat tactgcggtt cctgttaaag cagcattttt taaataggca cgataaaatg
    11161 gaacatcttg agagattcta tcaattatca cggcatattc tgttgcttta ttttgaacta
    11221 ctttatcaat agaaacggct tcagcaatga ttccnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnntaattc tggataaata atgaggaaac atttctttcc aaaccggcca
    11401 atcatgttcg cggttttgac gaatatcaag ccaatgagga acatcacggt gagccaaaac
    11461 acggcttaat ttgagatttg catcaaaaca aatatcccag ttcgaagtac ccaaaataat
    11521 atccatattc cataattccc gatcgttcaa accatgcaga taatcttcgg gactgttata
    11581 aaaaacattg tcatcatggt atccatccat aaaattttta atattaaagg ctcctcccat
    11641 tgaaaagaca tgacttacgt aacccggatg gcgaaatgca aaatttgccg catgatatcc
    11701 tccaaaacta catccggcga cagctacttt accggaatag gtattgtttt tgactctttc
    11761 tacaatttca ttacaaatca ttttatcata ccaaacatgg ttttgaatgc gatgtgtggg
    11821 atgaatattt ttgttgtaaa aactatcttt atcaatgctg tccggacaaa atatttgaat
    11881 taatccttgt tcgatatacc atttcgcgga ttcaatcaat ccttggtctc tattttcata
    11941 aaaacttccc atcgaagttg gaaataaaat aaccggatat cctgcatgac caaaaactaa
    12001 catttgaatc tctcttccta agttaggtga gtgccatttg aaatattctt ctttcatatc
    12061 attggtttta acaataatac tgaattttag gcaaaataaa acaagaggac ttgagatttt
    12121 gttattttta tgattatatg tttatgaata tgaaatgaat aaataaaata caaaaaatgc
    12181 ccacaaaatc gtactacctt tcaataattt gcaataaaaa tgatgctttt taaattgatt
    12241 acgagttgtt ttaagtgccg attatacctt tttaattaaa aaatcattta agaatataca
    12301 atttgggcag taaaatgaag taaaaaatgc gtaaatttat agaacataaa atgaaattta
    12361 tcgatttatt aaaatcaaaa ggattatgaa aaatgtgacc tacatatatc tagttatgcc
    12421 cttaattttt tcttgtagcc cgaaaattct aaagagtgag agtgttagtg aagctacatt
    12481 aataaaatca aaaaatacgg gttattttca cgcaattggt acggagcctt tttggaattt
    12541 agaaatatcc gaaaacagta ttcgatttaa agaagtaact gctgattacg aaatcaatat
    12601 ttctcatgtg gaacctgtta aagtgatgga tgcaaatata aagatgtata tatccgtttc
    12661 agaggaacaa gaaatgaaaa taataataga acaaaaagat tgctctgata agatgtcgaa
    12721 caataaatac tcctatcagg ttaaagtaga attaaagaaa aaaaatcaaa ctgcgattac
    12781 tactttttat ggttgtggaa attatattgc ggattacaga ttatatgata attgggttct
    12841 tgaagaatta aaaggaaaaa aagtgagtaa agataatttt gcaaatgaac ttccgctttt
    12901 agaaatcaat gcctctgata aaaaaatcat gggatatgcc ggttgtaatc gaatcaatgg
    12961 aactttattt caagagcgtg aattacttcg atttacaaat attatcagta ctagaatggc
    13021 ttgtaatcaa gctaacttag aacaggaata cttgaaatgt ttgcaaagtt ctactcaata
    13081 tgaaataaaa aataacagac tttatttatc taacccatcc gaattgctaa ttgtatttaa
    13141 gaaaatagat taggataaat cttgaaattc aaaaatttgc acattgcttt tcaatcgttc
    13201 gattaacata ttcatcattc ttttgttgag attggaggaa ttttgaatat attctgcata
    13261 ttcatctatt gtaaaaaggc caatgaccat accttttaga gagtttctga atttgatgtc
    13321 tttttgaata gcattttcta tgaatagcaa tttcttctca acagaataag aatagaaatc
    13381 gtttttatgt ttagaaacgt aatttttgaa tacatcaata aacaaatcat tctgtaattt
    13441 taggataggg cggatggtta aattttgaaa tttttcctct tttgaggatt gttcgttaac
    13501 cacaccaagt gcttcgcctc tgttttcaat aatataaaaa tcgcgattgt tcattttttt
    13561 cttttaaaat tataaaaaaa cctctgtcgt taaacagagg ttcatttata gttattaaga
    13621 atgaattgtt aattatttta ctttaaaagt tactcttctt accaatcttc tagcaccggc
    13681 tgaatcttta tcaacagaag tatcttcacc ttcagaaaca acattcaatc tgtcttcagt
    13741 aatacctgct ttcaataaaa tttctttaac gctgtttgct cttgtggcag ctaacttatc
    13801 attgtaagga tttttaccaa tttcatctgc atgaccaata atatctacag ttgcagttgg
    13861 gttatttctt aaataagtta agataaaatc aattccttca gtagaaacat tagttggttt
    13921 cgttttgttg aaatcaaaat aagtagctac atagccttcg ttgattaatt ttttaacggt
    13981 ttctttttca gagttaacta acatttgatt ggtagtgtaa gtactgttta agtatctttc
    14041 catttcgtcc ggaacaccgt ttttgtttaa gtcaaccatt ctacctttgg tatctacagc
    14101 aataccggca attgaatttg gttcaacatc taaataatca ggcacaccat cttgatctgt
    14161 atcacccata ttattttcaa tttcaccaac acgtttttca agagcttcaa tttcttcttg
    14221 ttgtttatct cttaagacag cccaatctcc gtgaattttt tcagcaccaa aagagaagga
    14281 agctcctaac gatatagtat ataaacttcc tgctaaatta ttgtttggat cagaatatgc
    14341 tccatcccaa ttgaaatgtt gtcttacata agaattcatc gtaaaatctg cattcattgc
    14401 taaatgttta gtgattctaa attcaggagt aaatccaaac ataataccac cgttccattc
    14461 atctctatct ttattaattc ccatttgagg agtcatttgt gccacttgaa tacctccatg
    14521 gaataagaaa ctaaatcttc ctaaaggttc ttgaatatca aataatcgag ccgcattaac
    14581 aacaccttga acaccaaaac ggatgtgttg catttcaaaa tctaaacttt catcgcttaa
    14641 attttctaaa cgatcaaaag caacatctgc ttttaaacca aatttaggac taaacatata
    14701 acgaacacca ccactgtagt gatttaaagt tacagaacca aaagatttag ttggatcact
    14761 tccaaaatag ccaatagagt aaggtttaat acctttactt tgacctaggt taaattcaaa
    14821 agaccatttg ttgtattcat cattaagatc agttttaagg gtatctttgt cttgtgcgaa
    14881 agattgtgtg agggtcaaaa ctgaaaatga aattaatagt ataagtttct tcattttaaa
    14941 ttgtatcaag ttagatttgt taattgctgc aaatatagag caaatgttga aataatcaag
    15001 aataattgtt aaaataataa aattaaacag ttaattaaaa acaagttaga atgttaaaat
    15061 tgaattaata tgaatataaa ccatgaataa ctaaatattt atttgtaatt gaattgatac
    15121 aatgctgttt taataaaaat tgcgaaatcc ttttcggtta atgatttcat aaaattttat
    15181 ttttagagta aagtaaaatt taaatttacc taaaaaaaac atgagatttc acacaagaaa
    15241 atgggttaaa cccgaagatt tgaatccaaa tgggacttta tttggaggaa ggttattggc
    15301 atggattgat gaagaagtgg ctttgtattc cattatacaa ttagaaaacc cgagagtggt
    15361 aaccaaacac atgtctgaaa taaactttaa gagttcagct aaaacgggtg atattataga
    15421 gattggtatc gatgttgtaa aatttggaaa aacctcaatt actttatctt gtcaagtgcg
    15481 taatatcatg acgagagaaa taataatttc cgtcgatact attacaatgg taaatttgga
    15541 tcaagaaggt aaaccattag tacatggaaa aacaaaaata gaatttatca ccgaacgatt
    15601 agataaataa tcaatcttta ttaatatatt tactttcaat ataaaatgtt ccaaatggaa
    15661 taatggacgc taaacaaatt ataccaaact ttttataatt ccaaggactg gaaaacttta
    15721 aggttaatgc taataatata tataaaataa ataataaacc atgagccatt ccgatggttt
    15781 ttacaaaaat atcctgaccc gccaaatact tcattggcat ggcaaaaaat aagagtagaa
    15841 tgagtgataa tccttcgaac caagctgttt ttctgaatat ttctagcatg ttataacatt
    15901 tgataaatgg caaaaccgta aattacaaat cgcatcaaac gcaataaggc aaataaaaga
    15961 aaatttttga aattgaattt tatcatccct gcagccattg aagccatagc aaaaggcaat
    16021 ggtaataaag ccccgacagc aatcaataaa ccgccccatt ttcgcatatt gataatatgt
    16081 tttgtcattt tttcagacat gaatttatta accgaaggaa tataaagaat tgcttttccg
    16141 gttatataag atactactcc tcctaaatat gaaagaattg ctaataacga aagataaata
    16201 agaggaaagt ctgtgttttt tgtccaagct ataaaaatat caggcggcaa aagtccaaga
    16261 atggattcgg aaataaaaaa tacggagaat atagcaaaat cactatatcg ttctgttaac
    16321 attattaacg cctcatttat atttattaca taataatgaa cataaaataa agcaccaaaa
    16381 atgagtaggg taggtaaaaa agcacttttt attccttcaa aaatgaattt gtaaaatccg
    16441 gtgtaagtgt aataaaggtg aagttgtttc cacttatttt ttttaattcg ttctctttgc
    16501 ataattaatg aatatcactt tctttaatga taatagttgc tttaggttta ataaaatata
    16561 aatacaagaa tccaatagta cctccggtta aagaggcaat aaggattcca atttttgctt
    16621 gttgaattaa ttcattattg tcaaaggcta aatttgctac aaataaggac atagtaaatc
    16681 cgatagaagc taataatcca acaccaatta aatgtttata attcattcct tccggtaatg
    16741 atgccagttt taatttgaga aacaaacttg ttaccccaac aattcctatg aatttaccga
    16801 ttaatagccc gaaaatcact ccaattgcta cataacttga agttgtagta gctgtttcag
    16861 atgataatgt aactccggca ttggccaaag caaaaatggg cataattata aaagtaacta
    16921 ttggatgaag gccatgctct aatcgttgaa gtggggtcat tgcttgttta gacaatgtac
    16981 gcatgtcttc taaaatatga agctgttctg gagtaacagt tggtactcca ttagggtcgg
    17041 cttttttaaa tttttctaaa tacattttca ttttttccga aaaaccaatc tcatcaattt
    17101 tgacatttgc cgggattgta aaagcagcta aaacgcctgc aatagtggca tgaacaccgg
    17161 agagtaaaaa agccatccac aatccaccaa taccgagtat accataataa attgtatttc
    17221 ggacaccaac cagatttgaa ataattaata atagaagaaa ggcagtacca ataattaaac
    17281 tggtaaatga aatatctgat gtatagaaaa aggctattac caatacagct ccaatatcat
    17341 cagctatcgc caaaaccgtt aaaaacagtt tcaacgaagt aggaacttta tcacccaata
    17401 aaaataaaac acccaaggca aaagcaatat cggttgccat tggtattccc caaccgcttt
    17461 gagcttcccc ggtggaatta aatagtaagt aaattagagc tggaaaaatc attccaccaa
    17521 atcctgccgc tacaggtaga aaagcttttt ttggatttga aagttcacca ccaattattt
    17581 cacgttttaa ttcaagccca accacaaaga aaaaaacggc cattaaacca tcatttatcc
    17641 aatgatgtaa atcttggtct aaaaggtatt tattatcaaa gcctaaactg aatttgtgtt
    17701 cccaaaaatg atgataagct tctttccaag gtgaatttgc taaaattaat gcaacaactg
    17761 cagctaaaaa aagtacaatt cctcctgttg ttgatttacc tataaaccga ttgaccgggt
    17821 taattaccca tttatcaatt ggtgttattt ttaatcggta tttcattaat aagccttgtt
    17881 tttttaaggg ttgtaaaaat aggaaagttc attgattatt cagaatttta aaagtcattt
    17941 tctctcaatt tatatttaat ttaacctttg tcactttttt ttttaattta ataactttaa
    18001 aattcgtttt caggctttta tctgcttatt tttgcaaaat tcaatttttc aattttaaaa
    18061 tgtcaaaaaa agatatattg cttgaatatg aagagaagaa gaaactctac agttatcaat
    18121 taggtgatat agaccttatt tttgataaaa ttgaaacggc accacataat tatcatttac
    18181 tgtatcaatt accaactggc ggagggaaaa ctgtcatttt ttcagaaata actcggcgat
    18241 atttagaaaa atatgataaa aaagtggttg ttttaacgca tcgaattgaa ctttgcaagc
    18301 aaacttcaaa aatgctgaag ggatttgatg taaaaaataa aattattaat agtgttgtta
    18361 aagatttgcc cgatcaaaat gattattctt gttttgtggc catggttgaa actttgaaaa
    18421 ataggttaaa tgatgaaaaa ttgcatattg ataatgttgg tttgttaatt attgatgaag
    18481 ctcactttaa ctcatttcga aaattatttc atgcttttaa aaatacattt ttcttaggtg
    18541 ttactgctac tccgctcagt tcaaatatga aattacctat gggtgaaaat tatcatgaat
    18601 tggttgttgg ccacagtatt gcgtccttga ttgataaagg ttatttggct aaagcaacta
    18661 cgtatagtta tgatgtagga ttaacttctt tgaaaattgg tattaatggc gattatactg
    18721 taaaatcatc ggatgaattg tatactaatt tggccatgca agagaaattg atacatgcct
    18781 acagtgctca ttcgatggga aagaagacgt tgatttttaa taatggaatt attacttcac
    18841 tgagcgttct tgacactttc agggaagcgg gatttaaaaa tatcaagcat ctagacaata
    18901 caaactctaa cgaggaacga aaggatattt taaaatggtt taaagatact ccggatgcca
    18961 tattaacctc ggttggaatt ttaaccacag gttttgatga gccaactgta gaatcgatta
    19021 ttttaaatcg agcaaccaag tcactgactc tttattttca aatgatagga aggggttctc
    19081 gaaaactacc taataaagat gctttttcta taatagattt aggaaataat gcagccagat
    19141 ttggtttgtg gacagataaa ttggactggc agcatatttt taagtatcct gaacaatata
    19201 ttcataatct caagggagat gtggaaattg aaatgaattt ttattataca atgccggagg
    19261 agttaaaagc taaattcaga aatactcccg atataacttt tgacgtagaa actgaatatc
    19321 gaaaagcatt atctaaaaat ttaagaccta aagttgtaat cgaaaaatca attgaacatc
    19381 atgcttccat gtgtgtagat aattcagaaa cattggttga agcaaagcaa ttagcaaaag
    19441 aactagcaga agatatcgaa agcagagttc gacattttac gaattgttta agcagttcta
    19501 ccaagaacta cagagaatgg ctgattgaag attacaaaac aagacttact gtaacaattg
    19561 gtcgaaaatt tagagaaaaa atcttttcga tggactaaat cggtccattt ttaatgttaa
    19621 aatttgattc ttcatcactt tttatttcaa ttcgattgcc gataaacgat agaataagtt
    19681 actcgtctag agcactatgc atttcgtcga tgaaatgagt gttt
//
LOCUS       DAOTCW010000034        19543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1514_length_19543_cov_37.267293, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000034 DAOTCW010000000
VERSION     DAOTCW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 19543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19543
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1514_length_19543_cov_37.267293"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(638..1768)
                     /gene="ribB"
                     /locus_tag="P5335_04440"
     CDS             complement(638..1768)
                     /gene="ribB"
                     /locus_tag="P5335_04440"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRZ74161.1"
                     /translation="MSYKTQLNTIEEAIEDIRQGKIIIVVDDEDRENEGDFLAAAEKV
                     TPEMINFMATHGRGLICAPLTERRCSELELRSMVVNNTDHMETAFTVSVDLKGNGVTT
                     GISAADRAKTILALVNEETKPFELARPGHIFPLIAKQGGVLRRTGHTEAAIDFARLAG
                     FSPAGVICEIMNEDGSMSRLPQLVDVAKKFDLKLVSIEDLVAYRMQHDSLIQKKEDFE
                     INTRFGTFRLRAYLQTTNKQIHLALTKGSWNNGEAVLTRINSKQVNNDILETLTNNTD
                     KRLEDMFNRINKEGRGAIIFINQEIPSIELLNRICELKILQEQGEMKAPPIKMDSKDF
                     GIGAQILHDIDISKIRLLSNSEQTKRVGMIGYGLEITEYVKY"
     assembly_gap    1901..2000
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2210..3616)
                     /locus_tag="P5335_04445"
                     /pseudo
     CDS             complement(2210..3616)
                     /locus_tag="P5335_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
     gene            complement(3624..4265)
                     /locus_tag="P5335_04450"
     CDS             complement(3624..4265)
                     /locus_tag="P5335_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRZ74162.1"
                     /translation="MKNLIWISIFFISTITSFSQEKKAKDLLDEVTAKVKSYENIAID
                     FKYSLQNTKENINQDSKGNVVLKGNLYYLNFMGVTKIFDGKKTYTIVPEDEEITISKV
                     DDKDENAITPSKMLTFFNNGYKYYWDILQDVKGRKIQYIKLVPISSKDQRKEILLGID
                     VQTKHIYNLIEMGKNGTKTTLTVNSFKTNQPVSKNQFTFDETKYKGYYINRID"
     gene            complement(4267..6708)
                     /locus_tag="P5335_04455"
     CDS             complement(4267..6708)
                     /locus_tag="P5335_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRZ74163.1"
                     /translation="MAKSSTTSSAKSETDKETKKSWKFTKQHKFLFGCFLVLFSVALF
                     LSMISFFIYWKEDQSAITSLTNRSVEVHNWLGKIGAYIADFFLYKGFGIASFLLVRLF
                     FLSGIYMILSLPLKKLKNVWFWDLFAMVNFSLLFGFFSNYIPELGGVIGFEMNSLIQD
                     FMGKPGTLLVLVFSFLIYLIFKVNVSPETFQKLFERKKKEIKEDLADAKSAFDQPLPT
                     NTKEEEEVEEGVISMKPTSVFEINKEALKPTIEHASEINLEPKVKTLDPLPHSVPREP
                     EIIHTDDEQFVIEKAPEEEQLEENLAARLVADFGEFDPTLELSKYKFPTLDLLKDYGS
                     AGITINQEELEENKNRIVETLRNYKIDIAQIKATVGPSVTLYEIVPEAGVRISKIKSL
                     EDDIALSLSALGIRIIAPIPGKGTIGIEVPNKNPTMVPMKSVLSSQRFQEAEMELPIA
                     LGKTISNETFVVDLAKMPHLLMAGATGQGKSVGLNAVLTSLLYKKHPAEVKFVLVDPK
                     KVELTLFNKIERHYLAKLPDTSDAIITDNTKVINTLNSLCIEMDNRYTLLKDAMVRNI
                     KEYNEKFKNRKLNPNEGHRFLPYIVLVVDEFADLIMTAGKEVETPIARLAQLARAIGI
                     HLIIATQRPSVNVITGLIKANFPARIAFRVTSKIDSRTILDTQGADQLIGRGDMLYSN
                     GNDLVRVQCAFVDTPEVERITEYIGSQKAYPNAYLLPEYVGEENGTMLDIDISERDSM
                     FREAAEVIVIAQQGSASLLQRKLKLGYNRAGRLIDQLEAAGIVGQFEGSKARAVNIPD
                     LNSLNEFFNNEQNNA"
     gene            complement(6754..7137)
                     /locus_tag="P5335_04460"
     CDS             complement(6754..7137)
                     /locus_tag="P5335_04460"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRZ74164.1"
                     /translation="MEFQKDTTLFTGRLKSMVFALKGAYKLVTTEHSVMVQFSIGIII
                     TIAGFYFEITKTEWLLQTLAIGLVLSIEGLNTAVEKIADFIHPEFHERIGFIKDIAAG
                     AVFFAAMTAIVIGAIIYFPYVTTLV"
     gene            complement(7145..7642)
                     /gene="tpx"
                     /locus_tag="P5335_04465"
     CDS             complement(7145..7642)
                     /gene="tpx"
                     /locus_tag="P5335_04465"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRZ74165.1"
                     /translation="MASITLGGNPIHTQGELPKAGSKAPDFNLIKTDLSSTSLSDYAG
                     SKLVLNIFPSIDTGVCATSVRTFNQKASSLENTKVLCISRDLPFAQKRFCGAEGLENV
                     ENLSDFKTGSFGDAYGLTIVDGPLAGLHSRVVIVLDENGMVTYAEQVPEIAQEPNYEL
                     ALAAL"
     gene            complement(7760..8017)
                     /locus_tag="P5335_04470"
     CDS             complement(7760..8017)
                     /locus_tag="P5335_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74166.1"
                     /translation="MKLPVIKHLTQFIEDNDQDYLVETIEVLEALTEIPSLKEEELDV
                     IGELISNMYGALEVQKSIQNGMDKKEALNGFMKRVLGSIDK"
     gene            complement(8017..8331)
                     /locus_tag="P5335_04475"
     CDS             complement(8017..8331)
                     /locus_tag="P5335_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRZ74167.1"
                     /translation="MLIDLNEDTLQDLVTTNNKVVVQYSASWCGNCRIMKPKFKKLAS
                     ENDAITFVLVDAENSPESRKLANVSNLPTFATFVNGELVNQTQTNKAEVLAELVSEIS
                     AN"
     gene            complement(8401..9039)
                     /locus_tag="P5335_04480"
     CDS             complement(8401..9039)
                     /locus_tag="P5335_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRZ74168.1"
                     /translation="MSLVGKKFPNITVDAISEMGDNLRINVLDEAVKNNKKVILFWYP
                     KDFTFVCPTELHAFQTALPEFEKRNTVVIGASCDTNEVHFAWLNTAKNNGGIEGVTYP
                     ILADTTRNLSSALGILDIEVGEYNEESDSYLIDGSNVTYRATYLIDETGKIFHESVND
                     MPLGRNVNEYLRLIDAYTHVQTKGEVCPANWEEGKDAMVADRLSTAAYLSQN"
     gene            complement(9322..10803)
                     /gene="nhaC"
                     /locus_tag="P5335_04485"
     CDS             complement(9322..10803)
                     /gene="nhaC"
                     /locus_tag="P5335_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HRZ74169.1"
                     /translation="MESQDIKSQDPKDEQLIENKELSIFEALIPILLLIAILAYNVYA
                     YGDSATAGPNQFALIIGGAIAAIVGFLNKVSYQAMMEEIAKNVKSTAGAILILLMVGA
                     LSGTWLISGVIPTMIYYGLQILHPAIFLPACLIICSVISIATGSSWTTSATVGIALIG
                     IGSTIGMPLGMVAGAVVSGAYFGDKLSPLSDTTNLAPAMAGTDLFTHIRYMTITTVPT
                     MIITLLLFIFLGLAQTTNGSGDNSATLTSIKDAFTITPWLFLVPGIVIILIIKKTEPL
                     IALLIGTLLGGVFALIFQPELVAKIGGSNHLSFVSGYKGVMNAITVETSVETTNQMLS
                     DLFSAKGMKGMMSTIWLILCAMVFGGVMEAIGALTRITDSLLKLFHTTFGLFASTVAS
                     CLALNLTASDQYLAIVVPGKMFAKAYQDKGLAPENLSRTLEDSGTVTSALIPWNTCGA
                     YQSGVLGVSTISYLPYAFFNIISPFMTLLFAAFNIKIKQLTTK"
     gene            complement(10909..12123)
                     /locus_tag="P5335_04490"
     CDS             complement(10909..12123)
                     /locus_tag="P5335_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRZ74170.1"
                     /translation="MKPTDFNAANQIQDLQYFGEFGGVNPSISDSSTYTFLSAKTMFD
                     TFEGNAEGCYLYSRHSSPMNLYLGKALAAMEGTESANVAASGMGAITPVLMQLCNAED
                     EIVSSRTIYGGTYAFMKNMMPRWNIKTHFVDITKLDIVEAAINQNTKVIYCETVSNPL
                     LEVADIEGLAKIAKKHNLKLVVDNTFSPLSVAPAQLGADIVIHSLTKFINGSSDTVGG
                     VVCASQEFINALKNVNSGASMLFGPTMDSLRSASILKNLRTLHIRMKQHSYNTHYLAE
                     KFEKDGLKVVYPGLKSHPSYELYASMINKEYGFGGMMTLDVGSLDKANELMELMQNRN
                     LGYLAVSLGFYKTLFSAPGTSTSSEIPIEEQKEMGLSDGLIRFSIGLDNDIERTYQKM
                     KACMIELGVLNK"
     gene            12296..12757
                     /locus_tag="P5335_04495"
     CDS             12296..12757
                     /locus_tag="P5335_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRZ74171.1"
                     /translation="MELDKIDKKLLSLLQTDTKQTTKELSLLLNLSVTAVYERIKKME
                     RLGIIQQYVALLNRSKINKGFVVFCHVKLVQHTREYISDFESEVVKLHEVLECFHVSG
                     DYDYILKICVGNMEEYREFMITKLTTLNHIGSTHSSFMIGEVKNTTVFELV"
     gene            12839..13987
                     /gene="bshA"
                     /locus_tag="P5335_04500"
     CDS             12839..13987
                     /gene="bshA"
                     /locus_tag="P5335_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HRZ74172.1"
                     /translation="MKIAIVCYPTFGGSGVVATELGLKLAHRGHEIHFITYRQPVRLA
                     LLNPNVHYHEVNVPEYPLFHYQPYELALSSKLVDMVKLHKIELMHVHYAIPHAYAGYM
                     AKQMLKDEGIYIPMVTTLHGTDITLVGNHPFYKPAVSFSINKSDVVTSVSQSLKDETY
                     KLFNITKEIEVIPNFIELEKTKLEEGAPCYRSLMATDSEKIITHISNFRKVKRIPDII
                     KIFHKIQQQIPSKLMMVGDGPEKEKAELLCEELGIANKVIFFGNSNEIDKILCFSDLF
                     LLPSETESFGLAALEAMACSVPVISSNTGGLPEVNVDGFSGYLSNVGDIDSMAENAVR
                     ILENETNLASFKKNAYTVAERFDIAKILPLYEAIYEKAIQEQFKTESN"
     gene            13989..14411
                     /locus_tag="P5335_04505"
     CDS             13989..14411
                     /locus_tag="P5335_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HRZ74173.1"
                     /translation="MKKIIAFLIISIVLFSCASKTNNADKLPLYEILKQEDQGGANFK
                     FFEIISEPKEFKMLLNDADLKKKIKAADIYTCNFLLLNMGTKSSGGYYFTIENIEEME
                     NDIVITIKENEPKGMATSVMTNPLAIVKINSKKNIIIK"
     gene            complement(14482..14970)
                     /locus_tag="P5335_04510"
     CDS             complement(14482..14970)
                     /locus_tag="P5335_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74174.1"
                     /translation="MKKLFLSALILVGFTFSADAQEISKNALGLRLGSNDGFGAEISY
                     QRKLSNSTRLEFDLGLRNSDNDDAFKIVGLHQWVWTIEKRFQWYAGLGAGLGTWTNND
                     KNHEYEDGAFLLMAGDIGIEYNFDIPLLLSLDFRPELYFGNNYRNDNYGSDIALSIRY
                     QF"
     gene            complement(15046..>15750)
                     /locus_tag="P5335_04515"
     CDS             complement(15046..>15750)
                     /locus_tag="P5335_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74175.1"
                     /translation="NYFPLTSDNSWTYEVEGQTTDFDNLYVGDEIILNNKSYQQMKTT
                     DVPFGFYSSLLRDNGLRNEGQKLLLTGTLALDLGVDIPLDFALEDFILINNDATSNEV
                     MSTKSGTISQDLNGTPITIDYTIKSTALETLPSFTTSKNVTYTDVKKVNFVINVEVTT
                     IQTIPGTNITIPIPILNSQDVLTATHYYAQDIGMVYSNNQISYSLAINPADFGLDIPQ
                     TSSQSQNEFLDSYTVN"
     assembly_gap    15751..15850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16033..16863)
                     /locus_tag="P5335_04520"
     CDS             complement(16033..16863)
                     /locus_tag="P5335_04520"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRZ74176.1"
                     /translation="MLKKRKVEVVVISDVHLGTFGCHAKQLVHYLSTIKPKILVLNGD
                     IIDIWQFRKSYFPKAHLKVIKKIIDMASKGTEVYYITGNHDEMLRKFSDTKIGNFSIL
                     DKLVLELDDKKAWIFHGDVFDASVQHSKWIAKLGGWGYDLLILVNRFVNRILEKMGKE
                     PYSFSKKIKANVKKAVKHISDFELTASELAIKNNYDYVICGHIHEPKMIRNVTKKGTT
                     FYLNSGDWVENLTALEYNKKRWKLYRYDKDTNMIEEDFFEMESKLSEQLIASLIFSQK
                     "
     gene            17024..>18062
                     /gene="aroC"
                     /locus_tag="P5335_04525"
     CDS             17024..>18062
                     /gene="aroC"
                     /locus_tag="P5335_04525"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRZ74177.1"
                     /translation="MAGNSYGTLFKLSTFGESHGDALGGIIDGCPPGIHLDFEAIQFE
                     MSRRKPGQSNIVTQRKEADEVQFLSGIFEGVTTGTPIGFIIPNSNQKPTDYAHLKEAF
                     RPSHADFTYEKKYGIRDYRGGGRSSARETTCRVVAGAIAKQVISSIKINAFVSSVGSL
                     FIDKPYQELDFSKIENNPVRCPDEKTAALMEDYIKEIRKQGDTVGGTITCVIQNAPIG
                     LGEPVFDKLHAELGKAMLSINAVKGFEFGSGFCGAQMKGSEHNDLFNADGTTKSNLSG
                     GIQGGISNGMDIYFRVAFKPVATIMQKQETIDNKGKIVELEGKGRHDPCVVPRAVPIV
                     EAMAALVLADFF"
     assembly_gap    18063..18162
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18247..19524
                     /locus_tag="P5335_04530"
     CDS             18247..19524
                     /locus_tag="P5335_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRZ74178.1"
                     /translation="MKLALHWKILIGMVFGLAFGFVMKNLEMKGIVVDWIKPFGTIFI
                     NLLKMIAVPLIIVSLIVGLSDLKDISKLSKLGGRTVVLYLVTTVTAVSIGLVLANLIK
                     PGEYINDESRKSLLENFAGDAAKKIELAASAKNQGPLQPLVDVVPENFFLAMTDNASM
                     LKVILFVILIGIGLILIDENKAKPVVDFFKGLNEVILKIIDIIMLFSPIGVFALMAAL
                     MVEIPDFSTLNALGIYALTVVLGLIIMTFVIYPTMLIVLAKYSPVKFFRAIAPAQLLA
                     FSTSSSAATLPVTMERVIENVGVDEEVASFVLPLGATVNMDGTSLYQAVAAVFIAQAM
                     GIPLDLQTQLMIIVTATLASIGSAAVPSAGMVMLVIVLGQAGIPEAGLALIFAIDRPL
                     DMCRTVVNVTSDAVVSTVVAKSVGKLNPINTEV"
BASE COUNT         6309 a         3204 c         3199 g         6531 t
ORIGIN      
        1 taacagacta tttgacattc aatggtataa atgacataat taaacacaaa tgacatctta
       61 ttttgaataa atattaaagt aaattgcacc attaaattga aaaataaatt tcattaactt
      121 taaattctga aaagaatata taattaaacc taaagattac gatacctcta atcaataccc
      181 tagataatgt atgagaacag aaccgaaacg catttgcatc tacccgaaag acatacaacg
      241 tataacaggc aagtcctacc ggcaaagcat aagaatgctt caaaaaatcc gtaaagacct
      301 tagcaaactt gataacgagc tagtgagcat agaagagttt tgtcaataca ctagcttaaa
      361 atatgaacaa gtagaaccat taattattgg ttaaactacc ttaaacttgt atagatattg
      421 aactactatt taacaattat ttcctatatt tgtgtaataa gatttgtcaa aaaagattgt
      481 tttttttgag gttcaaaatc gagaccctag aatttttatt cagcaataaa gcttataaat
      541 aaaggaaagt ctgaggaaat acaaatccct ctttctccgc aaaagaaaaa ccgattagga
      601 aactaatcgg tttttttatt ttataaggat aatatttcta atacttcaca tattctgtaa
      661 tctccaagcc ataaccaatc attccaactc gtttggtttg ttcagagttg gatagcagtc
      721 tgatttttga aatatcgata tcatgtaaaa tttgagctcc aataccaaaa tcttttgaat
      781 ccattttgat tggtggtgct ttcatttctc cttgttcttg aagaattttt aactcacaaa
      841 tacggtttaa taactcaatg gaaggaattt cttgattaat aaaaataatt gctccacgac
      901 cttctttgtt aatacgatta aacatatctt ccaatcgttt gtctgtattg tttgttaagg
      961 tttctaggat gtcattatta acttgcttgg aatttattct ggtaagcaca gcttccccat
     1021 tattccaact tcctttggtc aatgctaaat gaatttgttt gtttgtagtt tggagataag
     1081 ctcgtaaacg aaacgttcca aatcgggtat tgatttcaaa atcttctttc ttttgaatta
     1141 aactatcgtg ttgcatacga taagccacta aatcttcaat tgaaactaat tttaaatcaa
     1201 actttttagc aacatctact aattgtggta ggcgagacat cgaaccgtct tcattcatga
     1261 tttcgcaaat tactccggca ggggagaacc cggctaagcg tgcaaaatca atagccgctt
     1321 ccgtatgccc tgttcttctt aaaacgccac cttgttttgc aatcaatgga aaaatgtgtc
     1381 ctggacgagc tagttcaaac ggcttagttt cttcatttac caatgctaaa attgttttag
     1441 ctctatctgc tgcagagatt ccggttgtaa cgccattccc ttttagatca acagaaacag
     1501 taaatgcagt ttccatatgg tcggtattat tgactaccat ggaacgaagt tctaattctg
     1561 aacaacgacg ttctgttaag ggagcacaaa tgagtccgcg gccatgtgta gccatgaaat
     1621 taatcatttc cggagtaacc ttttcagccg cagccaaaaa atcaccttca ttttcgcgat
     1681 cttcatcgtc aactactatg atgatttttc cttgacggat atcttcaata gcttcttcta
     1741 tggtatttag ttgtgtttta taagacatga ttaattttta tgctttcaaa agttattatc
     1801 gatattgagt catcgcttct ttaatgactg ttttaatata aaaatgtaat aaaataaaga
     1861 agggaaatcc aactaaaaaa gttatgacta ggcctacatt nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn gtaatgccac gattagataa tatttttaag gctttatttc
     2041 taaaatcgct tgaataatta aactgtttag aattttttac aatattgatg agtgaatcat
     2101 ccgtgtagtg atttaatttt tcaaattctt catcttcatc tatttttagg gcatctaaat
     2161 ttacaattgt ttctgatagt atgccttctt cattatcttt tggagcaaat aatttttgaa
     2221 atggtattgt aatccaatca aaattaagca ttaatccaat atcatttgta gctctgtagg
     2281 ttaataaaac agcgaaagga gtgaggatga tggaagacat ccatgaacct aaaaagggag
     2341 gaattccatt ttcttgagcc acttttttac caaatacatt gataaaatgg taaataatga
     2401 atactaccat ggcaaaaact attgggagtc ctaatcctcc ttttctgata atagctccta
     2461 taggagcccc aataaaaaac attaataaac atgcataagc aataacaaac ttgtcaaata
     2521 aagctaacca atgattatta atatttttta tttttccggt aaattcatat tgtgaaccat
     2581 caattgaata ttttgtgttg gtaacacttc cttttgcaaa ttcaagaatt tgaacaactt
     2641 gataaggttt taagatggaa gttaagtctg caatgatggt atctttaatt tttggtttgt
     2701 caacaagctt ggaatttact aatccaattc gttggtgaat attttctgta taagatacaa
     2761 tatctttttc tttttttgtt tctaatgaat cgattgtata aaccaattca ttgacattta
     2821 gcattgtatt ggtattggtg atttcatccg tattcatatc cgttttttgc aacggagcta
     2881 aatcaaaatt aataatgtat tttttgaagg aactttttgc aaaaggtaat ttctttttgt
     2941 cttcaaaatt tttaggttga atatcttcgt aataattacc gttgtagaga atcaattgaa
     3001 ggaaatttgt tttttcatcc ccaataagtt cgccattttt agctttgata attacatttg
     3061 ctcctacacc aaaatttgaa agacggtgaa tggtaatgtc atctaaaaga ttgttatttt
     3121 ctccgtgttt cttgcttact ttaatgttgt aactaccaat tttgctgaat tgaccttccg
     3181 ctatggccat cgatggcttt tgctgaacaa ttgtttttcg taggtttaca aattcatatt
     3241 ctgcttgtgg aatgatatta ttggcaaaca aaaaagcaac aaaacttagt ccggatataa
     3301 aaattgctaa ggaatacatt gctcttttta aagaaattcc ggaagctttc atggcagcaa
     3361 actcatagtt ttcggcaaaa tttccgaagg tcatgatgga ggacaataaa acagaaagtg
     3421 gtaaaactaa gggtacaacc ttaggcgaat aaaataataa gaactttata attaaataag
     3481 tatctaaatc tttcccggct aaatcagcaa tgaagagcca aattccctgt aaaataaaga
     3541 taaaaaaaag gattacaaat accgtagcaa atgtactcag aaacgaagtc agtatatagc
     3601 gatccagtat tttcacctca gaattagtct attctattga tgtagtaacc tttgtattta
     3661 gtctcgtcaa aggtaaactg attttttgat accggttgat tggttttgaa agaattaaca
     3721 gtgagggtag ttttggtgcc gtttttaccc atttcaatta agttatagat atgttttgtt
     3781 tgcacatcga tccctaaaag tatttctttt cgttggtctt tcgaactgat tggcaccagt
     3841 ttgatgtatt gaattttacg tccttttaca tcttgtaaaa tatcccaata atacttataa
     3901 ccattattaa aaaaagttaa cattttagag ggagttatcg cgttttcatc tttgtcatcc
     3961 actttagaaa tggtgatttc ttcatcttca ggaacaatag tataggtctt tttgccatca
     4021 aaaattttgg taacacccat aaaatttaaa taatataaat ttcctttgag cactacattt
     4081 cctttgctgt cttggttgat attttctttg gtgttttgaa gagaatattt aaaatcaatg
     4141 gctatatttt cgtagctttt aactttggcg gttacttcgt caagtaaatc tttggctttt
     4201 ttttcttgac taaaagaggt aattgtagag ataaaaaaaa tagaaatcca aataagattt
     4261 ttcattttaa gcattatttt gttcattatt aaaaaattca tttaacgaat tcaaatcagg
     4321 aatattgacg gcacgtgctt tacttccttc aaactgacca actattccag cggcttctaa
     4381 ttgatctatt aagcgaccgg ctcgattgta acctaatttt aattttcgtt gtaataaaga
     4441 agcagagcct tgttgagcaa ttacgatcac ttctgcagct tctctaaaca ttgaatcacg
     4501 ttcagatata tctatatcaa gcattgtgcc attctcttct cccacatact ccggaagaag
     4561 atatgcattt ggataagctt tttgtgaacc gatatattct gtgatacgtt ctacttccgg
     4621 tgtgtctaca aaagcacatt gcacccgaac taaatcattt ccgttggaat agagcatatc
     4681 gccacgacca atcaattgat cagctccttg cgtgtctaaa atagttcttg aatcaatttt
     4741 tgaggtaact ctaaaagcaa ttctcgcagg gaaattagct ttgattaaac cggtaatcac
     4801 attcacagac ggtctttgag tagcgatgat taagtgaata ccaattgctc gtgctaattg
     4861 tgccaaacgg gcaataggtg tttccacttc ttttccggca gtcataatta aatcggcaaa
     4921 ttcatccact actaaaacaa tatagggtag aaaacgatgt ccttcgtttg gatttaattt
     4981 tctgttttta aatttttcgt tgtattcttt gatatttctc accattgcat ccttcagcaa
     5041 agtatagcgg ttatccattt caatacaaag ggaatttaag gtattgataa ctttcgtgtt
     5101 atcggtaata atagcatcgg aagtatccgg taatttagct aaataatggc gttcgatttt
     5161 gttaaacaaa gtcaactcta cttttttagg gtcaaccaaa acaaatttta cttccgccgg
     5221 atgtttttta tacaataagg aggtaagtac agcattcaaa cctacagatt ttccttgtcc
     5281 ggtggctcca gccatcaaaa ggtgaggcat cttagctaaa tcgaccacaa aagtttcatt
     5341 agaaattgtt tttcctaaag caatcggtaa ttccatttcg gcttcctgaa aacgttgaga
     5401 agacaaaacc gatttcatcg gaaccatagt aggattttta tttggcactt caataccaat
     5461 ggttcctttg cctggaattg gagcaataat acgaattcct aaggcagaga gtgataaagc
     5521 aatatcatct tctaaacttt taattttaga aatacgaaca ccagcttcag gaacaatttc
     5581 atataaagtt actgatggtc caacggttgc tttgatttga gcgatatcaa ttttataatt
     5641 tcgaagtgtt tcaacaattc gatttttatt ttcttctaac tcttcctggt taatggtgat
     5701 tccggctgaa ccgtaatctt ttaataaatc tagggttgga aatttatatt tagaaagttc
     5761 taaagttgga tcaaactcac caaaatcggc tactaaacga gcggcaaggt tttcttctaa
     5821 ctgttcttcc tcaggtgctt tttcaatcac aaattgttca tcatcggtat ggatgatttc
     5881 aggctctctt ggaactgaat ggggcaatgg atctaatgtt tttacttttg gctctaaatt
     5941 gatttctgaa gcatgttcaa tggttggttt taaagcttct ttattgattt caaagacaga
     6001 agttggtttc atagaaatta caccttcttc aacttcttct tcttctttag tattggtggg
     6061 taaaggttga tcaaaagcac tttttgcatc ggctaaatct tccttaattt cttttttctt
     6121 ccgttcgaac aatttttgaa aagtttcagg ggaaacattg actttaaaaa tcaaataaat
     6181 taaaaaactg aaaactaaaa ctaacaaagt tcccggtttt cccataaaat cctgaatcaa
     6241 ggaattcatt tcaaaaccaa taacaccgcc tagttcagga atgtaatttg aaaagaagcc
     6301 aaaaagtaat gaaaaattaa ccatggcaaa taaatcccaa aaccaaacat ttttgagctt
     6361 tttgagtggt aaactcaaaa tcatataaat tccggataaa aagaaaagtc ggactaataa
     6421 aaaggaagca ataccaaatc ctttataaag aaagaagtcg gcaatataag caccaatttt
     6481 tcctaaccaa ttatgaactt caactgaacg gttagttaaa ctggttatgg cactttggtc
     6541 ttctttccaa taaataaaga aagaaatcat cgaaagaaaa agagcaacag aaaataaaac
     6601 caaaaaacaa ccaaacaaaa atttgtgttg tttggtaaac ttccaggatt tttttgtttc
     6661 cttatctgtt tctgattttg cagaacttgt cgttgatgat ttagccattt aaaaactgtc
     6721 tgatttgctg taaaagtaat tatttaaatt tagttatact aaagttgtaa catacggaaa
     6781 atatatgata gcaccaatta caatagcagt catagctgca aaaaaaacgg cacctgctgc
     6841 aatatcttta atgaaaccta ttcgttcatg gaattccgga tgaataaaat cagctatttt
     6901 ttcaacggct gtatttaatc cttcaatgga caaaactaat ccgattgcta acgtttgcaa
     6961 aagccattct gttttggtaa tttcaaaata aaaaccggca atagtaataa taataccgat
     7021 tgaaaactga accatcacgc tgtgctctgt tgtaaccaat ttgtaagctc cttttagagc
     7081 aaaaaccata ctttttaggc gtccggtgaa aagggtagtg tctttttgaa attccatatt
     7141 cgaattataa tgcagctaaa gccaactcat aatttggctc ttgagcaatt tcaggaactt
     7201 gttcggcata agtaaccatt ccattttcgt caagaacaat aacaactctt gaatgtagac
     7261 cggctaatgg tccgtcaaca attgttaatc cataagcatc accaaaactt ccggttttaa
     7321 aatcggataa attttcaaca ttttctaatc cttcagcccc acaaaatcgt ttttgagcaa
     7381 atggtaaatc tctggaaata cataaaactt ttgtgttttc taaagaactc gctttttgat
     7441 tgaatgttcg gactgatgta gcacaaactc cggtatcaat actcggaaaa atatttaaaa
     7501 ctaatttgct tcctgcataa tcacttaaag atgtggaaga taaatctgtt tttattaaat
     7561 tgaaatcagg tgcttttgag cccgcttttg gtagttctcc ttgcgtatga ataggatttc
     7621 ctcctaaagt aattgatgcc ataattgtta tttaattagt gttcaaaagt atgaaaattt
     7681 tagtaatctt ttagtggatt ttcgcaacaa aaaaaacgcc acccgaagga agcgttttat
     7741 aaaggtattt gtttggaaat tatttgtcta ttgaacctaa aacccgtttc ataaacccgt
     7801 tcaaagcttc ttttttatcc attccgtttt gaatagattt ctgaacctct aacgctccat
     7861 acatattgga aattaattct ccaataacgt ccaactcttc ttcttttaag gatggaattt
     7921 ctgttaaagc ttctaaaact tcaatggttt ctacaagata atcttggtcg ttatcttcaa
     7981 taaactgggt taagtgtttt attaccggta gtttcattag tttgcagaaa tttcagaaac
     8041 caattcagct aatacctcgg ctttgttggt ttgtgtttga ttcaccaatt ctccgtttac
     8101 aaatgttgca aacgtgggca aattagaaac attagctaat tttcttgatt caggagaatt
     8161 ttcagcatct accaaaacaa acgtaatagc atcattttct gaagctaatt ttttgaattt
     8221 aggtttcatg attcggcaat ttccacacca agaagcagaa tattgaacga ctactttgtt
     8281 gttagttgtc actaaatctt gaagcgtatc ttcgtttaaa tcgattaaca tattttatgt
     8341 ttttaaaatt tcaatatcaa aaatcaaaaa ctgattcttt ttattgtgtt tgaaattgta
     8401 ttagttttga cttaaataag cagctgtgct taaacggtct gcgaccatag catctttacc
     8461 ttcttcccag ttagccggac atacttctcc tttagtttga acgtgtgtgt aagcatcgat
     8521 taaacgtaaa tattcattta catttcttcc taatggcata tcgtttacac tttcatggaa
     8581 aattttaccg gtttcatcaa ttaagtaagt agctctgtaa gtaacatttg atccgtcaat
     8641 taaataggaa tcagattcct cgttatattc accaacttca atatctaaga tgcctaaagc
     8701 cgatgataaa ttccgtgttg tatctgcaag aattggataa gttactcctt caattccgcc
     8761 attattttta gcggtattta accaagcaaa atgaacttcg tttgtgtcac aagaagcacc
     8821 aataacaacg gtatttcttt tttcaaattc cggtaaagca gtttgaaagg catgtaattc
     8881 tgttggacat acaaaagtga aatcttttgg gtaccaaaat aaaattactt ttttgttgtt
     8941 ttttacggct tcatcaagca cattgattct taaattatca cccatttcag aaatggcatc
     9001 tactgtaatg tttggaaatt ttttacctac taatgacata attattttta ttttaaatgt
     9061 taatttcagt tgcaaattta gtactttaca gaagagtaat ctatgatatt agtcattcct
     9121 ttttagtata gaagtattag ttttactgat gcttacgatg ataatggctt aaaattagtt
     9181 actctgtaat tttaagtaat tattaattca ttattcgtaa ttatcatact aaaacaatat
     9241 ggaatcaata gtagttaaag ttatattaaa taacaaaaac tgccgataaa agtagtttta
     9301 gataattttt gaaagtaaat cttatttagt tgttaactgc tttattttaa tattaaaggc
     9361 agcaaatagc aaagtcataa atggactgat tatattaaag aaagcatagg ggagatatga
     9421 aatagttgaa acgcctaaca ctccggattg ataggctcca caagtattcc aaggaattaa
     9481 tgcagatgta actgttccgg aatcttctaa agttctgctt aaattttcag gagctaaacc
     9541 tttatcttga taggctttag caaacatttt tccgggaact actattgcta aatactgatc
     9601 cgaagctgta aggtttaatg ctaaacaact ggcaacagta ctggcaaata agccaaaagt
     9661 agtgtggaac agttttaata aagaatctgt aattcgagtt aatgcaccaa tagcctccat
     9721 tacaccacca aataccatag cacaaagaat tagccaaatc gtactcatca ttcctttcat
     9781 ccctttagct gaaaataaat cagagagcat ttgattagtg gtttcaaccg aagtttcaac
     9841 ggtgatagca ttcataacac ctttatatcc ggaaacaaaa gataaatgat tacttccgcc
     9901 aatttttgct actaattccg gttggaaaat aagtgcaaaa acacctccta ataaggttcc
     9961 tattaataag gcaataagcg gttctgtttt tttgatgatt aaaattatga caattcccgg
    10021 aaccaaaaac aaccaaggtg tgatggtaaa ggcatctttg attgatgtta gcgttgctga
    10081 attatcacct gaaccattag ttgtttgagc taaacccaaa aaaataaaaa gtaataatgt
    10141 aataatcata gttggtacag tggtaattgt catataacga atgtgcgtaa acaaatctgt
    10201 tccagccata gccggtgcta aatttgttgt atcggacaat ggtgataatt tgtcaccaaa
    10261 ataagctccc gaaactacag ctccggcaac cattccaagc ggcattccga ttgttgaacc
    10321 aatgccaatc agagcaattc caactgttgc tgaagttgtc caagagcttc cagtcgcaat
    10381 tgaaattaca gaacaaatga ttaaacaagc cggaagaaaa atggcagggt gtaaaatttg
    10441 taaaccataa taaatcatag tcggaataac accactaatt aaccatgttc ctgaaagtgc
    10501 ccccaccatc aataatatta aaatggcccc tgcagtagat tttacgtttt tggcaatttc
    10561 ttccatcatg gcttgatagg aaactttatt tagaaaccca actattgccg caattgctcc
    10621 accgataatt aatgcaaatt gattcggacc agcagtagca ctgtcaccat aagcataaac
    10681 attataggct aaaatagcta ttaaaagtaa gataggaatt aatgcttcga aaatagaaag
    10741 ttccttattt tcaataagtt gttcgtcttt cgggtcttgt gatttaatat cttgactttc
    10801 catgtagttt gtttgtggat gcaatatact attttttttg aattttgaat tttaataaaa
    10861 aaatccgatt ctgagttgaa tcggattata tttacttaaa atgtattttt atttattcaa
    10921 aactcccagt tctatcatac acgctttcat cttttgataa gttcgttcga tatcattatc
    10981 caatccaatt gaaaatcgaa ttaatccgtc tgataagccc atttctttct gttcttcaat
    11041 tggaatttca gaggaagtag aagttcccgg tgcactgaac aatgttttgt aaaagcctaa
    11101 acttactgct aaataaccaa ggtttctgtt ttgcattaac tccattaatt cgttggcttt
    11161 gtccaatgag cccacatcca aagtcatcat gccaccaaat ccatattctt tatttatcat
    11221 tgaagcataa agttcataac tagggtgact ttttaatccg ggataaacta ctttcaaacc
    11281 atctttttca aatttttcag ctaaatagtg tgtattgtaa ctgtgttgtt tcattcggat
    11341 atgtaaggtt cttaagtttt ttaaaatact ggcagaacgt aaactatcca ttgttggccc
    11401 aaataacata ctcgcaccac tgtttacatt tttcaacgca ttgataaatt cttgagaagc
    11461 acaaaccact ccaccaactg tatcactgct tccattgata aattttgtta aactatggat
    11521 aacaatatct gcacctaatt gtgcaggagc aacggaaagt ggtgaaaagg tattgtcaac
    11581 cactaatttt aaattgtgct ttttagcaat tttggcgagc ccttcaatgt ctgctacttc
    11641 taataaaggg ttacttaccg tttcacaata tataaccttt gtattctgat taatagctgc
    11701 ctcgacaatg tctagtttag tgatgtctac aaaatgtgtt ttaatattcc atcgaggcat
    11761 catatttttc ataaaggcat aggttccacc ataaattgtt ctacttgaaa caatttcgtc
    11821 ttcggcattg cacaattgca ttaaaacagg ggtaatagca cccattccgg aagcagccac
    11881 attagcactt tccgttcctt ccatggccgc taaagctttg cctaaatata aattcattgg
    11941 agaagaatga cgcgaataca aatagcatcc ctcagcattt ccttcaaagg tgtcaaacat
    12001 tgttttagca gacaagaaag tataggttga actgtctgaa atggagggat tcacgcctcc
    12061 aaattctcca aaatattgta aatcttgaat ttgattggca gcattaaaat cagttggttt
    12121 cattttgatg ttttttaata tacattacaa aatacagaaa tattagaatt taaaacaata
    12181 tatagattaa aatttagatt atttaactat tatatttaat ttaaatagtt ttaatgacta
    12241 tatttgtctg ttgtttttta tttgaataga ttttttaaaa cctaactctt taattatgga
    12301 actcgataaa attgacaaaa aattactttc gttacttcaa acggatacca aacaaacaac
    12361 aaaagagctt tcactattgc tcaatttatc tgtaacagcg gtatatgaac gtataaaaaa
    12421 aatggaacgt ttaggcataa ttcaacaata tgttgctctt ttaaaccgtt caaaaattaa
    12481 caaaggattt gtggtttttt gccatgtcaa attggtgcaa catacccgag agtacatatc
    12541 cgactttgaa agtgaagtag tcaaactgca cgaagttttg gaatgttttc acgtgagtgg
    12601 tgattacgat tatattctga aaatatgtgt gggaaacatg gaagagtacc gtgaatttat
    12661 gataacaaaa ttaaccacgt taaaccacat tggcagtaca cacagttctt tcatgattgg
    12721 ggaggtaaaa aacacaaccg tttttgaatt ggtgtaaggc tttgaaattt taattttaac
    12781 gaattcttac caaaatctgc tctgaaaaat agtagatttg ttaatctaat taaattgcat
    12841 gaaaatagct atagtttgtt atcctacttt tggtgggagt ggagtagtag ccactgaatt
    12901 aggacttaaa ttggctcatc gtggacatga gatacatttt atcacgtacc gtcaacccgt
    12961 gcgattggct ttattgaatc caaatgtgca ttaccacgag gttaatgttc cggaataccc
    13021 gctttttcat tatcaacctt atgaattggc tctttccagt aaattagttg atatggttaa
    13081 gttgcataaa atcgaattaa tgcatgtgca ttatgctatc ccacacgcct atgccggtta
    13141 catggctaag caaatgttga aagatgaggg tatttatatt cctatggtta ctactttgca
    13201 tggaaccgat attactttag tagggaatca cccgttttat aaacctgccg ttagtttcag
    13261 tattaataaa tcggatgtag tgacttctgt ttctcaaagt ttgaaagatg aaacctacaa
    13321 gttgtttaat atcaccaaag aaattgaagt tattccaaat tttattgaat tggagaaaac
    13381 aaagttagag gaaggagctc cgtgttaccg ttctttgatg gcaacagatt cagaaaaaat
    13441 tatcacccat atcagtaatt ttcgaaaagt gaaacgcatt cctgatatca taaaaatatt
    13501 ccacaaaatc caacaacaaa ttccatcaaa attaatgatg gtaggggatg gaccggaaaa
    13561 agaaaaagcg gaattacttt gtgaagaatt agggatagca aataaagtta tcttttttgg
    13621 aaacagtaat gagattgata aaattttgtg tttttctgat ttgtttttat tgccttctga
    13681 gacggaaagt tttggattgg cagctttaga agccatggct tgtagtgttc cggtaatttc
    13741 aagtaatacc ggcggtttac cggaagtgaa tgtggacgga ttttcaggat atttaagcaa
    13801 tgtgggtgat atagattcga tggcggaaaa tgcagttcgt attttagaaa atgaaaccaa
    13861 cttagctagt ttcaagaaaa atgcttatac tgttgccgaa cgatttgata ttgcaaaaat
    13921 attaccactt tatgaagcaa tttatgaaaa agccattcaa gaacaattta aaacagaatc
    13981 aaactaagat gaaaaaaata attgccttct tgattataag tattgtattg ttttcttgtg
    14041 cctcaaaaac aaataatgcg gataaattac cactttatga aatcttaaaa caagaggatc
    14101 aaggtggagc aaattttaaa ttttttgaaa ttatttcaga accaaaagaa tttaaaatgc
    14161 ttttgaacga tgctgattta aagaaaaaaa taaaagcagc ggatatttat acgtgtaact
    14221 ttttattgtt aaacatgggg acaaaaagtt ccggaggata ttatttcact atcgaaaata
    14281 tagaagaaat ggaaaatgat attgtcataa ccatcaaaga aaatgaaccc aaaggaatgg
    14341 ctacatctgt aatgacaaat cctttggcaa tagtaaaaat aaattctaaa aaaaatatca
    14401 ttataaaata aaaactatcc taattaaaaa taccctcgat agtaagacac taagagggca
    14461 ttttaattta tattgattga attagaactg gtatcttata ctcaaagcga tgtcagaacc
    14521 ataattgtca tttctgtaat tatttccgaa ataaagttca ggtctgaaat ccaaagaaag
    14581 taacaacggt atatcaaaat tgtattcaat accaatatca ccggccatta ataaaaaggc
    14641 accatcttca tattcatgat ttttgtcatt gttagtccaa gttcctaatc ctgcaccaag
    14701 tcctgcatac cattgaaaac gtttttcaat tgtccaaacc cattggtgta aaccaacaat
    14761 tttaaaagca tcatcattgt cagaatttct taaaccaaga tcaaactcta aacgagtaga
    14821 attggataat tttctttgat aggatatttc agcaccaaaa ccatcattgc ttcctaaacg
    14881 taatcctaat gcattttttg aaatttcctg agcatctgca ctaaatgtaa acccaactaa
    14941 tataagggcc gataaaaata attttttcat agtatgtaaa ttttattact actgcaaagg
    15001 taaccccgaa aagagttaaa tcttgtaatt gcacgttaaa gtatgttaat tcaccgtgta
    15061 cgagtctaag aattcgtttt gagactgaga gcttgtttga ggaatgtcta aaccaaaatc
    15121 cgccggattg atggctaatg aataagaaat ttggttattt gaatagacca ttccaatatc
    15181 ttgagcataa taatgtgtag cagtcaaaac atcctgactg tttaaaatag gaattggtat
    15241 ggttatgttt gtacccggaa ttgtctgtat agtggtcact tctacattta taacaaagtt
    15301 aacttttttc acatcagtgt aagtcacatt tttagaagtt gtaaaactag gcagcgtttc
    15361 aagagctgta gatttaatgg tgtaatcaat agtaattgga gttccattta aatcttgaga
    15421 aattgtacct gattttgtag acattacttc attagaagta gcatcattat taattagaat
    15481 aaagtcttca agagcaaaat ccaacggaat atcaacgcct aaatctaagg caagtgtgcc
    15541 ggttaagagt aacttttggc cctcatttct aaggccgtta tctctcaaaa gcgacgaata
    15601 aaatccaaaa ggaacatccg tcgtcttcat ttgttggtag gatttattgt ttaaaattat
    15661 ttcatctcca acatataaat tatcaaaatc tgtggtttgt ccttctactt cataggtcca
    15721 actattgtcc gatgttaagg ggaaatagtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn tttgtccttc tacttcatag gtccaactat tgtccgatgt taaggggaaa
    15901 tagttgaaat aggcatcacc ggaatcatcg gaggaacaag atgaaagagt tagcattaga
    15961 acagtcagta gtaaaaaatt tgtcttcata attaggttta attaattgtg gaacaaatta
    16021 aagcaaaaga aatcattttt gcgaaaaaat taacgaagcg ataagttgct cacttaattt
    16081 tgattccatt tcaaaaaaat cttcttcaat catgttggtg tctttgtcat aacgatacaa
    16141 tttccaacgc tttttattgt attccaatgc tgttaagttt tcaacccaat cacccgaatt
    16201 taaataaaaa gtggtcccct ttttagtaac atttcgaatc atttttggtt cgtggatatg
    16261 accgcaaatc acataatcgt aattattttt aatggctaat tcagatgctg tcaattcaaa
    16321 atctgaaata tgtttgaccg ctttttttac attcgctttt atttttttgg aaaacgagta
    16381 gggttctttc cccatttttt ctaatattcg gttgacaaat cgatttacta aaatcagtaa
    16441 atcatatccc catcctccta atttagctat ccatttggaa tgttgtaccg aggcatcgaa
    16501 aacatcacca tgaaaaatcc atgctttttt gtcatctaat tccaaaacta atttgtccaa
    16561 aattgaaaaa tttccaatct tcgtatcact aaattttcgc aacatttcat catgattgcc
    16621 ggtgatataa taaacttcgg ttcctttcga agccatgtct attatttttt taattacttt
    16681 taagtgtgct ttcgggaaat aggattttct aaattgccag atatctataa tatcaccatt
    16741 caaaacgagt atttttggct ttatagttga taaatagtgc actaattgtt tagcatgaca
    16801 accaaaagtt cctaaatgta catcggaaat aacaacaact tctacttttc tttttttaag
    16861 cattgtgatg gaaggttttg caaattaacc atctaaattc aaaatgcatt ttacttaaaa
    16921 gttaacgttt ggtcaaccaa tattattttt gtgtgaagtc aaaccgcttt tatgagtatt
    16981 ctttaaatta aaaaacttat ttttgttcaa aacattccta ataatggcag gaaacagtta
    17041 cggaacctta tttaaactta gcacttttgg cgaatctcat ggtgatgctt taggaggtat
    17101 tattgatggt tgtccacccg gaattcatct tgattttgaa gcgattcaat tcgaaatgag
    17161 ccgacgtaaa cccgggcaat caaacattgt tacccaacga aaagaagccg atgaagtaca
    17221 gtttttatcc gggatttttg aaggtgttac aaccggaaca ccaattgggt ttattattcc
    17281 taattccaat caaaagccaa ctgattatgc acatttaaaa gaagcttttc gacccagtca
    17341 tgctgatttt acgtatgaaa agaaatatgg cattcgcgat tatcgtggcg gtggaagaag
    17401 ttctgccaga gaaacaactt gtcgggtagt agcgggagcg attgcaaaac aagtaatttc
    17461 atccattaaa attaatgctt ttgtttcctc ggttggttca ctgtttattg acaaacctta
    17521 tcaggaactt gacttttcta aaattgaaaa taatcctgtt cgttgtcccg atgaaaagac
    17581 agctgctctt atggaagatt acatcaaaga aatcagaaag caaggagata cggttggagg
    17641 aaccattacc tgtgtcattc aaaatgctcc aattggttta ggggaacctg tttttgataa
    17701 attgcatgcc gaactaggaa aagcgatgct ttccatcaat gcggtgaagg gttttgaatt
    17761 tggcagtgga ttttgtggag ctcaaatgaa aggcagcgaa cacaatgatt tattcaatgc
    17821 agatggaaca acaaaatcaa atttgtccgg aggaattcaa ggaggtattt ctaacggaat
    17881 ggatatctat tttcgggttg cttttaaacc ggttgccacc attatgcaaa aacaagaaac
    17941 aattgataac aaaggaaaga ttgtcgaatt ggaaggcaaa ggaagacatg atccttgtgt
    18001 tgtgcctaga gccgttccca ttgtagaagc catggccgct ttagttttgg ccgacttttt
    18061 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagagccgt tcccattgta
    18181 gaagccatgg ccgctttagt tttggccgac ttttttttac taaataaaac ctataaataa
    18241 ttctatatga aattagctct tcactggaaa attctaatcg gaatggtgtt tggtttagct
    18301 ttcggatttg taatgaaaaa tctcgaaatg aaaggcattg tggtcgattg gataaaaccg
    18361 tttggtacaa tttttatcaa cttactaaaa atgattgcgg ttccgttaat cattgtttct
    18421 ttaatcgttg gattatctga tttgaaagat atttctaaac tttctaaatt gggtggtaga
    18481 accgttgttt tatatttagt tactaccgtc accgcagtat ctatcggatt agttttagct
    18541 aatttgataa aaccgggtga atatatcaat gatgaatcac gtaaaagttt attagaaaat
    18601 tttgccggtg atgctgcaaa aaagattgaa ctggctgcga gtgctaaaaa tcaaggccct
    18661 cttcaaccct tagtggatgt tgttcctgag aattttttct tagccatgac agataatgct
    18721 tctatgttga aagtaatttt atttgtcatt ttaattggaa ttggtttgat tttaattgat
    18781 gaaaataaag ctaaaccagt agtcgatttt ttcaaaggat tgaatgaagt tattctaaaa
    18841 attattgata ttatcatgct tttttctccc attggtgttt ttgctctaat ggctgcttta
    18901 atggttgaaa tccctgattt ttcaacgtta aatgcgttag gaatttatgc tttgacggtc
    18961 gttttaggat taataattat gacttttgta atatacccaa caatgttgat tgtattggca
    19021 aaatattcgc ctgtgaaatt ttttagagct atagcaccgg cacaattatt agctttttct
    19081 acgagttcca gtgcggctac tttgccggta acgatggagc gggtaataga aaatgtaggg
    19141 gtagatgagg aagtggccag ttttgtactt cctttaggtg ctacggttaa tatggatgga
    19201 acaagtttat accaagcagt cgctgccgtt tttattgctc aggcaatggg aattccgtta
    19261 gatttacaaa cccaattgat gattatcgtt actgcaacat tagcttccat tggttctgca
    19321 gcggtgccta gtgcaggaat ggtgatgttg gtgattgtct taggacaagc cggaattccg
    19381 gaagccggtt tagccttaat atttgctatt gatagaccgc tagacatgtg tagaaccgtt
    19441 gtgaatgtaa ctagtgatgc tgttgtttct acggttgttg ctaaatctgt tggaaaatta
    19501 aaccccatta acacagaagt ttaatttttt ttcttatttt cgt
//
LOCUS       DAOTCW010000035        19534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1516_length_19534_cov_44.971251, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000035 DAOTCW010000000
VERSION     DAOTCW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 19534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19534
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1516_length_19534_cov_44.971251"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..645
                     /locus_tag="P5335_04535"
     CDS             61..645
                     /locus_tag="P5335_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRZ74179.1"
                     /translation="MKKLILSVVALLIFGFAQAQREGGFRVGFDLGIIPASGGLGVSF
                     DIEPKYNIKENMSVGLRLGGAAIARDVEEIGNTTLVTATAIGSYIGTFDYYFPSGKSF
                     VPFVGGGIGYATALSVRADEDANIDDLDPDSGFCGVIRGGFEWGKFRMNLEYNFVPKT
                     PLYLNGPLTTEDISNSYFAVNLGFYIGGGKWKKN"
     gene            724..1620
                     /gene="xerD"
                     /locus_tag="P5335_04540"
     CDS             724..1620
                     /gene="xerD"
                     /locus_tag="P5335_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRZ74180.1"
                     /translation="MNWQTYIKSFQSYLKIERGLSQNTLVNYTFDLVRLTQFLEANGI
                     SKNPINISEDEIQLFIYEVSKEVNPRSQARIISGLKSFFTYLIFEDYRTDNPMELIET
                     PKIGRKLPDTLSIQDIDSLIAAIDLSKEEGERNKAMLETLYGCGLRVSELTTLKISDL
                     FFEEGFIKITGKGNKQRFVPIGKSTEKYINLYKNHVRSHLTIQKGHEDTLFLNRRGKQ
                     LTRAMIFTIIKDLAVKINLNKSISPHTFRHSFATHLLENGADLRSIQLMLGHESITTT
                     EVYVHLDRKHLSQIVNKFHPRK"
     gene            complement(1710..3275)
                     /gene="rny"
                     /locus_tag="P5335_04545"
     CDS             complement(1710..3275)
                     /gene="rny"
                     /locus_tag="P5335_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRZ74181.1"
                     /translation="MDSTLLLIIAGIVGIAAGFAIAKVIEKSNVSNLIKNAKKEAASI
                     LKDAKVEAENIKKDKLLQAKEKFLELKSEHEKEILSKDKKIAEVEKRTRDKESQVSSE
                     LAKAKKSADDFDIKIADYNNRIELLDKKQIELEKLHKSQVQQLEVISGLSAEEAKEQL
                     VESLKAEAKTKAMAHIQDTLEEAKLTAQQEAKKVIINTIQRVGTEEAVENCVSVFNIE
                     SDDVKGRIIGREGRNIRALEAATGVEIIVDDTPEAIILSCFDPVRREIARLALHKLVT
                     DGRIHPARIEEVVAKTTKQIDDEIIEVGKRTVIDLGIHGLHPELIKVVGRMKYRSSYG
                     QNLLQHSREVSKLCGIMAAELGLNVKLAKRAGLLHDIGKVPDTESELPHAILGMQWAE
                     KYGEKEEVCNAIGAHHDEIEMKYLISPIIQVCDAISGARPGARRQVLDSYIQRLKDLE
                     AIAFGFNGVKSAYAIQAGRELRVIVESEKVSDEQAAKLSFEVSQKIQTEMTYPGQVKI
                     TVIRETRAVNIAK"
     gene            complement(3324..3432)
                     /gene="ssrS"
                     /locus_tag="P5335_04550"
     ncRNA           complement(3324..3432)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="P5335_04550"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01685"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="flavobacterial type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01685"
     gene            complement(3487..3780)
                     /locus_tag="P5335_04555"
     CDS             complement(3487..3780)
                     /locus_tag="P5335_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRZ74182.1"
                     /translation="MNEKLKIKLSIADRVYPLTVDPSQEEGLRSASKKIDVMIKQFEQ
                     NYAVRDKQDVLAMCALQFASQLEQKQIEKTEDNQESLERLKKINDLLANYLEK"
     gene            complement(3787..4077)
                     /locus_tag="P5335_04560"
     CDS             complement(3787..4077)
                     /locus_tag="P5335_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74183.1"
                     /translation="MSEMVEIVDSLENRLSKLISKMQGLETTIQDLKQELNKANRNIQ
                     IQASEMESLKKQYETLKIANSLLGSDENKRDTKLKINSLIREIDYCIAQLSD"
     gene            4264..5961
                     /locus_tag="P5335_04565"
     CDS             4264..5961
                     /locus_tag="P5335_04565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRZ74184.1"
                     /translation="MKYTLLLLFASINAFSQVNYPQNDFISPLDIPLFLSGTFGELRS
                     NHFHSGLDFRTQSKTGFNVYATGDGYISRIKISTFGYGKALYITHPNGYTSVYGHLLK
                     TAPKIDAYLKEQHYKEQSFEVDLFLGEGVLPVKQGEIIALSGNSGGSGGPHLHFEYRD
                     SQTEKIINPLFFGLDAKIKDTKVPVVNEIVVYPISDDAVVNQSQQPISIPLSLQKDGT
                     YLASKVLAKGKIGIGVNTYDGSDNNYGKNGIYKMQTYLDGKPAFDIAFNSFSYDETRY
                     INAYIDYYRYKKMNQRVQKMYYTKKYPLSLISSSLTDGIITIPPNITSIYKVEIADFN
                     GNKTAVTIPIESANHEVKIPKAIKKTNYFIKSDNEYIFEKDKISVFIPEQTFYDDFHL
                     KFEVKDKVLYLHDDSTPVHSNFSVTFEEDSIPDADRDKFFIATINGKKTYYNSTKMKE
                     KKFTTWTRNLGEFRLIKDSISPKITSVNFQEGKWLSKQNDLQFEITDDLSGIKTYTGY
                     LNGKWILFDYDYKTNKITHDFSDNVVIEGRNELKIVVTDNVGNSTIFESHFFRSQQP"
     gene            5971..8436
                     /locus_tag="P5335_04570"
     CDS             5971..8436
                     /locus_tag="P5335_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ74185.1"
                     /translation="MKTKQILLAFLLFCVGFASNAQTARIKGIILDKNNNPVEGVSIN
                     FQDKGTVTNENGFYILTIPANQRITLVFTHISLKRATLRIELKTNEDYEYNFQMSDSD
                     ELLGEVIITSGKQKRIEGITAIEPAIIRQIPGANAGVENILKTLPGVNSNNELSTQYS
                     VRGGNYDENLVYVNEIEVYRPFLIRSGQQEGLSFTNTDMVQNVDFSAGGFQAKYGDKL
                     SSVLDITYRIPTHFAAALEASLLGGSLTVEGVSKNQKWANITGVRYRDNSLLVNSQET
                     ESNYKPTFADIQTYLTYTPNARWQWGFLGNISQNTYNYEPLTRQTNFGTIDEPIALQV
                     FYEGQEKDKYQTLFGAIKTSFTVNDNTTLKFIGSVYNTQEQEYFDILAAYYLGEVDSN
                     IGSETFGDVTFNRGIGTQLNHGRNNLDALIINAEVKGFHKIETHAIEWGVKYTREDIR
                     DRLVEWEVIDSAGFSINPPRDFPVNDQPYNPYTGPLVPYQDVRATNFVTIDRFSGYGQ
                     WSKKASLGTSEVWYNAGVRMQHWQVSGDGVKSQTQTVFSPRAQFAIKPDWNSDMVFRV
                     SGGFYYQPPFYRELRDYEGVIQPDVKAQQSIHFIMGNDYSFKMWNRPFKLVSELYYKT
                     LADVNAYTVDNVRIRYRADNETEAYVYGADFRINGEFVPGTESWFSLGYLKTEENIQD
                     KGYIARPSDQRLKFGVLFQDYVPSIPSLKMYLNLVYNTGLPGGSPSYADPYDYQLRLK
                     DYRRADVGFSYVLTEKNEIRPEGHWLKRFKDLSVGLEIFNLFNNQNAITNTWVRDVYT
                     KTQYGIPNYMTTRVFNVKLTARL"
     gene            8481..9206
                     /locus_tag="P5335_04575"
     CDS             8481..9206
                     /locus_tag="P5335_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74186.1"
                     /translation="MKTFINAVLLLILVVTFGCKEDEKEKPKVTYKESPAQVEVKKDT
                     TQIKIADLPIHMEGTSFLLHPIGDVRVYDGSSKSSYGSSSTSRVSYSISNYNRFEITG
                     YLNNIRFQHLDSTATRPLTDKIVIIQTANFLNTIAEKTKKQILVYSISDMDTNKDGKL
                     DANDIKSLYISEISGNHFMKLSPDFQELIDWNLIEAKNRLYFRTTEDTNKNGDFDKDD
                     TVFYYFVDLMDKEWKLNEYKPFH"
     gene            complement(9203..9904)
                     /locus_tag="P5335_04580"
     CDS             complement(9203..9904)
                     /locus_tag="P5335_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HRZ74187.1"
                     /translation="MYIHNTDIILPENPNTIVWKYLDLSKFLDLLLSKKLFMSRSDKF
                     EDQYEGTFSEPTYEEIRKIAENNPEFLDYYKSHREKIVISSWHINEYESFAMWQIFTK
                     NNEGLAIQSTVKRLQEAMKPETKTTQYIGEVNYIDYKKEYIPFDDTFFPFLFKRKSFQ
                     YEREVRIISDVTQNNIVINEGLKIDVDIHQLIEKIYIHPKSENWYKNLVIQLMEQLGF
                     NFSIEKSDLESDILI"
     gene            complement(9960..11807)
                     /locus_tag="P5335_04585"
     CDS             complement(9960..11807)
                     /locus_tag="P5335_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRZ74188.1"
                     /translation="MDENLKKIIPFLKPYQSNVVLNIIFNILYALFSTLSFITLIPML
                     QVLFENAEKVATKPTYQSIWEITSYGKDYLYYYITKLSDDNGPQYALLLVVSLVISTF
                     LLKNFFNYLALNHLMLIKNGVLRDLRNKMFDKIIDLPISYYSEKRKGDVMARMLNDVN
                     EVKNSFFSILELVVKEPMTILFTIGAMVAISFKLTLFVFIFIPVSGFIITKIGKSLKS
                     KSKRAQDENGYLISIVEETLAGLKVVKSYNSEENFRSKFKNSIQRLFKLSNSIGRKNN
                     LSSPLSEFMGIVVIAILLWYGGNMVLVGKLADGSPILEGAHFLAYMGLAYNILTPAKA
                     ISKASYQVKTGLAAAERVFDVLEVENSIVDKPNALQKTSFDESISIEDINFKYEEENV
                     LKNFSLKIPKGKTVALVGQSGSGKSTIANLLTRFYDVQDGCVKIDGIDIKDISLESLR
                     NLMGLVTQDSILFNDTIKNNILIGKQNASDTEIIEALKIANAFEFVNDLPLGIETNIG
                     DSGGKLSGGQKQRLSIARAVLKNPPIMILDEATSALDTESEKFVQVALENMMQNRTSI
                     VIAHRLSTIQKADLIVVMQKGEIVEQGTHDELLSQNGMYSKLVMMQSFE"
     gene            complement(11807..13534)
                     /locus_tag="P5335_04590"
     CDS             complement(11807..13534)
                     /locus_tag="P5335_04590"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006261825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRZ74189.1"
                     /translation="MHIPQNILDKVNVWLTPTFDLQTREEIEKMMTSSPKELEESFYK
                     NLEFGTGGMRGIMGVGTNRINKYTLGKNTQGISNYLKHSFPKEALKVVIAYDCRHNSD
                     TLAKVVADVFSANGIKVFLFSDMRPTPELSFALKHLNCHAGIVLTASHNPPEYNGYKV
                     YWQDGGQLVPPQDEEIIEVIEALAYDEIKFTANESLIEYIDINIDEAFFTSTIENASF
                     NTPKTAKENLKIVYTSLHGTSIKAIPAVLAKAGYTDVNIVKEQAQPDGNFPTVKSPNP
                     EEPEALTMALALADKINADIVIGTDPDSDRLGVAVRDTEGKMKLLNGNQTMVIMTAFL
                     LEQWKREERFKGNEFIGSTIVSTPMMLELASAYGVECKVGLTGFKWIAKFIKDFPNQK
                     FVGGGEESFGYMVGDAVRDKDAVAATLLVCEIAAQAKSAGSTLYKELLNLYVDFGFYK
                     EYLISITKKGIEGANEIKQMMVDLRENPLKEINGQRVVCIEDYDNSTARNLMTEEIEL
                     LEIPKSNVLIYYLEDGTKICARPSGTEPKIKFYFSVNNSLDSIEQFKNVEAILDQKIA
                     TIIAEMHLN"
     gene            complement(13539..14030)
                     /locus_tag="P5335_04595"
     CDS             complement(13539..14030)
                     /locus_tag="P5335_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HRZ74190.1"
                     /translation="MKLKIIQQQIIELRGQKVMLDFHLAELYEIETKVLKQAVRRNIN
                     RFPPDFMFELTQNEFESLRSQFVTSKRGGLRYMPFAFTEQGIAMLSSVLNSEKAIAIN
                     IAIMRTFVSLRQFALNYKELNDKITEIEKQFPDIYNILNYLMDKEKTATPKIERNKIG
                     FKK"
     gene            complement(14070..15029)
                     /locus_tag="P5335_04600"
     CDS             complement(14070..15029)
                     /locus_tag="P5335_04600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRZ74191.1"
                     /translation="MNLSIVIPLLNEEDSLRELHDWVVTVMNKSNFTYEIIFIDDGST
                     DNSWKVIEQLSNENPNVKAIKFLKNFGKSQALHAGFNKAQGDVVITMDADLQDNPEEI
                     PELYELITSQNFDLVSGWKKKRYDSVMSKNLPSKLFNWAARKTSGVQLNDFNCGLKAY
                     KNKVIKNIEVSGEMHRYIPVLAKNAGFSKIGEKVVKHQARKYGETKFGMERFINGFLD
                     LITIWFLSRFGKRPMHLFGAWGVMMFLIGFTATGYIGISKLYRLYNDLPYDLVTNSPY
                     FYIALTTMIIGSQLFLAGFLGEIILRNKESNENYKISNELNMN"
     gene            complement(15029..15559)
                     /locus_tag="P5335_04605"
     CDS             complement(15029..15559)
                     /locus_tag="P5335_04605"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRZ74192.1"
                     /translation="MENSIKKNGFTLGIIMGVILVLITTTMYTVDLSLFTNWWVGIVN
                     FVIIIGIAIYCSITNKKALKGQMSYKEAFLSFILPIIVGIAIYVLYNIILFNVIDPEA
                     KNVLTENVITMTKEMMGKFNVPAAEINKAIADIESKDNFGPLSQFQSYFFQIAFYAVL
                     GLLVALIFKTPTSAKE"
     gene            15797..16048
                     /locus_tag="P5335_04610"
     CDS             15797..16048
                     /locus_tag="P5335_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRZ74193.1"
                     /translation="MKKGIHPENYRLVAFKDMSNEDVFITKSTVETKETITHEGVEYP
                     VFKMEISRTSHPFYTGKSKLIDTAGRIDKFKNKYANRAK"
     gene            complement(16120..16653)
                     /locus_tag="P5335_04615"
     CDS             complement(16120..16653)
                     /locus_tag="P5335_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HRZ74194.1"
                     /translation="MKKIIASLMLVSLIACEQNPTLQKYFVENTESSDFISVDVSPSI
                     LNIKPDALTGEQQEALKTFKKMNVLAFKANEQNKVKFDTERTKVTEILKDEKYQQLMK
                     FGSGKEGAAVYFVGKDDAIEEFVLYGNKNENGFAVVRILGDKMNPEDFMTLFSALQKS
                     DIGMEQLKPLEDLFKNN"
     gene            complement(16654..17868)
                     /locus_tag="P5335_04620"
     CDS             complement(16654..17868)
                     /locus_tag="P5335_04620"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRZ74195.1"
                     /translation="MRKIVLFFFSLSLFTVNAQSTVFEGKIVDKSTKESIPFANVIAL
                     QTAQGVISNEDGVFKFYISKNVTTIEISSLGYQSKKFEVAKIDSTFTTVFLEIDEIAL
                     EEVIVTNKPVATILSGIISHSKTLLDKSVKLETYYREFVKINNKYSKFADGLIDFYLQ
                     PKKKDKLEAKLIVNQSRAYQLIGADEIEKKGKADLAEMDSFFDIQKAANGFFSYDYIE
                     KSILSKKNEPNYDFELRSKKDQYGNAIEVIYIIPKPNVDEVLLEGKITYDPVNRIVLD
                     IDIKMSEKHKKNVEITNMLLFKYAFYDIQIKQSYKYVNGKYIPSYKKTLLDVYIKFGG
                     QMNDRLMSISDLVVTNFEDTIITSPDKNTTYQERSLYPNGMNYKENFWETNNAMPLSE
                     NEERILKTLKNN"
     gene            complement(17970..18503)
                     /locus_tag="P5335_04625"
     CDS             complement(17970..18503)
                     /locus_tag="P5335_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HRZ74196.1"
                     /translation="MIKFVTTLVVFLVSTIMFGQSVFDKFDGQEEVSTIIVNKKMFEM
                     MSKVKMDPADKEAQQYLNLIKKLDNLKVFVTSNSKITADMRTTADKYLKTAGLEELMR
                     VSDGGKNVRIYMKSGAKDTEVRELFMFMEGGPKDKETVLLSLTGNFDLNEISALTEKM
                     NLPGGDDLKKVSTKGKK"
     gene            complement(18509..18967)
                     /locus_tag="P5335_04630"
     CDS             complement(18509..18967)
                     /locus_tag="P5335_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74197.1"
                     /translation="MELARIEELIEKYFQGDTTLAEEKQLKQYFSTETVAAHLEHYKP
                     LFNYLTVNDKELFNKELSLPSKKRFSVAWIAIAASLVFFGGLFAYLNYKPSPAPAQVV
                     SDLGSFDSPEEAFEETQKALALLSENVNEGVESIAYLNEYEKSKQLIFKK"
     gene            complement(18954..19463)
                     /locus_tag="P5335_04635"
     CDS             complement(18954..19463)
                     /locus_tag="P5335_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRZ74198.1"
                     /translation="MNQNEFIHLIEPFKDKVFRLAKRLLVSSEEAQDATQDILVKLWD
                     KKNGLGQYKNVEALAMTMTKNYCLDQLKSKRASNLKIVHTNFTDREAGLDQKMEDQDS
                     WSWVEKIINELPEQQKLIIQMRDIEQYDFEEIAKMLDMNETAIRVALSRARKTIREKM
                     TKTHQYGIS"
BASE COUNT         6152 a         3261 c         3160 g         6961 t
ORIGIN      
        1 tttttgtttt atagacagaa ttttattctt aaatttgtgt aaacaaaaat aatataaaat
       61 atgaaaaaat taatcttatc ggtcgtagcc ttgttaattt tcgggtttgc tcaagcacaa
      121 cgcgagggag gctttcgggt tggttttgat ttaggaatta ttcctgccag tgggggatta
      181 ggtgtttcat ttgatattga accaaaatac aatattaaag aaaatatgag tgttggttta
      241 cgtttaggtg gtgctgccat tgctcgtgac gtggaagaga ttggtaatac aacattagta
      301 acagccactg caattggttc ctatattgga acttttgatt actattttcc atcgggtaaa
      361 tcttttgtac cgtttgtagg cggtggtata ggttatgcaa ctgctttaag tgttcgtgca
      421 gatgaggatg caaacataga tgatttggac ccggatagtg gtttttgtgg agtaattagg
      481 ggtggatttg aatggggaaa attcagaatg aatttagagt acaactttgt acctaaaaca
      541 ccgctttatt tgaatgggcc attaaccact gaagatattt ctaattctta ttttgctgta
      601 aaccttggtt tttatatagg tggaggaaaa tggaaaaaaa attaacttta attagtttac
      661 tattaaaacc tttcaatttc tgaaaggttt ttttatttcc ttaaatcaga ttatatttga
      721 aacatgaact ggcaaacgta tataaaaagt tttcaatctt atttaaaaat agaaagggga
      781 ttgtctcaaa atacgttagt caattatact tttgatttag ttcgtctgac acaattttta
      841 gaagctaacg gaatttctaa aaatcctata aatatcagtg aagatgaaat tcaacttttt
      901 atttatgaag tttctaaaga ggttaatcca cgatcccagg cccgaattat ttccggatta
      961 aaaagttttt ttacctattt aatttttgaa gattaccgaa cggataatcc gatggaatta
     1021 attgaaacgc caaaaatcgg cagaaaatta ccggacacac tttccattca ggatattgat
     1081 tcgttgattg ctgccatcga tttatcaaaa gaagaggggg aacgaaataa ggcaatgctg
     1141 gaaacgttat acggttgtgg gttacgtgtt tcggaattga caaccttaaa aatttctgac
     1201 ttgttttttg aggaagggtt tatcaaaatc accggaaaag ggaacaagca acgttttgtt
     1261 cccattggaa aatctaccga aaagtacatc aatctatata aaaatcatgt cagatcacat
     1321 ttgacgattc aaaaaggaca tgaagacaca ttgtttctaa accgaagagg taagcagttg
     1381 acaagggcaa tgatttttac tattatcaaa gatttagccg ttaaaatcaa tttgaataaa
     1441 tcaatcagtc cacatacatt caggcattca tttgccacgc atcttttaga aaacggtgcc
     1501 gatttgcgtt ccattcagtt gatgttgggt catgaatcga ttactactac cgaagtttat
     1561 gttcacttgg atcgtaaaca tttatcacaa attgtaaata aatttcatcc gaggaaatag
     1621 ttttcagtct agtttagata taaaaaaatg acttacattt ctgcaagtca ttttctttag
     1681 aatttatttt ttttaactga caaaagtgtc tacttcgcaa tatttactgc tctggtttca
     1741 cgaatcacgg taatttttac ctgtccggga taagtcattt ctgtttgaat tttttgagaa
     1801 acttcaaagg aaagtttggc ggcttgttca tcagaaactt tttcactttc cacaattaca
     1861 cgtaattcac gaccggcctg aatggcatag gcacttttca ccccattaaa tccaaacgcg
     1921 attgcttcta aatctttcaa acgttgaata taagaatcta aaacttgacg tcttgctccc
     1981 ggtcttgccc ctgaaatagc atcacaaact tggataatag gagaaattaa atacttcatt
     2041 tcaatttcat cgtggtgagc tccaatggcg ttacacactt cttctttctc accatatttt
     2101 tctgcccatt gcatacctaa aatagcatga ggtaattcac tttctgtatc aggtactttt
     2161 ccgatgtcgt gcagtaaacc ggcacgtttt gcaagtttca cgtttaagcc taattcagcc
     2221 gccatgatac cacaaagttt agaaacttca cgcgagtgtt gcaataagtt ttgtccgtag
     2281 gaagaacggt atttcattct acccaccact ttaatcaatt ccgggtgtaa accatgtatt
     2341 cctaaatcga taaccgtacg tttacctact tcaataattt catcgtcaat ttgtttagta
     2401 gttttagcaa caacttcttc aattcgagca gggtgaatac gaccgtcggt aaccaattta
     2461 tgtaatgcta aacgggcaat ttctcttcga acaggatcaa aacaagaaag tataattgct
     2521 tccggggtat catcaacaat aatttcaact cctgttgcgg cttctaatgc acgaatattt
     2581 cgtccttcac gaccaatgat tcttcctttt acgtcatctg attcaatgtt gaaaacagac
     2641 acacaatttt caactgcttc ttcagttcct acccgttgaa tcgtattgat gattactttt
     2701 ttagcttctt gttgagctgt taatttggct tcctccaagg tgtcttgaat atgagccatt
     2761 gctttagttt tagcttctgc ttttaagctt tctactaatt gttcttttgc ttcttcagca
     2821 gataaaccgg aaataacttc taattgttgc acttggcttt tatgaagttt ttccagttca
     2881 atttgttttt tgtctaaaag ttcaattcgg ttattgtaat ctgcaatttt gatatcaaaa
     2941 tcatctgctg atttctttgc tttagctagt tcactcgaaa cttgagattc tttatctctt
     3001 gttctttttt caacttccgc tatttttttg tctttggata aaatctcttt ttcatgttct
     3061 gattttaact ctaaaaattt ttctttcgct tgaaggagtt tgtctttttt gatattttca
     3121 gcttcaactt ttgcatcttt taagatggaa gcggcttcct tttttgcgtt ttttataagg
     3181 ttggaaacgt tgcttttttc aatgactttt gcgattgcaa aacccgctgc aattccaaca
     3241 attccggcga taataagtag taatgtactg tccatttgtt ttaaggttta tatataaaaa
     3301 aagcctacat taggtgattg tataaactcg ataagacaag ttttgagcta actcattgtt
     3361 caaggatccc actacaagga gggcatgctt tagtaatgaa gatttgctca ttctaaattg
     3421 ttagtgttga gtttatcaaa tagaaactaa tgtaggcagt atcttaatta gtgtgtaaga
     3481 acgtatttat ttttccagat agttggctaa taaatcattt atttttttca gtctctctaa
     3541 cgattcctga ttatcttccg ttttctcaat ttgtttttgc tctaattgcg aggcaaactg
     3601 taaggcacac atggccaaaa catcttgttt gtctctcaca gcataatttt gttcaaattg
     3661 cttaatcatt acatcaattt tttttgaagc acttcgcaat ccttcttcct gacttgggtc
     3721 caccgttaac ggataaacgc ggtcagcaat tgataattta attttaagct tttcgttcat
     3781 gctgtattaa tcagataact gtgcaataca gtaatcaatt tcacgaatta atgaatttat
     3841 tttaagcttc gtatctcttt tgttttcgtc actgcctaat aatgaattgg ctattttgag
     3901 tgtttcatac tgttttttca agctttccat ctcactggct tgaatttgta tgtttcggtt
     3961 tgctttgttt aattcctgtt ttaaatcttg tatagttgtc tctaaacctt gcattttgct
     4021 tatcagtttg gagagtctat tttcaagaga atcaacaatt tctaccattt cactcattgg
     4081 agcatatatt cattacacga tttacaaagt tagtattcct aaacgattaa cgcaaataaa
     4141 aaaaataaat tttatattca atttttattt ctcactaact gatggtaaat cattgaaata
     4201 gtatttttta gctttggttt gaaatttgct atactttttt taaattagca aaaattcgaa
     4261 gccatgaaat acactttatt attacttttt gcctcaatca atgccttttc acaggtgaac
     4321 tatccacaaa atgattttat ttctccttta gatattcctt tgtttctttc cggcactttc
     4381 ggtgaattgc gttctaatca ttttcattcc ggtttagatt ttagaacaca atcaaaaact
     4441 ggttttaatg tttatgcaac cggagatggg tatatatcca gaattaaaat ttccactttt
     4501 ggttatggta aagcacttta tattacccat ccaaacgggt atacttccgt ttatggacat
     4561 ttgttgaaga ctgcccctaa gattgacgcc tatttaaaag agcaacatta taaagaacaa
     4621 agtttcgaag ttgacctttt tttgggcgaa ggtgttctac cggtgaagca gggagagata
     4681 attgctttgt ccggaaattc aggaggttcc ggcggtccgc atttgcattt tgaataccga
     4741 gattcacaaa ctgaaaaaat aatcaatcca ttgttttttg gattagatgc taaaataaaa
     4801 gacactaaag ttccggttgt gaacgaaata gtggtttatc ccatcagtga cgatgctgtt
     4861 gtgaatcaat ctcaacagcc gatttctatt cccttatcat tacaaaaaga cggaacttat
     4921 ttagcctcaa aagttttagc taaaggtaaa atcggtattg gggttaatac ctatgacgga
     4981 tcggataata attacggtaa aaacggaatt tataaaatgc aaacatattt agatggaaaa
     5041 ccggcttttg atattgcttt taattcattt agctatgatg aaacccgtta tattaatgcg
     5101 tatattgatt attatcggta taaaaagatg aatcaacggg ttcagaaaat gtattatacc
     5161 aaaaaatatc ccctcagttt aatttcttcc agtttaacgg atggaattat tacaattccg
     5221 cctaatatta catctattta taaagttgaa atagcggatt ttaatggcaa taaaacagca
     5281 gtaactattc cgatagagtc tgccaatcat gaggttaaaa ttccaaaagc aataaaaaaa
     5341 acgaattatt ttattaaatc cgataacgaa tatatttttg aaaaagataa gatttcagta
     5401 tttatacctg agcaaacttt ttacgatgat tttcatttaa aatttgaagt aaaagacaag
     5461 gttttgtatt tgcacgatga ttcaacgccc gttcattcca atttttctgt cacttttgaa
     5521 gaggattcga ttcctgacgc tgaccgtgat aagtttttca ttgccaccat taacggtaaa
     5581 aaaacatatt ataattcaac gaaaatgaaa gaaaaaaaat ttacaacatg gacgcgaaat
     5641 ttaggggaat ttcgattaat aaaagattct atttcgccta aaatcacttc tgttaacttt
     5701 caggaaggta aatggttaag taaacaaaat gatttgcaat ttgaaattac agatgacctt
     5761 tcgggtatta aaacttatac gggttacttg aacggaaaat ggatattatt tgattatgat
     5821 tataagacca ataaaatcac acacgatttt tcagataatg tcgttataga aggacgaaat
     5881 gaattaaaaa ttgtagtaac tgataatgtc ggaaattcta ctatatttga atcacatttt
     5941 tttagaagtc agcaacctta aacctatctt ttgaaaacaa aacaaatttt attggctttc
     6001 ctgctttttt gtgtcggatt tgcctcaaat gctcaaaccg ccagaattaa aggaatcatt
     6061 ttagataaaa acaacaatcc ggttgaagga gtcagtatta attttcaaga taaaggcacc
     6121 gttaccaacg aaaatgggtt ttatatctta actattccgg cgaatcaaag aatcactttg
     6181 gtttttacac atatttcctt aaaaagagct actttacgaa tagaattaaa aactaacgaa
     6241 gattatgaat ataattttca aatgagtgat tcggatgagc ttttgggaga ggttatcatt
     6301 acttccggaa agcagaaacg aatagaaggg attacggcaa tcgaacccgc tattattcgc
     6361 caaattccag gtgcaaatgc cggtgttgaa aatattttaa agactttgcc cggtgtaaat
     6421 tcaaataatg aattaagtac gcaatattct gttcggggag gaaattatga tgaaaactta
     6481 gtttatgtta acgaaataga agtctatcgt ccgtttttga ttcgttccgg tcaacaggaa
     6541 ggattgagtt ttaccaatac ggatatggtg cagaatgttg atttttctgc cggcggtttt
     6601 caagctaaat atggggataa attatcttca gtattagata ttacttatcg aattccaact
     6661 cattttgcag cggctttaga agccagttta ttaggtggaa gtttaaccgt agaaggagtg
     6721 agcaagaatc aaaagtgggc taatatcacc ggggttcgct acagagataa tagtttattg
     6781 gttaacagtc aggaaacgga atcaaattat aaaccaactt ttgcagatat tcaaacgtat
     6841 ttgacttata cgcccaatgc aagatggcaa tggggatttt taggaaatat ttctcagaac
     6901 acctataatt atgagccttt gacacgtcag actaattttg gaacaatcga tgaaccgatt
     6961 gctttacagg ttttttatga aggtcaggaa aaagataaat accaaacttt atttggggcg
     7021 ataaaaacat cctttactgt aaatgacaat acaaccttaa agtttatcgg gtcagtttat
     7081 aacacacaag agcaagagta ttttgatata ttggctgctt attatttagg cgaagtagac
     7141 agtaatattg gttcagaaac gtttggagat gtaaccttta atcgtggtat tggtactcaa
     7201 cttaatcatg ggcgtaacaa tttagatgcc ttgattatta atgctgaagt gaaaggtttt
     7261 cacaaaatag aaacgcacgc cattgaatgg ggcgttaaat atacccgtga agacatcagg
     7321 gatagattag tggaatggga agtaatcgat tctgccggat tttcaattaa tccgcctcgc
     7381 gattttccgg taaatgatca gccttacaac ccttatacag gtcccttagt tccctatcaa
     7441 gatgttagag caactaattt tgtaactatc gatcgctttt caggatatgg gcaatggagt
     7501 aaaaaagcta gtttaggaac cagtgaagtt tggtataatg ccggcgtacg gatgcaacat
     7561 tggcaagttt caggtgacgg cgttaaatct caaacacaaa cagtatttag tccgagggca
     7621 caatttgcta ttaaacccga ttggaacagt gacatggttt ttagagtttc cggaggattt
     7681 tattaccaac ctccatttta tcgcgaactg cgggattatg aaggagtgat ccaaccggat
     7741 gtaaaagctc aacaatccat tcattttatt atgggtaatg actacagttt caaaatgtgg
     7801 aacagaccat ttaaattggt cagcgaatta tattataaaa cgctcgctga tgtaaatgct
     7861 tataccgttg ataatgttcg aattcgttac cgagctgata atgaaacgga agcctatgtt
     7921 tatggtgcag attttagaat taacggtgaa tttgttcccg gaacggaatc atggtttagt
     7981 ttaggctatt taaaaacaga ggaaaacatt caggataaag gatacatcgc caggccatcg
     8041 gatcaacggt tgaaatttgg cgtacttttt caggattatg ttccctctat tccaagttta
     8101 aaaatgtact tgaatttggt ttacaatacc gggcttccgg gtggttctcc atcctatgct
     8161 gatccatatg attaccaatt gcgtttgaaa gattatcgca gagctgacgt aggtttctcc
     8221 tatgttttaa cggaaaaaaa tgaaatcaga cctgaaggac attggttaaa acgttttaaa
     8281 gacttgtctg ttggattgga aatatttaat ttgtttaata atcaaaatgc cataacaaat
     8341 acgtgggtac gcgatgtgta taccaaaacc caatatggta ttccgaatta tatgacgacc
     8401 agagttttta atgtgaaatt gactgcacga ctctaactaa agattaaatt ttatatcttt
     8461 ggataataac tcacaatcgt atgaagactt ttattaatgc tgttttatta ttgattttag
     8521 tagtaacttt tggttgtaaa gaagatgaaa aggagaagcc aaaagtaacc tataaagaat
     8581 cgcctgcaca agtggaagta aaaaaagata ccactcaaat caaaattgct gatttaccca
     8641 tacacatgga aggaaccagt tttttgttgc atcctattgg agatgttcgc gtttatgatg
     8701 gtagttctaa atccagttac ggttcctcca gtacaagtcg ggttagttat tctatttcaa
     8761 attataatcg attcgaaatt acaggatact tgaataatat ccgttttcaa catctagatt
     8821 caacagcaac aagaccatta accgataaaa ttgttattat tcaaaccgct aattttttaa
     8881 atacaattgc ggaaaaaaca aaaaaacaga ttttagttta ttccatttct gatatggata
     8941 ctaacaaaga tggtaagcta gatgctaatg atatcaaatc gctttacatc agtgaaattt
     9001 caggaaatca ttttatgaaa ttatcacccg attttcaaga attaattgat tggaatttaa
     9061 ttgaagcaaa aaacagactt tattttagaa caactgaaga taccaacaaa aacggtgatt
     9121 ttgataaaga tgataccgta ttttattatt ttgttgattt gatggataaa gaatggaagt
     9181 taaacgaata taaaccgttt cattaaatca gaatatcact ttctaaatca gatttttcga
     9241 ttgaaaaatt aaaacccagt tgttccatta actgaatcac taaattttta taccagtttt
     9301 ctgatttcgg atgtatataa atcttttcaa tgagttggtg aatatcgaca tcaattttta
     9361 atccttcatt tatcacaata ttattttgag tcacatcaga aataatgcga acttcgcgtt
     9421 cgtattgaaa actttttcgt ttaaacaaaa agggaaaaaa agtgtcatca aacggaatat
     9481 attctttttt ataatcgata taatttactt ccccaatata ttgagttgtt ttagtttccg
     9541 gtttcatggc ttcctgtaat cttttaacgg tagattgtat agctaatcct tcgttgtttt
     9601 tggtgaaaat ttgccacatg gcaaacgatt catattcatt gatgtgccaa ctactaatca
     9661 caattttttc gcgatgggat ttataataat ctaaaaactc aggattgttt tcagcaattt
     9721 ttctgatttc ttcgtaggtt ggctcgctaa aggttccttc gtattggtct tcaaatttat
     9781 ccgacctaga cataaagagc ttttttgaaa gtaataaatc caagaattta gataagtcca
     9841 aatatttcca tacaattgtg tttggatttt ccggaagaat tatgtcagta ttatgaatat
     9901 acattttatt cgtaataagt taaaagttat agtctacttg aacctattct ttttgatttt
     9961 tattcgaaac tttgcatcat tactaatttg gaatacattc cattttgaga taacaattca
    10021 tcatgtgttc cttgttcaac aatttcgcct ttttgcatca ctactatcaa atctgctttt
    10081 tgtatagttg aaagacggtg tgcaataacg atagaggttc tgttttgcat catattttcc
    10141 agtgcaacct gaacaaactt ttcactttcg gtatcaaggg cagaagttgc ttcatccaaa
    10201 atcataattg gtggattttt taaaacagct cttgctatgg acaagcgttg tttttgtccc
    10261 cctgataatt ttccccctga atcgccaata tttgtttcaa tcccgagtgg taaatcattt
    10321 acaaattcaa aggcatttgc aattttcaat gcttcaatta tttcagtatc gctagcattt
    10381 tgctttccta ttaatatgtt atttttgatg gtatcattaa acaagatact gtcttgggta
    10441 accaacccca ttaaattccg aagagattct aaagaaatat ctttaatatc aattccgtct
    10501 attttcacgc atccatcttg aacgtcataa aaacgagtca ataaattggc gatggtgctt
    10561 ttgccacttc ctgactgacc tactaaagca accgtttttc ctttgggaat ttttaaagag
    10621 aaatttttca acacattttc ctcttcatat ttaaaattga tatcttcaat gctaatactt
    10681 tcatcaaaag aagttttttg taaagcattg ggtttgtcaa caattgaatt ttcaacttcc
    10741 aatacatcaa atacccgttc tgctgcggct aagcctgttt tcacttgata tgatgctttg
    10801 gaaatagctt ttgcaggagt taaaatatta taggctaagc ccatgtatgc taaaaaatga
    10861 gccccttcta aaattggact accatcagct aatttaccta ccaaaaccat atttccacca
    10921 taccataata ggatggctat gactacgatt cccatgaatt cgctcaatgg agaagataaa
    10981 ttattttttc tgcctatact attcgaaagt ttaaataaac gttgaatgga atttttgaat
    11041 ttacttctaa aattttcttc tgaattatag cttttaacaa cttttaaacc agccaatgtt
    11101 tcttccacaa tagaaattaa ataaccgttt tcatcttgtg ctcgttttga ttttgatttc
    11161 aaacttttac caattttagt aatgataaaa cctgaaaccg gaataaaaat aaaaacaaaa
    11221 agagtgagtt taaaactgat agctaccatg gcaccaatag taaaaaggat agtcataggt
    11281 tctttaacca ctaattccaa gatggaaaag aaagaatttt taacctcgtt tacatcgttt
    11341 agcattctgg ccatcacatc acctttcctt ttttcagaat aataggaaat gggtaaatcg
    11401 atgattttat caaacatttt attccttaaa tctcttagaa cgccattttt gataagcatc
    11461 aaatggttta aagccaaata gttaaagaaa ttttttagta aaaaagtgga aattactaaa
    11521 ctgactacca ataacaaggc atattgcgga ccgttatcat ccgataattt tgtgatataa
    11581 taatataaat aatctttccc gtaactagtt atttcccaaa tcgattgata ggttggtttt
    11641 gtagccactt tttctgcatt ttcgaacaaa acctgaagca tgggtatcaa agtaatgaaa
    11701 gaaagtgtgc taaaaagagc atacaaaata ttgaaaataa tatttaaaac cacatttgat
    11761 tgatagggtt ttagaaacgg aattatcttt tttaaatttt catccattag ttcaaatgca
    11821 tttctgctat gatagttgca attttttggt ctaaaattgc ttctacattt ttaaactgtt
    11881 caattgaatc taagctatta ttcacgctga aataaaattt gatttttggt tcggtaccac
    11941 taggtctagc acaaattttt gttccgtctt ccaaataata aatcagcaca ttggattttg
    12001 gaatttctaa caattctatt tcctctgtca ttaaattacg agcagtggaa ttgtcataat
    12061 cttcaataca aactacacgt tgcccattaa tttcttttaa tggattttca cgtaaatcaa
    12121 ccatcatttg tttaatttca tttgctccct caatcccttt tttggtaatg gaaatcaagt
    12181 attctttata aaatccaaaa tcaacatata aattaagtaa ttctttgtac aaagtgcttc
    12241 cggctgattt tgcttgtgca gcaatttcac aaacgagtag agtagcggcc acagcatctt
    12301 tatctctaac agcatcgccc accatgtagc caaaactttc ttcgccaccg ccaacaaatt
    12361 tttgattagg aaaatctttg atgaatttag caatccattt aaagcctgtt aaacccactt
    12421 tgcattctac accataagct gaggctaatt ctaacatcat tggtgtagaa actatggtag
    12481 agcctataaa ttcatttcct ttgaagcgtt cttctctttt ccattgttct aacaaaaagg
    12541 ccgtcatgat gaccatggtt tgatttccgt ttagcaattt cattttaccc tctgtatcac
    12601 gaacggcaac acctaaacgg tcggaatccg ggtcagtgcc aataacaatg tcggcattaa
    12661 ttttatcagc taatgctaat gccatggtta gtgcttccgg ttcttctggg tttggtgact
    12721 tcacagttgg gaaatttccg tccggttggg cttgttcttt tacaatgttg acatctgtat
    12781 agccggcttt agctaaaaca gcaggaattg ctttgatgga tgtgccgtga agcgaagtat
    12841 agacgatttt taaattttct tttgctgttt ttggagtgtt aaaactcgca ttttcgatag
    12901 ttgaagtaaa gaatgcttca tcaatattaa tgtcgatgta ttcaattaaa ctttcatttg
    12961 cagtaaattt aatttcatca taggctaatg cttcaattac ctcaattatt tcttcatctt
    13021 gtggtggtac taattgcccg ccatcttgcc aatacacttt atatccgtta tattcaggtg
    13081 gattgtgtga agctgttaaa actattccgg catgacaatt taagtgtttt aaagcaaagg
    13141 aaagttccgg agttggccgc atatcagaaa aaaggaacac tttaatcccg tttgcagaaa
    13201 atacatcggc aactactttc gctaaggtat cactattgtg acgacagtcg taagcaatca
    13261 caacttttaa cgcttctttt ggaaaggaat gttttagata attagaaata ccctgtgtgt
    13321 tttttcctaa tgtatatttg tttatacgat tggttccaac gcccatgatt cctctcattc
    13381 cgccggttcc gaattctaga tttttataga aactttcttc taattctttt ggagaagagg
    13441 tcatcatttt ttcgatttct tctcgtgttt gtaggtcaaa tgttggggtt aaccaaacgt
    13501 ttactttgtc gaggatgttt tgtggaatat gcataatctt attttttaaa tcctatttta
    13561 tttctttcga tttttggagt agctgtcttt tctttatcca ttaaataatt taaaatatta
    13621 tagatgtcag gaaattgttt ttctatttcg gtgattttat cattgagctc tttgtaattt
    13681 agtgcaaact gtcttaacga aacaaatgtt ctcataatgg caatgttgat agcgattgct
    13741 ttttccgaat ttaacacact tgaaagcatg gctatacctt gttcagtaaa tgcaaagggc
    13801 atgtatcgta atcctcctct ttttgaggtc acaaattgtg acctcaaact ttcaaactca
    13861 ttttgtgtaa gttcaaacat aaaatcaggg ggaaagcgat ttatatttct tctgacagct
    13921 tgttttaaaa cttttgtttc gatttcatat aattcagcta aatgaaaatc gagcattact
    13981 ttttgacctc ttaattcgat aatttgttgt tgaataattt ttaatttcat gattttggcg
    14041 tttataaatt ttaaatggct tttcgttttt tagttcatat ttaattcgtt tgaaatttta
    14101 taattttcat ttgattcttt atttcttaag attatttctc ctaaaaaccc ggctagaaat
    14161 aattgagaac caataatcat tgtcgttaag gcaatgtaaa agtaaggact attggtgact
    14221 aaatcatagg gtaaatcatt atatagccta tatagttttg agattccgat atatcctgtt
    14281 gcggtaaaac caatcaaaaa catcataact ccccaagctc caaaaagatg cataggtcgt
    14341 ttgccaaatc tggataaaaa ccaaatcgta attaagtcta aaaaaccatt gataaaacgt
    14401 tccatgccga atttggtttc accatatttt ctggcttggt gttttactac tttttcacca
    14461 atttttgaaa aaccggcatt ttttgctaaa acgggaatat agcggtgcat ttcaccggag
    14521 acttctatgt ttttgatgac cttatttttg taagctttta atccgcaatt aaaatcattg
    14581 agttgaactc cagacgtttt tcgggccgcc caattgaata attttgaagg taaattttta
    14641 gacatgactg aatcatatcg cttttttttc cagccggaaa ctaaatcaaa attttgggaa
    14701 gtaatgagtt catataattc gggaatctct tccggattat cttgcaagtc agcatccatc
    14761 gttatcacta catccccttg ggctttgtta aatccagcat gaagagcttg tgatttcccg
    14821 aagtttttaa gaaattttat tgcctttaca tttggatttt cattagataa ttgttcgata
    14881 actttccatg aattatccgt gcttccatca tcaataaaaa taatttcata ggtaaaatta
    14941 cttttattca ttacagtaac aacccaatca tgaagctcac gaagtgaatc ttcttcgttt
    15001 aaaagaggta taactatgga taaattcatt attctttagc agaagttggc gttttaaaaa
    15061 tcagagctac cagcaatcct aaaacagcat aaaaagcgat ttggaaaaag tatgattgga
    15121 attgtgataa aggaccaaaa ttatctttac tttcaatatc ggcaattgcc ttattaattt
    15181 cagcagccgg tacattaaat ttacccatca tttcttttgt catggtaatg acattttcgg
    15241 tcagaacatt tttagcttca ggatcaatta cattaaacag aatgatgttg tataaaacat
    15301 aaatggcaat accaacaatt atgggtaaga taaagcttaa aaaagcttct ttatagctca
    15361 tttgaccttt taatgctttt ttgtttgtta tagagcaata aatagctata ccaattatga
    15421 tgacgaaatt tactattccc acccaccaat ttgtgaataa cgataaatca acggtataca
    15481 tggttgtggt aattaatact aaaattacac ccattataat gcctaaagta aagccgttct
    15541 ttttgatact attttccata ttgtttattt taaagtacaa atataacaat attccaatta
    15601 tggttcagaa aatcaagatt taaaagactt caaaaaaaag taattaaaca tttgattatt
    15661 aaaaaatagt tgtaaatttg cacacttaaa aaaagtaaaa gattataaac aaaaagttgt
    15721 gtgtttttta caccttttcg ttgaagaaaa gcttcggaac ggcatacaac cattaataga
    15781 aaaagattta cacaaaatga aaaaaggaat tcacccagaa aattacagat tagttgcctt
    15841 caaagacatg tcgaatgagg atgtatttat tactaagtca actgtagaaa cgaaagaaac
    15901 aattacacac gaaggagttg aatatcctgt gtttaaaatg gagatttcta gaacatctca
    15961 cccattttat accggtaaat ctaaacttat tgatactgca ggtcgtattg ataaattcaa
    16021 aaacaaatat gccaacagag caaaataatt tttgcctcaa attttataaa aaaaatcccg
    16081 agcaatcggg attttttgtt tttctaaaag tttaaatttt taattgtttt tgaataaatc
    16141 ttctaagggt ttcaattgtt ccatcccaat atccgatttt tgaagagcag aaaacaaagt
    16201 cataaaatct tccggattca ttttatctcc taatatacga acaactgcaa atccgttttc
    16261 atttttatta ccatataaaa cgaattcttc gatagcatca tcttttccaa caaaataaac
    16321 agcagcacct tcttttccgg agccaaattt cattaattgt tggtactttt catcttttaa
    16381 aatttcggtt actttagttc gttccgtatc aaattttact ttgttttgtt catttgcctt
    16441 aaatgctaaa acattcattt ttttaaacgt ttttaaggct tcttgttgtt cgccggtcaa
    16501 agcatccggt ttgatattta aaatactggg agaaacatcc acggaaataa aatcactgga
    16561 ttcagtgttc tctacgaaat acttttgtaa agttggattc tgttcacaag ctatcaaact
    16621 gactagcata agtgaggcta ttattttttt catttaatta ttttttaatg tttttaaaat
    16681 tcgttcttca ttttcagaaa gaggcatcgc attatttgtt tcccaaaaat tttctttgta
    16741 attcattccg tttgggtata aacttctttc ttgataagtg gtgtttttat ccggtgaagt
    16801 gataatagta tcctcaaaat ttgttacaac taaatcgctt atcgacatca gtcggtcatt
    16861 catttgacca ccaaatttga tgtaaacatc caatagtgtt tttttataac ttggaatata
    16921 ttttccgttt acatatttat atgattgttt aatctggatg tcataaaaag catatttaaa
    16981 caacaacata ttggttattt caacattttt tttatgtttt tccgacattt ttatatctat
    17041 atccagcaca atacggttaa cagggtcgta cgtgattttg ccttccaata aaacttcatc
    17101 tacgtttggt ttaggaatta tataaattac ttcaatagca tttccgtatt ggtctttttt
    17161 tgaacggagt tcaaagtcat aatttggttc gtttttttta ctcaagatac ttttttcaat
    17221 ataatcataa ctaaaaaatc catttgctgc tttttgaata tcaaaaaagg aatccatttc
    17281 tgccaaatct gcttttcctt ttttctcgat ttcatcagca ccaattagtt gataagccct
    17341 actttgattc acaataagtt ttgcttccag tttatctttc tttttaggtt gtaaataaaa
    17401 atcaattaat ccgtcggcaa attttgaata tttattgttg atttttacaa attcacgata
    17461 ataggtttcc aattttacac ttttgtctaa taatgtttta gaatgactta tgatgccgga
    17521 taaaattgta gcaaccggtt tgttggtcac aattacttct tccaaagcaa tttcatctat
    17581 ttctaaaaat acggttgtaa aagtggaatc aatttttgcc acttcaaatt tcttagattg
    17641 atagcctaac gaactaattt caatggtcgt tacatttttt gaaatgtaaa atttaaagac
    17701 accatcttca tttgaaataa ctccttgagc tgtttgtaag gcaataacat ttgcaaacgg
    17761 aatcgattct ttggttgatt tatccactat tttgccttca aaaacagttg attgagcatt
    17821 tactgtaaaa agagacaaac taaaaaagaa caatactatt tttctcattt aaatgatggg
    17881 taaggaggga acataacagt tgaagacatt ccagaattat ataaaaaaaa gactttgtca
    17941 aatcaatttt tgacaaagtc ttgaattaat tatttttttc ctttggttga aacttttttc
    18001 aaatcatcac caccgggaag attcattttt tcagttaaag ccgatatttc atttaaatca
    18061 aaatttccgg ttaacgataa taaaacggtt tctttatctt tcgggccacc ttccataaac
    18121 ataaataatt cgcgtacttc ggtgtctttt gctccggatt tcatgtaaat tctaacgttt
    18181 tttccgccat cagaaacacg cattaattct tctaatccgg ctgtttttaa atatttatca
    18241 gcagtcgttc gcatgtcagc tgtgattttt gaattagaag tgacaaatac ttttaaattg
    18301 tctaattttt ttatcaggtt caaatattgt tgggcttctt tgtcagcagg atccattttt
    18361 actttactca tcatttcaaa catttttttg tttacgatga tggtgctaac ttcttcctga
    18421 ccgtcaaatt tgtcaaaaac agattgacca aacatgatgg ttgaaactaa aaatacgact
    18481 aaggttgtta caaattttat catttttttt attttttaaa tattaattgt tttgattttt
    18541 catactcatt taaataggca atactttcaa cgccctcgtt cacattttct gaaagcaaag
    18601 ccaaagcctt ttgtgtctct tcgaaggctt cttccggact gtcaaaactt cctaaatcgg
    18661 aaactacctg agcaggtgcg ggagagggtt tgtaattcag ataagcaaaa agcccgccaa
    18721 agaaaactaa acttgccgca atcgctatcc aagctaccga aaaccgtttt tttgaaggta
    18781 acgaaagttc tttgttgaat agttccttat cattgacggt aagataatta aacaacggtt
    18841 tgtaatgctc taaatgagcg gctactgttt cagttgaaaa gtactgtttc aactgttttt
    18901 cttccgcaag tgttgtatct ccttgaaagt atttttctat taattcttct attctagcta
    18961 attccatatt gatgggtttt agtcattttt tctctaattg tttttcgggc tcttgataaa
    19021 gcaactcgaa tagctgtttc attcatgtcc agcatttttg ctatttcttc aaaatcatat
    19081 tgttcgatat cccgcatttg tatgattaac ttttgttgtt ccggaagctc attaattatt
    19141 ttttcaaccc aactccaact gtcttggtct tccatttttt gatctaaacc ggcttctctg
    19201 tctgtaaaat tggtgtgaac aattttaagg tttgaagctc ttttcgactt caattggtct
    19261 aaacaataat tttttgtcat tgtcattgct aaagcttcaa catttttata ttgtcctaat
    19321 ccatttttct tatcccataa ctttaccaaa atatcctgag tggcatcctg agcttcctca
    19381 ctacttacca gcaatcgctt ggctaaccga aagactttgt ctttgaatgg ttcaatgagg
    19441 tgtataaact cattttgatt catctatcag gttttaatgg ttggttatat aagcaagacg
    19501 aatgaaggtc ttatttgtta caaataaatt aatt
//
LOCUS       DAOTCW010000036        19330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1543_length_19330_cov_36.370947, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000036 DAOTCW010000000
VERSION     DAOTCW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 19330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19330
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1543_length_19330_cov_36.370947"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..608
                     /locus_tag="P5335_04640"
     CDS             249..608
                     /locus_tag="P5335_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74199.1"
                     /translation="MKRIVLVLFAVLFLVGCESESVETQPQALTIPEWLKGNYEGVHT
                     KENLVISNQKITFKVEQTVYEFEVNQILSHQEEENRFIIQTASDILIFNKTTLQTEIN
                     FQFNELYLGWFRKRLVQ"
     gene            complement(690..1880)
                     /locus_tag="P5335_04645"
     CDS             complement(690..1880)
                     /locus_tag="P5335_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRZ74200.1"
                     /translation="MPKISKKGLLMPESPIRKLVPYAEIAKKRGTKVYHLNIGQPDIK
                     TPEVAMNSIKNLDIKVLEYSHSAGFESYRRKLSDYYQKHSLPINFEDIIITTGGSEAL
                     LFAMGSTMDTDDEIIIPEPFYANYNGFSTASGVKVIPVISTIENGFALPPIAEFEKLI
                     TSKTKAILICNPGNPTGYLYSKEEIFQLAELVKKHDLFLIADEVYREFIYDGEEHFSV
                     MNVPGLEQNAIMIDSVSKRYSMCGARIGCIVSKNKELMTTAMKFAQARLSPPTFAQIA
                     SEAALDTPQSYFDEVITEYKDRRDTLIEELNKIDGVKVGSPKGAFYCIAKLPVKNADH
                     FAQWLLETFDFNGETVMVAPAAGFYSTPGVGLDEVRIAYVLKKDDLVKAVQILKEALK
                     VYKN"
     gene            2002..3312
                     /locus_tag="P5335_04650"
     CDS             2002..3312
                     /locus_tag="P5335_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRZ74201.1"
                     /translation="MKIQKIIFLLLFFSIGVFGQSSFQFISDKDKIKIPFQFINNLIF
                     VPIQVNGVELTFLLDTGVEETILFSLLDKDQVEFNNVEKIKLKGLGNAEFIEGLKSSK
                     NKIVVTPTFFDPSHTIYIILNEDINISSGVGIPVNGIMGYGFFKDYPMEIDYVNHYIT
                     VYKHKSKAVIKKYKKFKKYPIILENNKPYIETKFAIDTTFIEAKLLIDLGNSDAVWLF
                     QNKLKSFELPEPNFSDFLGQGFSGPIFGKRAKLAKFSLGEFVFENPIVAFPDSLSTQS
                     VKLVKNRVGSIGAEILKRFTLFIDYRNSEIAIRKNRNFSVPFNYNMSGLEIHHIGLKW
                     IPEKIQFNKSQLIQSTESVGFYYKFVLKPIYVISNVREDSPAAECGLKKEDIIVSINK
                     KEAHLLSLQEINNLLKSEEGKLIKMEVQRGSQFLNFSFVLKSIL"
     gene            complement(3344..3778)
                     /locus_tag="P5335_04655"
     CDS             complement(3344..3778)
                     /locus_tag="P5335_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRZ74202.1"
                     /translation="MKKLFFFTVVSLFSFSVFAQNGPKIVFKADDNTIDYGKVYKDSD
                     NGVRVFEFTNTGNAPLIITNVQSTCGCTVPSKPKAPIMPGKSDKIEVKYNMATGPIRK
                     SITVESNAVNYENGTVHLKIKGEVLERPEVNLLEKKNVGPMQ"
     gene            3962..6595
                     /locus_tag="P5335_04660"
     CDS             3962..6595
                     /locus_tag="P5335_04660"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006264423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRZ74203.1"
                     /translation="MSIPTQFDAKQSEQKWYNYWMKNNYFHSEPDHRTPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDVLIRRARLKGMNACWVPGTDHASIATEAKVVAKLKAEGINK
                     EDLTREEFLKHAWEWTDKHGGIILEQLKKLGCSCDWDRTKFTMDDDMSASVIKSFVDL
                     YNKGLIYRGYRMVNWDPEAKTTLSDEEVIYEERQGKLYFLNYKIEGSTESVTIATTRP
                     ETILGDTAICINPNDERFAHLKGKKAIVPICNRVIPIIFDDYVDMEFGTGCLKVTPAH
                     DVNDKELGNRHNLDIIDIFNENATLNSYGLHYQGKDRFVVRDEIVTELESLGHIAKIE
                     NHINKVGTSERTKAVIEPRLSDQWFLKMEDLVKPAFKAVMETEEIKLYPNRFNNTYKH
                     WLENIRDWNISRQLWWGQQIPAYFYGDGKEEYVVAETIEEALELAKAKTNNPQLTIHN
                     LKQDADALDTWFSSWLWPMAVFGGVLEPENKDFKYYYPTNDLVTGPDILFFWVARMII
                     AGYEYTGKKPFTNVYLTGIVRDKQRRKMSKSLGNSPDPLDLIEKYGADGVRVGLLLSA
                     STGNDIMFDEELCNNGKAFANKIWNAFKLINGWEVSDSISQPESSKIAIAWYEAKLEK
                     TLAEIEDQFEKYRLSDALMAIYKLVWDDFCSWFLEMIKPSYQQPIDKVTYTRAIAALE
                     ANLKLLHPFMPFLTEEIWHHLAERSPEQALIISEWPTIANTNVNLIADFEFASEVIAG
                     LRTIRKDKNIPFKEAIELKVINHENASKEFDSVIVKLGNVSSIDYISEQENGALTYRV
                     KSNEYFIPISGAINVEEEVAKLQEELKYTNGFLNSVRAKLSNEKFVSGAPEKVIEMER
                     KKEADALAKIATIEQSLASLK"
     gene            complement(6668..7444)
                     /locus_tag="P5335_04665"
     CDS             complement(6668..7444)
                     /locus_tag="P5335_04665"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009744838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRZ74204.1"
                     /translation="MKKNVILSALVITTLISCNNNKKLEEVVEVPLPEKEVVVTEMGN
                     PADVKVDEGGAFEMVALPYAYNALEPYIDAKTMEIHYSKHHLAYANNLNKAIAGTEMA
                     NMSIEDIFKKLDPENKAVRNNGGGYYNHNFFWEIMGANKGGEPKGTLAEAITKDFGSF
                     ADFKTQFIEAGMKQFGSGWAWLILDKTGKLTIISTANQDNPLMTKLGNTGTPILNVDV
                     WEHAYYLKHQNKRKDYLEGFFNVINWDKVAEKYTTATAVK"
     gene            complement(7449..7718)
                     /locus_tag="P5335_04670"
     CDS             complement(7449..7718)
                     /locus_tag="P5335_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HRZ74205.1"
                     /translation="MVEKDLDTRFREAFEKASNMTETLPPDVMLRIYAYYKQATHGMV
                     GYTQPSSNDLRNAFKTNAWMQISHISSDEAKELYIETINSLLNKK"
     gene            complement(7722..8372)
                     /locus_tag="P5335_04675"
     CDS             complement(7722..8372)
                     /locus_tag="P5335_04675"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009419689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRZ74206.1"
                     /translation="MFHKEGAKIILIATVIVTAWILLADAFISLVWLKMTVQTIGLLF
                     LILILQFFRNPKRNTPIDDNHIIAPVDGKVVVIEEVFEPEYFKDKRLQVSIFMSPINV
                     HVTRYASNGKINFSKYHPGKYLVAWHPKASTENERTTVVIENRIFGEILYRQIAGALA
                     RRIVNYAQEGMQVVQGTDAGFIKFGSRVDLFLPLGTKIKVTLNQTVKGGETVVAEK"
     gene            complement(8362..9168)
                     /locus_tag="P5335_04680"
     CDS             complement(8362..9168)
                     /locus_tag="P5335_04680"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRZ74207.1"
                     /translation="MNETVTRALSGSVYIVLLVGCILHSELSFYILFGGFLGLTTFEF
                     CKLVDLPKSIPAAFAVALFALFCDSTFYPFQDGIITGFTVLVSFKLLYSLFSTKEKTI
                     TNFEKWLYLIGYIIFPFVIITKIPFGLSGYNPKIIIGIFVLIWTNDTFAYVVGKSIGK
                     HKLYERISPKKTIEGFIGGFVFTIIAGIIIAKYLLNTSNLTIWIGTAVLVSIFATLGD
                     LIQSRFKRIAGVKDSGKIMPGHGGILDRLDSMIFVAPFIFLFYQILNYVS"
     gene            complement(9170..9772)
                     /locus_tag="P5335_04685"
     CDS             complement(9170..9772)
                     /locus_tag="P5335_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HRZ74208.1"
                     /translation="MSLFKKLFGSGNEASEENKEKDASPFNPDNKIPVDELFTFNFKK
                     NGGKFLYCDNLEEIKEQFLNILEENDWFECEAVCFEPKLFHLLDENKLNFKNPVNPKF
                     LLTSCENLIADEGSVLLTSNQIKQNKPHELPANMIVFATTSQILESKSDGLREIKKRY
                     VKDYPTNITTIKYFEKAKEEDFLQYGSAAKNLYLLLLEDL"
     gene            complement(9891..11843)
                     /gene="ftsH"
                     /locus_tag="P5335_04690"
     CDS             complement(9891..11843)
                     /gene="ftsH"
                     /locus_tag="P5335_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRZ74209.1"
                     /translation="MAKENNSTPNSNKFKFNPWLLYFIVAGIFIFISIISGGSSFQEP
                     GKLTISKFNEYLENGEIEKVLVYNKGENISEAEIYIKKDALKDQKHKGVVKDVLGREN
                     TGAHYLLPNIADGKLFQEKLELAKKENKLADYNYLVKSNWSGILINLLPIILIVVIWI
                     IIMRRMSGGSGGGGGGQIFNIGKSRAKLFDEKTDVKVTFKDVAGLEGAKEEVQEIVDF
                     LKNPEKYTTLGGKIPKGALLVGPPGTGKTLLAKAVAGEAKVPFFSLSGSDFVEMFVGV
                     GASRVRDLFKQAKDKSPAIIFIDEIDAVGRARGKNNMSGSNDERENTLNQLLTEMDGF
                     GTNTNVIVLAATNRADVLDKALMRAGRFDRQIYVDLPDIKERKEIFEVHLAPLKKQEG
                     LDTEFLAKQTPGFSGADIANVCNEAALIAARHDKKAVDKQDFLDAVDRIVGGLEKKNK
                     IVTPDEKRAIATHEAGHATVSWMLEHAAPLVKVTIVPRGQSLGAAWYLPEERLIVRPD
                     QMLDEMCATMGGRAAEKVIFNRISTGALSDLEKVTKQARAMVTIYGLNEKLGNVTYYD
                     SSGQSEYSFSKPYSEETAVTIDKEISNLIESQYQRAIKLLEDNKDKLIQLADILIEKE
                     VIFKDDLETIFGKRPFGDESHVSVGE"
     gene            complement(11870..12241)
                     /gene="rsfS"
                     /locus_tag="P5335_04695"
     CDS             complement(11870..12241)
                     /gene="rsfS"
                     /locus_tag="P5335_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRZ74210.1"
                     /translation="MANKKVSKDELLINIIKGIEEVKGNDIDILDLRDIENTVCDYFI
                     ICNGTSNTQVNAIVNSVQKTVSKELKDKPWHVEGADNAEWVLMDYVDIVVHVFQKHVR
                     EFYNIEGLWGDAKITTIPNKI"
     gene            12336..13064
                     /locus_tag="P5335_04700"
     CDS             12336..13064
                     /locus_tag="P5335_04700"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRZ74211.1"
                     /translation="MHIIKLSATPSTNNYLKQLYSQQVVANFTIVVASNQLKGKGQMG
                     TSWESESGKNLTFSILLKDLIVDVKQLFTLNILVSLALVEVLERYKIPNVTVKWPNDI
                     MSGNLKLGGILIENSIKSDGEVISILGIGLNINQLYFEKAPNATSLHLISGRNYNLDD
                     LLLDFCTSIKQNLTMMEKDSEKIWVNYHQKLFKKGIPMVFEDVHGIKFMGIIQKVSND
                     GLLHILLEDDSIAKFGVKEIKMLY"
     assembly_gap    13234..13333
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13334..>15007)
                     /locus_tag="P5335_04705"
     CDS             complement(<13334..>15007)
                     /locus_tag="P5335_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRZ74212.1"
                     /translation="TPEDFSEEQIMMRDSVKEFVDKEIWPNKNRFEKKDFAFTEEVMR
                     KAGEMGFLSVAVPEAYSGMGMGFVDTCLVCDYISGATGSFSTAFGAHTGIGTMPITLY
                     GTEEQKKKYVPKLASGEWFGAYCLTEPGAGSDANSGKTKAVLSADGTHYNITGQKMWI
                     SNAGFCSLFIVFARIEDDKNITGFIVENDPSNGISMNEEEHKLGIRASSTRQVFFSDT
                     KVPVENMLAGRGEGFKIAMNALNVGRIKLAAACLDAQRRVTSNAINYANERVQFNTPI
                     SSFGAIRYKLAEMATSAYAGESATYRAAKAIEDRINARVAEGTSHQEAELKGVEEFAI
                     ECSILKVAVSEDVQNCADEGIQIYGGMGFSEDTPMESAWRDARIARIYEGTNEINRML
                     SVGMLIKKAMKGHVDLLGPAMKVQEDLMGIPSFDTPDYSELFSEEKEMVGKLKKAFLM
                     VAGAAVQKFGMDLDAHQQLLMAAADMLIEIYMAESTVLRTEKLAKKVGEEKAKEQIAM
                     AKLYLYKAVDIVTQKGKESIISFAEGDEQRMMLMGLRRFTKYTNMPNIVG"
     assembly_gap    15008..15107
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15108..>16243)
                     /locus_tag="P5335_04710"
     CDS             complement(<15108..>16243)
                     /locus_tag="P5335_04710"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRZ74213.1"
                     /translation="MKTAYIVKAYRTAVGKAPKGVFRFKRPDELAAETIQFMMNELPD
                     FDKKRIDDVMVGNAMPEAEQGLNVGRLISLMGLKVTDVPGVTVNRYCASGLETIGMAT
                     AKIQSGMADCIIAGGAESMSFIPMGGYKPTPDYAVAASGNEDYYWGMGLTAEAVAKQF
                     NVSREDQDEFAFHSHQKALKAQAEGKFDKQIVPITVEQTFINENGKKETKSYIVKKDE
                     GPRADTNVAALGKLKPVFSADGSVTAGNSSQMSDGAAFVLIMSEELVNELGIKPIARL
                     VNFASAGVEPRIMGIGPVKAIPKALKQAGLTLNDIDLIELNEAFASQSLAVVRELGLN
                     PDIVNVNGGAIALGHPLGCTGAKLSVQLFDEMRLRGSKYGIVSM"
     assembly_gap    16244..16343
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16344..18721)
                     /locus_tag="P5335_04715"
     CDS             complement(<16344..18721)
                     /locus_tag="P5335_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HRZ74214.1"
                     /translation="MKRTIKKVAVIGSGIMGSGIACHFANIGVEVLLLDIIPNALNEP
                     EQQKGLTLDSKPVRNRIVNDSLTNALRSNPSPIYSQQFAKRITTGNTTDDMPKIATVD
                     WIIEVVVERLDIKKLVFEQVDKYRKPGTLVTSNTSGIPIQFMSDGRSEDFQQHFCGTH
                     FFNPARYLKLFEIIPGPKTSQSVLDFLTNYGSQFLGKTSVVAKDTPAFIGNRIGIFGI
                     MSLFHLVQEMGLTIEEVDKLTGPVIGRPKSATFRTVDVVGLDTLVHVANGIYQNCISD
                     EAHSLFKLPSFIDKMMENKWLGSKTGQGFYKKVDKDILSLDLTTLEYRPNKKASFATL
                     ELTKTIDKPIDRFRVLVKGKDKAGDFYRKNFAAMFAYCSNRIPEISDELYKIDDAMKA
                     GFGWENGPFEIWDSIGVAKGIELMKAEGYEPATWVTDMLAAGSNSFYTIKNGATHFYN
                     IPSKTQEKIPGQDAFIILNNIRESHKVWGNADATIQHLGDGILNLEFHSKMNTIGGGV
                     LQGINKAIDLAEKEYNGLVIGNQGTNFSVGANIGMIFMMAVEQEYDELNMAIKMFQDT
                     MMRCRYSSIPVIVAPHGMTLGGGCEMSMHADRVVAAAETYIGLVEFGVGVIPGGGGSK
                     EMTVRASDLFHKNDVELNVLQEYFLTIGMAKVATSAYEAFDYGILQKGKDIVVVNRDR
                     QIAVAKQVALQMAEQGYTQPIQRKDIKVLGKQALGMFLVGTDQMQAGKYISEHDRKIA
                     NKLAYVMAGGDLSEPTLVSEQYLLDIEREAFLSLCTERKTLERIQFMLTKGKP"
     gene            complement(18782..19213)
                     /locus_tag="P5335_04720"
     CDS             complement(18782..19213)
                     /locus_tag="P5335_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRZ74215.1"
                     /translation="MKDKTIDYILRATWQSVARMYNDEASKYEGSMAIGFALLSIDKE
                     DGTPSTYISNRMGMEATSLTRTLKTLEEKKLIIRKKNPDDGRGVLIYLTELGKEKREL
                     SKNTVIKFNETIKQNIPEDKLNTFMEVAEIINELITEKKIF"
BASE COUNT         5759 a         3294 c         3411 g         6566 t
ORIGIN      
        1 caacagatta ctggtttacg gtggagtatg aagaaaacgg tgaatctaaa atttttaaag
       61 ctcacttctc gttaaaacga taaaaaataa tattaaaaat ttaactcctg atgaacgtca
      121 ggagtttttt ttatcgttaa actacaccga cagttaattt aatcgttttt attgccgttt
      181 aaacgatttt aaatgataat catagtggta agcgttagat ttacagcagg ttattaaata
      241 ctgcctagat gaaaagaatt gttttagttt tatttgctgt gttattttta gtaggatgtg
      301 aatcggaatc ggtagaaact caaccacagg cattaactat tcctgaatgg ttaaaaggga
      361 attacgaagg agtacatacg aaagaaaatc ttgtgatttc gaatcaaaaa atcaccttta
      421 aggtggagca aacagtatat gaatttgaag taaatcaaat actttcacac caagaggaag
      481 aaaacagatt tataattcaa accgcgtcag atattttgat ttttaataaa accactttgc
      541 aaacagaaat aaattttcaa tttaatgaat tatatctagg gtggtttaga aaacgacttg
      601 tccaatagat tttatcttag ttaattataa aaagaaaaac ctttcaagtt tgtttacttg
      661 aaaggttttt aaatagcaca aaattataat tagtttttat aaacttttaa agcttcttta
      721 agtatttgaa cagctttaac taaatcgtct tttttcaata cataagcaat tctaacttca
      781 tctaaaccaa ctcctggagt agaatagaaa ccggctgcag gagcaaccat aaccgtttcg
      841 ccattaaaat caaaagtttc taaaagccat tgggcaaaat gatcagcatt tttaacgggc
      901 aactttgcaa tacaataaaa ggcaccttta ggggatccaa ctttaactcc atctattttg
      961 ttgagttctt ctattaaagt atctctgcgg tctttgtatt cagtaattac ttcatcaaaa
     1021 taactttgag gagtatcgag agctgcttca ctcgcaattt gagcaaaagt gggaggactt
     1081 aatcttgctt gagcaaattt catagctgtt gtcatcaact ctttattttt tgaaacaatg
     1141 cagccaattc tagccccaca catactgtaa cgtttagaaa ccgaatctat cataatggca
     1201 ttttgttcca atcccggtac attcatcaca gagaaatgtt cctcaccgtc ataaatgaat
     1261 tcgcgataaa cttcatctgc tattaagaat aaatcatgtt ttttaaccaa ttcagctaat
     1321 tgaaaaattt cttctttgga atataagtat ccggttggat ttccgggatt acaaatcaaa
     1381 atagctttag tttttgaggt aattaatttt tcaaattcag caataggagg aagagcaaat
     1441 ccattttcaa tggttgaaat aacaggtata acttttaccc cggaggcagt agaaaaaccg
     1501 ttgtaattgg cgtagaatgg ttccggaata ataatttcat catcagtatc cattgtgctt
     1561 cccattgcaa aaagtaatgc ttctgaccca cctgtagtaa taatgatatc ttcaaaatta
     1621 atgggtaaac tgtgtttttg gtaataatca gacaattttc tacggtaact ttcaaatccg
     1681 gcagaatggc tgtattccag tactttaata tctaaatttt ttatcgaatt cattgctact
     1741 tccggtgttt taatgtccgg ttgtccaatg tttaaatgat atacttttgt tcctcttttt
     1801 ttagcaatct cggcatacgg caccagtttt ctgataggtg attccggcat gagtagacct
     1861 tttttggaaa tttttggcat aattaaatta tttgaactgc aaatttgcaa ctttttttat
     1921 tcagacccaa tcttttaaag gatatattta aggataagaa aattcatttc tttttttgta
     1981 attttatgtt ttatatattt tatgaaaatt caaaaaatta tatttctact tcttttcttc
     2041 agtattggag tttttggtca atccagtttt caatttattt cagataaaga caagatcaaa
     2101 atcccttttc aatttatcaa taatttgatt tttgtgccta ttcaagtgaa cggggtagaa
     2161 ttgacttttt tattagatac aggcgtagaa gaaactattt tgtttagttt gttagataaa
     2221 gatcaggtgg aatttaataa cgtggaaaaa ataaaactca agggtttggg caatgctgaa
     2281 tttattgaag gattaaaatc ctctaaaaat aaaatagttg ttacgccaac tttttttgac
     2341 ccgagtcata ccatttatat tattttaaat gaagatatta atatttcttc cggagttgga
     2401 attcctgtta atggtatcat gggctatggt ttttttaagg attatccaat ggaaatcgat
     2461 tatgtgaatc attacattac ggtgtataag cataaatcaa aagcagtcat taaaaaatat
     2521 aaaaaattca aaaaatatcc aattatactt gaaaataata aaccttatat agaaacaaaa
     2581 ttcgctattg acacaacatt tattgaggcg aaattgctga ttgatttagg gaatagtgat
     2641 gccgtttggt tgtttcaaaa taagttaaag tcttttgaac tacccgaacc taatttcagt
     2701 gattttttag ggcaaggatt tagtgggcct atttttggga aacgtgcaaa attggcaaaa
     2761 ttttcattag gagaatttgt atttgaaaat ccaattgtag cttttccgga ttcactctct
     2821 actcaaagtg tgaaacttgt taaaaataga gttggctcta ttggtgctga gattttaaaa
     2881 aggtttacgt tatttattga ttatagaaat tcagaaatcg caattcggaa aaacagaaac
     2941 ttttctgtac cctttaatta taatatgagt ggtttagaaa ttcatcacat cggattaaaa
     3001 tggattccgg aaaaaataca attcaataaa tcgcaactga ttcagagcac agagtcagtt
     3061 ggtttttatt ataaatttgt gctgaaacct atttatgtta tttcaaatgt tcgtgaggat
     3121 tcgccggcgg cagaatgtgg attaaaaaag gaagatataa ttgtatctat taataagaaa
     3181 gaagctcatt tactctcact tcaggaaatt aataacctat taaaatctga agaaggcaag
     3241 cttataaaaa tggaagttca aagaggaagt caatttctta attttagttt tgttttaaaa
     3301 agcattttat aaaaaaaagc tcttcaaaag aagagcttaa tgtttattgc attggaccta
     3361 catttttctt ttccaataaa tttacttccg gacgttctaa aacttcacct ttaattttta
     3421 gatgaactgt tccgttttca taatttacag cattactttc aacggtaatg ctttttcgaa
     3481 tagggcctgt cgccatgtta tatttaactt caattttgtc cgacttcccc ggcatgattg
     3541 gtgctttggg ttttgatgga acagtacaac cacaagttga ctgtacatta gtgataatta
     3601 agggagcatt tccggtatta gtaaattcaa aaactctgac accattgtct gaatctttat
     3661 atactttacc ataatctatt gtattatcgt ctgctttaaa aacgatttta ggaccatttt
     3721 gagcaaatac agaaaaactg aataagctaa caacagtaaa aaagaataat tttttcatgg
     3781 ttatgtgttt ttgggttgtg taaaattaat cactttttat taaagtacaa atacaaattt
     3841 gatttttaat actctagcga aatcattact tttgcaagat agtacaaaaa tcagaccaaa
     3901 attgttaatt aaatataacc aattatcaat tatagttaac tagtaacata accaatttca
     3961 catgtcaatt ccaactcaat ttgatgccaa gcaatctgaa caaaaatggt ataactattg
     4021 gatgaaaaac aattactttc attcagaacc ggaccataga acgccttata ctatcgtaat
     4081 cccaccccca aacgtgacag gtgtattaca catgggacat atgttgaata ataccattca
     4141 agatgtattg attcgtcgtg cccgtttaaa aggaatgaat gcttgttggg ttcccggaac
     4201 tgaccatgcc tctattgcta ctgaagcaaa agtagtagct aaattgaaag cagaaggaat
     4261 caataaagag gatttaactc gagaagaatt tttgaaacat gcttgggaat ggacagataa
     4321 acacggtgga attattttag aacaactgaa aaaattgggt tgttcttgtg attgggatag
     4381 aacaaaattc acaatggatg atgacatgag tgcttcggtt atcaaatcgt tcgttgattt
     4441 atacaacaaa gggttgattt atcgtggcta tagaatggtc aattgggatc cggaagcaaa
     4501 aaccacactt tctgatgaag aagtaatcta tgaagaacgc caagggaaat tatattttct
     4561 gaattataaa attgaaggct ctacggaatc agtcactatt gcaacaacac gtccagaaac
     4621 aatattagga gacactgcaa tttgtattaa cccaaatgac gaacgttttg cccatttaaa
     4681 aggtaaaaaa gcgattgttc ctatttgtaa tcgagtgatt ccaattattt ttgatgacta
     4741 tgtagatatg gaatttggta ccggttgctt aaaagttaca ccggcacacg atgttaatga
     4801 taaagagtta ggaaatagac ataacctaga tatcattgac atttttaatg aaaatgcaac
     4861 gttaaacagt tatggattgc attatcaagg aaaagaccgt tttgtggttc gagatgagat
     4921 tgtaactgaa ttagaatctc ttgggcatat tgccaaaatt gaaaatcaca ttaataaagt
     4981 tggaacttcc gaacggacaa aagcggttat tgaacctcgt ttgtctgatc aatggttttt
     5041 gaaaatggaa gatttggtta aaccggcatt taaggccgta atggaaactg aagagataaa
     5101 attatatcca aatcgattta ataataccta caaacattgg ttagaaaata tccgagattg
     5161 gaatatttcc cgacagttgt ggtggggtca acaaattcca gcatatttct atggagacgg
     5221 aaaagaagaa tatgtggttg ctgaaactat cgaggaagcc ttggaattag ctaaagcaaa
     5281 aaccaataat ccacaactta caatacataa tcttaaacaa gatgcagatg ctttggatac
     5341 ttggttttct tcttggttat ggccaatggc tgtttttgga ggtgttttag aaccggaaaa
     5401 taaggatttt aaatattatt atccaacaaa tgatttagtg accggtcctg atattttgtt
     5461 tttctgggta gctagaatga ttattgccgg ctatgaatat acaggtaaaa aaccatttac
     5521 aaatgtatat ttaaccggaa tagttcgaga caagcaacgt cgaaaaatgt ctaagtcatt
     5581 aggaaattca cctgatccgt tagatttgat tgagaaatat ggtgctgatg gtgttcgggt
     5641 tggcttatta ctaagtgctt ctaccggaaa cgatattatg tttgatgaag aattatgcaa
     5701 taatggaaaa gctttcgcta ataaaatatg gaacgccttc aaattgataa atggttggga
     5761 agtaagtgat tctatttcgc aaccggaaag ttcaaaaatt gcaattgctt ggtatgaagc
     5821 aaaattagaa aaaacgttag cggaaattga agatcagttt gaaaaatatc gtttgtcaga
     5881 tgcattaatg gcaatttata aattggtttg ggatgatttc tgttcatggt ttttggaaat
     5941 gattaaaccg agttatcaac aaccaattga taaagtaacc tatactagag ctattgcagc
     6001 gttagaagcc aatttaaaat tgttacatcc gtttatgcct tttttgacag aagaaatttg
     6061 gcaccattta gcagaacgct caccggagca agccttaata atttcagaat ggccaactat
     6121 agcgaataca aatgttaatt tgattgctga ttttgaattt gcttctgaag tgattgcagg
     6181 tttgagaaca attcgtaaag acaaaaatat tccatttaaa gaagccatcg aattgaaggt
     6241 tatcaatcat gaaaatgcct caaaagagtt tgattcagtt atagttaaat taggaaatgt
     6301 ttcttccata gattacattt ctgaacaaga aaatggagct ttaacttata gagtaaaatc
     6361 caatgaatat tttattccga ttagtggtgc aattaatgta gaagaagaag tagcgaaatt
     6421 gcaggaggaa ttaaaataca cgaatggttt tttaaattca gtaagagcaa aactttctaa
     6481 tgaaaaattt gtatcgggtg ctcctgaaaa agttatcgaa atggaacgca aaaaagaagc
     6541 cgatgcttta gctaaaatag ctacaatcga gcaaagttta gcaagtttaa aatagtataa
     6601 aaaaagtata aaccaaaaaa gtccctattt ttagggactt tttattgtta taataatagc
     6661 ctgtaattta tttaacggct gtagcggttg tatatttctc tgctacttta tcccaattga
     6721 ttacattgaa aaatccttct aagtaatctt ttcgtttgtt ttggtgtttc aaataatagg
     6781 catgttccca aacatctaca tttaaaatgg gggttccggt atttcctaat tttgtcatta
     6841 aaggattatc ttgattagca gttgaaataa ttgttagttt accggtttta tccaaaatca
     6901 accaagccca tcctgaaccg aattgtttca tacctgcttc aataaattgt gttttaaaat
     6961 cagcaaaact gccaaaatct ttggttatcg cttcagcaag tgttccttta ggttctccac
     7021 ctttattggc tcccataatt tcccaaaaga agttatgatt gtaataacca ccaccattgt
     7081 tacgaacagc tttgttttca ggatctaatt ttttaaagat atcttcaatg gacatatttg
     7141 ccatttcagt tcctgcgata gctttgttca aattattagc atatgctaaa tggtgtttag
     7201 aataatgaat ttccattgtt ttagcgtcaa tataaggctc taaagcatta taagcgtagg
     7261 gaagggctac catttcaaaa gcaccgcctt catcaacttt tacatcagca ggattcccca
     7321 tttcagttac tactacttcc ttttcaggaa gtggaacttc cacaacttct tctaattttt
     7381 tgttattatt acaactaata agtgttgtga taacaagggc agaaagaatt acattttttt
     7441 tcatgagatt attttttatt taacaatgaa ttgattgttt cgatataaag ttctttggct
     7501 tcatcggatg aaatgtgact tatttgcatc caagcattgg ttttaaatgc atttcgcaaa
     7561 tcgtttgaag agggttgtgt gtagcccacc ataccatgag ttgcctgttt gtagtaagca
     7621 taaatacgca acatcacgtc cggaggtaat gtttcggtca tgttagaagc tttttcgaat
     7681 gcttctcgaa atcgagtatc taaatctttt tcaaccatga attatttttc agcaactacc
     7741 gtttctcctc cttttacagt ttgatttagc gttaccttaa tttttgtacc taaaggtaag
     7801 aataaatcca cacgtgaacc aaattttata aatccggcat ctgttccttg aactacttgc
     7861 attccttctt gtgcataatt tacaattcta cgtgctaaag caccggcaat ttgacgatat
     7921 aaaatttcac caaaaattct attttcaata acgacagtag ttctttcgtt ttcggtacta
     7981 gcttttggat gccacgcgac taagtattta ccgggatgat atttactgaa attaattttt
     8041 ccgtttgagg catatcttgt aacatgaaca ttgatgggag acatgaaaat ggaaacttgt
     8101 aaacgtttat ctttaaaata ctccggttca aaaacctctt caataactac tacttttcca
     8161 tcaaccgggg cgataatgtg gttatcatca ataggtgtat ttcgttttgg atttctgaaa
     8221 aattgtaaaa tgagaattag aaatagaagc ccaatagttt gaactgtcat ttttaaccaa
     8281 actaacgaaa tgaatgcatc tgccaataga atccaagcag tgactataac tgttgcaatt
     8341 aaaataattt tagctccttc tttatgaaac ataatttaaa atttggtaaa acaaaaaaat
     8401 gaatggtgca acaaatatca tactgtccaa gcgatctaat ataccaccgt gtcctggcat
     8461 gatttttcca ctgtctttta cacctgctat tcgcttaaat ctcgactgaa ttaaatcacc
     8521 caacgttgca aatatactaa ctaaaacggc tgttccaatc caaatggtta aattggaagt
     8581 atttaataaa tattttgcaa taataatacc ggcaataata gtaaacacaa aaccaccaat
     8641 gaatccttcg atagtttttt taggagaaat tcgttcatat aatttgtgtt taccaattga
     8701 ttttcctacc acataagcaa aagtgtcatt ggtccaaatt aaaacaaaaa tcccaatgat
     8761 aattttagga ttgtaccctg atagtccgaa aggaattttg gtgataatta caaagggaaa
     8821 aattatgtaa ccaattaaat aaagccactt ttcaaaattg gtaatcgttt tttctttagt
     8881 tgaaaataga gaatacaata atttgaagga gaccaaaacc gtgaaacctg ttataattcc
     8941 atcttgaaag ggataaaatg tagaatcaca aaatagtgca aatagtgcta ctgcgaaagc
     9001 tgctggaata cttttaggaa gatctactaa cttacagaat tcaaaagttg ttaaacccaa
     9061 aaaaccacca aacaatatat aaaaagatag ttctgaatgt aaaatacagc caactagtaa
     9121 aacaatataa actgaacccg ataaagctct tgtaaccgtt tcattcattt tataagtctt
     9181 ctaaaagcaa taagtacagg tttttagctg cacttccata ttgaagaaaa tcttcttctt
     9241 ttgctttttc gaaatattta atagtagtga tattagttgg gtaatcttta acgtaacgtt
     9301 tttttatttc tcttaaacca tcacttttac tttctaaaat ttgactggtt gtagcaaaaa
     9361 caatcatatt tgcaggtagt tcgtgtggtt tgttttgttt tatttgattt gaagttaata
     9421 aaacagagcc ttcatctgct attagatttt cacaactggt caataaaaat tttgggttta
     9481 ccggattttt aaaattgagt ttattttcgt ctaataaatg gaaaagtttt ggttcaaaac
     9541 aaactgcttc acattcaaac caatcgtttt cttctaagat atttaagaat tgttctttaa
     9601 tttcttctaa gttatcacaa tataaaaact tacctccatt ttttttgaaa ttgaaagtaa
     9661 ataattcatc tacaggaatt ttgttatccg gattaaaagg actggcatct ttttccttat
     9721 tctcttctga agcctcgttt ccggaaccaa ataacttttt aaaaagactc atactatttt
     9781 taacaattgt actagttgta attgaaaacg ttcaaagata aaaaaaactc aattcaaatt
     9841 tcatattgaa ttaagttttt tctgtttttt cttaaaattg gttcgaaaag ctattcacca
     9901 acggaaacat gcgactcatc tccaaaaggt ctttttccaa aaatagtttc taaatcatct
     9961 ttgaaaataa cttctttttc aattaatatg tcagctaatt gaattaattt atctttatta
    10021 tcttccaaaa gtttgatagc acgttggtat tgactttcaa ttaaatttga aatttcttta
    10081 tcaatggtaa ccgcagtttc ttcagaataa ggtttagaaa aactatattc actttgaccg
    10141 gaagaatcgt aataagtaac atttcctaat ttttcattta aaccataaat ggttaccatt
    10201 gctcgagctt gtttagtcac cttttctaaa tcacttaaag ctcctgtaga aattctgttg
    10261 aagattactt tttcagcagc acgtccaccc atagtggcac acatttcgtc caacatttga
    10321 tccggacgaa caattaatcg ttcttcaggt aaataccaag ctgcacctaa actttgacca
    10381 cgaggcacaa tagttacttt taccaatgga gcagcatgtt ctagcatcca acttaccgta
    10441 gcatgaccgg cttcgtgagt agcaattgct cttttctcat ccggtgttac aattttgttt
    10501 ttcttttcta aaccgccaac aattctgtca acagcatcaa gaaaatcttg tttgtcaacg
    10561 gcttttttgt catgtctagc tgcaataaga gccgcttcat tacaaacatt tgctatatct
    10621 gcaccggaaa acccaggagt ttgttttgct aaaaattcag tatctaaacc ttcttgtttt
    10681 ttcaaaggag ctaaatgcac ttcaaatatt tccttacgtt ctttaatgtc cggtaaatca
    10741 acatatattt gacggtcaaa tcgaccggct ctcatcaagg ctttgtctaa aacatcagca
    10801 cgatttgttg cggctaatac aataacgttg gtattggttc caaaaccatc catttccgta
    10861 agcaattgat ttagtgtatt ttcacgttcg tcatttgaac cagacatgtt attctttcct
    10921 cgagctcttc cgacagcatc aatctcatca ataaagataa ttgcaggtga tttgtcttta
    10981 gcttgtttaa ataaatcacg tactctggaa gctccaacac ccacaaacat ttcaacaaaa
    11041 tcagaacctg aaagagagaa aaacggaact tttgcttcac cggctaccgc ttttgctaat
    11101 aatgttttac ctgttccggg aggaccaact aacaaagcac cttttggaat tttaccgcca
    11161 agcgtagtgt atttttccgg atttttaaga aaatcaacga tttcctgaac ttcttctttt
    11221 gctccttcta atccggctac atctttgaaa gttactttaa catcggtttt ttcatcaaat
    11281 agttttgctc ttgattttcc gatattaaaa atttgaccac cgccaccacc acctgatccg
    11341 cctgacatac gacgcataat gataatccag atgactacaa ttagtatgat tggtaataaa
    11401 ttaattagaa tacctgacca attacttttt actaaataat tataatcggc taatttattt
    11461 tcctttttag ctaattccag tttttcctga aataatttac cgtcagcaat gttaggtaaa
    11521 agataatgtg cacccgtatt ttcacgacct aatacatctt ttacaacacc tttatgcttt
    11581 tgatctttca gggcatcttt cttgatatag atttcagctt cactgatatt ttcgcctttg
    11641 ttgtaaacca atactttttc aatttcacca ttttcaaggt attcgttgaa ttttgagatg
    11701 gttaatttac caggttcctg aaaggaactt ccgccggaaa tgatactgat aaatataaaa
    11761 ataccggcaa ctataaaata aagtagccaa ggattaaatt taaacttatt tgaattcgga
    11821 gtagaattat tttctttcgc cattatatat ttttcttctt tagcaatttt taaattttat
    11881 ttggaatcgt tgtgattttt gcatcacccc ataatccttc aatattataa aattcacgaa
    11941 catgtttttg aaatacatga acaacaatgt ccacataatc cattaaaacc cattcggcat
    12001 tatctgcccc ttcaacatgc caaggcttgt cttttagttc tttggaaacc gttttttgaa
    12061 ccgagttaac gatagcgtta acttgtgtgt tagaagtacc attgcaaatt ataaaataat
    12121 cacatactgt gttttcaata tcgcgtaagt ctaatatatc gatgtcattt cctttaactt
    12181 cttcaattcc tttgatgatg ttgattaaca attcatcttt actaactttt ttattcgcca
    12241 tttactattt tataattttc gcaaagttat caaattttgt gtttattttt gatgcttaac
    12301 acaagtttaa aacaatttca gtataataat actttatgca tatcatcaaa ctaagtgcca
    12361 ctccttcgac aaacaactat ttaaagcaat tatactccca acaagtagtt gctaatttta
    12421 ctattgttgt agcatcaaat cagttgaaag gaaaggggca gatgggtact tcgtgggaat
    12481 cagaatctgg taaaaacctc acttttagta tattgctaaa agatttgatt gttgatgtaa
    12541 agcaattgtt tacgctaaat attttagttt cacttgcctt agttgaagtt ttggaaaggt
    12601 ataaaattcc taatgtgact gtcaaatggc caaacgacat tatgtcaggg aatttaaaat
    12661 taggtggtat tttaattgag aatagtatta aatcagatgg ggaagtgatt tcgatattag
    12721 gaatagggct taatataaat caattgtatt ttgaaaaagc tcccaatgca acttctttac
    12781 atctaatttc agggagaaat tataatttag atgatctttt gctggatttt tgcacttcta
    12841 ttaaacaaaa tttaacaatg atggaaaagg attcagaaaa aatatgggtc aattatcatc
    12901 aaaagttatt taaaaaagga attccgatgg tatttgaaga tgttcatgga attaaattta
    12961 tgggaataat tcaaaaagtt tccaatgatg gtttattaca tattctttta gaagatgatt
    13021 ctattgctaa atttggagta aaggaaatca aaatgctata ctaattacaa aaaaaccacg
    13081 cttaaacaaa cgtggctttt aatcaacaac taaactaaaa attattaaaa gcagtaactg
    13141 ttttcagcta ctaatttgga ggtaatggtt tcacgtaaac caacaatatt tggcatattg
    13201 gtgtattttg taaaacgacg taaacccatt agcnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnaccaaca atatttggca tattggtgta ttttgtaaaa cgacgtaaac
    13381 ccattagcat catgcgttgt tcgtcacctt cagcaaatga aataatactt tctttccctt
    13441 tttgagttac tatatctacg gctttgtaaa ggtataattt agccatggca atttgttctt
    13501 ttgctttttc ttctcctact tttttggcta gtttttcagt tcgtaaaact gtgctttctg
    13561 ccatgtagat ttcaattaac atatcagcag cagccattaa aagttgttgg tgagcatcta
    13621 aatccattcc aaatttttgt acagccgctc cggcaaccat taaaaaggct tttttcaatt
    13681 tgccaaccat ttctttttct tctgaaaata attccgaata atccggtgtg tcaaaggagg
    13741 gaatgcccat taaatcttct tgcactttca tggcaggacc cagtaaatcc acatgacctt
    13801 tcattgcttt tttgattagc attcccactg aaagcatgcg gttgatttcg tttgttcctt
    13861 cgtagatacg tgcaattcgg gcatctctcc aagcactttc cataggagta tcttcagaga
    13921 atcccattcc gccatagatt tgaatccctt catcggcaca attttgaaca tcttcagaaa
    13981 cagctacttt taaaatggaa cattcgatag caaattcttc cactcctttt aattccgctt
    14041 cttgatgaga agttccttcg gccacacgag catttattct gtcttcaatt gctttagcag
    14101 cacgataagt agcactttca ccggcatagg cagaagttgc catttctgct aatttatagc
    14161 gaattgctcc aaatgatgaa attggtgtgt tgaattggac tctttcattg gcataattaa
    14221 tggcattgga agtcactctt cgttgtgcgt ctaaacaagc agctgctaat ttgatacgac
    14281 ctacatttaa agcattcatc gcaattttga aaccttcacc acgtccggcc aacatatttt
    14341 caaccggaac tttagtgtct gaaaagaata cctgacgagt agaagaggca cgaattccta
    14401 atttatgttc ttcttcattc attgaaatgc cgtttgatgg gtcattttcc acaatgaaac
    14461 ctgtgatgtt tttgtcgtct tcaattcggg caaaaacgat gaacaaggaa caaaaaccgg
    14521 cattagaaat ccacattttt tgtccggtaa tgttgtaatg tgtgccgtct gctgataaaa
    14581 cagcttttgt ttttcctgaa ttggcatcgg atcctgcacc gggttctgtt aagcaataag
    14641 ctccaaacca ttctcctgac gctaattttg gaacgtattt tttcttttgt tcttctgttc
    14701 cgtaaagtgt aatgggcatt gttccaattc cggtgtgagc accaaatgca gttgaaaacg
    14761 aaccggttgc tcctgaaatg tagtcacaca ccaaacaggt atccacgaat cccataccca
    14821 ttccgctgta agcttccgga acggctacgc ttaaaaagcc catttctcct gcttttcgca
    14881 ttacttcttc cgtaaatgca aagtctttct tttcaaaacg gtttttgtta ggccaaattt
    14941 ctttgtctac aaattctttt accgaatcac gcatcatgat ttgctcttct gagaaatctt
    15001 cgggtgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncac atgcttacga
    15121 ttccgtattt gcttccccgt aaacgcattt cgtcaaataa ttgaacagat agttttgctc
    15181 ctgtacaacc tagtggatga cctaacgcga ttgctcctcc gttcacattc acgatgtcag
    15241 gatttaaccc taattcacga acaacggcta aggattgtga tgcgaaggct tcgtttagct
    15301 caattaagtc aatgtcattt aaggttaaac ctgcttgttt taatgctttc ggaatggctt
    15361 ttaccggacc gattcccata attcttggtt caactccggc tgaagcgaaa ttgactaatc
    15421 tggcgattgg ttttattcct aattcgttaa ccaattcttc gctcatgatt aaaacaaaag
    15481 cggcaccatc actcatttgc gatgaatttc ccgcagtcac gcttccatca gcggagaata
    15541 ccggttttaa ttttcctaat gcggctacat ttgtatcagc tcttggtcct tcgtcttttt
    15601 ttacgatata tgatttggtt tctttcttgc cgttttcatt gatgaacgtt tgttcaacag
    15661 tgatgggtac aatttgtttg tcaaattttc cttcggcttg tgcttttagt gctttttgat
    15721 gggaatgaaa ggcaaactca tcttggtctt cacgggaaac attgaattgt tttgcgactg
    15781 cttcagcggt taaacccatt ccccaatagt aatcttcgtt tccggaagcg gcaacggcat
    15841 aatcgggtgt tggtttgtag ccacccatcg gaataaaact catgctttca gctcctcctg
    15901 caataatgca atcggccatt ccggattgaa tctttgcggt tgccattccg atagtttcta
    15961 aaccggaagc acaataacga ttcacggtta ctcccggaac atcggttact tttaatccca
    16021 ttaaggagat taggcggcct acgtttaggc cttgttctgc ttcgggcatg gcatttccta
    16081 ccatcacatc gtcgatgcgt tttttgtcga aatcgggtag ttcattcatc ataaactgaa
    16141 tggtttctgc cgccaattca tccggtcttt tgaaacggaa tactcctttt ggtgcttttc
    16201 ctactgcggt tctatatgct tttacgatat atgctgtttt catnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnggttttc ctttagttaa catgaattgg atacgttcca
    16381 atgtttttct ttctgtgcaa agtgataaaa atgcttcacg ttcaatgtct aaaagatatt
    16441 gctcacttac caatgtgggt tctgataaat caccgcctgc cataacatag gcaagtttgt
    16501 tggcaatttt acggtcgtgc tcggagatgt atttaccggc ttgcatttga tcggttccga
    16561 ctaagaacat tcctaaggct tgttttccta atactttgat gtcttttctt tgaatgggtt
    16621 gtgtgtaacc ttgttctgcc atttgtaggg ccacttgttt ggctactgca atttgacggt
    16681 cgcggtttac aacaacgatg tcttttcctt tttgaaggat gccgtaatcg aacgcttcat
    16741 atgcggatgt ggctactttt gccattccga tggtgaggaa gtattcttgc agtacgttta
    16801 attcgacgtc gtttttgtgg aataaatcgg aggcacgaac agtcatttct tttgaaccgc
    16861 cacctccggg gattacacct acgccaaatt ctaccaaacc gatgtatgtt tctgcggctg
    16921 caactactct gtcagcgtgc atgctcattt cacaaccacc gccaagtgtc attccgtgtg
    16981 gagcgacaat tacggggatg gaggagtagc ggcaacgcat catggtgtct tgaaacattt
    17041 tgatggccat gtttaattcg tcgtattctt gttcgacggc catcatgaat atcattccga
    17101 tgtttgctcc tacggagaag tttgttcctt ggtttcctat aactaatccg ttgtattctt
    17161 tttctgctaa gtcaattgct ttgttgattc cttgtaatac tccgccgcca atggtgttca
    17221 tttttgagtg gaattcaagg tttaggattc cgtcgcctag gtgttggatg gttgcgtctg
    17281 catttcccca cactttgtgg ctttcccgaa tgttatttag gatgataaat gcatcttgac
    17341 cagggatttt ttcctgtgtt tttgatggga tgttatagaa atgtgttgct ccgtttttaa
    17401 tggtgtagaa ggagttgctg cctgctgcga gcatatcggt tacccatgtg gctggttcgt
    17461 agccttctgc tttcatgagt tcaattcctt tggctacgcc gatgctgtcc catatttcga
    17521 agggtccgtt ttcccaaccg aaaccggctt tcatggcatc gtctattttg tagagttcgt
    17581 ctgagatttc gggtattcta ttggaacagt aggcaaacat agcggcgaag ttctttctgt
    17641 aaaagtctcc tgctttgtct tttcctttga cgagtacacg gaaacggtcg atgggtttat
    17701 cgatggtttt agtgagttcg agtgttgcaa aggaggcttt cttgtttgga cggtattcta
    17761 acgtggtgag gtcaagggaa agaatgtctt tgtctacttt tttgtagaaa ccttgtcctg
    17821 ttttgcttcc aagccatttg ttttccatca ttttgtcgat gaaggaaggg agtttgaaga
    17881 ggctgtgtgc ctcatcggag atgcagtttt ggtatattcc gtttgcgacg tggactaagg
    17941 tgtctaaccc tactacatca acggttctga acgtggctga tttaggtcgg ccgatgacag
    18001 ggccggttaa tttgtctact tcttcgatgg tgagtcccat ttcttgtact aggtggaaaa
    18061 ggctcatgat gccgaagatt cctattctgt tgccgatgaa ggcaggggta tctttggcaa
    18121 cgactgatgt ctttccgaga aattgtgaac catagttggt taagaaatct aaaacggact
    18181 gacttgtttt tggtccgggg atgatttcga agagtttgag gtaacgggca gggttgaaaa
    18241 agtgcgttcc gcagaagtgt tgttgaaagt cttctgaacg gccgtcgctc atgaattgta
    18301 ttgggattcc ggaggtgtta gaggttacta atgtgccggg ttttctgtat ttgtctactt
    18361 gttcgaagac gagttttttg atgtcgaggc gttcaactac cacttcgatg atccagtcga
    18421 cggtggcgat tttcggcatg tcgtcggtgg tgtttccggt ggtgattctt tttgcaaact
    18481 gttggctgta aatgggtgag gggtttgacc ttaaggcatt ggttaaactg tcgttaacga
    18541 ttcggttgcg gacgggtttg ctgtctaagg tgagtccttt ttgttgttcg ggttcgttga
    18601 gtgcgtttgg aatgatatcg agcaggagta cttcgacacc gatgtttgca aaatggcaag
    18661 cgatgccgga acccatgatt ccggaaccga ttacggccac ttttttaata gttcttttca
    18721 tatttaaagt cttgttacga attgcaggtt gtgggttacc ggttttttcg ttgggttata
    18781 gttagaatat tttcttttcg gtgattagtt cgttgattat ttcagctact tccatgaagg
    18841 tgtttagttt gtcttcgggg atgttttgtt tgattgtttc attgaacttt atgacggtgt
    18901 ttttggataa ttctcttttt tctttgccga gttcggtgag gtagatgagg acgcctcgtc
    18961 cgtcgtcggg gttttttttg cggatgatta gttttttttc ttcgagtgtt ttgagggttc
    19021 tggtgaggct tgtggcttcc attcccattc tgttgctgat gtaggtggag ggggttccgt
    19081 cttctttgtc gatgctgagg agggcgaaac cgatggccat ggagccttcg tattttgagg
    19141 cttcatcgtt gtacatccgg gcgacagatt gccaggttgc tcgaaggata tagtctatgg
    19201 ttttgtcttt catgtttttc gcaaacgttt tcaaagatat ataaaattta ttatgcacgc
    19261 atagtatttt ttacttttta acactttttt tgttaagtag tgtttgaatt gttgtgtttt
    19321 gtttggtttt
//
LOCUS       DAOTCW010000037        19323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1545_length_19323_cov_28.273147, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000037 DAOTCW010000000
VERSION     DAOTCW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 19323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19323
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1545_length_19323_cov_28.273147"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="P5335_04725"
     CDS             complement(<1..1595)
                     /locus_tag="P5335_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRZ74216.1"
                     /translation="MKKVSAIICAYNEEKTIKDVIISVSKSRIIDEIIVVNDGSVDNT
                     QKIIKELKKEIEIRDIHFEENKGKGFAMAAGVEQAKFDIMLFIDADLSNIVSEHVNQL
                     INPLLNEEADMVLGQATETLINYSINPFKSFSGERALLKEDIIPVIEKMKTSRFGVET
                     LLNLYYQSLGKTVKYVMLKNLEHPTKFSKTSFLKATKEFLYEGHEIATTVIRNYDLIA
                     KTLKRIINKNMKIKYLIFAIFIALVGSVNLAKAQEDADKLKLSGELLTDQRFLYESPN
                     DWAWNENRLTLNLDKRMENSKFHSEVWLRNIGLPNITRSSGLYNKGIVDPYNLEIREA
                     YIQLYGFLTKNLDVTIGRQRIVWGTADKLNPTDNLNPLDLEDILDFGRRRGSDAINLN
                     YYLNNDFSLQGIFIPFFQPANMPIGIFANALNPSMALPQGMVLKGFSDTLMIPRYNLG
                     ESSTAGLKFKGFAKGVDFSLSYVWGYDGLPFATRNTFTPVDAFGGININSQLSFARTH
                     IIGADMATSIAGIGFWAEAAAFIP"
     gene            complement(1592..2494)
                     /locus_tag="P5335_04730"
     CDS             complement(1592..2494)
                     /locus_tag="P5335_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRZ74217.1"
                     /translation="MKQKVALVLGSGGARGTAHIGVIRELEKQGYEITSISGTSMGAL
                     VGGVYAAGKLDEYESWLRSLSKMDIFNLVDFTLSTNGIIKADKVLNEIQKFIPDHKIE
                     ELPIKYAAVVTDFKQKKEVVITEGSLFGAIRASISIPFVITPVLKSDTVFVDGGVLNP
                     VPVNRVARQRNDILIAVNVNAQIPYTKPIALNQDLGYFEKLTNGKLSEFQRRLTSFFP
                     ANTHKKENPGVGYFNLISDTSSLMLSQIAKLTLEMNPPDILIEVSRDSCGTFDFYKAE
                     ELIELGKKATREGMGKLINQKSLS"
     gene            complement(2498..3046)
                     /locus_tag="P5335_04735"
     CDS             complement(2498..3046)
                     /locus_tag="P5335_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRZ74218.1"
                     /translation="MKIIYRYILVNLLGWKIKGEFPNIKKSIIIFAPHTSYWDGLYGK
                     LYLMQLGINYKFLSKKEFFKFPLKYFFIIFGSIPVYENKKYIDYVVGLFNNSKELHLV
                     LSPEGHLAKVTRWKKGYFYMAVKADVPIVVSYLDYKKKEIGIKGVIYNSTNIGAVRKE
                     IAELYSDVTAKYPEKFSHEIKI"
     gene            complement(3074..3898)
                     /locus_tag="P5335_04740"
     CDS             complement(3074..3898)
                     /locus_tag="P5335_04740"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRZ74219.1"
                     /translation="MASNLHTFNGKVVIITGVSSGIGKACAFEFADNGATVVLAARRE
                     NELKKVETEIKIRGGEAYAVKTDVKNIDACKNLIDKTVEKYGKIDVLINNAGVSMRAT
                     FEELNLSVIKELMDTNFYGAVYCTKFALPFLLEQKGTLIGISSISGLTPLPGRTGYCA
                     SKYAMEGFFSTLRLENIKKGLNVLVVRPGFTSSNIRKAAFNKLGVPQNETPRDESKMM
                     SSKEVAEIILKAAVNRKRNLTLTLHGKLTVWLHKIFSGITDRIILYEMAKEPNSPF"
     gene            complement(4055..4822)
                     /locus_tag="P5335_04745"
     CDS             complement(4055..4822)
                     /locus_tag="P5335_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRZ74220.1"
                     /translation="MKEKLKNIFFKFWYWYLSFIDKNADIIFMNYGYSKDNNKIELEE
                     TDEKNRYSIQLYHLVATGVDIKGKDILEVGCGRGGGLSYINRYLSPNFVTGVDLNKKA
                     INFCNKKYSTDRVKFLQANAQKLNFQKDSFDAVINIESSHRYSRIDLFIDEVYRILKP
                     GGFFLFADFGDNMEIEKLKTQFVKSNLKIVRFENITDNVIEALKLATPDREELIREIL
                     PKFFQNLGRNFAATEGTTTYNKFLTREFEYVFYALMK"
     gene            complement(4842..5084)
                     /locus_tag="P5335_04750"
     CDS             complement(4842..5084)
                     /locus_tag="P5335_04750"
                     /inference="COORDINATES: protein motif:HMM:NF002150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRZ74221.1"
                     /translation="MNHQEIKKIVTKIVSRKLKISMDEISPDSTLKDLGADSLDELEV
                     ILELEQKLDITLPDEITPELQTIGNLCKFIEKRFES"
     gene            complement(5112..5849)
                     /locus_tag="P5335_04755"
     CDS             complement(5112..5849)
                     /locus_tag="P5335_04755"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRZ74222.1"
                     /translation="MKSRFQTAVITAIIVAGGVFSANAQDASTILKKMDDVMYSPKDM
                     IGNNKIILIDKNGKEETREATVQQKGNDKRMFRFTSPASQAGISVLSLPNDVMYLYLP
                     AFGKERRIATSAKNQNFAGTDFSYDDMESVLFSVKYTPKLVKTEDNVFVLELTPVGGK
                     SDYSKVMVQVNKTDYYPELMEYYDKGNTKVKEAKYTFKKIGNYWNAAEIEMTDLKKNH
                     KTKMQMSDVKYDMGLTDDDFTVRKLKQ"
     gene            complement(5868..8126)
                     /locus_tag="P5335_04760"
     CDS             complement(5868..8126)
                     /locus_tag="P5335_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRZ74223.1"
                     /translation="MNRFADGIVKFKWLIIIAVVGLTVFFSYQAKDLHINADIISSLP
                     DDDPVARLYKEIGTQFGGNDMGMIVLETDDVFKAEVIQHIKQITDSIKYTNGVSTVTS
                     LTNILDIKSSEWGIEIGKLVDEYDLPVEQSQLDSLKNYVFSKEMYRGAVVSEDGTATA
                     IMFTLLPEGDKQAVAKEIKDKIEALDLPETTYFGGLPMMMNDINNLIISDMVWLIPIV
                     FIVIAIILLLSFKSFRGVLLPLLTAGLAVIWTIGLMTVTGYELTIISNIIPVVLLAVG
                     SAYTIHVLNSINHYKLTDRKKALIQSLGYIMVPVILAAVTTAIGFVSFVFGAYLTMIK
                     DFGIFTAVGTLIALMLSLFFVPALISGLSMYSKKNVIENKEKQTILTHKILLPLVNFL
                     FKHPKYTLTGWGVLLMLCIGGITLIKTSVNMADYFKKDNPTRVAEDVMQKKFGGSLPV
                     FIVFEGDMQSPEVLKMMISTEDFMKEDPNIQVTQSVADLIEQMNDAMGEGKKIPDDKA
                     KIEQLWFLLDGQEVMPQLVNNDLSKGIIQSKFASVDSKELEVFTAKMNRFIEDNPNEH
                     CKIELTGMPSVYVKLNSSLINSQYNSLLIAIIMVVLIVGLILRSGTKGIFAAIPVVAT
                     IIVLFGFMGITGIPLDIATVLVASIALGIGIDYSIHVITGFNKHLITHGDAEKAIEET
                     ILLSGKAVIINVISVSAGFLVLLFSQIVPLQNFGILVAISMFGSGIGALTLLPIILIL
                     ANRKRKIIANNH"
     gene            complement(8191..8772)
                     /locus_tag="P5335_04765"
     CDS             complement(8191..8772)
                     /locus_tag="P5335_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRZ74224.1"
                     /translation="MSIDNTKESILSVANKLFSRFGFHKTSMDEIAKIARKAKGSLYY
                     HFASKEDLFREVVSIEMINLKNQLSFIVNNPDLTSTEKVKKYLVKRMEILNSAANYHE
                     TLKADFFEHFHFIDDLRTELDAWEKENLKKLILQGVERGEFAIIGDIDVLLDVFIMVI
                     KGLEIPFFLQNKYVKYAPHFEGLIGILTKGLSR"
     gene            complement(9575..10180)
                     /locus_tag="P5335_04770"
     CDS             complement(9575..10180)
                     /locus_tag="P5335_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRZ74225.1"
                     /translation="MTDKKTNIKNSTFNLFKKYGIKRITIDDIAFELGVSKKTIYQFY
                     ETKNDLVLTVFSDLEEDINKSSIDSIFKENRSPIEEIILLFNTKFKLTKEFNPVLLYD
                     LSKYYPAIYRNLKTSISNNLYIKCKENINKGIYDGVYRKDLNPENVSRLFLHHIFGFH
                     ESAFIDRSMLTNKTFIHEIIAYHIRAIATQKGIKIFDKLRE"
     gene            complement(10204..10497)
                     /locus_tag="P5335_04775"
     CDS             complement(10204..10497)
                     /locus_tag="P5335_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74226.1"
                     /translation="MKAVLITYNQSITEEVQLILDKLAIRGYSKWTGITGRGTSQGEQ
                     HDGTHTWPELNNAHITIVNDEKVSLILERLKDLNEEVEEQGLRAFVWNIEEMI"
     gene            complement(10494..13631)
                     /locus_tag="P5335_04780"
     CDS             complement(10494..13631)
                     /locus_tag="P5335_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRZ74227.1"
                     /translation="MSIYSTSVNKPVSTIMIFIGVVIFGLFSYINLPVDYFPKIDPPI
                     ISVFTFYQGANAADVEQNITRKLEDGFGSLTGLKKISSNSKDNISIITIEFEWGTNLD
                     EATNEVRNAVGMAERNLPDEVETPTIFKISTSMVPVLMYSVTSNESYAGIKDIVDKKI
                     VQQLNRIDGIGNIIQMGAPVRAVMVDVDPRKLDAYNLTVEQIAGILGANNLNLPSGNL
                     EMGKSDLPLRLQGEFESSDIIKNIIVSNINGKTVYLKDIANVCDSLKDVKSYERANGE
                     KNVRIMIQKQSDANTVTVADKIKKKMAEIDKTLPSDVKVEVLMDSSVNTINSINNLAE
                     TLIFALVFVVLVVIMFLGRSRPTFIISLSIPISLIAGIIYMYLSGGTINIITLSSLSI
                     AIGLVVDDSIVVLENITKKLERGGFSRESAIYGTSEVSLAVIASTLTIIAVFLPLTML
                     GGITGILFKPLGWVVTISVATSVIVSMTLVPMLSSKLLDKELPNKNTFRGKIYWFSQN
                     ILERIDNFYSKTLEWAVGHRWVVIGIATVVFVVSLFLVKLIGSEFMPASDNDRISAQV
                     KLAQGTKLEETVKTAHYLDSLFRAKYSEIEVVSTSAGAGDGNSLIALFTENGNYIVNY
                     TFKMKPLKERNRNIFQIADEMRKDIEVLPEVEKFYVDPGSSRASSAMGMGGGNNLDVK
                     IYGNNFDETNIVAEKIYNTLKNINGTKDILISRDREKPELQLVLDNAKMTSFGLTTAQ
                     VAMAIRNRINGVTATKYREEGNEYDVIVRYDKKFRESTEDIANITIMTPMGKMIKLSE
                     ITTLKRFYSPPNIERENKVRMVKVSSALSGIDVGTLQANIEAEIKKMDIPRDVTIEMG
                     GSAENMKDSFRDLALLIVLSLILVYIVMASQFESLTEPFIIMFSVPFAFTGVFLGLFI
                     FNSTINVISLIGVVMLIGIVVKNGIILVDYTNLLVDRGDSLKNAVVKAGRSRLRPVVM
                     TSLTMILAMIPMIVAKGTGSEMWRPMAISIFSGLTFSTIVTLILIPTIYTIFGVGKIK
                     RQRKEAIKSLKKITK"
     gene            complement(13668..14720)
                     /locus_tag="P5335_04785"
     CDS             complement(13668..14720)
                     /locus_tag="P5335_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRZ74228.1"
                     /translation="MNALKLLSGCIISGIIVVFSSCTSKSDKTKTENQDSLKLVSVKS
                     MVLKQEQVNQNIDYTATLIPFEEVHLAPASPGRIEKINVEISDNVTKGQIVALMDRTN
                     LETARLNMMKLETDYKRLDTLKKTKSIADQQYDQIKSAYEIAKNSYQFLLDNTQLKAP
                     FSGVVSGKYFEDGEIYSGTPVSSVGKPAIISIVQINQLKALVGISSNYFPLINVGMKA
                     NIKSELYPDMKFDGQIIKIYPTVDNVTKTFILEVVIQNANLKLRPGMFAKIQLNLGKG
                     SAILVPSIALIKQTGTNDIYLFVNKNNVAIKTLVKTGKMFDDKIEILEGVNEGDEIIV
                     VGQNKLENQTPISVIK"
     gene            complement(14732..16033)
                     /locus_tag="P5335_04790"
     CDS             complement(14732..16033)
                     /locus_tag="P5335_04790"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRZ74229.1"
                     /translation="MNDYLKITICCILILCSNVLKAQETLTLSLDSSIVYAIEHNKIL
                     ISSKLTIDKSSQKIKETISQGLPQINASVDYNNFLGAEAEFKISEMAPPAVIEFNPTS
                     NAKLSVSQLVFSGSYYVGIQLSKLAKTITEQSYQKDELNVKEQTIQAYYMILVSEKLL
                     KIRLENKNNIQLIYEKTNNLANTGIIELTEPKKLLIIITSIDNAIKATERQVEMGYNL
                     LRLQLGLSSEQEIKLTTDLEMLEQKFVTQSLMDDTFNIENNMDYKLISMQGDIAQKNI
                     KLKRAAYLPTLAAYYSYTEKLIKPKFDLSPNQVVGLSLNIPIFSSGFRKYQVNQAKTD
                     YYISLNNKELLTDQLTLQEKQLRYNYKNLYEQFQNQKANVEIAKEVFDKMNLKYEQGI
                     VSSLELTSANNEYLNAESAYAGILLQLLNAELSLRKINSNF"
     gene            complement(16111..16554)
                     /locus_tag="P5335_04795"
     CDS             complement(16111..16554)
                     /locus_tag="P5335_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRZ74230.1"
                     /translation="MEKRYIDFREIGKLKKEFFRVLLHIAKEKTNIHLTVEQLGLLYL
                     MHIKDFDVIQKQLAVYTGKNKSTILRITDSLEEKGLVRKAEDKNDRRKNYLMITKTGQ
                     TTIEVYLKIMDEIVDDLQKGINETDLKVFSKVIDHIKVRVANYEV"
     gene            complement(17523..19109)
                     /locus_tag="P5335_04800"
     CDS             complement(17523..19109)
                     /locus_tag="P5335_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRZ74231.1"
                     /translation="MLILQNISYTHPSKDLLFSDINLTVNNHEKTALIGNNGAGKSTL
                     LKIIAKELQPSNGQIIVETEPYFIPQIFGQFNHLTIAEALKIDKKLNALKEILNGNTT
                     EENFNLLNDDWTIEDRCNDALQHWQLIDIDLSQKMEALSGGQKTKVFLAGISIHQPEL
                     ILLDEPSNHLDIASRKLLYGFIQTTKSTLIVVSHDRKLLNLLDQVCELSKHEIKVYGG
                     NYDFYKEQKQIENNALSQDIQSKEKALRKAKEKERETLERQQKLDNRGKGKQEKAGVA
                     RIMMNTLRNNAENSTSKLKSTHAEKIGGISQDLQELRSSLPDIDKMKFGFDNSALHKG
                     KILFTATNINFAYNMQPLWTENLNFQITSGERIALKGQNGSGKTTLIKLILGDIEPQT
                     GTIYRANNKSVYIDQDYSLLDNKLKVYEQAQQFNVSALQEHEIKIRLNRFLFTKEDWD
                     KSCSALSGGERMRLLLCCLTINSKSPDIIIFDEPTNNLDIQNVEILTTAINEYQGTLI
                     VVSHDETFLEQINIERTIEL"
BASE COUNT         5690 a         3577 c         3061 g         6995 t
ORIGIN      
        1 ggaataaatg ctgctgcctc ggcccaaaaa ccaatacctg caatgcttgt agccatatca
       61 gcacctatta tatgcgttct tgcaaacgat agttgagaat tgatattaat tccgccaaac
      121 gcatcaacag gagtaaaagt atttcttgtg gcgaaaggta atccgtcata tccccaaaca
      181 taactcagag aaaagtcaac acccttagca aaacctttaa attttaaacc cgcagttgaa
      241 ctttcgccca aattatatcg aggtatcatt aaagtatcag agaaaccttt taaaaccatt
      301 ccttgaggta atgccataga cggatttaaa gcatttgcaa aaataccaat cggcatattc
      361 gcaggttgga aaaaagggat aaaaatccct tgaagcgaga agtcgttgtt taagtagtaa
      421 tttaggttaa tagcgtcaga accacgacgg cgaccaaaat caagaatatc ctccaaatcc
      481 aacggattaa gattgtcggt tggatttaat ttatcggcag ttccccaaac aatgcgttga
      541 cgaccaatgg ttacatctaa atttttagta agaaaaccgt atagctgaat atacgcttct
      601 ctaatctcca aattataggg gtcaacaatt cctttgttgt acaagccact tgaccttgta
      661 atatttggca aaccaatatt tctcagccac acctcgctat ggaattttga gttttccatt
      721 ctcttgtcga ggtttaacgt taggcgattc tcattccaag cccaatcgtt tggcgattca
      781 tataaaaaac gttggtcggt caataattcg cctgatagtt ttaatttatc ggcatcttct
      841 tgcgcttttg caaggtttac actgccaact aatgcaataa atattgcgaa gattaaatat
      901 tttattttca tatttttatt gattattctt ttaagtgttt ttgctattaa atcgtagttt
      961 ctaattactg ttgtggctat ttcatgtcct tcatataaaa attcttttgt tgcttttaaa
     1021 aaggatgttt tgctgaattt tgtcgggtgt tcaagatttt tcagcatcac atattttaca
     1081 gttttaccca acgactgata atataagtta agcagtgttt caaccccaaa cctcgaagtt
     1141 ttcatttttt caataacagg tatgatatct tcttttaata atgccctttc gcccgaaaat
     1201 gatttaaaag ggttaatact gtaattaatt aaggtttcgg ttgcctgccc aaggaccata
     1261 tctgcttctt cattcaataa aggatttatt aattgattaa catgttctga aacgatattt
     1321 gacaggtcag catctataaa caacataata tcaaattttg cctgttcaac acccgcagcc
     1381 attgcaaaac cttttccttt attttcttca aaatgaatat cccgtatttc aatttctttt
     1441 ttcagttctt taattatctt ttgagtatta tcaacagagc catcgtttac aacaattatc
     1501 tcatcaatta ttcttgactt ggaaacagaa attattacat ctttaatcgt tttttcttcg
     1561 ttgtaagcac aaattattgc tgatactttt ttcatgacaa agatttttga ttaattaatt
     1621 ttcccatgcc ttcccgggta gcttttttac ctaactcaat aagttcctcg gctttataga
     1681 aatcaaacgt gccgcacgaa tcccttgaaa cttcgataag tatatcgggt gggttcattt
     1741 cgagggttaa ctttgcaatt tgcgataaca taagacttga tgtgtcgcta atgaggttaa
     1801 aatagcctac cccggggttt tcttttttat gcgtatttgc cgggaaaaaa ctggtcagcc
     1861 tcctttgaaa ctcacttagt ttaccgttgg tgagtttttc gaaatacccc aaatcctgat
     1921 tcaaggctat tggctttgta tatgggattt gtgcatttac attaaccgcg ataagaatat
     1981 catttctctg ccgggcaacg cggttgacag gcacggggtt aagaacacca ccatcaacaa
     2041 aaacggtatc actttttaac acaggtgtga taacaaaggg aatagatatt gaagcacgta
     2101 ttgcgccaaa taaactacct tcggttatca ccacttcttt tttctgttta aaatcggtaa
     2161 ccactgctgc atatttaatg ggtaattcct cgattttatg gtcgggaata aatttttgta
     2221 tctcatttaa aaccttgtca gctttaatta tcccgtttgt gcttagtgta aaatcaacca
     2281 ggttgaatat atccattttg cttaatgagc gcagccatga ttcatattca tccagttttc
     2341 ctgcggcata aacacccccc accaaagccc ccatagaagt accggaaatg gaagttattt
     2401 cgtaaccttg tttttcaagt tcacggatta ccccaatatg tgcggttccg cgagcgccgc
     2461 cacttcctaa tactaatgct actttttgtt tcatgtccta tatttttatt tcatgtgaaa
     2521 atttttcagg atattttgct gtaacatcag aatatagttc agcaatctct tttctaactg
     2581 caccaatatt tgttgaattg taaatcactc ctttaatccc gatttctttc ttcttataat
     2641 caagataact aactactatt gggacatctg cttttactgc catatagaaa tatccttttt
     2701 tccaacgtgt aactttagcc aaatgtcctt ccggcgataa cactaaatgt aattccttgc
     2761 tattgttaaa taatccgaca acataatcaa tatatttttt gttttcataa actggtatcg
     2821 aaccaaagat tatgaaaaag tatttcaatg gaaatttgaa aaattctttt tttgatagaa
     2881 atttgtaatt tatacctaac tgcatcaagt aaagttttcc atacaatcca tcccagtagc
     2941 ttgtgtgtgg ggcaaaaatt attatagatt tctttatgtt tggaaactca ccttttattt
     3001 tccatcccaa caaattaact agaatatatc tgtaaattat tttcatacaa gagcgcttat
     3061 ttgtttaaaa atttcaaaag ggagaattgg gttcttttgc catttcatat aaaattattc
     3121 tatcagtaat gcctgaaaaa attttatgta accaaacggt aagtttccca tgtagagtca
     3181 atgtcagatt tctctttcgg tttacagctg cttttaggat aatttctgcg acttctttgg
     3241 aagacatcat tttactttca tctcttggag tttcattttg cggtactccg agtttgttaa
     3301 aagcggcttt ccttatgttt gacgatgtaa agccaggacg aacaaccaaa acattcaatc
     3361 ctttcttaat gttttcaagt cgtagcgtgg aaaaaaaacc ttccattgca tattttgatg
     3421 cgcagtagcc agttcgtccc ggtaagggtg ttagccccga aatggatgaa attccaatta
     3481 gtgttccttt ttgttcaagc aaaaagggta aagcaaactt tgtacagtaa acagcaccat
     3541 aaaaatttgt atccattaat tctttaataa cgctcaggtt taattcttca aaagttgccc
     3601 tcatagatac acctgcatta ttgataagca catctatttt cccatatttt tcaacggttt
     3661 tatcgattaa atttttacag gcatcaatat ttttgacatc tgtttttacg gcgtacgctt
     3721 cacctccccg tattttgatt tctgtttcaa ctttttttaa ttcattttct cgtctagcag
     3781 caagaactac cgttgctcca ttatcggcaa actcaaaagc acatgctttt cctattccag
     3841 aagacactcc ggttataata acaactttac cattgaatgt atgtaagtta ctagccattt
     3901 ttacaattgt ttgatatagc acctggttcg tttgtgcttc ctgtattcaa acttattcca
     3961 cagattatta atttttacat tattttccat ttcacgtgtt gactcgaagt tattaattcc
     4021 attatcgttt tatagtcagt ttgcagtaaa aaaatcactt catcagtgca taaaaaacat
     4081 attcaaattc tcttgtcaaa aatttattgt atgttgttgt cccttcagtt gcagcaaaat
     4141 tccgccccaa attttgaaag aacttaggta atatctctct tattaactct tctctatctg
     4201 gtgttgctaa tttcaatgct tcaataacat tatcggtaat attttcaaat ctgactattt
     4261 tcaaatttga cttcacaaac tgagttttaa gtttttcaat ctccatatta tccccaaaat
     4321 cagcaaagag gaaaaaaccg ccgggtttca aaatacgata tacttcatct atgaataaat
     4381 ctatacgaga atagcggtgt gaagactcaa tatttattac tgcatcaaat gaatcttttt
     4441 ggaagtttag tttttgtgca tttgcctgta aaaatttaac tctgtctgtt gaatattttt
     4501 tattacaaaa gtttattgct tttttattca aatcaacacc ggttacaaag tttggtgaca
     4561 aataacgatt tatatacgat aagccaccac cacgcccgca acctacttcc aaaatatctt
     4621 tccctttaat atctacacct gttgcaacca aatgataaag ttgtattgaa tacctattct
     4681 tttcatcagt ttcttcaagt tcaattttat tattatcttt tgaataacca tagttcataa
     4741 aaataatatc tgcattttta tcaataaaac ttaaatacca gtaccagaat ttaaaaaaga
     4801 tattttttag tttttctttc atagaaataa cgttttgttg attacgactc aaatcttttt
     4861 tcaataaact tgcacagatt tccgatggtt tgcagctctg gcgttatctc gtcgggaagg
     4921 gtaatatcaa gtttctgttc aagttcaaga attacttcaa gttcatcaag gctgtcggca
     4981 cctaagtctt tcaaagtaga gtcagggctg atttcatcca tagaaatttt caattttctt
     5041 gacacaattt ttgttactat tttttttatt tcctggtgat tcattttgcg taaaccgata
     5101 ttgaaatttt attattgctt taattttctg acagtaaaat catcgtctgt caaccccatg
     5161 tcgtatttca catccgacat ttgcatcttg gttttgtgat ttttctttaa gtcggtcatt
     5221 tcaatttcag cggcattcca atagttgcca atctttttga aagtgtattt ggcttctttt
     5281 acttttgtat tgcctttgtc gtaatactcc attaattcag gataataatc tgttttattt
     5341 acctgtacca ttacttttga ataatccgat ttaccaccga caggagttaa ttccaataca
     5401 aatacattgt cttcagtttt cactaatttt ggggtgtact ttaccgagaa tagtacagat
     5461 tccatatcgt cataagaaaa atcggtacct gcaaagtttt gattttttgc gctggtcgca
     5521 attctccttt cttttccaaa tgcaggtaaa tacaaataca tcacatcgtt tggtaacgat
     5581 aaaacggata ttcctgcctg cgatgccggt gaggtgaatc gaaacatgcg tttatcgttg
     5641 cctttctgtt gtacagtggc ttcacgggtt tcctctttcc cgtttttgtc aatcaggata
     5701 attttattat tccctatcat gtctttgggc gagtacatta cgtcatccat ttttttaagg
     5761 atggtacttg cgtcttgcgc atttgcagaa aaaacacctc ctgctacgat aatggcggtt
     5821 attaccgctg tttgaaatct tgatttcata ctatttattt ttaattgtta atgattattt
     5881 gctattattt tgcgttttct atttgccaat atcaatataa taggcaataa agttaaagcc
     5941 ccgataccgg agccgaacat acttatggca accagaatgc cgaagttttg caatggaact
     6001 atttgtgaaa aaagaagcac taaaaatcct gctgataccg atataacatt gataattaca
     6061 gctttaccac taagcaatat ggtttcttct atagcttttt cagcatctcc gtgagttatc
     6121 aaatgcttat tgaaaccggt aattacatgg atagaataat cgatgccaat acctaaagca
     6181 atgctggcca caagtacggt agcaatatca agtgggatac cggttatgcc cataaacccg
     6241 aataacacaa taatagtggc aacaacgggt atggcggcaa aaatcccttt tgtaccggaa
     6301 cgtaaaatca agcccacaat aagtacaacc ataataatgg ctatcaacaa actgttatac
     6361 tggctattta tcagactgct attgagctta acatacacag acggcatgcc tgtcaattca
     6421 attttacaat gttcgtttgg gttatcctcg atgaaccggt tcatttttgc agtaaacact
     6481 tcaagttctt tactatcaac cgatgcaaat ttcgattgga taattccttt ggataggtca
     6541 ttgttcacga gttggggcat aacctcctgc ccatcgagca gaaaccataa ttgttcaatt
     6601 tttgccttgt cgtctggaat ttttttaccc tcgcccatgg catcgttcat ctgttcaatc
     6661 aagtctgcta ccgactgtgt tacttgaata ttcgggtctt ccttcatgaa atcttcggta
     6721 ctaatcatca ttttcagcac ctcgggactt tgcatatctc cttcaaaaac tataaatacc
     6781 ggtagcgagc caccaaattt tttctgcata acatcttctg caacccttgt aggattatct
     6841 ttcttaaaat aatcggccat gttaacgctg gtttttatca acgttatgcc accaatgcat
     6901 aacattagca gaactcccca acctgtaagt gtatatttgg gatgtttaaa cagaaaattt
     6961 actaagggca gcaaaatttt gtgggttaat atggtttgct tttctttgtt ttcgattacg
     7021 ttttttttgc tgtacattga taaacccgat ataagtgcgg gaacaaaaaa caaggatagc
     7081 attaaagcga ttagtgtacc taccgcagta aatatcccga aatcttttat catggtgagg
     7141 tatgccccaa aaacaaacga tacgaaacct atggccgtgg ttactgctgc aagtattaca
     7201 ggaaccatga tatatccaag tgattgaatt aatgcctttt ttctgtcggt aagtttatag
     7261 tggttaatac tattgagcac atgaattgta taagcacttc ctacagcaag caacacaaca
     7321 ggaattatat ttgaaataat ggtcagctca tatccggtta ctgtcattaa ccctattgtc
     7381 catataacgg ccaggcctgc ggtgagcaac ggcaacaata cccctctaaa agatttgaaa
     7441 ctcaacaata aaataatggc tattacgatg aaaacgatgg gaataagcca aaccatatcc
     7501 gaaatgatca aattgttaat gtcgttcatc atcatgggga gaccgccaaa ataagttgtc
     7561 tcgggcaaat ccaatgcttc aattttatcc ttaatttctt ttgcaacagc ctgtttatct
     7621 ccctcgggga gaagtgtaaa cattattgcg gttgccgttc catcctctga aacaacagct
     7681 cccctataca tttctttaga aaaaacatag tttttcaggc tgtctaattg cgattgctca
     7741 acgggcaaat catattcgtc caccagcttg ccaatttcaa taccccattc agaacttttg
     7801 atgtcgagaa tattggttaa acttgtaacg gttgaaacgc cattggtata ttttatgcta
     7861 tcggtaattt gttttatatg ctgaatgact tctgctttaa aaacatcatc ggtttccaga
     7921 acaatcattc ccatatcatt tccgccaaac tgtgttccta tctccttgta caaccgggca
     7981 acagggtcat catcgggcaa agagcttatt atatcagcgt ttatatgcaa gtcttttgcc
     8041 tgataactaa agaatacggt taaacctaca acggctataa ttataagcca tttaaatttc
     8101 actatgccat ctgcgaatct gttcatattt tgttgtttta aaattaatta tacttaaatc
     8161 gttttatagt cagttttagt gcaaaaaaaa ttatcgagat aaaccttttg tgagtattcc
     8221 aattaaacct tcaaaatgag gagcgtattt cacatatttg ttctgtagaa aaaagggtat
     8281 ctcaagccct tttataacca taatgaatac gtctaacaaa acatcaatat ctccaataat
     8341 agcaaattca cccctctcaa caccttgaag gattaacttt ttcaggtttt ctttttccca
     8401 tgcatctaat tcagtccgta aatcatcaat gaaatgaaaa tgttcaaaaa aatcagcttt
     8461 tagcgtttca tgataatttg cagcagaatt tagaatctcc attcttttaa cgagatattt
     8521 ttttaccttt tccgtagaag tcaaatcagg attattaaca ataaaagaca gctgattttt
     8581 caggtttatc atttcaattg atactacctc cctaaataaa tcctccttgc ttgcaaaatg
     8641 ataatataag gaacctttgg ctttcctcgc aattttagcg atttcatcca ttgaagtttt
     8701 atggaaccca aagcgactga atagcttatt cgcaacgctt aaaattgatt ctttggtatt
     8761 atcaatgctc atatcaaatt tgtactaaaa ttgttttacg gtacaaagat tgatatattt
     8821 ttctataatc caatcttttt tttgcataaa ttaacgaatg ttttatcgta gcagtagtgt
     8881 ggtgggaaga ttgcactctt ttgcaaattt tggtttacgt gcggctggtg cgatttgcaa
     8941 atgtgtgcaa tgtgggttgg ggtttcattt cattttcttt tgagcgtcgg aaaaaaacaa
     9001 aataaattcc atcaaattag cactgtcctg tcaaaaagct aaatcctttc ttatttcaat
     9061 tttgtcgtta tcgtaactag ctgtcgggtt gtcttttttt acacttgttg ccaacggtcg
     9121 gcggtatgaa atcgttgggg attgcgggct actttcctgt ccagttacac cgaacttgat
     9181 gcggggcaga atgctcgaat aaccacttaa accccaatgt tttataccgc ttgttagcaa
     9241 ctggcggttc ttgtctttca tgacacaaag ataatactat ttccagttcc actgtcaact
     9301 gtcaatgagt ttttgtcagg gttggaatgt gtgccggcgt ttaaattttt acagaaggga
     9361 agggattttt tctttttatt ttttatactg ttggtagtgc atggaaaatg tcaagctcgt
     9421 gctgcaaact ttggccttgt cagattggtg tggtttgcag caggtgcttg acattatgcg
     9481 gttgggtata aacactttct gtagaaccga aaatattcgt tgtaaacttc ttaccgaaaa
     9541 cattcagaat agaatttgca atcttatttc ccgatcattc tcttagctta tcaaatattt
     9601 taataccttt ttgagttgca atggctctaa tatggtaagc aataatctca tgaataaatg
     9661 ttttattggt aagcatagac ctatcaataa aagcagattc atgaaagcca aatatatggt
     9721 gaagaaataa acgtgataca ttttcaggat ttaagtcttt tctataaacg ccatcgtaaa
     9781 ttcccttatt aatattctct ttgcatttta tatataaatt atttgagata gatgtcttta
     9841 aattcctgta aattgcagga taatatttac tcaggtcgta taataaaaca ggattaaatt
     9901 ctttcgttaa cttaaatttt gtgttgaata gaagaattat ttcttcaata ggacttctat
     9961 tttctttaaa gatggaatct attgatgatt tgttgatatc ttcttctaaa tctgaaaata
    10021 cagttaaaac aagatcattt ttagtttcgt aaaactgata aattgtcttt tttgaaacac
    10081 caagttcaaa agcaatatca tcaatagtta ttctttttat accatatttt ttgaataagt
    10141 taaacgtact atttttaata tttgtttttt tatcagtcac agaatataat gtaaaaaagc
    10201 caactaaatc atttcttcaa tattccaaac aaatgctctt aatccttgtt cttcaacttc
    10261 ttcatttaaa tcttttaatc tttctagaat taatgaaact ttttcatcat taacaatggt
    10321 tatatgagca ttatttagtt ccggccatgt atgcgttcca tcatgttgtt caccttgtga
    10381 agttccacgt cctgtaattc ctgtccattt gctgtaaccg cgaatggcaa gtttgtctaa
    10441 aatcaattga acttcttcag taattgattg attatatgtt attaacactg ctttcattta
    10501 gttatttttt ttaaagattt tattgcctct tttctttgtc gttttatttt accaacacca
    10561 aatatagtgt atattgtagg tattaatatt agtgttacta ttgtagaaaa agtaagtcca
    10621 ctgaaaattg aaattgccat tggtcgccac atttcagagc cggttccttt tgcaacaatc
    10681 ataggtatca tggctaaaat catagtcaac gatgtcataa caacaggtct taaacgagaa
    10741 cgacctgcct ttacaaccgc attttttaaa gaatcacctc tgtcaaccaa caagttggta
    10801 taatcaacta atataatacc atttttaact actatcccaa ttaacatgac taccccaatt
    10861 aatgaaatta cattaatagt tgaattaaaa ataaacagac ctaaaaatac accagtaaag
    10921 gcaaacggta cagaaaacat tatgataaac ggttcggtta atgattcaaa ttgagaagcc
    10981 attacaatgt atacaagtat taatgacaaa acaattaaca acgctaaatc cctaaaagag
    11041 tctttcatat tctctgcact accacccatt tctattgtaa catctctggg tatatccatt
    11101 tttttgattt cagcttcaat atttgcttgc aaagttccta catcaattcc tgacagggca
    11161 gatgaaacct taaccattct gactttattt tcgcgttcaa tattgggcgg agaataaaat
    11221 ctttttaatg ttgtgatttc gctaagttta atcattttac ccataggtgt cataatagtg
    11281 atattagcaa tatcttcggt tgattctcga aattttttat catatctcac tataacatcg
    11341 tactcatttc cttcttcacg atattttgta gcagttaccc cattaatacg gtttcttata
    11401 gccattgcta cctgtgcagt tgtcaatccg aaagatgtca tcttagcatt atccaaaacc
    11461 aattgtaatt cgggcttttc tctatcgcga ctaattaata tatcttttgt tccattaatg
    11521 ttttttagag tattataaat tttttctgct acgatatttg tctcgtcaaa attgtttccg
    11581 taaattttaa catctaagtt gtttccgcct cccattccca tagcgcttga tgctcttgaa
    11641 ctgccggggt caacataaaa cttctcaact tccggtaata cttctatgtc tttacgcatt
    11701 tcatcagcaa tttgaaatat atttctattt ctttctttca aaggtttcat tttgaaagtg
    11761 taattcacaa tataattccc gttttcagta aacaatgcaa ttaaactgtt tccatcacct
    11821 gcaccagcac ttgttgatac tacttcaatt tcagaatatt ttgctctaaa taatgaatca
    11881 agataatgag ctgtttttac tgtttcttct agttttgtac cctgtgctaa ttttacttgt
    11941 gctgaaattc tgtcgttatc agaagccggc ataaattcgg aaccaattag tttaaccaaa
    12001 aaaagtgaaa ccacaaacac aacagttgca atacctataa caacccaacg atgaccaaca
    12061 gcccattcta aagtttttga ataaaaatta tcaattcgct ccaaaatgtt ttgagaaaac
    12121 caataaattt tacctctaaa tgtattttta ttgggcaact ctttatctaa cagttttgaa
    12181 cttaacattg gaaccaaagt catggaaact ataaccgatg ttgcaacgga aattgtaacc
    12241 acccaaccaa gtggtttaaa aagaatacct gttataccac ctaacatggt taaaggaaga
    12301 aacaccgcta taatcgtgag cgtggaagca ataaccgcca aactcacttc acttgtacca
    12361 tagattgcag attcgcggga aaaacctcca cgttcaagtt ttttggtaat attttcaaga
    12421 acaacgatag agtcatctac aactaaacca atagctattg atagtgaaga aagtgtaatt
    12481 atattaattg ttccaccgga aagatacata taaattatac ctgcaattaa tgaaatcgga
    12541 atagagagag aaataataaa agtaggacgg cttctgccta aaaacattat tactactaaa
    12601 acaacaaata caagagcaaa tattaatgtt tctgcaagat tgtttatgga atttatagtg
    12661 ttcacggaag aatccataag tacttctact ttaacatccg atggcaatgt tttatcaatt
    12721 tctgccattt ttttctttat tttatcggca acagtaacag tatttgcgtc tgattgcttt
    12781 tgaatcatga tgcgcacatt tttttcaccg tttgcacgtt cataagattt tacatccttc
    12841 aaactgtcgc agacatttgc aatatccttt aagtaaacgg ttttcccgtt tatgttgctc
    12901 actatgatat ttttaataat atcgctactt tcaaattcgc cttgcaagcg taggggtaag
    12961 tcagattttc ccatttccaa attaccggaa ggaagattta ggttatttgc acccaaaata
    13021 ccggctattt gttctactgt taaattataa gcgtctaatt ttcttggatc aacatccacc
    13081 ataacagctc ttacaggtgc acccatttgt atgatgttgc ctataccatc aattctgttt
    13141 aattgttgaa caattttttt atcaacaata tctttaattc ctgcgtaact ttcattagat
    13201 gtaacactat acatcagaac aggaaccata cttgtactga ttttaaagat tgtaggcgtt
    13261 tcaacttcgt ccggtaaatt tcgttcagcc atcccaactg cattacgtac ttcgttggtt
    13321 gcttcgtcta agtttgtgcc ccattcaaat tctatggtaa ttatcgaaat attatctttg
    13381 gaatttgaac tgattttttt taatccggta agtgacccaa aaccatcttc taattttcgt
    13441 gtaatatttt gttccacatc ggcagcattt gctccctgat aaaatgtaaa tacggaaatt
    13501 atcggcgggt ctattttcgg aaaataatct accggtaaat taatatagga aaacaatccg
    13561 aaaatgacta ctcctataaa aatcattatt gtactaactg gtttattaac tgatgtgcta
    13621 tatatgctca tattttttcc cttttaattg tagaaaaatg tgtttaatta tttgatgaca
    13681 gaaataggag tttgattttc gagtttattc tgaccaacta caataatttc atcaccttcg
    13741 tttactcctt ccaaaatctc tattttgtcg tcaaacattt taccggtttt aacaagtgtt
    13801 tttattgcaa cattgttttt atttacaaat aaataaatgt cgtttgtgcc ggtttgtttt
    13861 attaatgcaa tactgggaac taaaatagca cttccttttc caaggttcag ttggatttta
    13921 gcaaacatgc cgggtcgtaa ttttaaatta gcgttttgta taacaacttc gagaatgaaa
    13981 gtttttgtaa cattatcaac agtcggataa atttttatta tttgaccgtc aaatttcata
    14041 tcgggatata gttctgattt aatatttgct ttcatcccga catttatcaa tggaaaataa
    14101 tttgatgaaa ttccgaccaa ggctttcaat tggtttattt ggacaatgga aataattgcg
    14161 ggttttccaa ctgaagaaac tggtgtacct gaataaattt caccatcttc aaaatatttt
    14221 cctgatacta cacctgaaaa aggggctttt aattgtgtat tatctaataa aaactggtaa
    14281 gagtttttag ctatttcata agccgatttt atttggtcgt actgctggtc ggcaatgctt
    14341 ttagtttttt ttaaggtatc taaccgcttg taatcagttt ctaatttcat catattaagg
    14401 cgcgctgttt caaggtttgt tctgtccatt aaagccacta tttgaccttt tgtaacatta
    14461 tcgctaattt caacattaat tttctctatc cttccgggtg atgccggagc caaatgcact
    14521 tcttcaaatg gaattaaagt agcagtataa tcaatgtttt gattaacttg ttcttgtttc
    14581 agtaccattg attttaccga aactaatttt aaactatctt ggttttcggt ttttgtttta
    14641 tcagattttg aggtacagga agaaaaaact actattattc ctgaaattat acaacccgat
    14701 aaaagtttta atgcgttcat gattatgtta tttagaagtt actatttatt tttcttaatg
    14761 ataattcggc gtttagtaat tgaagtaaaa ttcctgcata tgccgattcc gcgtttaaat
    14821 attcattatt tgcacttgtt aattcaagac ttgagacaat gccttgctcg tattttagat
    14881 tcattttatc aaatacttct tttgctattt ctacatttgc cttttgattt tgaaactgtt
    14941 cataaagatt tttgtaatta tacctaagtt gcttttcctg taaagtaagt tgatcggtta
    15001 gtaattcctt attattcaga gaaatataat agtctgtttt tgcctggtta acctgatatt
    15061 ttctaaaccc actagaaaaa atcggaatat ttaatgacaa acctactact tggttaggag
    15121 acaagtcaaa tttgggtttt attagttttt ctgtgtatga ataatatgca gctaatgttg
    15181 gtaagtaagc agccctctta agtttaatat ttttttgtgc aatatctccc tgcattgaaa
    15241 ttaacttata atccatatta ttttctatat taaaagtatc atccattaaa gattgggtta
    15301 caaacttttg ttcaagcatc tctaaatcag tagtaagttt tatttcttgt tcagaactta
    15361 aacctaactg taagcgcaac agattgtaac ccatttcaac ctgacgttca gttgctttaa
    15421 ttgcattgtc gatggaagta attattatta aaagtttttt tggttctgta agttcaatta
    15481 ttccagtatt agccaaatta tttgtttttt cgtaaataag ttgaatgtta tttttgtttt
    15541 ctaatctgat tttcagcaat ttttcactga ctaatatcat gtaatatgcc tgaatagttt
    15601 gttcttttac attcaattca tctttttgat aactttgttc tgtaatcgtt tttgcgagtt
    15661 ttgaaagttg aataccaaca tagtaactac cactaaaaac taattgactt acagacaatt
    15721 tagcattgct tgtcgggttg aattctatta ctgcaggagg cgccatttca ctaatcttaa
    15781 attctgcttc tgctcctaaa aagttattgt agtcaactga agcgttaatt tgtggcaagc
    15841 cttgagatat agtttcttta attttctgag acgacttatc tatggtaagc ttcgaactta
    15901 tcaatatttt attatgctct atggcataaa caatgctact gtcaagcgat agtgtcaagg
    15961 tttcttgtgc ttttaataca ttgctacata aaattaatat gcaacaaata gtaattttta
    16021 aataatcatt catgttctgt tctgctttta tgtatttgaa atatttttag ttgcattatg
    16081 catattattg cattatgcaa caatgtcatt ttagacttca taatttgcta cccttacttt
    16141 tatatggtca attacttttg aaaacacctt taaatctgtt tcattaatac ctttctgtaa
    16201 atcatcaaca atttcatcca ttattttaag gtaaacttca atagtcgtct gtcctgtttt
    16261 tgttatcatt aaatagtttt ttcttctgtc attcttatct tctgcttttc ttaccaatcc
    16321 tttttcttct aaagaatctg taatccgtaa tatggttgat ttattcttcc ctgtatatac
    16381 agcaagttgt ttttgaatta catcaaaatc ttttatatgc ataagataca gtaaacctaa
    16441 ttgctctact gtcagatgaa tgttagtttt ttctttcgca atatgtaaca acactctaaa
    16501 gaattccttt ttcagttttc ctatttcacg gaaatcaata tatctttttt ccatatttct
    16561 tattttcttg gtcgcaaaat taataaataa ttggaatatg caactaaaat tgaaaatatt
    16621 ttttcgtaaa ataaatttca gcgcaagaat gggcgtttgt agcttttttg caagccattg
    16681 aattgtaaat tggtatgtgg gggcttgcaa atgtgctgca atgaagaata gccaaaacgg
    16741 ctttgccgaa aaagtgcggt ggggtaaaaa tttaaacttt ggggtcggac tgagtgtcgg
    16801 cttattttat tttagtcagt acttgtcaag ttgcagtgaa atatcagatg tgtaattgtc
    16861 tttattccgc ttgttgctaa cgttcccacg cttggcattg tgcaaattac gtaaaacttt
    16921 tcaatccacc aaaacatttt cattgcataa tgctaagcgt gtgttagcgt ggcgtgcgta
    16981 tttgtcagcc cggatgtcgc gcggtcgcac gtgtgtgtcg gctgacagat cacacattgc
    17041 gggatggctg tcgagcgatc ggctgtcagg gtgtggtgtt gacctgctgt catacgtttt
    17101 atttacggtt gtcattatat ttttagtcag tcacacgatg ttgctccttt tctctgtcct
    17161 cgtgtaagtc aacagcgaca ttttaaacgg ccacctattt catgccggcg acgtgtcggc
    17221 caaaatgcca atggatcgtt tttcagccgg caaacatcac cgtaatcaat tctcagccgt
    17281 tttgcaccaa ccgtaaattc tcaaagaacg tgtcttactt tttttgtcca gggcaacttc
    17341 tcaatccaca ccggtcaagt catttttacg catgcacgct aacggtcggc ggtatgcgcc
    17401 gtaggggaaa tcggagccac tttcctatca cccgttactg acctcgatgc gagctgaaac
    17461 gcttgatata ccactgaaac ccctatggcg tatgaccgct tgttagcggc tgtatttttt
    17521 tattagagtt caattgtccg ttctatgttt atttgttcca aaaatgtttc atcgtgcgac
    17581 acaacaatca atgttccttg atattcattt atggcagtcg tcaaaatctc aacattctga
    17641 atgtccaagt tgtttgtcgg ttcgtcaaag ataataatat caggcgactt gctgtttatt
    17701 gtcaagcaac aaagcaataa acgcattctt tcaccaccac tcaacgcact acaagatttg
    17761 tcccaatctt cttttgtaaa caaaaagcga tttagtcgga ttttgatttc gtgttcttgt
    17821 aatgcagaaa cattgaattg ttgggcttgt tcgtaaactt tcagtttgtt gtcgagcaaa
    17881 gaatagtctt ggtcaatgta aaccgatttg ttgtttgctc ggtaaattgt tcctgtttgt
    17941 ggctcgatgt cgcccaaaat gagttttatc aaagttgttt tgcccgagcc attttgtcct
    18001 ttcaatgcaa tgcgttcgcc acttgtaatt tggaaattca agttttctgt ccaaagcggt
    18061 tgcatgttgt aagcgaaatt tatgttcgtc gctgtgaaca aaatttttcc tttgtgtaac
    18121 gctgaattat caaaaccgaa tttcattttg tcaatgtcgg gcaacgaaga acgaagttct
    18181 tgtaagtctt gagaaatacc accaattttt tctgcgtgag tacttttgag ttttgatgtg
    18241 ctgttttcgg cattgtttcg caaagtgttc atcattattc gggcaacacc tgctttttct
    18301 tgttttcctt ttccacgatt gtccaacttt tgttgtcgct ccaaagtttc ccgctctttt
    18361 tctttggctt ttcgcaatgc cttttccttg ctttgaatgt cttggctcaa agcattgttt
    18421 tcaatttgct tttgttcttt gtaaaaatcg taattaccgc cataaacttt aatttcgtgt
    18481 ttactcaatt cgcaaacttg gtctaaaaga ttgagtaatt ttctgtcgtg gcttacaaca
    18541 atcaaagtgc ttttagtggt ttgaataaag ccatacaaaa gttttcggct tgcaatgtct
    18601 aaatgattac ttggctcgtc caataaaatc aattcgggtt gatgaatgga aatacctgcc
    18661 aaaaaaactt ttgttttttg cccaccactc aatgcttcca ttttttgcga caagtctata
    18721 tcaattaatt gccaatgttg taaagcgtca ttgcaacggt cttcaattgt ccaatcgtca
    18781 ttaagcaaat tgaaattttc ttcggttgtg tttccgttca aaatttcttt cagagcgttt
    18841 aatttcttgt caattttcaa cgcttctgca atggtcaaat gattgaattg tccgaaaatt
    18901 tgcggaataa aatatggctc ggtttcaacg attatttgtc cgtttgacgg ttgtaattct
    18961 ttggcaataa ttttaagcaa agttgatttt cctgctccgt tgttgccgat taacgctgtc
    19021 ttttcgtgat tgtttactgt cagattaatg tcgctaaaca gtaaatcctt gcttgggtgt
    19081 gtatatgaaa tgttttgtag aataagcata atttctttcg ttaaagatga aacaatacga
    19141 caaccgcttt gtggttgctg ttctgatttg tctaaaagaa attatttttc acatttcgtc
    19201 ttacttaaat ttttgtgtct gcaaatatac gaattttctg tttagcactg tcggtcgtaa
    19261 tatagccgct aacgctccag tgtatttgct gtaagggatt gcggggggaa taattacatt
    19321 atc
//
LOCUS       DAOTCW010000038        19162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1559_length_19162_cov_38.046632, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000038 DAOTCW010000000
VERSION     DAOTCW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 19162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19162
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1559_length_19162_cov_38.046632"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..469)
                     /locus_tag="P5335_04805"
     CDS             complement(35..469)
                     /locus_tag="P5335_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74232.1"
                     /translation="MERTLKIILFLLFFFVSQNVFCQNEKALCKAVSNFDFKKVESIV
                     KKVIKTNKKGQIYFNGEGSGYQINLSPCFDSITNWLKKQDCVEDAYWDKCQIKQAIYP
                     GHSTIGVKFRTKNGIIEKCFPRSPNSPLFLAITCFILIFEKE"
     gene            complement(542..1033)
                     /locus_tag="P5335_04810"
     CDS             complement(542..1033)
                     /locus_tag="P5335_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74233.1"
                     /translation="MKNLILFIVLLIFNSCSTDSNKSNDAVNIDNGVTIFLKSIEGNN
                     LLNTTNYNSNAFKIYYKINNENIEINNPNNDFPRNYYINNESNPISMKLFLNHSVTEE
                     YPITLIEWNTNDTDTIKTYFRRGIENNTDYEICEKIWLNDVLIWDISTANGLTEREIT
                     ILK"
     gene            complement(1041..2237)
                     /locus_tag="P5335_04815"
     CDS             complement(1041..2237)
                     /locus_tag="P5335_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HRZ74234.1"
                     /translation="MNKNRIFKNLKICFVLASLTLLFSCENDNIQNSSKHQNNIAEID
                     AKELALSLSKGSNSVSRGIDITKTIMDATTYKTESNENAFYAINYNEGGFVVISADNR
                     ISPILAYSDDGKFSNNTEEIPDAVISWMEEEKEAIEYVKEMELTQMPEIKLEWDSVQN
                     LLPAPTGGNNCNDTFYQKDPLVNTIWAQGATYNNLISLNCSNSSGGKAPTGCVATAMA
                     QVMKYHQKPVNYNWSNMPYNYGTSDTQLLMKNIGLAVGMDYGCSSSGADTTTDVASAF
                     NNDFNYHGATYTCNYSTSIITQQLNLNKPVILRGGRKRNGITWPWNYYTGGHAWVCDG
                     YQSMMIYFRDDAGNCTGGGNGYLFLHMNWGWGGYCNGWYNAHNFNPASSTFNYKRGIV
                     YNITPN"
     gene            2813..4297
                     /locus_tag="P5335_04820"
     CDS             2813..4297
                     /locus_tag="P5335_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRZ74235.1"
                     /translation="MMTKQTETQLEQYFTPFRDHIIGINQVFQSPFGQKKIIYTDWTA
                     SGRLYRPIEEKLINQFGPFVANTHTETTISGTAMTMAYHQARQIIKSHVNAANTDVLI
                     TDGTGMTGVVNKFQRILGLRIPENLKEFTTIPEALKPIVFISHMEHHSNQTSWLETIA
                     DVEVIPANEQGLFCIDNLKTLLEKYKDRVFKIASTTSCSNVTGIKTPYHEVAKLMHQN
                     NGVCFVDFACSGPYVQINMHPEDSESYLDAIFFSPHKFLGGPGTCGVLIFNQNLYKNN
                     VPDCPGGGTVSWTNPWGEHKYIDNIEDREDGGTPGFLQVIKTALAIQLKEQMGVNNIL
                     NREHEIVELVFEQLGNMENINILAGDHQDRLGVISFYIDALHYNLGVKMLNDRFGIQT
                     RGGCSCAGTYGHYLLHVDQETSNDLVCQIASGDLIKKPGWIRMSIHPTTTTEEVLYVC
                     ESIKQVAKHHKTWSKDYLYDAKTNNFRHKDDSLPEKEMVKNWFL"
     gene            complement(4316..5185)
                     /locus_tag="P5335_04825"
     CDS             complement(4316..5185)
                     /locus_tag="P5335_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HRZ74236.1"
                     /translation="MQLVVFILVYPILWLISILPYRLFYLFSDFICFFVYRVIGYRKE
                     TVRENLLLAFPEKTTEERLAIERKFYQHMCDLFLEMIKTLTITEDEMNKRFTISNLEV
                     LQEMESKGKSIALMTGHYASYEWSMSVNKKLSVKGYAIYKKIANKYFDKLVRDIREKF
                     GATLITTKETIPTIKANYIAGVQSIYGFASDQSPKASRAINWGPFFGIETPLQTGAEM
                     LSKKFDMNVIFMRIRKVKRGYYDCSFEVMTYDAKSIPDFELTHQFMRKVEEQILEAPE
                     YYLWTHKRWKHRK"
     gene            5290..5931
                     /locus_tag="P5335_04830"
     CDS             5290..5931
                     /locus_tag="P5335_04830"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRZ74237.1"
                     /translation="MNIALIVLIAANVLISFKAFEDQFFFRRYEFHIGSIRAGEHIRM
                     FSSAFLHVDIAHLAFNMITLYFFAPVVSSYLGIPSFLVVYFGSLLCGSLLTLQFHKNE
                     YNYRAVGASGAVTGILYAAILLRPDMDLYFFFIPLPIPAYIFGIGYLLYSIYGMKSRN
                     DNIGHTAHFGGAIGGYVITLIKEPQMLTENTLMVGLLAIPIIILFIMAKMGKI"
     gene            5984..6673
                     /locus_tag="P5335_04835"
     CDS             5984..6673
                     /locus_tag="P5335_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496643.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRZ74238.1"
                     /translation="MKKSIIILFAVAMTTMNAQENKLVPQISVSGEGKMKVTPDRVVV
                     NFGVDNVGKDATEVKKMNDETVDKVIKFIKKFGIPTADFQTTNVSLHKTYDYDKKKHS
                     YQASQSITITLKDITKYDELMMGLVDTGINNISNVEFKSSKMEELKTEARKLAILDAK
                     KKAEDYVSVLNQKVGKAILITDNSQPYYPPMYKNMAYAEMATDAGLDRETLAIGELEI
                     ITNVSVSFVLE"
     gene            complement(6725..>7303)
                     /locus_tag="P5335_04840"
     CDS             complement(6725..>7303)
                     /locus_tag="P5335_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRZ74239.1"
                     /translation="LKANGVKATILDHDSDRVDLLRKMGFKVYYGDATRLELLKAVGA
                     ENAKIFIAAIDNPEVNLSVVELLRKHFPNLNILTRARNRVDAYELIDHGVDKIYRETL
                     YTAVHLGVDALTQLGHRKYSATRQGQRFIKYDEAAIRKLAAKRHDKMAYLATVKDELE
                     MQEQLLKNDLHINFSATDHAWDSEHLKKQITE"
     assembly_gap    7304..7403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7404..8751)
                     /locus_tag="P5335_04845"
     CDS             complement(<7404..8751)
                     /locus_tag="P5335_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HRZ74240.1"
                     /translation="MDKGFFIQAIVYLTSAVVCVPIAKKLGLSSILGYLFAGIVIGPF
                     VLGFIGSEGEDIMHFAEFGVVMMLFLIGLELDPYKFWKMRKFILGMGSMQLIGTGVSI
                     FILCQLFLDWNWQTTVVIALALSLSSTAIVLQTLKEKGLSQTSMGRASFSVLLFQDIA
                     VIPILAIIPLMMSGQTIPDDDLKSSLIIDLPGWIQTLIVLAAIIAVYFAGKYIVVPLL
                     HIVAKTRLQELFTASALLLVMAVSYLMQLVGLSPALGAFMAGVVLANSEFRHELEGDI
                     APFKGLLLGLFFIGVGASINFKLIIDDPAYILIFGAILTSIKFFVLFGIGKFYKKSND
                     QNLLFSFGLSQAGEFGFVILSFSMQLNILPSILANQMMAIIALSMISTPFLLLINEKW
                     IDPFFGVKEKVKKPHDIIDENNEVIIAGFGHFGSTIGRLLKANGVKATILDHDSDRV"
     gene            complement(8744..9358)
                     /locus_tag="P5335_04850"
     CDS             complement(8744..9358)
                     /locus_tag="P5335_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRZ74241.1"
                     /translation="MAQNKILILFAHPLFEKSFVNEALVRHIPKSKNITFHDLYEEYP
                     EFDIDVKREQALLQKHDIIIWHHPLYWYSCPPLLKQWIDMVLEYDWAYGKNGIALQNK
                     LIFQAITTGGKKENYCEAGRDRYSIPELLEPFNQTAKVCHMHYLPPFVIHGTHQMNES
                     EADNYGKIYADLLLYLKSNELDIKEMNTHFYLNEWFNSKSKKHG"
     gene            complement(9415..10761)
                     /gene="purB"
                     /locus_tag="P5335_04855"
     CDS             complement(9415..10761)
                     /gene="purB"
                     /locus_tag="P5335_04855"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRZ74242.1"
                     /translation="MNALNELNAISPIDGRYRNKTVSLAAYFSEEALIRYRVLVEIEY
                     FIALYELPLPQLSTVNPSVFEKLRDLYRNFSTQDALAIKEIEKTTNHDVKAVEYFIKN
                     GFDALGLESYKEFIHFGLTSQDINNTAIPLSTKESFEKVYLPHLISVISKLKDMSQEW
                     KDVPMLARTHGQPASPTRLGKELGVFVERLEEQMRQLFNIPFAAKFGGATGNYNAHHV
                     AYPQINWKKFGTHFVEETLGLHHSFPTTQIEHYDHIAAYFDCLKRINTILIDLDRDIW
                     TYVSMDYFKQKIKAGEIGSSAMPHKVNPIDFENSEGNLGMANAIFEHLSAKLPLSRLQ
                     RDLTDSTVLRNIGVPIGHTLIGFEATLKGLNKLLLNRSKLEEDLERNWAVVAEAIQTI
                     LRREGYPNPYEALKGLTRTNEVMNQKTIHEFIHSLAVSETIKAELLQITPSNYLGI"
     gene            complement(10852..11538)
                     /locus_tag="P5335_04860"
     CDS             complement(10852..11538)
                     /locus_tag="P5335_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRZ74243.1"
                     /translation="MKKLVVLTGAGISAESGIKTFRDADGLWEGHDIMEVASPIGWKK
                     NPELVLAFYNKRRKQLLEVNPNQGHFILAELASHFDVHIITQNVDNLHEKAGSKNVLH
                     LHGELLKVRSTKDEKYILDWQDDLLSNHTDPNGFPLRPHIVWFGEDVPAIEEAIDLAE
                     QADFFVVVGTSLQVYPAAGIMHYTKPETPVFYIDPNPASVLELANPLEIIPMNGSEGM
                     AVLKQRLLDL"
     gene            complement(11550..12344)
                     /locus_tag="P5335_04865"
     CDS             complement(11550..12344)
                     /locus_tag="P5335_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRZ74244.1"
                     /translation="MSTQEYIESGILELYVYGKLNELETEEVHKMAQQHSEIREEIVA
                     IEKTVILLSGSVAPHLSGQHYEAIKQQIFENKPNVVEIKKEPKKSNISQYLGWAAAIL
                     LLLGGAYLYQQLDITKKDVLSLKSDKKKMKEEYTVLVSKNKVNEDMLSFVRDTKNTII
                     ALGGQEVSPEAYAKVYWNQETHTVYVDAAGLPEPPEGMVYQVWALKLNPLTPLSIGLL
                     DNFKGKSMLIFPVDNVIEAEAFGITLEPDGGSTTPTLENLYTLGKV"
     gene            complement(12341..12874)
                     /locus_tag="P5335_04870"
     CDS             complement(12341..12874)
                     /locus_tag="P5335_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRZ74245.1"
                     /translation="MTQEELLPLIYKKDDKAFTLLYDMYAKSLFGIIATLLKNPEDAQ
                     DALKEAFIKIWKNIDTYDENKGRLYSWMVTITRNCALDKLRSKGFSTSLKNISADNFV
                     HLLDDQTRMNGKIDTIGVQEFVKKLKPRCIKLIDLLFFKGFTQQEASDELEIPLGTVK
                     THNRNCISELRNLLHVS"
     gene            12930..>13582
                     /locus_tag="P5335_04875"
     CDS             12930..>13582
                     /locus_tag="P5335_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRZ74246.1"
                     /translation="MNEMLKTIEYLTFLEGFLTENRKSKFLEILQLRTQHFTVAIEDV
                     FQMHNTSAVMRSCEVFGVQEVHVVEQKYGKKIDTEIAMGAQKWVDIKQYPSIQNCISG
                     LREKGYQIIATTPHTDSCLLEDFDISKKAAFFFGTEKDGLSEEVMQQADGFLKIPMVG
                     FTESLNISVAASIILQSVTQRLRNSSINWQLSEEEIFLKRIDWAKKTIKDCDRVEER"
     assembly_gap    13583..13682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13809..14576)
                     /locus_tag="P5335_04880"
     CDS             complement(13809..14576)
                     /locus_tag="P5335_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRZ74247.1"
                     /translation="MRYFVVFLLFLSLSSVTAQIAERTQRVSGTVLDDTKSKPLPNVN
                     IINLNSVRGTITDDKGVFEINVQVNDTLHISLIGFQSIRVRVTNDWIKSKITQISLTE
                     KAIALEEVVLTPYNLTGYLQVDAKLIPVKENYRYGISGLPYGYEAGEYSPNAFNRVLG
                     SIFNPTDMLYNFFGNKPKELRKLKEMNKDDTVRNLLASKFDRETIAIMLGVNKKDIAE
                     ILERCNYSEQFIKTANDLQVLDAINDCYEEYKILKRK"
     gene            complement(14664..16556)
                     /locus_tag="P5335_04885"
     CDS             complement(14664..16556)
                     /locus_tag="P5335_04885"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRZ74248.1"
                     /translation="MNKFEQLGLSESLQRAIIDLGFENPTEVQEKAIPLLLEQETDLV
                     ALAQTGTGKTAAFGFPLIQKIDANNRNTQALILSPTRELCLQITNEIKLYSKYEKGIN
                     VVAVYGGASISEQAREVKRGAQIIVATPGRMQDMINRGLVNITQINYCILDEADEMLN
                     MGFYEDIVSILSTTPDEKNTWLFSATMPAEVSRIAKKFMHEPVEITVGTKNSGSATVS
                     HEFYLINARDRYEALKRLADANPDIFSVVFCRTKRDTQAVAEKLIEDGYNAAALHGDL
                     SQAQRDSVMKSFRGRQIQMLVATDVAARGIDVDNITHVVNYQLPDEIETYNHRSGRTG
                     RAGKLGTSIVIVTKSELRKISAIERIIKQKFEEKTIPSGIEICEIQLLHLATKIKDTE
                     VDHEIDNYLPAINEVLNGLSKEELIKKMVSVEFNRFINYYKKNRDISNQSSGSERRER
                     DDREPRENNSGATRFFVNIGSRDNFDWMQLKDFLRDTLELGREDVYKVDVKEGFSFFN
                     TDAEHADKVMDVLNGMLLEGRKINVEISKNDGGGRRDHNGRSSGGGGGFRGERNSGPR
                     SGGFGSRTERSAAPRGDRKEGGFRPRTEGSSSERPARRSEGGERNERPASRSFEAAKT
                     RRPRRS"
     gene            complement(16707..17291)
                     /locus_tag="P5335_04890"
     CDS             complement(16707..17291)
                     /locus_tag="P5335_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HRZ74249.1"
                     /translation="MPLVFASNNPNKIKEIQLLLPNTLQILSLEDIGCLEDIPETAPT
                     IEGNAIQKANYVTEKYGYPCFADDTGLEVAALDNAPGVYSARYAGEQKNADDNMAKLL
                     DNLSDKTNRKAQFKTVICLNLNGNQHLFEGIIKGEIIHEKRGSAGFGYDPIFIPNGYS
                     NTFAEMSMSEKSKISHRGLAVEKLVAFLKQKGIK"
     gene            complement(17304..17879)
                     /locus_tag="P5335_04895"
     CDS             complement(17304..17879)
                     /locus_tag="P5335_04895"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HRZ74250.1"
                     /translation="MKTKALLFGLFLLVAAAQLYVPTEMIWQNQNILDTGTIMKFKCE
                     PVDPYDPFRGKYISLRFANSTIGVKNANRYQYNQTIYATFKQDTTGYAVMDKIYTAAP
                     KERTDYLELTVDYANNYDSIQKISVSYPFDRYYMNEYKAPMAEQTYAESVNDVKKSVY
                     AEVAISNGTGVIKKVMIDNQAIEDYVKKQPN"
     gene            complement(17869..>19162)
                     /locus_tag="P5335_04900"
     CDS             complement(17869..>19162)
                     /locus_tag="P5335_04900"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HRZ74251.1"
                     /translation="TPQHKNSMQKQHLNELLEHGIITPEVATAITNYYQNKQTTASKL
                     PIIFGIFGALLGGLGIILLIGHNWDEFTTTTQVVLAFLPLLLAQIASGYTILKKPESI
                     AWRESSGAFLFCGIGASIALIAQIYHLPGNFNRFVLTWMLLALPLIYLLKSSVVSLLF
                     LIGITVYNMTIGYIDYPNVQNYKYWLLLLAVAPHYYQLIKQKPNGNFTTIHHWFIALT
                     LLINLESLDKNNNELIFITYGFLIALFYLLGKTAYFRHHHTIANSYLTIGKLGGLTLL
                     YMASFRFIWKPLLLKNLAPEPLLLTVTLTLFVGCALLLYQLNKRKAATPFTLFHFNFI
                     ALAVFFITAQVSIIATVILSNLYILSLGLQEIQKGNKTHSIARLNFGILIIAILTGCR
                     FFDENMTFIVRGVMFIIVGFGFFALNYFLLKKRKQHEN"
BASE COUNT         5698 a         3556 c         3522 g         6186 t
ORIGIN      
        1 taactagtaa ctaacatcaa atatagacaa ctttttattc cttttcaaaa attaatatga
       61 aacaggttat tgccaagaag aggggagaat ttggggagcg gggaaaacac ttctcaataa
      121 ttccattttt cgttctgaat ttaactccaa tggtagaatg ccccggatat atcgcttgtt
      181 taatttgaca tttatcccaa tacgcatctt ctacacaatc ttgtttttta agccaatttg
      241 taatagagtc aaaacaagga gataaattta tttgataacc gctaccttcg ccattaaagt
      301 atatttgccc ttttttattc gtcttaatta ctttttttac gatactttct accttcttaa
      361 aatcaaaatt ggaaacagct ttgcaaagtg ctttttcatt ctgacaaaac acgttttgtg
      421 aaacaaagaa gaataacaaa aacagaatta ttttaagtgt tctttccatt gtcatgtcgt
      481 cttttagcgt ttggggagcg ggctttgcaa gcccaacgaa acactagcta ttagctgtag
      541 tttattttaa tattgtaatt tctctttctg ttaagccatt tgccgtgctt atatcccaga
      601 taagaacatc atttaaccaa attttctcac atatttcata atcggtatta ttttctatac
      661 ctcttctaaa atatgtttta attgtatcag tatcgttggt attccattca attagagtaa
      721 ttggatattc ttctgtaacg gaatggttta aaaataattt catactaatc ggattacttt
      781 cattattaat atagtaattt cttggaaaat cattatttgg attatttatt tcaatatttt
      841 cattgttaat tttataataa attttaaagg cattggagtt gtaattagta gtatttaaca
      901 agttattgcc ttctattgat ttcaagaaaa ttgtcacacc attatctatg tttacagcgt
      961 catttgattt attactatca gtactgcaac tattaaatat taacagtact ataaaaagaa
     1021 ttaaattttt catattataa ttaattaggg gttatattgt atacaattcc tcttttatag
     1081 ttgaaagtgc ttgatgcagg attaaaatta tgggcattgt accaaccgtt acaatatccg
     1141 ccccaacccc aattcatatg aagaaataaa taaccatttc caccacctgt acagtttcca
     1201 gcgtcatctc taaaatatat catcattgat tgatatccgt cgcaaaccca tgcatgccct
     1261 ccagtataat aattccatgg ccaagttatt ccatttcttt ttctaccacc tctcaaaata
     1321 actggcttgt tcaaatttaa ttgttgagta ataatacttg tactataatt acaggtatat
     1381 gttgcaccat gataattaaa atcgttatta aatgcacttg caacatcagt tgtggtatct
     1441 gctcctgaac ttgaacaacc ataatccatt cctactgcca gacctatgtt cttcattaat
     1501 aattgagtat cagaagttcc ataattatat ggcatatttg accaattata atttactggt
     1561 ttttgatgat atttcatcac ttgagccatt gcagtagcta cacaaccggt cggtgctttg
     1621 cctccagaac tatttgaaca atttaaagat attaaattgt tataagtagc accttgagcc
     1681 caaatagtat ttactaaggg atctttttgg taaaaggtat cattacaatt attacctcca
     1741 gtaggagctg gtaataaatt ttgaacactg tcccattcaa gttttatttc tggcatttga
     1801 gttaattcca tttcctttac atattctatt gcttcttttt cttcttccat ccaagaaata
     1861 actgcatctg gtatttcttc agtattattt gaaaatttac catcgtcaga atacgctaaa
     1921 attggactaa ttctattatc ggctgaaatt acaacaaatc ctccttcatt ataattaatt
     1981 gcataaaatg cattttcatt gctttctgtt ttatatgttg tagcatccat aatagttttt
     2041 gtaatgtcaa ttcctctgct cacactatta ctccctttag ataaacttaa agctagttct
     2101 tttgcatcaa tttctgcaat gttattttga tgtttggaag agttttgaat gttatcattt
     2161 tcacatgaaa acaaaagtgt taaacttgct aaaacgaaac agatttttaa atttttaaat
     2221 attcgatttt tgttcataat ttttttaagg tttataatta ataaaaattt atcatttcga
     2281 tgctaatttt acattagttt taaccaaaat gtagttaaaa ttgcatatgc tagtaactaa
     2341 cttcaaatat agataacttt ttcatcgttt ttaataatta atatgaaagt gtttaatctg
     2401 cgaagtcatc tgtttggttt gcgaaagcat ctgtttggga tgcgaagcgt tctgtatggt
     2461 tcgcgaagcg ttctgtttgg tatgcgaagg attctgtttg gttcgcgaag tgttctgttt
     2521 ggcacgcgaa ggcatctgtt cggtctgcga agcgttctgt ttggtaagcg aaggcatctc
     2581 tcatgtccgc gaagtcatct gtttggtctg cgaagcgttc tgtttggtac gcgaaagcat
     2641 ctgttcggtc tgcgaagcgt tctgtttggt tggtaatgac cgttatttca tttgaaacat
     2701 catcactatg ccacagacag ctgttttgag taaccataat tagaagttca cccaccaaaa
     2761 cacaccacac aactaaacaa aaaaatgtat atttgtccaa atatttcccg ctatgatgac
     2821 caaacaaacc gaaacgcaac tggaacagta tttcacccca ttccgagacc acatcatcgg
     2881 catcaaccaa gtgtttcaat cgccttttgg ccaaaagaaa atcatctaca ccgactggac
     2941 ggccagcggc aggctgtatc gccccattga agaaaaactc atcaaccaat tcggcccctt
     3001 cgtggccaat acgcataccg agaccaccat ttccggaacg gctatgacga tggcctatca
     3061 tcaagctcgc caaatcatca aaagccatgt gaacgctgcc aacaccgatg tgctcatcac
     3121 cgacggcacc ggaatgaccg gcgtggtgaa taagttccaa cgcatactcg gcctccgaat
     3181 tcctgaaaac cttaaagaat ttaccaccat acccgaggcg ttaaagccca ttgtgttcat
     3241 ttcgcacatg gagcaccatt ccaaccaaac ctcttggctg gaaaccattg ccgatgtgga
     3301 agtaatccct gccaacgagc aaggactttt ttgtattgac aacctcaaaa cattactcga
     3361 aaaatataaa gaccgcgtgt ttaaaatcgc ctccaccacg tcgtgttcaa acgtaacggg
     3421 gattaaaaca ccctatcacg aggtggcgaa actcatgcac caaaacaacg gagtttgctt
     3481 tgttgatttc gcttgttcag ggccttatgt gcaaataaac atgcatcccg aagattcgga
     3541 gagctacctg gatgccattt tcttttcgcc ccataaattc cttggcggac ccggcacttg
     3601 cggcgtgttg atttttaatc aaaacctcta caaaaacaat gtgcccgact gtcccggtgg
     3661 aggaaccgta tcttggacca atccatgggg agagcacaaa tacattgaca acattgaaga
     3721 ccgtgaagac ggcggcacac ccggatttct tcaagtgatt aaaaccgcgt tagccattca
     3781 attgaaagaa caaatggggg tgaacaacat cttgaaccgc gaacacgaaa tcgtagaact
     3841 tgtttttgaa caactgggca acatggagaa catcaacatc cttgccggtg atcaccaaga
     3901 taggctaggg gtaatttcct tttatatcga cgcgttgcat tataacttgg gtgtaaaaat
     3961 gctcaacgat cgctttggga ttcaaacccg tggcggatgc agttgtgcag gaacctacgg
     4021 gcattatcta ttgcacgttg accaagaaac gtcaaacgat ttggtgtgtc aaattgcctc
     4081 aggcgacttg attaaaaaac cgggctggat aagaatgtcg attcatccaa caaccactac
     4141 cgaagaagtg ttatacgttt gtgaaagcat caaacaagtg gccaaacacc acaaaacatg
     4201 gagcaaagac tacctttatg acgccaaaac aaacaacttc cgccataaag acgattcact
     4261 tcctgaaaaa gaaatggtga agaattggtt tttgtaaggg tagaaataga agggtttact
     4321 tgcggtgttt ccaacgtttg tgtgtccaca aataatattc aggagcctca agaatttgct
     4381 cctctacttt acgcataaac tgatgggtta actcaaaatc gggaatgctt ttcgcatcgt
     4441 aggtcattac ttcaaagcta caatcatagt acccgcgttt cacctttcgg attcgcatga
     4501 agattacatt catgtcaaat tttttcgaaa gcatttccgc accggtttga agcggtgttt
     4561 caataccaaa aaagggtccc cagttaatcg ctcgagaagc ctttggggat tgatcacttg
     4621 caaaaccata gatgctttgc acgcccgcaa tataattggc tttaatcgtt ggaatggttt
     4681 ctttggtagt aattaaggta gctccaaatt tttcgcgaat atcccgcact aatttatcga
     4741 agtatttgtt cgcaatcttt ttataaatgg catacccctt aacggatagc tttttattca
     4801 cggacatcga ccattcataa ctggcataat ggccggtcat caaggcaatg ctctttcctt
     4861 tgctttccat ttcctgcaac acttcgaggt ttgaaatagt aaagcgttta ttcatttcat
     4921 cttcagtaat ggttaacgtt tttatcattt ctaaaaacaa atcacacata tgctgataaa
     4981 actttcgttc aatggcaagt ctttcttccg tggttttttc cggaaaggcg agtaacagat
     5041 tctcccgaac ggtttctttt cgataaccga taacgcgata tacaaaaaaa cagataaaat
     5101 cagaaaaaag ataaaacaac cgataaggaa gaatggaaat caaccaaaga atgggataaa
     5161 ccaggatgaa aacaacaagc tgcatcttaa aaattttcac aaagatacag caatttctat
     5221 tttaagaatg ttgaattaac aaaagctaaa gaaacaatta ctatttttac ccaacttaaa
     5281 ttcaaagcca tgaatattgc tctaattgtg ctcattgcag ccaatgtttt aatcagtttt
     5341 aaagcatttg aagatcagtt tttcttcaga agatacgaat ttcacatcgg cagcattcgt
     5401 gccggagaac acattcgcat gttttcctca gcattcctcc acgttgacat tgctcatttg
     5461 gcttttaaca tgattacgct ttattttttc gctcccgttg tttctagtta tttaggtatt
     5521 ccttctttcc ttgtcgttta tttcggaagt ttattatgcg gaagtctact cacgttgcaa
     5581 tttcataaaa acgaatataa ctaccgtgct gttggagcct caggagccgt tacgggaata
     5641 ctctatgcag caatcctgct cagacctgac atggatttgt atttcttttt cattccgtta
     5701 cccattcctg cttatatctt cggaatcggt taccttttgt attccatcta cggcatgaaa
     5761 tcccgaaacg acaacatcgg tcacacggcc cacttcggcg gagccatcgg cggatatgta
     5821 attaccctaa tcaaagagcc tcaaatgctc accgagaata ctttgatggt tggtttgtta
     5881 gccattccaa ttattatttt atttattatg gcaaaaatgg gcaagattta aaacgatttg
     5941 gcacacaaat tgaataaagg aatttagata aaacatactt accatgaaaa aatcaataat
     6001 tattctgttt gcagttgcaa tgacaactat gaatgctcag gaaaataaat tagttcctca
     6061 aatatcagtt tccggagaag gaaagatgaa agttactccc gaccgagttg ttgttaattt
     6121 cggagtagac aacgttggta aagacgccac cgaagttaaa aaaatgaacg atgaaacagt
     6181 ggacaaagtc attaaattca ttaagaaatt cggtattcca acagctgatt ttcaaaccac
     6241 gaatgtgagt ttacacaaaa catacgacta cgataaaaag aaacattctt atcaagcgtc
     6301 gcaatccatc accatcactt taaaagacat cacaaaatat gatgaattaa tgatgggatt
     6361 agtggacacg ggaataaaca acatcagtaa tgtagagttc aagtcctcaa aaatggaaga
     6421 actcaaaaca gaagcccgaa agctagccat tttagatgcg aagaaaaaag cagaggatta
     6481 tgtttctgtg ctcaatcaaa aagtaggcaa agctatttta attacagaca attcgcaacc
     6541 ttactatcca ccgatgtata aaaacatggc ctatgctgag atggcaacag atgcaggttt
     6601 agacagagaa acactagcca ttggtgagtt agaaattatc actaatgtaa gtgttagttt
     6661 tgtactggaa taattgatat aataagctat tataaaacca cgaatgagat aattcgtggt
     6721 ttttttattc agttatttgt tttttcaaat gttcactgtc ccaagcatgg tcagttgctg
     6781 aaaagttaat gtgtaaatcg tttttaagta attgctcctg catttctaat tcgtctttca
     6841 cggtggctaa ataggccatt ttatcgtgtc ttttagcagc taattttcga atagcggctt
     6901 cgtcatactt aataaaacgt tgaccttgtc tggtggctga gtattttcgg tgaccgagtt
     6961 gggtgagagc atccacccct aaatgaacag ccgtataaag tgtttctcta tagattttat
     7021 ctaccccgtg atcgattaat tcataggcat ccactcggtt tcttgctcgg gtgaggatat
     7081 ttaaattagg gaaatgtttt cgcaataatt ccacgacact taaattaact tccgggttat
     7141 caatggcggc aatgaagatt ttagcattct ctgctcctac agctttcaat aattctaatc
     7201 gggtagcatc tccataataa actttaaacc ccattttacg caacaaatcc actcggtctg
     7261 aatcgtgatc caagatagtt gctttaacgc cattggcttt taannnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnccactcg gtctgaatcg tgatccaaga tagttgcttt
     7441 aacgccattg gcttttaaca atcgaccaat tgtacttcca aagtgaccaa atccggcaat
     7501 gataacttca ttgttttcat caatgatatc gtgcggtttt tttacttttt cttttacacc
     7561 aaagaacggg tcaatccact tttcattaat caacaataaa aaaggagtgg aaatcatact
     7621 caaagcaata atggccatca tttgattggc taatatgctg ggtagaatat tcaattgcat
     7681 ggagaagctt aaaatcacaa atccaaattc tcccgcttga ctcaacccga aggaaaataa
     7741 caaattttga tcgttacttt ttttataaaa tttaccaatg ccaaaaagaa caaagaactt
     7801 aatggatgtc aaaatagctc caaagattaa aatataagca ggatcatcaa tgatcaattt
     7861 aaagtttatc gatgcaccaa ccccaataaa aaacaaccct aaaagcaatc ctttaaacgg
     7921 agcgatatca ccttctaatt catgtcggaa ttcggagtta gctaatacga ctccggccat
     7981 aaaggctcct aacgcaggac tgagtccgac caattgcatt aaataggaaa cggccatgac
     8041 taacaatagg gcagaagctg taaataattc ttgtaaacgt gttttagcaa cgatgtgtaa
     8101 taacggaaca actatgtatt tccccgcaaa atagacggca atgattgccg ctagtacaat
     8161 taaggtttga atccaaccgg gcaggtctat gattaaggag ctttttaaat catcatccgg
     8221 aatagtttgt cccgacatca tcaatggtat aatagccaaa attggaatca ccgcaatatc
     8281 ctgaaataac aaaaccgaaa aggacgctcg ccccatagaa gtttgcgata accctttttc
     8341 tttcagcgtt tgcaacacaa ttgccgttga cgaaagcgat agtgccaaag ctatcacaac
     8401 ggtggtttgc caattccaat ctaaaaacaa ttgacacaaa ataaaaatcg aaacacccgt
     8461 tccaattaat tgcatagaac ccattcctaa aataaacttt cgcattttcc agaatttata
     8521 agggtcaagt tccaatccaa ttaagaacaa catcatcact actccaaatt ctgcaaaatg
     8581 catgatgtct tctccttcag acccgataaa gcctaaaaca aaagggccaa taactattcc
     8641 ggcaaacaaa tagcctaaaa tagaacttaa acctagtttt tttgcaatgg gaacacaaac
     8701 cacagcggag gttaaataga caatggcttg tataaaaaat cctttatcca tgtttttttg
     8761 atttagaatt aaaccattcg tttaagtaaa aatgggtatt catttctttt atgtctaatt
     8821 catttgattt taaataaagc aataaatcag cataaatttt gccataattg tcagcctctg
     8881 attcattcat ttgatgtgtt ccgtgaatca cgaatggagg cagatagtgc atgtgacaaa
     8941 cttttgcggt ttggttaaag ggttctaata attctggaat agaataacgg tcgcggcctg
     9001 cctcacaata gttttctttt tttccaccgg ttgtaatggc ttgaaagatt agtttgtttt
     9061 gaagagcgat tccgttttta ccgtaagccc aatcatattc caacaccatg tcaatccact
     9121 gttttaaaag gggaggacaa ctataccaat acaacggatg atgccaaata ataatatcat
     9181 gtttttgaag taaagcctgt tctcttttga catcaatgtc aaattcagga tattcttcat
     9241 acaaatcatg aaaggtgatg tttttcgact ttggaatatg ccgaactaaa gcctcattga
     9301 caaaagactt ttcaaacaac ggatgagcaa atagaattaa tattttgttt tgagccatgg
     9361 tttaaaaata cgtctttttt ttcgaaaaaa gaagtgattt agctacaaat caaattatat
     9421 tcctaaataa ttggagggtg ttatttgcag taattccgct ttgatagttt ctgaaacggc
     9481 taaagaatga ataaattcat gtatcgtttt ttgattcatg acctcgttcg tgcgagttaa
     9541 acctttcaat gcttcatacg gatttggata gccttctctg cgtaaaatgg tttgaatagc
     9601 ttccgccacc actgcccaat tgcgttctaa atcttcttcc aattttgaac ggtttagcaa
     9661 taatttattt aatcctttca aggtagcttc aaaaccgatt aaggtatgtc caatcggcac
     9721 accgatgttt cgtaaaacgg tgctgtctgt caaatcgcgt tgcaaacgag agagtggtaa
     9781 ttttgcagaa aggtgttcaa agatagcatt agccattcct aaatttcctt ctgaattttc
     9841 aaaatcaata ggattcactt tatgtggcat agctgaagaa ccaatttcgc cggctttgat
     9901 tttctgttta aaataatcca tcgaaacata cgtccaaata tctctgtcta aatcaatgag
     9961 tatagtattg atacgtttca agcagtcaaa ataggctgca atatgatcat agtgttcaat
    10021 ttgagtcgta gggaaagaat gatgtaggcc taatgtttct tctacaaaat gggttccgaa
    10081 tttcttccag ttaatttgag gataagcaac atgatgggca ttataattgc cggttgctcc
    10141 accaaattta gctgcaaagg ggatattaaa taattgacgc atttgttctt ccaatcgttc
    10201 tacaaataca cctaattctt ttcctaatcg ggtaggagag gcgggttgac catgtgttct
    10261 ggctaacata ggaacgtctt tccattcctg actcatgtct tttaatttgg aaatgacact
    10321 gattaaatgg ggtaaatata ctttttcaaa actctctttg gtcgaaagag gaatggctgt
    10381 attattaata tcttgtgaag ttaatccgaa gtgaataaac tctttgtagc tttccaaccc
    10441 taaagcatca aaaccatttt ttatgaagta ttcaaccgct ttcacatcat gattggttgt
    10501 tttttcaatt tctttgatgg ctaaagcatc ttgtgtagaa aagtttcggt ataaatctct
    10561 taatttttca aatacggaag gatttacagt acttagctga ggtaatggca gttcgtataa
    10621 tgcaataaaa tactcgattt caaccaacac gcggtaacga attaaggctt cctcagaaaa
    10681 ataggcagca agagaaactg ttttatttct atatcttcca tcaattggtg aaatggcgtt
    10741 taattcgttt aatgcattca tgtgttgtgg tgttggtttg aagtgcaaag ataagtttta
    10801 taaggcagaa ttggaaatga aaaaaccgct acttcaaata aattatcttt attataggtc
    10861 taaaagacgt tgttttaata cggccattcc ttctgaacca ttcattggaa ttatttctaa
    10921 cggattagcc aattccaata cagaagcagg attggggtca atgtaaaaaa caggagtttc
    10981 gggttttgta taatgcataa tcccggcagc aggataaact tgaagtgaag tgcctactac
    11041 cacaaaaaag tctgcttgtt cggctaaatc aattgcttct tctatggcgg gaacatcttc
    11101 accaaaccat acaatgtggg gacgtagcgg aaaaccgttt gggtcggtgt gatttgacag
    11161 taaatcatcc tgccaatcta agatgtattt ttcgtctttt gtacttctta ctttgagcaa
    11221 ttcaccatgg agatgcaaca cgtttttact gccggccttt tcgtgtaaat tatccacatt
    11281 ttgggtgata atatgcacgt caaaatgcga ggctaattcg gctaatataa aatgtccttg
    11341 attaggattt acctccagta attgttttct tcttttatta taaaaagcta aaaccagttc
    11401 cggatttttt ttccatccta ttggtgaagc aacttccata atgtcgtgac cttcccaaag
    11461 gccatcggca tctctgaacg ttttgatgcc actttcagca ctaattccgg caccggttaa
    11521 aacaactagt tttttcattt tttactttat tacacctttc ccaacgtata caaattttct
    11581 aatgttggcg tagtactacc accatcaggt tctaaagtaa ttccaaaagc ctcagcctca
    11641 ataacattat cgaccggaaa aatcaacatg ctttttcctt tgaaattatc taacaatcca
    11701 atactcaacg gtgttaaggg atttaatttt aaagcccaaa cctgataaac cattccttcc
    11761 ggaggttcag gtaatccggc ggcatcaaca taaacagtat gtgtttcttg attccaatat
    11821 acttttgcat atgcttcagg ggaaacttct tgtccgccca aagcaattat tgtatttttt
    11881 gtatctcgaa caaaggaaag catatcttcg tttaccttat tttttgaaac caacacagta
    11941 tattcttctt tcattttctt tttgtcagat ttaagcgaga gaacatcttt cttagttata
    12001 tccaattgtt ggtataaata agcaccgcct aacagcaata ggattgctgc agcccaacct
    12061 aaatattgac tgatattgga tttttttggt tcctttttta tttcgacaac gttgggttta
    12121 ttctcaaaaa tttgctgctt gatagcttca taatgctgac ctgataaatg cggtgcaaca
    12181 cttccggata acaaaatcac tgttttttca atagcaacta tttcttccct gatttctgaa
    12241 tgttgttgtg ccattttatg aacttcttcc gtttccagtt catttaattt accgtaaaca
    12301 tacagttcca gaatgcctga ttcgatatat tcttgtgtac tcatgatacg tgtaataagt
    12361 ttcttaattc gctaatgcaa tttctgttat gtgttttgac ggttcctaaa ggtatttcca
    12421 attcatctga agcttcttgt tgtgtaaatc ctttaaaaaa taataaatcg attagtttta
    12481 tacatcttgg ctttaatttt tttacaaatt cctgaacgcc aatagtgtca attttgccgt
    12541 tcattcgagt ttggtcatct aataaatgca caaaattatc agcggaaatg ttttttaaac
    12601 tggtactaaa tccttttgaa cgtaatttat caagggcaca attgcgtgta atggttacca
    12661 tccaagagta taatcgccct ttgttctcat cataagtatc gatgtttttc catattttta
    12721 taaaggcttc ctttaaggca tcttgtgcgt cttcggggtt tttcagtagt gttgctatta
    12781 tcccaaaaag acttttagca tacatatcat acaaaagagt aaacgccttg tcatcttttt
    12841 tgtaaattag gggcaataat tcttcctgag tcataccttt ttattttatt gactaaggta
    12901 ataacttagt gtttaaaaaa caattttgaa tgaatgaaat gttaaaaaca attgaatacc
    12961 ttactttttt agaaggattt ctaactgaaa acagaaagtc taaattttta gaaattcttc
    13021 aacttcgaac gcaacacttt accgtagcga ttgaagatgt atttcaaatg cacaatacca
    13081 gtgccgtaat gagaagctgt gaagtttttg gtgttcagga agtgcatgtg gtagaacaga
    13141 aatatggaaa gaaaattgac actgaaattg ctatgggagc tcaaaaatgg gttgatatca
    13201 aacaatatcc ctccattcaa aattgtatct cgggtttacg ggagaaagga tatcaaatta
    13261 ttgccactac tccgcacacg gattcctgtt tgttagaaga ttttgatatc tctaaaaagg
    13321 ctgctttctt ttttggaact gaaaaagatg gtctttcaga ggaagttatg caacaagcgg
    13381 atggtttttt gaaaattccg atggttggtt ttacagaaag cttaaatata tcagttgcag
    13441 cgtccattat tcttcaaagt gttacgcaac gacttaggaa ttcttccata aactggcaat
    13501 tatcagagga ggaaatcttc ttaaaaagaa ttgattgggc taaaaaaaca attaaagact
    13561 gcgatagagt tgaagaacga tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nngaattgat tgggctaaaa aaacaattaa agactgcgat agagttgaag aacgatattt
    13741 ggaggggtta agcaataaat aaggaaacca acattcgtaa ttcgtaattt ataattcgta
    13801 attaattttt actttctttt caaaatctta tactcttcat aacaatcatt gatagcatcc
    13861 aacacttgaa gatcattggc cgttttgata aattgttctg aataattgca gcgttctaat
    13921 atctcggcga tatctttttt gttaactcca agcatgattg caatggtttc ccggtcaaat
    13981 tttgaagcaa gtaagttacg aactgtatcg tctttattca tttcttttaa cttgcggagt
    14041 tctttgggtt tgttcccgaa gaaattataa agcatgtcgg tcggattaaa gatagaaccc
    14101 aacacgcgat taaatgcatt tggtgaatat tcgccggctt cataaccata aggcaatccc
    14161 gaaataccat accgatagtt ttctttcacc ggaattaatt tagcatctac ctgaagataa
    14221 ccggtaagat tataaggtgt tagcacaacc tcttctaatg cgattgcttt ttcagtcaaa
    14281 ctgatttggg ttattttcga cttaatccag tcattcgtta cacgaacccg aatggattga
    14341 aaaccaatta atgaaatatg taatgtatcg tttacctgca cattaatttc aaacacacct
    14401 ttatcatctg taatcgttcc ccgaacagaa tttaaattga tgatgtttac gtttggtaac
    14461 ggttttgatt tagtgtcatc taacacagta ccgctaactc tctgagttcg ctctgcaatt
    14521 tgtgctgtga ctgaagaaag ggataaaaac aataaaaaaa ctacaaaata tctcataaac
    14581 ggttttaacg gtttaaaagt aataaatcac ttttagatat tttgtagttt cagtataatt
    14641 ataagaaaat taacaaaagt tagttagctt cttcttggtc ttcttgtctt tgcagcttca
    14701 aaagagcggc ttgcaggacg ttcgttgcgt tctccacctt ctgaacgtct ggctggtctt
    14761 tctgatgaac taccttcggt tctaggtctg aatccgcctt ctttgcgatc tcctcttgga
    14821 gcagcactac gttctgttct tgaaccgaaa ccacctgaac ggggacctga atttctttct
    14881 cctctgaatc ctccgccacc gcctgaactt cttccattgt ggtcacgtct tccgccacca
    14941 tcatttttag aaatttctac gttgatttta cgaccttcca ataacattcc gtttaacaca
    15001 tccattactt tatcagcgtg ttctgcatca gtattaaaga aggagaaacc ttcttttaca
    15061 tccactttat acacatcttc acgacctaat tctaacgtgt cgcgtaaaaa gtctttcagt
    15121 tgcatccaat cgaaattgtc acgtgaacca atgtttacaa agaaacgggt tgctccactg
    15181 ttgttttctc ttggttctct gtcatcacgt tcgcgtcttt ctgaaccgga tgattgatta
    15241 gagatgtctc tgttcttttt ataataatta atgaatcggt tgaattctac cgataccatt
    15301 ttcttgatta attcttcttt gctcaatccg tttaaaactt cgttgatagc cggtaagtag
    15361 ttgtcaattt catgatcaac ttcagtatct ttaattttag tggctaagtg caataattga
    15421 atttcacaga tttcaatacc ggatggtatt gttttctctt caaatttctg tttaatgata
    15481 cgctcaatag cagagatttt acgtaattca cttttagtaa caattacaat ggatgttcct
    15541 aatttaccgg cacgacctgt tcggcctgaa cggtggttat aggtttcaat ttcatccggt
    15601 aattgataat tcactacgtg tgtaatgttg tccacatcaa ttccacgagc ggcaacatcg
    15661 gttgctacca acatttgaat ttgtcttcct ctaaacgatt tcatcacact gtcacgttgt
    15721 gcttgtgata aatcaccatg caaagcagcg gcattgtatc cgtcttcaat tagtttttcg
    15781 gcaacggctt gtgtatctct tttggtacga cagaaaacaa ccgagaagat atcagggttt
    15841 gcatcggcca aacgtttaag agcttcataa cggtcacggg cattgattaa ataaaactca
    15901 tgagatactg ttgcagatcc tgagtttttt gttcctacgg taatttctac cggttcgtgc
    15961 atgaattttt ttgcaatacg cgacacttct gccggcatgg ttgctgagaa taaccaagtg
    16021 tttttctcgt ctggagtggt agataaaatg cttacaatgt cttcatagaa tcccatgttc
    16081 aacatttcat cggcttcgtc aagaatacaa tagttgattt gggtaatgtt taccaaacct
    16141 ctattaatca tatcttgcat tctacccgga gtggctacaa taatttgagc tcctcttttt
    16201 acttctctgg cttgttctga gatgcttgca cctccgtaaa cagctactac gttaattcct
    16261 ttttcgtact tagagtacaa tttaatttca ttcgtaatct gtaaacatag ttcacgggtt
    16321 ggagataaaa ttaaagcctg cgtatttcta ttgttcgcat caattttttg gataagcggg
    16381 aaaccaaatg ctgccgtttt ccctgtccct gtttgagcca acgcaactaa gtctgtttct
    16441 tgttccaata agaggggaat cgctttttcc tgtacttcgg tcggattttc aaatcctaga
    16501 tcgataatcg cacgctgtag cgattcactc aatcctaatt gttcaaattt attcatataa
    16561 tattttaaat tgggtgcaaa ggtacagttt aaaaaccata taaactaatg atgttaccta
    16621 ttgattttta attagttgga gtattttgga tcaggatagg taattggtta tgggtaatag
    16681 ggatgaaaat cgagaaaaaa atgtgattat ttgatgcctt tttgttttaa aaacgcaacc
    16741 aatttttcaa ccgccaaacc gcgatgactg atttttgatt tttcgctcat agacatttct
    16801 gcaaaggtgt ttgaataacc attgggtata aaaattgggt cataaccaaa acctgctgaa
    16861 cctctttttt cgtggatgat ttctcctttt ataattcctt caaataaatg ttgattacca
    16921 tttagattta aacagattac ggttttaaat tgagcttttc gatttgtttt gtctgaaaga
    16981 ttgtcgagta gttttgccat attatcatca gcattctttt gctctccggc ataacgggct
    17041 gaataaactc ccggggcgtt atctaaagcg gctacttcta aaccggtatc gtctgcaaaa
    17101 cagggatagc cgtatttttc agtaacataa ttggcttttt gaatagcgtt gccttcaatg
    17161 gtgggtgctg tttccggtat atcttccagg caaccgatgt cttctaaact taagatttgt
    17221 agggtgtttg gaagcagtaa ttgtatttct tttattttgt ttgggttgtt tgaagcgaat
    17281 actaatggca tgggttcgtt ggtttagtta ggctgttttt taacgtagtc ttcaatagct
    17341 tggttatcaa tcatgacctt cttaatcact ccggttccgt tgctgatggc tacttcagcg
    17401 tagactgatt tcttgacatc gtttacgctt tctgcatagg tttgttcagc catgggagct
    17461 ttgtattcgt tcatgtaata acggtcaaac ggataggaga cactgatctt ttgtattgaa
    17521 tcataattgt tggcgtaatc tacggtgagt tctaaatagt cggtgcgttc tttcggggct
    17581 gcggtgtaaa ttttatccat cacggcatag cctgttgtgt cttgtttaaa ggtggcataa
    17641 atggtttgat tatactgata ccgattggca tttttaacgc ctattgtgct attcgcaaag
    17701 cgtaaggaga tatattttcc gcggaaggga tcataggggt caacgggttc acatttgaat
    17761 ttcataatgg ttcccgtatc taaaatgttt tggttttgcc atatcatttc cgtggggaca
    17821 tagagttgtg cggcggctac cagcagaaag agaccgaaga gtagggcttt agttttcatg
    17881 ttgttttctt tttttgagta agaaatagtt gagagcaaag aacccgaaac cgacaattat
    17941 gaacatgact ccgcgaacaa tgaaggtcat gttttcgtca aagaagcggc atccggtgag
    18001 gatggctatg atgagtattc cgaaattgag tcgtgctatg ctgtgggttt tgttgccttt
    18061 ctgaatttct tgaagtccga gtgaaaggat atagaggttt gacaaaatga cggtggcaat
    18121 tatgcttact tgggcagtga taaaaaacac agcaagggct ataaagttaa aatggaatag
    18181 cgtgaacggg gttgctgctt ttcttttgtt gagctgatag agtaagaggg cacaacccac
    18241 aaacaacgta agggtgacgg ttagtaacaa aggttcgggg gcgaggtttt tgagtaggag
    18301 tggtttccaa ataaaacgga aactggccat gtacaatagg gttaaaccgc ctagtttacc
    18361 gatggttaaa tagctgtttg caatggtatg gtggtgacga aagtaggctg tttttccgag
    18421 gaggtagaag agggcaatca agaaaccata ggtgataaaa atgagttcgt tgttgttttt
    18481 gtcgaggctt tcgaggttga tgagtagggt gagggcaata aaccaatggt ggatggtggt
    18541 gaagttgccg ttgggttttt gtttaattaa ttgatagtag tgcggtgcta cggcaagcag
    18601 gagcaaccaa tacttataat tttgaacatt cgggtagtca atgtagccga tggtcatgtt
    18661 gtagacagtg atgccaatga ggaagagtag ggaaacgacc gacgatttca agagataaat
    18721 gagtggcaac gcgagcaaca tccaggtgag gacgaagcga ttgaagttgc cgggaaggtg
    18781 atagatttgg gcaattaagg cgatggatgc tccgatgcca cagaagagga aggctccgct
    18841 gctttcacgc catgctattg attcgggttt ttttaggatg gtgtatccgg aggcgatttg
    18901 tgctaggagg agtggcagaa aagcaaggac tacttgtgtg gtggtggtga actcatccca
    18961 gttgtggccg atgaggagga tgatgccgag gcctccgagg agtgctccga agatgccgaa
    19021 aatgatgggt aattttgagg cggttgtttg tttgttttgg taatagtttg ttatggcggt
    19081 ggctacttcg ggggtgatga tgccgtgttc gagtagttcg ttgaggtgtt gtttttgcat
    19141 gctgtttttg tgttgcggtg tt
//
LOCUS       DAOTCW010000039        18034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1716_length_18034_cov_45.242171, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000039 DAOTCW010000000
VERSION     DAOTCW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 18034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18034
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1716_length_18034_cov_45.242171"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /locus_tag="P5335_04905"
     CDS             complement(<1..1627)
                     /locus_tag="P5335_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRZ74252.1"
                     /translation="MKKISLLLFCLAFSFLTAQVNRIEPPNWWIGFKNENLQLLVHHP
                     NIGTTVPKISYPGVSIIKVNQAKSPNYLFIDITIDKSAKPGTFNIVFEPEKGKKIIQS
                     YELKAKSKPSEDFIGFNSSDVIYLITPDRFANGNEANDVVKTMKETTLDRSQDYKRHG
                     GDLQGITNHVEYIKNIGFTAIWPTPVLTNDMKGGSYHGYAMTDFYQVDPRFGTLTDYK
                     NLADKLNQNSMKLIMDQVANHCGSEHWWMKDLPFADWVNYQELFEKEKKFITSNHRRT
                     TNQDLYASQKDKKEMTDGWFVSSMPDLNQRNPFLAKYIIQNSIWWIETLHLGGIRQDT
                     YPYSDKAFMANWTGEIMAEYPNFNIVGEEWTTNPLLIGYWQKGANNKDGYQSNLRSTM
                     DFAMQTIIPQAIKEPESWDKGLAKLYDGLGNDFHYASPKDLMIFPDNHDMDRIFTQLN
                     ENIENTKMALATYLVLPRIPQIYYGTEILMQNTAKPGDHGLIRTDFPGGWKGDKVNAF
                     TGEGLSVEQKDMQNYLTKILNYRKNSKAIHNGKT"
     gene            complement(1705..4008)
                     /locus_tag="P5335_04910"
     CDS             complement(1705..4008)
                     /locus_tag="P5335_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HRZ74253.1"
                     /translation="MNQDYIIPDNWSIIEEGFEQDLVKSSESLFSIGNGAMGQRANFE
                     EHYSGKTFQGSYIGGIYYPDKTKVGWWKNGYPEYFAKVLNAPNWIGINIEINGENLDL
                     AKCVAVKNFRRELNMKEGWYHRSFIATLQNGTEIEVQVRRFLSIDLDEVGVINYEITA
                     LNQEAKIVFKPYLDAGVHNEDANWEEKFWEPLEVKNNGNEALVTARTFKTHFTATTFM
                     QNTILVDGISLNASPFNIASSNETIEFSYEVSISKNQTTTIQKIGGYTVSMNHENTQI
                     AAQKAIADALKIGYDKLLENQIKAWAKIWEMSDITIDGDVKAQQGIRFNIFQLNQTYL
                     GKDSRLNIGPKGFTGEKYGGSTYWDTEAYCIPFYMATKDQQVARNLLTYRYNQLDKAI
                     ENAGKLGFKNGAALYPMVTMNGEECHNEWEITFEEIHRNGAIAFAIFNYYRFTGDYSY
                     IPEKGLEVLIGIARFWHQRATYSTHKNQFVILGVTGPNEYENNVNNNFYTNYIAKWCI
                     DYTVEQINKIEKEYTSDYTRILNKVKLSQSEINHWKEVADNMYFPFSEEHDVYLQQDG
                     FLDKELVKVHDLDKSQRPINQKWSWDRILRSPYIKQADTLQGFYFFEDHFSKAQLEKH
                     FDFYEPFTVHESSLSPCVHSIQAAGLGRMEQAYTFYLRTSRLDLDDYNKEVHEGLHIT
                     SMAGTWMSIVEGFGGMRVKNSTLHFEPKIPTQWKGYSFKINFRNQVLEVSVRPNETKL
                     SLEGDKEIIVFVNGKEILVEPNSLVTV"
     gene            complement(4012..4668)
                     /gene="pgmB"
                     /locus_tag="P5335_04915"
     CDS             complement(4012..4668)
                     /gene="pgmB"
                     /locus_tag="P5335_04915"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HRZ74254.1"
                     /translation="MNQKCFIFDLDGVIVDTAKYHFLAWQKLAQQLGIDFTPEHNEQL
                     KGVSRVRSLDIILSLGNFEASQEDKNKWLVQKNEDYLSYLVNMDESEILPGVIPVLAF
                     LKEKNQKIVLGSASKNAKPILEKAKIIDFFDAVVDGNDVSNAKPDPEVFLQGAKKVNF
                     PPEKCIVFEDSVAGIQAANIGGMTSVGIGDESVLNEADYVFPDFTHIDLNFIENLVNK
                     "
     gene            complement(4679..6007)
                     /locus_tag="P5335_04920"
     CDS             complement(4679..6007)
                     /locus_tag="P5335_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HRZ74255.1"
                     /translation="MKKPKLSFWQIWNLSFGFLGVQIGYSLQNGNTSRILEALGADVH
                     SIGFFWLAAPLAGLFVQPIIGLSSDKTWTRLGRRVPFILGGAIISALAMYFMPNAEYF
                     TDLLPALAFGAIMLLLMDTSFNVTMQPFRALVGDMVDDDQKNLGYSVQSALINFGAVF
                     GSLLPYILSKLNVANIPAEGEKVADSVVWSFYIGGTILLLSVLWTVYKTKEYPPKEHA
                     EYNNIDLEANENKPKASIWSLIPNAPKIFWQLGIVQFFSWFSLFLMWVYTTRAIANQV
                     WGDEALDPKSIAFNEAGNWTGVMFAFYSGIAAVFAFLIPSLAKKYGRKNVYSFSLIMG
                     GLGLASIFFVHDKTILLVSMIGVGMAWASILAIPYAMLSSSLPAEKMGVYMGLFNATI
                     TLPQIAAGLIGGSIIALFDNSPVAIIVISGMSMILAGLSVYLVKEKSSVH"
     gene            complement(6208..>6581)
                     /locus_tag="P5335_04925"
     CDS             complement(6208..>6581)
                     /locus_tag="P5335_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRZ74256.1"
                     /translation="ENLILKIEDIDHCDEVIENLINSQPIEAIFAVNELFAVTAIKAA
                     LKKGLKIPEDIAIIGFTDGIISKYSSPSITTVSQNGIKMGGKAAKMLIERLESEDEDD
                     EHYRTEVIETHLVERESTPTL"
     assembly_gap    6582..6681
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6682..6983)
                     /locus_tag="P5335_04930"
     CDS             complement(<6682..6983)
                     /locus_tag="P5335_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRZ74257.1"
                     /translation="MKRKVTLKQIAKELDVSISTVSKSLKDSSEISEDTRQKVQAFAK
                     LYNYKPNNIALSLKNKKTKTIGIIIPEIVHHFFATVISGVENVANEHGYNVIVCLS"
     gene            7210..10179
                     /locus_tag="P5335_04935"
     CDS             7210..10179
                     /locus_tag="P5335_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRZ74258.1"
                     /translation="MKTISKKLLFLLLLLPLSVLAQSTVSGVVTDKGSAQPMPGVNVI
                     IKGTTKGATTDFDGKYSLSNVKSGDVIEFSYVGFKPVDVTYTSQKSIDVSLEEESAKL
                     EEVVLVGYGTVKKKDATGSVVQVTTKDFNKGANVTAENLLNGRVAGVTINTSGQPGSG
                     SAIRIRGGSSLGASNDPLIVIDGLPIGEVAGGATSVLASINPNDIESFTVLKDASATA
                     IYGSRASNGVIIITTKKGGKELAVEYNFQYGSGEVANKVDVFSAEDYYKMVEANRPQF
                     LNRLGVDDPNNPGEKIYYDTDWQDEIYRRTDLVENTLTVKGSLFKKIPTRLSVGNTYQ
                     EGLRLTSSFNRNSANLAMNPNFFEDHLKLKLNANLSNENNRFADGVEGGAIRFDPTKP
                     VYDSNSAYGGFFQYTDANGLALNGVNRNPVAALLQRKNITEVNRIFGNFELDYKLHFF
                     EDMRAVVNVGWDKSKGSGSNELSKESPDGQRNGNTTAILTNGSYTEFTSERENTLLDA
                     YLVYSKSLENFRAEVTGGYSYQKFTGSSFSSKDYRRFNDAGNQNNYYTETDVVLIGFF
                     GRANLTFNEKYLVTLSYRRDGTSRFSEDNRWGNFPAAAIAWKIKDESFLKDSKTFSDL
                     KLRAGWGITGQQDLGASRFDYLSTMSTGQPGNDYMFGGEPINPGFPNYTNPDLKWEET
                     TTYNVGIDYGFWNNRITGALDFFYKQSDDLLARAPVPDGSNFSNVGSQNIGAFTSQGV
                     EFAINAALYQGDGFNWDINFNATHFNTNIDKLAAGIDIEVGGVGSGTGGTIQVHSEGF
                     TPYSYFVYKQLYDTAGAPIEGAYADINRDGIITAADRYIYKNPDPEAVLGFASNMSYK
                     NFDFSFNLRASIGNHLYNAVNAGNAQYDNIESTQVVANLPTAVLDSNFNTTSDVLFSD
                     YYIENASFLKMDNITLGYTFSKIFNDKATLRLSTGMQNVFVITEYSGLDPEVFGGIDN
                     TIYPRPRTILFGANIKF"
     gene            10188..11819
                     /locus_tag="P5335_04940"
     CDS             10188..11819
                     /locus_tag="P5335_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRZ74259.1"
                     /translation="MKTKIKLLTAFSVLLFLGACTDDLNVTPQDDNLFLTDDFYTTDA
                     SYKQGLAGVYSNLSLTGTSGTGSSFIEGLDAGTSQYGRALWYMQVLAADEAVWSYEND
                     PGVREIQRNIWSAENPIVRGMFGRAMVQVSFVNEYLRQTTDAKLDARGVGEAVRAEIP
                     TFRAEARLLRALAYYHLMDLYGKAPFLTEADLPGFTAGPQYDRAQLFAFLEEELTAIE
                     ADLKAPRTNEHGRADKAVAWMILAKMYLNAEVYIGTPKYTECIAQCNNIIGGGYSLAP
                     NYLYNFMSDSDTNSAKNEIIFPLISDGQYTKNYGPTTVMINGEVGSLEQNGVSLGVGA
                     GGWGGALRVRKQFANKFEDALYNGDARNTLIKANRSAEIGSISDRDSGFVITKFSNRT
                     SNGGQGSDLTFVDTDFPLFRLADVYLMLAEAQMRNAGATNSSLDQTGIATQQSVDYIN
                     DLRERAFGNESANVSVSQVSLQFILDERLRELHWESHRRQDLIRFNRYTGGNYNWAWK
                     GNGSNGISISGNLKVYPLPSQSLAVNPNLTQNPGY"
     gene            11848..12939
                     /locus_tag="P5335_04945"
     CDS             11848..12939
                     /locus_tag="P5335_04945"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HRZ74260.1"
                     /translation="MKNTFKFLFAFIAFAGLMSCEDEENFQILDPSGDFVIVTPDAGT
                     NIVLDPNLQTNPSITLTWSPADYGTPTEIQYVVEFAAAGTDFAEPVVAASTTNTNVTW
                     NVLELNGAALSSGLTPFEEGGLDIRVKATTGTTGSQERISNMLTIFVTPFSTDLPKLW
                     LPGSYQSDSGYGNNWTQETAATLAASAYGNYDFEGYVYFASAQSAPDNGYKFTDAPNW
                     DNGVFGDDGSFSGALTSPGDNISANPGYYLVKANPSSASAGGLAAMSYTVTATEWGIV
                     GAATPNGWDSSTQLTYNSSTKMWEGVVALTAGEFKFRANQAWTINLGGDPAAMNYDGG
                     NLSVAAEGTYLVKLNLSNPRAYTYELIAQ"
     gene            13006..>13832
                     /locus_tag="P5335_04950"
     CDS             13006..>13832
                     /locus_tag="P5335_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HRZ74261.1"
                     /translation="MKKYYFFTFLFSLFSGLVFAQVTITPSTFNVTDQITITVSTAAQ
                     ACNLMGTSPAKVYMHAGIGDDTNAFGYSVIGTWGQDNGVGLMTNNGNGTWSITITPSV
                     YFGLNGTQQANATKLGMVFRNANGSQTLKLPPSCGDFIFNVGTFQLNLTSPVNNSSTI
                     INSGGSLVVSATNTGGNASYVLKANGNPINSNGATANYSFTHSTITSNQNYELVVTQG
                     SSTITRTFKVIVNPGTISQAIPAGMEDGINYNASDPTKATLKLNAPGKDFVYVAGSF"
     assembly_gap    13833..13932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13933..15087
                     /locus_tag="P5335_04955"
     CDS             <13933..15087
                     /locus_tag="P5335_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ74262.1"
                     /translation="RVAVLKEYADYSWSLDPNHYVIFEHLGADNEEQQWANYRINEGK
                     GVMMWGELWNPYKQLISGNSTNADITRMGHVSRGFTGKRLMGYAESHDKDRIIYEAVT
                     FGNGGGTAPVSGNLNNTIPRMKAIGATSILIPGPKMIWHFQELGMDDSIFTCSGGSSV
                     NTDYDAIPGDCKLDTKPQPQWVENWLGDALRNSVYNAYAKFISLKKNEPVFNGEYAIS
                     PNGSNIRQRIYVFNNSLPSTQLKNVVILANFSVASQNITPDFPYIGTWYDLMTDTPIS
                     VTSTTAQIALAPGEFKVYGNRLPTLSAETFELKDDIAIAPNPTTETFSVTVPTVKVEI
                     YSITGQLVKTFQNDFDTNYRFDVSDLTKGIYFVKVKDQNDREKSMKLLKN"
     gene            15194..17989
                     /locus_tag="P5335_04960"
     CDS             15194..17989
                     /locus_tag="P5335_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRZ74263.1"
                     /translation="MNKAGKKRIVKIVKIAGISLGTLLLLLFLLPTIFPETLTNQVKK
                     FANEKLNGELSFKETHLSFFTHFPSLTLSLDDFLLKGSAPFQKDTLIAAKEVAFGINL
                     KSVFFDEKIKIDKIFISEGTILIKVNKKGEPNYNVYVSEEAATVSDSTSASLKLERID
                     VKNTRLVYDDQSTKILINAKGFNYLGKGDLDKAVFDLKTKAQIESFDFSFENEQYLKN
                     KKVNAELITKINTNSLALVFEQNNLKINKLPVEFKGKFNFLKNGYDLDFSVESQNSQL
                     NDFFTALPPSYIKWLDKTEIKGKTDLFFELKGQYIASENKMPNVHFNMKIRDGYISHQ
                     KTPFPAENIFLNFDTKLPSLDPEQLAVKIDSIYFDVGEDFFNGNVIVNGLSKPKIDAR
                     IRSKMDLAKLDKALGIQNMDLKGTFTADIVAKGIYDKEKNQFPITKGDLELKNGHIKS
                     TYYPNPIENINFKANASNQTGQLKDLSLVINPASLQFEGKPIAMNASFQNFEDISYDI
                     KAKGELDIEKIYKVFSQKGLDVSGYAKMDVSFKGKQSDATNGNYNKLENKGILYLRDI
                     KTSSDYLPKPFIIKEGEFVFNQDKMNFKNFKANYGQSDFMMNGYMENVINFALSDSEI
                     LKGTFAVESNLIQINEFMAFSDNSKELPESAESEPVSQSGVIVVPTQFNFNLLAKANK
                     VVFDEMNIENLSGKMVVNQGKLALQNTSFSLIGIKVTMSALYFNESTNKADFDFKIKA
                     TEFNIKRAYNEIPMFREMASAAEYAEGIVSLDYKIAGKIDQNMSPIYPSLVGGGTLSV
                     KNVKMKGFKLFNAVSKKTETDALKDPDVSKVDIKTTVKNNLIKIERFKFKVAGFRPRI
                     EGESSFDGKLNIKMRLGLPPLGIIGIPINVTGTQENPIIGVGKQTENLEETEYEDGTS
                     PQVVPEQLPETIKDTIK"
BASE COUNT         5873 a         2934 c         3215 g         5812 t
ORIGIN      
        1 tggtttttcc gttgtgaata gctttgctgt tttttctgta atttaaaatt ttagtcaaat
       61 agttttgcat atctttttgc tcgacagaaa gtccttcgcc tgtaaaagca tttactttat
      121 ctcctttcca acctcccggg aaatcggttc tgattaatcc gtggtcaccc ggtttggctg
      181 tattttgcat gagtatttca gttccgtaat atatttgcgg aattcttgga agaaccaaat
      241 aggttgccaa agccattttg gtattctcta tgttttcatt taattgggta aaaattcgat
      301 ccatatcgtg attgtccgga aaaatcatca aatcttttgg cgaagcataa tgaaaatcgt
      361 tacctaaacc gtcatacaat tttgctaatc ctttgtccca gctttcaggt tctttaatgg
      421 cttgcggaat gatggtttgc atggcaaaat ccatcgtcga acgcaaattg gattgataac
      481 catctttgtt atttgcacct ttttgccaat agccaattaa caaaggattt gtagtccatt
      541 cttctcccac aatattgaaa ttagggtatt cagccataat ttctccagtc caattggcca
      601 taaaagcctt atcagaataa ggataggtat cttgacgaat gccacctaaa tgtaatgttt
      661 caatccacca aatgctgttt tgaatgatat attttgctaa aaacggatta cgttgattca
      721 aatcgggcat ggaagagaca aaccaaccat cggtcatttc ctttttatct ttttgagaag
      781 catataaatc ttgattagtg gttcttctgt gattggaagt gatgaatttt ttttcttttt
      841 caaataattc ttgataattg acccaatcag caaaaggtaa atctttcatc caccaatgtt
      901 cgcttccgca atgattagcg acttggtcca tgataagttt catgctattt tgattgagtt
      961 tatcagctag atttttataa tcggttaatg ttccaaatcg gggatctact tgataaaaat
     1021 cggtcatagc ataaccgtga taggagccgc ctttcatgtc gttggttaaa actggagtag
     1081 gccaaatagc cgtaaaacct atatttttga tatactcaac atgattggtg atgccttgta
     1141 aatcgccacc atgtcgtttg taatcttgac ttctgtctaa agttgtttct ttcatggttt
     1201 tcaccacgtc attggcttca tttccgttag caaaacgatc gggcgtgatg aggtaaataa
     1261 catcagaact gttaaaacca ataaagtctt cagatggttt tgattttgct tttaactcat
     1321 aggattggat tattttttta cccttttcag gttcaaaaac aatgttgaat gtacccggtt
     1381 tagcggattt gtcaatggta atatcaataa acagataatt tggactttta gcttgattaa
     1441 ctttgataat tgatacaccc ggatatgaaa tttttggaac agtagttcca atattgggat
     1501 gatgcactaa caattgcaag ttttcatttt taaatccaat ccaccaattg ggaggttcaa
     1561 ttcgattgac ttgagcggtt aaaaatgaaa atgcgagaca aaacaagagt aatgaaattt
     1621 ttttcatgat tgattgtttt atctcgcaaa gtcgcagaga cgccaagcaa tacttttgcg
     1681 acttagcgtc tttgcgagag tttattatac ggttaccaaa ctatttggtt ccactaaaat
     1741 ttcttttcca ttaacaaaaa ctataatttc tttatcacct tccaacgata attttgtttc
     1801 attcggacga acagaaactt ctaaaacttg atttctgaaa ttaattttaa aggaataccc
     1861 tttccattgg gttggtattt ttggttcaaa atgtaaggtg ctatttttca cccgcatacc
     1921 gccaaaacct tctacaatac tcatccaagt tcctgccatt gaagtaatgt gtaaaccttc
     1981 gtgtacttct ttattataat catctaaatc caaacgagaa gttcgtaaat agaaagtata
     2041 ggcttgttcc attctgccta aaccagcggc ttgaatcgag tgaacacagg gtgaaagtga
     2101 actttcatga accgtaaagg gctcataaaa atcgaaatgt ttttccaatt gtgctttact
     2161 gaaatgatct tcaaagaaat aaaatccctg taaagtatcg gcttgtttga tgtatggcga
     2221 acgtaaaatt cgatcccatg accatttttg gttgattggt cgctgacttt tatctaaatc
     2281 atgcactttc accaattctt tatctaagaa accatcttgt tgtaagtaaa catcatgttc
     2341 ttctgaaaaa gggaaataca tgttatctgc cacttctttc caatgattga tttctgattg
     2401 ggaaagtttt actttattta aaattcgggt ataatccgag gtatattctt tttcaatttt
     2461 attgatttgt tccaccgtat aatcaataca ccatttcgca atataatttg tgtagaaatt
     2521 attattgacg ttgttttcgt attcatttgg tccggtgaca cctaaaatca caaattgatt
     2581 tttgtgcgta gagtaggtgg ctctttggtg ccaaaaacgg gcaattccga tgagtacttc
     2641 caatcctttt tccggaatgt agctgtaatc acctgtgaaa cgatagtagt tgaaaatagc
     2701 aaaagcaata gctccgtttc tatgaatttc ttcaaaggtg atttcccatt cattgtggca
     2761 ttcttctcca ttcatggtaa ccattggata caaagcagct ccatttttaa agcctaattt
     2821 ccctgcgttt tcaatggctt tatccaattg attgtaacga taggtcaaca aatttctcgc
     2881 cacttgctga tctttggtcg ccatataaaa aggaatacaa taggcttccg tatcccaata
     2941 agtggaacca ccgtattttt cgccggtaaa tcctttcgga ccaatattta aacgagaatc
     3001 ttttcctaaa taggtttgat ttaattggaa aatattgaaa cggattcctt gttgagcttt
     3061 cacatcacca tcaatagtaa tatcactcat ttcccaaatt ttagcccaag ctttgatttg
     3121 gttttcaagt aatttgtcgt aacctatttt taaggcatca gcaattgctt tttgagcagc
     3181 aatttgtgtg ttttcatgat tcatggaaac agtgtatccg cctatttttt gaattgttgt
     3241 agtttgattt ttggaaatag atacttcata tgaaaattca atggtttcat tggaagacgc
     3301 aatattaaaa ggcgaagcat ttaaagatat accatcaacc aaaatagtat tttgcatgaa
     3361 agtagtagcc gtaaagtgcg ttttaaatgt tctagctgtt actagagctt catttccatt
     3421 gtttttaact tctaatggtt cccagaattt ttcttcccag ttagcatctt cgttatgaac
     3481 tcctgcatcc aaataaggtt taaatacaat ttttgcctct tgatttaaag cagttatttc
     3541 ataattaatt acacctactt catccaaatc aatagaaaga aaacgacgca cttgcacctc
     3601 aatttcagtc ccgttttgaa gtgttgctat aaaagaacga tgataccatc cttctttcat
     3661 gtttaattct ctgcggaaat ttttcactgc tacacatttg gctaaatcta aattttcgcc
     3721 gttgatttcg atattgattc caatccaatt gggagcattc aatactttgg caaaatattc
     3781 aggataaccg tttttccacc aacccacttt tgttttatcg ggatagtaaa ttccgccaat
     3841 ataacttcct tggaatgttt tcccggaata atgttcttca aaattcgcac gttgtcccat
     3901 cgcaccgttt ccaatgctga acaaactttc agaagactta accaggtctt gctcaaatcc
     3961 ttcttctata atcgaccagt tatctggtat gatataatct tggttcattt tttatttatt
     4021 cactaggttt tctataaaat ttaaatcgat atgggtaaaa tccggaaaca cataatctgc
     4081 ttcatttaaa acggattcat ccccaattcc cacacttgtc attccaccga tatttgcggc
     4141 ttgtattccg gcaacggaat cttcaaaaac gatacatttt tccgggggaa aattaacttt
     4201 tttggctcct tgaagaaaaa cttcaggatc cggtttggca tttgaaacat cgttgccatc
     4261 aacaacagca tcaaaaaaat ctataatttt tgctttttct aaaattggtt tagcattttt
     4321 actggctgag cccaaaacaa ttttttgatt tttttctttt agaaaagcca atacggggat
     4381 tacacccggc aaaatttcac tttcatccat atttactaaa taggataaat agtcttcatt
     4441 tttttgaaca agccatttgt ttttgtcttc ttggctagcc tcgaaatttc ctaacgaaag
     4501 gataatatcg agtgaacgaa cacgactcac accttttaat tgttcattgt gttcaggggt
     4561 aaaatcaatc ccaagttgtt gagccaattt ttgccaagct aaaaaatggt atttggcagt
     4621 gtcaactata acaccatcta gatcgaaaat aaaacatttt tgattcattg tagaataatt
     4681 aatgtactga ggatttttct tttaccaaat aaactgacaa tcctgcaaga atcattgaca
     4741 ttccggatat gacgatgata gccaccggtg aattgtcaaa aagagcaatg atacttccgc
     4801 caataagtcc ggctgctatt tgaggaaggg taatggttgc attgaataag cccatgtaaa
     4861 ctcccatttt ttcagcgggt aaagaacttg aaagcatcgc ataaggaata gctaaaattg
     4921 atgcccaagc cattccaact ccaatcatag agactaaaag tatcgttttg tcgtgtacaa
     4981 agaagatgga agctaaccca agtccaccca ttattaagga gaaactatac acatttttac
     5041 ggccatattt ttttgctaag gatggaatta aaaatgcaaa aacagcagca ataccactat
     5101 aaaaggcaaa cataacccca gtccagtttc cggcttcgtt aaacgcaata gatttaggat
     5161 ctaatgcctc atctccccat acttgatttg ctatagctct tgtagtgtaa acccacatca
     5221 agaataaaga aaaccaagaa aagaattgca caatacccaa ttgccaaaaa atcttcggag
     5281 cattaggaat taaactccaa atacttgctt tgggtttgtt ttcattggct tccaaatcaa
     5341 tgttattgta ttcggcatgt tcttttggag gatattcttt cgttttataa actgtccata
     5401 acacacttaa taataagatt gttcctccaa tataaaatga ccaaacaacc gaatcggcaa
     5461 ctttttcacc ttcggcaggt atattagcca cattcaattt tgagagaatg tatggtaaca
     5521 atgaaccgaa aacagcccca aaatttatca aagcactttg aactgaatag cccaaatttt
     5581 tctgatcatc gtcaaccata tcgcccacta gggctctaaa aggttgcatg gttacattaa
     5641 aagaagtatc catcaaaagt aacatgatgg caccaaatgc taaagccggg agtaaatcag
     5701 taaagtattc tgcattaggc atgaaataca ttgctaaggc agaaataatt gcaccgccta
     5761 aaataaacgg aacacgtctt cctaatctgg tccaagtttt atcacttgat aatccaatga
     5821 tgggttgtac aaatagaccg gctaaaggag ctgcaagcca aaaaaaaccg atgctgtgaa
     5881 catctgcacc taaagcttct aaaatcctac tggtattacc attctgtaga gagtaaccta
     5941 tttgaactcc tagaaatcca aagctcaaat tccaaatttg ccaaaaactt aatttgggtt
     6001 ttttcattat cgtaattgtt aattaatatt acgataagct atactgctta tcaaaactta
     6061 tgggttcgaa gtaaaaaata taagtcttga taattaatta caaatatagt ttttttttaa
     6121 tcggaaacta ttttttttta aaaattgtaa aacataataa aggcaattaa taaattaaaa
     6181 atcaataaaa tcgagtattg acatcaacta taacgttggc gtagattctc gttcaactaa
     6241 atgagtttcg attacttcag ttctgtagtg ttcatcatct tcgtcttcac tctctaatct
     6301 ttcgataagc attttagccg cttttccacc catttttatt ccgttttgac tcactgtggt
     6361 aatgctggga gaagaatatt ttgaaataat tccatccgta aaacctatta ttgcaatgtc
     6421 ttcggggatt tttaatcctt ttttaagggc agcttttatt gcggttacgg caaaaagttc
     6481 atttactgca aaaatagctt caataggttg actgttaatt aagttttcaa ttacttcatc
     6541 gcaatgatct atatcttcaa ttttcaaaat taaattctca tnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn ntctgataaa caaacgataa cgttatagcc atgttcattc
     6721 gcaacatttt caacaccact aattacggtt gcaaaaaaat gatgcacaat ttccggaatg
     6781 ataattccga ttgttttcgt ttttttgttt ttcaaactta acgcgatatt gttgggtttg
     6841 tagttgtata acttggcaaa agcttgtact ttttgacggg tatcttcact aatttcagaa
     6901 ctatctttaa gtgattttga aacggttgaa atggatacat ctaattcctt agcaatttgt
     6961 tttaaagtaa cttttctttt catgttgttt ttaagtcatt agtaatttgt gaataaagat
     7021 aattttattt ttttagaatc ggaatttttg actgtaaaat agcgaatatt gtaaaagttt
     7081 tcaacacgaa aacgttttcg gagcctattt aatgggtctt tgttcaaaac atcataaaat
     7141 ttccttaatt ttatacctcg aagtaaaaaa tataagctca aaacacaatt tttaacctaa
     7201 acaaactgta tgaaaacaat ttctaaaaag ttgttatttt tattgcttct actgcccctt
     7261 agcgtgctgg ctcagagcac agtaagcggg gttgtgactg ataaaggatc agctcaacca
     7321 atgccgggag taaatgtaat tattaaaggc accactaaag gtgctacaac tgattttgac
     7381 ggtaaatatt cgctgtcaaa tgtaaaaagt ggcgacgtca ttgaattctc ttatgtagga
     7441 tttaagccag tagatgtgac gtatacttca caaaaatcaa tcgatgtttc tttggaagaa
     7501 gaatcagcaa aactagaaga agtagttttg gttggttacg gaaccgttaa gaaaaaagat
     7561 gcaacaggtt ctgtagtgca agtgactaca aaagatttta acaaaggggc taacgtaacg
     7621 gcggaaaacc tattaaatgg tagagttgcc ggggtgacca ttaacactag tggacagcct
     7681 ggttcaggat ctgccattag aattcgtggt ggttcatcat taggagcttc taatgatccg
     7741 ctgatagtta ttgatggatt gccaattggt gaagtagccg gaggtgcaac ttccgtatta
     7801 gcttctatca atccaaatga catcgagagt tttaccgtat taaaagatgc ttctgcaacg
     7861 gctatttacg gttctcgtgc ctctaatggg gtaatcatta ttaccactaa aaaaggggga
     7921 aaagaattag cagttgaata taattttcaa tatggctcag gtgaagtagc aaataaagtg
     7981 gatgttttct ctgcggaaga ctattataaa atggtggagg caaacagacc acaattttta
     8041 aatagattag gcgtagatga tcctaacaat cccggtgaaa aaatttatta tgataccgat
     8101 tggcaagatg aaatctacag aagaactgat ttagttgaaa atacgttgac agtgaagggt
     8161 tcattattca aaaaaatccc aactcgttta tctgtaggta atacgtatca ggaaggtctt
     8221 cgtttaacaa gttcattcaa cagaaattca gctaatttag cgatgaaccc aaatttcttt
     8281 gaagatcatt taaaactaaa attaaatgca aacttgtcga atgaaaataa cagatttgca
     8341 gacggcgtag aaggtggtgc aattcgtttt gatcctacaa aaccggttta tgattcaaat
     8401 tcagcctatg gagggttctt ccaatataca gatgctaatg gacttgcttt aaatggtgta
     8461 aaccgtaacc cggtggccgc tttattacaa agaaagaaca ttacagaggt aaatcgtatt
     8521 tttggaaatt ttgaattgga ctacaaatta catttcttcg aagatatgag agcggttgta
     8581 aatgtaggat gggataaaag taaaggtagc ggttcaaatg agttatctaa agaaagtcct
     8641 gatggccaac gtaacggaaa tacaactgct attttaacca atggtagtta tactgaattt
     8701 accagtgaaa gagagaatac tttgttagat gcctatttag tttacagtaa atcattagaa
     8761 aattttagag ctgaagtaac aggtggttat tcttatcaaa aatttaccgg ttcaagtttc
     8821 agttcaaaag attacagaag atttaatgat gccggaaatc aaaataatta ctacacggaa
     8881 acagatgttg ttttaatcgg tttttttggt agagctaatt taacttttaa tgagaaatat
     8941 ttagttacct tatcatacag aagagacggt acttctagat tctctgaaga caatcgttgg
     9001 ggtaatttcc cggcagctgc tattgcttgg aaaataaaag atgaatcgtt cttgaaagat
     9061 tcaaaaactt tttctgattt aaaattaaga gcaggttggg gtataacagg tcaacaagat
     9121 ttaggagcaa gcagatttga ttatttatct actatgtcga caggtcagcc tggtaatgat
     9181 tatatgtttg gtggagaacc aatcaatcct ggatttccta attatacaaa ccctgatttg
     9241 aaatgggaag aaacaacaac ctataacgta ggtatcgatt acgggttctg gaataacaga
     9301 attacaggag ctttagactt tttctacaaa caatcagatg acttattagc gagagctcca
     9361 gtgccggacg gttcaaactt tagtaatgtt ggatcacaaa acattggtgc atttacttct
     9421 caaggggttg agttcgctat taatgccgct ctttatcaag gagatggttt taattgggat
     9481 atcaatttta atgccactca ttttaatact aatattgata aactagctgc cggtattgac
     9541 atcgaagttg gtggagttgg ttctggtaca ggaggaacta ttcaagttca ttctgaagga
     9601 tttacacctt attcttactt tgtttacaaa caactttatg atactgccgg tgcacctatc
     9661 gaaggagcat atgcagatat taatagagat ggtataatta ctgcggctga tagatatata
     9721 tataaaaatc ctgatccgga agcagtctta ggttttgctt ctaacatgag ttacaaaaat
     9781 tttgattttt catttaactt gagagcaagt attggtaacc atttatataa tgctgtgaat
     9841 gcaggtaacg ctcagtatga taacattgaa agtacgcagg tggttgcgaa tttaccaacg
     9901 gcggtgttag attctaattt caatactaca tcagacgttt tattttctga ttattatatt
     9961 gaaaatgctt ctttcttaaa aatggataac atcacgttag ggtatacttt ttcaaaaatc
    10021 tttaatgata aagctacttt acgtttaagt accggcatgc aaaatgtttt tgtaatcaca
    10081 gaatatagtg gtttagatcc tgaggttttt ggtggaattg acaacaccat ttacccaaga
    10141 ccaagaacaa tattatttgg tgccaacatt aaattttaaa aaaaactatg aaaacaaaaa
    10201 ttaaattatt aaccgcattt tcagtacttc ttttcttagg agcatgtact gatgatttga
    10261 atgttactcc gcaagatgac aatttgttcc ttacggatga cttctacaca acggatgcct
    10321 cttataaaca aggtttggcg ggggtttatt caaatttatc cttaaccgga acaagtggta
    10381 ctggttcttc tttcattgaa ggattagatg ctggaaccag ccaatatggt agagccttat
    10441 ggtatatgca agtattggcg gctgacgaag cagtttggtc atatgaaaat gaccctggtg
    10501 ttagagaaat tcaacgtaat atttggtctg ctgaaaatcc tattgtacgg ggtatgttcg
    10561 gtagagctat ggtacaggtt tcgtttgtaa atgaatattt gagacaaaca acagatgcta
    10621 aattagatgc tagaggtgtt ggtgaagcag ttagagctga aatacctact tttagagctg
    10681 aagcaaggtt gttaagagca ttagcttact atcacttgat ggatttatat ggaaaagctc
    10741 ctttcttaac agaagctgat ttacctggtt ttacagccgg tcctcaatat gatagagctc
    10801 agttgttcgc tttcttagag gaagaattaa cagcaattga agctgattta aaagctccga
    10861 gaactaacga acatggaaga gcagacaaag cggtggcttg gatgatttta gctaaaatgt
    10921 atttgaatgc tgaagtttac attggaacac caaaatatac agaatgtatt gctcaatgta
    10981 ataacatcat tggtggagga tattcattag caccaaatta tctttacaat ttcatgtctg
    11041 atagtgacac aaacagtgct aaaaatgaaa tcattttccc tttaatttct gatggtcaat
    11101 atactaaaaa ctacgggcct acaacagtta tgattaatgg tgaagtaggt tctttggaac
    11161 aaaatggtgt atctcttggt gtaggtgccg gaggatgggg cggagcctta cgtgttagaa
    11221 aacagtttgc aaacaaattt gaagatgctt tgtataatgg tgatgcacgt aatacattaa
    11281 ttaaagcaaa tcgttctgct gaaatcggtt ctatttctga tagagattct ggttttgtaa
    11341 ttactaaatt ctctaataga acgtctaacg gtggtcaagg ttctgacttg acttttgtgg
    11401 atacggattt cccattattc cgtttagcag acgtttatct aatgttagca gaagctcaaa
    11461 tgagaaatgc tggtgctacg aatagttctt tagatcaaac aggaatagca actcaacaat
    11521 cggttgatta tatcaatgat ttacgagaaa gagcctttgg taatgaatct gctaatgtgt
    11581 ctgtttcaca agtttcgctt caatttattt tagatgaaag attaagagaa ttacattggg
    11641 aatcacacag aagacaagac ttaattcgtt tcaacagata tactggagga aactacaatt
    11701 gggcttggaa aggaaatggt tctaatggaa tttcaatttc cggtaactta aaagtgtacc
    11761 ctcttccatc tcaaagttta gctgttaatc caaatttaac tcaaaaccca ggttattaat
    11821 catatcaatt tataaaatta tttaaaaatg aaaaatacat tcaaattttt attcgctttt
    11881 atcgcttttg ccggacttat gtcttgcgag gacgaagaaa acttccaaat ccttgatcca
    11941 tcaggggatt ttgtaatagt tacaccggat gctggaacaa acatcgtttt agatccaaat
    12001 ttacaaacaa atccaagtat tacattaact tggtctcctg ctgattatgg tacaccaacg
    12061 gaaattcaat atgtagttga atttgctgct gcaggtactg attttgctga accggttgtg
    12121 gctgcttcta ctacaaatac aaatgtaact tggaatgtgt tagaattaaa cggagctgca
    12181 ttatcatcag ggttgacacc ttttgaagaa ggaggactag atattagagt aaaagctact
    12241 acaggaacta ctggttctca agaaagaatt tctaatatgt taactatttt tgttactcca
    12301 ttcagtacag atttacctaa attatggttg ccaggaagtt atcagtctga ttcaggttat
    12361 ggaaataatt ggacacaaga aacagctgca actctagctg cttctgcata tggaaattac
    12421 gattttgaag gatatgttta ttttgcctca gctcaatcag ctcctgataa tggatataaa
    12481 tttacagatg ctccaaattg ggacaatgga gtttttggag acgatggttc tttttcaggt
    12541 gctttaacat ctccagggga taatatttct gcaaatcctg gatattattt agttaaagca
    12601 aatcctagta gtgcttctgc gggtggtttg gctgcaatgt cttatacagt tactgcaaca
    12661 gaatggggaa tcgttggagc tgcaacacca aatggttggg attcgagcac tcaacttacc
    12721 tataattcat caacaaaaat gtgggaaggt gtcgtggctt taactgcagg tgaattcaaa
    12781 tttagagcta accaagcttg gactatcaat ttaggtggtg acccagctgc aatgaactat
    12841 gatggaggta atttatctgt tgctgccgaa ggtacttatt tagttaaact taatttaagt
    12901 aatcctagag catatacgta tgaattaatt gctcaataat ttcttttaaa ataataaaaa
    12961 gggctgtaat cctacagccc ttttttatca tttaaatttt cagctatgaa aaaatattat
    13021 ttttttactt ttttattttc tttattttct gggttagttt ttgctcaagt aacgattact
    13081 ccttccacgt ttaatgtaac ggaccaaatt accattacag tatcaacagc cgcccaagcc
    13141 tgtaacctta tggggacaag tcctgcaaaa gtttatatgc atgccggtat tggcgatgat
    13201 acaaatgctt ttggttattc tgttattgga acttggggac aagataatgg cgttgggcta
    13261 atgacaaaca atggaaatgg aacgtggtct attactatta caccaagtgt ttattttgga
    13321 ttaaatggaa cacaacaagc caatgctaca aaacttggaa tggtttttag aaatgcgaat
    13381 ggttctcaaa cgttaaaatt accaccatca tgcggtgatt ttattttcaa tgtaggaact
    13441 tttcagttaa acttgacttc tccggtcaac aacagttcaa caatcatcaa tagcggaggt
    13501 agtttggtag tatctgctac aaatacaggt ggtaatgcca gttatgtttt gaaagcaaat
    13561 ggaaatccta tcaattcaaa tggagctact gcaaattatt ccttcactca ttcaactatt
    13621 acttcaaatc aaaattatga gttggtcgta acacaaggaa gtagtacaat tactagaaca
    13681 tttaaagtaa ttgtaaatcc ggggactatt tcccaggcaa ttcctgcagg gatggaagat
    13741 ggtatcaatt ataatgcatc tgatccaaca aaagcaacac tgaagttaaa tgctccgggg
    13801 aaagattttg tttatgtggc aggaagtttc aannnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnagagtagc tgttttaaaa gaatatgctg attattcttg gtcattagac
    13981 ccgaatcatt atgtgatttt tgaacattta ggggcggata atgaagaaca acaatgggca
    14041 aattatagaa ttaatgaagg aaaaggggtt atgatgtggg gtgaactttg gaatccttac
    14101 aaacaactta tcagtggaaa ttcaacaaat gctgatataa caagaatggg gcatgtcagt
    14161 agaggtttta ccggaaaaag attaatgggt tatgccgaaa gtcatgataa agacagaatt
    14221 atttatgaag cagtaacttt tggaaatggt ggaggaaccg ctcctgttag tggaaactta
    14281 aacaatacaa ttccaagaat gaaggcgatt ggtgctacca gtattttaat tcctggtcct
    14341 aaaatgattt ggcattttca agaattaggg atggacgatt ctatttttac ttgctcagga
    14401 ggcagttccg taaatacaga ttacgatgct attccaggag attgtaaatt agacacgaag
    14461 cctcagcccc aatgggttga aaattggtta ggcgatgcac ttcgcaattc agtttacaat
    14521 gcttatgcaa aatttattag tttaaagaaa aatgaaccgg tatttaatgg tgaatatgct
    14581 attagtccaa atggcagtaa tattcgtcaa cgaatttatg tgttcaataa ttcattgcca
    14641 tctacacagt taaaaaatgt agttatttta gcaaactttt ctgtggccag tcaaaatatc
    14701 acacccgatt tcccatatat aggaacttgg tatgatttaa tgacggatac cccaataagc
    14761 gtaaccagca caactgcaca gattgcatta gccccgggag agtttaaagt ttatggaaac
    14821 agattgccaa cgcttagtgc cgaaactttt gaattgaaag atgatattgc tattgcacct
    14881 aatcctacta ccgagacatt ttctgtgaca gttccaacgg taaaggttga aatttattca
    14941 attacgggtc aattggttaa aacatttcaa aatgattttg atacaaacta tcgttttgat
    15001 gtaagtgatt taacgaaagg aatttatttt gtgaaagtaa aagatcaaaa tgaccgtgaa
    15061 aaatcaatga aattgcttaa aaattaaaat ttgagttagt taatatgatt ataatgccat
    15121 taaacccagc tttttagttg ggttttttat tttaatattt tgctaattaa tagattatga
    15181 atactttcgt agtatgaata aagcaggtaa aaaacgcata gttaaaatag ttaaaatagc
    15241 tggaatatct cttggaacac tactgttatt gctttttttg ttgccaacaa ttttccctga
    15301 gacattaaca aatcaagtaa aaaagtttgc caatgagaag cttaatggag aattaagttt
    15361 caaagaaact cacctgtcct tttttactca ttttccttcg ttaaccttat cattggatga
    15421 ttttttatta aaaggatctg caccatttca aaaagacacg cttattgctg caaaggaagt
    15481 ggcatttggc ataaatttaa agtctgtttt ttttgatgaa aaaattaaaa tcgataaaat
    15541 attcatttca gaaggcacta ttttaatcaa agtaaacaaa aaaggagagc caaactacaa
    15601 tgtttatgtt tcagaggaag cagcaactgt ttcagattca acctctgcat ctttaaagtt
    15661 ggaacgaata gatgtcaaaa atactaggtt agtttatgat gatcaatcta ctaaaatttt
    15721 aattaacgca aaaggcttta attacctggg taaaggtgat ttggataaag cagtttttga
    15781 tttgaaaaca aaagcacaaa ttgagtcgtt tgatttcagt tttgaaaacg aacaatattt
    15841 aaaaaacaaa aaagttaatg cagaattaat tacaaaaata aacaccaatt ctcttgctct
    15901 agtttttgag caaaataact taaaaatcaa taaattgccg gttgaattta aaggaaaatt
    15961 caacttttta aaaaatggat atgatttaga ttttagtgtg gaatctcaga atagtcagtt
    16021 aaatgacttc tttacagctt taccaccaag ttatattaaa tggttagata agacggaaat
    16081 taagggcaag acagatttat tctttgagtt gaaaggacaa tacattgcct ctgaaaataa
    16141 aatgccaaac gttcatttca atatgaaaat cagagatggt tatatctccc atcaaaaaac
    16201 cccttttccg gccgaaaata tatttttgaa ttttgatact aaattacctt ctttagaccc
    16261 agaacaattg gcagtaaaaa ttgactccat ttattttgat gttggggaag atttttttaa
    16321 tggaaatgta attgttaacg gactttcaaa accgaaaatt gatgcccgaa ttcgttctaa
    16381 aatggattta gcaaaattgg ataaagcttt gggtattcaa aatatggatt taaaaggcac
    16441 tttcacagca gatattgtcg caaagggaat ttatgataaa gaaaaaaatc aatttccgat
    16501 aaccaaagga gatttagagc ttaaaaatgg acatattaaa tcaacctatt atcctaaccc
    16561 tattgagaac ataaatttta aagcaaatgc atcaaatcaa acggggcaat taaaagattt
    16621 atctcttgtc atcaatccgg catcactcca atttgaagga aaaccaattg caatgaatgc
    16681 atcatttcaa aattttgaag atatttcgta tgatataaaa gcgaaaggtg aattagatat
    16741 tgaaaaaata tataaagtat tttcacaaaa agggttagat gtatccggat atgcaaaaat
    16801 ggatgtctca tttaaaggaa aacaaagtga cgctactaat ggcaactaca ataaactaga
    16861 aaataaaggc attctttatc tgagagatat aaaaacaagt tctgactatt tgcccaaacc
    16921 gtttatcatt aaagagggcg aatttgtttt taatcaagat aaaatgaatt tcaaaaactt
    16981 taaggcaaac tatgggcaat ctgatttcat gatgaatggt tacatggaaa atgtgataaa
    17041 ttttgccttg tctgattctg aaattttaaa aggaacattt gcagtagaat ctaatttgat
    17101 tcaaataaat gagttcatgg cattttctga taattcaaaa gaattgcctg aatcagccga
    17161 aagtgaacct gtttctcaaa gtggagtgat agttgttccg actcaattta attttaattt
    17221 acttgcaaag gccaataaag ttgtttttga tgagatgaat attgaaaatc tttccggaaa
    17281 aatggttgtg aatcaaggga aattggctct tcaaaacact tctttttcac taattggtat
    17341 aaaagtaact atgagtgctt tatatttcaa tgaatctaca aacaaagcag attttgattt
    17401 taaaattaaa gcaactgaat ttaatattaa gagagcttac aatgaaatac ctatgtttcg
    17461 tgaaatggct tctgctgctg aatatgcaga aggtattgta tccttagatt acaaaatagc
    17521 cggaaaaata gatcaaaata tgtccccaat ctatccttct cttgttggtg gaggaacact
    17581 ttctgtcaag aatgtaaaaa tgaaaggatt caaattgttt aatgcagtaa gcaaaaaaac
    17641 agaaacagat gcattaaaag accccgatgt ttcaaaagta gatattaaaa cgactgtaaa
    17701 aaataatttg attaaaatag aacgattcaa gtttaaggtg gcaggattta ggccaagaat
    17761 tgaaggagaa tcaagttttg atggtaaatt aaatataaaa atgaggctgg gcttacctcc
    17821 tttaggaata ataggtatcc caataaacgt gaccggaact caagaaaatc ctattattgg
    17881 agttggaaaa cagacggaaa acttagaaga aactgagtac gaggacggaa cttctcctca
    17941 agtcgttcct gaacagctgc ccgaaacaat caaggacact attaaataac aaaaaacccg
    18001 aatattgcta ttcgggttta agtggtacct ccag
//
LOCUS       DAOTCW010000040        17852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1751_length_17852_cov_42.200202, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000040 DAOTCW010000000
VERSION     DAOTCW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 17852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17852
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1751_length_17852_cov_42.200202"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="P5335_04965"
     CDS             <1..1362
                     /locus_tag="P5335_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016987997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRZ74264.1"
                     /translation="SSLQVKDFIWKGLNLYYLWQADEPNLADNRFAMQSDLNFFLEGY
                     ETPELLFEDLLVARTTDRFSVIFNDYTVLEGLLQGTAKSNGVDYGLRYKTGSTTEIYG
                     WVRYILPNSDASAKNIQRGDIFYSVNGTPLTSNNYRDLLNLESYTLNLADYNAGNITP
                     NGESVELSKTAYTENPVFQINVVEIENKKIGYLMYNGFYSGFENQLNQAFGQLKSQGI
                     THLVLDLRYNSGGSVDTATRLASMITGQFNAEIFAKQQWNAKVEVYYNDTNPSALVNR
                     FTDVINNGNLINSLNLTKVYIISSGSTASASELVINCLKPYINVVQIGTTTVGKNVGS
                     VTLYDSPTFGKEDKNPNHKYAMQPIVLRITDKNGFGDYVNGLEPTIEQAENLANLGVI
                     GDQNEPLFNTAINYILDNGRMGQPYTEDKQVYFKDSKSIRRFGNEMYLEELPEGFNSF
                     LLK"
     gene            complement(1403..2467)
                     /locus_tag="P5335_04970"
     CDS             complement(1403..2467)
                     /locus_tag="P5335_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008272002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74265.1"
                     /translation="MNLKKIGILLTLSGLFLTACTNDDDVSIPLGAYDNGVLILNQGN
                     FGGGNSSISFWSNDFSTFEFNAFSSVNPSVTLGDTAQDLGFYGDKAYIVVNLSNTIQV
                     VNRYTLEHLTTISTGLSNPRYIAFANGNAYVTNWGDASNAEDDYVAVINLTSNSVTSF
                     IPVTEGPERILEHDDKLFIAHMGGYGFGNSISVIQANNNSVSSIEVGDVPNSMALVNN
                     DLYVLCGGKPSWAEPETSGSLVKINLSTNTVTAALPFLLGNHPSNLIFHDNKFYYTVD
                     SNVFSKTIEANTIPSTPIFSTTAQGVYGVYSFAIANNKIFVGDATDYNSNGKVYIYSL
                     TGTLENQETVGVIPGGFYFN"
     gene            complement(2470..4299)
                     /locus_tag="P5335_04975"
     CDS             complement(2470..4299)
                     /locus_tag="P5335_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ74266.1"
                     /translation="MTWKNLYCCLAFLLYSFSWGQNDSIIRLNEVIVSDSQLKNFSKS
                     QTVTILNDSILNKNQGSLTSLLSTNSTIYFKENGAGMVSSASFRGTTAQQTAVIWNGI
                     NINSQLNGQTDFNTINPTDFTNISIRPGGGSVIYGSGAIGGSIHLNSELKFNTATSHL
                     FRLNYGSFETYGSNYQLEIGKEKWAVQASISANSSKNDYEYIGYDKKNENGEFTNQSY
                     NFSVGHKINDTNFIKFYSFLFNGERHFSGTITVNSDDKYLDFNTRNMLEWILFKNKYT
                     SKVKAAYITEEYKYYENRNSDNHTFGQVNSLFLRHDFIYSISESITINTLLDFTQSDA
                     KGSDLLTETRTIGTGSILWKHKLSTKLNYELGIRQEVTDTYESPLLFSAGTLFEVNGF
                     YKIKANLSKNFRIPTFNDLYWQGSGNTDLNPEHSLQGEIGNELNYKNSKFVLTGFIIQ
                     LKDMIRWIPTSNGLWQPENTQSVSNYGLEIQFETKKSIDKHLFSFTSGYGYTVSVNNQ
                     TEKQLTYVPNHKANTNLAYNYKSFTTYYQWVYTGEVFTSSDNFYKLDDYALSNIGIDY
                     NFSKKKTYLLGFQVLNLTNERYQNVISRPMPGRNFKLYLTLNL"
     regulatory      complement(4330..4591)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4825..5955
                     /locus_tag="P5335_04980"
     CDS             4825..5955
                     /locus_tag="P5335_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRZ74267.1"
                     /translation="MKIRLLALFFMLCLFACQSTKEKETVIISSENSIRYATGLSIQK
                     FNQYSVVTVSNPWPTAEKNYTYILHQNGVILPDSLKKFTKIQVPLQSIVVTSTTHIPA
                     LELLGVEKSLIGFPNTDYVSSPKTRALIDAKKVREIGTNQSLNTEVLLDMHPDVIIGF
                     GVDGEKKTYDQLQQNGLKILYNGDWTEHHPLGRAEWIQLFGVLFGLEEKSDLVFRKIE
                     KDYLDAVDLAKKADSKPTILSGAIYQDQWYLPQGKSWAAQFLENAQGDYLWNDSKGTG
                     SLSLSFETVLEKAQNADFWIGPGQFTSFEQLEKANPNYSYFKAVQNKNVYSFSSKKGK
                     TGGIIYYELASSRPDLVLKDLIKILHPKLLPEYELYFFEKLK"
     gene            6095..7051
                     /locus_tag="P5335_04985"
     CDS             6095..7051
                     /locus_tag="P5335_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRZ74268.1"
                     /translation="MFLLNLSLGSVSIPTDAILDSVVGNEIKNSWEIILWNFRLPKAI
                     TAILVGMGLSISGLLMQTLFRNPLAGPYVLGLSSGSSLGVAFVIMGAGFLPAFLVSDY
                     GIVIASSLGSFLVLMAVLVVSQKLRDTMAILIVGLMFGSFTSAIVSVLSYFSTAQELQ
                     KFTFWSMGSLANLSWNSVLILAICCSFGLLLSILSIKPLNALLLGENYAKSLGLNFKR
                     TRFIIIFATSILAGSITAFAGPIAFVGLAVPHLAKLLFQTSNHFILFWSTLLLGAILM
                     LVCDSIAQLPGSDYTLPINAITSMVGAPVVVWLLVRKRKIMI"
     gene            7077..7490
                     /locus_tag="P5335_04990"
     CDS             7077..7490
                     /locus_tag="P5335_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRZ74269.1"
                     /translation="MITQKYLDELTYEVVGSAIEVHKIMGRGLFESVYHQCLIEELKH
                     RKIYFLTEMKVPVIYKGKSLDINFRCDLFIENCLVVEIKSVIDLHKITEAQLLNYMNL
                     LKAPKGILINFNCRNIFNEGQKTYVNEYFKKLPQY"
     gene            7588..8358
                     /locus_tag="P5335_04995"
     CDS             7588..8358
                     /locus_tag="P5335_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRZ74270.1"
                     /translation="MILQTQHLSIGYSSKAQTKVIASDVSIQLEQGKLIGLVGANGIG
                     KSTLLRTLTGIQKSLSGTVLLNEKPIDSFEPLALAQQISVVLTEKLPPSNLTVYELIA
                     LGRQPYTNWLGKLAEEDKIQIENAISLTEIGHLIHQKYYEISDGQLQKVLIARALAQD
                     TPLIVLDEPATHLDLLHKVSLFKLLKKLTVETQKCIMFSTHDIDLAIQLSDEMIVMTN
                     EKVIQNQPCNLILDGTFSNLFLSENILFDSEKGKFIIR"
     gene            8425..9789
                     /gene="rmuC"
                     /locus_tag="P5335_05000"
     CDS             8425..9789
                     /gene="rmuC"
                     /locus_tag="P5335_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016987987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRZ74271.1"
                     /translation="MTTSFLLLLTFVLALSIGIFIGKMLSTAQSKSEKSALEERINGL
                     LIQLDQSKNRFQEEQNQFVKQLESEKKEKEEIRREKDSLSLQLTKKEADFENLWQRNK
                     EQKQEVEELQEKFTKEFENLANKILEEKTVKFTEQNKENLKNILSPLQEKIQHFEKKV
                     EDTHKESIDYHAALRQQIVGLKEMNEQMSKETLNLTKALKGDSKIQGNWGELILERVL
                     EKSGLEKGREYEVQQSFTNEEGNRVFPDVVINLPDGKKMIVDSKVSLTAYERYTNEED
                     DLQKSSFLKEHINSIKRHVEQLSDKNYHDLYQMESPDFVLLFIPIESAFAIALNEDNS
                     LYNKAFERNIIIVTPTTLLATLRTIDSMWTNQKQQENALEIARQAGALYDKFEGFVTD
                     LVKIGKKMDEAKVEYGNAMGKLVEGKGNLVGSVERLRKMGAKAKKVLPENILKRAGEE
                     LEEE"
     gene            9982..11409
                     /locus_tag="P5335_05005"
     CDS             9982..11409
                     /locus_tag="P5335_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74272.1"
                     /translation="MKKNYAYLLPLFIAIASLFIAVSCSNDDDYVPIVDVLPVATDDT
                     ATYALAETVEIPVLLNDTTGDEVIPTTVSLVGGIDTDSNTTLDELTIPGEGVWTVNAL
                     TGVVTFTPNSSFQNNPTPIKYTVEDLEENISNQATITITAVQTADVDLTQVPYQKLSD
                     YKFFAGEMKNQIPSLGVLPYEPASPLFTDYAHKKRFVWMPNGVKATYEGDGNLLELPV
                     GSVIIKNFYYDNVQPGNVTRIIETRMMIRKAEEWIFAEYVWNTEQTEAFLDMNGSNTS
                     ITWIDENNVTKSTSSYRIPSGPQCMTCHKSNEVAVPIGIKPQNINFNYTYSTGSKNQL
                     SKWIEQGYLENDLPGNIVSTVDYRDTSKPLELRVRSYVDMNCAHCHRNNSHCDYRPMR
                     FAFGETTNLVNMGVCVDTQDMAGFPSALSKIVTPGNSNRSMLHYRLNTADPAYMMPLI
                     GRSMIHEEGAALIQEWINSLSGPCL"
     gene            11447..13501
                     /locus_tag="P5335_05010"
     CDS             11447..13501
                     /locus_tag="P5335_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRZ74273.1"
                     /translation="MKKITYLILFCIGIQLSNAQDTCASAAVLPGAGLYIVDAVNGLE
                     VPNPVCTENGNIANNGKGEWYSYTPTQNYTITITTDIAANTPRVDTRFHVYTGTCGAL
                     TCYAGDDDEGIGDTNYSSIDTFVVLANTTYTIAFDNRWTANGFTFQLIEAPYQEPIIN
                     PVTFTNQAIASVNSQYNITVVDMNGDYLDDIVGVNQDNIRIHTQNAGGTFTVTDYPTT
                     SATNMPSWSLAAADYNKDGLNDLMYGGGNGVTFMKSNEAGNGYDQNTFSEYVFCQRTN
                     FVDINNDGHLDAFSCHDVQPNVYFLNDGWGGFTFYQSTVTPGAILLGIHPNGGNYGSI
                     WIDYDNDGDQDLFIAKCRGGSGTAKFNELHRNNGDGTFTDVSTAANMYDPVQTWSSAW
                     ADYDNDGDMDALIGASSTTDGTHKYMRNNGDGTFTDITAGSGWDINASTNIEHIAHDF
                     DNDGFVDVMGGGNKIMYNKGDGTFAPSGVGFGVSPVGDLNNDGFIDFQTGATLRINNG
                     NSNKWIKLNLQGIQSNRNGIGARVEIYGAWGKQIRDVRSGEGFKYMSSLNVHFGIGQA
                     TAIDQVIIRWPSGLVDTIVNPSPNQTLLVVEGATLSVNQNTISNFVLYPNPAKDFVTL
                     KSSTTSSIVSVEIFDLNGRKVLTSDVVKETISVQSLSVGTYLLLAKDSDGNLSTQKFI
                     KQ"
     gene            13591..>14762
                     /locus_tag="P5335_05015"
     CDS             13591..>14762
                     /locus_tag="P5335_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HRZ74274.1"
                     /translation="MNYILFDGPVRNALLPFTFTRPVADIRIGILTIREKWEKYLGYT
                     TTTITEEYLSEKYPMVEMEENILINASFLPNEVLVEMIKSLEKNQAIFKGEEVVAFFT
                     NNTQEEVDFDTYEIIDYNEEGLRIEHTWDIFAKNDAALREDFELLTEGRTSQPIPKSV
                     NVISPENIFIEKGAKLEFVTLNGSTGPIYIGYNSEIMEGSVIRGPFALCEEAQVKLAT
                     KVYGATTVGPHCRIGGEVNNSVLFGYSNKGHDGFLGNAVLGEWCNIGADSNNSNLKNN
                     YDEVKLWSYETEGFEKTGLQFCGLMMGDHSKCGINTMFNTGTVVGVSTNIFGSGFPRN
                     FVPSFSWGGAAGFSTYITKKAFETARIVMSRRHVEFTEQDARILEHVFEETKKWRK"
     assembly_gap    14763..14862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15004..16605)
                     /locus_tag="P5335_05020"
     CDS             complement(15004..16605)
                     /locus_tag="P5335_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRZ74275.1"
                     /translation="MKKILFLVTLSLQLLLLSCHDSKENNKELLVFRYNESANIATLD
                     PAFSKSQNIIWACNQLFNGLVQLDDSLHIQPDIAKSWTISEDGKTYQFVLRKDIYFHK
                     HILFGKDSTRTVKASDFEYSLKRLKDEKVASPGGWILQNVEKIQALNDTLVEIKLTQS
                     FPAFLGLLSMKYASVVPKEIIESSNYDFRTKPIGTGPFQFKFWEENVKLVLRRNPIYF
                     EKDEKGIQLPYLEAVAITFLPDKQSGFLEFIQGKIDFISGLDPSYKDEILTQKGELQP
                     KYADDVNLISGPYLNTEYLGFRMDGTDKTILDKRIRQALNYGFDRAKMITYLRNNMGI
                     PATSGVIPSGMPSFNNQKGYDFEPEKAKSLVAKFVKETGVKKPKIFLSTNASYIDIGE
                     FLQREWQKIGLDVEIDVNPPSTLRQAIATGKVTFFRASWIADYPDAENYLSLFYSKNF
                     APNGPNYTHFSNAKFDALYEKAFLETDDVKRFEFYQQMDKIIIEEAPIIPLFYDKVAR
                     FTRKNVEGLGINPLNLLSLKRVKKN"
     gene            16739..>17852
                     /gene="mtaB"
                     /locus_tag="P5335_05025"
     CDS             16739..>17852
                     /gene="mtaB"
                     /locus_tag="P5335_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006261841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRZ74276.1"
                     /translation="MEHKKKVAFYTLGCKLNFSETSTIARNFQHEGFERVDFEEVADI
                     YVINTCSVTENADKQFKQIVKKALKNNNKAFVAAVGCYAQLKPEELAAVDGVDLVLGA
                     TEKFKITDYLNDLSKNDLGEVHSCEIEEANFYVGSYSIGDRTRAFLKVQDGCDYKCTY
                     CTIPLARGISRSDELQNVLKNAKEISQQGIKEIVLTGVNIGDYGKGEFGNKKHEHTFL
                     ELVQALDEVEGIERLRISSIEPNLLKNETIDFVSKSRTFVPHFHIPLQSGSNDILKKM
                     KRRYLRELYVDRVNKIREVMSDACIGVDVIVGFPGETEEHFLETYHFLNELNISYLHV
                     FTYSERDNTEAAEMEGIVPGNVRSKRSKMLRGLSVKK"
BASE COUNT         5808 a         2802 c         3322 g         5820 t
ORIGIN      
        1 tcttctttgc aagtgaaaga ttttatatgg aaaggactca atttatatta tttatggcaa
       61 gccgatgagc caaatttagc cgataatcgt tttgcaatgc aaagtgattt aaattttttt
      121 ttggaaggat atgaaactcc tgaattgctg tttgaagatt tattagtagc cagaactacc
      181 gatcggttta gtgtgatttt taatgattat acagttttag aaggactttt gcaaggaact
      241 gcaaaaagta atggggttga ttatggtttg cgatacaaaa ctgggagtac gacagaaatt
      301 tatggatggg tacggtatat tttacccaat tccgatgctt ctgctaaaaa tatacaacgc
      361 ggtgatattt tttattctgt taatggaacg cctttaacga gtaataatta tcgtgattta
      421 ctgaatttag agtcttatac gctaaattta gccgattata atgccggaaa tatcacgcct
      481 aatggagaat ccgttgaact ttcaaaaact gcttatactg agaatcctgt ttttcaaatc
      541 aatgtggtgg aaattgagaa caaaaaaatt ggctatttaa tgtataacgg tttttattcc
      601 ggttttgaaa atcaattgaa tcaggctttt ggtcagttga aatcacaagg catcacccat
      661 ttagtgcttg atttacgcta taattccgga ggctcagtcg atacagcaac tcgattagcg
      721 agtatgataa ccggacagtt taacgctgaa atttttgcca aacaacaatg gaatgccaaa
      781 gtggaagtct attataatga caccaatcct agtgctttgg tgaatcgttt tacagatgtt
      841 attaataacg gtaatttaat caatagttta aatttaacca aagtttacat tatcagttca
      901 gggagtactg cttctgccag tgaattagtt attaattgtt taaagccgta tatcaatgta
      961 gttcaaatcg gaacaacaac agttggaaaa aacgttggtt ctgttacatt gtatgactcg
     1021 ccaacctttg ggaaagaaga taaaaatccc aatcataaat atgctatgca acctattgta
     1081 ttgagaataa ccgataaaaa cggttttggt gattatgtta atgggttaga gcctacaatt
     1141 gaacaagcag agaatttagc taatttaggg gtgataggtg atcaaaatga accacttttc
     1201 aatacagcca ttaattacat tttagataac ggacgtatgg ggcaaccata tactgaagac
     1261 aaacaagtgt attttaagga tagcaaatcg attagaagat ttggcaacga aatgtatttg
     1321 gaagaattac cagaaggatt taattccttt ttactcaaat aaaaaagagg atgttcattt
     1381 gaacatcctc tttttgtagg tattaattaa agtaaaatcc tcccggaatg acacctactg
     1441 tttcttggtt ttccaacgtt ccggttaaag aataaatgta tacttttccg tttgagttat
     1501 aatcggtagc atcgccaacg aatattttgt tatttgcgat tgcaaaactg taaacaccat
     1561 aaactccttg tgctgtggtg ctaaaaattg gagtagaagg aattgtattt gcttcaattg
     1621 tttttgaaaa cacatttgaa tctaccgtat aataaaattt attatcatga aaaattaaat
     1681 ttgaaggatg atttccaagt aaaaaaggta aagcagcagt aacagtattt gtggaaagat
     1741 ttattttaac aagtgaaccg gaggtttcag gttcagccca agaaggtttt ccgccacaaa
     1801 gcacatataa gtcattgttt acaagtgcca ttgagtttgg tacatcccca acttctatag
     1861 atgatacgct attattattc gcttgaataa ccgaaatgga atttccaaag ccataaccac
     1921 ccatgtgtgc tataaataat ttgtcatcat gttctaaaat tcgttccgga ccttcagtca
     1981 ccggaataaa ggatgttacg ctgtttgatg ttaagttaat taccgccaca taatcgtctt
     2041 ctgcgttact tgcatctccc cagttcgtta cataagcatt tccatttgca aaggctatat
     2101 atctaggatt ggataatccg gtagaaattg tggtcaaatg ttctaaagta tatcggttta
     2161 ctacttgaat ggtatttgag aggtttacaa ctatgtaagc cttgtctcca tagaaaccta
     2221 aatcttgtgc ggtatcaccc aaagtaacag atggatttac tgaactaaaa gcattaaatt
     2281 cgaaagtcga aaaatcattt gaccaaaagg aaatggaact gtttcctcct ccaaaattcc
     2341 cttgatttaa gataagtaca ccattgtcat aagctcctaa tggtattgaa acatcatcat
     2401 catttgtaca tgctgttaag aataatccac taagagttaa tagaattcca atttttttta
     2461 agttcatgat tataaattta aggttagata aagtttaaaa tttcttccgg gcatgggtcg
     2521 ggaaattaca ttttgatacc tttcattcgt aagattcaat acttgaaagc ccaataaata
     2581 agtctttttt ttgctaaaat tgtaatcgat tcctatgttt gaaagtgcat aatcgtcaag
     2641 tttgtaaaag ttgtcagatg aggtaaatac ttcaccggta tacacccatt ggtaataggt
     2701 tgtaaaactt ttataattgt aggccagatt agtatttgct ttgtggttcg gtacgtaggt
     2761 aagttgtttt tcagtttgat tgtttacaga aacggtgtaa ccatagccgg atgtgaatga
     2821 aaacaaatgc ttgtcaatgg attttttagt ttcaaattgt atttctaaac cataattaga
     2881 gacgctttgc gtattttccg gttgccatag tccgtttgaa gtgggaatcc atcgaatcat
     2941 gtcttttaat tggattataa aacccgtgag cacaaacttt gaatttttat aattcaattc
     3001 attgcctatt tcaccttgaa gcgaatgttc aggatttaaa tcggtatttc cactaccttg
     3061 ccaatacaaa tcattgaaag tgggaatgcg gaagtttttg gaaagattcg cttttatttt
     3121 ataaaatccg tttacctcaa atagggttcc tgccgagaat aaaagcggac tctcataagt
     3181 gtcggtcact tcttgacgaa tgcccaattc ataattcaat ttagtcgaaa gtttgtgttt
     3241 ccaaaggata cttccggttc caattgttcg ggtttcggta agtaaatcac ttccttttgc
     3301 atcactttgc gtaaaatcta aaagtgtatt gattgtaatg gattccgaaa tagaatagat
     3361 gaaatcatgt cttaaaaaca gactatttac ttgtccaaaa gtatgattgt cagaatttct
     3421 attttcataa tacttatatt cttcagtgat ataagcagct tttacttttg aggtgtactt
     3481 gttcttgaaa agtatccatt ccaacatatt tcgggtattg aaatctaagt atttgtcatc
     3541 cgaattcacc gttattgtgc ctgaaaaatg gcgttcacca ttaaataaaa aactgtaaaa
     3601 tttaataaaa tttgtatcgt ttattttatg tccgacactg aaattataac tctgattggt
     3661 aaattcacca ttttcatttt ttttatcgta accaatgtat tcatagtcgt ttttggaact
     3721 atttgccgaa atactggctt gaactgccca tttttctttt ccaatttcta attgataatt
     3781 actaccatac gtttcaaaac ttccgtaatt cagtcggaaa aggtgagagg ttgccgtatt
     3841 aaactttaat tcggaattca aatgaatact tccgccaatc gcaccactgc cgtaaatgac
     3901 acttccacca ccgggtctga tgctaatgtt tgtaaaatcg gttggattaa tggtgttgaa
     3961 atcggtttga ccgtttaatt gcgaattgat attgatgcca ttccaaatca cagccgtttg
     4021 ttgagccgtg gttcctctaa acgaggctga agaaaccatt ccggctccat tttctttaaa
     4081 ataaattgtt gagtttgttg ataaaagtga tgtgagtgaa ccttgatttt tgtttaaaat
     4141 actgtcgttt aaaatagtta cagtttgtga ttttgaaaag ttttttaatt gagaatcgga
     4201 gacaattact tcgtttaatc gaataatcga atcattctga ccccaactaa atgagtacag
     4261 taaaaacgcg agacaacaat atagattttt ccaagtcata attttcaaaa cctttcttcc
     4321 cgaaggtttg atagttgtta tgtacttggc aggtctcctg gcttgcgtct tgttgttgaa
     4381 ccttcccgtt cgccgcggcg aacagtggta taaaaagtgt aacaacaagc ttgatagctc
     4441 acagttgcgg gtacagctca ggattttaga agtttttgga gttagtttac tgaatactga
     4501 aaactatcta ttgaacactt tttttcacct gattcccttt taatgtcctg ctgaaaaaat
     4561 caacagtgca acctaaatac gagtgtaaaa gtagtagttt tttggtttcg aaatctaaaa
     4621 acagattatt tttctttctt gtttgacatt tttataacca accaaatgaa tccaacaata
     4681 aagtgtagta ggatgattcc gccaacgatg tacatgaatg tattagaatc tctcatgatt
     4741 tatttttcgc caaatttaga atggaattct agtattcact ataacatttg ttacaaagaa
     4801 ctacatttgt aaaaaaatta tctgatgaaa attcgtttgc ttgcactttt ttttatgctc
     4861 tgtttatttg cttgtcaaag cactaaagaa aaggaaactg ttattatttc ttctgaaaac
     4921 agtattcgat atgccaccgg actttccatt caaaaattta atcagtattc ggttgtaact
     4981 gtttctaatc catggcctac cgctgagaaa aactatactt atattttaca tcaaaatgga
     5041 gttatactcc cggatagttt aaaaaagttt acaaaaattc aagttccttt acaatcaatt
     5101 gtcgtaacct ctactactca tataccagct ttggaattgt taggagttga aaaatctctg
     5161 ataggttttc caaataccga ttatgtttct tccccaaaaa ccagagcttt aattgatgca
     5221 aaaaaagtac gtgaaattgg aacgaaccaa agtctaaata ccgaagtttt attggatatg
     5281 catcctgatg ttatcattgg ttttggtgtt gatggtgaaa agaaaaccta tgatcaatta
     5341 caacaaaacg ggttaaaaat tttatacaat ggcgattgga ccgaacatca tccgttgggt
     5401 cgagccgaat ggattcaact ttttggcgtt ttattcggac tcgaagaaaa atcagattta
     5461 gttttcagaa aaattgaaaa agattatttg gatgcagtag atttggcaaa aaaagccgat
     5521 tcaaaaccaa ctattttaag tggagccatt taccaagacc agtggtattt gccacagggg
     5581 aaaagttggg cggctcaatt tttggaaaat gctcaagggg attatctgtg gaatgattct
     5641 aaaggaaccg gaagtttgtc tttgtcattt gaaacggttt tagaaaaagc tcaaaatgct
     5701 gatttttgga ttggtccggg acagtttact tcttttgagc agcttgaaaa agccaatccg
     5761 aattacagct attttaaggc tgttcaaaat aaaaatgtat attcttttag ttctaaaaaa
     5821 ggtaaaaccg gtggaatcat ttattacgag ttagcttcaa gtcgacccga tttagtgttg
     5881 aaagatttaa taaaaatcct gcatccaaaa ttattaccgg agtatgaatt gtactttttt
     5941 gagaagttga aatagtatat ttatcacatt tttaattata gtcttactat atttacgcct
     6001 cacttaatgt cccttaattc cttaatggtt gaaatcagtg ttttctcaaa aaaataaaat
     6061 actcttcttg tttttaagtt tcgctttaat cggaatgttt ctcttgaacc ttagtttggg
     6121 ttcggtttct attccaaccg atgcgatttt ggattcagta gttggcaatg aaatcaaaaa
     6181 ttcttgggaa attattcttt ggaattttcg tttgccgaaa gccataacag ctattttagt
     6241 gggaatggga ttatctattt ccggtttatt gatgcaaaca ttgttcagaa atcctttagc
     6301 aggtccgtat gtgttgggat taagttcggg ctctagttta ggagtcgctt ttgtaattat
     6361 gggagcggga tttttacctg cctttttagt ttctgattac ggaattgtca tcgcatcttc
     6421 cttgggaagt tttttagttt taatggctgt tttggtagtg tcacaaaaat taagggacac
     6481 gatggctatt ttgattgttg gactcatgtt tggcagtttc actagtgcca tcgtcagtgt
     6541 gttgagttat ttcagcaccg cacaggaatt gcaaaaattc actttttggt cgatgggaag
     6601 tttagctaat ttatcgtgga actccgtatt gattttagct atttgctgta gtttcggatt
     6661 actgttgagt attttgagca tcaaaccttt aaacgcttta ttgttaggtg aaaactacgc
     6721 taaaagttta ggtttgaatt ttaaacgaac gcgattcatc atcatttttg ccactagtat
     6781 tttggcagga agtatcactg catttgccgg tcccattgca tttgtaggtt tggcagtgcc
     6841 tcatttggca aagttgttat ttcaaaccag taatcatttt atattgtttt ggagtaccct
     6901 tttattagga gctattttaa tgttggtttg cgacagtatc gctcaacttc ccggaagtga
     6961 ttacacacta cccatcaacg ctatcacatc gatggtagga gctcccgttg tggtttggtt
     7021 attagttcgc aaaagaaaaa taatgattta gtataaacca catagaaaca tagcttatga
     7081 taacgcaaaa atatttagat gaattgactt atgaagttgt tggttctgca atagaagttc
     7141 ataaaattat ggggagaggt ttgttcgaaa gtgtttatca tcaatgttta attgaagagt
     7201 tgaaacatag aaaaatatat tttttaacag agatgaaagt tcctgtaatt tataaaggaa
     7261 aatctttaga tattaatttt agatgtgatt tgtttattga gaattgttta gtagttgaaa
     7321 ttaaatccgt tattgactta cataaaatta ctgaagctca actattaaat tatatgaatt
     7381 tattaaaagc tccaaaagga attttaatta attttaattg tagaaatatt tttaatgaag
     7441 gacaaaaaac ttatgtaaat gaatatttca aaaaattacc acaatactaa tgctataaat
     7501 aaagcttgct ttatctctta tgttttaaag tatagatatt cctatgtttc tatgtggtta
     7561 aagtaacaaa aaccaaagca cctaaaaatg attctccaaa cacaacatct cagcatcggc
     7621 tactcttcaa aggctcaaac aaaagtgatt gcttcggatg tttctattca attggaacaa
     7681 ggaaaattaa ttggattagt tggagcaaac ggaattggaa aatcaacgtt gttgagaaca
     7741 ttaaccggta ttcaaaaatc actttctggc acagttttat tgaacgaaaa accaattgat
     7801 tcgtttgaac cgttggcttt agcacaacaa attagtgtag ttttgaccga aaaattgcca
     7861 ccgagtaatt taacggttta tgaattgatt gctttgggaa gacaaccgta tacgaattgg
     7921 ctgggaaaat tagcagagga agataaaatt caaattgaaa atgcaattag tctgactgaa
     7981 attggtcatt taattcatca aaaatattac gagatcagcg atggacaatt gcaaaaagtt
     8041 ttaattgcac gggctcttgc tcaggacaca ccgttgatcg ttttagatga acctgctacc
     8101 catttggatt tgttgcataa agtttcactg tttaaattat tgaaaaaact aacagtagaa
     8161 actcaaaaat gtattatgtt ttctacgcat gacattgatt tagccataca attgagtgat
     8221 gaaatgattg taatgacaaa tgaaaaagtg atacaaaatc agccttgcaa tttaatttta
     8281 gacggtacat tttctaattt attcttgagt gaaaatattc tttttgattc agaaaaagga
     8341 aaatttatta ttcgataaac ttatctttta agtttaaaaa tccatttcta ttttctattt
     8401 ttactttctt caaaaataga aaatatgaca acttcttttc ttcttctact aacctttgtg
     8461 cttgctttat ctattggaat atttattggt aaaatgcttt ctacagcaca atcgaaatct
     8521 gaaaaatcag ctttggaaga acgaataaat ggacttttaa ttcaattaga tcaatcaaaa
     8581 aatcgttttc aggaagagca aaatcaattt gtaaaacaac tagaaagtga aaagaaagag
     8641 aaggaagaaa ttcgtcggga aaaagattcc ttatcgttac aactcaccaa aaaagaagct
     8701 gattttgaaa atctttggca acgcaataaa gaacaaaagc aagaagtaga agagttgcag
     8761 gaaaaattca ccaaagaatt tgaaaactta gccaataaaa ttctcgaaga gaaaacagta
     8821 aaatttaccg aacaaaataa agaaaatcta aaaaatattt tatcgccttt gcaggaaaaa
     8881 attcagcatt ttgaaaagaa agtcgaagac acccataagg aaagtatcga ttatcatgct
     8941 gcattacgtc agcaaatagt tgggttaaaa gaaatgaatg agcaaatgag taaagaaacc
     9001 cttaatttga caaaggcttt gaaaggtgac agcaaaatac aaggaaattg gggtgaattg
     9061 attttagaac gcgttttgga aaaatccggc ttagaaaagg gcagagaata tgaagtacaa
     9121 caaagtttta ctaatgaaga aggtaatcgt gtttttccgg atgtggtaat taacttacct
     9181 gacggaaaga aaatgattgt tgattcaaaa gtttcgctta ccgcttatga acgttacact
     9241 aatgaggaag atgatttgca aaaaagcagt tttttaaagg aacatatcaa ctccattaaa
     9301 cgccatgtgg agcaattgag tgataaaaat tatcatgatt tgtaccaaat ggaaagtcct
     9361 gattttgtct tactgtttat tccaatcgaa tccgcttttg caatagcttt gaatgaagat
     9421 aattcgttgt acaacaaagc ttttgaacgt aatattatta tcgttacacc tacaacatta
     9481 cttgctacat tgcgaacaat tgacagtatg tggaccaacc aaaaacaaca ggaaaatgcc
     9541 ttagagattg ctcgtcaagc cggagcattg tatgataaat ttgaaggatt tgttacggat
     9601 ttggttaaga ttggtaaaaa aatggacgaa gcaaaagtgg aatatggtaa tgccatgggt
     9661 aaattagtag aaggaaaagg aaatttggta ggaagtgtcg aacggctacg aaaaatgggt
     9721 gctaaggcta aaaaagtact gcctgaaaat attcttaaaa gagccggaga ggaattagag
     9781 gaagagtgag aattgtataa tcttgaaaat caattttaag taattttttt ataagtaaga
     9841 aaattaataa atttaaacat tactatttca tttttaggtc attaatttca tttttttata
     9901 ctaaaaagtc aaaactattt gattaaaaat gtgaatcata tcgttttttt gttatttttg
     9961 gaacaaattc caaaacaaat aatgaaaaaa aattacgctt accttttacc cttatttatt
    10021 gcaattgctt ctttatttat tgcagtttct tgttcaaatg acgatgatta tgttccgatt
    10081 gtagatgttt tgcctgttgc aacagatgat acagctactt atgcgttagc agaaacagtt
    10141 gaaataccgg ttttgttaaa tgatacaacc ggtgacgaag taattccaac taccgtcagt
    10201 ttagttggtg gaattgatac agattctaat acaactttag atgaattgac aattccggga
    10261 gaaggagttt ggactgttaa tgcgttgaca ggagttgtta cttttacacc gaattcttcc
    10321 tttcaaaata atcctactcc cataaaatat actgttgaag atttagaaga aaatatttcg
    10381 aatcaagcta ccattactat tacagcagta caaacagctg atgttgactt aacacaagtt
    10441 ccttatcaaa aactttctga ttataagttt tttgccggag aaatgaaaaa tcagattcct
    10501 tctttgggag tattacccta tgaaccggct agtccacttt ttactgatta tgcccataaa
    10561 aaacgttttg tttggatgcc aaatggtgta aaagcaacct atgaaggcga tggtaattta
    10621 ttagaacttc cggtaggatc tgttattatc aaaaacttct attatgataa tgtacaaccg
    10681 ggcaatgtga cccgaatcat tgaaacgaga atgatgattc gtaaagcaga agaatggatt
    10741 tttgcagaat atgtgtggaa tactgaacaa acggaggctt ttttagacat gaatggaagc
    10801 aataccagta ttacttggat tgatgaaaat aatgttacta aaagcacgtc atcttacaga
    10861 ataccctctg ggccacaatg tatgacctgt cataaatcca atgaagtggc cgttccaatt
    10921 ggtataaaac cacaaaatat taattttaac tatacctatt caaccggctc taagaatcaa
    10981 ttgtcaaaat ggattgaaca aggttatttg gaaaatgatt tacccggaaa tattgtttct
    11041 accgtagatt atagagacac ttcaaaacca ttggaattaa gagtgcgttc ctatgttgat
    11101 atgaattgtg ctcattgtca tcgtaacaat agtcattgtg attaccgacc tatgcgtttt
    11161 gcttttggag aaacaacaaa cttagtaaac atgggcgttt gcgttgatac ccaagatatg
    11221 gctggtttcc catctgcctt gagtaaaata gtaacaccgg gaaatagcaa tcgctcaatg
    11281 ttgcattata gattaaatac agctgaccca gcttatatga tgccccttat tggaagatca
    11341 atgatacatg aagagggagc agccttgatt caggaatgga taaattcatt atcaggacct
    11401 tgtttataaa aaactaaatt aactaaacta tactaatttt aaccaaatga agaaaattac
    11461 ttatttaatt cttttttgca taggaattca actttccaat gcacaagata catgtgcttc
    11521 ggccgcagtt ttgccgggag cagggctcta cattgttgac gccgttaatg gtttagaggt
    11581 tccaaatcca gtttgtactg agaatggtaa catcgctaat aatggaaaag gcgagtggta
    11641 ctcttacaca ccaacacaaa attatacaat aaccattaca acggatattg ccgctaatac
    11701 gccaagagta gacactcgtt ttcatgtgta taccggtact tgcggagcat taacttgtta
    11761 tgccggtgat gatgatgaag gaatcggtga tacaaattac tcttcaattg atacttttgt
    11821 ggttttagct aatactactt ataccatagc ttttgataat cgatggactg ctaatgggtt
    11881 tacttttcaa ttgattgagg ctccatatca agagccaatt attaatcctg ttacttttac
    11941 aaaccaagcc attgcttccg ttaatagtca atataatatc actgtggtag acatgaacgg
    12001 tgattattta gatgatattg ttggtgtaaa tcaggacaat attcgtattc atactcaaaa
    12061 tgccggtgga acttttaccg ttaccgatta tccaactact tcagcgacaa atatgccttc
    12121 ttggagttta gcagcagcag attataacaa agacggtttg aatgatttga tgtatggtgg
    12181 gggtaatggc gttactttta tgaaatcaaa tgaagcagga aatggatatg atcaaaatac
    12241 tttttctgaa tacgtttttt gtcaacggac taatttcgtt gatataaata atgatggtca
    12301 tttagatgct ttttcttgtc atgacgttca gcctaatgtt tatttcctta atgatggttg
    12361 gggaggtttt accttttatc aatcaacggt tacacccgga gctattctat taggaattca
    12421 tccaaatgga ggaaattatg gttctatttg gattgattat gataatgatg gtgaccaaga
    12481 tttgtttatt gccaaatgtc gtggtggttc aggaacagct aaattcaatg aattacacag
    12541 aaacaatgga gatggaacct ttacagatgt ttctactgca gcaaatatgt atgatccggt
    12601 tcaaacttgg tcatcagcat gggctgatta tgataatgat ggtgatatgg acgcacttat
    12661 tggtgcaagt tctaccactg atggaactca taaatacatg agaaataatg gcgatggaac
    12721 ttttacagat attacagccg gttcaggatg ggatattaac gcgagtacta atatcgaaca
    12781 tattgctcat gattttgata atgatggatt tgttgacgta atgggtggtg gaaacaaaat
    12841 tatgtacaat aaaggtgatg gaacgtttgc tccatcaggt gttggttttg gtgtaagtcc
    12901 ggtaggagat ttaaataatg atggtttcat tgattttcaa accggtgcta cattaagaat
    12961 caataatgga aattctaata agtggataaa attaaattta cagggtattc aaagtaaccg
    13021 caacggtatt ggagcaagag tagaaattta tggtgcttgg ggaaaacaaa ttcgcgatgt
    13081 tcgtagcgga gagggcttta aatatatgag ttctttgaat gtacatttcg gaattggtca
    13141 agctacagca attgatcagg tgattatcag atggccatcc ggtcttgttg atactatagt
    13201 aaatccttct cctaaccaaa ctttattagt ggttgaagga gctacattga gtgttaacca
    13261 aaacactatt tctaattttg tgttgtatcc taatcctgca aaagattttg taaccttgaa
    13321 aagttcaaca acaagttcaa tagtttctgt tgaaattttt gatttaaacg ggcgtaaagt
    13381 tttaacttct gatgtagtaa aagaaacaat ttctgttcaa tctttatcgg tagggactta
    13441 cctgctttta gcaaaagaca gtgatggaaa tctttctaca cagaaattta tcaaacaata
    13501 aaaaaaaagc aaccaaacta aattttgaaa aagccctccg ttgtgagggc ttttttataa
    13561 ctttgaattt caaaaaataa cacaaacaac atgaactaca tactttttga cggaccggtt
    13621 cgtaacgctc ttttgccttt tacattcact cgtccggtag ctgatattcg catcggaatt
    13681 ttaacgatac gtgaaaaatg ggaaaaatat ttaggctata ccaccacaac cattaccgaa
    13741 gaatatttat ctgaaaaata tccaatggtt gaaatggagg aaaatattct catcaatgcc
    13801 tcatttttac ctaacgaagt attggtggaa atgattaaat cgttagaaaa aaaccaagcc
    13861 atttttaaag gggaagaagt tgtcgctttt ttcacaaaca atacacaaga agaagtagat
    13921 tttgatacct acgaaatcat cgattacaac gaagaaggtt tacgtatcga acacacttgg
    13981 gacatttttg ctaagaatga tgccgccctc cgtgaagatt ttgaattgtt aaccgaagga
    14041 agaacttcac aaccgattcc taaaagtgtg aatgtaatta gccctgaaaa tatatttatt
    14101 gaaaaaggag caaagcttga atttgttact ttaaatggtt ctacaggacc gatttatatt
    14161 ggctataatt ccgaaatcat ggaaggttct gtgattcgcg gcccatttgc cttatgtgaa
    14221 gaagcccaag tgaaattagc tactaaggtt tatggagcca cgactgttgg accacattgc
    14281 cgaataggag gtgaagtaaa taattctgtt ctgtttggct attccaacaa aggacacgat
    14341 ggctttttgg gaaatgccgt gttaggcgaa tggtgcaaca ttggtgctga ttccaataat
    14401 tccaatttaa aaaataatta tgatgaggtg aaattatgga gttatgaaac ggaaggtttt
    14461 gaaaaaaccg gtttgcagtt ttgtggactc atgatgggcg atcacagtaa atgtggcatc
    14521 aacaccatgt ttaacaccgg aacagtcgtg ggcgtttcta ctaacatttt cggaagcgga
    14581 tttccacgca attttgtacc gagtttcagt tggggaggtg cagccggatt ttcaacgtat
    14641 atcaccaaaa aagcctttga aactgctcgt attgtaatgt ctcgccgaca tgtggaattc
    14701 accgaacaag atgcgagaat tttagaacat gtgtttgagg agacgaagaa gtggaggaag
    14761 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaagatgcg agaattttag
    14881 aacatgtgtt tgaggagacg aagaagtgga ggaaggaata attctttttg gtttctcgca
    14941 aagacgccaa gtcgcaaagt cgtaaaatga ttatcctttg cgtctttgcg agaaattaaa
    15001 acatcaattc ttttttaccc tcttcaaact taacaaattc aacggattaa tccccaaacc
    15061 ttccacattt tttctcgtaa accttgccac tttatcataa aacaacggaa taattggagc
    15121 ttcttcaata atgattttat ccatttgttg gtaaaattca aatcgcttta catcatcggt
    15181 ttctaaaaaa gctttttcat acaaagcatc aaatttggca tttgagaaat gcgtataatt
    15241 cggtccattc ggagcaaagt ttttactgta aaaaagcgat aaataattct ccgcatcggg
    15301 ataatcggca atccaactgg ctctaaaaaa tgtcactttt ccggtcgcaa tcgcttgtcg
    15361 taatgttgag ggtgggttga catcaatctc aacatctaac ccaatttttt gccattctcg
    15421 ttgtaaaaat tcaccaatat caatgtaaga ggcatttgtg ctcaaaaaga tttttggttt
    15481 tttgacaccg gtttctttga caaattttgc cactaaagat ttagcctttt caggttcaaa
    15541 atcataccct ttttgattat taaaactggg cattccactc ggaatcacac cgctggttgc
    15601 cggaattccc atgttgtttc gcaaataggt aatcattttc gctcggtcaa aaccatagtt
    15661 taaggcttgt cggatgcgtt tgtctaaaat agttttatct gttccatcca ttctaaaacc
    15721 taaatattcc gtgttgaggt agggtccgct aatcaaatta acatcatctg catatttcgg
    15781 ttgtaattca cctttttgcg tcagaatttc atctttatac gatggatcca aaccggagat
    15841 aaaatcaatt tttccttgaa taaattctaa aaagccactt tgtttgtcgg gtaaaaaggt
    15901 aatcgcaacg gcttccaaat aggggagttg aatgcctttt tcatcttttt caaaataaat
    15961 cggatttctt ctcaaaacta attttacatt ttcttcccaa aatttaaatt gaaacggtcc
    16021 ggttccgata ggcttggttc tgaaatcata attcgaactt tctatgattt ccttcggaac
    16081 aaccgacgca tatttcattg ataataatcc taaaaatgcc ggaaaagatt gggttaattt
    16141 tatttcaacc aacgtatcat ttaatgcttg aatcttttcc acattttgca aaatccaccc
    16201 gccgggtgaa gccacttttt cgtctttcaa tcgttttaaa ctgtattcga aatcactggc
    16261 tttaactgtt cgtgtagaat cttttccaaa aaggatgtgt ttgtgaaaat aaatatcttt
    16321 tcgcaacaca aattgataag ttttcccatc ttctgaaatc gtccaacttt tagcaatatc
    16381 cggttgaatg tgcaagctgt catccaattg aaccaatccg ttaaacaatt gattacaagc
    16441 ccaaataatg ttttgcgatt ttgaaaaagc ggggtcaaga gtggcaatgt ttgcactttc
    16501 gttgtatcta aatactaaaa gttctttgtt gttttctttg gaatcatgac aagaaagcaa
    16561 taatagctga agcgatagag ttacaaggaa aagaattttt ttcattaagc aattattgaa
    16621 tgtagataat tgatgtaaag ttagcataaa ttgaaaattg gaatactaaa gtttaaaaaa
    16681 tctgtgtaaa tttgcagcct taactttttg acaacgattt cattagttaa gctattttat
    16741 ggaacacaaa aagaaagtag cattctacac tttaggatgt aaattaaatt tttcagaaac
    16801 ctccactatt gccagaaact ttcaacacga aggttttgaa cgcgttgact ttgaagaagt
    16861 tgcagatatc tatgtcatca atacttgttc ggttacagag aatgccgaca aacaattcaa
    16921 gcaaattgta aaaaaagcat tgaaaaataa taataaagcg tttgtggctg ctgttggatg
    16981 ttatgcccaa ctgaagcccg aagaattggc tgctgttgac ggagtcgatt tagttttggg
    17041 agccacagaa aaattcaaaa tcaccgatta tttaaatgat ttaagtaaaa atgacttagg
    17101 cgaagtacat tcttgtgaga ttgaagaagc caatttttat gtgggaagtt attctattgg
    17161 cgatcgaacc cgtgcatttt tgaaagtgca agacggttgc gattataaat gtacgtattg
    17221 cacgattcct ttggctcgtg ggatttcacg cagtgatgaa ttgcaaaatg tattgaaaaa
    17281 cgccaaagaa atttcgcaac aaggcatcaa agaaatcgtg ttaaccggtg tgaatattgg
    17341 tgattatggc aaaggtgaat ttggtaataa aaaacacgaa catactttct tagaattggt
    17401 acaagcgttg gatgaagtag aaggtattga acgtttgcga atttcctcca tagaacccaa
    17461 tttattgaaa aatgaaacga ttgattttgt atccaaaagt cggacgtttg tgccgcactt
    17521 tcatattccg ttgcaatcgg gaagtaacga cattttaaag aaaatgaaac gtcgctattt
    17581 gcgtgaattg tatgtggacc gcgtgaataa aattcgcgaa gtcatgtcgg atgcgtgcat
    17641 tggtgtggac gtgattgttg gttttcccgg agaaacagag gagcattttt tggaaaccta
    17701 tcattttttg aatgagttga acatttctta tttacatgtg tttacttact ctgaacgtga
    17761 caatacggag gcagctgaaa tggaaggaat cgttcccgga aatgtacgtt cgaaacgcag
    17821 caaaatgctt cgtggattgt ctgttaaaaa aa
//
LOCUS       DAOTCW010000041        17756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1765_length_17756_cov_41.262584, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000041 DAOTCW010000000
VERSION     DAOTCW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 17756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17756
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1765_length_17756_cov_41.262584"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /gene="ettA"
                     /locus_tag="P5335_05030"
     CDS             complement(<1..1457)
                     /gene="ettA"
                     /locus_tag="P5335_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRZ74277.1"
                     /translation="MADDKKVIFSMSKVSKTYSSNNKQVLKDIYLSFFYGAKIGILGL
                     NGSGKSSLLKIIAGIDKNFQGDLVFAPGYKVGYLEQEPQLDETKTVIEIVREGAAETI
                     ALLDEFNKINDMFGLPEVYEDADKMQKLMDRQAELQDRIDAAGAWEIDNKLEVAMDAL
                     RTPDSDTPIKVLSGGEKRRVALCRLLLQQPDVLLLDEPTNHLDAESVLWLEQHLQQYP
                     GTVIAVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLDQKSKRMELEEKVASKRRK
                     TLERELDWVRQGAKGRQTKQKARLQNYDKLLNEDQKELDEKLELYIPNGPRLGTNVID
                     ANGVSKAFGDKLLYEDLNFTLPQAGIVGIIGPNGAGKTTIFKMIMGEETPDKGTFVTG
                     ETVKIAYVDQAHSNIDPEKSIWENFCEGQELIMMGGRQVNSRAYLSRFNFGGSDQNKK
                     VSTLSGGERNRLHLAITLKEEGNVLLLDEPTNDLD"
     gene            complement(1479..1661)
                     /locus_tag="P5335_05035"
     CDS             complement(1479..1661)
                     /locus_tag="P5335_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAL67264 family membrane protein"
                     /protein_id="HRZ74278.1"
                     /translation="MGMNKNTVLAWATFIMIIMGLLLIGMGAFKYDDVAGWGFASTGI
                     GFLAIAWVFNALKGRV"
     gene            1831..>2126
                     /locus_tag="P5335_05040"
     CDS             1831..>2126
                     /locus_tag="P5335_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HRZ74279.1"
                     /translation="MAFTPLKFKLLLIFFIFTGITLIAQENRLKNIQKLTFGGDNAEA
                     YFSPDGKKLTLQVTNKAFGVPCDQIFMLDLQEKEFSEKSLKLISTGTGRTTCSY"
     assembly_gap    2127..2226
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2227..3915
                     /locus_tag="P5335_05045"
     CDS             <2227..3915
                     /locus_tag="P5335_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRZ74280.1"
                     /translation="ELWTMDIDGSNLKQITFGLGYDGGCFFSHDSKKLVFRSSRPKTE
                     EAIAEYKALLAENLVAPSDMEIHTCNVDGSDLKQITHLGKANWAPYFHPSDKKIIFSS
                     NHHSTRGYDFQLYMIDIDGENLQQITWESEFNAFPMFSPDGKKLVFSSNRQQEKARET
                     NVFIADWVDGDVAELAQPKSLKKNISYLASDELKGRLTGSKEEQMAADYLVKELKSLG
                     IKPYEGKNYIQKFDYKVRLNPHDSLDLKNNTGRNVIGFLDNKATKTIVIGAHYDHLGL
                     NEHNHSAKPNSKGEIHNGADDNASGVAAVLELARMFSKNKTIEKVNYIFAFFSGEEDG
                     LIGSKFMTSLLKNYHSNVVSMINLDMIGRLNDSKNLTVGGVGTNPNFKTIVDKNKPAG
                     FNVTLDESGVGPSDHTSFYLKDIPVLFFFTGTHTDYHKPSDDEVKINYYGVRNITDYV
                     FRVSNAIADLDKVEFVKTKIDAPKTAPKYKVTLGIMPDYTDHGDGLHVDGVTDNRPAQ
                     LAGIQSGDIITKIGDCEIKEVYGYMKCLSGINAGDELPVTFLRNGETMTAKVKF"
     gene            complement(3917..4921)
                     /gene="holA"
                     /locus_tag="P5335_05050"
     CDS             complement(3917..4921)
                     /gene="holA"
                     /locus_tag="P5335_05050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRZ74281.1"
                     /translation="MEEIAKIIKDISGGNIKPIYFLMGEEPYYIDKLTEFIESNILSE
                     DERAFNQQVLYGRDVSIEDVISAAKRYPMMADRQVIIVREAQELSRNIDKLESYVKNF
                     QPTTVLVIAYKYKSLDKRKSMTKLIEKEGLLFESKKLYENQIGTWISRVLQGKGYHIE
                     PKATAMLVEFLGTDLSRINNELEKLQIILPKGTTFTPTHIEENIGFSKDFNVFEFRKA
                     IGERNQLQAYRIADHFAQNKKEYPMIFTTGMVFSFFSSLLQYHGLKDKSPGNVAKVLK
                     VNPYFLKDYDLALKNFPMKKVSAIVSTLRDIDVKSKGVGANQLHESDLLKEMLAKIFN
                     "
     gene            4984..5436
                     /locus_tag="P5335_05055"
     CDS             4984..5436
                     /locus_tag="P5335_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HRZ74282.1"
                     /translation="MQKLNFSDYSFRFKNSENKVSVFDEIRKKFIVLTPEEWVRIHVV
                     QYLIQEKNYPKSLINVEKLIKINTISKRYDIVVYQPNGEIFLLIECKKPDVSINQLVF
                     DQIARYNLTLKAKYLMVTNGLNHYFCQMDFEQERYVFLKDIPDYELQN"
     gene            5467..6477
                     /locus_tag="P5335_05060"
     CDS             5467..6477
                     /locus_tag="P5335_05060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRZ74283.1"
                     /translation="MKKIAIVILNWNGAKLLQQFLPSVLSYSQEATIYVADNASTDES
                     ISVLKTQFPSIKIIKNSSNFGFAQGYNECLKFVEEEIYCLVNSDIEVTENWLAPILEL
                     FDKNEKVAIIQPKILDFKEKAYFEYAGAGGGFIDRFGYPFCRGRIFDSIEKDNGQYDD
                     VKMIFWASGACFFIRKKVFRELEGFDADFFAHQEEIDLCWRSHNLHYETYYCGFSKVY
                     HLGGGTLSNTNPRKTFLNFRNSLFMLVKNLPTVHLIWILLIRMILDGIAGIQFLFQGK
                     FSHFTAIIKAHFYFYALLVKMFRKRKNNQQSNYYFTTSIIYHYFIKKEKIVVDKFNNS
                     LS"
     gene            6540..7376
                     /locus_tag="P5335_05065"
     CDS             6540..7376
                     /locus_tag="P5335_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRZ74284.1"
                     /translation="MKKVVLLLALVALSLGSCGTKKKIAELESKNKECQDLLNSTTVK
                     LNMCLSEKDILNNQIADLKKTNSDLISNVGNMTMLSTKGAENLEKSLESLKEKDLKIS
                     RLQDALTKKDSVTLALVTSLKSSVGINDPDIEINVEKGVVMISIADKMLFKSGSYEVS
                     DRAKEVLGKVAKVVNDKPTFECMVESHTDNVPYRNGVLLDNWDLSVKRATSVVRVLTN
                     DHKVSPKQLIAAGRGEFIPLVANDSTQNRSANRRTRIIVMPKIDEFYDMIEKEMKNQA
                     GK"
     assembly_gap    7431..7530
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7544..8164)
                     /locus_tag="P5335_05070"
     CDS             complement(7544..8164)
                     /locus_tag="P5335_05070"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRZ74285.1"
                     /translation="MSQFIKIYPENPNEAAIAKVVKVLKEGGLVIYPTDTVYGLGCDI
                     TNTKALERIAKIKGIKLEKANFSFICHDLSNISDYIRQIDTATFKILKRTLPGPYTFI
                     LPGNNNLPKEFKKKKTVGIRVPNNAIVLEIVKLLGNPIVSTSIYDEDEVLEYSTDPEL
                     IFEKWQNKVDLIIDGGYGDNQPSTIIDLSEGEPVVIREGKGDSNIF"
     gene            complement(8283..8720)
                     /gene="rplI"
                     /locus_tag="P5335_05075"
     CDS             complement(8283..8720)
                     /gene="rplI"
                     /locus_tag="P5335_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRZ74286.1"
                     /translation="MELILKQDVQNLGFKDDIVSVKPGYGRNFLIPQGFAALATPSAK
                     KVLAENLKQKAHKEQKVVDDAKKIAEALKALDIKISAKAGGEKLFGSVTNIDIAEALE
                     KAGQPIERKFITSGTVKRTGKYTATVRLHRNVVVELPYEIVAE"
     gene            complement(8782..9078)
                     /gene="rpsR"
                     /locus_tag="P5335_05080"
     CDS             complement(8782..9078)
                     /gene="rpsR"
                     /locus_tag="P5335_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRZ74287.1"
                     /translation="MSTIEQSAKGKKEGDIRYLTPLNIDTNKIKKYCRFKKSGIKYID
                     YKDADFLLKFVNEQGKILPRRLTGTSLKYQRKVSVAVKRARHLALMPYVADLLK"
     gene            complement(9084..9422)
                     /gene="rpsF"
                     /locus_tag="P5335_05085"
     CDS             complement(9084..9422)
                     /gene="rpsF"
                     /locus_tag="P5335_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRZ74288.1"
                     /translation="MNHYEAVFILNPVLSEQQVKETVSKFEDFLTAKGAQMVSKEDWG
                     LKKLAYEIQHKKSGFYHLFEFKAPAESILSFETELRRDERIMRFLTVSLDKHAISWAE
                     RRREKLKVKA"
     gene            9591..10286
                     /locus_tag="P5335_05090"
     CDS             9591..10286
                     /locus_tag="P5335_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRZ74289.1"
                     /translation="MKLNCVVVDDSSIQRMIITKLVNNHQNLNLVGDFSNAIEAKSCM
                     SIHSVDLIFLDIEMPIINGFDFLDGLKQKPQIIFITSKADYAVKAFDYDATDYLQKPI
                     SVDRFDSSVKRAIELHKLRHEVTEEEGDYIFIKSNLKKLKVFTSKIKWIEAYGDYVKV
                     VTDEDSNLVLSTMKSFENDLSKDRFLRVHKSFIVNIDKIERFNSKFAEIGVTKIPLSR
                     NKKDDLKKALAIA"
     gene            complement(10283..12733)
                     /gene="priA"
                     /locus_tag="P5335_05095"
     CDS             complement(10283..12733)
                     /gene="priA"
                     /locus_tag="P5335_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRZ74290.1"
                     /translation="MAYFIEVILPLALDKTFTYKVTEAEYYFIKPGMRIAVPFGKSKI
                     FTALAIEVHQNPPTLYEAKEIHQILDENPLVNEIQLKHWQWISTYYMCSIGEVFRAAL
                     PSTLLLESETIISSKHNEAVEELNLTDDEFLIFEALQHQSSLKVNDVMTILNKKNIFP
                     LLQKLIDKNIITLQEEIQENYKPKLVKYIKLHQNYTSNESLNELLNSLKAKKQSELLL
                     GYFQLQAVEKKPISAKQLLEFSKVSSAVLKVLVDKAIFEEYHLIQDRTDFDALDLNEN
                     LVLSPAQQKALDEIKLQFEQKEICLLHGVTSSGKTEIYCQLIQEMMKTDKQILFLLPE
                     IALTTQLLSRLRKFFGNKVAVFHSKYSNNERVEVWNQVLENSPKAQVVIGARSAMFLP
                     FSNLGLVIVDEEHEQNFKQSDPAPRYHARDAVTVLATFHKAKVLLGSATPSIETYYNS
                     QVHKYGLVELFERYNNVPMPEIILVDLKDKFFRKRMNGHFSDTLIEEITSALSLNEQV
                     ILFQNRRGYAPLLECMTCGHIPQCPNCDVSLTYHKYKNQLRCHYCGHSIAKPTNCHQC
                     SSVDLTTKGFGTEQIELELATLFPNRKIQRMDQDTTRGKFGYEKIIDKFQNREVDILV
                     GTQMLAKGLDFDNVSLVGILNADNMLYFPDFRAFERSFQMMTQVSGRAGRSEKKGKVI
                     IQTFNPNHNTIQQVTHHDYLAMYKEQLYERQIFKYPPFFKLIKITLKHKDFEKVKESS
                     MWLYQVLNQQLNMPVLGPEEPAISKIRNEYIRTIMIKIPDTVSPQGTKKTIQKILNSF
                     DVISQYRAVKLVVNVDVT"
     gene            complement(12775..13701)
                     /locus_tag="P5335_05100"
     CDS             complement(12775..13701)
                     /locus_tag="P5335_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRZ74291.1"
                     /translation="MSLEIEEKIDKIPIVKYVVRLMKRIKLPWLEGLSFYDLMELYII
                     GIVEGAFSYRAGAIAFSFFMALFPFALFILNLIPYIPIEGFQDDFLKFVEDGVPPNTF
                     EAIKTIIDDILNNSYQGLLSSGFILSIFLMTNGINAVLGGFETSYHITINRSFIRQYI
                     IALGMSLLFSFILIITIVAILISEIIIQKIHLHKIINTDITLVEMIRFGFVVLMILST
                     TSLLFKFGTKQISKVSFISIGSVFTTLLILLSSYVFGIYVVKFAKYNELYGSIGTLLI
                     LMFYIWINCMILLLGFELNATINKLKRKNLFD"
     gene            complement(13728..14585)
                     /gene="nadC"
                     /locus_tag="P5335_05105"
     CDS             complement(13728..14585)
                     /gene="nadC"
                     /locus_tag="P5335_05105"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRZ74292.1"
                     /translation="MITEVQFQKELELIIKNGIREDIGAGDHSSLACIPKSEQGKAKL
                     LVKDSGIIAGEEFAKMIFNEVDSNLKIETFIPDGSLVKYGDVVFHVSGSSQSILKAER
                     LVLNSMQRMSAIATKTKKYVDLLDGTATKILDTRKTTPGFRAAEKWAVKIGGGENHRF
                     ALYDMIMLKDNHNDFAGGISLAIQKTKDYLIQNELDLKIIVEARNLKEVEEILSSGGV
                     YRILLDNFDFETTKKAVEIIGNKCLTESSGNINETTIQQYAACGVNYISSGALTHSVY
                     NMDLSLKAF"
     gene            complement(14700..14915)
                     /locus_tag="P5335_05110"
     CDS             complement(14700..14915)
                     /locus_tag="P5335_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRZ74293.1"
                     /translation="MKKNVGNTDRFVRVIIGIILLIVFMSGFIENDMIKWIVLGVSSI
                     LIITAFATFCPLYALLGKSTCEVKKKK"
     gene            complement(14986..15300)
                     /locus_tag="P5335_05115"
     CDS             complement(14986..15300)
                     /locus_tag="P5335_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRZ74294.1"
                     /translation="MNLQQEQWWAEAQKDENAVFLDVRTPEEFEHGIIPNAINIDIYK
                     GQGFIYLLDELDKAKSYYVYCHAGSRSAKACEIMRQMGFEKTFNLVGGMSNWKGLIVE
                     PN"
     assembly_gap    15307..15406
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15525..15980
                     /locus_tag="P5335_05120"
     CDS             15525..15980
                     /locus_tag="P5335_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRZ74295.1"
                     /translation="MKIEEIIKSNSLISNEKRTVLNIMFTQNIVADAFNEILKTFDLS
                     VEQFNVLRILRGQKGKPANMCVIQERMIAKTSNTTRLVDKLLLKGLVNRVVCEQNRRK
                     MEITITERGLQLLNELDPKIEAHELLFANNLTKQELENLNELLEKYRTI"
     gene            15991..16623
                     /locus_tag="P5335_05125"
     CDS             15991..16623
                     /locus_tag="P5335_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRZ74296.1"
                     /translation="MSNFITDQKWRYATKKFDASKKIAASDLETLKEAIQLSTSSYGL
                     QPYKIFIIENAELRAKLQPAAWGQSQIVDASHVIVFANIVNFGDAEIDAYINNLAETR
                     GIEIESVKGYADFIKMKINALSVEKRDVWTSKQTYLALANLMNAAAELKIDVTPMEGF
                     EPEAVNEIMGLKKLGLNASLIATIGYRHNEDLTQHYAKVRKPKSELFINL"
     gene            16649..17218
                     /locus_tag="P5335_05130"
     CDS             16649..17218
                     /locus_tag="P5335_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010521129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRZ74297.1"
                     /translation="MKNFKTIALALVVALGTFSATAQNKKINVEKSSIKWIGKKVTGQ
                     HDGTINFGSGTLVFEGKKLKGGSFEVNMTSIAVSDLEAGKGKEKLEGHLKSGDFFGVE
                     KYATSTLEFKKIGSKGSDIYTVTADLTIKGITVPVTFDLALGDGTARTSFKINRTKYD
                     IKYGSGSFFDNLGDKAIDDEFELNVNLVY"
     gene            17320..>17756
                     /locus_tag="P5335_05135"
     CDS             17320..>17756
                     /locus_tag="P5335_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRZ74298.1"
                     /translation="MKKVLVIDNYDSFTYNLVHYLEDLNCEVTVFRNDEFELHEVEPF
                     QKILLSPGPGLPKDAGLLNQVIKKYAPQKSILGICLGLQAIAEVFGGNLLNLIKVHHG
                     VSSQITIIQEDVLFDGLPKNLQVGRYHSWVINTENIPEVLEVTS"
BASE COUNT         5906 a         2833 c         2705 g         6012 t
ORIGIN      
        1 atgtccaaat cattcgttgg ctcatctaat aacaatacgt ttccttcttc ctttaaagtg
       61 atggctaaat gcaaacggtt gcgttctcct cctgaaagtg tggacacttt tttgttttga
      121 tcactaccac caaaattaaa acgacttaaa taggcccttg agttgacttg tcgaccgccc
      181 atcatgatta attcttggcc ttcgcaaaag ttttcccata tcgatttctc cgggtcaatg
      241 ttggaatgtg cttgatcaac ataagcaatc tttactgttt caccggtaac aaatgttcct
      301 ttgtccggag tttcttctcc cataatcatc ttgaaaatag tggttttacc ggctccattt
      361 ggaccaataa tacccactat tccggcctga ggtaaggtga aattcaaatc ttcatataat
      421 aatttatcac caaatgcttt cgaaacaccg ttggcatcaa tcacattagt tcccaaacgc
      481 ggtccgtttg gaatatataa ttctaatttt tcatccaatt ctttttggtc ttcattcagc
      541 aacttgtcat agttttgcaa acgtgctttt tgtttggttt gacgaccttt agcaccttga
      601 cgaacccaat ctaactctct ttctaatgtt tttctgcgtt tggaagctac tttttcttcc
      661 aactccattc gttttgattt ttgatctaac caagaggaat aattaccttt ccaaggaatt
      721 ccttctcctc tatctagttc taaaatccaa cccgctacat tgtcaaggaa atatctatcg
      781 tgcgttaccg caataacagt tccgggatat tgttgcaaat gttgctctaa ccaaagtaca
      841 gattcagcat ctaagtggtt ggttggctca tccaacaaca aaacatcagg ttgttgtaat
      901 aataaacgac acaaggcaac tcttcttttt tctccacctg ataacacttt tataggtgta
      961 tccgaatccg gtgtgcgaag tgcgtccatc gccacttcta atttattatc aatttcccaa
     1021 gcaccggctg catcaattct gtcttgcaat tctgcttgac gatccatcaa tttctgcatt
     1081 ttgtcggcat cttcatatac ctcaggtaaa ccgaacatat cgttgatttt attgaattca
     1141 tccaataaag cgatggtttc tgcagcacct tctctaacaa tttcaataac tgttttggtt
     1201 tcatccaatt gaggttcttg ttctaaataa cccactttat agcccggtgc aaaaaccaaa
     1261 tcgccttgaa aatttttatc gattccagcg atgattttta ataatgaaga ttttccggaa
     1321 ccatttaacc ctaaaatacc aatttttgct ccgtaaaaga aactcaaata gatatctttt
     1381 aaaacttgtt tattgttgct agagtaagtt ttactcactt tagacattga aaaaattact
     1441 tttttatcgt ctgccattgt ataaatattt ttctgaagtt aaaccctacc tttcaaagca
     1501 ttaaaaaccc aggcaatagc taaaaaacca attcctgtag aagcaaatcc ccaaccggct
     1561 acatcgtcat atttaaatgc tcccatacca attaaaagaa gtcccataat aatcataata
     1621 aaagtagccc aagccaatac ggtattttta ttcattccca tggttaatag ttttattttt
     1681 ggaagtgcaa atatcaacat attaaactaa tctaaaaaac gtttagttag ctttttgtta
     1741 aactttgcaa ggattacaaa attgaggttg gtttaaaatt ttcattttta gtatatttac
     1801 aaccttaaaa ataaacttta taaacatacc atggctttta ctccccttaa gtttaaatta
     1861 ctacttatct tttttatatt cacaggaata actttaatcg cacaggaaaa tcgtttaaaa
     1921 aacattcaaa agttaacttt tggtggtgac aatgccgaag cctattttag tccggatgga
     1981 aaaaaattaa cgcttcaagt gaccaacaaa gcttttggag ttccttgtga ccagattttc
     2041 atgcttgatt tacaagaaaa agaattttca gaaaaaagtt taaaactgat ttcaacagga
     2101 acaggtagaa ctacttgctc ctattannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnngaac tttggacaat ggatatcgat ggttctaatt taaaacaaat tacttttggt
     2281 ttaggatatg atggtggttg tttcttctct catgacagta agaaattagt ttttcgttca
     2341 tccagaccaa aaacagaaga agctattgct gaatacaaag ccttattagc tgagaattta
     2401 gtagctccct ctgatatgga aattcatacg tgtaatgttg acggttctga cttaaaacaa
     2461 atcacacatt tagggaaagc aaattgggct ccctattttc atccctcaga taagaaaatt
     2521 attttttctt ccaatcatca ttcaacaaga ggatatgact ttcaattgta tatgattgat
     2581 attgatggtg aaaatttgca acaaattacg tgggaaagtg aatttaacgc ctttccaatg
     2641 ttttcacctg atggaaaaaa attagttttt tcaagtaatc gtcaacagga aaaagcaaga
     2701 gaaacaaatg tttttatagc agattgggtt gatggtgatg tagctgaatt agctcaacca
     2761 aaatcattaa aaaagaatat ttcttattta gcctctgatg aattaaaagg aaggctgacc
     2821 ggttcgaaag aagaacaaat ggctgccgat tatttagtta aagaattaaa aagtctggga
     2881 atcaaaccct atgaaggaaa aaattatatt cagaaatttg actacaaagt gcgtttaaat
     2941 cctcatgact cgttagatct taaaaataat accggcagaa acgtcattgg ttttttagac
     3001 aataaagcaa ctaaaacaat tgtaataggt gctcattatg accatttagg tttaaatgaa
     3061 cataatcatt cagctaaacc aaattcaaaa ggtgaaattc acaatggtgc tgatgataat
     3121 gcatcaggtg ttgcggctgt tttagaattg gcccgtatgt tttctaaaaa taaaacaatt
     3181 gaaaaagtaa attacatttt tgctttcttt tcgggtgaag aagatggatt aattggttca
     3241 aaatttatga cttcattgtt gaaaaattat cattctaatg tggtttccat gattaattta
     3301 gacatgattg gacgcttgaa tgattctaaa aatctaactg taggtggtgt tggaactaac
     3361 cctaatttta aaacaatagt agataaaaat aaacctgccg gatttaatgt aacattggac
     3421 gaaagcggtg ttggaccttc tgaccatact tccttttatt taaaagatat tccggttttg
     3481 tttttcttca caggcacgca tactgattac cacaaaccta gtgatgatga ggttaaaatc
     3541 aattactatg gtgtaagaaa tattactgat tatgttttta gagttagtaa tgctattgct
     3601 gatttagaca aagttgaatt tgttaaaact aaaattgatg ctccaaaaac agctccaaaa
     3661 tataaagtta ctttaggaat tatgcctgat tatactgatc atggcgatgg tctacatgtt
     3721 gatggtgtaa cggataatcg tccggctcaa cttgccggaa ttcaaagcgg tgatatcatt
     3781 acaaaaattg gcgattgcga aatcaaagaa gtttacggct atatgaaatg tctttccggt
     3841 atcaatgccg gcgacgaatt acctgttaca ttcttacgaa atggagaaac aatgaccgca
     3901 aaggttaagt tttaatttaa ttaaaaatct tagctaacat ttctttcaac aaatcagatt
     3961 cgtgcaattg gttcgcacct actcctttac ttttgacatc aatgtcgcgt aaagtagaaa
     4021 caattgcact aacttttttc atcgggaagt ttttcaaagc caaatcataa tctttcaaaa
     4081 aatagggatt aacttttaaa actttggcaa cattaccggg ggatttatct tttaatccat
     4141 ggtattgtaa caagctggag aaaaagctaa agaccatccc ggttgtaaaa atcattggat
     4201 attccttctt attttgagca aaatgatcag caatgcgata ggcttgtaat tggtttcgtt
     4261 cgccaattgc ttttcgaaat tcgaaaacat taaaatcttt gctgaaacca atattttctt
     4321 caatgtgagt tggcgtaaaa gtggttcctt tcggtaaaat tatttggagt ttttccaatt
     4381 cattgttaat cctactcaaa tctgtgccca gaaactcaac taacatcgcg gtggctttcg
     4441 gttcaatatg atatcctttg ccttgtaaaa ctcttgaaat ccatgttcct atttgatttt
     4501 cgtacaattt tttactttca aaaagcaaac cttctttctc aattaattta gtcatacttt
     4561 ttcgcttgtc taagcttttg tacttgtacg caataactaa aaccgttgtt ggttgaaaat
     4621 ttttcacata actctccaat ttatcaatgt tacgtgacaa ttcctgagct tctcgaacaa
     4681 taattacctg acgatcggcc atcattggat aacgtttagc agctgaaata acatcttcaa
     4741 tagaaacatc tcttccgtat aaaacctgtt ggttaaatgc tctttcgtct tctgatagaa
     4801 tattactttc tataaattca gttaatttat caatataata aggctcttct cccatcaaaa
     4861 aatagatcgg tttgatgttt cctccactta tatctttgat aatttttgct atttcttcca
     4921 ttgattttaa taaggcataa atttgaatag aatttttact gaatcaattt tatatatttg
     4981 tacatgcaaa aactgaattt ttctgactat tcctttcgct tcaaaaatag tgaaaataaa
     5041 gtatccgtgt tcgatgaaat caggaaaaaa tttattgttc tcactccgga agaatgggtt
     5101 agaattcatg tggttcaata tttaattcaa gaaaaaaact atcctaaatc tttaataaac
     5161 gtcgaaaagc ttattaaaat taatacaata agtaaacgat atgatatagt tgtttatcaa
     5221 ccgaatggag aaatattttt gttgatagaa tgcaagaaac cggatgtaag tattaaccaa
     5281 ctcgtttttg atcaaattgc acgatacaac ttaactttga aggcaaaata tttaatggta
     5341 acaaacggac taaatcacta tttttgtcaa atggattttg agcaggaacg gtatgttttt
     5401 ttaaaggata ttcctgacta tgaacttcaa aactaatatt ttgcattcct ttattttatt
     5461 tatattttga agaaaatcgc aatcgttatt ctcaattgga atggggcaaa acttctacaa
     5521 cagtttctgc cttcagttct ttcctattct caggaagcaa caatctatgt agccgataat
     5581 gcctcaactg acgaatcgat ttctgtttta aaaacacaat ttccatcaat caagataata
     5641 aaaaacagct ctaattttgg ttttgcacaa ggttataatg aatgtttaaa atttgtagaa
     5701 gaagaaattt attgtttagt caattccgat attgaagtta cagaaaactg gttggctcca
     5761 atattggaac tatttgacaa aaacgaaaaa gtagccatta ttcaacccaa aattttagat
     5821 tttaaagaga aagcctattt tgaatatgcc ggtgcaggtg gaggttttat tgatagattt
     5881 ggttatccat tttgtcgagg tcgtattttc gattccattg aaaaagacaa cggacaatat
     5941 gatgatgtca aaatgatttt ttgggcttcc ggtgcctgct ttttcatccg gaaaaaagtt
     6001 tttagagaac tagaaggatt tgatgctgat ttttttgcac atcaggaaga aattgattta
     6061 tgctggagaa gtcataacct tcattatgaa acctattatt gtgggttttc aaaagtgtat
     6121 catttaggcg gaggaacgtt gtcgaataca aatccaagga aaactttttt aaattttaga
     6181 aattcgttgt ttatgttggt taaaaatttg ccaactgttc atctgatttg gattttattg
     6241 attcggatga ttttagatgg tattgccgga attcaattct tgtttcaagg aaaattttca
     6301 cattttacag caataattaa agctcatttt tatttctatg ctttgctggt aaaaatgttc
     6361 cgtaaaagaa aaaacaatca gcaatcaaat tattatttca ccacgagtat tatctaccat
     6421 tatttcatca aaaaagaaaa aatagttgtt gataaattta acaatagttt atcgtaatct
     6481 ggttaagatt ataagctttt tggtgttaat tttgtaataa atttaaaagg ataagttata
     6541 tgaaaaaagt cgttttatta ttagcactag ttgctctttc attaggttca tgtggcacga
     6601 agaaaaaaat tgccgaatta gagtctaaaa ataaagagtg tcaggattta ttaaattcta
     6661 caaccgtaaa gttaaacatg tgtttatctg aaaaagacat tttaaataat caaattgctg
     6721 atttaaaaaa gacaaattca gatttaatca gcaatgttgg aaatatgaca atgttatcca
     6781 ctaaaggagc agaaaactta gaaaaatcat tagagagttt aaaagaaaaa gatttaaaaa
     6841 tttctcgttt acaagatgct ttgaccaaaa aagatagtgt tactttagct ttagttacca
     6901 gtttaaaaag ttcggtaggt attaatgatc cggatataga aataaatgtt gaaaaaggag
     6961 ttgtcatgat ttctattgct gacaaaatgc tttttaaatc cggtagttat gaagttagtg
     7021 accgagctaa agaagtatta ggtaaagtag caaaagtcgt aaatgacaaa ccaacatttg
     7081 aatgcatggt tgagagtcat acagataatg ttccatacag aaatggagtt ttacttgaca
     7141 attgggattt gagtgttaaa agagctacct ctgttgttcg tgttttaact aacgatcata
     7201 aagtgagccc aaaacaatta atagctgccg gtagaggtga attcattcct ttagtagcaa
     7261 atgattctac acagaatcgt tcagcgaaca gaagaactcg tattatcgtt atgccaaaaa
     7321 ttgatgagtt ttatgatatg atagaaaaag aaatgaaaaa ccaagcgggt aaataagatt
     7381 ggttattctt caataaaaaa aacgtactga actttcagta cgtttttttt nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttttattatt cttttaaaaa atattgctat
     7561 caccttttcc ttcccggata acaaccggtt caccctctga taaatcaata attgttgaag
     7621 gctgattatc accataacca ccatcaataa ttaaatcaac tttattttgc catttctcaa
     7681 aaatcaattc aggatcagta gaatattcta acacttcgtc ttcatcataa atagaagttg
     7741 aaacaatagg attacctaaa agttttacaa tctctaatac aatagcatta ttaggaacgc
     7801 gaatgcccac agtcttcttc tttttaaatt cttttggaag attgttatta ccgggtaaaa
     7861 taaacgtata cggcccgggt aaagttcttt ttaaaatttt aaaagttgca gtatcaattt
     7921 gtctgatgta atccgaaata ttacttaaat catggcaaat aaaggaaaaa ttagcttttt
     7981 ctaatttaat tcctttgatt ttagcaattc gttctaaagc ttttgtgttg gtaatatcac
     8041 atcctaatcc gtatacggta tccgtaggat aaattactaa accaccttct ttgagcactt
     8101 ttacaacttt tgcaatggca gcttcattag gattttcagg atatattttg atgaattgag
     8161 acatttttta aattattgtt gagagcaaaa gtacattaga attttaaaac taccggtata
     8221 tttttaaaat taagcacaaa aaaagccctg caatgcaggg ctttaaatta ttttgcataa
     8281 aattattcag caactatttc ataaggcaat tcaactacaa catttctgtg taaacgcaca
     8341 gtagctgtat attttccggt tcttttaaca gttcctgaag tgatgaattt tctttcgatt
     8401 ggttgacctg ctttttctaa agcttcagcg atatcaatat tggtaacaga accaaataat
     8461 ttttcaccac cggcttttgc agaaatttta atatcaagag cttttaaggc ttcagctatt
     8521 tttttagcat catccactac tttttgctct ttgtgtgctt tttgtttcaa gttttcagcc
     8581 aatacttttt tagctgaagg agttgctaat gctgcaaacc cttgaggaat taaaaaattt
     8641 ctaccatatc ctggttttac agaaacgata tcgtctttaa accctaaatt ttgaacgtct
     8701 tgttttaaga ttagttccat gttgttgtcc ttttttatag aagttagccc gccaatggcg
     8761 aggcaacaac ttaattaatt attattttaa taaatccgcc acatatggca ttaaagctaa
     8821 atgtcttgct cttttaacag caacagacac ttttctttgg tattttaatg aagtacctgt
     8881 taatcgtctt ggtaaaattt taccttgctc gttaacaaat ttcaataaaa agtctgcatc
     8941 tttataatca atgtatttaa tacctgattt tttaaaacga caatactttt taattttatt
     9001 ggtatctatg ttcaaaggag taagatatct gatatctcct tctttttttc ctttagctga
     9061 ttgctcgatt gttgacatag taattacgct ttaactttta atttttctct tcttctctcc
     9121 gcccaagaaa ttgcatgttt gtctaaactt actgttagaa aacgcataat tctttcgtca
     9181 cgtcttaatt cagtttcaaa gctcaagatt gattccgcag gtgctttgaa ttcgaataaa
     9241 tggtaaaaac cacttttctt gtgttggatt tcataggcta attttttcaa gccccaatcc
     9301 tctttggata ccatctgtgc ccctttggcg gtaagaaaat cttcgaattt gcttactgtt
     9361 tcctttacct gttgttcaga taaaacggga ttcaagatga aaacagcttc ataatgattc
     9421 ataataattt actttaaaat tgttaaattg ggtgcaaaag taagaattta attttaatat
     9481 ccaagtctaa taagcaaata atcgacatag agcaaaaaag ttgaaaaaaa attagttttt
     9541 atcgatgaaa tgcactatgt ttgtttcacc aaatctataa taaccgtatt atgaaactaa
     9601 attgtgtcgt agtcgatgac agttctattc aaagaatgat catcacaaag ttagtgaata
     9661 atcaccagaa tcttaatcta gtaggtgatt tttctaatgc tattgaagca aagagttgta
     9721 tgtccataca ctccgttgac ctcatttttc ttgatatcga aatgcctata attaatgggt
     9781 tcgattttct tgatgggtta aaacaaaaac cccaaattat ttttatcacc tcaaaagcag
     9841 attacgctgt aaaagctttt gattatgatg caaccgatta tcttcaaaaa ccaatttctg
     9901 tagatcgttt tgattcttct gtaaaaagag ccatcgaatt gcataaatta agacatgaag
     9961 taacggaaga agaaggagat tatattttca taaaaagtaa tttaaaaaaa ttaaaagtat
    10021 tcacttctaa aattaaatgg attgaagcgt atggtgacta tgtgaaagta gttactgatg
    10081 aagacagcaa cttggtactt tctaccatga aatcgtttga aaatgattta tctaaagacc
    10141 gttttttacg agttcacaaa tcatttattg taaacattga taaaatcgaa cgtttcaata
    10201 gtaagtttgc tgaaattggc gtaaccaaaa ttccattaag cagaaataaa aaggatgatt
    10261 tgaaaaaagc tcttgctatt gcttaagtaa catcaacatt cacaacaagc tttactgctc
    10321 tatattgcga aataacatca aaactattca gtattttctg gatagttttt tttgtgcctt
    10381 gtggtgaaac cgtatccgga atcttaatca taatcgtccg aatgtattcg tttctaattt
    10441 tactgatagc aggttcttcc ggtcccaaaa ccggcatatt caattgttga tttaaaactt
    10501 gatataacca catagaactt tctttcactt tttcaaaatc tttatgtttt aaagtaattt
    10561 tgattaattt aaagaatggc gggtatttaa aaatctgtcg ttcgtataat tgttctttat
    10621 acattgccaa ataatcatgg tgagttacct gttgaatggt gttgtgattt ggattaaaag
    10681 tttgaatgat aactttccct tttttttcag accttcctgc cctgcctgaa acctgtgtca
    10741 tcatttgaaa acttctttca aaagctctga aatcaggaaa atatagcatg ttatctgcat
    10801 ttaatatccc aaccaaactt acattgtcaa aatctaaacc tttggctaac atttgtgtgc
    10861 ccactaaaat atctacttct ctattttgaa atttatctat gattttttca taaccaaatt
    10921 ttccacgggt tgtatcttga tccattcgtt gtatcttcct gtttgggaaa agagtggcta
    10981 attccaattc aatctgttcg gttccaaagc ctttagtagt caaatcaaca ctcgaacatt
    11041 gatgacaatt agttggttta gcaattgaat gtccgcaata atgacaacgc agttgatttt
    11101 tatatttatg atacgtcaaa cttacgtcac aattcggaca ttgcggaata tgtccacacg
    11161 tcatgcattc taacaaagga gcatatccac gtctgttttg aaaaagtata acctgttcat
    11221 ttaaagataa tgcggaagta atttcttcta ttaaagtgtc actaaaatga ccgttcattc
    11281 ttttacggaa aaacttatct ttcaaatcaa ctaaaataat ttctggcata ggaacattat
    11341 tatatcgttc aaaaagttct actaatccat atttatgaac ttgtgaatta taataggttt
    11401 ctatacttgg tgttgctgag cctaaaagaa ctttagcttt atgaaaagtg gccaaaacag
    11461 tcactgcatc gcgagcatga taacgtggag caggatcaga ttgtttaaaa ttttgttcat
    11521 gctcttcatc cactatcact aatcctaaat ttgaaaaagg caaaaacatg gccgagcgag
    11581 ctccgattac cacttgtgct tttggagaat tttctaaaac ctgattccac acttctaccc
    11641 tttcgttatt gctgtatttt gaatgaaaaa cggctacttt attcccgaaa aatttacgta
    11701 aacgtgaaag tagttgagtt gttaaagcta tttcaggaag taaaaacaat atttgcttat
    11761 cggttttcat catttcctga atgagttgac aatagatttc tgtttttcca ctggaagtta
    11821 ccccatgaag taaacaaatt tctttttgct caaattgtag tttaatttca tccaatgctt
    11881 tttgttgagc aggacttaaa actaagtttt catttaaatc taatgcatca aaatcggttc
    11941 tatcctggat aagatgatat tcttcaaaaa tagctttatc aacaagtact ttcaaaacag
    12001 ctgaggacac tttagaaaat tcgagcaatt gtttcgccga aataggcttt ttctcaacag
    12061 cttgcaattg aaaataacct aaaagtaact cactttgctt tttggctttt aatgaattta
    12121 acaactcatt caagctttca ttagaagtgt aattttgatg aagttttata tattttacta
    12181 atttaggttt ataattttcc tgaatttctt cctgtaacgt aatgatattt ttatcgatta
    12241 acttttgcaa aagaggaaaa atattttttt tatttaaaat agtcatcaca tcatttactt
    12301 tcaaagaact ttggtgttgg agagcttcaa aaattagaaa ttcatcatct gtgagattga
    12361 gttcctctac cgcttcatta tgtttcgaag aaataattgt ttcgctttcc aataataatg
    12421 ttgatggcaa tgcagcccga aacacttcgc caatcgaaca catataataa gtagaaatcc
    12481 attgccaatg ttttaactgg atttcattta caagaggatt ttcatctaaa atttgatgga
    12541 tttcttttgc ttcatataat gttggtgggt tttgatgcac ctcaattgcc aaagccgtaa
    12601 aaattttact tttaccaaag ggaacagcga ttctcattcc gggttttatg aaataatatt
    12661 ctgcctcagt tactttataa gtaaaagttt tatctagtgc taagggcaaa ataacttcaa
    12721 tgaagtaagc cataattgtt taattgcaaa aattagtagc agcaaaattc tgttttaatc
    12781 aaaaagattt tttctcttta atttattaat ggtagcattt agttcaaatc cgagtaataa
    12841 tatcatgcaa ttaatccaaa tgtaaaacat caggattaac aaggtgccaa tagaaccata
    12901 gagttcatta tatttggcaa acttgactac ataaattcca aaaacatagg atgaaagtaa
    12961 aattaaaagt gttgtaaaaa ctgaaccaat gcttataaat gaaacttttg atatttgttt
    13021 ggtaccaaat ttaaaaagta acgaggttgt tgataaaatc attaaaacaa caaatccaaa
    13081 ccgaatcatt tcaacaagtg taatatctgt gttgataatt ttatgtaaat gtattttttg
    13141 aataataatt tcagaaatta aaatggctac aatagttatt attaatatga atgaaaataa
    13201 aagtgacatt ccgagtgcaa taatatattg tctgatgaaa cttcggttta tggtaatatg
    13261 ataggaagtt tcaaatccgc ccaaaacggc attaatacca ttggtcatta aaaaaattga
    13321 caaaataaat ccggaagaca ataaaccttg gtaactgtta tttaggatgt catcgataat
    13381 tgttttgatt gcttcaaacg tattgggtgg cactccatct tctacaaatt tgagaaaatc
    13441 atcctgaaat ccttcgatag gaatataggg aatcaagttc aagatgaaca atgcaaacgg
    13501 aaataaggcc atgaaaaaac taaaagcaat tgctccggcc cgataagaaa aagccccttc
    13561 aacaattcca ataatatata attccatcaa atcataaaac gacaaacctt ctagccaagg
    13621 aagtttgatt cttttcatta atcgaactac gtatttgact attggtattt tatctatttt
    13681 ttcttctatc tctaatgaca ttttattgtt tcaagtttcg agttttatta aaaagctttt
    13741 aaacttaaat ccatgttata cacagaatgg gtcaatgctc ctgaagaaat ataattcacg
    13801 ccacaagctg catattgctg aatggttgtt tcattgatat tacctgaact ttcggttaag
    13861 catttatttc caattatttc tacagctttt ttagtggttt caaaatcaaa attatccagt
    13921 aatattctat aaacacctcc actactgaga atttcttcta cttccttgag gtttcttgct
    13981 tccacgataa tttttaagtc taactcattt tgtattagat aatctttcgt tttttgaata
    14041 gccaatgaaa ttccaccggc aaaatcgttg tgattatctt tcagcataat catatcataa
    14101 agagcaaaac gatggttttc gcctcctcct attttgacag cccatttttc agctgctcga
    14161 aatccgggag ttgtttttcg ggtatctaat atttttgtag ctgttccatc taataaatca
    14221 acatatttct ttgttttggt agctatagca ctcattcgtt gcattgaatt taatactaaa
    14281 cgttcggctt tcaaaattga ttgtgaactg ccggaaacat gaaaaacaac atctccatat
    14341 ttaaccaaac taccatcagg aataaacgtt tcaattttta aatttgagtc aacttcatta
    14401 aaaatcattt tagcaaattc ttcaccggct attattccac tgtcttttac taataatttt
    14461 gcttttcctt gttcagattt agggatacaa gccagcgaac tatggtctcc cgctccaata
    14521 tcttctctaa ttccattttt aattattaat tctaattcct tttgaaattg aacttcagta
    14581 atcatattat tgctttttta acgaaatgct aatgtaggaa atcgttttga tttttataga
    14641 atgattgatt caaaaaaacc acgctgttac gtggtttgat ttgaaatttt aaatagaggt
    14701 tatttttttt tcttcacttc acaagtactt ttgcccaaga gtgcataaag tgggcaaaat
    14761 gttgcaaaag cggtaataat taaaattgaa ctgacaccta aaactatcca tttgatcatg
    14821 tcgttttcaa taaaacccga cataaataca attaaaagta taattccgat gatgactcta
    14881 acaaaacggt ctgtattgcc tacatttttt ttcataataa aaagttttgg ttttttgtag
    14941 gttaaaatta tgaaaaaaaa agttttaatc cattaataat cgaatttagt ttggctctac
    15001 aatgagtcct ttccaatttg acattccacc aactaaatta aacgttttct caaaacccat
    15061 ttgtcgcatt atttcacaag cttttgcact tctacttccc gcatggcaat acacataata
    15121 acttttagct ttatctaatt catcgagcaa atatataaat ccttgccctt tataaatatc
    15181 tatattgatt gcattgggaa tgattccatg ttcaaattct tcgggagttc taacatctaa
    15241 gaaaactgcg ttttcatctt tttgagcctc tgcccaccat tgttcttgtt gtaaattcat
    15301 ttttttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccat aaaattaaaa
    15421 cttactctct ccttctaaaa atctttaaca aaaatttaat agacatttgt atatacaatt
    15481 aaaaaaatat catacatttg tacatacaaa ttttgtaaat acaaatgaaa atagaggaaa
    15541 tcataaaatc gaattctcta attagcaatg aaaagcgaac agttttaaac attatgttca
    15601 cccaaaacat tgttgcggat gcatttaatg agattttgaa aacttttgat ttatcggtag
    15661 aacaatttaa tgtattacgt attctacgcg gtcaaaaagg aaaaccggca aatatgtgtg
    15721 tcattcagga acgaatgatt gcaaaaacca gtaatacgac aaggcttgtc gataaattat
    15781 tattaaaagg tttagtgaat cgagtcgttt gtgagcaaaa cagacgaaaa atggaaatca
    15841 ctatcactga aagagggttg caacttttaa acgaattaga ccctaaaatt gaagcacatg
    15901 aattattatt tgccaacaat ctcaccaaac aagaattaga aaatttaaac gaattattag
    15961 aaaaatacag aacaatttaa ttatcaaact atgtcaaatt ttataactga tcaaaaatgg
    16021 cgttatgcca ctaagaaatt cgatgccagc aaaaaaatag ctgcatcaga tttagaaacg
    16081 ctaaaagaag ctattcaatt aagtacctct tcttatggtt tacaacccta taaaatattt
    16141 atcattgaaa atgctgaatt aagagcaaaa ctacaaccgg cagcatgggg acagtctcaa
    16201 attgtggatg cctcacatgt aatcgttttt gctaatatcg ttaattttgg tgatgctgaa
    16261 atagatgcct acataaacaa tttagcagaa acaaggggaa ttgaaataga aagtgtgaag
    16321 ggatacgctg attttataaa aatgaaaatc aatgctttat cagttgaaaa aagagatgtt
    16381 tggacttcaa agcaaactta tttagctttg gcaaatctta tgaatgcagc ggcagaactt
    16441 aaaattgatg taactcctat ggaaggtttt gaaccagaag cggtaaacga aataatggga
    16501 ttaaaaaaat taggtttaaa tgcttcttta attgccacaa taggatatag acataacgaa
    16561 gatttaaccc aacattacgc taaagtgcgt aaaccaaaaa gtgaactatt tatcaattta
    16621 taatcaataa ttaaaaacaa gaaaaacaat gaaaaatttt aaaacaatcg ctttagctct
    16681 tgttgttgcc ttaggaacat tttcagcaac agcacaaaac aaaaaaatta atgttgaaaa
    16741 aagctctatc aaatggattg gtaaaaaagt aaccggtcaa catgatggaa ctattaattt
    16801 tggatctgga accttagttt tcgaagggaa aaaattaaag ggaggttcat ttgaagtaaa
    16861 tatgacttca atcgctgttt ctgatttaga ggcaggaaaa ggaaaagaaa aattagaagg
    16921 tcatttaaaa tctggagact ttttcggtgt agaaaaatat gcaacatcaa cattagaatt
    16981 taaaaaaata ggttctaaag gaagtgatat ttatactgtg actgcagatt taaccattaa
    17041 aggaattaca gttcctgtta cttttgattt agctttagga gatggaactg ctagaacatc
    17101 tttcaaaatc aacagaacaa aatatgacat taaatatggt tcaggaagtt tctttgataa
    17161 tttaggagac aaagcaattg atgacgaatt tgaattaaat gtaaatttag tttactaata
    17221 aatagtggga aaaattgcga ataacctgac acgaaagtgt caggtttttt tatgtcttaa
    17281 tgataaaact ttgtattttt acctttctta accaaccaaa tgaaaaaagt attagtcata
    17341 gacaattacg atagttttac gtataattta gttcattatt tagaagattt gaattgtgaa
    17401 gtaacagttt ttagaaacga tgaatttgaa ctccatgaag tagagccttt tcagaaaata
    17461 cttctgtctc ccggtccggg tttgccaaag gatgccggat tattaaatca agttattaaa
    17521 aagtatgccc ctcaaaaaag tattttaggc atttgtttag gccttcaagc aattgctgaa
    17581 gtatttggtg gtaatttact taatttaatt aaagtacatc atggcgtttc ttctcaaata
    17641 actattattc aagaggatgt gcttttcgat ggtttaccta aaaacttaca agtaggaaga
    17701 tatcattctt gggtgataaa tacagaaaac attccggaag ttttagaagt tacctc
//
LOCUS       DAOTCW010000042        17515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1806_length_17515_cov_46.332016, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000042 DAOTCW010000000
VERSION     DAOTCW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 17515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17515
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1806_length_17515_cov_46.332016"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="P5335_05140"
     CDS             complement(<1..854)
                     /locus_tag="P5335_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRZ74299.1"
                     /translation="MKFLRLLLLFLSFSTFAQQGGMWIPSLLEGMNEKEMKSLGMKMS
                     AKDIYDVNKSSMKDAVPQFNGGCTSEVISSKGLLLTNHHCGYGQIQSHSTVENDYLEK
                     GFWAMNLAEELPNPGVVVTFIVKIEDITAKVLENTQSLSNEAEKQKKIQDNISALSNS
                     LPKENWQENKIRTFYDGNQYIWFVTETFKDIRLVGAPPSSIGKFGSDTDNWVWPRHTG
                     DFSLFRIYADKNNRPAEYSKDNVPYTPKHYFPVSISGVQENDFTMVFGYPGRTQEYLP
                     AVAVEQLV"
     gene            complement(877..1323)
                     /locus_tag="P5335_05145"
     CDS             complement(877..1323)
                     /locus_tag="P5335_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRZ74300.1"
                     /translation="MTTYNPISISDIENIIQLMGEFYRIDNYPFDIEISRKLFNQFLT
                     DENLGKAWLILYQNEIVGYVILTYVFSFEYQGKIAFLDELYIRENARGKGIGKETIAF
                     IQQEAAKLSLKIIYLEVETHNDAAQKLYIANDFAIHKRKIMKYIVN"
     gene            complement(1310..2551)
                     /locus_tag="P5335_05150"
     CDS             complement(1310..2551)
                     /locus_tag="P5335_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRZ74301.1"
                     /translation="MFFLYNILVHFAGFLLKISAVFNPKMKLFVEGRKNIFETLKHNI
                     STNDKTIWFHAASLGEFEQGLPVVEKIKIKFPSHKIIVTFFSPSGFEVRKNNSVADLT
                     IYLPLDTKENAKLFLELTHPELVFFIKYEYWPNYLNELKKQKTKTYLISGILRENQVF
                     FKWYGGFYRNCLQTFEHFFVQNESSKKLLQSLGYQNVKISGDTRFDRVVSILERDNSL
                     GFIEEFKKNTTTIVIGSSWPKDEELLVNFINQSSENVKFIIAPHNIKSEQIQELKKSI
                     SKSVILFSEKDSKKLNEYAVLIIDTIGILTKIYSYADIAYIGGGYGNPGVHNILEPAT
                     FGIPIVIGPNYTHFAEATALVGLEGCVSISSQNELNNAFSNLILNRDIRHEKGHICST
                     FVQMNKGATAVILKHIENDHL"
     gene            2705..>3824
                     /locus_tag="P5335_05155"
     CDS             2705..>3824
                     /locus_tag="P5335_05155"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRZ74302.1"
                     /translation="MRKLQMVDLKSQYDKIKTTVDASIQEVLDTTTYINGPKVHEFQK
                     NLEEYLDVKHVIPCANGTDALQIAMMGLGLQPGDEVITADFTFAATVEVIALLQLTPV
                     LVDVDLVNMNISLEGIRKAITPKTKAIVPVHLFGRAANMEAIMQIANEHNLYVIEDNA
                     QAIGANCRFSDGTKKKAGTIGHVGATSFFPSKNLGCYGDGGAIFTNDDELAHTIRGIV
                     NHGMYVRYHHDVVGVNSRLDSIQAAVLNTKLFLLDEYNAARQNAARKYSKAFEGHKNI
                     IAPTICDVCDCHVFHQYTLRIIDGDRNGLMDYLLSKGIPCAIYYPIPLHLQKAYIDSR
                     YKEEDFPITNQLVKEVISLPMHTELDDEQIKFITDSVLE"
     assembly_gap    3825..3924
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3925..4722
                     /gene="galE"
                     /locus_tag="P5335_05160"
     CDS             <3925..4722
                     /gene="galE"
                     /locus_tag="P5335_05160"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRZ74303.1"
                     /translation="QDVSGVIHFAASKAVGESVENPLLYYENNINTLVYILQELQQKP
                     EANFIFSSSCTVYGQAEKMPITEDASVQPAMSPYGNTKQIGEEIITDVTKVSSINAIL
                     LRYFNPIGAHPTAEIGELPIGIPQNLVPFITQTGIGLRKELSVYGDDYPTLDGTAIRD
                     YIHVVDLAKAHVVALQRLIEKKNLSKIETFNLGTGTGSSVLEVIHAFEKVSGQKLPYK
                     IVGRREGDITSAYANTDKANNVLGWKAQSTLEESLASAWKWEQKIRS"
     gene            complement(4796..5668)
                     /gene="fabD"
                     /locus_tag="P5335_05165"
     CDS             complement(4796..5668)
                     /gene="fabD"
                     /locus_tag="P5335_05165"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRZ74304.1"
                     /translation="MKAYVFPGQGAQFTGMGKELYETSALAKELFEKANEILGFKITD
                     IMFEGTPEELKETKVTQPAVFLHSVILAKTLTDFKPDMVAGHSLGEFSALVANGALSF
                     EDGLRLVFKRALAMQKACEITPSTMAAVLGLDDKIVEEVCEAIDGVVVAANYNCPGQL
                     VISGEYKAVELACEKMKEAGAKRALILPVGGAFHSPMMEPAREELAAAIEATTFSTPI
                     CPVYQNVTATAVSDAEEIKKNLIIQLTAPVRWTQSVNQMIADGATSFTEVGPGKVLVG
                     LVNKIKKEVETISA"
     gene            complement(5729..5953)
                     /locus_tag="P5335_05170"
     CDS             complement(5729..5953)
                     /locus_tag="P5335_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HRZ74305.1"
                     /translation="METENTKKEYTNGEITVVWEAGKCIHSGNCVRNNSAVFQPKEKP
                     WIKIDASSSEKIMETVNKCPSGALTFYRNK"
     gene            complement(5972..6256)
                     /locus_tag="P5335_05175"
     CDS             complement(5972..6256)
                     /locus_tag="P5335_05175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRZ74306.1"
                     /translation="MNEQIKLESNYKNGFFSVEIDGKIEAKMTFVFAGTDKIIIDHTE
                     VNPGNNGKGLGKKMVAAAVEMAREKNLKIIPLCPFAKSVFDKTLEFRDVL"
     gene            complement(6314..7027)
                     /locus_tag="P5335_05180"
     CDS             complement(6314..7027)
                     /locus_tag="P5335_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRZ74307.1"
                     /translation="MKNTVLHKANERGHADHGWLNAYHSFSFANWYNPDKVQFGVLRV
                     LNDDTIAPGMGFGTHPHDNMEIITIPLEGDLAHKDSMGNAEIIKFGDVQVMSAGTGIR
                     HSEFNPNEDKRTKLLQIWLFPNKRNVEPRYQQITLDVNDRQNKLQQILSPNPEDAGVW
                     IHQDAWFHMGKFEKGITKTYNVKRNENGVYAFVISGSITINGQVLETKDALGITNTEK
                     LEITATSDAEILLMDIPME"
     gene            7188..8153
                     /locus_tag="P5335_05185"
     CDS             7188..8153
                     /locus_tag="P5335_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRZ74308.1"
                     /translation="MSRDKKIRWGILGCGKIAEKFASDLELSENAVLVACASRIFEKS
                     KRFAKKYQATYFYDSYLELATCPDVDVIYIATPNSFHLEHSLLCLKNKKAVLCEKPLA
                     LNKFQVEEIIQVAKENNVFLMEAMWTSCLPNILQIQNTIKSGKIGEIIHLTADFGFKV
                     DYDPKSRLFDPTLGGGSLLDIGIYPLYISLLLMGKPAKIQSSLRYTKLGVDASCSVLL
                     SYTDQKTASLFSSFETFTAIKCEIYGTKGKIIISSNFHEQSQHTIIIDGLTGQLVQSE
                     LVGLGYFHEIEHVNECLYQGLNESYLMPLKMSMNLIELMDEIRSK"
     gene            complement(8171..8746)
                     /locus_tag="P5335_05190"
     CDS             complement(8171..8746)
                     /locus_tag="P5335_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRZ74309.1"
                     /translation="MSLLLNHIANYISLTEKEQKLLLSKTETVEYKAKTILLNAGEIC
                     KYSYFVNSGLLRSFNINDNIVEHVLHFACEGWWIGDMYSLLSQKPGNLFIEVLEDSEV
                     VLLSKENQELLYHEIPKLERFFRILTENSLVAHQERLMDNLSLSAEERFEKFCKRYPT
                     LIQRVPQKQIASYIGVTPEFFSKMKSKLLRK"
     gene            complement(8749..9768)
                     /locus_tag="P5335_05195"
     CDS             complement(8749..9768)
                     /locus_tag="P5335_05195"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013751122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HRZ74310.1"
                     /translation="MTIQEIIKNAPKAELHLHIEGSFEPELMFEIAQRNKVSIPYKNI
                     EEVKNAYKFDCLQDFLDIYYAGAGVLLHEIDFYDLTMAYFAKCHENNVIHTEIMFDPQ
                     THTHRGVPFETVITGIHKAQKVAEEKWGISSLLIMSYLRHLSEEDAFVTLEQSLPFKH
                     LIAAVGLDSSELGNPPSKFKNVFEASIKEGYIPLAHAGEEGPSDYVWEALDILNIHRI
                     DHGNNSLSDAKLIKRLVEENIALTVCPLSNSALKVVDDLKNHPLKTMLNHNLKATINS
                     DDPAYFGGYMNDNFLAIQEALDLSIADIQTLIRNGFEHSFSEKKVVFLEKTKAYFSPF
                     LSNLK"
     gene            complement(9804..>10639)
                     /gene="purT"
                     /locus_tag="P5335_05200"
     CDS             complement(9804..>10639)
                     /gene="purT"
                     /locus_tag="P5335_05200"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HRZ74311.1"
                     /translation="KELGLKTANYRYATSAEELQIAVSEVGIPCVVKPLMSSSGKGQS
                     TIKSKEDITKAWQYAVDGSRGDVVEVIVEAFVNFHSEITLLTVAQNNHLPTLFCAPIG
                     HRQERGDYQESWQPAKVSEKDILEAQEMARKVTKALGGAGLFGVEFFLTNEGVYFSEL
                     SPRPHDTGMVTLAGTQNFNEFELHLRAILSLPIYEITLEKAGASAVILAAENSNHPTF
                     SGIEKIAGLPKTDFRIFGKPTSRPYRRMGVSLVNDALETPIEEIVEKAKEAAKLVKVN
                     Q"
     assembly_gap    10640..10739
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10740..11886)
                     /locus_tag="P5335_05205"
     CDS             complement(<10740..11886)
                     /locus_tag="P5335_05205"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRZ74312.1"
                     /translation="MNPLSDRINNLSTSQTLAMAALARELKAQGKDIISLSLGEPDFN
                     TPDFIKEAAKKAIEENYSTYSPVDGYAELKEAICRKFKRDNNLDYKPANVVVSTGAKQ
                     SLYNIAQCMLNDGDEVILPAPYWVSYYEIIKMSGGIPVEVPTSVESDFKMTPEQLEKA
                     ITPKTKMIWYSSPCNPSGSVYSREEFTAIAKVLEKYPNIYIVADEIYEHINFSGTFCS
                     IASVPGMFERTITVNGVAKAFAMTGWRIGYIGAPEFIAKACTKMQGQVTSGANSIAQR
                     ATIAAVDADPKVLNYMVEAFHKRRDLVVGLIKEIPGLKINVPEGAFYVFPDVSSFFGK
                     TLRGTLIKDATDFSMYLLAEANVATVTGDAFGNPDCIRFSYATSEELL"
     gene            complement(11918..13012)
                     /locus_tag="P5335_05210"
     CDS             complement(11918..13012)
                     /locus_tag="P5335_05210"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRZ74313.1"
                     /translation="MNTTIPTFSKQDNLKFFRTLNSRVNNYFKDNNIQKTGNWKLHLK
                     TIVMFTIFLTPYFFLLTMDMPFWTYLLLNVVIGVGMAGVGMNVMHDGNHGSYSNKPWL
                     NKIMGGSIYILAGNVFNWQVQHNVLHHTYTNIVGHDEDLDAGRVIRFSKQSEWRYFHK
                     FQHYYSVFLYGLLTFNWAITTDFKQMRAYLKRKLSYGEPKSPKILWTTLIITKVIYFS
                     IWLVLPMVLGITWWKVVLGFAVMHYTAGLILSIVFQLAHVVEETTNPVPDENGEIENT
                     WAVHQLFTTANFAPKNWIVNWYTGGLNHQIEHHIFPNISHVHYGKISEIVKETAKECN
                     LPYHEFKTMRSAVIAHFKHLKELGQEPQMA"
     gene            13121..>13735
                     /gene="rsmG"
                     /locus_tag="P5335_05215"
     CDS             13121..>13735
                     /gene="rsmG"
                     /locus_tag="P5335_05215"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRZ74314.1"
                     /translation="MEEIIKYFPNLSENQLLRFKKLEELYHVWNAKINVISRKDIDEL
                     YIKHVLHSLGIAKVMEFQPKTTVLDVGTGGGFPGIPLAILFPETRFYLIDVIAKKIKV
                     VNEVVIALGLENVKAEQIRAENVKGDFDFIVSRAVTNMPDFVSWINDKIKKQQKHELK
                     NGVLYLKGGDLTEELKDFPKATQYNLSDYFSEDFFETKKVVHLPI"
     assembly_gap    13736..13835
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13836..15958)
                     /locus_tag="P5335_05220"
     CDS             complement(<13836..15958)
                     /locus_tag="P5335_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74315.1"
                     /translation="MKTKITLISLFTILFSFAQNGKYELLKDKTETNILFDQVFELSK
                     ITTEKKEIISSLYFKQVYHEIQRADYLQRLPKYDILKKEADNAFFSKQIPLALLISEV
                     ETIKTEVFENHQITINNREQLVLDPVVSDAFQQHKIVLMAPLVSKSKNKTVEFILKDK
                     NIFNTTSKTIQAIYMSFTDNDLWLTVSVNQPIKVDFSTYGKKSISFKIEFTNGESKVI
                     KSTFEIQGSSNLANRNGNEILAETITATIPFQGYGESAAYLGQGEYEIYLDNVDQVLD
                     KPIFLVDGFDPGDTRDTNLIYSLLNYGTSGENLGDIVRNEGFDIVVLNFPQYSPEDGV
                     IIDGGADFIQRNAMILVELINQINAQKVGNEQNVVIGPSMGGLISRYALRYMEQNQIE
                     HDTRLYLSFDSPHLGANVPIGFQHLFNYMAFGPLGDVTLQGVVNEVFKSPAAKQMLLD
                     HFEGHLQSGSLTEFNNSNQLPTGAPNFRTPFQNELTAMGFPTETRNIAISNGSGNGTM
                     TGTPGMFVLNDYTVDASSTQRAKFDVRFTPPTGVSNQLVSRFRAQQYILFWLTVFSST
                     ANAASPSTSSGLDSAPGGMFKVADFAAGGSGNPTLDDFLANLLIDKFCFIPTLSSLAI
                     TNPNWYTNVTGSSETPFVTTYVPTENEAHVTLTEGNVTFALNEILNPPLTIEQPDLLK
                     NITVKNPIHNSVEIFSPFTISNASIS"
     gene            complement(16062..>17515)
                     /gene="pruA"
                     /locus_tag="P5335_05225"
     CDS             complement(16062..>17515)
                     /gene="pruA"
                     /locus_tag="P5335_05225"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HRZ74316.1"
                     /translation="NMSAPHDHKHIVGTYHLAEKSHVEKAISNALESRTAWAAMPWEQ
                     RAAIFLKAAELIAGPYRAKINAATMIAQSKTIHQAEIDASCELIDFLRFNVQFMTQIY
                     SDQPSSDSSVWNRVEYRPLEGFVYAITPFNFTAIAANLPASAAMMGNVVVWKPSDSQV
                     FSAKIIIDVFKEAGVPDGVINVVFGDPVMISDTIFEHPSFAGIHYTGSTFVFKEIWKK
                     IGTNIHKYKTYPRIVGETGGKDFIIAHPSANVKQVVANSIRGAFEFQGQKCSAASRAY
                     YPKSLWPAIKEEMAKEMATIKMGSPEDFSNFFTAVIHEGSFDKLAKYIDQAKSDADAE
                     ILFGGNYDKSKGYFVEPTVILTTNPHYVTIETELFGPVLTVYIYDDANWKETLKLVDQ
                     TSEYALTGAVFSQDRYAITEATQALENCAGNFYVNDKPTGAVVGMQPFGGARASGTND
                     KAGSAQNLLRWVSPRTIKETFVTPENYRYPFLG"
BASE COUNT         5500 a         2939 c         2942 g         5834 t
ORIGIN      
        1 tttacaagtt gttccacggc aacggcaggc aaatattcct gcgttcttcc cggataacca
       61 aaaaccatgg taaaatcatt ttcttgaaca ccacttatgg aaacaggaaa ataatgttta
      121 ggagtgtaag gaacattatc ttttgaatac tctgccgggc ggttgttttt gtcggcataa
      181 attctaaata aagaaaaatc tcctgtatga cgtggccaaa cccaattgtc tgtatctgaa
      241 ccaaattttc caatcgatga aggtggagca ccaactaatc gaatatcttt aaaagtttcg
      301 gtcacaaacc atatgtattg attgccatcg taaaatgttc tgattttatt ttcttgccaa
      361 ttttctttag gtaaagaatt gcttagagca gaaatattat cctgaatttt tttctgtttt
      421 tctgcttcgt tggacaagga ttgtgtgttt tctaaaacct tggccgtaat gtcttcaatt
      481 ttcacaataa aagtgactac tactcccgga ttcggtaatt cctccgccaa attcatggcc
      541 caaaatcctt tttccaaata gtcattttct acggttgaat gggattgaat ttgaccatat
      601 ccacaatgat gattcgttaa tagtaatcct ttggatgaaa ttacttctga tgtacaacct
      661 ccattgaatt gaggaacagc atctttcata ctggatttgt taacatcata aatgtcttta
      721 gctgacattt tcatgccaag tgatttcatt tctttttcgt tcataccttc taataaagag
      781 ggaatccaca tgcccccttg ttgagcaaaa gtggaaaaag aaagaaataa taggagtaag
      841 cgtaaaaatt tcataggact aaatttatgt attcgattag tttacaatat atttcatgat
      901 ttttcgcttg tgaatggcga aatcgtttgc aatatacaat ttttgagccg catcattatg
      961 tgtttctacc tccaaataga tgatttttaa cgataattta gcagcttctt gttgaataaa
     1021 ggcaattgtt tcttttccaa ttcctttgcc acgagcgttt tctctgatgt ataattcatc
     1081 taaaaaggca attttacctt ggtattcaaa actgaaaaca taggttagaa ttacataacc
     1141 aacaatttca ttttgatata aaattagcca agcttttccc agattttcgt ctgtaaggaa
     1201 ttgattaaag agttttctgg aaatttcgat gtcaaatggg taattgtcaa tgcgatagaa
     1261 ttcgcccatc aattgaatga tgttttcaat atcggaaata gagattggat tataagtggt
     1321 cattttcaat atgcttcaaa ataacggcag ttgctccttt attcatttgt acaaaagtgc
     1381 tacaaatatg tcctttttca tgacgaatgt cacgattcaa aattaaattt gagaaggcat
     1441 tatttaactc attttgactt gaaattgaaa cacagccttc taagcccact aaagctgttg
     1501 cttcagcaaa atgagtataa tttggtccga ttacaattgg aataccaaaa gtggccggtt
     1561 ccaaaatatt gtgaacaccc gggtttccat atcctccgcc aatatatgca atatcagcgt
     1621 aactatagat tttggttaag atgccaatgg tgtctataat taaaacagca tattcgttta
     1681 attttttact gtctttttca gaaaataaaa ttacactttt ggagattgat ttttttaatt
     1741 cttgaatttg ttccgatttt atattatgag gagcaataat gaattttacg ttttcagatg
     1801 attgattgat gaaattcacc aataattctt catcttttgg ccatgaactt ccaataacta
     1861 ttgtagttgt gtttttttta aattcttcaa taaaacccaa agaattgtct ctttctaaaa
     1921 tcgaaacaac gcgatcaaaa cgggtatcgc cggaaatttt gacattttga taacccagcg
     1981 attgcaataa tttttttgag gattcatttt gtacaaaaaa atgctcaaat gtttggagac
     2041 aatttctgta aaatccacca taccatttaa aaaacacttg attttctctt agaattcctg
     2101 aaattaaata ggttttagtc ttttgttttt taagttcatt caaatagttt ggccaatatt
     2161 catatttaat aaaaaacact aattcaggat gagttaattc taaaaatagt tttgcatttt
     2221 cctttgtatc taaaggcaaa taaatagtga ggtcagcaac agaattgttt tttctgactt
     2281 caaatccgga aggagaaaaa aaggtgacaa ttattttatg agaaggaaat ttgattttga
     2341 ttttttccac caccggaaga ccttgctcaa actcgcctaa tgaggcagcg tgaaaccaaa
     2401 ttgtcttgtc attcgtggaa atattatgtt tcagggtttc aaaaatattt tttctccctt
     2461 ctacaaaaag tttcattttc ggattaaaaa cagcactgat ttttaataag aatcctgcga
     2521 aatgaaccaa tatattatat aaaaaaaaca tagactaata attgtgtcgc taaaatacat
     2581 cactttgtag aattttcatt gttataactt cttaaataat tacttttgtt ctattattta
     2641 aggtgtaaaa tgtgccatgc cttttaaacc ttaaactttt aaactttaaa caaaagaaaa
     2701 agtgatgcgt aaacttcaaa tggttgactt aaaaagtcaa tacgataaaa ttaaaaccac
     2761 cgttgatgct tcaattcaag aagttttgga tacaacaacc tatattaacg gtcctaaggt
     2821 tcacgaattt caaaaaaatt tagaagaata tttagacgta aagcatgtaa ttccttgtgc
     2881 aaacggaacc gatgcacttc aaattgccat gatgggattg ggtttgcaac ccggtgatga
     2941 agtaataacg gccgatttta cttttgcagc aaccgtggaa gtgattgcgt tattacaatt
     3001 aacacctgtt ttggttgatg ttgacttagt gaatatgaat atatcgcttg agggaattag
     3061 aaaagcaatt actccaaaaa caaaagcaat tgttcctgtt catttgtttg gaagagccgc
     3121 caatatggaa gccattatgc aaattgcaaa tgaacataat ttatatgtta ttgaagataa
     3181 tgctcaggcg attggagcca attgtcgttt ttctgatgga actaaaaaga aagccggaac
     3241 gattggtcat gttggagcta cctctttttt cccatcaaaa aatttaggtt gttatggtga
     3301 tggaggagca atttttacaa acgatgatga gttagctcat accatacgag gaattgttaa
     3361 tcatgggatg tatgttcgat atcatcatga tgtagtaggt gtaaattcaa gattagatag
     3421 tattcaagcg gcagtattga acacaaaatt atttttatta gacgagtata atgccgcacg
     3481 tcaaaatgct gcacgaaaat attcaaaagc atttgaaggg cataaaaata ttattgcacc
     3541 aactatttgt gatgtttgtg attgtcatgt ttttcatcaa tatacgttga gaattattga
     3601 cggcgaccgt aatggtttaa tggattattt acttagcaaa ggaataccgt gtgctattta
     3661 ttatccaatt cccttgcatt tgcaaaaagc ttatatcgat tcgcgatata aagaagaaga
     3721 ttttccaatc accaatcaat tagtgaaaga agtaatttca ctgccaatgc atacagaatt
     3781 agacgacgaa caaattaaat ttataactga ttctgtttta gaatnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnncaggat gtttcaggtg tcattcattt tgcggcttca
     3961 aaagctgtag gtgaaagcgt agagaatcca ttattgtatt atgaaaacaa cattaatacg
     4021 ttagtttata ttttacaaga attacaacaa aaaccggaag caaattttat cttcagttca
     4081 tcttgtactg tttacggtca agccgaaaaa atgccaatca cagaagatgc ctcggttcaa
     4141 ccggcaatgt ctccttatgg aaatacaaaa caaataggag aagaaattat aacggatgtt
     4201 accaaagtat cttcaattaa cgctatttta ttgcgttatt tcaatcctat aggagctcat
     4261 ccaacggcag aaataggtga attaccaatt ggtattccac aaaatttagt gccgtttatt
     4321 acgcaaacag gaattggttt acgtaaagaa ttatccgttt atggggatga ttatccaact
     4381 cttgatggca cggcaattcg cgattatatt catgtagtgg atttggctaa agctcacgtg
     4441 gttgctttac aacgattaat tgaaaaaaag aatttatcta aaatagaaac ctttaattta
     4501 ggaacgggaa caggaagttc ggttttagaa gttattcatg cttttgaaaa agtatccggc
     4561 caaaaattac cttataaaat tgttggcaga agagaagggg atatcacttc agcttatgct
     4621 aatactgata aagctaataa tgtgttaggt tggaaagcac aatcaacatt ggaagaaagt
     4681 ttagcgagtg catggaaatg ggaacaaaaa attagaagtt agcttaaaaa tttcaatata
     4741 aaaagggaca aatttaattc aaaatttgtc ccttttttgt gatttatttt tctgtttaag
     4801 ccgaaatcgt ttctacttct ttcttgattt tatttactaa tccaactaat actttcccag
     4861 gaccaacttc cgtaaaacta gtcgccccat cagcaatcat ttgattcaca gattgcgtcc
     4921 atcgaaccgg agccgttaac tgaataatca aattcttttt aatttcttct gcatcagaaa
     4981 ccgcagtagc tgttacattt tgatagactg gacaaatagg agtagagaat gtcgttgctt
     5041 caatcgcagc ggctaattct tctctggctg gttccatcat cggagaatga aaagcaccac
     5101 caacaggtaa aattaaagca cgttttgcac ccgcttcttt cattttttca caagccaatt
     5161 caactgcctt atattctcct gaaataacta attgcccagg gcaattataa tttgcggcaa
     5221 caacaacgcc atcaatagct tcgcaaactt cttctacaat tttgtcatct aaacctaaaa
     5281 cagcggccat cgtagaaggt gtaatttcac atgctttttg catggctaaa gctcgtttga
     5341 aaactaatcg caaaccatct tcaaacgata atgctccgtt agcaaccaat gcagaaaact
     5401 cacccaacga atgaccggca accatgtccg gtttaaaatc agttagcgtt ttagctaaaa
     5461 taaccgagtg taaaaaaacg gcaggttgcg tcactttggt ttcttttaat tcttcaggag
     5521 ttccttcaaa catgatatcg gttattttaa aacctagaat ttcatttgct ttttcaaata
     5581 attctttagc taatgcggaa gtttcatata gttctttgcc cattcctgtg aactgagctc
     5641 cttgtcccgg aaatacgtat gctttcattt ttcatttaat ttttacagca tttattacta
     5701 ctgatgatta ttttaaaaaa caaaaatact atttatttct ataaaatgtt aaagcacctg
     5761 acggacattt gttaactgtt tccatgattt tttcagaaga ggatgcatca attttaatcc
     5821 aaggtttttc tttgggttga aaaacagcag agttgtttcg gacacaattt ccggaatgta
     5881 tacattttcc tgcctcccaa acgaccgtta tttcaccgtt ggtatactct tttttggtgt
     5941 tttcagtttc catggttttt ttgtttattg gttacaaaac atctctaaat tccaaagttt
     6001 tgtcaaaaac acttttagca aaaggacaaa gcggaattat ttttaagttt ttttcccgag
     6061 ccatttcaac agcagcggcc accatttttt tccccaatcc ttttccgtta tttcccggat
     6121 taacttctgt atgatcaatg ataatcttgt ctgtgccagc aaaaacaaaa gtcattttag
     6181 cctctatttt tccatcaatt tctacagaaa agaaaccatt tttgtagttt gattctagtt
     6241 taatttgctc attcatggca tgttggtttt aaacctgtca ggtaaaaata acgtgatagg
     6301 taaagttaat aaattattcc attggaatat ccatcaacaa aatttctgca tcggaagttg
     6361 ctgtaatctc caatttctca gtatttgtaa ttcccaatgc atctttggtt tccaaaactt
     6421 gtccgttaat agttatgctt cctgaaatca caaaagcata tacaccattt tcatttcttt
     6481 tgacattata agttttggtt attccttttt caaattttcc catgtgaaac catgcatctt
     6541 gatgtatcca aacgcctgca tcttctggat ttggggataa aatttgttgt aatttgtttt
     6601 gtctgtcgtt tacgtccaaa gtgatttgtt ggtaacgagg ttccacgttt cgtttgtttg
     6661 gaaacaacca aatttgtagc agtttagttc ttttatcttc attcggatta aattcactgt
     6721 gtctaattcc ggttccggca ctcattactt gaacatcacc aaatttgata atttcagcat
     6781 ttcccatgct gtctttgtga gctagatcac cttctaatgg aattgtaatg atttccatat
     6841 tatcatgtgg atgggtgcca aaacccatac caggtgcaat agtatcgtcg tttaacaccc
     6901 gtaaaactcc aaattgaact ttatccggat tgtaccaatt ggcaaaacta aaactatgat
     6961 aagcattcag ccaaccatga tcggcatgtc ctctttcatt ggctttgtgt aaaactgtat
     7021 ttttcataac taaaagaatt tattattatt tatgaaacaa atttacgatt gatagttcta
     7081 agtaattctt aatttagatt aagaaatatc cgtttgactt ttaatgttta ttagtttttg
     7141 gaatcaatag tataaattgt ctaattttat ttaaaataga ggatatgatg agtagagaca
     7201 agaaaatacg atggggaatt ttaggttgcg gaaaaattgc ggaaaaattt gcaagtgatt
     7261 tagaattgag tgaaaacgca gtccttgtag cttgtgcatc aagaattttt gaaaaaagta
     7321 aacgttttgc aaagaaatac caggctactt atttttatga ttcgtatttg gaactagcta
     7381 cttgtccgga tgttgatgta atctatattg cgacacctaa ttcatttcac ttggagcact
     7441 ctttattgtg tttaaaaaat aaaaaagcag ttctatgtga aaagccactc gctttaaata
     7501 aatttcaagt ggaagagatt attcaagtag caaaagaaaa taatgtgttt ttaatggaag
     7561 caatgtggac gagttgcttg ccaaatattt tacaaattca aaacaccatc aagagtggta
     7621 aaataggaga aattattcat cttacggctg attttggttt taaagttgat tatgatccaa
     7681 aaagtagact ttttgaccca actttgggag gaggttcgtt acttgatatt ggaatttatc
     7741 cattgtatat cagccttctt ttaatgggta aaccagctaa aattcagtct tctttacgtt
     7801 atacaaaatt gggggtagac gcttcctgtt ctgtcttgct ttcttataca gatcaaaaaa
     7861 cagctagttt attctcttct tttgaaacat ttaccgctat taaatgtgaa atttatggta
     7921 cgaaagggaa aattattatt tcatctaatt ttcatgaaca aagtcagcac acaattataa
     7981 tagatggatt aacaggtcaa ttggtacaaa gtgaactagt tggactgggt tattttcatg
     8041 aaattgaaca tgtgaacgaa tgcctatatc aaggtttgaa tgaaagttat ttgatgcctt
     8101 taaaaatgtc aatgaattta atagaactga tggatgaaat cagaagtaaa taaatcaata
     8161 atctgtcaat ttatttacgc agcaatttac ttttcatttt actgaaaaat tcaggtgtta
     8221 caccaatata agaagcgatt tgtttttgag gaaccctttg gattaaggtt gggtatcttt
     8281 tacagaattt ttcaaaacgc tcttcggcgg ataaacttaa attatccatc aaccgttctt
     8341 gatgagcaac taaggaattt tcagttaaaa ttctaaaaaa gcgttccaat tttgggattt
     8401 catgatacaa taactcttga ttttctttgg ataataaaac tacttctgaa tcctctaaaa
     8461 cttcaataaa caaattgccg ggtttttgag aaagcaagct gtacatgtct ccaatccacc
     8521 aaccttcgca ggcaaaatgc aaaacatgtt cgacaatgtt atcatttata ttgaaactgc
     8581 gtaacaatcc tgaatttacg aaataggaat atttacatat ttcaccggca tttaaaagga
     8641 tggtttttgc tttgtactca acagtttcag ttttagataa caaaagcttt tgttcttttt
     8701 cagtaaggga aatatagtta gcaatatggt ttaaaagcaa tgacatgctt attttagatt
     8761 gcttaaaaaa gggctaaaat acgctttcgt tttttctaaa aaaacaactt ttttttcaga
     8821 gaatgaatgt tcaaatccgt tccgaattaa cgtttgaata tcggcaatag ataaatccaa
     8881 agcctcttga atagctaaga aattatcatt catgtagcct ccaaaatagg ctggatcatc
     8941 tgagtttatt gtcgctttta aattatgatt tagcatggtt tttaagggat gattttttaa
     9001 atcatctaca acttttaaag ctgaatttga aagagggcat accgttagag caatgttttc
     9061 ttctactaat cgtttgatta gttttgcgtc agataaacta ttgtttccgt ggtcgattct
     9121 gtgtatgtta agaatgtcta atgcttccca aacataatca gaaggacctt cttctccagc
     9181 atgagctaat ggaatataac cttcttttat agaagcttca aaaacatttt taaatttaga
     9241 aggaggattg cctaactctg aagaatctaa tcctaccgca gcaatcaaat gtttaaatgg
     9301 taatgattgt tctagggtta caaatgcatc ttcttcagag agatgcctca aataagacat
     9361 tattaaaagg gaagaaatac cccatttttc ttcagccact ttttgtgctt tatgaattcc
     9421 tgtgataacc gtttcaaatg gtactccacg atgcgtatgt gtttgtgggt caaacataat
     9481 ttcagtatgt atcacattat tctcatgaca ttttgcaaaa taagccatcg tcaaatcata
     9541 gaagtctatt tcgtgtaaaa gaactcctgc accggcataa tagatatcta gaaaatcttg
     9601 tagacagtca aatttgtagg catttttgac ttcttcaata tttttatagg gaatggaaac
     9661 tttatttcgt tgagcaattt caaacattaa ttcaggttca aacgaacctt caatgtgtaa
     9721 atgaagctct gctttaggag catttttgat aatttcttgt attgtcattg taagtataaa
     9781 taaataaagc aagatactat tttctattga tttactttaa ccaatttagc tgcttctttt
     9841 gccttctcga caatttcttc aattggtgtt tccaacgcat cattcaccaa tgaaactccc
     9901 atcctacgat agggtcttga tgtaggtttg ccaaaaattc tgaaatctgt tttgggtaat
     9961 cctgcaattt tttcaattcc ggaaaaggtt ggatgatttg aattttcagc cgctaaaata
    10021 actgcacttg ctcctgcttt ttctaaagtg atttcataga ttggtaaact taaaatagct
    10081 cgtaaatgca attcaaattc gttaaaattt tgtgttccgg ctaacgtaac cataccagta
    10141 tcgtgtggtc ttggtgataa ttcggagaaa taaacacctt cgttggttag aaaaaattct
    10201 acgccaaata aaccagctcc acctaaagct tttgtcactt ttcgagccat ttcttgagct
    10261 tctaaaatat ctttttcaga tacttttgcc ggttgccaac tttcttggta atcgccgcgt
    10321 tcttgccggt gaccaattgg agcacaaaat aaagttggta aatggttgtt ttgagcaact
    10381 gttaataggg taatttccga gtgaaaattc acaaaggctt ctacaattac ttctaccaca
    10441 tcaccacgag agccatccac agcatattgc caagctttag taatatcttc ctttgatttt
    10501 atggtggatt gtccttttcc ggatgaactc atcaatggtt tgactacaca tggaatacca
    10561 acttctgaaa ccgctatttg caattcctct gcggaagttg cataacgata attcgcagtc
    10621 tttagtccta actctttggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    10741 gagtaattct tcgcttgttg catacgaaaa tcgaatgcaa tccggatttc caaaagcatc
    10801 tccggttaca gttgcaacat tagcttcagc taacaaatac atagagaaat ctgtcgcgtc
    10861 tttgattaaa gtaccacgta atgtttttcc aaagaaggaa gatacatccg gaaatacata
    10921 aaaagcacct tcaggaacat tgatttttag acccggaatt tctttgatta atcctacgac
    10981 taaatctctt cttttgtgaa aagcttccac catgtaattc aatactttcg ggtcggcatc
    11041 cacagcggca atagtagctc tttgagctat tgaattggct ccagaggtta cctgaccttg
    11101 cattttggta caagctttcg caatgaattc aggtgctcca atatatccga ttctccatcc
    11161 tgtcatggca aatgcttttg caactccatt tacagttatg gttctttcaa acattcccgg
    11221 aacagaagca atactgcaaa aagttcctga gaaattgata tgttcgtaaa tttcgtcggc
    11281 aacaatatat atgttggggt atttttctaa tactttagca atagctgtaa attcttcacg
    11341 gctgtaaaca gaaccacttg ggttacatgg cgaactatac caaatcattt ttgtttttgg
    11401 tgtgatggct ttttcaagtt gttcaggagt cattttgaaa tcgctttcga cagaagtagg
    11461 aacttcgaca ggaattccac ccgacatttt gataatttca taataactta cccagtaagg
    11521 agccggtaaa attacttcat caccatcatt caacatgcat tgtgctatgt tgtaaaggga
    11581 ttgtttggca ccagtggaaa ccacaacatt tgccggttta taatccagat tattgtctct
    11641 tttgaatttt cgacagatag cttcttttaa ttcagcataa ccatctaccg gtgaataggt
    11701 gctataattt tcctcaattg ctttttttgc tgcttcttta ataaagtcag gtgtgttgaa
    11761 atcaggttct cctaaactta aactgatgat gtctttaccc tgagctttta actctcttgc
    11821 caatgctgcc atagccaagg tttgagaagt agataaatta ttaatcctat ccgataacgg
    11881 gttcattgta gtatgttttg tagttgtgta aattttatta tgccatttgt ggctcttgac
    11941 ctaattcttt caaatgttta aagtgagcaa taacagcact tcgcatcgtt ttgaattcgt
    12001 ggtaaggaag attgcattct ttggcagttt ctttcacaat ttcactgatt ttaccatagt
    12061 gaacgtgact aatattaggg aaaatatggt gttcaatctg atgatttaat ccaccggtgt
    12121 accaattcac aatccaattt tttggtgcaa aattagcagt tgtaaataat tggtgaacag
    12181 cccatgtatt ttcaatttcg ccattttcat ctggcactgg atttgtagtt tcttccacaa
    12241 cgtgagccaa ttggaaaaca atgcttaaaa ttaatccggc agtatagtgc attacagcaa
    12301 atccaagaac tactttccac caagtgatgc ctaataccat tggtaatacc aaccatattg
    12361 agaaataaat aactttggtg ataattaaag ttgtccaaag aatttttgga cttttaggtt
    12421 cgccataaga taatttacgt ttcaaataag ctctcatttg tttgaaatca gttgtaattg
    12481 cccaattgaa agttaataaa ccatatagaa aaacagaata gtagtgttga aatttatgaa
    12541 aatatctcca ttctgattgt tttgaaaaac gaataacacg tcctgcatct aaatcttcat
    12601 catgaccaac aatgttagtg taagtgtgat gcaaaacatt gtgttgcact tgccagttga
    12661 aaacgtttcc ggctaaaatg tagatacttc cacccattat tttattcaac caaggtttgt
    12721 ttgaataaga accatgatta ccatcatgca ttacattcat tcccacaccg gccattccca
    12781 caccaatcac tacatttaat agtaaatagg tccaaaaagg catgtccata gttaatagga
    12841 aaaaataagg agttaagaat atagtaaaca tcactattgt ttttaagtga agtttccaat
    12901 tacctgtttt ttgaatatta ttatctttga aatagttgtt aacgcgactg ttaagggttc
    12961 taaaaaattt tagattgtct tgttttgaaa aagttggaat tgtggtattc atgtatttta
    13021 taaattttcc ccaaaggtaa gtattaatat aaatcataac gatgaacaaa caataaaatt
    13081 tcaacaccaa attgtttact tttgttaaaa atagcacaat atggaagaaa ttataaagta
    13141 ttttcctaat ctatctgaaa atcagcttct tcgatttaaa aaattggaag aattatacca
    13201 cgtttggaat gcaaaaatca atgttatctc ccgaaaagac attgatgaac tatacattaa
    13261 acatgtttta cattctctgg gaattgctaa agtgatggaa tttcaaccca aaacaacagt
    13321 tttagatgtt ggaaccggtg gtggttttcc cggaattccg ttggcaattt tgtttccgga
    13381 gacacgattt tatttaatag acgtgattgc taagaaaata aaagttgtga atgaagtagt
    13441 gattgcatta ggattagaaa atgtcaaagc ggaacagatt cgtgctgaaa atgtcaaagg
    13501 ggattttgat ttcatagtga gtagagccgt cacgaatatg cctgattttg tctcttggat
    13561 aaacgataaa atcaagaagc agcaaaaaca cgaactaaaa aatggtgttt tatatcttaa
    13621 aggaggagat ttgacggagg aattaaaaga ttttccaaaa gcaacacaat ataatttgtc
    13681 ggattacttc tctgaagatt ttttcgagac caagaaagtg gttcatttac caatannnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngatat cgaagcattt gaaatagtaa
    13861 acggactgaa aatttccaca ctattatgta taggattttt gacagttatg tttttcaata
    13921 aatcaggttg ttctatagtt aaaggcggat ttagaatttc atttaaagcg aatgtaacat
    13981 ttccttctgt taaggttacg tgagcttcat tttcagttgg aacataagtg gtcacaaatg
    14041 gtgtttctga acttccggtg acattcgtat accaatttgg attagttatg gctaatgaac
    14101 tcaatgttgg gataaaacaa aatttgtcaa ttaataaatt cgctaaaaaa tcatctaaag
    14161 tgggatttcc acttcctccg gcagcaaaat cagcgacttt aaacattccg ccgggagcag
    14221 agtctaaacc ggaagaagtt gaaggcgaag cggcattagc ggtacttgaa aaaacagtta
    14281 accaaaataa aatatattgc tgtgctctaa acctacttac taattgattt gaaacacctg
    14341 taggcggagt aaaacgaaca tcaaattttg ctcgttgtgt tgaactagca tcaacggtat
    14401 aatcatttaa gacaaacata ccaggagttc cggtcattgt cccgtttccg ctcccattcg
    14461 aaatagcaat atttctcgtt tccgttggga aacccattgc agttaactca ttttgaaatg
    14521 gagttctgaa atttggagca ccggtaggaa gttggtttga gttattaaat tccgttaaac
    14581 taccactttg aagatgtcct tcaaaatgat ccaaaagcat ttgtttggca gccggacttt
    14641 tgaaaacttc atttacaaca ccttgtaatg ttacatcacc aagcgggcca aaagccatat
    14701 aattaaataa atgttgaaaa ccaataggta catttgcccc taaatgaggg gagtcaaaag
    14761 aaagatataa gcgtgtatcg tgttcaattt gattttgttc catataacgc aaagcataac
    14821 gggaaatcaa gccacccata cttgggccaa tcactacatt ttgttcatta cctacttttt
    14881 gagcattgat ttgattgatg agttccacta aaatcatcgc atttcgttga ataaaatcgg
    14941 caccaccatc tataattact ccatcctcag gtgaatattg aggaaaattc aaaacgacaa
    15001 tatcaaatcc ttcgtttcgg actatgtcac ctaagttttc tccggaagtg ccataattta
    15061 gcaaggaata gatgaggttt gtgtctcttg tgtcgcctgg gtcaaatcca tctacaagaa
    15121 aaataggttt gtctaaaact tggtcaacgt tgtctaaata aatttcatat tcgccttgac
    15181 ctaaataagc ggcactttct ccatatcctt gaaaaggaat tgttgccgta attgtttcag
    15241 ctaaaatttc gtttccgttt cgattagcca aatttgaact tccttgtatt tcaaatgttg
    15301 atttgataac tttactttct ccgtttgtaa attcaatttt aaatgaaatt gattttttgc
    15361 cgtaagtgct aaaatcgact ttaatgggtt gatttacaga aacagttaac caaagatcat
    15421 tatcggtaaa gctcatgtaa attgcctgaa tagttttgga agttgtgttg aaaatatttt
    15481 tgtctttcag aataaattca actgttttgt tttttgattt ggaaactaac ggagccatca
    15541 atacaatttt atgttgttga aaagcatctg agaccactgg gtctaaaaca agttgttccc
    15601 gattatttat tgtaatttga tggttctcaa aaacttccgt tttgatcgtt tcaacttctg
    15661 aaattaaaag tgctaaagga atttgttttg aaaaaaaggc attatcggcc tcctttttta
    15721 aaatgtcata tttaggtaaa cgttgtaaat aatcagctct ttgaatttcg tgataaacct
    15781 gtttgaaata tagagatgaa ataatttctt ttttttcagt ggtgatttta gaaagttcaa
    15841 aaacttgatc aaataagatg tttgtttctg ttttgtcttt taatagttcg tattttccat
    15901 tttgagcaaa tgaaaataaa atagtaaaaa gggatataag agtaattttt gttttcatta
    15961 gccaatggtt tgttttgata gtgaatggtt tacttctttc aaatataaaa aaaagtccga
    16021 acaaacagct cggacttcat gaaatattat ttttaaatcg attaacctaa aaacggataa
    16081 cgataatttt caggagttac gaaggtttct ttgattgttc ttggcgaaac ccaacgcaat
    16141 aaattttgag cagaaccagc tttgtcattg gttccggaag cacgagcacc accaaaaggt
    16201 tgcattccta ctacagctcc ggttggttta tcattcacat aaaagttacc ggcacagttt
    16261 tctaaagcct gagttgcttc agttatagca tatctgtctt ggctaaaaac tgctccagtt
    16321 aaagcgtatt ctgaagtttg atcaactaat tttaaagttt ctttccaatt agcatcgtca
    16381 taaatgtaaa cagttaaaac agggccaaat aattcagttt caatagttac ataatgtgga
    16441 tttgttgtta atatgactgt aggctcaaca aagtaaccct ttgatttgtc ataattacca
    16501 ccaaataaaa tctcagcatc agcatcgctt tttgcttggt cgatgtattt tgccaatttg
    16561 tcgaatgatc cttcatgaat tacagcggtg aagaaattag aaaaatcttc aggggaaccc
    16621 attttaattg tggccatttc tttcgccatt tcttccttga tagccggcca taaacttttc
    16681 ggatagtaag cccttgaggc tgccgaacat ttttgacctt ggaattcaaa agcacctcga
    16741 atagaattag ctacaacttg tttcacattt gcacttggat gagctatgat aaaatctttt
    16801 ccgcctgttt caccaacaat tcgtggataa gttttgtatt tatgaatgtt ggttccaatt
    16861 ttcttccaaa tttccttaaa aacaaaagta gaaccggtat aatgaattcc tgcaaaagat
    16921 gggtgttcaa aaatggtatc tgaaatcatt accgggtctc caaaaacgac attgataaca
    16981 ccatctggaa cgccagcttc tttgaaaaca tcaataataa tcttggctga aaatacttgg
    17041 ctgtcacttg gtttccaaac tacaacatta cccatcatgg ctgcagaagc gggaaggtta
    17101 gctgcaattg cggtaaaatt aaatggtgta atggcataga caaaaccctc taacggacgg
    17161 tattccactc gattccaaac agaagaatca gaagatggtt ggtcactgta aatttgggtc
    17221 atgaattgta cattgaaacg caaaaaatca attaattcgc aagaagcatc aatttctgcc
    17281 tgatgaatag tttttgattg agcaatcata gttgcggcat ttatttttgc acgatatgga
    17341 ccggcaatca gttcagccgc ttttaagaaa atggcagcac gttgttccca aggcatcgca
    17401 gcccatgctg ttctggattc caatgcattt gaaatagctt tttctacatg acttttttct
    17461 gctaaatgat aagtcccaac gatgtgttta tggtcgtgtg gagcactcat gttcc
//
LOCUS       DAOTCW010000043        17322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1833_length_17322_cov_45.417154, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000043 DAOTCW010000000
VERSION     DAOTCW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 17322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17322
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1833_length_17322_cov_45.417154"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3370)
                     /locus_tag="P5335_05230"
     CDS             complement(<1..>3370)
                     /locus_tag="P5335_05230"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRZ74317.1"
                     /translation="AIYLKNSHFTMIDENSEDPKILEFLKINAQLDGFKIKGTDVTTA
                     ITKMSFMDFRGLKVENLKTQFTYTKKNILLQKLKLLTKNSLLNADVALRYEREDFSDF
                     NNKVVFDIKVDTASVATNDIGYFYKELGKNNKFNFTASILGTLNDFHTNQLNLVDLNN
                     TKIIGDINFKNLFGDEKQGFFLKSKFDKLTSNHDDLAALLPNILGKSLPSSLKKLGQF
                     TIFGNADITTKSIKTDFFMTSSLGNLETDLVMSNINNIDNATYKGNVILENFNVGLLL
                     NRKEFGLVSMNSDVDGKGFTLKLIDTKFNGKISKFEYNNYTYTGILVNGNFKKPIFKG
                     KVNVNDPNLFMDFDGIVDVSKKENVYKFDAKIDYANLSKLKLVKNDSISIFKGKIYSN
                     LVGNSVNNLYGDVLIEETSYQNQKDIYFFDNFKLSSSFDQKRERTITINSPDIIEGKV
                     VGKFDFEQVPKIFENALGSIYTNYQPNTVRKGQYLKFNFALYNKIIEIFYPDISLSSN
                     TYIRGSMNSDTNDFKVDFNSPKIKVYENVINNLNLEIDNKNPLFNTYVTIDSLNTKLY
                     KISDFNLINITRKDTLFFRSEFKGGKDATDYYNIDAYHTIDNKKNNIIGLMKSELKFK
                     DYLWFLNEENNESNRIVFDKNFTNFEFDNFSLTNDIQKINFLGTITNKDDKDLELNFT
                     QVDIGKIIPEIENFVMAGNLNGKIKFKQKNNLIEPLAAISIDELRVNNIPLGNLALNV
                     TGNNEMNSFKVNSTLENKNMESFKLDGNLSYLNGENVMDMDLKLNKFNIGAFSTIGGE
                     VISNIRGLVSGNANFQGSLSNPEMNGRLFLSKAGLTVPYLNVDYELEENAIVDLTERQ
                     FLLRNINLTDSKYKTKGVLFGNVRHSKLKDWKLDINVNTDYMVVLDTEESEDAYYYGK
                     AFISGRASIIGPTTALLIDVNAKSEKGTFIKLPISDEQGVGEKNFIHFITKEEKFNLS
                     SNLNRPIRDYTGLELQFDLDINENAEIEVILDKETGHNMKGRGVGNLLLQINTLGKFN
                     MYGDFMVYQGEYNFKYRGLVSKKFKVKKNGNIVWEGDPLRARLNLEAVYETRANPAVL
                     MNNSSVNQKVPVEVAILINGTISNPDPDFNINLPT"
     assembly_gap    3371..3470
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3471..3928)
                     /locus_tag="P5335_05235"
     CDS             complement(<3471..3928)
                     /locus_tag="P5335_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74318.1"
                     /translation="MVFLLLVLILGILLSLPVVQTKIGQYITEQINKDYGTDINVEQV
                     ALTVFGGVKLKKVLIKDHHKDTLFYINRLKTNILDFNKLYNGDLLFGDIRIDGLIFDL
                     KQYKNEKETNLDKFVSAFDDGSPSTGNFFMKADAIYLKNSHFTMIDENSE"
     gene            4111..5133
                     /gene="tsaD"
                     /locus_tag="P5335_05240"
     CDS             4111..5133
                     /gene="tsaD"
                     /locus_tag="P5335_05240"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRZ74319.1"
                     /translation="MTLDPIFILAIESSCDDTAAAVLKNDKVLSNVVANQRIHEEFGG
                     VVPELASRAHQQNIVPVVDLALKKAGITKKQLHGIAFTQGPGLMGSLLVGSSFAKSFS
                     MALGIPLLAVNHMQAHILAHFIDEEGFDKPTFPFLALTISGGHTQIVKVNHFFDMEII
                     GETTDDAVGEAFDKTAKILGLPYPGGPLIDQFAKEGNPKAFGFTKPKVEGLNFSFSGL
                     KTQILYFVQKNTALNPNFIEENKADICASVQFTIIQILMEKLKLAVNQTGINQIAIGG
                     GVSANSGIRKTLKEAEAKYGWKTYIPKFEYTTDNAAMIGIVGYQKYLHNQFEKASVVS
                     KARIEF"
     gene            5133..5837
                     /locus_tag="P5335_05245"
     CDS             5133..5837
                     /locus_tag="P5335_05245"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRZ74320.1"
                     /translation="MQLFYFQHIDKDSKQFSFDKEESKHINKVLRKKEGDILFVTNGL
                     GFIFKTEINLASDSKCTVSIISFEQQVKSKIHLHLVVAPTKMNDRYEWFLEKATEIGV
                     QEITPIICEHSERKVIKSERFDKILLSAMKQSNQYFLPILNESITLKEFLKKEIVGQK
                     FIAHCEETNKKSLKDCLQKGENCTILIGPEGDFSSKEIQLALENQYIPISLGNTRLRT
                     ETAAVVACHSVVFENE"
     gene            5847..6488
                     /locus_tag="P5335_05250"
     CDS             5847..6488
                     /locus_tag="P5335_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HRZ74321.1"
                     /translation="MKNIFLAFLILIGFQTFAQEIALLKYNGGGDWYANPTSLPNLIK
                     FCNQNISTNLKNKPATVEPCSPDLLSYPFVHMTGHGNVVFNDNEILNLRNYLTSGGFI
                     HIDDNFGMDKYIRKEIKKIFPNSDLVEIPATHAIFQKPYPFPNGLPKIHEHENQKPQA
                     FGIFIEGRLVLFYTFECDLGDGWEDEEVHNDPKEVREKALKMGANLVHYVFTN"
     gene            6531..7619
                     /locus_tag="P5335_05255"
     CDS             6531..7619
                     /locus_tag="P5335_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRZ74322.1"
                     /translation="MTSKIIADGILKAIGKLTLIMLVLFFIYAIRTILIYLIVALILT
                     LILNPIVEFFKNKCKFNNLWAVITTFTILVVAVSLFILMFIPLISSQANSLSLLDTNA
                     IQEKITLLYYDVSLFLIDRGILMEEILKDFDINSILNLSILPSFFNGLLSTISSIGIG
                     IASVFFITFFFLKDKVLFIIGAKQIIPDSHEEETLNSIHKTYYLLSRYFIGLLLQLFI
                     VFILYYIVLLIFGIHNALIIALLCAILNIIPYIGPTIASFLAAILSMIGHIGEDFQTV
                     MLPSTIYILIAFFIVQLIDNNVNQPLIFSKSTKSHPLEIFLVILIAGFLTGILGMIIA
                     VPLYTIIKVIAKEFYPNNKFVKILTKNL"
     gene            7631..8818
                     /locus_tag="P5335_05260"
     CDS             7631..8818
                     /locus_tag="P5335_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRZ74323.1"
                     /translation="MEKFKQLLTEEVQQFINDRIGKSVSDLAFQKNPFPQVDWKLMLN
                     QIEAKSKAKDKLPTWFSKSKIIFPSKVAVEQTSSEQTAQYKSSLIVGNSLIDLTGGFG
                     VDTFYFSKRFSKVNHCERNNELSEIVKHNFSQLKVNNINCISGDSELILSDLNQKFDW
                     IFIDPSRRNDAKGKVFILSDCEPNVPELLDFYYKFSSSILIKTAPLLDLSAGLKELKN
                     VATIHIIALENEVKELLWEIKQNHSDFPIIKTVNITKKGIEKFEFKKFTDTQKPLFSE
                     PEKYLYEPNTAVMKSGGFDEVSSQFDIKKLHQHSHLYTSDKEITFCGRVFEIQKQLSY
                     NKIEMKLFLENQKANVTVRNFPETVENIRKKWKIKDGGELFCFFTTDLNNNKIVLLCK
                     KLK"
     gene            8824..9387
                     /locus_tag="P5335_05265"
     CDS             8824..9387
                     /locus_tag="P5335_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74324.1"
                     /translation="MKLIIFSLILLFSLQITAQKKCDYTVEVNDSLGTLKTTKEILMF
                     ERRFGNNENYIFFSLSLDNGTPVLNFQQVQKNNEFIKAFCIDASTKLYLQLENGKIIT
                     LVHTNTENCGTFFRNEDKNVRVLSANFLFLKNTIEELKASPVNLMRVKYSTETVDYLI
                     KPEMQSELLNEFIRPENYFIDYLHCVE"
     gene            complement(9389..10033)
                     /locus_tag="P5335_05270"
     CDS             complement(9389..10033)
                     /locus_tag="P5335_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRZ74325.1"
                     /translation="MNKILFFLIFLLFSVLGYSQEKIPKQNEQHTIQDTTFVNLKDYS
                     TDIVYDMRYATTSNFLKEKVYDCAECYLRYKTVKKLIEANQKFIVLGFRIKLYDCYRP
                     LAIQKKMWKIVPNPIYVADPSKGSIHNRGGAVDITLVDKNGLELEMGTTFDYFGKEAS
                     HNYTDLPEKVLQNRKLLKEIMESCSFKAFQSEWWHYHLISDELDPISNFKWNCN"
     gene            complement(10069..10539)
                     /locus_tag="P5335_05275"
     CDS             complement(10069..10539)
                     /locus_tag="P5335_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74326.1"
                     /translation="MNIINRLNALELQQKKNLDELGALRKYLEENPKLVASWQDLNEL
                     SGYYIDSFSKIEAYENRPINDENRNVFVTENQAKATLAKAQLSQLLKSFHSGWNHHLA
                     NYAIFPQIKDGQFSAYIGISILPQFLAFRSREKAQLFYQQHHELIHQYWAEFLD"
     gene            10985..>11849
                     /locus_tag="P5335_05280"
     CDS             10985..>11849
                     /locus_tag="P5335_05280"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HRZ74327.1"
                     /translation="MKKITNLIFLCSLLIVSVVSAQNARLQVIHNSPDLAAALVDVYV
                     NDDLLLNDFAFRTATPFIDVPAGVELSIDIAPSTSTSSAESLYNLTATLTADETYIVV
                     ANGIVSPSGYSPAQPFALNVFAQGREVASNSANTDVLVNHGSPDAPTVDVVETSVPAG
                     TIVNDISFPDFAGYLELPNLDFTLDVRDATGTVTVASYQAPLQTLGLDGAAITVLASG
                     FLDPAVNSNGPAFGLWVALASGGNLIPLPLVETPTARLQVIHNSPDLAAALVDVYVND
                     DLLLNDFAFRTA"
     assembly_gap    11850..11949
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11950..12281
                     /locus_tag="P5335_05285"
     CDS             <11950..12281
                     /locus_tag="P5335_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ74328.1"
                     /translation="VNSNGPAFGLWVALASGGELIPLPQIPLSVTDFNSSKISIYPNP
                     TSSQLNLDIPFDYENFKGIIYDLSGREVKTFSNPNSIYVSDLNNGLYILNVTLDEVNF
                     NQKFLKN"
     gene            complement(12420..12493)
                     /locus_tag="P5335_05290"
     tRNA            complement(12420..12493)
                     /locus_tag="P5335_05290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12457..12459),aa:Arg,seq:tct)
     gene            complement(12499..12573)
                     /locus_tag="P5335_05295"
     tRNA            complement(12499..12573)
                     /locus_tag="P5335_05295"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12536..12538),aa:Pro,seq:tgg)
     gene            complement(12582..12665)
                     /locus_tag="P5335_05300"
     tRNA            complement(12582..12665)
                     /locus_tag="P5335_05300"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12631..12633),aa:Ser,seq:gct)
     gene            12960..14144
                     /locus_tag="P5335_05305"
     CDS             12960..14144
                     /locus_tag="P5335_05305"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRZ74329.1"
                     /translation="MNNTKIVIVSAVRTPIGSFMGGLSTVPAPQLGAIAIQGALAKIK
                     LDPKLIDEVFMGNVVQAGVGQAPARQAAILAGLPNTVACTTINKVCASGMKALMLGAQ
                     AIKSGDAEIVVVGGMENMSLIPHYVHLRNGYKFGPASMIDGMQKDGLVDAYDNNAMGV
                     CADLCASEYKISREAQDNFAIQSYERSAKAWSEGKFDNEIVPVAVPQRRGEPIIISKD
                     EEFTNVKLDKIPSLNAVFTKDGTVTAANASTINDGAAAMIIMSEEKANSLGLKPLAYV
                     RGYADAAQEPKWFTTAPAKALPKALDKAGISISEVDFFEFNEAFAVVGLANSQLLGLN
                     ESKINVNGGAVSLGHPLGCSGARIIVTLLNVLQQNNSKIGAAAICNGGGGASAVVIER
                     AS"
     gene            14167..14928
                     /locus_tag="P5335_05310"
     CDS             14167..14928
                     /locus_tag="P5335_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRZ74330.1"
                     /translation="MYGICNLAIIPLRIEPNDRSEQVSQVLFGEHFEILEQLKQWFKI
                     RLEFDGYQGWIDSKQCQIISEMDFNEISGSLQILNNDLVEYITSDSNLLMPVPLGASL
                     TFLNYEGINKYNYEFEGMKATGVKPKSELLKTAFMYLNAPYLWGGKTPFGIDCSGFTQ
                     MVYKLNGYKLLRDASQQATQGEALSFIEESEPGDLAFFDNEEGKIIHVGIIMHDNYII
                     HASGKVRIDRLDHLGIYNADTNRHTHKLRVIKKII"
     gene            complement(14988..16274)
                     /locus_tag="P5335_05315"
     CDS             complement(14988..16274)
                     /locus_tag="P5335_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74331.1"
                     /translation="MKSRYIIPAFALMLSLSTMAQKDQLKALEKATKNYETTAVKNTL
                     AAAEAVIATAPDAEKAHFYFLKGNALLELSNHNVESGNNLILAAKSYQDLIALEKSMG
                     KSKYTSQAEASLATVRDNLVNSAIADNKADKYKEGTEKLYQAYRISPKDTVYLYYAAN
                     GAIRTKDYDTAIEYLKQLKDLNYTGKATEYFATDKATKAETSFGTKAERDLYIKAGTH
                     EKPREEKVPSVRGEVYRNYSILLAEKGQVDKALLEIQAARKLYPNDMDFVLIEADFYN
                     RKGDVENYVRIIKEASDKNPKDADLVYNLGVAYTKMNNEVEATKYFKKTIEMKPDYVN
                     AYINLANFKIDEQSRIFEEMNSLGNSSAENKKYNELMDKKNKLLEEAIPYLETALTYK
                     PNNLDILNLMTQIYPTLGMDDKYKETKAKIESIKAQ"
     gene            complement(16298..>17322)
                     /locus_tag="P5335_05320"
     CDS             complement(16298..>17322)
                     /locus_tag="P5335_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRZ74332.1"
                     /translation="NLSEYKTQNRGGVGQKSAGTRDQDFLEHLFVATNHQYMLFFTQK
                     GKCFWMRVYEIPEGSKTSKGRAIQNLINIENDDKVKAFICTQDLKNEEYINSHYVIMA
                     TKQGQVKKTLLEQYSRPRQNGIAAITIKEDDELLEAKLTNGKSQILVAVKSGKLVRFE
                     ESKTRPMGRNASGVRGITLADDKDEVIGMVSVNDMSSEILVVSENGYGKRSSLEDYRI
                     TNRGGKGVKTLNITDKTGRLVSINSVTDADDLMIINKSGLTIRMEVSDLRVMGRATQG
                     VRLINIKGNDSIAAVTKVMKEEAVEEVEFEDVTAAVLNSDSKIETTDFDDDSDETTEE
                     NEETQE"
BASE COUNT         5747 a         2749 c         2481 g         6145 t
ORIGIN      
        1 gtgggcaagt taatattaaa atcagggtca ggattactta tagttccatt aataagaatt
       61 gcaacttcca caggaacctt ttgatttaca gaagaattat tcatcaaaac agccggatta
      121 gctcttgttt cataaactgc ctcaagattc aaacgggctc taagtgggtc accttcccaa
      181 acaatatttc cgttcttttt aactttaaat tttttactga ccaatcctct gtatttgaaa
      241 ttatattccc cttgataaac cataaaatct ccatacatat tgaattttcc gagcgtgtta
      301 atttgcaaaa gtaaatttcc tacacctctt cctttcatgt tgtgaccggt ctctttatct
      361 aatataactt caatttctgc attttcattt atatctaaat caaattgcag ctctaatcct
      421 gtgtaatcac gaatgggtct atttaaatta ctgcttaaat taaatttttc ttcttttgtg
      481 atgaaatgaa taaaattctt ttcacctaca ccttgctcat ctgatattgg taatttaatg
      541 aacgttcctt tttcagattt tgcattaaca tcaattaaga gagctgttgt tggtcctatt
      601 atacttgctc ttcctgaaat gaatgctttc ccataatagt aagcatcttc gctttcctcc
      661 gtatcaagta caaccatata atcggtatta acattgatat ctaatttcca atcttttaat
      721 ttggaatgtc tgacatttcc aaacaaaact cctttggttt tatattttga atccgtaaga
      781 ttgatatttc ttaataaaaa ttgtctttca gttaaatcaa caatggcatt ctcttctaat
      841 tcataatcaa catttaaata cggaacggtt aaaccggctt tactcaaaaa caaacgaccg
      901 ttcatttccg gatttgacaa acttccttga aaatttgcat ttcctgatac taaacctctg
      961 atgtttgata taacttcacc cccaattgta ctgaatgctc cgatattaaa tttgtttaac
     1021 tttaaatcca tatccatcac attctcacca tttaaatagg acaaattacc atccaattta
     1081 aagctttcca tatttttatt ttctaatgta gaattaactt taaacgaatt catttcatta
     1141 ttaccggtca cattcaaagc taaatttcct aaaggaatat tgtttactcg caattcatca
     1201 atactaatag ctgccaatgg ttcaattaaa ttgttttttt gtttgaattt aattttacca
     1261 ttcagatttc ctgccatcac aaaattttcg atttcaggaa taattttacc tatgtcaact
     1321 tgtgtaaagt ttagctctaa atctttatcg tctttattcg taattgtacc taaaaagtta
     1381 attttctgaa tatcatttgt aagcgagaaa ttatcaaatt cgaaatttgt aaaattctta
     1441 tcaaaaacta ttctattcga ttcattattc tcctcattta aaaaccataa ataatcttta
     1501 aatttcagtt ctgatttcat caatccaata atattatttt tcttattatc aatggtgtga
     1561 taggcatcta tattataata atccgttgca tctttcccac ctttaaattc tgaacggaaa
     1621 aaaagcgtgt cttttcgggt aatattaatt aaattgaaat cagaaatttt atataatttg
     1681 gtattcaaac tatcaatagt gacataagta ttaaaaagtg gatttttatt atcaatttct
     1741 aaattcaaat tattaattac attttcgtaa accttaattt ttggtgagtt aaaatcaact
     1801 ttgaaatcat tcgtgtcgga attcatactt cctcttatat atgtatttga actgagtgaa
     1861 atatcaggat aaaagatttc gataatcttg ttatataacg caaagttaaa ttttaaatat
     1921 tgcccttttc ttaccgtatt aggttgataa ttagtataaa tactacctaa ggcattttca
     1981 aaaatcttag gaacttgttc aaaatcaaac ttaccaacaa ctttaccttc aataatatcc
     2041 ggtgaattaa tggtgatggt tctttctctt ttttgatcaa atgatgaact caatttaaaa
     2101 ttatcaaaaa agtaaatatc tttttgattt tgataagatg tttcttcaat taaaacatcg
     2161 ccatacaagt tattaacgct gttacctact aaattggaat aaattttacc tttaaaaatt
     2221 gaaatactgt cattcttaac gagttttaat ttactcaaat tagcataatc aattttagca
     2281 tcaaatttgt agacattttc tttttttgat acatcaacaa ttccatcaaa atccataaat
     2341 aaatttggat cattgacatt cactttacct ttaaaaatag gctttttaaa gtttccgtta
     2401 actaaaattc ccgtataagt ataattatta tattcaaatt tactgatctt accattgaat
     2461 tttgtatcaa ttaattttaa tgtaaaacct tttccatcaa catcagaatt catagaaact
     2521 aatccaaact cttttctatt taacaataat cccacattaa aattctctaa aatcacatta
     2581 cctttatagg ttgcgttatc aatattattg atatttgaca tgactaaatc tgtttctaaa
     2641 ttacccaatg aagaagtcat aaaaaaatca gttttaattg attttgtggt tatatcagca
     2701 ttaccgaaaa ttgtaaactg tcctaatttt ttcaatgaac ttggcaatga tttccccaag
     2761 atattaggca ataaagcggc taaatcatca tgatttgaag taagtttgtc gaatttgctt
     2821 tttaagaaaa aaccttgttt ctcatcacca aataagtttt taaaatttat atcaccaata
     2881 attttagtgt tatttaaatc tactaaattt aactgattag tatgaaaatc atttaaagtt
     2941 cctaagattg aagccgtaaa attgaattta ttgttttttc ctaattcttt gtagaaatac
     3001 ccgatatcat ttgttgcaac agaagctgta tctactttta tatcaaatac tactttatta
     3061 ttaaaatctg aaaaatcttc tctttcatag cgaagagcaa catcagcatt taacaatgaa
     3121 tttttagtta aaagtttcag tttttgaagt aaaatatttt ttttggtata cgtaaattga
     3181 gtttttaagt tttcgacttt caatcctctg aaatccataa aagacatttt tgtaatagcg
     3241 gtagttacat ctgttccttt tattttaaat ccatctaatt gggcattaat ttttaaaaat
     3301 tccaatatct ttggatcttc actattctca tcaatcattg taaaatgact gtttttcaaa
     3361 taaatagcat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcttcactat
     3481 tctcatcaat cattgtaaaa tgactgtttt tcaaataaat agcatctgct ttcataaaaa
     3541 aatttccggt agaaggagaa ccatcatcaa aagcacttac aaatttatcc agattagttt
     3601 ctttttcgtt tttatattgt tttaagtcaa aaattaatcc atcgattcta atatctccaa
     3661 ataataaatc accgttgtac agtttattaa aatctaatat gttagttttt aatcgattaa
     3721 tataaaaaag cgtgtcttta tggtgatctt taatcaaaac cttcttaagt ttaactccac
     3781 caaaaacggt caaagcaact tgctcaacat taatatcagt accataatct ttattgattt
     3841 gttcagtaat atattgtcca attttagttt gaaccacggg taacgacaaa agaataccga
     3901 gtatcagcac taatagcaga aaaaccacta aaaatcgtaa tactattttt ttaaattttt
     3961 tgatacccct atttgttata aatttgtaac gtttattgta aagttataat tttacaacaa
     4021 cacaaaaata aataatttta acataagaaa tttactttct tttaagctaa attattggaa
     4081 acaaatatag ttcaaaattc gtgcctcttt atgacacttg atcccatttt tatattagca
     4141 attgaaagtt cctgtgatga tacagctgct gctgtgttga aaaatgacaa agtattatcc
     4201 aatgttgttg ccaatcagcg aattcatgag gaatttggcg gtgtagtacc tgaattggcc
     4261 tcaagagcac atcaacagaa tattgtacct gttgtcgatt tggccttaaa aaaagcaggt
     4321 attactaaaa agcaacttca tggtattgcc ttcacacaag gtccgggttt aatgggctcc
     4381 cttttagttg gaagttcttt tgctaaatca ttttcaatgg cattaggaat tccgctcttg
     4441 gccgttaatc acatgcaagc tcacatttta gctcatttta ttgatgagga aggttttgat
     4501 aaaccaactt ttccattttt agctttaacc atttccggtg gtcatactca aatcgtgaaa
     4561 gttaaccact tttttgatat ggaaattatt ggtgaaacaa ccgatgatgc cgtgggtgaa
     4621 gcctttgaca aaacagctaa aatcttaggt ctaccctatc ctggtggtcc tttgattgac
     4681 caatttgcta aagagggaaa tccaaaagca tttggattta caaaacccaa agtagaaggt
     4741 ttaaatttta gtttttcggg tttaaaaaca caaattttat atttcgttca aaaaaacaca
     4801 gcattaaacc caaattttat tgaagaaaac aaagcagata tctgtgcttc tgttcaattc
     4861 actatcattc aaattttaat ggaaaaatta aagttggctg tcaatcaaac cggcattaat
     4921 caaattgcta ttggtggtgg tgtttctgct aattcaggaa ttcgaaaaac gttgaaagag
     4981 gcagaagcaa aatatggttg gaaaacatat attccaaaat ttgaatatac cactgacaat
     5041 gcagccatga ttggtattgt tggttaccaa aaatacttac ataatcaatt tgaaaaggcc
     5101 tctgtcgttt caaaagcaag aattgaattt taatgcaact tttttatttt caacatatag
     5161 ataaagattc taaacaattt tcttttgaca aagaagaaag caaacatatc aataaagttt
     5221 tacgtaaaaa ggaaggcgat atactattcg taaccaatgg tttaggcttc atatttaaaa
     5281 cggaaattaa tttagcatct gattcaaaat gtaccgtaag tataatctct tttgaacaac
     5341 aggtaaaatc aaaaatccat ttgcatttag ttgttgctcc aaccaaaatg aatgatcgat
     5401 atgaatggtt tttagaaaag gcaacagaaa ttggagttca agaaattact cctattattt
     5461 gtgagcattc tgaaagaaaa gttataaaat cggaacgttt tgacaaaatt ttgctatcag
     5521 caatgaaaca gtccaatcaa tattttttac ctattcttaa tgaatctatc acattgaagg
     5581 aatttctaaa aaaagaaatt gtaggtcaaa aattcatagc tcattgtgaa gaaactaaca
     5641 aaaaatcatt gaaagattgt ttacaaaaag gtgaaaattg caccatttta attggaccgg
     5701 aaggtgattt ttcatcaaag gaaattcaat tagccctaga gaatcaatat attcccatat
     5761 ctttgggaaa tactcgtttg cgaacagaaa ctgctgcagt tgtggcttgt cattcggttg
     5821 tatttgaaaa tgaataaatt cgttttatga aaaacatttt tttagccttt ttaattttaa
     5881 ttggatttca aacttttgct caggaaattg ccttactaaa atataatggc ggcggcgatt
     5941 ggtatgctaa tccaacttca ttacccaatt taattaaatt ttgtaaccaa aatattagta
     6001 ccaatctaaa aaataaaccg gcaactgttg aaccctgtag tccagattta ctctcgtatc
     6061 cgtttgttca tatgacgggg catggaaatg ttgtttttaa tgataatgaa atcttaaatc
     6121 ttagaaacta tttaacttcc ggaggattta tacacattga tgataatttt ggaatggaca
     6181 aatacattcg taaagaaata aaaaaaatat ttcccaatag tgatttggta gaaattccgg
     6241 ctacgcatgc catttttcaa aagccatatc ccttcccaaa cggattgcct aaaattcatg
     6301 aacacgaaaa tcaaaaaccg caagcatttg gaattttcat tgaaggcaga ttagtactat
     6361 tttacacctt tgagtgtgat ttaggtgatg gctgggaaga tgaagaagtg cataatgacc
     6421 caaaagaagt aagagaaaaa gcattgaaaa tgggtgcaaa tttagtccat tatgttttta
     6481 caaattagtt caaattaaat cgttaatttt ataactaata catttttaaa atgacatcaa
     6541 aaataatagc cgatggaatc ttaaaagcaa taggcaaatt aacattaata atgctggtat
     6601 tgtttttcat ttatgccatt cgaaccatct tgatttattt gatagtagcc ttaattctaa
     6661 ccctaatttt aaatccaata gtagagtttt ttaaaaacaa atgtaaattc aataatttat
     6721 gggcagtcat taccaccttt acaatattgg tagtggccgt tagtttattt attttaatgt
     6781 ttataccttt aatttcttca caagcaaata gtttatcatt attagatact aatgccattc
     6841 aagaaaaaat aactttatta tattatgatg tgagtttatt tttaatagat agaggtattt
     6901 taatggagga aattttaaaa gatttcgaca taaattctat tttaaattta agtattttgc
     6961 cttccttttt taatggtctg ctttcaacga ttagttcaat agggattgga attgcttctg
     7021 tcttttttat cacctttttc tttttaaaag ataaagtact ttttatcatt ggagcaaaac
     7081 aaattatccc tgacagtcat gaagaagaaa ctttgaattc aattcataaa acttattatt
     7141 tattgtctcg ctattttatt ggattattgc ttcagctttt tatcgttttt attttatact
     7201 acattgtttt acttattttt ggaatacata atgctttaat tatagcttta ctttgtgcta
     7261 tactaaacat cattccatat ataggtccaa caattgcttc ttttttagct gctatacttt
     7321 caatgattgg tcatattggc gaagattttc aaacagttat gttgccctct acgatttata
     7381 tcttaattgc tttttttatt gtgcaactca ttgataataa tgtgaaccaa cctcttattt
     7441 tttcaaaaag tactaaatct catccactcg aaatcttttt agtaatatta attgctggtt
     7501 ttttaacggg aattctagga atgataatag ctgttccgct ttataccatt attaaagtaa
     7561 ttgccaaaga attttatcct aataataaat ttgtgaaaat tctcaccaaa aacttataat
     7621 ttattgccga ttggaaaagt ttaagcaatt attaacggaa gaagttcaac aatttattaa
     7681 tgatcgaatt ggcaaatctg tttctgattt agcttttcaa aaaaatccat ttcctcaagt
     7741 tgattggaaa cttatgctta atcaaattga agcaaaatcg aaagcaaaag acaaacttcc
     7801 aacttggttt tcaaagtcta aaataatatt tccgagcaaa gtagctgttg aacaaacttc
     7861 ctctgaacaa acagctcaat ataaatctag tttaatagtt ggaaactcgc ttatagattt
     7921 aacaggaggt tttggtgtag atactttcta tttttccaaa agattttcta aggttaatca
     7981 ttgtgaacga aacaatgaat tgtcagaaat tgttaaacat aattttagtc aattaaaagt
     8041 taataacatt aattgcattt ctggtgatag tgaattgatt ctttctgatt taaatcaaaa
     8101 atttgattgg atatttatcg atccatccag aagaaatgat gcaaaaggaa aagttttcat
     8161 actcagtgat tgcgaaccta atgtgcctga attattggat ttttattata aattcagctc
     8221 gtcaatttta atcaaaaccg cacctctact cgatttgtca gccggattga aagagcttaa
     8281 aaacgtggcg accattcata tcattgccct tgaaaacgag gtaaaggaac tactatggga
     8341 gattaagcaa aatcattctg attttccaat aataaaaaca gtgaatatca ctaagaaagg
     8401 tatagaaaaa tttgaattta aaaagtttac cgacacacaa aaacctttat tcagtgaacc
     8461 tgaaaaatat ctttatgaac caaatacagc agtaatgaaa tcaggtggtt ttgatgaagt
     8521 ttccagtcaa tttgatataa aaaaattaca tcaacattca catttataca cttcagataa
     8581 agaaataact ttttgtggta gagtttttga aattcaaaaa caactatcat acaataaaat
     8641 agaaatgaaa ctttttttag aaaatcaaaa agctaatgta actgttcgaa attttccgga
     8701 aaccgttgaa aatattagaa aaaaatggaa aattaaagac ggtggagaat tgttttgttt
     8761 ttttactact gatttaaata ataataaaat tgtgttactt tgcaaaaaat taaaatagct
     8821 tatatgaaat tgataatttt cagcttaata cttttgttct ccttacaaat aacagcccaa
     8881 aaaaaatgtg attatactgt agaagttaat gatagtcttg gtactttaaa aacaacaaaa
     8941 gaaattttaa tgtttgaaag acgttttggg aacaatgaaa attatatttt cttttctctt
     9001 tcccttgaca atggaactcc tgttttaaat tttcagcagg ttcaaaaaaa taatgaattt
     9061 atcaaagctt tttgtattga tgcctctaca aaattgtatt tacaattaga aaacggaaaa
     9121 attattacgc ttgttcatac caatactgaa aattgcggaa ctttctttag aaatgaagat
     9181 aaaaatgtac gtgttttatc agctaatttt ttgtttctta aaaatactat agaggaatta
     9241 aaagcaagtc cggtcaactt aatgagggta aaatacagta ctgaaacagt cgattatctg
     9301 attaaacctg aaatgcaatc tgaactttta aatgaattca tcagacccga aaattatttt
     9361 attgattatt tacattgtgt agaataattc aattacaatt ccatttaaag tttgaaatag
     9421 gatccagttc atcactgatt aaatgataat gccaccattc tgattgaaat gctttgaacg
     9481 aacagctttc cataatttct tttaataatt ttctgttttg aaggactttt tcgggtaaat
     9541 cagtataatt atgagaagct tcttttccga aataatcaaa agtagttccc atttcaagtt
     9601 ctaaaccatt tttatcaact aacgtaatat ccaccgcccc gcctctatta tgaatggaac
     9661 ctttactagg atcagcaacg taaattggat tgggaacaat tttccacatt ttcttttgaa
     9721 tggctaacgg gcgataacaa tcataaagtt taattcggaa acccaaaact ataaactttt
     9781 gattcgcttc aatcagtttt ttcacagttt tataacgcaa ataacattct gcacaatcat
     9841 atactttttc ttttaaaaaa tttgatgttg ttgcataccg catatcataa acaatatcgg
     9901 tagaataatc tttaagattt acaaaagttg tatcctgaat agtatgttgc tcattttgct
     9961 ttggaatttt ttcttgactg tatcccaaaa cagaaaataa aagaaaaatt aaaaagaaaa
    10021 gtatcttatt cataatcaaa attttgacta aatatactca aaaagaagtt agtctaaaaa
    10081 ttcagcccaa tattgatgaa tcaattcatg gtgttgctga taaaataact gtgctttttc
    10141 tcgagaacga aaagctaaaa attggggaag tattgaaata ccaatgtaag ctgaaaattg
    10201 tccatcttta atttgtggga aaatagcata atttgctaaa tgatgattcc aaccactatg
    10261 aaatgatttt aacaactgac taagttgagc ttttgctaat gtagcttttg cttgattttc
    10321 agtaacaaaa acatttctgt tttcatcatt tattggacga ttctcgtaag cttcaatctt
    10381 tgaaaaacta tcaatataat agccacttaa ctcgtttaaa tcttgccaac tcgcaacgag
    10441 tttcggattt tcttcgaggt attttcttaa agctcctagt tcatctagat tttttttctg
    10501 ttgaagttct agagcattta aacggttaat tatattcaat ttttcttcag gcgacatagt
    10561 acaaccttaa ttttgcataa aaaattgcga gtagtatttg gggctactga caagtactta
    10621 taaaaagttc cgaaagataa tatattatgt cttaatatta tattattacg ttttaaaagt
    10681 aaaaaaaatc gtttaagagt aaaaaattta ctcagttaaa ttaatttcaa aaaaatattt
    10741 tttaatttaa aaaattaaaa gtaaataata ttttgtttaa ctatttaaaa ttatattaaa
    10801 caaaaaaata tctgtttttg attaaaaaaa gattaaaaaa acaactataa atacaaatat
    10861 taaactgtat ttcaataata tacaataaat acaaatgtga aaaattgcaa attaaaagtt
    10921 aaattattta attctatttt tgtttattat tatttttaca ttgttaaaca aaaaatataa
    10981 aacaatgaaa aaaattacaa acctaatctt tttgtgttca cttctgattg tttcagttgt
    11041 gtcggctcaa aatgctagac ttcaggtaat tcacaattca cctgatttag cagctgcttt
    11101 agttgatgtc tatgtcaatg acgatttact tctaaatgat tttgctttca gaactgcaac
    11161 accttttatc gatgttccgg ctggagtaga attaagtatt gacatcgctc cctcaaccag
    11221 tacatcttct gcagaaagcc tttacaattt aacggctaca ttgacagcag acgaaactta
    11281 cattgttgta gccaatggaa ttgtatctcc ttcaggatat tcgccggcac aaccttttgc
    11341 tttgaatgtc tttgctcaag gtcgtgaagt tgcttcaaac tcagctaaca cggatgtatt
    11401 agtaaaccat ggttctccgg atgctccaac tgtagatgtt gtagaaacat ctgtacctgc
    11461 cggaacaatt gtgaatgata tctcatttcc tgattttgca ggttatttag aacttccaaa
    11521 tttagatttt accctagatg ttcgtgatgc tacaggaact gtaactgttg cttcttatca
    11581 agctccattg caaactttag gattagatgg tgctgccatc acagtattgg ccagtggctt
    11641 cttagatcct gctgtaaaca gtaatggtcc tgcatttgga ttatgggttg ccttagcttc
    11701 aggtggaaat ttaattcctc tacctttagt ggaaactcca acagctcgtt tacaagtaat
    11761 tcacaattca cctgatttag cagctgcttt agtcgatgtc tatgtcaatg acgatttact
    11821 tctaaatgat tttgctttca gaactgcaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnc tgtaaacagt aatggtcctg catttggatt atgggttgcc ttagcttcag
    12001 gtggtgaatt aattccattg ccacaaattc cattaagtgt taccgatttt aatagctcca
    12061 aaatttctat ttatccaaat ccaacatcaa gtcaattgaa tcttgatatt ccatttgact
    12121 atgaaaattt taaaggtata atttatgatt tgagtggaag agaagttaaa actttttcaa
    12181 atccaaatag tatttatgtg agtgatttaa ataatggttt atacatccta aatgtaactt
    12241 tagatgaagt aaattttaat caaaaattct tgaaaaatta acaaatttaa tcagtgtttt
    12301 taagaggtca tcagcaatga tggccttttt ttataaaaaa ataatatcac ttcaatatag
    12361 tttcgcaaat taattatttt ataaaaacaa aaaaccacct gaaaatcaga tggtttttgt
    12421 gacccagtca ggattcgaac ctgagaccta ctgcttagaa ggcagttgct ctatccagct
    12481 gagctactgg gccattcgtc ggggtggcag gattcgaacc tgcggcctcc tgctcccaaa
    12541 gcaggcgcga taaccgggct acgctacacc ccgaaaaaaa gcggagagac agggactcga
    12601 accctggcga cagttacccg tcgacagatt agcaatctgc tccgttacca ctccggcacc
    12661 tctcctaatt ctgcaaagaa cttaactttt ttgcggttgc aaatgtaact tttcattcta
    12721 tactttacaa ctatttaaac tctttttttg atattttttt atttaagttc taaaatcagt
    12781 taaaagtcaa ccacatagga ttaagtgttg tttaatataa aaaaataaag tgcttttaaa
    12841 taaaaaaata gattcaatat ttttttgaat ctaaaaattt taaaatttac atttttgttt
    12901 ataaaatagc tttaaaatgg ttaaattcgc aacgtaaaca aacaaactta aatctggtta
    12961 tgaataatac aaaaatagtt atcgtttcgg cagtgagaac tccaatagga agttttatgg
    13021 gtggactatc tactgttccg gcacctcaat taggagcaat tgccattcaa ggtgctttag
    13081 ctaaaataaa attagaccca aaattgattg atgaagtgtt tatgggtaac gtggtgcaag
    13141 ccggagttgg tcaggcaccg gccagacaag ctgcaatttt agcaggatta ccaaatactg
    13201 ttgcctgtac tacaataaat aaagtttgtg cttccggaat gaaagctctt atgcttggag
    13261 cacaagcaat aaagagtggt gatgcagaaa tagttgtcgt tggaggaatg gaaaatatga
    13321 gtttgatacc acattatgtt catttaagaa acggctataa attcggcccg gcatcaatga
    13381 ttgatggcat gcagaaagat ggcttagtag atgcatatga taacaatgcc atgggcgttt
    13441 gtgcagattt atgtgcctca gaatataaaa tatcaagaga agctcaagat aattttgcaa
    13501 ttcaatccta tgaacgttct gccaaagctt ggtcagaagg aaaatttgac aatgaaattg
    13561 ttccggttgc tgttccacaa agaagagggg aaccaattat tatttcaaaa gatgaagagt
    13621 ttaccaatgt aaaactggat aaaattccta gtttaaatgc tgtttttaca aaagacggaa
    13681 ctgtaactgc tgccaatgct tctacaataa atgatggtgc tgctgcaatg attattatga
    13741 gtgaggaaaa agcaaattct ctaggattaa aacccttagc ttatgttaga ggttatgccg
    13801 atgctgcaca agagccaaaa tggtttacaa ctgctccggc aaaagcatta ccaaaagcat
    13861 tagacaaagc cggtatttca atttccgagg ttgatttttt tgaatttaat gaagcttttg
    13921 cggttgttgg tttagccaac tcacaattac ttggattaaa cgaatcaaaa attaatgtca
    13981 atggaggtgc tgtttcctta gggcatccgt taggttgttc aggagcaaga ataattgtta
    14041 ctttactaaa tgttttacaa caaaacaatt caaaaatagg tgctgcggct atttgtaacg
    14101 gtggcggtgg tgcttcagca gttgtaattg aaagagcatc ttaacacttg aaatagcccc
    14161 aaattcatgt acggaatctg taatcttgct atcatacctc tacgaattga acctaacgat
    14221 cgaagcgaac aagtgtcgca agttttgttt ggtgagcatt ttgagatact agagcaactt
    14281 aagcaatggt ttaagatacg tttagagttt gatggatatc aaggatggat tgattcaaaa
    14341 caatgccaaa ttatatctga aatggatttt aatgaaattt caggcagttt acaaatactc
    14401 aacaatgatt tagtcgaata tattaccagt gattcaaatt tattgatgcc agtacctcta
    14461 ggggcttcac taactttttt aaattacgaa ggaataaaca aatacaatta cgaatttgaa
    14521 ggaatgaagg caacgggtgt aaaacctaaa tccgaattat taaaaaccgc tttcatgtat
    14581 ttgaatgctc cctatttatg gggaggtaag acaccttttg gaatcgactg ttccggtttt
    14641 acacaaatgg tatataaact taacggctat aaattattaa gagacgcttc acaacaagcc
    14701 acacagggag aagctttaag ttttattgaa gaaagtgaac ctggtgacct agcctttttt
    14761 gataatgaag aagggaaaat aatacatgtt ggcatcatta tgcatgacaa ttatattatt
    14821 catgcgagtg gtaaagttcg tattgaccgt ttagatcatt taggaattta taatgctgat
    14881 actaatcgac atactcataa attgagagtt attaagaaaa ttatctaaaa aaaacacccc
    14941 caaatagtgt aacctttttg ggggtattta ttattagatt tgttttgcta ttgtgcttta
    15001 atcgattcta ttttagcttt tgtttcttta tatttatcat ccattcctaa agtgggataa
    15061 atttgggtca tcaaatttaa aatatccaag ttattaggtt tataagttaa tgctgtttca
    15121 agataaggta tagcctcttc aagcaattta ttctttttat ccatcaactc gttgtatttt
    15181 ttattttcag cagatgaatt acctaaacta ttcatttcct caaatattct ggactgctca
    15241 tcaattttaa aatttgctaa attgatatat gcattaacat aatccggttt catttcaatt
    15301 gtttttttga aatattttgt tgcttcaact tcattgttca tttttgtata agcaacacct
    15361 aaattatata ctaaatccgc atcttttgga tttttatcag aagcttcttt tataattcta
    15421 acataatttt caacatcgcc ttttctgtta taaaaatctg cttcaatcaa aacaaagtcc
    15481 atatcattag gatatagttt tctggctgct tgtatttcaa gcaatgcttt gtcaacttga
    15541 cctttttcag ccaaaagaat agagtaattt ctgtatactt cacctcttac agacggaact
    15601 ttttcttctc ttggtttttc atgagtgccg gcttttatat acaaatctct ttcagctttg
    15661 gttccaaagg aagtttcagc ttttgtagct ttatcagttg caaaatattc tgtggccttt
    15721 ccggtataat ttaaatcttt caattgtttt aaatactcaa ttgccgtatc ataatctttt
    15781 gttctgatgg ctccgtttgc tgcataatat aaataaaccg tgtcttttgg actaattcta
    15841 taagcttggt atagtttctc tgtaccttct ttgtatttgt cagctttgtt atcagcaatg
    15901 gcagaattta ccaaattatc gcgaacagtg gctaaagatg cttctgcttg agaagtatac
    15961 ttagattttc ccattgattt ttctaatgca ataagatctt gatacgattt tgctgctaaa
    16021 atcaaattat ttcccgattc aacattgtgg tttgaaagtt ctaataaagc atttcctttc
    16081 agaaaataaa aatgagcttt ttctgcatcc ggtgctgttg ctatgacagc ttctgcagcg
    16141 gctaatgtat ttttaactgc tgttgtttca taatttttgg ttgctttttc caacgctttt
    16201 aattgatctt tttgagccat tgtactcaaa cttaacatca aagcaaaggc tggaatgatg
    16261 tatctacttt tcatggtaaa tcaaatttaa ttaatattta ttcttgggtt tcttcgtttt
    16321 cttctgttgt ttcatcactg tcatcatcaa aatcagttgt ttctattttt gaatcactgt
    16381 ttaaaacggc agctgttaca tcttcaaatt ctacttcttc cacagcttct tctttcataa
    16441 ctttggttac tgcagcaata gaatcatttc ctttaatgtt gattaatcga acaccttgtg
    16501 tggctcttcc catgactctt aaatcagaaa cttccattct gatagtcaat ccagacttgt
    16561 tgataatcat taaatcatca gcatccgtta cactgttaat agaaactaat cgtcctgttt
    16621 tatcagtaat attaagcgtt ttcactcctt taccgccacg gttcgtaatt ctataatctt
    16681 ctaatgaaga acgttttccg taaccatttt cagaaacaac taatatttca ctgctcatat
    16741 catttacaga aaccatgcca attacttcat ctttatcgtc tgctaaagta attccacgaa
    16801 cacctgatgc atttcttccc atcggacgag ttttgctttc ttcaaatcga actaactttc
    16861 cggattttac agccactaaa atttgacttt ttccgttagt taattttgct tctagtaatt
    16921 catcatcttc tttaatagta atagccgcaa ttccattttg tcttggtcta gaatattgct
    16981 ccaacaacgt ctttttaact tgaccttgtt tagtcgccat aatcacataa tgactattta
    17041 tatattcttc atttttcaag tcttgcgtac agatgaaagc ttttacttta tcatcatttt
    17101 caatgtttat taaattttga attgctcttc cttttgatgt tttacttcct tccggaattt
    17161 cataaactct catccagaaa cattttcctt tttgagtaaa gaataacata tattggtggt
    17221 ttgtggctac aaataaatgc tctaagaaat cttgatctct tgtaccggca cttttttgac
    17281 caactcctcc tctattttgt gttttatatt cagataagtt tg
//
LOCUS       DAOTCW010000044        16848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1919_length_16848_cov_42.810159, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000044 DAOTCW010000000
VERSION     DAOTCW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 16848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16848
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1919_length_16848_cov_42.810159"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="P5335_05325"
     CDS             <1..641
                     /locus_tag="P5335_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRZ74333.1"
                     /translation="GVSIGFYLSNVIEKPSFATIFIFIAACLYFYATTLKKVLLLGNL
                     MVAFVLALSVLIIGFFDLYPATHEGNQDQMRMLFGILTDYAFFAFLINFIREIVKDAE
                     DVKGDYNQGMQTLPILLGVGRTSKVIFGLLLVPIGIVFWYVNSYLMFNDLYIAVLFLL
                     FLVLSPLLYCLVKSWSAKTTTDFRHLSLLLKWILFFGILSIVIINYNIKQNA"
     gene            634..1221
                     /locus_tag="P5335_05330"
     CDS             634..1221
                     /locus_tag="P5335_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HRZ74334.1"
                     /translation="MLNEKLKEFHLILASGSPRRQQFFKDLDLDFEIRLKEIEEVYPN
                     HLKAEEITNFLAQLKANAFEGELKANDILITSDTIVWHQNQCLGKPKDYENAFQMLQL
                     LSNATHEVITSVCFKTVDKTETFFEVTKVTFTTLSEEEIRYYLDHYKPFDKAGSYAIQ
                     EWIGYVGISKIEGSYTNVVGMPMHGVYEKLNSFLA"
     gene            1227..1757
                     /locus_tag="P5335_05335"
     CDS             1227..1757
                     /locus_tag="P5335_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRZ74335.1"
                     /translation="MEFLKLYIKEEIATGVVLVISMILRFLIAKIVRRFARMSETLEH
                     RTNLVIKYFNLLIGTIATIALFVIWGVKTQDVILVVSSLFAVIGVAMFAQWSILSNVT
                     SGIIIFFSAPFKIGDTIKIHDKDFPIEGEIDDIRGFHTFLKTKDGELITYPNSLLLQK
                     GISVIKNTVVEKEFFD"
     gene            1810..3639
                     /locus_tag="P5335_05340"
     CDS             1810..3639
                     /locus_tag="P5335_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74336.1"
                     /translation="MPTRIILLITVFFCFLNSYSQEKKTATDSTLLGYQKIENTAKKS
                     KFTASLHRLIFKSTSVKQTGIKTTQIVSNYSQFEGKIIRKISIHTLDPFGFSDSDSTR
                     TPRHFASKAGNAIHIKTKKFAIRNVLLIKENEPFDSLKVKETERLIRSQRFVRRVTVY
                     TENVSGSSADSVDVKITVLDSWSLIPTTSATTNKASYELLERNFLGSGHSWDNKYTHE
                     LNDKRRAFSTRYIIPNIKNTYIQTTLNYQMDVNKDYSKYIEIERKFFSPLTRWAGGVL
                     LENRLLKDSLIDSNSNYELQTFKYGTIDAWGGHSAPILKGNSETERTTNLITTLRYYK
                     RNYERRISEDFDPYAFYTNEAFWLTGIGVSSRKYVKDEYIFNYNITEDVPIGKYYGIT
                     AGIQDKNDNTRFYVGARATLGDYFKWGYFSTNYEYGTFFNDGKNEQSAFLAELYYFTR
                     LFEPGKWKIRQFFKSNMVFGGHRLDIKGDQLSINEANGITGFNDAKLIGTKKMIFSFQ
                     TQSYSPWELAGFRFNPFFNYTLAFIGDSENGFKKSEGFSKIGLGILLSNDYLVFSTFQ
                     FSIAFYPKIPNEGRNIFRTNNFDTRDFGYLDFEINKPRTVLYE"
     gene            3816..4229
                     /locus_tag="P5335_05345"
     CDS             3816..4229
                     /locus_tag="P5335_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74337.1"
                     /translation="MKTVNYLTLFLFLIIGTIATSQSYIIESQKDLIKQSKVSLDSTR
                     YYYFPNLEAYYDTKISMYIFKRKGEWVTESHIPANYRGYSLFNKRYEIINGLVDNDTP
                     QEFIEQHRKEYPPIFKAKDMKIKLDKEKNSSLTYN"
     gene            4407..4898
                     /locus_tag="P5335_05350"
     CDS             4407..4898
                     /locus_tag="P5335_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74338.1"
                     /translation="MKTLKSIILGMFMVVVATAFGQVSLNVNVGTPPSWGPAGYSSAR
                     YYYLPEIEVYYDVQQANYIYLGNGKWYRSNHLPNRCKHYDLHRGHKVVLTNYHGSSPY
                     SFYHNHKVKYPKNYKGNGHNSTAHNDNHQYKSKQYKGKSHSNGNDNSNNKGGKNHSKG
                     KKN"
     gene            5022..7529
                     /locus_tag="P5335_05355"
     CDS             5022..7529
                     /locus_tag="P5335_05355"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRZ74339.1"
                     /translation="MVKNCYTFLLFFFLWLSAIHAQQPSKPSAVEIYHQLEKLNFLGS
                     VLYIAAHPDDENTRLISYMSNHLHARTGYLSLTRGDGGQNLIGPELRELLGVIRTQEL
                     IEARKIDGGEQFFTRANDFGFSKIPDETLRIWNKNQVLEDMIYIIRKFKPDVIINRFD
                     HRSPGTTHGHHTSSAVLSIELFNQVNDATVFPEQLKYTSLWQPKRLYFNTSWWFYGGR
                     DKFEKADKSKLTNFDMGVFYPESGKSNQEIAALSRSRHQSQGFGSTGTRGEEVEYLEF
                     ILGENPTPKNAIFEGIDTSWNRVQGGTAIGVILAQVKKEFDFKNPAASIPKLVESYQL
                     VQNLKDEHWKKIKSNELKKIIAGCAGLYLEAVAETQSATPGSVLKVKLESINRSAQKM
                     NLNGITTTPTSTIVFNQSTLEPNKAVFSDIELIIPSTQEYTSPYWLKEQGTVGMYAVS
                     NQQQIGIPDVIRTISVTFSVEINGVVIPFEKEVIYKFNDEVKGEVYKPFDIVPVVTTS
                     ILDKVKIFNSEKSKTIGVKIKAGKENVSGTAHLEVNSGWKVTPNAIPFQLNRKDAETT
                     VYFEVTPPKNSSEITISSYAMVDGKKENYEQTIIQYEHIAQQQILSTSEAKFRKLDLK
                     TNSEKIAYIMGAGDEVPQYLSQMGYSVTILKPDEITSENLKNFQVVVLGIRAYNTVDE
                     LAFKQQLLFDFVKEGHTMIVQYNTNGKLTVKDLAPYPLKLSRDRVTEEDAEMRFLAPN
                     HPVLNYPNKITSKDFEGWVQEQGLYYPDEWDATFTPLLSSNDKGEEAKNGGLLVASHG
                     KGFYIYTGLSFFRELPAGVSGAYRLFANMISIGTKSN"
     gene            7645..>8108
                     /locus_tag="P5335_05360"
     CDS             7645..>8108
                     /locus_tag="P5335_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRZ74340.1"
                     /translation="MQTLDWFILIFSLLFIVVYGAWKTKGSQDVKDYILGNNESKWWT
                     IGISVMATQASAITFLSTPGQAFHDGMGFVQFYFGLPLAMVFICIFFIPIYHKLNVYT
                     AYEFLENRFDQKTRSLAAILFLVQRGLAAGITIYAPAIILSSILGWNLNWLN"
     assembly_gap    8109..8208
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8209..9503
                     /locus_tag="P5335_05365"
     CDS             <8209..9503
                     /locus_tag="P5335_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRZ74341.1"
                     /translation="PAIILSSILGWNLNWLNFIIGVLVIIYTVSGGTKAVNVTQKQQM
                     FIIMFGMFIAFYLILSYLPKEISFSNALQIAGVNDKMNILDFSINPNSRYTFWSGITG
                     GLFLALSYFGTDQSQVQRYLSGKSVRESQLGLIFNGFLKVPMQFFILLTGVMVFVFFQ
                     FNKVPLNFNPTSQIAVEKSIYAADYKNLEERLALIQEEKKIVTLTYAKQINIDVDNPV
                     LRKRIIALSEKEKNLREEAQGIIKKIKQEPNDKDYVFIHFILHYLPTGLVGLLLAVIL
                     SAAMSSTASELNALASTTTIDIYKRNLKSEKSDKHFVHVSKVFTLLWGAIAILFACFG
                     TLVENLIQLVNIIGSVFYGTILGIFLVAFFVKFIKGNAIFYGGVLSQLIIFIIYYFTI
                     HIYPSGKELLGYLWLNAIGAALTLGLSALIQVSMKNKN"
     gene            complement(9574..10425)
                     /locus_tag="P5335_05370"
     CDS             complement(9574..10425)
                     /locus_tag="P5335_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRZ74342.1"
                     /translation="MKKIVLAFTFFVATMTVGAQVKTPAPSPRANFKQSVGLTEVEID
                     YSRPSAKGRAVFGNLVPFGKMWRTGANQNSMITFGDDVVIDGKTLKQGKYAIFTTPKA
                     DNWEVVFYADTENWGTPENWDEAKVALRTNVKPETLTRNVETFTISLNNIANDFAHLE
                     LSWEKTLVALKFEVPTSKLAMASIDKVLAGPTAGDYFSSAQYFFQSNGDMTKALEYVN
                     KAVAMTAEKGTPFWYNRLKSQIQAKLGDKKGAIETAKVSLEAAEKAGNADYVKMNKDS
                     ISEWSKK"
     gene            10568..11035
                     /locus_tag="P5335_05375"
     CDS             10568..11035
                     /locus_tag="P5335_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRZ74343.1"
                     /translation="MKIYTLHTKQNLPISLEEAWEFLSSPKNLKTITPDYMGFNILSG
                     AEKPMFPGQIIQYIVTPILGIPTKWVTEITHVQDKKYFVDEQRFGPYSLWHHKHFLKE
                     IPGGVEMEDIVDYKIPMGILGQLVHPILVKPKLNEIFNYRQKKLIELFGEYKG"
     gene            11037..11729
                     /locus_tag="P5335_05380"
     CDS             11037..11729
                     /locus_tag="P5335_05380"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRZ74344.1"
                     /translation="MKNILLIGGSYGIGLAIAKELQYECKIFIAARTNELSSDLNITY
                     IPFDATTDEFDTTKIPAVLDGLVYCPGSINLRPFKGIKPETFESDLNLNFISLVKVIQ
                     TVLPNLIASEQSSIVLFSSVAASMGMPFHTSVAASKGAIEGFAKALAAEYAPKIRVNV
                     IAPSLTDTPLADKFLNNDVKKEKSAERHPLKRFGKPEDLAQMAQFLLSDKSSWITGQI
                     FHVDGGMSTLLV"
     gene            11752..13038
                     /locus_tag="P5335_05385"
     CDS             11752..13038
                     /locus_tag="P5335_05385"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRZ74345.1"
                     /translation="MNIFWFRRDLRLDDNVGLFHALNSTEEVLPVFIFDESILAELPK
                     DDARVTFIHELLSKIQEQLALQGKSLAVFHGKPIAVFKQLISEHSISTVYTNNDYEPY
                     ARKRDKEINQLLKEHGIDFKTSKDQVIFEKSEVVKDDGLPYVVYTPFSRKWKEKFKTI
                     SLPNYPSENLLDKIALHSYPFLSLTDIGFETSSIKITPFDVSDKLVDQYESTRNFPAM
                     SGTSMIGAYLRFGAVSIRKMVAKAIASKNETFWNELIWREFFMQILWHFPHTIHKSFK
                     EKYDAIQWNNNETEFQKWCEGKTGYPMVDAGMRELNATGHMHNRVRMVVASFLCKHLL
                     IDWRWGETYFALKLFDYEQASNVGNWQWAAGSGVDAAPYFRIFNPAEQIKKFDKDLKY
                     IKKWVPEVETSAYSLPIVDHKEARERCLRVYKEAVG"
     gene            13111..13368
                     /locus_tag="P5335_05390"
     CDS             13111..13368
                     /locus_tag="P5335_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74346.1"
                     /translation="MKNRLIMLTILATATLFSCAKKEEKPAEVIVIEQPAEQAAPVEQ
                     PAPVENTDGTTIEVDENGLKVNSEKTSVDIKDGKAKVEVKN"
     gene            complement(13404..14060)
                     /locus_tag="P5335_05395"
     CDS             complement(13404..14060)
                     /locus_tag="P5335_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRZ74347.1"
                     /translation="MIQTVIFDMDGVIVDTEPVHHFAYQQHFKQLNIDVSPEMYASFT
                     GNSTKNIYERLKSVYNLQQDVKTLVEVKRNLFNDAFDQKKDIYLLDGVEELIKELHQN
                     EMQLVLASSSANVTIERIFNRFDLHKYFTHKVSGEDFPNSKPHPAIFRQAAFLAKTPT
                     ENCIVIEDSTNGILAAKAAGIYCIGYDSVNSKLQDYTLADKVISHFNELSFEKIQLIN
                     "
     gene            complement(14285..15001)
                     /locus_tag="P5335_05400"
     CDS             complement(14285..15001)
                     /locus_tag="P5335_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006261982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRZ74348.1"
                     /translation="MTKWQERAQLLFKTEGLNKLKNANVLVVGLGGVGSFAAEFLVRA
                     GVGNITIVDGDVVDITNINRQLPALHSTIGMPKVTIVGNRLMDINPELNLVRVEEFLS
                     PERAMEIVSEEFDYVLDCIDSITPKLNLIIAAKRKRVKIISSMGAGGKMEASKVKVSD
                     ISKSENCFLAKTMKRRLKEVKIDKLKVVFSSEIQDESSLKLTDGNNFKKSFYGTNSWM
                     PGLFGLYAAEAVVKYLLEKS"
     gene            complement(15114..15779)
                     /locus_tag="P5335_05405"
     CDS             complement(15114..15779)
                     /locus_tag="P5335_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRZ74349.1"
                     /translation="MNYFNLHTHQFTNQQTRLELVNQYPWEFDESIPTYSIGIHPWYI
                     DEQRLQQDLKKIDEKLALPECLALGECGLDKRIDIPLQLQIEVFEKQISLAEKHQKPL
                     VLHCVSAFQELIEIKKRVKITVPMIVHGFSKNEQTAKQLINSGFYLSFGKYLIRNPEL
                     KTVFQSIPNDKFFLETDTIEESLEEVYALAAKYKNCEIEEMKAIIASNFEKVFSRSLV
                     IPK"
     gene            complement(15779..16369)
                     /locus_tag="P5335_05410"
     CDS             complement(15779..16369)
                     /locus_tag="P5335_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HRZ74350.1"
                     /translation="MKILFLLLIAQLSYAQKFEKDSVLQFQKELNEHYADTASSPLKK
                     EDLKHFVNLEFYPISETYFVQAKFTRTKKEKPFEMKTTTARKPIYVKYGELQFTINDV
                     PCKLNVYQNVEYAKKPGHQDSLFLPFTDLTSGVESYGGGRYIDLEVQKGNTWTIDFNK
                     GYNPYCAYNEKYSCPIPPAENDLKVEVKAGVKKYHD"
     gene            16386..16520
                     /locus_tag="P5335_05415"
     CDS             16386..16520
                     /locus_tag="P5335_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74351.1"
                     /translation="MNYELRISVWLLVVSNLINWNGIMGDFKGFNRFFVEGLNTDEVD
                     "
BASE COUNT         5243 a         2530 c         3165 g         5810 t
ORIGIN      
        1 ttggtgtcag tattggtttt tatttgtcga atgtgattga aaaaccaagt tttgcgacta
       61 tttttatttt tatagcggct tgtttatact tttatgccac gacgctgaaa aaagttttgc
      121 tattaggtaa tttaatggtt gcctttgtat tagccttgag tgttttaatt attggttttt
      181 ttgatttata tcctgctacg catgaaggca atcaggatca gatgcgcatg ttgtttggca
      241 ttttaaccga ttatgccttt tttgcctttt taattaattt cattcgtgaa atagtgaaag
      301 atgcggaaga tgttaaagga gattataacc aaggtatgca aaccttacct atccttttag
      361 gagttggacg aacttcaaag gttatttttg ggttgctttt agttccaatt gggattgttt
      421 tttggtatgt gaacagttat ttgatgttta atgatttgta tattgctgtt ttgtttttgc
      481 tgtttttagt tttatctccc ctcttgtatt gtttagtaaa aagttggagt gcaaaaacta
      541 cgactgattt tcgtcatttg agtttacttt taaaatggat attattcttt ggaattttgt
      601 caattgttat cattaattac aatatcaaac aaaatgctta acgaaaaatt aaaagaattt
      661 catttgattt tagcttcggg ttcgcccagg aggcaacagt tttttaaaga tttggattta
      721 gactttgaaa ttcgtttaaa agaaattgag gaagtctatc caaaccattt gaaagcggaa
      781 gaaatcacca attttttagc ccaattaaaa gccaatgcct ttgaaggcga actcaaagca
      841 aacgatattt taattacgag tgatactatt gtttggcatc aaaaccaatg tttgggaaaa
      901 ccgaaagatt atgaaaatgc gtttcaaatg ttacaattgt tatcgaatgc tacgcatgaa
      961 gtgattacat cggtttgttt taaaacggtt gataaaaccg aaaccttttt tgaagtgaca
     1021 aaagttactt tcactacact ttcagaagaa gaaattcgtt attatttaga tcattataaa
     1081 ccctttgata aagctggtag ttatgccatt caggaatgga ttggctatgt tggaatcagt
     1141 aagattgaag gttcatacac caatgttgtt ggcatgccta tgcatggtgt ttatgagaaa
     1201 ttaaattcat ttttagctta gttaatatgg aatttttaaa actttatatt aaagaggaaa
     1261 ttgcaacggg agttgttctt gtaatttcaa tgattttacg ctttttaatt gctaaaatag
     1321 ttagacgttt tgcccgaatg agtgaaacgt tagaacaccg aaccaattta gttatcaagt
     1381 attttaattt attgatagga actattgcca cgattgcttt gtttgtgatt tggggagtta
     1441 aaacacaaga tgttattttg gtcgtttctt ccttgtttgc tgtgattggg gtggccatgt
     1501 ttgcacaatg gtcaattttg agtaatgtta cctccgggat tattatcttt ttttcagctc
     1561 cttttaaaat tggtgatacc atcaaaatac atgataaaga ttttcctatt gaaggcgaga
     1621 ttgatgatat tcgcggtttt catacctttt taaaaacaaa agatggcgaa ttgattacct
     1681 atccaaatag tttactttta cagaaaggaa tatccgtgat taaaaatact gtggtagaaa
     1741 aagagttttt cgattaaaaa agcgtatctt atcttgatgc taattttgta aaactagcgt
     1801 tatcgtttta tgccaacaag aattattctt ttgattactg tgtttttttg ttttttaaat
     1861 tcttattctc aagaaaaaaa aacagcaacc gactcaaccc tactaggtta tcagaaaatt
     1921 gaaaatacag caaagaaaag taaatttaca gcaagtttgc atcgtttaat tttcaaatcg
     1981 acatcagtca aacaaaccgg aataaaaacg actcaaattg tttccaacta ttcgcaattt
     2041 gaaggtaaaa ttattcgaaa aatatcgatt catacacttg atccatttgg gttttctgat
     2101 tctgactcta caaggacacc ccgccatttt gcttcgaaag caggtaatgc tattcacatc
     2161 aaaactaaaa aatttgctat tcgcaatgtc ttactgatta aagagaatga accatttgac
     2221 agtttgaaag taaaggaaac cgaacgatta attagaagtc agcgtttcgt tagaagggta
     2281 accgtttata ctgaaaatgt atcagggtca tctgctgaca gtgttgatgt taaaattact
     2341 gttttagatt cgtggagttt gatccctact accagtgcta caacaaacaa agccagttat
     2401 gaacttttgg aacgtaattt tcttggaagc ggacattcat gggacaacaa atacacacat
     2461 gaattaaatg ataaacgaag agctttttct acccgctaca ttataccaaa tataaagaac
     2521 acttacattc aaacgacact aaattatcaa atggatgtta ataaagatta tagtaaatat
     2581 atagaaatag agcgaaaatt cttttctccg ttaactagat gggccggtgg agttctattg
     2641 gaaaaccgtt tattgaagga cagtttgatt gatagcaaca gtaattatga actacaaaca
     2701 ttcaagtatg gaacaattga tgcctggggc ggacattctg caccgatatt aaagggaaac
     2761 agtgagacgg aaagaactac caatttgatt acgaccttgc gttactataa aagaaattat
     2821 gagcgaagaa tttcggaaga ttttgatccg tatgcatttt atacaaatga ggcgttttgg
     2881 cttacgggaa ttggcgtttc atctcgaaaa tatgttaagg atgaatacat ttttaattat
     2941 aatattacgg aagatgttcc gattggaaaa tattacggaa tcactgcggg tattcaagac
     3001 aaaaatgata atactcgctt ttacgttggt gcccgagcga ccttaggtga ttattttaaa
     3061 tggggttatt ttagtacgaa ctatgaatat ggtacttttt ttaacgatgg taaaaatgaa
     3121 caaagtgctt ttttagcgga attgtattat ttcacgcgac tttttgaacc ggggaaatgg
     3181 aaaatcaggc agttttttaa aagcaatatg gtttttggag ggcatcgatt agacattaaa
     3241 ggagatcaat tgtcgattaa tgaagcgaat ggtattacag gatttaatga tgctaaactg
     3301 attgggacta aaaagatgat tttttctttt caaacacaat cttactcccc atgggaacta
     3361 gccggatttc gatttaatcc gttttttaat tatacgttag cgtttattgg tgattctgaa
     3421 aatggtttta agaagagtga gggtttttct aaaattggtc ttgggatttt gctttcgaat
     3481 gattatttag tttttagtac ttttcaattt tcgattgctt tttatcctaa aataccaaat
     3541 gagggaagga atatattcag aacaaataat tttgatacgc gtgactttgg ttatttagat
     3601 tttgaaatca ataaaccccg tactgttttg tatgaataac gatactttaa actaaaattc
     3661 atccccattg ttaggtattt tgtcaaattg ttggctcaat attaacagca caattggtta
     3721 ttattttgta tatttactac aagttttacc catactgata actgcaaaga ataccacagt
     3781 tcgtttttga ttaatttttt aaaaattaga atgaaatgaa aactgtcaat tacttaaccc
     3841 tttttttatt ccttattata ggaactattg caacgagtca atcgtatatt atagagtctc
     3901 aaaaagattt aataaaacaa tcgaaagttt ctttagattc aacacggtat tattattttc
     3961 cgaatttaga ggcttattat gatacgaaaa tttcgatgta tatttttaaa cgtaaaggcg
     4021 aatgggttac tgaatctcac attccggcaa attatagagg ctatagttta tttaataaac
     4081 gctatgaaat tataaacggg ttagtggata acgacactcc gcaagaattt attgaacaac
     4141 acagaaaaga atatccgcct atttttaagg caaaagacat gaaaattaaa ctggacaaag
     4201 agaaaaatag tagtttgacc tataattaga attggttttt aaattaagta aggcagtctg
     4261 aatggagcta atctgtttag actgttttat tttggttgaa ctcaaacgac taaagtttac
     4321 ataattccta ttttattggt gaactaaatt tattggcata ctagttgact attataaagt
     4381 aatggcactt aaaaaaaaat gaaaagatga aaacacttaa atctattata ctgggaatgt
     4441 ttatggttgt tgtagccact gcttttggac aagtttcttt gaatgttaat gtgggcactc
     4501 ccccttcctg gggcccagcg ggatattcgt ctgcacgtta ttattatttg ccggaaattg
     4561 aagtttatta tgacgtgcaa caagcaaatt atatttattt aggtaacggg aaatggtata
     4621 gaagtaatca tttgcctaat agatgtaaac attatgattt gcaccgtggt cataaagtag
     4681 tgcttactaa ctatcatggc tcttcccctt attcgtttta tcataatcat aaggtaaaat
     4741 atccgaaaaa ctataaggga aatgggcata actcaacggc tcataacgat aatcatcagt
     4801 acaagtctaa acaatataaa gggaaaagcc attcgaacgg gaatgataat tcaaacaata
     4861 aaggaggaaa aaatcattct aaaggaaaaa aaaattaact ataaatgaac taaaacagag
     4921 ccttacatgc tctgtttttt tattactgtt aaagaagttc taaattagcc ctcgaactta
     4981 ggtaatttag tatatttggt ttttacagaa agcaaaaatc aatggttaaa aattgctata
     5041 ccttcctcct ttttttcttc ttatggctct cggctataca tgctcaacaa ccttcaaaac
     5101 cttcggccgt tgaaatttat catcaacttg aaaaactgaa ttttttaggt tccgtgcttt
     5161 atattgccgc acatcctgat gatgaaaaca cccgattgat ttcgtatatg tctaatcacc
     5221 ttcatgcccg aacgggttat ttgtcgttga ccagaggtga tggaggtcaa aatttgattg
     5281 gtcctgaatt acgtgaatta ttaggtgtta ttcgtacgca agaacttatt gaagcccgaa
     5341 aaatcgatgg cggagaacaa ttttttactc gagcaaatga ttttgggttt tctaaaattc
     5401 cggatgagac acttcgcatt tggaataaaa accaagtttt ggaagacatg atatatatta
     5461 tccggaaatt taaaccggat gttattatca atcgttttga tcatcgttct cccggaacta
     5521 cgcatggtca tcacacgtcg tctgcggtat tgtcaataga actgtttaat caggtgaatg
     5581 atgctactgt gtttccggaa caattgaaat atacttcgct ttggcaacca aaacggctat
     5641 attttaatac ttcatggtgg ttttatggag gtcgagataa atttgaaaag gccgacaaaa
     5701 gcaaattaac taattttgat atgggtgttt tttacccgga gtcaggaaaa tcaaatcaag
     5761 aaattgctgc tttgagtaga agcaggcatc aatcgcaggg ttttggcagt acaggaaccc
     5821 gcggagaaga agttgaatat ttagaattta tactgggcga aaatccaacg cctaaaaatg
     5881 ctatttttga agggattgac acttcgtgga atcgtgttca aggcggaact gccattgggg
     5941 tgattttggc tcaagttaaa aaggagtttg attttaagaa ccctgcagcg agtattccaa
     6001 aattagttga aagctatcaa ctggttcaga acttgaaaga cgaacattgg aaaaaaatta
     6061 aatcgaatga actgaagaaa attattgccg gatgtgccgg attatattta gaagccgttg
     6121 ccgaaacaca aagtgcaacg ccgggaagtg ttttaaaagt taaattggaa agcataaaca
     6181 gaagtgctca aaaaatgaat ttgaatggta ttactactac tccaacgtca acaatagttt
     6241 ttaaccaatc aacattagag cctaacaaag ctgttttttc tgacattgaa ttaatcatac
     6301 cttcaacgca ggaatatact tctccttatt ggttgaaaga acaaggaacc gttgggatgt
     6361 atgctgtttc aaatcaacaa caaattggga ttcctgatgt tatccgaact atttcagtga
     6421 ctttttcagt tgaaattaat ggcgttgtta ttccgtttga aaaagaagtt atttataaat
     6481 ttaacgatga agtgaaaggg gaagtttata aaccgtttga tattgttccg gttgtgacta
     6541 cctcaattct agataaagta aaaatattta attctgaaaa gagtaaaacc attggtgtaa
     6601 aaataaaagc cggaaaagaa aatgtttcgg gaaccgctca tttagaggta aattcaggtt
     6661 ggaaagttac tcctaacgcc attccatttc aacttaatag aaaagatgct gaaacaacgg
     6721 tttattttga agtaactccg cctaaaaaca gtagtgagat tactatttcc agttatgcaa
     6781 tggttgacgg gaaaaaagaa aattatgagc aaaccatcat tcaatatgaa cacattgctc
     6841 aacaacaaat actttcaact tcagaagcta aattcagaaa actggatttg aaaacaaatt
     6901 cagaaaaaat agcttatatc atgggagcgg gagatgaagt tccgcaatac ctttcgcaaa
     6961 tgggttattc ggtaactatt ttgaaaccgg atgaaattac ttcagaaaat cttaaaaatt
     7021 ttcaagttgt ggttttgggc atcagggctt ataatacggt agatgaatta gctttcaaac
     7081 agcaacttct ttttgatttt gttaaagaag gccatacaat gattgttcaa tataatacta
     7141 atggtaaact taccgttaaa gacttagccc cttatccgtt gaaattatca agagacaggg
     7201 ttacggaaga agatgcagaa atgcgatttt tagcaccaaa tcatccggtt ttgaattatc
     7261 cgaataaaat aacttcgaag gattttgaag gatgggttca agaacaaggg ttatattatc
     7321 cggatgaatg ggacgcaacc tttacccctc ttttgtcatc taacgacaag ggagaagaag
     7381 caaaaaatgg cggattatta gttgcttcac atggaaaagg cttttatatt tacaccggtt
     7441 taagtttctt tagggaatta ccggccggcg tttccggagc gtatcggttg tttgcaaata
     7501 tgatttctat tggtacaaaa tcaaattaaa accatgaaaa aagaacaaaa taaaacattg
     7561 aaaagcatat acatttgggt acttgtagcc aatgctcttt atattattct gttttatatt
     7621 tttatgaaat acttttctta aatcatgcaa acactcgatt ggtttatact cattttttcc
     7681 ctactattta tagtggtgta tggagcatgg aaaacgaaag gtagccaaga tgtaaaagat
     7741 tatattttag ggaataacga gagtaaatgg tggacgattg gtatttccgt aatggcaaca
     7801 caagccagtg ccattacttt cctgtcgaca cccggacaag cttttcatga cggaatgggt
     7861 tttgttcaat tctatttcgg attgccgcta gcgatggttt ttatttgtat cttttttata
     7921 cctatttatc ataaactcaa tgtttatacg gcttatgaat ttttagaaaa tagatttgat
     7981 cagaaaacac gaagtttagc cgctatactt tttttagttc aaaggggttt agcagccgga
     8041 attaccatat atgctccggc aattattttg tcttctattt tgggttggaa tttaaattgg
     8101 cttaatttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct ccggcaatta
     8221 ttttgtcttc tattttgggt tggaatttaa attggcttaa ttttattatt ggtgttttag
     8281 taattattta tacggtttcc ggcggtacaa aagcggtgaa tgttactcaa aagcaacaga
     8341 tgtttattat tatgtttggt atgtttattg cattctattt aattttaagc tatttaccaa
     8401 aagaaatctc ttttagcaat gctctccaaa ttgccggagt aaatgataaa atgaatattc
     8461 ttgatttttc aattaatccg aatagtcgtt atactttttg gagcggaatt acgggtggat
     8521 tatttttagc tctctcctat tttggtacag atcaatcaca agtgcaacgt tatctttcgg
     8581 gaaaatcagt tcgggaaagt caacttggtt tgatttttaa cggttttcta aaagttccga
     8641 tgcaattttt tattttgttg accggcgtaa tggtgtttgt tttttttcag tttaataaag
     8701 tgccactaaa ttttaatcca accagtcaaa ttgcggttga aaaatctatt tatgctgctg
     8761 attataaaaa tttagaagaa cgattagcct tgattcaaga ggagaaaaaa attgtgacct
     8821 taacgtatgc caaacaaata aatattgatg ttgataatcc tgtgctgaga aaaagaataa
     8881 ttgctttatc tgagaaagaa aaaaacttac gggaagaagc acaaggcatc ataaaaaaaa
     8941 tcaagcagga acccaatgac aaggattatg tttttatcca ttttatatta cattatctac
     9001 cgacgggcct tgttggttta ttacttgctg taatactttc ggcagctatg tcgtcaacgg
     9061 cttctgaatt gaatgcgttg gcttcaacta cgaccattga tatttataaa cggaatttaa
     9121 aaagtgagaa gtccgacaag cattttgttc acgtctcaaa agtttttacg cttttgtggg
     9181 gagctattgc gattttattt gcttgttttg gaacgctggt agaaaactta attcaattag
     9241 tgaatattat cggttctgtt ttttacggaa ccattttagg aatattttta gttgcctttt
     9301 ttgtaaaatt tattaaaggc aatgctattt tttatggagg agttctcagt caacttatca
     9361 tttttatcat ttactatttt accattcaca tttatccatc gggtaaagaa ctcttaggct
     9421 atttatggct caatgcgatt ggtgctgcac taactttagg attgtccgct ttgattcagg
     9481 ttagtatgaa aaataaaaac taaattaaat ttaaggaaat aaaaaaatcc tatactgaga
     9541 attcagtata ggatttatag tattcaaacc ctattatttt ttactccact cggaaatact
     9601 gtctttgttc atttttacgt aatctgcatt acccgctttt tcagcagctt ctaacgatac
     9661 tttagccgtt tcgatagctc cttttttatc gcctaattta gcttgaattt gagattttaa
     9721 acgattatac caaaaaggag ttcctttttc agcggtcatt gcaactgctt tatttacata
     9781 ttccaaggct ttagtcatat cgccatttga ttggaaaaag tactgtgctg aagaaaaata
     9841 atctccagca gttggtcctg ctaacacttt atcaatgctt gccatggcta atttgcttgt
     9901 tggaacttca aatttaaggg caaccaatgt tttttcccaa gacaattcta agtgagcaaa
     9961 atcatttgca atattattca aggaaatcgt gaacgtttcc acatttctgg ttaaggtttc
    10021 cggctttaca ttggtacgta aggcaacttt agcttcatcc cagttttcag gagttcccca
    10081 attttcagta tctgcataaa ataccacttc ccaattatca gcttttggcg ttgtgaaaat
    10141 ggcatatttt ccttgtttca atgttttacc atcaataaca acatcatctc caaatgttat
    10201 cattgaattt tgatttgcac cggttctcca catttttcca aaaggaacta aatttccgaa
    10261 taccgctctt ccttttgcac tcggacgact ataatcgatt tcaacctctg ttaatccgac
    10321 tgattgttta aaattagctc tcggacttgg agctggtgtt ttcacttgtg ctcccactgt
    10381 cattgtagct acaaaaaatg taaatgctaa aactattttt ttcatgtttt aaaaggtttt
    10441 gttaatttat caaatttaca gtttcatttt ggcactaact gttaagaaaa gtataaattg
    10501 aaattatttt gtttaatgtt tcgatattta atttaaacaa tattgtacct ttacaaaaaa
    10561 atatagcatg aaaatatata cgttacatac gaaacaaaac ttacctattt cattagaaga
    10621 ggcatgggaa tttctttcta gtccgaaaaa tttaaaaaca ataaccccag attatatggg
    10681 tttcaatatt ctgtcagggg ctgaaaaacc aatgtttccg ggtcaaatta ttcaatatat
    10741 tgttactcca atattaggga ttccaacaaa atgggtgact gaaatcacgc atgttcaaga
    10801 caaaaaatac tttgtagatg agcaacgatt tggtccttat tcactttggc atcacaaaca
    10861 ttttttgaaa gaaattccgg gtggtgttga aatggaagat attgtggatt ataaaattcc
    10921 aatgggtatt ttaggtcaac ttgttcatcc gattttagtt aaaccaaaat tgaatgagat
    10981 atttaattat cgtcaaaaaa aattaattga actctttgga gaatataaag gataatatga
    11041 aaaacatact attgataggt ggctcttacg gaataggact ggccattgca aaagaattac
    11101 aatatgaatg taaaattttt attgctgcca gaacgaatga attgagcagt gatttgaata
    11161 ttacctatat tccatttgat gccacgacgg atgaatttga tactacaaaa ataccggccg
    11221 ttttagacgg attagtttat tgccccggga gtattaattt aagacctttt aagggaatca
    11281 aaccggaaac gtttgaaagt gatttaaatt taaattttat tagtttagtg aaggtgattc
    11341 aaactgtttt acctaattta atagcttcag aacaatcaag cattgtgctt ttcagtagtg
    11401 ttgccgccag tatgggaatg ccgtttcaca cgagtgttgc tgcttcgaaa ggagctattg
    11461 aaggatttgc aaaagcattg gccgcagaat atgctcctaa aattagagtg aatgtgattg
    11521 ctccttcgtt gacggacact ccgcttgctg ataaattttt gaacaatgat gttaaaaaag
    11581 aaaaatcggc ggaacgtcat ccgctgaaac gatttggaaa accggaagat ttggctcaaa
    11641 tggctcagtt tttattgagt gataaaagca gttggattac cgggcaaatc tttcatgttg
    11701 atgggggaat gtctacgctt ttggtttaaa gttttttaag atagtttacc gatgaatata
    11761 ttttggtttc gaagagattt acgattggat gataatgtag gcctatttca tgctttgaat
    11821 agcacggaag aagttttgcc tgtgtttata tttgatgaat ccattttagc tgaacttcca
    11881 aaagatgatg ctcgggttac ttttattcat gaattacttt ctaaaattca agagcaatta
    11941 gcccttcaag ggaaatcgtt agccgttttt cacggaaaac cgattgcggt ctttaagcaa
    12001 ttgattagcg agcattctat ctcgacagtt tataccaaca atgactatga accttatgcc
    12061 agaaaaaggg ataaagaaat taatcaattg ttgaaagaac atggtattga ttttaaaacc
    12121 agtaaagacc aagtcatttt tgaaaaaagt gaagtagtga aagacgatgg tttgccttat
    12181 gtggtttata ctcccttttc aagaaaatgg aaagagaaat tcaaaactat ttctctgccc
    12241 aattatcctt cagaaaatct attggataaa atagcccttc actcctatcc ttttttgagt
    12301 ttaacggaca ttggatttga aacttcctcg ataaaaatta ctccttttga tgtttctgac
    12361 aaactcgttg atcagtatga atcaactcgg aatttcccgg caatgtcggg aacttcaatg
    12421 attggagctt atttacgatt tggagctgtt tctattagga aaatggttgc gaaagccatc
    12481 gcttcaaaaa atgaaacctt ttggaacgag ttgatttggc gtgagttttt tatgcagatt
    12541 ctatggcact ttccgcacac tattcataaa agtttcaaag agaaatatga tgccattcaa
    12601 tggaataaca atgaaacgga atttcaaaaa tggtgtgaag gaaaaacagg ttatcctatg
    12661 gttgatgccg gcatgcgaga attaaatgca accggacata tgcataatcg ggttcggatg
    12721 gtggttgcca gttttttatg taagcattta ttaattgatt ggcgttgggg agaaacctac
    12781 tttgctttga aactatttga ttatgagcaa gccagtaatg taggcaattg gcaatgggca
    12841 gcgggttccg gggtggatgc agcaccttat tttagaatat tcaatccggc ggagcaaatt
    12901 aaaaaatttg ataaagattt aaagtatatt aaaaagtggg ttcctgaggt tgaaacctct
    12961 gcttattcct taccgattgt tgatcacaaa gaagctcggg aacgttgctt gcgggtttat
    13021 aaagaagccg ttggataaat ttatttaaca caatttggga aatatcaatc ataattaatc
    13081 gtaccttaca gaaaatcaaa tagataaatt atgaaaaaca gattaattat gttgactatt
    13141 ctagcaacag caacattatt ttcgtgtgct aaaaaagaag aaaaaccggc agaagtgatt
    13201 gttattgaac aacctgctga acaagcagct cctgttgaac aacctgctcc tgttgaaaat
    13261 accgacggta ctacaattga agttgatgaa aacggcttaa aagtaaattc tgaaaaaact
    13321 tctgtcgata ttaaagatgg taaagcaaaa gtagaagtaa aaaattaagt tattatagaa
    13381 tttccctctt ccggagggaa attttaattt atcaattgta tcttttcaaa actcaattcg
    13441 ttgaagtgac taattacttt gtctgccaat gtataatctt gtagtttaga gtttacacta
    13501 tcatatccga tgcaataaat tcctgcggct ttcgctgcta aaattccgtt tgtactatct
    13561 tcaattacta tacagttttc ggttggggtt ttggctaaaa aagcagcttg ccggaaaatg
    13621 gctggatgcg gttttgaatt aggaaaatct tctccggaaa ctttgtgcgt gaagtattta
    13681 tgcaaatcaa aacggttgaa aatcctttca atagttacat ttgctgaaga agaagctaag
    13741 actaactgca tttcattttg atgtaattct tttattagtt cttctactcc gtctaataaa
    13801 tagatgtctt ttttttgatc gaaggcatcg ttaaataagt tgcgttttac ttctactaaa
    13861 gttttgacat cttgttgtaa gttataaact gattttaatc gttcataaat atttttggtt
    13921 gaatttcctg tgaatgaagc atacatttca ggagaaacgt cgatgtttag ttgtttgaaa
    13981 tgttgttgat aggcgaaatg atgtaccggt tcggtgtcta caattacgcc atccatatcg
    14041 aaaattactg tttgtatcat aaggggctga gttgctgaat tgttttgctt tatttgttaa
    14101 ttttttagaa cacagatttt agaaattaaa ataaaaataa taacccattg agtgtaattt
    14161 aattacagtt ttaaccacat agaaacatag ttttttatag attttgaaag tcgtttcact
    14221 tagttataga aaatcatagt tatactttac taattataac caactgatta ataataattg
    14281 ccttttaact cttttccaac aaatatttta ctacagcttc tgctgcatag agtccgaaaa
    14341 ggccgggcat ccaactgttt gttccgtaga atgatttttt gaaattattt ccgtctgtta
    14401 gttttaaact tgattcgtct tggatttctg aggagaaaac aactttcagt ttgtcaattt
    14461 ttacttcttt taaacgtcgt ttcattgttt tggctaaaaa acagttttca gatttactga
    14521 tgtcggagac ttttactttt gaagcttcca tcttccctcc tgctcccatg ctggagatta
    14581 ttttgactct ttttcttttg gccgctatga ttaaatttaa ttttggggtg atgctgtcaa
    14641 tgcaatctaa gacatagtca aattcttctg aaacgatttc cattgctctt tcgggagata
    14701 aaaattcttc tacgcgaact aaatttaatt ccgggttaat gtccattaat cgattgccaa
    14761 cgattgttac tttgggcatt cctattgttg aatgcaaggc aggtaattga cggtttatgt
    14821 ttgtgatgtc gactacatcg ccgtcgacaa tggttatgtt tcctactccg gcacggacta
    14881 agaattcggc ggcgaaagag ccgactcctc ctaatcctac gactaagacg ttagcgtttt
    14941 ttaatttgtt taatccttct gttttaaata agagctgtgc tcgttcttgc catttagtca
    15001 tttttctttt gttgtggttt gttgttaatt atgagattcc tccttcgtcg gaatgacaag
    15061 actgggggga aatacctttt caaaatttct tataacgatt gacttcattt gttttatttc
    15121 ggaatgacaa gactacggga aaatactttt tcaaaattgc ttgcgatgat tgctttcatt
    15181 tcttctattt cacaattttt atactttgct gctaaagcat atacttcttc taatgattct
    15241 tcgatggtgt cggtttcgag gaaaaattta tcgtttggaa tggattgaaa tacggttttt
    15301 aattccgggt ttctgattaa atattttcca aatgagagat agaatccact gtttatcaat
    15361 tgttttgcgg tttgttcgtt ttttgaaaat ccgtgtacaa tcattggtac agttatcttt
    15421 actcgttttt ttatttcaat tagttcctga aaagcggaaa cgcaatgcaa aactaatggt
    15481 ttttggtgtt tttctgctaa actaatttgc ttttcgaata cttcgatttg aagttgtagc
    15541 ggaatgtcga ttcgtttgtc taatccgcat tcgccgaggg cgaggcattc gggtaatgcc
    15601 agtttttcgt caatcttttt taaatcttgt tgcaaccttt gttcgtctat gtaccaagga
    15661 tgtattccga tggaataggt gggaatggat tcgtcaaatt cccatgggta ttggttgacc
    15721 aattccaatc ttgtttgttg gttagtaaat tggtgggtgt gtaaattgaa atagttcatt
    15781 agtcgtggta ctttttgact ccggctttta cttctacttt taaatcattt tctgcgggtg
    15841 gaattgggca agaatatttt tcattgtagg cacaataggg attatagcct ttgttgaaat
    15901 caatggtcca ggtgtttcct ttttgtactt ctaggtcgat gtaacgtccg ccgccgtagc
    15961 tttcgactcc ggaagtgagg tcggtgaagg gaagaaacaa gctgtcttga tgccccggtt
    16021 tcttggcata ttctacattt tgatagacgt ttagtttgca gggaacgtcg ttgatggtga
    16081 attgtaattc gccatatttt acatagatgg gttttcgggc tgtggtggtt ttcatttcaa
    16141 atggtttttc cttttttgtt ctggtgaatt ttgcttgaac aaaataggtt tctgaaattg
    16201 ggtaaaactc taagttcacg aagtgtttta agtcttcttt ctttaaaggg cttgatgcgg
    16261 tatcggcata gtgctcgttt agttcttttt gaaattgaag aacggaatct ttttcaaatt
    16321 tttgagcgta agagagttga gcgattaaga ggagaaagag aattttcatt aataaattac
    16381 aaattatgaa ttacgaatta cgaattagtg tttggttatt ggttgtttca aatttaatta
    16441 attggaatgg aattatgggg gattttaaag gatttaatag gttttttgtt gaaggattga
    16501 acacggatga ggtggattga atggatcccg aagtctcggg acggattttt attgggtatc
    16561 gctttgcgat aaatggattt taaaggattt actacgtttt tgtttgaagg attgaacacg
    16621 gatgaggtgg attgaatgga tcccgaagtc tcgggacgga tttttatagg gtatcgcttt
    16681 gcgataaggg gattttaaag gatttccaat gtgagtggat tgcttttgtt tttttttaat
    16741 tctttaaatt ttcagttttt gagattcaac gcagatgaca aggtattatt tcaacgaagt
    16801 tggtagactt ggtggtgatg gtttttatta ataatcaaaa gtcaattc
//
LOCUS       DAOTCW010000045        16737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_1941_length_16737_cov_44.258182, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000045 DAOTCW010000000
VERSION     DAOTCW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 16737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16737
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1941_length_16737_cov_44.258182"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="P5335_05420"
     CDS             <1..1530
                     /locus_tag="P5335_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRZ74352.1"
                     /translation="ESNAISIALQIQTPVLSAVGSLCGTNSVQLNSSVTSNVFTYKWY
                     KNNTLITGASLPSYSTNQAGNYHVVVSYGTCSVTSNILNLQQNSITATLNLPSTNLII
                     PGETKTITVTTNAVNPSFQWFRNNAIITGATTASLNTTENGAYKVIVTQTTGCNATQE
                     AITSLIYPTSFNVTIAANANYSSCNSTQATLSISSFNAVTSTETVSIVNNSFGYVYEW
                     YKNGALVSSSVNNTYLVASNTDNGTYELRVVIPNFNNVISNELPIQLGIIEDFVLSSE
                     GVLCETGGTVILSSNITNSNYILAWYANGNDTVLGTENTLLVNNPGEYFMTIEFEGCT
                     YTTNSIAVNVLSSDGITLNIADSITVIQGTSTEVIATGAENYTWYFNSQNIGTENSIS
                     VSQAGIYTVIGTIGDCEFTKTFTVTVVENTSIVVPNVISRNNDGINDFWSIPNEYVNK
                     EEVEVIIYSPKGKVIFRARNYQNNWPNNTLEYSKKEPVFYYTISNNDEIIKRGSITVI
                     E"
     gene            1534..3525
                     /locus_tag="P5335_05425"
     CDS             1534..3525
                     /locus_tag="P5335_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRZ74353.1"
                     /translation="MKNYVFAILFSLVLFADLQAQDGPVLTFTIPTQNNLLLNRFLIN
                     PTFSAVKESDSFITLYHRNQWIQFDDSPELYMLSYSGKYGERAGVGIGIYQQNLGIIT
                     SFGGIGNYAYEIPLKEKMSLTLGFNLAYYTSGVNRNKAVTNEPDPAILALRNNSVLTL
                     KPGINFSWGSFDMGIYAENLVDYDFKSSEMVKEYSEKIYTSHVVYNHQMQNQEGLFDE
                     GYFRLMLRGRLSQSEDFQYGGSIMVNFPKLGWVQAGVDDFFGVGIGAGAHLTKKLSLC
                     YTYERVINEGLVNFGPTHEITISYRFAGKENEQKNNYKSAPKKPTASINRNQKQAKSN
                     IINEEEEVEEDSVTEEYEDLAEEPIVVEPATKRKKSVTKKNTATTSNKTTSTDLDEGE
                     SVSKTFDKNIEFEKLRMTLDENNLKLLEIIVKQDSIENLKKADFEKRIENMMHYIQRL
                     ETTITEKDCNCEPKSTVVTEEKTLETTTTKTTVKSPERAIIKNEKTDLNSKLNAMKSS
                     SKKEEIKLPITKKNTVETELENKIITLTKSKNDESKTNTNTKFSLSDEEIKAYYSRLT
                     DKKRATAKKGNFLEVENQEPGFYIIANVFSEPAFADDFLKKLKKQGIKASYFINPKNN
                     FRYVYLKKHSEWSEALISYYTNVDNTYFEEIWIMNINIK"
     gene            3537..>5728
                     /locus_tag="P5335_05430"
     CDS             3537..>5728
                     /locus_tag="P5335_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HRZ74354.1"
                     /translation="MKTPQIPHLKTIALLSCFLLINLIGTAQVAVPFTVRYERNLKGD
                     MTLISNNIVNRQNNNDGPNIPYDVTGNSSEYNDNLNMQYIDVDGDASTFSSSSATLTL
                     PASGCNKIVYAGLYWSATYRFNNGFSTSPGDGDNVRANDFNQIKLKLPGGAYQNITGQ
                     LIFDGFTDPNFIANSPYACYANITSLVTGLANPEGEYTAANIRATQGFVNGGGVSGGW
                     TIFFVYENPTMTGKYITAYDGFAGVRATIGQTDISYSGFTTLPPPFPVRAKLASAALE
                     GDNRITGDQLLFKAATNPTFTALSNTLNVANNFFNSRITLNNLDFTDRVPNSLNTLGY
                     DGDIITISNPMNSVIPNNETAATLRITSTQDTYFMFFNAFNIEVIEPDIKLVKTVQDI
                     SGNDIGGANVNLGQILDYVITFQNIGNDDATNSSIRDILPINTTFISADLSGAPGVTF
                     THDPSTDELFFTLPDNLLQIGDPIYTIRIRVQVATTCSELEDACSNLIQNQAYTTYQG
                     VLNSNVISNDPSFAGLDSCGFGLPGPANFLVDIDDCIFSSDAYLCGTSLLLTAANGYD
                     TYTWYNSAGTVIGNSQSITVNTVGTYTVVNTATPPCIGITQTFNVLLFGATQTNPVIP
                     FADQVVICPNNGEQLPLIFLCGANDSQLIQTGISDATSIVWEILNESSCPAVGIANCA
                     NNNPTCQWNQVGTGANFNVTTAGQYRLTINYQDGCFTRFYFNVFTNLL"
     assembly_gap    5729..5828
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5829..>10748
                     /locus_tag="P5335_05435"
     CDS             <5829..>10748
                     /locus_tag="P5335_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74355.1"
                     /translation="QDGCFTRFYFNVFTNLLDIQFTSTNILCNTPGSITITNLPSDYQ
                     FQLIDQVTGAIIFPYQNSPVFTISQAGVYTVLVTQIGVANGCVFELPNIGIQQNNFQV
                     TLIPQNTNCNGFGSIRAQISGAQPQYTFTITNPISITSGLVTDNDYTFNNLNPGTYTV
                     TVTTEDGCTFTDTVTILDQNNLALVATVTQSIGCSSGLITVVANGGNPPYSYAISTID
                     GVAQNPTAGDFQTTDTFVITTPGTYTFIVVDANNCNSVSNPVTITISPDVVYTSAVQN
                     VLCNGASSGSITYTISNSNGFQVTFQLVNSNGVVIASNNSGIFTNLPIGTYTVNILQS
                     QGSTSCTFTDTFVITQPSPITGIAVITQNYTCLTNGSIGIQTGSVSGGTAPYLFSIDG
                     VNFSTISDFSGLTSGNYTIYIQDANGCLFTTNSISLLAPNQLTAITFSATALTCPSQV
                     STVTISTVGGTAPFNYQIIAPVGQTVNNGTNPVFTGLSSGTYTFLVTDSNGCSYQDSY
                     TINALPSIDVIGQTVSNVICFGGNNGEVLFTVNSTNPFNYQVTNTAGTVISSGNNSNL
                     TTISLAGITASTYTISVTDTVTNCTDSATAIINAPSTALAFTFTANQLSCASNGSVTI
                     STIGGWGSYQYQLTQPNGTILGPQSSSTFTNLTQTGTYTISVNDINGCIVSNTFNLAT
                     PTNPVAVIASSSDLCFDANGATLVIAVTGGVVPFSYSINGNPSQTSNTFANVTPGSYS
                     ILVTDANGCSTTVSQTIQPELTGEIIITKGLDCTSSPNAVIAATVSGGLAPYVFQVSF
                     NGGAFGTSNSIAGNTFIYQTANAGNYQFLITDAQGCSFTSSIITVDPLTQPEIISVSQ
                     IQNILCSGGNNGEIEININNSVGAAPFVLDVINTTTGVNYGTQTSGLTAGVYQITLTD
                     ENACTDTASITITDPAPITFTVSTVEITCNNPGGTSYGQIIVQGVSGGTAPYTYELHN
                     NFGFVASYTTTANENHSFQILNFGIYSVEVIDANGCSMIDANIIIASPPNDLIIDVST
                     STSDCINGGTAIITVTSAVSSGSFEFAILETNTLPYSSNYQSADVGTPETATFTGLTP
                     GVTYTFVVHDLITDCYYFKTADTPINTPSNLTSTLDVVGNVSCTGSNNGFVNFTFTNY
                     DVTSTGVSYEIYNSQSNTSTGITGTSTPLLGGPVSVTNLGPLAPGTYYILFTEIGGTL
                     TGCSSASASFTITQSANLLQVTANVTRNDNCNPNKGRITATGQFGTAPYQYQLVLAGD
                     PAPTVGTWVGSATNVFNVEGGNYVVYIKDANGCIQAAPVFVPTDPAPQISATVTNLCT
                     ATQGSFSIEVTLTSSGIAPYTFSLNGGAYQTQTVPTFTYTNLNSGNYNIRVRDYNGCI
                     HNVLISIPVPLNLTPAVTAQVSCANNDGIITITPFGGSGTFTYELQNTSGTVIVAAQS
                     SPVFNGLAAGSYIAIINNTTTGCSAQAPIVLEAPTPVTFTTSISDISCNGGNNGSIIV
                     SLPATNNNPMYTFVLTDGVNTPISQNNGNFTGLVAGTYTITVTSGRNCQLQQVVVINQ
                     PAVLTVSASATAFACNSSNVVQTATITASAAFGTAPYVYSINGTNFFSSNTFTIVDNG
                     SVQNITVTVRDANNCSATTSVNINPLPTI"
     assembly_gap    10749..10848
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10849..15078
                     /locus_tag="P5335_05440"
     CDS             <10849..15078
                     /locus_tag="P5335_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HRZ74356.1"
                     /translation="ANNCSATTSVNINPLPTITAVTASTVTPLTCTNNGTVQLNVTGG
                     SGDFTFDLLPLGSYPSVTPGIGIFTVNFTLTTPGTYTFQVTDNVTGCYFITAPYVIAP
                     FNTIQASIINSTAVTCFGDTNGTITMNVSGYSGNITYVVNDIQGNTVANGSGNTSINP
                     FVITGLSGGNYQVIVTATDSPFCSASSNFTVVSSPSLPLVLVANQTASVTCTNNQGEI
                     AASASGGWGTYQFQLVNNTTSTTVQPYASNSLFTGLSAGNYTISVQDAGGCVISSTII
                     LVQPSLISATLTATNTALLCFGDTNASVTATNVTGGQGVYQYFLNTYNASGTTIVSSS
                     GGQTSPTFNGLGAGIYSITITDGWNCDFTSNTVIITQPTPVVASLNLTSSLTCLNNAV
                     ITISASGGTAPYQYSSDGVTFSNNTTFNVGPGTYQYFVRDANNCAVVQTNQITINAIP
                     ALELNLNLNSATINCFGESTATIIANTTGGLGNYSYTLLDTANNVIAGPQNSGTFANL
                     PAGDYIVRVDSNDCNAVSNVIQITNPSQLLISALTVNNVLCNGGTNGSVVIQTSGGTG
                     TVQYAISPNLNQFFNTGTFNNLAPGNYDIIVQDQNGCFILLDVDIIEPAPLVADVANV
                     VQNLCLGDNDGSFDIVISGGTAPYSTSLNNQDPSAFVEGQLSFSGLVGGTDYFIFVKD
                     ANGCETIAYVFLNAPVEVIPTVDIVYNCTGNIPGNTVTVSVNSSAVGNVQYALDGGTY
                     QPSNLFQNVPTGNHTISVQHTNGCIKTADFIINANQPLAVSLANVQNVLCNSGNTGSV
                     TVATTGGTGTIEFAISPDLTNFSTNNTFSNLIAGTYTIIVRDGIGCSVSQQVIITQPT
                     LVTTSVSAIAQELCTNDGNGSITIAIAGGVAPYSTSLNNSSPGSFVLNQVTFSSLVGG
                     QTYTIYVLDANGCITSIPVTLDAPVTINPTVNVAYVCNGNTVGNSVTVSVNTAVQNDV
                     TYSLDGGTFQTSATFTNVAVGNHTIVVRHNNGCEKSVSFIINPSTPIQASFTSTNVTC
                     NGLTNGLIQVAASGGNGALEYAISPNLTSFSASSTFNNLAAGNYTVTVRDALGCSVTL
                     NITVTQPNALNASLVTMFPDLCYGDNVGAIEISIAGGTAPYSTSLNNENNYVVNQVLF
                     ENLSGGQSYTIYVRDTKGCTSTLEVAVSGGISINPGITITYDCFGSAQFNSVKIKVGQ
                     EISSQVTYSLDGGAFQNGNVFTNLTNGNHTITILHALGCSETTSFTIENYIPISLTLV
                     ESNLNQITATATGGSGSYTYYLNGVNYGDLNELQIFANGTYTVLVEDSNGCTAEAQIT
                     MDFIDIDIPNFFTPNDDGDNDTWVPENLEGFPNAKVEVFDRYGRIIVQFGTKGSWDGT
                     YNGSLLPTGDYWYTITLDGGRQFVGNVTLYR"
     gene            15150..16088
                     /locus_tag="P5335_05445"
     CDS             15150..16088
                     /locus_tag="P5335_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRZ74357.1"
                     /translation="MKKLAPFFILLAFLNGKAQELNLPAFTQYLADNPFVISPVYAGI
                     GDNIKIRANGLTQWVGIKDAPKNMSLAADMRIADRSGVGLYLYSDRNGNTRQSGAKVS
                     FAHHLVLDKYEDQYLSFGISYNLNQFRIEIENFTGQDAAVVDDRRLTNHNVDVGVLYR
                     INGFYISATAANILDKNIDVFNDIEPLELRNYQLFTGYKWRSNTNSDFEVEPSAFFQY
                     FESDKRSTTDLNLKMRFYNFDDYYWAGISYRFLNDQLLEPLNIGPMLGLKRSNFYFAY
                     SYQVTLNKLINYNSGTHMITIGLDVFQGISNCPCTY"
     gene            16101..16460
                     /gene="folB"
                     /locus_tag="P5335_05450"
     CDS             16101..16460
                     /gene="folB"
                     /locus_tag="P5335_05450"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRZ74358.1"
                     /translation="MGTIRLQNIRTFSFHGCLLEESKIGSDYSIDLEVKTDMRKSALS
                     DDLHDTVDYVLLNRIVVEEMAIRSKLLEHVAHRIISRIFKEIPSIARVLIGVSKLNPP
                     IGGDVEAVTIQMEEYRS"
     gene            16586..16656
                     /locus_tag="P5335_05455"
     tRNA            16586..16656
                     /locus_tag="P5335_05455"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16618..16620,aa:Cys,seq:gca)
BASE COUNT         5426 a         2921 c         2902 g         5288 t
ORIGIN      
        1 gaatcaaatg ctatttctat tgctttacaa attcaaacac ctgttttgtc cgctgtgggc
       61 agtctgtgtg gaacaaattc ggttcaatta aacagctctg ttacgagtaa tgtgtttact
      121 tataaatggt ataaaaacaa tacattaata acaggagcat cacttccaag ctacagtaca
      181 aatcaagcag gaaattatca cgttgttgtt tcatacggta cttgttcagt aacatcaaat
      241 atattaaatc tgcaacaaaa cagtattaca gcaacactta atttgccgtc aactaactta
      301 atcattcccg gtgaaactaa aacaattacg gttacaacga atgcggtaaa tccctctttt
      361 caatggttta gaaataatgc cattatcacg ggtgcaacta ctgcttctct taatacaact
      421 gaaaatggtg cgtataaagt tatagtcact caaactacag gttgtaatgc tacgcaagaa
      481 gcaataacat cactgattta tccaacgtca tttaatgtca caattgctgc aaacgcaaat
      541 tattcctctt gcaatagcac gcaggctact ctttctattt ctagtttcaa tgccgtaact
      601 tcaactgaaa cggtgtcaat tgtaaataat tcttttggtt atgtctatga atggtataaa
      661 aatggtgcat tagtttcatc atctgttaat aacacctatt tagttgcttc taatactgat
      721 aatggaacct atgaattacg tgttgtaatt ccaaatttca ataatgtcat ttcgaacgaa
      781 cttccaattc aattagggat tattgaagat tttgtgcttt cttctgaagg agttttgtgt
      841 gaaacgggag gaacagttat actttcatca aacattacaa attcaaacta tattttagct
      901 tggtatgcaa acggaaacga tactgtttta ggaacagaga acacattact tgttaataat
      961 ccaggtgaat atttcatgac cattgaattt gaaggctgta cctacaccac aaattcaatt
     1021 gctgtaaatg ttctttcatc tgacggaata acattaaata ttgctgattc gattactgta
     1081 attcaaggca cttctacaga ggttattgct actggagcag aaaattatac ttggtatttc
     1141 aatagccaaa acattggaac cgagaacagc atttcagttt ctcaagcagg aatatatact
     1201 gtgattggaa ccattggcga ttgtgaattt acaaaaacat ttaccgtaac cgtggttgaa
     1261 aacacaagta ttgtggttcc aaatgttatt tcaagaaata atgatggaat aaatgatttc
     1321 tggtctattc cgaatgaata tgtaaacaaa gaagaggtag aagtaattat atattctcca
     1381 aaaggaaaag tgattttcag agcacgtaat tatcaaaata attggccaaa taacacttta
     1441 gaatattcaa aaaaagaacc tgttttttat tataccattt ctaataatga tgaaattata
     1501 aaacgtggtt caataactgt tatcgaataa atgatgaaaa attacgtatt cgccatacta
     1561 ttctctctgg ttttgtttgc agatttgcaa gcacaggatg gacctgtttt gacgtttacg
     1621 attcctacac aaaataattt gctcttgaat cgttttttaa tcaatccaac tttttcagcg
     1681 gttaaagaat ccgattcctt tatcacatta taccatagaa atcaatggat tcaatttgat
     1741 gattctccgg aattatacat gttatcctat tccggaaagt atggcgaaag agcaggagta
     1801 gggattggta tttatcaaca aaatttaggc attattacca gttttggtgg aattggaaat
     1861 tatgcttatg aaattccatt aaaggaaaaa atgtcgttaa cccttggttt caatttagct
     1921 tattacacta gtggtgtaaa ccgaaacaaa gccgtaacga atgaacctga cccggcaata
     1981 ttggcgttgc gaaataattc ggtattgact ttaaaaccgg gaataaattt ttcatgggga
     2041 agttttgata tgggaattta tgcagaaaat ttggtggatt atgattttaa atcgagtgaa
     2101 atggtaaaag aatacagcga aaaaatttat acaagccatg ttgtatataa ccatcaaatg
     2161 caaaatcaag aaggtctttt tgatgaaggt tatttcagat tgatgttacg aggaagacta
     2221 agtcaatcag aagattttca gtatggtggt tctataatgg tgaattttcc aaaactggga
     2281 tgggtacagg ccggagtgga tgatttcttt ggtgtgggta ttggtgccgg tgctcattta
     2341 actaaaaaat tatcgctctg ttacacctat gaaagagtga ttaatgaagg gctggtgaat
     2401 tttgggccaa cacatgaaat tacaatttct tatcgatttg caggaaaaga aaatgagcaa
     2461 aaaaacaatt ataaatcagc tcctaaaaaa ccaacagcat ctataaatag aaatcagaag
     2521 caagcaaaat caaatataat aaatgaggaa gaagaagttg aggaagatag tgtaacggaa
     2581 gaatatgaag atttagccga agaacctatt gtagtggaac cggctactaa aaggaaaaaa
     2641 tcagttacta aaaaaaatac agcaacaact tctaataaaa caacttcgac cgacttagat
     2701 gagggagaat cagtttccaa aacatttgat aaaaacatcg aatttgaaaa attgagaatg
     2761 accttagatg aaaacaattt aaaattattg gaaatcattg taaaacaaga ctctattgaa
     2821 aatttgaaaa aagccgattt tgaaaaacga atagaaaaca tgatgcacta cattcaacga
     2881 ttagaaacaa ctataacaga aaaagattgc aattgtgaac caaaatctac agttgttacg
     2941 gaagaaaaaa cattagaaac aaccactaca aaaacaactg taaaaagtcc ggaaagagca
     3001 attattaaaa atgaaaaaac tgatttaaac agtaaactga atgccatgaa atcttcttct
     3061 aaaaaagaag aaattaaatt acccattacc aaaaagaata ccgttgaaac agaattagaa
     3121 aataaaataa taactcttac taaatcaaaa aatgatgaat caaaaacaaa tactaacact
     3181 aaattcagtt tatccgatga agaaatcaaa gcgtactact ccagattgac agacaaaaaa
     3241 agagctaccg ctaaaaaagg aaacttttta gaagtagaaa accaagaacc cggattttac
     3301 attattgcta atgttttttc tgaacctgct tttgcagacg attttcttaa aaagttaaaa
     3361 aagcaaggaa ttaaagcaag ttattttatc aacccaaaaa acaatttcag gtatgtctat
     3421 ttaaaaaaac attcggaatg gagtgaagcc ctaatctctt attatacgaa tgtagacaac
     3481 acctactttg aagaaatttg gattatgaat atcaacatta aataaaaacc aaaaaaatga
     3541 aaacgcccca aataccacat ctaaaaacaa ttgccctact cagttgtttt ttactaatca
     3601 acttgatcgg aaccgctcaa gttgccgttc cttttaccgt tcgatatgaa agaaatctaa
     3661 aaggagacat gactttaatt tccaacaata ttgttaacag acaaaacaat aatgacggac
     3721 ctaacattcc ttacgatgtt accggaaatt cttccgagta taatgacaac cttaacatgc
     3781 aatacattga tgttgatggc gatgcttcaa cattcagttc aagtagtgcc acgttaacat
     3841 tacctgcaag cggttgtaat aaaatcgttt atgccggttt atattggtca gcaacctatc
     3901 gtttcaataa cggattttca acttctccgg gagatggcga taacgttaga gcaaatgatt
     3961 ttaatcaaat aaaattaaaa ctgccgggag gagcttatca aaatattacg ggtcaattaa
     4021 tttttgatgg atttactgat ccaaacttta ttgctaacag tccatatgcg tgttatgcaa
     4081 atattacttc attagttaca ggtttagcaa atcctgaagg agaatatact gcagccaaca
     4141 tcagagccac acaaggtttt gtaaatggtg gtggtgtttc cggtggatgg actattttct
     4201 ttgtatatga aaatccaacc atgaccggaa aatatattac cgcatatgac ggatttgcag
     4261 gagtaagagc aactatcggt caaacagata tttcctattc aggttttaca actttaccac
     4321 caccatttcc ggttagagca aaattagctt ctgccgcttt ggaaggtgat aacagaatta
     4381 caggcgatca attgttgttc aaagcagcaa caaacccaac atttaccgct ttaagcaata
     4441 cgttaaatgt ggcaaataac ttttttaaca gtagaattac actaaataat ttagatttta
     4501 cagacagagt tcccaatagt ttaaatacgt tgggatatga tggtgatatc atcaccataa
     4561 gcaacccgat gaacagcgtt attccaaata acgaaacagc ggctacgtta cgaattactt
     4621 caacacaaga tacttacttt atgttcttta atgcgttcaa cattgaagtc atagaaccgg
     4681 atattaaatt agtaaaaaca gttcaggata taagtggtaa cgacattggt ggggctaatg
     4741 tcaacctagg gcaaatcttg gattatgtga ttacttttca aaacatagga aatgatgatg
     4801 caacaaattc atcaattcgc gatattttac ctatcaatac aacatttatt tcagccgatt
     4861 tgagtggagc tccgggcgtg actttcactc atgatccatc gactgatgaa ttgtttttta
     4921 cgctacccga taatttattg caaattggtg accctatata taccattaga attcgtgtac
     4981 aagtggcaac aacttgtagc gaattagagg atgcgtgttc taatttaatt caaaatcaag
     5041 cttataccac ttatcaaggt gtattaaata gtaatgttat ttcaaatgac ccaagttttg
     5101 ccggcctgga tagttgtgga tttggtcttc cgggaccggc gaatttcttg gtagatattg
     5161 atgattgtat tttttctagt gatgcttatt tgtgcggtac cagtttgctt ctaactgctg
     5221 ccaatggcta tgacacttat acttggtata atagtgccgg aaccgttatt ggaaactcac
     5281 aaagtattac agtcaataca gttggaacat atactgtagt caatacagct acacctccat
     5341 gtattggaat tacacaaacg tttaatgtat tgctatttgg agctactcaa acaaatccgg
     5401 tgattccttt tgccgatcaa gtagtaattt gcccaaataa cggggaacaa ttacccttga
     5461 ttttcctttg tggagcaaat gatagtcaat taattcaaac aggaatcagt gatgcaacca
     5521 gtatcgtttg ggaaatctta aacgaaagca gttgccctgc tgttggcata gccaattgtg
     5581 ccaataacaa tccgacttgt caatggaatc aagtaggaac aggagcaaat tttaatgtaa
     5641 cgactgcggg acaatatcgt ttgacaatta attatcaaga tggctgtttt acccgatttt
     5701 attttaatgt atttactaat ttattagann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnat caagatggct gttttacccg attttatttt aatgtattta ctaatttatt
     5881 agacattcaa tttacttcta cgaatatcct ctgtaatact cccggaagta tcactataac
     5941 aaatcttccc tcagattatc agtttcaatt gattgatcaa gtgacgggag ctataatttt
     6001 tccttaccaa aatagtccag tctttacaat atcacaagct ggtgtttata ctgttttagt
     6061 aactcaaatt ggtgttgcga atggttgtgt ttttgaattg ccaaatattg gtattcaaca
     6121 gaataatttt caggtaactc taattccaca aaacacaaat tgtaacggat tcggaagtat
     6181 tcgggcacaa atttctgggg cacaacctca atatactttt actattacaa atccaatatc
     6241 tataaccagt ggattagtaa cggataatga ttatactttt aacaatttaa atccgggaac
     6301 atatactgtt accgtaacaa ctgaagatgg ttgtactttt acagataccg ttaccatttt
     6361 agatcaaaat aatttagctc tagtggctac tgtaactcaa agtattggtt gtagctcagg
     6421 tttaattaca gttgttgcca atggtggaaa tcctccatat tcatatgcaa taagtactat
     6481 tgatggtgtt gctcaaaatc caacagccgg tgattttcaa acaacggata catttgtgat
     6541 taccactcct ggaacttata cttttatcgt cgttgatgca aacaattgta attcggtttc
     6601 aaatccggta acaattacaa tttcccctga tgtggtttat accagtgctg ttcaaaatgt
     6661 tttatgtaat ggagcatcaa gcggctcaat tacttacaca atttctaatt caaatggttt
     6721 tcaggtaaca tttcaacttg ttaattcaaa cggggtagta attgcaagta ataactccgg
     6781 aatatttacc aatcttccta ttggtactta tactgtgaat attcttcaat ctcaaggaag
     6841 tacttcttgt acatttactg acacctttgt gataactcaa ccttctccaa ttacaggaat
     6901 tgcagtaatc acacaaaact atacttgttt aacaaatggc tcaattggta ttcaaaccgg
     6961 aagtgtttcc ggcggaactg caccgtatct atttagtatt gatggtgtta acttttcaac
     7021 tatttcagat ttttcaggat taacttcagg aaattatacc atttacattc aagatgcaaa
     7081 tgggtgtttg tttaccacaa attccatatc attactagct ccaaatcaat taacagctat
     7141 tacatttagt gcaactgcct taacttgtcc gtctcaagtt tcaacagtta caatatccac
     7201 agttggtggc acagctcctt ttaattatca aatcatcgct ccggttggtc aaactgttaa
     7261 taatggaact aatccggttt tcacaggatt atcttcaggc acttatacct ttctagtaac
     7321 agattctaat ggatgttcct atcaagattc atacactata aatgcattgc cttccataga
     7381 tgtaatcgga caaaccgtaa gtaatgtcat ttgttttggt ggtaataatg gtgaggtatt
     7441 gtttactgta aacagtacaa atccatttaa ttatcaagta acgaataccg ctggtacagt
     7501 tatttcctct ggaaataatt caaatttaac tactatttca ttagcaggaa taacagcatc
     7561 aacgtatact atttcagtta ctgacacggt tacaaattgt accgatagtg ctaccgctat
     7621 tattaatgct cccagtaccg cattagcttt cacatttaca gcaaaccaat taagttgtgc
     7681 ttcaaacgga agtgtaacca tttcaacaat aggtgggtgg ggaagttatc aatatcaatt
     7741 aactcaacca aacggaacta ttcttggtcc acaatcaagt tcaactttta caaatttaac
     7801 acaaaccgga acgtatacca tttcggtaaa tgatatcaat ggatgtattg tgagcaatac
     7861 atttaactta gctacaccca ctaatcctgt tgctgtaatt gcatcgtcat ccgatttatg
     7921 ttttgacgct aatggggcta cattagtaat tgcagtgaca ggtggtgtag ttccttttag
     7981 ctatagtatt aatggaaacc cttcgcaaac ttcaaatact tttgcgaatg taacgccagg
     8041 ttcttatagc attttagtga ctgatgcaaa cggttgttct accactgttt ctcaaactat
     8101 ccaaccggaa ttaacgggag aaattatcat aactaaaggt ctcgattgta cgtcttcacc
     8161 aaatgctgtg attgctgcaa ccgtttccgg aggtttagct ccttatgtct tccaagtatc
     8221 ctttaatgga ggagcatttg gaacatcaaa ttctatagca ggtaatactt ttatttacca
     8281 aaccgcaaat gctggaaatt atcaatttct aattaccgat gctcaaggtt gttcttttac
     8341 aagttcaatt attacggttg acccattaac tcaacctgaa attatttctg tttcacaaat
     8401 tcaaaacata ttatgttccg gtggaaataa tggagaaatt gaaattaata ttaataattc
     8461 cgttggagcg gctccttttg ttttagatgt aatcaacact acaacaggtg ttaactatgg
     8521 cacacaaacc tctggtttaa ctgcaggtgt ttaccaaatt acattaaccg atgaaaatgc
     8581 atgtactgac acagcttcca ttaccataac cgaccctgct ccaattacat ttactgtttc
     8641 aacagttgaa atcacctgta ataacccagg aggaacatca tacggtcaaa ttatagttca
     8701 gggagtaagt ggaggaacag ctccttacac gtatgaatta cataataatt ttggctttgt
     8761 ggcaagttat actactacag caaatgaaaa tcattcattt caaatattaa attttggaat
     8821 atattctgtg gaagtaattg atgcaaatgg ttgtagcatg atagatgcta atattattat
     8881 tgcttcgcca ccaaatgatt taattattga tgtttcaacg tcaacttctg attgtataaa
     8941 tggcggaaca gcaatcatca cggttacctc tgctgttagt agtggttcat ttgaatttgc
     9001 tattttagaa actaatacat taccatattc ttccaattac caatctgcag atgttggcac
     9061 tcctgaaacg gcaactttta caggcttaac tcctggagtt acgtacacat ttgttgtgca
     9121 tgatttaatt acagattgtt attattttaa aacagctgac acgccaatta atacgccatc
     9181 taacttaact tccacattag atgttgttgg taatgttagt tgtaccggaa gtaataatgg
     9241 atttgtgaac tttactttta caaattatga tgtaacatcc acaggggttt cttatgaaat
     9301 ctataattca caatcaaata cttcaacggg aattacagga acttctactc ctcttttagg
     9361 aggacctgtt tctgttacaa atcttggccc cttagctccg ggaacgtatt atatattatt
     9421 caccgaaata ggtggaacat tgacaggatg cagcagtgca tcagcatcat ttactattac
     9481 tcaatctgct aatttacttc aagtaacagc caacgtaacc cgaaacgaca attgtaatcc
     9541 taataaggga agaattacag caacgggtca gtttggaaca gccccctatc aataccaatt
     9601 ggtacttgcg ggtgatcctg cccctacagt gggaacttgg gttggtagtg ctaccaatgt
     9661 tttcaatgta gaaggaggaa attatgtagt atatattaaa gatgcaaatg gttgtattca
     9721 agcggctcct gtttttgttc ctaccgatcc ggctcctcaa atttcggcta cagttacaaa
     9781 tttatgtact gctacacaag gaagcttctc aatagaagta actttaactt catccggaat
     9841 tgcaccctat acttttagtt tgaatggtgg ggcttatcaa acacaaacgg ttcctacgtt
     9901 tacttataca aatttaaatt caggaaatta taacattcgc gtgagagatt acaatggttg
     9961 tattcataac gttttaattt caatccctgt tcctttaaat ttgactccgg ctgtaacagc
    10021 tcaagtttct tgtgctaaca atgatggaat tataactatt actccatttg gcggaagcgg
    10081 aacctttact tatgaattac aaaacacatc cggaacagtt attgtagcag ctcaatcatc
    10141 tccggttttt aatggtttag cggctggaag ttatattgca attattaaca atacaacaac
    10201 aggttgttcg gctcaagctc caatagttct ggaagcacca actccggtaa cttttacaac
    10261 ttcaataagt gatataagtt gtaacggtgg aaacaacggg agtattatag tttctttacc
    10321 agcaaccaac aacaacccaa tgtatacttt tgtgttaaca gacggtgtaa atactccaat
    10381 ttctcaaaat aatggaaact ttaccggatt agttgcggga acatatacca ttactgtaac
    10441 ttcaggaaga aattgtcaat tacaacaagt tgttgttatc aatcaacctg ctgtattaac
    10501 tgtttcagct tcagcgactg cttttgcttg taatagttca aatgttgtac aaacagcgac
    10561 tattactgct tctgctgcat ttggaacagc tccttatgtt tatagcatta acggaaccaa
    10621 tttcttttca tctaacacat ttacaatagt tgataacgga agcgttcaaa atataaccgt
    10681 aacggttcgt gatgctaaca actgttccgc aacaacttca gtaaatatta atcctttacc
    10741 gacaataann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc taacaactgt
    10861 tccgcaacaa cttcagtaaa tattaatcct ttaccgacaa taaccgcagt tactgcttca
    10921 actgtaactc cgttaacttg taccaataac gggactgtcc aattaaatgt aaccggtgga
    10981 tcaggtgact ttacttttga tttattacct ctaggaagtt atccttctgt gacacccggt
    11041 attggaatat ttaccgttaa cttcacatta acaacacccg gaacatatac tttccaggta
    11101 actgataatg ttacaggttg ttattttatt actgctcctt atgttattgc tcctttcaac
    11161 accattcaag cttcaatcat taattctacg gcggtgactt gttttggtga tacaaatgga
    11221 acaattacaa tgaatgtttc cggatattca ggaaacataa cttatgtagt gaacgacatt
    11281 caaggaaata cggtggcaaa cggtagtgga aacacatcaa ttaatccatt tgttattaca
    11341 ggattatcag gtggaaatta tcaagttatt gtaactgcta cggatagtcc attttgcagt
    11401 gcaagttcta actttacagt tgtttcctct ccaagtctgc cattagtttt agtagcaaac
    11461 caaacagcaa gtgtaacctg tacaaataac caaggagaaa ttgccgctag tgctagcgga
    11521 ggatggggaa cttatcaatt tcaattggta aataatacta cctcaactac tgttcaaccc
    11581 tatgcttcaa attcattatt tacaggctta tctgctggaa attatactat ttccgttcag
    11641 gatgccggag gttgtgttat tagttcaacc attatattgg ttcaaccaag tttaatttct
    11701 gctactttaa cagcaaccaa tactgcttta ctttgctttg gggacactaa tgcttcggta
    11761 acagcaacaa atgttacggg cggtcaaggt gtataccaat attttttaaa tacctataat
    11821 gcttcaggaa ctacaattgt aagcagttca ggtggtcaaa ccagcccaac ttttaacgga
    11881 ttaggagcag gaatttacag catcaccatt acagatggat ggaattgtga ttttacttct
    11941 aatacggtta taattacaca accaactcca gttgttgctt ccttaaactt aacaagctct
    12001 ttaacttgtt taaataatgc tgtaataaca atttcagcaa gtggcggaac agctccctat
    12061 caatatagtt ctgatggagt tacgttttct aacaacacaa catttaatgt tggtcctgga
    12121 acatatcagt attttgtgag agatgctaat aattgtgccg tagttcaaac aaatcaaatc
    12181 acaatcaatg caattcctgc tttagaatta aatttgaatt tgaacagtgc gactatcaac
    12241 tgtttcggcg aaagtactgc aacgataatt gctaatacaa ccggcggatt aggaaattat
    12301 tcttatacgc ttttggatac agcaaataac gtaattgcag gacctcaaaa tagcggaact
    12361 tttgcaaacc ttcctgctgg agattatatc gtaagagtag acagtaatga ctgtaatgct
    12421 gtttcaaatg taattcaaat aacaaatcca tcccaattgt taatctctgc attaacagta
    12481 aataatgtgc tttgtaatgg aggaacaaat ggtagtgtag taattcaaac ttccggagga
    12541 acaggaactg ttcaatatgc tatttcgcct aacttgaatc aattctttaa cacgggaaca
    12601 ttcaataatt tagctcctgg taactatgat attattgttc aagatcaaaa cggctgtttt
    12661 attttattag atgttgatat tattgaaccg gctccacttg ttgctgatgt tgcaaatgta
    12721 gttcaaaacc tttgtttagg tgataatgat ggttcgtttg acatagttat ctcaggtgga
    12781 actgccccat attcaactag tttaaataac caagatccaa gtgcttttgt tgaaggacaa
    12841 ttatccttct caggtttggt gggtggaaca gattatttca tttttgtaaa agatgccaat
    12901 ggctgtgaaa ctattgctta tgtattctta aatgcacctg ttgaagttat cccaactgtt
    12961 gatatcgttt acaattgtac cggaaatatt cctggaaata cagtaactgt ttctgttaat
    13021 tcttctgcag ttggaaatgt tcaatatgca ttagatggag gaacttacca accatcaaac
    13081 ctattccaaa atgttcccac cggaaatcac actatttctg ttcaacacac aaatggatgt
    13141 atcaaaacag ctgactttat tatcaacgca aatcaaccat tggctgtttc gttagctaac
    13201 gttcagaatg ttttgtgtaa tagtggaaat acaggaagcg taacggttgc aactactgga
    13261 ggaacaggaa caattgaatt tgctatttcg ccagatttaa caaacttttc taccaataat
    13321 actttctcaa atttaattgc aggaacttat acaattattg ttcgtgacgg tattggttgt
    13381 agtgtctctc aacaagtaat cataactcaa ccgacacttg ttaccacatc agtttctgcc
    13441 attgctcagg aattatgtac taatgacggc aacggtagca ttacaattgc aatagcgggc
    13501 ggagttgctc cttattcaac gagtttaaat aatagcagtc ctggaagttt tgtgctaaat
    13561 caagtaacat tctcaagctt ggttggtggt caaacttaca caatttatgt tctagatgcc
    13621 aatggttgta tcacctcaat tccggtaacg ttagatgctc cggttacaat taatccaaca
    13681 gttaatgtag cctatgtttg taacggtaat actgttggaa actcagtaac ggtttctgtt
    13741 aatactgctg ttcaaaatga tgttacctat tcattggatg gtggtacttt ccaaacttcc
    13801 gctactttta caaatgtggc tgttggaaat cacaccattg ttgtaagaca taataatggt
    13861 tgtgaaaaga gtgtttcgtt tatcatcaat ccttcaactc caattcaagc ttcttttacc
    13921 tcaacgaatg tgacctgtaa cggtttaaca aatggtttaa ttcaagtagc tgcatctgga
    13981 ggaaatggag cattggaata tgcaatttct cctaatttaa ccagtttcag tgcttcaagt
    14041 actttcaata atttagcagc aggaaactac actgttactg tgagagatgc tcttggttgt
    14101 tcagtcacat tgaatatcac agtaactcaa ccgaatgctc taaatgcatc actagttact
    14161 atgttcccgg atttgtgtta cggtgataat gttggtgcta ttgaaatatc aattgctggc
    14221 ggaacggctc cttattcaac tagcttaaat aacgaaaata actatgttgt taaccaagtt
    14281 ttatttgaga acttatcagg aggtcaatcc tatactattt atgtgagaga cactaaaggt
    14341 tgtacctcta ctttagaagt tgccgtgagt ggaggaataa gtataaatcc gggaattacc
    14401 attacgtatg actgttttgg aagtgctcaa tttaattccg taaaaattaa agtaggacag
    14461 gaaatttcat ctcaggtaac ctattcatta gatggaggtg ctttccaaaa cggaaatgta
    14521 tttacaaatc ttacaaacgg aaatcatacg ataactattc ttcacgctct tggttgttca
    14581 gaaacgactt cctttactat tgagaattat attccaattt cgttgacttt agtagaaagt
    14641 aatctgaatc aaattacagc aacggcaacc ggtggaagcg gaagttatac ttactatttg
    14701 aatggtgtga attatggaga tttgaatgag cttcaaatat tcgcaaacgg aacttatacg
    14761 gttcttgttg aggatagtaa cggatgtact gctgaagcac aaattacgat ggattttatc
    14821 gatatcgata ttccgaactt ctttacgcca aacgatgatg gtgataacga tacgtgggtt
    14881 cctgaaaatt tagaaggttt cccgaatgca aaagttgaag tattcgaccg ttacggtaga
    14941 attattgttc aattcggaac gaaaggatct tgggatggaa catataatgg ctctctttta
    15001 cctaccggag actattggta taccatcacc cttgacggtg gaagacaatt tgttggaaac
    15061 gtaaccttat acagataaaa atttaattta ataaccaact ccaaatagcc acaagtttgg
    15121 agttggttta acctaaaaaa cctacaccca tgaaaaaatt agccccattt ttcattttac
    15181 tcgctttttt aaacggaaaa gcacaagaat tgaacctacc ggcctttact cagtatttgg
    15241 ctgacaatcc atttgtaatt tcaccggtat atgccggtat tggtgataat attaaaataa
    15301 gagccaacgg attgacgcaa tgggttggca ttaaagatgc accaaaaaac atgtccttag
    15361 cagctgatat gcgtattgct gatcgttcag gtgttggttt gtatttatat agcgaccgca
    15421 atggaaatac ccgtcaatcg ggagcaaaag tgagttttgc tcatcactta gttttagata
    15481 aatatgaaga tcaatatttg tcttttggta tttcgtataa tttaaatcaa tttcgaattg
    15541 agattgaaaa ttttaccggt caggatgccg ctgttgtaga cgaccgtcgt ttaaccaacc
    15601 acaatgtaga tgttggtgta ttgtatagaa taaacggatt ctatatcagt gctactgcag
    15661 ccaatatttt agataaaaat atcgatgtat ttaatgacat tgaaccgctt gaattgagaa
    15721 actaccaatt atttactggg tataaatggc gaagtaatac caatagtgat tttgaagtag
    15781 aaccatcagc tttctttcaa tattttgaaa gtgataaaag atcaacaacc gatttgaatt
    15841 taaagatgcg tttttataac tttgatgatt attattgggc ggggatttct tatcgttttt
    15901 tgaatgatca attattggag cctttaaaca ttggaccaat gttaggttta aaaagaagta
    15961 atttttattt tgcttattcc tatcaggtaa cgctgaacaa attgattaat tataattcag
    16021 gaacgcacat gattaccatt ggactagatg ttttccaagg aatcagcaat tgtccttgta
    16081 cctattaaaa acataaaatt atgggcacta ttcggttaca aaacattcgt acattttctt
    16141 ttcatggttg tttgcttgag gaaagtaaaa ttggctctga ttattcaatt gatttagaag
    16201 taaaaaccga tatgcgaaag tcagctttat cagatgattt acatgacacg gttgattatg
    16261 ttttattaaa ccgaattgtg gtggaagaaa tggccattcg ttcgaaatta ttggaacatg
    16321 tagcccatcg aattatttct agaattttta aagagattcc ttctatagct cgggttttga
    16381 ttggggtatc aaaactcaat ccacccattg gtggtgatgt ggaagcagtt accatccaaa
    16441 tggaagaata tagaagttaa ttttggttag cttagtcagt cgcactttga tcttgtgtca
    16501 agtttcagtt tattaatata aaagtttttt gactttaaac tttttaactt taaacttttt
    16561 atttacattt gccatccata catcaggcgt tgtggccgag cggctaggca ccggtctgca
    16621 aaaccgtcta ccccggttcg aatccgggcg atgcctctga aaccttcaag gaaacttgga
    16681 ggttttttta tgctaaattt ttagaaaaaa ttaattaaag ataatgcaaa accgtct
//
LOCUS       DAOTCW010000046        16360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2009_length_16360_cov_46.312420, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000046 DAOTCW010000000
VERSION     DAOTCW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 16360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16360
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2009_length_16360_cov_46.312420"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="P5335_05460"
     CDS             complement(<1..302)
                     /locus_tag="P5335_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRZ74359.1"
                     /translation="MPNDNLKSYLHLHLIVFIWGFTAILGELITLDALPLVWFRMLLA
                     VGFIILFMAYKKTNFVVPRKTIFGFLLLGLVIALHWLTFFKAIKVSNVSVTLACLST"
     gene            complement(289..1368)
                     /locus_tag="P5335_05465"
     CDS             complement(289..1368)
                     /locus_tag="P5335_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRZ74360.1"
                     /translation="MLKIIDKYILKRYLATFVVMLLLFIPIGIVIDVAEKINKILESK
                     VPFKAVAIYYLDFTIYFANLLFPIFLFLSVIWFTSKLANNTEIIAILSSGISFTRFLR
                     PYILGATMVSVFALIMGFFLVPKASKGFNDFRYTYLKNNAEIRENSNVFRQISDDEFI
                     YVSNFNSVSKTAFNFTLEKFKGEKLEYKILASRLKWNEKDSTYTMFSYQKRKVGIYGD
                     IIEKAEKKDTIFNFDLEDLTPVIYIAETLTLPELNKFIKKEKARGNANINTYLVVLYR
                     KYSLPVTAFILTIIAVSVSSMKRRGGMGVNLAIGIVLAFTYIFFDKIFGTMAEKSSIP
                     PFIAVWFPNFVFGILAVYLLKNAKR"
     gene            complement(1384..2514)
                     /gene="tgt"
                     /locus_tag="P5335_05470"
     CDS             complement(1384..2514)
                     /gene="tgt"
                     /locus_tag="P5335_05470"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRZ74361.1"
                     /translation="MKFDLLTTDPNSKARAGSITTDHGVIETPIFMPVGTVASVKGVH
                     QRELKEDINPDIILGNTYHLYLRPQTEILEKAGGLHKFMNWDRNILTDSGGYQVYSLS
                     SNRKIKEEGVKFKSHIDGSYHFFSPENVMEIQRTIGADIIMAFDECTPYPCDYRYAKR
                     SMHMTHRWLDRCINHLENLPFKYGYEQTFFPIVQGSTYKDLRQESAEYIANAGAQGNA
                     IGGLSVGEPAEEMYAMTEIVTAILPQDKPRYLMGVGTPVNILENIALGIDMFDCVMPT
                     RNARNGMLFTAHGSINIKNKKWEDDFSPIDEMGITFVDTEYTKAYLRHLFAANEYLGK
                     QIATIHNLGFYMWLVREARKHILAGDFRVWKEQMVKQMSQRL"
     gene            2707..3555
                     /locus_tag="P5335_05475"
     CDS             2707..3555
                     /locus_tag="P5335_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRZ74362.1"
                     /translation="MKPNTQQLNDFATQVRRDILRMVHAVNSGHPGGSLGCTEFFTAL
                     YQVILDRKEGFNMDGNGEDLFFLSNGHISPVFYSVLARSGYFPVSELATFRLLNTRLQ
                     GHPTTHEGLPGIRVASGSLGQGMSVAIGAALAKKLNGDKHLIYSLHGDGELQEGQNWE
                     AMMYAAAYKVDNYIATVDLNGKQIDGATDEVLPMGDIKAKFIAFGWDVLEITEGNNIE
                     AILAGMSEAKSRTGKGKPVCVLLHTEMGNGVDFMMHTHAWHGKAPNDDQLAKALEQNP
                     VTLGDY"
     gene            3624..4577
                     /locus_tag="P5335_05480"
     CDS             3624..4577
                     /locus_tag="P5335_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRZ74363.1"
                     /translation="MKKYTNTGSKDTRSGFGAGMTELGQKKENVVALCADLIGSLKFD
                     DFKKNHPERFFQIGIAEANMIGIAAGLTIGGKIPFTGTFANFSTGRVYDQIRQSVAYS
                     DKNVKICASHAGLTLGEDGATHQILEDIGLMKMLPGMTVINTCDYNQTKAATLAIADH
                     HGPVYLRFGRPVVPNFMPADEPFVVGKAILLNEGSDVTIIATGHLVWEALIAAEKLEE
                     KGISAEVINIHTIKPLDEEAILKSIAKTSCVVTAEEHNILGGLGESVARTLSLNNPVP
                     QEFVAVNDSFGESGTPDQLMEKYQLNHQAIIAKAEKVINRK"
     gene            complement(4630..5598)
                     /locus_tag="P5335_05485"
     CDS             complement(4630..5598)
                     /locus_tag="P5335_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidylprolyl isomerase"
                     /protein_id="HRZ74364.1"
                     /translation="MLLKSLKVPILMVLISFLSFSCKDDDDATTVTPPNDRTEQYASD
                     IDSIEKYLKTNYMTLDSDLNAVITKIPTGGSQVSIWDQTDYPLNSITVKNDTRLTIRT
                     DGGIEDPVEYKLYYILLNEGGGQNPLTIDSSFVDYRGWNLDNVEFDRTSTPVWTSYPI
                     TNVFISGFRQILPIMKTAESIVENPDGTINYINAGNCLVFIPSGLAYYNSPQGANLPA
                     YSSIVFQINLKTLDKIDHDSDRILSYMELYGTETDYFMQDSDGDLIPDFLDTDDDGDG
                     FLTRNEFKISGTDPQEYYDFTTIPDCSGNTINPIRLKRYLDPSCHQ"
     gene            5726..6106
                     /locus_tag="P5335_05490"
     CDS             5726..6106
                     /locus_tag="P5335_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRZ74365.1"
                     /translation="MRVDKYLWCVRYFKTRNMVTELCKKNHVTVNGIVAKPSREVFPS
                     DAVTFRRDQITYKIRVLAIPENRVGAKLVDIYRKDETPEEAFAHLELLKLSKEHYRKN
                     GEGRPTKKDRRDIENYTEDTNETE"
     gene            6093..6671
                     /locus_tag="P5335_05495"
                     /pseudo
     CDS             6093..6671
                     /locus_tag="P5335_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165667.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            6680..7180
                     /locus_tag="P5335_05500"
     CDS             6680..7180
                     /locus_tag="P5335_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HRZ74366.1"
                     /translation="MPQNIILTHQEIEHKIKRIAYQIYETFVDEKEIVIAGIAKNGFI
                     FAQKLAEDLEKIAEFKVTLCEVHANKHNPFEPITTSLDKATYENKGLILVDDVLNSGT
                     TLVYGVKHFLDVPLTKFKTAVLVDRNHKKYPVKVDFKGISLSTSFQEHVQIVFEEGNS
                     YAYLSQ"
     gene            complement(7169..7687)
                     /locus_tag="P5335_05505"
     CDS             complement(7169..7687)
                     /locus_tag="P5335_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRZ74367.1"
                     /translation="MKKIILLGYMGSGKTTIASKVADVLKKKHLDLDKLIEKKEGKTI
                     NSIFATKGEIYFRKIEHEVFKDLMESQDDFVLSFGGGTPCYANNHLFLKEKEVISIYL
                     KTSVEELSKRLLQSKAERPLIAQMNEDELKEFIAKHLFDRSYYYHQAQHFISTDNKLE
                     KEVVQEIIKLLT"
     gene            7816..7888
                     /locus_tag="P5335_05510"
     tRNA            7816..7888
                     /locus_tag="P5335_05510"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7849..7851,aa:Lys,seq:ttt)
     gene            7918..7990
                     /locus_tag="P5335_05515"
     tRNA            7918..7990
                     /locus_tag="P5335_05515"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7951..7953,aa:Lys,seq:ttt)
     gene            8037..8118
                     /locus_tag="P5335_05520"
     tRNA            8037..8118
                     /locus_tag="P5335_05520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8071..8073,aa:Leu,seq:gag)
     gene            8372..9268
                     /locus_tag="P5335_05525"
     CDS             8372..9268
                     /locus_tag="P5335_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRZ74368.1"
                     /translation="MNNVKNVFSIKDLENLSGIKAHTIRIWEKRYNVLEPMRTETNIR
                     YYDLVALQKLLNITLLHNHGYKISKISKLSPDKINVMVREIVNEKSVKHHAINAFKMA
                     MMNFDQVLFFNTYSNLLSEKSFREVFYEVFIPLMNEIGLLWQAETITPAHEHFISYLI
                     KQKLLVNTEKIQLLEPTRTDKVFVLFLPANEIHELGLMYLNYEVILNGYKTIYLGESV
                     PIDSLKDIKRYFDNVVYISYMTVEPTKDNISDYMKEIHKEILEGENAELWAVGRMTEF
                     IKPNQNSKIKTFNSILELSKEL"
     gene            9323..10783
                     /gene="crtI"
                     /locus_tag="P5335_05530"
     CDS             9323..10783
                     /gene="crtI"
                     /locus_tag="P5335_05530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013185952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HRZ74369.1"
                     /translation="MKKNIHIIGSGFSALAAACYLAKAGHDVTVFEKNSTIGGRARQL
                     KKEGFTFDIGPTWYWMPDVFERFFADFGKKPSDYYKLIKLSPAYQVYFGIRDFVTIAD
                     NLPEIINTFESIEKGSGRTLEKFMAEAKKNYDIAIKDLVYNPGVSPLELITLETAKKV
                     GQFFSTISKEVRKRFKNDKLIQVLEFPVLFLGAKPSDTPAFYSFMNFADFGLGTWHPK
                     NGMYSVVLAMETLAKELGVVVETNASVDKIIVENKVAIGIQVNGKKHFCDIVLSGADY
                     HHTETLLDKKDRVYSESFWEKKTFAPSSLLFYIGFDKKIENVEHHTLFFDVSFEKHAE
                     AIYDNPKWPEEPLFYASFPSKTDSSVAPSGKEAGIFLIPLAPGIEDTEAIRKKYFEKI
                     ITRFEKLTQQNIKNNILFMESFCVNDFIKDYNSFKGNAYGMANTLMQTAFLRPKLKSK
                     KVENLYFTGQLTVPGPGVPPSLISGKLVSELIEKYS"
     gene            10805..11644
                     /locus_tag="P5335_05535"
     CDS             10805..11644
                     /locus_tag="P5335_05535"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRZ74370.1"
                     /translation="MKSIFDHVSFECSKLVTKSYSTSFSSAVKMLAPSIREDIYNIYG
                     FVRFADEIVDSFHQYDKELLFELFEHDLEKALQNKISLNPILNSFQHTVNRYEIPKDL
                     IDAFMKSMKQDLYKKEYQTTAEYQEYIYGSADVVGLMCLKVFVKGNEELYEELKHSAM
                     RLGSAFQKVNFLRDLKADFEDLNRTYFPNTNLNQLDETSKQEIIAEIESDFAAGFVGI
                     QKLPIEAKFGVYTAYIYYKKLLSKLKKTPSLEIKNTRIRVPDYEKAGLLAKCYLNYRL
                     NII"
     gene            11657..12097
                     /locus_tag="P5335_05540"
     CDS             11657..12097
                     /locus_tag="P5335_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRZ74371.1"
                     /translation="MWIHILIFFATFFMMEFMAWFTHKYIMHGILWHLHADHHKKDHD
                     SWFERNDTFFIFYAIVSISFFLLWKYEILELGLAIGLGILAYGLTYFFVHDIFIHQRF
                     KLFRNANNIYARALRRAHKMHHKHIGKEHGECFGMLIIPFKYFK"
     gene            12226..14145
                     /locus_tag="P5335_05545"
     CDS             12226..14145
                     /locus_tag="P5335_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRZ74372.1"
                     /translation="MNIKVTLLLCLFISFSVFGQKNIDPTPADIDLAKSIRSKYEKSD
                     VAILESMENITFGYNKSSSNVTVDLSVDELLMNINHRADIFKYEFYDSSSKIENFTIK
                     YRNDKLAYFAVQDEFYKDKDLFYNDARVKHVKIDFPVQGYTYNYHIDKKFEDVKYFTS
                     IYFNDEFPVIKKKIKVTIPKWLNLELKEMNFQGHSIVKTQTTDAKKNTTTIVYEFNDI
                     PALISEENSPGRSYLYPHLLVIAKSYEMNGEKITLFNSTADLYKWYKSLIDQMKDDPE
                     QLKSKVTELTAKAKTDEEKIKNIYYWVQDNIRYIAFEDGIAGFKPDDSQNVFKKRYGD
                     CKGMANLIKQMMIIAGFDARLTWIGTKHIAYDYSIPSLSVDNHMICTVLYKGKQIYLD
                     GTEKNISLEEYAERIQNKETLIENGDTYIIGKIPTVQASQNKETFRAKLVVDGEKLKG
                     SCKRTYLGESRTEFQNIYNSFETDKKAEALERYLSKNDKNNHVTSIKTSDLKSREQAL
                     TIDYELESENRLSNFDGEMYLNFEFMNEYKKFTLKDRKTDYELDYKTLYDSEITIVLP
                     AGYKVEKLPENLIQKEEDYQVEVSFSQKGNELLYRKVFIFNNAILPKNKQEKWNTTHK
                     KLVEFYNSSITLIKS"
     gene            14150..16297
                     /locus_tag="P5335_05550"
     CDS             14150..16297
                     /locus_tag="P5335_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HRZ74373.1"
                     /translation="MKKIIILCLLIATTINAQDNVKIRDFFWGKQDTHSKTVAIPDKW
                     KNESAVIIYKFDFYDYHKFGKNVNYTSAKRIRVKLLDEAAVKEFSEFTFTERFLSSKG
                     YAMKRGTNSIGIKVIKPDGKENILDVSKESVVADKEKKIAIPNLEIGDIIDYYLHSEE
                     PFKSIIEVGFDPVETNLGDVYPIMEYKLTFQTENDFFVNFNTYNGAPKLTQIPTKNSG
                     ERIYELAATNLDKNDFPRWFYPLVELPCYKFQVFFARSGKFEERAKAFLSEKEKDVKS
                     TVTKEDIFNYYDSKFLAEGNISQINDFLKGKKFKDDEEKIREVYYYTRHQYFTQYVEA
                     FVAKEANIFYPFELYPNAIFFNTEEHFIRHFMFFLKKNDISYDIVVGTDRYNGDIKDL
                     LIQQNVSILLRVNTPKPIFLEFFTPFTSADQFDYNLENTKAYLLQVSKGKKIIDAESI
                     TLPSSTKNDNINRSVTKISLKEDLSSFNVNREQSLFGHYKEGEQSDKLYFFDYVYEDY
                     KKYGNTPLMELVKNKKQRAQYTKEFDALIAKSKENQKESFIKSVKEEFEIDIENYSME
                     IKNTGRFGRNEPMQYTEKFTITDQYIKKAGNNLMVELGKFLTSQIELSKKEKERTNNV
                     YMTFPRQIEQEIQFEIPAGYTVSGLEKFNKKVENETGGFVSTATQNGNLITIKTTKYY
                     SNYFEPNSNWKKMVDFLDASYQFTQEKVLLKKN"
BASE COUNT         5879 a         2601 c         2657 g         5223 t
ORIGIN      
        1 gttgataaac acgctaaagt gacagaaaca ttagagactt taatggcttt aaaaaaagtg
       61 agccaatgta aagcaatgac caatcctaga agtaaaaacc caaaaattgt ttttcttgga
      121 acaacaaaat ttgtcttttt ataggccatg aacaaaatta taaaaccaac tgccaacaac
      181 attctaaacc ataccaaggg taaagcatcc aaggtgatta attcccccag aatggctgta
      241 aatccccaaa taaaaacaat taaatggaga tgtaaataac ttttaaggtt atcgtttggc
      301 atttttcagt aaataaacgg ctaatatacc aaaaacaaaa tttggaaacc aaacagctat
      361 aaaaggtgga atactcgact tttcagccat ggtgccaaaa atcttatcga agaaaatata
      421 agtaaaagca agaactattc cgatggctaa gttcactccc attccgccac ggcgtttcat
      481 ggaagaaaca gaaactgcaa ttatagttaa aataaaagca gtaaccggca aactgtattt
      541 gcgatacaac actaccaaat aggtatttat attagcgttg cctcgcgctt tttctttttt
      601 aataaattta tttaactccg gcaaagtaag cgtttcagct atatatataa caggcgttaa
      661 atcttctaaa tcaaaattaa aaatggtgtc ttttttctct gctttttcaa taatgtcacc
      721 ataaatacca acttttcttt tttgataact aaacatggta tatgtgctgt ctttttcatt
      781 ccatttcaat ctgctggcta aaattttgta ctccaacttt tcccctttaa atttttctaa
      841 agtaaaatta aaggcagttt ttgaaaccga attaaaatta ctgacataga taaactcatc
      901 atcactaatt tgcctgaaaa catttgaatt ttctctaatt tcagcattgt tttttaaata
      961 agtatacctg aaatcattaa accctttact ggcttttggt acaagaaaaa agcccatgat
     1021 taaagcaaaa actgaaacca ttgttgcacc caatatatac ggtcgtaaaa atcgggtaaa
     1081 tgaaattcct gaacttaaaa tagctataat ttccgtatta tttgccaatt tggaagtaaa
     1141 ccatatgaca gataaaaata aaaaaatagg aaataataga tttgcaaaat aaattgtgaa
     1201 atcgagataa tagattgcta ccgctttaaa aggcactttg ctctctaaaa ttttattgat
     1261 tttttcagct acatcgatta caatcccaat aggaataaaa agcaataaca tcaccacgaa
     1321 agtagctaag tatcttttta atatgtattt atcgatgatt tttaacatcc tatttccagt
     1381 taattataac ctttgactca tttgttttac catttgttct ttccaaaccc tgaaatcacc
     1441 tgctaagata tgttttctcg cttcacgaac cagccacata taaaaaccga gattatgaat
     1501 ggtcgcaatt tgtttcccaa gatactcatt tgctgcaaat aaatgccgta aataagcttt
     1561 ggtatattcg gtatcaacaa aggtaattcc catttcatca ataggcgaaa aatcatcctc
     1621 ccacttttta tttttgatat ttattgatcc atgagctgta aacaacattc cgtttcgggc
     1681 atttcgggtt ggcatcacac aatcgaacat atcaattcct aaggctatat tttctaatat
     1741 attgacaggt gttcctactc ccattaaata acggggttta tcttgcggta aaatagcggt
     1801 tacgatttca gtcatcgcat acatttcctc agcaggttca ccaactgaga gtccgccaat
     1861 agcgtttcct tgggcccctg cattggcaat atattccgct gattcctgtc ttaaatcttt
     1921 atacgtactc ccttgaacaa taggaaaaaa agtttgttca taaccatatt taaaaggaag
     1981 attttccaaa tgattaatac atctgtctaa ccaacggtgt gtcatgtgca tggaacgttt
     2041 ggcatagcga taatcacatg gataaggtgt gcattcatca aaagccatga taatatcagc
     2101 tccaatagtt cgttgaattt ccatcacatt ttccggagaa aagaaatgat aggaaccatc
     2161 aatatggctt ttgaatttca cgccttcttc cttaattttt ctgttggaag aaagtgaata
     2221 aacttgataa ccaccactat cagtcaagat atttctatcc caattcatga atttgtgtaa
     2281 acccccagct ttttctaaaa tttctgtctg tggacgcagg tataaatgat aagtatttcc
     2341 aagtataata tccggattga tgtcttcttt taattcacgt tgatgcactc ctttaacaga
     2401 agcaaccgtt ccaaccggca taaaaattgg ggtttctatt acaccgtgat cagttgttat
     2461 gcttcccgca cgggctttcg aattagggtc agtcgtcaat aaatcaaact tcatatatgt
     2521 tttttacagt gggcaaagat aaaagatttc gcttttctgt tgagtgtttt gggaattatt
     2581 tatgtttcta attaacgctg tttactaaca atttatcttg aaaactttag caaaattcta
     2641 attccctctt gaaacaaaat ctaatatttg atttaccttt gccgaagttt aacatttatc
     2701 attaaaatga aacctaacac acaacaacta aacgactttg caacacaagt cagaagagac
     2761 attcttcgaa tggtacacgc cgtaaattcg ggacatcccg gaggttcttt aggatgtaca
     2821 gaatttttta cagccttata ccaagtcatt ctcgacagaa aagaaggatt taacatggat
     2881 ggaaatggag aagatttatt tttcttatcc aatggccata tttcacctgt attttacagt
     2941 gttttggccc gaagtggtta ttttccggta tcagaattgg ctacttttag attattaaat
     3001 acacgtttac aaggacatcc aaccactcat gagggtttac ccggtattcg tgtagcttcc
     3061 ggctcattag gtcaaggcat gtccgttgct attggtgctg ccttagcaaa aaaattaaat
     3121 ggtgacaaac acttgattta ttcacttcac ggtgatggcg agttacaaga aggccaaaat
     3181 tgggaggcta tgatgtatgc agcagcctat aaagttgata attatattgc aacggttgat
     3241 ttaaatggta agcaaataga tggagcaacg gatgaagttt tacccatggg tgatattaag
     3301 gcaaaattta ttgcttttgg ttgggatgta ttagaaataa ccgaaggaaa taacattgaa
     3361 gctattttag ccggaatgtc tgaagctaaa tcgagaaccg gaaaaggaaa accggtttgc
     3421 gttttgttgc atacggaaat gggaaatggt gttgacttta tgatgcatac gcatgcatgg
     3481 catggaaaag ctcccaacga cgatcaatta gcaaaagcgt tagaacaaaa ccccgtaact
     3541 ttaggtgatt attaaattgc tttttgcact atgctctaag ccttatgctt attgcctatt
     3601 gcctaaagcc catagcaaaa aaaatgaaaa aatataccaa tacaggaagt aaagatactc
     3661 gttcaggatt tggagcagga atgaccgaac tcggacaaaa aaaagaaaat gtggttgcct
     3721 tgtgtgccga cttaattggc tcattaaaat ttgatgattt caaaaaaaat catccggaac
     3781 gttttttcca aatcggaatt gcagaagcca atatgattgg aattgcagcc ggattaacca
     3841 ttggtggtaa aattccgttt acaggaacat tcgccaactt ttctaccgga agagtgtacg
     3901 atcaaattcg tcaatcggtt gcttattcag acaaaaatgt aaaaatttgt gcttctcatg
     3961 ccggattaac tttaggagaa gacggagcaa cacaccaaat tttagaagac atcggtttga
     4021 tgaaaatgct tcccggaatg acggtaatta acacttgtga ttacaatcaa actaaagctg
     4081 cgaccttggc cattgccgat caccacggtc cggtttattt acgtttcggt cgtccggtag
     4141 tgccaaattt catgcctgct gacgaaccat ttgtagtagg aaaagcaatc cttttgaatg
     4201 aaggaagcga tgtgaccatt atagctaccg gacatttagt ttgggaagct ttaattgcag
     4261 cagaaaaact agaagaaaaa ggaatttctg cagaagttat caatattcac actattaaac
     4321 cattggacga agaagcaatt ttgaaatcga ttgctaaaac aagttgcgta gttactgcag
     4381 aagagcataa tattttagga ggtttaggag agagtgttgc ccgaacttta tctttaaata
     4441 atcctgtgcc tcaagaattt gtagcagtaa atgacagttt tggtgaaagt ggcactccag
     4501 accaattgat ggaaaaatac caattaaatc accaagctat tattgcaaaa gcagaaaaag
     4561 taatcaatag aaaataacca tcctttttac ctcaaataaa aaatcccgat tgctcgggat
     4621 tttttttatt tattgatgac agctagggtc aaggtatctt tttaacctaa ttggattaat
     4681 tgtatttcca ctacaatccg gaatagttgt aaaatcataa tactcttgtg ggtcagttcc
     4741 tgaaattttg aattcatttc tggttaaaaa accatcccca tcatcatcgg tatctaaaaa
     4801 atcaggaatt aaatcaccgt ccgaatcttg catgaagtaa tccgtttccg ttccatataa
     4861 ttccatataa gataaaattc tatccgaatc atggtcaatt ttatctaacg ttttcaggtt
     4921 gatttgaaat acaatactag aatacgccgg taaattggct ccttgtggac tattataata
     4981 agccaatcct gaaggaataa aaaccagaca atttccggca ttaatataat taattgttcc
     5041 atcaggattt tcaacaatac tttctgctgt tttcataatc ggcaaaattt gtctgaaacc
     5101 ggatataaaa acatttgtta ttggataaga tgtccaaaca ggagttgaag ttctatcaaa
     5161 ctcaacatta tctaaattcc aacctcgata atctacaaat gaagaatcaa ttgttagcgg
     5221 attttgacct ccaccctcat tcaataatat ataatacaat ttatattcca caggatcttc
     5281 aattccacca tcagttctaa ttgttaatcg agtatcattt ttaaccgtaa tactattcaa
     5341 aggataatcc gtttgatccc aaatagatac ttgactacct cctgtcggaa ttttagtgat
     5401 tacagcattc aaatcactat ctaaagtcat gtaatttgtt tttaaatact tttcaattga
     5461 atcgatatct gaggcatatt gctcagttct gtcattcgga ggtgttacag ttgtagcgtc
     5521 gtcgtcatct ttgcacgaaa aagataaaaa tgaaatcagc accatcaata ttgggacttt
     5581 taaacttttt aacaacatat ttatttaatt ttttgttctt tttattagct ttattttagt
     5641 tgaatacttt ttttaaaata agtgctgcaa gatacaattt tgatttattt ttgtataaaa
     5701 attaacgtag atttctgaat aaagtatgag agtcgataaa tatttatggt gcgttcgtta
     5761 tttcaaaacc agaaacatgg ttactgagct ttgtaaaaaa aaccatgtga ccgtaaatgg
     5821 tatagttgcg aaaccctctc gtgaagtatt tccatccgat gctgtgacat ttagaagaga
     5881 ccaaatcact tataaaataa gggtattagc aatacctgaa aacagagttg gagccaaatt
     5941 ggttgacatc tatcgaaaag acgaaactcc ggaagaagct tttgcacatt tagaactact
     6001 aaaattatcc aaggaacatt atcgaaaaaa cggtgaaggg agaccaacaa aaaaagaccg
     6061 tcgggatata gaaaattata cagaagacac taatgaaact gaataaagat tattgggaaa
     6121 acaagtatca tttgaatgag attggatggg atatcggaac agtttctaca ccgttaaagg
     6181 aatatatcga tcaaattgaa aataaagagt taaaaatatt aattccggga gcaggaaatg
     6241 gatatgaatt tgaatattta tataatgccg gatttcaaaa tgtttttgtg atagatatcg
     6301 ctaaaactcc tttagagaat attcaaacga gaatgcctca ccttgacgat aaacacctca
     6361 ttcataaaga tttttttgag ctaagtgata cttttgattt aattatcgag caaacttttt
     6421 tttgtgcgat tgcacctgat ttacgaaaaa aatatgtgtc aaaaatggac tctttattaa
     6481 attcaggcag taaaatagtt ggcttacttt ttcaatttcc actaactgaa aaaggaccac
     6541 cttttggtgg aaatgaaaat gaataccgag ttttatttga aaatcaattc aatataaaaa
     6601 cattagaaac tgctcataat tcaataaaac caagagaaaa caacgaactc ttttttatct
     6661 ttgagaaaaa atataactta tgccacaaaa tattatactc actcatcagg aaattgaaca
     6721 taaaatcaag cggattgcct atcaaatcta tgaaactttt gtagatgaaa aagaaattgt
     6781 aattgctggc attgctaaaa atggatttat ttttgctcaa aagttagctg aggatttaga
     6841 aaaaatagca gaattcaaag tgacattgtg tgaagtgcat gcaaataaac ataatccttt
     6901 tgaacctatt accacttcct tagataaagc aacctatgaa aacaaaggac tgattttagt
     6961 ggatgatgtt cttaattccg ggactacttt agtttatggt gtgaaacatt ttttagatgt
     7021 tccgttaaca aaattcaaaa cagctgtttt agtagatcgg aatcataaaa agtatcctgt
     7081 aaaagtagat tttaaaggaa tttcattatc cacttcattt caggaacatg ttcaaattgt
     7141 tttcgaagaa ggaaatagtt atgcttattt aagtcagtaa tttaatgatc tcctgaacca
     7201 cttccttttc caatttatta tctgtagaaa tgaaatgctg agcttggtga taatagtaac
     7261 ttctgtcaaa aaggtgttta gctataaact cttttagttc atcctcattc atttgagcaa
     7321 tcaaaggtct ttctgcttta ctttgcaata acctttttga taactcctca acagatgttt
     7381 taagataaat tgaaatcacc tctttttctt ttagaaataa gtgattattt gcataacagg
     7441 gagtcccgcc tccaaaactc aacacaaagt catcttgcga ttccattaaa tctttaaaaa
     7501 cttcatgttc aatttttcta aaataaattt cacccttcgt tgcaaaaatt gaattgatag
     7561 tttttccttc ctttttttct ataagtttat ccaaatccag gtgttttttt ttcaaaacat
     7621 cagctacttt agaagcgatt gtggtcttac ctgaccccat atatccgagt aaaataattt
     7681 ttttcattat aataattcct tgtacagtaa ggggttaaga aagaatctgc caaaaagaca
     7741 aatttaagac aaaattgctt tgaaaaataa ataataagga cgtatatttg cacccgcatt
     7801 caagaaattg catttgactc gatagctcag ttggtagagc acatcacttt taatgatggg
     7861 gtcctgggtt cgagccccag tcgggtcaca atttttttga aattttttga tgctaatgac
     7921 tcgatagctc agttggtaga gcacatcact tttaatgatg gggtcctggg ttcgagcccc
     7981 agtcgggtca caagtaaaga cgttgcgtgc aacgtcttta ctttttttta atattaggcc
     8041 acgtggagaa attggtagac tcgccatctt gagggggtgg tgctgcaagg cgtatgggtt
     8101 cgaatcccat cgtggtcacg gatgaatact ttaaataaaa agacgccaag tttacttgtg
     8161 cgtttttttg tttaaagtat tctcaaagcg gagctttagg gagagacgaa gtcaatccca
     8221 tcgtggtcaa ggatgaatac tttaaataaa aagacgccaa gtttaattgt gcgttttttt
     8281 tgttttaaaa taccatttcc aaaaaaaata tttaaaaacc tttaaagttt gttttatttg
     8341 tttacttttg tttaacaaac aatacgaaac aatgaacaat gtaaaaaatg tttttagcat
     8401 caaagatttg gaaaaccttt ccggcatcaa agcacacact attagaatct gggaaaaaag
     8461 gtataatgta ttagaaccga tgcgtacaga aactaacatt cgttattatg atttagttgc
     8521 ccttcaaaag ctattaaaca ttaccttatt acataatcac ggttataaaa tttctaagat
     8581 ttcaaaactc tctcctgata aaattaacgt aatggtaagg gaaattgtga atgaaaagag
     8641 cgttaaacat catgctatta atgcctttaa aatggcaatg atgaattttg atcaagtatt
     8701 atttttcaat acttattcaa atttactttc agaaaaatcg tttcgtgaag tcttttatga
     8761 agttttcata ccactcatga atgaaattgg attgttgtgg caagcagaaa caatcactcc
     8821 agctcatgaa cattttataa gttacctcat caagcaaaag ctattagtta ataccgaaaa
     8881 aatacagctg ttagaaccta ctcgaactga taaagttttt gtgctctttt taccggcaaa
     8941 cgaaattcac gaattagggt taatgtatct caattatgaa gttatattaa atggctacaa
     9001 aacaatttat ttaggagaaa gtgttcccat tgatagttta aaagatataa aacggtattt
     9061 tgacaacgta gtctacattt cctacatgac agttgaacct acgaaagaca atatatcaga
     9121 ttatatgaaa gaaattcata aagagattct tgaaggagaa aatgctgaat tatgggcagt
     9181 tggtcgcatg acagaattca ttaaaccaaa tcaaaattct aaaattaaaa ctttcaattc
     9241 aatactagaa ttatccaaag aattataatt ttttcataat tttctatttt gtttaacttt
     9301 tttactatat ttgttaaaca ttatgaagaa aaatatacac attattggtt ccggattttc
     9361 tgcattagca gcagcttgct atttagcgaa agcagggcat gacgtaacgg tttttgaaaa
     9421 aaattcaaca attggtggcc gtgctcgaca attaaaaaaa gaagggttca cctttgacat
     9481 tggcccaact tggtattgga tgcctgatgt ttttgaaaga ttttttgctg attttggcaa
     9541 aaaaccttct gattattaca aattaataaa actctctccc gcttatcaag tttattttgg
     9601 gattcgtgat tttgttacaa tagctgataa tttacctgaa ataatcaata cctttgaatc
     9661 aattgaaaaa ggaagtggca gaacactaga aaaatttatg gcagaagcca aaaaaaatta
     9721 tgatattgcc attaaggatt tagtttataa tccgggcgtt tcaccgctgg aattaataac
     9781 attagaaact gctaaaaaag taggtcaatt tttttctact atcagtaaag aggttcgaaa
     9841 acgtttcaaa aatgataaac tcatccaagt tttagaattt cctgtcttat ttctgggagc
     9901 aaaaccaagt gatactcctg ctttttatag ttttatgaat tttgcagatt ttggattagg
     9961 aacatggcat ccaaaaaatg gaatgtatag tgttgtactt gctatggaaa ctttagcaaa
    10021 agaactcggc gtggtggttg aaacaaatgc atcggtagat aaaattatag ttgaaaataa
    10081 agttgctatt ggtatccaag tgaatggtaa aaaacatttt tgtgacatcg ttttaagtgg
    10141 tgctgattat caccatacag aaacattatt ggacaaaaaa gatagagtat attctgaatc
    10201 attttgggaa aagaaaacat ttgctccctc ctcactttta ttttacattg gatttgataa
    10261 aaaaattgaa aatgtagaac accatacgtt attttttgat gtctcctttg aaaaacatgc
    10321 ggaagctatt tatgacaacc caaaatggcc ggaagaacct ttgttttatg ccagtttccc
    10381 atcaaaaaca gattcgtccg tagcaccatc agggaaagaa gccggtatat ttttaatacc
    10441 attagcaccc ggaattgaag acactgaagc aatcagaaaa aaatattttg aaaaaataat
    10501 tacacgtttt gaaaaattaa cacaacaaaa tataaaaaat aatattcttt ttatggaatc
    10561 attttgtgta aacgatttca taaaagatta caattccttc aaagggaatg catatggaat
    10621 ggcaaataca cttatgcaaa cggctttttt gagaccaaaa ttaaaaagta aaaaggtgga
    10681 aaatctctat tttactggtc aattaacagt gcctgggcct ggtgttcctc cctctttaat
    10741 ttcgggaaaa ttagtttcag aattaattga gaagtattca taaatcaagt tataagttac
    10801 cgctatgaaa tcaatttttg accatgtatc ctttgaatgc agtaagttag tgactaaatc
    10861 ttacagcaca tccttttctt ctgccgtaaa aatgctggct cccagtattc gggaagatat
    10921 ttataatatt tatggatttg ttcgatttgc agatgaaatt gtagattcgt ttcatcaata
    10981 tgataaagaa ctcctttttg aattatttga acatgattta gaaaaagcat tacaaaataa
    11041 aattagttta aatccaattt taaactcttt tcaacatact gtgaatcgtt atgaaatccc
    11101 aaaagattta attgatgcgt ttatgaaaag tatgaaacag gatttataca aaaaagaata
    11161 tcaaaccact gctgaatacc aagaatatat ttatggttct gcagatgtgg ttggcttaat
    11221 gtgtttaaaa gtttttgtaa aaggaaatga agaattatat gaagaattaa agcacagtgc
    11281 catgcgattg ggttctgctt ttcaaaaagt gaatttttta agagatttga aggctgattt
    11341 tgaagattta aacagaactt attttcctaa tacgaattta aatcaactgg atgaaacttc
    11401 aaaacaagag attatagcag aaattgaatc agactttgct gcaggatttg tggggattca
    11461 aaaacttcca attgaagcta aatttggtgt ttatacagcc tatatttatt ataaaaaatt
    11521 gttgtcaaaa ttaaaaaaga caccttcatt agaaataaaa aacactcgta ttagagtgcc
    11581 tgattacgaa aaagcaggac ttttagcaaa atgctatctt aattatcgat tgaatattat
    11641 ttaaactcaa cctattatgt ggattcatat tctcattttt tttgccacct ttttcatgat
    11701 ggaatttatg gcttggttta cgcataaata tattatgcac ggtattttat ggcatcttca
    11761 tgccgatcat cacaaaaaag accatgattc ttggtttgaa cgaaacgata ctttctttat
    11821 attttatgca atcgttagca ttagtttttt tctcttgtgg aaatatgaaa ttttagaact
    11881 aggtttggcc attggcttag gaattttagc ctacggatta acttattttt ttgtccacga
    11941 catctttatt catcagcgat ttaaactttt cagaaatgca aataatatat atgcaagagc
    12001 attaagaaga gcacacaaaa tgcatcataa gcacattgga aaggagcatg gagaatgctt
    12061 tggcatgctt ataattccct ttaaatattt caaataaaca gtattatttt actatttatc
    12121 tgtttataat ttcggtgtaa tttttaacag atttattctt aagttattct tataaaacat
    12181 agtttttcct atttattttt atttaattag cggaaaatta tttttatgaa tatcaaagta
    12241 actctacttc tttgtctttt tatcagcttt tcagtttttg gacaaaaaaa tattgatcct
    12301 actcctgctg atattgacct agcaaaatcg attagaagta aatatgaaaa atcagatgta
    12361 gctatccttg aaagtatgga gaatatcaca ttcggttaca ataaatcttc ttcaaatgta
    12421 actgtagatt tatcagtaga tgaattatta atgaatatca accatcgggc agatattttc
    12481 aagtatgaat tttatgatag ttcttctaaa attgaaaatt tcacaatcaa ataccgcaat
    12541 gataaattag cctattttgc cgttcaagat gagttttaca aagacaaaga tttattttac
    12601 aatgatgccc gtgtcaaaca tgtaaaaata gattttcctg ttcaagggta tacttataat
    12661 tatcatatcg ataagaaatt cgaagatgta aaatatttca cttctatcta ttttaatgat
    12721 gaatttccgg tgattaagaa aaaaataaaa gtcacaattc caaaatggtt gaatttagag
    12781 ttaaaagaaa tgaactttca aggacattct atcgtaaaaa cccaaacaac tgacgccaaa
    12841 aaaaacacga caacgattgt ttatgaattt aacgacattc ctgctttaat ttcagaagaa
    12901 aacagtcccg gtcgttctta tttatatcca catcttttag tcattgccaa atcatatgaa
    12961 atgaatggtg aaaaaatcac attgttcaat tcaactgccg atttatataa atggtacaaa
    13021 tcacttattg atcaaatgaa agatgaccct gagcaattaa aaagtaaagt aactgaactg
    13081 actgcgaaag ctaaaactga cgaagaaaaa ataaaaaaca tttattattg ggtacaagat
    13141 aatataagat acatcgcttt tgaagatggg attgcgggtt tcaaaccaga cgattcacaa
    13201 aacgtattca aaaaaagata tggtgattgt aaaggaatgg caaacctcat caaacaaatg
    13261 atgattattg ccggttttga tgctaggctt acatggattg ggacaaagca tattgcgtat
    13321 gattattcca taccctcact ttcagttgac aaccacatga tttgcacagt actatacaaa
    13381 ggaaaacaaa tctatttaga tggtactgaa aaaaatattt cattagagga atatgctgaa
    13441 agaattcaaa ataaagaaac cttaattgaa aatggtgata cttatataat cggcaaaatt
    13501 cctaccgtac aagcaagtca gaacaaggag acattccgtg caaaattagt agtagatggt
    13561 gaaaaactga aaggttcttg taaaagaacg tatttaggag aaagtcgcac tgaatttcaa
    13621 aacatttata atagttttga aacggataaa aaagcagaag cattagaacg atacctttct
    13681 aaaaacgaca aaaataatca tgtaacttct attaaaacaa gtgatttaaa gtctagggaa
    13741 caagctctaa caattgatta tgaactggaa tctgaaaacc gattaagtaa ctttgatggc
    13801 gaaatgtatt tgaactttga attcatgaat gaatataaaa aattcacttt aaaagaccgt
    13861 aaaacagatt atgaacttga ttataaaacg ttatatgatt ctgaaattac cattgtattg
    13921 cctgcaggat ataaagttga aaagctacct gaaaatttaa ttcaaaaaga agaagactat
    13981 caagtagaag tttctttctc acaaaaaggc aatgagcttc tttatcgcaa agtttttatt
    14041 ttcaacaatg caattttacc aaaaaacaaa caagaaaaat ggaataccac tcataaaaaa
    14101 cttgtcgaat tttataattc atccatcact ttaataaaat cataatttaa tgaaaaaaat
    14161 tattattctt tgccttttaa ttgcaaccac tatcaatgct caagataacg taaagattcg
    14221 cgacttcttt tggggaaaac aagacactca ttcaaaaacg gtggctattc ctgacaaatg
    14281 gaaaaatgaa tcagcagtta ttatttataa atttgatttt tatgattacc acaaattcgg
    14341 taaaaatgta aattatactt cggctaaacg aatcagggta aaattattag atgaagcagc
    14401 tgtaaaagaa ttttctgaat ttacttttac cgaacgtttt ttgtcaagta aaggttatgc
    14461 catgaaaaga ggcactaatt caattggtat aaaagtaata aaaccggatg gcaaagaaaa
    14521 tattttggat gttagtaagg aatctgtagt agctgataaa gaaaaaaaaa tagcaattcc
    14581 aaacttagaa attggtgata taattgatta ttacctgcat tcagaagaac ctttcaaatc
    14641 aatcatcgaa gtgggctttg atccggtaga aacaaatttg ggagatgtat atccgattat
    14701 ggaatacaaa ctgacttttc aaacagaaaa tgatttcttt gtaaatttta acacctacaa
    14761 tggtgctcca aaattaacac aaataccaac caaaaatagt ggagaacgca tctatgaatt
    14821 agcagcaacg aatcttgata aaaacgattt tccacgttgg ttttatcctt tagtagaatt
    14881 accatgctat aaatttcagg tgttttttgc ccgttccggt aaattcgagg aaagggcaaa
    14941 agcctttttg tcagagaaag aaaaagacgt aaaatcaacc gttacaaaag aagatatttt
    15001 caactattat gattcaaaat tccttgcgga aggaaatatt tctcaaataa atgacttctt
    15061 aaaaggtaag aagtttaaag atgacgaaga aaaaattaga gaagtatatt attataccag
    15121 acatcaatac ttcactcaat atgtggaggc atttgtggca aaagaagcca atatttttta
    15181 tccttttgag ctatatccca atgcaatatt ttttaataca gaagaacatt tcatcagaca
    15241 ttttatgttc tttttgaaga aaaatgacat ctcatatgat attgtggttg gaacagatcg
    15301 atataacggg gatatcaaag atttattaat tcaacagaat gtatctattt tgctaagagt
    15361 aaatacacct aaacctattt ttctggaatt ttttactcct tttacgagtg ccgaccaatt
    15421 cgattataat cttgaaaata ccaaagctta tttattacag gtttctaaag gcaaaaaaat
    15481 tattgatgca gaatcaataa cgttgccaag ttcaacgaaa aatgacaata tcaaccgatc
    15541 agttaccaaa atcagtttaa aagaagacct ttccagtttt aatgtaaatc gtgaacagtc
    15601 attatttgga cattataaag aaggtgaaca atcagataaa ttgtactttt ttgattatgt
    15661 atatgaagat tataaaaaat atggcaatac cccattaatg gaacttgtaa aaaacaaaaa
    15721 acaaagagct cagtacacaa aagaattcga tgccttaatt gcaaaatcaa aagaaaatca
    15781 aaaagaaagt tttattaaat ctgtaaaaga agaatttgaa attgacattg aaaactattc
    15841 gatggaaata aaaaatacgg gcagatttgg cagaaatgaa ccaatgcaat acacggaaaa
    15901 atttaccatt actgaccaat acatcaaaaa agccggaaac aatctaatgg tcgaattagg
    15961 gaaatttcta acatcgcaaa ttgagctttc taaaaaagaa aaagaacgta caaataatgt
    16021 ttacatgacg ttcccaagac aaattgaaca agaaattcaa tttgaaattc cggcgggtta
    16081 cactgtctcc ggacttgaaa aattcaataa aaaagtggaa aatgaaacag gaggttttgt
    16141 cagcactgct acacaaaacg gtaatctaat aactattaaa acgactaaat attattccaa
    16201 ttattttgaa ccaaacagca attggaaaaa aatggttgac tttttagatg cttcttacca
    16261 atttacacag gaaaaagttc tactaaagaa aaattaattc aaattagctc aaaaaaactc
    16321 aaaataaaca agtttgaatt tgtttgtttt gagtttttta
//
LOCUS       DAOTCW010000047        16354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2010_length_16354_cov_44.158783, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000047 DAOTCW010000000
VERSION     DAOTCW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 16354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16354
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2010_length_16354_cov_44.158783"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="P5335_05555"
     CDS             <1..384
                     /locus_tag="P5335_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74374.1"
                     /translation="QHFSEYVKFYGTTLFEETPIAFSFTTNIQDIESVKMNGQLRKHL
                     FYVIKEVFNNALKHAEATKTELKMQLLSPKLLQITIVDNGTGIIKENDYGNGIKNIQN
                     RIELLGGTFVMQTKNGLQTILTIRL"
     gene            637..4038
                     /locus_tag="P5335_05560"
     CDS             637..4038
                     /locus_tag="P5335_05560"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRZ74375.1"
                     /translation="MKKLILHSFLFFSIGLFAQVGINTTTPQAQLEIKSSNEATPSNT
                     DGILIPKIDTFPATNPTLAQQGMLVYLTIPVGTNLPGFYYWDDATSSWINLKGADGGT
                     LDQAYDFGGAGLGRTVTADNGAVLINGTDGLVSTGTLGSGALAPSGAGTRMVWNPRKA
                     AFRAGETTGAQWNDVNIGVISTAFGFNAIASGNFSTAFGGNTIASGATSTAFGSTTIA
                     SGFRSTVFGYENTSSSHGETVIGIGATTYTPSTNGDTQFRTANSTDRLFVVGNAIDVN
                     NNSTVDVAERSDAMVVLKNGLTRLPSTTNTMIDAADGKAVVTKEYLQNNSSGTLDQAY
                     DFGGAGNGRTIIADTGAVTITGTDGFVSTGTIGSGAIAPSGDGIRMVWNPRKAAFRAG
                     NVNTINWDDANIGTSSVALGNNPRASGSASVAIGNVVTASGIGSSAFGTSSSAIGQAS
                     TVFGRNNIAFASESTAFGFNNSASSYGETVIGIGATNYTPSINGALQFRTANETDRLF
                     VIGNAIDANNNNTVDVAERSDAMVVLKNGNVGVGLSTPQNRLHFHNPTGIQTFIQLTN
                     SSTGITQNDGMILGNIIGTSEAGIINQEPTNFYLGTSSDQNQLVLTPSGNVGVGTDSA
                     QAKLHVEGNIRMVDGNQAAGRVLTSDANGTATWTTPGVGTAGTLDQSYDFGGAGLGKT
                     ITADAGAVTINGTDGFVSTGTDGIGALVPSGLGTRMVWNPRKVAFRAGRAIGTEWDDA
                     SIGRYSVAFGYNSTASGLYSTAFGGFSTASGQFSTAFGNNALASGYASTAIGELATAS
                     GTYSRCFGWSSLASGTISTAFGASTIASGNISFVSGNGNSAPSYGETVIGIGATIYTP
                     SVNGAQLFRIANETDRLFVIGNAIDTNNNSILEDSERSNAMVVLKSGNTGIGISSPQE
                     RLQVGIGNIAVTQGDIYLAALNGIFNAGGGLMTNAINYIGDAFVPPTIVAGDEDLYIA
                     DDIELGGQGYKPGGGTWVAPSDRRLKQNIQSYSDGLESVLRINPVTYQYNSVYKTLDT
                     GETYVGVIAQEVQEVAPYMINEKPFGQVVEEDENGQERIVKQGTNYLTFDSSALTYML
                     INAVKEINGKYEELRTKNETLENKINELLSKMEMLEKKYFLNDGK"
     gene            complement(4511..4942)
                     /gene="umuD"
                     /locus_tag="P5335_05565"
     CDS             complement(4511..4942)
                     /gene="umuD"
                     /locus_tag="P5335_05565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HRZ74376.1"
                     /translation="MTKTKLTFFIPDLDNPIELPFITAGIKAGFPSPAADFDETKISL
                     DAVLVKNREATFYAKATGTSMIGAGIDDEDILVIDRSLEPRNNKVAVCYIDGEFTVKR
                     IKVTKDGVLLMPENKAFEPIKITEENQLIIWGIVTHVVKRL"
     gene            complement(4997..6262)
                     /locus_tag="P5335_05570"
     CDS             complement(4997..6262)
                     /locus_tag="P5335_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HRZ74377.1"
                     /translation="MYALVDCNNFYASCERVFQPQLNGKPIVILSNNDGCIISRSDEA
                     KALGIAMGAPEFKVRDELKQKNITVFSSNYALYGDLSSRVMAILGQFTPNMEVYSIDE
                     AFLNFDGMKIDDYQQYGLEMKKRTLKWLSIPVSVGFAPTKALAKVANKIARKFPNQTL
                     GSYVIDTEEKRIKGLKWTKIEDVWGIGFRLTKKMKAQNIHTAFDFTLPHNETFIKKEM
                     GVVGNRLRLELLGQSVLELEEPKDRKNIAITRSFDGNITTFDEMKERVSTFATVCAEK
                     LRKQKSCCYGVILYLRKDKYKVDKPKYNFYKMESLPFASNSSITISAVAIKMLKEIFE
                     EGEMYKKAGVIVTQIIPQDQKQFHLFEEENPKHQKIMEVMDAFHKKTGERKIRLGNQD
                     LQRTWKMKQNLLSPKYTTDIRDIFKVNCG"
     assembly_gap    6340..6439
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6440..6915)
                     /locus_tag="P5335_05575"
     CDS             complement(<6440..6915)
                     /locus_tag="P5335_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRZ74378.1"
                     /translation="MNYHFRKATITDAPKIWIILQQAILRRKIDGSNQWQDGYPNPQI
                     IQQDIEKGIGYVLTDDNIIIGYTAVLINNEPEYANLQGKWITNSDFVVFHRVAIAENH
                     LGKNLSKKIIDFIEKFALTNNIYILKADTNHDNFAMMSIFEKSGFVFCGIVHFRGSP"
     gene            complement(7168..7365)
                     /locus_tag="P5335_05580"
     CDS             complement(7168..7365)
                     /locus_tag="P5335_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRZ74379.1"
                     /translation="MRTGTVKFFNESKGYGFITDEETGKDIFVHATGIKAEELREGDR
                     VSYEEEEGRKGKVAAQVAVIE"
     gene            complement(7451..7684)
                     /locus_tag="P5335_05585"
     CDS             complement(7451..7684)
                     /locus_tag="P5335_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74380.1"
                     /translation="MKYIIRILFIIILGLIGYGYFLKQTVGNEGDKWVGLGVLISALV
                     LMPIFLWHRYKNKKLEDYMFKFDNKKDEKTENQ"
     gene            complement(7689..9440)
                     /gene="aspS"
                     /locus_tag="P5335_05590"
     CDS             complement(7689..9440)
                     /gene="aspS"
                     /locus_tag="P5335_05590"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRZ74381.1"
                     /translation="MYRSHTCGELNSSHINTEVTLAGWVQKSRDKGFMNWIDLRDRYG
                     ITQLIFDENRTEKSVFEASKSLGREFVIQVKGTVIEREAKNKNIPTGEIEILVCELSI
                     LNVAQLPPFTIEDDTDGGEDIRMKYRYLDIRRNPVKNSLIFRHKVSMEVRNYLSNQGF
                     CEIETPYLIKSTPEGARDFVVPSRMNEGQFYALPQSPQTFKQLLMVGGMDKYFQIVKC
                     FRDEDLRADRQPEFTQIDCEMAFVEQEDILNVFEGLTRHLLKEIKGIEVDKFPRITYD
                     YAMKTYGNDKPDIRFEMKFGELNAVAQHKDFPVFNSAELVVGIAVPGCASYSRKEIDS
                     LIDWIKRPQIGATGMVYVKYEVDGSLKSSVDKFYDEADLKKWAEITNAKPGDLILILS
                     GKADKTRTQLSALRMEVATRLGLRKPEEFAPLWVVDFPLLEWDEESQRYHAMHHPFTS
                     PKPEDMKLIETEPGKVRANAYDMVLNGNEIGGGSIRIHDRDLQSRMFSLLGFTPEQAE
                     NQFGFLMNAFQFGAPPHGGLAFGLDRLVAILGGQETIRDFIAFPKNNSGRDVMIDAPS
                     VIDESQLKELHIKLDLK"
     gene            9585..10583
                     /locus_tag="P5335_05595"
     CDS             9585..10583
                     /locus_tag="P5335_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRZ74382.1"
                     /translation="MKNKLQFLGITILIVFTTFTSVAQMTFTAKIENRVSDTITIRGA
                     QKFIKKIAVDNKGIFTDTFEVTTGFYQFFDGSEYTQLYLKNGYDLLMTLNTKEFDESI
                     AYKGNGSLENNVLAKKALDDEQFELKHFTTDDETAFQNAFDTKKASDAKLLSDEKLDE
                     NFRTVMTKRASQEYGMVVEMRESAMAAKKMVGKPSPTFTYENHKGGTSTLADFKGKYV
                     YIDVWATWCGPCLAEIPHLKKVEAAYHDKNIVFLSISIDVKKDYEKWKNMVNQKQLGG
                     VQLIADNDWNSKFVTDYGIQGIPRFILIDPNGNVVEADASRPSSPDLLKKLDVLLK"
     gene            10763..>11106
                     /locus_tag="P5335_05600"
     CDS             10763..>11106
                     /locus_tag="P5335_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-binding protein"
                     /protein_id="HRZ74383.1"
                     /translation="MKKLFIAAMLVVSGVIFAQENNVKNEIVNKMVKSTYYYDNGQVQ
                     QEGFYKDGKVHGEWISYDINGNKVAMGQYQNGMKVGKWFFWNGLDLAEVDYSDSRVAQ
                     VKKWSQGAVVQV"
     assembly_gap    11107..11206
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11207..11556)
                     /locus_tag="P5335_05605"
     CDS             complement(<11207..11556)
                     /locus_tag="P5335_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74384.1"
                     /translation="MKKVITLLAVLLFFSNCQVDTETQNIKVKNQYSIDLPKSLASVD
                     FLHEDASLQYQNILSEFYTIVIDEPSSDFDNLVLEDASLRETYTPDLTGYSKLLKDNL
                     AATIKNGKFSKFEDT"
     gene            complement(11612..>14209)
                     /locus_tag="P5335_05610"
     CDS             complement(11612..>14209)
                     /locus_tag="P5335_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRZ74385.1"
                     /translation="MLDIKKRSGKNMVEAVEGLKAIVDKAKENYFPEGLTITYANDQS
                     TRTIAQVDDLVNNIIFGVILVIGVLMFFLGFRNAIFVGFAIPLSMFMSYMILASFGIT
                     LNTMVLFGLVMGLGMLVDNGIVVVENVYTLMSQGMSRKQAAIEGIGEIAWPIIASTAT
                     TLAAFFPLGLWPGTMGKFMIYFPMTLSVVLFSSLFVALIINAMLTADFMKLKEEELSK
                     KALIKLSGFVGVIGLVMLVAGFNTKSGGLKGLGNLMLFFAIMLWIYKYYLVRAQDFFQ
                     RIILVWMETKYRNFLQYALTGKKPLVFFFGTFLLLILSFVAVGIAKPNVLFFPENQPN
                     QIMVYIEFPEGTDIKKTNAFVKQIEQKVYAVANKYMEDGENYMVESAIAQVGNGSGNP
                     QTDGGSENDMPHRGKITLTMREFKYRKGLNSLDVMEEIRTAVQGYAGVSVIVEKEQNG
                     PPAGYPVNIEISGEDYNAMLLEAQKMKEYINEKNIAGIEELKIDVNKAKPGVDVKIDL
                     DKAGQLGITSGQVGQTLRRAIFGEKISTYKDGKDDFEINVRLPEGQRNDNKLIYNQPI
                     TFRDQATGKLVQVPISAITRSENSTTFNTIKRKNLKRVITVYSNVITGYNGNEVVEKI
                     KREMEDYQIGNDINFAFTGEQEEQSKNMGFLVNALLIALGGIILILVAQFNSISKPII
                     IMVSVLLSFGGVFFGMAFFGDDFVIIMTMMGIISLAGIVVNNAIVLIDYTQLLIDRKL
                     VEKGLDEKGLLTKHEYLDLIVEGGRSRLRPVLLTAITTVLGLVPLAIGLNIDFFGLFI
                     DYNPNIYMGGDNVIFWGPLAKTVIYGLTFATFLTLVIVPVMFFLLHKGKIKVKKKKKK
                     NINQILQTN"
     assembly_gap    14210..14309
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14310..15156)
                     /locus_tag="P5335_05615"
     CDS             complement(<14310..15156)
                     /locus_tag="P5335_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRZ74386.1"
                     /translation="MSLNIHKNFAISTWAINNKMTVYVITAILLVGGLISYYSMPRES
                     FPEIIETKIYVSSINPGNSAEDVEKLITKPLEEEFNNISGVTKITSNTLEDYSMILVE
                     FDEDISVEDAKQKVKDKVDRVKSDTEWPTIDGGGKVEPNVFDLNIAEEVPILNINLTG
                     DFTSEKLKEYAEYLEEHIERLPQIKEAAIRGAEDKEVEIAVDMYKMSAAKVSFDDIIS
                     SVKYENKTVSGGSVSTNGIKKNIRVIGEINKPSDLENIIVKKQDGNVYLRDIATIEFK
                     EKERTT"
     gene            complement(15177..16346)
                     /locus_tag="P5335_05620"
     CDS             complement(15177..16346)
                     /locus_tag="P5335_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRZ74387.1"
                     /translation="MKKILSITVLTLLLAACSGENKTGNLDEVIQSKDLAKIKSSRES
                     VHKEYEKLVADLAKLDKAIAELDPNKKNPLVQTITLKDTLFTHYIEIQGNVDTKENIM
                     ISPEYSGILTQVNVKAGQRVSKGQVLARIDDGGMSSQLAQAETQLALARTTFEKQKNL
                     WDQKIGSEIQYLQAKTSMESQQKMVAQIKSQLNKTVVTAPFSGTIDEVLTEKGKVVAP
                     GMELFRIVNLNNMYVTANVPENYIGQLKVGAVVDVHLNSLGKNYKGKVRQVGNYINPN
                     NRTFGIEIALPNPDNLLRPNQVAVLKIEDYKNQKALLLPENIIQETADGSKVIYVIDK
                     VDSKNNAMATQKTIEIGYTSGAFVEVKSGLQSGEIVITEGGKSLKDGQTVEVIKL"
BASE COUNT         4941 a         2843 c         2965 g         5305 t
ORIGIN      
        1 caacactttt cagaatatgt taagttttat ggcacaacac tttttgaaga aacaccgatt
       61 gcattttcat ttaccaccaa cattcaggat attgaatcgg taaaaatgaa cgggcaatta
      121 cggaagcatt tattttatgt catcaaagaa gtttttaata atgcgttgaa acatgctgag
      181 gcaaccaaga cagaattgaa aatgcaattg ctttctccta aattgttaca aattactatt
      241 gtggataatg gaaccggaat catcaaagag aacgattatg gaaatggaat taaaaatatt
      301 caaaatagaa tagaactatt aggaggaacg tttgtcatgc aaactaaaaa tggcttacaa
      361 accatactta ccattcgact ttaaaattac ttttttaatt tgtaagaaaa taggaatacg
      421 ctaaaaggcg tattttttta tgcactccaa atgcgttaag ttttgataca atattgattt
      481 atttgataaa gccttgtggt tactgacttt tttattatta gaactgttaa aataactacg
      541 gataatatcg tattgtggta tccatttgat tgagtgaatt ttatacttca gaaatcacta
      601 aaagcagctt attctgtttt ttcaaaaacc actatgatga agaaattaat tttacattca
      661 ttcttgtttt tttccattgg cctctttgca caagtgggaa tcaataccac cacacctcaa
      721 gctcaattag agattaaatc atccaatgaa gcaacaccaa gtaacaccga tggaatttta
      781 attcctaaaa ttgacacttt tccggctacc aatccaacgc ttgctcaaca agggatgttg
      841 gtttatttga ctataccggt aggaaccaat ttacccggtt tttattattg ggatgatgcc
      901 acatcgagtt ggataaattt gaaaggagct gatggcggaa cgctcgacca agcgtatgat
      961 tttggcggag ctggtttggg tagaactgta actgccgata acggagctgt gttaatcaat
     1021 ggaacggatg gattggtttc aactggaacc ttgggcagtg gtgctttagc tccttcaggt
     1081 gccgggacaa gaatggtttg gaatccaaga aaagctgcat ttagagcagg tgagacaact
     1141 ggagcacaat ggaatgatgt aaatattggt gttatatcaa cagcttttgg ttttaatgct
     1201 atagcatcag gaaatttttc aacagctttt ggaggtaata caatagcatc gggggcaact
     1261 tcaacagctt ttggttcaac cacgatagca tcaggatttc gatcaacagt ttttggttat
     1321 gaaaatacat cgtcaagtca tggtgaaaca gtaattggta ttggagcaac tacttacacc
     1381 ccatcaacca acggagacac acaatttaga actgccaact caacagatag attatttgta
     1441 gtaggaaatg caattgatgt taataataat tctactgttg atgttgctga aagaagcgat
     1501 gcgatggtgg ttttaaaaaa tggcttaacc cgtttgcctt caacaactaa tacaatgata
     1561 gatgctgctg atggaaaagc agttgttaca aaagaatatt tacaaaataa ttcatctggc
     1621 acacttgacc aagcatacga ttttggagga gcgggaaatg gtagaacgat aattgcagat
     1681 acaggcgcag taactattac cggaactgat ggttttgtat ctacaggaac tataggaagt
     1741 ggtgctatag ccccttcagg tgacggaata agaatggttt ggaatccaag aaaagcagca
     1801 tttagagcgg gaaatgtaaa taccataaat tgggatgatg ctaatattgg gacatcttca
     1861 gtagcccttg gtaataaccc tcgtgctagt ggatctgctt ctgtagcaat tggaaatgtt
     1921 gttacggcat cgggaatagg ctcttccgcc tttggaacta gttcttctgc gattggtcag
     1981 gcttcaacag tttttgggag aaataatatt gcctttgcta gtgaatctac ggcttttggt
     2041 tttaataatt ctgcatccag ttatggtgaa accgttatag gaattggagc aacaaactat
     2101 actccaagta tcaatggtgc tttacaattc agaactgcca atgaaacaga ccgtttgttt
     2161 gtaattggaa atgccattga tgctaacaat aacaataccg ttgatgtagc agaaagaagc
     2221 gatgcgatgg tggttttgaa gaatggaaat gttggtgtag ggttatctac accgcagaat
     2281 agattgcatt ttcataatcc gacaggtatt caaactttca ttcaattaac taattcttct
     2341 acaggaatta ctcaaaatga tggaatgata ctaggaaata ttatcggtac aagtgaagca
     2401 ggaatcatta atcaagagcc aacaaacttt tatttaggaa catctagtga tcaaaatcaa
     2461 ttagtattaa caccctcggg aaacgtaggc gttggaactg attcagcaca agctaaactt
     2521 cacgttgaag gaaacatccg tatggtagac ggcaaccaag ccgcaggaag agttttaaca
     2581 agtgatgcga atggaacggc aacatggact acgccgggag tgggaactgc tggtacatta
     2641 gaccaatctt atgactttgg tggagcgggt ttaggaaaaa caataactgc agatgccgga
     2701 gcggttacta taaatggcac agatggtttt gtttctactg gcacagatgg gattggagcc
     2761 ttagtcccaa gtggattagg aacaagaatg gtttggaatc ctagaaaagt tgcttttaga
     2821 gctggaagag cgatagggac agaatgggat gatgctagta ttgggcgata ttccgttgct
     2881 tttgggtata atagtacagc aagtggttta tattcaactg catttggtgg attttcaaca
     2941 gcaagcggac aattttcaac ggcttttggg aataatgctt tggcaagcgg ttatgcatct
     3001 actgctattg gagaacttgc aactgcaagt ggaacatatt caagatgttt tggatggtca
     3061 tctttggcaa gtggtactat ttccacagca tttggtgctt ctactattgc cagtggtaat
     3121 atatcctttg tttcaggaaa tgggaatagt gctccaagtt atggggaaac agttatcggt
     3181 attggtgcta caatatatac gccttcagta aatggagccc aactatttag aatcgctaat
     3241 gaaacagata gactttttgt cattggcaat gcaattgata ccaacaataa tagtatactg
     3301 gaagatagcg aaagaagcaa tgcaatggta gttttaaaaa gtggaaatac aggtattggt
     3361 atatcttctc cccaagaacg attgcaagtt ggtattggaa atatagctgt aactcaaggc
     3421 gatatttatt tagcggctct caatggaatt tttaatgctg gcggagggtt gatgacaaat
     3481 gccatcaatt atattggtga tgcttttgta ccaccaacta tagtagctgg agatgaagat
     3541 ttatacatag ctgatgatat tgagttagga ggacaaggtt ataaacccgg aggaggaaca
     3601 tgggtggctc cttccgatag aagattaaaa caaaatatac aatcttattc cgacggatta
     3661 gaaagtgttt taaggatcaa tccagtcacc taccaatata atagtgtcta taagacattg
     3721 gatacaggtg aaacctatgt aggggtaata gctcaagagg tacaggaagt agctccttat
     3781 atgataaatg aaaaaccttt tggacaagtt gttgaagaag atgaaaatgg acaggaaaga
     3841 atagtgaaac aaggaacgaa ttacttaaca tttgatagct cagccttaac ctatatgttg
     3901 atcaatgcag taaaagaaat aaatggtaaa tacgaagaat taagaaccaa aaacgaaacc
     3961 ctcgaaaata aaataaacga attattaagc aaaatggaaa tgttagaaaa aaaatatttt
     4021 ttaaatgatg gaaagtaata taatcacatc cttattattg attgtattta tcagttttat
     4081 agtttttctc aaaacaatta accacactaa attataatct ttcaaaacca ctaagagaat
     4141 aagtagtgag agatttggtt cttcctgaga attccattgc atagataaaa gcaccacttt
     4201 gcaaaactct ttgatacaca aatcaattca acagtttttt tgatattaac aataagggta
     4261 atgaaaacga ttccgtaacg ttagtagtgc agacaacaaa cagaactttc aatgcggtca
     4321 tttctataga tacacagtca ggtgttttta taattaatag agtgagaaag gtttatttca
     4381 ccaattagaa aagaccaagc aacaaaacaa atcaaacggt agtaagcgtt agaacggtaa
     4441 tcatttggtt ttaaattagt tttagtggtt gatccccttc cggcaagtgg tttgcggagg
     4501 gggatctttt ttaaagtctt ttcaccacat gtgtcacaat cccccaaatg atcaattggt
     4561 tttcttcggt aattttgata ggttcaaaag ctttattttc cggcatcaaa agtacaccgt
     4621 ctttggtcac tttgatgcgt tttacggtga attctccatc aatataacaa acggctactt
     4681 tgttgtttct gggttccaaa cttcggtcaa tgactaaaat gtcttcatca tctattcccg
     4741 ctccaatcat ggaggttcct gtggctttgg cataaaatgt tgcttctctg tttttcacta
     4801 aaacagcatc taaactgatt tttgtttcat caaaatcggc tgccggtgaa ggaaatccgg
     4861 ctttgattcc ggctgtaata aacggaagtt caattggatt atctaaatcc ggtatgaaaa
     4921 aggtaagttt cgtttttgtc atattttttt aaccacaatg agcacaaaga aggcacaaag
     4981 ttcacaaaat taagcattac ccacaattta ctttaaaaat atcccgaata tccgtcgtgt
     5041 attttggcga aagcaaattc tgtttcattt tccaagtgcg ttgcaagtct tggttaccta
     5101 acctgatttt gcgttcgccg gttttcttgt gaaaggcatc catcacttcc atgatttttt
     5161 ggtgtttcgg gttttcttct tcaaacaagt gaaattgctt ttggtcttgt ggaatgattt
     5221 gggtaacgat gactccggct tttttataca tttcaccttc ttcaaaaatt tctttcagca
     5281 ttttaatagc cactgcactg atggtgatac tggaattact cgcaaaaggc aagctttcca
     5341 ttttataaaa attatatttt ggtttatcga ctttgtattt gtcttttcgc aagtaaagaa
     5401 ttacgccata acagcaagat ttttgtttgc gaagcttttc tgcacaaacg gtagcaaagg
     5461 tagaaacccg ttctttcatt tcgtcaaacg tggtaatgtt gccatcaaaa cttcgggtga
     5521 tggcgatgtt ttttcggtct ttaggttctt ccaattccaa aacagattgg cctaataatt
     5581 ccaatcgcaa gcggttgccc accacgccca tttctttttt gataaaagtt tcgttatgtg
     5641 gtaatgtaaa atcgaatgct gtatggatat tttgagcttt catttttttt gtgagtcgaa
     5701 agccaatacc ccaaacgtct tcaattttgg tccattttaa tcctttgatg cgtttttctt
     5761 cggtgtctat tacataactt ccgagtgttt gatttggaaa tttacgggca attttgttgg
     5821 ctacttttgc taaagctttg gtaggagcaa agccaacgga aaccggaata ctcaaccatt
     5881 ttaacgttcg ctttttcatt tccaggccat attgctgata atcgtcaatc ttcattccat
     5941 caaaatttaa gaatgcttcg tcgatgctgt aaacttccat attgggcgtg aattgtccta
     6001 aaattgccat gacgcgacta ctcaaatcgc catacaaagc ataattagaa gaaaaaacgg
     6061 tgatattttt ttgtttgagt tcgtcacgta ctttaaattc aggagctccc atcgcaatac
     6121 ccaacgcttt ggcttcgtcg ctgcgggaga tgatgcagcc gtcgttatta gacaaaataa
     6181 cgattggttt tccattcagt tgaggttgga aaacacgttc acaggaagca taaaaattgt
     6241 tacaatctac taaagcatac atgctgtaaa gttagtagat tattgttttt tttggaagtg
     6301 aacggatgtt aatgttgata agaagttaga ggcaagaaan nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnng gactacctct gaaatgcacg atgccacaga acacaaaacc
     6481 tgatttttca aaaatgctca tcatggcaaa attgtcgtgg ttggtgtcgg ctttgaggat
     6541 gtaaatgtta ttggttaaag caaatttttc aataaaatcg attatttttt tcgataaatt
     6601 tttacctaaa tgattttctg cgatggctac tcgatgaaaa acgacaaaat cgctattcgt
     6661 aatccatttt ccttggagat tggcgtattc cggttcgttg ttgattaaaa cggctgtata
     6721 accgatgatt atattgtcgt cagtaagtac ataaccaatt cctttttcaa tgtcttgttg
     6781 gatgatttgt gggttaggat aaccgtcttg ccattggttg cttccgtcga tttttcttct
     6841 aaggatggct tgctggagaa ttatccaaat tttgggagca tctgtgatag tggcttttcg
     6901 gaagtggtag ttcatggaag tttgtgttct gtatactaga aaataagatg taaagttagc
     6961 agaatttttt attgaagcac agcttgcgtg agggatagag cccttgtttg agctctcccg
     7021 atttttatcg ggaagcgagt ggcgaaagcc cgacccttgt gggcacgccc aaaaaccaat
     7081 tttataaatc cgtgttcttt gaaatacaaa aaacgtctcg attgctcgag acgtttgtca
     7141 ttcattcggt aatctaaata atgtatatta ttcgatcaca gctacttgag ctgctacttt
     7201 accttttcta ccttcttctt cttcgtaaga aacacggtca ccttctctta gctcttcagc
     7261 tttgatacca gttgcatgaa cgaaaatgtc ttttcctgtt tcttcatctg taatgaatcc
     7321 gtaaccttta gactcattga aaaattttac tgtacctgta cgcattgtga taataaaaat
     7381 aattaataat ggccaaaggt aatctataaa tacatacgaa tgttaattca atactatttt
     7441 tttgtgaaat ttattgattt tcagtctttt cgtctttttt attgtcaaat ttgaacatat
     7501 agtcttccaa tttcttgttt ttatagcgat gccataaaaa aatgggcata aggactaatg
     7561 cactgattaa aactcctaat ccaacccatt tatctccctc gtttccaaca gtttgtttta
     7621 aaaaatagcc gtagccaata agtccaagga tgataataaa cagtattcgg atgatgtatt
     7681 tcatggaatt attttaaatc taatttaatg tgtaattctt ttaattggct ttcgtcaatt
     7741 acggaaggag catcaatcat cacatcgcga ccactgttat ttttagggaa cgcgatgaaa
     7801 tctcgaatcg tttcctgtcc acctaaaatt gctactaaac ggtctaatcc gaacgctaaa
     7861 ccaccatgtg gcggagctcc aaattggaag gcattcatta agaatccaaa ttgattttct
     7921 gcttgttccg gtgtgaaacc taacaaagag aacattcgac tttgtaaatc acggtcgtga
     7981 atacgaatag aacctccacc aatttcattt ccgttcaata ccatgtcgta ggcattggca
     8041 cgtacttttc ccggttcggt ttcaattaat ttcatgtctt ccggttttgg tgaagtgaac
     8101 ggatggtgca tcgcatggta gcgttgcgac tcttcgtccc attccaacaa aggaaaatca
     8161 actacccaaa gtggagcaaa ttcttccggt ttacgcaagc ctaaacgagt agctacttcc
     8221 attcgaagtg ccgataattg agttctggtt ttatcagcct ttccggaaag gattaaaatt
     8281 aaatcacccg gtttagcatt tgtaatttca gcccattttt ttaaatcggc ttcgtcataa
     8341 aatttgtcaa ccgatgattt taaacttccg tctacttcat attttacata gaccattcct
     8401 gtagctccga tttgcggacg tttaatccaa tcaattaaag agtcaatttc ttttctggaa
     8461 taggaagcac aacccggcac agcaattccg acaaccaatt cagcggaatt aaagaccgga
     8521 aagtctttgt gttgagcaac agcatttaac tctccgaatt tcatctcaaa acgaatatcc
     8581 ggtttgtcgt ttccgtacgt tttcatggca taatcgtagg tgattcttgg gaatttatct
     8641 acttcgattc ctttgatttc ttttaataga tgacgcgtca atccttcaaa aacattcaga
     8701 atatcttctt gttctacaaa cgccatttcg cagtcgattt gggtgaattc cggttgacgg
     8761 tcggcacgca aatcttcatc tctaaaacat tttacgattt ggaaatattt atccattcca
     8821 cctaccatca acaattgttt gaaggtttgt ggcgattgtg gtaatgcgta gaattgtcct
     8881 tcgttcatgc ggcttggcac cacgaaatct ctagctcctt ccggagtaga tttgatcagg
     8941 taaggtgttt caatttcaca aaaaccttgg ttggaaagat aattacgtac ttccatcgaa
     9001 actttgtggc ggaaaatcaa actgtttttt accggattac ggcgaatatc taagtagcgg
     9061 tatttcatac gaatgtcttc cccaccatcg gtatcatctt caatggtaaa gggtggcaat
     9121 tgtgctacat tcaaaataga aagttcacaa actaagattt ctatctctcc ggttggaata
     9181 tttttatttt ttgcttcccg ttcaatgacc gttcctttta cttgaatgac aaattcacga
     9241 cccaatgatt tggaagcttc aaaaactgat ttttcggtac gattttcatc aaatattaat
     9301 tgtgtaatgc cataacgatc acgtaaatca atccaattca taaatccttt atctcttgat
     9361 ttttgtaccc aaccggcaag ggttacttcg gtattgatgt gcgagctgtt taattctccg
     9421 caggtatgac ttctatacat tgaaaataaa atttttgcaa aagtaaaaca aaaatccaat
     9481 acttgtagta tttcaacagt tgctctacta cttaatttca attatttttt gatttgatat
     9541 ttaaaaaaac gagtatattt gatttactta aatggtattt aatcatgaaa aacaaactac
     9601 aattcttagg aattacaatt cttattgttt ttaccacttt tacatcggta gcacaaatga
     9661 cttttactgc aaaaattgaa aatcgtgtca gtgatacgat tactattcgt ggagctcaaa
     9721 agttcataaa aaaaatagcc gttgataata aaggaatttt tacagacacg tttgaagtta
     9781 ctaccggttt ttaccaattt tttgacggta gtgaatacac acaattatat ttaaaaaacg
     9841 gttatgattt attaatgact ttaaacacta aagaatttga tgaatctatc gcttacaaag
     9901 gaaacggttc gttagaaaac aatgtcttag caaaaaaggc tttagatgat gaacaatttg
     9961 aactgaaaca ttttacaact gatgatgaaa ctgcttttca gaatgcattt gacacaaaaa
    10021 aagccagtga tgcaaaactt ttgagcgacg agaaattaga tgaaaatttc agaactgtca
    10081 tgaccaaaag agcaagtcaa gagtatggaa tggttgtaga gatgcgtgaa agtgctatgg
    10141 cagccaaaaa aatggtaggt aagccatcac ctacttttac ctatgaaaac cacaaaggag
    10201 gaaccagtac attggcagac tttaaaggaa aatatgttta cattgatgtt tgggccactt
    10261 ggtgcggacc ttgtttagct gaaattccgc acttgaaaaa agtggaagca gcttatcatg
    10321 ataaaaatat tgtgtttttg agtatctcca ttgatgtaaa aaaggattat gaaaaatgga
    10381 aaaacatggt gaatcaaaaa caattgggtg gtgtacaatt aattgccgat aacgattgga
    10441 attccaaatt tgttactgat tatggaatac aaggaattcc tcgctttatt ttgatagatc
    10501 cgaatggaaa tgttgtggaa gcagatgctt caaggccatc ttcgcccgat ttgctaaaaa
    10561 aattagatgt tttgttaaag taaataaatt tacataatta ccttaaaaac aacgcgttag
    10621 cgttgttttt tttgttttta ttcaattttc caatgttaag ttttcatcta atgttaccaa
    10681 attgttacca aattatttct taaatgtaaa tttatttata tatttgtaat gtaattgtta
    10741 acctacttaa aacgaagaag atatgaaaaa gttatttatt gcagcaatgc tagtagtttc
    10801 aggagtaatt tttgctcaag aaaacaatgt gaaaaatgag attgtaaata agatggttaa
    10861 atcaacttat tattatgaca atggtcaagt gcaacaagaa ggattctata aagatggaaa
    10921 agtacatgga gaatggattt cgtatgatat aaacggaaat aaagtagcca tgggtcaata
    10981 ccaaaatggg atgaaagttg gcaaatggtt cttttggaat ggattggatt tagcagaagt
    11041 agattattct gatagccgag tggctcaagt gaaaaaatgg tctcaaggag cagttgttca
    11101 agtaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtgt cttcaaattt
    11221 tgaaaatttt ccatttttaa ttgttgccgc taaattgtct tttaacaact tggaatatcc
    11281 tgtcaaatcg ggagtgtaag tttctcttag cgaagcatcc tccagtacta aattgtcaaa
    11341 atcagaactg ggttcgtcga taacgatagt ataaaattca cttaaaatgt tttggtattg
    11401 gagggatgca tcttcatgta aaaaatccac actagctaat gattttggca aatcgattga
    11461 atattgattt tttactttta tattttgagt ttctgtatca acttgacaat tactgaaaaa
    11521 gagaaggaca gctaataaag ttataacttt tttcatacgt attatttttt gcaagataaa
    11581 aaaaaaccac gctttcacgt ggttttaaag gctaatttgt ttgtagaatt tgattgatat
    11641 tttttttctt tttctttttg actttaattt ttcctttatg caataaaaag aacatcaccg
    11701 gaacaataac taaggttaag aaagtggcaa atgttaatcc gtaaataact gttttggcca
    11761 gcggtcccca gaatatcaca ttatcgccac ccatataaat gttaggattg taatcaatga
    11821 ataaaccgaa aaagtcaatg ttcaaaccaa tcgccaaagg caccaaacca agaacggttg
    11881 taattgccgt taataaaacc gggcgtaatc gcgagcggcc accttcaact attaaatcta
    11941 aatattcgtg tttggttaac aatccttttt cgtccaaacc tttttcaact aattttcgat
    12001 cgattagtaa ttgggtataa tcaatcaata caatggcgtt gtttacgaca attcccgcta
    12061 aggaaataat tcccatcatg gtcattataa tgacgaaatc atcaccaaaa aaggccattc
    12121 caaaaaacac accgccaaag cttaacaaga ccgaaaccat gatgataatt ggtttggaaa
    12181 tggaattaaa ctgagctact aagattaaaa taattcctcc aagtgcaatc aataaggcat
    12241 ttaccaagaa tcccatgttt ttggattgct cttcttgttc acctgtaaag gcaaaattaa
    12301 tgtcatttcc aatttgataa tcttccatct cccgtttgat tttttctacc acttcattgc
    12361 cattataacc cgtaattacg ttagagtaaa cagtaatcac tctttttaag tttttacgtt
    12421 taatagtatt aaatgttgta gaattttcgc ttcttgtaat tgctgaaata ggcacttgta
    12481 ccaatttacc cgttgcttga tctctgaaag taatgggttg gttataaatt aatttgttat
    12541 catttcgttg tccttccggt aaacgaacgt tgatttcgaa atcatctttt ccatctttat
    12601 aagtagaaat tttttcacca aaaatggctc tacgcaatgt ttgtcccact tgtccggaag
    12661 taattccgag ttgtccggct ttgtctaaat caatttttac atctacaccc ggttttgctt
    12721 tgtttacatc aattttcagt tcttcaatgc ctgcaatgtt tttctcattg atatactcct
    12781 tcattttttg agcttccaac aacatggcat tgtaatcttc ccctgaaatt tcaatattca
    12841 ccggataacc ggcaggcgga ccgttttgtt ctttttccac aatgactgaa acacctgcat
    12901 acccttgaac agcggtgcga atttcttcca tcacgtctaa actgttcaac ccttttcggt
    12961 atttaaattc acgcatagtc aatgttattt tcccacggtg aggcatatca ttttcggagc
    13021 ctccatctgt ttgaggattt cctgaaccat tacctacttg tgcaatggca ctttctacca
    13081 tgtagttttc gccgtcttcc atatatttat tggcgacagc atatactttt tgttcaattt
    13141 gtttgacaaa tgcattggtt tttttgatat ctgttccttc gggaaattca atataaacca
    13201 taatttgatt gggttggttt tccgggaaga aaagcacatt aggtttggct attccaaccg
    13261 ccacaaacga taaaatcaac aatagaaaag taccaaaaaa gaatactaac ggttttttac
    13321 cggttagggc atattgtaaa aagttgcggt atttggtttc catccacacc aaaataattc
    13381 gctggaaaaa gtcttgtgca cgaaccaaat agtatttgta aatccatagc atgattgcaa
    13441 aaaacaacat taagtttcct aaacctttta atccaccgga ttttgtatta aatcctgcaa
    13501 ctaacattac taagccaatg actcctacaa aaccacttaa tttgattaaa gcttttttag
    13561 ataattcttc ctctttcagt ttcataaaat cagctgtcaa catggcgttg ataatcaatg
    13621 ccacaaaaag tgaggaaaat aacacgactg acaatgtcat tgggaaataa atcatgaatt
    13681 ttcccatcgt tcctggccat aatcctaaag ggaaaaatgc agctaaagtt gttgctgtgg
    13741 atgcaataat tggccaagca atttcaccaa ttccttcaat ggcggcttgt tttcgagaca
    13801 tgccttggct catcaacgtg tacacgtttt caaccaccac aataccgttg tctacgagca
    13861 ttccgagccc cataaccaat ccgaaaagta ccatggtgtt taaagtgatg ccaaaagaag
    13921 ccaaaatcat gtaggacatg aacatggata atggaatggc aaatcctacg aaaatggcat
    13981 ttctaaatcc taagaaaaac atcaaaacgc caattactaa aatcacccca aagatgatgt
    14041 tgtttaccaa gtcatctact tgagcaatgg ttcgggttga ttgatcgtta gcatacgtta
    14101 tcgtcaaacc ttccggaaaa taattttctt tcgctttgtc aacaatagct tttaaccctt
    14161 caacagcctc caccatgttt ttaccgctac gttttttgat gtcgagcatn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna agtggtacgc tctttttctt tgaattcgat
    14341 agttgcaata tctctcagat aaacgtttcc atcctgtttt tttacaatga tgttttctaa
    14401 atcagaaggt ttgttgattt caccaatgac acgaatgttc tttttgatac cattggtaga
    14461 aacactaccg ccggaaaccg ttttattttc atatttcaca ctgctgataa tgtcgtcaaa
    14521 actaactttt gcagcggaca ttttgtacat atcaaccgca atttcaactt ctttatcttc
    14581 cgctccacga atggctgctt ctttaatttg aggaagtctt tcaatgtgtt cctctaaata
    14641 ttcagcatat tcttttagtt tttcagaggt aaaatcaccg gttagattga tgtttaaaat
    14701 aggcacttct tcggcaatgt ttaagtcgaa tacgttgggt tctacttttc caccgccatc
    14761 aattgttggc cattcggtat ccgatttaac tcggtcaact ttatctttta ccttttgttt
    14821 ggcatcttct acggatatat cttcgtcaaa ttccaccaaa atcattgaat aatcttccaa
    14881 tgtatttgaa gtgatttttg taactccgga tatgttgttg aattcttctt ccaaaggttt
    14941 ggtaatcagt ttttcaacat cttcagctga gtttccagga ttgatggaac ttacgtatat
    15001 tttggtttca atgatttcag gaaaactttc gcgaggcatc gaataatacg aaatcaaacc
    15061 gccaactaat aatatggctg taatcacata gaccgtcatt ttgttgttga tggcccatgt
    15121 tgatatggcg aaattcttgt gtatgttaag actcatgttt ctttttgttt atgagtttaa
    15181 agtttgatta cttctactgt ttgtccgtct tttaaggatt ttccaccttc ggttataaca
    15241 atttcacccg attgtagtcc tgattttact tccacaaatg cacccgaggt gtaaccaatt
    15301 tcaattgttt tttgagtagc catcgcattg tttttgctgt caactttatc aatcacataa
    15361 attactttac tgccatcagc cgtttcttga atgatgtttt caggaagtaa caatgctttt
    15421 tggtttttat aatcttcgat ttttaaaaca gctacttggt tgggacgtaa taaattatcc
    15481 ggatttggta aagcgatttc aataccaaac gttcgattgt ttggattgat atagtttcca
    15541 acttgacgca cttttccttt gtaattttta cctaaagaat ttaaatgcac gtctacaaca
    15601 gcacctactt ttaattgtcc gatgtagttt tcaggaacat tagccgtgac atacatgttg
    15661 tttaagttca caattctgaa taactccatt ccgggagcta ctacttttcc tttttcagtt
    15721 aatacctcat cgatagttcc gctgaaagga gcagtgacca ccgttttgtt caattgtgat
    15781 tttatttgag ccaccatttt ttgttggctt tccatactgg ttttagcttg taagtattga
    15841 atttcagaac caattttctg atcccaaaga tttttttgtt tttcaaaagt ggttctagct
    15901 aaagccaatt gcgtttcagc ttgagctagt tgcgaactca ttccgccgtc gtcaatgcga
    15961 gctaaaactt gaccttttga aacacgttga cccgctttca catttacttg agtcaaaata
    16021 cctgagtatt ccggactaat catgatgttt tctttggtgt caacatttcc ttgtatttca
    16081 atgtagtgcg taaaaagtgt atcttttaac gtaattgttt gtaccaaagg attttttttg
    16141 ttgggatcta attcagcaat tgctttgtca agttttgcta aatcggcaac cagtttttca
    16201 tattctttat gaacgctttc acgtgaagat ttgatttttg cgagatcttt tgattggatg
    16261 acttcgtcaa gatttccggt tttattttca cctgaacagg ctgctaataa aagcgtaagg
    16321 accgttatgg aaagtatttt tttcattggg aggt
//
LOCUS       DAOTCW010000048        15867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2109_length_15867_cov_29.291487, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000048 DAOTCW010000000
VERSION     DAOTCW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 15867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15867
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2109_length_15867_cov_29.291487"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="P5335_05625"
     CDS             complement(<1..190)
                     /locus_tag="P5335_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-pyrroline-5-carboxylate dehydrogenase"
                     /protein_id="HRZ74388.1"
                     /translation="MLKGFFHVPKAVNEPVKSYAPNSPEKAAVLAAYKEMWNTKIDVP
                     LYIGSEEIRTGNTRNMSAP"
     gene            complement(330..716)
                     /gene="apaG"
                     /locus_tag="P5335_05630"
     CDS             complement(330..716)
                     /gene="apaG"
                     /locus_tag="P5335_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRZ74389.1"
                     /translation="MVSQITRGIKISVSTSFEGTYFKNYKLHFAFSYHITIENHSKDS
                     VQLMSRHWEIFDSLNNVEVVDGEGVIGKKPVLKPGENHTYSSGCLLSSPFGAMKGHFN
                     MINFTTTKNFRVIVPTFKLSAPFALN"
     gene            complement(720..1817)
                     /locus_tag="P5335_05635"
     CDS             complement(720..1817)
                     /locus_tag="P5335_05635"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HRZ74390.1"
                     /translation="MSTKRLKDYRGISCLNCNHPLDISDKFCPNCGQKNTTKRLALRD
                     FIDEFLANFYAYDSKVKHTIVSLFTKPGKAAKEFITGKRLYYANPFRFYLSVSLIYFI
                     FSGLVEKFGEVENTDFTIGNEKVSINDSIAKYQEKENLQEKIDSSTVKRKKDRNNIFF
                     DETKISDTLYTEKQLEGKNIFKRTFLKIDTYTTFLEKSKEKDFDKILDTLKHENSNWN
                     KFLLKKSFQVNEFNSKDDSAVEKFANYLDEKLPFILFISLPFLTIVFAIIYFRSKLNY
                     AEHMVFVFSVMSFVFLLFFLAELINLLINIDLSSFIPIVLLFYLYKALRNFYQQSRLK
                     TILKFVLLTFMLPTTALFVAVIMVFIGFILY"
     gene            complement(1838..3091)
                     /locus_tag="P5335_05640"
     CDS             complement(1838..3091)
                     /locus_tag="P5335_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HRZ74391.1"
                     /translation="MIQIRLILIFSFISSFLFAQPEQETNPPFNIKTISFIQNNQNVM
                     PIFKLGEQFQFVFDDLYGDEANYYYTITHCDYDWTKSDLNLNEYLVGLDNQRIITYDN
                     SFNTLQMYSRYRIMFPNSITKGFKVSGNYIITILNNNQEVVFSRKFILYEELVNVPML
                     VKNPRDVRDLYSKHNLEFYVKPANIALQNPIQNVKIVLLKNDIWHTAIMNIKPMYTLG
                     TDLYYKYDKETQFWAGNEFLYFENKDIRNAANNVRRITLTGIYNTFLQTNFPRSTLKY
                     TYAPDANGIFVIQNIDARDDVNVESDYSWVFFSLSAPTFYEKKDIYVTGMFNNYALTS
                     ENKMDYNKEKGVYEKAIIIKQGFTNYEYTIADSNGKVDYEKAIDGNFIQTENNYTCLV
                     YYRGNNDRYDRIIGRGMTNSIYMTK"
     gene            3450..4799
                     /locus_tag="P5335_05645"
     CDS             3450..4799
                     /locus_tag="P5335_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HRZ74392.1"
                     /translation="MSTDIRIKKGLDLKLVGEADSKVSAVPRSQVYSINPSDFHKVTP
                     KLVLKEGTEVKAGETIFYSKNDEAVKFASPVSGKIVEIRRGDKRVILEIRIQADAQDI
                     FVDHGKKNPKEMTKEEVKAHLLASGCWPFIKQRPYDVIAHSNDTPKAIFVSGYSSAPL
                     SADVEVALKDKVASFQAGIDALTKLTSGKVHLSVNGKSDSILNRISGVELHKVFGPHP
                     AGNVGIQISKIDPINIGERVWVVAPQDVAIIGDLFLTGKFEANKVIALAGSEVANPQY
                     YSVRICAQMKEIVSGKLASDNVRIISGDVLTGDKVNADGNLGYYANSVTVIPEGNYYR
                     MFGWLPFSGIDKHSIQRTALSWLMPGKKYKADTNLNGEERAFVVTGEMEKLMPLDIYP
                     MQLLKACLANDIEKMENLGIYEVAPEDFALIDYADTSKIEAQVIIREGLDLMINEVG"
     gene            4809..5960
                     /locus_tag="P5335_05650"
     CDS             4809..5960
                     /locus_tag="P5335_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HRZ74393.1"
                     /translation="MKFLRDKIDQIKKPFGKGEKFEKFAPAVNSLDTFLFVPNHTTKR
                     GAHIRDAVDLKRTMITVVLALIPALIFGIWNAGYQNLSQLQEVVPFMDAFIHGLWKVL
                     PMIVVSYGVGLGIEFYFAIVRGHDVNEGYLVTGLLIPMIMPVDFPLWMLALSVAFAVI
                     IGKEAFGGTGMNILNPALTARAFAFFAYPTFMSGDKVWVSDATTMDAISGETILGKLA
                     AGKEAGYDAWSMFAGTIPGSISETSVLWILVGAAILVITGVGSWRIIVSGFLGAAFTA
                     FLFSLWGANPLMNFGMLHLLVGGLAFGIVFMATDPVSAAQTNRGKWIYGFLIGLFSIL
                     IRVFNPAYPEGVMLAILLLNVFAPTIDHYVVEGNIKRRKKRVAKQLKTA"
     gene            5963..6685
                     /gene="nqrC"
                     /locus_tag="P5335_05655"
     CDS             5963..6685
                     /gene="nqrC"
                     /locus_tag="P5335_05655"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HRZ74394.1"
                     /translation="MDRNSNGYTFMFAAVMVAIVGALLAFAATSLKDKQDENIRKEKM
                     QNILASVGVFVEREAAPDMFTTVIKEQLALKSDGTIDESSKAFNIDLKNELSKKEEDQ
                     RFPLYVAEQEGKKFYIVPLRGSGLWDAIWGYVALEEDLNTIKGTVFDHKGETPGLGAE
                     ITQKWFQDRFIGEKIADKSGEIVGVEVAKGNTDQSKEDNKVDAISGATITGNGVTAMI
                     KERLAHYKNYFSTQKSKLALNN"
     gene            6691..7365
                     /locus_tag="P5335_05660"
     CDS             6691..7365
                     /locus_tag="P5335_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HRZ74395.1"
                     /translation="MSEEKEALFSKKNRFLLSDPLNDNNPITVQVLGVCSALAITVQL
                     KPAIVMAISVTVVTAFSNVIISLLRNLIPNRIRIIVQLVVVAALVILVDQVLKAYSYD
                     VSKQLSVFVGLIITNCIVMGRLEAFALANKPWRSFLDGVGNGAGYGLLLVIVAFFREL
                     LGSGKLFGMEVLGHKGVTIAESTGLYAFGYENNGLMLLSPMALIVVGIIIWIQRSKNR
                     KLIEKH"
     gene            7395..8012
                     /gene="nqrE"
                     /locus_tag="P5335_05665"
     CDS             7395..8012
                     /gene="nqrE"
                     /locus_tag="P5335_05665"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HRZ74396.1"
                     /translation="MQELASLFVKSIFIENMIFAYFLGMCSYLAVSKTVKTAVGLGVA
                     VVFVQAITIPVNYLLDNYLLKPGALSWLGEEYAAYDLSFLSFIMFIAVIASMVQLVEM
                     IVEKFAPALYGALGIFLPLIAVNCAILGGSLFMQQKDFGTVTEAITYGVGSGLGWFLA
                     ILAIAAIREKIAYSHVPAPLRGLGITFLITGLMAIAFMSFMGIKI"
     gene            8029..9324
                     /gene="nqrF"
                     /locus_tag="P5335_05670"
     CDS             8029..9324
                     /gene="nqrF"
                     /locus_tag="P5335_05670"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HRZ74397.1"
                     /translation="MEYSVIIASVVVFLLVVFLLVAILLGAKAKLLPSGPVKIKINGE
                     TEIEVESGNTLLSTLGNSKIFLPSACGGGGTCIQCKCVVTSGGGSILPTEEPHFTRKE
                     IAHGWRLGCQVKVKNDMVIEVPEEVFGIKKFEAKVYSNYNVASFIKEFIVELPDDMHY
                     EAGGYIQIEIPECEVKYSDIDITAHPEEHPGEPEKFKAEWDKFNLWPLVMKNPELVER
                     AYSMASYPAEGRKIMLNVRIATPPWDRAKNGWAAVNPGVASSYIFSKKSGDPVTVSGP
                     YGEFFINESESEMLYVGGGAGMAPMRSHLYHLFRTVKTGRKVTYWYGGRSKRELFYVD
                     HFRALEKDFPNFKFYLALSEPMPEDNWKVKDGINGEGDGFVGFIHNCVIDNYLSKHDS
                     PEDIELYFCGPPMMNNAVQKMGEDFGMPPENIRFDDFGG"
     gene            9480..9845
                     /locus_tag="P5335_05675"
     CDS             9480..9845
                     /locus_tag="P5335_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit F"
                     /protein_id="HRZ74398.1"
                     /translation="MESLSKQELHNLAMNHVGKELESKGFEFIAVNSQLKRHPQFVCV
                     DKPTNTLHFVLVTVFNYPDNPNEYDEIWMETFKTHAFKLKAKVLYAGVGIANATDYEK
                     PVTKNEDFIINYNGIKEIS"
     gene            9823..10842
                     /locus_tag="P5335_05680"
     CDS             9823..10842
                     /locus_tag="P5335_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009416716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRZ74399.1"
                     /translation="MELKKLVRAIVFILLLISCQENQSEQVFLIQGEAQGSTYAIKYV
                     NSNEVVTKASIDSLLIAFDLSLSTYRPESIISKINSGDSTIVVDDFFVETFKTSQKIH
                     LETDGLFDPTIGVLVNAYGFGPVKEKIDLNPSKIDSLLTFVGFDKVKLNEDKTISKKH
                     PETYFDFNAIAQGFSVDVVTNFLKSKGIQNGIVEIGGEIFAFGKNTLEKKNWIVGIDN
                     PTQSPEKRTLIAKIKLENLGMATSGNYRKVKVDSLTGKKFVHIINPKTGLSEKSSILS
                     ATVLASKCIDADGFATAFMIMDLVETKKLLQKRTDLHVLLIYMDENNQIQQFKTANFE
                     KLIIN"
     gene            complement(10835..11605)
                     /locus_tag="P5335_05685"
     CDS             complement(10835..11605)
                     /locus_tag="P5335_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRZ74400.1"
                     /translation="MKRLFKFILNTIPRPILIRLSYLARPILAFLLKGNRFTDPIDGK
                     SFKMFLPYGYGKQRNNVLSPSTLSLERHRLLWLYLKDETDFFISPKKVLHFAPEQAFY
                     KLFRNQKNLDYTTTDLYSPLADVKADICNLPFEDNSYDIILCNHVLEHIPDDSKAMQE
                     LFRVLKTGGMGIFQIPQDLSRATTFSDDSITDEKERAKIFGQYDHVRVYGRDYFDKLR
                     SIGFEVLEEDYTSKISPELIEKYCLAKGEIIPVCFKTS"
     gene            complement(11602..12423)
                     /gene="map"
                     /locus_tag="P5335_05690"
     CDS             complement(11602..12423)
                     /gene="map"
                     /locus_tag="P5335_05690"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRZ74401.1"
                     /translation="MIIPKTREEIELMRESALLVSKTLGEIAKQIKPGVTTLYLDKLA
                     EQYIRDNGGIPGFLGLYGFPNSLCMSPNAQVVHGIPNNKPLEEGDVISVDCGVLKNEF
                     YGDHAYSFEIGEVAPEVKKLLKVTKESLYVGIRQFKAGNRVEDVGNAIQTYCESHGYG
                     VVRELVGHGLGRKMHEDPEMPNYGKRGRGKLFVEGMVVAIEPMINMGTKNIKQLKDGW
                     TILTADMKPSAHFEHNVALVDGKPELLSTFKYIYEALGIVSQEEDEFRKNPSVIK"
     gene            12592..13878
                     /locus_tag="P5335_05695"
     CDS             12592..13878
                     /locus_tag="P5335_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRZ74402.1"
                     /translation="MSKYVSAQEAVKAVKSGDRVYIQAAAATPTVLTKALAERASELK
                     NVEICHLHTEGEAPYANPELAESFHVNSFFIGANVRHTLKAGNGSYTPVFLSELPMLF
                     RKKVLPLDVVFIHVSPPDRHGYCSLGVSVEASLAAVENAKIVVAQVNPNMPRTFGDGI
                     LHESEIDYLVEVNVPIFSHGIEAFTPEEEKIGEYIASLIEDKSTLQMGIGSIPNAALS
                     KLYNHKDLGLHTEMFSDGVIDLIEKDIINCNYKGTTRGRVLATFLMGSRRLYDFVDDN
                     SFIEMKESSAVNDTARIRKNPKMVAINSAIEVDLTGQVCADSIGGRMYSGVGGQMDFI
                     RGASLSVGGKAIIALPSVTKRGESRIVPFLKQGAGVVTTRSHVQYIITEYGIANLYGK
                     TLKQRVSEMVKIAHPNHREWIEKAYYELMEIKPLVC"
     gene            complement(13927..15327)
                     /locus_tag="P5335_05700"
     CDS             complement(13927..15327)
                     /locus_tag="P5335_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRZ74403.1"
                     /translation="MRTIIASFLLFSVISWSQNKASDSILTLDAYLGYVKQFHPLVKQ
                     ANIEVSQAQAGIIASRGGFDPKIEVDYENKQFKSTEYYDLLNTTFKIPTWYGVEIKAG
                     FDRMEGVYLNPQNTTPDNGLAILGISVPLGQGLLINNRMADLKKAKLYAKLSQAERDL
                     EVANVLYLASNSYFHWYKNYNEYKLYETFLKNAKVRYDGITSLISAGDRPAIDSVEAG
                     ILVKSRQLSLEQSRLKLVKSKLELSNYLWFDNNLPLELQDFITPEATLEKTVENTLKT
                     NGLLLANIDFENHPKIKSLQNKVSILEVDKRLKGNLLLPTINLNYNYISEPEFVEDFN
                     TLNYKYGVNFSFPLFLRKERGNFKIAKFKLQDAELDLQFENQVLKNKIEYQLEEISSL
                     KNQINIANELVANFKTMLDSEEKLFLFGESSLFIINSRENSLLNTSLQQIDTQVKFCV
                     SNSDLFKLIANPEFNN"
     gene            complement(15335..>15867)
                     /locus_tag="P5335_05705"
     CDS             complement(15335..>15867)
                     /locus_tag="P5335_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRZ74404.1"
                     /translation="VNKLQNQVTNYKMRNELYYIKAPQNGYVNRALQSGLGETIKEGT
                     PVVSIMPSKYDIAVETYIDPIDLPLIHKGEKVRIWFDGWPTIVFSGWPNTSYGTFGGS
                     IVAVENFISTNGKYRILIAQDPTDKPWPKQLSIGAGAQTFALLDNVPIWYELWRKLNG
                     FPPDFYTPKQTNVSKK"
BASE COUNT         5262 a         2377 c         2786 g         5442 t
ORIGIN      
        1 gtggagcact catgttccga gtgttaccgg tacgaatttc ttcactacct atgtataatg
       61 gaacatcaat tttagtattc cacatttctt tataagctgc taacacagct gccttttcag
      121 gactatttgg agcgtatgat ttaacgggtt cattgaccgc ttttggaaca tgaaaaaaac
      181 cttttagcat gataaagtgt gtttaaaatt aaacaaattg ataataaaaa cttgtaattc
      241 ttaagaatta taaattagaa tacaaaagta caaaggtttt tttgaatttt tgataacaaa
      301 agttacaaag caaaatggga atagttgaat taatttaaag caaaaggagc actcaattta
      361 aaagtgggta caataaccct aaaattttta gtggtagtaa aattgatcat attaaaatgt
      421 cctttcatcg ctccgaaggg tgaagagagc aaacagccgg aactataagt atgattttca
      481 ccgggtttca acactggttt tttaccgata actccttctc catcaacaac ctctacattg
      541 tttaaagaat caaaaatttc ccaatggcga gacatgagtt gtacagaatc tttactatga
      601 ttttcaatgg taatgtgata actaaaagca aaatgaagtt tatagttctt gaagtaggtg
      661 ccttcaaaac tagttgaaac tgaaattttt atgcctcttg ttatttgtga aaccataaat
      721 taatataaaa taaaaccaat aaaaaccata attactgcca caaaaagtgc agtagttggc
      781 aacataaagg tcaaaagtac aaattttaaa attgttttca aacggctttg ttgataaaaa
      841 tttcttaaag ctttatacaa ataaaataat aatacaatag ggatgaagga tgataagtcg
      901 atattaatca gcaaattaat caattcagca agaaaaaata ataaaaaaac aaaactcatg
      961 acgctaaaaa caaaaaccat gtgttctgca taattcagtt tgcttctaaa ataaataata
     1021 gcaaagacaa tggttagaaa tggtagcgaa atgaaaagga taaaaggcag tttttcatct
     1081 aaatagtttg cgaatttttc aaccgctgaa tcgtctttgc tattaaattc gttgacttga
     1141 aaactttttt tcaataaaaa tttattccaa ttgctgtttt cgtgtttcaa tgtatccaat
     1201 attttatcaa aatctttttc ttttgatttt tctaaaaaag tggtataagt gtctatcttt
     1261 agaaaagttc ttttaaaaat atttttacct tcaagttgtt tttcggtgta taaagtgtcg
     1321 cttatttttg tttcatcaaa gaatatgttg tttctgtctt ttttcctttt taccgttgat
     1381 gaatctattt tttcttgtaa gttttccttt tcttggtatt ttgcaatact gtcatttatg
     1441 ctcacttttt catttcctat ggtaaaatcg gtgttttcaa cttcaccgaa tttttcgacc
     1501 aatccactaa aaataaaata aattaaagag acacttaaat aaaatcgaaa aggatttgca
     1561 taataaagtc ttttgcctgt aataaattct ttcgctgctt ttccgggttt tgtaaataaa
     1621 gaaacaatag tgtgttttac tttagaatca taggcataaa aattggctaa aaattcatca
     1681 ataaaatccc gcaatgctaa tcgtttggtt gtgttttttt gcccacaatt tggacaaaat
     1741 ttatctgaaa tatccaatgg atgattgcaa ttcaggcaag aaataccacg gtagtcttta
     1801 agtctctttg ttgacacgtt taatctttat ttgttgttta ttttgtcatg tagatagaat
     1861 tggtcattcc tctgccaata attcgatcat atcggtcatt atttcctcta taataaacca
     1921 agcaagtata attgttttct gtttgaataa aattaccatc aattgccttt tcataatcta
     1981 ctttcccatt tgaatcggcg atagtgtatt cgtaattggt aaatccttgt ttgatgatga
     2041 tggctttttc gtaaactcct ttttctttat tataatccat cttattttca gaagttaagg
     2101 cgtagttgtt gaacattccg gttacataaa tgtctttttt ttcataaaaa gttggagcac
     2161 ttagtgaaaa aaatacccaa gaataatcag actctacatt tacatcatcc cgtgcatcta
     2221 tattttgaat aacaaaaata ccattagcat cgggtgcata ggtatatttt aaagtggacc
     2281 ggggaaaatt ggtttgtaaa aaagtgttat aaattccggt aagggttatt cttctgacat
     2341 tgttggcagc atttcgaatg tctttatttt caaaatataa aaactcatta cctgcccaaa
     2401 actgtgtttc tttatcatat ttataataca aatcagtccc tagtgtatac attggtttta
     2461 tattcataat ggccgtgtgc caaatgtcgt ttttcaacaa tacaattttt acattttgaa
     2521 tagggttttg caaggcaata ttagcgggtt ttacataaaa ttccagattg tgtttagaat
     2581 ataaatccct aacgtcacgt ggatttttaa ccaacatagg tacgttaact aattcttcgt
     2641 ataatatgaa ttttctggaa aaaacaactt cttgattgtt gtttaatata gtaatgatgt
     2701 aatttccact aaccttaaag ccttttgtaa tactattagg gaacataatt cggtaccttg
     2761 aatacatttg taaggtgtta aatgaattat catatgttat tattcgttgg ttatctaatc
     2821 caactaggta ttcatttaaa tttaaatcag atttagtcca gtcataatca caatgggtta
     2881 tggtgtaata ataattggct tcatctccat ataaatcatc aaaaacaaat tgaaattgtt
     2941 cgcctaattt aaaaatgggc ataacatttt gattattttg gataaaagaa atggttttaa
     3001 tgttgaaagg ggggttagtt tcttgttcgg gctgagcgaa caaaaacgat gaaataaaac
     3061 taaaaataag tataagacga atttgaatca tattgtagat tgttaaaaac aaaaaagtaa
     3121 atataatatt atttgtttac ataaaagatg ccaaatttaa ttttggcaat tggtattttt
     3181 aagtgtcatt ttaaattcga gaatttagaa gatatatata tgataatttg atcgaatttc
     3241 aaaaagaatt actttttgaa ttatatttat tgcttctatc ttaatcaata atgaaagtga
     3301 gaatagaagc ttattattta gaattaatat aaataaataa aaatcgatta gacatttgtt
     3361 aattcatatg tagctattac tatttaattt ttaaatttgc tccgttttca attaagttaa
     3421 aacaaatact aatcatatct tcataacata tgtctacaga cattcgaatt aagaaaggat
     3481 tagacttgaa attagttgga gaggcagatt cgaaagtttc tgcagttccc cgttctcaag
     3541 tttactccat aaatccttct gactttcata aagttacacc aaaattggtg cttaaagagg
     3601 gaaccgaagt aaaagctggt gaaaccattt tttattctaa aaatgacgag gctgtaaaat
     3661 ttgcatctcc tgtaagtgga aaaattgttg aaatcagacg cggtgataaa cgagttattt
     3721 tggaaatcag aattcaagct gatgcccaag atatttttgt tgatcatgga aagaaaaatc
     3781 ctaaagaaat gaccaaagaa gaagtcaaag cacatttgct tgcttctggt tgttggccgt
     3841 ttatcaagca acgcccttat gatgtcatag ctcattcaaa tgatactcct aaagcgattt
     3901 ttgtttctgg atatagttct gcaccacttt cagcagatgt tgaagtagct ttaaaagata
     3961 aagtagcttc attccaagcg ggtatagatg ctttaacaaa attaacttcc ggtaaagttc
     4021 atctttctgt aaatggaaaa tctgattcaa ttttaaatag aatttcaggt gttgaattac
     4081 ataaagtatt tgggcctcat cctgcgggta atgttggcat tcagattagt aaaattgatc
     4141 caataaatat cggcgaaaga gtttgggtgg ttgctccaca agatgtagca atcattggag
     4201 acttattctt aaccggtaaa tttgaagcaa ataaagttat agctttagca ggttctgaag
     4261 ttgcaaatcc acaatattat tcagttagaa tctgtgctca aatgaaagaa attgtttccg
     4321 gtaaattagc ctctgacaat gttcgaatta tttctggcga tgttttaacg ggtgataaag
     4381 ttaatgctga tggaaattta ggttattatg caaattcagt tacagttatt ccagaaggaa
     4441 attactatcg aatgtttggt tggttgccgt tttcaggtat agataaacat agtattcaac
     4501 gtacggcttt atcttggtta atgccaggga aaaaatataa agctgatacg aatttaaatg
     4561 gcgaagaacg ggcttttgta gtaacgggag aaatggaaaa attaatgcct ttagatattt
     4621 atccaatgca attattaaaa gcgtgtttag caaacgatat tgaaaagatg gaaaatttag
     4681 gaatatacga agtagctcct gaagatttcg ccttgataga ttatgctgat acttctaaaa
     4741 ttgaagctca ggttattatt agagaaggtt tagatttaat gattaacgaa gtaggttaaa
     4801 atagaattat gaagttttta agagataaaa tagaccaaat taaaaagcct tttggtaaag
     4861 gcgaaaaatt tgaaaaattc gcaccagctg tgaattcatt agatactttt ttattcgtgc
     4921 ctaatcacac gacaaaacgc ggagctcata ttagagatgc ggtagattta aaacgaacca
     4981 tgattacagt ggtattggct ttaataccgg ctttaatctt tggtatttgg aatgccggat
     5041 atcaaaattt atcacaatta caagaagtag taccattcat ggatgcattc atacatggac
     5101 tatggaaagt actgcctatg atagtagttt cttacggtgt tggattgggt attgaattct
     5161 atttcgccat tgtaagaggg catgatgtaa acgaaggtta tttagtaacc ggtttgttaa
     5221 ttcctatgat tatgccggtt gattttcctt tatggatgtt agctttatcg gttgcttttg
     5281 ctgtaattat cgggaaagaa gcttttggag gaacaggaat gaatatttta aatccagctt
     5341 taacagcaag agcatttgct ttttttgctt atccaacatt catgtcaggt gataaagttt
     5401 gggtttctga tgcaacaaca atggatgcta tttcaggaga gactatttta ggaaaattag
     5461 ctgccggaaa agaagcaggt tatgatgctt ggagtatgtt tgccggaaca attccgggtt
     5521 ctatttcaga aacatcggtg ctttggatct tagtaggtgc tgctatttta gttataactg
     5581 gagtaggtag ttggagaatt atagttagtg gattcttagg agccgctttt acagctttct
     5641 tgttctcttt gtggggagca aatcctttaa tgaattttgg aatgttacat ttattagttg
     5701 gtggtttagc attcggaatt gtatttatgg caaccgaccc tgtatctgca gctcaaacca
     5761 atagaggaaa atggatatat ggattcttaa taggtttatt cagtatatta attagagtat
     5821 ttaatccagc ctatccggaa ggggtaatgc tagcaatatt attattgaat gtttttgcac
     5881 ctacaatcga ccattatgtg gtagaaggaa atatcaaaag aagaaagaaa agagttgcta
     5941 aacaattaaa aacagcctaa tcatggatag aaatagtaac ggatatacgt ttatgtttgc
     6001 tgcggtgatg gtagcaatag taggagcttt acttgctttt gctgcaacat ctttaaaaga
     6061 caaacaagat gaaaacataa gaaaagaaaa aatgcaaaat attttggctt ctgttggagt
     6121 ttttgttgaa agagaagcag caccagatat gtttacaact gttattaaag aacaattagc
     6181 tttgaaatca gatggaacaa ttgatgagtc ttccaaagct tttaatattg atttaaaaaa
     6241 tgagttgtca aaaaaagaag aagaccaacg ctttccttta tatgttgctg aacaagaggg
     6301 aaaaaaattc tacattgttc ctttaagagg ttcaggtctg tgggatgcta tctggggtta
     6361 tgtagcttta gaagaagatt tgaataccat taaaggtact gtatttgacc ataaaggtga
     6421 aactcccgga ttaggggcgg agattactca aaaatggttc caagatcgtt ttattggtga
     6481 aaaaattgcc gataaatcag gggaaattgt tggtgttgaa gtagctaaag gaaatacgga
     6541 tcaatcaaaa gaagataata aagttgatgc tatatcagga gctaccatta caggaaatgg
     6601 agttacagcc atgattaaag agagattagc tcattacaag aattacttct ctactcaaaa
     6661 atctaaatta gctttaaata actagaaatt atgtcagaag aaaaagaagc tttgttttca
     6721 aagaaaaata gatttttgtt gtctgatcct ttaaatgaca acaatccaat tacagttcaa
     6781 gtactaggtg tttgttctgc tttagcaatt acggttcaat taaaaccagc cattgtaatg
     6841 gcaatttcag ttaccgttgt taccgcattc agtaatgtta taatatcact attacgaaat
     6901 ttaattccta atcgtattcg tattatcgtt cagttggttg ttgtagctgc tttagttatt
     6961 ttagttgacc aagttttaaa agcctattct tatgatgtga gtaaacaact ttcagtattc
     7021 gtaggtttaa ttattaccaa ctgtattgta atgggtcgtt tagaagcctt cgccttagca
     7081 aataaacctt ggcgttcatt tttagacggt gttggtaatg gtgcaggata tggattatta
     7141 ttagttattg ttgctttttt tagagaactt ctgggttctg gtaaattatt cggaatggaa
     7201 gtattaggtc ataaaggggt aacaattgct gaatctaccg gattatatgc ttttggttat
     7261 gaaaataatg gattaatgtt attatctcct atggctttaa ttgtggtcgg tattatcatt
     7321 tggattcaac gttcaaaaaa ccgtaaatta attgaaaaac actaattata gcttagctat
     7381 taaactatta taaaatgcaa gaattagcaa gtttatttgt aaaaagtatt ttcattgaaa
     7441 atatgatttt cgcctacttt ttaggaatgt gttcctattt agcggtgtcc aaaacggtaa
     7501 aaactgccgt aggattaggt gtagcagtag tatttgtaca agcgataact ataccggtta
     7561 actatttgtt agataattat ttactaaaac ctggagcact ttcttggtta ggagaagaat
     7621 atgcagcata tgacttaagt tttttaagtt tcattatgtt tatagcagtt attgcttcta
     7681 tggttcaatt ggttgaaatg attgttgaaa agtttgcacc ggccttatat ggtgctttgg
     7741 gaatttttct tccgttaata gctgtgaatt gtgcaattct tggaggttca ttatttatgc
     7801 aacaaaaaga ttttggaact gtaactgaag ccatcactta cggagtgggg tcaggattag
     7861 gatggttttt agccatttta gccattgcag ccataagaga aaaaatagcc tactcacatg
     7921 tgccagctcc tttgcgtgga ttaggaatta ctttcctaat aactggttta atggccattg
     7981 cgtttatgag ttttatggga attaaaatat aatttataca tactaagaat ggaatattca
     8041 gtaataatag ccagtgtagt cgtttttctt ttagttgttt ttctattagt agctattctt
     8101 ttaggagcaa aagctaaact tttgccttct ggtccggtaa aaattaaaat caacggcgaa
     8161 accgaaatag aagttgagtc tggaaatacc ttactttcaa cactaggaaa tagtaaaatt
     8221 ttccttcctt cagcttgtgg aggtggagga acttgtattc aatgtaaatg tgtagttacc
     8281 agtggaggtg gatcaattct accaactgaa gaaccgcact ttaccagaaa agaaatagct
     8341 catggttgga gactcggttg ccaagttaaa gttaaaaatg acatggttat tgaagtgcca
     8401 gaagaggttt ttggaatcaa aaaattcgaa gcaaaggttt attcaaatta caatgtagca
     8461 tcttttatta aagaatttat tgtagaattg ccggacgata tgcattatga agccggaggt
     8521 tatatacaaa tagaaattcc tgaatgtgaa gtaaaatatt cagatattga catcacggca
     8581 catcctgaag agcatcccgg tgaaccggaa aaatttaaag cagaatggga taaatttaat
     8641 ttgtggccat tagtgatgaa aaatcctgaa ttagtagaaa gagcttattc catggcttct
     8701 tatcctgcag aaggaagaaa aatcatgttg aatgttcgta ttgctacacc accttgggat
     8761 agagctaaaa atggatgggc tgccgttaat cccggtgtcg cttcatcata tattttctca
     8821 aaaaaatcag gagatcctgt aacggtttcc ggaccttacg gtgaattttt catcaacgaa
     8881 agtgaatcag aaatgcttta tgtaggtggt ggagccggta tggctcctat gcgttcacat
     8941 ttataccacc ttttcagaac tgtgaaaaca ggtagaaaag taacttattg gtatggtggt
     9001 cgttcaaaac gtgaattatt ttatgtagat cactttagag cattagaaaa agattttcct
     9061 aattttaaat tctatttggc tttatcagaa ccaatgccgg aagataattg gaaagtgaaa
     9121 gatggtatta atggagaagg cgatggattt gttgggttta ttcataattg tgtaattgat
     9181 aattatttgt caaaacacga cagtcctgaa gatattgaat tgtatttctg tggtccgcca
     9241 atgatgaaca atgctgttca aaaaatgggt gaggattttg gaatgccacc ggagaatatt
     9301 cgttttgacg actttggagg atagtatttt ataaatcgct ttaaggttaa tttattttaa
     9361 accattaaga aattaagtat aatatttaat ttcttaatgg tttattttta ctataaattt
     9421 ctaatccttt tttgatttaa ttaagagttg tttttcccat ttctgcttac ttttgtttta
     9481 tggaaagttt atcgaaacaa gaacttcaca atctggctat gaatcatgtt gggaaagaac
     9541 tcgaaagtaa aggatttgag ttcattgcag tgaatagtca gttaaaaaga catccacaat
     9601 ttgtatgtgt ggataaacca actaatacac tccattttgt attggttact gtctttaatt
     9661 accccgataa tccaaatgaa tacgatgaaa tttggatgga aacgtttaaa acacatgcct
     9721 tcaaattaaa agctaaagtc ctttatgcgg gagttggaat agctaatgct accgactatg
     9781 aaaaaccggt tacaaaaaat gaagatttta tcatcaatta taatggaatt aaagaaatta
     9841 gttagagcaa tagtttttat tttactttta ataagttgtc aagaaaatca aagtgaacaa
     9901 gtttttttga tccaaggtga agcacaagga agtacttatg caattaagta tgttaattca
     9961 aatgaagtag ttactaaagc ttcaatagat tctcttttaa tagcttttga tttgtcactt
    10021 tcaacgtata gacctgaatc aataatttca aaaatcaatt cgggtgattc taccattgtg
    10081 gtagatgatt tttttgtaga aacattcaag acatctcaaa aaatacatct agaaaccgac
    10141 ggacttttcg accctacaat aggtgttttg gttaatgcct atggatttgg accggttaaa
    10201 gaaaaaatag atttaaaccc atctaaaatt gatagtttgc tgacatttgt gggttttgat
    10261 aaagtaaaat tgaatgaaga caaaacaatt tctaaaaaac atcctgaaac ttattttgat
    10321 tttaatgcta ttgctcaagg attttctgtg gatgttgtta ccaattttct aaaatcaaaa
    10381 ggcattcaaa atggtattgt tgaaatcgga ggtgaaattt ttgcatttgg taaaaatact
    10441 cttgaaaaaa agaattggat tgttggaatt gataatccta cacaatctcc ggaaaaacgt
    10501 acattaattg ctaaaattaa attggaaaat ttaggaatgg ctacttccgg gaactatcga
    10561 aaagtaaaag tagatagtct taccggtaaa aaatttgttc acattatcaa tccaaaaacc
    10621 ggactttcag aaaaaagctc gattttaagt gcaaccgttc tagcctcaaa atgtatagat
    10681 gcagacggtt tcgctactgc atttatgata atggatttag ttgaaactaa aaaactcctt
    10741 caaaaaagaa cagatttgca tgttttgttg atttatatgg atgaaaataa tcaaatacaa
    10801 cagtttaaaa cagctaattt tgaaaaactg attattaact agttttaaaa caaaccggga
    10861 taatttctcc tttagccaaa caatattttt ctattaattc aggagaaatt tttgaagtat
    10921 aatcttcttc aagaacctca aaaccgatac ttctcaattt gtcaaaataa tctcttccat
    10981 aaacccgaac atggtcatat tgaccaaata tttttgctcg ttccttttca tccgtaatcg
    11041 aatcgtccga aaaagtagta gctcttgata aatcctgagg aatttgaaaa atacccatgc
    11101 cgccagtttt taagacccga aacaattcct gcattgcttt tgaatcatcc ggaatatgct
    11161 ctaaaacgtg gttacacaaa ataatatcat acgaattgtc ttcaaaaggg agattacaaa
    11221 tatctgcttt cacatctgct aaaggggaat ataaatcagt ggttgtgtaa tctaaatttt
    11281 tttgatttct aaaaagctta tagaatgctt gttcaggagc aaaatggaga actttttttg
    11341 gagaaataaa aaaatccgtt tcatctttta aatacaacca taataatcga tgtctttcca
    11401 atgaaagcgt gcttggagat aacacattat tacgctgctt tccatagccg taaggcaaaa
    11461 acattttgaa actttttcca tcaattgggt cggtaaatcg atttcctttc aataaaaaag
    11521 ctaaaattgg tcgagctaga taactcaacc gaattaatat cggacggggg atagtgttta
    11581 aaataaattt gaataatctt ttcatttaat aaccgaagga ttttttctaa attcatcttc
    11641 ctcttgactt actattccta aagcttcata aatatattta aaagtagaaa gcaattccgg
    11701 ttttccatca accaatgcaa cgttatgttc aaaatgtgca cttggtttca tatccgcagt
    11761 caatattgtc cagccatctt ttaactgctt aatatttttt gttcccatgt ttatcatcgg
    11821 ttcaatagct acaaccattc cttctacaaa taactttccg cggcctcttt tgccataatt
    11881 aggcatttcc ggatcttcat gcatttttct gcctaatcca tgaccaacta attctctcac
    11941 aactccataa ccatgcgatt cacaataggt ttgaatagca tttcctacgt cttccactcg
    12001 attacctgct ttaaattgac ggatgccaac ataaagagat tctttagtaa cttttaataa
    12061 tttttttact tccggtgcca cttcgcctat ttcaaacgaa taagcatggt caccgtaaaa
    12121 ttcatttttt aaaacaccac aatctaccga aataacatca ccttcttcta atggtttatt
    12181 gtttgggata ccatgaacta cttgggcatt tgggctcatg caaagtgaat ttggaaatcc
    12241 gtataatcct aaaaaaccag gtataccacc attgtctcgg atgtattgtt cggctaattt
    12301 atccagatat aatgtagtaa caccgggttt gatttgtttc gcaatttcac ctaatgtctt
    12361 agaaaccagc aatgcacttt cacgcattaa ttcaatttct tctcttgttt ttggaataat
    12421 cattttcaaa aattaaaggc acaaaggtac acttttttgt tgtttttatt attttgttta
    12481 gtaggtttac ttttggtacg aacacctttt ctaaccaaaa aaacaaaaac aatgagaaat
    12541 atcatctttg atgcattaat ttgattttat ctttgtctaa aatttaatat catgagtaaa
    12601 tatgtatccg cacaggaagc tgtcaaagct gtaaaatcag gagatagagt ttatatacaa
    12661 gcagctgctg caaccccaac tgttttgaca aaagctttgg cagaaagagc cagtgaatta
    12721 aaaaatgtag aaatatgtca ccttcatacc gaaggagaag ctccgtatgc aaaccctgaa
    12781 ttagctgaaa gttttcatgt taattctttt tttattggtg ctaatgttcg tcacacatta
    12841 aaagccggaa atggctccta tacgcctgtt tttttgagtg aattaccaat gttgtttcga
    12901 aaaaaagtat tgccattaga tgttgttttt attcatgttt caccacctga taggcatggc
    12961 tattgttctt taggggtttc tgtggaagca tcattggcgg ctgttgaaaa tgctaaaatt
    13021 gttgtggctc aggttaatcc aaatatgcct agaacctttg gggatggtat tttgcacgaa
    13081 tcagaaattg attatttagt tgaggtaaat gtgcctattt tttctcatgg gattgaagcc
    13141 tttactcctg aggaagaaaa aattggagaa tatattgctt cacttattga agacaaaagc
    13201 actttacaaa tggggattgg gtctattcca aatgcagctt taagtaagtt gtataatcac
    13261 aaagatttag gtttacatac cgaaatgttt tcagacggag tgatagattt aattgaaaaa
    13321 gatataatca actgtaatta taaaggaact acacgcggac gagttttagc cacatttttg
    13381 atgggttcca gacgtttata tgattttgta gatgacaatt catttatcga aatgaaagaa
    13441 tcatcagctg taaatgatac agcccgaatt cgaaaaaacc caaaaatggt agccattaat
    13501 tctgctatag aagttgattt gacaggtcag gtgtgtgcag actcaattgg tggcagaatg
    13561 tattccggtg ttggcggaca aatggatttt atacgtggag cttccttgag tgttggcgga
    13621 aaggccatca ttgctttacc gtctgttact aaaagaggag aaagcagaat agttcctttt
    13681 ttaaaacaag gagccggtgt agtaacaacc cgttcacatg tacaatacat aattactgaa
    13741 tatggaattg ctaatcttta tggtaaaaca ttaaagcaaa gagtttcaga aatggtcaaa
    13801 atagctcatc caaatcatcg cgaatggata gaaaaagcct attatgaact gatggaaatc
    13861 aaaccattag tttgctaata aagagtattt aactgctttg tttttaatgg aaaacaaagc
    13921 agttttttaa ttattaaatt ccggattagc aatcaattta aataaatcgg agtttgagac
    13981 acaaaatttt acttgtgtat caatttgttg caaactagtg ttgagtagac tgttttcacg
    14041 agaattgatt ataaacaaag aactttctcc aaataaaaat aatttttctt cagaatctaa
    14101 cattgtttta aaatttgcta ctaactcatt tgcaatgttt atttgatttt ttaacgaact
    14161 aatttcttcc aattggtatt cgattttatt cttcaaaacc tgattttcaa actgtaaatc
    14221 caattcagca tcctgcaatt tgaatttcgc tattttaaag ttacctcttt cttttcttag
    14281 aaataaaggg aagctaaaat ttacaccata tttataattc aacgtattaa aatcttcaac
    14341 aaattcaggc tccgatatgt aattgtaatt taaattaatg gttggtaata acagattccc
    14401 tttgagccgt ttgtctactt ctagtatgct gactttattt tgaagtgatt ttatttttgg
    14461 atgattttca aaatcaatgt tagcaagcaa taatccatta gtttttaacg tattttcaac
    14521 tgttttttcc aacgtagctt caggagtaat aaaatcttgc aattctaacg gtaaattatt
    14581 gtcaaaccac aaataattcg aaagttctaa tttcgattta actaatttta atcgagattg
    14641 ttctaaactt aattgtctgg attttaccaa aattcctgct tcaacactgt caattgccgg
    14701 tcggtcaccg gcagatatta aacttgtaat accatcatat cttacttttg cattttttaa
    14761 aaaagtttca tatagcttgt attcattata gtttttgtac caatggaaat aactattaga
    14821 ggctaaatag agaacattag ctacttccaa atcacgttct gcttgcgata attttgcata
    14881 taattttgcc ttttttaagt cggccattcg attattaata agtaagcctt gacctaaagg
    14941 aaccgaaatt ccaagtattg ctaatccgtt atcgggagta gtgttttgag gattcaaata
    15001 cacaccctcc attcgatcaa aacctgcttt tatttcaacg ccataccaag ttggaatttt
    15061 gaaggtggta ttcaataaat cataatattc agttgattta aattgtttgt tttcgtaatc
    15121 cacttcaatt tttgggtcaa atccacctcg acttgctata attcctgctt gagcttggct
    15181 cacttcaata ttagcttgtt tcaccaaggg atggaattgt ttgacatagc ctaaataggc
    15241 atctaaggtt aaaatactat cagaagcttt gttttgagac caagaaataa ccgaaaaaag
    15301 caaaaaggag gcaattatag ttctcatgat ttccttattt ttttgataca ttggtttgtt
    15361 taggggtgta aaaatctggt ggaaaaccat ttaatttccg ccacagttca taccaaatcg
    15421 gcacattgtc caataaagcg aaagtttgag ctcccgcccc aatgcttagt tgcttaggcc
    15481 aaggtttgtc tgttggatct tgagcaatca aaatgcggta ttttccgttg gtgctgataa
    15541 aattttcaac agcaacaatg gaacctccaa atgttccgta ggaagtattt ggccatccac
    15601 taaaaacaat ggttggccat ccgtcaaacc aaattctgac tttttctcct ttatgaatga
    15661 gtggtaaatc tattggatca atatatgttt ccacagcaat atcatattta gatggcataa
    15721 tgcttacaac cggagttcct tctttaatcg tttctccaag accggactgc aacgcacggt
    15781 ttacataacc attttgtgga gcttttatgt agtaaagttc gttacgcatc ttgtaattag
    15841 taacctgatt ttgcaattta ttcactt
//
LOCUS       DAOTCW010000049        15582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2183_length_15582_cov_37.870484, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000049 DAOTCW010000000
VERSION     DAOTCW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2183_length_15582_cov_37.870484"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="P5335_05710"
     CDS             complement(<1..194)
                     /locus_tag="P5335_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRZ74405.1"
                     /translation="MAFDKEKVLQKCNEWSKNTLMETLEIEFIDAGEGFLKAKMPVNP
                     NVHQPMGLLHGGASVALAESV"
     gene            331..1896
                     /locus_tag="P5335_05715"
     CDS             331..1896
                     /locus_tag="P5335_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74406.1"
                     /translation="MRNSLFLLFIVFAISITSCRNDFEFEPSTGGLEFSRDTIYMDTV
                     FTNVSSSTYTLKVYNRSNKDISIPQIKLGKGLDSKYRMTVDGMVGNNRIFENVEMLAK
                     DSMYIFIETTVDIAQANPSDFLYTDQIEFGSGSTLQKVELVTLIQDAYFLYPQRFDDG
                     TTETLPIGDEEVYGFYLDENDPTNGNEYVWNNSKPYVIYGYAAVPANKTLTVQAGAQI
                     HFHADSGLIIGENGSIKVNGLPSNNPENLLENHVVFEGDRLEPLYDDVPGQWGTIWLT
                     QGSKNNEFTNCLIKNGTVGILVTGNTNLANPEADVKLKNVQIYDHSNVGVLARTGYIS
                     GENVVINSAGQAALACSYGGKYNFNHSTFNNNWPSSKQVSVLLNNYIEGAVPEIQPME
                     EATFTNCIIYGSNQIQMIIDKKESPIFNYKFDHCLIKFNNVNNVFTNNPLYQFTTDTE
                     HYTNCLIATNSAVFDPDFFDINSNQLIIGADSAAIGQANFSFSSGTFDILGKPRISPA
                     DLGAYNFVVFEED"
     assembly_gap    1897..1996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2098..2739
                     /locus_tag="P5335_05720"
     CDS             2098..2739
                     /locus_tag="P5335_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HRZ74407.1"
                     /translation="MKIKFLFIIAIISLVSCGKKEETKPETTTAKDTTTVEEIAVTTP
                     DSTTIAKAWEDYMTPAEQHKMLALENGIWNEEMTMWMEPGAEPMKNTMTAVSKMIYGD
                     RYQETVHKGDFMGMPFEGKSTLAFNKASQEYTSTWIDNMSTGIMVISGKYDEATKTIN
                     FSGTTVDPVTKKEKAVRETYTIVDENTRKMEMFDVDYSGKEYKSMEIMMTRKK"
     gene            2873..6526
                     /gene="purL"
                     /locus_tag="P5335_05725"
                     /gene_synonym="purI"
     CDS             2873..6526
                     /gene="purL"
                     /locus_tag="P5335_05725"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRZ74408.1"
                     /translation="MIHFFGNESATVFAVQSQNELSAQDISKLNWLFGNTNKIEKSVL
                     TDFFVGPRAAMVTPWSTNAVEITQNMGISGIIRIEEFHKAESNDTDFDPMLFQKYSEL
                     HQEIFTINIQPESIKEIDDIAAYNKKEGLSLSEEEVNYLNTLAIKLKRNLTDSEVFGF
                     SQVNSEHCRHKIFNGTFVIDGEEKPSSLFKLIKKTAAENHNDIVSAYKDNVAFIKGPT
                     VEQFAPKSADKPDFYQTKAFDSVISIKAETHNFPTTVEPFNGAATGSGGEIRDRLAGG
                     QGSLPLAGTAVYMTSYSRLEETRPWENAMNERKWLYQTPMDILIKASNGASDFGNKFG
                     QPLITGSVLTFEHEEEARKLGFDKVIMLAGGIGYGKAEQAQKHEPKIGDKIVILGGEN
                     YRIGMGGAAVSSADTGAFGSGIELNAIQRSNPEMQKRAANAIRGLVESDENPIVSIHD
                     HGAGGHLNCLSELVEATGGLIDLDKLPIGDPTLSAKEIIGNESQERMGLVIGQKDVDI
                     LHRIADRERSPMYEVGDVTGDHRFTFKSKSTGAKPMDFELADMFGSSPKVIMNDTTVE
                     RNYTEINYQESEFTTYLEQVLQLEAVACKDWLTNKVDRCVGGKVAKQQCAGPLQLPLN
                     NVGVMALDYKGKEGIATTIGHSPISALIDPKAGSRNAIGEALSNIVFAPLKDGLKSVS
                     LSANWMWACKNEGEDARLYEAVEACSDFAIELGINIPTGKDSLSMKQKYTDMEVIAPG
                     TVIISAAGNCNDITKVVEPVLQKDAGSIYYINLSQDEFKLGGSSFAQVRNAIGKETPT
                     IKNAAFFKKAFNVVQELIKNDEIAAGHDIGSGGLITTLLEMCFADNQLGAKLDFTAFN
                     QKDILKILFAENIGIVLQAKDDVLFESFLKEENIEFFKLGNVVENNWLEIGNWKLDIS
                     KYRDDWFKTSYLLDQKQTKNGKAAERFANYKMQPLAYSFPSHFTGQKPSIDDSKPRPK
                     AAIIREKGSNSEREMANAMYLAGFDVKDVHMTDLISGRENLEEIQFIGAVGGFSNSDV
                     LGSAKGWAGAFLYNEKAKTALDNFFKREDTLSVGICNGCQLFMELEVINPEHEIHGKM
                     HHNDSHKHESIFTSVKVQENNSVMLKTLAGTTLGVWVSHGEGKFKLPYEESQYNIVAK
                     YGYENYPANPNGSDFNTAMMCDKTGRHLVMMPHIERSTFPWNWAHYPKDSNDEVSPWL
                     EAFVNARLWIEKH"
     gene            6597..8534
                     /locus_tag="P5335_05730"
     CDS             6597..8534
                     /locus_tag="P5335_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRZ74409.1"
                     /translation="MKKFVYLKPILSFFCLGLIFTTLSRIFLFFFFNERIIETENYWY
                     IFPIGLRFDIILLSYISFLPLLIISFIPEKSIKYIQKPLNFYFIFFLFLILVTELTTT
                     NFISQYDTRPNRIFLDYLIYPKEVFGTLLKSYLPSLIIIGILLIVGIYFGVKKSKTLF
                     LIKAVSFKTRLLLFPAILFLLFLGARSSLTSKRPINASNAVFSTDQMTNSLGLNSLYT
                     VGFAAYSIKNEENVMKMYGTMDINESYQRVKKYMTTDNNNFTFDSIPLLHGNKSDSIS
                     TKKYNVVIFLQESLGAEFVGSLGGLPLTPEFDKLTKEGLLYTNLYCTGTRSVRGIEAV
                     VAGFPPTPSESVVKLSNSQKDFFTLATAFGNEGFDTSFIYGGMANFDNMASFFNGNGF
                     KNIIDETDFDSDKNKYALKGTWGYSDEDLATKANQYFKSLGDKPFFSMMFSTSNHEPF
                     EFPDSRIQLYDKKKQTVNNAIKYADFSIGKFIELAKKEAYFKNTIFVIIADHNTRTYG
                     KNLIPIKKFRIPALIIGPNVPKGETVDLQSSQIDIPPTILSFSGLKTETPMIGRSLFE
                     ITKNSKGRSMMQFHDNFGFRVQNEIVVLQPKSKPVQFKVINDSILIPSKLDTELAKDA
                     LAHLIVAENLYKTKTYRLKNK"
     gene            8589..10364
                     /locus_tag="P5335_05735"
     CDS             8589..10364
                     /locus_tag="P5335_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRZ74410.1"
                     /translation="MIQITRLFDFPYYQLEKNNIPDCFVTKYNGEWVKTSTQEYIDKS
                     NTISRALLRLGVNKNDKIAIITSANRTEWNIMDIGALQVGAQTVPIYPTIAPEDYQYI
                     LNHSESKYCFVSDKVVFDKLITIKDNVPSLQKIYSFDEIEGCNNWKELLELGADTTNQ
                     DVVEDRKNNVKPEELATIIYTSGTTGRPKGVMLSHNNIVSNVLDSAPRIPFKEGTSKA
                     LSFLPICHIFERVILYIYQYYSVSVYFAESIDKLTVNLKEVHPNVMTVVPRLLEKVYD
                     SIHAKGTDLKGIKKLLFFWAITLGLKYKPYGENGWFYEKQLAIARKLIFSKWKEGLGG
                     NLEVMVCGSAALQHRLSRIFAAANIPVMEGYGLTETSPVISVNDMRNGLFRVGTVGKL
                     IKNVEVKIAEDGEILCKGPNVMMGYYKDEALTSEAIKGGYFHTGDIGEFDVDGFLRIT
                     DRKKEMFKTSGGKYIAPQIIENRMKQSFFMEQVMVIGDGEKMPAAFIQPNFGFVREWA
                     HRHDFEITGTNEELIANPKVIERFQQEVDHANEKFGNWEKVKRFELTPDVWSIEAGHL
                     TPTLKLRRKIVKEKYQHLYDKIYNS"
     gene            10438..10821
                     /locus_tag="P5335_05740"
     CDS             10438..10821
                     /locus_tag="P5335_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74411.1"
                     /translation="MKMKNFLVAGIVGGIVDFLLGWVFYGMLFSDLYPEGENMNLGFI
                     ALGCLTYGLFIAYIFTKWAGIKHASTGAKAGAVIGFFYALSMNFYMYSSREVNYQNIV
                     IDVAITIVMSAAVGAVIAMVNDKLK"
     gene            complement(10949..12046)
                     /locus_tag="P5335_05745"
     CDS             complement(10949..12046)
                     /locus_tag="P5335_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRZ74412.1"
                     /translation="MKKTSVQTVLLNLFMGLVFVPLLMAQDAKTSANVVTSKFDFIPG
                     EKVVFYDDFTNENVGDFPVQWNTNSSGEIVTVNNFPGRWFQFIGGGYYIPEAKEKFTD
                     NFTVEFDLIPSTTIEGVNYLFNMQFLFVSGTLNNPNEGGAIPGKAGTSMTADYENINW
                     TNWSESNEGYKDNGNSSFMFMTGEKYHVAFWIQKQRIRMYANDKKVLDLPRGLIEGYV
                     YNIFRIQTSNEINPLISNFRIAAGLPDMRNKLITEGKLVSYGIQFDVNSDKIKPESYA
                     TIKEIAQVLKDNPTVKIKIIGHTDSDGDDNSNLDLSKRRAISVKTELTKSFSIDSSRM
                     ETDGKGESQALAPNDTGLNKAKNRRVEFVKL"
     gene            12219..12359
                     /locus_tag="P5335_05750"
     CDS             12219..12359
                     /locus_tag="P5335_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74413.1"
                     /translation="MEKIAILNLRLFDELEVDYSLFFNVKNNSIAPFGLGDIKIKSST
                     IA"
     gene            complement(12361..13185)
                     /locus_tag="P5335_05755"
     CDS             complement(12361..13185)
                     /locus_tag="P5335_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74414.1"
                     /translation="MKFKKGVLPFVLMLTLLGFSQNNETDKVLEEGKKLYRLEKAAWY
                     GVDDFLERFKSKQDSLAGYLSYFNDQGNVITIFYGRDTSQILVRYEFENLPTKKPLHI
                     DTRNRTTEPLEKILIEMRQNAKKRVKANISGFYKFYDETAMSYIPVFSKNERKVYVIT
                     ASKKKGEITLGNDYLLHYNNNNKFVSEEKFHHSLIRLQAKSGDRKNKLIETTHTNVIS
                     DFIDATDICSLLLYKDFVEWKKHIVLSDKQVTVFMLDTETLYTMTRKDWDRAAAAK"
     gene            complement(13204..13665)
                     /locus_tag="P5335_05760"
     CDS             complement(13204..13665)
                     /locus_tag="P5335_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRZ74415.1"
                     /translation="MSTLVLKRTTSNDPDFKALVVELDKDLKIRDGEEHSFYAQFNGI
                     DTINHVVVGYINQLPVGCGAVKAYSENAMEVKRMFVPLHERGKGIASQVLAELEVWCK
                     ELGYKECVLETGIKQPEAIALYKRNGFHIIPNYGQYVGVENSICFNKLLTP"
     gene            complement(13673..15265)
                     /locus_tag="P5335_05765"
     CDS             complement(13673..15265)
                     /locus_tag="P5335_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ74416.1"
                     /translation="MKKIVMLIGLFSVSCFAQAPGIQWQKSFGGSGEDFAKAIVQTSD
                     GGYVFGGDTNSSDGDVVGYPWSYSVTRRDYWIVKTDAEGNIAWQKVYGGFYNDLLTSI
                     NSTSDGGYIVSGYSESSDGTVLGHIGNYDYWLLKLNALGEIEWQKTLGGTNEDYATGI
                     IQTTDGGYITIGHTKSTNGGVSGNHGFFDVWIVKLSSAGAIEWRKAYGGSADDKISDA
                     YGNRAIIQTLDGGYIFTAKTKSSNGDVTTNQGLSDAWVVKINNLGTIEWQKTFGGASD
                     DFAHSVIQTNDGNYVITAGSASTTGDFPTNYGATDLWVIKINPTGAVLWQSKFGGTNS
                     ESYQYSSVIETNDNGLLVCSYTTSTNIDATGNHSFGSPDAWLVKLNSAGVKQWHRCFG
                     GRNWDEGHQILQTADEGYMIAGYNSNHNDPSGADWTFNHGTYDAWLIKLNPETLSQPD
                     FTAPDEQLLVYPNPSRKLLYLKAPEQVEINKIIITDVSGKECLVKTNQLSVLEIEQLS
                     AGIYFIQAFSNKGVFNTKFIKE"
BASE COUNT         5091 a         2717 c         2714 g         4960 t
ORIGIN      
        1 acactttccg ctaaagcaac agaagctcca ccatgtagca atcccattgg ttgatgaaca
       61 ttggggttta ccggcatttt agcttttaaa aaaccttccc cggcatctat aaattcaatt
      121 tcgagtgttt ccattaaggt gtttttgctc cattcgttac acttttgcaa cactttttct
      181 ttatcaaaag ccatttttta catttttggt aaaattacta aagaaatata gaatttagtc
      241 gctcatgata aactaaaatc attttttaat cgtttaaaga atagttttgt caattcaatt
      301 tattcgttaa ttttaccaaa aatcaggctc atgcgtaatt ctctttttct actatttatt
      361 gtctttgcca tctctattac ttcttgtcgg aacgactttg aatttgaacc aagtaccggt
      421 ggattagaat tttcaagaga cactatttat atggacacgg tttttactaa tgtaagttca
      481 agtacgtata ccttaaaagt atataaccga tcgaacaaag acatcagcat tcctcaaatt
      541 aaattgggaa aaggattaga ttctaaatac cgtatgacgg ttgacggcat ggtgggaaac
      601 aaccgtattt ttgaaaatgt agaaatgctt gctaaagaca gtatgtacat ttttattgaa
      661 actaccgttg atattgcaca agcaaatcct tccgattttt tatataccga tcaaattgaa
      721 tttggttccg gttcaacact gcaaaaagta gaattagtta ccttaattca agatgcttat
      781 tttctttatc cacaacgttt tgatgatgga acaaccgaaa cattacccat tggtgatgag
      841 gaagtttatg gattttattt agatgaaaat gacccaacaa atggtaatga atatgtttgg
      901 aacaattcaa aaccgtatgt gatttatggg tatgccgcag ttccggcaaa caaaacatta
      961 accgttcaag ccggtgccca aattcatttt catgccgatt ccggtttaat tattggcgag
     1021 aatggttcta ttaaagttaa tggtttacct tcaaataatc cagaaaatct acttgaaaat
     1081 catgtggttt tcgaaggtga tcgcttagaa cctttatatg atgatgttcc cggtcagtgg
     1141 ggaacgattt ggctcacaca aggcagtaaa aataatgaat ttaccaattg tcttattaaa
     1201 aatggaacag ttggaatact agtaacaggc aacacaaact tagctaatcc cgaagccgat
     1261 gttaagttga aaaacgtgca aatttatgac cattctaacg taggtgtttt agctagaacc
     1321 ggttacattt ccggagaaaa tgtcgtcatc aatagtgctg gtcaagcggc tttagcttgt
     1381 tcttatggag gaaaatacaa cttcaatcat tcaaccttta acaataattg gcccagttca
     1441 aaacaagttt ctgttttact gaataattat atagaagggg ctgttcccga aattcaaccg
     1501 atggaagagg caacttttac caattgtatc atttatggtt caaaccaaat tcaaatgatt
     1561 attgacaaaa aagaatcacc tatttttaac tataaatttg accactgttt gattaaattc
     1621 aataatgtaa acaatgtttt cacaaacaat ccattatatc aatttactac ggatacagaa
     1681 cattatacta attgtttgat tgcgacaaac tcagcagtat tcgatcctga cttttttgat
     1741 ataaattcca atcaattaat tattggtgcg gattcagcag ctattggtca agcaaacttc
     1801 agcttttctt ccggcacatt tgatatacta ggaaaaccaa gaatttcacc tgctgattta
     1861 ggtgcttata attttgttgt ttttgaagaa gattaannnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnaatt tcacctgctg atttaggtgc ttataatttt gttgtttttg
     2041 aagaagatta atttttattt cataatttta ctaaactaac cttaaaataa ttaaattatg
     2101 aaaattaaat ttttattcat tattgcaatt atttcgcttg tttcttgtgg aaaaaaagaa
     2161 gaaactaaac ctgaaactac cacagctaaa gacacaacca ctgtggagga aatagcagta
     2221 accactccag attcaacaac gatagcaaaa gcatgggaag attatatgac tcccgcagaa
     2281 caacataaaa tgcttgctct tgaaaatggt atttggaatg aagaaatgac tatgtggatg
     2341 gaacctggag ctgaaccgat gaaaaacacc atgacagccg tttctaaaat gatttacggt
     2401 gatcgttatc aagaaactgt tcacaaaggc gattttatgg gaatgccttt tgaaggaaaa
     2461 agcacgttgg cattcaacaa agcttcacaa gaatatactt caacttggat tgacaacatg
     2521 agtaccggaa ttatggttat cagcggaaaa tatgatgaag caactaaaac tatcaatttt
     2581 tcaggaacta cggtggatcc ggttactaaa aaggaaaaag cagttcgtga aacatatacc
     2641 attgtggatg aaaacacccg taaaatggaa atgtttgatg tagattacag cggtaaagaa
     2701 tacaaaagca tggaaatcat gatgaccaga aaaaaataat ttaaccaaat ttttaatacc
     2761 aaaagactgt tttcacagtc ttttttttgt ttacaactct tgcaaaaata tactttgaaa
     2821 ctatcaattt tagatgtaaa tttgcaacta caacaacaca aataatttta ctatgattca
     2881 tttcttcgga aacgaaagtg ctactgtttt tgccgttcaa tcgcaaaacg aactttcggc
     2941 tcaggacatc tcaaaattaa actggctttt tggcaataca aataaaatag aaaaatccgt
     3001 ccttacggat ttttttgttg gaccacgtgc cgcaatggtc accccttgga gtaccaatgc
     3061 tgtagaaatc actcaaaaca tggggatttc cggaattatt cgtatagaag aatttcataa
     3121 agcggaaagt aacgatactg attttgatcc gatgttattt caaaaatata gcgaattaca
     3181 tcaggagatt tttaccatca atatccaacc ggaatccatc aaagaaattg atgacatagc
     3241 cgcttacaac aaaaaagaag gtttatcttt aagtgaagaa gaagtcaatt atttgaatac
     3301 gctagccata aaactaaaac ggaatttaac cgattctgaa gtttttggtt tctctcaagt
     3361 aaattcagaa cattgccgtc ataaaatttt caacggaaca tttgtgatag atggtgaaga
     3421 aaaaccttct tccctattta aattaatcaa aaaaacagct gccgaaaatc ataacgatat
     3481 tgtttcagca tacaaagata atgttgcatt tatcaaagga ccaactgtag aacaatttgc
     3541 tccaaaaagt gccgataaac ctgattttta tcaaaccaaa gctttcgatt ctgttatttc
     3601 tatcaaagct gaaacgcata attttccaac aacggttgaa cctttcaatg gtgccgctac
     3661 cggttcaggt ggagaaatca gagacagact tgccggtgga caaggttcac ttccattagc
     3721 aggaactgcg gtttatatga cttcttactc tcgattggaa gaaacccgcc cgtgggaaaa
     3781 tgccatgaat gaacgcaaat ggttgtatca aacacctatg gatattttaa tcaaagcatc
     3841 caatggagct tctgattttg gaaataaatt tggtcagccg ttaattacag gttcagtttt
     3901 gacttttgaa cacgaagaag aagcaagaaa acttggtttc gataaagtaa tcatgttagc
     3961 aggtggtatt ggctacggaa aagctgaaca agcccaaaaa cacgaaccaa aaataggcga
     4021 taaaatcgtg attcttggtg gtgaaaatta ccgaattgga atgggtggag cagccgtttc
     4081 ctccgctgat accggagcat ttggttcagg aatagaatta aacgccattc aacgttctaa
     4141 tccggaaatg caaaaaagag cagccaatgc tattcgaggc ttggtagaaa gtgatgaaaa
     4201 cccaattgtt tccattcacg atcacggtgc aggcggacac ttaaattgtt tatcagaatt
     4261 agtagaagcg accggtggat taatcgattt agacaaattg ccaattggcg accctactct
     4321 ttctgccaaa gaaattattg gtaacgaaag tcaggaacgc atgggattag taattggaca
     4381 aaaagatgtt gacatactac atcgaattgc tgatagagaa cgttccccga tgtatgaagt
     4441 aggagatgtt actggagacc atcgtttcac gtttaaatca aaatcaaccg gagctaaacc
     4501 gatggatttt gaattggctg acatgtttgg aagttctcct aaagttatta tgaatgatac
     4561 tacggtagaa agaaattata ccgaaattaa ttatcaagaa tctgaattta caacctattt
     4621 agaacaagtt cttcaactcg aagctgttgc ttgtaaagat tggttaacca acaaagtcga
     4681 tcgttgcgta ggaggaaaag tagccaaaca acaatgtgca ggaccactac aattaccctt
     4741 aaacaatgtc ggtgtaatgg cgttggatta caaaggaaaa gaaggaattg ccactaccat
     4801 tggacactcc cctatctcgg ccttaattga tccaaaagcc ggaagtagaa acgccattgg
     4861 agaagcttta tccaacattg tttttgctcc acttaaagat ggtttaaaaa gtgtttcgct
     4921 ttctgccaac tggatgtggg cttgtaaaaa tgaaggtgaa gatgctcgtt tatatgaagc
     4981 cgtagaagct tgttctgatt ttgctatcga attaggaatc aacattccaa ccggaaaaga
     5041 ttcgctttcc atgaagcaaa aatataccga catggaagtg attgctcctg gaacggtcat
     5101 tatttctgct gccggaaatt gcaacgatat tactaaagta gtggaaccgg ttttacaaaa
     5161 agatgcaggt tcaatttatt atattaattt atcacaagac gaattcaaat taggtggttc
     5221 ttcgtttgca caagtgagaa atgctattgg taaagaaaca ccaaccatca aaaatgctgc
     5281 attcttcaaa aaagcattta atgtagttca agaattaatc aaaaatgatg aaattgctgc
     5341 cggtcatgat attggtagtg gcggattgat taccacttta ttagaaatgt gttttgcgga
     5401 taatcagctg ggtgctaaac ttgattttac tgctttcaat caaaaagaca tattaaaaat
     5461 tctatttgca gaaaatattg gaatagtact tcaagcaaaa gatgatgtcc tttttgaatc
     5521 ctttttaaag gaagaaaata ttgagttttt caagttaggc aacgtcgttg agaataattg
     5581 gttagaaata ggaaactgga aattagacat ttctaaatac agagacgatt ggttcaaaac
     5641 atcctattta ttagaccaaa aacaaaccaa aaacggaaaa gcagcagaaa gatttgcaaa
     5701 ttataaaatg caacctttag cctattcatt tccttctcat tttacaggtc aaaaaccaag
     5761 tattgacgat tcaaaacctc gtccaaaagc cgctattatt cgtgaaaaag gaagtaattc
     5821 tgaacgcgaa atggcaaacg caatgtattt agccggattt gatgtaaaag atgtgcacat
     5881 gaccgactta atttccggac gggaaaactt agaagaaatc caatttattg gagcagtagg
     5941 tgggttttca aattcagatg ttttgggttc tgccaaaggt tgggcgggag cctttttata
     6001 caacgaaaaa gcaaaaacag cgttagataa tttcttcaaa cgagaagaca ctctttcggt
     6061 cggaatttgt aatggttgcc aattgtttat ggaattagaa gtcattaatc cggaacatga
     6121 aattcacgga aaaatgcatc ataacgatag ccacaaacat gaaagtattt ttacttctgt
     6181 aaaagttcag gaaaataatt ctgtgatgct taaaacatta gccggaacaa cattaggcgt
     6241 ttgggtttcg catggtgaag gaaaatttaa attaccatac gaagaaagtc aatataatat
     6301 tgtggccaaa tacggttatg aaaattatcc tgccaatcca aacggttccg atttcaacac
     6361 agcgatgatg tgcgacaaaa ccggtagaca tttagtgatg atgccacaca ttgaacgttc
     6421 cactttccct tggaattggg ctcattatcc aaaagacagt aacgatgaag tttcaccttg
     6481 gctagaagcg tttgtgaatg ctcgtttgtg gattgaaaaa cattaagtaa atataaattt
     6541 tttctaaatt tttgtacaat attttaaaag ctatatttgc ttcataaaat agaaaaatga
     6601 aaaaatttgt atatctcaaa cctatcctat cctttttctg cttggggtta atttttacaa
     6661 ctctaagcag aattttttta ttcttttttt ttaatgaaag aataatcgaa accgaaaatt
     6721 attggtatat attcccaatt ggattacggt ttgatatcat tttgttgagt tatatttcat
     6781 ttctacccct attgataata agttttattc cagaaaaaag tataaaatat atacaaaaac
     6841 cattaaattt ttatttcata ttttttttat ttttaattct ggttactgaa ttaactacaa
     6901 caaatttcat aagtcaatat gatacacggc caaatcgaat ttttttagat tatttaattt
     6961 atccaaaaga agtttttggt actttattga aaagttattt accttctctt ataattattg
     7021 gaattttact tattgttgga atttatttcg gtgttaaaaa aagtaagaca ttattcctca
     7081 tcaaagcggt ttcattcaaa actaggttat tattatttcc tgccatttta tttctgctat
     7141 ttttaggagc aagatcgagt ttaacgtcta aaagacctat caatgccagt aatgctgttt
     7201 tttcaacaga ccaaatgaca aattcattag gattaaattc tttatatact gttggatttg
     7261 cagcttattc cataaaaaat gaagaaaacg tcatgaaaat gtatggaaca atggatatca
     7321 atgaatcata ccaacgtgta aaaaaataca tgacaactga taacaacaat ttcacatttg
     7381 atagtattcc gttattacat ggtaacaaat cagatagtat ttcaacaaaa aaatataatg
     7441 tggtcatctt tttacaagaa agtttaggag ccgaatttgt aggttctcta ggaggacttc
     7501 cgttaacgcc tgaatttgat aaactaacaa aagaaggttt actttataca aatttatatt
     7561 gtactggaac aagaagtgtt cgaggaattg aagctgtagt agctggattt cctcccactc
     7621 cttctgaaag cgtggtaaaa ttaagcaatt ctcaaaaaga cttttttaca ctggctactg
     7681 cttttggaaa tgaaggattt gataccagtt ttatttatgg tggaatggca aactttgata
     7741 acatggcttc ttttttcaat ggaaatggtt tcaaaaatat catcgatgaa actgattttg
     7801 attcagataa aaataagtat gctctcaaag gaacttgggg ttattctgac gaagatttag
     7861 ctacaaaagc aaatcaatat tttaaatcat tgggagataa acctttcttt tcaatgatgt
     7921 tttcaacttc aaaccatgaa ccttttgaat ttcctgatag tagaattcaa ttgtatgaca
     7981 aaaagaaaca aacagtcaat aacgctatta aatatgctga tttcagtata ggtaaattta
     8041 tagaattagc aaaaaaggaa gcctatttta aaaataccat ttttgtaatt attgccgacc
     8101 ataataccag aacctatggc aaaaatttga taccaataaa aaaattcaga attcctgcct
     8161 taatcattgg tccaaatgta ccaaaaggag aaacagttga tttgcaatcc agtcaaattg
     8221 atattccacc aacaatttta agttttagcg gattaaaaac tgaaacaccg atgataggac
     8281 gaagtctatt tgaaataaca aaaaattcta aaggtagaag catgatgcag tttcatgata
     8341 attttggttt tagagtgcaa aacgaaattg ttgttttaca accaaaatcc aagccggttc
     8401 aatttaaagt gataaatgac agcatattaa taccctctaa attggataca gaattggcca
     8461 aagatgcttt agcccaccta attgttgctg aaaatctata taaaacaaaa acttacagac
     8521 taaaaaataa ataattaaat atgattatta aattaagtta cttattttag tatcaaaatt
     8581 atttaataat gattcaaata actcgtcttt tcgactttcc atactatcaa cttgaaaaaa
     8641 ataatattcc cgattgcttt gttactaaat ataatggtga atgggtaaaa acctcaactc
     8701 aagaatacat agataaatca aatactatct ctagggcttt attgcgttta ggtgtcaata
     8761 aaaatgataa aattgccatc atcacttctg ccaacagaac tgaatggaac atcatggata
     8821 ttggtgctct tcaagtaggt gcacaaactg ttcctatcta tccaactatc gctccggaag
     8881 attaccaata tattctaaat cattctgaat caaagtattg ttttgtctca gataaagttg
     8941 tttttgataa actaataacc ataaaagata atgttccctc gttacaaaaa atctattctt
     9001 tcgatgaaat cgaaggatgt aacaattgga aagaactgtt ggaattaggt gccgatacaa
     9061 ctaaccaaga tgtagttgaa gacagaaaaa acaacgtaaa accagaagaa ttagccacca
     9121 ttatttatac ctcaggaacg acaggaagac ctaaaggagt aatgctgtcc cacaacaaca
     9181 ttgtttcaaa tgtgttggac agtgctcccc gaattccgtt taaagaaggt accagtaaag
     9241 cattgagttt tttacccatt tgtcatattt tcgagcgggt tatcttatac atctatcaat
     9301 attattctgt ttcggtttat tttgcagaaa gcattgataa attaacggtt aacctaaaag
     9361 aagtgcatcc caatgtaatg actgttgttc caagactttt agaaaaagtt tacgacagca
     9421 tccatgctaa aggaaccgat ttaaaaggaa ttaaaaagct actgtttttc tgggcaataa
     9481 cgctcggatt aaaatataaa ccctatggcg aaaatggttg gttctacgaa aaacaacttg
     9541 ctattgcccg aaaactaatt ttcagtaaat ggaaagaagg attaggcggt aatttagagg
     9601 tgatggtttg cggtagtgct gccttgcaac accgtttatc ccgcattttt gctgctgcca
     9661 atattccggt tatggaagga tacggactaa ccgaaacatc gccggttatc agtgtaaatg
     9721 atatgcgaaa cggacttttc cgggtgggaa ccgttggaaa attaatcaaa aatgtggaag
     9781 taaaaattgc tgaagatggt gaaattctat gcaaaggtcc aaatgtgatg atgggttact
     9841 acaaagatga agcattaacc agcgaagcta taaaaggggg ttattttcat accggagata
     9901 ttggcgaatt tgacgttgat ggtttccttc gaatcaccga ccggaaaaaa gaaatgttta
     9961 aaacttccgg tggaaaatat attgctccgc aaatcattga aaacagaatg aaacaatcct
    10021 tttttatgga gcaggtaatg gtcattggtg atggcgaaaa aatgccggct gcctttattc
    10081 aacctaattt tggcttcgtt cgtgaatggg ctcatcgtca tgattttgaa attacaggaa
    10141 caaatgaaga acttattgca aatccaaaag taattgaacg gttccaacaa gaagttgacc
    10201 atgccaatga aaaatttggg aattgggaaa aagtaaaacg ctttgaactc actcccgatg
    10261 tttggtccat tgaagccgga catcttacgc caaccttaaa acttcgtcgt aagattgtga
    10321 aagaaaagta ccaacatttg tacgataaaa tatataattc ttaatttttt atccttttaa
    10381 ttaacagttg ttcaatcttt tttataaatt tatagttcta accaaaaaca tttatttatg
    10441 aaaatgaaaa actttttagt tgccggaatc gttggcggaa ttgtcgactt ccttttagga
    10501 tgggtattct atggcatgtt atttagtgac ctttacccgg aaggagaaaa catgaacttg
    10561 ggtttcattg ctttgggttg ccttacgtat ggactcttca tagcttatat ttttacgaaa
    10621 tgggcaggaa taaaacatgc ttcaacaggt gcaaaagcag gagcagttat tggatttttc
    10681 tatgcgttat ccatgaattt ctatatgtat tcttctaggg aagtaaacta tcaaaatatt
    10741 gtaattgatg ttgccattac tatcgtcatg agtgctgctg ttggagctgt tattgctatg
    10801 gtgaatgaca aattaaaata ataaacatac cattatttac caaaaaaact cccgtaatgg
    10861 gagttttttc atttaaagtt attctattga ctatgtgatt ccaattaaga aagaatccaa
    10921 atttatttta ccatctaatt attaaaaatt atagtttaac aaattcaacc cgtctgtttt
    10981 ttgctttgtt gagtccggta tcgtttggag ctaaggcttg actttcacct tttccatctg
    11041 tttccattcg ggaagaatct atactaaaag atttagtaag ttctgtttta actgaaattg
    11101 ctctgcgttt agacaagtct aggttgctgt tgtcatcacc atcactgtct gtatgaccaa
    11161 tgattttaat tttaacggtt ggattatctt ttaacacctg agcaatctct ttgattgtag
    11221 cataagattc tggtttaatt ttatctgaat taacatcaaa ctgaatacca taactcacta
    11281 acttaccttc tgtgattaat ttgtttctca tatcaggcaa acctgctgcg atacggaagt
    11341 ttgaaatcaa tggattaatt tcatttgatg tttgtattct gaatatatta taaacatatc
    11401 cttctattaa acctctgggc aaatccaata cttttttatc attggcatac attcgaatac
    11461 gttgtttttg aatccaaaaa gcaacatgat acttttctcc ggtcatgaac ataaaactgg
    11521 aattaccatt gtctttatat ccttcattac tttctgacca attggtccaa ttgatgtttt
    11581 cataatcagc tgtcatgctg gttcctgctt tcccgggaat agctcctcct tcgttcgggt
    11641 tatttaacgt tccggaaaca aaaagaaatt gcatattgaa taaataattt acaccttcaa
    11701 tagttgtaga gggaattaaa tcaaactcta ccgtaaaatt atctgtaaac ttctctttag
    11761 cttccggaat ataatatcct ccccctataa actgaaacca tctacctgga aaattattaa
    11821 ctgtcacaat ttcaccgctg ctattggtat tccattgcac cggaaaatcg ccaacattct
    11881 cattggtgaa atcatcataa aaaacaacct tttcacccgg aataaagtca aacttagaag
    11941 tgactacatt tgcacttgtt tttgcatcct gagccatgag caagggcaca aaaactaacc
    12001 ccataaaaag gttaagtaat acggtttgaa cactcgtttt tttcatgttt ttatagttat
    12061 aaatgaatat tcactgtact ctttaaatca gatagtgatt actcaactaa gtgacttggc
    12121 tgtttgaaga ataccaagta tgttgaaaca attggttttc atcaaactga aaaacctatt
    12181 ttgaaatcaa ctttttacat cccaattttt tcatgaagat ggaaaaaatt gccatcttaa
    12241 atttacgact atttgatgaa ttagaagtgg attattcctt gttttttaat gtgaaaaaca
    12301 attcaattgc tccctttggt ttgggagaca taaaaataaa aagtagtact atcgcctgag
    12361 ttatttagct gctgctgccc tatcccaatc ctttcgggtc atggtgtaaa gggtttcggt
    12421 gtccagcata aaaacagtta cctgtttatc actcagtaca atgtgttttt tccattctac
    12481 aaaatctttg tataacaaga gtgaacaaat atctgtggca tcaatgaagt cactgattac
    12541 gttggtgtgt gtcgtttcaa tcagcttgtt ctttctatca cctgattttg cttgtaagcg
    12601 aattaaggaa tgatgaaact tctcttcaga gacaaactta ttgttgttgt tgtaatgtaa
    12661 aaggtaatca ttacctaagg tgatctcccc cttctttttt gaggccgtta tcacatacac
    12721 ttttcgttca tttttactga acacgggtat gtaactcata gccgtttcat cataaaactt
    12781 ataaaaaccg ctaatgtttg cttttaccct tttctttgca ttctgacgca tttctattaa
    12841 tattttctcc aagggttctg tggttctgtt gcgggtgtca atgtgtaagg gctttttagt
    12901 cggtaaattt tcaaactcat agcgaaccaa tatctgagac gtatcacgtc cgtaaaagat
    12961 ggtaatcacg ttgccttggt cattgaaata agacaagtat ccggctaaac tatcttgctt
    13021 tgatttgaat cgttccagaa agtcatctac gccataccac gcggctttct ccaaacggta
    13081 tagtttcttt ccttcttcta acaccttatc cgtttcattg ttttgggaaa accctagtaa
    13141 ggttaacatt aaaacaaacg gtaatactcc ttttttaaac ttcatagcct tttaaataaa
    13201 acgttaaggg gtcaataatt tattaaaaca aatgctattt tctacaccaa catactgccc
    13261 ataatttggt atgatatgaa aaccattccg tttataaagt gcgatggcct ccggttgctt
    13321 aattccggtt tccaaaacac attctttgta acccaactct ttacaccaca cttctaattc
    13381 agctaatacc tgagaagcta ttcctttccc tcgctcatgt aaaggaacaa acatacgttt
    13441 tacttccatt gcattttcac tataggcttt aacggcaccg caacccacgg gcaattggtt
    13501 tatataacca actactacat gatttattgt atctattccg ttaaactgag cataaaacga
    13561 atgctcttca ccatctctga ttttcaaatc tttgtctaac tcaaccacca aggctttaaa
    13621 gtccgggtcg tttgaagtag ttcgttttaa tacaagtgtg gacataacta atttactctt
    13681 taataaactt ggtgttgaac acgcctttgt ttgaaaaggc ttggatgaaa tagattcctg
    13741 ccgacaattg ctctatttct agtactgaaa gttggtttgt tttgaccaag cattctttac
    13801 cacttacgtc tgtaatgatt attttattta tttctacctg ttcgggtgct tttagataaa
    13861 gtagttttct ggatgggttt ggataaacca gcagttgttc atcgggagct gtgaagtccg
    13921 gttgtgataa agtttcgggg tttagtttga ttaaccaagc atcataggtg ccatgattaa
    13981 acgtccagtc tgctccactt gggtcattgt gattggaatt gtatcctgca atcatatagc
    14041 cttcgtcggc ggtttgtaaa atctgatgtc cttcgtccca gtttcttccg ccaaaacaac
    14101 ggtgccattg tttgactcct gcactgttta attttactaa ccaagcatct ggtgaaccga
    14161 aactatgatt tcctgttgca tcgatatttg ttgacgtcgt gtagctgcac accaataatc
    14221 cgttgtcgtt tgtttctata acggagctgt attgataaga ctctgagtta gtgcctccaa
    14281 attttgattg ccaaagtacg gctcctgttg ggtttatttt gattacccat aaatccgtag
    14341 ctccataatt cgtaggaaag tcgcccgtgg tggaagcact tccggctgta atgacatagt
    14401 taccatcatt agtttgaata acactgtgtg caaagtcgtc actggctcca ccgaaggttt
    14461 tttgccattc gattgttccg aggttgttga ttttaactac ccatgcatcg cttaatcctt
    14521 ggtttgtcgt aacgtcgcca ttggatgatt tagtctttgc tgtaaaaata tatccgccgt
    14581 cgagggtttg aatgatggca cgattaccgt aggcatcact tattttatca tcagcagagc
    14641 cgccataggc ctttctccac tctattgctc ccgcactgct taactttact atccacacgt
    14701 caaagaagcc gtggtttcct gacacaccgc cgttggttga cttggtgtgt ccaatagtaa
    14761 tatagccgcc atccgtcgtt tggataattc cggtggcata atcttcattg gttccgccga
    14821 gggttttttg ccattcgatt tctcccaagg cgtttaactt gagtaaccaa tagtcataat
    14881 tgccgatatg gcctaacacg gtaccatctg atgattcaga ataacctgaa acgatgtagc
    14941 cgccatcgga agtggagttt atgctcgtta agaggtcgtt gtagaaacca ccatatactt
    15001 tttgccaggc aatgttgccc tctgcatctg tttttacaat ccaataatct cttcttgtca
    15061 cactgtagct ccagggataa ccgactacat cgccatcaga agaattagta tcgccaccaa
    15121 atacatatcc accgtcggag gtttgaacga ttgctttagc aaaatcttcg ccggaaccgc
    15181 caaaggattt ctgccattga atgcccggtg cttgcgcaaa acaagacacg gaaaacaaac
    15241 caattaacat cactatcttt ttcatactgc aaaggaaggt aacttatttc aattattttc
    15301 acttttggtg gtggctgtat tccccataaa tagtagaaat gagcaccctt tgtgagagca
    15361 aatataaaat aagatttaag gtaaacacca ttctttttcg tagaaataac aatttaatga
    15421 tttaaaatca acccgattgg agtcaatttt gagctaaaaa agtagtgcag tttgtaaata
    15481 atgaacaact tgttttataa cactcctata acaccagtat aacttaggta taacttaggt
    15541 ataacttagg tataacttaa gaatacattc ttagtacact ag
//
LOCUS       DAOTCW010000050        15549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2191_length_15549_cov_46.534723, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000050 DAOTCW010000000
VERSION     DAOTCW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 15549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15549
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2191_length_15549_cov_46.534723"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..208
                     /locus_tag="P5335_05770"
     CDS             5..208
                     /locus_tag="P5335_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane or secreted protein"
                     /protein_id="HRZ74417.1"
                     /translation="MKLVLITIGLLALAFAGIAIKIWAKKDGKFSGTCASQSPFLNKE
                     GENCSFCGKTSEQMKDCSEPSHS"
     gene            223..1329
                     /locus_tag="P5335_05775"
     CDS             223..1329
                     /locus_tag="P5335_05775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRZ74418.1"
                     /translation="MLDILFYSLIGVVIIQLFYYILVFGNFSFTKSQKNNPKRIPISV
                     IVCAKNEADNIKKFVPLLLEQNYPDFELVLIDDSSTDETLELFEQFEKQDSRVKLVKV
                     KNNEAFWGNKKFALTLGIKAAKNEYLLFTDADCYPTSKEWIKEMSSNFTLKKTIVLGY
                     GAYEKIPDSFLNKLIRFETMLTATQFFGWAKMGKPYMGVGRNLAYKREEFFRVNGFID
                     HMKLRSGDDDLFINQAAEKENTTVCFSKESFTYSMPKTTYKEWFTQKRRHVSTATYYK
                     MFDRFQLGLFFLTQLLFLILAILLLSINFQWMFVLPVVLIRYTFAWISMGFAASKLNE
                     KDVIYWFPVMEIALIFTQLNVFFTNIFSKPVHWK"
     gene            1320..1868
                     /locus_tag="P5335_05780"
     CDS             1320..1868
                     /locus_tag="P5335_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRZ74419.1"
                     /translation="MEIKKIIEKAINGDQVAFTSLLDMYWNEVYGFMLKRTENETDAE
                     DITIETFSKAFDKIASYNSEFQFNTWLIAIAKNVHIDMLRKKKSTLFVDMNDEEDHQA
                     YNVADTTPSAEDKLITEQNLSRLLAYIKELKPHYQEVIQLRYFQELSYQEIAEHLDEP
                     LNNVKIKLLRAKKLLAEIILKR"
     gene            1947..2819
                     /gene="lipA"
                     /locus_tag="P5335_05785"
     CDS             1947..2819
                     /gene="lipA"
                     /locus_tag="P5335_05785"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRZ74420.1"
                     /translation="MNTVLENVQPPIGKPKWLRVKLPTGKKYTELRGLVDKYSLNTIC
                     TSGSCPNMGECWGEGTATFMILGNICTRSCGFCGVKTGRPETVDWDEPEKVARSIKIM
                     NIKHAVITSVDRDDLKDMGSIIWFETIQAIRRMNPETTLETLIPDFQGNERNIDRIIE
                     ANPEVVSHNIETVRRLTREVRIQAKFDRSLEVLRYLKAQGIKRTKSGIMLGLGETEEE
                     VMETMRELRAVNVDIVTIGQYLQPSKKHLQVKEFITPEQFKKYEDYGKELGFRHVESG
                     ALVRSSYHAQKHIL"
     gene            2838..3830
                     /gene="gap"
                     /locus_tag="P5335_05790"
     CDS             2838..3830
                     /gene="gap"
                     /locus_tag="P5335_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRZ74421.1"
                     /translation="MKKRIAINGFGRIGRNLFRLLLNHPTIEVVAINDLADTKTMAHL
                     LKYDSIHGILNATVSFDQENIIIDEKKIRFYREKDIQNLHWEAIDFVIEATGKFNTFD
                     EAYLHINAGAKKVILSAPPEDDLIKTVVLGVNESILDGSELIVSNASCTTNNAAPMIK
                     IIDELCGIEQAYITTVHSYTTDQSLHDQPHKDLRRARGASQSIVPTTTGAAKALTKIF
                     PNLVNKLGGSGIRVPVPDGSLTDITCYVSKAITIEEINTAFLMASQNNLKGILAYTED
                     PIVSVDILGNQNSCLFDAQLTSVIGKMVKVVGWYDNEIGYSSRIIDLIEYISNK"
     gene            3867..6047
                     /locus_tag="P5335_05795"
     CDS             3867..6047
                     /locus_tag="P5335_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRZ74422.1"
                     /translation="MKYFILTILFFTTAINAQKKTNAKVALDSTNYYIELANFHKKNN
                     NFRSSQDYIQKAIEFAEIKKDIQAQADAYSFLGSVYFELKKIEDAIQTYDKSIKLYST
                     QKPSSNQAYALYNLGVCYVEMKDYSKAEFYFKGAENIYEIIEIPEAKEMVNLQKGIVY
                     RAKGENQLALSILNTIIIKPDEEDHFKTKAEALYQIGLIEAENNRNNLAINYLTKAME
                     LSTKNKNFELKSKVAKSLSEVYEKMIDINNSFKYIKQHIRIRDSIKEIINKRVGTEDF
                     LSFKQAEQLKSIDQMNRENKEQQRANKFSKLISILSIALISILSLLSLSLYKNNIIRS
                     QSNKQLLDKNQELQIAKDKAEKASKARAEFLSTVSHELRTPLNAINGITHLLIEENPK
                     KSQLQYLTSLKFSGDYLLTFINDILEINRVESENIEIESINVNVKQLLSDLHNSFKDL
                     ASKNNNEFILEIDEKIPDHLIGDPTKLSQIFINLINNALKFTKNGKVSIGASVVKTEN
                     DISTVHFEIADTGIGIPIEKQSSIFESFSQGSVEINRKYGGTGLGLAIVKRLVSLLGG
                     EIQLVSSQGIGSTFSFDIDFKLGQQTFVEVKKQYDESIFVHKKVLVVEDNKINQMITK
                     KMLENKEIICELVDNGEEAIEMVRSNEYDLILMDVHLPGINGTEATEKIRVFNKTIPV
                     IALTAISLNENREMLLSFGMNDVVTKPFNPDNFYKVIANQLSSI"
     gene            complement(6034..7317)
                     /locus_tag="P5335_05800"
     CDS             complement(6034..7317)
                     /locus_tag="P5335_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRZ74423.1"
                     /translation="MKNNFAFLFTFLFSCILFAQDTYLHCGKIVDAQSGKILTDMTIV
                     VSGNKIIKVVNGYVAPINPNDIEVDLKNKFVLPGLIDMHVHLENEFNSTNYDAKYTDN
                     EADVAFASTRYARITLMAGFTTVRDLGGSGVNISLRKAVDKGIVVGPRIFAAGKSIAT
                     TGGHADPTNGSSRKLMGDPGPVEGVINSPEDAVKAVRQRYKEGADVIKITATGGVLSV
                     AKNGKNPQFTLDEIKAITATAKDYNMLTAAHAHGDEGMQRAILGGIKTIEHGTFMSEE
                     TMEMMKKYDAYLVPTITAGKEVAEKAKITGFYPEIVRPKALEVGPQIQNTFAKAYNRG
                     VKIAFGTDAGVFPHGLNAKEFGYMVEAGMPAKEAILAATITNAKLLGMENSLGQLKES
                     FLADIIAVDENPLLNIKTLENVIFVMKDGFIFKSN"
     assembly_gap    7404..7503
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7504..7691)
                     /locus_tag="P5335_05805"
     CDS             complement(<7504..7691)
                     /locus_tag="P5335_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRZ74424.1"
                     /translation="MWDKVQETVNYLKNKTNYTPEFGVILGSGLGSFTQDIEIEFTIP
                     YAEIPNFPVSTVQGHKGA"
     gene            complement(7663..8688)
                     /gene="lpxK"
                     /locus_tag="P5335_05810"
     CDS             complement(7663..8688)
                     /gene="lpxK"
                     /locus_tag="P5335_05810"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRZ74425.1"
                     /translation="MILLRKILFPFAILYGFITSIRNWCYDIGILKSYSFDIPIIAVG
                     NLSVGGTGKTPQIEYLIRLLSSNYQIAVLSRGYKRKSKGFLLADATSTSESLGDEPFQ
                     MHQKFPAIQVAVDANRKNGIEKLLSLKKKPELILLDDAFQHRKVKAGFYILLTAYSDL
                     YSEDFILPTGNLRESRNGAKRANIIVVTKCPPNLSEFEQNKCLNKLKVSENQSVYFST
                     ITYDESLSSEIDSRSVDSFRTVDKLLVAGIAKPEPFFAYLQTPNDEVLYYPDHHDFSE
                     TDIQTIINKAKKKPIITTEKDYVRLKGKLPKNQLYFLPIQSSFLSNSDVFNKKIIDYV
                     GQSSRNR"
     gene            8758..9852
                     /locus_tag="P5335_05815"
     CDS             8758..9852
                     /locus_tag="P5335_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRZ74426.1"
                     /translation="MKLNTIIEQLEMLAPLSYAEDFDNVGLLTGNSEQEINGILVCHD
                     VLENVLNEAIEKKCNLIVCFHPIIFSGLKKITGKTYVERVVIKAIKNDIAIYALHTAL
                     DNHQNGVNKIICNQLGLKNTKILIPKEKFIRKLVTYTILENHEELRNALFDAGAGNIG
                     NYEDCSFNSKGIGTYLGNENSNPEIGERFEFVENEEIKLEVTFEKQLESKILKALFKT
                     HCYEEVAYEIYELQNQHQNIGLGMIGELETELSELDFLQFVKDKMQCGSIRHSEFLHK
                     NIKKVAVLGGSGSFAISQAKKMGADAFLTADLKYHQFYEAENQLLLADIGHYESERYT
                     KNYIVDFLIKKIPNFAIILSEQNTNPVKYF"
     gene            9856..10635
                     /locus_tag="P5335_05820"
     CDS             9856..10635
                     /locus_tag="P5335_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74427.1"
                     /translation="MTNVKEMSVEEKLRALYDLQLIDSRIDEIRNVRGELPLEVQDLE
                     DEVAGLKARNEKLKADLEQIDDQIKGKKNAIEAHKEGIKKYTEQQKNVRNNREFNSLT
                     KEIEFQELEIQLSEKHIKEMKASVEYKKQQVADSQERLNAKQSHLEHKKAELGDIMAE
                     TQKEEEYLTKLSTEYASNIEPRLLAAYNRIRNSVRNGLAVVSIERGASAGSFFTIPPQ
                     TQVEIAARKKILTDEHSGRILVDNVLATEEKEKMESIFAKI"
     gene            10666..11514
                     /locus_tag="P5335_05825"
     CDS             10666..11514
                     /locus_tag="P5335_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRZ74428.1"
                     /translation="MNKTLKNIVTKSIGAYINLLSHTFPKSATKLAYKFFSEPRVGKL
                     NANSLPTILQKAEREIVQLENYQFPVYIWKGNETKILLVHGWESNAARWEPIFPFLQK
                     SGSTIIALDGPAHGLASGVEFSVPKYADFIHEVVLKHKPQIIIGHSIGGSACLYFQHH
                     FTTLSLEKMVLMGAPSDLEVLLENYAKLLGLNTKVIQLLDAYFLKRFNIKTKEFLGKK
                     LASTLSLNGIISHDREDDVVLFSESKKIAEGWEKATFIETKGLGHSMHDEKLYESIYS
                     FLFQKL"
     gene            11529..12305
                     /gene="rluF"
                     /locus_tag="P5335_05830"
     CDS             11529..12305
                     /gene="rluF"
                     /locus_tag="P5335_05830"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HRZ74429.1"
                     /translation="MEETGKRINKFLSESGFCSRREADRLIGESRVTINGIVPEMGTK
                     VFDTDEIRVDGKLIRENREKPIYLAFNKPAGIECTTNQSVRNNIVDYINYPERIFPIG
                     RLDKASEGLIFMTNDGDIVNKILRARNNHEKEYIVTVDKAITDNFIKKMSNGVPILDT
                     VTRKCSVEQINTNVFKIVLTQGLNRQIRRMCEYLGYEVIALKRIRIINIQLDVQLGRY
                     RDLTEVEIQELNQLIEPSSKTEEASLPKVETRKIQPKRKF"
     gene            12365..12949
                     /locus_tag="P5335_05835"
     CDS             12365..12949
                     /locus_tag="P5335_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74430.1"
                     /translation="MKKLVFLLVCTSAFLFNSCSDSDSDTPQNTNSTSFVVDGETYEL
                     ATTQGIVNIVSPEPIEVNGSFYYRNTLIISGVKGLTDVATVTFDLFISESLSLAGEYQ
                     ISTDQDDENPVDLENILTNQNRACLGWTSLIAKTNIISQNSTSGNAPSGILKIVSNGG
                     NNYTIQYTGNFKKIDQVGNIQVTMNLRRGVSNGS"
     gene            complement(13014..13904)
                     /locus_tag="P5335_05840"
     CDS             complement(13014..13904)
                     /locus_tag="P5335_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HRZ74431.1"
                     /translation="MELLDGKKIAEDIKNEIAAEVLKMKANGEKVPHLAAIIVGNDGA
                     SLTYVGSKVKSCERVGFESTLIKMPSTTSETELLKKIKELNEDDNIDGFIVQLPLPKQ
                     IDTQHVIMSIDPSKDVDGFHPENFGKMALDMSTFIPATPFGILELLERYGVETQGKHT
                     VVIGRSHIVGRPMSILMGRKGFPGNSTVTLTHSYTKNIAQITTQADIIITALGVPNYL
                     KAEMIKDDAVVIDVGITRVLDETNEKGYRITGDVDFEHVSKKASYITPVPGGVGPMTI
                     AMLLKNTLLAREQKRMKSKK"
     gene            complement(14004..15341)
                     /gene="ffh"
                     /locus_tag="P5335_05845"
     CDS             complement(14004..15341)
                     /gene="ffh"
                     /locus_tag="P5335_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRZ74432.1"
                     /translation="MFSNLSDKLDKAFHILKGHGKITEVNVAETLKEVRRALLDADVN
                     FKIAKDFTTAVKEKAIGQNVLTSLQPGQLMVKLVKDELTELMGGSEVGINLSGNPSVI
                     LMSGLQGSGKTTFSGKLANFLKTKKGKKPLLVACDIYRPAAINQLHVVGEQIGVEVYS
                     ELENKNAVEIAQNAIKYAKSNGFNVVIVDTAGRLAIDEQMMNEIANVHQAIQPQETLF
                     VVDAMTGQDAVNTAKAFNDRLNFDGVILTKLDGDTRGGAAISIKSVVDKPIKFIGTGE
                     KMEAIDVFYPSRMADRILGMGDVISLVERAQEQYDEEEARKLQKKIAKNEFGFDDFLA
                     QIQQIKKMGNMKDLVGMIPGAGKALKDVEIEDDAFKHIEAIIHSMTPLERTKPVVIDG
                     KRKIRIAKGSGTKVEQVNQLMKQFDQMSKMMKMMQGGGGKNLARMMGGMKGMK"
BASE COUNT         5351 a         2582 c         2556 g         4960 t
ORIGIN      
        1 aaaaatgaaa ttggtcttaa tcacaattgg tcttcttgca cttgcttttg ctgggattgc
       61 tattaaaata tgggcaaaaa aagatggaaa attttccgga acttgtgcca gtcaaagtcc
      121 gtttttaaat aaagagggcg aaaattgtag cttttgcgga aaaacttccg aacaaatgaa
      181 agattgttct gaaccttcac attcttaatc cagaaccaaa caatgctcga cattttattt
      241 tatagtttaa ttggagtcgt aatcattcaa ttattctatt atattcttgt atttggtaat
      301 ttttcgttta caaaaagcca gaaaaacaat ccaaagcgaa ttccgatatc cgttatagtt
      361 tgtgctaaaa atgaagcgga taacattaaa aaatttgttc cccttttatt agaacaaaat
      421 tatcccgatt ttgaattagt tctaattgac gattcgtcta cagatgaaac gttagaatta
      481 tttgaacaat ttgaaaaaca ggattcccgg gtaaaattgg ttaaagtcaa aaacaacgaa
      541 gccttttggg gaaataaaaa gtttgcctta accctcggaa ttaaagcagc taagaacgaa
      601 tatcttttgt tcacggatgc tgattgttat ccaacttcaa aagaatggat taaggaaatg
      661 agttctaatt tcaccttaaa gaaaacaatt gtattaggat acggagccta tgaaaaaata
      721 cctgattcgt ttttaaataa gctgattcgt tttgaaacaa tgttaacggc aactcaattt
      781 tttggatggg caaaaatggg caaaccctac atgggtgtcg gacgaaattt agcctacaaa
      841 cgagaagaat tttttagagt aaacgggttt attgaccaca tgaaattgcg ttccggtgat
      901 gacgatttat ttatcaatca agctgctgaa aaagaaaata caacagtttg cttttcaaaa
      961 gaaagtttca cttattccat gccaaaaacg acttataaag agtggtttac acaaaaacga
     1021 agacatgttt caacggctac ctattacaaa atgtttgatc ggtttcaatt agggttgttt
     1081 tttctgacac aattattatt tttaatatta gccattcttt tattgtccat taattttcaa
     1141 tggatgtttg ttttgccagt tgtgttaatt cgttacacct ttgcttggat atcaatgggt
     1201 tttgctgctt ctaaactaaa cgaaaaagat gttatctatt ggtttccggt catggaaatt
     1261 gctcttattt tcactcaatt aaatgtgttt tttactaata ttttttcaaa acctgttcat
     1321 tggaaataaa aaaaatcata gaaaaagcca ttaatggtga ccaagttgcc tttacttcat
     1381 tattagacat gtattggaac gaagtctatg gttttatgct caagcgaaca gaaaacgaaa
     1441 ccgatgctga agatataacc atcgaaactt tttccaaagc atttgataaa attgccagtt
     1501 ataattcaga atttcaattt aacacttggt taattgcaat tgctaaaaat gtccacattg
     1561 acatgcttag aaaaaagaaa tctacgcttt ttgtggatat gaatgacgaa gaagaccatc
     1621 aggcttataa tgttgccgat actacaccat ctgctgaaga caagttgatt acagagcaaa
     1681 atttgtctcg tttattagct tacataaaag agttaaaacc ccattatcag gaagttattc
     1741 aacttcggta ctttcaggaa ttaagttatc aggaaatagc tgaacatttg gacgaacctc
     1801 taaacaatgt gaaaataaaa ttgcttcggg caaaaaaatt gttggcagaa attattttaa
     1861 aaagataatt caacaaagcc taaaaaaaca ttaaactgct aaaatcccta atctttttct
     1921 atctttgctt ttctaaaaat tgcactatga atactgtttt agaaaacgtt caaccaccaa
     1981 ttggcaagcc aaaatggtta cgtgttaaac tacctaccgg aaaaaaatat actgaactac
     2041 gcggtttagt ggataaatat agcctaaata cgatttgtac ttctggcagc tgcccaaata
     2101 tgggtgaatg ttggggagaa ggcactgcta ctttcatgat tttaggaaat atctgtacgc
     2161 gttcttgtgg tttttgcggt gtaaaaacag gtcgtccgga aacagtagat tgggatgagc
     2221 ctgaaaaagt ggcccgttct atcaaaatta tgaatattaa acatgctgtt attactagtg
     2281 tagacagaga tgatttgaaa gacatgggtt ccataatttg gttcgaaacg attcaagcca
     2341 ttcgacgcat gaatcctgaa acaacgttgg aaaccttgat tcctgatttt caagggaacg
     2401 aacgcaatat tgatagaatt attgaagcca atccggaagt tgtttctcat aatatagaaa
     2461 ccgtacgacg tttaactcgt gaagttcgta ttcaagctaa gtttgataga agcttagaag
     2521 tgttgcggta tttaaaagct caaggaatta agcgtacaaa atcaggtatc atgcttggtt
     2581 tgggtgaaac agaagaagaa gtaatggaaa caatgcgtga acttcgggca gtaaatgttg
     2641 atattgtaac cattggtcaa tacctgcaac cgagtaaaaa acatttacag gtgaaagaat
     2701 ttatcacccc ggaacaattt aaaaaatacg aagactacgg aaaagaatta ggtttccgcc
     2761 atgtggaaag tggagcttta gttcgttcat cctatcatgc tcagaaacat attctttagt
     2821 ttcattctaa tttcatcatg aaaaaacgaa ttgcaatcaa tggttttggt cgaatcggac
     2881 gaaatttatt ccgcttatta ttaaatcatc caaccattga agtcgttgct atcaatgatt
     2941 tagccgatac caaaacaatg gctcatttat taaaatacga tagtattcac ggtattttaa
     3001 atgctactgt ttcgtttgat caagaaaata tcattataga tgaaaagaaa attcgctttt
     3061 acagagaaaa agatattcaa aacctacatt gggaagctat agactttgtt attgaagcaa
     3121 ccggaaagtt taatacgttt gatgaagcct atttacatat taatgcaggt gcaaaaaaag
     3181 taattctttc cgcaccaccg gaagatgatc taataaaaac ggttgtttta ggtgtaaatg
     3241 aatccatttt agacggttca gaacttattg tttcaaatgc cagttgtact acaaacaacg
     3301 ctgctcctat gattaaaatc atagacgagt tatgtggtat cgaacaagct tatatcacta
     3361 ctgtccattc ctacaccacc gatcaaagtt tacatgatca accacataaa gacttgagaa
     3421 gagctcgtgg tgcctcacaa tcaattgtgc ccacaacaac tggagctgca aaagctttaa
     3481 ctaaaatttt tccaaactta gtgaataaat taggaggaag tgggattcga gttccagttc
     3541 cggatggttc tttgactgac attacttgtt atgtttccaa agcgataact attgaagaaa
     3601 ttaacacagc ttttttaatg gcttctcaaa ataacttgaa aggaatactt gcttacactg
     3661 aagacccaat tgtttccgtt gacatcttag gaaaccaaaa ttcttgttta tttgatgctc
     3721 aattgacttc tgttattggc aaaatggtaa aagtagtagg ttggtatgac aatgaaattg
     3781 gatattcatc gagaattatt gacttaattg aatatatttc aaataaatag ttaattttat
     3841 gccatcaaat ttttcgattt tttggtatga agtattttat tttaaccatc cttttcttta
     3901 ctacagcaat aaatgctcaa aaaaaaacta acgctaaagt agcattagac agcactaatt
     3961 attatataga gttagccaat tttcataaaa aaaataataa tttcaggagt tcgcaagatt
     4021 atattcaaaa agccattgaa tttgctgaaa ttaaaaaaga tattcaagcc caagcagatg
     4081 cttattcttt tctaggcagt gtttatttcg aattaaaaaa aatcgaagat gctattcaaa
     4141 cttatgacaa aagcatcaaa ctgtatagca cacaaaaacc ctcctctaat caagcctatg
     4201 cattatataa tttaggtgtt tgttatgttg aaatgaaaga ttattctaaa gcagaatttt
     4261 attttaaagg agccgaaaat atttatgaaa ttattgaaat accggaagca aaagaaatgg
     4321 tcaaccttca aaagggaatt gtttacagag caaaaggtga aaatcaattg gctttatcta
     4381 ttttaaacac cattatcatt aaaccggatg aagaagacca ttttaaaacc aaagcggaag
     4441 cactttacca aattggacta attgaagctg aaaataaccg aaacaatcta gccataaatt
     4501 acttaacgaa agcgatggaa ttaagtacaa aaaataaaaa ttttgaactt aaatccaaag
     4561 tagcaaaatc attaagcgaa gtttatgaaa aaatgattga tataaacaat tcgttcaaat
     4621 atattaaaca acacatacga attagggata gtattaaaga aataatcaat aaaagagttg
     4681 gaacggaaga tttcttgagc tttaaacaag cagagcaatt aaaatctatc gaccaaatga
     4741 atcgcgaaaa taaagaacaa caaagagcta ataaattctc taaattaata agcatcttaa
     4801 gcatcgcatt aatctcaatt ttatctttat taagtttgtc tttgtataaa aataatatta
     4861 tccgaagtca atcaaacaag caattactag acaaaaatca agaattacaa attgctaaag
     4921 acaaggctga aaaagcctca aaagccaggg cagaattttt atctactgta agtcacgagt
     4981 tacgaacacc attgaatgcc atcaatggga ttacccattt attaattgaa gaaaatccaa
     5041 aaaaatcaca attacaatac ctaacttctc ttaaattttc cggtgattat ttactcactt
     5101 ttatcaatga tattttagaa ataaaccgcg ttgaaagtga aaacattgaa attgaaagta
     5161 tcaatgtaaa tgtcaaacaa ttattaagtg atttacataa ttcatttaaa gatttagcca
     5221 gtaaaaataa caatgaattt attttagaaa ttgacgaaaa aattcctgac catttaattg
     5281 gagatccaac aaagctttca caaatattca ttaatctcat caacaatgct ttaaaattta
     5341 ctaaaaacgg aaaagttagt attggagcat ctgttgtaaa aactgagaat gatatttcaa
     5401 ccgtacattt tgaaattgct gataccggta ttggaattcc aattgaaaaa caaagttcta
     5461 tttttgaaag tttttcacaa ggttctgttg aaataaatag aaaatatggt ggaaccggtt
     5521 taggtttagc cattgtaaaa agattagtta gtttacttgg cggcgaaatt caattagtga
     5581 gcagtcaagg tataggatcc actttctctt ttgacattga ttttaaatta ggtcaacaaa
     5641 catttgtaga agttaaaaaa cagtatgacg aaagcatttt tgtgcacaaa aaagttttgg
     5701 tagtagaaga caataaaatc aaccaaatga ttaccaagaa aatgttggaa aataaggaaa
     5761 tcatttgtga gttagttgac aacggtgaag aagctatcga gatggttaga agcaacgaat
     5821 atgatttaat actcatggat gttcacctac caggaattaa tggaacggag gccactgaaa
     5881 agattcgtgt attcaataaa acgattccgg taattgcctt aaccgctatt tctttaaatg
     5941 aaaatagaga aatgttactt tcgtttggga tgaatgatgt tgtaactaaa ccattcaatc
     6001 ctgataattt ttacaaagtt attgccaatc agcttagttc gatttaaaaa taaagccatc
     6061 tttcattacg aaaattacat tttccaatgt tttgatattt agcaatgggt tttcatctac
     6121 tgcaataata tctgctaaga agctttcttt gagttgcccc aaactgtttt ccattcccaa
     6181 taatttagcg ttagttatag ttgcagcaag aatagcttcc tttgcgggca ttcctgcttc
     6241 aaccatatag ccaaactctt tggcatttaa accatgaggg aaaacgcctg catctgttcc
     6301 aaaagcaatt ttcactcctc tattatatgc ttttgcaaat gtgttttgaa tttgaggtcc
     6361 tacttctaaa gcttttggtc taacaatttc aggataaaaa ccggttattt tggctttttc
     6421 agctacctct tttccagcag taatagtagg cactaaatat gcatcatact ttttcatcat
     6481 ttccattgtt tcctcactca taaaagtacc gtgctcaata gttttaattc ctcctaaaat
     6541 tgccctttgc attccttcat ctccatgtgc atgagcagcc gttaacatat tataatcttt
     6601 ggcagtagct gtaatagctt taatttcatc aagggtgaac tgaggatttt tcccgttttt
     6661 tgccacgctc aaaacacctc ccgttgctgt tatctttatt acatctgccc cttctttgta
     6721 tcgttgtcta acagctttta cagcgtcttc cggtgaatta attactcctt caaccggacc
     6781 cgggtcgccc attaactttc gagaacttcc attagttgga tcagcatgac ctcccgtagt
     6841 cgcaatcgat tttcccgcag caaatatgcg tgggccaact acaatacctt tatcaacagc
     6901 tttgcgaaga gaaatattta ctcctgaacc acctaaatca cgtacagtgg taaatccagc
     6961 cattaatgtt attcgagcat aacgggttga agcaaaagca acatcagcct cattatcagt
     7021 atatttagca tcatagttag tagagttaaa ctcattttca agatgcacat gcatatctat
     7081 aagtccgggt agcacaaatt tattctttaa atcaacttct atatcattcg gattaattgg
     7141 agcaacatac ccatttacga ctttaataat cttattgccc gaaacaacaa ttgtcatatc
     7201 agtgagtatt tttcctgatt gtgcatcaac tatttttcca caatgaagat atgtatcttg
     7261 tgcaaataaa atgcatgaga ataggaatgt aaaaagaaaa gcgaagttgt ttttcatact
     7321 attattttta gaaggccttt caaaaataca aatcttatcg attgaaagac aattgtttta
     7381 aataataatt gaattggatt catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnaaagccc ctttatgacc ttgaacggta gaaaccggaa aatttggaat ttcagcatag
     7561 ggaatcgtaa attcaatttc tatatcttgg gtaaaagaac caagtcctga acctagtata
     7621 actccaaatt caggagtata attagtttta ttctttaaat agttaacggt ttcttgaact
     7681 ttgtcccaca taatcgatta tttttttatt gaaaacatca ctattagata aaaaagaact
     7741 ttgaataggc aaaaaatata actggttttt aggcaatttc ccttttaaac gcacatagtc
     7801 tttttcagtt gtaatgatag gtttcttttt agctttattt ataattgttt gaatatctgt
     7861 ctctgaaaaa tcatggtgat caggataata gagcacctcg tcattcggag tctgcaaata
     7921 tgcaaaaaag ggctcgggtt tcgcaattcc tgccacaagt aatttatcaa cagttcgaaa
     7981 agaatcgaca gaacgagaat ctatttctga gcttaagctt tcatcataag taattgtaga
     8041 aaaataaaca gactgatttt cagaaacttt taatttattc aaacatttat tttgttcaaa
     8101 ttcagataaa tttggcggac attttgtcac tacaattata ttggctcttt ttgctccatt
     8161 tctactttcc cgaagatttc ctgtaggtaa aataaaatct tcagaataca aatctgaata
     8221 agcagtcaat aaaatataaa aaccggcttt tactttacgg tgttgaaaag catcatctaa
     8281 taaaattaac tccggtttct tttttaaaga aagtaatttt tcaattccgt tttttcgatt
     8341 agcatcaacc gccacttgaa tagctggaaa cttttgatgc atttgaaaag gttcatcccc
     8401 taaactttca gacgttgatg ttgcatctgc caacaaaaaa cctttcgatt ttcgtttata
     8461 tccacgactt aaaactgcaa tttgatagtt tgaagaaagc aaacgaatta aatattcaat
     8521 ttgtggtgtt ttaccggttc ctccaacgct tagatttcca acagcaatga tagggatatc
     8581 aaaggaataa gattttaaaa ttccgatatc gtagcaccaa ttacgaatag aagtaatgaa
     8641 tccatataaa atggcaaaag gaaagagtat ttttcgcaac aaaatcatgt tacgaaagta
     8701 gggatttttt atctttgaga gaaataatct caatttggat ttttagccaa ctcaattatg
     8761 aaactcaaca ctattataga acagttagaa atgcttgcac ctctttctta tgctgaagat
     8821 tttgacaatg taggtttgct taccggaaat agtgaacagg aaattaacgg aatcctagtt
     8881 tgtcacgacg tcttagaaaa tgttctaaac gaggctatag aaaaaaaatg caaccttatt
     8941 gtatgcttcc atccaattat tttttcgggt ttgaaaaaaa ttaccggaaa aacttatgtt
     9001 gaacgagtag ttattaaagc gattaaaaat gatattgcaa tttatgcctt gcataccgct
     9061 ttagacaatc atcaaaatgg cgtgaataaa attatttgca accaattagg attgaaaaat
     9121 accaaaatat tgattccaaa agagaaattc attcgaaaat tagtcaccta tactattttg
     9181 gaaaatcatg aagaactccg aaatgcactt tttgatgccg gagcaggaaa tattggaaat
     9241 tatgaagatt gcagttttaa ttcgaaagga attggaacgt atttaggaaa tgagaacagt
     9301 aatcctgaaa ttggagaacg atttgaattt gtagaaaacg aagaaataaa attagaagtc
     9361 acttttgaaa aacagttaga atcgaaaatt ttgaaagcgt tgttcaaaac acattgctat
     9421 gaagaagtgg cttatgaaat ttatgaatta caaaatcagc atcaaaatat tggtttagga
     9481 atgattggtg aattagaaac cgaactttca gaattggatt ttcttcaatt tgtaaaagat
     9541 aaaatgcaat gtggaagtat tcgacacagt gaatttttac ataaaaacat taaaaaagtg
     9601 gcggttttag gcggttcagg tagttttgcc ataagtcagg caaaaaaaat gggagctgat
     9661 gcttttttaa cagctgattt gaaatatcac cagttctatg aggctgaaaa tcaattactt
     9721 ttagctgata ttgggcatta tgaaagcgaa cgatacacaa aaaattatat tgttgatttt
     9781 cttatcaaaa aaatccctaa ttttgccatc attttatcag aacaaaatac aaatccagtt
     9841 aagtactttt agattatgac aaacgtaaaa gaaatgagtg tagaagaaaa gttaagagca
     9901 ctctatgact tgcagttaat cgactcaaga attgatgaaa tccgaaatgt aagaggagaa
     9961 ttgccgcttg aagtgcaaga tttagaagat gaagtagcgg gtttgaaagc acgtaacgaa
    10021 aagttaaaag ccgatttaga gcaaattgat gatcaaatca aaggcaaaaa gaatgctatt
    10081 gaagctcaca aagaaggaat taagaaatat actgaacaac aaaaaaatgt tcgtaataat
    10141 agagaattca attctttaac aaaagaaatc gagtttcagg aactagaaat tcaattgagc
    10201 gagaagcaca ttaaagaaat gaaagcttct gttgaatata aaaaacaaca agtagctgat
    10261 tctcaagaac gtttaaatgc aaaacaatcg catttagaac acaaaaaagc ggagttgggt
    10321 gatatcatgg ccgaaacgca aaaagaagag gaatacttaa ccaagttatc aactgaatat
    10381 gcttcaaata ttgagcctcg tttgttagct gcttataacc gaatcagaaa tagtgttaga
    10441 aatggattag cggtagtttc catcgaaaga ggagcatctg ccggatcatt cttcaccatt
    10501 ccaccacaaa cgcaagttga aattgccgcc cgcaaaaaaa tcttaactga tgaacacagt
    10561 ggacgaattt tagttgacaa cgttttagct acagaagaga aagaaaaaat ggaaagcatt
    10621 tttgctaaga tttaaaaata caaaccccgg attcggggtt tttttatgaa taaaacgctc
    10681 aaaaatattg taacaaaatc aattggtgcc tacatcaatc tgttgagcca tacctttccg
    10741 aaaagtgcca cgaaattagc ctataagttt tttagtgagc ctcgagttgg aaaattaaat
    10801 gccaattcac ttcctaccat tttacaaaaa gcggaacgag aaatagtaca attggaaaat
    10861 tatcaatttc ctgtctatat ttggaaagga aatgaaacaa aaattctttt agttcacggt
    10921 tgggaaagta atgccgctcg ttgggaacca atttttccat ttcttcaaaa atcaggaagc
    10981 accattattg ctttggatgg acccgcacat ggattagcca gtggtgtaga atttagtgtg
    11041 ccaaaatatg ctgattttat tcatgaagta gttttaaaac ataaacctca aatcattatt
    11101 ggccattcta tcggtggctc agcttgctta tattttcagc atcattttac tacactttcc
    11161 ctagaaaaaa tggttttgat gggagcacct tccgatttag aagtattatt ggagaattat
    11221 gccaaattac ttgggctaaa tactaaagta atccaacttt tggatgccta tttcttaaaa
    11281 cgattcaata ttaagacgaa agaatttctg ggaaaaaaac tagcctccac cctatccttg
    11341 aacggaatta tttcgcatga tcgtgaggat gatgttgtgc tattttcaga aagcaaaaaa
    11401 attgcggaag gttgggaaaa agctactttt attgaaacaa aagggctagg acacagtatg
    11461 catgatgaaa aattgtatga atcaatttat tcatttctat ttcaaaagct ttagagtatc
    11521 tttgccctat ggaagaaacc ggaaaacgca tcaacaaatt tttatctgaa tcaggatttt
    11581 gttcccgtag agaagccgac agattaattg gagaaagtcg tgtaacaatc aacgggatag
    11641 ttccggaaat gggaaccaaa gtttttgata ccgatgaaat tcgcgtagat ggcaaactaa
    11701 ttcgagaaaa cagagaaaaa cctatttatt tagcctttaa taaacctgcc ggaattgaat
    11761 gtacaaccaa tcaaagcgtt cgtaataata ttgtggatta tattaattat ccggaacgaa
    11821 tttttccaat tggtcgatta gataaagcca gtgaaggttt gatttttatg acaaatgatg
    11881 gtgatattgt caataaaatt cttcgtgcta ggaataatca cgaaaaagaa tatattgtta
    11941 ctgttgacaa agctattact gacaatttta taaaaaaaat gagtaatggt gtgcccattt
    12001 tagacacagt tacaagaaaa tgtagtgttg aacaaatcaa tactaacgtt tttaaaattg
    12061 ttttgacgca aggtttaaac cgacaaattc gtaggatgtg tgaatattta ggatatgaag
    12121 taattgcact aaaacgcatt cgaattatca atattcaatt agatgttcag ttaggtcgtt
    12181 accgagattt aactgaggta gaaattcaag aattaaatca attgattgaa ccttccagta
    12241 aaacagaaga agctagcttg ccgaaggtgg aaacccgaaa aattcaaccg aaacgtaaat
    12301 tttgatccca cattttggtt attaaagata ttttctattt ttgtgaaaac attaaattta
    12361 aaatatgaaa aaactagtat ttttattggt ttgtacttct gctttccttt tcaacagttg
    12421 tagtgatagc gacagtgaca ctcctcaaaa cacaaactca acctcttttg tggtagatgg
    12481 tgaaacctat gaattagcta cgacgcaagg aattgtaaat attgtttctc cggaacctat
    12541 agaagtaaat ggtagttttt attatcgaaa cactttaatt attagtggag ttaaaggatt
    12601 aaccgatgta gcaacagtta catttgatct atttatatca gagtcattat ctttagccgg
    12661 cgaatatcaa atttcaaccg accaagacga cgaaaatccg gttgatttag agaacatttt
    12721 aaccaatcaa aacagagctt gtttgggttg gacttcttta attgccaaga caaacattat
    12781 atcacaaaac tcaacaagtg gtaatgcccc ttcgggtatt ttaaagattg tttcaaacgg
    12841 aggaaataat tataccattc aatataccgg taattttaag aaaattgatc aagtaggaaa
    12901 tattcaagta acgatgaatt taagaagagg cgtttcaaat ggtagttaat tcgcatataa
    12961 tagtgcaaaa aaaacaaaac ccaagcttat cacttgggtt ttgttttttc taattacttt
    13021 ttgcttttca ttcgtttttg ttctcttgct aataaagtgt tttttagcaa catggctata
    13081 gtcatcggcc caactccacc aggaacaggt gttatatagg aagccttttt actcacgtgt
    13141 tcaaaatcta catcacctgt aattctgtac cctttttcat tggtttcatc caaaactctg
    13201 gtaattccca catcgattac aacggcatca tctttaatca tttccgcttt taaataattt
    13261 ggcacaccca aagcggtgat aataatgtcc gcttgagtgg ttatttgagc aatattttta
    13321 gtgtaactat gtgtcaatgt aactgttgaa tttcccggaa aaccttttct tcccatcaaa
    13381 atactcattg gccttcccac aatatgactt ctaccaatca ctacagtatg ttttccttgt
    13441 gtttcaactc cgtaacgttc taataactcc agtattccaa aaggagtcgc cggaataaat
    13501 gtactcatat ccaacgccat ttttccaaaa ttctccggat gaaaaccgtc tacatcttta
    13561 ctaggatcga tagacataat tacatgctgc gtatctattt gttttggcaa aggcaattgc
    13621 acaataaaac cgtcaatatt atcgtcttcg ttaagttctt taattttttt aagcagttca
    13681 gtttcagaag tggtactagg cattttaatc aaagtacttt caaatccaac ccgttcacac
    13741 gattttactt tacttccaac ataggttaaa cttgctccgt cattaccaac aattattgct
    13801 gctaagtgag gaactttttc accattagct ttcattttta gaacttccgc agcaatttca
    13861 ttttttatgt cttctgctat tttttttcca tccagtagtt ccattttata tagtttagta
    13921 gttatgcatt tacttttgca ttataaaaag ttagttgtgt ttttataaaa aaataagacg
    13981 cgaaaaatcg cgtcttagta gtattatttc attcctttca ttccgcccat cattctggcg
    14041 agatttttac cgcctccgcc ttgcatcatt ttcatcattt tactcatttg atcaaattgt
    14101 ttcatgagtt ggtttacttg ctcaacttta gttcctgaac cttttgctat tctaattttt
    14161 cttttaccgt caatcactac cggtttcgtt ctttctaaag gagtcattga atggataatc
    14221 gcttcaatgt gtttgaaggc atcatcttca atctctacat ctttcaacgc tttaccggca
    14281 ccgggaatca taccaaccaa atctttcatg tttcccattt tcttaatttg ttgaatttga
    14341 gctaagaaat catcaaaacc aaattcattt tttgctattt tcttttgtaa ttttcgggct
    14401 tcttcttcgt catattgttc ttgagcacgt tcaactaatg aaattacgtc tcccattccc
    14461 aaaattctgt cagccatacg agaaggatag aaaacgtcaa tcgcttccat tttttctccc
    14521 gttccaataa atttaattgg tttgtctaca actgatttaa ttgaaattgc agctccacca
    14581 cgggtatccc catctaattt ggttaaaata acaccatcaa aattcagtct gtcattaaat
    14641 gcttttgcgg tattcacagc atcttgacct gtcatggcat cgactacaaa taaagtttcc
    14701 tgtggttgaa ttgcttgatg aacattagca atttcgttca tcatttgttc atcaatagcc
    14761 aagcgaccgg ccgtatcgac aatgacaaca ttaaatccgt ttgatttcgc atatttaata
    14821 gcattttgag ctatctctac cgcattttta ttttccagtt ctgaataaac ttctacccca
    14881 atttgttcac caacaacatg caactgatta attgccgccg gacggtaaat atcacaagct
    14941 actaaaagtg gttttttgcc ttttttagtt tttaaaaagt tggccaactt tcctgagaat
    15001 gtcgttttac ccgaaccttg taatcccgac atcaaaataa cggatggatt tccggaaagg
    15061 tttataccta cttcagagcc acccattaat tccgtcagtt catctttcac taacttcacc
    15121 attaattgac ccggttgcaa agacgttaaa acgttttgac caatggcttt ttctttgaca
    15181 gctgttgtaa aatctttagc gattttaaaa ttcacgtcgg catcaagtaa ggcacgacgc
    15241 acttctttta aagtttctgc aacgtttact tcggtaattt taccgtgacc ttttaatata
    15301 tggaaagctt tgtcgagttt atcgcttaaa ttactgaaca taaaatggat tttctaatga
    15361 tgaagtgcaa agataacaca atccaaataa gcatcaaaaa atgattttag caaattttta
    15421 aattttatgg tagggtttct tcaccacaaa ctgttttagt actataattt aaaacgtaat
    15481 aaacatgaaa acccaaaaaa tgttacttag tctactaata cttttagtat tgacaatctc
    15541 gtgcatcaa
//
LOCUS       DAOTCW010000051        15499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2199_length_15499_cov_42.003885, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000051 DAOTCW010000000
VERSION     DAOTCW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 15499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15499
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2199_length_15499_cov_42.003885"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1277
                     /locus_tag="P5335_05850"
     CDS             150..1277
                     /locus_tag="P5335_05850"
                     /inference="COORDINATES: protein motif:HMM:NF039990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HRZ74433.1"
                     /translation="MKQLKVCLLVLAVNFAPQKGSAQASPEEGTQFSLYGFGRTTLVW
                     DNQDLGRSDLFVPANIKVDTPKNPNFFIGAKQSRIGFDVKHKLGEDLMTIKIEGDFHN
                     DATDATGLFRMRHAYVNYKFALIGMTWSNFFDEEVNPVTVDFEGPNSSTLSRTPQVRF
                     STYKSKNVLSLSLENPMEKVTLGGNITALSERFPDVIGAYRINGSFGFIKAAALFREI
                     RYESDKARSLYGYGATVMTALKVGTKDKIKFQGVMGTGVSKYIQGASGLNYDAVYNGT
                     NELEALQMTGLNLTYQHFWKDHIYSSLTGGFLNVEDNDNLTPSDYQSGYYGSLNLFWD
                     AVKNLTFGVEALAGERINVDDSSGTAFRFQMNATYKFNKLF"
     gene            1300..2103
                     /locus_tag="P5335_05855"
     CDS             1300..2103
                     /locus_tag="P5335_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HRZ74434.1"
                     /translation="MNLNMKTLLFGFSFLLLSCGQNKPTEPTAPENENEVNATAPDAE
                     AKKTQGYVLPGLDHGFEQSPVNIISSANKDGKHDITLYFKDEINKIENLGHTVQLDFA
                     EGSTITQDDTIFAFKQCHFHTPSEHLIDGMTYPMEMHIVNIMPNEDKTATPQYLVIGV
                     LFKEGKENKFIADFLNAIPKDEKGTADLKAGKVTLHDLFGAIPKNLKGAFYNYRGSLT
                     TPPYTETVRWYITKHVFEASAAQIEAINKIEGNNARHVQALYGRKVSSN"
     gene            complement(2210..3742)
                     /locus_tag="P5335_05860"
     CDS             complement(2210..3742)
                     /locus_tag="P5335_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRZ74435.1"
                     /translation="MTIEKLTKELEELLQYIIDNEAKIDETLSGIHSNYRLSAKNLCR
                     YLLLRSFDLRKYHDSLSDCGVSSLGTSEGYVYSNLYNVVKNLKLIQGLPIEEHSAIEI
                     IGYEKSKKLIKKNARKLFKAVHKNHFIEIMVTLPNEAAEDKSIIEKMVESGMEIARIN
                     LSHGNIGIWTKMVQFIHEINRETNQEIVIYMDLSGPKLRTAPFKIGKKKHKFKQDIPV
                     CVGEHLLLTKRATHGKKSKFGKKNKQLKKGEIGVMLPEIIDDVQVNDRVLFDDGMIES
                     IVIAKTSENVELLITHCYKSKLASEKGINLPDTKLNLPALTERDIENLPFICQNADIV
                     GYSFVRTAADVQYLYHQLQMNQANELGVVFKIENKEAFENLPFILLEGMKRDKIGVMI
                     ARGDLAVEIGFERSSEVQNEILWICEAAHIPVIWATQVLENLAKTGIPTRAEISDVVQ
                     GVHAECIMLNKGPHINEAIVLLKNILVRMEAHSFKKKNSLRALNIAKNALDQLMGEEI
                     LT"
     assembly_gap    3780..3879
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3880..4460)
                     /locus_tag="P5335_05865"
     CDS             complement(<3880..4460)
                     /locus_tag="P5335_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009089634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HRZ74436.1"
                     /translation="MSFIFKSVDTVETISREDFKKNYLDPRKPLIIKNLSKDWPAREK
                     WTLDYFKEIAGDLQVDLVDNSKADPSKAINAKLASMKFGDYLDLIKREPTELRIFFLN
                     LFKHSPELLNDIKIPKELMGGFIESMPAMFFGGTKAITFLHYDIDLPHLFHTHFGGRK
                     HIILFDNKWKKRLYCIPNTTYALEDYDVANPDF"
     gene            4662..5114
                     /locus_tag="P5335_05870"
     CDS             4662..5114
                     /locus_tag="P5335_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HRZ74437.1"
                     /translation="MNSKKIAEYRKLLEVTKTATLKELKTVYRNSMKDSHPDTIADAV
                     ERHAAEEKSKAVIEAYHFLVSIAPETQEKVKEEYVKTTTTSGIMDFYFENGVLYINFL
                     DGNTFEYFGVPKVTYIKLVNAESPSRFARRHIYNEFLYRSASKLVAAE"
     gene            5355..6092
                     /locus_tag="P5335_05875"
     CDS             5355..6092
                     /locus_tag="P5335_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRZ74438.1"
                     /translation="MKKFIFILFFSLFTINTLESQVLISLLFGDNLNTGKIEFGLDGG
                     MTLSDQTGLSDSKMLNSWNLGFYFDIKLKDDWMLHTGVLVKSTSGADEIPVYSLQNSE
                     LDNLYATGSIERNMSYFNVPILMKYNFKNRFYVEAGPQLGLLYKATDEFQNKIDGNEL
                     TYSKDVREEFHPLDAGVVGGVGYRILKGYGMNIAIRYYYGFVDVLVNDSGDAVTNQSL
                     YFNVGIPIGVGKALKRAEEKKKAEQKQ"
     gene            6113..>7197
                     /locus_tag="P5335_05880"
     CDS             6113..>7197
                     /locus_tag="P5335_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74439.1"
                     /translation="MKPNSFNPISFVVLLIVVLFAQNSFAQVYSNKEVGKKNQEIIDS
                     LKTAEYPYSLPIWGAKATEKGFSLPYSAGIGVNYLGQESDLIIENLMVGFNNGPLYDL
                     DEIVRFNGATSTASGINVRPDIWLFPFLNVYGILAKSNLSTAIDAGLWVPNADGVWSE
                     VIDFSTKADFQATTFGIGFTPTIGVAGGWIALDMNVTWSDVEALEKPARAFVFGPRFG
                     KTFKLNKPEQNLTFWTGAFRLKLNSETSGSLPLSDILSTDGLQTKVDNGIIKVETTQL
                     QVDDWWNNLTNVQQNNPVNIAKYETANRALETVGGFLNSIDSALNDENTATVQYSLDK
                     RPADMWNFLLGTQFQYNKHWMVRAEVG"
     assembly_gap    7198..7297
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7298..7719)
                     /locus_tag="P5335_05885"
     CDS             complement(<7298..7719)
                     /locus_tag="P5335_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRZ74440.1"
                     /translation="MAEYIKDYSIFYEFIKKFMPNGFKEVNENDPVFLELNELLEANN
                     QFFYIADIIQLKILWTSRQSKKIIGIEPEDVSPYFFMEATHPDDIQRLNLGKTRLIKL
                     SQDMFIAGEGKALLSTNFNVRNATGGYTNLFMQNYLFYT"
     gene            8166..9050
                     /gene="era"
                     /locus_tag="P5335_05890"
     CDS             8166..9050
                     /gene="era"
                     /locus_tag="P5335_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRZ74441.1"
                     /translation="MTHKAGFVNIIGNPNVGKSTLMNAFIGEKLSIITSKAQTTRHRI
                     LGIVNGEDFQVVLSDTPGIIKPAYEMQESMMQFVKSAFEDADVLLYMVEIGEKELKDE
                     AFFNKIIHSNIPVLLLLNKIDKSNQEQLEEQVAMWQEKVPNAEIMAISALENFNVKEV
                     FNRILELLPESPPYYPKDMLTDKPERFFVNETIREKILLNYDKEIPYAVEVETEEFIE
                     DEKIIRIRSIIMVERDTQKGIIIGHKGEALKRVGMQSRHDLEKFFGKQIHIELYVKVS
                     KDWRSNAFQLRRLGYNQK"
     gene            9162..10172
                     /locus_tag="P5335_05895"
     CDS             9162..10172
                     /locus_tag="P5335_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HRZ74442.1"
                     /translation="MKIFVIFFFSLFNVTLYAQKDTIIFKNKDVIVGELKQLNNNILT
                     VKTSYSDKDFAIEFDKVVSLVLENKYSVVLSNGFRMYGYVKTENPNKAVITQENNVAI
                     EVFLNEIVVLRKIDDGFWNHFKGSFDFGYNLTQTNNSEQFTFAGLLNYYSERWIHRAT
                     YNQLFTSQDDVEDIERTDWSIDTKRYLKRKWFLNTNISFLSNTSQSLKGRFVPSVGAG
                     NYLVRSNKLYLLVGTGLTYNIEKYEDASTDKTSTEALLITQFNMFNFKDIDLYISAVG
                     YPSLSESGRFRTDANVRFKYDLPFDFYFKTELQVNFDNQPAAGAVKTDYVFSTGFGWE
                     IK"
     gene            complement(10176..>10568)
                     /locus_tag="P5335_05900"
     CDS             complement(10176..>10568)
                     /locus_tag="P5335_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74443.1"
                     /translation="VEPDYKIKISGHHIWLHIGMLNREKHSPHLHLEIEKMEDGNTAV
                     RGLYGPDPVLWTMFMFLHFVVAGIFVIFSMIAFSKWSLDQPFGFDLLVMFAMVNSWFL
                     LYFIARLNRKKGINQAKELEVLMERILE"
     assembly_gap    10569..10668
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10669..11346
                     /locus_tag="P5335_05905"
     CDS             <10669..11346
                     /locus_tag="P5335_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRZ74444.1"
                     /translation="RDSIDTKYDRFGFEFNLVDTAGIRRKAKVKEDLEFYSVMRSVRA
                     IEHSDVCILMVDATRGFEGQDQSIFWLAAKNRKGIIILVNKWDLVEKDTMSTRDYEAK
                     IRKELEPFTDVTILFVSALTKQRLLKALEETVKVYENRKQRIPTSKFNDFMLKLIEAY
                     PPPALKGKYVKIKYCMQLPTPTPQFVFFANLPQYVKEAYKRFLENKIRENWDFSGVPI
                     DIYIREK"
     gene            11430..11864
                     /locus_tag="P5335_05910"
     CDS             11430..11864
                     /locus_tag="P5335_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74445.1"
                     /translation="MRNLISTVFLFIAGFTTLNAQEVGIRFGEMTGNNVAVDGVFSLG
                     EFSRIHADISFGDGLGVDALWDFIYKPIDDDFNWYVGFGPSVLFDDPFFLGVSGEIGI
                     EYHFDTVPIAIGLDYRPTFWIIEETDFDAGGFGLNIRYVFDK"
     gene            12018..12605
                     /locus_tag="P5335_05915"
     CDS             12018..12605
                     /locus_tag="P5335_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HRZ74446.1"
                     /translation="MLKSVIRIGIISLLFFLTVCCGSTISVTSDYDKGVDFKKYKTFS
                     FYHLKTTGEVSQVNADRLVKAIVLEMQGKGFVESSDNPDLLINAVTVITEKEAVSVSS
                     EYYGYGGYYRPYAYGYGYGYGTGYPVENTAAVNSYNYRDGTIMIDVLEAKSEKLIWEG
                     TGTAEINTKPSDPAKVIGYAVSKIMESFPPEPKKK"
     gene            12632..13360
                     /locus_tag="P5335_05920"
     CDS             12632..13360
                     /locus_tag="P5335_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HRZ74447.1"
                     /translation="MRNLVVGIFASMMLFSGNAQVKKPDTIAVAIFDRMSDVIGEMSS
                     ISFTLQIRDDEMNQDLGMITRFTESRVNFNGPNQMQIHSYGDKGHRGFWFSGESLVCY
                     SFEENNYTIIDSPSNTLDMINEVNDKYGIDFPAADFFSPTFTDDVLSNFDELKYVGMR
                     TISNKECFYIIAKRDDMIVQLWIANDALNLPVKMLIMEIGGKVTEQYEVTFSNWDINP
                     FLPDAMFKFQTPPKAKKISILPRK"
     gene            13387..14631
                     /locus_tag="P5335_05925"
     CDS             13387..14631
                     /locus_tag="P5335_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HRZ74448.1"
                     /translation="MKSTNYTSYLSLISVFFTLLFLNVYTTEAQRMGRGGGGGGGRAS
                     SSMSRPSSSYGGGSASTRQSSSYGGSSASTRQSSSYGGGSASTRQSSSYGGSSSASNR
                     QSINGGSYKSPDRSINNPSAGNSSMNSAGNRAGERPSKPSTNEATSGRNKGTSNDKLS
                     SSSKPGVSTNDRTKPSTGSGDRNKPSAGTSDRTKDNVNGGRGNGNNNNNNNINGGRGN
                     GNTNINIQNNYYGRPNTMPYYRPPYAYGGYHYYCYHPYYYHPYTPYYYGPYYNPWGVF
                     VATIAVTAIVVNSQSQQYYYDNGVYYVQSDGGYTVVEAPAGVSVKALPKEAQTVVVNE
                     TTNNYYYAGTYYEKKGDEYVVVPPTAGTVVENLPKGGEEVKIGEVTYVQVGKTYYQPI
                     KKDGKDMYEVVNVEKEQESKQK"
     gene            14906..15373
                     /locus_tag="P5335_05930"
     CDS             14906..15373
                     /locus_tag="P5335_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74449.1"
                     /translation="MKKMMVIALLAIFTMSTYAQQSKDEIAILQSAYGMQKRELVSKY
                     MNISEAQSATFWKLYEEYEVARKAIGTKRANNILEYAKKYDGITDENVQSLVKTSFEV
                     NKEFNALWEKTYKQMAKSLSPTTAAKFYQLELYLETMVRNELSESIPMIDELK"
BASE COUNT         4944 a         2595 c         2854 g         4806 t
ORIGIN      
        1 catttctaaa ataagtatta attttataaa atatatcgat cattagtaaa accaaacaac
       61 atgaaatata tcatcaattc ttcctgatgc tgcggaatat tccgtaattc ttttttttat
      121 caatttctaa ttttaagcta ctgaggctca tgaaacaatt aaaagtatgt ttactcgtgt
      181 tggcagttaa ttttgcccca caaaaaggtt ccgctcaggc atcccctgaa gaaggaaccc
      241 aattctcact ttatggattc ggtcgaacaa ccctggtttg ggataaccaa gatttgggaa
      301 gaagtgattt gtttgttccc gctaacatca aagtggatac gcctaaaaac cccaactttt
      361 ttatcggtgc taagcaatcc cgcattggct ttgatgtcaa acacaagctg ggagaagacc
      421 tgatgacgat taaaatagaa ggcgacttcc acaacgatgc caccgatgcc accggtctgt
      481 ttcgtatgcg acatgcttac gtaaactaca agtttgcatt gattggaatg acctggagta
      541 acttttttga cgaagaagtc aatccggtaa cagtcgactt tgaagggcct aacagttcaa
      601 cattgtctcg aaccccacaa gttcgttttt ctacctataa gtctaaaaac gtactctcct
      661 tgtctttgga aaatcctatg gaaaaagtca cgttaggagg caatatcact gcgttatcag
      721 aacgtttccc ggatgttatc ggtgcctatc gcatcaacgg ttcttttggt tttataaaag
      781 ccgctgcttt gttcagagaa attcgctacg aaagtgacaa agcccgaagt ctttatggct
      841 atggggcaac agtcatgacc gctcttaaag taggaaccaa agataaaatc aagtttcaag
      901 gtgtaatggg cacaggagta tccaaataca ttcaaggagc cagcggatta aactatgatg
      961 cggtatacaa cggaacaaac gaattagaag cattacaaat gaccggttta aatttaactt
     1021 atcaacactt ttggaaagac cacatctatt cctcccttac cggtgggttt ttaaatgtag
     1081 aagataacga caatttaacc ccgagtgatt accaatccgg ttactacggt tctctaaatt
     1141 tattttggga tgcagtaaaa aacttaacct ttggcgttga agccttggcg ggtgaacgaa
     1201 tcaatgtaga cgacagttcc ggcacagcat ttcgctttca aatgaatgcc acctataaat
     1261 ttaataaatt attttaataa acctttaaaa aacaaaccca tgaatttaaa catgaaaaca
     1321 ctcctttttg gcttctcctt tctgctgcta agttgcggac agaataaacc aacagagccg
     1381 actgcccctg aaaatgaaaa tgaagtaaac gcgactgctc ctgatgcgga agcaaaaaag
     1441 acgcaaggat atgtcctacc gggactcgat cacggttttg aacaatcgcc tgtaaatatt
     1501 atatcttcag ctaacaagga tgggaagcac gacatcacct tatattttaa agatgaaatc
     1561 aataaaatcg aaaaccttgg acacacggtg cagttagatt ttgcagaagg aagtaccatt
     1621 acacaagatg atactatttt tgctttcaaa caatgccatt ttcacacacc ctctgagcat
     1681 ttaattgacg gaatgaccta tccgatggaa atgcacattg taaacatcat gccaaacgaa
     1741 gacaaaacgg caacaccaca atatcttgtg ataggtgttc tgtttaaaga aggaaaagaa
     1801 aataaattca tagcagattt tttaaatgca attccaaaag atgaaaaagg cacagctgat
     1861 ttaaaagccg gaaaagtaac attgcatgac ttgtttgggg ctattccaaa aaacctgaaa
     1921 ggagcttttt acaattaccg cggttcttta actactcctc cctatacaga aacagtgcga
     1981 tggtatatca caaaacatgt ttttgaagcc tctgccgcac aaatagaagc tatcaataag
     2041 attgaaggca acaatgcacg acatgtacaa gccttatacg gaagaaaagt ttcttctaat
     2101 taattgattg ctcaataaag caagcacaga gccggttgaa aataatcaac cggctctgtt
     2161 catttgtttc cacttcgaag attcataaca aataggaaag gggatgtgtt taagtcaata
     2221 tttcttcacc catcagttga tccaatgcgt ttttggcaat gtttaacgct cgcaacgaat
     2281 tctttttctt aaaggaatga gcttccatcc gaaccaatat gtttttcaac aatacaatgg
     2341 cctcattaat atgcgggcct ttgttaagca tgatgcattc agcgtgcacg ccttgcacca
     2401 catcactaat ttctgctcgg gttggaattc cggttttagc aaggttttcc aacacttgag
     2461 tagcccaaat aacgggaata tgagctgcct cacaaatcca taagatttca ttttgcactt
     2521 ctgaacttct ttcaaaccca atttcaaccg ctaaatcgcc tcgggcaatc atcaccccaa
     2581 tcttgtcgcg tttcattccc tcgagtaata taaagggaag gttttcaaag gcttccttgt
     2641 tttctatttt aaaaacaaca cccagctcat tcgcttgatt catttgtaat tgatgataca
     2701 aatactgcac atcggctgcg gttctcacaa aagagtaccc cacgatgtct gcattttgac
     2761 aaataaaagg aaggttttca atgtcccgtt ccgttaaagc cggaaggttc agtttagtgt
     2821 caggcaggtt gatgcctttt tcagaggcta atttcgattt gtaacaatgc gtaatcaaca
     2881 gttcgacgtt ttccgatgtt ttagcaataa cgatggattc aatcatccca tcatcaaaca
     2941 gcacccgatc attcacttga acatcgtcaa tgatttcggg gagcatcaca ccgatttctc
     3001 cttttttcag ttgcttattc ttctttccga attttgattt tttgccatgg gtagctcgct
     3061 tggttaacaa gaggtgttcg cctacacaaa ccggtatatc ttgtttaaat ttgtgtttct
     3121 ttttgcctat tttaaaggga gccgttcgta gtttcggtcc cgataaatcc atgtagatta
     3181 cgatttcctg attggtttcc cgattaatct cgtgaataaa ttgcaccatt tttgtccaaa
     3241 taccgatgtt gccatggctt aaattgattc gggcaatttc cattccgctt tccaccatct
     3301 tttcaataat cgatttgtct tcggcggctt cattgggaag tgtaaccatg atttcgatga
     3361 aatgattttt atggacggct ttaaaaagtt ttcgggcgtt tttcttaatt agttttttac
     3421 ttttttcata cccgatgatt tcgatggcag agtgctcttc aataggcagt ccttgaatca
     3481 attttaagtt cttcaccaca ttgtaaaggt tgctgtacac atatccctca gaggtgccca
     3541 acgaggaaac gccgcaatcc gataaactat cgtggtattt tcgtaaatcg aaacttctga
     3601 gtagtaaata gcggcataaa ttctttgcac ttaatcggta gttcgaatga attccggaga
     3661 gggtttcatc aattttagct tcattgtcaa tgatgtactg caaaagctcc tctaattctt
     3721 tcgtcaattt ttcaattgtc atagtgcata catttattct tttggcttac tcgtttgtcn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt caaaatcagg attggctaca
     3901 tcataatcct ctaaggcata cgttgtattc ggaatgcaat acaatcgctt tttccattta
     3961 ttgtcaaaaa ggataatgtg ttttctgccg ccaaaatgcg tgtgaaacaa atggggcaaa
     4021 tctatatcgt aatgtaaaaa agtgattgct ttggtaccgc caaaaaacat ggcaggcatg
     4081 ctttcaataa aaccacccat caactctttt ggaattttga tgtcatttaa taattccgga
     4141 gaatgcttaa ataaattcag gaaaaagatg cgaagttcgg taggttcccg tttgattaaa
     4201 tctaaatagt caccaaattt catgctggcc agtttggcat taattgcttt ggaaggatct
     4261 gctttagaat tatccaccaa atcaacttga agatcacccg caatttcttt aaaataatcc
     4321 aacgtccatt tctctcttgc aggccaatct ttagataagt ttttaataat taacggtttt
     4381 ctgggatcta agtagttctt cttgaaatct tcacgactta ttgtttcaac agtatcaaca
     4441 gatttaaaaa taaagctcat agttgtatta agtataaaaa cagaaggata ttcacattaa
     4501 caagacggta aaattatcaa tttaaagtat tcaatttctt aattttgata taaattttgt
     4561 tccaaaagac agaagatcac acaagttagg attgaattaa aaaaacaact acttttgcaa
     4621 aaaattaaaa acgtggggaa gcacgtttgt aaagtcaact tatgaattca aaaaaaattg
     4681 cagaataccg aaaactgctt gaagtaacaa aaacagccac gctgaaagaa ctgaaaacag
     4741 tttacagaaa cagcatgaaa gacagtcatc ctgacaccat tgcagatgcc gtggagcgac
     4801 atgccgcaga ggaaaaaagt aaagccgtca ttgaagctta ccatttttta gtgagtattg
     4861 ctccggaaac gcaggaaaaa gtaaaagagg agtatgtaaa aacgacgacg acttcgggaa
     4921 taatggattt ctattttgag aatggggtat tgtacattaa ctttttagac ggaaacacgt
     4981 ttgaatattt tggtgttcca aaagtaacct atatcaaatt ggtaaatgcc gaatcgcctt
     5041 cccgttttgc ccgccgacac atttataatg aattcttata ccgtagtgcc agtaaattgg
     5101 tggctgctga ataagtttta tcccaacata tgtacaaagc catcccattc ggatggtttt
     5161 tttgtggtta aattttacca actacttatt tgcttattta tttaagtatt tttttaaaaa
     5221 ttctttcaaa ctttatttta tgaaataatt atgattaatt ttgttatggt tttaaacact
     5281 tttgatttca atcaatttgt atttttcatg aaatgtaatt gtattaaaag agtactcatt
     5341 taaaaatatt tactttgaaa aaatttattt tcattctctt tttttcgtta tttacaatca
     5401 atacactgga atctcaggtt ttaatatcct tacttttcgg agacaactta aataccggta
     5461 aaatcgagtt tggattagat ggagggatga cactttccga tcaaaccggt ttatcagaca
     5521 gtaaaatgtt gaatagttgg aatttgggtt tttattttga cattaaattg aaagacgatt
     5581 ggatgctaca taccggagtt ttggtaaaat ctaccagtgg ggcagatgaa attccggtgt
     5641 attccttaca aaattctgaa ttggataatt tatatgctac cggttccatt gaacggaata
     5701 tgagttattt taacgtgcca attttgatga aatacaactt taagaatcga ttttatgtag
     5761 aagcagggcc tcaattaggc cttctttaca aagccaccga cgaatttcaa aacaaaattg
     5821 acggcaacga gttaacctat tccaaagatg ttcgggagga atttcatccg ttagacgccg
     5881 gagttgtggg tggggtgggg tatcgcatcc taaaaggata tggtatgaac atcgcaattc
     5941 gttattatta tggattcgtg gatgtgttgg tcaatgattc gggtgacgca gtgaccaatc
     6001 aatcccttta ttttaatgta ggtattccca ttggagtagg aaaagcgtta aaaagagcgg
     6061 aagaaaaaaa gaaagcagaa caaaaacaat aatttaaagc atatatactg tcatgaaacc
     6121 aaattctttt aatccaatca gtttcgttgt acttttaata gttgtacttt ttgctcaaaa
     6181 ttcattcgca caggtttact ccaacaaaga ggtaggtaaa aaaaatcaag agattattga
     6241 tagtttaaaa actgccgaat atccttattc cttacctatt tggggagcaa aagccactga
     6301 aaaaggattc agtcttcctt attctgccgg cataggggtg aattatttag gtcaggaatc
     6361 cgatttaatc attgaaaatt taatggtcgg atttaataat ggtccgctct atgatttgga
     6421 tgaaattgta cggtttaacg gagccacttc taccgccagc gggattaatg tacgacctga
     6481 tatttggttg tttccttttt taaatgtgta tgggattttg gccaaatcta atttatcaac
     6541 cgccatagat gccggtctat gggttccaaa tgctgatggg gtttggagtg aagtaattga
     6601 ttttagtacg aaagctgatt ttcaggcaac tactttcggc attggattta cacctactat
     6661 tggtgtggca gggggttgga ttgcgttaga catgaatgtc acttggtcgg atgttgaggc
     6721 tttagaaaaa cctgcccgag cctttgtgtt tggtccccgt tttggaaaaa cattcaaatt
     6781 gaataaaccg gagcagaatc ttactttctg gactggggca ttccgactaa aattaaacag
     6841 tgaaacgtcg ggttcattac cacttagtga tattctttca acagatggat tacaaacaaa
     6901 ggtagataat gggattatta aagtggaaac cacacaatta caagtagacg actggtggaa
     6961 caatttgact aatgtgcaac aaaacaatcc tgtaaacata gctaaatatg agacggccaa
     7021 cagagctttg gaaacggtag gcggtttttt aaattcgatt gacagtgcct tgaatgatga
     7081 gaatacagca actgttcagt attcattaga caaacgtccg gctgatatgt ggaatttttt
     7141 gttgggaacc caatttcaat ataataaaca ttggatggtt cgtgctgagg taggtttnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta taaaacaaat aattttgcat
     7321 gaatagattt gtatagcctc cggttgcatt tcgaacattg aagttagtgg ataataaggc
     7381 ctttccttca ccggcaataa acatatcttg tgacagtttt atcagtcttg ttttgcctaa
     7441 atttaaacgt tgaatatcat ccgggtgtgt ggcttccata aaaaagtatg gcgaaacatc
     7501 ctcgggctcg ataccaatta tctttttgct ttgtctgctg gtccaaagaa ttttcagttg
     7561 aatgatatca gcaatataaa agaactgatt atttgcctct aataactcat ttaactctaa
     7621 aaaaacagga tcattttcat ttacttcttt aaaaccattc ggcatgaatt ttttgataaa
     7681 ttcataaaaa atactgtaat cctttatata ttctgccatt tattttctcc aaattatttt
     7741 ctcagctgat ttgcctagtt ttttttaaaa tacacattta tagttgttga cttactattt
     7801 aattgattaa taaacaacta cagttggtat gtttaacata aaagagcact aattctattt
     7861 tagaatcctg caaaaataca cttttctaga atgcaattat taaataatcg acctaatcat
     7921 tttttttata aacacagcta atcatgttct aaaggaattg aaaggagaaa atgaactaaa
     7981 actaacagtt ttgaaattta gggtatatca ttatcttctg caatttggaa aatttgtttt
     8041 cgtcaatcgc gaggaagtac ccgaacgata cgatttaaat agataattct atattcgctg
     8101 caacacttcc tttgtattcc ttatttacat tcctatagaa tccctacctt tgcaaaaaga
     8161 ttcatatgac acacaaagca gggttcgtca atatcatcgg aaatccaaat gttgggaaat
     8221 caactttgat gaatgcattc atcggggaga agttatccat tattacttct aaagctcaaa
     8281 ccacgcgtca ccgtattctc ggaattgtga atggggaaga ttttcaagta gttttatcgg
     8341 atacacccgg aattatcaaa ccggcgtatg aaatgcagga atcgatgatg caatttgtaa
     8401 agtctgcctt tgaagatgct gatgtgttgt tgtatatggt agagattgga gagaaagaat
     8461 taaaagatga agcttttttt aataaaatta tccattctaa tattccggtg ttgttgttgt
     8521 taaataaaat tgacaaatct aaccaagaac aattggaaga acaagtggct atgtggcaag
     8581 aaaaagtgcc aaatgctgaa ataatggcca tctctgcctt agaaaatttc aacgtaaaag
     8641 aggtctttaa tcgtattttg gaactgcttc cggaatcgcc accttactac ccaaaagaca
     8701 tgttgacgga caaacccgaa cgtttttttg tgaacgaaac catccgggag aaaatcttac
     8761 tgaactacga taaagaaatt ccgtatgctg tggaggtaga aacggaagaa tttatcgaag
     8821 atgaaaagat tatccgaatc cgttccatca tcatggtcga acgcgatacg caaaaaggaa
     8881 tcattattgg ccataaaggc gaggccttaa aaagagttgg tatgcaatcg cgtcacgatt
     8941 tagaaaaatt cttcggtaaa caaattcata tcgaattgta tgtaaaagtc agcaaagatt
     9001 ggcgtagtaa tgcttttcag ttgagacgat tggggtataa tcagaagtaa gttttaattc
     9061 atatcattca cttattgaat taacaaatta tttttctgtt tgaattcatt caaaatgagt
     9121 atttttaatc aattaattaa aagattgatt cactgtatgc tatgaaaatc ttcgtgattt
     9181 ttttcttctc tttgtttaat gtgactcttt atgcacagaa ggataccatt atatttaaaa
     9241 acaaagatgt tattgttggt gagcttaagc agttgaacaa taatattcta acggttaaaa
     9301 cctcctacag cgacaaagat tttgccatag aatttgataa agtagtttcg ttggtgcttg
     9361 aaaacaaata ttctgtagtc ctttccaatg gattcaggat gtatggttac gttaaaacag
     9421 aaaatccaaa taaggccgtt attactcaag aaaataatgt tgccattgag gtttttctta
     9481 acgaaattgt tgttttacga aaaattgatg atggcttttg gaatcacttt aaaggctctt
     9541 ttgattttgg ttacaatttg acgcaaacaa acaacagtga gcaatttact tttgcaggtc
     9601 tactcaatta ttacagtgaa cgatggattc atagagccac ttacaatcaa ttatttactt
     9661 cacaagatga tgtggaagat attgaacgta ctgattggtc catcgatact aaaagatatt
     9721 tgaaacgaaa atggtttttg aataccaata tatcatttct ttccaatacg agtcagtccc
     9781 taaaaggtcg ttttgtgcct agtgttggag cagggaatta tttagtgaga agtaataaat
     9841 tatatttgtt agtagggaca ggcttgactt ataatatcga aaaatatgag gacgcttcaa
     9901 ccgacaaaac atccaccgaa gctctgttaa ttacacaatt taatatgttt aattttaaag
     9961 atattgattt gtatatctct gcggtaggat acccgagttt gtccgaaagt ggtaggtttc
    10021 gaaccgatgc taacgtccga tttaaatatg atttaccttt cgatttttat tttaaaacgg
    10081 aattgcaggt taattttgac aaccagccgg cggcaggtgc tgtcaaaaca gactatgtgt
    10141 tttctacagg ttttggttgg gaaattaaat aaaaattatt ctaaaattcg ttccattaac
    10201 acttccagtt cttttgcttg gttaattcct tttttccggt ttaatcgagc aatgaaatag
    10261 agtaaaaacc aactgttcac catggcaaac atgaccaata aatcaaaccc gaaaggctga
    10321 tcaagcgacc attttgaaaa ggcaatcatg gaaaaaataa caaatattcc ggctacgaca
    10381 aagtgtaaaa acataaacat tgtccaaagt acgggatcgg gaccatataa tccacggaca
    10441 gctgtattgc catcctccat cttctcgatt tctaaatgta ggtgaggcga atgcttttca
    10501 cgattcagca tcccaatgtg caaccaaatg tggtgacccg aaattttaat cttataatct
    10561 ggctcgacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg cgattcgatt
    10681 gatacgaaat acgatcgttt tggttttgaa tttaatttag tagatacggc cggtatccga
    10741 agaaaagcca aagtaaaaga agatttagaa ttttattctg tgatgcgttc cgttcgtgcc
    10801 attgagcaca gcgatgtgtg tattctaatg gtagatgcca cccgcggatt tgaaggtcag
    10861 gaccaaagca ttttttggtt agctgcaaaa aacagaaaag gaatcatcat tttggtaaac
    10921 aaatgggatt tggtggaaaa agacaccatg tctacgcggg attatgaagc caaaatcaga
    10981 aaagaattag aaccgtttac cgatgttacc atcttatttg tttctgcctt gaccaaacaa
    11041 cgtttgttga aagcattgga agaaaccgtg aaagtatacg aaaacagaaa acaacgcatt
    11101 cctacttcca agttcaacga ttttatgttg aaattgatag aagcctatcc gccaccggcc
    11161 ttaaaaggta aatatgtgaa gattaaatat tgtatgcaat tgccaacacc aacgcctcag
    11221 tttgtgttct ttgccaactt accacaatat gttaaagaag cttataaacg ctttttggaa
    11281 aacaaaattc gggaaaactg ggatttttct ggagttccta ttgatattta cattcgggag
    11341 aaataaactt ttttttaagc agtataattt tttaaatttc ataacgtttt ggtatttgat
    11401 agcccttttt tcaacttaaa atttaattta tgagaaattt aattagtaca gtattcttat
    11461 ttatagccgg attcactaca ttgaatgccc aagaagttgg gattcgtttt ggcgaaatga
    11521 ccggtaataa tgtggctgta gatggtgttt ttagtttggg agagttcagt agaattcatg
    11581 ctgacatttc ttttggtgat ggtttagggg tggatgctct ttgggatttt atctataagc
    11641 ctattgatga cgattttaat tggtatgttg gattcggtcc ttctgttttg tttgatgatc
    11701 ctttttttct gggagttagt ggtgagattg gtatagaata tcattttgat actgtgccca
    11761 ttgctatcgg gttggattat cgaccaacgt tttggattat tgaagagaca gattttgatg
    11821 ccggaggttt tggtttgaat atccgctacg tttttgataa atagttaaaa aaatacttta
    11881 acaaacaagg gattattaat ttgattgata atcccttttt agttaaaata aattttagaa
    11941 taaataataa tttttattat cttcgattaa tggtaaatga tagtattatt agccaattta
    12001 accaaaaata aatttaaatg ttgaaaagtg taattagaat aggaatcatt agcctcttgt
    12061 tttttttaac ggtgtgttgt ggttccacaa tttcagtgac gagtgattat gacaaaggag
    12121 ttgattttaa aaaatacaaa acttttagtt tttatcattt aaaaacaaca ggagaagtaa
    12181 gtcaagtaaa tgcagacagg ttagtgaagg ctattgtgtt ggagatgcaa ggaaagggtt
    12241 tcgttgaatc aagtgacaac ccggatttat tgattaatgc ggttactgtt ataacagaaa
    12301 aagaagcggt ttcagtttct tcagaatatt atggatatgg aggttattat cgtccgtatg
    12361 cttatggata cggttatgga tatggtaccg gttatccggt tgagaatacg gcggctgtca
    12421 atagttataa ttacagagac ggaacaatca tgattgatgt tttagaggct aaatcagaaa
    12481 aattaatttg ggaagggaca ggcaccgctg aaataaacac taagccaagc gacccggcta
    12541 aagtaatagg gtatgctgtt tcaaaaatta tggaaagctt cccacccgaa cccaaaaaaa
    12601 agtaagtaag aatatttaaa aaatttcatt tatgagaaat ttagttgtag gcatatttgc
    12661 cagtatgatg ctcttcagtg gcaatgctca agtaaaaaaa ccagacacta ttgcggtagc
    12721 catttttgat aggatgagtg atgtcattgg cgaaatgtcc tctattagtt ttacccttca
    12781 aattcgtgat gacgagatga accaggattt agggatgatt acccgtttta cagaaagccg
    12841 tgttaatttt aatggcccta atcaaatgca aattcattcc tatggtgata aaggacatcg
    12901 gggtttttgg tttagtggag aatctttagt ttgttattct tttgaagaaa acaactacac
    12961 aattattgat tcgccatcaa atacccttga tatgattaac gaggtgaatg ataaatacgg
    13021 aatcgatttt cctgctgcag atttcttttc acccactttt accgatgatg ttttgtctaa
    13081 ttttgatgag ctaaaatatg ttggaatgag aaccatttcc aacaaagaat gtttttatat
    13141 tatcgccaag agggatgata tgattgtaca gctttggatt gctaatgatg cgttgaattt
    13201 acccgtcaaa atgcttatta tggaaattgg cggaaaagtg acagaacaat atgaagttac
    13261 tttttcaaat tgggacatca atcccttttt accagatgcc atgtttaaat ttcaaacccc
    13321 gcccaaagca aaaaagattt caattttgcc tagaaaataa gaataagtca acaaaattta
    13381 actgatatga aatcaacaaa ttatacatcc tatttatccc taatttctgt attttttacc
    13441 ctactgtttc ttaacgttta tactaccgaa gctcagcgaa tgggacgcgg aggaggggga
    13501 ggaggaggaa gagcctcttc cagtatgtca agacccagtt cttcctatgg tggaggttcc
    13561 gcttcaacca gacaaagttc atcctatgga ggcagttcag catcaacaag acaaagttca
    13621 tcttatggtg gaggttctgc ttcaacgaga cagagttcat cctacggagg ttcctcttca
    13681 gcctcgaata ggcagtctat taatggagga agctataagt ctcctgacag aagtatcaat
    13741 aacccatctg ccggaaattc gtctatgaat agtgccggaa acagagccgg cgaaagacct
    13801 tcaaaaccaa gtactaatga agcgactagt ggaagaaata aagggacttc aaacgataaa
    13861 ctctcctcat ccagtaaacc gggtgtcagt acaaatgacc gaacaaagcc aagcacagga
    13921 tccggcgaca gaaataaacc aagtgccgga acaagtgacc gtaccaaaga caatgttaac
    13981 ggtggtaggg ggaatggaaa taataacaac aataataata ttaatggtgg cagaggaaat
    14041 ggcaacacga atataaatat tcaaaacaat tattacggtc gacctaatac gatgccttat
    14101 tatcgtccgc cttacgctta tggtggatat cattattatt gttatcatcc gtattattat
    14161 catccttata ctccttatta ctatgggcct tactacaatc cttggggtgt atttgtagca
    14221 acaatagccg taaccgctat tgtggttaat tctcaaagtc aacaatatta ttatgacaat
    14281 ggggtttatt acgttcaatc ggatggaggc tatacagtgg tagaagctcc tgccggtgtt
    14341 tccgtaaaag cattgccaaa agaagcacag accgttgtgg taaatgaaac cactaataat
    14401 tattattacg caggaactta ctacgagaaa aagggagatg aatatgttgt ggttcctccg
    14461 acagccggta ccgttgtaga aaatttaccg aaaggtggag aagaggttaa gattggtgag
    14521 gtaacctatg ttcaagtggg taaaacctat tatcaaccca tcaaaaaaga tgggaaagat
    14581 atgtatgaag tagtgaatgt tgaaaaggaa caggaaagca aacagaaata aacctaaact
    14641 atattttatg ttaaacatcg acctacaatt aggtcgatgt tttttttaac taattaaatt
    14701 tcatattact taaaaatcaa gttttataag tatttctttt gaacgtttat aactgtactt
    14761 ttgatattaa ataattgaat aattttaaaa aaagtatttt aaatgaacat cgttcatagt
    14821 aatttttttt gtaaatttat aatgaaatta taaaaccatt tcatttggat tattttattt
    14881 cttaaccctt taacttattt taattatgaa aaaaatgatg gtgattgctt tacttgcaat
    14941 ctttacaatg tctacttatg ctcaacagtc taaagacgaa attgcgattt tacaaagtgc
    15001 ttacggtatg caaaaaagag aacttgtaag caagtacatg aacattagcg aggctcaatc
    15061 agctaccttt tggaaattat atgaagaata tgaagtagct cgtaaagcta ttggcactaa
    15121 aagagccaat aatattttag aatatgccaa aaaatatgat ggcattacgg atgaaaatgt
    15181 tcaaagtctt gtaaaaactt cttttgaagt aaataaagaa ttcaatgcac tttgggagaa
    15241 aacatacaaa caaatggcta aatcattatc tccaaccact gcagccaaat tttatcaatt
    15301 ggaattgtat ttagaaacaa tggttcgaaa tgaactttct gaaagcattc caatgattga
    15361 tgaattgaaa tagttttttt atactttttt taaagccaac ggttgataat cgttggcttt
    15421 tttagtatta taatttatac ttttcaatta cgaaatcaac aaaacccaaa gtacaattaa
    15481 agccgggcca attaaaccg
//
LOCUS       DAOTCW010000052        15104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2293_length_15104_cov_39.233836, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000052 DAOTCW010000000
VERSION     DAOTCW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 15104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15104
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2293_length_15104_cov_39.233836"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="P5335_05935"
     CDS             <1..221
                     /locus_tag="P5335_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRZ74450.1"
                     /translation="PMGPLQLADFIGLDVCLSILNVMYDGFKNPKYAPCPLLVNMVMA
                     GKLGVKSGEGFYDYSESKKAEKVSKQFK"
     gene            291..1508
                     /locus_tag="P5335_05940"
     CDS             291..1508
                     /locus_tag="P5335_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRZ74451.1"
                     /translation="MSKIIPFKAVRPTPDKVALVTCRNYDDYSQAELASWLNFNPYSF
                     LHVIHPAYIHSQKITLDKRFKGVAHKYQDFKNDGIFIEEDKPVFFLYEIQTKTSAFIG
                     IVAGTSIEDYKNNVIKKHEDTLQYRVELFKDYLHQTGFNTEPVLITYPDNPSINQWIE
                     EKKKSVPIYNYSTTNKEKHQLWKIDSDEEIVWLQKQFNIIPELYIADGHHRSASAELL
                     YDQDHHLGNENLNYFMSFLIAESNVKIYEFNRIIRDLNGYSTADFLSKLEEHFILKNK
                     EQELWKPQSKFEFGMYLDGKFYALFYKQETNNPTILENLDAQILYNKVLQPLLGIDDL
                     RNDERIEYIPGKQSILTLKELVDEGEFEVGFMLYPSDISEVKALADNNLIMPPKSTYI
                     EPKFRSGLMVYEL"
     gene            1596..2261
                     /locus_tag="P5335_05945"
     CDS             1596..2261
                     /locus_tag="P5335_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRZ74452.1"
                     /translation="MSIAENLSEIKSALPPHVTLVAVSKTKPVTDLMEAYNAGQRIFG
                     ENKIQEMTDKWQQMPKDIQWHMIGHVQSNKVKYMIPYVRLIHGVDSLKLLQEINRLAG
                     KWRKKVDCLLQIHIAEEETKFGLNEEELEEMLKQVQHDQEGFENIRIVGLMGMATFTD
                     NQKQIKREFLHLKTIFDKHSHLNTDNCHLTTLSMGMSGDYQLAIECGSNMVRIGSSIF
                     GSR"
     gene            2313..3674
                     /locus_tag="P5335_05950"
     CDS             2313..3674
                     /locus_tag="P5335_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRZ74453.1"
                     /translation="MYAILDIETTGGQYNEEGITEIAIYKFDGHEVVDQFISLVNPEK
                     PIQPFVVKLTGINNAMLRSAPKFYEVAKRVIEMTEDCVIVAHNASFDYRILQTEFRRL
                     GFRFHKQTLCTVELSKKLLPGHESYSLGKLVRALGIPMADRHRASGDAMATVKLFKML
                     LAQDIEKEIVKSMVKSEIKTGMSPKLLDIVESLPSKTGIYYIHNEKGDLIYIGKSKNI
                     KKRVNQHFTGTTNKCKKIQLEVYAVTFEETGSELIALLKESEEIKINKPIYNRSQRKT
                     IFQWALYVVKDENGYIALRLQKADGRKKEITSFVSVQEGKNALFKITEQYNLCQKING
                     LYDTKNACFKYKIKECDGACVGEITPEDYNDRVNEFIESNLFENENMVIIDRGRSLQE
                     RSAVMIENGIYKGYCFYDLNYQITNVEVLKNIIIPMQNNRDTRNIIQGYLRKHKVMKI
                     VKF"
     gene            3708..4547
                     /locus_tag="P5335_05955"
     CDS             3708..4547
                     /locus_tag="P5335_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRZ74454.1"
                     /translation="MNSVKSSWEIFRQKTHIIIYGTNTVAGRLFDLILLGIILLSVLL
                     VMLETVEGVDIKYHEQLVFLEWIITIFFTIEYILRIITINKPLKYILSFYGIIDLIAT
                     LPMYLSYFFAGTSIFTTIRALRLLRLFKILNHPQFSGQSVHLKESIVASRGKILVFMY
                     FVLISCIIIGSVMYVVEGKEAGFTSIPTSIYWCIVTLTTVGYGDISPITPLGQFIASL
                     VMIMGYGIIAVPTGIVTAELAKNTTPKMPLPNKLHCFECGSETHLSDAKYCQNCGHSL
                     DEN"
     gene            4537..5463
                     /gene="miaA"
                     /locus_tag="P5335_05960"
     CDS             4537..5463
                     /gene="miaA"
                     /locus_tag="P5335_05960"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRZ74455.1"
                     /translation="MKTNYLITIVGPTAIGKTALSITLAKHFGCEIISCDSRQFFKEM
                     KIGTAVPSEEELKAARHHFIQNKSIFENYSVGDFEREAFEKLDALFQKNTFQIMVGGS
                     GLYVDAVLRGFDDFPDIEPTIREEIRSKYEVKGLTYLQEKLQESDPFYFDKISSENSQ
                     TLQNPQRMMRFVEVCLGTGKPYSSFLNQKKNHRNFIPIIIGLKANREIMYERINRRVD
                     LMIHEGLLEEVKKLHPNKNLNALQTVGYRELFDYLDGKTKLDFAIEEIKKNTRRFAKR
                     QLTWFKRTENVKWFDFETDVSEIVNYITKSLK"
     gene            5517..6065
                     /locus_tag="P5335_05965"
     CDS             5517..6065
                     /locus_tag="P5335_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74456.1"
                     /translation="MKKIVFLFALIPFLFAFQCDPEPGPCGEFIEFEKSDLITIENQQ
                     TTYQVGDLIWLSSIVDRFQVNPITEASYDLFSSDQKLAYSLEFSKTSLYNGSIYLNIN
                     ENTTVIEEGELDWNTIVLTKNDTQFKSKIGIKLLEPGTFTLNIYNIGSFNPDRVGCNF
                     TTYSMKTDFNGIESNLFTFEVE"
     gene            6087..6830
                     /locus_tag="P5335_05970"
     CDS             6087..6830
                     /locus_tag="P5335_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HRZ74457.1"
                     /translation="MPISSNFSSIYSKDWEINFIQCYPNGQLKYTELCNILQLTAGYH
                     AELGGLSFSDMQLHQQAWVLSRMRVEVIELPKWRDVVTVKTWIVDMQGSRSVRALELY
                     VGETKMASSLTYWVVLNTEFRKPEALALPFEHFDIFPENLSTKKSFAKINLNLQTQFL
                     KNKTVLLSDLDIVNHANNVKYLEWCLDCIHPNILLKNGINAFDMNFLRELHWHDEVEL
                     VSSIEEIPNFISVMKNDKYCFALLLEYNP"
     gene            complement(6875..7588)
                     /locus_tag="P5335_05975"
     CDS             complement(6875..7588)
                     /locus_tag="P5335_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRZ74458.1"
                     /translation="METENKKILLVEDDPNFGAILKDYLVLNDFDVVLAKNGMEGFEK
                     FKKDTFDLCILDVMMPYKDGYTLAKEIREKNKEVPLIFLTAKSMKEDVLKGYKVGADD
                     YLNKPFDSEVLLMKIKAIMQRKQSESKTDNLKFEFQIGKLHLNSKLRFLTFGNQEPIK
                     LSPKENELLKMLALHENDLMPRELALTKIWRDDNYFTSRSMDVYIAKLRKYLKMDPDV
                     EILNIHGEGFRLVIKNKSI"
     gene            complement(7611..9191)
                     /locus_tag="P5335_05980"
     CDS             complement(7611..9191)
                     /locus_tag="P5335_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRZ74459.1"
                     /translation="MNRVLFRLLVFLMSISLIGIILVQVYWFNSSLENNQEQFKFHVK
                     QVLGNVSANIQEQEILSFVDKINQFKDSTGSDKVDADFLEFGYYQRNSQTNETIIYSN
                     SINSQDFTISPSFFDKKFDSMKVKSFSAKRKTEIYHNGIDKSNLQNNLRPDLKIEKSG
                     KLKVLDKLLYDISFNDIALTYPIQRRVSKEKLFELLKRQLTEYGINTPFEFGIYSNGL
                     ATKVKSDKFKFNKNDTYSIPIFTDNDGRSQYQLLVSFPEKKKFIFSDLIGIAILSLIF
                     TLIIIIAYANALNQLFKQRQISEIKTDFINNMTHEFKTPIATINLALDAIKNPKVIDD
                     KEKVHRYLQMIRDENKRMHAQVENVLRISKLEKRELEINKEAIDINEIISDAIEHVSL
                     IVEDREGVINTHFSATRTSVLLNDVHFTNVFVNILDNAIKYSPDKPKIDIFTENVKDF
                     LVVKIKDCGAGMSKVAQKRIFEKFYREHTGDLHNVKGHGLGLAYVKRIVEEHNGQITV
                     ESEKGKGSTFIIKLPLIN"
     gene            complement(9263..9847)
                     /gene="coaE"
                     /locus_tag="P5335_05985"
     CDS             complement(9263..9847)
                     /gene="coaE"
                     /locus_tag="P5335_05985"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963935.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRZ74460.1"
                     /translation="MTKIIGLTGGIGSGKSTVANLFMSKGVPVYIADTEAKKLMESRN
                     IITKITNYFGNDILVNSKIDRPKLAKLVFNNPEKLSELNNIVHPEVQKHFQNWLKNKK
                     DFPFVIKEAAILFETGGNKQCDKVITVVAPQELRIQRVKERDKVTREDVLERINNQWT
                     DEMKILVSDFVIENIDIESTKSKVDEILKELNNL"
     gene            complement(9840..10850)
                     /locus_tag="P5335_05990"
     CDS             complement(9840..10850)
                     /locus_tag="P5335_05990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRZ74461.1"
                     /translation="MYFSIIIPVYNRPEEIEELLDSLITQDYNNSFEIVIIEDGSSIS
                     CENIISNYSDKLTIAYFFKENSGPGDSRNFGIKRAKGDYFIIFDSDCIIPTNYLTEVE
                     LALNSDYIDCFGGPDKALDSFSNIQKAINFSMTSFLTTGGIRGGSEKIDKFQPRSFNM
                     GISKKAFEASGGFGNIHPGEDPDLSIRLWKLGFNTKLLTKAYVYHKRRIDWSKFYNQV
                     FKFGKARPILNSWYPEFSKPTFFLPTLFIIGLDLAIIVMFFGLFYPILFYGFYFLLLF
                     FVSSICNKSVKIGSLSLVAAIIQFYGYGFGFLISFILITILKRNPKTEFPELFFKSNK
                     ND"
     gene            complement(10931..13555)
                     /locus_tag="P5335_05995"
     CDS             complement(10931..13555)
                     /locus_tag="P5335_05995"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRZ74462.1"
                     /translation="MKKITLFLLMLFSFTAFAQFPESFETTVPPAGWASFRGTNGLGT
                     GFDWSVSTVAVFVQEGVQSAFVRYEAVTGGSAEDWLVSPMHTVTAPNTLLTFYQRQQY
                     TVDYGTTYDIRVSTTSQTDHATFTTVLSQTEADLNLTTMTVKSVDLSAYVGQSIYIAF
                     VMTNNDGDNWAIDNVNMESSVAAPGCATLLTPADGATIPVGLVNFSWEAPTTGDAPTS
                     YDFYYGIAPDAVDTFLGNYTNTSIDVNVNDYSFTFYWKIVPKNLGGSAIGCPVWTFTT
                     QDPPGYCLSAPNGQWPTDVFTPTTCDGLTANVVTANGYAGEYSVVNVTSGETYIFSSG
                     TTDFITLSSDEGTTATAFGITPLTWVADVTGEVRFYSHVDDQCGDEAVNRVRSIVCGV
                     VTADSPDYVNLQFPSSIDITQGGSGTVYGQIYEAGLTDVEPGLSGQAAGIQAWVGISP
                     VGENTNPSTWTNWVDATHNAASVGNNDEYMAEIGTGLTPGTYYYATRFRLNSGDYVYG
                     GINASNDGNFWDGTTYLSGVLTVNEPVFIGCLNDPNGQYPDATFTPVCTGTVESINNL
                     CYAGEYSNVNLTSGVEYTFSSSIATDRITIGNADGSVVLAYGVTPLTWTSNVTDVVRF
                     YLHTDNACGDNTTFRSRNVFCGTPPPAPANDDCANALALTPGIVFEDYALTTTSLGAT
                     SNVADPLPSCGAFGFDTNGKDVWYSVVVPEAGTITIETQGNEGLVDTAIEAYSGTCGA
                     LVSILCNDDTTGLFSTLSLTGLTAGETILIRAWGYNGSQGSFVISAFDGSLSAPSFDN
                     ANFTYYPNPVKDVLNLSYVSNISKVVVVNLLGQQMITANINATQGQLDLSALASGTYL
                     VKITTEDNLEKTIKVIKQ"
     gene            complement(13645..14457)
                     /locus_tag="P5335_06000"
     CDS             complement(13645..14457)
                     /locus_tag="P5335_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRZ74463.1"
                     /translation="MYNLLKGKRGIIFGALDENSIAWKTAERVHEEGGTFVLTNAPIA
                     MRMGTINQLAEKTGSQIIPADATSVADLENLVEKAVEILGGKLDFVLHSIGMSVNVRK
                     GNHYTSQNYEFTHKGWDVSAVSFHKVMQVLYQKDAMNEWGSVVALTYMAAQRVFPDYN
                     DMADNKAYLESIARSFGYFFGKDKKVRVNTISQSPTPTTAGQGVKGFDGFITYADKMS
                     PLGNATALDCANYTIAMFSDLTKRVTLQNLYNDGGFSNMGVSQDVMEYFTKE"
     gene            complement(14506..>15104)
                     /locus_tag="P5335_06005"
     CDS             complement(14506..>15104)
                     /locus_tag="P5335_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRZ74464.1"
                     /translation="QIILIDLAKNIHLNRKNSLPSLTKMLISILSQLGMPNVQFKIEL
                     TFSDTFFSNGKDAIQFLISTNKGSDFGLLKKVASGGEMSRIMLAVKAVLAQYSSLPTL
                     IFDEIDTGVSGEIANKMGDIMKEMSKNMQIFAITHLPQIAAKGNFHYKVFKSDLGDKT
                     KSEIILLNNDERIIEIAQILSGNSLSDSAINHAKALLN"
BASE COUNT         5227 a         2565 c         2344 g         4968 t
ORIGIN      
        1 acccaatggg acctctgcaa ttggctgatt ttattggttt agatgtttgt ctttccattt
       61 taaatgttat gtacgacgga ttcaaaaacc caaaatatgc tccttgccca cttttagtaa
      121 atatggtgat ggcaggaaaa ctaggtgtaa aatctggaga aggtttttac gattattcgg
      181 agagcaagaa ggcggaaaaa gtttcaaagc aattcaaata aagtatacag tgtttagtaa
      241 ttagttggca gttactgaac acttaattct gccaactgaa cactttttta atgagtaaaa
      301 taatcccttt caaagccgtc cgtccaacac ccgacaaagt cgctttggtt acgtgtagaa
      361 attacgatga ttacagtcag gctgaattag cttcgtggct gaattttaat ccgtactcct
      421 ttttacatgt gattcatccg gcctatatac attctcaaaa aatcacgttg gataaacgat
      481 ttaaaggtgt cgctcataaa taccaagatt ttaaaaacga cggaattttt atagaagaag
      541 acaaaccggt tttctttctg tatgaaatac aaacgaaaac gtctgctttt atcggaattg
      601 ttgccggaac atccattgaa gattacaaaa ataatgtgat caagaaacat gaagatacgc
      661 ttcaatatcg tgtggaatta ttcaaagatt atttgcatca aaccggtttt aatacagaac
      721 ccgttttgat tacgtatccg gataatccat cgattaacca atggatcgaa gaaaagaaaa
      781 aaagtgtgcc catttacaat tattcaacaa ccaataaaga aaagcaccaa ctttggaaaa
      841 ttgacagtga tgaagaaatt gtatggttgc aaaaacaatt taatattatt cctgagttat
      901 acattgccga tgggcatcac cgttctgctt cagcagaatt attatatgac caagaccacc
      961 atttaggcaa cgaaaattta aactatttta tgagtttttt aattgctgaa agcaatgtaa
     1021 aaatttatga attcaaccga attattcgtg atttaaatgg ttattcaaca gcagattttt
     1081 tatctaaatt agaagaacat ttcattctca aaaacaaaga acaggaattg tggaaaccac
     1141 aaagcaagtt tgaatttggt atgtatctcg atggaaaatt ttatgcctta ttttacaaac
     1201 aagaaacaaa taatccgacc attttagaaa acttagatgc tcagatttta tataacaaag
     1261 ttttacaacc tttacttgga attgatgatt taagaaatga cgaacgcatt gaatacattc
     1321 ccggaaagca atcgatttta actttaaaag aattggtcga tgagggcgaa tttgaagtcg
     1381 gcttcatgct ttatccttca gatatttctg aagtaaaagc gttggctgac aataatttga
     1441 ttatgcctcc gaaaagtacg tatatcgaac caaagtttag aagtggattg atggtgtatg
     1501 aattataatt tagataaacg tttgggaaca cagatttaag aaattttaaa gattagaaat
     1561 attccttaaa tctgtgttct aataaaaata aaaaaatgtc aatagcagaa aatctttctg
     1621 aaataaaatc cgctctccca ccacacgtta ctttagttgc ggtttccaaa acaaaaccgg
     1681 ttactgattt gatggaagcg tataatgccg gtcaacgtat ttttggagaa aacaaaatcc
     1741 aagaaatgac ggataaatgg caacaaatgc caaaagacat ccaatggcat atgattggtc
     1801 atgtgcaatc gaataaggtg aaatacatga ttccgtatgt gcgtttgatt cacggtgtgg
     1861 atagtttaaa actgctccaa gagattaatc gattagccgg aaaatggaga aaaaaagtcg
     1921 attgtttatt gcaaatacac attgcagaag aagaaaccaa gtttggattg aatgaagaag
     1981 aattggaaga gatgctgaaa caagttcaac atgaccaaga aggatttgaa aatattcgga
     2041 ttgttggact gatgggtatg gcaacattta ccgataatca aaagcaaata aaaagagagt
     2101 ttctgcattt aaaaactatt tttgacaaac atagtcacct gaatactgac aactgccatc
     2161 tgacaacttt atctatggga atgagcggtg attatcaact cgccattgaa tgtggaagta
     2221 acatggttcg gattggaagt agtatctttg gttcgagata atagagaaaa gagaatagaa
     2281 aatagattaa aaactgaaca cagaacacta ctttgtacgc aattttagac atagaaacca
     2341 ccggaggaca atacaacgaa gaaggaatca ccgaaatagc catctataaa tttgatggtc
     2401 atgaagttgt ggatcaattc atcagtttgg tgaatccgga aaaaccgatt caaccttttg
     2461 tggtaaaatt aaccggaatc aataacgcca tgttacgttc ggcaccaaaa ttttatgaag
     2521 tggccaaacg cgtcatcgaa atgaccgaag attgtgttat cgtggctcat aatgcttctt
     2581 ttgattatcg aattttacaa accgaattcc gaagattagg atttcgtttt cacaaacaaa
     2641 cactgtgtac tgttgaatta tcaaaaaaat tattacccgg acatgaatcg tatagtttag
     2701 gaaaattggt tcgtgcgtta ggaattccaa tggccgatcg acatcgggca agtggtgacg
     2761 cgatggcaac cgtgaaatta ttcaaaatgc ttttggctca agacatcgaa aaagagattg
     2821 tcaaaagcat ggtgaaatcc gaaattaaaa cgggaatgtc accaaaattg ttggatatcg
     2881 ttgaaagctt accttcaaaa accggtatct attacatcca caacgaaaaa ggggatttaa
     2941 tctatattgg taaaagcaaa aatattaaaa aacgagttaa tcagcatttt actgggacta
     3001 ccaataaatg caaaaaaata caattggaag tttatgccgt aacttttgaa gaaaccggca
     3061 gcgaactcat agccttatta aaagaaagtg aagaaataaa aataaataaa ccaatttaca
     3121 atcgttcgca acgcaaaact atttttcaat gggctttgta tgttgtgaaa gatgaaaatg
     3181 ggtatatagc tttacgcctt caaaaagcag atggacggaa aaaagaaatc acttctttcg
     3241 tttccgttca agaaggtaaa aatgctttgt ttaaaataac agaacaatac aacttgtgtc
     3301 agaaaatcaa cggattgtat gatacaaaaa atgcctgttt taaatataaa attaaagaat
     3361 gtgacggagc ttgtgtaggt gaaattacac ctgaggatta caacgaccgt gtaaatgaat
     3421 ttattgaatc caatttgttt gaaaatgaaa atatggtgat tattgaccgt ggcagaagtt
     3481 tgcaggaacg atcggcagtc atgattgaaa atggaattta taaagggtat tgtttttatg
     3541 atttgaatta ccaaatcact aatgtagagg tacttaaaaa catcattata ccaatgcaaa
     3601 acaatcgtga tacccgaaat attattcaag gttacttaag aaaacacaaa gtgatgaaaa
     3661 ttgtaaaatt ttagttttat cttagcacta aagttgaaca aagactcatg aattcagtaa
     3721 aatcatcttg ggaaattttc agacaaaaaa ctcatatcat aatttatggg acaaatactg
     3781 ttgccggaag attattcgat ttgattttat taggaatcat acttttgagt gttttattgg
     3841 taatgctaga aactgttgaa ggagttgata ttaaatatca tgaacaattg gtttttttag
     3901 aatggatcat tactatattt tttaccattg aatatatcct tagaattatc accatcaata
     3961 aaccacttaa atatatttta agtttctacg ggataataga tttaatagca acgctaccta
     4021 tgtacctttc ctatttcttt gccggtacaa gtatttttac aaccattaga gcattgcgat
     4081 tactcagact tttcaaaatc ctcaatcacc ctcaattttc aggacaatcg gtacatttaa
     4141 aagaatcgat tgtggccagt agaggcaaaa tactagtatt tatgtatttt gtgctgatta
     4201 gttgcataat tattggttct gttatgtatg tggtagaagg caaagaagca ggttttacaa
     4261 gtattccaac cagtatctat tggtgtatcg ttacgttaac tacggtaggt tatggagata
     4321 tttcaccgat aactccacta ggacagttta ttgcttcgct ggtaatgatt atgggttatg
     4381 gaattattgc agtaccaaca ggaattgtaa ctgctgaatt agcgaaaaac acaactccca
     4441 aaatgccttt accaaacaaa ttacattgtt ttgaatgcgg gtcggaaaca cacttatccg
     4501 atgcaaaata ttgtcagaac tgcggacatt ccttagatga aaactaatta cctgattaca
     4561 attgtaggtc caactgccat tggtaaaaca gctctaagta ttaccttagc caaacatttt
     4621 ggttgcgaaa ttatttcctg cgacagccga cagtttttca aagaaatgaa aattggaacc
     4681 gctgttcctt ccgaagaaga attgaaagca gcacggcatc attttatcca aaacaaatcc
     4741 atttttgaaa attattcggt aggtgatttt gaacgggaag cttttgaaaa attagatgca
     4801 ctttttcaaa aaaatacctt tcaaatcatg gtgggtggtt ctggattata tgtagatgcc
     4861 gttttgagag gttttgatga ttttccggat atcgaaccta caatccgtga agaaatcaga
     4921 agtaaatatg aagtgaaagg tctcacttat ctacaagaaa aactgcaaga atcagatcct
     4981 ttttattttg ataaaattag ttctgaaaat tcgcagactc tgcaaaatcc gcaacgaatg
     5041 atgcgatttg tagaagtttg tttgggtaca ggtaaaccgt attcttcttt tttaaatcag
     5101 aagaaaaacc accgaaattt tattccgatt atcatcggat tgaaagccaa tcgggaaatc
     5161 atgtatgaga gaatcaatcg aagagtcgac ctcatgatac acgaaggatt gttggaagaa
     5221 gtgaaaaaat tacatcccaa caaaaatctg aatgctctgc aaacagtagg ttatcgtgaa
     5281 ttatttgatt atttggatgg aaaaacaaaa ttagattttg ccattgaaga aatcaaaaaa
     5341 aatacacgtc ggtttgctaa gcgtcaactc acttggttta aacgaaccga aaatgtaaag
     5401 tggtttgatt ttgaaaccga tgtttctgaa attgtaaatt atataactaa atcattaaaa
     5461 taaaaaaatt cacacaacct ttttagtact attgcatctt aaattaaacc ttactgatga
     5521 aaaaaatagt ttttcttttt gcattgattc cctttttatt tgcctttcaa tgtgatcctg
     5581 aaccgggacc ttgtggcgaa tttattgaat ttgaaaaatc ggatttgatt acaattgaaa
     5641 accaacaaac cacctatcaa gtaggcgatt taatttggct ttcgtcaatt gtggacagat
     5701 ttcaagttaa cccaattaca gaagcaagct atgatttgtt ttcttcagat cagaaattag
     5761 cctattcttt agaatttagt aaaacctccc tttacaatgg ttccatttat cttaacataa
     5821 atgaaaacac aaccgtaata gaagaaggtg aactagattg gaacaccatt gttttgacaa
     5881 aaaacgatac acaattcaaa agtaaaattg gaattaaatt attagaaccc ggaacattta
     5941 ctttgaatat atacaatatt ggttctttta atcctgatcg cgttggttgt aattttacaa
     6001 cttattcaat gaagaccgat tttaatggaa tagaaagtaa tttgtttact tttgaagttg
     6061 agtaaaatcg aatttaaaac aatcaaatgc ccatctcttc aaattttagc tccatttatt
     6121 ccaaagactg ggaaataaat ttcattcaat gttatccaaa cggacagttg aagtacaccg
     6181 aattgtgcaa tattttacaa cttacagcgg gctatcatgc agaactgggt ggcttaagtt
     6241 ttagtgatat gcaattgcat cagcaagctt gggttttgag tagaatgcgt gttgaagtga
     6301 ttgaactacc caaatggcga gatgtcgtta ctgtaaaaac ttggattgtc gacatgcaag
     6361 gatcacgttc ggtaagagct ttggaattat atgtaggaga aacaaaaatg gctagttcac
     6421 tcacgtattg ggtcgtttta aatacggaat ttcgaaaacc ggaagcttta gctttaccat
     6481 ttgaacattt tgacattttc cctgaaaatc tatcgacaaa aaaatctttt gccaagatta
     6541 acttgaattt acaaactcag tttttaaaaa ataaaaccgt actcctgtcc gatttagaca
     6601 ttgtcaatca cgcaaataat gtcaaatatt tagaatggtg tttggattgt attcacccaa
     6661 acattttact caaaaatggc atcaatgcat ttgatatgaa ttttttaaga gaattacatt
     6721 ggcatgatga agttgaatta gtatcatcta tagaagaaat tccaaatttc atttcagtga
     6781 tgaaaaatga caaatattgt tttgctcttc ttttggaata taatccatag ttcattataa
     6841 aacaaaaaaa agcctctttt cagaggcttt aagattatat agatttattt ttaataacta
     6901 atcggaaacc ttctccatga atattaagaa tttctacatc cggatccatt ttaaggtatt
     6961 ttcttaattt tgcaatataa acatccatac ttcttgaagt aaaatagtta tcgtctctcc
     7021 aaatttttgt taaagccaat tctcttggca tcaaatcatt ttcgtgaaga gccaacattt
     7081 ttagcaattc attttcttta ggtgacaatt taattggttc ttgattgcca aaagttaaaa
     7141 atcttaactt agaattcaag tgtagctttc caatttgaaa ttcaaatttt aaattgtctg
     7201 ttttactttc agactgtttg cgttgcatga tggctttaat tttcatcaat aacacttcag
     7261 aatcaaatgg tttatttaaa taatcatctg ctccaacttt atatcctttt aaaacatctt
     7321 ctttcattga ctttgctgtc aagaatatca atggcacttc tttatttttt tctctaattt
     7381 cttttgccaa agtgtagccg tctttataag gcatcatcac atccaaaata cacaaatcaa
     7441 aagtgtcttt tttgaatttt tcaaatcctt ccattccatt tttggctaaa acgacatcaa
     7501 aatcatttag cacaagataa tcttttaata tggctccaaa atttggatca tcttctacta
     7561 aaagaatttt tttgttttct gtttccatga gttaatttat ttagtggtat ttaatttatt
     7621 aatggtaatt ttatgataaa ggtactacct tttccttttt cgctttcaac agttatttgt
     7681 ccgttgtgtt cttcaacaat tcgtttgaca taagctaaac ctagtccatg cccttttaca
     7741 ttatgtaaat ctccggtatg ctctcgatag aatttttcga aaattcgctt ttgagctact
     7801 ttactcattc ctgcaccaca atcttttatt ttaactacca gaaaatcttt tacgttttct
     7861 gtaaaaatat caattttagg tttatcaggt gaatacttta tggcgttatc taaaatattt
     7921 acaaatacgt tggtaaaatg aacatcattt aaaagaaccg aagttcttgt tgcactaaaa
     7981 tgtgtattaa ttacaccttc tctatcttca acaattaaac tcacatgttc gatagcatca
     8041 ctaattattt cattaatatc tattgcttct ttattaattt ctaattctct tttttctaat
     8101 ttagaaattc gcaaaacatt ttctacttga gcatgcattc gcttattttc atcacgaatc
     8161 atttgtaaat aacgatgaac tttctcctta tcatcaatga cttttggatt tttaatggca
     8221 tctaaggcaa gatttatagt tgcaataggt gttttgaatt catgggtcat attgttgatg
     8281 aaatctgttt ttatctcgga aatttgacgt tgcttaaata attgattcaa tgcattggca
     8341 taagcaatta taattattaa cgtaaaaatc aatgaaagaa ttgcaatccc tatcaaatca
     8401 gaaaaaataa atttcttttt ttccggaaaa cttactaata attgatattg acttctaccg
     8461 tcattatccg taaaaatagg aatactatac gtatcatttt tattgaattt aaacttatcc
     8521 gatttaactt tagttgctaa gccattacta taaattccaa attcaaaagg tgtatttatt
     8581 ccatattccg tgagttgtct tttcaataat tcgaaaagct tttctttgga aactcttcgt
     8641 tgaataggat aagttaatgc aatatcatta aaagagatat catacaataa tttgtctaaa
     8701 accttaagtt ttcctgattt ttcaattttt aaatcaggtc ttaaattatt ttgcaaattt
     8761 gatttatcaa ttccattatg atagatttca gtttttcgtt ttgcagaaaa acttttcact
     8821 ttcatgctgt caaatttttt atcaaagaac gagggcgaaa tagtaaaatc ttgtgaattg
     8881 atactattcg aataaattat agtttcattt gtttgagaat ttcgttgata atagccaaat
     8941 tccaaaaaat ctgcatctac tttatcactt ccggtactat ccttaaactg atttatttta
     9001 tcaacgaaag ataaaatctc ctgctcttgg atatttgccg aaacattccc taacacctgt
     9061 ttcacatgga atttaaactg ttcttgattg ttttctaaag aagaattaaa ccaataaacc
     9121 tgaactaaaa taattccgat aagtgaaata ctcatcaaaa acactaacag tctaaataac
     9181 actctattca tctttacaaa tttaacattt taacatttgt attgttaata gattaaccaa
     9241 acattaacat catgcacttt tattataaat tatttaattc tttaagaatt tcatctactt
     9301 tggatttagt gctctcaata tcaatatttt caataacaaa atcactcact aaaattttca
     9361 tttcatcagt ccattgatta tttattcttt ctaatacatc ttctctagtt actttatctc
     9421 tttcctttac tctttgaatt cggagttctt gcggagcaac aaccgtaata actttatcac
     9481 attgcttatt gccacctgtt tcaaataaaa tggcagcttc tttaataaca aatggaaaat
     9541 cttttttatt ctttaaccaa ttttgaaaat gcttttgcac ttcagggtga acaatattat
     9601 ttaattcaga aagtttttca ggattattaa atactagttt agccaatttt ggtcggtcaa
     9661 tttttgaatt tactagtata tcattaccaa aataatttgt tattttagta atgatatttc
     9721 ttgattccat taatttttta gcttctgtat cggcaatata aacaggaaca ccttttgaca
     9781 taaataaatt agccacagtt gattttccgc ttccgattcc tcccgttaaa ccaatgattt
     9841 tagtcatttt tatttgattt aaaaaacaat tccggaaatt cagttttggg atttctcttt
     9901 aaaatagtta ttaaaataaa cgaaattaaa aaaccaaaac catatccata aaattgaatt
     9961 atggcagcaa ctaaactaag agatcctatt ttaacacttt tgttacaaat tgaagacaca
    10021 aaaaacaaaa gcaaaaaata aaacccataa aataaaattg gataaaaaag accaaaaaac
    10081 ataactatta ttgccaaatc taaacctata ataaataagg ttggtaagaa aaaagtaggc
    10141 ttactaaatt cagggtacca actgtttaaa attggacgtg cttttccaaa tttgaatact
    10201 tgattgtaaa atttagacca atctattctg cgtttgtgat aaacgtaggc ctttgtcaat
    10261 aatttagtat taaatcctaa tttccacaat cgaattgata aatcgggatc ctcccccgga
    10321 tgaatatttc caaaaccacc cgatgcttca aatgcttttt tagaaattcc catgttgaaa
    10381 cttcttggtt gaaacttatc gattttttca gaacctccac gtattcctcc ggtagttaaa
    10441 aacgaagtca tgctgaaatt aattgctttt tgaatattag aaaaactgtc taacgcttta
    10501 tcaggacctc caaaacaatc aatataatca ctattcaaag ctaattctac ttctgttaaa
    10561 taatttgtcg gaataatgca atcggaatcg aaaatgataa aataatcacc tttagcccgt
    10621 tttataccaa agtttctaga atctcccggt cctgaatttt ctttaaaaaa ataagctatg
    10681 gttagtttat ctgaatagtt agaaattata ttctcacaac ttattgaaga accatcttct
    10741 atgattacaa tttcaaatga attattgtag tcttgagtta tcagactatc aagtaattcc
    10801 tctatttctt ccggacgatt ataaaccggg ataataattg aaaaatacat gttgaaattt
    10861 taacaaatat acagcataaa aaacaaaagc cacgcggtag gcgtggcttt tgaaatcaaa
    10921 ttataaaaaa ttattgtttg ataactttga ttgttttttc aagattatct tcagtagtta
    10981 ttttcactaa ataagttcct gaagctaaag ctgataaatc taattgacct tgtgttgcat
    11041 taatattagc agttatcatt tgttgaccta aaagattaac aacaactact tttgaaatat
    11101 ttgaaacata tgataagttc aatacatctt ttacagggtt tggataatac gtaaaattag
    11161 cattatcgaa tgatggagca cttaatgaac catcgaatgc cgaaatcaca aatgaccctt
    11221 gagagccatt ataaccccaa gctctgatta aaattgtttc gccagctgtt aaaccggtta
    11281 aagataatgt tgaaaacaaa ccagttgtat catcattaca caatatagaa actaatgctc
    11341 cgcaagtccc tgaataagct tcaatagcag tatccactaa accttcatta ccttgtgttt
    11401 caattgtgat tgttcctgct tcaggtacaa caacagaata ccaaacatct tttccattag
    11461 tatcaaaacc aaatgcacca caagatggaa gtggatcagc cacatttgaa gtagcaccca
    11521 aactagttgt tgttaatgca tagtcttcaa aaacaatacc aggagttaaa gctaatgcat
    11581 ttgcacaatc atcatttgca ggtgcaggag gtggagttcc acaaaataca tttctagatc
    11641 tgaaagtagt attatctcca caagcattat ccgtatgtaa atagaatctc actacgtcag
    11701 ttacattaga tgtccaagtt aatggtgtaa ctccataagc taaaacaaca gaaccatctg
    11761 cattaccaat tgtaattcta tcagttgcaa tactactact aaaagtatac tcaacaccag
    11821 aagttaaatt cacatttgaa tattctccag cataacaaag attattaatt gattcaactg
    11881 ttccagtaca tacaggtgta aatgtagcat ctgggtattg accgttaggg tcattcaaac
    11941 aaccaataaa aacaggttcg tttacagtta aaacaccact taaataagta gttccatccc
    12001 agaagttacc atcattagag gcattaatac ctccataaac ataatctcct gaattcagtc
    12061 taaatcttgt tgcataatag taagtcccag gtgttaaacc cgttccaatt tctgccatat
    12121 attcatcatt gtttccaaca ctagctgcgt tgtgagtggc atctacccaa tttgtccaag
    12181 tagatgggtt tgtattttct ccaacaggac tgattccaac ccatgcttga atacctgccg
    12241 cttggccaga aagacctggt tcaacatcag ttaagccagc ttcataaatc tgaccataaa
    12301 cagtacctga tcctccttgt gtaatatcaa tagaagaggg aaactgtaag tttacataat
    12361 ccggtgaatc tgccgttaca acgccacaaa caatagaacg aactctattt actgcctcgt
    12421 caccgcattg gtcatcaacg tgagaataga atctaacttc tccggttaca tctgcaaccc
    12481 aagttaacgg tgtaattcca aaagcagtag cagtagtacc ttcatccgaa cttaatgtaa
    12541 tgaaatctgt tgtaccggaa ctgaaaatat aagtttcacc ggaagtaaca ttcacaacag
    12601 aatattcacc agcataacca tttgctgtta ctacatttgc tgttaaacca tcacaagttg
    12661 ttggtgtaaa aacatctgtt ggccattgtc cgttaggagc acttaaacaa tatcccggag
    12721 gatcttgagt tgtaaatgtc caaactgggc atccaatagc actccctcct aaattttttg
    12781 gaacaatttt ccaatagaat gtaaagctgt aatcatttac gtttacatca atagatgtat
    12841 tagtataatt acctaaaaaa gtatctacgg catccggagc aattccataa tagaaatcat
    12901 acgatgttgg ggcatcccct gtagttggag cttcccatga aaagtttact aaaccaacag
    12961 gaatagtagc tccatcagca ggagtcaaca atgttgcaca accgggagca gctactgatg
    13021 attccatatt cacattatca atagcccaat tgtctccgtc attatttgtc atcacaaagg
    13081 caatgtaaat agactgtcct acatatgcac tcaaatcaac agattttaca gtcattgttg
    13141 ttaaatttaa gtctgcttct gtttgagata gaactgttgt aaatgttgca tgatctgttt
    13201 gagaagttgt tgacacacga atatcatacg ttgttccata gtctacagta tattgttgtc
    13261 tttggtaaaa agtcaacaag gtatttggtg ctgttacagt atgcattgga cttactaacc
    13321 aatcttctgc tgaaccacct gtaacagctt cataacgcac gaatgcagat tgaacaccct
    13381 cttgtacaaa aactgctacg gtagaaactg accaatcaaa tccagttcct aaaccattgg
    13441 ttcccctaaa ggatgcccaa ccagctggtg gaacggtagt ttcaaaactt tcaggaaact
    13501 gagcaaaagc agtaaaactg aaaagcatta ataaaaataa tgtaattttt ttcatattta
    13561 taaagtttaa aattcaaagg ttaaatataa aaaaaaatac ccacaaaatt gtattgtggg
    13621 tatgattttt tataaaactc tttgttattc ttttgtaaaa tattccataa cgtcctgact
    13681 tacacccata tttgaaaatc cgccatcatt atataaattt tgtaatgtca ctcttttggt
    13741 taaatccgaa aacatagcta tcgtataatt agcacaatct aaagcagtgg cattaccaag
    13801 tggcgacatt ttgtcggcat aagtaataaa accatcaaaa cctttaactc cctgtcctgc
    13861 agttgttggt gtaggcgatt gagaaattgt attaactcta acttttttat cttttccaaa
    13921 gaaataacca aaacttctgg cgatagattc taaataggct ttattatctg ccatgtcatt
    13981 ataatcagga aaaacacgtt gggcagccat ataagtcaaa gccacaacac ttccccattc
    14041 attcatagca tctttttgat ataaaacctg cattacttta tggaatgaaa cggcagaaac
    14101 atcccaacct ttgtgtgtaa actcataatt ttggctagtg taatgatttc cttttcgaac
    14161 attgactgac ataccgattg aatgaagaac aaaatccaat ttcccaccta aaatctctac
    14221 tgctttttct actaaatttt ccaaatctgc aactgatgtt gcatcagcag gaataatctg
    14281 agaacctgtt ttttcagcta attgattaat tgttcccatt cgcatagcaa ttggagcatt
    14341 ggtcagaaca aaagttccgc cttcttcatg aacacgctct gctgttttcc aggctattga
    14401 attttcatct aatgctccga atataattcc cctttttcct tttagtaaat tatacataac
    14461 tattttaatt ttaatttata tgatgtttca aatatagtca aaaaatcagt ttaacaatgc
    14521 ttttgcatga tttatggctg aatctgataa agaatttcct gaaagaattt gagcaatttc
    14581 gattattctt tcgtcattat ttaacaatat aatttcagat tttgtcttgt ctcctaaatc
    14641 agatttaaaa accttgtaat gaaaattacc tttcgcagca atttgtggca aatgagtaat
    14701 ggcaaaaatc tgcatattct tactcatttc cttcataata tctcccattt tattggcaat
    14761 ttctccagaa acaccagtat caatttcatc aaaaattagg gttggcaaac ttgaatattg
    14821 agctaaaact gctttaacag ctaacataat tcgagacatc tcacctccgg aagctacttt
    14881 tttcaatagt ccaaaatcag accctttatt tgtagaaatc aaaaactgta tagcatcttt
    14941 tccgttcgaa aaaaaggtgt ccgaaaatgt caactcaatt ttaaattgca catttggcat
    15001 gcctaactga ctcaaaatag aaataagcat ttttgtcaaa ctaggtaaac tgttttttct
    15061 gttaaggtga atatttttag ctaaatcaat caaaattatt tgct
//
LOCUS       DAOTCW010000053        15076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2299_length_15076_cov_35.854404, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000053 DAOTCW010000000
VERSION     DAOTCW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 15076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15076
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2299_length_15076_cov_35.854404"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..2996)
                     /gene="secDF"
                     /locus_tag="P5335_06010"
     CDS             complement(27..2996)
                     /gene="secDF"
                     /locus_tag="P5335_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRZ74465.1"
                     /translation="MQSKGLIKFFAILFALVCIYQLSFTFVANKVKDDAKAYAGDDVL
                     KEVKYLDSIGREKVFSLGFTDYTYNEVKDKQINKGLDLEGGINVILQISVKDILNGLA
                     NNSQDPTFNKALADATRNQKGNQTYLDAFFEAFDAESKGKVKLSSPDIFFNKNLDGEI
                     NLKMTDNEVKAVIKRKVNESVLSAFEVLRKRIDKFGVTQPNIQQLGESGRILVELPGA
                     KDVDRIKKLLQSTAQLEFWETFKFEEVGQYLGSVNEALKATEVKVDVKEKASTGIDSL
                     LTDTAKDSLANTKGNNPLFDKMLSYGGGPVLGYFAPKDTAKINGYLKRDSSKALLTPE
                     LRYARFVWGKPTTVDNEKKEKVEAVELYALKTDRTNQAPISGGVVIDARDSFDQFGKP
                     SVTMQMNGKGAKQWEELTGKAFTQKSNIAIVLDNIVYSAPGVSTGPIAGGRSEISGNF
                     TIDETKDLSNVLRAGKLPAKADIVQSDVVGPSLGQEAIDNGMLSFLIGMLSVVLWMIL
                     YYGKSGIYANISLLVNILFIFGILASLGAVLTLPGIAGIVLTIGMAVDANVIIFERAK
                     EELDAGKTVEEAVNYSYTWTGAMSSIFDANITTALTALILLVFGTGPIKGFATTLLIG
                     IGTSLFTAIFITRIFLDWSVRKNHSLTFSTPMSHNWFKNMNVNFLAKKKLAYGISSVL
                     VIISLVSLFFVNGLNQGVDFVGGRTFQVRFEKEVVASEVSSVLTKTFGTNVEAKTFGT
                     GNQLKITTKYKVSEESEGVDKEVNEKLFEGLKSYFPKTSYEDFVTNSEGKEFGILQAS
                     KVGPTVAEDIKTNSYWAVLGSLLVVFLYLLISFRKWQFSLGAVLAVFHDVIIVLGVYS
                     LFWKVLPFNMEIDQAFIAAILTVIGYSLNDTVIVFDRVREYLGKDVKGTFNDVVNKAL
                     NTTLSRTVNTSLTTLIVLFAIFILGGETIRGFVFAILVGILVGTYSSLFIATPIMADT
                     INKQEARKGAFKEEEKK"
     gene            complement(3188..4123)
                     /gene="mdh"
                     /locus_tag="P5335_06015"
     CDS             complement(3188..4123)
                     /gene="mdh"
                     /locus_tag="P5335_06015"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRZ74466.1"
                     /translation="MKVTIVGAGNVGATCADAIAYRRIASEIVLLDIREGFAEGKALD
                     IMQTQTTLGFNTKVSGSTNDYSKTAGSDVVVITSGIPRKPGMTREELIGINAGIVKTV
                     AESLLQHSPNAIVVVVSNPMDTMTYLTLKATGLPKNRIIGMGGALDSSRFKCYLSQAL
                     NKPANDVSGMVIGGHGDTTMIPLTRLAAYNGVPVSEFLSADELTKVAADTMVGGATLT
                     ALLGTSAWYAPGASVAYLVDSILNDQKKMIPCSVFLEGEYGQSDICIGVPCIIGKNGV
                     EKIVDIKLNEDEKAKFAKSADAVRAMNGDLKSVLA"
     gene            complement(4150..6090)
                     /gene="gyrB"
                     /locus_tag="P5335_06020"
     CDS             complement(4150..6090)
                     /gene="gyrB"
                     /locus_tag="P5335_06020"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRZ74467.1"
                     /translation="MSEEIKKNSYSADSIQALEGMEHVRMRPSMYIGDTGVRGLHHLV
                     YEVVDNSIDEALAGHCDTITVFINEDNSVSVEDNGRGIPVDMHKKEGVSALEVVMTKI
                     GAGGKFDKDSYKVSGGLHGVGVSCVNALSDHLRATVFRDGKIYEQEYERGKAMYPVKQ
                     IGETTKRGTMVTFKPDTSIFTQTIEFSYDTLAARMRELSFLNKGITITMCDKRHLDEK
                     GELVTETFHSKEGLKEFVRFLDGNREPIISHVISMDNEKGEIPVEVALVYNTSYSENI
                     FSYVNNINTHEGGTHLQGFRMGLTRTLKKYADSSGLLDKLKFEISGDDFREGLTAIIS
                     VKVQEPQFEGQTKTKLGNREVVSPVSQSVAEMLENYLEENPNDARIIVQKVILAAQAR
                     HAAKKAREMVQRKTVLGGGGLPGKLSDCSEQDPEKCEIFLVEGDSAGGTAKQGRDRAF
                     QAILPLRGKILNVEKAMHHKVFENEEIRNIFTALGVTIGTAEDSKALNLEKLRYHKVV
                     IMCDADVDGSHISTLILTFFFRFMKELIEEGHVYIATPPLYLVKKGNKKEYAWNDDQR
                     DMINLKLGGSAAIQRYKGLGEMNAEQLWETTLNPNFRTLRKVTIDSLAEADRIFSMLM
                     GDEVPPRREFIEKNAVYAKIDA"
     gene            complement(6345..7961)
                     /gene="asnB"
                     /locus_tag="P5335_06025"
     CDS             complement(6345..7961)
                     /gene="asnB"
                     /locus_tag="P5335_06025"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HRZ74468.1"
                     /translation="MCGILAIIGKGKDEEVVKVLSKRMSHRGPDESDIHVTENGHILA
                     HERLSIIDLHTGKQPIQGSSTAWMIHNGEIYNHQKLRDGILKDHTFRTTSDSEVIVHL
                     YEKFGYDFCDMLDGMFAFVVIDGDDYIAGRDPLGVKPLYYGLDERGRMYFSSEMKSIA
                     DQCKTFSTFPPGHYYTPETGFVKYYQPKWEDETLAVEELDLKAIRETLSEGVRKRLMS
                     DVPIGVLLSGGLDSSLTSSIASRYMKEMGKTLHSFSIGLDAEAPDAKAARKVAEFLGT
                     QHHEIHFTIEQGIEILDKLIWHLETYDVTSVRASTPMYFLSKAITEKGIKVVLSGEGA
                     DEIFGGYLYFRNAPSVEDFQKETIERVQKLFTADLLRADKSTMAHGLEARVPFLDKAF
                     LELAIKIKPEEKMPQTYDGVEKYILRKAFDTPEQPYLPSEVLWRQKEQFSDGVGYNWI
                     DTLIEYCSSQVTDEQLERAEETYPYNTPLTKEAYFYRSIFHKHFPQLAAAQTVRKWIP
                     KWQENQDPSGRANAAHVQADTTIAKIDIPV"
     gene            complement(8140..8583)
                     /locus_tag="P5335_06030"
     CDS             complement(8140..8583)
                     /locus_tag="P5335_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74469.1"
                     /translation="MKQQIIQHYNHYLNDRINSLREMIVDLTEDSLNDAKGSAGDKHE
                     TGLAMMHLEQEKLNRKLQEVVDIQSKFLKIDYSIVSNQVILGSLVSANDTYFLISVSL
                     PKIEIEGKNIFAVSPEAPLAQKMLGAVLGDVITVNQIDYLIRSIE"
     gene            complement(8685..10448)
                     /locus_tag="P5335_06035"
     CDS             complement(8685..10448)
                     /locus_tag="P5335_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ74470.1"
                     /translation="MKRSNLYKSITALFLLGSLLGYSQKKDENIGTEVVNVVKPYTAT
                     ISDAFKVKETPSLNDDEITKKENVQYNIFSFPVASTFTPAKGKAAGVDKTKRDKLYNN
                     YATLGFGNYGRVLGELFVTENISSTDYIGGMFRHHSSQGGIKEVALDDNFYNTSLDLT
                     YGSQQRDYNWNLDLGYQNQIYNWYGIDQTYFNQDAAFYAQIDPNHTFHNAYLGGKIAF
                     EDSFFTGATMKFNRFWDDNGSAENRFYVKPSFVVDINEFAIKTNVIVDYVAGSFDNDY
                     FNNEGYKYGFTNFGLNPSVTLNQDDLSLNIGVSLFYSADLENNDSKFFVYPNISASYK
                     IVGDLMILYAGAEGTLQQNSYREFTNENPFLSPTLIVAPTDRPYDIFAGLKGKLAGNV
                     SYNVRGSFMQEKGRALFRNNPFPNATTPDLEITAKGYQFGNSFDVIYDDMRTVSFFGE
                     LKADFSKNISAGIQGTFSSFSTSNELEAWNLPSITLNANLDLTITEKWYAGASLFFVG
                     ERKDQMIAPGVIDPAFYDFSTITLKSYFDVNAHVGYKFNERLTFFLRMNNMFNQSYEK
                     WMNYPAQQFQVVLGANYKFDF"
     assembly_gap    10482..10581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10582..13307)
                     /locus_tag="P5335_06040"
     CDS             complement(<10582..13307)
                     /locus_tag="P5335_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRZ74471.1"
                     /translation="MQKQNWLLLFLAMAINFTAIAQKTEIYTNDLKEYNRAVELYKEK
                     QYQSAQIIFKNVKEQINNYEVQSDCAYYIANCAIRLDQPNADLLMEQFVTDYPTSSKH
                     NQAFIEVAHYYFEVGKFPQALQWFEKVDESNLTAAEREKFNFQKGYANFSGGKKKEAT
                     TYFNKVVNSKEYGSQAKYYLGYMSYEKDDYKVANEYFEQVEGEQKYKEKMSYYQADMN
                     FKLGNFQKAIDLGTVALSKSDAQEKSELNKIIGESYFNLKQYDKAIPYLKEYKGKKGK
                     WSNTDLYLFGYSYYKQGDYENAIAQFNKIINGNDAVAQNAYYHLGESYLKTDKKQQAL
                     NAFKNASEMPFDVKIQEDAFLNYAKLSYEIGNSYQTIPGVLSAFLEKYPSNPNKPEIE
                     TLLISSFITSKNYKGALELIEKNKSFTNKQAYQKVTFYRGLELYTDGDFTEAMKMFKK
                     SIAEPRNAKFTARATFWKGETEYVLDNFSEALITFKQFLGFPEAKTTPEFKNIHYNIG
                     YAHFKLKEYEQAATQFQSYIDASKDDKVRLNDSYLRMGDCYFVSSKYWPALEAYNKAI
                     ELKGVDADYASYQKALSYGFVNKNDKKIEDLNTFLKNYPKSQYRDDALFELGNTYTNQ
                     NKTDLAIKTYDQLINEYKNGSFAAKSILRQGLIYYNDNKPQLAITKLKKVAADYPRTP
                     EAMEAVTTARQIYVDTGNVSDYADWVKTLDFVEVTNADLDNTSYESAEKQYLQNNAKG
                     AIAGFSNYVSKFPNGIHALNANFYLAQLYFADGLENNAIPNYEYVVSKPRSEFTEQSL
                     ARLTEIHLKKKDYAKAIPALKRLETEADFPQNITFAQSNLMKSYYEQKDYTNAVIYAD
                     KVGANPKVDDRIKSDAQIIIARSAIKTNDEGKAKAAYAKLQLIAKGELAAEAL"
     assembly_gap    13612..13711
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13903..14808)
                     /locus_tag="P5335_06045"
     CDS             complement(13903..14808)
                     /locus_tag="P5335_06045"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRZ74472.1"
                     /translation="MKKSQERKYSMYNAVNNFLKDTDTAIINKMPLMSDAMETLQKNI
                     DKIQDISENQMFNRKGIKVSKEVTKDDIVNVIFVLAGQVRSYAVNTNNVILRDEVTYT
                     FSELKAMADGILQVNSGIIIEKANDNIANLADYGVTAASMTDIEDKMAEFAIKLAQPR
                     NAISNKAEETQLLKAAFAETDQLLEETMDTLVIIVKLTDNQFFNIYKNNRKIIGPGYY
                     TLALRAFVTNPDQEPLEKVKAKIAGNNKIFKTAKKGSFNIKSLPEGMHTITFSLPGYK
                     DQTLNFPIIKGKRTDLRITLQPDNE"
BASE COUNT         4296 a         2725 c         2632 g         5223 t
ORIGIN      
        1 tttaatacta atatgttgat aaagaattat ttcttttctt cttctttaaa tgcacctttt
       61 cttgcttctt gtttgttaat cgtatctgcc ataattggag ttgcaataaa caaagaagaa
      121 taagtaccca ctaagatacc tactaagatt gcaaatacga atccacgaat ggtttctcct
      181 cctaaaataa agatagcaaa caatacaatt aatgttgtta aagaggtatt caccgttcta
      241 cttaaagttg tgtttaacgc tttatttacc acatcattaa aagtaccttt tacatcttta
      301 cctaaatatt cacgtacacg gtcaaataca attacagtat catttaatga gtaaccgatt
      361 acagttagaa ttgctgcaat aaaggcttgg tcaatttcca tgttaaaggg caatactttc
      421 caaaataacg agtaaacacc taatacaata ataacgtcat ggaaaacagc taaaacggcc
      481 cctaaagaga actgccattt tctaaaactg attaacaaat ataagaacac tactaaaagt
      541 gaacctaata ctgcccaata cgaatttgtt ttaatatcct ctgcaactgt tggacctact
      601 ttagatgctt gcaatattcc aaattctttt ccttctgaat tcgttacaaa atcttcataa
      661 cttgtttttg gaaaatacga ttttaaaccc tcaaatagtt tttcatttac ttctttatct
      721 acgccttcac tttcttcact aactttatat ttagtagtaa tttttaactg attaccggtt
      781 ccgaaagttt tggcttcaac attagttcca aaagttttag ttaaaacaga actcacttca
      841 cttgccacaa cttctttttc aaaacgaact tgaaatgtac gacctcctac aaagtcaacc
      901 ccttgattca atccatttac gaaaaataaa gaaacaagag atataataac taaaacactt
      961 gaaataccat aagcaagttt ctttttagcc aagaaattaa cattcatatt tttaaaccaa
     1021 ttgtgtgaca ttggcgttga aaatgtaagt gaatgatttt tacgaacact ccaatctaaa
     1081 aatattcttg taataaaaat agctgtaaat aaagaagttc caatacctat taataaagta
     1141 gttgcaaatc ctttgattgg acctgttcca aaaaccaata aaatcaaggc ggtcaaagcg
     1201 gtcgtaatat tagcatcaaa aattgaagac atagctccag tccatgtata tgaatagttt
     1261 acagcttctt caacagtttt acctgcatct aattcttctt tggctcgttc gaagatgatt
     1321 acgttagcat ccacggccat accaatggtt aacacgatac cggcaatacc aggcaatgtt
     1381 aaaacggctc ctaaacttgc taaaattccg aatataaata atatgtttac taacaatgaa
     1441 atattagcat atatacctga tttaccatag tataaaatca tccataaaac tactgaaagc
     1501 attccaataa gaaatgataa cattccatta tcaatagcct cttgacctaa ggaaggtcca
     1561 accacatcag attgaacgat atccgcttta gccggtaatt taccagctct taaaacgttt
     1621 gataaatctt tggtttcatc aatggtgaaa ttaccagaaa tttcagaacg tccacctgca
     1681 attggtcctg tcgaaactcc aggtgcagaa taaacgatat tatctaatac aatggcaata
     1741 ttactttttt gagtaaatgc ttttcctgtc aattcttccc attgtttagc tcctttaccg
     1801 ttcatttgca tggttacaga aggttttccg aattggtcaa aagaatcacg agcatcaatt
     1861 acaactccac cactaattgg agcttggttt gtacggtcag tttttaaagc atataattca
     1921 accgcttcta ctttttcttt cttctcgtta tcaactgttg ttggttttcc ccaaacaaaa
     1981 cgagcataac gcaattcagg agtcaacaaa gctttgctgc tgtctctttt taaataacca
     2041 ttaattttag ctgtatcttt tggtgcaaaa tagcctaaaa ccggaccacc accatacgac
     2101 aacattttat caaataatgg attattacct tttgtattgg ctaatgaatc tttggcagta
     2161 tctgttaata aagagtcaat tcctgtagaa gctttctctt ttacatcaac tttaacttct
     2221 gtagctttta aggcttcgtt tactgaaccc aaatattgac caacttcttc aaatttgaaa
     2281 gtttcccaaa attctaattg agctgtactt tgtaataatt ttttaatacg atctacgtct
     2341 ttcgcacccg gtaattctac aagaattctt cctgactccc caagttgctg aatatttggt
     2401 tgagtaacac caaatttatc gatacgtttt ctaagtactt caaaagcact taaaaccgat
     2461 tcatttactt ttcttttgat aactgctttt acttcattat cagtcatttt caaattaatt
     2521 tcaccatcta aattcttatt aaagaaaata tccggagaag ataattttac ttttcctttc
     2581 gattcagcat caaaagcttc gaaaaaagca tccaaatagg tttggtttcc tttttgattt
     2641 cttgttgcat cagctaaagc tttgttaaaa gttggatctt gcgagttatt tgctaatcca
     2701 ttcaaaatat ctttaacaga aatttgaaga attacgttaa ttcctccttc aaggtcaagc
     2761 cctttattga tttgtttgtc tttaacttca ttataggtat agtctgtaaa tcctaagcta
     2821 aatacttttt ctctaccaat agagtctaaa tattttactt ctttcaaaac atcatcacct
     2881 gcatatgctt ttgcatcatc tttaacctta ttggctacaa acgtgaatga aagttggtaa
     2941 atacatacca atgcaaataa aattgcgaaa aatttaataa gtcctttact ctgcattatt
     3001 acttgaatta attaattttt gttggttatg gttttgattt acaatacaaa agaaaacaag
     3061 acagttcttt cgattttata aaacgtgcaa atatataatt atgaatagga ttaaccaatt
     3121 aatttgtgca ttaatctcaa aaaaaagact gcaaaaatgc agtctcttta aatctatgtt
     3181 taaaaagtta ggctaaaact gattttaaat caccattcat tgctctaact gcgtctgcac
     3241 ttttagcaaa tttagctttt tcatcctcgt ttagtttaat atcaacgatt ttttcaactc
     3301 catttttacc tataatacaa ggtactccga tacaaatatc gctttgacca tattctcctt
     3361 ctaaaaatac tgaacaaggt atcattttct tttggtcatt caatatacta tcaactaaat
     3421 aggctacaga agctccaggt gcataccatg ctgatgttcc taaaagagct gttaacgttg
     3481 ctcctcctac catagtatca gctgcaactt ttgttaattc atctgcagat aaaaattccg
     3541 aaacaggtac tccattataa gcagccaaac gagttaacgg aatcatagta gtatcgccat
     3601 gtcctccaat aaccattcct gatacatcgt ttgccggttt gttcaaagct tgagataaat
     3661 aacatttgaa acgtgaacta tctaaagctc cacccattcc gatgattctg tttttaggca
     3721 atccggttgc ttttaacgtt aaataagtca tggtatccat tgggttcgaa acaaccacaa
     3781 cgattgcatt aggtgaatgt tgtagtaaac tttctgccac agttttcacg attcctgcat
     3841 taattccgat taattcttca cgagtcatac ccggtttacg tggaattcct gaagtaatca
     3901 ctaccacatc acttcctgcc gtttttgaat aatcatttgt acttccggat acttttgtat
     3961 taaaccctaa agttgtttgg gtttgcataa tatctaatgc ttttccttca gcaaaacctt
     4021 ctctgatatc taacaataca atttcgctgg caattcttct gtaagcaatc gcatcggcac
     4081 atgttgcacc aacatttcct gctcctacaa tggtaacttt cattttttta atttttaaat
     4141 agttatgatt taagcatcaa tttttgcata tactgcattt ttctcaataa attctcttct
     4201 tggcggaacc tcatctccca ttaacataga gaaaattcta tcagcttccg ctaaactatc
     4261 aattgtaact tttcgcaatg ttctaaagtt aggatttaat gtagtttccc atagttgttc
     4321 cgcattcatt tctcccaaac ctttataccg ttgtatagct gcagaacctc ctaatttcaa
     4381 gttaatcatg tcacgttgat catcattcca cgcatattcc tttttattac ctttctttac
     4441 taaatataaa ggaggtgtag caatataaac atgaccttct tcaattaatt ctttcataaa
     4501 acggaagaag aatgttaaaa tcaaagtaga gatgtgacta ccatccacat cggcatcaca
     4561 catgatgact actttgtggt atcttaattt ttctaagttt aatgcttttg aatcttctgc
     4621 agttccaatg gttactccta aagcggtaaa aatattacga atctcctcgt tttcaaaaac
     4681 tttatggtgc atggctttct ccacattcaa aatcttacca cgtaaaggca aaatagcttg
     4741 aaaagcacga tcacgtcctt gtttggctgt tccacctgcc gaatctccct cgactaagaa
     4801 aatctcacat ttttcaggat cttgttctga acaatcagaa agtttacccg gtaatccacc
     4861 gcctcctaaa acggttttac gttgcaccat ctcacgagct ttttttgcag cgtgacgagc
     4921 ttgtgcagcc aaaattactt tttgaacaat aattctggca tcatttggat tttcttccaa
     4981 ataattttct aacatttctg ccaccgattg acttactgga gaaactactt cacggttacc
     5041 taatttggtt ttcgtttgac cttcaaattg aggttcttga acttttacag aaattattgc
     5101 agttaatcct tcacggaaat catctcctga aatttcaaac tttaatttat ctaataatcc
     5161 tgaactatca gcatatttct tcaacgtacg ggttaatccc atacggaaac cttgtaagtg
     5221 agtacctcct tcgtgagtgt tgatgttatt cacataagaa aaaatattct cagaataact
     5281 ggtattgtaa accaacgcca cctcaaccgg aatctctcct ttttcattat ccatgctaat
     5341 cacatgggaa ataattggct ctctatttcc atctaaaaac cgaacgaatt ctttcaaacc
     5401 ttctttagaa tggaaagttt ccgttactaa ttctcctttt tcatccaaat gtcttttgtc
     5461 acacatggta attgtgatgc ctttattcaa aaaggacaat tcacgcatac gtgcggccag
     5521 agtatcatat gaaaattcaa tagtttgcgt aaaaatagag gtgtcaggtt tgaatgtaac
     5581 catggttcca cgtttggtag tttcaccaat ttgcttcacc ggatacattg cttttccacg
     5641 ctcgtattct tgttcatata ttttaccgtc tctgaaaacg gtagctctca aatgatcaga
     5701 aagtgcattc acacaagaaa ccccaacacc gtgaagtcca ccggacactt tataagaatc
     5761 tttatcaaac ttccctcctg ctccaatttt ggtcataaca acctctaaag cagaaacacc
     5821 ttctttcttg tgcatatcaa ccggaatacc ccgaccatta tcttctacag aaactgaatt
     5881 atcttcattt ataaaaactg taatagtatc acaatgtcct gctaacgcct catcgattga
     5941 gttatctact acctcataaa ccaagtggtg taaacctcta actcctgtat caccaatgta
     6001 catagaagga cgcattctca catgctccat tccttctagg gcttgaatac tatctgctga
     6061 ataactattc tttttgattt cttcgctcat aaaaatttaa tgctaaaatg tattttttga
     6121 ataacacgca aatatacgga ttggcaaggg attttctata aaaacacatt agcaaagtgg
     6181 ataagttatt aacaatatgt tgatttttta ttaaaaaaga caactatgtg ttctagtatt
     6241 ttttttaact aaaatagacc taattcttaa ccagttgcca taaaaaaagc gtccccacta
     6301 ataaggacgc ctttcacaaa caaactaaac ttctttaaac tattttaaac aggaatatca
     6361 atttttgcaa tggtggtatc tgcctgaacg tgagccgcat tagctcttcc gcttgggtcc
     6421 tgattttctt gccatttcgg aatccattta cgcaccgttt gtgctgccgc taattgagga
     6481 aaatgtttat gaaaaatact tctataaaaa taagcctctt tcgttagcgg ggtattgtat
     6541 ggataagttt cttctgctct ctctaactgt tcatcggtta cttgactaga acaatattct
     6601 attaatgtat caatccaatt atagcctact ccatctgaaa attgctcttt ttgacgccaa
     6661 agaacttcac ttggtaaata gggttgctcc ggagtgtcaa acgcttttcg aagaatgtat
     6721 ttttcaaccc catcataggt ttgcggcatt ttttcttccg gtttgatttt aattgccaat
     6781 tccaaaaatg ctttatctaa gaaaggaact cgtgcttcga gaccgtgagc catcgttgat
     6841 ttatctgcac gtaataaatc agctgtaaat agcttttgca cacgttctat tgtttctttt
     6901 tggaaatctt ctactgaagg tgcatttcta aaatataaat agcctccaaa aatttcatcg
     6961 gctccttcac cggaaagtac cactttgatt cctttctctg taatggcttt tgataaaaag
     7021 tacattggcg tactagctcg aaccgatgtt acatcatagg tttccagatg ccaaattaat
     7081 ttgtctaaaa tctcaattcc ttgttcaatg gtgaaatgaa tttcatggtg ttgtgttcct
     7141 aaaaattcag ctactttacg agctgcttta gcatcaggag cttccgcatc taatccaatt
     7201 gaaaaggaat gaagtgtttt acccatttct ttcatataac gcgaagctat tgaagaagtt
     7261 aatgaagaat ccaaacctcc tgaaagtaaa acaccgatag gcacatcact cattaatcgt
     7321 ttacggacac cttctgacaa cgtttcacga atggctttta aatccaattc ttccactgct
     7381 aaggtttcat cttcccattt aggttgatag tattttacaa atcctgtttc aggagtgtaa
     7441 taatgtccag gtggaaaagt agaaaatgtt ttacattggt ctgcaattga tttcatttca
     7501 gaagaaaaat acattcttcc tctttcgtcc agaccgtaat agagtggttt tacacccagt
     7561 ggatcacggc cggctatgta atcgtcgcca tcaatcacaa caaaagcaaa cattccatcg
     7621 agcatatcgc aaaaatcata tccaaatttt tcatataaat gcacaatcac ttccgaatca
     7681 gatgtcgttc tgaatgtatg atcttttaaa atcccatctc ttaatttttg gtgattatag
     7741 atttcaccat tgtgtatcat ccaagcagta gaagaacctt gaatgggttg tttcccggtg
     7801 tgtaagtcga taatagacaa acgctcatgg gctaatatat gtccgttttc agttacatga
     7861 atatcacttt catccggacc acggtgtgac attctttttg atagtacttt aactacttct
     7921 tcatcttttc cttttcctat aatggctaat attccacaca taactttttg cttttgtaat
     7981 tttacttaat ttgatgaagc aaaaatcaat attatatgca caattgtaaa cacattatgt
     8041 attattgatt acaatattaa atcaaataat gttattagtt acaaatatga aactaaataa
     8101 tgttgaataa tgaaaagtaa attacaattt ataaaaaacc tattcaatac ttcgaattaa
     8161 ataatcaatt tggtttacag taatgacatc tcccaacacg gctcccaaca ttttttgagc
     8221 taaaggagct tctggagaaa cagcaaaaat gttttttcct tctatttcaa tttttggtag
     8281 actgacggag attaaaaagt aagtatcatt tgctgaaacc aaacttccta aaattacctg
     8341 attggacaca atggagtaat cgattttaag aaatttactt tgtatgtcta caacttcctg
     8401 taatttccgg ttcaattttt cctgctctaa atgcatcatc gctaaacccg tttcgtgttt
     8461 gtcgccggca gaacccttgg catcgttcaa agaatcttcg gttaaatcta caatcatttc
     8521 gcgtaaggaa ttgatgcggt cgttcaggta gtgattgtaa tgttggataa tttgttgttt
     8581 cattctgagt ggctgagtaa ctgagtagct gagtttaaaa agtccgcaag ataaaactct
     8641 ttgcggactc tctgtaaacc tttgcgaact tcgtgattag attcttaaaa atcaaattta
     8701 tagttagctc ctaacacaac ttgaaattgt tgagcaggat agttcatcca tttctcatag
     8761 gattgattga acatgttatt cattcttaag aagaacgtta atcgttcatt gaatttataa
     8821 cctacgtgag cattaacatc aaaataactt tttaatgtaa ttgtggagaa atcataaaac
     8881 gcaggatcga tgactcccgg agctatcatt tggtcttttc tttcgccaac gaagaataaa
     8941 ctggctccgg cataccactt ttcagtaatc gttaaatcaa ggtttgcatt tagggttata
     9001 gaaggcagat tccaagcttc taactcatta ctggttgaaa aactgctgaa ggttccttgt
     9061 attccggcag agatgttttt agagaagtct gcttttagtt cgccaaagaa actaactgtt
     9121 cgcatgtcgt cgtaaatgac gtcaaaggag ttgccgaatt ggtagccttt tgccgtaatt
     9181 tctaagtcag gggttgtggc attagggaaa gggttatttc taaacagtgc tctccccttt
     9241 tcctgcatga aggaaccgcg aacattgtaa cttacattac cggccagctt tccttttaat
     9301 ccggcaaaga tgtcatatgg cctatcggtg ggagcaacga tgagtgtggg agacaggaat
     9361 gggttttcgt ttgtgaactc tctgtaactg ttttgttgta aggttccttc tgcaccggca
     9421 tataaaatca ttaagtcgcc aacgatttta taggaagcag aaatgtttgg ataaacaaaa
     9481 aatttgctgt cattgttttc taaatcggca ctatagaaca gggaaactcc gatgtttaat
     9541 gataaatcat cttggtttaa tgtgacagat ggatttaaac cgaaattagt aaacccgtat
     9601 ttgtatcctt cgttgttaaa gtaatcgttg tcgaaacttc ctgccacgta atcaacaatt
     9661 acatttgttt tgatggcaaa ttcattgata tctaccacaa aagagggttt tacgtaaaac
     9721 cgattttctg ctgaaccgtt atcatcccaa aagcgattga acttcatagt tgctccggtg
     9781 aagaaactat cttcaaaagc tatttttcct cctaaataag cgttatgaaa tgtgtggtta
     9841 ggatctattt gagcatagaa agccgcatct tggttgaaat aagtctgatc aattccatac
     9901 caattataga tttgattttg ataacccaaa tcaagattcc aattataatc gcgttgttga
     9961 ctaccgtagg ttaaatcgag ggaagtgtta taaaaattat cgtctaaggc cacttctttt
    10021 atgccacctt gtgaggaatg atggcgaaac attccgccaa tgtaatccgt actactgata
    10081 ttctcggtga caaacaactc accgagtact cttccgtagt ttccaaaacc gagcgtggca
    10141 taattattat aaagtttatc tctttttgtt ttgtcaactc cggctgcttt cccttttgcg
    10201 ggggtgaatg tggaggctac gggaaaagaa aatatgttgt attgtacatt ttctttcttg
    10261 gtaatctcgt cgtcgttaag ggagggtgtt tcttttactt taaaggcatc ggaaatggtg
    10321 gcggtatagg gttttactac attcactact tccgttccga tgttttcatc cttcttttgc
    10381 gaataaccca acaggctacc caaaagaaac agtgccgtga ttgatttata tagatttgaa
    10441 cgcttcatat cgtttatttt ttgtggtata tattagtttt gnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn nagagcttct gccgctaact caccttttgc aattaattgc
    10621 agtttagcat aagccgcttt tgccttgcct tcgtcatttg ttttgatagc ggaacgagca
    10681 ataatgattt gtgcatcact tttgattcta tcgtctactt ttgggtttgc tcctacttta
    10741 tctgcataga tcactgcgtt cgtatagtct ttttgttcgt aatatgattt catcaagttc
    10801 gattgagcga aggtgatgtt ttgaggaaaa tcagcttcgg tttctaatcg tttcaaagca
    10861 gggattgctt tggcgtaatc ttttttcttt aaatgaattt cagttaaacg ggctaaactt
    10921 tgctcagtaa attcgcttct aggtttagaa actacgtatt catagtttgg aatagcattg
    10981 ttttccagcc catctgcaaa gtacagttgt gctaaataga agtttgcatt taaggcatgg
    11041 atgccgtttg gaaatttaga tacgtaatta ctaaacccgg cgatggctcc tttggcattg
    11101 ttttgcaaat attgtttttc ggctgattca taggaagtat tgtctaggtc tgcgttggta
    11161 acttcaacaa agtcgagggt ttttacccaa tcagcataat cgcttacgtt gccggtgtcc
    11221 acatagattt ggcgagcggt tgtcacggct tccattgctt cgggagttcg gggataatcg
    11281 gctgctactt ttttcaattt tgtgatggct agttgcggtt tgttgtcatt ataataaatt
    11341 aatccctgac gaagaatgga ttttgcggca aaggagccgt ttttgtattc attgatcaac
    11401 tggtcatagg ttttaatggc caaatccgtt ttattttgat tggtgtaggt gtttccgagt
    11461 tcgaaaaggg catcatcacg gtattgtgat ttaggatagt ttttaaggaa ggtgtttaag
    11521 tcttcaattt tcttgtcatt tttgttaacg aagccatagc ttaatgcttt ttgataggag
    11581 gcataatcag catcgacacc ttttagttcg atggctttgt tataggcttc taatgccggc
    11641 caatattttg aggatacgaa ataacaatcg cccattctga ggtaactgtc gttgaggcgg
    11701 actttatcgt ctttcgaggc atcgatgtag ctttgaaatt gtgtagccgc ttgttcgtat
    11761 tcttttaatt tgaaatgggc atatccgata ttgtaatgga tgtttttgaa ttcgggtgtt
    11821 gttttcgctt ccgggaaacc gaggaactgt ttaaaagtga ttaatgcttc ggaaaagttg
    11881 tcgagtacat attctgtttc tcctttccaa aaggttgccc gtgcggtgaa ctttgcattg
    11941 cgaggctctg cgattgattt tttgaacatc ttcatggcct cggtaaagtc gccgtcggta
    12001 tagagttcta atccgcggta aaaggttacc ttttgatagg cttgcttgtt cgtgaagctt
    12061 ttattctttt cgattaactc taacgctcct ttgtagtttt tagaagtgat gaacgaactg
    12121 attaagagtg tttcaatttc gggtttattg gggttgcttg ggtatttttc caaaaaggct
    12181 gacaacaccc cgggaatggt ttggtatgaa ttgcctatct cgtagctcaa tttagcatag
    12241 tttagaaagg catcttcttg aattttgaca tcaaaaggca tttcagatgc gtttttgaag
    12301 gcattgaggg cttgttgctt tttgtctgtt tttaaatagc tttcgccaag gtgataatag
    12361 gcgttttgtg ccacagcatc gtttccgttg atgattttat tgaactgagc aatggcgttt
    12421 tcgtagtcgc cttgtttgta ataggaatag ccgaagaggt aaaggtcggt gttgctccac
    12481 tttccttttt tgcctttgta ttctttgagg taagggatgg ctttgtcgta ttgtttaagg
    12541 ttgaaatagc tttcgccaat gattttattg agttctgatt tttcttgtgc gtcgcttttt
    12601 gataaggcaa ctgttcctaa gtcgatggct ttttgaaagt ttccgagttt gaagttcatg
    12661 tctgcctggt agtaggacat tttttctttg tatttctgtt cgccttccac ttgttcgaaa
    12721 tactcgtttg ccactttgta gtcgtctttt tcataagaca tgtagccgag gtaatatttg
    12781 gcctgtgagc catattcttt agagttcact actttattaa agtaggttgt tgcttctttt
    12841 ttctttccgc cggagaagtt tgcgtagcct ttttggaaat taaacttttc tctttctgct
    12901 gcggtgaggt tgctttcgtc tactttttca aaccattgga gagcttgagg gaacttgcct
    12961 acttcgaagt agtagtgagc cacttcaatg aaggcttggt tgtgtttaga gctggtggga
    13021 taatccgtaa cgaactgctc catgagtaaa tctgcgttgg gctggtctaa tcggatggca
    13081 cagtttgcga tatagtaggc acaatcagat tgtacttcat agttgttgat ttgttctttg
    13141 acgtttttga aaatgatttg ggcagattgg tattgtttct ctttgtataa ctcaactgcc
    13201 cggttgtatt cctttaaatc gttggtatag atttcggttt tttgagcgat ggcggtgaaa
    13261 ttgatggcca ttgcgaggaa aagtagtagc cagttttgct tttgcataag tttcattatt
    13321 gattgattta caaatttagg gtttgcttac tgaagtaacg agtgaatgct tgtgtttatt
    13381 atgaacaagg ttttaaacaa tggttattgg gtaaggtttg acgtgtttgt gttgttgatt
    13441 tagtgaagga gttgttgata ggtcttcgac aaactcagac tgacaaagaa gaaagaggaa
    13501 agaggaaaga ggaaagaagc aagaagcaag aagcaagagg aaagaatata gaaaatagaa
    13561 tatagaaaat agaatataga aaatagaata tagaaaatag aatatagaaa annnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagaatatag aaaatagaat atagaaaata
    13741 gaatatagaa aatagaatat agaaaataga atattgatta tagagtatga ccctcgaagt
    13801 gtttagattt ctttgggggt tattcgctta aaaagcagct ttttagggct gcttttttaa
    13861 ttacgaatta ggaattacga attacgaatt aggaattatt ggttattcgt tatcgggttg
    13921 gagggtgatg cggaggtcgg ttcgttttcc cttgatgatt gggaaattga gggtttggtc
    13981 tttgtagccg ggcaagctga atgttatggt gtgcatgcct tcgggtaggg atttgatgtt
    14041 gaatgagccc ttttttgcgg ttttgaaaat tttgttgttt cccgcaattt ttgcttttac
    14101 tttttctaac ggctcttggt cggggtttgt gacgaaggct cgtagggcta atgtgtaata
    14161 accgggtcct attatcttac ggttgttttt gtagatgttg aagaactggt tgtcggttag
    14221 tttgacgatg attacgaggg tatccatcgt ttcttctaag agctggtcgg tttctgcgaa
    14281 agcggctttg agtaattgtg tttcttccgc tttgtttgaa atggcgttgc ggggttgtgc
    14341 gagtttgatg gcgaattcgg ccatcttgtc ttcaatgtct gtcattgatg cggctgttac
    14401 accatagtca gcgaggtttg caatattgtc gttggctttt tcaatgatga tgcctgaatt
    14461 gacttggagg attccgtctg ccattgcttt gagttcggag aaggtgtagg tcacttcgtc
    14521 tcgtaagata acgttgtttg tgttgacggc ataggaccgc acttgacctg cgaggacaaa
    14581 gatgacattg acgatgtcgt cttttgtgac ttctttgctc accttgattc ctttccggtt
    14641 aaacatttgg ttttctgaaa tgtcttggat tttgtcgatg tttttttgga gggtttccat
    14701 tgcatcggac atcaaaggca ttttgttgat aatggcggtg tcggtgtctt ttaagaagtt
    14761 gtttaccgcg ttgtacattg agtactttct ttcttgtgac tttttcattt tacgtaagtt
    14821 tatgttaacg aattaacgat ttggttgagc aaacctaaac gttattttta aattacaaaa
    14881 tttttatttg cattttttgt taatttgttt cgaaatatat acctgtaatt tttcctacaa
    14941 gtgttaattt taaaggggtt ttaaagattt ttttagttct ttaaattttt tttagttttt
    15001 ttagttttta aaattattct tacagcaatt tttgttttaa agtgcttaaa atgaccggat
    15061 tgattctttg attttt
//
LOCUS       DAOTCW010000054        14976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2320_length_14976_cov_44.796796, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000054 DAOTCW010000000
VERSION     DAOTCW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14976
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2320_length_14976_cov_44.796796"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1063
                     /locus_tag="P5335_06050"
     CDS             5..1063
                     /locus_tag="P5335_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRZ74473.1"
                     /translation="MNKNYYAIVMAGGVGSRFWPVSTTEFPKQFHDMLGSGDTLIQKT
                     FQRLSKLIPIENIFILTNERYNDLVFQQLPMVKQEQVLLEPAMRNTAPCILYASLKIQ
                     KMNPNALMVVAPSDHWIEDENAFNANLQQCFDYCKKENALLTLGIQPTFPNTGFGYIE
                     FDKTDNNPIKKVNQFSEKPDYETAKSFLKSGNFLWNGGIFIWSVQSITEAFLKFQPQM
                     NQLFMNGYSQLNSSEEKEFINQNYAEAENISIDYAILENAQNVFVLPATFDWNDLGTW
                     GSLYDKLDKDEQNNAVVNSKVILENATNNIIRAHDKKLIVIDGLDNFIIVDEGNVLLI
                     YPKDKEQEIKSIMKKATE"
     gene            1083..3449
                     /locus_tag="P5335_06055"
     CDS             1083..3449
                     /locus_tag="P5335_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRZ74474.1"
                     /translation="MIFLIGIDFTLPLKNPVLLFSLILFIILFAPILLNKLRIPHLIG
                     LIIAGAIIGPNGLNLIARDMSIILFGTVGLQYIMFLAGLEIDLAEFKKNSSKSLVFGM
                     LTFSIPMILGTVVGIYVLDFSIATSVLLASMFASHTLIAYPLISKLGVAKNRAVTVAV
                     GGTMITDTLALLVLAVIAGMQTGEINQEFWIRLGFSVILFGLVVMLIFPIIGRWFFKR
                     YDDNISQYIFVLAMVFLGCTLAELAGIEAIIGAFLTGLALNRLIPYTSPLMNRIEFVG
                     NALFIPFFLIGVGMLIDYKAFIKDWDTIQVAGIMTVVAIVGKYLPAYITQKLFGYTRD
                     ERRLIFGLSNAQAAATLAAVLVGFNIIVDYKSVNSYNEVHLKNNKLTTENNQVIVFSE
                     LKKGEYPTLGSIAFVNNIEAKGSFKLNDKTQMSFVNGKLADIKMPNRLLNENVLNGTI
                     LMILITCTIASFVAQKGAYNIALAENDETDSEDDVVIESDEKILIPMKSVDTVDELIN
                     LAVTIKSKKNKTGLFALNVISNNASTAVLESKAQKIVKRAAVVASSTDNSLNMLMRYD
                     LNILNGVSSVVKEHKISDIILGQTESLGASDSLFGPLTDGILNKCKTTTFIYKSVQPL
                     STVKRFIVVIPERAEREIGFPFWLIKIWNLGKNTSSKIVFYGSETTINFIKDIHAKHP
                     VDAEFNLFSDWDDFLILSRHINKDDTLVVVMSRKLNLSYNSVMSNIPGFMNKYFDKNN
                     VLIVYPLQSTLSGSKLDLKSSAALETFTENIERLDDVRKLIGKLFRIK"
     gene            complement(3456..4223)
                     /locus_tag="P5335_06060"
     CDS             complement(3456..4223)
                     /locus_tag="P5335_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRZ74475.1"
                     /translation="MIEIKNIEKSFGDTQVLKGISTIFETGKTNLIIGQSGSGKTVLL
                     KSLLGIHTPDKGTISFDGRIYSELNSNEKRALRTEIGMVFQGSALFDSMTVEENVGFP
                     LTMFSNKTKDEIKERVDFVLKRVNLIDANSKKPSEISGGMQKRVAIARAIVNNPKYLF
                     CDEPNSGLDPKTAIVIDNLIKEITEEYNITTVINTHDMNSVMEIGQKIIFLKEGILAW
                     EGTKKDIFKTDNEAIVDFVYSSNLFKKIRKAQNKEDK"
     gene            complement(4220..4963)
                     /locus_tag="P5335_06065"
     CDS             complement(4220..4963)
                     /locus_tag="P5335_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006264692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRZ74476.1"
                     /translation="MLKILTHIGKYFLMLKEVFNKPLKWSAMKVLIFKEIDDLIIGSL
                     GIVGFISFFVGGVVSIQTALNLTNPLIPRDLIGFATRQSVILEFAPTFISVIMAGKVG
                     SFITSSIGTMRVTEQIDALEVMGVNSLNYLVFPKIIAMLFYPFLIGLSMFLGILGGWM
                     AGVYGGFSNSEDFIRGIQTNFIPFHITYAFIKTLVFAFLLATIPSYHGFYMKGGALEV
                     GKASTVSFVWTSVAIILFNYILTQLLLSK"
     gene            complement(5049..5897)
                     /locus_tag="P5335_06070"
     CDS             complement(5049..5897)
                     /locus_tag="P5335_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRZ74477.1"
                     /translation="MNYYIIIPAHNEEAFIGLTLESIAKQTHLPKKVVIVNDNSTDNT
                     ETIIREFANQHPWASWITISSVASHLPGSKVIQAFQKGLALLDNEYDFIVKADADLIF
                     PTNYFETIQTHFQTDKRIGMVGGFAYIEKKGEWILENLTDKDHIRGAFKAYRKETFKQ
                     IGELKPAMGWDTVDELLCKFYNWSVVTDATLKVKHLKPTGVNYNKNARFKQGEAFYSL
                     GYGFWITAIASAKLAILKRKPLLFFDYMQGFWKAKMAKNPLLVTQEQAEFIKKYRWKT
                     MKEKLF"
     gene            complement(5897..6421)
                     /locus_tag="P5335_06075"
     CDS             complement(5897..6421)
                     /locus_tag="P5335_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRZ74478.1"
                     /translation="MYEKTFPNKRFKITLAFLQKNISTSEKILDLGVENPFSKIMTEN
                     GYHVINTKGEDLDKNQSSLNNENYSVVTAFEIFEHLLNPYTVLQNCKANKILISIPLR
                     LWFSSAYRSKTEKWDRHYHEFEDWQLDWLLEKTGWKIIDKIKFTHPVKKLGFRPLLRY
                     FTNRYYLVYAEKAT"
     gene            complement(6474..7532)
                     /locus_tag="P5335_06080"
     CDS             complement(6474..7532)
                     /locus_tag="P5335_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HRZ74479.1"
                     /translation="MNIKITGSGCYIPTEKISNLDFAKHTFLNDDGTPFPYPNDIVTE
                     KFKEITGIEERRYVTDDLLTSDIAFFAAQKAIENANIDPETLDYIIFAHNFGNVKKGT
                     IQTDLLPSLSSRVKHNLKIKNPNCVAYDILFGCPGWIEGVIQAYAFIKAGMAKKCLVI
                     GAETLSRVVDMHDRDSMIYSDGAGATIIEATEENGGILSHSTATFTYDEAYYLFFGSS
                     FNKTHDQDVRYIKMQGRKIYEFALSQVPKAMAACLDKSGIAISQVKKILIHQANEKMD
                     EAIIQRFYRIYKQTPPEGVMPMSIHELGNSSVATVPTLYDLMARGKLKNQELNKGDVI
                     LFASVGSGMNINAIVYQM"
     gene            complement(7653..>7892)
                     /locus_tag="P5335_06085"
     CDS             complement(7653..>7892)
                     /locus_tag="P5335_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRZ74480.1"
                     /translation="WSQGILGTGGYSKNLTQIHLDVNEKEYLSPELFSIWLNHFYNSV
                     DENFKGEKAERVKTQALSIATVMQIKILNQKKEEI"
     assembly_gap    7893..7992
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7993..8194)
                     /locus_tag="P5335_06090"
     CDS             complement(<7993..8194)
                     /locus_tag="P5335_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRZ74481.1"
                     /translation="MSDIQTQEDLYLLVDEFYKKLLADKTISYIFTDVVKIKLEEHLP
                     ILVTFWSQGILGTGGYSKNLTQI"
     gene            complement(8308..11151)
                     /gene="gcvP"
                     /locus_tag="P5335_06095"
     CDS             complement(8308..11151)
                     /gene="gcvP"
                     /locus_tag="P5335_06095"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRZ74482.1"
                     /translation="MKTDAFALRHLGPREKDLNHMFKTIEVENFDQLIFETIPDDIRL
                     KKDLELDPAMTEYEYLNHIKELGNKNKVYKSYIGLGYNPAIVPAVIQRNIFENPGWYT
                     AYTPYQAEIAQGRLEAILNFQTTVIELTGMEIANASLLDEGTAAAEAMALLFDVRTRD
                     QKKNNCNKFFVSEEILPQTLSVLQTRSTPKGIELVIGNHENFDFSSDFFGAILQYPGK
                     FGQVNDYAKFISKAKEKEIKVTVAADILSLVKLTPPGEFGADVVVGTTQRFGIPMGYG
                     GPHAAFFATKEEYKRSMPGRIIGVTIDANGNRALRMALGTREQHIKREKATSNICTAQ
                     VLLAVMAGMYAVFHGPKGLEYIANKVHSSAVTLANALNKLGVYQTNSSFFDTLLVKAN
                     VQKVKLIAEKHEVNFFYIDSETISISLNETTSINDINQIISIFAEATGKESSSISQLV
                     NESMIPENLVRKSVFLTHDVFNNHHSESQLMRYIKKLERKDLSLNHSMISLGSCTMKL
                     NAAAEMLPLSMANWNNIHPFAPIDQAEGYQIMLKKLEQQLNVVTGFAGTTLQPNSGAQ
                     GEYAGLMTIRAYHLSRGDKHRNVCLIPASAHGTNPASAAMAGMEIIVTKTTENGNIDV
                     EDVRAKAIEHAANLSCLMVTYPSTHGVFESAIREITQIIHDNGGLVYMDGANMNAQVG
                     LTNPATIGADVCHLNLHKTFAIPHGGGGPGVGPICVNEKLVPFLPSNPIISVGGDQAI
                     TAISAAPYGSALVCLISYGYISMLGAEGLKKATEYAILNANYMKARLEEHYPILYSGE
                     CGRAAHEMILDCRAFKQQGVEVTDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESEDLG
                     ELDRFCDALISIRKEIKEATADNHTTVLHNAPHTLAMLTADNWDFPYTREQAAFPLDY
                     ISENKFWPSVRRVDDAYGDRNLVCSCAPIEAYM"
     gene            11432..>11678
                     /locus_tag="P5335_06100"
     CDS             11432..>11678
                     /locus_tag="P5335_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74483.1"
                     /translation="MKKITLFVFFLLGINTLHSQTLVINEIMSTNTSVITDDDGDYQD
                     WIEIYYNGAIAFNLEGYGISDDVTLPYKWVFPEYWIEP"
     assembly_gap    11679..11778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11779..14952
                     /locus_tag="P5335_06105"
     CDS             <11779..14952
                     /locus_tag="P5335_06105"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRZ74484.1"
                     /translation="DDVTLPYKWVFPEYWIEPGDHLVLWCSDKNRTDINNDLHTNFKI
                     GSGGEIITLTKPDGSVADTYPAVVIGNNISYGRQTDGASTLVFFAQPTPWESNTTQGY
                     SEVLVTPSVSAASGFFTSNFPLTVSHPDPSVTIVYTLDGSDPELTNVSGTNYNYKNSY
                     VEIPGQTSGPLLQNNYRSFSYSTPITISDRSSQPNDLAKMSSTYHNVASYVPTFNIFK
                     GTVVRVRAFKAGALPSPIVTKTYFVSPQGSSRFTLPVVSLSLTETKFFEYEDGIFVAG
                     KDFDDWRALNPTEVADNNTVNDVNANANYVRSGSETEQVGNISYFVGGNEVLNQNLGI
                     RINGGNSRNIQSKALRLYARSEYGSDAFNYPFFSDENYTSYKRLVLRNSGQDFYSTLF
                     RDAFTQDLVKTLKLDTKAYQPSIVFVNGEYWGILNLRERYDDKYFKRVYGIDEIDLDY
                     LKDDLTPDTGDNDHYFAMTDYMEANSLDNQANYNYITTQLDPENFRDYNITNIYAQNT
                     DWPGWNTVFWRKRTVAYEPNAPYGQDGRWRAGMNDLDDGFGNYPSGSGHNTLEFATAT
                     GIFDYPNPEWSTLILRKLLENNGFKLDFINRFADMLNTYFLPSRVVSNINLFKARIQP
                     EIQEHIDRWESPETVEVWDDYSIQQMINFANTRPTYQRQHIRNKFGITGDINATLDVD
                     DTTHGYIKINTIEIKSTTPGVSEDPYPWTGIYFQNIPVTLKAIPLPGYIFSHWTGAST
                     STNSEITITSATNFSVTAHFVLSGGPGSEVPIYFWNLDTTIANDTPLTSLNSTFEVPA
                     EGVLQYQSCLTGYPFDATSPNWRKASMERRNSPTDINYIPEANNNIPFATSNMRGIQI
                     KQPFTNNGQQNALIFNVSSLGYRDLKFAFAAKNELAAEGLTFDYSTVSGTPNWISTGL
                     SSSALPLTDAYQLFNIDFVNVPLSDNNQNLKIRVRFTGANLAEDLGNRVTFNNFSVRG
                     VQLNLGVEENTALVFKVFPNPTTELLNIAHTYTKVDYNLYTIDGKLIKSGELETSQIN
                     IGGLQSGIYLLQLNAEGKSDIKKIIKR"
BASE COUNT         4869 a         2380 c         2477 g         5050 t
ORIGIN      
        1 aaaaatgaac aaaaattatt acgcaatagt aatggccggt ggagtaggtt cacgtttttg
       61 gccggtgagt accactgaat tcccaaaaca gtttcacgac atgttgggtt caggcgatac
      121 actgattcag aaaacgtttc aacgattgtc aaaacttatt ccgattgaaa atatttttat
      181 attaaccaat gaacgttaca atgatttggt gtttcaacag ctgccgatgg tgaaacaaga
      241 acaggttttg ttggaacccg ctatgcgaaa taccgctccg tgtattttat atgcttcctt
      301 gaaaattcaa aaaatgaatc cgaatgcttt gatggttgta gctccaagcg atcattggat
      361 tgaagatgaa aatgcattta atgccaattt acaacagtgt tttgactatt gtaaaaaaga
      421 aaatgcgtta ttgactttag gtattcagcc cacttttccc aataccggtt ttgggtacat
      481 cgaatttgat aaaacagata acaatccgat aaaaaaagtc aaccaattta gcgaaaagcc
      541 tgactatgaa acggcaaaat cgttcttgaa aagcggaaat tttctttgga atgggggcat
      601 ttttatttgg tccgtacaat cgataactga agcattttta aaattccaac ctcaaatgaa
      661 tcaattattc atgaatggtt acagtcagct aaatagttca gaagaaaaag aatttataaa
      721 tcaaaattat gcagaagctg aaaatatttc aattgattat gccattttag aaaacgcaca
      781 aaatgttttt gtgttacccg ccaccttcga ctggaatgat ttaggcactt ggggttcatt
      841 atacgataaa ttggataaag atgaacaaaa taatgcagta gttaattcaa aagtaatttt
      901 ggaaaacgca acaaataata ttatcagggc tcatgataaa aaactaattg ttatcgacgg
      961 cttagataat tttattattg tagatgaagg aaatgttttg ctaatttacc caaaagataa
     1021 agagcaagag attaaatcaa ttatgaaaaa agcaacggag taattagaaa taattaaaca
     1081 aaatgatatt tttaataggc attgatttca ctttaccact caagaatccg gttttgctgt
     1141 tctctttgat cttatttatc attctgtttg cacctatttt actgaacaag ttgcgaattc
     1201 cacatctcat aggtttgatt attgccggag cgattattgg tccgaacggt ttaaatttga
     1261 ttgctcgtga tatgagtatt attttatttg gaactgtagg attacaatac atcatgttct
     1321 tagccggttt agaaattgat ttggctgaat ttaagaaaaa cagttctaaa agtttagttt
     1381 ttggaatgct tactttttca attccaatga tattgggaac agttgtgggt atttatgtat
     1441 tggatttttc aattgcaacc tcagtattac ttgccagtat gtttgcttca catactttaa
     1501 ttgcttatcc attaatcagt aaattgggtg ttgccaaaaa cagagctgta actgtagctg
     1561 ttggaggcac catgattact gatactctcg ctcttttagt tcttgcagtt attgcaggca
     1621 tgcaaaccgg tgaaataaat caggaatttt ggattcgttt aggtttttca gtcattcttt
     1681 ttggattggt cgtgatgctt atttttccaa taatagggcg ttggtttttt aaacggtatg
     1741 atgataatat ttcacaatat atttttgtgt tggcgatggt gtttttgggt tgtacactgg
     1801 cggaattagc cggtattgag gctattattg gagctttctt aacgggttta gcattgaatc
     1861 gattaattcc atatacttca cccttaatga atcgtattga gtttgtgggg aatgcacttt
     1921 tcataccgtt tttcttgatt ggtgttggta tgttaattga ttacaaagct tttattaaag
     1981 attgggacac aatccaagta gcaggaatta tgacagttgt ggctattgtt ggaaaatatt
     2041 tacccgcata tattactcaa aagttgtttg gttatacaag agatgagaga cgattaatat
     2101 tcggactaag taacgctcaa gctgctgcga ctctagcggc agttttagtt ggttttaata
     2161 ttattgtaga ttataaaagt gtaaattctt ataatgaagt acatcttaaa aacaataaat
     2221 taacaactga aaacaatcaa gttatagtat tcagtgaatt gaagaaaggg gaatacccaa
     2281 ctttgggaag tattgctttt gttaataata ttgaggcaaa aggctctttt aaattaaatg
     2341 ataaaacaca aatgagtttt gtcaatggaa aattggctga cattaaaatg cctaaccgac
     2401 tactcaatga aaatgtgtta aatggaacca ttttaatgat tttaattact tgtactattg
     2461 cctcatttgt ggctcaaaaa ggagcctata atattgcttt ggctgaaaat gatgaaacgg
     2521 atagtgaaga tgatgttgtg attgaaagcg atgaaaaaat cttaattccg atgaaaagtg
     2581 ttgatacagt tgatgaatta attaacctcg cagtaactat aaaatcgaaa aaaaataaaa
     2641 ccggattatt tgctttgaat gtaattagca ataatgcatc aacagcagtt ttagaaagta
     2701 aagctcaaaa aattgtaaaa agagcagctg tagttgcttc ttcaacagac aattctttaa
     2761 atatgcttat gcgatatgac ttaaacatct taaatggtgt ttcaagtgtt gttaaagagc
     2821 ataaaatatc tgatattatt ctgggtcaaa cagaatctct tggagcatca gattctttat
     2881 ttggaccttt aactgacgga attttaaata aatgtaaaac aactactttt atttataaat
     2941 ctgttcagcc attgtcaaca gtaaaacgat ttatcgttgt cattccggag cgtgcggagc
     3001 gtgaaattgg gtttccgttt tggctcatta aaatttggaa tttaggaaaa aacacgagtt
     3061 ctaaaattgt tttttatggc tctgaaacga caataaattt tataaaagac atccatgcta
     3121 aacatcctgt agatgctgaa tttaatttgt tttctgattg ggatgatttc ttaattttat
     3181 ctcgtcacat caacaaagac gatactttag tagttgtaat gagtagaaaa ttaaatcttt
     3241 cttataactc ggtgatgagt aatattcccg gtttcatgaa taaatatttt gataaaaata
     3301 atgtactgat agtttatccg ttgcaatcta ctttgtcagg aagtaaattg gatttaaaga
     3361 gctcagccgc attagaaacc tttaccgaaa acatagaacg attagatgat gttcgaaaac
     3421 ttatcggtaa attattcaga ataaaataaa ctgtattatt tgtcttcctt gttttgggct
     3481 tttctgattt ttttgaaaag attagaggag taaacaaagt ctactattgc ttcgttatcg
     3541 gttttaaaaa tatctttttt ggttccttcc catgctagga tgccttcttt tagaaagata
     3601 attttttgac caatttccat tacagaattc atatcatgtg tgttgatgac tgttgtgata
     3661 ttatattctt ctgtaatttc cttaattaaa ttatcaataa caatggcagt ttttggatca
     3721 agtccggaat tgggctcatc acagaataaa tattttggat tgtttacgat tgctcttgca
     3781 atagcaaccc gtttttgcat tcctccggaa atttctgagg gtttttttga atttgcatcg
     3841 attaggttga cacgttttaa aacaaaatca acacgttctt tgatttcatc ttttgtttta
     3901 tttgaaaaca tggttaatgg aaaaccaaca ttttcttcta cggtcatgct gtcaaataag
     3961 gcactgcctt gaaataccat gccaatttct gttcgtaaag ctcttttttc atttgaattt
     4021 aattcagaat aaattcttcc atcaaaagaa atggtgcctt tgtcaggagt atgaatacct
     4081 aataaagatt tcaataaaac agttttacct gatccacttt ggccaataat caaattagtc
     4141 tttcctgttt caaaaatggt agaaataccc tttaaaactt gagtatcacc aaatgatttt
     4201 tcaatatttt taatttctat catttgctta ataataattg agttaaaata taattaaaca
     4261 gaataatagc aacggaagtc caaacaaatg ataccgtact tgcttttcca acttctaatg
     4321 caccaccttt catataaaaa ccatggtagg atggaatagt agctaacaga aatgcaaaaa
     4381 ccaaggtttt gataaatgca taggtaatat gaaacggaat gaaattggtt tgtatcccac
     4441 gaataaaatc ttcactattt gaaaaacctc cataaacacc agccatccag ccacctagaa
     4501 ttcctaaaaa catactcagc ccaattaaaa aagggtaaaa taacatagct ataattttgg
     4561 gaaatactaa atagtttaat gaattaaccc ccataacttc taaagcatct atttgctcag
     4621 ttacccgcat agttccgatg ctggaggtga taaatgaacc tactttaccc gccataataa
     4681 cagaaataaa agttggagca aattcaagga taacagattg acgtgtggca aaaccaatta
     4741 aatctcttgg aattaaagga tttgtaagat taagtgctgt ttggatagaa actacgccgc
     4801 caacaaaaaa ggaaataaaa cccacaatgc ccaaagatcc aatgattaaa tcgtcaattt
     4861 ctttaaaaat caagaccttc atcgcactcc atttaagtgg tttgttgaag acttctttta
     4921 acattaagaa atatttccct atgtgggtaa gaatttttag cataaagcaa aaataacaaa
     4981 aagttttggt tactgtttgg cagttttttt tatttgtaaa cgaattaaca ttttcctctt
     5041 tttaaaccct aaaataattt ttctttcatg gttttccatc gatacttttt gataaattca
     5101 gcttgctctt gggttactaa aagtgggttt ttagccattt tagctttcca aaaaccttgc
     5161 atataatcaa aaaaaagtaa tggttttcgt ttaagaatag ctaatttggc tgaggcaata
     5221 gcagtaatcc aaaaaccata tcctaaagaa tagaaagctt caccttgttt aaaacgtgca
     5281 tttttgttat aatttactcc ggttggtttt aaatgtttga ctttcaatgt tgcatctgtt
     5341 actacgctcc aattataaaa tttgcaaagt aattcatcaa ccgtatccca acccattgcg
     5401 ggttttagtt cgccaatttg tttaaaagtt tctttccgat aggctttaaa ggctcctcga
     5461 atatggtctt tgtccgttag attttctaaa atccattctc cttttttttc aatataggca
     5521 aaacctccaa ccattccaat tcgtttatct gtttgaaaat gcgtttgaat tgtttcaaaa
     5581 taattggttg gaaaaattaa atcagcatcg gcctttacaa tgaaatcata ctcattatcg
     5641 agcaatgcta atcctttttg aaatgcttga atcactttac ttcccggtag atgcgaggca
     5701 actgaagata tagtaatcca tgaagcccaa ggatgttgat tagcaaattc ccgaataatt
     5761 gtttccgtat tgtcggtaga attatcattc acaatcacca ctttttttgg caaatgggtt
     5821 tgtttggcaa ttgattcaag cgttaagcca ataaatgctt cttcgttgtg tgccggaatg
     5881 ataatatagt aattcatcat gtagcttttt cagcataaac caaataatac ctattggtga
     5941 aatatcttaa taatgggcga aaacctaatt ttttaactgg atgtgtgaat ttaattttat
     6001 caataatttt ccaaccggtt ttttcaagta accaatccaa ttgccaatct tcaaattcat
     6061 gataatgtct atcccatttt tctgttttac tgcgataggc agatgaaaac catagacgta
     6121 acgggataga aattagaatt ttatttgcct tgcaattttg taaaacagta tatggattta
     6181 ataaatgttc aaaaatttca aaagcagtaa ctacagaata attctcattg ttgagggaac
     6241 tttgattttt atctaaatct tctccttttg tattgataac gtgatatcca ttttcagtca
     6301 ttatttttga gaatggattt tcaactccta aatctaaaat tttttcggat gtggatatat
     6361 ttttttgtaa aaacgcaagc gtgattttga atcgtttatt cggaaatgtt ttttcgtaca
     6421 ttttaaattt taattaaaag atgaaagcaa ttgatagaat tcatttttta aaattacatt
     6481 tgatatacaa ttgcattaat attcattcct gaacctactg aagcaaaaag tataacatct
     6541 cctttgttta attcttgatt ttttaattta cctcgagcca ttaaatcata taaggttggt
     6601 actgtggcaa cgcttgaatt tcctaattca tgaatactca ttggcataac accttcagga
     6661 ggagtttgtt tataaattcg ataaaatcgt tgaataatgg cttcatccat tttctcattt
     6721 gcttgatgaa taaggatttt tttaacttga gaaatagcaa ttccgctttt gtctaaacaa
     6781 gccgccatcg cttttggaac ttgacttaag gcaaattcat atatttttct gccttgcatt
     6841 ttaatataac gaacatcttg gtcatgagtt ttattaaaag aacttccaaa aaataaataa
     6901 taagcttcgt catacgtaaa agtagcagtg gaatgactta gaattcctcc attttcctct
     6961 gtggcttcaa ttattgttgc tccggcacca tcagaataaa tcatggaatc tctgtcgtgc
     7021 atatcaacaa ctctggataa tgtttcagcc ccaatcacta aacatttttt tgccattccg
     7081 gctttaataa atgcataggc ttgaataacg ccttcaatcc atccgggaca accaaatagg
     7141 atgtcatagg ctacacaatt tgggtttttt atcttcaaat tgtgtttaac tcttgaagat
     7201 aaactaggta acaaatcagt ttgaatagtt ccttttttaa cgtttccaaa attatgagca
     7261 aagatgatgt agtcaagtgt ttccggatca atattagcat tctcaattgc tttttgagca
     7321 gcaaaaaaag caatatcaga agtcaacaaa tcatctgtaa catatcttct ctcttcaatt
     7381 cctgttattt ctttaaattt ttccgtaaca atatcgtttg ggtatggaaa tggcgtacca
     7441 tcatcattta aaaacgtatg tttagcaaag tctaaatttg atattttttc agttggaatg
     7501 taacatccgg aacctgttat ttttatattc ataactttat ataatcagtt aaggtacaaa
     7561 attattaatt ataatcgttt tacgtttcga aatcgttcaa taactttttg tttttatgaa
     7621 aaaatcatta attagacttt aaaataagaa tatcatattt cctctttttt ttggttcaaa
     7681 atttttatct gcataaccgt tgcaatgctt aaagcttgtg tttttactcg ttccgctttt
     7741 tcgcctttaa aattttcatc tactgaatta taaaaatgat tcaaccaaat gctaaataat
     7801 tccggtgata aatattcttt ttcattcacg tctaaatgaa tttgtgtcag attttttgaa
     7861 taacctcccg ttcctaaaat cccttgagac cannnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nngaatttgt gtcagatttt ttgaataacc tcccgttcct aaaatccctt
     8041 gagaccaaaa agtaactaat atgggaaggt gctcttctaa tttaatttta acaacatcgg
     8101 taaaaatgta gctgattgtt ttatcagcca gcagtttttt gtaaaattca tcaaccaata
     8161 aatataaatc ttcctgggtt tgtatgtcgg acattttatt gggaaaaagt gaatggttat
     8221 gggtaaatgg taaaagtaaa aaaagtttaa agaagtaacg aaacaacttt aaactttttt
     8281 gaaccttgaa ctattttaat taaaaattta catgtaggct tcaataggag cacaactgca
     8341 aactaaattt ctatcgccat aagcatcgtc aacacggcga acacttggcc agaatttgtt
     8401 ttctgaaata taatctaatg ggaatgccgc ttgttctctg gtatatggaa aatcccagtt
     8461 gtcagcagtc agcatagcta atgtatgcgg agcattgtgt aaaacagtag tatgattgtc
     8521 ggctgttgcc tcttttattt ctttacgaat tgaaatcaac gcgtcacaaa aacggtctaa
     8581 ttctcccaaa tcttctgatt cagttggttc aatcattaat gttccggcta ccggaaaaga
     8641 aacagttgga gcgtggaatc cataatccat caatcgttta gcaatatctg ttacttccac
     8701 accttgttgt ttgaaagcac gacaatctaa aatcatctcg tgagccgctc ttccacactc
     8761 tccggaatac aaaatgggat aatgttcttc taaacgagct ttcatataat ttgcattcaa
     8821 aatagcatat tctgttgctt ttttcaatcc ttcagcacca agcatggaaa tatatccata
     8881 ggaaattaaa caaactaaag cagatccata aggtgctgca gaaattgctg taatcgcctg
     8941 atctccacca acactgataa ttggatttga aggtaaaaaa ggcactaatt tttcattaac
     9001 acatattgga ccaacaccag gaccgccgcc accatgagga atggcaaacg ttttatgcaa
     9061 attcaaatga catacatcag caccaattgt agcaggattt gttaatccaa cttgagcatt
     9121 catattggct ccatccatgt aaaccaatcc tccattatca tgaattattt gtgtgatttc
     9181 acgaatagca ctttcaaaaa caccgtgagt agatgggtat gtcaccatta aacatgataa
     9241 attagcagca tgctcaatag cttttgctct tacatcttct acatcgatat ttccattttc
     9301 agtagttttt gtaacgataa tttccatgcc ggccattgcc gctgaagcag gattagtacc
     9361 gtgtgcagaa gcgggaatta aacaaacatt tcggtgttta tcaccacgag ataaatgata
     9421 agcacgaatg gtcatcaaac cggcatattc tccttgagca ccagaatttg gttgtaaagt
     9481 agttccggca aaaccggtta ctacatttaa ttgttgctct agttttttta gcatgatttg
     9541 ataaccttct gcttgatcaa ttggggcaaa agggtgtatg ttgttccagt tagccatgct
     9601 caaaggaagc atttcggctg cggcatttaa tttcattgtg cacgacccaa gagaaatcat
     9661 ggaatgattt agggacaaat ctttgcgttc aagttttttg atataacgca tcaattgact
     9721 ttcagaatga tggttattaa aaacgtcatg tgttagaaaa acggattttc taaccagatt
     9781 ttccggaatc attgattcat taactaattg gcttatagat gaactttctt ttccagtagc
     9841 ttcagcgaaa atagaaatga tttgattgat atcattaata gaagttgttt catttaatga
     9901 aatagaaatg gtttcactat caatataaaa gaaattaact tcatgttttt ctgcaattaa
     9961 ttttactttt tgaacgtttg cttttactaa aagtgtatca aaaaaagaag aatttgtttg
    10021 ataaacacct aatttattta atgcatttgc aagtgttact gctgaagaat gaactttatt
    10081 ggcaatatat tccaaccctt ttgggccatg aaaaacagca tacattccgg ccataactgc
    10141 caataaaact tgagcggtac aaatgttaga agttgctttt tcacgtttga tatgttgctc
    10201 acgtgttcct aaagccattc tcaaagcacg atttccgtta gcatcgatag ttacaccaat
    10261 gattcttccc ggcattgaac gtttgtattc ttctttagta gcaaaaaagg cagcatgtgg
    10321 tccgccataa cccatcggaa tcccaaaacg ttgcgtggtt ccaacaacaa catcagctcc
    10381 aaattcgccc ggaggagtta attttactaa agataaaata tcagccgcca cggtcacttt
    10441 gatttctttt tctttcgctt tagaaatgaa tttagcataa tcatttactt gaccaaattt
    10501 accgggatat tgtaaaatag caccgaagaa atctgatgaa aagtcaaaat tttcgtgatt
    10561 tcctattact aattcaatcc cttttggagt tgaacgtgtt tgtaaaaccg atagagtttg
    10621 tggtaaaatt tcttcagaaa caaagaattt gttgcaattg tttttcttct gatctcttgt
    10681 tctaacatca aataaaagag ccattgcttc agcagcagca gttccttcgt caagaagaga
    10741 agcattggca atttccattc ctgttaattc aattacagta gtttgaaaat ttaaaatagc
    10801 ttctaaacga ccttgtgcaa tttctgcctg ataaggagta taggcggtat accaacctgg
    10861 attttcaaaa atatttcgtt gaattactgc tggtacaatt gcaggattat agcctaaacc
    10921 tatgtaagat ttataaactt tatttttatt gcctagttcc ttaatatgat tcaaatactc
    10981 atattcggtc atggccgggt ctaattctaa gtcttttttg agtcggatat catccggaat
    11041 cgtttcaaat ataagttggt caaaattttc aacttctatg gttttgaaca tgtgatttaa
    11101 gtctttctca cgtggaccta aatggcgtaa agcaaaggca tctgttttca tttaatttga
    11161 tgaggtaaaa tttgtgttgt tttatgctcg caaaagtaat tattaaggat gtattttttc
    11221 gttaaacaag gactaatatt ttttgaattt ttcaactaaa tgaagaattt atcaacaaaa
    11281 taataaatta ttggtaggct aaaatttata agtttaattt gtttttaaca tttggtcgtc
    11341 ataaaatcac tatttaacga ttatgttatc aaaaaaataa tagattgtaa aatgttgtat
    11401 aattttgtta aatattttaa aacctacgat aatgaaaaaa attactcttt ttgtattttt
    11461 ccttttagga atcaatacat tacatagtca gacattagta ataaatgaaa ttatgtctac
    11521 taatacatct gtcataacag atgatgatgg tgattaccaa gattggattg aaatttatta
    11581 taatggagct attgctttca atttagaagg ttatggtatt tctgatgatg taactttacc
    11641 ttataaatgg gttttcccag aatattggat cgaaccggnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnga tgatgtaact ttaccttata aatgggtttt cccagaatat
    11821 tggatcgaac cgggagatca tttagttcta tggtgttcag ataaaaatag aacagacata
    11881 aataacgatt tacatacaaa ttttaaaata ggttcaggag gtgaaataat tactttaacc
    11941 aaaccagatg gttcagttgc tgatacctac cccgctgttg ttattggcaa taatatatcc
    12001 tacggtagac aaacagatgg tgcttcaacc ttagtttttt ttgcgcagcc aacaccttgg
    12061 gagagcaata caacacaagg atacagtgag gttttagtta ctccttcagt ttctgcagct
    12121 agtggttttt ttacaagtaa ttttcctttg actgtatcac atcccgatcc tagcgtaact
    12181 attgtttata ccttagatgg ttctgaccct gaattaacaa acgtttccgg aacaaattat
    12241 aattataaaa atagttatgt ggaaattccc gggcaaacaa gtggtccact tcttcaaaat
    12301 aattatcgtt catttagtta ttcaactcca attactattt cggatagatc ttctcagcca
    12361 aatgacttgg cgaaaatgtc ttctacttat cacaatgtgg catcttatgt tcccactttt
    12421 aatattttta aaggaactgt agttcgagtt agagcgttta aggctggagc attaccaagt
    12481 ccgatagtaa ctaaaaccta ttttgtttcc ccccaaggat cttcaagatt tacgttgccg
    12541 gttgtctcgc tcagtttaac agaaactaaa tttttcgaat atgaagatgg aatattcgta
    12601 gccggaaaag attttgatga ttggagagct ttaaatccaa cagaagtcgc tgataataac
    12661 accgttaatg atgtcaatgc gaatgctaat tatgttcgtt cagggtctga aacggagcaa
    12721 gtaggtaata ttagctattt tgtaggaggc aatgaagtct taaaccaaaa tttaggaatt
    12781 agaattaatg gtggaaattc cagaaatatt caaagtaaag cactaaggct gtacgctcgt
    12841 tctgaatatg gctcagatgc ttttaattat ccatttttct cagatgaaaa ttataccagt
    12901 tataaaagat tagtacttag aaattctgga caagattttt acagtacttt attcagagat
    12961 gcttttacac aggatttagt taaaacttta aaattggaca caaaagctta tcagccaagt
    13021 attgtttttg tcaatggtga atattggggt attttaaatc ttagggaacg atatgatgat
    13081 aaatatttca aaagagttta cggaattgat gaaattgact tagactattt gaaagatgat
    13141 ttaactccgg atactggtga taatgatcac tattttgcga tgactgacta catggaagcc
    13201 aattctttgg ataatcaggc aaattacaat tatattacaa ctcaattaga tccggaaaac
    13261 ttcagagatt ataacatcac taatatatat gctcaaaaca cggattggcc tggttggaat
    13321 actgtttttt ggagaaaaag aactgtagca tacgaaccaa atgcacccta tggtcaagat
    13381 ggacgatgga gagccggaat gaatgattta gatgatggtt ttggaaatta tccaagtgga
    13441 agcggtcaca atactttaga atttgctaca gcaaccggaa tatttgatta cccaaatccg
    13501 gaatggtcaa cactaatttt acgaaaatta ttagagaata atggttttaa attagatttt
    13561 attaatcgat ttgccgatat gttgaacacc tattttctac cctctagagt agttagtaat
    13621 attaacttgt tcaaagctag aattcaaccg gaaatccaag aacatattga tcgttgggaa
    13681 tctcccgaaa ctgttgaagt ttgggatgat tatagtattc aacaaatgat aaattttgcg
    13741 aataccagac caacatacca aagacaacat attagaaata aatttggtat aactggtgat
    13801 ataaatgcaa cattagatgt agacgatacg acacacggtt atattaaaat taatacaatt
    13861 gaaataaaat ccactactcc tggtgtgtct gaagatcctt atccttggac aggaatatat
    13921 tttcaaaaca ttccggttac tttaaaagca attccattac cgggctatat ttttagtcat
    13981 tggacaggtg cttctacaag cacaaattca gaaatcacta tcacttctgc aaccaatttc
    14041 agtgtgactg ctcatttcgt tctctctggt gggccagggt ctgaagtgcc tatttatttt
    14101 tggaatttag atactacaat tgcaaatgat acaccgctta caagtttgaa ttcaactttt
    14161 gaagttcctg cggaaggtgt tttacaatat caatcctgtt taactggtta tccttttgat
    14221 gcaactagtc caaattggag aaaagcatca atggaaagaa gaaatagtcc aacggatatc
    14281 aattatattc cggaagcaaa taataatatt ccttttgcca cttcaaatat gagagggatt
    14341 caaattaaac aaccttttac aaacaatggt caacaaaatg ctttaatttt taatgtttcc
    14401 tctttaggat atagagattt aaaatttgcg tttgcagcaa aaaatgaatt agcagcggag
    14461 ggacttacat ttgattactc aactgtttca ggaacaccaa actggatttc aacaggcttg
    14521 agttcatctg cattgccatt aacagatgct taccaattat ttaatatcga ttttgtgaat
    14581 gttcctcttt ctgataacaa tcagaactta aaaataagag ttcgatttac cggagctaat
    14641 ttagcagagg acttaggaaa cagagttact tttaacaatt ttagtgttag aggtgtacaa
    14701 ctaaatcttg gtgttgaaga aaacacggca ctggttttca aagtatttcc aaacccaaca
    14761 actgaattgt tgaatattgc tcatacttat acaaaagtgg attataatct ttatacaatt
    14821 gatggaaaac taataaaatc aggagaatta gaaacttctc aaattaatat tggtggcctt
    14881 caaagtggaa tttatttgtt acaacttaat gcggaaggta aatctgatat taagaaaata
    14941 ataaaaagat aactatttta aatcagtttt ttcaaa
//
LOCUS       DAOTCW010000055        14829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2364_length_14829_cov_48.482063, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000055 DAOTCW010000000
VERSION     DAOTCW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14829
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2364_length_14829_cov_48.482063"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2396
                     /gene="sprA"
                     /locus_tag="P5335_06110"
     CDS             <1..2396
                     /gene="sprA"
                     /locus_tag="P5335_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRZ74485.1"
                     /translation="KLDQLIDVTTDEAEKKNIENRAIDYTKRKSINFIGVRKERAPEQ
                     KPKVYNVENFTLSHSYNQVDKHNYEIESYVDQQVNSAVDYSFAFSPKPIEPLKENKMM
                     KKSQYWKMLSDFNFNYLPSNINFSTNVVRQYNRQQFRQVEVEGIALDPLYRRNYLFNY
                     LYGFNYDITKALKINYNANRSNLVRNYIDETGIPDDSNQIYDSYLEVGEANQLNQQFI
                     LNYDIPLYKLPFLSFLKANYSYTGTYSWMRSSLALSSAETEDGTIYNLGNTIQNSSSH
                     RLNTTLNMDLFYKYIGLTKTKKKPSPTPKQAAPKPGEKVTSNKVPQQNEGNVFLDGLI
                     GVVTSVKNIQLNYTQNEGTLLPGYTQSLGFFGSSKPTLGFVFGAQDDVRQEAAKNGWL
                     TIYPEFNQNYTEVKSQQFNGTAKVDLFPDLTIDLTADRNYMESYSEQYDVLDGVYNSR
                     SPYTMGNFSISTVLISTSFSKSDEFGSSAFQDFRDNRLVIANRLAADFYGVNIPRYGD
                     ANNPIPSDPADPKYNFYSSNAGYPIGYGKNNQAVLIPSFLSAYSGKDENSTSLSTFRD
                     FPIPNWTVKYNGLMRYTFFKERFKRFSLQHSYRAAYTINSFRSNFEYDKNPGGQDNEG
                     FGNFYNQTIVQNVNLMEQFNPLIRMDFEMKNSFKMLAEMKKDRALSMSFDNNLLTEVQ
                     GLEYIIGLGYRLKDVVITSSLADNPQGIIKSDINFRTDFSYRNNKTIVRYLDYDNNEL
                     AGGQNIWSVKLTADYSFSKNLTAIFYYDHTFSKAVISTAFPLTNIRSGFTLRYNFGN"
     gene            2466..2846
                     /gene="gcvH"
                     /locus_tag="P5335_06115"
     CDS             2466..2846
                     /gene="gcvH"
                     /locus_tag="P5335_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRZ74486.1"
                     /translation="MNIPTNLKYTKDHEWVSLDGEIATVGITDFAQKELGDIVYVEVE
                     TLDQSLEKDEVFGTVEAVKTVSDLFLPVSGEIIEFNEDLESNPESVNTDPYGKGWMVK
                     VKVSNVDDFDDLLDSQAYKELIGA"
     assembly_gap    3047..3146
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3147..3368
                     /locus_tag="P5335_06120"
     CDS             <3147..3368
                     /locus_tag="P5335_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRZ74487.1"
                     /translation="LLILNLMSKFNFRKAVFISAISAVIYGIIIEVLQGVVTTYREPE
                     ILDILFNSLGCIFAAFLMIKNHERLNFIK"
     gene            3418..4140
                     /locus_tag="P5335_06125"
     CDS             3418..4140
                     /locus_tag="P5335_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRZ74488.1"
                     /translation="MELKKNPNVDPKRNSSLYFQLGLAAIVALSYMAIELKTYEKDLS
                     GLTQKVDNSFLDEEVPITQQLNTPPPPPPPPPPAAPEVIKVVEDKKEIKEMNIQSTET
                     NQQEVVVKAEKVVVVEEEVEVDVPFAIIEDVPLFPGCEGVAKDKRKDCFQEKIQAHIR
                     KNFQYPEAALDAGHQGKVFVSFVIEKDGSVTIANMRAPYPTLEKEAQRIMSKLPKMIP
                     GKQRGKPVRMTMSIPITFKLDQ"
     gene            4241..5005
                     /locus_tag="P5335_06130"
     CDS             4241..5005
                     /locus_tag="P5335_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRZ74489.1"
                     /translation="MRNLDQYPRVNHNSKLYFQLGLIASLIAVYFVIELKSPISEFVV
                     ESKKEVVDISEKPFHETFRIEKEFKEVVEKVKIDKAPSKPILNRFKEVPNTTIEPIQT
                     DVTSPDDIEDNELLLKPIETTAVVLIPTENKIIDRWALEEVPAFKACAKLKGPARETC
                     FNEQMANFLISNLEYPEQAKENGAQESILVEFIINKDGSVSNVKPVFEEKVMNKELEK
                     EALRVVKKLPKFEPGKQAGQPVRVRYTIPITFKIKK"
     gene            5060..5803
                     /gene="deoC"
                     /locus_tag="P5335_06135"
     CDS             5060..5803
                     /gene="deoC"
                     /locus_tag="P5335_06135"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRZ74490.1"
                     /translation="MDIKQFLDSTYLKTPTQAGLSFEENKKIVEGFIAEAIEERFKLI
                     MIRPDMVSLAKKMIAKAESKVLIGTVIGFPEGIYTIEEKLAEATQAIEDGADELDYVC
                     NYENFKNGDSQYVKEEILKCTQLGISHHKTVKWIIEVAALNDQQIIQLSTLIKNVVIA
                     NFKEEQYQDVFVKSSTGFYKTEAGKPNGATVPTIIMMLENSSPLPVKAAGGVRTYEEA
                     IEMISLGVKRIGTSSAKAIAHGNISESNY"
     gene            5821..7932
                     /locus_tag="P5335_06140"
     CDS             5821..7932
                     /locus_tag="P5335_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein RemB"
                     /protein_id="HRZ74491.1"
                     /translation="MKKYSKYFALVLFSVSLWSQVKSTEQFPVFPTCEGKFNIELENC
                     FYAEVQQNIFSKFKVPDKILEKDYKGSFFVLFEVNNQGKFILQYANAPFTELNDECKR
                     VFNEMPQIAPPTYGGNPTYARYSIKISIPLKDANSISNVLSENNENLNTVVVDEKSKQ
                     LNELDSIRYSKFENPQFKSNLNIPFSHSFYAQFDKEMNQLGANNHTASKPLTYAEVNQ
                     YYDLAQANQKLMKNKTGWWGRKLWNEYILEIQGDNYWFTLNPIWDLRVGKSNPSVSDY
                     TYQNTRGIQIQGGIGKEVNFTTSIYESQGRFADYYNNYAESIKPSGGNPAIIPGIGIA
                     KEFKTDSYDFPLADANITYTPSKFFDFQLGYGRNFIGDGYRSLFTSDGASPYPYFKIN
                     TKFWKIKYTNTYMWLKDVTPEATVDGTYASKYMANHYLSWNVSNRLNISFFESVVWTD
                     TNGRGFDFSFVNPIIFYRSVEFNSSSKTGNALLGIASKYKWNNHINLYGQFLIDEFSV
                     SDVKAANQSWRNKFGYQIGVKYFNAFKVDNLLFQLEYNHIRPYVYSHSTPITNYGHNN
                     QSLGHQWGGNAKELLAIARYHQGRWFGDLKLTYGVRGLDFDTPEDDNNYGGNIYKSYN
                     ENRPFDTDVKVGQGNETTIFIADFQAGYLINPTNNLKLFGSLIYRNFDPTQNTLTTFK
                     ETTTWFSVGVRADLFNFYFDY"
     gene            8042..8959
                     /gene="cyoE"
                     /locus_tag="P5335_06145"
     CDS             8042..8959
                     /gene="cyoE"
                     /locus_tag="P5335_06145"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044047593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRZ74492.1"
                     /translation="MNTIQNSFTAKSLYVDFKEITKAGLSVSVVFSSIAGYFLGILDI
                     SSFSWITLFMLAVGGYCMVGASNAFNQIIEKDLDKLMDRTKNRPIAAGRISVNTGFII
                     ASALTLLGLIILYNINPKTAMFGAVSIFLYTSIYTPLKTVTPLSVFVGAIPGAIPFML
                     GWVAATDEFSLEAGTLFLIQFFWQFPHFWAIGWFLFDDYKKAGFFMLPTGKKDKGTAL
                     QTILYTIWLIIASLLPAIFPYIGIVGSRLYLSPISAIIVFLLGIWMLYYAFELYKKRD
                     SKAARKLMLVSVSYITLLQLVYIADKFLR"
     gene            8962..9543
                     /locus_tag="P5335_06150"
     CDS             8962..9543
                     /locus_tag="P5335_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRZ74493.1"
                     /translation="MEMTSQEHLERKGQSYKLLLWFAMISMVMMFAGLTSAYVVSSSR
                     KDWLKDFEMPAAFIISTLVIILSSITFHLAGKSIKANNRSVTTIWLLSTLGLGILFVV
                     FQFLGFNQIIQNGYFFTGSESNVTTSFLYIVVILHLAHLFGGLIALLIIIYNHFKQKY
                     NSTQTLGIELGAMFWHFLDILWILLFLFFYFYE"
     gene            9587..10603
                     /locus_tag="P5335_06155"
     CDS             9587..10603
                     /locus_tag="P5335_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRZ74494.1"
                     /translation="MGATVTTTEEKIWNGGNEPMKASYGKLMMWFFILSDALTFTGFL
                     AAYGFSRFKFIDSWPIADEVFNHFPFLHGQDLPMLYVALMTFVLIFSSVTMVLAVDAG
                     HHMQKSKVAFYMFLTVLGGLIFVGSQAWEWKNFIKGEYGAIETKGGSVIQFVDKEGKR
                     IKIADFALTLPEDRVQHERKNGIWFSKDESSLPTYSVNEVVEGFKANPNILVRTQVTY
                     HDKNDKKGVHPDLNKEKHKTILTREESLARLNNAVYVIEGANLIRNEYGNKQFANFFF
                     FITGFHGFHVFSGVVINFIIFLNVILGTYEKRKNYEMVEKVGLYWHFVDLVWVFVFTF
                     FYLV"
     gene            10624..11064
                     /locus_tag="P5335_06160"
     CDS             10624..11064
                     /locus_tag="P5335_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRZ74495.1"
                     /translation="MAHAHNLAIFRGTLKFKSNEQKIWGVLVFLSMVTAVEVVLGIYK
                     PSFLMTPFMTPFEGGLGATLVNIFLSPFIFLIPLNLIFLFLTIVKAYYITWDFMHMRD
                     ERTSLKRAVVWTFVFLALYIAVLFLTEADYIYEVYRTSTIKWNF"
     gene            11142..11795
                     /locus_tag="P5335_06165"
     CDS             11142..11795
                     /locus_tag="P5335_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74496.1"
                     /translation="MKKNIVLFVLFILPIVAYLFFASGVNNFAKLPTVTANIPDLGNW
                     KTTKGEQLTLDEKITVIAFPGTEILYNKGDAFNLNQKIYNKVREFKDFQLVMIAPIGT
                     ESQTETLKEQLASISDLSKWHYVFAKPEEIQSYYNNLNLIGQLDKNLRTPNVFIVDKK
                     RNLRGRKGKNKKGEAEYKEGYNSISAADLHNEMGDDIKIILAEYRLALKKNSAERIK"
     gene            11801..12487
                     /locus_tag="P5335_06170"
     CDS             11801..12487
                     /locus_tag="P5335_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008989905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRZ74497.1"
                     /translation="MKNKSYIGISFIILVFGIIFIPKIIDRIKNNDVTKIDRLNTSGQ
                     SETSEDLIMKIGPAPKFSLTNQNGETITNDNYKGKVYLVEFFFSTCPTICPIMNQNMV
                     KIQNHFEGKTDFGIVSVTINPENDTSEVLKIHAEHLGVKNPNWNFLTGDKKYIFDLAN
                     KGFNIYAGENKDIDGGFEHSGLFALIDKKGNIRCRKDKFGNPIVYYDGLEETGVQALQ
                     QDIKVLLDEK"
     gene            12489..13052
                     /locus_tag="P5335_06175"
     CDS             12489..13052
                     /locus_tag="P5335_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HRZ74498.1"
                     /translation="MDKQLNSEQPNTIENKFNKLIIIVSIAIPVVVAILFGIKLKDYG
                     INVEPLTFLPPIYAVINGVTAMLLILGVYSIKNGNKKAHQNFMQAAIACSVVFLVMYV
                     AYHMTSDSAIYGGVGFIRYVYFFILISHIILSIAVIPLVLITYVKALAERFDKHKKIA
                     KITFPIWMYVAVTGVIVYLMIAPYYAY"
     gene            13042..13284
                     /locus_tag="P5335_06180"
     CDS             13042..13284
                     /locus_tag="P5335_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74499.1"
                     /translation="MHIKKLKYYLAILLFLAEFSASAQCAMCRAALESEEGGNKAEAI
                     NNGIVYLMAFPYIMVAIVFYVVYRMKFKKKIESEAL"
     gene            complement(13289..14698)
                     /locus_tag="P5335_06185"
     CDS             complement(13289..14698)
                     /locus_tag="P5335_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HRZ74500.1"
                     /translation="MKYLIFPLVLILIVVAFSACSTAKKLEIIKPEPDNAAPLVYETT
                     TSFLKLPVSIKLKDVENQTNKLLNGMIYNDSIMNDDDIIMRVWKQAPIKIESEKEGNI
                     TKIKTTLPLKINATYRYGFDKMGIKLYDTKEFNLNGIVTLLSEVDLTNWQLKTNTKIK
                     SLDWTESPTVTIAGKNIPITYLINPTIRIFKSKIEKGIDDSIKESLNFKPQVLEALDK
                     IATPFEMSEEYASWLRIVPIELYVTDAILKKDAIAMDMGLKCSMETIIGKKPEKKFDK
                     SKIVLKPVAKMPDQITANLIAVSTYEDASIIMTKNFQGQEFGDGKRKVKVTNVNLWHK
                     NGKMIVALDILGSINGTIYLSGFPQYNEETKEIYFDKLDYVLDTKSVLIRSANWLAQG
                     YVLRKIQESCRYSIKPNLEEGKQNVMQYLTNYSPMKGVFVNGTLADFEFQRIELTNKA
                     ILAFIKTSGQVDVKIDGME"
BASE COUNT         5110 a         2115 c         2544 g         4960 t
ORIGIN      
        1 ttaaattaga tcaattgata gatgtcacta cagacgaggc agaaaaaaag aatatcgaaa
       61 atcgtgcaat tgattataca aaacgaaaaa gtataaattt cattggagtg agaaaagaga
      121 gagctcctga gcaaaaacca aaagtttata atgttgagaa cttcacgttg tctcattctt
      181 ataatcaagt tgataaacat aattatgaaa ttgaaagtta tgttgatcag caagtaaatt
      241 ctgcagtaga ttattccttt gcgttttctc caaaaccaat tgaaccattg aaggaaaata
      301 aaatgatgaa aaaatcgcaa tattggaaaa tgttaagtga ttttaatttc aattatttac
      361 cttcgaatat taatttcagt actaatgttg tcagacaata taatcgtcaa caattccgac
      421 aagttgaagt ggagggaatt gctttggatc cattgtacag aagaaattat ttatttaatt
      481 atttgtatgg ctttaattat gatattacga aagctttaaa aattaattat aatgctaacc
      541 gaagtaacct tgttagaaat tatattgatg aaaccggaat tccggatgat tcaaatcaaa
      601 tttatgacag ctatttagaa gttggagaag cgaatcaatt aaatcaacag ttcattctga
      661 attatgatat tcctttatac aaattacctt ttttaagttt cctaaaagca aattattctt
      721 atacaggaac ttatagctgg atgcgatctt ctttagctct ttcatcagct gaaaccgaag
      781 atggaaccat ttacaattta ggaaatacaa ttcaaaattc aagttctcat cgtttgaata
      841 ctacgttaaa catggatttg ttttataaat atattgggtt gactaaaaca aaaaagaaac
      901 cgtcaccaac accaaaacaa gctgctccaa aaccaggtga aaaagtgacc agtaataagg
      961 ttcctcaaca aaatgaagga aatgtttttc ttgacggact gattggtgta gtaacgagtg
     1021 ttaaaaatat tcaattgaat tatactcaaa atgaaggaac attgttaccg ggttatacac
     1081 aatcgttagg gttttttggt tcttcaaaac caacgttagg atttgtgttt ggagctcaag
     1141 atgatgttag acaagaagcc gctaaaaatg gatggttaac aatttatcct gaatttaatc
     1201 aaaactatac cgaagtaaaa tcacaacagt ttaatggaac agcaaaagtt gatttgttcc
     1261 ctgatttgac tattgatctt actgctgacc gtaattatat ggaaagttat tctgaacaat
     1321 atgacgtttt agatggagtt tataattcgc gttcgcccta tacaatggga aatttttcaa
     1381 tttcaactgt tttaatttca acttctttta gtaaaagtga tgaatttggc tcctctgctt
     1441 ttcaagattt tagagataac aggttggtga tagcaaatcg attggctgct gatttttatg
     1501 gggttaatat tcctagatac ggtgatgcta ataacccaat tccatccgat ccggcagacc
     1561 caaaatacaa cttctattct tctaatgccg gatatccaat tggatacgga aaaaataatc
     1621 aagctgtatt aattccttct ttcttatctg cttattcagg gaaagatgaa aacagcactt
     1681 cgttaagtac atttagagat tttccaattc caaattggac cgtaaaatat aacggtttga
     1741 tgcgatatac tttctttaaa gaacgattta aaagattttc actgcaacat agttacagag
     1801 cggcttatac aattaattcc ttccggtcaa attttgaata tgataaaaat ccgggcggac
     1861 aggataatga aggttttgga aatttttata accaaacgat tgtgcaaaat gttaacttaa
     1921 tggagcaatt caatccatta atccgaatgg attttgagat gaaaaattca tttaaaatgt
     1981 tagctgaaat gaagaaagac cgtgcattat caatgagttt tgacaataat ctacttactg
     2041 aagtgcaagg tttagaatat attatcgggt taggctaccg attaaaagat gtagtaatta
     2101 cctcttcatt agcagataat ccacaaggca taattaaaag tgatatcaat ttcagaacag
     2161 atttctctta tagaaataac aaaacaatag ttcgttacct agattacgat aataatgaac
     2221 ttgccggagg tcaaaatatt tggtctgtta aattaactgc cgattattct ttcagtaaaa
     2281 atttgacagc catattttat tatgatcata ctttttctaa agcagtgatt tctacggcat
     2341 ttcctttaac aaatatcagg tctggtttta cacttcgata taattttgga aattaattaa
     2401 aatttcaaca aaaaagattt tataatcaca gcataaaaat tacatttgct gaaacaaatt
     2461 acaaaatgaa tataccaaca aatttaaaat acacaaaaga ccacgagtgg gtgagtttag
     2521 atggtgaaat tgctacggta ggaattacag attttgctca aaaagaatta ggagatattg
     2581 tctatgttga agtggaaaca ttagaccaat cattagaaaa agacgaggtc tttggaacgg
     2641 ttgaagcggt taaaactgtt tcggatttat ttttacctgt ttcaggtgaa atcattgaat
     2701 ttaatgaaga cttagaatct aatcctgaaa gtgtaaatac agatccttat ggaaaaggat
     2761 ggatggtgaa agtaaaagta tcaaatgttg atgattttga tgatttatta gatagtcaag
     2821 cttataaaga gttgattggt gcctaaaaaa aagatattac tatttttatt agttctttgg
     2881 ttggtttttg ttaccacgtt aagtctggta agtttgaata gtttttctaa agtacaaatc
     2941 aaaggcggag acaaatacgt tcattttatc ttttattttg ttttaacagg gttattaatt
     3001 ttgaatttaa tgtcaaaatt taattttaga aaagcagttt ttatttnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnttat taattttgaa tttaatgtca aaatttaatt
     3181 ttagaaaagc agtttttatt tctgctattt ctgctgttat ttatggcata attattgaag
     3241 tattacaagg ggttgttaca acctatagag agcctgaaat attagatatt ttatttaatt
     3301 cacttgggtg tatttttgca gcattcctga tgattaaaaa ccatgaaagg ttaaatttta
     3361 taaaataatt ttaaagtttt ttttaattag ctatattagc atctcaaatt acatattatg
     3421 gaactaaaaa agaatccaaa tgttgaccca aaaagaaata gttcactcta tttccaatta
     3481 ggattggcgg caattgttgc cttgtcttat atggcaattg aattgaaaac atacgaaaaa
     3541 gatttgtccg ggctaaccca aaaagttgac aatagttttt tagatgaaga agtgcctatc
     3601 actcaacaat tgaatacgcc tccaccacct ccaccacctc caccaccagc tgctccggaa
     3661 gttattaaag tggttgaaga taaaaaagag atcaaagaaa tgaacattca atctacagaa
     3721 acaaaccaac aagaagttgt tgtgaaagct gaaaaagttg ttgtggtaga agaagaagtt
     3781 gaagttgatg tgccatttgc aattattgaa gatgtgccct tattccctgg ttgtgaaggt
     3841 gtagcaaaag acaaaagaaa agattgtttt caagagaaaa tacaagctca cattagaaaa
     3901 aatttccaat acccggaagc tgctttagat gctggacatc aaggtaaagt attcgtgagt
     3961 tttgtaattg aaaaagacgg aagtgtaact attgcaaaca tgagagcacc ttatccaaca
     4021 ttggaaaaag aagctcaacg tatcatgtct aaattgccaa aaatgattcc tggaaaacaa
     4081 agaggtaaac cggtaaggat gacaatgagt attccaatca catttaaatt agaccaataa
     4141 aataataaaa atagataaac ttaaatccac tcgaactcga gtggattttt ttttggcaca
     4201 aaaattggat tgcattaaaa ataactaatc cattttaacc atgagaaatt tagatcaata
     4261 cccacgcgta aaccacaaca gcaaacttta ttttcaactt ggacttattg ctagtttaat
     4321 tgctgtatat tttgttatcg aactaaaatc gccaatttct gagtttgtag ttgagtcaaa
     4381 aaaagaggta gtagatattt cagaaaaacc ttttcatgag acctttcgga ttgaaaaaga
     4441 atttaaggaa gtggttgaaa aagtaaaaat agataaagct cctagtaaac caatcttaaa
     4501 taggtttaag gaggttccga atacaaccat tgagcctata caaacggatg ttacttcacc
     4561 ggatgatata gaagacaatg aattgctttt gaaacctatt gaaacaacag ctgttgtgtt
     4621 aattccaaca gaaaataaaa ttattgatcg atgggctttg gaagaagtcc cagcttttaa
     4681 ggcttgtgct aaattaaaag gacctgcaag agaaacctgt tttaatgagc aaatggctaa
     4741 ctttttaatt tctaatctgg aatatcctga acaggccaaa gaaaatggag ctcaagaatc
     4801 gatattggta gaatttataa ttaataagga tggttcagtt tctaatgtta aacccgtatt
     4861 tgaagaaaaa gtaatgaata aagagcttga aaaagaagcg ttacgggttg tcaaaaaatt
     4921 gccaaaattt gagcccggga aacaagccgg acaaccggtt agagtaagat ataccatacc
     4981 aatcactttt aaaattaaaa aataaaatat ttaagtataa aaagcatccc acttcggtgg
     5041 gattttttat ttttacacta tggatattaa acaattttta gattcgacct atttaaagac
     5101 accaactcaa gcagggttgt cttttgaaga aaataaaaaa atagttgaag gttttatagc
     5161 cgaagctatc gaagaacgtt ttaaattaat tatgattcga ccggatatgg tttctttagc
     5221 aaaaaaaatg attgctaaag ctgaatctaa agttctgatt ggaacagtta ttggttttcc
     5281 ggaaggaatt tatactattg aagaaaaatt agcggaagcg actcaagcca tagaagacgg
     5341 agcagacgaa ttagattatg tttgcaacta tgaaaatttt aaaaatggcg atagtcaata
     5401 tgttaaagaa gaaattttaa aatgtacaca actcggtatc agtcaccaca aaactgtaaa
     5461 atggattatt gaagtggcgg cattaaatga ccaacaaata attcaactct caacacttat
     5521 caaaaatgta gtgatagcta actttaaaga agagcagtat caagatgtat ttgtgaaatc
     5581 atccaccggg ttttataaaa cggaagccgg aaaaccaaat ggtgcaactg ttccaactat
     5641 cattatgatg ttagaaaaca gctctccact tcccgtgaaa gcggcaggag gagtaagaac
     5701 ctatgaggaa gcaatcgaaa tgatttcttt aggtgtaaaa cgaattggta catcttctgc
     5761 aaaagcaatt gctcatggaa acatttcaga aagtaattat taaaaattga attcatttaa
     5821 atgaaaaaat atagtaagta tttcgcctta gttctatttt ctgtttccct ttggtcacag
     5881 gtcaaaagca cagaacagtt tcctgttttt ccaacttgtg aaggtaaatt taatatagaa
     5941 ttagaaaatt gtttttatgc tgaagtacaa caaaatattt tttctaaatt caaagttcct
     6001 gataaaattt tagaaaagga ttacaaaggc agtttttttg tgttgtttga agtaaataat
     6061 caaggaaaat ttatattgca atatgcaaat gcccctttca ctgaattaaa tgatgaatgc
     6121 aagcgggttt ttaatgaaat gcctcaaatt gcaccgccta cctatggtgg taacccaacc
     6181 tatgcaaggt attctattaa aatttcaatt cctttaaaag atgctaattc catttcaaat
     6241 gttctctcag aaaataatga aaatttaaac actgttgttg ttgacgaaaa aagcaaacaa
     6301 ctaaatgaat tagacagtat tcgctactca aagtttgaaa atcctcagtt taagagcaat
     6361 ttaaacattc ctttttcaca tagtttttat gcccagtttg ataaggagat gaatcaattg
     6421 ggagccaata atcacacagc atcaaaacca ttaacttatg cagaagtaaa tcaatattat
     6481 gatttggcac aagcgaatca aaaattgatg aaaaataaaa caggctggtg gggtagaaaa
     6541 ttatggaatg aatacattct tgaaatacaa ggggataact attggtttac attaaatcca
     6601 atatgggatt taagagtagg gaaaagtaac ccaagtgttt cggattatac ctatcaaaat
     6661 accagaggaa ttcaaatcca gggaggaatt ggaaaagaag tgaattttac aacatcgata
     6721 tacgaaagtc aggggcgttt cgcagattac tataataatt atgccgaaag tattaagcct
     6781 tccggaggaa atcctgccat tattcccggt attggaattg ctaaagagtt taaaacagat
     6841 tcctatgatt ttcctttagc agatgcaaat attacctata caccaagtaa gttttttgat
     6901 ttccaattag gatatggacg aaattttata ggtgatggtt accgatcgct ttttacaagc
     6961 gatggagcca gcccatatcc ctattttaaa attaatacca aattttggaa aattaaatat
     7021 accaatacgt atatgtggtt gaaagatgtt actccggaag ccaccgtgga cggaacttat
     7081 gcctctaaat atatggccaa tcattattta agttggaatg tttccaatcg tttgaatata
     7141 agtttttttg aatcagttgt ttggacagat acgaatggac gtggctttga ttttagtttt
     7201 gtcaatccaa ttatttttta tcgttctgtt gaatttaatt catcctccaa aacaggaaat
     7261 gctttgttgg ggattgcatc aaaatataag tggaataacc atatcaatct ttacggtcaa
     7321 ttcctgattg atgaattttc tgtaagtgat gtgaaagcag caaatcaaag ttggagaaat
     7381 aaatttggat atcaaatagg agtaaaatat tttaatgctt ttaaggtgga taatttactt
     7441 ttccaactag aatacaacca tattcgacct tatgtgtatt ctcatagcac acctattact
     7501 aattatggtc ataataatca gagtttaggt caccaatggg gtggaaatgc gaaagaatta
     7561 ttagcaattg caagatatca tcaaggcaga tggtttggtg atttaaaact gacttacggc
     7621 gtgagaggat tagattttga cacccctgaa gatgataaca attacggagg aaatatttat
     7681 aaaagttata acgagaaccg tccttttgac actgatgtaa aagtgggtca agggaatgag
     7741 acaaccattt ttatcgccga ttttcaagcc ggttatttaa ttaatccgac aaataattta
     7801 aaactttttg gcagcttaat ctacagaaat tttgacccaa ctcaaaatac cttgacaact
     7861 tttaaagaaa cgaccacttg gttttctgta ggagtaagag ctgatttatt taatttttat
     7921 tttgattatt aaacagaaat tttcaagtta atttttaaca tctattttat aaattgttag
     7981 attctattta aagtttctaa tttgtatctt tgacgcagtt tttaaaatta agaaaaccgc
     8041 attgaataca attcaaaatt cttttactgc caaatcgctt tatgttgatt ttaaagaaat
     8101 cactaaagca ggtttatctg ttagtgttgt tttttcatct attgccggat attttcttgg
     8161 aatattggac atttcatcat tcagttggat aactttgttt atgctggctg ttggaggata
     8221 ttgtatggtt ggagcttcca atgcattcaa tcaaatcatc gaaaaagatt tggataaatt
     8281 gatggataga acaaaaaaca gacctatcgc agcaggaaga atttcagtca ataccgggtt
     8341 tataattgct tctgctctta ctttacttgg cttaattatt ctatataata tcaacccgaa
     8401 aacggccatg tttggagcgg tctccatttt tttatataca agtatttata cccctttaaa
     8461 aaccgttacg ccactttctg tttttgtggg agcaataccg ggagcaatac cgtttatgtt
     8521 gggttgggtt gccgctacag atgagttcag cttagaagca ggaacattat ttttaattca
     8581 gtttttttgg cagtttccac atttttgggc aattggctgg tttttgtttg atgattataa
     8641 aaaagccggg ttttttatgt taccaaccgg taaaaaagat aaagggacag ccttacaaac
     8701 tattttatat accatttggc tgattattgc atctttatta cccgcaattt ttccttatat
     8761 aggcatagta ggaagtcgat tatatttgtc gccaatttca gcaattattg tatttttgct
     8821 cggaatttgg atgttgtatt acgcttttga gttatataaa aaaagagaca gtaaagccgc
     8881 ccgaaaatta atgttggtaa gtgtaagtta tattaccctt cttcaactag tttatattgc
     8941 tgataaattt ttaagataat tatggaaatg acaagtcagg aacacctaga acgaaaagga
     9001 caatcctata aattgttgtt gtggtttgcg atgattagta tggttatgat gttcgccggt
     9061 ttaaccagtg cctatgtagt aagctcaagt agaaaagatt ggttaaaaga tttcgaaatg
     9121 cctgctgctt ttatcattag cacattagta attattttga gtagcatcac ttttcattta
     9181 gccggaaaat caattaaggc aaataacaga agtgtaacaa caatttggct tttatccact
     9241 ttaggattag gtattttatt tgtagtattc caatttttag gattcaatca aatcattcaa
     9301 aacggttatt tttttaccgg tagcgagagt aatgttacga cttcatttct ttacattgtt
     9361 gtaattcttc acttagctca cctttttgga gggctgattg cacttttaat tataatttat
     9421 aatcatttta aacaaaaata caattcgact caaacccttg gaattgagct aggtgcgatg
     9481 ttttggcatt ttcttgatat cttatggata ttgttgtttt tatttttcta tttttacgaa
     9541 tagaaaaaaa atgtaaattt gggaactttt taactaataa ctatatatgg gagcgacagt
     9601 tacgacaact gaagaaaaaa tctggaatgg tggcaatgag ccaatgaaag ccagttatgg
     9661 taaattgatg atgtggtttt tcattttatc agatgccttg acctttacag gatttcttgc
     9721 agcttatggt ttttcaagat ttaaatttat tgatagctgg ccaattgccg atgaagtgtt
     9781 taatcacttt ccgtttttac acggacaaga tttgccaatg ctttatgtag ctttgatgac
     9841 ttttgtattg attttttctt ctgtaacaat ggtgttagca gtagatgcgg gtcatcatat
     9901 gcaaaaatca aaggttgctt tttacatgtt tttaaccgta ttgggtggtt taatattcgt
     9961 tggctctcaa gcttgggaat ggaaaaactt tatcaaagga gaatatggtg caattgaaac
    10021 aaaagggggt agcgttatac aatttgttga taaagaggga aaacgaatca aaattgccga
    10081 ttttgcgttg acacttccgg aagatcgtgt tcaacatgaa cgtaaaaatg gaatttggtt
    10141 ttcaaaagat gaatcaagtt tacctaccta ttccgtgaac gaagtagtgg aaggttttaa
    10201 agctaatcca aatattttgg ttagaactca agtaacttat catgacaaaa atgataaaaa
    10261 aggtgttcat ccagatttaa ataaagaaaa gcataaaacc attttaacca gagaagaatc
    10321 gttagcccga ttaaataatg cagtttatgt gattgaagga gctaatttga ttagaaatga
    10381 atatggaaat aaacaatttg caaacttttt cttttttatt accggtttcc atggttttca
    10441 cgtattttca ggagtcgtaa ttaattttat tattttctta aatgtaattt taggaactta
    10501 tgagaaaaga aaaaattatg aaatggtaga aaaagttggg ttatattggc actttgtgga
    10561 tttagtttgg gtatttgtat ttaccttctt ctacttagta taatttttta aaaaagtata
    10621 aaaatggcac acgcacataa tttagcaatt ttcagaggaa ctttaaaatt caaatcaaac
    10681 gaacaaaaga tttggggtgt attggttttc ctttctatgg taacagctgt tgaggttgtt
    10741 ttagggattt ataaaccttc ttttttaatg actccattta tgactccgtt tgaaggagga
    10801 ttaggagcga cattagtaaa catattttta tctccattca tatttttaat tccgttaaat
    10861 ttaatattct tgtttttaac gattgtaaaa gcgtattaca ttacttggga ttttatgcac
    10921 atgcgtgacg aaagaacatc tttaaaaaga gccgtagttt ggacgtttgt atttcttgca
    10981 ttatatatcg cagtgttgtt tttgacagaa gcagattata tttatgaagt ttatagaact
    11041 tccacaatta agtggaattt ttaagatgat atttattcaa ttttaaggta cgcattgcgt
    11101 accttttttt atttttgtaa agcaaaaaaa atgcaaacgc aatgaaaaag aatatagtac
    11161 tttttgtttt atttatttta ccgattgtag cttatctttt ttttgcttcc ggagttaaca
    11221 attttgccaa attgcctacg gttactgcta atatacctga tttagggaat tggaagacaa
    11281 ctaaagggga acagcttaca ttggatgaga aaattacagt aattgctttt ccgggtacag
    11341 aaattctcta taataaagga gatgccttta atttaaatca aaaaatatat aataaagttc
    11401 gtgagttcaa agactttcaa ttggttatga ttgcgcctat aggaacagaa tctcaaacgg
    11461 aaacattaaa agaacaatta gcatccatat cagacttatc aaaatggcat tatgttttcg
    11521 ccaaaccgga agaaatacaa tcctattata ataatcttaa tttaattgga caattggata
    11581 agaatctaag aactcccaat gtttttattg tggataaaaa acgtaattta aggggaagaa
    11641 aaggaaaaaa taaaaaagga gaagcggaat ataaagaagg gtataattca atttccgcag
    11701 ccgatttaca caatgaaatg ggcgatgaca tcaaaataat cttagctgaa tatcgcttgg
    11761 ctctcaagaa aaacagtgca gaaagaatta aataataaaa atgaaaaata aatcatatat
    11821 cggaatttct tttatcattt tagttttcgg aattattttt attcctaaaa ttatcgatag
    11881 aattaaaaat aatgatgtca ccaaaattga caggttaaat acgtccggtc aaagtgaaac
    11941 ttcggaagat ttgataatga aaattgggcc ggctccaaaa ttttctttga caaatcaaaa
    12001 tggagaaaca attaccaatg ataattataa aggaaaagta tatttggtcg aatttttctt
    12061 ttctacctgc ccaactattt gtccaattat gaatcaaaac atggttaaaa ttcaaaatca
    12121 ttttgaagga aaaactgatt tcggaatcgt ttccgttacc atcaatcctg aaaatgatac
    12181 ttcagaagtt ttaaaaatac atgcagaaca tttaggggta aaaaatccaa attggaattt
    12241 tctaacgggg gataaaaaat atatttttga tttggccaac aaaggattta acatatatgc
    12301 cggggaaaat aaagatattg atggtggatt tgaacattcc ggtttatttg ccttaattga
    12361 taaaaaaggt aatataagat gccgtaaaga taaatttggc aatccaattg tatattatga
    12421 tggattagaa gaaacaggtg tccaagcttt acaacaagat attaaagtac ttttagacga
    12481 gaaataatat ggataaacaa ttaaattctg aacaaccaaa cacaattgaa aataagttca
    12541 ataaactgat tataattgta tcaatagcca tacccgttgt tgtggctatt ttatttggca
    12601 ttaaacttaa agattatgga ataaatgttg agccattaac tttcttgcct ccaatatatg
    12661 ccgttataaa tggtgtgaca gccatgttgt tgattcttgg ggtttattct attaaaaatg
    12721 gaaataaaaa agcacatcaa aattttatgc aggccgctat tgcttgttcg gttgtttttt
    12781 tagtaatgta tgttgcttat cacatgactt ctgatagtgc catttatggt ggagtcggtt
    12841 ttattcgata tgtttatttt tttattttaa tttctcatat tattttatca attgctgtta
    12901 ttccgttagt attaattacg tatgtaaaag ctttagcgga acgttttgat aaacataaaa
    12961 aaatagcaaa aataactttc ccaatctgga tgtatgttgc agtcaccgga gtaatagttt
    13021 atttgatgat agctccatat tatgcatatt aaaaagttaa aatattacct tgcgatttta
    13081 ctgtttttag ctgagttttc agcctcagcc caatgtgcaa tgtgtcgagc cgctcttgaa
    13141 agtgaagaag ggggtaacaa agccgaagct attaacaatg gaattgtcta tctgatggct
    13201 tttccttaca tcatggttgc gattgttttt tatgtagtat atcgcatgaa gtttaaaaag
    13261 aaaatagaaa gcgaagcatt ataaattttt actccatccc atcaattttt acatcaacct
    13321 gaccactggt tttaataaat gctaagatag ctttgtttgt aagttcaatt ctttgaaatt
    13381 caaaatcggc taaagtacca tttacaaaaa cacctttcat aggggaatag ttagtgaggt
    13441 attgcatcac attctgtttg ccctcctcta agttgggttt aatggaatag cgacaacttt
    13501 cctgaatttt cctcaacaca tatccttgag ctaaccaatt ggcagaacgt atcaaaacac
    13561 ttttagtatc tagaacatag tccaatttgt caaaatagat ttcttttgtt tcttcgttat
    13621 attgaggaaa tcctgataaa taaatagttc cgtttatact gccaagaata tccaacgcaa
    13681 caatcatttt accatttttg tgccatagat ttacattggt aactttaact tttctctttc
    13741 catcaccaaa ttcctgtccc tggaaatttt tagtcataat tatggaagca tcttcatagg
    13801 tggaaactgc aattaggttt gctgtaattt gatcaggcat ttttgctacc ggtttcaata
    13861 ctattttact tttatcgaat tttttttccg gttttttccc aataatagtt tccatggaac
    13921 atttcaatcc catatccata gcaatagcat cttttttcaa aatagcatca gttacatata
    13981 attcaatagg tacaattctg agccaacttg catattcttc actcatttca aaaggagtag
    14041 caattttgtc caaagcttct aaaacttgag gtttgaaatt caaagactct tttattgaat
    14101 catcaatgcc cttttcaatt tttgatttaa aaattcgaat ggttgggttg attaaatagg
    14161 taattggaat attttttccg gcgatagtaa ctgttggact ttctgtccaa tctaacgatt
    14221 taattttagt attggttttg agttgccaat tggttaaatc aacttcacta agaagagtga
    14281 caataccatt caagttaaat tctttggtgt catataattt gatgcccatt ttatcaaatc
    14341 catagcgata agtagcattg atttttaagg gcaaagtagt tttaatttta gtgatattgc
    14401 cctctttttc actctctatt tttataggtg cttgtttcca aactctcata ataatgtcgt
    14461 catcattcat gatgctgtca ttgtaaatca tcccatttaa aagcttgttg gtttgatttt
    14521 caacatcttt tagcttgata gatacgggaa gttttaaaaa tgatgtggtt gtttcataaa
    14581 ccaaaggagc agcattatca ggttccggtt taatgatttc taatttttta gcggtagaac
    14641 aggccgaaaa tgctactaca atcaggatta gaacaagtgg gaaaatcaaa tatttcattt
    14701 caaaaaattc atttttagac cgtaaatgta ctttatttta agattttttc attttgttgt
    14761 aacacaaaat tagttttagc gtcctatcgg tataaaattc aaaagcatta aatttgtatc
    14821 accaagcaa
//
LOCUS       DAOTCW010000056        14157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2547_length_14157_cov_50.883634, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000056 DAOTCW010000000
VERSION     DAOTCW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14157
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2547_length_14157_cov_50.883634"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1375
                     /gene="ricT"
                     /locus_tag="P5335_06190"
     CDS             197..1375
                     /gene="ricT"
                     /locus_tag="P5335_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRZ74501.1"
                     /translation="MACTNCSTGSKDGGAPKGCQNKGTCGTDSCNKLTVFDWLSNMSL
                     PSGQEPFDCVEIRFKNGRKEFYRNTEKLTLSIGDIVATEASPGHDVGIITLTGELVKI
                     QMKKKGVNHTSPDITKVYRKASQKDIDIWKDMQAKEESMKVKAREIAIQLQLQMKISD
                     IEYQGDGSKATFYYTAEDRVDFRQLIKEYAQTFKARIEMKQVGFRQEAARLGGIGSCG
                     RELCCSTWLTDFRSVNTSAARYQQLSLNPQKLAGQCGKLKCCLNYELDTYMDALKDFP
                     DFETKLETEKGVAYCQKQDIFKGLMWYAYADNIANWHVLHVEQIKEIVAENKQKRRVA
                     SLEDFALEIVQEPKQDFNNAMGQESLTRFDQPKKKKNNNNNNNKKRRPNNIIRNDSKK
                     "
     gene            1359..1844
                     /locus_tag="P5335_06195"
     CDS             1359..1844
                     /locus_tag="P5335_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HRZ74502.1"
                     /translation="MTLKNNFFLVVLLLFLVSCDKKRVFDEYKTVGQNWHKDSIVSFT
                     FEQKDTLTKQNLFINIRNNKNYPFNNLFLIVEMEHPSKKTLVDTLEYKMAKPDGTLLG
                     EGLSDTKENKLFYKENFVFKNSGQYKVIIQQAVRQSGKIEGIEKLDGITEVGFRIEKQ
                     E"
     assembly_gap    1858..1957
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2008..4287
                     /locus_tag="P5335_06200"
     CDS             2008..4287
                     /locus_tag="P5335_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRZ74503.1"
                     /translation="MAQKKYTGVKDIAYYQKKFWKIFFLGLGGVFLFFVFANFGLFGS
                     MPSFDELENPDSNLATEIIASDGVILGKFYRENRSPVKFEDLPKHLVDALISTEDERF
                     YEHSGIDAKRTFGAAVKLGADGGASTISQQLAKLLFHGEGSRFILFRMVQKVKEWIIA
                     TKLERQYTKNEIIALYLNRADFVNTAVGIRSAAKVYFGKEPRDLTIEEGALLIGMLKN
                     PSLFNPVREKRKQLVLDRRNTVLAQMVRNNFLEPEAKAILEKKPIVLDFNPESHTDGI
                     ATYFREYLRDYMKKWCKENKKPDGTEYNVYNDGLKIYTTIDSRMQKHAEEAVDEHLKN
                     LQKEFFKAQKDNKNAPFLKLNKDETDKLLMRAMKVSERWRLMKLQEKSEEEIIKSFSV
                     KTDMRIFTWKGERDTIMSPMDSIRYYKHFLQTGMISVEPQTGHVKVWVGGINYKHFQY
                     DHAGQGARQVGSTFKPFVYATAIEQLNMSPCDSIIDSPFTIPRGRHNVTESWTPRNSD
                     GKYRGMITLKKALANSINTVSAKLIDRTGPRAVIDLTHKLGVTTEIPEQPAICLGAVE
                     ITVQDMVAAFSTFANKGIYIKPQLITRIEDKNGNVIFESIPETHDVLNQDIAYAVIKL
                     MEGVTEDGTGTRLRWGGAGFTKYNYQFTNPIAGKTGTTQNNSDGWFMGIVPNLCTGVW
                     VGCEDRSARFRSTSMGQGATMALPIWGLYMDKCYKDDKLNVSKEAFDRPKNISIKVDC
                     WKPVKDTLDFDQEVDEFNF"
     gene            4375..5076
                     /locus_tag="P5335_06205"
     CDS             4375..5076
                     /locus_tag="P5335_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRZ74504.1"
                     /translation="MITRKVNSVQEALSGITDNMTIMLGGFGLCGIPENSIAELVKLG
                     ISDLTCISNNAGVDDFGLGLLLQKKQIKKMISSYVGENAEFERQMLSGELDVELTPQG
                     TLAEKCRAAQHGIPAFFTPAGYGTEVAEGKETREFNGKMYVMEKAYKADFAIVKAWKG
                     DEAGNLIFKGTARNFNSCMAGAAKITIAEVEELVPAGTLDPNQIHIPGIMVNRIFKGE
                     KFEKRIEQRTVRQKA"
     gene            5080..5739
                     /locus_tag="P5335_06210"
     CDS             5080..5739
                     /locus_tag="P5335_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRZ74505.1"
                     /translation="MALDKNQIAKRIAKEVKDGYYVNLGIGIPTLVANFVRTDISVEF
                     QSENGVLGMGPFPFEGEEDADIINAGKQTITTLPGASFFDSAFSFGMIRSKKVDLTIL
                     GAMEVSEKGDIANWKIPGKMVKGMGGAMDLVASAENIIVAMMHVNKAGESKVLKNCTL
                     PLTGVGCVKKIVTELAVMDVTPNGFKLLERAPGVTVEEIIKATEGKLIVEGEIPEMIL
                     D"
     gene            complement(5791..>6117)
                     /locus_tag="P5335_06215"
     CDS             complement(5791..>6117)
                     /locus_tag="P5335_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74506.1"
                     /translation="TFAKLTFAKTIGKTELKNIFIRPILEENELKLELKFRFQKEELF
                     EIHSVGSAFNRLLPFINNPFLSVILFTTEFDLTYKLNKKRVVSVTDQHPTFGNANEVL
                     LAYLNK"
     assembly_gap    6118..6217
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6218..7605)
                     /locus_tag="P5335_06220"
     CDS             complement(<6218..7605)
                     /locus_tag="P5335_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRZ74507.1"
                     /translation="MNNQKLLSVENLTISFKREKEFIPVVHQLTYELYENEILGIVGE
                     SGSGKSVSSLAIMGLLPSSISKIANGSIVFEGKDISLLSDQEFQKIRGNEIAMIFQEP
                     MSSLNPSMKCGMQVAEILEIHTSLSKKEIKNQVISLFEKVKLPNPEELFSKYPHQISG
                     GQKQRVMIAMAIACKPKILIADEPTTALDVTVQKEIIQLLKELQRETKMSILFISHDL
                     SLVSEIANRVLVMYKGQIVEQGIAKEIFENPQHNYTKALIASRPSLDFRLKRLPTIQD
                     FLKESFKSEIVSAEERNINQLKIYSKSPLLEVRNIEKDYFSSVGLFGKQHSFKAVNEV
                     SFKIYEGETVGLVGESGCGKSTLGNAILQLDKATSGQIFYKGNDITNLTKEYIRKLRK
                     EIQIIFQDPYSSLNPRIPIGKAIMEPMKVHGLFANDAERKAKTIEILERVGLGESFFN
                     RYPHEFSGGQRQR"
     gene            7762..>8528
                     /locus_tag="P5335_06225"
     CDS             7762..>8528
                     /locus_tag="P5335_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8"
                     /protein_id="HRZ74508.1"
                     /translation="MKNIYFSGKIIVLIMIVFTTPFLLKGQTVEQKKALLEQYDIAKI
                     KTLQVQFAKKAKTEKEKAILAARANQWEIFKFNADGSFDELIGLHPDGSPKYFSINNV
                     NAAKSTRTNHLNSGGSLGLNLNGQNMIGGVWDGGPVRISHQEFGGRVQIGDGETVLNS
                     NSFHGTHVTGTITATGVQANAKGMANLATVKTFDWSNDEAEVLAEIQNGLLLSNHSYG
                     VPISSAPGPWYIGAYSADTRAWDEIQYVSPYYLMVVSA"
     assembly_gap    8529..8628
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8629..10737
                     /locus_tag="P5335_06230"
     CDS             <8629..10737
                     /locus_tag="P5335_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ74509.1"
                     /translation="GNAGPDAKFGWGLLNAKAAAIAITNNGLNTWISEEKIATNETYT
                     MTVKSIAGAPLTATICWTDVPGIVNNGILNSSIPALVNDLDIRITQGSNTYFPWRLQS
                     NASLLAVRNGDNFVDTVETIKIDSANGGDYTITISSKGTLVNNDQPFGLIISGIDSSF
                     GIVPLGYDQVKCANEEAVFSFNFNGASSGNVNLSAINIPAGANVNFSSNPINMNGIFT
                     MTVSNLSNVIPGEYSIGVVGSNGLDSETRYVNLKILSNQFNSVTTTYPINGQTGLSTT
                     LNLTWEEDINAENYMLEIATDFNFNSIIESQSTANTNYTATNLASETVYYWRVKPSNR
                     CGTSTNYSVSSFQTGILNCQNQFIGTDFSDASIGSVANSLASVPVTVSGGFVIGDINV
                     TLNLTHTWVQDMTIYLDGPASIGSPRITLFEEPCGGEDDIDAILDDSGLDLECGINPA
                     PAISGIIKPLQLLSNLNSLPADGIWKLQVIDNYNGDGGSINSFSLNFCSLSTVTNNLN
                     FENNGIVTTTSSNKVILNTEINASTPSQSDNNQTYTLTETPILGLLTKNGTALTIGNT
                     ITQSDINEGRISYSNSQFEPGQDSFKADILNSSSGWLPNNTVIITIENSLSTSENLME
                     FVRIYPNPTQNYITIQLTNEIVGETKMDLFDVQGRKIISKNTFQNTEELNLSSLSGGI
                     YFLSIQNKNHKIIKKIILKK"
     gene            complement(10762..11475)
                     /locus_tag="P5335_06235"
     CDS             complement(10762..11475)
                     /locus_tag="P5335_06235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRZ74510.1"
                     /translation="MIEKIKLDNILFLDIETVPEEENFKLLDEEKQYLFETKTQYQRK
                     DEFTPEEFYDKAGIWAEFGKIVCISVGYFITKGDVRNFRVTSFFGEEKKILKDFSNLL
                     NNHFNQPQHILCGHNAKEFDFPFIARRMIINQIEIPAKLNLFGKKPWEVPHLDTLELW
                     KFGDYKHYTSMRLLTKVLQIPSPKDDIDGSEVAYVYYVEKDIDRIITYCEKDVIAVAQ
                     VFLRLRREDLLIEEEIIHV"
     gene            complement(11621..12076)
                     /locus_tag="P5335_06240"
     CDS             complement(11621..12076)
                     /locus_tag="P5335_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRZ74511.1"
                     /translation="MKSLKVSLFAFAATVLFSVSANAQEKKAAVTPAITTTPAPQKPT
                     TPVKWKEEIFDFGDIKKGTPVSHDFTFVNTTKQTVLVTNVKASCGCTATNYTKTPIKP
                     GEKATITATYNAASPGAFSKTVTVSLNENEVPKILTIKGKVLDDAPVTQ"
     gene            12240..13508
                     /locus_tag="P5335_06245"
     CDS             12240..13508
                     /locus_tag="P5335_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRZ74512.1"
                     /translation="MKFNRLNSIIFAGFLSIVGVLIMQLLMLNRAYSFEKKEFEDKIH
                     FALQDVVKRIYRDNKTDLAITNQIKKITDDYYIVNIDDVFEHQVLEYYLKSEFQKVKL
                     GIDYEYAIYNCGTDEMIYGNYVSSDSGKPEKCENCFQKNEGLIYYFGIRFPDLQHKFI
                     TSLGQYWIYTGILFLVLIIYVYSVFLLLKQKKYTELQTDFINNMTHEFKTPLSSILIA
                     SQYASSQDEIKNNPKLAKYMQIITNQSHKLNQHIERILNVAKGDAKWIELDKKPINIK
                     DALELVKENAILKSTKKSVVKIEIPSKISVLADEFHFNNVLYNIVDNALKYCSESPEI
                     VIKSVENDRGITLHFSDNGSGIAPQHIDYVFDKFYRVPRENKKDIEGFGIGLFYVKKI
                     IDLHHWKIAIQNNENQGITVFIFIPKKDVV"
     gene            13505..>14157
                     /locus_tag="P5335_06250"
     CDS             13505..>14157
                     /locus_tag="P5335_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRZ74513.1"
                     /translation="MSKKKILYVEDDESLGFLTADSLENQYDIAHFINGKEGFDAFCS
                     NEYDLCILDVMLPDMDGFEIATAIRKRNLEIPIIFLSAKTMKEDRIKGLKLGADDYLV
                     KPYSMEELTLKIEVFLNRSQKTISKTTTTYLIGPFEFDPTNYCVSKGKEVTNLTEREA
                     ALLKFFLENKNTVLKREKILMELWGTDDYFVGRSLDVFISRLRKIFKEEPNIRIENIP
                     "
BASE COUNT         4885 a         2117 c         2458 g         4397 t
ORIGIN      
        1 aagcttgtga agctgccgaa aaggcttgtc cggttcatat tatttcggta aaagagacgt
       61 aaactagtat aattttgttt gttttgattt tgaaattgga agcttaaact tctatcaaaa
      121 aaatactatc tttacacact taacgtttaa tgtcagtagt ttcgatagaa tcggaatgac
      181 caatatataa tcaattatgg cttgtacaaa ctgttctacc gggagtaaag atggaggagc
      241 tcccaaagga tgtcaaaata aagggacttg cggcaccgat agctgcaata aattaacggt
      301 tttcgattgg ctttcaaaca tgagtttgcc aagcggtcaa gagccttttg attgtgtcga
      361 aattcgtttc aaaaacggac ggaaagaatt ttatcgcaat actgaaaaac ttaccttaag
      421 cattggtgac atagtagcca ccgaagcttc tccgggtcat gatgttggca tcataacctt
      481 aaccggcgag ttggtaaaaa ttcaaatgaa gaaaaaaggg gtgaatcaca ctagtccgga
      541 cattaccaaa gtataccgaa aagcatcaca aaaagatatt gatatctgga aagatatgca
      601 ggcaaaagaa gagtcgatga aagtgaaagc acgtgaaatt gctattcaac ttcagttgca
      661 aatgaaaatt tctgatattg aataccaagg tgatggttca aaagcaacct tttattatac
      721 ggcagaagat cgtgtcgatt ttcgtcaatt aatcaaagaa tatgctcaaa catttaaagc
      781 cagaattgaa atgaaacaag tgggtttccg tcaggaagcc gctcgtttag gcggaattgg
      841 ttcttgtggt agagaattgt gttgttccac ttggttaact gattttagaa gtgtaaatac
      901 ttctgccgca cgttatcaac aactttcgct aaatcctcaa aaattagccg gacaatgtgg
      961 aaaactcaaa tgttgtttaa attatgagtt ggatacgtat atggatgcgt taaaagattt
     1021 tcctgatttt gaaacaaaac tggaaacgga aaaaggagtt gcctattgcc aaaaacaaga
     1081 tatttttaaa gggttgatgt ggtatgctta tgccgataat attgctaatt ggcacgtgtt
     1141 acatgtagag caaattaaag aaatagtagc cgaaaacaaa caaaaaagaa gagtagcttc
     1201 cttagaagac tttgctcttg aaattgttca ggaaccaaaa caagatttca ataatgccat
     1261 gggccaagag agtttaacac gttttgatca gcccaagaaa aagaaaaaca ataataacaa
     1321 caataataag aaaagaagac ctaataatat cattagaaat gactctaaaa aataattttt
     1381 ttctagttgt cctacttttg tttctggttt cttgtgataa gaaacgggtt tttgatgaat
     1441 acaaaactgt gggtcaaaat tggcacaaag acagtattgt ttcttttact tttgagcaaa
     1501 aagacacctt aacgaagcaa aatcttttta tcaatatacg aaacaataaa aattatcctt
     1561 ttaataattt atttttgatt gttgaaatgg aacatccttc taaaaaaacg ttggttgaca
     1621 cccttgaata taaaatggcc aaaccggatg gaactctttt aggtgaaggt ttatcagata
     1681 ccaaagaaaa taaattgttt tataaagaaa attttgtgtt taaaaattca ggtcaatata
     1741 aagttatcat tcaacaagcg gtcagacaat ccggaaaaat tgaaggtatt gaaaaattag
     1801 acggcataac cgaagttgga tttagaatag aaaaacaaga ataaagaata ctatggcnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc ataaccgaag ttggatttag
     1981 aatagaaaaa caagaataaa gaatactatg gctcaaaaaa aatataccgg cgtaaaagac
     2041 attgcttatt atcaaaaaaa attctggaaa atcttttttt taggattagg tggtgtgttt
     2101 ttgttttttg tttttgccaa ttttggtttg tttggttcga tgccaagttt cgatgaatta
     2161 gaaaatcctg attcaaacct agctacagaa attatcgctt cagacggggt aattttaggt
     2221 aaattttatc gtgaaaatcg atcgcctgta aaatttgaag acttaccaaa acatttagtt
     2281 gatgccttaa tttcaacgga agacgaacgt ttttacgaac attcaggaat cgatgccaag
     2341 agaacttttg gtgcagcagt aaaattagga gctgatggtg gggcaagtac aatttcacaa
     2401 caattagcca aacttttgtt tcacggagaa ggttcccgtt tcattctttt cagaatggtg
     2461 caaaaagtaa aagaatggat tattgcaacc aaattagaac gccaatatac taaaaatgaa
     2521 atcattgctt tgtatttaaa ccgagcggat tttgtgaata ctgccgtagg aatacgttca
     2581 gctgcaaaag tatattttgg taaagaaccg agagatttaa ctattgaaga aggtgctctt
     2641 ttaattggaa tgcttaaaaa tccgtcttta tttaatccgg ttcgtgaaaa aagaaaacaa
     2701 ttagtattag accgaagaaa tacagtatta gcccaaatgg ttcgaaataa ctttttagag
     2761 cccgaagcga aagctatttt agagaaaaaa ccgattgttt tagattttaa tccggaaagt
     2821 cataccgatg gaattgcaac ttattttaga gaataccttc gggattatat gaaaaaatgg
     2881 tgtaaggaaa ataaaaaacc ggatggcacg gaatataatg tttacaatga cggtttaaaa
     2941 atatatacga cgatagattc ccgcatgcaa aaacacgctg aagaagcggt agatgaacat
     3001 ttgaaaaacc tacaaaaaga attttttaaa gctcaaaaag ataacaaaaa cgctccattt
     3061 ttaaaactta ataaggatga aacagacaaa cttttaatgc gagccatgaa agtatctgaa
     3121 cgttggagac ttatgaaact tcaggaaaaa agcgaggaag aaattataaa atcattttct
     3181 gtaaagaccg atatgcggat tttcacatgg aaaggtgaac gggatacaat tatgtcacca
     3241 atggattcta ttcggtatta taaacatttt ttacaaacag gaatgatttc cgtggaacct
     3301 caaaccggtc atgttaaagt ttgggttgga ggaataaatt ataaacattt tcaatatgac
     3361 catgccggac aaggtgcccg tcaagttggt tcaaccttta aaccctttgt ttatgctact
     3421 gctattgaac aattaaacat gtcgccatgt gattctatta ttgattctcc ttttacaatt
     3481 ccaagaggca gacataatgt tacggaaagt tggaccccca gaaattcaga cggaaaatac
     3541 cgaggtatga ttactttgaa aaaagcgtta gccaactcaa tcaataccgt ttcagctaaa
     3601 ttaattgacc gaaccgggcc tagagcagta attgatttga ctcataaact tggtgttaca
     3661 accgaaattc ccgaacaacc tgccatttgc cttggagctg tagaaataac cgttcaggat
     3721 atggttgctg ctttcagtac ttttgccaac aaaggaattt atatcaaacc tcaacttatt
     3781 acccgtattg aagataaaaa tggaaatgtc atttttgaat caattcctga aacacatgac
     3841 gttttaaatc aagatattgc ttatgctgta attaagttaa tggaaggagt aactgaagat
     3901 ggaaccggaa ccagattgcg ttggggcggt gccggattta caaaatataa ttatcagttt
     3961 accaacccaa ttgctggaaa aacaggtact actcaaaaca actcagatgg ttggttcatg
     4021 ggaatagttc ctaatttatg taccggcgtt tgggtaggtt gtgaagaccg ttccgctcga
     4081 tttagaagca cttccatggg gcaaggagct acaatggctc tcccgatttg ggggctttat
     4141 atggataaat gctacaaaga tgacaaactc aatgtttcta aagaagcttt tgatagacct
     4201 aaaaatattt ccataaaagt agattgttgg aaacccgtta aagacacttt agactttgat
     4261 caggaagtgg atgaatttaa tttttaaatc aatttgaatt ttaaatatac taaatgggct
     4321 ttgcgaaaag ctcatttttt ttataccttt aaaactcgaa aaacaaacaa acgtatgatt
     4381 acacgaaaag taaattccgt gcaggaagct ctgagcggca ttacagataa tatgaccatc
     4441 atgttgggtg gatttggact atgcggtatt ccggaaaaca gcattgccga attagtgaaa
     4501 ttaggtattt ctgatttaac ttgcatttcc aataatgccg gagtggatga ttttgggtta
     4561 ggattactac tccaaaaaaa gcagattaaa aaaatgattt catcttatgt gggagaaaat
     4621 gcagagtttg aacgccaaat gctttccggt gaacttgatg tagaactcac tccacaagga
     4681 acattagctg aaaaatgtag agcggctcaa catggaattc ctgctttttt tactcctgcc
     4741 ggatacggaa ctgaagtagc tgaaggaaaa gaaacccgcg aatttaacgg aaaaatgtat
     4801 gtcatggaaa aagcctataa agctgatttt gccattgtaa aagcgtggaa aggtgatgaa
     4861 gccggaaact taatattcaa aggaacagcc agaaacttca attcttgtat ggccggagca
     4921 gctaaaatca cgattgcaga agtagaagaa ttagtgcccg ccggaactct agaccctaac
     4981 caaatccaca ttccgggaat aatggtcaat cgtattttta aaggggaaaa gtttgaaaaa
     5041 agaattgaac aaagaaccgt tagacaaaaa gcataaatca tggcactaga taaaaatcaa
     5101 attgcaaaac gaatagcaaa agaagtaaaa gacggttact atgtaaattt aggaattggc
     5161 attccaacat tagtagcaaa ttttgtcaga acagacattt ctgttgaatt tcaaagtgaa
     5221 aatggcgtac ttggtatggg tccatttcct tttgagggtg aagaagatgc cgatatcatt
     5281 aatgcaggaa aacaaacaat cacgacttta cccggagctt ctttttttga ttcagcattc
     5341 agttttggga tgatacgaag taaaaaagta gatttaacta ttttaggtgc aatggaagtt
     5401 tctgaaaagg gagacattgc taattggaaa atccccggaa aaatggttaa aggaatgggt
     5461 ggagctatgg atttagtagc ttctgccgaa aacatcatcg tggccatgat gcatgttaat
     5521 aaagctgggg aatccaaagt ccttaaaaat tgtacattac cattgacagg ggtcggttgt
     5581 gttaaaaaaa tagtaactga attagcagta atggacgtaa caccaaacgg ttttaaatta
     5641 cttgaaagag caccgggagt aaccgttgaa gaaattatca aagctacgga agggaaatta
     5701 attgtagaag gtgaaattcc tgagatgatt ttagattaaa tattgcttaa aaaaaattaa
     5761 agctattaaa attcaacctt taatagcttt ttatttgttt aaataggcca acaaaacctc
     5821 attcgcattg ccaaaagttg gatgttgatc ggtgacactc accactcttt ttttgtttaa
     5881 tttatacgtc aaatcaaatt ctgttgtaaa taaaataacc gataaaaacg ggttattaat
     5941 aaaaggaagc aagcgattaa aagcactacc aacagaatga atttcaaaca actcttcttt
     6001 ttgaaatcgg aattttagtt ctaatttcaa ctcattttct tctaaaatag gacgtataaa
     6061 aatatttttt aattccgttt ttccaatggt tttggcaaaa gtaagtttgg caaaagtnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgt tgccgctgtc ctcctgaaaa
     6241 ctcatgcgga tatcggttaa aaaaggattc tcctaaacct actctttcca aaatttcgat
     6301 ggtttttgct tttcgttcgg catcattagc aaataatcca tgcactttca tgggttccat
     6361 aatcgctttc ccaattggaa ttcttggatt taaagaagaa taaggatctt gaaaaatgat
     6421 ttgaatttct tttcgtaatt ttcgaatata ttccttagtt aagtttgtaa tgtcatttcc
     6481 tttataaaaa atttgtcccg atgtagcttt atctaattgc aaaatagcat ttcctaatgt
     6541 tgattttcca caccctgatt cccctactaa gccaactgtt tcgccttcat aaattttaaa
     6601 actaacctca tttacggctt taaaagaatg ttgctttcca aataaaccta ctgaagaaaa
     6661 atagtctttt tcaatatttc ttacttctaa aagtggcgat tttgaataga ttttcaattg
     6721 gtttatattt ctttcctctg cagatactat ttcacttttg aatgattctt tcaaaaaatc
     6781 ttgaatggtg ggaagccttt tcaagcgaaa atccaatgaa ggccgcgagg caataagagc
     6841 tttagtataa ttatgttgcg gattttcgaa tatttctttg gcaattccct gttctacaat
     6901 ttgaccttta tacatcacta aaacccgatt agcgatttca gaaactaaag ataaatcgtg
     6961 tgaaataaag agtatgctca tcttcgtttc tctttgcaac tcttttagca attgtattat
     7021 ttctttttga acggtaacat ccaaagctgt tgtgggttca tctgcaatta aaattttagg
     7081 tttacaagca attgccatag ctatcataac ccgttgcttt tgtccgccgg aaatttggtg
     7141 aggatattta gaaaacagtt cttccggatt tggtaatttt actttttcaa aaagagaaat
     7201 tacttgattt ttaatttctt tttttgaaag tgaagtgtgt atttctaaaa tttctgctac
     7261 ttgcatgcca catttcatcg aaggattcag tgaactcatg ggttcctgaa aaatcattgc
     7321 aatttcattt cctctgattt tttgaaattc ctgatccgac aataaggaaa tatctttgcc
     7381 ttcaaaaacg attgaaccat tggcaatttt tgaaatagaa gaaggcaaca aacccatgat
     7441 tgccaaggaa gaaacagact ttcctgatcc ggattccccg acaattccta aaatttcatt
     7501 ttcatataat tcgtaggtta attgatgaac aaccggaata aattctttct ctcttttaaa
     7561 ggaaatggtc aagttttcta ccgataacag cttttgatta ttcatgtttt aaaattaatt
     7621 tatttttttc attttcatct taaaaatatt ttttaaaata aaaatgagct ataataaaat
     7681 ttagagatga cttaagagta atataaattc ttttaaagga agtaatattt tatatctttg
     7741 aaatgattta aaaataaatt gatgaaaaat atatatttct caggtaaaat tattgttcta
     7801 ataatgattg tttttacgac tccttttttg ttaaaagggc aaactgttga acaaaaaaaa
     7861 gcattattag aacaatatga tattgctaaa attaaaacac tacaagttca gtttgcaaaa
     7921 aaagccaaga cagaaaaaga aaaagctatc cttgctgccc gggctaatca gtgggagatt
     7981 tttaaattta atgcggatgg aagttttgat gagttaattg gtctacatcc agacggaagt
     8041 ccaaaatatt tttccataaa caacgtaaat gcagcaaaat caacacgaac caaccactta
     8101 aattccggtg gaagtttagg cctcaattta aatggtcaaa acatgattgg aggagtttgg
     8161 gatggtggtc cggttagaat atctcatcaa gaatttggag gaagagttca aataggagac
     8221 ggtgaaactg ttttaaactc aaatagtttc catggcacgc atgttaccgg aacaataacg
     8281 gcaaccggcg ttcaagcaaa tgcaaaagga atggctaatt tagcaaccgt aaaaacattt
     8341 gattggagta atgatgaggc agaggtttta gctgaaattc aaaatggttt attgttatca
     8401 aatcattcgt atggtgttcc gatttcaagt gctcctgggc catggtatat aggagcctat
     8461 tctgcagata caagagcatg ggatgaaatt caatacgttt caccttatta tttaatggtt
     8521 gtttctgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg aaatgccggg
     8641 ccggatgcaa aatttggttg gggtcttttg aatgcaaaag cggctgccat tgccatcaca
     8701 aacaatggat taaatacttg gatttctgaa gaaaaaatag cgacaaacga gacctacaca
     8761 atgactgtaa aatctattgc aggagctcct ttaaccgcaa cgatttgttg gacagatgtt
     8821 cctggaattg ttaataacgg aatattaaat agctcaattc ccgctttagt aaatgattta
     8881 gatattagaa ttacacaagg ttccaatacc tatttcccat ggcggttaca atctaatgct
     8941 tctcttttag cagtaagaaa cggggataat tttgtagata ccgttgaaac aattaaaata
     9001 gattcagcta atggtggaga ttataccatc actatttcca gtaaaggaac gttagtcaat
     9061 aatgatcaac ctttcggatt gattatttca gggattgatt cttcttttgg aatagttcct
     9121 cttggatatg atcaagtaaa atgtgccaac gaggaagccg tcttttcatt taatttcaat
     9181 ggtgcatcaa gtgggaatgt aaatttatcg gcaataaata tacctgccgg agctaacgtc
     9241 aattttagtt caaaccccat aaacatgaat ggtattttta caatgactgt ttcaaattta
     9301 tcaaatgtta ttccaggaga atattccatt ggagtagtag gaagtaatgg attggattca
     9361 gaaacccgtt atgtaaattt aaagattttg agcaatcaat ttaattctgt tacaacgacc
     9421 taccctataa atggtcaaac aggtctttca accaccttaa atttaacatg ggaagaagat
     9481 attaatgctg aaaattatat gctagaaatt gctacagatt ttaattttaa ttcaatcatt
     9541 gaatctcaat caactgcaaa cacaaattat accgcaacta acttagcaag tgaaaccgtt
     9601 tattattgga gggttaaacc aagtaatcgt tgtggaactt caacaaatta tagtgtttcc
     9661 agttttcaaa ccggtatttt gaattgtcaa aatcaattta taggaacaga tttttcagac
     9721 gcctctattg gcagtgtagc aaattcattg gcttctgtcc cagtaaccgt ttcgggtggt
     9781 tttgtcattg gagatataaa tgttacttta aatttaacac atacttgggt tcaagatatg
     9841 acaatttatc ttgatggccc ggcttctatt ggaagtccga gaataacttt gtttgaggag
     9901 ccttgcggtg gagaagatga tatagatgcc attttagatg actccggatt agatttggag
     9961 tgtggaataa acccagcacc cgcaatttca ggaattataa aaccactaca actattaagt
    10021 aatttaaaca gtttacctgc tgatggaatt tggaaacttc aagtgattga taattataat
    10081 ggtgatggtg gaagcatcaa ttcattttca ttgaattttt gtagtttatc aactgttaca
    10141 aataatttaa attttgaaaa caatggaatt gtaactacaa cgtcaagtaa taaagttatt
    10201 ttaaacacgg aaataaatgc ctcaacacct tctcaatcag acaataacca aacttataca
    10261 cttacagaaa cacctattct tggactgctt acaaaaaatg gaacagcact tacaataggt
    10321 aatactatta cacaaagtga tataaatgaa ggtcgaataa gctattcaaa ttcacaattt
    10381 gaacccggtc aagattcgtt taaagcagat atacttaaca gttcttcagg ttggttgcca
    10441 aataataccg ttataattac gattgaaaac agtttaagta catcagaaaa tttaatggaa
    10501 ttcgttcgta tttaccctaa tccaactcaa aattatatca caattcagct cacaaatgaa
    10561 atagtgggag aaactaaaat ggatttattt gatgttcagg gaagaaaaat tatctcaaaa
    10621 aatacatttc agaatactga agaattaaat cttagtagcc tatcaggtgg catttatttt
    10681 ctttctattc aaaataaaaa ccacaaaatc attaaaaaaa taattttaaa gaaataaaaa
    10741 aactaaatag aggaaatcaa tttatacatg aataatttcc tcttcaatta ataaatcctc
    10801 tctacgtaat cgtaaaaaaa cttgtgcaac ggctatcaca tccttttcac aataagtgat
    10861 gatacggtca atatcttttt ctacataata aacataggct acttcactac cgtcaatatc
    10921 atctttagga gatggaattt gcaggacttt agtgagtaaa cgcatggaag tataatgttt
    10981 gtaatcacca aatttccaaa gctctagcgt gtctaaatgc ggaacttccc aaggtttttt
    11041 gccaaataaa ttcaatttag ctggaatttc aatttgatta ataatcattc ttctggcgat
    11101 aaaaggaaaa tcaaattcct tggcattgtg gccacataaa atatgctgtg gttgattaaa
    11161 atggttattg agtaaattgc tgaaatcctt caaaatcttt ttctcttctc caaaaaagga
    11221 agtgactcta aaatttcgga catctccttt agttataaaa taacccactg aaatacaaac
    11281 aatttttcca aattcagccc aaataccggc tttgtcataa aattcttcag gagtaaattc
    11341 gtcttttcgt tggtactgcg ttttggtttc aaaaagatac tgcttttctt catcaagcaa
    11401 tttaaagttt tcttcttccg gaacagtttc aatatccaga aataaaatgt tgtcgagttt
    11461 aattttttct atcatggctt attaatttac ttttataaaa gtaaaaaaaa agtaggataa
    11521 aaaattcaat tgttaaactg ttttttgaaa tttaattaaa taaaaaacca tctcttttaa
    11581 aaaagatggc ttaattgaat ttataattta gcagatattc ttattgtgta acaggagcat
    11641 catccaatac tttcccttta atagtaagta tttttggtac ttcattttca tttaaagaaa
    11701 cagtaactgt ttttgaaaaa gcacctgggc ttgcagcatt ataagttgct gtaatggtag
    11761 ctttttcacc gggtttaatt ggtgttttag tataatttgt agccgtacat ccacaagatg
    11821 cttttacatt agtaactaaa actgtttgtt tagttgtatt cacaaaagtg aaatcatgag
    11881 aaactggtgt tccttttttg atgtctccaa aatcaaaaat ctcttctttc cattttacag
    11941 gagttgttgg cttttgtgga gcaggagttg tagtgatagc aggagttact gctgccttct
    12001 tttcttgagc atttgcacta actgaaaaca aaactgttgc agcaaaagca aataaagata
    12061 cttttaaaga tttcatactt attattttta aattattgat taattcgttt gaacttagca
    12121 aatatattca caagaaagcg gatatttgtt aagcacatca taagttttgt taatgagttg
    12181 ttaatcagaa aaaaatatac gcttgtgtta gtagttttgt gtctaaatct aatattaaat
    12241 tgaaatttaa ccgtttaaat agtatcattt ttgccggatt cctatcaatt gtaggggtgt
    12301 tgattatgca attgttaatg ctaaatcggg cctattcttt tgaaaaaaaa gagtttgaag
    12361 ataaaattca ctttgcttta caagatgtgg taaaacggat ttatcgtgac aacaaaactg
    12421 atttagccat tactaatcaa attaaaaaaa ttacggacga ttattacatt gtaaatattg
    12481 acgatgtttt tgaacaccaa gttttagaat attatttaaa atctgaattt caaaaagtaa
    12541 aacttggtat tgattatgaa tatgctattt ataattgtgg aacagatgaa atgatatacg
    12601 gaaattatgt ttccagtgat tcgggtaaac cggagaagtg tgaaaactgt tttcaaaaaa
    12661 atgaaggttt aatctattat ttcggaattc gatttccgga tttacaacat aaatttatta
    12721 cttcattagg tcaatattgg atttacaccg gcatattgtt tttggtttta attatttacg
    12781 tatactctgt ttttctttta ttaaaacaaa aaaagtatac cgaattgcaa actgatttca
    12841 taaataacat gacccatgaa ttcaaaacac cactttcgtc aattttaatt gcttcacaat
    12901 atgcctcttc acaggatgaa attaaaaaca atccaaaatt ggccaaatac atgcaaatta
    12961 ttacgaacca aagccataaa ttaaatcaac atattgaacg gattctaaat gtggcaaaag
    13021 gagatgcaaa atggatagaa ttggacaaaa aacccattaa tattaaagac gctttagaat
    13081 tagttaaaga aaatgctatt ttaaaatcaa ctaaaaaaag tgttgttaaa attgaaatac
    13141 cttctaaaat ttctgttttg gccgacgaat ttcattttaa taatgtttta tataacattg
    13201 ttgataatgc tttgaaatac tgctctgaaa gccctgaaat agtaataaaa tcagttgaaa
    13261 acgatagagg aattactctc catttttcgg ataatggaag cggaattgct ccacaacaca
    13321 ttgattatgt ttttgataaa ttctatcggg tcccgcgtga aaacaaaaaa gatattgaag
    13381 gttttggtat tggtttattt tatgtaaaaa aaataattga tttacatcat tggaaaatag
    13441 ctattcaaaa taatgaaaat caaggcataa cagtttttat tttcatccca aaaaaagatg
    13501 ttgtatgagt aaaaagaaaa ttctttatgt tgaagatgat gaatcattag gttttttaac
    13561 tgctgatagt ttagaaaatc aatatgatat tgcccatttt attaatggga aagaaggttt
    13621 tgatgccttt tgttcaaatg aatatgattt atgtatttta gatgtgatgt taccggatat
    13681 ggatggcttt gaaattgcaa cggccattcg aaaacgaaat ctagaaatcc ccattatttt
    13741 tctttcagca aaaaccatga aagaagaccg catcaaagga ttaaagttgg gtgccgatga
    13801 ttatttggtt aaaccttaca gcatggaaga attgaccttg aaaattgaag tatttttgaa
    13861 tcgaagtcaa aaaacaatct ctaaaacaac aacaacttat ttaattggtc catttgaatt
    13921 tgatccgaca aattattgtg tttccaaagg aaaagaagtg acaaacctca ctgaaagaga
    13981 agccgcctta ttaaaattct ttttagagaa taaaaatact gttttaaaaa gagaaaaaat
    14041 tttgatggaa ctttggggaa ctgatgatta ttttgtgggg agaagtttag atgtttttat
    14101 ttcccgcttg cgaaaaattt ttaaagaaga acccaacatt cggattgaaa atattcc
//
LOCUS       DAOTCW010000057        14084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2572_length_14084_cov_51.219331, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000057 DAOTCW010000000
VERSION     DAOTCW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14084
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2572_length_14084_cov_51.219331"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="P5335_06255"
     CDS             complement(<1..302)
                     /locus_tag="P5335_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HRZ74514.1"
                     /translation="MLKQNLQFKLSQKLSPQQIQLMKLIQLPTQAFEQRLKEEMNENP
                     ALEGGKEEDEFADDFEKEEFDDYEEDEYDRIDAEDINIDEYLSNDETPEYKLQANN"
     gene            complement(347..1795)
                     /gene="asnS"
                     /locus_tag="P5335_06260"
     CDS             complement(347..1795)
                     /gene="asnS"
                     /locus_tag="P5335_06260"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRZ74515.1"
                     /translation="MKKHTKVIELLNATPVQTEVKVKGWVRTFRNNQFIALNDGSTIH
                     NIQCVVDFENTKEELLKRITTGAAISLTGTLTESKGAGQKVEIQVAKLEILGDSDPEK
                     FPMQPKKHSLEFLRENAHLRIRTNAFGAIMRVRSTLSFAVHQYFQERGFFYVNTPIIT
                     GSDAEGAGEMFKVTALPFDKTPRTEEGKVNYKEDFFGKETNLTVSGQLEAETYAMALG
                     QVYTFGPTFRAENSNTSRHLAEFWMIEPEVAFNNLADNMDLAEDFIKYVIKYTLDKHQ
                     DDLKFLEQRLIEEEKNKPQTERSEMNLLDKLNFVLDNNFKRVTYTEAIDILRNSKPNK
                     NKKFQYAIDEWGADLQSEHERFLVEKHFKCPVILFDYPAKIKAFYMRLNDNTEPGKET
                     VRAMDILFPGIGEIVGGSQREERHDVLIEKMKALGIDEEELWWYLDTRKFGTAVHSGF
                     GLGFERLVLFVTGMANIRDVIPFPRTPQNAEF"
     gene            complement(1843..4251)
                     /locus_tag="P5335_06265"
     CDS             complement(1843..4251)
                     /locus_tag="P5335_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRZ74516.1"
                     /translation="MSKWFSVGFWESVARIILRNRITILSIIFLFTVFLALQWKNMRF
                     TYTEANLLPDHHEINLQYNDFLDKFGEEGNLIVIGIKDSTFFTPKNFSAWTQLMETLK
                     KSKEVELIISVDDLKKLNKNETLETFEFIDFINKSKSNDPDYLIARQKELFEELPFYE
                     HLLINKKTGTIRSAIYLDKKIVNTSIRKDFIEKVLIPEVEKFEKTTKIDLKVSGMPYI
                     RTLNSQNIVDEIGIFIGAALLVTSLIFFFFFRSYRATIISMCIVIVGVMWSFGTLGLL
                     GFEITVLTAIIPPLIIVIGIPNCIFLINKYQQEIKNHGNQAKSLQRVISKVGNATLMT
                     NLTTAAGFATFIITDSELLTEFGIVASINIVLLFILCLLVIPIIYSFMPLPKDKHLKH
                     LNKTYTTSFVNWIEKTVKNNRISIYATSITLLVIGIIGIYQMRISGSLIEDMPKKTGF
                     FKDIVFFEKEFDGIMPLEIMIDTKRKKGVMKLSTLKKMDEIQEVIIDIPELSSPMSVV
                     NVVKYSKQAYYNGNPEYYELPTAQEQGFILSYAKNATKNKKENFMKSYVDSTGQYARI
                     TTFMKDIGTDKMEKIEEKLWDKINKVFPPEKYTVSLTGKALVFQKGTNYLVNNLVSSL
                     IFAIFLISLFMAYLFRSFKMIIISLLPNLLPLIMTAGLMGYLGVPIKPSTILVFSIAF
                     GISVDDTIHFLAKYRQELKANNWKIRKSVFNALRETGVSMFYTSIVLFFGFSVFMISS
                     FGGTVALGGLISTTLLFAMFSNLILLPALLLSLEKTIANKEEFIEPTIDILVEEDEET
                     ELKS"
     gene            complement(4369..>6785)
                     /locus_tag="P5335_06270"
     CDS             complement(4369..>6785)
                     /locus_tag="P5335_06270"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRZ74517.1"
                     /translation="KDQTNQKPLPFVTIETNTGIISTSDVTGKFEISSNSTLNSLIIS
                     YIGYETLTLYIKDKKHLSVLLIPKKTNLNSTLPASENDEAVVLIEKVIEKQDFNNPQK
                     KLSNFQFKIYNKLIVTANPDSISGKIDSIYYGKKNDIKIDSSNHKFKKLVTKQHLFLT
                     EKVSLFQQENQKVKETVLGTKMSGFKNSLYEIIGFHLQSFSLYDEKYEFFETKYVSPI
                     SKTGLKDYDYKFLSSVKIAQRDCYVLYFKNKKKINQKGLEGVLYIDAENFGLAKAIIR
                     IRGILDITSIHEFEFIKQENLWFPKNNAFTVVKGQNDDPLKILGGTIFFEGDYEEMGL
                     KKKKGPTDFSYIHSESYFFDREFNVPVDIKRSSVVIEINKDAVNKTTDFWNLYRTDSL
                     DFRSKNTYKSLDSLAYRKGVDSKLFFGRKIINGYAPLGFFDFDLRYLISYNNYEGFRF
                     GIGGKTNEKLSVKYRLEGYAAYGLKDEAMKYKIGGAKRFDEFSNTWMGVNYTDDVQEI
                     GSTKFTIDKRVFKIYDPRPINVNTFYHHQTWNGFIESKLLPKSESIWQFSQAKITPLF
                     NYLFYNDGEEYSVFNMTTASLSLQWNPFSDFMQTPTGKIEIEKRFPVFTLQVTQTIPN
                     SLGNDFDYTKIDFRFKYQKKFLNGQKSTFLLQSGLALGDVPITHLYSSSPNNLDRSTI
                     LKRITVAGENSFETMFYNEFFSSRYFMLHLKHGFKRIEILPKVKPSLALVTRMAWGNM
                     DNPEQHLGIEYKTLNEGYFESGIELNHILYGFGLGGYYRYGPNQLSKFEDNIAIKITF
                     TFDLGF"
     assembly_gap    6786..6885
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7084..7647)
                     /gene="frr"
                     /locus_tag="P5335_06275"
     CDS             complement(7084..7647)
                     /gene="frr"
                     /locus_tag="P5335_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRZ74518.1"
                     /translation="MTEEIEFIIDTAKEHMNGSIAHLEKEFLNIRAGKATPQMLGGVF
                     VDYYGSQTPLSQVANINVPDARTITVTPWEKNMLHPIEKAIQVANLGFNPMNNGDNII
                     ISVPALTEERRRDLVKQAKSEAEDAKIGIRNARKDANTDIKKEEKNGTSEDMCKDAEE
                     TIQKLTDTYIKKIEDLLAHKEAEIMKV"
     gene            complement(7724..8431)
                     /gene="pyrH"
                     /locus_tag="P5335_06280"
     CDS             complement(7724..8431)
                     /gene="pyrH"
                     /locus_tag="P5335_06280"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRZ74519.1"
                     /translation="MKYKRILLKLSGEALMGDRQYGIDPKRLAEYAQEIKQIHSQGVE
                     IAIVIGGGNIFRGVAGASNGMDRVQGDYMGMLATVINGMALQGALEEAGMLTRLQTAL
                     KIEAIAEPYIKRRAVRHLEKGRIVIFGAGTGNPYFTTDTAAVLRGVEVGADVILKGTR
                     VDGVYNADPEKNKDAVKFDYISFEEVLSKGLNVMDTTAFTLSQENKLPIIVFDMNKPG
                     NLLKVCNGETIGTTVTI"
     gene            8647..9261
                     /locus_tag="P5335_06285"
     CDS             8647..9261
                     /locus_tag="P5335_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRZ74520.1"
                     /translation="MKEIIKKSLEKSFSYTEYRNHVSNKLAQGEVTGNEQSEHLLKYS
                     ELNVVRMNRLDKTLTVHEDVSEKISSIKSKLIWLVLSEGWCGDAAQLLPVINKKAELS
                     ENIKLKVLLRDENEELMNQFLTNGGKAIPKLIIIEEETLEILDHWGPRPEGASKLISD
                     YKATHGIVDEIAKTELQKWYLQDKGFSTQKEIIEKLEEIEKQLV"
     gene            complement(9253..9870)
                     /locus_tag="P5335_06290"
     CDS             complement(9253..9870)
                     /locus_tag="P5335_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HRZ74521.1"
                     /translation="MAKISWLLLLIPIFLVAQKSKLKTGDLLFQKMNCGELCEAIHAV
                     TEGYNGNDFSHLGLVYIQNDSVFVFEAAGNAVRKVTLEQFSKNTQNTMMIGRVKSKYK
                     KVIPLVISFSNQQIGIPYDDDYLYDNGKYYCSELIYDAFLGAYGKPFFELKPMTFKKP
                     NSNDFFPAWVEYYKNIGIEIPEGKPGCNPGGISTSDKIKIIGKLN"
     gene            complement(9857..10564)
                     /gene="truB"
                     /locus_tag="P5335_06295"
     CDS             complement(9857..10564)
                     /gene="truB"
                     /locus_tag="P5335_06295"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRZ74522.1"
                     /translation="MKSAQDFLDGQILLIDKPLKWSSFQAVNKLKYTLKRELGLPKKF
                     KIGHAGTLDPLASGLLIICAGKFTKLITEIQSQSKEYTGTFMLGATTPSYDLETEINQ
                     TFPINHIDENLIQNTLVQFIGEIDQKPPVFSAIKKDGIRMYEHARAGAEIEMESRKTT
                     IHEFEITRIELPEIDFRVVCSKGTYIRSLANDYGKALNSGAHLAVLRRTKIGDFDVLN
                     AISPEEFEKQMKNHGEN"
     gene            complement(10571..11377)
                     /locus_tag="P5335_06300"
     CDS             complement(10571..11377)
                     /locus_tag="P5335_06300"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRZ74523.1"
                     /translation="MDLLQAVIIAVVEGLTEYLPVSSTAHMIFTSSYFGIQEDDFVKL
                     FQVSIQFGAILAVVFLYWRKFFDFSKFNFYIKLAAAVIPALILGKLFDDKIDTVLGNP
                     IPIAIVLILGGIILLFVDNRFQNPTVVSEEEITIKKAVMIGFWQCLAMMPGTSRSAAS
                     IIGGMQQGLTRATAAEFSFFLAVPTMLAVTVYSLFLKTYEVTQLKGYELLLQGDNIKF
                     FLIGNVIAFIVAVLAIKLFIEVIKKYGFKPWGYYRIVAGIILLLYFSLNK"
     gene            complement(11379..11621)
                     /locus_tag="P5335_06305"
     CDS             complement(11379..11621)
                     /locus_tag="P5335_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HRZ74524.1"
                     /translation="MSSKNEQKQEFLFEKINYKILLIGIGIIALGFILMSGGGSDDPK
                     VFSEDIYNFRRIRLAPTVVLAGFGVVIYAILKNPKK"
     gene            complement(11644..12519)
                     /locus_tag="P5335_06310"
     CDS             complement(11644..12519)
                     /locus_tag="P5335_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRZ74525.1"
                     /translation="MASSFEKYQKRRLISSYFSVVLSVFLVLFLLGILGLFVINSKKI
                     SNDFKEEIPMSVYFKDNANDSVLKSFGTMLKESAFVKTDTFVSKEMAASKHKDIIGED
                     FMQFLGFNPLQNSYDINLKADFVQADSIKTIEQKFKENKFISDIVYDKHLVDLVNDNV
                     KKITFWILVITGFLALIAVLLINSSMRLSIYSNRFIIKTMQMVGATKSFIRKPFIMRS
                     IKLGVVGALLAIVAIIGVLFYIDANFPKLGILDDQLMIGAVLLSVLVLGVLISWFSTF
                     FATQKFLNLRTDDLY"
     gene            12670..>14084
                     /locus_tag="P5335_06315"
     CDS             12670..>14084
                     /locus_tag="P5335_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRZ74526.1"
                     /translation="MKYFHEQVEEKWQKYWKDNYTFKAQNNSDKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIVARYKRHKGFNVLHPQGYDSFGLPAEQYAIQTGQHPEKTTKE
                     NIARYREQLDKIGFSFDWSREVRTSNPDYYKWTQWIFIQLFESYYCKVADKAYPISEL
                     IKVFEAEGNTNVDLVCDDNVALFSASEWNTFSSEKKQTILLKYRLTYLAETEVNWCPA
                     LGTVLANDEIVNGVSERGGHPVIRKKMTQWSMRISAYAERLLQGLETIDWSESIKESQ
                     RNWIGKSVGATVKFKVSGSKFQIEVFTTRPDTIFGVTFMTLAPEHELVSQITTPEQKA
                     AVEAYVEATAKRSERERMADVKTISGVFTGAFAEHPFTKEPIPVWIGDYVLAGYGTGA
                     VMAVPCGDERDYAFANFFKGSKGMPEIKNIFNQDISEAAFAEKVGFELVNSDFLNGLG
                     YKEGTKKIIEALEAIGQGKGKT"
BASE COUNT         4544 a         2336 c         2247 g         4857 t
ORIGIN      
        1 taattattgg cttgtaattt atattccggg gtttcatcat tactcaaata ttcatcaata
       61 ttgatgtctt cagcatcaat acgatcatat tcgtcttctt cataatcgtc gaattcttct
      121 ttttcaaaat catccgcaaa ttcatcttcc tcttttcctc cctctaaggc aggattttca
      181 ttcatttcct ctttcagacg ttgttcaaaa gcctgcgtag gcaattgaat taacttcatc
      241 aactgaattt gttgaggaga taatttttga gataatttga actgtagatt ttgttttaac
      301 atttttattt ttttaaactt agttacctag caacgcagta acttttttaa aattctgcat
      361 tctgaggagt tctaggaaat ggtatcacat cacgaatatt agccattccg gttacaaata
      421 gcactaaacg ctcaaagcct agcccaaaac cggagtgaac ggctgtgcca aattttcgtg
      481 tatctaaata ccaccataat tcttcttcgt caattcctaa tgctttcatt ttttcaatca
      541 agacatcgtg acgttcttct ctttgtgatc caccaacaat ttcaccaatg cccgggaata
      601 agatgtccat agctcgaacg gtttcttttc ctggttccgt attgtcattt aaacgcatat
      661 aaaacgcttt aattttagcc ggataatcaa ataaaatcac aggacattta aagtgttttt
      721 caactaaaaa gcgttcatgt tcactctgta aatcggcacc ccattcgtca atagcatact
      781 gaaatttttt attcttattt ggttttgaat ttcttaatat gtcaatggct tcagtataag
      841 taacgcgttt gaaattgtta tcaaggacaa aatttaattt atccaataag ttcatttcgc
      901 tgcgttcagt ttgtggttta tttttttctt cttctatcaa gcgttgctct aagaatttca
      961 agtcatcttg gtgtttatcc agcgtatatt taatcacata tttgataaaa tcttccgcta
     1021 aatccatatt atcagccaaa ttattaaaag ctacttccgg ttcaatcatc caaaattcag
     1081 ctaagtgacg agaagtattg gagttttcag ccctaaatgt tggtccgaaa gtataaacct
     1141 gacctaaagc catcgcatag gtttcagctt ctaattgtcc ggaaacggta agattggttt
     1201 cttttccaaa aaaatcttcc ttataattta ctttcccctc ttcagttctt ggtgttttat
     1261 cgaaaggaag agcggtaact ttaaacattt ctcccgcacc ttcagcatct gaaccggtaa
     1321 taattggtgt gttcacatag aaaaaacctc tttcctgaaa atattgatgt acggcaaaag
     1381 aaagcgtaga acgtactctc atgatagctc caaaggcatt cgttctaata cgtaaatgtg
     1441 cattttccct taaaaattcc aaagaatgtt ttttaggttg cattggaaat ttctctggat
     1501 cagaatcgcc taaaatttcc aatttagcga cctgaatttc aactttttga cctgctccct
     1561 tactttcagt caatgttccg gtcaatgaaa ttgctgctcc ggtggttatt ctttttaata
     1621 gttcttcttt tgtgttttca aaatcgacaa cacattggat attatgaatg gttgaaccat
     1681 catttaaggc aataaattga ttgtttctaa atgttcgaac ccaacctttt acttttactt
     1741 cggtttgaac aggagtggca tttaataatt caataacttt cgtgtgcttc ttcattttaa
     1801 atttgttttc aatgcaaata tactaacggg ttactatttt ttttatgatt ttaattcggt
     1861 ttcttcatct tcttcaacta aaatatctat agtaggttct ataaactctt ctttgttggc
     1921 aattgttttt tctaatgaaa gcagtaaggc aggtagtaaa attagattag aaaacatcgc
     1981 gaaaagtagc gttgtcgaaa ttaatcctcc taatgcaact gttcctccaa aactggaaat
     2041 cataaaaacg gaaaaaccaa agaataatac gattgatgta taaaacatgc ttacgccggt
     2101 ttcccgcaaa gcattaaata ccgactttct gattttccaa ttgtttgcct ttaattcctg
     2161 acgatatttt gctaaaaagt ggatagtgtc atcaacagag attccgaagg cgatactaaa
     2221 aactaatatt gttgaaggct ttataggaac acctaaataa cccattaaac ctgccgtcat
     2281 tattaaaggc aacaagttcg gtaaaagaga aattataatc attttaaatg acctgaataa
     2341 ataggccatg aacaatgaaa tcagaaaaat agcaaatatc aatgatgaaa ctaaattatt
     2401 aactaaataa ttagttcctt tttgaaacac caatgctttc cctgttaaac ttactgtgta
     2461 tttttcagga ggaaatactt tatttatttt gtcccataat ttttcctcta ttttttccat
     2521 tttatccgtg ccgatgtctt tcatgaaagt ggtgattctg gcatattgac ctgtagaatc
     2581 cacatagctt ttcatgaaat tttctttctt gtttttagtt gcatttttgg catacgataa
     2641 aataaaacct tgttcttgtg cggtaggcaa ttcataatat tccggattgc cattgtaata
     2701 ggcttgtttg gaatatttta caacatttac aaccgacatt ggacttgaaa gttcaggaat
     2761 atcaattata acctcttgaa tttcatccat cttttttaaa gtggataatt tcatgacccc
     2821 ttttttgcgt ttcgtgtcaa tcatgatttc taaaggcatg atgccatcga actctttttc
     2881 aaagaagaca atatctttaa agaatccggt ttttttaggc atatcttcaa ttaaacttcc
     2941 agaaatcctc atttgataaa taccaataat accaatgaca agcaaagtaa tagaggtagc
     3001 ataaatggaa attcgattat ttttaactgt tttttcaatc caatttacaa atgaagttgt
     3061 atacgtttta tttaaatgtt tgaggtgctt atccttaggc aagggcatga agctgtaaat
     3121 aataggaatg accaacaaac ataaaataaa caataaaacg atattgatag aagctacaat
     3181 accaaattca gtaagtaatt cactatcggt aatgataaaa gtggcaaatc cggcagccgt
     3241 agttaaattt gtcatcaagg ttgcattgcc cacttttgaa attacccttt gcaaagattt
     3301 tgcttgattt ccgtgatttt taatttcctg ctggtattta ttaatcaaga aaatgcagtt
     3361 tggaattcca atgacaataa tcagaggggg aattattgcc gttaaaacag taatctcaaa
     3421 tcctaaaagg cctaaggttc caaaggacca cattacacca acaattacaa tacacatgga
     3481 aataattgtg gctcgataac ttctaaagaa aaagaaaaaa ataagtgaag tgactaataa
     3541 agcagctcca ataaatattc cgatttcatc tactatgttt tgagaattta acgttcggat
     3601 atagggcatc cccgaaactt ttaaatcgat tttcgttgtc ttttcaaact tttcaacttc
     3661 cggaatgagc actttttcaa taaaatcttt cctaatactg gtgtttacaa tttttttatc
     3721 taaataaatt gctgaccgaa tagtgccggt ttttttatta attaataaat gttcataaaa
     3781 gggtaattct tcaaaaagtt ctttttgacg agcaattaga taatccgggt catttgattt
     3841 tgatttattt ataaaatcaa taaattcgaa cgtttcaagc gtttcgtttt tgtttaattt
     3901 ttttaaatca tcaacagaaa taataagttc tacttctttt gattttttta atgtttccat
     3961 taattgagtc caagcagaaa agttctttgg ggtaaaaaaa gtcgaatctt ttataccgat
     4021 gacaatcaaa ttaccttctt cgccaaattt gtctagaaag tcattatact gaaggtttat
     4081 ttcatgatga tcaggaagta ggtttgcttc agtgtaagtg aatcgcatat ttttccattg
     4141 cagtgctaaa aacaccgtga atagaaaaat gatggaaagt attgttattc tatttctaag
     4201 gatgattcgg gcaactgact cccaaaaacc cacgctaaac catttggaca taggattatt
     4261 ttatcggatg caaaggtaat gaaatcaata ctaagttaag ttttttaact aaaattaatc
     4321 tttcttaaaa atttgatgtt tgaaaaaaac caagcactat tatccttttt aaaatcctaa
     4381 atcaaaagta aaagttattt ttatagctat gttgtcttca aatttactca attgatttgg
     4441 cccgtagcga taataaccgc ctaatccaaa tccgtacaat atatgattta attctatgcc
     4501 agattcaaaa tatccctcat tcaaagtttt gtactcaatg cctaagtgct gttcagggtt
     4561 gtccatattt ccccatgcca tacgggttac taaagccaaa gaaggtttca cttttgggag
     4621 aatttcgatt cttttaaaac catgttttaa atgtaacatg aaatatcggc tagaaaaaaa
     4681 ttcattataa aacatggttt caaaactatt ttcaccggca acagtgattc gttttaaaat
     4741 tgtacttcta tcaagattat ttggagaaga gctataaaga tgagtaatcg gcacatcgcc
     4801 aagtgctaaa ccggattgta ataaaaaagt agatttttga ccgtttaaga atttcttttg
     4861 gtatttaaag cggaaatcaa tttttgtata atcgaaatca tttcctaatg aatttggaat
     4921 agtttgggtt acttgaaggg taaaaacagg aaatcttttt tcaatttcta tttttccggt
     4981 tggggtttgc ataaaatcac taaatggatt ccattgtaat gaaagtgaag cagttgtcat
     5041 gttgaaaacg gaatattctt ccccgtcatt ataaaataga taattaaata acggagtaat
     5101 tttagcttgt gaaaattgcc aaatgctttc tgattttggc aataatttgc tttctataaa
     5161 tccattccag gtttgatgat ggtagaaggt gttcacgttg atgggacgag gatcataaat
     5221 tttgaaaact cttttatcaa tcgtaaattt tgtactacca atctcttgta catcatcggt
     5281 ataatttacg cccatccaag tgtttgagaa ctcatcaaaa cgtttggctc caccaatttt
     5341 atatttcatg gcttcgtctt ttaagccata agccgcatac ccctccaatc tatattttac
     5401 agaaagtttc tcgttggttt ttccgccaat accaaaacga aaaccctcat aattattata
     5461 actgataagg taccgtagat caaaatcaaa aaaaccaagt ggagcatagc cattgattat
     5521 ttttctgcca aaaaataatt ttgaatcaac gccttttcta taagccaaac tgtctaatga
     5581 tttataggtg tttttacttc taaaatctaa actgtccgtg cgatataaat tccaaaaatc
     5641 agtcgtttta tttacggcat ctttattaat ttcaatgact acagaagaac gtttgatgtc
     5701 taccggaaca ttaaattccc gatcaaaaaa ataggattcg gaatgaatat atgaaaaatc
     5761 ggttggccct tttttctttt tcaatcccat ttcttcataa tcaccttcaa aaaaaatagt
     5821 tccgcctaaa atttttaaag gatcatcatt ttgacctttt acgacggtaa aggcattgtt
     5881 ttttggaaac catagattct cctgctttat aaattcaaat tcatgaatgg aggtgatgtc
     5941 taatattcct ctaattctta taattgcttt tgccagacca aaattttctg catcaatata
     6001 gagtacgcct tcaagccctt tttgatttat tttctttttg tttttaaaat aaagaacata
     6061 gcaatctcgt tgagctattt ttacagagga taaaaattta taatcataat cttttaatcc
     6121 ggtttttgaa attggactga catattttgt ttcaaaaaac tcgtattttt catcgtaaag
     6181 cgaaaaagat tgtaaatgaa aaccaattat ttcatatagt gaatttttga aacctgacat
     6241 tttagtgcct aaaactgttt ctttcacttt ttgattttct tgttgaaata atgaaacttt
     6301 ttcagttaaa aacaaatgtt gtttggtaac caattttttg aatttatgat tagaagaatc
     6361 aattttaatg tcattctttt ttccataata aatactgtca atttttccac tgattgaatc
     6421 gggattggcc gtaactatta atttattgta aattttaaat tgaaaattag ataatttttt
     6481 ttgcggatta ttgaagtctt gtttttctat taccttttct ataagaacga cggcttcgtc
     6541 attttcagag gctggtaatg ttgaatttaa gtttgttttt ttgggaatta ataaaacact
     6601 taagtgcttt ttatctttaa tatagagtgt tagtgtttca taaccaatat aagaaattat
     6661 caaggaattt agtgttgaat tactggaaat ttcaaatttt ccggtaacat ctgaagtaga
     6721 aataattcca gtgtttgttt caatcgttac aaaaggaagg ggtttttgat ttgtttggtc
     6781 ttttannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtgtt tgtttcaatc
     6901 gttacaaaag gaaggggttt ttgatttgtt tggtctttta taattccggt tattcgaaat
     6961 tgtgcctgaa cacaaaccga acaaaagaga aaaataaaaa gtaaatgttt catagtatga
     7021 aaaaaccaga cacaaaagta ctttataaaa tcaaaaaatc cactctttca agtggattta
     7081 gatttaaacc ttcatgattt ctgcttcttt gtgagcgagt aaatcttcta ttttcttgat
     7141 gtaagtgtca gttaattttt gaattgtttc ttccgcatct ttacacatat cttcagaagt
     7201 tccgtttttt tcttctttct taatatcagt attggcatct tttcgagcat ttctgattcc
     7261 gattttagca tcttcagctt ctgatttggc ttgttttact aaatcacgac gacgttcctc
     7321 ggttaaggcc ggaacagaga tgataatatt atcgccatta ttcattgggt tgaatcctaa
     7381 gttagccact tgaattgctt tttcaatcgg atgtagcata tttttttccc aaggtgtaac
     7441 cgtaatggtt cgggcatcag gcacattaat atttgctact tgtgataaag gcgtttgaga
     7501 accataataa tctacaaaaa caccacctaa catttgtgga gttgcttttc cggctctgat
     7561 gtttaaaaat tctttttcta aatgagcaat agaaccattc atgtgttctt ttgccgtatc
     7621 aataataaat tctatttctt ccgtcatttt gataaatttt ttagatttat ataattatga
     7681 taatttgaaa tgattattct acctttttat aaaattgaac aatctatatt gtgacagttg
     7741 ttccaatagt ttcaccatta catactttca ataaatttcc cggtttattc atgtcaaaca
     7801 caataatagg taatttgttt tcttgactta aggtgaaagc tgttgtatcc attacattta
     7861 gtcctttgct taatacttct tcaaacgaaa tataatcaaa tttcacagca tctttatttt
     7921 tttcagggtc ggcattatat acgccatcaa ctctggttcc ttttaaaatt acatcagcac
     7981 caacttctac tcctctcaat accgcagcgg tatctgttgt gaaataagga tttcctgtac
     8041 cggcaccaaa aataacaatt cttccttttt ctaaatgacg aacggctctt cttttgatat
     8101 aaggttccgc tatagcttca atttttaaag cggtttgtaa tcgggtgagc attccggctt
     8161 cttccaatgc tccttgtaaa gccattccgt taataacggt ggcaagcata cccatgtagt
     8221 ctccttgcac tctgtccatg ccattgctgg ctccggcaac acctctaaaa atatttcctc
     8281 caccaataac gatagcaatt tctactcctt gactatgaat ttgtttgatt tcctgagcat
     8341 attctgccaa acgttttggg tcaataccat attgacggtc gcccattaat gcttcgccac
     8401 ttaatttgag taatattctt ttgtacttca tgagttgtgc ttgattttgt ggtgcaaata
     8461 taaagaataa atgattttat ttttgaaaat tgtatatggg tttctgacta tgtttttaat
     8521 ttctgactat ttgttggttt tcttctgttt ttttattgaa tttagtttct aattttgcga
     8581 agtgttataa atgttacaat ttaagattat aatgtagcct acttttgtgt cctaaaacct
     8641 aagtttatga aagagattat taaaaaaagt ttagagaaaa gtttttctta tacagaatat
     8701 agaaatcatg tttccaataa gcttgctcag ggagaagtta ccggaaatga acaatctgaa
     8761 catttattga aatacagtga attaaatgta gttcggatga atcgtttgga taaaacatta
     8821 acagtacatg aagacgtttc agaaaaaata tcttctataa aatcaaaatt aatttggtta
     8881 gttttatccg aaggatggtg tggtgatgcc gctcagttgt tacctgtaat caataaaaaa
     8941 gctgagcttt cagaaaatat aaagttgaaa gttttattgc gtgatgaaaa tgaggaatta
     9001 atgaatcaat ttcttaccaa tggaggaaaa gctattccta aattaatcat tatagaagaa
     9061 gaaactttag aaattttaga tcactggggg ccaagacctg aaggagcttc aaaattaata
     9121 agtgattata aagcgacaca tggaattgtg gacgaaattg caaaaaccga attgcaaaaa
     9181 tggtatttac aagataaagg atttagtacg caaaaagaga taattgaaaa attggaagaa
     9241 atagaaaagc aattagttta attttccaat tatttttatt ttatcagaag tggaaatacc
     9301 accgggattg caaccaggtt ttccttccgg aatttctatt ccaatatttt tatagtattc
     9361 aacccatgcc ggaaaaaaat catttgaatt aggtttttta aaagtcatgg gttttaattc
     9421 aaaaaaaggt ttaccatagg ctcctaaaaa agcatcataa atgagttcag aacaatagta
     9481 ttttccgttg tcatataaat aatcatcatc atagggaata ccaatttgtt gatttgaaaa
     9541 cgaaataact agtggaatta cttttttata tttagatttt actcttccaa tcatcatcgt
     9601 gttttgcgtg ttttttgaaa attgttctaa tgtaactttt cgaacggcat ttccggccgc
     9661 ttcaaataca aaaacagaat cattttgaat gtatactaaa cctaaatgac taaaatcatt
     9721 cccgttatag ccttctgtaa cggcgtgaat ggcttcgcat aattcaccac aattcatttt
     9781 ttgaaaaaga aggtcacctg tttttaattt acttttttga gcaactaaaa aaatcggaat
     9841 aagtaataat aaccaactaa ttttcgccat gatttttcat ttgtttttcg aattcttccg
     9901 gtgaaattgc atttaaaaca tcaaaatccc caattttcgt tctgcgtaaa acagctaaat
     9961 gagcacctga atttaaagcc tttccataat catttgctaa cgagcgaata taagttcctt
    10021 tgctgcaaac tactcgaaag tcaatttccg gcaattctat tcgggtaatt tcaaattcgt
    10081 gaatagttgt ttttcgactt tccatttcta tttcggctcc cgcacgagca tgctcataca
    10141 ttcggatgcc gtcttttttg atagcagaaa aaacaggagg tttctgatct atttcgccaa
    10201 taaattgaac taaagtgttt tgaattaaat tttcatcaat atgattaatt ggaaaggtct
    10261 gattgatttc tgtttccaaa tcataggatg gtgttgtggc tccaagcatg aaagttccgg
    10321 tatattcttt agattggctt tgaatttcgg tgattagttt ggtgaatttt ccggcgcaaa
    10381 taatcaacaa cccggaggct agtggatcta atgttccggc atgaccgatt ttgaattttt
    10441 ttggaagtcc taactcgcgt ttcaacgtat atttcaattt gttcaccgcc tgaaacgagg
    10501 accattttag tggtttatcg attaagagaa tttgtccgtc aagaaaatct tgtgcacttt
    10561 tcaaaatctt ttatttgttg agggaaaaat aaagtaataa aataattcct gcgacaatac
    10621 ggtaatatcc ccaaggttta aaaccgtatt ttttgatcac ttcaataaaa agtttgattg
    10681 ctaatacggc tacaataaat gcaatcacat ttccaattaa gaaaaattta atgttgtcac
    10741 cttgcaacaa aagttcataa cctttcaatt gagtaacttc ataggttttt aaaaacaaag
    10801 aataaacggt taccgctaac atagtgggaa ctgccaaaaa gaaagagaat tcggcagctg
    10861 tagctcttgt caatccttgt tgcattccgc caatgattga agcagcactt ctactggttc
    10921 cgggcatcat ggctaaacat tgccaaaagc caatcatcac cgcttttttt atagtaattt
    10981 cttcttcact tacaactgtt ggattttgaa aacgattgtc aacaaacaac aaaataatac
    11041 cacccaatat caaaactatt gcaattggaa taggatttcc taaaacagta tcgattttat
    11101 cgtcaaataa ttttcctaaa attagtgccg gaattacagc cgccgctaat ttgatgtaaa
    11161 agttaaattt ggaaaagtca aaaaactttc tccaatataa aaagactact gctaaaatag
    11221 ctccaaattg aatggaaacc tgaaacaatt ttacaaaatc atcttcttga ataccaaaat
    11281 aagaacttgt aaaaatcata tgggctgtgg aagaaacagg cagatattct gtcaaccctt
    11341 caacaacggc aatgattaca gcttgtaata aatccatttt atttctttgg gtttttcaaa
    11401 atagcataaa taacaacccc aaatccggct aaaacaactg ttggagccaa tcgaattctg
    11461 cggaaattat aaatgtcttc gctaaaaact tttgggtcat cgctaccgcc accactcatt
    11521 aaaataaagc ctaaagcaat aattccaatt ccaattaata gaattttata gtttattttt
    11581 tcaaacagaa attcctgttt ttgttcgttt ttactactca ttttgtttgt gtaaaatagg
    11641 ttgttaatac aaatcgtctg ttcttagatt caagaatttt tgtgttgcaa agaaggtgct
    11701 gaaccaggaa attaaaacac ctaagactaa aacactcagt aaaacagctc caatcatcag
    11761 ttggtcatct aaaattccca attttggaaa attagcgtcg atgtaaaata aaacaccaat
    11821 gatagcaaca atcgctaata aagctccaac tactcccagt ttaatgcttc gcataataaa
    11881 aggtttgcgt ataaacgatt ttgttgctcc aaccatttgc attgttttga tgatgaaacg
    11941 gttggaataa atggataatc gcatggaact gttaattagt aaaacagcaa ttaaagctaa
    12001 aaatccggtg attactaaaa tccaaaaggt gatttttttg acattatcgt ttaccaaatc
    12061 aactaaatgt ttgtcgtata caatatctga aataaattta ttttctttaa atttttgttc
    12121 aatggtttta atgctgtccg cttgaacaaa gtcagctttc aaattaatgt cgtatgaatt
    12181 ttgcaatgga ttaaagccta aaaactgcat aaaatcctca ccaataatgt ctttgtgttt
    12241 agaagcggcc atttcttttg aaacaaaggt atcggttttt acaaatgctg attctttcag
    12301 cattgttcca aaagatttta gcacactatc gttggcatta tctttaaaat agacagacat
    12361 tggaatttct tccttaaagt cattagaaat tttcttagaa tttatgacaa ataatcctaa
    12421 aatgccaagt aaaaaaagca ctaaaaaaac acttagaact actgaaaagt aggaagaaat
    12481 gagtcgtctt ttttgatatt tttcaaatga agatgccata gaaaagattt taagcggtaa
    12541 aattaaaaaa gaaattggtt aaaccacagt ttataacgct caaagttttg agtttagtac
    12601 aataaacgta aatttgccct tttaaaaacc ttagcaactc agaaccttag ttcctcagta
    12661 ccttagcata tgaaatattt ccacgaacaa gttgaagaaa aatggcagaa atattggaaa
    12721 gataattata ctttcaaagc acaaaataat tctgacaaac caaaatatta tgtactcgat
    12781 atgtttcctt atccgtccgg tgccggatta catgtgggac atccattggg ttatattgct
    12841 tccgatattg tggctcgtta taaaaggcac aaaggtttta atgttttaca tccgcaaggg
    12901 tatgattcat tcggacttcc ggcggaacaa tatgccattc aaacagggca acatcctgaa
    12961 aaaacaacta aagaaaatat tgcacggtac cgtgagcaat tagataaaat tggattttca
    13021 ttcgattgga gtagagaagt tcgtacttca aatccggatt attacaagtg gacacagtgg
    13081 atttttatac aattgttcga atcctattat tgtaaagtag ccgataaagc ctatccgata
    13141 tcagaattaa ttaaagtttt tgaagctgaa ggaaatacta atgttgactt ggtatgtgat
    13201 gacaatgtag cacttttttc tgcttcagag tggaacactt tctcatcaga aaaaaagcaa
    13261 acaattttat taaaatatcg attaacctat ttagcagaaa cagaagtgaa ttggtgtcct
    13321 gctttaggaa ctgttttagc caatgacgaa attgtgaacg gggtttctga acgtggcggt
    13381 catcccgtga ttcgtaaaaa gatgacccaa tggtcgatgc gaatttctgc gtatgccgaa
    13441 cgtttattgc agggattgga aacaatcgat tggagcgaaa gtatcaaaga gagtcagcgg
    13501 aattggattg ggaagagtgt gggagcgacg gttaagttta aggtttctgg ttcgaagttt
    13561 cagattgaag tttttacaac tcgccccgat accatttttg gtgtaacctt catgaccctc
    13621 gcacccgagc acgaattagt ttctcaaatc acaactccgg aacaaaaagc tgctgtcgaa
    13681 gcttatgttg aagctacagc taaacgttcc gaaagagagc gaatggctga tgtaaaaacc
    13741 atttccggag tatttaccgg tgcttttgcc gaacatccgt ttacgaaaga accaattccg
    13801 gtttggattg gcgattatgt attagccggt tatggaactg gtgctgttat ggcggttcct
    13861 tgtggtgatg aaagagatta tgcgtttgcc aattttttta aaggaagtaa gggaatgcct
    13921 gaaatcaaaa atattttcaa tcaagatatt tctgaagcag cttttgctga aaaagtaggt
    13981 tttgaattag taaactctga ttttttaaat ggtttaggct ataaagaagg aaccaagaaa
    14041 attattgagg ctttggaagc gattggtcaa ggaaaaggga aaac
//
LOCUS       DAOTCW010000058        13544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2735_length_13544_cov_40.506561, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000058 DAOTCW010000000
VERSION     DAOTCW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13544
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2735_length_13544_cov_40.506561"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..599)
                     /locus_tag="P5335_06320"
     CDS             complement(81..599)
                     /locus_tag="P5335_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRZ74527.1"
                     /translation="MKSSSLQSNEYANFYKPYIEALGEVVLMNELELSLSFFIDFLKT
                     IPSEKHEFRYAEGKWTIKDIVQHLIDAERIFAYRALRIARKDKTPLPGFEENEYVETA
                     FANKRVMNDLIEEFITVREATISLFKTFTEEQLLQMGTASEKPVSVRAIGFITLGHQK
                     HHEKIIKERYLK"
     gene            complement(651..3224)
                     /locus_tag="P5335_06325"
     CDS             complement(651..3224)
                     /locus_tag="P5335_06325"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRZ74528.1"
                     /translation="MYTLPKIERFHQNVLSKYQIYNSVFITLPFDAIDNTGVLLPLFS
                     EVCENGFKNLQSPEEIFNLFASKYLDHPSEDRKIELMFRFIQYVERQIVLFDAIEDAA
                     FPIVNNMEGRGSLRDMKEKADQRSKGIELKEFLENFKVRTVLTAHPTQFYPGSVLGII
                     TDLTEAVRVNDLIQIKKLLAQLGKTPFIKKEKPTPFDEAISLIWYLENVFYEAAGGMI
                     QFLQKNIFDGQEIHNSILNLGFWPGGDRDGNPFVTTEITLKVAERLRTSILKCYYNDI
                     RKLKRKLTFKEVDTIIAEIEGKLYRSVFYSRGEIYLTFDELQSSLLKIKEIIKEEHQS
                     LYLDEIDLLLNKINLFGFHFATLDIRQNSKIHKQVFQDIVSKEQKLFPDNYHQLNEIE
                     KIKVLSNVKSKLTEADFENEITQSTISSIHAMKTIQEHNGELGCNRYIISNNESALNV
                     METLAMFHLCGWVNPAVDVVPLFESVDDLQSADEIMEKLYTNPTYIKHLESRENIQTV
                     MLGFSDGTKDGGYLMANWSIYKAKEMLTAVSRKYGVKVLFFDGRGGPPARGGGKTHKF
                     YASLGSNIENQQIQITVQGQTISSNFGTLDSCRYNLENLLSAGATNQLFSKGEIVLSD
                     SENKVLNELSQLSYEKYKEFKQHTKFIPYLERMSTLNYYAKTNIGSRPSKRNKTEQLD
                     FNDLRAIPFVGSWSQLKQNVPGFFGVGTALKHFEETNQWEQVEHLYQNSLFFKTLLEN
                     SMMALTKSFFPLTAYMRKDEEFGEFWQIIYDEYLESKRLLLKLSGFNELMENYPDGKA
                     SIAIREQIVLPLLTIQQYALLKINQLKSQKNPNEKQIAVYEKIVTRSLFGNINASRNS
                     A"
     gene            complement(3350..3829)
                     /locus_tag="P5335_06330"
     CDS             complement(3350..3829)
                     /locus_tag="P5335_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRZ74529.1"
                     /translation="MSKFRLDEIDHQILDMLIDNTRVPFTDIAKKLLISAGTVHVRVK
                     KMEDAGIVMGSSLTLDYEKLGYSFIAYVGVFLNNTSQTKFVLERINDIPFVTVAHVTT
                     GKFNIFCKIRAKDTKHAKDVIFMVDDIEGVYRTETMISLEESINDKKRLMHTIFQNM"
     gene            complement(3909..5063)
                     /locus_tag="P5335_06335"
     CDS             complement(3909..5063)
                     /locus_tag="P5335_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRZ74530.1"
                     /translation="MDYHSVFEKHKEQDLLGRYISLKNIEPLLGKHLFKNKIDVLGKS
                     VLEKSIYGYQAGNGEYKILLWSQMHGNESTTTKALFDLFNYLDSDAEEVQKWKDKFTF
                     LFIPMLNPDGAELYTRENANKIDLNRDFINLSQPESQLLFSQFSSFKPDFCFNLHDQR
                     TIFAAGKTNNPATVSFLAPSYNEECTINETRQKAINLIAGINMELQKHIPNQVGRFDD
                     GFNRNCVGDTFQQLGVPTVLFEAGHFQNDYVREKTRKFIFISLLRSFSLLYENDIVDN
                     KIDDYLKIPQNNVCFYDIVYRNVKINYDNSIIITNFAAQYKEEIIENQLFFNAYISTV
                     GDLNDIYGHLEYDAQEGLFNDGTDNFPNIEEKADFTIGKSKVFVNGLLIK"
     gene            5369..5785
                     /locus_tag="P5335_06340"
     CDS             5369..5785
                     /locus_tag="P5335_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRZ74531.1"
                     /translation="MLNTQEIVKKLEIIFDYYQLNASSFADKIGVQRSSLSHLLSGRN
                     KPSLEFVIKITECFPEVDLYWFLMNQGYFPKSETKVVAEPKFINTKKNEELKEVKPLV
                     VNSEKQQNILKSTTYIEKVMIFYTNGTVKSYNYSEE"
     gene            complement(5790..6341)
                     /locus_tag="P5335_06345"
     CDS             complement(5790..6341)
                     /locus_tag="P5335_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRZ74532.1"
                     /translation="MKKALYKFIFFKLMGWKISGTLEPSLKKCVMIVVPHTSWHDFYL
                     GIFTRGIVGLEMNFVGKKELFRFPFGFYFRWMGGAPLDRTGGLNRVEAIAKVFSERET
                     FRLAIAPEGTRKKVIEWKTGFYYIALQAKVPIVSIAFDYGKKEVKIGKPLFPTGNILS
                     DIPELEKDFKGVLGKFPGQSYGV"
     gene            6399..7064
                     /locus_tag="P5335_06350"
     CDS             6399..7064
                     /locus_tag="P5335_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRZ74533.1"
                     /translation="MLKKIISYFYPINLYKTTSEISKTLEVTLVNGEIVLDSKNTNYS
                     YGSLQRILRKGLKFIGFDTIKSMNHILVLGVAGGSVIKTLNDEIGFKGKITGIELDEK
                     VIEIANDHFELNKIKNLNLIHHEAFEFVLKTKLNFDLIIIDIFQDTIMPSFLFEAFFQ
                     NRLREILNPGGFILFNTMILKNIDEVRNDNYIRFFESLQFKITRLPRLEEHNELIIVE
                     KIN"
     gene            7146..8102
                     /locus_tag="P5335_06355"
     CDS             7146..8102
                     /locus_tag="P5335_06355"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HRZ74534.1"
                     /translation="MKRYYLILLILLSILLSVGFNSEDKDKNTVKAGKKMQEFVIGIS
                     DFARKQKSDFIVIPQNGSELAFENSNPDKNLSTSYLKAIDGIAIEELFYDEKGKADNY
                     RIENLKKLPKEKKIMVSEFVKNKDDIDKVIQLNQTVNFVPFIRTAENYHYHLIPENIN
                     LENANNITKLSDVQNFLYLINADDFDTKKQLIEAVANTNFDLVLIDLYYYSFSYTKAE
                     LELLKKKKNGGKRLVICYMNVGAAENWRNYWQPDWKLGNPKWLKKKYKGYDNEIYVEF
                     WDANWQKLIYGNEESYTQKVINAGFDGVFLDNVEAYYNLYND"
     gene            complement(8099..>8280)
                     /locus_tag="P5335_06360"
     CDS             complement(8099..>8280)
                     /locus_tag="P5335_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRZ74535.1"
                     /translation="RKKALLDFYKLPMKSLEASEKLEQLRYLEYGKKIKMVETTHIGI
                     GIDTPEDLEKARKMI"
     assembly_gap    8281..8380
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8381..8979)
                     /gene="kdsB"
                     /locus_tag="P5335_06365"
     CDS             complement(<8381..8979)
                     /gene="kdsB"
                     /locus_tag="P5335_06365"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRZ74536.1"
                     /translation="MKIIAVIPARYASTRFPAKLMQDLGGKTVITRTYQAVVKTNLFD
                     DVFVVTDSELIYNEIISIGGKAIRSIKEHESGSDRIAEAVENLDVDIVVNVQGDEPFI
                     NKEPLAAVLEVFRNDTKQEIDLASLMREITDWEMIENPNNVKVIVDQNGLALYFSRAV
                     IPYPREKNVSVRYFQHIGIYAFRKKALLDFYKLPMKSLEA"
     gene            complement(9018..9575)
                     /locus_tag="P5335_06370"
     CDS             complement(9018..9575)
                     /locus_tag="P5335_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HRZ74537.1"
                     /translation="MSIETLISICLGVGLAASAGFRVFVPLFILSLASHLQWIPVNDS
                     WSWIGQTTALIALGIAMIFEILAYYIPFIDSVLDTIAIPLAAIAGTAVMASTLVDLSP
                     LFTWGLAIIAGGGTATVINSATAATRLTSTATTGGIGNPIVSTVETGTATVMSFVSVF
                     IPIIAFVLVLFIFFVGIKLFKKITS"
     gene            complement(9588..>10673)
                     /locus_tag="P5335_06375"
     CDS             complement(9588..>10673)
                     /locus_tag="P5335_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRZ74538.1"
                     /translation="RQVNKHKNTIFIVDESSMISDVNTDSKSADSNSLLDDLMNYVYS
                     GENCKMILLGDTAQLPPINLEVSPALNIETLLAYYNKDVVHIELDEVMRQEENSGILY
                     NATVLREVLKEHFIDSFRFDVKGYKDIVRLTDGYDIQDAINTSYSKDGMEETAFIVRT
                     NKRANQYNQQIRNAILFRESDLSAGDYMMVVKNNYFWLKETDEAGFIANGDIVEVLEI
                     YKIQELYGFKFAKVKIRMVDYPNQKPFETILLLDTITSESPSLTYEEANRLYHEVLKD
                     YENEGASYKKVQKVKENEYFNALQVKFSYAITCHKSQGGQWNTVFVEQPYLPNGMDRD
                     YIRWLYTAVTRAKEKLYLIGFKDENFE"
     assembly_gap    10674..10773
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11002..11763
                     /locus_tag="P5335_06380"
     CDS             11002..11763
                     /locus_tag="P5335_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74539.1"
                     /translation="MILLVIIVVVLALAIYFIAKLINKSTNKKLKPIFSIVLWIVTIL
                     FSYLIYQSIQAPIEFDKLKEKRFQTAVNKMLDIKAAQLAYKTIHGKYTDNLDSLITFI
                     ENENFTIVERKDTSVIDVEKNRAYGLSVDANGTGGYFKDIVVINQLGKVSIKDSLFKG
                     SDRYKNLNTVKVGDIETKITLKAGYIERDDLKIPVFEAKLNKASLLSDQDPSLVAKEK
                     KVVSVEGINGEYILLGSLEEVSMSGNWPKKYGNNE"
     gene            11747..12568
                     /locus_tag="P5335_06385"
     CDS             11747..12568
                     /locus_tag="P5335_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HRZ74540.1"
                     /translation="MEITNNSVIQKSYKKLSIQVSLNGLSFCCRDTLSGQLISFEKID
                     FSNFPKTFTIENSLWKSMLDFSDLTKSYDEIVVLHENNLSTFVPKPLFDEEYKGSYLQ
                     YNTKVFETDYFAVDEMGKHEMVNVYVPYVNLNNYLIDQFGSFEYQHFSSILVSKLLDL
                     SKNDEAQNMFVHVAATHFEIIVIQNQKLLLYNSFEYQTAEDFLYYLLFTAEQLHLNPE
                     SMKLFILGECSENDALYQKAYQYIRNTTLLDVSEQQKTNSFSTQENRTHFILFNT"
     gene            12565..13146
                     /gene="rsmD"
                     /locus_tag="P5335_06390"
     CDS             12565..13146
                     /gene="rsmD"
                     /locus_tag="P5335_06390"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRZ74541.1"
                     /translation="MRIISGKFKGRRLSPPKNLPVRPTTDMSKEALFNILNNHFNFAE
                     LKVLDLFAGTGNISFEFASRGSEPIISVDADYGCISFIKKTTDEFEFNITAIKSDVFK
                     FLEKTKGDFDIIFADPPYGMEQKDFERIVELVFTNELLVEDGMMIVEHSKHTKLENMA
                     AFSFQKNYGGSTFSFFEWESNETAGNESSEEEE"
     gene            complement(13165..13486)
                     /gene="rnpB"
                     /locus_tag="P5335_06395"
     ncRNA           complement(13165..13486)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5335_06395"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         4562 a         2269 c         1974 g         4539 t
ORIGIN      
        1 atgacgatga accctctgat gaggatgacg acgacgacga agattactaa aatacaagga
       61 gctcaaattg agctcctttt ttatttcaaa tatctttcct ttattatctt ctcgtgatgc
      121 ttttgatgtc ctaaagttat aaaaccaatt gcacgaactg aaaccggttt ttctgaagca
      181 gtacccattt gcaataattg ttcctctgtg aatgttttga ataaactaat ggttgcctct
      241 cgaaccgtta taaattcttc tattaaatcg ttcattactc ttttatttgc aaaggcggtt
      301 tctacatact cattttcttc aaaaccgggt aatggggttt tatcttttcg ggcaattcgc
      361 aacgcacgat aagcaaatat tcgctcagca tcaattaaat gttgcacgat atctttgatg
      421 gtccattttc cttcagcata cctaaattca tgtttttcag aaggtatagt cttcaaaaaa
      481 tcgataaaaa aagacaatga tagttctaat tcattcatta aaacaacttc acccaaagcc
      541 tcaatgtaag gtttataaaa attggcatat tcatttgatt gtaaagaaga tgatttcata
      601 ttttatataa tttttatcta tttaagattt ttaaattttt aatttccaaa ctatgccgaa
      661 tttctacttg cattaatatt cccaaacaac gaacgggtaa caattttttc atacactgca
      721 atttgttttt catttgggtt tttttgactt ttcaattgat taattttcaa caaagcatat
      781 tgttgaatcg tcaacaaggg caagacaatt tgttcgcgaa tggcaataga tgcttttccg
      841 tctggataat tttccattaa ttcattaaaa ccggaaagct tgagcaaaag acgtttgctt
      901 tccaaatact catcataaat gatttgccaa aattcaccaa attcttcatc tttccgcatg
      961 taagctgtta agggaaaaaa tgatttggtt aatgccatca tactgttttc taacaaggtt
     1021 ttaaagaaaa gtgagttttg atacaaatgc tccacttgtt cccattgatt ggtttcttca
     1081 aaatgcttca atgcagttcc cacaccaaaa aagcccggta cattttgctt caactgactc
     1141 caagaaccaa caaaaggtat agctcgcaaa tcgttaaagt ctaattgctc agttttattt
     1201 cttttggagg gccgactacc aatatttgtt tttgcataat aattcaacgt actcattcgc
     1261 tccaaatacg gtataaactt agtatgttgt ttgaattctt tatacttttc ataactcaat
     1321 tgtgacaatt cattcaaaac cttattttcc gaatcggata aaacgatttc acctttactg
     1381 aataattgat tagtcgctcc tgcacttaac aaattttcta aattgtaacg acaggaatct
     1441 aacgtcccaa aattggaact gattgtttgt ccctgcaccg ttatttgtat ttgttggttt
     1501 tcgatatttg accctaatga agcgtaaaac ttgtgcgttt ttcctcctcc tcgtgccggt
     1561 ggcccacctc taccgtcaaa aaacaagact ttaacgccgt attttcttga aactgctgta
     1621 agcatttctt tcgctttata aatactccaa ttagccatta aatatccacc atctttagtt
     1681 ccgtctgaga aacctaacat cactgtttga atgttttctc tgctttcaag atgttttata
     1741 tacgtaggat tggtgtataa tttttccata atctcatcgg ccgattgtaa atcgtccacc
     1801 gattcaaata gtggtaccac atcaacagcc ggatttaccc aaccgcacag atgaaacatt
     1861 gccagcgttt ccatcacatt caaagcactt tcattattac tgatgatgta acgattacaa
     1921 cctaactctc cattgtgttc ctgaatggtt ttcatggcat gaatcgaact tatcgttgat
     1981 tgagtaattt cattttcaaa atcagcctcg gttagtttac ttttcacatt tgacaacact
     2041 ttaatctttt caatttcatt tagttgatga taattatccg ggaataactt ttgttcttta
     2101 gaaacaatat cctgaaaaac ctgtttgtga attttactat tctgacggat atctaatgtt
     2161 gcaaaatgaa atccaaataa gtttatttta ttcaacaaca aatcaatctc gtctaaataa
     2221 agtgattgat gttcttcttt aattatctct ttaattttta ataaactcga ttgcaactca
     2281 tcaaatgtca aataaatttc acctcttgaa tagaaaacgg aacgatataa tttaccttca
     2341 atttcagcga taattgtatc aacctctttg aaagtaagtt ttcgttttaa ttttctaata
     2401 tcattgtaat aacatttcaa aatagaagta cgtaatcgtt cagcaacttt gagtgtaatc
     2461 tctgttgtaa caaaaggatt tccatcacga tctcctcctg gccaaaaacc taaatttaat
     2521 attgaattat gaatttcctg tccatcaaaa atgttttttt gaagaaattg aatcatccct
     2581 ccggcagcct cataaaatac attttccaaa taccaaatta aactgattgc ctcatcaaaa
     2641 ggggttggtt tttctttttt gataaatggt gtttttccca attgagctaa taatttttta
     2701 atttgaatca aatcattcac ccgaactgct tcagttaaat ctgtgatgat tcctaaaacc
     2761 gaaccaggat aaaattgtgt gggatgtgcc gttagaacag tgcggacttt aaaattttct
     2821 aaaaactctt tgagttctat cccttttgaa cgttggtctg ccttttcttt catgtctctt
     2881 agcgaacctc ttccctccat gttattcaca attggaaagg ctgcatcttc aatcgcatca
     2941 aataatacta tttggcgttc aacatattga ataaagcgaa acatcaattc aatctttcta
     3001 tcttctgaag gatggtctaa gtattttgaa gcaaataaat tgaaaatttc ttcaggactt
     3061 tgtagattct tgaacccatt ttcacaaact tctgaaaaca agggaagcaa aacacctgta
     3121 ttgtctattg catcaaaagg taaagttata aaaacactat tgtaaatttg atatttagaa
     3181 agcacatttt gatgaaatcg ttcaatttta ggtaaggtat acataaacgc tattttgatt
     3241 actaatttag tgaattttaa attgaaaaat aaaacacaaa gcttgtattt atttcataaa
     3301 aaaaaccctc tcgttcaaag gagagggttt gtactttaag atttcaaaat tacatgtttt
     3361 gaaatatagt atgcatcaaa cgttttttgt cgttaatgct ttcttctaag gaaatcatag
     3421 tttctgttct gtaaacacct tcaatatcgt ctaccataaa aataacatct ttggcatgtt
     3481 tagtatcttt cgcacgaatt ttacagaaaa tattaaactt tccggtcgta acatgagcaa
     3541 ccgttacaaa tggaatgtcg ttgattcgct ccaaaacaaa ttttgtttga gatgtattat
     3601 tcaaaaacac acctacataa gcgatgaatg aatatcctaa tttctcataa tccaaggtta
     3661 atgaagatcc cataacaatt cctgcatctt ccattttttt aacgcgaaca tgaacagttc
     3721 cggcagaaat caataatttt tttgcaatat cagtaaaagg aactcttgtg ttatcaatta
     3781 acatatcaag aatttggtga tctatttcat ctaaacgaaa cttactcatg gcattttatc
     3841 tattagtaat taattattaa attaagacgc aaatgaaata caatttattc aataaataaa
     3901 cctatttatt atttaattag caaaccatta acaaacacct tgctttttcc tattgtaaaa
     3961 tctgcttttt cttcaatatt tggaaaatta tctgttccgt cattaaataa accttcttgt
     4021 gcatcatatt ccagatgtcc gtaaatatca tttaaatctc caacagttga aatataggca
     4081 ttgaaaaaca attgattttc aataatttct tctttatatt gagctgcaaa attcgttata
     4141 ataattgaat tatcataatt tattttgaca tttcgataaa caatatcgta aaaacagaca
     4201 ttattttgcg gaattttcaa ataatcatcg attttattat caactatatc gttttcgtat
     4261 aataaagaaa aactacgtaa taaactaata aaaataaatt ttctggtttt ttctcttaca
     4321 taatcatttt gaaaatgccc cgcttcaaac aagactgtcg gaactcctaa ttgttggaaa
     4381 gtatctccca cacaatttcg attaaaacca tcatcaaatc ggccaacctg atttggaata
     4441 tgcttttgca attccatatt gattccggca ataagattaa ttgctttttg tcgagtttca
     4501 ttaattgtac attcttcatt ataagaagga gccaaaaaag aaacagttgc cggattattt
     4561 gtttttccgg cagcaaaaat tgttcgctga tcatgaagat taaaacaaaa atcgggctta
     4621 aaacttgaaa attgagagaa aagcaactgg ctttccggtt gtgaaagatt gataaaatca
     4681 cgattcaaat caattttatt tgcgttttct cgagtataca actcagcacc atccggattt
     4741 agcattggaa taaaaagaaa tgtaaattta tctttccact tttgaacttc ttcagcatct
     4801 gaatctaaat aattaaacaa atcgaaaagt gcttttgtag tggtactttc attaccatgc
     4861 atttgagacc aaagcaaaat tttatactct ccgtttccgg cttgataacc atatatcgat
     4921 ttttcaagaa ccgattttcc caaaacatca atcttatttt tgaacaaatg cttacccaaa
     4981 agaggttcaa tgttttttaa agaaatgtaa cgacctaaca aatcctgctc tttgtgtttt
     5041 tcgaaaaccg aatgataatc catgatactg ttttttacta ttacaaaagt aaacatcttt
     5101 agaataacaa ttgtaaacga taaaaaaaca ctgcaaattt aactttgtaa acaacttatc
     5161 acatccaata acactactaa attgacaaat gtcattcaaa aatataatta ttatttaaaa
     5221 atataatttc caatatttta taatgtttta attgaagttt aaattccata taaattaatt
     5281 gttttgagta aaattacaat tgtaacaatt cctttttaaa actaaatgat tacatttgta
     5341 acaatctatt ttaccaacaa cattcactat gttaaacacg caggaaattg taaaaaaact
     5401 cgagattatt tttgattatt accaattaaa tgcctcatct tttgcagaca aaattggcgt
     5461 acagcgttca agtttatctc acctactctc cggcagaaac aaaccgagtt tagaatttgt
     5521 tataaaaata acagaatgct ttcctgaagt ggatttatat tggtttttaa tgaatcaagg
     5581 ctatttccct aagtcagaaa cgaaagtcgt tgctgaaccg aaatttatta ataccaaaaa
     5641 aaatgaagaa ttaaaagaag ttaaaccatt agtggttaat tccgaaaaac aacaaaatat
     5701 tttaaaatca actacttaca ttgaaaaagt aatgattttt tatactaacg gaacggttaa
     5761 aagttataat tattcagaag aataactatt tacacaccat aactttgacc gggaaactta
     5821 cccagaactc ctttaaagtc cttttctaat tcaggtatat cggaaagaat atttccggtt
     5881 gggaagaggg gtttccctat tttcacttct tttttaccat agtcaaaagc tattgaaaca
     5941 attggaactt ttgcttgaag tgcaatgtaa taaaatcctg ttttccattc tattactttt
     6001 tttctagttc cttcaggagc gatggctaat cgaaaggttt cgcgttcact gaatactttt
     6061 gcaatggctt ccactctatt caatccaccg gttctgtcta atggtgctcc acccatccat
     6121 cgaaaataaa atccgaatgg gaatcgaaac aattcttttt ttccaacaaa attcatttct
     6181 aaaccaacaa tgcctcgtgt aaaaatgcca agataaaaat cgtgccaact tgtatgtggc
     6241 actacaatca taacacactt tttgagtgat ggttccaaag ttccggatat tttccaaccc
     6301 atcaatttga aaaaaataaa tttatatagt gctttcttca ttgtaaaata ttttagtaaa
     6361 aatacgatat ttctcttatt tttactgaaa tttattccat gctaaaaaaa atcataagtt
     6421 acttttatcc tatcaatctt tataaaacca cttctgaaat cagcaagact ttagaagtga
     6481 ccttagtaaa tggagaaatt gtgttagata gtaaaaacac aaattactcc tatggaagtt
     6541 tacaaagaat tttaaggaaa gggcttaaat ttattggttt tgacactatc aaatccatga
     6601 atcacatttt agttcttgga gtagcgggcg gaagtgtaat taaaacgcta aatgatgaaa
     6661 ttggttttaa aggaaaaatt acaggaattg aattagatga aaaagtaatt gaaattgcca
     6721 atgatcattt tgaattaaac aaaatcaaaa acctcaacct aattcatcac gaagcgttcg
     6781 agtttgtatt aaaaacaaaa ttaaattttg acttaattat aatcgatatt ttccaagata
     6841 ccataatgcc tagtttttta tttgaagctt tttttcaaaa taggttgaga gaaattttaa
     6901 atcctggtgg tttcatactt tttaatacaa tgattctaaa aaacattgat gaagtaagaa
     6961 atgataatta cattcgtttt tttgaatctt tacagttcaa aataacccga cttcctcgat
     7021 tggaagagca taatgagtta attattgttg agaaaattaa ttaaatcctt gtttttggta
     7081 tgtttattgc ctttctgaaa atgagtcaat aaaaaaactt atttttgaaa attcaaaaaa
     7141 ttgttatgaa acgatattat ttaatccttt taattttact ttctattttg ttatcagtag
     7201 gatttaatag tgaagataaa gataaaaaca cagtgaaagc gggtaagaaa atgcaggaat
     7261 ttgtgattgg catttctgac tttgctcgta aacaaaaaag tgattttata gtcattcctc
     7321 aaaatggatc agaattagct tttgaaaatt caaatcccga taaaaacctt tcaacaagtt
     7381 atttgaaagc tatcgacgga attgccattg aagagctgtt ttatgatgaa aaagggaagg
     7441 cagacaacta tcgaattgag aatcttaaaa aacttccgaa ggaaaaaaaa ataatggttt
     7501 ctgaatttgt aaaaaataag gacgatattg acaaagtgat tcaactcaat caaactgtca
     7561 attttgtacc ctttatcaga acagcagaaa attaccatta tcatttaatt ccggaaaata
     7621 taaaccttga aaatgctaac aatataacta aactgagcga tgttcaaaat tttttatatt
     7681 taatcaatgc ggatgatttt gatacgaaaa aacaattaat tgaagcggtt gcaaatacca
     7741 attttgacct tgtattaatc gatttatact attacagctt ttcttatacc aaagccgaat
     7801 tagaattgct taaaaagaaa aaaaacggag gaaaacgatt agtaatttgc tacatgaatg
     7861 ttggtgcagc ggaaaattgg cgaaattatt ggcaacccga ttggaaatta ggcaatccaa
     7921 aatggttaaa aaagaaatac aaaggttatg ataacgagat ttatgttgag ttttgggatg
     7981 caaattggca aaaactaatc tatggaaatg aagaatccta cactcaaaaa gtaattaatg
     8041 ccggatttga cggtgtgttt ttagacaatg tagaagcgta ttacaattta tacaacgatt
     8101 aaatcatttt tcgggctttt tctaaatctt ccggggtgtc aattccaatt ccaatatgtg
     8161 ttgtttctac cattttgatt tttttgccgt attctaaata gcgaagttgt tctaattttt
     8221 ctgaagcctc caaagacttc ataggtaatt tgtaaaaatc caataacgct ttctttcgaa
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcctccaaag acttcatagg
     8401 taatttgtaa aaatccaata acgctttctt tcgaaaagca taaattccaa tgtgttgaaa
     8461 atagcgaaca ctcacatttt tttctctcgg ataaggaatc acagcccgtg aaaaataaag
     8521 agctaaacca ttttgatcca caattacttt tacattattc ggattttcaa tcatttccca
     8581 gtccgtaatt tcacgcatta aggaggctaa atcaatttct tgttttgtat catttctaaa
     8641 aacctctaaa acagcagcca atggttcttt attaataaat ggttcatccc cttgcacatt
     8701 aaccactata tcaacatcta aattctcaac tgcttcagca attctgtcgc tcccactttc
     8761 gtgctctttg atgcttctga ttgcttttcc tcctatagaa ataatttcgt tataaattaa
     8821 ttctgaatct gttactacaa aaacatcgtc aaataaattt gtttttacga ccgcttgata
     8881 cgttcgtgta ataaccgttt tccctcctaa atcctgcatt aatttagcag gaaatcgtgt
     8941 agaagcataa cgggctggta tgacggcaat tattttcata ttaaaaagtg gctgagttac
     9001 taaggtttta agatttttta agatgtaatt tttttgaata atttaattcc tacaaagaaa
     9061 ataaatagta ccaaaacaaa cgctataatt ggtataaaaa cagatacaaa agacatgact
     9121 gtggctgttc ctgtttcaac ggtggaaacg attgggttgc caatacctcc ggttgttgcg
     9181 gttgaagtta atcgtgtggc tgctgtagct gaattgatca ctgtagcagt tcctccacca
     9241 gcaataatgg ccaaacccca agtaaataaa ggactcaaat caaccaacgt ggaggccatt
     9301 acagcagttc ctgcaatggc tgctaatgga atggcaattg tatctaaaac agaatcaata
     9361 aaaggaatat aataggctaa aatttcaaaa atcatagcaa ttccaagtgc aattaatgca
     9421 gtggtttgtc cgatccaact ccaggaatca tttacaggaa tccattgcaa gtgagaagcc
     9481 aaactcagta taaaaagagg tacaaaaacc cgaaaaccag cagatgctgc taatccgact
     9541 ccaagacaaa tactgattaa ggtttcaatg gatatcattt atcaaaatta ttcaaaattt
     9601 tcgtctttaa atccaattag gtataatttt tcttttgctc gcgtaaccgc agtatataac
     9661 caacgaatat aatccctgtc cataccattc ggcaaataag gttgctctac aaaaacagta
     9721 ttccattgtc ctccctgtga tttgtgacac gtgatggcat aggaaaactt aacttgtaaa
     9781 gcattaaagt attcattttc ctttactttt tgtacttttt tatatgaagc accctcattt
     9841 tcataatctt tcaatacttc atgatagagt cgattagctt cttcatacgt caatgaaggt
     9901 gattcactcg taatagtatc caacaacaaa attgtttcaa atggtttttg attgggataa
     9961 tcaaccattc taatttttac tttggcaaat ttgaatccgt ataattcttg aattttatat
    10021 atctccaata cttccacaat atcaccattg gcaataaatc cggcttcatc tgtctctttc
    10081 agccaaaagt aattattctt caccaccatc atataatcac cggcagacaa atcgctttcg
    10141 cgaaacaaaa ttgcatttct aatttgttga ttgtattgat ttgctctttt attagttcgc
    10201 acaatgaaag cggtttcctc cattccatct ttgctgtagg atgtattaat agcgtcttga
    10261 atatcataac catccgttaa acgaactata tctttataac cttttacatc aaaacgaaaa
    10321 gaatcaataa aatgttcttt taaaacttcc ctcaatactg ttgcattata taatatcccc
    10381 gaattttctt cctgacgcat cacttcgtct aattcaatat gaacaacgtc tttgttataa
    10441 taagccaata acgtttcgat attcaaagca ggactaactt ctaaatttat tggcggaagt
    10501 tgtgctgtat cacctaagag tatcatctta cagttttcgc cggaatacac ataattcatt
    10561 aaatcatcca ataatgaatt actgtcggcc gatttcgagt cagtattaac atctgaaatc
    10621 atggaacttt cgtcaacaat aaaaatggta tttttatgtt tattgacttg acgnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngttgaat ttgtagcaaa atcagcaatt
    10801 ttttgaaaaa aaatagtctg ttttaaagta ggattaaaag ggaatgattt ttgtaaaata
    10861 ctgtaaaaca gaggtgaatt catttgcagg ttaattagga gcaataaaaa ttcaaatata
    10921 ctgatgaatt ttatgggaaa tgcttttgag aaaaaaaaaa aattgtagat ttgccttaaa
    10981 ctgaaattaa ataaaattaa aatgatttta cttgtaatta ttgttgtagt tcttgcatta
    11041 gcaatttatt tcattgctaa gttaattaac aaatctacaa ataaaaaatt gaagccaatt
    11101 ttttcaattg tgttgtggat tgttaccata ttatttagtt atttaattta tcaatcaatt
    11161 caagcaccta ttgaatttga taaattaaaa gaaaaacgtt ttcaaactgc tgtaaataaa
    11221 atgcttgata ttaaagcagc acaattggcc tataaaacga ttcatggtaa gtatacagac
    11281 aatttagatt ctctgattac ttttattgaa aacgaaaatt ttacgattgt tgaaagaaaa
    11341 gatacctcag taattgatgt tgaaaaaaac agagcatatg gtttatctgt tgatgcaaat
    11401 ggaacaggtg ggtattttaa agatattgtt gtcattaacc aacttggtaa agtaagcatc
    11461 aaagattctt tatttaaagg ttctgatcga tataaaaacc ttaatactgt aaaagtaggt
    11521 gatattgaaa ctaaaattac tttaaaagca gggtacatcg aacgtgatga tttaaaaata
    11581 cctgtttttg aagcaaaatt aaataaagca tctttattat cagaccagga tcctagttta
    11641 gttgccaaag aaaagaaagt ggtttcagtt gaaggaatca atggtgaata cattttatta
    11701 ggttcattag aagaagtaag tatgtcagga aactggccta aaaaatatgg aaataacgaa
    11761 taatagcgtc atccaaaaaa gttataaaaa gttgtccatt caggtttcac tgaatgggct
    11821 ttctttttgt tgccgtgaca ctttatccgg tcaattgatt tccttcgaaa aaattgactt
    11881 ttcaaatttt cctaaaactt ttactattga aaacagtttg tggaaatcaa tgttagattt
    11941 ttcagattta acaaaatcct acgatgaaat tgtcgttctt cacgaaaaca atttgagcac
    12001 ttttgtgcca aaaccacttt ttgatgaaga atacaaagga agttacctgc aatacaatac
    12061 taaagttttt gaaaccgatt attttgctgt tgatgaaatg ggaaaacacg aaatggtgaa
    12121 tgtatacgtg ccctatgtca atttaaataa ttatttaatc gatcagtttg gctcttttga
    12181 ataccagcat ttttcaagta ttttagtgtc taaattattg gatttatcta aaaatgatga
    12241 agcacaaaat atgtttgttc atgtggctgc tactcatttt gaaattatcg ttattcaaaa
    12301 tcagaagctt ttgttatata attcctttga ataccaaacc gcagaagatt ttctgtacta
    12361 tttattattt accgcagaac aactgcattt gaacccggaa tccatgaaat tatttatttt
    12421 gggtgaatgt tcagaaaatg atgccttgta ccaaaaagca tatcaataca taagaaatac
    12481 tactttgttg gatgtttctg aacagcaaaa aacaaactcc ttttccactc aggaaaaccg
    12541 cacccatttt atccttttca acacgtgaga atcatatccg gaaaattcaa aggccgcagg
    12601 ttatcgcctc ccaaaaacct tcctgttcga ccgactaccg atatgagtaa agaagcattg
    12661 tttaacattt taaacaatca ttttaatttt gccgaattaa aggtattgga tttatttgcc
    12721 ggaaccggaa acatcagttt tgaatttgct tcccgaggca gtgaaccgat tatttcagtt
    12781 gatgctgatt atggatgtat ttcctttatc aaaaaaacta cggatgaatt tgaattcaac
    12841 attacagcca ttaaaagtga tgtgtttaaa tttttagaaa aaacaaaagg cgattttgat
    12901 attatttttg ccgatccacc ctatggaatg gagcaaaagg actttgaaag aatagttgaa
    12961 ctagttttta caaacgaatt gttagtggaa gacggaatga tgatagtaga acattccaaa
    13021 cacactaaac tggaaaacat ggctgctttt tcttttcaaa agaattatgg aggttccact
    13081 ttttcatttt ttgaatggga aagtaatgaa actgcaggta atgagtcttc tgaagaagag
    13141 gaataatcat ttgtccatca ccataaaaaa agcagacctg taagccggat tctgtattcg
    13201 ccaaagcgaa cccttatcat ttatcttgct caatagttac ccattgaatc tagctgccta
    13261 cccttcaacc tcggacgagt agcccttatc cgccgagacg gaacgttgat atacatggca
    13321 tttcaccgca tagagtttac ctggtttcac tacagccgaa ctgtacatac tttctgttgc
    13381 actggtcctc attcgccgag gcgaacggac gggtgttacc cgttatgcta ctctttggtg
    13441 tccggacttt cctcttcgca agcgaagcga taaggcggtc tgcgatgcaa aaataaactt
    13501 aataattcat tattgagcaa aatcctgcgg attaaatttt ccgg
//
LOCUS       DAOTCW010000059        13310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2813_length_13310_cov_43.800528, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000059 DAOTCW010000000
VERSION     DAOTCW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13310
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2813_length_13310_cov_43.800528"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..618)
                     /locus_tag="P5335_06400"
     CDS             complement(22..618)
                     /locus_tag="P5335_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRZ74542.1"
                     /translation="MKKIILSILAVAAFGTANAQDGDATAKGFAKGDMFAEGSFSIRS
                     GDVEDSWSFTPKVGYMINDKIALGGFLAFSGSENNANDKVGRFGLGAFARYYFLSLGA
                     DKAFQAYGELGLGYSSIKSEPAGGGSSTDSAMNANIDLGINYFFTSHWAATFELANIL
                     SYNNTNPENGDNSSDLNVEVNLFNNIFAQPQFGLLYKW"
     gene            complement(832..1374)
                     /locus_tag="P5335_06405"
     CDS             complement(832..1374)
                     /locus_tag="P5335_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74543.1"
                     /translation="MKKLFLSITAVFAFSFTNAQEASTDAGGQTSKGKWLIEANTGNA
                     MLGTTGFYFASSDGESSYNLGLDGGYFIMDDLAIKAGLGYGSDSFSDTSSFSYRLGAK
                     YYVKSMIPVTLDLTGASGDGVENLAGETPMWLGIGAGYAWFLGDNVSIEPGLRYNHSL
                     NEDYTDKGVFQFNIGFALHF"
     gene            1558..2112
                     /locus_tag="P5335_06410"
     CDS             1558..2112
                     /locus_tag="P5335_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRZ74544.1"
                     /translation="MKKSIVIFALFLIGFTNVNAQLLQFGLKGGVNFSNYSGGDIEGF
                     DFNNVTNFHFGVVTELKFFENFALQPELLYSTQGSELESLEEQIKNELGYISIPVLAK
                     FYLTSNKLSLEGGPQFSFLVNERGDVDVYDSNTFDFGVAAGLSYKITKGLFLSGRYVA
                     GLTDVKKEADVKNSLVQFSLGYMF"
     gene            2269..2685
                     /gene="aroQ"
                     /locus_tag="P5335_06415"
     CDS             2269..2685
                     /gene="aroQ"
                     /locus_tag="P5335_06415"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRZ74545.1"
                     /translation="MRIIIINGPNLNLLGKREPDVYGNQTFEDYFVHLQQNYPDIELA
                     FYQSNIEGEIIDKIQEVGFLFDGIILNAAAYTHTSIGIGDAVKAINSPVIEVHISNTF
                     SRETFRHQSYISPNAKGVIVGFGLKSYELALLSFLK"
     gene            2729..5215
                     /locus_tag="P5335_06420"
     CDS             2729..5215
                     /locus_tag="P5335_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRZ74546.1"
                     /translation="MTKLKTFLFFLVVNFSTLSLAQIKGTISDEAGVPLPFVNIYLEN
                     TYVGTTSNEKGQYELNVQNKEQVTIIFQYLGYKTQKHTLDIEKLPYTFNVILKEENYK
                     LNEVVLSSKDNPANAIMRSAITNRKKNSDKTARFTADFYSRGIFRMKNAPKKILGQEI
                     GDFDGALDSTGSGILYLSETVSKITFEKPDKLKERIIASKISGKNNGFSYNTAQATSY
                     DFYDNTVELGVNLISPIADNAFAYYKYTLEGTFYDDNNNLINKIKVTPKRDAEPVFEG
                     HLFIVEDSWAIYGVDLDIKGYRMKEEFLDVMTLKQNFSYNKTNQLWAKNSQSLEFEAG
                     AFGIKFNGKFTYVYSNYDFKEQFEKKTFTREILSFEDNANKKDSTYWNMMRPVPLTDE
                     ENNDYLRKDSIQTLRKSKPYLDSIDAKNNKFKILKILTGYSYRNSFENWNFNYEGIIK
                     LIQFNTVQGWHLSTGISYIKRDEETRKYLTYGADFNYGFSEEKFRTTGFFSTRLDTKT
                     NSLLTLNGGSKVEQFNANYPISNLINTASSIFFEDNYMKLYEKNFVKASYQQEAINGL
                     YLVGSLEYGERKPLYNNSDYVMFNQDKKYTSNNPLNPEDYTTAAIEKHNLFKANINAR
                     INFGQKYLSRPDGKFNIRNEKYPTVFVGYEKGFAGSEENYNYDFLAARVYYEKTIGNK
                     GSFAINLKGGKFFDADGISFVDYKHFNGNQTHVSTADRYLNEFNLLPYYSYSTNNSYM
                     ETHIEHNFEGFIMNKIPLLNKLKSTMVLGYHQLATPDVKPYQEFSIGLDKLGFGKFKL
                     LRVDYVRAYNGSTYLGDGVMFGMKFLGVLE"
     gene            complement(5274..>6266)
                     /locus_tag="P5335_06425"
     CDS             complement(5274..>6266)
                     /locus_tag="P5335_06425"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRZ74547.1"
                     /translation="LARFGKGYIPKPGGDKIGRRRLDTHFEGFINLGAKFRYNREDHF
                     YGVESEGRLKGAYMLLDEASVTGTANIVMASVLAEGTTTIYNAACEPYLQQLCKMLNS
                     MGANITGVGSNLLTIEGVEKLSGCTHRILPDMVEIGSWIGLAAMTRSEITIKDVSWEN
                     LGVIPSVFRKLGITIERRGDDIFIPEHKDGYEIKKDIDGSILNVSDAPWPGFTPDLLS
                     IVLVVATQAKGDVLIHQKMFESRLFFVDKLIDMGAKIILCDPHRAVVIGHNFQSQLKA
                     TTMSSPDIRAGISLLIAALSAKGTSTIQNIEQIDRGYERIDERLRAIGAHIVRV"
     assembly_gap    6267..6366
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6367..6761)
                     /locus_tag="P5335_06430"
     CDS             complement(<6367..6761)
                     /locus_tag="P5335_06430"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HRZ74548.1"
                     /translation="MTYNEKAHEIVHELEYNAERPHLMIPEYGRHLQKLINQATLIED
                     TEERNKAARYIIKVMGSLNPHLRDVLDFQHKLWDQLFIMSDFKLVVDSPYPIPTRDVI
                     NLKPERLEYPQKNPKYRFYGNNIKYMIDVA"
     gene            complement(6772..7056)
                     /locus_tag="P5335_06435"
     CDS             complement(6772..7056)
                     /locus_tag="P5335_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HRZ74549.1"
                     /translation="MDKKTEEFYERLKEELRTTSDWPSIYLYKFIVPTDTIKILAVEN
                     AFDNMGAVINTQQSKTGKFTSISINVSMLSPEHVVEKYIDVSSVEGIISL"
     gene            7289..7828
                     /locus_tag="P5335_06440"
     CDS             7289..7828
                     /locus_tag="P5335_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRZ74550.1"
                     /translation="MSKEIIVITGGPGTGKTTIIDGLIEKGYMCFPEISREIITEARK
                     QGIEQLFLEKPLLFSELLLEGRKKQFQKAEQHPESILFLDRGIPDILAYMHYIGDSYP
                     AFFDENCRQNKYTKVFLLPPWQEIYQCDEERYENFEQAKLIYNHLKETYENYGYQLIE
                     VPQGRLENRILFILEAISR"
     gene            7832..9724
                     /locus_tag="P5335_06445"
     CDS             7832..9724
                     /locus_tag="P5335_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HRZ74551.1"
                     /translation="MQKAESILKKYWGHDEFRSPQKEIISSILSSNDTLGLMPTGGGK
                     SICFQIPALMTDGICLVISPLIALMKDQVDNLMKRDIKALALTGGISSDEVITLLDNC
                     LYGNYKFLYLSPERLQQDWVLEKIKNLPLNLIAIDEAHCVSQWGHDFRPSYLKISVLK
                     TFFPAVPFLALTASATERVKLDIIEQLKFKNPAIFQKSFARENIAYLVVETEDKLFKI
                     EQILKKNPEPSIIYVRNRKSCYEISNQLQQLGIKAAFYHGGLPRRDKTKNMDWWMQEK
                     VQVMVATNAFGMGIDKANVKTVIHYQLPENIENYYQEAGRAGRNGEKSFAVLLVAPSD
                     LAIAQAQFIANLPDNDFLKLSFIKLCNYFQIAYGEGIDEQFAFNLNHFCSLYQFPILK
                     TFAALQFLDRQGIITFTQEFSEKITLQFIIESKEVIRYMSLNPQEEEIILVFLRTYPG
                     IYEIPTAVNLSLVARKSNTEENKIQEILEKLKEKGIVDFIAKNNDATITFNEVREDER
                     TINRVVKYLEAQNNLKKEQLKQVVNYVTNTETCKSRLILEYFGEKNTTECGICSYCIA
                     KKNKSSDTKPLVISIIELLSEKAQTSRELLKVVHCSEELLIFALQELLDDKKIKILAN
                     NKYTIV"
     gene            9726..10673
                     /gene="fmt"
                     /locus_tag="P5335_06450"
     CDS             9726..10673
                     /gene="fmt"
                     /locus_tag="P5335_06450"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRZ74552.1"
                     /translation="MEKLRIVFMGTPDFAVGILDTILKNNYNVVGVITAADKPAGRGQ
                     KMNYSAVKEYAMEKGLNLLQPTNLKDETFLAELKSLKANCQVVVAFRMLPEVVWRMPE
                     FGTFNLHASLLPNYRGAAPINWAIINGETKTGVTTFFIDDKIDTGAIIMAKETPIEEN
                     ENAGQLHDRLMVLGSTTVMETLALIEKGSVSTTIQKDNPNIKTAYKLNRENCKIDWTK
                     PGKEIYNLIRGLSPYPSAWCIIKQDNQEWSVKIHETKLHIETHDFPTGYMQTTKKEMK
                     IAVKDGFIEICSIQFPGKKKMTASELLNGMQFSATCITL"
     gene            10852..11124
                     /locus_tag="P5335_06455"
     CDS             10852..11124
                     /locus_tag="P5335_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRZ74553.1"
                     /translation="MNKSELIDAIAADAGITKAAAKLALESFLGNVGKTLKKGGRVSL
                     VGFGSWSVSKRAARDGRNPQTGKTIKIAAKNVVKFKAGAELEGTVN"
     gene            11245..11805
                     /locus_tag="P5335_06460"
     CDS             11245..11805
                     /locus_tag="P5335_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRZ74554.1"
                     /translation="MISEIIKKGHLLIAEPSILGDLSFNRSVILLADHNAEGSVGFIL
                     NKPLDYSVNDLVPEVDATFTIYNGGPVEQDNLYFIHNIPELIPNSVEISNGIFWGGDF
                     EKTRDLINNGSINKDNIRFFLGYTGWDANQLESEMNANSWIVIENSYKNNILKKSSSD
                     FWKEQIMELGGEYLIWSNAPENPMLN"
     gene            complement(11802..12638)
                     /locus_tag="P5335_06465"
     CDS             complement(11802..12638)
                     /locus_tag="P5335_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRZ74555.1"
                     /translation="MINLNGNLVEADSVLMHNRAFLYGDAVFETLKLVNGKVLFMEDH
                     YFRLMASMRIIRMQIPMNFTLEYLEELIIQLAERNSCSASCRVRLTVYRNDGGYYLPL
                     TRSVSFLISVKPMDNHVYTISNESYEVELFKDFYVTKQLLSTIKSTNRLINTVGSIFA
                     EENGYQNCLLLNEEKNVVEALNGNIFMLIGNQLITPPLSEGCLNGVMRKQILTLAKNT
                     AGIEVIEKAISPFDLQKADELFITNVISGTQPITKYRKKEFESLFAKVLLEKLNELVN
                     LT"
     gene            complement(12686..13078)
                     /locus_tag="P5335_06470"
     CDS             complement(12686..13078)
                     /locus_tag="P5335_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HRZ74556.1"
                     /translation="MDTKIRYEIEFAVNSSPQLLYQYISTPSGLSEWFADNVNSRGEL
                     FTFIWDDSKERARLASKKSNERVKFRWIDDQDKDTDYFFELRISEDEITKDVSLVVVD
                     FAFEDELTESKQLWENQISDLKHVIGSV"
     gene            13225..13298
                     /locus_tag="P5335_06475"
     tRNA            13225..13298
                     /locus_tag="P5335_06475"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13259..13261,aa:Met,seq:cat)
BASE COUNT         4644 a         2242 c         2116 g         4208 t
ORIGIN      
        1 ttttatatca aaaaaatcaa attaccattt gtataataat ccgaattgtg gttgagcaaa
       61 aatgttattg aataaattaa cttctacatt taagtcactt gaattatcac cattttctgg
      121 attagtgttg ttgtaactta aaatattagc taattcaaag gtagcagccc aatgagaagt
      181 aaagaaataa ttgattccta aatcaatatt tgcgttcatt gcactatccg tagaacttcc
      241 acctccagca ggttcagatt ttatacttga ataacctaat cctaattcac cataagcttg
      301 gaaagcttta tccgctccaa gtgataagaa ataataacgg gcaaaagctc ctaaaccgaa
      361 tctaccaact ttatcgttag cattattctc agaacctgaa aaagctaaaa atccacccaa
      421 agcaatttta tcgttaatca tataacctac ctttggggta aatgaccaac tgtcttctac
      481 gtctcctgaa cgaatgctga aactaccttc agcaaacata tcacctttag caaaaccttt
      541 tgctgttgca tctccgtctt gtgcatttgc agttccaaat gcagctacgg ctaaaatcga
      601 taaaataatt tttttcatgt tttaattgtt ttaaaattta taaagcaaaa ttaaaatatt
      661 aaaacacccc gaatttaaag gatttttgta gttgttaaca atgttatcaa cattattgac
      721 aataaatgtt aataacccga aatgatagac actaaattta agacatagtt taatcataaa
      781 aaaacctctc aaaatttaca ttgagaggtt tttaaaatat aaacttatta attagaagtg
      841 taaagcaaat ccaatattaa attgaaaaac acctttgtca gtataatctt cattcaaact
      901 gtgattgtat cgtaatcccg gttcaataga aacattatcc cctaaaaacc aagcatatcc
      961 tgctcctata cccaaccaca ttggtgtttc accagctaag ttttctacac cgtctcctga
     1021 tgctccagtt aaatctaatg tcactggaat catactttta acataatact ttgcccccaa
     1081 tcggtatgaa aaagaacttg tatcagaaaa agaatcagaa ccataaccta aaccggcttt
     1141 aatagctaaa tcatccataa taaaataacc accatccaaa ccaagattat aagacgattc
     1201 tccatcagaa gaagcaaaat aaaacccggt tgtccctaac atggcatttc ctgtattagc
     1261 ttcaattaac cattttcctt tagaggtttg tcctccagca tctgtactag cttcttgggc
     1321 atttgtgaat gagaatgcaa aaactgctgt aattgataaa aataattttt tcatgtttat
     1381 ttgttttaaa agttaatata tcaaatttat gaaaatattt ttcaatgttt acaaggtttt
     1441 tcttagttat taacactttt tttaacaatt cgaacaacta gttttatttt atgaaataca
     1501 atttttagta ataatttcgt ttttaggtag tattgtttat tcactaaaaa aaaatgtatg
     1561 aaaaaatcaa ttgtaatttt tgctttattt ttaatcggtt ttacgaatgt taatgcccaa
     1621 ctactccaat ttggattaaa aggtggggtt aacttttcta actacagtgg aggagatatc
     1681 gaaggatttg attttaacaa tgtcactaat tttcattttg gtgttgtgac cgaattaaaa
     1741 tttttcgaaa attttgctct gcagcctgaa ctactctatt ccacacaagg ttcagaattg
     1801 gagagtctag aagaacaaat aaaaaatgaa ttgggctaca tttctattcc ggtcttagct
     1861 aaattttatt taacgagtaa taagttgagt ttagaaggcg gcccacaatt ttcattttta
     1921 gtgaatgaac gtggtgatgt tgatgtatat gattcaaata cgtttgactt tggtgtcgca
     1981 gctggactaa gttataaaat cacaaaaggc ttatttttat ccggaagata tgtagccggt
     2041 ttaaccgatg tgaaaaagga ggctgatgta aaaaattcat tagttcaatt ttcgctgggt
     2101 tatatgtttt aaacaattta aatggctgac ataaattagt cagccatttt tttaaaagtt
     2161 cattttcttg aaaatcttca caatagcaaa atgttaatat taaattaatg tttttcgaca
     2221 cctgctatct atcacttttt ttggtatttt tacctaaaat cttacaggat gagaatcatt
     2281 attatcaacg gcccgaattt gaatctttta ggcaaacgtg aaccggatgt ttatggcaat
     2341 caaacttttg aagactactt tgttcattta caacaaaact atcctgacat cgaattagcc
     2401 ttttatcaaa gcaatattga aggcgaaatc attgataaaa tacaagaagt cggcttctta
     2461 tttgacggaa ttattttaaa tgccgcggct tacacacata cttccatagg aattggagat
     2521 gctgtaaagg ctatcaattc tccggtaatt gaagtgcata tttccaatac tttttcaagg
     2581 gaaacgttta gacatcaatc ttatatttct ccaaatgcaa aaggcgtaat cgttggtttt
     2641 ggattaaaaa gttatgaatt agctcttttg tctttcctta aataataatc ctttccatac
     2701 aaattaattt attctctacc ttttgattat gactaaactg aaaaccttct tattttttct
     2761 agtggttaat ttttccactc tttctttagc tcaaataaaa ggaactattt cagacgaggc
     2821 aggagttccg cttccttttg taaatattta tttagaaaac acttatgttg gaactacctc
     2881 aaatgaaaaa ggacaatatg aactcaacgt tcaaaacaaa gaacaagtga caattatttt
     2941 ccaatattta gggtacaaaa ctcaaaaaca cactttagat attgaaaaat tgccttatac
     3001 ttttaatgtt attttaaaag aagaaaatta taaattaaat gaagtcgtcc tgagttctaa
     3061 agacaatcct gcaaatgcaa tcatgcgaag tgcaataact aacagaaaaa agaattctga
     3121 taaaacagcc cgatttacgg cagatttcta ttcccgaggc atatttagaa tgaaaaatgc
     3181 tcccaagaaa atcttaggtc aggaaattgg cgattttgat ggtgctttag attcaaccgg
     3241 aagtggaatt ttatatttat cggaaacagt ttccaaaata acttttgaaa aaccggacaa
     3301 gttaaaagaa cgcattattg cttctaaaat aagtggaaaa aacaatggtt tcagctataa
     3361 tacagctcaa gctacttctt atgattttta tgacaataca gttgaattag gcgtaaacct
     3421 aatttctccc attgctgata atgcctttgc ctactataag tataccttgg aaggaacttt
     3481 ttacgacgat aataataact tgattaataa aataaaagta acgccgaaaa gagatgctga
     3541 acctgttttt gaaggccatc tttttattgt agaagacagt tgggctattt atggtgtcga
     3601 tttagacatc aaaggatacc gaatgaaaga agaattttta gatgtaatga ccttaaaaca
     3661 aaattttagt tacaataaaa ccaatcaact ttgggcaaag aattcacaaa gcttagaatt
     3721 tgaagcgggt gcttttggca ttaaattcaa tggaaaattc acttatgttt atagtaatta
     3781 cgacttcaaa gaacaatttg aaaagaaaac gttcaccaga gaaatcttga gttttgaaga
     3841 caatgcaaat aaaaaggatt ccacttattg gaatatgatg cggcctgttc cgctgacaga
     3901 tgaagaaaat aatgactact tacgaaaaga cagtattcaa accttgcgga aatcaaaacc
     3961 ctatttagat tccattgatg cgaaaaacaa taaatttaaa attttaaaaa tcttaaccgg
     4021 ttacagctat agaaattcat ttgaaaactg gaattttaat tatgaaggta tcatcaagct
     4081 aattcaattt aatacggttc agggctggca cctttcaacc ggaatctctt atattaaacg
     4141 ggatgaagaa accagaaaat atttaaccta tggagccgac tttaattatg gcttttcaga
     4201 agaaaaattc agaacaaccg gatttttcag tacccgatta gatactaaaa ccaatagttt
     4261 attaacccta aatggcggaa gcaaagttga acagttcaat gcaaattacc ctattagtaa
     4321 tttaattaat acggccagtt caattttctt tgaagacaat tatatgaaat tgtatgaaaa
     4381 gaatttcgtc aaagcatcct atcaacaaga agcaatcaat ggactttacc tcgttggatc
     4441 acttgaatac ggtgaacgaa aaccgttata taacaactcg gattatgtaa tgtttaacca
     4501 agacaaaaaa tacacctcca ataatccttt aaatccggag gattacacaa cagcagcaat
     4561 agaaaaacac aatttattta aagcaaacat aaatgcccga ataaattttg gacaaaaata
     4621 tttatcgcgt cccgatggaa aattcaacat tagaaatgaa aaatacccaa cagtttttgt
     4681 aggctatgaa aaaggatttg caggcagtga ggaaaactat aattatgatt ttttggctgc
     4741 tcgggtatac tatgaaaaaa cgatagggaa taaaggtagt tttgctatta atttgaaagg
     4801 cggtaaattt ttcgatgcag atggaatttc ctttgtcgat tataaacatt tcaacggaaa
     4861 ccaaacacat gtgagtaccg cggaccgcta tttaaatgaa tttaatttac tcccttatta
     4921 ttcctatagc accaacaata gttacatgga aacgcatatt gaacacaatt ttgaaggatt
     4981 tatcatgaac aaaattccat tgttgaacaa gctgaaatca accatggttt taggttatca
     5041 tcagttagcc actccggatg taaaaccata tcaggaattt agcatagggt tagataaatt
     5101 aggttttggg aaatttaaat tgctacgggt agattatgtg cgggcctata atggcagtac
     5161 ttatttagga gatggcgtaa tgtttggaat gaaatttcta ggcgtactag aatagaaaaa
     5221 acaaaaaaaa gagaagtcaa aacacttctc tttttttata aaataattaa aaactatacc
     5281 ctcacaatat gagctcctat cgctcgtaat cgttcgtcta ttcgttcgta acctctgtct
     5341 atttgctcta tattttgaat ggtgctggtt cctttggcag ataatgccgc aatcaataac
     5401 gaaattccgg cacgaatatc aggggaagac atggttgttg ctttcaattg agattgaaaa
     5461 ttatgtccga taacaacagc acggtgcgga tcacacaaaa taatcttagc tcccatatca
     5521 atcaatttgt ccacaaaaaa caatcggctt tcaaacatct tttgatgaat caacacatct
     5581 cctttggctt gggtcgctac tactaaaaca atactcaata aatcgggtgt aaatcccggc
     5641 caaggagcat cggatacatt tagaattgaa ccatcaatat cttttttaat ttcatatccg
     5701 tctttgtgtt caggtataaa aatatcatca cctcgtcttt caatggtgat tcctaatttt
     5761 ctgaaaacac ttggaatcac acctaaattt tcccaactta catccttaat tgtaatttca
     5821 cttcgagtca tcgctgccaa cccaatccaa ctacctattt cgaccatatc tggtagaatt
     5881 ctgtgtgtgc aaccgcttaa tttttcaaca ccttcaatgg ttaataaatt agaacctact
     5941 ccggtaatgt ttgctcccat cgaattgagc attttacaca attgttgcaa atagggttca
     6001 caagccgcat tataaattgt tgttgttcct tcagctaaaa cggaagccat cacaatattt
     6061 gctgttccgg tgacagaagc ttcatctaaa agcatgtagg ctccttttaa tcgcccttcg
     6121 ctttcaactc cataaaaatg gtcttctcgg ttatatctaa atttagctcc taagttaata
     6181 aacccttcaa agtgagtatc caaacgacgt cttcctattt tatctcctcc cggttttgga
     6241 atatatcctt ttccgaaacg ggctaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnngcaa catcaatcat atacttgata ttgttaccat aaaaacgata ttttggattt
     6421 ttttgaggat attctaatcg ttccggtttt aaatttataa catctcttgt gggtatggga
     6481 taaggactat cgaccactag tttgaaatca gacataataa aaagttgatc ccaaagttta
     6541 tgctgaaaat ctagcacatc tcgcaaatgc ggattcaaac ttcccatcac tttaataata
     6601 tagcgagcgg ccttattccg ttcttcggta tcttcaatta gagttgcctg gttgattaat
     6661 ttttgcaaat gacgtccgta ttccggaatc attaaatgag gtctttcggc attgtattct
     6721 aattcatgca ctatttcatg tgctttttcg ttgtacgtca tttgattttt tttaaagcga
     6781 aattattcct tctacagaag aaacatctat atatttttcc acaacatgtt caggacttaa
     6841 catgctgaca tttatagaga tactggtaaa ttttcctgtt ttagattgtt gcgtattaat
     6901 taccgcaccc atgttatcaa aagcattttc tacagctaaa attttaatag tatcggtagg
     6961 aacaatgaat ttataaaggt aaattgatgg ccaatccgaa gtagttcgca attcttcttt
     7021 taatcgttca taaaattctt ccgtcttctt atccatattc taatttatta taggcaaata
     7081 tacagttttg aatttagatt tgagaaattt gaaattgaat tttgagtttt acgaactaat
     7141 taaaaagcaa ctattaattg aaaataaggc aacaaaattt acttttaaaa gttcaaatct
     7201 atgataaacc acttaatcaa acttaaaagt gaatgaaagt ttgtatttaa actaactatt
     7261 tatctaaatt taccccttta tttacgttgt gagcaaagaa ataattgtga taaccggtgg
     7321 tcccggaaca ggaaaaacaa caatcatcga tggtttaatc gagaaaggat atatgtgttt
     7381 tcctgaaata tcaagggaaa ttattacgga agccagaaaa caaggaatcg aacaattatt
     7441 tttggaaaag ccactactgt ttagtgaatt actactggaa ggaagaaaaa aacagtttca
     7501 aaaagctgaa caacatccgg aatctatact ttttttagat cgtggcatac ctgacattct
     7561 tgcctacatg cattatatcg gcgacagtta tcctgccttt tttgatgaaa attgtcgaca
     7621 aaataagtac acaaaagtgt ttcttttacc gccttggcaa gaaatttatc aatgcgacga
     7681 agaacgatat gaaaactttg aacaagcaaa actcatttat aatcacctga aagaaaccta
     7741 cgaaaattat ggctaccaac tcatagaagt tcctcaaggt cgtttagaga acagaattct
     7801 ttttatctta gaggcaattt caagataaaa catgcaaaaa gcagaaagta ttttaaagaa
     7861 atattggggt cacgatgagt ttcgttctcc ccaaaaagaa atcatatcat ccatattatc
     7921 ctctaatgac acccttggct taatgcctac cggtggtgga aaatcaatct gttttcaaat
     7981 tccggcttta atgacggatg gcatttgtct ggttatttca ccgcttatcg ctttaatgaa
     8041 agaccaagta gacaatttga tgaaacgcga catcaaagca ttggcattaa ccggaggaat
     8101 atcctcagat gaagtcatta ctctgttaga caattgttta tatggaaatt acaagttttt
     8161 gtacctctcc cccgaacgct tacaacaaga ttgggtttta gaaaaaatca aaaatcttcc
     8221 cttaaattta atcgccatag acgaagctca ttgtgtttca caatggggtc acgattttcg
     8281 tccttcctat ttaaaaatca gtgttttaaa aacttttttt ccggctgttc catttttggc
     8341 tttaactgct tctgcgactg aaagagttaa attagatatt attgaacagt taaaatttaa
     8401 aaatcctgct atttttcaaa aatcttttgc acgggaaaat attgcctatt tagtggttga
     8461 aacggaagat aaacttttta aaattgaaca aatcttaaaa aaaaatccgg aaccttctat
     8521 tatatatgta cgcaatcgaa aatcatgtta tgaaatttcg aatcaattac aacaattagg
     8581 aatcaaagct gctttttatc acggaggatt acctaggaga gacaaaacaa aaaatatgga
     8641 ttggtggatg caggaaaaag tgcaagtgat ggtcgccact aatgccttcg gaatgggaat
     8701 tgataaagct aatgttaaaa ccgttattca ttatcaactt cctgaaaata ttgaaaatta
     8761 ttatcaggaa gccggtagag ccggacgtaa tggagagaaa tcttttgccg ttttactcgt
     8821 tgccccctct gatttggcta ttgctcaagc acaatttatc gctaatttac cggacaatga
     8881 ttttttgaaa ctttccttta tcaaattatg caattatttt caaattgcct acggcgaagg
     8941 cattgatgag caatttgcct tcaatttaaa tcacttttgt tcactttatc agtttccgat
     9001 tctcaaaacc tttgccgctt tacaattttt agaccgtcag ggaattataa ccttcaccca
     9061 agaattttca gaaaaaatta ctttacaatt tattatcgaa agcaaagaag taattcgtta
     9121 tatgagttta aatccacagg aggaagaaat tattctggtg tttttaagaa catatccggg
     9181 catttatgaa atcccgactg ctgtaaacct ttctttagtg gcaagaaaaa gtaatacgga
     9241 agaaaataaa attcaagaaa ttttagaaaa acttaaagaa aaaggaattg ttgactttat
     9301 tgctaaaaac aatgatgcta cgattacttt taatgaagtc agagaggacg aaagaaccat
     9361 caatagagta gtaaaatact tggaggctca aaataactta aagaaagaac aactcaaaca
     9421 ggtagttaat tatgtcacaa atacagaaac ctgcaagagt cgtttgattc tagagtattt
     9481 tggagaaaaa aacacaactg agtgtggaat ttgttcctat tgtattgcta aaaaaaacaa
     9541 atcttcagac actaaaccgc tagtaatttc aataattgaa ttactttctg aaaaggcaca
     9601 aacgtcaaga gaacttttaa aagtagtaca ttgtagtgaa gagctactta tctttgcctt
     9661 acaagaactt ttagacgata aaaaaataaa aatattggcc aataataaat atactatagt
     9721 ataaaatgga aaaattacgc attgttttca tgggaacacc tgattttgct gttggcattt
     9781 tggataccat tttaaaaaat aattataatg tagtaggtgt aattaccgct gcagataaac
     9841 cggcagggag aggtcagaaa atgaattatt cagcggttaa agaatatgcc atggaaaaag
     9901 gcttaaacct attacagccc accaacctaa aagacgaaac ttttttagct gagttaaaga
     9961 gcctaaaggc caattgccaa gtggttgttg cttttagaat gctaccggaa gttgtttggc
    10021 gaatgcccga atttggtact tttaatttac acgcttcttt attaccaaac taccgcggtg
    10081 cagcacctat aaattgggct attattaatg gagaaactaa aaccggagtg accacttttt
    10141 ttattgatga taaaattgat accggagcaa ttataatggc taaagaaaca ccaattgaag
    10201 aaaatgaaaa tgccggtcaa cttcatgacc gattaatggt attgggcagt acaacagtaa
    10261 tggaaacatt agctcttatt gaaaaaggat ccgtttcaac aactattcaa aaagataatc
    10321 cgaacatcaa aacagcgtat aaacttaacc gggaaaattg taaaattgat tggactaaac
    10381 cgggaaagga aatttacaac ctaattagag ggctaagccc atacccttcc gcttggtgca
    10441 tcataaaaca agataatcag gaatggagtg tcaaaattca cgaaacaaaa ctacacattg
    10501 aaacacacga cttccctacc ggctatatgc aaacaaccaa aaaagaaatg aaaatagcag
    10561 tgaaagatgg atttattgaa atttgcagta ttcaatttcc gggaaagaaa aaaatgaccg
    10621 cctcagaatt actcaatggt atgcaatttt cagctacctg catcaccctg taggggttga
    10681 aaacactcaa aataagtaaa tatccgatga actgctcgtc gttatcaaca aaaacactta
    10741 agttattaac aaattttacg aaaactcacg aaaacacttg tgcagtaagg aaatcctatt
    10801 aaatttgtta caattaactt tatgtttaac caacaattaa taactattat tatgaacaaa
    10861 tcagaattaa tcgatgctat cgctgcagat gcaggaatta ctaaagcagc tgctaaatta
    10921 gctttagaat catttttagg aaacgtaggt aaaactttga aaaaaggtgg aagagtttct
    10981 ttagtaggtt tcggatcttg gtcagtttct aaaagagctg ctagagatgg aagaaatcct
    11041 caaactggaa aaacaattaa aattgctgct aagaatgtag ttaaattcaa agcaggtgct
    11101 gaattagaag gtactgtaaa ctagtctaat taagtcgata taaagaaaag tcttccactt
    11161 cgggagactt ttttttagat ttttaataaa ataattggat tattgaaaat aattttacta
    11221 aatttaacta aaaattgtag gtaaatgata tcagaaataa tcaaaaaagg tcatttgctc
    11281 attgcagagc cttccatatt aggcgattta tcttttaatc gctctgttat tttattagct
    11341 gatcacaacg ctgaaggttc tgtaggcttc attctcaata aaccccttga ttactccgta
    11401 aatgatttgg ttccagaggt cgatgctact tttaccatct ataatggtgg tccggtagaa
    11461 caagataatt tgtattttat ccataacatc cccgaattga ttccgaatag tgtggagatt
    11521 tccaatggta ttttttgggg tggagatttc gaaaaaacac gtgacttaat taataatggc
    11581 agtattaata aagacaatat ccgtttcttt ttaggctaca caggctggga tgccaatcaa
    11641 ttagaatcag aaatgaatgc taattcatgg attgtaattg aaaatagtta caaaaataat
    11701 atactgaaaa aatcctcctc tgatttttgg aaagaacaaa tcatggaatt aggtggcgaa
    11761 taccttattt ggtcaaatgc ccctgaaaac ccaatgctga attaagttaa attgactaat
    11821 tcattaagtt tttcaagcaa aacttttgca aagagcgact caaattcctt ttttcggtat
    11881 ttagtaattg gctgagttcc ggaaatcaca ttcgttataa ataattcatc cgctttttga
    11941 agatcaaaag gagaaatagc cttttctatt acctcaattc ccgctgtatt ttttgcaaga
    12001 gtaaggattt gtttacgcat tacaccattt aaacatcctt ctgatagtgg tggagtgatt
    12061 aattgattcc caataagcat aaatatattt ccatttaatg cttccactac atttttttct
    12121 tcatttagta aaaggcagtt ttgatagcca ttttcttcag caaaaatact gcccactgta
    12181 ttgattagtc gatttgttga ttttatggtg gaaagcaatt gctttgttac ataaaaatct
    12241 ttgaataact cgacttcata agactcattt gaaatagtat aaacatgatt atccatgggt
    12301 ttaaccgaaa ttaaaaatga aacacttcgg gttagaggca aataatagcc cccatcattg
    12361 cggtaaaccg ttagtcgaac ccgacaagaa gctgaacagg aattacgttc tgccaactgt
    12421 ataattagct cttccaaata ttctaaggta aaattcattg gaatttgcat ccgaatgatt
    12481 cgcatagaag ccatcaaacg gaaataatgg tcttccataa aaagcacctt accattgaca
    12541 agtttgaggg tttcaaaaac agcatcgcca tataaaaatg cccgattatg cattaaaacc
    12601 gaatccgctt caactaaatt tccgtttaga ttaatcataa aaaaagccct aaattaaagg
    12661 gcaaatatac tgtttattct ataaattaaa cggatccgat tacgtgtttt aaatcagaaa
    12721 tttgattttc ccaaagctgt ttagattctg ttaactcatc ctcaaaagca aaatctacaa
    12781 caactaaaga aacgtctttc gtgatttcat cttcggaaat tctcaattca aaaaaatagt
    12841 cggtgtcttt atcttgatca tcaatccacc tgaattttac tctttcattt gattttttgg
    12901 aagctaatct agctcgttct ttagaatcat cccaaataaa agtaaataat tcacctctgg
    12961 aattcacatt gtcagcaaac cattcagaca aaccggaagg agtagaaata tattgatata
    13021 gcaattgcgg agaagaattt accgcaaact ctatttcata tcgtattttt gtatccataa
    13081 acttaatttt tgagcaatat attgaatatt aattcaaaaa aaaagcgaat ttataaataa
    13141 aaataatttc ttattttatg tttgtaaggt ttgtgattat ttctatattt gcacccgcgt
    13201 tgcgacatag gtaacaagaa aaatggcgag gtagctcagt cggttagagc gttggattca
    13261 taacccaaag gtcgggagtt caaatctccc tctcgctaca aggttacaac
//
LOCUS       DAOTCW010000060        13138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_2873_length_13138_cov_51.199725, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000060 DAOTCW010000000
VERSION     DAOTCW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2873_length_13138_cov_51.199725"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="P5335_06480"
     CDS             <1..348
                     /locus_tag="P5335_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRZ74557.1"
                     /translation="KRMKKVFVHPFSKWLFRWLHPDLGVKLAQYLSVKNKLISGAEDV
                     KFLGEENEWLVQYTKRKLTQKHYDYFVFGHRHLPLQLAIGENSTYVNLGDWIGYFTYG
                     VFDGEKFELKKFD"
     gene            complement(349..2037)
                     /gene="recJ"
                     /locus_tag="P5335_06485"
     CDS             complement(349..2037)
                     /gene="recJ"
                     /locus_tag="P5335_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRZ74558.1"
                     /translation="MRWTLKTKPEAHLVSSLAAQLKVDERIATLLVQRGISTYEQARQ
                     FFKPTLADLHNPYLMKDMDKAVNRIETAMANQENILVFGDYDVDGTTAVSLVSAYLRS
                     YYPNVATYIPDRYDEGYGISFKGIDFADDNGFSLIIALDCGIKSIDHVEYANERKIDF
                     IICDHHRPGDNLPKAIAVLDPKRDDCYYPYDELCGCGVGFKLIQALAQNRGQSIYDLL
                     PYLDLVATAIAADIVPMTGENRILAKFGLEVINKNPRPGIKALIQNLKKKILTITDVV
                     FIIAPRINAAGRIKHGNHAVELLTEFNLEQAEQFAAEIEGYNVDRKELDKQITVEALA
                     QIEENQEKEKFTTVVFHEKWHKGVIGIVASRLTETYYRPTLVFTKSGDKLAASARSVK
                     DFDIYNALEKCAEHLEQFGGHMYAAGMTLKEENYAAFKEAFEKVVQESIHPDLLIPEI
                     SIDAEIELKEINPKLIRLLNQFEPFGPENMTPVFLSKKVVDTGYGKTIGQSDEHLKLF
                     VKQNNSEGYGVIGFGLGNKLERTKDKTPFDLVYCIDENEFNGNVTVQLRAKDLA"
     gene            2213..2536
                     /locus_tag="P5335_06490"
     CDS             2213..2536
                     /locus_tag="P5335_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRZ74559.1"
                     /translation="MYQTSYNKNWYAMTDAVVVKTLCASLKQMRLNQNLSQEELAERC
                     GLSRITISRMETGQAINLLTMVQLMRALGKLDLFNYLNEEPEISPMKVMEERAKYRKK
                     ASPSK"
     gene            2539..3828
                     /locus_tag="P5335_06495"
     CDS             2539..3828
                     /locus_tag="P5335_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HRZ74560.1"
                     /translation="MNTLAAIKIWNHRVGAILWDESKNVGFFEFDKDFVKLPFDLSPL
                     ILPIEDARKGRRVYSFPFLNYETFKGLPGLLADSLPDKFGNQVIDAWLAQQGRSAQLF
                     NPVDRLCYIGKRGMGALEFEPESGQLATPSNPLEIEALVQFSREVLDQRATFKTNLQS
                     ENGFSDLLQVGSSAGGARAKAIVAYNPSTGEVRSGQIDGLAGYDYWLIKFDGVTNHQL
                     GDPKGYGNIEYAYYLMAQAAGITMMESQLKKENQRAHFMTKRFDRLNNQKLHTQTLCG
                     IAHFDYNMPGAYSYEQAFQIMRQMQLPYTDMEELFRRMVFNIMARNQDDHTKNISFLM
                     NPEGKWSLSPAYDITFAYNPSNFWLKSHQMTINGKKDDLNLTDVLTVAKNVHLKKPTQ
                     LIHECREAIVRWGEFADAAFINSEQQAEIKGFFNLLM"
     gene            complement(3886..4563)
                     /gene="rsmI"
                     /locus_tag="P5335_06500"
     CDS             complement(3886..4563)
                     /gene="rsmI"
                     /locus_tag="P5335_06500"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRZ74561.1"
                     /translation="MSKLYIVPTPIGNLEDITFRAIKVLKEVDCILAEDTRNSGKLLK
                     HFEIGTHMQSHHMHNEHKTVEGLIKRLQSGETIALISDAGTPAISDPGFLLTRACVEN
                     NIEVECLPGATAFVPALVNSGLPNDKFVFEGFLPDKKGRQTRFLALADEVRTMIFYVS
                     PHKLTKTLAEFVNYFGADRPVSVSRELSKLHEETVRGSSAEVLKHFENKPAKGEIVVV
                     VGGKSIK"
     gene            complement(4564..5508)
                     /locus_tag="P5335_06505"
     CDS             complement(4564..5508)
                     /locus_tag="P5335_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74562.1"
                     /translation="MKKKLEADLISIAHRVLKLKGKEDVVQLHQEAQKLYEKLAILRF
                     YEENINVLKEEISAEELEEKLNTVAVAQPIVEMEEVTVEPTLVQETLIEETLQEEPVA
                     EVPTVEEVVSETVEELTEEEIVPEPETVAATEEVLEEESKTETIEPLFNPIFELSIEE
                     EEEKTAPKLEQKQILFEDLLGSDYKEPDFIKVNDVPKEVERASEMSFEKAEETFVPEI
                     KAEVFVAKMEHKIASLNDTLIKGINIGLNDRIAFVKHLFAGSNEDFNRVVSQISTYDS
                     FEEAQSFVEDMVKPDYNNWGGKEDYAARFMNLVEKKFA"
     gene            5611..6231
                     /locus_tag="P5335_06510"
     CDS             5611..6231
                     /locus_tag="P5335_06510"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRZ74563.1"
                     /translation="MFLENTVNHKEQFGWIEVICGSMFSGKTEELIRRLKRAQFAKQK
                     VEIFKPAIDTRYHDEMVVSHDANEIRSTPVPAAANIRILAQGCDVVGIDEAQFFDDEI
                     VTICNDLANSGIRVIVAGLDMDFKGNPFGPMPALMATAEYVTKVHAVCTRTGNLANYS
                     FRKADNDKLVMLGETEEYEPLSRAAYYHALREQAEHKTENTNKPKS"
     gene            6235..8676
                     /locus_tag="P5335_06515"
     CDS             6235..8676
                     /locus_tag="P5335_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HRZ74564.1"
                     /translation="MNLVIKDIITLLNASFSGSHSENSIENVSLDSRSLQNNQQTLFF
                     CLVGPNNNGHHYIAELIEKGVRNFIVSEKVEPVQDVNFLLVDNTLTALQKFASFYRKK
                     FDFPVIGITGSNGKTIVKEWLNFLLSPDYNIIRSPKSYNSQVGVPLSVIGINEQHNLG
                     IFEAGISTVHEMEHLEPIIEPTIGILTNIGSAHDEGFENLGQKIKEKLKLFKHVHVLI
                     YHKNKSIDAFLNHDIKTFSWSSSDKTADVLVHPKTTNQQTTLNVTYQETCFKIKIPFS
                     DEASIENVIHCLMVLFYFNYDFDTIQNRLSLLYPIEMRLKVKKGINNTMLIDDSYSSD
                     FQSLKIALDFLENQKQHHKKTLILSDIFQSGLENDELYSRVSQMIISNKISRVIGIGE
                     TISQYKSKFLNGMTFGSQTDFLAHFDNLTFENETILIKGARNFHFEEIVSLLEEKTHE
                     TVLEINLNSLSHNLNFYKAKLKPDTKMMVMVKAFSYGNGGLEIAKLLEYLNVDYLGVA
                     FADEGIALKSNGIHLPIMVLNPETTSFEAIIQHHLEPEIYSLKGLNAFLKIAKQKLLK
                     NYPIHLKIDTGMHRLGFDEIHLEELITILKNCKTVQVKSILSHMATSDDLTHHEFALE
                     QIEKFNRISTSIRKELEISPIRHILNTSGISNYPQAQFDMVRLGIGLYGISNDTHEQL
                     YLENVGTLKSIISQIRTIQKGESVGYGRRFIAERETKIATIPIGYADGIRRSWGNGIG
                     YVMINNQKSTIVGSICMDMLMVDVTEIPCVEGNEVIILGDNLSVSMMAEKLGTIPYEI
                     LTGISQRVKRIFYRE"
     gene            8746..9153
                     /gene="mscL"
                     /locus_tag="P5335_06520"
     CDS             8746..9153
                     /gene="mscL"
                     /locus_tag="P5335_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRZ74565.1"
                     /translation="MFKEFKNFIMTGNVIEFAVAVIMAGAVGAVVNGFVNDIVMPIVG
                     MLTGGVDFSEMKVVLQAAIVESEGVAAVPEVAIRWGAWVNTLVNLVIVGFVMFMIIKA
                     YNKMKKPKVEAPAAPAGPTQEELLAEIRDLLKK"
     gene            9297..10286
                     /locus_tag="P5335_06525"
     CDS             9297..10286
                     /locus_tag="P5335_06525"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRZ74566.1"
                     /translation="MRVAVVGATGMVGEVMLQVLAERNFPITELIPVASEKSIGKEID
                     FKGTNYKVVGLQTAVDLHPDIALFSAGGETSLSWAPKFAAVGTTVIDNSSAWRMDPTK
                     KLVVPEINASELTKDDKIIANPNCSTIQMVMVLAPLHKKYNIKRVIVSTYQSITGTGV
                     KAVQQLENEYAGVKGEMAYPYPIHRNAIPQCDVFEDNGYTKEEMKLVRETQKIMGDRS
                     IAVTATAIRIPVVGGHSEAVNVEFSNDFDVNEVRSILHNTAGVTVQDNLDTKTYPMPI
                     YAQGKDDVFVGRIRRDESQSNTLNMWIVADNLRKGAATNTIQIAEYLVANKLV"
     gene            10347..10682
                     /locus_tag="P5335_06530"
     CDS             10347..10682
                     /locus_tag="P5335_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74567.1"
                     /translation="MKKKIAIVNLYLMMAVLFTILFQSLHTYRHLVTDYFDKTEVCHH
                     SHDGFSHQHADDDCSVCDFHFWFYVKPDVLTYRFDFPIKPVPYLLQEITQVSSFSGSL
                     FSHRGPPTV"
     gene            10761..>13138
                     /locus_tag="P5335_06535"
     CDS             10761..>13138
                     /locus_tag="P5335_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ74568.1"
                     /translation="MRYFITLFFLLCFGFSGFGQFTLRGKITNEKNQPLSGSHVHTNY
                     KNVASDPIGEYEIKGLPKGELRVYISYLGYKTCDTLLNIKEDYVLNVKLTPSETTLKE
                     VTVKQTSTKVSNSISVQQINTERIERSSSQTLAEALKEVSGVSLLKTGSSIIKPMING
                     LHSSRVPIFSNQVRLEDQQWGAEHAPNFDINAAGKITVIKGASGLQYSGDAVGGLVII
                     EPLTVTKDTLFGKTILSGLSNGRGGVISSSIHQGKPAGWRWNALSTFKYLGDKEAPDY
                     VLSNTGNREFNFSGDISFVKKEYEVLAFYSVYLAEIGILSASHIGSANDLYNSIENQV
                     PNVINDFTYAIINPKQTVEHHLAKLNYKQKIDDSSSLSTFYSFQFNHREEYDLRRTDN
                     ANKAALDLELATHTLQTDYNKAWEKWTLKTGINALYQNNFANPKTDVRPLIPSYVKFD
                     GGLYGVGNYQIHEELALEMGIRYDFSTIEATKYYQKSRWEERDYDADFADFIIGEVGN
                     QWLTKPRFTFHNFSGSFGIRKTYASDWTWLNNLSLSNRNPNPSEFFSDGLHHSTGQIE
                     LGDLRLLQEQSIKYSTSLLKKWNAVSVEFNPYFHWISNFMFLKPIDFETTIRGAFPVW
                     EYQQTDARLWGIDLRSQWQINPTWKHLFSVAYVNGENLIENQPLIDMPPLNLSTSVQY
                     KKSSWNELVLELQYELVLQQNRYPNYNFDTTILVDGELSPVTVDISTPPNGYQLFNFY
                     SEMKVKLFPKILTTLAFSVQNILNTSYRDYLNRQRFFADETGRNIQLQLKFN"
BASE COUNT         4018 a         2465 c         2505 g         4150 t
ORIGIN      
        1 aaacgcatga aaaaagtgtt tgttcatccg ttttcgaaat ggcttttccg ttggttgcat
       61 cccgatcttg gggttaaact agctcaatat ctttctgtca aaaacaaatt aatttccggt
      121 gccgaagatg tgaaattttt aggcgaagaa aacgagtggt tggtgcaata taccaaacga
      181 aaactcaccc aaaagcatta tgattacttt gtctttggtc atcgtcattt gcctttgcaa
      241 ttagcgatag gtgaaaattc cacttacgta aatttaggcg attggattgg ttattttacc
      301 tatggagttt ttgacgggga aaaatttgag ttgaaaaagt ttgactagtt acgctaagtc
      361 tttcgctcgc aattgaaccg ttacatttcc attgaattcg ttttcatcaa tacaatagac
      421 taaatcaaac ggggttttat ctttggttcg ttccaatttg tttcccaacc cgaaaccaat
      481 tacaccatat ccttccgaat tattttgctt gacaaataat ttcaaatgct cgtcactttg
      541 cccgatggtt tttccgtaac cggtatcgac aaccttttta gacaggaaaa cgggcgtcat
      601 gttttcgggg ccaaacggtt caaattggtt taaaagtcgg atgagttttg ggttaatttc
      661 tttcaattct atttcagcat caatcgaaat ttcgggaatc aataaatccg gatgaatgga
      721 ttcctgcact actttttcaa aagcctcttt gaaagccgca taattttctt ctttcaaggt
      781 catgccggcg gcatacatgt gtccgccaaa ctgttctaaa tgttccgcac atttttccaa
      841 ggcattgtaa atatcaaaat ctttcacgga acgggctgag gcagcgagtt tatcaccgct
      901 tttggtaaaa accaaggttg gtctgtaata ggtttcggtt aaacgggaag ccacaattcc
      961 gatgacacct ttgtgccact tttcgtgaaa gacaaccgtt gtaaacttct ctttttcctg
     1021 attttcttca atttgagcga gggcttccac cgttatttgt ttgtccaatt cttttcggtc
     1081 cacgttatac ccttcaattt ctgccgcaaa ttgttcggct tgctccaggt tgaattcggt
     1141 taataattct accgcgtggt tgccgtgttt aattctaccg gcggcattaa ttcggggagc
     1201 gatgataaac accacatcgg ttatggtcag tatttttttc ttcaggtttt gaatcaaggc
     1261 ttttatgccg ggtctagggt ttttgttaat gacttccaat ccaaatttgg ctaaaattcg
     1321 attttcaccc gtcatgggaa cgatatcagc agcaatggct gtagctacca aatctaaata
     1381 cggcagtaag tcatatatgg attggcctct gttttgagct aaagcctgaa tgagtttgaa
     1441 acccacgccg cacccacata attcgtcgta gggatagtaa caatcgtctc ttttagggtc
     1501 taaaacggca atagctttgg gcaaattatc tcccggtcgg tggtggtcgc atatgatgaa
     1561 gtcaattttt ctttcgttgg catattcaac atggtcgatt gatttgattc cgcaatcgag
     1621 agcaatgatg agcgagaacc cattgtcgtc tgcaaagtca attcctttga aagaaatgcc
     1681 atagccttcg tcgtatcggt caggaatgta ggttgccaca ttcggataat agcttcttaa
     1741 ataagccgaa accaaagaga ctgccgtagt gccgtcgaca tcataatcgc cgaaaaccaa
     1801 aatattctct tggttcgcca tcgctgtttc aattcgattg accgctttgt ccatatcttt
     1861 catcaagtat ggattgtgta aatccgctaa agtaggtttg aaaaattgac gtgcctgctc
     1921 gtaggtagaa attcctcttt gaaccaacaa cgtagcaata cgctcgtcca cctttagctg
     1981 agcggctaag gatgaaacta ggtgagcctc cggttttgtt tttaatgtcc aacgcatagt
     2041 ataaaatttt gaatggcggt tagtatttca tgatagaaac aaatttaatg tgttttcgtt
     2101 taataccatt actttgagga gagaaaaaat tgggagaggg gggggggtaa actcgaaaaa
     2161 aagaagtaca tttgtatatt aaatgtttca tttttgtgtt aaattgactt ttatgtatca
     2221 aactagttac aataaaaatt ggtatgcgat gactgatgcc gtagtagtaa agacactatg
     2281 tgccagtttg aaacaaatgc gtttaaacca gaatctttcc caagaagagc tggcagaacg
     2341 atgtggttta agtcgaataa ccatcagccg tatggaaacc ggtcaagcca ttaatttgtt
     2401 aaccatggtg cagctcatgc gggctttagg caaattagac ctcttcaact acttgaatga
     2461 agagcccgaa atcagtccga tgaaagtaat ggaagaacga gcaaaatacc gtaaaaaagc
     2521 ctccccttcc aaataaccat gaatacccta gcggctataa aaatatggaa tcatcgggtt
     2581 ggtgctatcc tttgggatga atcaaaaaat gttggttttt ttgagtttga taaagacttt
     2641 gtcaaacttc cgtttgattt atctcccctt atcttgccta tcgaagatgc ccgaaaaggt
     2701 cgtcgggtgt attcttttcc ctttttgaac tatgaaacct ttaaaggatt acccggttta
     2761 ttagccgata gtttgccgga taaatttggg aatcaggtta tcgatgcatg gctggctcaa
     2821 caaggccgtt ccgcccaact tttcaatccg gttgaccgcc tttgttacat tggcaaacgc
     2881 ggaatgggag ccttggaatt tgagcccgaa tcagggcaac tcgccacccc ttcgaacccc
     2941 ttggaaattg aagccttagt gcaattttcc cgagaagtgt tggaccaacg agccaccttc
     3001 aaaacaaatt tacaatccga aaacggattt tctgacctcc ttcaagtggg aagttctgcc
     3061 ggcggtgccc gagcaaaagc cattgtagcc tacaaccctt ccactggaga agtccgctcc
     3121 ggtcaaattg atggtttagc aggctatgat tattggctta tcaaatttga cggtgtaacc
     3181 aatcatcagt tgggcgatcc aaaaggatat ggaaacattg aatatgccta ttacttaatg
     3241 gctcaagctg ccggaataac catgatggaa agtcagttaa aaaaagaaaa tcagcgggcc
     3301 cattttatga ccaaacggtt tgaccgcctt aataatcaaa aattgcatac gcaaaccctc
     3361 tgtggaatag ctcactttga ttacaatatg cccggtgcct attcctatga acaagccttt
     3421 caaataatgc ggcaaatgca gctgccctat accgacatgg aagaattgtt ccgaagaatg
     3481 gtttttaaca tcatggccag aaaccaagat gatcacacca aaaacatctc atttctcatg
     3541 aatccggaag ggaaatggtc actttcacct gcttatgata ttacatttgc ttataatcct
     3601 tccaattttt ggttaaaatc acatcaaatg accatcaatg gaaaaaaaga tgatctcaac
     3661 ttaaccgatg ttttgactgt tgcaaaaaat gtacatctta aaaaaccgac tcaacttatt
     3721 catgaatgcc gagaggctat tgtaagatgg ggagaatttg cagacgctgc ttttattaat
     3781 tccgagcaac aagcagaaat caagggtttt tttaacctat taatgtaact taattgtttc
     3841 attgtgattg ttttttgagc ttattgtatc aaaaaagttg tatttttatt tgattgattt
     3901 gccgcctacc actactacaa tttctccttt agcgggttta ttttcaaagt gttttaggac
     3961 ttccgcggat gaaccgcgaa cggtttcttc atgaagttta gacaattctc tggacacaga
     4021 aaccggtcta tctgccccaa aatagtttac aaattcagcg agtgtttttg ttaatttatg
     4081 cggagacaca taaaaaatca tcgttctgac ttcgtcggct aaggctaaaa aacgggtttg
     4141 tctacctttt ttatccggaa ggaacccttc aaacacaaac ttatcattag gcaatccgct
     4201 attcactaaa gccggcacaa aagccgttgc tccgggcaaa cattctactt cgatgttgtt
     4261 ttcgacacaa gccctagtca acaaaaaacc cggatcggaa atggccggtg ttccggcatc
     4321 ggaaatcaaa gcaatcgttt ctccggattg taaccgctta atcagtcctt ccaccgtttt
     4381 gtgttcgttg tgcatgtggt ggctttgcat gtgcgtacca atttcgaaat gcttcaataa
     4441 tttaccgctg tttcgggtgt cttctgccaa gatacaatct acttctttga gcactttaat
     4501 ggctctaaaa gttatgtctt ccaggtttcc gatgggtgtg ggaacgatgt acagtttgga
     4561 catttaggca aattttttct ctaccaaatt cataaaacga gcggcataat cttctttccc
     4621 tccccaatta ttatagtcgg gttttaccat atcctccaca aaactttgtg cttcttcaaa
     4681 tgaatcatag gtactgattt gagaaaccac gcgattaaaa tcctcgttac ttcccgcaaa
     4741 taaatgtttt acaaaagcaa ttcggtcatt tagtccaatg ttaatgcctt tgattaaagt
     4801 gtcgtttaac gatgcgattt tgtgctccat tttagccaca aaaacttctg cttttatttc
     4861 cggcacaaaa gtttcttccg ctttttcaaa ggacatttca gaagctcttt ccacttcttt
     4921 cggaacgtcg ttgactttta taaaatccgg ttctttataa tctgagccca ataagtcttc
     4981 gaacaaaatt tgtttttgct ctaattttgg agctgttttc tcttcctcct cctctattga
     5041 taattcaaaa atcggattga aaagaggttc tattgtttct gttttacttt cttcttccaa
     5101 cacctcttcc gttgctgcta ctgtttcagg ttcaggaaca atttcttctt cggttaattc
     5161 ttccacagtt tccgaaacta cttcctcaac agtcggtact tctgctacgg gctcttcttg
     5221 cagtgtttct tcaattagag tttcttgaac aagcgttggc tcaaccgtaa cttcttccat
     5281 ctccacaata ggttgggcaa cggctactgt atttaatttt tcttccaatt cttcggcaga
     5341 aatttcttct ttcaatacat taatattttc ttcataaaaa cgaagaatgg ctagtttttc
     5401 atataatttt tgagcttcct gatgcaattg tactacatct tctttgcctt ttaattttaa
     5461 aactcggtgg gcaatgctga ttaaatcggc ttctaacttt ttcttcataa cttttaaaat
     5521 tatattgact atagcgttta gaattttata aatttgttac tgatacaaaa taacataaaa
     5581 atatgttgtt caccgctaga aaagtagaaa atgtttcttg aaaataccgt aaatcataaa
     5641 gaacaatttg gttggattga agtcatctgc ggatcgatgt tctcgggaaa gaccgaagag
     5701 cttattcgaa gactgaaaag agctcaattt gccaaacaaa aagtggaaat ttttaagcct
     5761 gccatcgata cgcgttatca cgatgagatg gtggtttcgc atgatgccaa tgaaattcgt
     5821 tcgactcccg ttccggcggc tgctaatatt cgtattttgg ctcaaggttg tgatgtagta
     5881 ggcattgacg aagctcagtt ttttgatgat gaaattgtaa ccatttgcaa tgatttggcc
     5941 aattcaggaa ttcgcgtaat tgtggccggt ttagacatgg attttaaagg aaatccattt
     6001 ggaccaatgc ccgctttgat ggcaacagca gaatatgtca ctaaagtgca cgccgtttgt
     6061 acccgcacag ggaatttagc caattacagt tttcgaaaag cagataatga taaattggtt
     6121 atgctaggtg aaacggaaga atatgaacct ttaagcagag ccgcctatta tcatgccctt
     6181 cgcgaacaag cggaacacaa aacggaaaac acaaacaaac ctaaatccta actggtgaac
     6241 cttgttataa aagatattat tacgttgctc aatgcctctt tttcaggcag tcattctgaa
     6301 aatagtattg aaaacgtatc cctcgacagt cgctccctgc aaaataacca acagactttg
     6361 tttttttgtt tggtgggtcc caacaacaac ggacatcatt atattgctga attgattgaa
     6421 aaaggtgttc gaaattttat tgtttccgaa aaagttgaac ctgttcaaga tgtcaatttt
     6481 ttactcgttg acaatacatt aactgcactt caaaaatttg cttcttttta tcgtaaaaaa
     6541 tttgatttcc ctgtgattgg tattaccgga agtaacggaa aaaccatcgt caaagaatgg
     6601 ttaaattttt tattaagtcc tgattataat attatccgca gtccgaaaag ttacaattca
     6661 caagtgggtg ttccgctgtc ggtcattggc ataaatgagc aacataactt agggattttt
     6721 gaagccggaa tttcaacagt tcatgaaatg gagcatttgg agcctatcat tgaacccacg
     6781 attggaattt taactaatat tgggtcagcc cacgacgaag gttttgaaaa tttaggtcag
     6841 aaaatcaaag aaaaattaaa attattcaag catgttcacg tgcttattta tcataaaaac
     6901 aaatcgattg acgccttttt aaatcatgac attaaaacat tttcatggag ttccagtgat
     6961 aaaacagctg atgttttagt acatcctaaa accacgaatc agcaaaccac cttgaatgtt
     7021 acttatcagg aaacctgttt taaaataaaa attccgtttt cagatgaggc ctcgattgaa
     7081 aatgtaattc attgtttgat ggttttgttt tatttcaatt atgattttga cactattcaa
     7141 aatcgactat cccttttgta tcctatcgaa atgcggttaa aagtcaagaa aggcataaac
     7201 aacactatgc tgattgatga cagttacagt tctgattttc aatcgttaaa aattgcatta
     7261 gacttcttag agaatcaaaa acagcaccat aaaaaaacgc ttatcctttc agatattttt
     7321 caaagtggat tggagaatga tgaattatat tcccgggttt cgcaaatgat tatttccaat
     7381 aaaatcagcc gtgtaattgg catcggtgaa accatttccc agtataaatc caaattctta
     7441 aacgggatga cttttggctc acaaaccgat tttttggctc attttgacaa tttaactttt
     7501 gaaaatgaaa ccattttaat caagggagcc cgaaactttc attttgaaga aattgtttcc
     7561 ttattagaag aaaaaacaca tgaaaccgta ttagaaatta atctaaattc cctttcacat
     7621 aatttaaatt tttataaggc taaactaaaa cccgacacca aaatgatggt gatggtaaaa
     7681 gccttcagtt atggaaacgg tggtttagaa atagccaaat tattagaata cctcaacgtt
     7741 gattatttag gtgtagcttt tgccgacgag ggaattgcgt tgaaaagtaa tggaattcat
     7801 ttaccaatta tggttttgaa cccagaaact acgagttttg aagctatcat acaacatcat
     7861 ttagaaccag aaatttacag tttgaaagga ttaaatgctt ttttaaaaat tgccaagcaa
     7921 aaacttctta aaaattatcc gattcacctc aaaattgata ccggcatgca ccggttaggt
     7981 tttgatgaaa ttcatttgga agaattaata acgattctaa aaaattgcaa aacggttcag
     8041 gtaaaaagta tactgtctca catggcgacg agtgatgatt taactcacca cgaatttgct
     8101 ttggagcaaa ttgaaaaatt caaccgtatt tctacctcta tccgtaagga attagaaatt
     8161 agtccgattc ggcatatttt aaatacttca ggaatatcca attatccgca agcacaattt
     8221 gacatggttc gattagggat tggtctttat ggaatttcta atgatactca tgagcaactt
     8281 tacttggaaa atgtaggcac cttaaaatcc attatttccc aaatcagaac cattcaaaaa
     8341 ggagaaagcg taggatacgg ccggagattt attgcggaaa gagaaaccaa aattgccacg
     8401 attccgattg gctatgctga tggaatcaga agaagctggg gaaatgggat tggctatgtc
     8461 atgataaata atcaaaagtc tactattgta ggcagtattt gcatggacat gttaatggtt
     8521 gacgtaactg aaataccctg tgtggaagga aatgaagtta ttatcttagg cgacaacctc
     8581 agtgtgagca tgatggctga aaaattaggt actattcctt atgaaatcct gaccggaatt
     8641 tcacaacggg taaaaagaat tttttacaga gaataaagaa atttattgca actgttaaaa
     8701 ttttaactaa cttcgtttta acaaaccttt taaaacctaa ttattatgtt caaagaattt
     8761 aagaatttta ttatgaccgg caatgtgatt gaatttgccg tagctgtaat catggccgga
     8821 gccgttgggg ctgtggttaa tggttttgtg aatgatattg ttatgccaat agtaggaatg
     8881 ctaaccggtg gtgttgattt ttctgaaatg aaagttgttt tacaagctgc tattgttgaa
     8941 tcggaaggtg ttgcagccgt tccggaggta gctatccgat ggggagcatg ggttaacacc
     9001 ttagttaact tggttattgt tggttttgtc atgtttatga ttatcaaagc ctacaacaaa
     9061 atgaaaaaac caaaagtgga agcacctgct gcaccagccg gaccaacaca agaagaatta
     9121 ttagctgaga ttagagattt attgaaaaaa tagtcgttcc gctacattat atattctaac
     9181 tatactcccg ttaaaagcgg gagtcttttt tttacaaaaa aatcaaaatt ttgtgaaacc
     9241 aatctatttc caatgaatat tctatatttt tgcaattcaa atctaaaaat tcacacatga
     9301 gagttgcagt agttggtgct accggaatgg ttggcgaagt tatgcttcag gttttggccg
     9361 aaagaaattt tccgataacg gagttaattc cggttgcttc agaaaagtca attggtaaag
     9421 aaatcgattt taaaggaacc aactataaag tagtaggttt acaaacagcg gttgatttgc
     9481 atccggatat tgctttgttt tccgccggag gagaaacctc tttatcttgg gcaccaaaat
     9541 ttgcagcagt tggcacaacc gtcatcgaca attcatccgc ttggcgaatg gatccgacta
     9601 agaaactagt ggttccggaa atcaatgctt ccgaattaac caaagacgat aaaatcatag
     9661 ctaatcctaa ctgttctact attcagatgg tgatggtttt ggccccgctt cataaaaaat
     9721 ataacattaa acgagtaatt gtctcaacat atcaatccat taccggaaca ggggtaaaag
     9781 ctgttcaaca attagaaaat gaatatgctg gtgtgaaagg cgaaatggct tatccttatc
     9841 cgattcaccg caacgctatt ccccaatgtg atgtttttga agataacggt tacaccaaag
     9901 aagaaatgaa attggttcgc gaaacccaaa aaatcatggg agacagaagc attgccgtta
     9961 ccgcaaccgc gattagaatt ccggttgttg gcggtcacag tgaagctgta aatgtagaat
    10021 tttcgaatga ttttgatgtg aatgaagttc gttcgatatt acacaatact gccggagtta
    10081 ccgttcagga taatttagac actaaaacct atccgatgcc tatttatgca caaggcaaag
    10141 atgatgtttt tgtaggccga attcgccgtg atgaaagtca gtccaacacc ctaaacatgt
    10201 ggattgtggc ggataattta cgcaaaggag cggcaaccaa taccattcaa attgcagagt
    10261 atttggtagc taacaaacta gtttaacaaa agattaaaag taatttaagc tcctttttag
    10321 gggctttttt gctatctttg ttgttgatga agaaaaaaat agctatagtc aacctctatt
    10381 taatgatggc ggtattgttt acaatactgt ttcaatcgtt gcatacgtac cgacatttag
    10441 taactgatta ttttgataaa actgaagttt gtcatcattc acatgatggc ttttcacatc
    10501 aacatgccga cgatgattgt tcggtttgtg attttcattt ttggttttat gtcaaaccgg
    10561 atgtattaac gtatcgattt gatttcccga ttaaacccgt tccttatctt ttacaggaaa
    10621 tcactcaggt ttcttccttt tcaggttctc ttttttcaca ccgaggtccg ccaaccgttt
    10681 aaaattatcg aaactaaaat acaatcattt gttccaagaa cgaatgattg tcagtgttta
    10741 caattttttg aaccagaaaa atgcggtatt ttattactct tttcttcttg ctatgttttg
    10801 gtttttcagg ttttgggcaa ttcacgttac gtggaaaaat taccaatgaa aaaaaccaac
    10861 ctttatcagg aagccatgtc cacactaact acaaaaatgt agccagtgat cctattggcg
    10921 aatacgaaat caagggtttg cctaaaggtg aattgcgggt ttatatctcc tatttaggat
    10981 acaaaacctg tgacacactc cttaacataa aagaagatta tgttttgaat gtcaagctta
    11041 ctccatctga aactactttg aaggaagtca cggtcaaaca aacttctaca aaagtcagta
    11101 acagtattag tgtgcagcaa atcaatacgg aaagaattga aagaagtagc agtcaaacct
    11161 tggcagaagc tctcaaagaa gtatcgggtg tttccctttt aaaaaccggg agcagtatta
    11221 tcaaaccgat gattaatgga ttgcacagta gtcgggttcc tattttttcc aatcaagttc
    11281 gattagaaga tcaacaatgg ggagctgaac atgctccaaa tttcgacatt aatgctgccg
    11341 gaaaaataac cgttattaaa ggagcctccg gattacagta tagtggtgat gctgtaggtg
    11401 gattagttat tatagaaccc cttacggtga caaaagacac ccttttcgga aaaactattt
    11461 tgagtggtct ttctaatgga cgcggtggcg taatcagttc aagcattcac caaggaaagc
    11521 cggcaggatg gcggtggaat gccttgagta cttttaaata tttaggcgat aaggaagctc
    11581 ctgattatgt tttatccaat accggaaatc gtgaatttaa tttttcggga gatatttcct
    11641 ttgtaaaaaa ggagtatgaa gttttggcct tttacagtgt ttaccttgct gaaataggga
    11701 tattaagtgc ttcacacatt ggtagtgcga atgatttgta taattcgata gaaaatcagg
    11761 ttcccaatgt aattaatgat tttacttatg ccatcatcaa cccaaaacaa acggtagagc
    11821 atcatttggc aaaattgaat tacaaacaaa aaattgatga ttccagttcc ttatcaactt
    11881 tctattcctt tcagttcaac catcgggaag agtatgattt gcgaagaact gacaatgcca
    11941 acaaagcagc tttagattta gaattggcaa ctcatacgct tcaaacagat tataataaag
    12001 catgggaaaa atggacttta aaaaccggta tcaatgctct ttatcaaaac aattttgcca
    12061 atccgaaaac ggatgtaaga cccttaattc cttcctatgt taaatttgat ggcggacttt
    12121 acggggttgg taactatcaa attcatgaag agttagcttt agaaatgggt atccgttatg
    12181 atttttctac cattgaagcc actaaatatt atcaaaaatc ccgttgggaa gaacgtgact
    12241 atgatgctga ttttgctgat tttattattg gtgaagttgg aaatcagtgg ctcactaaac
    12301 cacgttttac ttttcataat ttttcaggga gtttcggaat tcgtaaaaca tacgcttctg
    12361 attggacatg gttaaacaac ctcagccttt ccaatagaaa tccgaatcca tccgagtttt
    12421 tcagtgatgg cttacaccat tctaccggtc aaattgaatt gggtgatttg cgtttgctac
    12481 aagaacagtc gattaaatat tctacttctc tcttgaaaaa atggaatgcg gtgtccgttg
    12541 aatttaatcc ttattttcat tggatttcta acttcatgtt tctgaaacct attgatttcg
    12601 aaacaacgat aagaggggct tttccggttt gggaatacca acagaccgat gcccgattat
    12661 ggggaattga tttacgcagt caatggcaaa ttaaccctac atggaaacat cttttttcag
    12721 tagcttatgt caatggagaa aatttaatcg aaaatcaacc tttgattgat atgcctcctt
    12781 taaacctttc cacgtcggtt caatataaaa aatcatcctg gaacgagttg gtattggagt
    12841 tacagtatga gttggttctt cagcaaaatc gctatccaaa ttacaatttt gacacaacta
    12901 ttcttgttga tggcgaatta tcgcctgtga cggttgacat cagcacaccg ccaaatggtt
    12961 atcaactttt taacttttat tccgaaatga aagttaaatt gtttcctaaa attttgacta
    13021 cccttgcttt ttctgttcaa aatattttaa acacttctta ccgggactat ttgaaccggc
    13081 aacgtttttt tgcggatgaa accggaagaa acattcaact acaattaaaa tttaatta
//
LOCUS       DAOTCW010000061        12575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3090_length_12575_cov_36.415735, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000061 DAOTCW010000000
VERSION     DAOTCW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3090_length_12575_cov_36.415735"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2399)
                     /locus_tag="P5335_06540"
     CDS             complement(<1..2399)
                     /locus_tag="P5335_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ74569.1"
                     /translation="MKKLLFLFCLIGISGFNYSQTITIKDKQSGKPIDLVTLSSEDPK
                     VFTITNTGGQATISAFKGAKKIEIRMPGFKTVIKSFAEIESDGFEIQLEASNLNLEEV
                     VLSGTRWRQLSSNVPSKIISISPAEIALQNPQTAADLLSISGKVYLQKSQQGGGSPMI
                     RGFATNRLLYTVDGVRMNNAIFRSGNIQNVINQDPFTIENTEVLFGPGSVIYGSDAIG
                     GVMSFQTLTPQLSLTDKSFITGKAVTRTSSANKEKTGHFDVNVGWNKWAMVTSFSTWD
                     FDHLRQGSHGPDEYIKPYYVQRQDSIDVVVTQDDPLLQIPSAYSQLNLMQKIRFKPTE
                     KWDFQYGFHYSETSTYGRYDRHNRVRNGTARYAEWNYGPQKWLMNNLNVNYNGNAKLY
                     DQMTLRLAQQSFEESRIERSLNNDNRNTQTEKVKAYSVNLDFNKAINQKNTLFYGLEY
                     VFNDVHSSGYLTDISTSENTTGPSRYPQSTWSSIAAYVNDEFKVTNQLSVQTGLRYNY
                     YKLDSDFTNNLAFYPFPFDSAKINDGAITGSLGAVYRTLNSWVFSTNFGTGFRSPNVD
                     DVGKVFDSAPGTLTVPNPDLKAEYAYNVDLGFAKVISNVIKVDVTGFYTILQNAMVKR
                     DFQLNGESSIIYDGEVSQIQAVQNAAKAKVYGIQLGLEVNLPAGFSISSDLNYQKGDE
                     ELDDGTTSPLRHAVPLFGMSSLNYKAEKLNLQFYSSYQAKRNFEDLAFEEQGKDEIYA
                     KDSNGNNYSPSWYTLNLKGMYVLTKTFSVNAGVENITDQRYRLYSSGISAPGRNYVLS
                     F"
     gene            2946..>3157
                     /locus_tag="P5335_06545"
     CDS             2946..>3157
                     /locus_tag="P5335_06545"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRZ74570.1"
                     /translation="MLSDIEIAQATPIQHIKEIASKLNISNDDLEYYGKYKAKLPLHL
                     IDYKNLNKSKLILVTAITPTPAGEGKT"
     assembly_gap    3158..3257
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3258..4118
                     /locus_tag="P5335_06550"
     CDS             <3258..4118
                     /locus_tag="P5335_06550"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009411613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRZ74571.1"
                     /translation="PFASIAQGTNSIIATRMGLSLGDYVVTEAGFGADLGAEKFFNIK
                     CAVSGLKPNAVVLVVTIKALRHHGGANKAEYNSPSESRVKSGLDNLAKHIENMKLFGF
                     NPLIAINAFPNDSEEEIQLVKELCQCYQTQAIVAKGYTDGGKGMTELATQLVKLVEEN
                     TGSFKPLYSFEQSPEDKISAIATKIYGAEGVEFSVKAKSQLQQIKSFGFDNFPICMAK
                     TPKSFSDDESKRGRPTGFTVKVREFEIASGAGFIIPILGEMMRMPGLPTIPNAENIKI
                     DDQGVISGLS"
     gene            4183..5070
                     /locus_tag="P5335_06555"
     CDS             4183..5070
                     /locus_tag="P5335_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HRZ74572.1"
                     /translation="MKRVGFLFIVLIYTHNILGQGFFEGSDIISKTYSQRWELDSIDK
                     KGTFRLVSYKSIYVAPARWSSNPNEKPQSENPNNSATETIDYDIVEVKFQLSLKTKLL
                     QSIFWGKGDLWVGYTQIAHWQIYNEELSRPFREINYEPEIIFKYPLHIKAFTGEFKSI
                     GFSFTHQSNGRELPYSRSWNRIIFDLSYEIESWIVSVRPWIRLSDGKKDDNPAITDYI
                     GDGELNLSYSYNRHQFYTVITHPFNQFQGGSVQLNYIFPIKGHVRGHLQAFQGYGETL
                     IDYNNKQTTIGIGVSFANW"
     gene            5228..5638
                     /gene="mce"
                     /locus_tag="P5335_06560"
     CDS             5228..5638
                     /gene="mce"
                     /locus_tag="P5335_06560"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRZ74573.1"
                     /translation="MNLSHIEHLGIAVKSLEEAIPFWENMLGLKCYAIEEVKEQKVKT
                     AFFQIGQSKIELLESTDPDGPIGKFIEKKGEGIHHLAIAVKGIEENLAELDQKGVQLI
                     DKTPRKGAEGLDIAFIHPKSTHGVLLELCENKNL"
     gene            5650..7209
                     /locus_tag="P5335_06565"
     CDS             5650..7209
                     /locus_tag="P5335_06565"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRZ74574.1"
                     /translation="MESTDKIKLLLDKRIEAKLGGGQKRIDTQHAKGKLTARERIELL
                     LDEGSFEEFDMFVTHRCTNFGLENQKYLSDGVVTGFGTIDSRLVYVFSQDFTVFGGSL
                     SETFANKICKVMDMALKMGAPIIGINDSGGARIQEGVTSLAGYAEVFQRNIMASGVIP
                     QISAVFGPCAGGAVYSPALTDFIMMTKQNSYMFVTGPKVVKTVTGEVVTDEELGGAMV
                     HGTKSGVTHFVAEDEQEGLLLIRKLLSYLPQNNLEDPPLSVCADPIDRLIDELNTIIP
                     ENPNKPYDVKDVIHAIADENEFFEVHRHYAPNIVVGYAKFNGMPVGIVANQPSYLAGV
                     LDNNASRKAARFVRFCDAFNIPLVTLVDVPGFLPGTTQEYGGIIIHGAKLLYAYGEAT
                     VPKVTVILRKAYGGAYDVMSSKHLRGDINYAWPSAEIAVMGPKGAIEILYQKEYMQIE
                     DLEERAKFIAEKEVEYKEKFANPYNAASYGYIDDVIEPRNTRFRIIRALQSLATKKVV
                     NPPKKHSNLPL"
     gene            7211..7603
                     /locus_tag="P5335_06570"
     CDS             7211..7603
                     /locus_tag="P5335_06570"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HRZ74575.1"
                     /translation="MNTLLLVQQPVSHKAAAEFAKLDPYGIGMTMVAMAVVFLSLIIL
                     YVTFKNIAKLFAYDFKAKPQIKVEGIELSNTKSQQTLELGAAIAMALHLYQNELHDHE
                     NALLTIKKVNGNYSPWSSKIYGMRKELK"
     gene            7610..8053
                     /locus_tag="P5335_06575"
     CDS             7610..8053
                     /locus_tag="P5335_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74576.1"
                     /translation="MKRYKFKINGNSYDVKIDSIEENQAIVDVNGVSFEVELEKKSSI
                     AKTPKLVRSVAVPSTDSSQAVAKTSSPSAPKGGGTIKSPLPGVILKVHVKVGDVVNLS
                     QELITLEAMKMENSIQADKAGVVQAIHFNTGESVMEGDVLLTIGS"
     gene            8057..9211
                     /locus_tag="P5335_06580"
     CDS             8057..9211
                     /locus_tag="P5335_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRZ74577.1"
                     /translation="MDNFWSFLAEHLHQFFTYTAFANFTIGHGVMILIGLIFIYLAIF
                     KEYEPMLLVPIGFGMLIGNIPFYPGLQIGIYEQGSVLNYLYFGVMKGIYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQLGIFGAYMAALALGFLPEQAGAIGIIGGADGPTAIF
                     LSSKLAPELMGAIAISAYSYMALVPVIQPPIMRLLTTSKERLIRMKPPRVVSRNEKIM
                     FPIFGLLLTTFIVPSGLPLLGMLFFGNLLKESGVTKRLADTAKGSLIDIVTILIGLTV
                     GASTQATTFLTGQSVGIFAIGAVSFIIATFGGVMFCKIFNLFLKEGNKINPLIGNAGV
                     SAVPDSARVSQVIGLEYDRTNHLLMHAMGPNVAGVIGSAIAAGVLLSFLG"
     gene            complement(9208..9453)
                     /locus_tag="P5335_06585"
     CDS             complement(9208..9453)
                     /locus_tag="P5335_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74578.1"
                     /translation="MKLISFLALPENEQYRILFEKGAVVAERLEKPVIKKLYSLHTFF
                     VEVHFHYGTEEILFKKVFKAGELLDNYLNDFKIPKST"
     gene            complement(9569..10990)
                     /locus_tag="P5335_06590"
     CDS             complement(9569..10990)
                     /locus_tag="P5335_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74579.1"
                     /translation="MKLRIIAFLFLCVFGNTFAQTPEWGEKFDFDPKNELEPKFVLTD
                     NYNYYLLTVVNKYGMMSSNQIILRKFDQKNALMETITHPFPVLEGGGTLHNFLGSYAI
                     GTEKAVVFTQSYSGKSKRDEVYQHVFDKTSKKFTSTMLMGFPIVSNSKSGDVKVKQSQ
                     NGAFFVVNYKAANSKKEPEVNNLMVLNSTTLDVAWKKEVTFANEFFTGNLVATNSGKV
                     VLVRQPYGYKLDNYLVAVSETEQTDLKVGESIKIHEPIAISIGTQDYLLAMNYPSKGV
                     RRGDFGDLMLYDLSSGAILQNNKIDGFNSITKIEEVNFRTILMQNNEIAIFAEAKVDA
                     TPKPTPGSTGFPVEKHTFGPSYLITLNFEGILKSIKPIPVSTSGEADLYHSFGLVNAN
                     GDLFVQTGLENGFFKWNYLFNNEKIARSISFGYYDNNNLERSVAYVNQLVHYFPDTQK
                     FLFAKITTTDKTQMSMGTFTGVK"
     gene            complement(10997..12190)
                     /locus_tag="P5335_06595"
     CDS             complement(10997..12190)
                     /locus_tag="P5335_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74580.1"
                     /translation="MKLNTFFASLFVFVSTISIYAQNISSENIDFLILKQPKETIDVA
                     NRLLKVTVTSPYNVTADDVIKQSKVDHQAALDNYTTVVANSEKEFQQKLKDYDQEVIK
                     AKEKFELESAEFKKLSLLERMSMTDQGKNPRLVNPTKPVYVKPLPPVYQEPNLNNYII
                     VDNNVLSSQINVEGFSKEGNYLDVAVDIRGVQFQDNAGQTFANQPTTLVVKVNGVEKI
                     NTKFFQEFKFVSSSPSNNINKPLEEKNHLNKVVAFLNQYLNDNYGFQSIKKTVKLDKV
                     KNKGQYDDLEKAHIYVTTNLKKLQPSEPERSAAAVTAMQKGIDIWLETLKKVEFKKDS
                     KSDFNAKIGEYIYFNLIRLNLAMNKKSEAEKYLNELQENLISVKLSYDEQNELKALEN
                     EIYKK"
     gene            complement(12354..>12575)
                     /locus_tag="P5335_06600"
     CDS             complement(12354..>12575)
                     /locus_tag="P5335_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74581.1"
                     /translation="AVTSLVNDLLNKKKDILPTAATNKKDYDAKMTSIRNLFPEKMKK
                     LISADQMKLLTALIPKSNTSSNVLSKMLF"
BASE COUNT         4084 a         1945 c         2219 g         4227 t
ORIGIN      
        1 ctaaacgaaa gaacataatt ccttccggga gcagaaatac cggagctata taaacgataa
       61 cgttggtcag ttatgttttc aactccggca ttaacactga aggtttttgt taaaacatac
      121 attcctttta aatttaaagt ataccaagaa ggagaataat tatttccatt agaatccttt
      181 gcataaattt catctttgcc ttgttcttcg aaagctaaat cttcaaaatt gcgtttagct
      241 tgataagaag aataaaattg taagtttaat ttttcagctt tataattcaa acttgacatt
      301 ccaaataagg gaactgcatg acgtaacgga ctcgttgtgc catcatctaa ttcttcatct
      361 cctttttggt aatttaaatc agatgaaata ctgaatccgg caggaagatt tacttctaaa
      421 cctagttgaa taccgtaaac tttagcctta gcggcgtttt ggactgcttg aatttgactt
      481 acttccccat cataaatgat actgctttcc ccattaagtt ggaaatctct ttttaccatc
      541 gcattttgta aaatggtata aaatccggta acatcaactt taataacatt actgataact
      601 ttggcaaaac ctaaatcaac attataggca tattctgctt ttaaatcagg atttggcact
      661 gttagagttc cgggagctga atcaaacact tttcccacat catcaacatt aggtgatcga
      721 aatccggttc cgaaattagt actgaaaacc catgaattta aggtacggta aacggctcca
      781 agacttccgg ttatggcacc atcattgatt ttagcagaat caaaaggaaa aggataaaaa
      841 gccagattat ttgtaaaatc tgaatccaat ttataataat tgtatcgaag accggtttgc
      901 accgataatt gattcgttac tttaaattca tcattcacat aggcagcgat agagctccaa
      961 gtagattgcg gataacgtga tggtccggtt gtattttcac tcgttgaaat atcggttaaa
     1021 taaccactcg aatgaacatc attaaaaaca tattccagtc cgtaaaacaa agtattcttt
     1081 tgatttattg ctttattaaa atcaagatta acagaatagg ctttgacttt ttctgtttgt
     1141 gtatttctat tatcattatt taaagaacgt tcgattctac tttcttcaaa tgattgttga
     1201 gctaggcgta atgtcatttg atcatacagt ttagcattgc cgttgtagtt aacattcagg
     1261 ttattcatca accatttttg aggaccataa ttccattcag cgtaacgggc tgttccgttt
     1321 ctaacccggt tatgacgatc atatcgtcca taagttgagg tttctgaata gtgaaaacca
     1381 tattgaaaat cccatttttc agtaggtttg aatctaattt tttgcattaa attcaattgc
     1441 gaataagcgg aaggaatttg taataatgga tcatcttgtg ttaccactac gtcaatactg
     1501 tcttgccgtt gcacataata aggttttata tattcatccg gaccatgact tccttgacgt
     1561 aaatgatcaa aatcccaagt actgaaactt gtcaccattg cccatttgtt ccaaccaaca
     1621 ttcacatcaa aatgtcccgt tttttcttta ttagcagatg aagttcgggt aacagctttt
     1681 ccggttataa atgatttatc cgttaaggaa agttgaggag tcaaggtttg aaaactcatc
     1741 acgccaccta tggcatcgct tccataaatt acggaacctg ggccaaaaag tacttcagtg
     1801 ttttcaatcg taaaaggatc ttggttaatt acattttgaa tattaccaga ccgaaagata
     1861 gcattattca ttcgcacccc atctactgta taaagtaatc ggttggtcgc aaaacctcta
     1921 atcattggac ttccaccacc ttgttgactt ttttgtaaat atactttacc tgaaatactt
     1981 agtaaatctg ctgctgtttg cgggttttgt aaagcaattt cagccggtga aatagaaata
     2041 attttggagg gaacattgct ggagagttgc ctccatcttg taccagaaag aaccacttcc
     2101 tctaggttta aatttgaggc ttctaattgt atttcaaaac catcggattc aatctcagca
     2161 aagcttttaa ttacagtttt aaaaccgggc attcgtattt ctattttttt agctcctttg
     2221 aaagctgaaa tagtagcttg gccacctgtg tttgtgatgg taaatacttt tggatcttca
     2281 ctagacaagg tgactaaatc aatcggtttt cccgattgtt tatctttaat agtgatggtt
     2341 tgagaatagt tgaatcctga aattccaata agacaaaata aaaataatag ttttttcatt
     2401 gtttttaatt aaatttcaaa atataaacta ttgaaaatga tagttgtaat tcatcataaa
     2461 tgaaatttga taaaaggctt aaatataaat cttttacagc aaaaaacaga taagtagtaa
     2521 ccaatagata atcaaaattc attttttaat tgttacatta ataaaaatga gttgaattga
     2581 tttttacaac taaaattttt aaatagctta aacaaaaaaa ttatagaaat caattttgca
     2641 ttttgtttga aaagttgtag caatgatgat gccgaaatgc cggattattc aacttttttt
     2701 aatgaaaaag caccaagttt gtatggtaaa ttcaattcac aaactatata ttggggattt
     2761 tgtcttgata atcaaatgtt ttatttagtg cttagagaag gctttttgga atcagaatta
     2821 aggtcctata tttttgatta agtgattatt ttagatttta taaatttatt tcagaagtga
     2881 attgataatt atcatactta tacaaataca atattcttac cttcgatacc ataaaattaa
     2941 atcatatgct ttccgatatc gaaattgccc aagcaacacc cattcaacac ataaaagaaa
     3001 tagcttcaaa actaaatata tctaatgacg atttagaata ttacggcaaa tataaagcta
     3061 aattaccact tcacttaatt gattataaaa atctgaataa gagtaaattg attcttgtta
     3121 cggcaattac tccaacacct gcaggtgaag gtaaaacnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnncct tttgccagta ttgctcaagg tacaaattca attattgcca
     3301 ctcgaatggg tttatcttta ggtgattatg ttgtcacgga agccggtttc ggtgccgatt
     3361 taggtgcgga aaaatttttc aatataaaat gtgctgtttc cgggcttaag ccaaatgctg
     3421 ttgttttagt ggttacaatt aaagcattgc gtcatcatgg tggagcaaat aaggctgaat
     3481 ataattcgcc ttcagaaagt agggtaaaat ccggattaga taacttggcg aaacatattg
     3541 agaatatgaa acttttcgga tttaatccat taatcgctat aaatgcattt ccaaatgatt
     3601 cagaggaaga aattcaattg gtaaaagagt tgtgtcaatg ttatcaaact caggcaattg
     3661 tcgcaaaagg ttatacagat ggaggtaaag gaatgactga attagccaca caacttgtta
     3721 aattagtaga agagaatacc ggtagtttca aaccactgta ttcctttgaa caatcaccgg
     3781 aagataaaat cagtgcaatt gctacaaaaa tttatggggc agaaggtgtt gagttttctg
     3841 taaaagctaa atctcaatta caacaaatta aaagtttcgg ttttgataat tttccaattt
     3901 gtatggcaaa aacacccaaa agtttctctg atgatgaatc taaacgaggt cgtccaactg
     3961 gttttacggt gaaagttcgt gaatttgaaa tcgcgtccgg tgccggattc attataccaa
     4021 ttttaggaga aatgatgcga atgcccggat taccgacaat tcctaacgca gaaaatatta
     4081 aaattgacga tcaaggagtg atttcaggat taagctaatt agcataattg tgtaagttaa
     4141 tagcttttca attgcttacc ttcggaaaac aattgaagca ttatgaaaag agtaggtttt
     4201 ttatttattg tactgatata tactcataat atacttggac aaggattctt tgaagggagt
     4261 gatattattt ctaaaacgta ttctcaacgt tgggaattgg attcaattga taaaaaagga
     4321 acttttagat tggtttctta taagtcaatt tatgtagctc cggctcgatg gtcaagcaat
     4381 ccaaatgaaa aaccgcaaag tgaaaaccct aataattctg caacggaaac aattgattat
     4441 gatattgtgg aagtgaaatt tcagttaagt ttaaaaacca aattattgca atccattttt
     4501 tggggtaaag gtgatctttg ggttggttat acacaaattg ctcattggca gatttataat
     4561 gaagaacttt cgagaccttt tcgggaaata aattatgaac cggaaatcat ctttaaatat
     4621 ccattgcaca tcaaggcatt taccggtgaa tttaagtcaa taggattttc atttactcat
     4681 caatctaatg ggagggagtt accttattca cggagttgga atcgtatcat ttttgatctt
     4741 tcatacgaaa ttgaaagttg gatagtatct gttagaccat ggattcgttt aagcgatggt
     4801 aaaaaagatg ataatcctgc aataactgat tatattggag atggtgaact taatttgtct
     4861 tatagctaca atcgtcatca attctatacc gtcatcactc atccattcaa tcaatttcaa
     4921 ggcggaagtg tacaactaaa ttatattttt ccgataaaag gtcatgttag aggacatttg
     4981 caagcttttc aaggatatgg tgaaacttta attgattata ataataaaca aacaacaatt
     5041 ggaattggag tttcatttgc aaattggtaa aattaaattt ttacgtttca aaaccgaaat
     5101 cgatatagtt aataaattac ttgtttaaaa ggtataattg tctaatttta tacaagaaac
     5161 ctgattttta tcagattttc acgtttcata tcggaataac tttgttgtat aattaaaagg
     5221 acagactatg aacttatctc acatcgaaca tttaggaatt gccgtaaaaa gtttagaaga
     5281 agccattccc ttttgggaaa acatgctcgg actaaaatgc tatgctattg aagaagtaaa
     5341 agagcaaaag gtaaaaaccg ctttctttca aatcggacaa tcaaaaatcg aattattaga
     5401 atctactgac cctgatggac caattggtaa atttattgag aaaaaagggg agggcattca
     5461 tcacttagcc atcgcggtta aagggattga agaaaattta gcagaacttg atcaaaaagg
     5521 ggtacaactc attgataaaa ctccacgaaa aggagctgaa ggtttagata ttgcttttat
     5581 acatccaaag tcgacacatg gagtattact tgaattgtgt gaaaataaaa acttgtaatt
     5641 cattttctta tggaaagtac agataaaatt aaattattat tagataaacg aattgaagcc
     5701 aaattaggtg gaggtcaaaa acgtattgat actcaacatg ccaaaggtaa acttaccgct
     5761 cgagaacgta ttgaactgct tttagacgaa ggtagttttg aagagtttga tatgtttgta
     5821 acgcatcgtt gtaccaattt tggacttgaa aatcaaaaat acttgtctga tggagttgta
     5881 actggttttg gtacaattga ttcgagatta gtttatgtat tctctcaaga ctttactgtt
     5941 tttggtggtt cattatctga aacttttgcc aataaaattt gtaaagtcat ggatatggct
     6001 cttaaaatgg gagcacccat tataggaatt aatgatagtg gaggagcaag aatccaagaa
     6061 ggtgtaacca gtttggccgg ttatgcagaa gtgtttcaac gaaacatcat ggcttccgga
     6121 gttattccgc aaatttctgc tgtttttgga ccttgtgccg gcggtgcggt atattcaccc
     6181 gctttaaccg actttattat gatgacaaaa caaaacagtt acatgtttgt tacggggcct
     6241 aaagtagtga aaacagtaac cggtgaagta gtaaccgacg aagaattagg aggtgccatg
     6301 gttcacggta cgaaatcggg ggttacccat tttgttgcgg aagatgagca ggagggatta
     6361 ttgttaataa gaaaattgtt gagctattta cctcaaaaca acttggaaga cccaccatta
     6421 tcagtatgtg ctgacccaat cgatcgttta atagatgaat tgaatacaat tataccagaa
     6481 aatccaaata aaccgtatga tgtaaaagat gttattcacg ccatcgctga tgaaaatgaa
     6541 ttttttgaag tacatagaca ttatgctcct aatattgttg taggatatgc taaatttaac
     6601 ggcatgccgg taggaattgt agccaatcaa cctagttatt tagccggtgt gttggataat
     6661 aatgcctctc gaaaagcggc tcgatttgtt cgattctgtg atgcttttaa tattccgttg
     6721 gtcaccttag ttgatgtacc tggattttta cctggaacaa ctcaggaata tggtggaatt
     6781 attattcatg gagcaaaatt attatatgct tatggcgaag ctactgttcc aaaagtgact
     6841 gtgattcttc gtaaagctta tggaggagca tacgatgtta tgagttcaaa acatttacgg
     6901 ggtgatatta actacgcttg gccatcagca gaaattgctg ttatgggacc aaaaggagct
     6961 atagaaattt tataccaaaa agagtacatg caaattgaag accttgagga aagagccaaa
     7021 tttatcgcgg aaaaagaagt agaatacaaa gaaaaattcg caaatccata caatgcagcc
     7081 agttatggtt acattgatga tgtgattgag ccaagaaaca cacgtttcag aattattcgt
     7141 gccttacaat cgcttgctac aaaaaaagtg gttaatccac caaaaaaaca ttctaatctc
     7201 ccattgtaag atgaatacgt tattgctcgt acaacaaccg gtgagtcata aagcggccgc
     7261 tgaatttgca aagttagatc cctatgggat tggaatgaca atggtagcta tggctgtagt
     7321 ctttctatcg ctaataatac tatatgtcac attcaagaat attgccaaac tttttgccta
     7381 tgatttcaaa gctaaaccgc aaataaaagt agagggaatt gaactttcaa ataccaaaag
     7441 ccagcaaact ttagagttag gagctgccat agcaatggcc ttgcatttgt atcaaaatga
     7501 attacatgat cacgagaatg cattgctgac cataaaaaaa gtaaacggta attattctcc
     7561 atggagttca aaaatttatg gaatgcgaaa agaactaaaa taatcacaca tgaaaagata
     7621 taaatttaaa ataaacggaa acagttacga tgtaaaaatc gacagtattg aagaaaatca
     7681 agccattgta gacgtaaacg gagtttcctt tgaagtggaa ttagaaaaga aatcatctat
     7741 tgcaaaaacg cctaaattgg ttcgttctgt agcagtgccc tcaactgatt cgtcacaagc
     7801 tgttgcaaaa accagtagtc catcagctcc aaaaggagga ggaaccatta aatcaccact
     7861 tccgggtgtg attttaaaag tacatgtaaa agtaggtgac gttgttaatt tgtcacaaga
     7921 gttaattacg cttgaagcca tgaaaatgga aaatagtatt caggctgata aagcaggagt
     7981 agtgcaagca atacacttta atacaggaga atctgtaatg gaaggtgatg ttttattaac
     8041 aataggatca taaatcatgg ataatttctg gagtttttta gccgagcatt tgcatcagtt
     8101 tttcacatat acggcttttg caaattttac cattggtcat ggtgtgatga ttttaattgg
     8161 tctgattttt atttatttag ccatttttaa agaatatgaa cccatgttat tggtacccat
     8221 tggttttgga atgttaattg gaaacattcc tttttatcct gggctacaaa ttggaattta
     8281 tgaacaaggc agtgttttga attacctcta ttttggggta atgaaaggta tctatccgcc
     8341 attaatattc ttaggtattg gagcaatgac cgatttttca gcgttgattt ccaatccgaa
     8401 actgatgcta attggtgcag cagcacaatt gggaattttt ggtgcatata tggcagcatt
     8461 ggctttaggt ttcttacctg aacaggcggg agcaatagga atcattggtg gtgctgatgg
     8521 acctactgct atattccttt cgtcaaagtt ggctcctgaa ttaatgggag caattgcgat
     8581 ttctgcgtat tcctacatgg cattagtacc tgtgatacag ccaccaatta tgcgattgtt
     8641 aacaactagt aaagaaagat taattcgtat gaaaccgcca agagttgttt cacgaaatga
     8701 aaaaattatg tttcctattt ttggtttgtt gttaactaca tttattgtac ctagtggttt
     8761 acctttattg ggaatgttat tttttggtaa tttattaaaa gaaagtggtg taacaaaacg
     8821 tttagcggat actgctaagg gttcattgat tgatatagtt actattctaa ttggtttaac
     8881 agttggtgct tcaacacaag ctactacttt tttaaccgga caatctgttg gtatttttgc
     8941 cattggagca gtatcattta tcattgcaac ttttgggggc gtaatgtttt gtaaaatatt
     9001 taatctattt ttaaaagagg gtaataaaat taatccttta atagggaatg caggagtttc
     9061 tgcggttccg gatagtgcaa gggtttcaca agttattggt ttggaatatg atagaactaa
     9121 tcacttattg atgcatgcta tgggacccaa tgtagccgga gttattggta gtgctattgc
     9181 agcaggggta ttgttaagct ttttgggtta agtagatttt ggaatcttaa aatcgttaag
     9241 gtaattatct aacaattcac cggctttgaa aactttttta aatagtattt cttcggtgcc
     9301 gtaatgaaaa tgcacttcaa cgaagaaagt atgtaaactg tataattttt taatcaccgg
     9361 cttttcaagt cgctcggcca ctaccgctcc tttttcaaag aggatgcggt attgttcgtt
     9421 ttcaggaagt gctaaaaagg aaattagttt caatgatttg attttaataa acgaagatac
     9481 caaaaaacaa ctaggtagta agcataaaaa aaccgattaa atttaaagaa ttctttaaat
     9541 ttaatcggtt cgttttatta aacaactttt actttacacc agtaaatgtt cccattgaca
     9601 tttgtgtttt atcagtagtc gttattttag cgaaaagaaa tttttgggtg tcggggaaat
     9661 aatgaaccaa ttggtttaca taggcaacgc ttctctctaa attattattg tcataataac
     9721 caaagctaat agaacgagca attttttcat tattgaaaag ataattccat ttgaaaaaac
     9781 cattttctaa tccggtttga acaaacaaat caccatttgc attaactaat ccaaaagaat
     9841 ggtataaatc agcttcaccg ctagtgctta ccggaattgg tttgattgat tttaaaatac
     9901 cttcaaaatt taaagtgatt aagtaagatg gaccaaatgt gtgtttttcc accggaaaac
     9961 ctgttgagcc gggagttggt tttggtgtgg catcaacttt cgcttcagca aaaatggcaa
    10021 tttcattgtt ttgcatcaaa atagtgcgaa agtttacctc ttcaatttta gtaatcgaat
    10081 taaaaccatc aattttatta ttttgtaaaa tagcaccgga acttaaatca tataacatta
    10141 aatcaccaaa gtcaccgcga cgtactcctt tagagggata attcattgca agcaagtaat
    10201 cctgtgttcc aattgaaata gcaataggct catgaatttt gatactttca cccactttta
    10261 aatcggtttg ttctgtttca gaaacagcca ctaaataatt gtctaattta taaccgtagg
    10321 gttggcgaac taatactact tttccggaat tggtagcgac taagttacct gtaaagaact
    10381 cattagcgaa agttacttct tttttccaag caacatccaa tgtagtgctg ttaagtacca
    10441 ttaaattgtt tacttcaggt tctttcttgg aattagcagc tttgtagtta actacaaaaa
    10501 aagcaccatt ctgtgattgt tttaccttaa catcacctga tttagagttg gatacaattg
    10561 gaaatcccat aagcatcgta ctggtgaatt tcttagatgt tttgtcaaaa acgtgttgat
    10621 atacttcgtc tctttttgat tttcctgaat aactttgtgt aaaaaccact gctttttcag
    10681 ttccaatagc gtacgagcct aaaaaattat gcaaagttcc gccaccttct aatactggaa
    10741 aaggatgtgt tatagtttcc attaaagcat tcttttgatc aaattttcga aggataattt
    10801 gattactcga catcatcccg tatttattca ctacggttaa taagtaataa ttgtaattgt
    10861 cggtcaaaac gaattttggt tctaattcat tttttgggtc aaaatcaaat ttttctcccc
    10921 attcaggtgt ttgagcaaaa gtgtttccaa aaacacaaag aaaaaggaac gcgattattc
    10981 ttaatttcat gttgatttat tttttataga tttcgttttc caacgctttt aattcgtttt
    11041 gttcatcgta gctaagttta accgatatta ggttttcttg taattcgttt aaatattttt
    11101 cagcttcaga ttttttgttc attgctaaat tcaatcgaat taaattgaag taaatgtatt
    11161 caccaatttt tgcattaaaa tcagattttg aatctttttt aaattctact ttttttaatg
    11221 tttctaacca aatgtcaatt cctttttgca tagcagttac agcagcagca cttcgttccg
    11281 gttcagaagg ttgtaatttt tttaaattgg ttgttacata aatatgtgct ttttctaaat
    11341 catcatattg ccctttgttt tttactttat ccaattttac tgttttttta atagattgga
    11401 atccgtaatt atcatttaaa tattggttta aaaaagcgac aactttattt aaatgatttt
    11461 tttcttctaa tggtttgttg atgttattgc taggtgaaga agaaacaaat ttgaattctt
    11521 gaaaaaactt agtatttatt ttttcaactc cgtttacttt taccactaac gttgtaggtt
    11581 gattggcaaa ggtttgtccg gcattatctt gaaattgtac gccacgaata tctacagcaa
    11641 catctaaata atttccttct ttgctaaatc cttccacatt gatttgtgaa gacaatacat
    11701 tgttgtccac aatgatgtaa ttgtttaaat taggctcttg ataaacaggt gggagcggtt
    11761 taacataaac aggttttgtt ggatttacta atcgtgggtt tttcccttgg tctgtcattg
    11821 acatacgttc caataaagaa agttttttaa attctgcaga ttctaattca aatttttctt
    11881 ttgctttgat aacttcttga tcgtaatctt ttagtttttg ttgaaattct ttttcgctat
    11941 tagcgacaac agttgtgtaa ttatctaaag cagcttgatg atccactttt gattgtttga
    12001 taacatcatc tgcggtaaca ttataaggag aagttaccgt aacttttaac aatctatttg
    12061 cgacgtcaat cgtttctttg ggttgcttta aaataagaaa atcaatgttt tctgatgaaa
    12121 tgttttgtgc ataaatagaa atagtactaa caaaaacaaa taacgaagcg aaaaaggtat
    12181 ttaatttcat tgaaaaaggg tttttataat gagttgcaaa attagtatta ttttctataa
    12241 aactcctaaa agcttcttaa ataagtggat aaccaattaa tttatttaag gagcagacaa
    12301 aaaaaagatg aaccgttaaa agttcatctt ttttgtattt agtcaatgtg tttttaaaat
    12361 aacatttttg ataatacatt actactagta ttgctttttg gaatgagtgc tgttaacaat
    12421 ttcatttggt cagcagaaat taatttcttc attttttccg gaaataaatt gcggattgaa
    12481 gtcatttttg catcataatc ctttttgttg gtggctgctg tgggtaaaat gtctttcttt
    12541 ttgtttaata agtcatttac taatgaagta actgc
//
LOCUS       DAOTCW010000062        12391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3173_length_12391_cov_42.773022, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000062 DAOTCW010000000
VERSION     DAOTCW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12391
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3173_length_12391_cov_42.773022"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="P5335_06605"
     CDS             complement(<1..233)
                     /locus_tag="P5335_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74582.1"
                     /translation="MKNIFTQLNNLKVNQISNTIFPKIGLFCLLLLVNLTQAQTVLID
                     PSGEGGFENGTSFAANGWTATIGTSTQNQWVCNT"
     gene            526..2454
                     /locus_tag="P5335_06610"
     CDS             526..2454
                     /locus_tag="P5335_06610"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS sorting signal type C domain-containing
                     protein"
                     /protein_id="HRZ74583.1"
                     /translation="MRKLLNFLPLLTCFFSVLISHAQLHVSSGSFVYVQNQYVTVTQD
                     VNLAANGDIYLRDESQLLQKTSGVSANTGLGSLSVFQEGTSNNFGYNYWCSPIGVPSA
                     SPLNSNFGITLLKRPDGVSPISFSPGGQTTTTSNNGTTTNSLLTISSRWIYTLTTANQ
                     YSSWNFIGAGTTIAAGQGFTMKGVSGTDNTSVLGVQNNPTNNQRYDFRGKPNDGTIGI
                     PVVSDPGPNYTNSTLTGNPYPSAINLNLFLLENSGHTINYGLGTATFVGSPIIDGAAY
                     FWEHEKPGTTHVLANYVGGYSSYVANNGPFSPGTYANEPWTTYNSDGTPNTSSTGFGV
                     NYYRMFSPVGQGFIIKGVVPTPSPDAQMKNDYRVFVKEGGMTTNSEFERNASNENSTD
                     TNENWPAIPNLTGTDYTQFSKAQIPQIKIHTILDNQFTREIVLAFNPNSNDGYDFAFD
                     ANTQELNLPTDAFFPIVDNKQFVISTLPFEITKRIPFAVKASSESNFRVLVGNLINFD
                     MAENIYLYDKQTTIYHDIKNGFVDFTLPAGNVTDRFEITFLNETLSDPDNTNYNFDVI
                     HNNENKKLSISNPNLLNVKSVSLFDITGKLIFNKEQLAAEPSYEFSTSGLSDSVYIVK
                     INTAENKEISKKVLIFKE"
     gene            2542..3450
                     /locus_tag="P5335_06615"
     CDS             2542..3450
                     /locus_tag="P5335_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRZ74584.1"
                     /translation="MEFKVINQEVIGFNKNVSLFIKREDLIHPYISGNKYRKLKYNLE
                     QAKAENQDTLLTFGGAFSNHIAAVAFAGKEYGFKTIGVIRGDELESKIKDNPTLLFAQ
                     QCGMKFIFETREKYRHKNEATYQSSLIAQFGQFFLIPEGGTNELAIKGCEEILTQEDA
                     VFDYICCCVGTGGTISGIINSSLNYQKVIGFPALKGDFLADEIRKNVNNNRWELNQDY
                     HFGGYAKTNNALIQFMNGFLENTKIQLDPVYTGKMLFGLYDLVEKGFFPCSSRILAIH
                     TGGLQGIQGMNIKLEQLHLPKIKIHV"
     gene            3443..4288
                     /locus_tag="P5335_06620"
     CDS             3443..4288
                     /locus_tag="P5335_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRZ74585.1"
                     /translation="MFKKVIFLLSLLIITACSSSKPTIRTSKPSTPITRKVKKPVQKQ
                     TSNSTVKNENENTPEVLEATSKVKVTPEMVIKYIEDFKETAKNNMRQFGVPASITLAQ
                     GILESGVGTGTLSQKANNHFGIKCHKEWTGPSVLFDDDSEKECFRKYKDPAESYRDHS
                     LFLTSRSRYDSLFELPQGDYKAWAKGLKNAGYATDPKYPDKLISLIERYNLHQYDVEV
                     VGKSAIQTKSNSEIITTKEEYVVVKGDTLYSLSKKFNISVDELKKKNNIKSDSLSVGQ
                     KISVK"
     gene            4314..5600
                     /gene="hemL"
                     /locus_tag="P5335_06625"
     CDS             4314..5600
                     /gene="hemL"
                     /locus_tag="P5335_06625"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRZ74586.1"
                     /translation="MLYQRSSELFAEAEKVIPGGVNSPVRAFKGVGGTPIFVKSAKGA
                     YLYDEDGNRLIDYINSWGPMILGHAHEPVITAITERAKLGTSFGMPTELETQIAALAV
                     SMVPNIDKIRFVNSGTEACMSAVRLARGFTKRDKIIKFSGCYHGHSDSFLIQAGSGAS
                     TFGIPNSPGVTQGTAKDTLLANYNDLQSVAQLFESNKDEIAAIIVEPVAGNMGCVPPL
                     NGFLEGLRKLCDANGALLIFDEVMTGFRLAKGGVQELFGIKADIVCFGKVIGGGLPVG
                     AFAARNEIMNYLSPIGPVYQAGTLSGNPLAMAAGFAMLNELNQDDAIFKRLDEKTTYL
                     ETGIRDVLTKNQIAFTINKVGSMISVHFNETPVIDFQTAKGGDNETFKKFFHGLLKEG
                     IYIAPSAYETWFITDALTYEDLDFTIKAIDTVSKQF"
     gene            complement(5653..6018)
                     /locus_tag="P5335_06630"
     CDS             complement(5653..6018)
                     /locus_tag="P5335_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74587.1"
                     /translation="MKKIVAIVLFMIAFGFSSNAQSSISNAKPTSREVNPKEAAYKDI
                     SDLSAVIDITPEFKNDLMTLFMMREEAIAGCTTEDERVITYQKFGEKIMSGLTEEQRK
                     TVAQKPELYARLTKYKTAK"
     gene            complement(6096..6563)
                     /locus_tag="P5335_06635"
     CDS             complement(6096..6563)
                     /locus_tag="P5335_06635"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRZ74588.1"
                     /translation="MKNVIVTAILLFGLITFAQEGQINASNGRPARMGQKQNFTPEQR
                     AELQVKRMTLHLDLNQKQQVEVKKLVLEQAKKRETIQTEMRAKRESGVKPTDEERFAN
                     QSQMLDEKIAMKTAFKKILTPEQMAKFEENLSNRSEKMQARRMNKSKKFANKK"
     gene            6711..6971
                     /locus_tag="P5335_06640"
     CDS             6711..6971
                     /locus_tag="P5335_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74589.1"
                     /translation="MEEIQNANQKPMSVKDWLVTLLIMAIPLVGFIMLFVYAFGNNEN
                     VNKQNWAKAQLIVLAIVIGLMIVFISVFGAIFASAMAGSQNY"
     gene            complement(7028..8719)
                     /gene="lysS"
                     /locus_tag="P5335_06645"
     CDS             complement(7028..8719)
                     /gene="lysS"
                     /locus_tag="P5335_06645"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRZ74590.1"
                     /translation="MQLSEQEIIRREKLNILRGMGINPYPADLYPVNHTSTEVKENYE
                     EGKKVIVAGRIMSTRIQGKASFASLQDSEGRIQLYFNRDEICPGEDKTLYNEVFKKLI
                     DLGDFVGVEGELFITQVGEKTIKVLDFKLLSKTLRPLPLPKTDTEGHVFDAFTDPELR
                     FRMRYVDLVVNPHVKEVFVKRTKLFNAMRTFFNDKGYFEVETPILQSIPGGAAARPFM
                     THHNSLDIPLYMRIANELYLKRLIVGGFDGVYEFSKNFRNEGMDRTHNPEFTAMEIYV
                     AYKDYNWMMDFTENLLEFCAIHVNGSTNATFGEHQVNFKAPYTRITMTDAIIQFTGFD
                     ISGKTEDEIRIAALNMGVQVDDSMGKGKLIDEIFGAKCEGSFIQPTFITDYPKEMSPL
                     CKEHRTNPELTERFELIVCGKEVANAYSELNDPIDQRERFEAQLNLADRGDDEAMFID
                     NDFLRALEYGMPPTSGLGIGMDRLVMFLTNNPSIQEVLFFPQMRPEKKQVELSEDEKI
                     VVEILKISNHISLSELKEKSALSGKKWDAAMKGLAKNNLVKVHVEGELKTVELKG"
     gene            8931..9773
                     /locus_tag="P5335_06650"
     CDS             8931..9773
                     /locus_tag="P5335_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74591.1"
                     /translation="MNLKSKIAYVLLLFSLSSCVITESLLINEDNSGTITYEMDGSSM
                     MTMLEEKMGKPEDTSKKRKKKSKKEKDASSAKVIDSTFSLKELYAEKQDSIAKLSKEE
                     QEQIKKMENFKIRMIIDEENKIMKYILFADFKSMDELQNMTSPINTFKSIGAGGSEEL
                     TKNNQLTNDALTTFSYDGKIFKKTVQKSNFKEDFKEALKKENSTDEDIDVEEIAEGVD
                     ESMNMIFEQSSYTIIYQFPKPIKSVSVENAVISDDRKTVTIPYQMKDYMSNPESLNFE
                     INFE"
     gene            9775..10503
                     /gene="lipB"
                     /locus_tag="P5335_06655"
     CDS             9775..10503
                     /gene="lipB"
                     /locus_tag="P5335_06655"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRZ74592.1"
                     /translation="MNKKIQLQDLGSKDYKLTWDYQEELFKKIVDIKILNRREGSEIE
                     TPNYFLFVEHPHVYTLGKSGDISNLLLSEKQLEAKKATFYKINRGGDITYHGPGQIVG
                     YPIIDLENYFTDIHKYLRFLEEVIILTLAEYNIIGKRSEGETGVWLDVGTPFARKICA
                     MGVRASRWVTMHGFALNVNADLGYFDNIIPCGIRGKAVTSLQVELGVEKVEEDQVKEK
                     IVKHFSNLFEAEFINPISIDQVII"
     gene            complement(10466..11158)
                     /locus_tag="P5335_06660"
     CDS             complement(10466..11158)
                     /locus_tag="P5335_06660"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRZ74593.1"
                     /translation="MLKFRYSEYEFECGTDEAGRGCLAGPVTAAAVIGPLRFGQENEA
                     NLIEKYFELLNDSKQLSEKVRFELRPFIEETAFSFAVTHIYNVEIDEINILNASMKAM
                     QESILKLKTIPNYIIVDGNRPLNGKLGMIQKNGKIFTPKETELLKSIPSTSIIKGDSK
                     YVSIAAASVLAKTYRDEFMNLIHEEFPMYNWKQNKGYPTKEHREAIAKYGTTKYHRMT
                     FRLLPDQLKLDL"
     gene            11355..>12391
                     /locus_tag="P5335_06665"
     CDS             11355..>12391
                     /locus_tag="P5335_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRZ74594.1"
                     /translation="MRSKFKWIFTLLLAFSMQFSFAQEKTVSGTVTDASGMPLPGATV
                     VVKGTSRGTSTDFDGKYSIKASVGETLDFSFVGYKAVSVKVAASNVVNATLKDDTALD
                     EVVVTGAFGVKKKEKAVTYAAQSIKGEAMTEAREANLVNALSGKVAGVQVTSSSGSVG
                     ASARIVLRGASSITGNNQPLFVIDGVPFDNTNYGNADSSGGRDLPNGAASINPDDIES
                     INVLKGPTAAALYGLRASNGVILITTKSGKNKGKFEVSFNSNITFSDPLKLPNYQNSY
                     GQGATSDFFQFVDGSGGGYNDGVDESWGAPLDVGLNFVQWDSYKVGGAPLPWVSHPDN
                     VKDFYDTGVSIT"
BASE COUNT         4157 a         1922 c         2148 g         4164 t
ORIGIN      
        1 gtattgcaaa cccattgatt ttgagttgaa gtgcctatag tagctgtcca accatttgct
       61 gcaaatgaag ttccattttc aaatccaccc tcgcctgatg gatcgataag cacagtttga
      121 gcttgggtta aatttactaa taacagtaaa caaaacaatc ctatttttgg gaaaattgta
      181 ttgcttattt gattgacttt taagttattc aattgagtaa agatattttt cataacctat
      241 aatttttaaa ttcgatttca gttcatgatt tttgacttga caaatatata caaaaaccga
      301 taaagtgtat taaaaaatcg atgaaatgca taaataaata aattatgtaa gaaaaaagat
      361 gttatattta gcgttaaaat ataaaattta tataatttct ttagttaatt gagtttttgt
      421 ttttaaagtc gttaaagcct taatatgttt attttatgaa atccttgttt ataaagacaa
      481 aaatttgatt aattatttat atatttgaac tttaagtttt tagatatgag aaaattactg
      541 aactttttac cccttctaac gtgttttttt tccgtgctca taagtcatgc tcaattgcat
      601 gtgagttcag gaagtttcgt ttacgtccaa aatcaatatg taaccgttac tcaagatgta
      661 aatttagcgg ctaatggaga tatctactta cgtgatgaaa gtcagttgtt acaaaaaaca
      721 agtggcgtct cagcaaatac agggttaggc tcactttcag tttttcaaga aggaacctcc
      781 aataattttg gctacaatta ctggtgttca cccattggag taccttctgc aagtcctttg
      841 aattctaatt ttgggattac attattaaaa agaccggatg gagtatctcc gatatctttc
      901 tcacctggtg gtcaaacaac tacaacttct aataatggta ctacaactaa tagcctttta
      961 actatttcaa gccgttggat ttatacatta actacggcta atcaatactc tagttggaat
     1021 tttattggtg cgggaactac cattgctgcc ggtcaagggt ttaccatgaa aggtgttagc
     1081 ggaacggaca acacatcagt acttggtgtt caaaacaacc caactaataa ccaacgctat
     1141 gattttagag gaaaaccaaa tgatggaaca ataggaattc cagttgttag tgatcctgga
     1201 cctaactata caaatagtac attaaccgga aatccatatc cctcagctat caatctaaat
     1261 ttatttttat tggaaaatag tgggcatact ataaattatg gcttaggtac agcaaccttt
     1321 gtaggttctc ccattattga tggagcagct tatttttggg aacatgaaaa accgggaaca
     1381 acccacgttt tagcaaatta cgtcggaggc tatagcagtt atgttgccaa taatggccct
     1441 ttttcaccgg ggacttatgc taatgaacct tggactactt ataatagtga tggtacacca
     1501 aatacatcaa gtactggatt cggagttaat tattatcgaa tgttttctcc agttgggcaa
     1561 ggatttataa ttaaaggtgt tgttccaact ccttctccag atgctcaaat gaaaaatgat
     1621 tatcgtgttt ttgtaaaaga aggaggtatg actactaatt ccgaatttga aagaaatgca
     1681 agcaatgaaa actcaacaga taccaatgaa aactggccag ctattcctaa tttaaccgga
     1741 acagattata cgcagttttc caaagctcaa attcctcaga taaaaattca tactatttta
     1801 gataatcaat ttacaagaga aatagtactt gcatttaatc caaattctaa tgatgggtat
     1861 gattttgctt ttgatgccaa tacacaggaa cttaatttgc ctaccgatgc tttttttcca
     1921 attgttgata ataaacaatt tgtgatttca acattacctt tcgaaattac taaacgaatt
     1981 ccttttgccg ttaaagcttc ttctgaatca aattttagag tgttagtagg taatttaatc
     2041 aattttgata tggctgaaaa tatttatctc tatgataaac aaacaactat ttatcatgat
     2101 atcaagaatg gtttcgtaga ttttacattg cctgccggga atgtaactga ccgttttgaa
     2161 attacttttc taaacgaaac attaagtgat cctgacaata ccaattataa ttttgatgtg
     2221 attcataaca atgaaaacaa aaaattgtct atcagtaatc caaatttatt aaatgtgaaa
     2281 tctgtatcat tatttgacat aacaggaaaa ttaatattta ataaagagca attagctgct
     2341 gaaccatcct atgaattttc aacttccgga ttaagtgaca gtgtctacat tgttaagatt
     2401 aatactgctg aaaataagga aatcagtaaa aaagtattaa tttttaaaga ataattttaa
     2461 tcgaaagaca aatttatcca aaagacagtc attatggctg tctttttttg tttaagtcaa
     2521 attcagtact tttgttggcg tatggaattc aaagttatta atcaggaagt gattggattt
     2581 aataagaatg tctccctttt tattaaaaga gaagatttaa ttcatcctta tatttccgga
     2641 aataaataca gaaaattaaa atacaacttg gagcaagcga aagccgaaaa tcaagatact
     2701 ttattaactt ttggtggagc tttttcaaat catattgctg cagtagcctt cgcaggaaaa
     2761 gaatatggtt ttaaaacaat tggagtaatc cgaggtgatg aattagaatc aaaaataaaa
     2821 gataatccaa ccttattgtt tgctcagcag tgtggcatga aatttatatt tgaaacgaga
     2881 gaaaaataca gacacaaaaa cgaagcaact tatcagtcta gtttaattgc tcaatttggt
     2941 cagttttttc taattccgga aggtggaaca aatgaattag ccatcaaagg atgtgaagaa
     3001 attttaacac aggaagatgc cgtctttgac tatatttgct gttgtgtagg aaccggaggg
     3061 acaatttcag gtattattaa ttcttcgttg aattatcaaa aagtgattgg ttttccggct
     3121 ttgaagggtg attttctagc tgatgaaatt cgtaaaaatg tcaacaataa tcgttgggaa
     3181 ttaaatcagg attatcattt tggagggtat gctaaaacaa acaacgcgtt gattcaattt
     3241 atgaatggat ttttagaaaa cactaaaata caattagatc ccgtttatac cggaaagatg
     3301 ttgtttggat tatatgattt agttgaaaag ggattttttc cctgtagttc aagaatcctt
     3361 gctattcaca ccggaggact tcagggtatt caaggtatga atattaaatt agaacaatta
     3421 catttgccca aaataaaaat acatgtttaa gaaagtaatt ttccttttaa gtttattaat
     3481 aataacggca tgttcttcgt ctaaaccaac gattagaact tcaaaacctt caactccaat
     3541 tacaagaaaa gttaagaagc cagtacagaa acaaacttct aattccactg taaagaatga
     3601 aaatgaaaac actcctgagg ttttagaagc tacttcaaag gtaaaagtta cgccggaaat
     3661 ggtgataaaa tatatcgaag attttaagga aaccgccaaa aataacatga gacaatttgg
     3721 agttccggca agcattactt tagcacaagg aattttagaa tcaggagttg gaacaggaac
     3781 tttaagccaa aaggcaaata atcattttgg cattaaatgt cataaagagt ggacaggacc
     3841 tagtgtgctt ttcgatgacg attctgaaaa agaatgtttt agaaaataca aagatccggc
     3901 tgagtcctat cgtgaccatt ccttattttt aacttccaga agccgttacg attccctatt
     3961 tgaattacct caaggtgatt ataaggcatg ggcaaaaggg cttaaaaatg ccggttatgc
     4021 aacagaccca aaatatcctg ataagttaat cagtttgata gagcggtata atttgcatca
     4081 atatgatgtt gaagttgttg gaaaatcagc tattcaaaca aaatcaaatt ccgaaataat
     4141 aactaccaaa gaagaatatg ttgtggttaa aggagatact ttgtattcac tttccaaaaa
     4201 atttaatatt tctgttgacg aattaaaaaa gaaaaataat ataaaatcag attcgctttc
     4261 tgttggacag aaaatcagtg tgaaataatg attaaaaaaa taaataaaat acaatgttat
     4321 accaaagaag tagtgaactg tttgctgaag ctgaaaaagt aattccggga ggtgttaatt
     4381 ctcctgtacg agcttttaaa ggagttggcg gtacaccaat ttttgtaaaa agtgctaaag
     4441 gggcttattt atacgacgaa gatggaaaca gactgattga ttatatcaac tcttgggggc
     4501 caatgatttt aggtcatgct catgagcccg tgattactgc aataactgaa cgagctaaac
     4561 ttggaacttc tttcggaatg ccaacggaat tggaaacaca aattgccgca ttagctgttt
     4621 cgatggtacc aaatattgat aaaattcgtt ttgtaaattc gggaacagag gcttgtatga
     4681 gtgctgttcg tttagctagg ggatttacaa aaagagataa aattatcaag ttttcaggct
     4741 gttatcatgg acattcagat tcctttttga ttcaagcggg tagtggtgcc agtacatttg
     4801 gaatacccaa tagtcccggc gtaacacaag gtacagcgaa agatacttta ttggccaatt
     4861 ataatgattt gcaaagtgta gctcaactct ttgagtcaaa taaagatgaa attgcagcaa
     4921 tcattgtaga acccgttgca gggaacatgg gctgtgtgcc acctttgaat ggatttttag
     4981 aaggtttaag aaaactttgt gatgctaatg gagcattact gatttttgat gaagtaatga
     5041 ccggatttcg attggcaaaa ggaggtgttc aagaattgtt tggaatcaaa gccgatattg
     5101 tttgcttcgg aaaagtaatt ggaggcggat taccggttgg tgcttttgct gcccgaaatg
     5161 aaattatgaa ttacttatct cctatagggc ctgtttatca agcgggaact ttatccggga
     5221 atccgttagc gatggcagcg ggttttgcca tgttaaacga attgaatcaa gatgatgcta
     5281 tttttaaacg tttggacgaa aaaacaacat atttagaaac tggaattcgg gatgtattga
     5341 ctaaaaacca aattgctttt acaattaaca aagtcggttc catgatttct gtccatttta
     5401 atgaaacgcc ggttattgat tttcaaacgg ctaaaggggg agataatgaa acctttaaaa
     5461 aattctttca cggattatta aaagaaggca tttatatagc tccatcagcc tatgaaacat
     5521 ggtttattac agatgcttta acttatgagg atttagattt tacgattaaa gcaatcgata
     5581 ctgtttcaaa acaattttaa ataaaaaaat cccgattact cgggattttt tgtgcttttc
     5641 atataactgc tgttattttg ctgttttgta tttcgttaac cttgcatata attctggttt
     5701 ttgagcaaca gtttttcttt gttcttcagt taaaccggac attattttct caccgaattt
     5761 ttggtatgta attactcttt cgtcttcagt agtacaacct gctattgctt cttcacgcat
     5821 cataaacaaa gtcatcaaat catttttaaa ttccggagtg atgtctataa cagcactcaa
     5881 atcagaaata tctttgtaag cagcttcttt aggatttact tctcttgaag taggtttagc
     5941 attacttatt gaactttgtg cattagatga aaatccaaaa gcaatcataa acaatactat
     6001 agcaacaatt tttttcataa taacggtcat ttttagttag ttaatgagcg aatataaatc
     6061 aaattgacta actgacaaaa tattgtttaa aaaaattatt ttttgtttgc aaattttttc
     6121 gatttattca ttcttcgagc ttgcattttt tcagagcgat tagacaaatt ttcttcaaac
     6181 ttagccattt gttcaggagt aagaattttt ttaaaagctg ttttcatagc gattttttca
     6241 tctagcattt gactttgatt agcaaatcgt tcttcatcgg ttggttttac accactttct
     6301 cttttagctc tcatctcagt ttgtatggtt tctctttttt tggcttgttc caatactaat
     6361 ttttttactt ccacttgctg tttttgattt aaatctaaat ggagcgtcat tctttttact
     6421 tgaagttctg ctctttgttc aggtgtgaaa ttttgttttt gacccattct tgcaggtctt
     6481 ccattactag cattaatttg gccttcttgt gcaaatgtta ttaatccaaa taacaaaatt
     6541 gcggttacga ttacattttt cattttattt gttttttatg aatgatatac ataagaccat
     6601 taataatttg aatagtttaa tgcttaggat atttttaaca aaaaagacta cacaaaatgg
     6661 aattcttaca tttttaagta tattgcatgc ataaccaaaa aaactcacac atggaagaaa
     6721 ttcaaaatgc aaatcaaaaa ccaatgtcag tcaaagactg gctcgtaaca cttttaatta
     6781 tggcaattcc tttagtagga tttattatgt tatttgttta tgcctttggt aataatgaaa
     6841 acgtaaataa acaaaattgg gctaaagcac aattgatagt tttggcaatc gtaattggtt
     6901 taatgattgt ttttatttcc gtatttggag caatcttcgc ttctgcaatg gcgggatctc
     6961 aaaattatta aaaacaaaaa aatcccgatt acttatagtc gggatttgat tttgtaattt
     7021 atttaaatta acctttcaat tcaacggttt taagctctcc ttcaacatga acttttacca
     7081 aattgttttt ggctaaacct ttcatagccg catcccattt tttgccgctt aaagctgatt
     7141 tttcctttaa ttcagatagt gaaatatggt ttgagatttt taaaatttct actacaattt
     7201 tttcgtcttc ggataattcc acttgttttt tctccggacg catttgtggg aagaataaaa
     7261 cttcctgaat ggatggattg ttggttaaaa acataactaa acgatccatt ccaataccta
     7321 atccggatgt tgggggcatt ccatattcta atgctcttaa aaagtcgtta tcaataaaca
     7381 tggcttcgtc atcaccacga tcggctaaat ttaattgtgc ttcaaaacgt tctctctgat
     7441 caattgggtc atttaattca gaataggcat ttgccacttc ctttccacaa acaataagtt
     7501 caaatcgttc cgtaagttca ggattagttc gatgttcttt acataacgga ctcatctctt
     7561 taggatagtc ggtaataaat gtaggttgaa tgaagctacc ttcacatttt gctccaaaaa
     7621 tttcatcaat taattttcct tttcccatgg aatcatctac ttgaacgccc atgtttaaag
     7681 cagcaattct aatttcgtct tctgtttttc ctgaaatatc aaaaccggtg aactgaatga
     7741 ttgcatcggt catggtaata cgggtatagg gagctttaaa attcacttga tgctcaccaa
     7801 acgtcgcatt agttgaacca ttgacgtgta ttgcacaaaa ttctaaaaga ttttcagtaa
     7861 aatccatcat ccaattgtag tctttatagg ctacataaat ttccattgct gtaaattccg
     7921 gattatgcgt tctgtccata ccttcatttc tgaagttttt agaaaattca taaacaccgt
     7981 caaaacctcc aacaattaat cttttcaaat ataattcatt ggcaatccgc atatacaatg
     8041 gaatgtctaa actattatgg tgagtcataa atggtctcgc agctgcacca cctggaatag
     8101 attgtaaaat tggtgtttct acttcaaaat agcccttgtc attaaaaaat gttcgcatgg
     8161 cattaaaaag ttttgttctt ttaacaaaga cttctttcac atgtgggttt acaactaaat
     8221 cgacataacg catacggaaa cgcaactcag gatcggtaaa tgcatcaaaa acgtgtcctt
     8281 ccgtatctgt tttgggaaga ggtaaaggtc ttagtgtttt actcaaaagt ttaaaatcta
     8341 aaactttaat tgttttttca ccgacttgcg tgataaataa ttcaccttct acaccaacaa
     8401 aatcaccaag gtcaatcagt tttttaaata cttcattata taatgttttg tcttctcccg
     8461 ggcatatttc atctcgatta aaatacaatt gaattcttcc ttcactatct tgtaaagatg
     8521 caaaagaggc ttttccttga attcgggtac tcataattct tccggcaaca atcactttct
     8581 tgccttcttc atagttttcc ttcacttcag ttgaagtgtg attaaccgga taaagatcag
     8641 cagggtatgg gttaatcccc attcctctta aaatgtttag tttttcgcgt cgaattattt
     8701 cttgttccga aagttgcata gcatcaattt ttaaaggtgc aaagataatt tttttttagt
     8761 taaggacaac tagaatcaat aaactcaact atttttaatt agttagaatt tcttaagatt
     8821 tagaaagatt tttatggaca acttatttat gcttttttgg gaaagaggta gcggttatca
     8881 aaagccttaa acaataaact atttgtatat ttgtacttta atttaaagaa atgaatttaa
     8941 aaagcaaaat agcatacgta ttactattat ttagtttatc aagttgcgtc attacggaga
     9001 gtctacttat taatgaagat aactcaggaa ctattactta tgaaatggat ggctcttcga
     9061 tgatgaccat gctggaagaa aaaatgggca aaccagaaga tacttccaaa aaaagaaaaa
     9121 agaaatccaa aaaagaaaaa gatgcaagtt cagcaaaagt cattgacagt actttttctt
     9181 tgaaagaatt atatgcagaa aagcaagaca gtattgcaaa attatccaaa gaggaacagg
     9241 aacaaattaa aaaaatggag aattttaaaa tccgaatgat tattgatgaa gaaaataaaa
     9301 tcatgaaata tatattattt gctgatttta aatcgatgga tgaattgcaa aacatgacat
     9361 ctcccatcaa tacttttaaa tcaattggtg caggaggaag tgaagagtta actaaaaata
     9421 atcagttaac taatgatgcg ttgacaacct tttcttatga tgggaaaata tttaaaaaga
     9481 cagttcaaaa atctaatttt aaagaagatt ttaaagaagc tttaaaaaaa gaaaacagta
     9541 ctgacgaaga tatagatgta gaagaaattg cggaaggggt agatgaatct atgaatatga
     9601 tttttgagca atccagttat acaataatat atcaatttcc aaaacctatc aaaagtgttt
     9661 ctgtagaaaa tgctgtaatt agtgatgaca gaaaaacggt aactattcct tatcaaatga
     9721 aagattacat gagtaatcct gaaagtttaa attttgaaat aaattttgaa tagtatgaac
     9781 aaaaaaattc aacttcaaga tttaggatca aaagattata aactaacttg ggattaccag
     9841 gaagaattgt ttaaaaaaat tgttgacatt aaaattttga acagaagaga aggctcggaa
     9901 attgaaacac caaactattt tctttttgta gaacatcctc atgtatatac gttaggtaaa
     9961 agcggtgata tttcaaattt actgctttct gaaaaacaac tagaagcaaa aaaagcaact
    10021 ttctataaaa ttaatcgggg tggagatatt acgtaccatg gtcccggaca aattgttggt
    10081 tatccaatta ttgatttaga aaattatttt accgatattc ataaatattt acggttttta
    10141 gaagaagtca tcattctaac attagccgaa tataatataa taggaaaaag aagtgaaggt
    10201 gaaacaggag tttggttgga tgtaggcact ccttttgctc gaaaaatatg tgcgatgggt
    10261 gttcgggctt ctcgctgggt taccatgcat ggttttgctt taaatgtgaa tgccgattta
    10321 ggctactttg ataatatcat tccttgtggt attcgtggaa aagcagtaac ttctttacag
    10381 gtcgaactcg gtgttgaaaa agtagaggaa gaccaagtga aagaaaaaat agtaaaacac
    10441 ttttctaatt tatttgaggc tgaatttata aatccaattt caattgatca ggtaataatc
    10501 taaaagtcat tcggtggtat ttggtcgtgc cgtatttagc aattgcttct cgatgttcct
    10561 tggtaggata acctttgttt tgtttccaat tatacatagg aaattcttca tgaattagat
    10621 tcatgaattc atctcgataa gttttagcta aaacggaggc tgcagcaata cttacatatt
    10681 tactgtcccc tttaattatg cttgtagagg gaatagattt taaaagttct gtttctttag
    10741 gagtaaaaat cttaccattt ttttgaatca ttcctaattt tccatttaat ggtctgtttc
    10801 catccacaat aatatagttt ggaattgtct ttaatttcaa aatactttcc tgcatagctt
    10861 tcatggaagc attcaaaata tttatttcat caatttctac attatatata tgtgtaacgg
    10921 caaaagaaaa agcagtttcc tcaataaagg gtcttaattc aaatcggact ttctcggaca
    10981 actgcttaga atcgtttagt aattcaaaat atttttcaat caaattagcc tcgttttctt
    11041 gtccaaatcg taaagggcct atcactgcag ccgcggtcac cggtccggca agacatcctc
    11101 ggccggcttc atctgtgccg cattcaaatt catattccga gtatctaaat tttaacataa
    11161 aaatagttta ataaaatgag aattgtcacg caaccatttc tgttttaaag catcttctaa
    11221 tcaagtttgt gccataacaa cgaactaact gcaaaatttt taacaaaaat aaataatatt
    11281 attccgaagt aatatttgtt tatttaagaa attattagga agtttgcgga ataactaact
    11341 caaataaatt taatatgaga tcgaagttca aatggatttt tacgctttta ctagcgtttt
    11401 caatgcagtt ttcgttcgca caagagaaaa ctgtatcagg tactgttact gatgctagcg
    11461 gtatgccgtt gccaggtgca acagttgttg ttaaaggtac aagtagaggt acatcaacag
    11521 attttgatgg aaagtattcc attaaagcaa gtgtaggtga aacgttagat ttttctttcg
    11581 ttggctacaa agcagtttct gttaaagtag cagcatctaa cgtagtaaat gctactttaa
    11641 aagatgacac tgctttagat gaagttgtag ttacaggtgc tttcggtgtt aagaaaaaag
    11701 agaaagccgt aacctatgca gcacaatcta tcaaaggtga agcaatgact gaagccagag
    11761 aggctaactt agtgaatgct ttatccggta aagttgccgg ggttcaggtt acaagttctt
    11821 caggttctgt tggagcttct gccagaattg tattaagagg ggcttcttct atcactggaa
    11881 acaaccaacc gttgtttgtt attgatggtg ttccatttga taacacaaac tatggtaatg
    11941 ctgattctag tggtggtaga gatttgccaa acggtgctgc tagtattaat cctgatgata
    12001 ttgaaagtat caatgtttta aaaggaccta ctgctgctgc attatatggt ttaagagcta
    12061 gtaatggagt tattttaatt actacaaaat caggaaaaaa caaaggtaaa tttgaagttt
    12121 cgtttaattc aaacattact ttttcagatc ctttgaaatt gccaaactat caaaactcgt
    12181 atggtcaagg agcaactagt gatttctttc aattcgttga tggatcaggt ggtggttaca
    12241 atgatggagt agatgaaagt tggggtgctc cactagatgt gggtttaaac tttgttcaat
    12301 gggattctta taaagtaggt ggagctccat taccatgggt ttctcatcct gataacgtga
    12361 aagattttta tgataccggt gtgtcaatta c
//
LOCUS       DAOTCW010000063        11870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3421_length_11870_cov_38.518070, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000063 DAOTCW010000000
VERSION     DAOTCW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11870
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3421_length_11870_cov_38.518070"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="P5335_06670"
     CDS             complement(<1..317)
                     /locus_tag="P5335_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74595.1"
                     /translation="MRKFYFVKMLLFFYSFFIHAQDIAEKVINGTIICDSSSVEGVHV
                     LNLVNEKSTVTDRNGHFSIEAKQGDLLVFSAVHLNYWRKSVKENDFKTGFLEVIMTPK
                     EVK"
     gene            complement(426..1292)
                     /locus_tag="P5335_06675"
     CDS             complement(426..1292)
                     /locus_tag="P5335_06675"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRZ74596.1"
                     /translation="MKNQGLIFFLFLLFLTQGFSQVVIKGSVANFSTKEPLAYVNIGI
                     RNKEVGTISDKNGNFVLTINQDISLDELVIFSHIGFETIELSLKNLSKQNEIWLVPSV
                     NELNEVVLISPKTWTKKIGRTSKGAGMMHISFYTTYEKEINVELGREKGMLFDIKNDC
                     RIDNLNFCISGNEFKSIKFRMNIYNLKHGIPYENILNKNIIIEVKNGQTDWVQFDLKP
                     YEIYLEKELEQVAITLTWLESEKRDDKNWRFAFYAAMLPYFTMYNRDKSMATWSKSDA
                     CISMYVNTTCYK"
     gene            1435..2142
                     /gene="pepE"
                     /locus_tag="P5335_06680"
     CDS             1435..2142
                     /gene="pepE"
                     /locus_tag="P5335_06680"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HRZ74597.1"
                     /translation="MKNLIIASTSTIHEGSYLDYLLPNLKVHFAACQSILFIPYARPS
                     GISYEAYTQKVASVFNSIGISVKGIHEFSNPIEAIEQAEGIFTGGGNTFLLVSQLYEN
                     KVMSVLKQTIEKGTPYLGTSAGSNICGLTMKTTNDMPIIYPSSFNTLGVIPFNLNPHY
                     LDPDVNSTHMGETRETRIKEFHQFNTPAVLGLREGSWLEVKGNSILLRGSLTARLFRQ
                     NQEPVELESGSELNYLK"
     gene            complement(2189..2261)
                     /locus_tag="P5335_06685"
     tRNA            complement(2189..2261)
                     /locus_tag="P5335_06685"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2226..2228),aa:Gly,seq:tcc)
     gene            2406..2846
                     /locus_tag="P5335_06690"
     CDS             2406..2846
                     /locus_tag="P5335_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRZ74598.1"
                     /translation="MLTIREITALETYSVRHPVLRKGKPVESCHFDDDELHSTIHLGL
                     FINCALIGVISLFKNKNVNFTDKKQFQIRGMAVLKHHQKMGYGIKLVKEAEHIIQKYN
                     CNLLWFNARETAVDFYKKMGYTVIGESFIINDIGTHFVMYKKLI"
     gene            2862..4439
                     /locus_tag="P5335_06695"
     CDS             2862..4439
                     /locus_tag="P5335_06695"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRZ74599.1"
                     /translation="MKKNFLLFLALVLISCNDDKNKKADKDTTPIVTLTKEGKILAID
                     STVLHKSKDSTVIDFYHSNNNKTFWLAFNCRESLIDELKNVEDEGLNRADFDLKKIIK
                     FENTIGTLSDKALIEYDILLTENLSKYVKKVANGSLNPKELYKDWDLKDNKINPKKLL
                     LNFQRKDSFDYALYAVKPNHIVYRKLKHALKLINQFPDKNFEVLEAKDKIVYKDTHDL
                     LPEIKKRLVYWHDLKMPDSITSIYDENTLIGVKKFQSRHGLAPDGVIGKGTLSALNFS
                     KKKRKEQIIVNMERWRWFPKQFEKEYILINIPDYTLNVVKKNDTIRTHRIIVGKSSRS
                     TPILYSKLSYVVFNPTWTLPPTILKEDVIPAATKNRGYFATKNITIYNGNTEVSPDDW
                     NPAKAKSYRYVQPPGSSNSLGLVKIMFPNKHSVYLHDTNSRGYFGRENRSLSSGCIRV
                     QNPFELTEYLLEDSINWTKAKIDEVLAEAETKNVNFKKDIYLHILYWTAWSEGDKLQF
                     RDDIYRLDFDLYKKLRN"
     gene            complement(4428..5135)
                     /locus_tag="P5335_06700"
     CDS             complement(4428..5135)
                     /locus_tag="P5335_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HRZ74600.1"
                     /translation="MIYKLIPALFFGLLSFTTPTKNTTDPKIIAENTTATFESKVNLL
                     YNNLNSNNYKLPQLESFSKALEGFYQLKQKGLIQNNILTLIDFSLSSNVKRLWVIDMN
                     SNTILFQSLVAHGRNSGEEFATKFSNKAESYQSSLGFYITGEVYQGKHGQSLRLDGLE
                     KGINDKARDRAVVIHGADYVSDSFIKQNKRLGRSQGCPALPEELTPEIINTIKNKSCL
                     FIYHPSGSYKISSKLIS"
     gene            5402..>7302
                     /locus_tag="P5335_06705"
     CDS             5402..>7302
                     /locus_tag="P5335_06705"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRZ74601.1"
                     /translation="MKHFSQFFLFVFLFFGIDVAFGQKKSVQAIRITENIVVDGTLNE
                     SSWEKAPLAKDFVMYEPDNGALEKSEKRSEVKIIYNDNGIYIGATLYDNETTKIEKEI
                     TQRDIFGTADIFGIFLNGFNDGQQDFRFFVTAAGVQLDCLATENGEDFSWDAIWKSDI
                     SVTDFGWVVEMEIPYAALRFSKNDKQIWGLNIFREIRRDRHNYVWSHIDRAKGSVINQ
                     AGTLEGIENIKTPTRLFFIPYTSGYIESNDKTTNSTGKIGMDIKYGINDSFTLDAILV
                     PDFGQTAFDNVELNLGPFEQQFNENRPFFTEGTDLFSKGDLLYTRRIGGPPSTYPTTN
                     ENETVTDYPNTVDLINAVKISGRTQDGLGIGFLNAATAKTYAKIINNETNETREELVE
                     PHTNYNVLVFDQRFNQNSSVSFINTNVTRFGDFRDANVTGIVYNLNSKGNRFNVSGDV
                     KMSSIYDDEKPNGHTAFLNLAKTFGRYRYSVASKYVSKDFDNNDLGINFVTNYHNVYA
                     NFNYRIVKPTKTYNSFQLNSNYYLEIENTTGKLQENWINVNVNSSTLKNDYIGYGFLV
                     SPFVTYNFYEPRVPGRYLAFPATGNAWVFFSSNYNRRFAIDIEPSVRFFDQKDRINYS
                     LYASPRYRF"
     assembly_gap    7303..7402
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7403..7962
                     /locus_tag="P5335_06710"
     CDS             <7403..7962
                     /locus_tag="P5335_06710"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRZ74602.1"
                     /translation="QKDRINYSLYASPRYRFNDKFTLIYNASFSKQNSDIGWVDQVDS
                     EIILAERNRFTFNNGFSGKYALNNKMTINLTSRYYWSFAENIQFHTLQEDGTFIPNDT
                     YNADKDSNFNVWNFDVSYSWWFAPGSQITALYRNNAQYFTNEINKDFGSNANNLIDNS
                     LNHVFSVSLRYFIDYNKAKNWFKKA"
     gene            8016..9533
                     /gene="gpmI"
                     /locus_tag="P5335_06715"
     CDS             8016..9533
                     /gene="gpmI"
                     /locus_tag="P5335_06715"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRZ74603.1"
                     /translation="MNKKVILMILDGWGHSPDPKVSAIDNANTPFIDSLYKNYPNAEL
                     RTDGLNVGLPEGQMGNSEVGHMNLGAGRIIYQELTRINLAVKNKTLNDEKVLQDAFSY
                     AKINHKKVHFLGLVSDGGVHSHTSHLKGLLDAAQDYGLNNVYVHAFTDGRDVDPKSGK
                     GYIEDLEKYCSKSKAKLASVCGRYYAMDRDKRWERVKIAYDLLVNGVGTLSTNASETI
                     QKSYDADITDEFIQPILITDSNGNALATIENGDVIIFFNFRTDRGRQLTEALSQKDFH
                     EQNMHKLNLYYVTMTNYDETYHNVKVIYNKDNITETLGEILEKNHKKQIRIAETEKYP
                     HVTFFFSGGREEPFEGETRILRNSPKVATYDLKPEMSAFELKDALVPELQKESVDFVC
                     LNFANGDMVGHTGVMEAAIKACEAVDTCVKEVIETALTHNYTTIVIADHGNCETMINP
                     DGSPNTAHTTNPVPVILVDKDIKSIKSGVLGDIAPTILHLMGIVKPEAMTRESLV"
     gene            9663..10334
                     /locus_tag="P5335_06720"
     CDS             9663..10334
                     /locus_tag="P5335_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRZ74604.1"
                     /translation="MKNILEGLKVNISNKIYIKDPETSDLGKNILENSILLINEIGFD
                     NFTFKKLGEKIGSNESSIYRYFENKHKLLLYLSSWYWGWLEFKIVLATTSISNPMEKL
                     IKALEIVTEETTEDNNHPHINEVVLNNIVVCEFSKSYLTKEVDDENKEGYFLIYKRVI
                     NRIVEMVVEVNPNYPTPRSLISTVIEGSLHQHFLKNHFKTITDCNDKTTPTEFFTALV
                     IKQIS"
     gene            10339..>11870
                     /locus_tag="P5335_06725"
     CDS             10339..>11870
                     /locus_tag="P5335_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRZ74605.1"
                     /translation="MKKESTSLTPLKRFINLLKLDKKDIYQVFFYAIFSGIVSLSLPL
                     GIQAIINLIQGGRVSLSWIILVLVVIIGVAFNGVLKLMQLRITETIQQKIFIRSSFEF
                     SYRFPKIKYTEFNNYYPPELANRFFDTLTVQKSVAKLILDYSESLLQIIFGLLLLSVY
                     HPLFILFGLLLFIVLYLIFKLSFQTGLKTSLTESKYKYKVAHWIQEIARNYFGFKSKV
                     EADFALSKNDKLVTEYLKSRESHFQIIKRQFIQLIGFKILISSGLLLIGGFLVINQQI
                     NIGQFVAAEIIILLIINSVEKIIIGLETFYDLLTSIEKIGQIVDMELEEFSDEEHNYC
                     YKNILFEIDSLNFSFPDSNKKVLNNISLKINQGETIYLDGNNGSGKTTFMRILAGLIQ
                     TNTNSIYLNDENYKKINITQYRSQIGIITAGQTTFEGSILENITFYNNSVAPERLKWV
                     LDKVKLTDEIKKMPNGLDEFILTDGKQLSSSITQKIVLARAIINHPNILFLEDPFDKM
                     DN"
BASE COUNT         4191 a         1845 c         1846 g         3888 t
ORIGIN      
        1 aatttcactt ctttgggagt cattatcact tctaaaaatc ctgttttaaa atcattttct
       61 ttcacacttt ttcgccaata gtttaaatga accgccgaaa aaactaacaa atcaccttgt
      121 ttagcttcaa ttgaaaaatg gccatttcga tcagtaactg ttgatttttc gtttactaaa
      181 tttaaaacat gaacaccttc aaccgaactt gaatcacaaa taattgttcc gttgattact
      241 ttttcagcta tatcttgagc atgaataaaa aaggaataaa agaaaagcaa cattttgaca
      301 aagtaaaatt ttctcataaa ctatctaaga aattcaattg catttagaac tgataattat
      361 ttatactctt catcaaattt attttaaaaa aatagcactt catcaagcaa ccactgaaga
      421 ttaacttatt tataacaagt tgtatttacg tacatactta tacaggcatc cgattttgac
      481 caagttgcca tagatttatc tctattgtac atcgtaaaat aaggcaacat tgccgcataa
      541 aacgcaaaac gccaattttt atcatccctt ttttcgcttt ccaaccaagt taaagtaatc
      601 gctacttgtt ctagttcctt ttctaaataa atttcataag gttttaaatc aaattgaacc
      661 cagtccgttt gaccattctt gacctcaatt ataatatttt tattaaggat gttttcataa
      721 ggaattccgt gttttaaatt gtaaatattc attcggaatt taatcgattt aaattcattg
      781 ccgctgatac aaaaattaag attgtctatt ctacaatcat ttttaatgtc aaaaagcatc
      841 cctttctccc ttcctaattc tacattaatt tctttttcat aagtagtata aaaactaata
      901 tgcatcattc cggcaccttt tgatgtgcga cctatttttt tcgtccaggt tttgggagaa
      961 attaaaacca cttcattcaa ttcgtttacc gatggaacta gccaaatttc gttttgcttg
     1021 gatagatttt tcaaacttaa ttcaatcgtt tcaaatccaa tgtgcgaaaa aatcactagc
     1081 tcatccaaac tgatatcttg attgatggtt agtacaaaat ttccgttttt atcagaaatg
     1141 gttccaactt ctttatttct tattccaata tttacatagg ctaatggctc ttttgttgaa
     1201 aaattggcaa cactaccctt gataactact tgtgaaaaac cttgagttaa aaaaagtaaa
     1261 aacaaaaaaa agattagtcc ctgatttttc ataattgaat ttcttaattg aaaaactaat
     1321 ggtaaaaata ttgtgttttc ttcccaatat cctcccaaag actttgtaat tatttgttaa
     1381 actgaaacag ttttaaaaga gataaaccct tacttttgta taaattccaa tgaaatgaaa
     1441 aacctaatta ttgccagcac ctcaactata cacgaaggaa gttaccttga ttatttattg
     1501 ccaaatctga aagtacattt tgctgcttgc caatcgattc tattcattcc ttatgcccga
     1561 ccgagtggaa tttcttatga agcatatact caaaaagttg cttctgtttt taactccatt
     1621 ggtatatccg ttaaaggaat tcacgaattt tcaaatccaa ttgaagccat tgagcaagcg
     1681 gaaggcattt ttacgggagg cggaaacacc tttttattag tttcacaatt atatgaaaat
     1741 aaagtgatgt ctgttttgaa acaaaccatc gaaaagggca cgccttattt aggtacaagt
     1801 gccggtagta atatttgtgg gttaaccatg aaaaccacga atgatatgcc catcatttat
     1861 ccttctagtt ttaatacgtt gggtgtcatt ccttttaatt taaatccgca ttatttagac
     1921 cctgatgtga attccacaca tatgggcgaa acgcgtgaaa ccagaatcaa agaatttcat
     1981 caattcaata ctcctgccgt tttaggatta cgagaaggaa gctggttaga agtaaaagga
     2041 aactccattt tattgagagg atctttaacc gctcgtttat tcagacaaaa ccaagaaccg
     2101 gttgagttag agagtggaag tgagctgaat tatttaaagt agattataaa aaaagaaaac
     2161 cccaaactta cgtttgaggt tttagcagag cggaagacga ggctcgaact cgcgacaacc
     2221 agcttggaag gctggagctc taccaactga gctacttccg catttgtgag tgcaaatata
     2281 gataattact ttactttgat gcaaattttt tttgaaaaaa aatatcttaa agctaactta
     2341 aattgtaatg gttttattct taaattgtta tatctttaga ttaatgtaat taatgctact
     2401 gaatcatgct caccatcaga gaaataacgg cattagaaac ctattctgtt cgacatcctg
     2461 ttttgagaaa aggtaaaccg gttgaaagtt gccactttga tgacgacgaa cttcattcta
     2521 caattcattt agggctgttc attaattgtg ctctaattgg tgtaatttca ttatttaaga
     2581 acaaaaatgt caactttaca gataaaaaac aattccaaat tcgcggaatg gctgttttaa
     2641 aacaccatca aaaaatgggc tacggcataa aactcgttaa agaggctgaa catattattc
     2701 aaaaatacaa ttgtaatttg ctttggttta atgcccgtga aacagcagtt gatttttata
     2761 aaaaaatggg atatacagtt attggtgaat cttttattat aaatgacatt ggcactcatt
     2821 ttgtaatgta taaaaaattg atttagccca ttatacattt tatgaaaaaa aatttcctct
     2881 tatttcttgc attagtatta atttcttgta atgacgacaa gaataaaaag gcagataaag
     2941 acacaacccc tattgttact cttactaaag aaggtaaaat tttagcaatt gattcaactg
     3001 tacttcataa aagtaaagat tcgaccgtaa ttgattttta tcattcaaat aacaacaaaa
     3061 cgttttggtt agccttcaat tgcagagaat cacttattga tgagcttaaa aatgtagaag
     3121 acgaaggatt gaatcgtgcc gattttgatt tgaaaaaaat aatcaaattt gaaaatacta
     3181 ttggcacctt gtcggacaaa gcattaatag aatatgatat attactaacc gaaaatctta
     3241 gtaagtatgt caaaaaagtt gctaatggaa gtctaaatcc caaagaactc tacaaagatt
     3301 gggatttaaa agacaacaaa attaatccga aaaaattact tttaaatttt caacgaaaag
     3361 attcatttga ttatgcttta tatgctgtaa aaccaaatca tattgtttac agaaaactta
     3421 aacacgcgtt aaaactcatt aaccaatttc ctgataagaa ttttgaagta ttagaagcaa
     3481 aagataagat agtttataaa gatacgcatg atttgctacc tgaaattaaa aaacgattag
     3541 tctattggca tgatttaaaa atgccggatt cgataacttc catttacgat gaaaacacct
     3601 taataggcgt gaaaaaattt caatcccgcc atggattagc tcctgatggt gtgataggaa
     3661 aaggaactct ttctgcctta aacttttcga agaaaaaaag aaaagagcaa atcattgtca
     3721 acatggaacg ttggcgatgg tttcctaaac aatttgaaaa agaatatatt cttatcaata
     3781 ttcctgatta cacactcaac gtagttaaaa aaaatgacac tatccgaacg catcggatta
     3841 ttgttggtaa atctagcaga agcacaccca ttttgtattc aaaattgagc tatgttgtct
     3901 ttaatcctac atggacactt cctcccacca tattaaaaga agatgttatt ccggctgcta
     3961 caaaaaacag aggctatttt gcaactaaaa acatcactat ttataatggc aatacagaag
     4021 ttagtccgga cgattggaat ccggcaaaag caaaatcata tcgctatgtt caacctcccg
     4081 gttcttcaaa ttctttagga ttggtaaaaa tcatgttccc aaataagcac tccgtttatt
     4141 tacacgacac caattcaaga ggttattttg ggagagaaaa tcgctcgtta agttccggtt
     4201 gtattcgagt acaaaatcca tttgaactta cagaatatct tttagaggat tcaattaatt
     4261 ggacaaaagc aaaaattgat gaagtgcttg cagaggctga aaccaaaaat gtaaatttca
     4321 aaaaagacat ttatttacac atcctttatt ggacggcatg gagtgaaggt gataaacttc
     4381 aatttagaga tgatatttat cgcctggatt ttgatttgta taaaaaacta cgaaattaat
     4441 tttgaagaaa tcttataact tccggatgga tgatatatga ataaacagga tttgttttta
     4501 atcgtattaa ttatttcagg agtcaattct tcaggcaaag caggacaacc ttgacttctt
     4561 cctaatcgtt tattctgttt gataaacgaa tcagaaacat aatctgcccc gtgaattacg
     4621 acagctctgt ctcttgcttt atcgttaatc cctttttcaa ggccgtctaa acgcaacgac
     4681 tgaccatgtt tgccttgata aacctctccg gtaatataaa aacctaagct gctttgataa
     4741 gattctgctt tattagaaaa ttttgtagca aattcttctc ctgagtttcg tccgtgagca
     4801 actaatgatt gaaataaaat cgtgttggaa ttcatatcaa taacccacaa tctttttacg
     4861 tttgaagaca aactaaaatc aatcaatgtt aaaatattat tttgaatcag ccctttttgt
     4921 tttaattgat aaaatccttc caaagcctta gaaaaacttt ctaattgagg aagtttataa
     4981 ttatttgaat tcaaattatt gtataataaa ttcactttcg attcgaaagt tgcagtagta
     5041 ttttcagcaa taattttggg atctgtggta ttttttgtag gcgttgtaaa ggataataat
     5101 ccgaaaaaca aagccggaat aagcttgtat atcattggta ttctttaggt taaaactcga
     5161 tttgggttgg ccaaaaatat aaataaaatc aatacaacca aattaatttt gtcttaatta
     5221 ttcatttttt ggaaataatt cattttagtg aatcaattga ataacgcttt ctttttcaaa
     5281 ttagtatcta aaaactgtaa caattcaata aaaaaacgtc tttatagtat attatcttaa
     5341 ggaaatgata aagtgattgc acttggaaaa gctttatcta attttgaaca ttatttactt
     5401 tatgaagcat tttagccaat ttttcttatt tgtttttctc ttttttggaa ttgatgttgc
     5461 ttttggccaa aaaaaatcag ttcaggctat ccgaataaca gaaaatattg ttgtagacgg
     5521 cacattaaac gaatcatcgt gggaaaaagc tccacttgca aaagattttg taatgtatga
     5581 accggataat ggtgctttag aaaaatccga aaagagaagt gaagttaaaa ttatatataa
     5641 cgataacggt atttatattg gagcaacgct ttatgataat gaaactacta aaattgaaaa
     5701 ggaaatcacc caaagagata tttttggaac tgccgatata tttggaatat ttctaaatgg
     5761 ttttaacgac ggccaacagg attttcgttt ttttgttaca gctgcaggag ttcaattgga
     5821 ctgtttggca actgaaaatg gggaagattt ttcttgggat gccatttgga aaagtgacat
     5881 ttccgttact gatttcggtt gggttgttga aatggaaata ccttatgccg ctttacgctt
     5941 ttctaaaaat gataaacaaa tttggggact taatattttt agagaaattc ggcgagacag
     6001 acataattat gtttggagtc atatcgatag agcgaaaggc tctgttatca accaagcagg
     6061 aactttagaa ggtattgaaa atattaaaac tcctacccga ttatttttta ttccttatac
     6121 ttccggttat attgaatcaa acgataaaac cacaaatagt acaggtaaaa ttggaatgga
     6181 tattaaatac ggaatcaatg attctttcac tttagatgca attttagttc ctgattttgg
     6241 acaaactgca tttgacaatg tcgagttaaa tttaggccca tttgaacaac aatttaatga
     6301 aaacagaccc ttttttacag aaggaactga tttgttttca aaaggtgatt tgctctacac
     6361 aagaagaatt ggcgggcctc cctccaccta ccctacaaca aatgaaaatg aaaccgtgac
     6421 tgattatcca aatacagtcg acctgattaa tgcggtaaaa atttcaggaa gaacgcaaga
     6481 tgggcttggt attggatttt taaatgctgc aacagcaaaa acgtatgcta aaataattaa
     6541 caacgaaaca aatgaaaccc gcgaagaact ggttgaacct cacactaatt ataatgtttt
     6601 agtgtttgat caacgtttta atcagaattc gtcagtttct tttatcaata caaatgtgac
     6661 aaggtttggt gattttcgtg atgcaaacgt taccggaatt gtttataatt taaattcaaa
     6721 gggaaataga tttaatgttt caggagatgt aaaaatgagc agtatttatg atgatgaaaa
     6781 accaaatggt catacggctt ttttaaattt agctaaaaca tttggccgat atcgttattc
     6841 tgttgcttca aaatatgttt caaaagattt tgacaataat gatttaggaa tcaattttgt
     6901 tacgaattat cataatgttt atgctaattt taattaccga atagtaaaac ctactaaaac
     6961 ctacaattcc tttcaactta attcaaatta ctatttagaa attgaaaata caaccggaaa
     7021 attgcaggaa aattggatta atgtcaatgt aaactcctcc actttaaaaa atgactatat
     7081 cggttatggc tttttagtaa gtccttttgt tacctataat ttttatgaac ctcgtgttcc
     7141 gggcaggtat ttagcttttc cagccactgg aaatgcctgg gtatttttct cttcaaatta
     7201 caatagacga tttgctatcg acattgagcc ctccgttcgt ttttttgatc aaaaagatag
     7261 aattaattat agtttatatg ccagtccaag ataccgattt aannnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnatcaaaaa gatagaatta attatagttt atatgccagt
     7441 ccaagatacc gatttaatga caaattcact ttaatttata atgcatcttt ttcaaaacaa
     7501 aacagtgata ttggttgggt cgatcaagtt gattctgaaa ttatattagc cgaaagaaat
     7561 cgatttactt tcaataacgg attttcaggg aaatatgcac tgaataacaa aatgactatc
     7621 aatttaactt ctcgttatta ttggtcattt gctgaaaaca tacagtttca caccttacaa
     7681 gaggacggta catttatacc aaatgatact tataatgccg ataaagattc taattttaat
     7741 gtttggaatt ttgacgtttc ctattcttgg tggtttgctc cgggaagtca gattactgct
     7801 ttatatcgaa acaatgcaca atacttcaca aatgaaataa acaaagattt tggctcaaac
     7861 gcaaacaact taatagataa cagtttgaac catgtttttt ctgtaagtct tcgctatttt
     7921 attgattata acaaagcaaa aaattggttt aaaaaagcct aattcttcgc ttataaagct
     7981 atacattctt tatctttgtt ctaaatttta atcttatgaa caagaaagta atcctgatga
     8041 ttttagacgg atggggacat tcgccagatc caaaagtttc cgcaattgat aatgccaata
     8101 ctccttttat tgattctctt tataaaaact atcctaatgc cgaattacga acagatggtt
     8161 taaacgtagg gcttcctgag ggtcaaatgg gaaattctga agtaggtcat atgaatttag
     8221 gagccggtag aattatttat caagaactta ctcgaataaa tttagccgta aaaaataaaa
     8281 ctttaaatga tgaaaaagta ctacaagatg ctttttctta tgccaaaatc aatcataaaa
     8341 aagtgcattt tttaggtttg gtttctgatg gtggagttca ctctcatact tctcacctaa
     8401 aaggattatt agatgcagct caagattatg gtttaaataa tgtttatgtt catgccttta
     8461 cagatggacg cgatgtggat ccaaaatcag gaaaaggata cattgaagat ttagaaaaat
     8521 attgttcaaa atcaaaagca aagttagctt ctgtttgtgg cagatattat gcgatggatc
     8581 gagataaacg ctgggaacgc gtaaaaattg cttatgatct attggttaat ggcgttggaa
     8641 ctctttcaac taatgcatca gaaaccattc aaaaaagtta tgatgctgat attactgatg
     8701 aatttatcca accaattttg ataaccgatt ccaatggaaa tgctttggca accattgaaa
     8761 atggtgatgt aataatcttt ttcaatttca gaacagacag aggtcgacaa cttaccgaag
     8821 ctttatctca aaaagatttc cacgaacaaa atatgcataa attgaatctg tactatgtta
     8881 caatgaccaa ttatgacgaa acctatcata acgtaaaagt gatatataat aaagataata
     8941 tcaccgaaac cttaggagaa attctggaaa aaaatcacaa aaaacaaata agaattgccg
     9001 aaacagagaa atatccgcat gtaactttct ttttttccgg cggtcgagaa gaaccgtttg
     9061 aaggcgaaac acgaatttta agaaactcac caaaagtggc tacttacgac ttaaaacccg
     9121 aaatgagtgc ctttgaatta aaagatgctt tagtccctga actgcaaaaa gaatcagtcg
     9181 attttgtttg cttaaatttt gccaatggcg acatggttgg ccatacaggt gtgatggaag
     9241 ccgcaataaa agcttgtgaa gctgtagaca cttgcgtgaa agaagtgatt gaaacggctt
     9301 tgacacataa ttatacaact atagtaatag ccgatcacgg aaattgtgaa acgatgatta
     9361 acccagacgg cagtccaaat acagctcata ccacaaaccc ggtcccggtt attttagtag
     9421 acaaagatat aaaatcaatt aagagtgggg ttttgggcga catcgctcct acaatacttc
     9481 atttaatggg aattgtaaaa ccggaagcga tgacaaggga atcattagtt taaaactatc
     9541 tgatatttta attaaagcca ttccaatcga atggcttttt tattaaaaat aagttaatta
     9601 ctttcaaaat caatagtaat actattattt ttgcggtagt aaatattacg aaataaaatt
     9661 taatgaaaaa tattttagag ggtctaaaag taaacatcag taataaaatc tatataaaag
     9721 atcctgagac ctcggatttg ggcaaaaata ttttagaaaa cagcattcta ctaattaatg
     9781 aaattggttt tgacaatttc actttcaaaa aactaggaga aaaaataggt tccaacgaaa
     9841 gttcgatata tcgctatttt gaaaacaaac ataaactact tttatattta tcttcttggt
     9901 attggggctg gcttgaattt aaaatagtgc tagccactac tagcatttct aatccaatgg
     9961 aaaaattaat taaagcttta gaaatagtta cagaagaaac tactgaagac aacaatcatc
    10021 cgcatataaa tgaggtggtt ttaaataaca ttgttgtttg tgaattttca aagtcctatt
    10081 taactaaaga ggtggatgac gaaaacaaag aaggctattt tttaatatac aaaagagtaa
    10141 tcaatcgtat tgtagaaatg gttgttgaag taaatcctaa ttatccgact cccagaagtt
    10201 taatttcaac agttattgaa ggttcattac atcaacattt tttgaaaaac cattttaaaa
    10261 caattaccga ctgcaatgac aaaaccacac caacagagtt ttttacagca ttggttataa
    10321 aacaaatcag ttaatcaaat gaaaaaagaa tcaacttcgt taaccccttt aaaaagattt
    10381 attaatctat tgaaattaga caaaaaagat atttatcaag tattttttta tgctattttc
    10441 tcaggaattg ttagcctttc tttaccattg ggaattcaag ctatcatcaa tttaattcaa
    10501 ggcggaagag taagtttgtc atggattata ttagtattag ttgttattat tggagtagcc
    10561 tttaatggtg ttttaaaact catgcaacta agaattactg aaactattca acaaaagata
    10621 tttattcgtt cctcttttga attctcttat cgttttccaa aaattaaata tactgaattt
    10681 aacaattatt atcctcctga attggcaaat cgtttttttg acacacttac cgttcaaaaa
    10741 agtgtggcca aattaatttt agattattca gaatcattat tacaaatcat tttcggatta
    10801 ttattacttt ctgtttacca tcctctattc atactttttg gattgctatt attcattgtt
    10861 ttatatttaa tttttaaact gtcctttcaa accggattga agacgagttt aactgaatca
    10921 aaatataagt acaaagttgc tcattggata caagaaatcg ctcgaaatta ttttggattt
    10981 aaaagcaagg ttgaagctga ttttgcatta tcaaaaaatg acaaattggt gactgaatat
    11041 ttaaaatcaa gagaaagcca ctttcaaatc atcaaaagac aatttattca attgattggt
    11101 tttaaaattt taatatcttc cggtttattg ttaattggtg gattcttagt gattaatcaa
    11161 caaatcaata ttggacaatt tgtagctgct gaaattatca ttttgttaat tatcaattcc
    11221 gttgaaaaaa taatcattgg tttagaaact ttttatgatt tgctgacttc gattgaaaaa
    11281 atcgggcaaa ttgtagacat ggaattagaa gaattttcag atgaagagca caactattgt
    11341 tataaaaata tattatttga aattgatagt ttgaactttt catttccaga ttctaataag
    11401 aaagtattaa acaatatttc tttaaaaatc aatcaagggg aaaccatata tctggatgga
    11461 aataacggtt caggcaaaac aactttcatg cgaattttag ccggtttaat tcaaaccaat
    11521 accaattcga tttatttaaa cgatgagaac tataaaaaaa ttaatattac acagtatcgt
    11581 tcacaaatag gtatcatcac ggccggacaa acaacttttg aaggaagtat tcttgaaaac
    11641 atcacatttt acaataactc ggtagctcct gaacgcttaa aatgggtttt ggataaagta
    11701 aaactgacag atgaaattaa aaaaatgccc aatggcttag acgaatttat attaaccgac
    11761 ggaaaacaat tatcttcttc tattactcag aaaatagtat tggctagagc gataataaat
    11821 catccaaaca tattgttttt agaagatcct tttgacaaaa tggacaatga
//
LOCUS       DAOTCW010000064        11792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3459_length_11792_cov_36.037318, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000064 DAOTCW010000000
VERSION     DAOTCW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11792
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3459_length_11792_cov_36.037318"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="P5335_06730"
     CDS             complement(<1..215)
                     /locus_tag="P5335_06730"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HRZ74606.1"
                     /translation="MAYKIQKTEEEWKALLGMERYKILRQKGTEFPHTGKYNLHFEKG
                     TYCCAACNEPLFESNSKFDAHCGWPSF"
     gene            complement(215..688)
                     /gene="msrB"
                     /locus_tag="P5335_06735"
     CDS             complement(215..688)
                     /gene="msrB"
                     /locus_tag="P5335_06735"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRZ74607.1"
                     /translation="MKKLILMLSIFTLTACQGQKKDQKNSKLKVVKTEAEWKSELTPE
                     QYEVLRKKGTESAFTGKYWDHFEKGQYVCTACENPLFDSQTKFESDCGWPSFNQAIKG
                     SVIYENDYSFGMSRVEVMCAKCGGHLGHVFDDGPKESTGKRFCTNSISVKFIPKK"
     gene            complement(706..1569)
                     /locus_tag="P5335_06740"
     CDS             complement(706..1569)
                     /locus_tag="P5335_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRZ74608.1"
                     /translation="MSKKVKSDNQSLQIPKLILLTAKILQSISLNLVTRFAAKLFTTP
                     IKHKIPKRELHMDNNSIQQLVKIPAINKEIMMYHYGKSENKILLVHGWSGRGTQLVKI
                     ADNLLELGYSTISFDAPAHGKSNGHFTIMTEFIASILELEKQYGPFDYAIGHSLGGMS
                     ILNALKQNLKVKKAVIIGSGDIIQDIVVDFVKKLQLKPIIADKLQKHFETKFGEPMNN
                     YSAFVAAKNIEIPILIIHDADDVEVSVKAAHHIHEHLKNSKIIITKELGHRKILGNQK
                     VIDEINEFIKY"
     assembly_gap    1738..1837
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1838..3730
                     /locus_tag="P5335_06745"
     CDS             <1838..3730
                     /locus_tag="P5335_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HRZ74609.1"
                     /translation="PQLDGGVFYIKRIVNQNVSVSANVFSDGHDVIACCVKFKHELEK
                     KWSEVRMSPTENDEWSVDFKVIKQGVYYYFIEGWVDYALNWQHGTERKIKDNQHVNSE
                     LLEGAEYCLAILKEVSKEEKVYLNEVIKSFQDEKLYGKAVEFALSNELNQIFLKYPTR
                     TLANTSAELKVYVDRKKALFSTWYEFFPRSASENPGKHGTFKDCERLLPRVLEMGFDT
                     LYFPPIHPIGEVNRKGKNNATNAENGDVGSPWGIGSKYGGHKSTHPELGSIDDFKSLV
                     NKAQSMGIEIAMDYALQAAPDHPYVKYFPQWFKWRPDGTVQYAENPPKKYQDIQPIYF
                     ESGDWKNLWKELLDVALYWIEECGIKIYRVDNPHTKPFYFWGWLISEIKKKHPDVLFL
                     AEAFTRPKIMNELAKQGFTQSYTYFTWRNSKAELIEYLTELTQTEQREFFRPNFWPNT
                     PDILPYALQNGHESVYLQKYFLAATLSSSIGIYGPAYEFMVQQAMPGKEEYLNSEKYE
                     VYHWDWEAKNKLTTIITRINNIRKQHESLQQTNNIVFCKVENDQIMAYYKFNDSKTDH
                     TLMICNLDPYYPKQAMVRLPLEEIGNQPVRITDLITGNSYLWDKEWNFVELHPALPFH
                     LFKIDR"
     gene            3733..5355
                     /locus_tag="P5335_06750"
     CDS             3733..5355
                     /locus_tag="P5335_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose synthase"
                     /protein_id="HRZ74610.1"
                     /translation="MDTTNQDTFASTFEFKTDWKTCFEDEDFVKVFSSDILENYIINK
                     RWYGGKASTLKYIDVVDFFKIASKKNTYYGVLLEVNFKEAFFQHYFMPLAFMSKEELD
                     TSTVIAPVKMGNQEGFLVDALHQEDFRKMLFDNIIKSKETKGQKLIFHKGSTVENQEY
                     DSSRFMGLEQSNTSIIYNDTYVLKIFRRIYVSTNPDYEISRFLTERMHFPNSPAYTGS
                     INLALSEGNITLGLMQELVPNQGDAWKFMLEQTDGVFDNLKRKKIKIDKLPDIQMFKR
                     LKINEIPPEIIDWVGLSLFLRIQTLALRTAEMHIALGSDIHETAFTPTTYNGDYTVWL
                     KNRMLYQFQNRLNIIENSLHKLDGLALELAHQFMENKKIVRKHFVDFDWTKMKSERIR
                     IHGDYHLGQVLVNGDDFYILDFEGEPESTIRDRKVKQPPLKDVAGMFRSFHYAIYATI
                     FNNKEKYPFEQEELFKAGEILYKYFVGVFLETYIEKAQSGNLNIGYNHEIDFLLKYCL
                     LEKAVYELGYELNSRPRWAVIPLRGIQSIMNY"
     gene            5413..>6292
                     /locus_tag="P5335_06755"
     CDS             5413..>6292
                     /locus_tag="P5335_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRZ74611.1"
                     /translation="MNLVQPHSLFTDFDIDLFKAGKHFRLYEKLGAHLTEVNGVKGVY
                     FAVWAPSARSVSVVGDFNYWIQGEHQLYVRWDASGIWEGFIPGLDKGTTYKYKIQSNN
                     NGIVTEKADPFALYCEKPPHTASVIWDLDYKWKDKKWMDSRKDKNGLDKPYSVYEVHL
                     GSWKRNVEENRFLSYIELAEDLVNYVKETGFTHVEFMPIMEYPYDPSWGYQLVGYFAP
                     TSRFGKPQDFMLLVDKLHQAGIGVILDWVPSHFPDDAHGLGFFDGSNLYEHPDRRKGY
                     HPDWKSLVFNYGRNEVR"
     assembly_gap    6293..6392
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6393..7475
                     /gene="glgB"
                     /locus_tag="P5335_06760"
     CDS             <6393..7475
                     /gene="glgB"
                     /locus_tag="P5335_06760"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HRZ74612.1"
                     /translation="YHPDWKSLVFNYGRNEVRSFLISNAIFWLQHYHVDGLRVDAVAS
                     MLYLDYSRKDGEWEPNIYGGRENLDTISFLKDFNEAVYANFEGVQTIAEESTSFPMVS
                     RPTFLGGLGFGMKWMMGWMHDTLQYFQKESVYRKYHQNDLTFSMTYAFSENFMLPLSH
                     DEVVYGKKSILGRMTGDEWQRFANLRLLYGYMFTHPGGKLLFMGAEFGQSAEWKFDGS
                     LDWNLLQFAYHDGIKKCITDLNGLYKNNPALFEKQFTAEGFEWINYSDAQNAVLSYIR
                     KGNNEKENLVVVCNFTPVVRENYRIGVPTKGKLTELFNTDKKEYGGSGIGNPKAIKIE
                     KEPWNGREFSVALTLPPLSVTVFKIS"
     gene            7621..10020
                     /locus_tag="P5335_06765"
     CDS             7621..10020
                     /locus_tag="P5335_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRZ74613.1"
                     /translation="MITNTELEYKGDQFPTHIIAYNQDVDSIYFYTENNVVLKITILR
                     DSMIRFRYTTKGYFNKDFSYAIDKGQSHGYNDLHVAEEKLYYTITTSKIICRINKENA
                     KVSLFDLEGFSILEDEIGFHWEESYEYGGNIVKMSKKANDGECYYGMGDKATHLNLKG
                     KRLENWATDQYAFLKEQDPLYKVVPFYIGLHHNKAYGVFFDNTFRTFFDFCHERRNVT
                     SFWAEGGEMNYYFIYGPKMKDVVIAYTHLTGKPELPPLWTLGYHQCKWSYYPESKVKE
                     ITSTFRKLQIPCDAIYLDIDYMEGFRCFTWSEEYFPNPKRMVSELAKDGFKTVVIIDP
                     GIKIDKEYSVYQEALEKDYFCKRADGPYMKGKVWPGECNFPDFTNPEVREWWAGLYKE
                     LISDIGVKGVWNDMNEPAVMDVPGKTFPMDVRHDYDGNPCSHRKAHNVYGTQMARATY
                     EGVKRFAYPKRPFIITRSAYSGSQRYTSSWTGDNVASWEHLWVANIQMQRMSISGMGF
                     TGSDIGGFAEQPSGELYARWIQLGVFHPFCRTHSSGDHGEQEPWSFGEEVIDITRKFV
                     NLRYQLLPYLYTMFWKYVNEGEPMLKPLVYFDQEDIQTLYRTDEFIFGNQILVCPILE
                     PNAIGRRMYLPKGNWYNYWTNELLEGKKELWVATDYDQIPIFIKEGAIIPKYPVQQFV
                     GEKEFEEITLDVYYKFGKEKSVLYEDAQDGYDYNKGRFSLRTFNLTGKDKELIIQLHE
                     EGAFMTNYSKFRINLIGLPFKVSSIEIDNEKVSFGSVSYDGKNTLLVDKFFTELHIVG
                     K"
     assembly_gap    10228..10327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10328..10451
                     /locus_tag="P5335_06770"
     CDS             <10328..10451
                     /locus_tag="P5335_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HRZ74614.1"
                     /translation="GGAPPEFMSTHPSHDTRVSNLSKWIPEAKAEAAKYGVIFK"
     gene            10514..>11792
                     /locus_tag="P5335_06775"
     CDS             10514..>11792
                     /locus_tag="P5335_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRZ74615.1"
                     /translation="MSTLAKGSKKLLNAWAFYDWANSVYPLVISSSIFPLYYGFLFPK
                     ENPNVLFLGFEFKSTALISFVTAFAFLCVAFFSPLLSGIADFAGNKKRFMQFFCYMGA
                     LSCIGMYWFTLDNIYFGITCFFFGLLGFWGSLVFYNSYLPDIAFKEQQDSLSAKGYSF
                     GYVGSVILLLINLYMILGKEGEEAMHAMRLAFAMTGIWWILFAQYAFYYLPKGNKNAE
                     KITKDVVLNGFRELRKVQTHLCQNLKLYRYLVAFFVYSMAVQTVMLVATYFGEQEINW
                     VGDSEKTSGLIISILVIQLVAVLGATLTSKASEKFGNIPTLIFVNTIWAIICVFAYFV
                     TEPIHFYITAGFVGLVMGGIQALSRSTYSKFLPKTDDTASYFSFYDVAEKIGIVIGMV
                     IYGLIDQITGSMRNAIVFLGVFFVAGLILLLRVP"
BASE COUNT         3858 a         1644 c         2100 g         3890 t
ORIGIN      
        1 tcaaaagatg gccaaccaca atgtgcgtca aactttgaat tactttcaaa taaaggctca
       61 ttacaagctg cacaacaata agttcctttt tcgaaatgaa ggttatattt tccagtatgc
      121 ggaaattcag ttcccttttg gcgaaggatt ttatatcttt ccatacctaa cagggctttc
      181 cattcctcct cagttttttg aattttatat gccattattt ttttggaata aatttaactg
      241 aaattgaatt tgtacaaaag cgttttcccg tactttcttt aggtccgtca tcaaaaacat
      301 gtcctaaatg cccaccacat tttgcacaca tgacttcaac tcttgacatt ccaaaactat
      361 aatcattttc ataaattaca gaacctttaa ttgcttgatt aaacgaaggc caaccgcaat
      421 cagattcaaa ttttgtttgt gaatcaaata atggattttc acatgccgta cagacatatt
      481 gacctttttc gaaatgatcc cagtattttc ccgtaaatgc actttctgta ccttttttac
      541 gcaatacttc atattgttct ggagttaatt cagatttcca ttcggcctca gttttaacaa
      601 ctttcagttt tgaatttttt tgatcctttt tttgtccttg acaagctgtt aaagtgaata
      661 ttgacagcat taaaattaat tttttcatag cagattttta attgtttaat acttaataaa
      721 ttcatttatt tcatcaataa ctttttgatt tccaagtatt ttacgatgac ctaactcttt
      781 cgttattatg attttactgt tttttagatg ctcgtgtatg tgatgtgctg ctttaacaga
      841 aacttcaaca tcatcagcat catgaataat tagtattgga atttcaatat ttttagcagc
      901 tacaaaagct gaatagttat tcattggttc accaaatttg gtttcaaaat gcttttgtaa
      961 tttatcagct ataattggtt ttaattgtaa tttttttaca aaatcaacaa caatatcctg
     1021 aataatatca ccactaccga taatcactgc tttttttact tttaagtttt gtttgagggc
     1081 atttaaaatc gacatacctc ctaatgaatg ccctattgca tagtcaaaag gaccatattg
     1141 tttttctaat tctaaaatag aagcgataaa ttcggtcata atcgtaaaat gaccattact
     1201 ttttccatgg gctggagcat caaaacttat agtagaatag cctaattcaa gtaaattatc
     1261 agctattttc acaagttgtg ttcctcgacc cgaccaacca tgaaccaata gaattttatt
     1321 ttcactctta ccataatgat acatcattat ttctttatta attgccggaa tttttaccaa
     1381 ttgttgaata ctattattat ccatatgcaa ttctcttttt ggaattttat gtttaatagg
     1441 agttgtaaac aattttgccg caaatcgtgt cactaaattt aaagaaattg attgtaatat
     1501 tttagctgta agtagaatta gcttaggaat ctgcaaggat tgattgtcag atttgacttt
     1561 ttttgacatt tcaataaatt ttattgcaag gtagaattta aaattatttt ttataaattt
     1621 atagttcata aatattactt aaaatgcaaa accaaacccg aattattatt gaaaatgtaa
     1681 aaccgcaatt agacggtggg gtcttttata taaaaagaat agttaatcaa aatgtttnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg caattagacg gtggggtctt
     1861 ttatataaaa agaatagtta atcaaaatgt ttcagtatca gcaaatgttt tttcagatgg
     1921 acacgacgtg attgcttgtt gtgtgaaatt taaacatgaa ttagaaaaaa agtggtctga
     1981 agtaagaatg tctccaactg aaaatgatga atggagtgtt gattttaaag ttattaaaca
     2041 aggggtttat tattatttta ttgaagggtg ggttgattat gctttgaatt ggcaacatgg
     2101 tactgaacga aaaattaaag ataatcaaca cgttaattca gaattattag aaggggctga
     2161 atattgttta gctattttga aggaagtttc caaagaagaa aaagtatatt taaatgaagt
     2221 tataaaatca tttcaggatg aaaaattata tggcaaagcc gttgaatttg cgttgagtaa
     2281 tgaattaaat caaatttttc taaaataccc aacccgaaca ctggcgaata cttccgctga
     2341 attaaaagtt tacgtagacc gaaaaaaagc attgttcagc acttggtatg agttttttcc
     2401 tcgttctgct tctgaaaacc caggtaagca tggaacattt aaagattgtg aacgattgtt
     2461 gcccagagtt ctagaaatgg ggttcgacac attgtatttt ccacctattc atccaattgg
     2521 agaagtaaat cgtaaaggta aaaataatgc aactaatgcc gaaaatggag atgtaggttc
     2581 accctgggga attggttcga aatatggtgg gcataagtca acacatcctg agttgggttc
     2641 aattgatgat tttaaatcat tggttaataa agctcaatca atgggaattg aaattgcaat
     2701 ggattatgct ttacaagctg caccagacca tccatatgtt aagtattttc cacaatggtt
     2761 caaatggcgt ccggatggaa cagttcaata tgctgaaaat ccacccaaaa aataccaaga
     2821 tattcaacca atttattttg aaagtggtga ttggaaaaat ctttggaaag aactattgga
     2881 tgtagcttta tattggattg aagaatgcgg tattaaaatt tacagagttg ataatccaca
     2941 cacaaaacct ttttattttt ggggttggtt gataagtgaa ataaaaaaga agcatcctga
     3001 tgttttgttt ttagctgaag cctttactag acctaaaatt atgaacgaat tggcaaaaca
     3061 aggttttaca caatcgtata cttatttcac ctggcgaaat tctaaagcag aattaattga
     3121 atatttaacg gaattaaccc aaacggaaca aagagaattt ttcagaccaa atttttggcc
     3181 taatacaccc gatatcttgc cctatgcatt gcaaaatggt catgaatcgg tatatcttca
     3241 aaagtatttt ttagcagcta ctttaagttc aagtatagga atttatggac cagcatacga
     3301 atttatggtt caacaagcta tgccgggtaa ggaagagtat ttaaattctg aaaaatatga
     3361 agtttatcat tgggattggg aagccaaaaa taaacttacc acaattataa caagaataaa
     3421 taatattcgt aaacagcatg aatcattaca acaaaccaat aatattgttt tttgtaaggt
     3481 tgaaaatgat caaataatgg catattataa attcaatgat tctaaaacag atcatacatt
     3541 aatgatatgt aatttagatc catattatcc aaaacaagca atggttcgtt tacctttgga
     3601 ggaaataggt aatcaaccag ttcgtattac tgatttaatt acaggaaaca gctatttgtg
     3661 ggataaagaa tggaattttg ttgaattgca tccggcttta ccttttcatt tatttaaaat
     3721 tgaccgataa ttatggatac aacaaatcaa gatacttttg ccagtacatt cgaatttaaa
     3781 actgattgga aaacttgttt tgaagatgaa gattttgtca aagttttttc ttcagatata
     3841 ttagaaaatt acatcatcaa taaaagatgg tatggaggaa aagcaagtac tttaaaatat
     3901 atcgacgttg tagatttttt taaaatagct tccaagaaaa acacctatta cggtgtgcta
     3961 ttagaagtta attttaaaga agcttttttt caacattatt tcatgccact ggcttttatg
     4021 tcgaaagaag agttggatac tagtactgtt attgctcccg taaaaatggg aaatcaagaa
     4081 ggttttttag ttgatgcttt acatcaggaa gattttcgta aaatgttgtt tgacaatata
     4141 ataaaatcta aagaaactaa aggacaaaaa ctcatttttc acaaaggatc aactgttgaa
     4201 aaccaagaat atgactcttc acgttttatg ggattggaac aaagtaatac atcaattata
     4261 tataatgaca cctatgtttt aaagattttc agacgtatat acgtgagtac aaatcctgat
     4321 tatgagatta gtcgtttttt aactgagcgg atgcattttc caaattctcc tgcttatacc
     4381 ggaagtatca atttagcttt gtctgaagga aatattactc taggtttaat gcaggaattg
     4441 gttcccaatc aaggagatgc ttggaaattt atgttggagc aaaccgatgg tgtttttgat
     4501 aatttgaaac gtaaaaagat aaaaatcgac aagttaccgg atatacaaat gttcaaaaga
     4561 ttaaagataa atgaaattcc gccggaaata attgattggg ttgggttgag tttgttttta
     4621 cgcattcaaa cgttagctct tcgaacagca gaaatgcata ttgctttggg tagtgatata
     4681 catgaaaccg cctttactcc aactacttat aatggcgatt ataccgtttg gttaaaaaac
     4741 agaatgctat atcagtttca aaacagatta aatattattg aaaatagttt gcataaatta
     4801 gatggattag ctttagagct ggctcatcaa tttatggaaa acaaaaaaat agtccgaaaa
     4861 cattttgtag attttgattg gacaaagatg aaatcagaac gaattagaat tcacggtgat
     4921 tatcatttag gacaagtttt agtaaacggc gacgattttt atattcttga ttttgaaggc
     4981 gaaccggaaa gtaccattcg tgaccgaaaa gtaaaacaac ctccgttgaa agatgttgcc
     5041 ggtatgttcc gttcatttca ttatgctatt tatgcaacaa tttttaacaa taaagaaaaa
     5101 tatccttttg aacaagaaga actatttaaa gccggagaaa ttttatataa atattttgtt
     5161 ggagtctttt tagaaactta catcgaaaaa gctcaatccg gcaatttgaa cattggttat
     5221 aatcatgaaa ttgattttct tttaaaatat tgcttgcttg aaaaagcggt ttatgaatta
     5281 gggtatgaac tcaattccag accacgctgg gcagttattc ccttaagagg aattcagagt
     5341 attatgaatt attaaaaatt gctttagaat aaaataaaat tatagaacct aaatagggtt
     5401 aataaaaaac atatgaactt agtacaaccg cattctcttt ttaccgattt cgatattgat
     5461 ttatttaagg ccggaaaaca ttttagatta tacgaaaaat taggagctca tctcacggaa
     5521 gttaatggtg ttaaaggggt ttattttgcc gtttgggcac catccgcacg atcagtttca
     5581 gtagttgggg attttaatta ttggattcaa ggcgaacatc aattgtatgt tcgttgggat
     5641 gcttccggca tttgggaagg atttattcca ggattagaca aaggaactac ttataaatat
     5701 aaaattcaat ctaacaacaa cggaatagtt actgaaaagg cagatccatt tgctttatat
     5761 tgtgaaaaac cgcctcatac cgcttctgtt atttgggatt tagactataa atggaaagac
     5821 aaaaaatgga tggattccag aaaagacaaa aatggcttag ataaaccgta ttctgtttac
     5881 gaagttcatt tgggttcttg gaaacgcaat gtcgaagaaa atcggttttt aagctatatc
     5941 gaattagcag aggatttagt caattatgta aaagaaaccg gttttactca tgtcgagttt
     6001 atgccaatta tggaatatcc ctacgatcca tcatggggtt atcaattagt gggttatttt
     6061 gcaccgactt cccgctttgg aaagccacag gattttatgc ttttggtcga taaattgcac
     6121 caagccggaa ttggtgttat tttagattgg gtgccttccc attttcctga tgacgctcac
     6181 ggacttggat tttttgatgg ttcaaacctt tatgaacatc ccgacagacg taaaggttat
     6241 caccccgatt ggaagagttt agtgtttaat tacggtcgta atgaggtacg ttnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntatcaccc cgattggaag agtttagtgt
     6421 ttaattacgg tcgtaatgag gtacgttcct ttttgatcag taatgccatt ttctggttgc
     6481 aacattacca tgtagatgga cttcgtgtag atgcagttgc ttccatgtta tatttagatt
     6541 attccagaaa agatggtgaa tgggaaccaa atatttatgg aggaagagag aatttagata
     6601 ccatcagttt cttaaaagat tttaatgaag ctgtatatgc taattttgaa ggagttcaaa
     6661 caattgctga agaaagtact tcttttccaa tggtttcccg tcctacattt ttgggaggat
     6721 taggtttcgg aatgaagtgg atgatgggct ggatgcatga tacgcttcaa tactttcaaa
     6781 aagaatctgt ttatcgaaaa taccatcaaa atgatttaac tttttcaatg acgtatgcct
     6841 tttcggagaa ctttatgttg cctctttcgc atgatgaagt tgtttatggg aagaaatcaa
     6901 ttttaggtag gatgactggt gacgaatggc aacggtttgc taacttacgt ttgctttatg
     6961 gttatatgtt tactcatccg ggtggaaaat tattattcat gggagcagag tttggccaaa
     7021 gtgcagaatg gaaatttgat ggaagtttag attggaattt gttacaattt gcttaccacg
     7081 atggtattaa aaagtgtatt acagatttaa acggactgta taaaaataac ccggcattat
     7141 ttgaaaagca atttacagct gaaggatttg aatggattaa ttattctgat gcacaaaatg
     7201 cagttttgag ttatattcgt aaaggaaata atgaaaaaga aaacttggtt gtcgtttgta
     7261 attttactcc cgttgttaga gaaaattatc gaataggagt tccaacaaaa ggaaaattaa
     7321 cagaactctt taatactgac aaaaaagaat atggcggaag tggaattgga aaccccaaag
     7381 ccattaaaat agaaaaagaa ccttggaacg gaagagaatt ttcagttgcc ttaactttac
     7441 caccattgag tgttacagtt tttaaaattt cttaaaaata aataatagaa atccctctaa
     7501 ttaagagggt tttttgtttt attaatatac gcaaacgttt gagttaataa atcctaaaat
     7561 caaaatagtt gttcaaacta tcatctttgt aaatttggca acattacatg accataactt
     7621 atgataacta acacagaatt agaatataaa ggggatcaat ttccaaccca tataattgct
     7681 tataatcaag atgttgatag catttatttt tatactgaaa acaacgttgt tttaaaaatc
     7741 accattcttc gtgacagtat gattcgattt cgttatacca caaaaggata ttttaataaa
     7801 gacttttctt acgcgattga caaaggtcaa tcacacggat ataacgacct tcatgttgct
     7861 gaagaaaaat tgtattacac catcactacg agcaaaataa tttgtagaat taataaagaa
     7921 aatgcaaaag tatccctatt tgatttagaa ggtttttcta ttttagagga tgaaattgga
     7981 tttcactggg aagaaagtta tgaatatggc ggaaatatcg tcaaaatgag taagaaagca
     8041 aatgatggtg aatgttatta tgggatggga gacaaagcta ctcatttgaa tttaaaagga
     8101 aaacgtttag aaaattgggc aacagaccaa tatgcctttc taaaagagca agacccttta
     8161 tacaaagtgg ttccttttta catcggattg catcataata aagcatatgg agtatttttc
     8221 gataatacat tcagaacatt ttttgatttt tgtcatgaac gaagaaacgt aaccagtttt
     8281 tgggctgaag gaggcgagat gaattattat tttatctatg gtccaaaaat gaaagatgtg
     8341 gttattgcat atactcattt aaccggaaaa ccggaacttc cgcctttatg gaccttgggt
     8401 taccaccaat gtaagtggag ttattatccg gaaagtaaag taaaagaaat cacgtcaacg
     8461 tttagaaaat tacaaatacc atgtgatgcc atttatttag atatagatta catggaaggc
     8521 tttcgatgtt ttacatggag tgaagaatat ttcccgaatc cgaaaagaat ggtatcggaa
     8581 ttagcaaaag atggatttaa aacggttgtt attattgatc ccggaattaa aattgacaaa
     8641 gaatattcag tttatcagga agcattagaa aaagattatt tctgtaaacg tgccgatgga
     8701 ccttatatga aaggaaaagt ctggccgggt gaatgtaatt ttccggattt tacaaatcct
     8761 gaggtacgtg aatggtgggc aggtttatat aaagaattga tttctgatat tggtgtcaaa
     8821 ggcgtttgga atgacatgaa cgaacctgct gtaatggatg ttcctggaaa aactttccca
     8881 atggatgttc gacatgatta tgatggaaat ccatgtagtc accgaaaagc tcataatgtt
     8941 tatggaacac aaatggctcg tgcgacctat gaaggcgtaa agcgatttgc ttatccgaaa
     9001 agacctttta ttattacacg ttctgcttat tcaggttcac aacgatatac ttcttcatgg
     9061 acaggagata atgttgcttc atgggaacat ttatgggtag caaatatcca aatgcaacgc
     9121 atgtcaataa gcggaatggg ctttaccggt tctgatattg gtggttttgc agaacaacct
     9181 tccggtgaat tatatgctcg atggatacaa ttaggagtgt ttcacccttt ttgtagaaca
     9241 cattcatccg gagatcacgg tgaacaggaa ccttggtctt ttggggagga agtcattgat
     9301 attactcgaa aattcgtaaa tcttcgttac caattacttc cctatttata caccatgttc
     9361 tggaaatatg tcaatgaagg ggaacctatg ctgaagccat tagtttattt tgaccaggaa
     9421 gatattcaaa cgctttacag aacggatgag tttatttttg gaaatcaaat tttggtttgc
     9481 ccaattttag agcctaatgc aattggtcgt agaatgtatt taccgaaagg gaattggtat
     9541 aactattgga cgaatgaatt gttagaaggg aaaaaagaac tttgggtagc taccgattat
     9601 gatcaaattc caatttttat aaaagaagga gctatcattc ctaaatatcc tgttcagcaa
     9661 tttgttggcg aaaaagagtt tgaagaaatt accttggatg tttattataa attcggaaaa
     9721 gaaaaatccg ttttatatga agatgcacaa gatggttatg attataataa agggagattt
     9781 tctttacgaa cattcaattt gaccggaaaa gataaagagt tgattattca attgcatgaa
     9841 gaaggagcgt tcatgacaaa ttattcaaag tttagaatta atttaatagg attacctttt
     9901 aaagtttcat ccatagaaat tgataatgaa aaagtctctt ttggtagtgt ttcttatgat
     9961 ggaaaaaaca cattgttggt ggataaattc tttacagaac tacacatagt gggtaagtag
    10021 tttaattgtc gtacttttat agaaaacaaa attttataca attatgaaaa gaattatggc
    10081 gtttggttta ttcggttttt ttgtacttat tatggcttgt gctacaaacc cattaacagg
    10141 taaaagtacg ttagcattag tagataatag ttcaatattt ccatctgctt ttcaacagta
    10201 tgatgctttt ttgaaagaaa acaaagtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnagg aggagctcca ccggaattta tgagcacaca tccatcacat gatacaagag
    10381 tttctaattt aagtaaatgg attcctgagg cgaaagcaga agctgctaaa tacggagtga
    10441 tttttaaata aaaatttcta tattcgaagc tgttctgaaa agaacagttt ttttatgact
    10501 aaaatctaaa tttatgagca cacttgcaaa gggaagtaaa aaattattga atgcatgggc
    10561 cttttatgat tgggcaaatt ctgtttatcc attagtaatt tcttcttcaa tttttccatt
    10621 gtattatggt tttttatttc caaaagaaaa tccaaatgta ctttttttag gtttcgaatt
    10681 taaaagtaca gcactcatta gttttgttac agctttcgct tttttatgtg ttgctttctt
    10741 ttccccttta ctttccggta ttgctgattt tgcaggaaat aaaaaacgtt tcatgcagtt
    10801 tttttgctat atgggagcat tgtcatgtat tggaatgtat tggtttacat tggataacat
    10861 ttattttgga attacatgtt tcttttttgg tttattaggt ttttggggaa gtttagtatt
    10921 ttataattcg tatttaccgg atattgcttt taaagaacaa caggacagtt taagtgcaaa
    10981 aggatactct tttggctatg taggaagcgt tattttactt ttaattaatt tgtatatgat
    11041 tctaggtaaa gaaggtgagg aagccatgca tgccatgcga cttgcttttg caatgacagg
    11101 aatttggtgg atactatttg ctcaatatgc cttttattat ttacccaaag gaaacaaaaa
    11161 tgctgagaaa ataaccaaag atgtagtttt aaatggattc cgagagctta gaaaagtaca
    11221 aactcatcta tgtcaaaacc ttaaattgta taggtattta gtagcttttt tcgtttatag
    11281 tatggcagtt caaacagtta tgcttgtggc aacttatttt ggtgaacagg aaattaattg
    11341 ggttggagat agcgaaaaaa cttccggttt aatcattagt attttagtta ttcaattagt
    11401 tgccgttttg ggagctactt taacctcgaa agcatcagaa aaatttggaa atattccaac
    11461 attgattttt gtcaatacaa tttgggcaat catttgtgtg tttgcttatt ttgtaactga
    11521 accaattcat ttttatataa cagccggctt tgtaggatta gtaatgggag gaattcaagc
    11581 tttgtcccgt tcaacttatt caaagttttt gcccaaaacc gatgatacag cttcctattt
    11641 tagtttttat gatgtagccg agaaaatagg aattgtaata ggaatggtaa tttatggctt
    11701 gatagaccaa attacgggaa gtatgcgaaa tgcaatagtg tttttaggtg ttttctttgt
    11761 agcaggtttg attttattac tcagagtacc aa
//
LOCUS       DAOTCW010000065        11692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3510_length_11692_cov_44.150382, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000065 DAOTCW010000000
VERSION     DAOTCW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11692
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3510_length_11692_cov_44.150382"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="P5335_06780"
     CDS             complement(<1..599)
                     /locus_tag="P5335_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRZ74616.1"
                     /translation="MSLPKTIPEVSFFRFLNHANNILKNPLPFHAANFKKLGDIFRLN
                     IGLGKSVLFCRDAGLLQHALQKNQKNYTKSYIQTRDLAKYIGKGLLTAEGEHWQRQRK
                     LIQPAFHKSQLVLLIETIQETILEELKKIKTGKPMDVFPVFNDLAFQTVVKSIFNIAI
                     SDEDIASLQHTTEATQKMLVQELRQPFLVWWFNLSGKTK"
     gene            complement(623..1645)
                     /gene="ruvB"
                     /locus_tag="P5335_06785"
     CDS             complement(623..1645)
                     /gene="ruvB"
                     /locus_tag="P5335_06785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRZ74617.1"
                     /translation="MNEYLDPSNKSFNADELDLEKKLRPLSFDDFTGQEQVLENLKIF
                     VEAANQREEALDHTLFHGPPGLGKTTLANILANELGVGIKITSGPVLDKPGDLAGLLT
                     NLENRDVLFIDEIHRLSPIVEEYLYSAMEDFKIDIMIESGPNARTVQINLNPFTLVGA
                     TTRSGLLTAPMRARFGIQSRLQYYNTELLTTIVQRSSMILKVPISMEAAIEIAGRSRG
                     TPRIANALLRRVRDFAQIKGNGSIDIEIARYALKALNVDAHGLDEMDNKILTTIIDKF
                     KGGPVGLSTLATAVSESGETIEEVYEPFLIQEGFIMRTPRGREVTEKAYKHLGKIRTN
                     IQGGLF"
     gene            complement(1757..3559)
                     /locus_tag="P5335_06790"
     CDS             complement(1757..3559)
                     /locus_tag="P5335_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRZ74618.1"
                     /translation="MSAHDHANEQDHGHEHEHHHKDTFITKYIFSIDHKMIAKQYLIT
                     GLIMGIIGVAMSLLFRMQLAWPEESFKIFNVLLGDTFAPDGVMRNDIYLALVTIHGTI
                     MVFFVLTAGLSGTFSNLLIPLQIGARDMASGFMNMISYWLFFLSSVIMVCSLFVESGP
                     ASAGWTIYPPLSALPQAIPGSGTGMTLWLVSMAIFIASSLMGSLNYVVTVINLRTKGM
                     SMTRLPLTIWAFFVTAIIGIVSFPVLLSAALLLIFDRSFGTSFFLSDIYIAGEVLHYQ
                     GGSPVLFEHLFWFLGHPEVYIVLLPALGITSEIIATNSRKPIFGYRAMIASIIAIAFL
                     STIVWGHHMFVSGMNPFLGSVFTFTTLLIAIPSAVKAFNYITTLWKGNLQMNPAMLFS
                     IGLVSTFITGGLTGIILGDSTLDINVHDTYFVVAHFHLVMGISALYGMFAGIYHWFPR
                     MFGRMLNKNLGYVHFWVTAICAYGVFFPMHFIGMAGLPRRYYTNTAFPLFDDLQNVNV
                     IITVFALVGGAFQLVFIWNFFHSMFYGEKSVQNPWRSNTLEWTAPVEHMHGNWPGEIP
                     EVHRWPYDYSKPGHAEDFVSQIVPMGPDEKELHH"
     gene            complement(3589..4746)
                     /locus_tag="P5335_06795"
     CDS             complement(3589..4746)
                     /locus_tag="P5335_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRZ74619.1"
                     /translation="MTSLLVIIVLVFVSVAIWQLTKIFDLTQIGDNAENYQVANDNDN
                     SVNGYLMFAFLVFIYLTTIYCVVKFGHFPLMSNPASEHGVEVDRLMWISMILIFFVQT
                     VTQALLHYFAFKYRGKKGNTALYFADNNKLEAIWSIIPAITLAGLILYGLYAWTNIMF
                     VDEETENPMVVEIYAKQFGWEVRYAGKDNVLGKANVRYIEGINTLGVDLADPNAQDDI
                     QASELHLPKGTKVVFKIRSQDVLHSAYMPHFRAQMNCVPGMVTQFAFTPTVTTVDMRN
                     SKAMIEKVDAINKIRTEKSKELVAKGLEALDPYTFDYLLLCNKICGKSHYNMQMKIVV
                     ESPEDFKKWLGGQTTLVQNIKAQEQPVEADKATIDTATVVKDSTVIVQVVK"
     gene            complement(4767..6101)
                     /locus_tag="P5335_06800"
     CDS             complement(4767..6101)
                     /locus_tag="P5335_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HRZ74620.1"
                     /translation="MYTLSSKLKVFSIVLMLVGLLGIGYSFISAPKTTAEVEKILEAD
                     AHGEHGNAHGATAHEAHGKDAKADAEHQEHLKHVLHQLENKPWAAIYVAALFFMLISL
                     GALAFYGIQNVAQAGWSPILFRVMQGITAYLPVGSVIFFVLLLLSAFHYNHLFAWMDH
                     DLLDPANPKYDKLIANKSGYLNEYFWLIRAAIFLIGWNLYRYFSRKNCLAQDEASDNT
                     FYKKNFKMSAAFLVFFIVTESIMSWDWIMSVDPHWFSTLFGWYVFASFFVSGITVIAL
                     VTLYLKSKGYLEHVNTSHIHDLAKFMFGISIFWTYLWFSQFMLIWYSNIPEEVTYFVT
                     RIEQYNLPFFGMLALNFIFPFLILINTDFKRITWIIVMGGVVILFGHYIDFFTMIMPA
                     TVGDQWFIGVGEISSLLFFFGLFLLVVFTALTKAPLVPKRNPFIEESKHFHY"
     gene            complement(6127..6690)
                     /locus_tag="P5335_06805"
     CDS             complement(6127..6690)
                     /locus_tag="P5335_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRZ74621.1"
                     /translation="MKSLFKITLLVGLALSVTSCFDSKSPNYQFFPNMYQSVGYETYA
                     ESEAFNGKNQLQGQTAQEPPTGTIKRGFEIYEYENTTAGYELAKANLKSPIDSISEKE
                     ATKGKDLYVIYCSICHGEQGNGKGKLVEREKFLGVPSYADRPITEGSVFHVITYGLNA
                     MGSHANQLSQHERWLVTDYVLQLKSKL"
     gene            complement(6700..7224)
                     /locus_tag="P5335_06810"
     CDS             complement(6700..7224)
                     /locus_tag="P5335_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HRZ74622.1"
                     /translation="MSTKVIQALYNDDDILMDAVKETRKAHHHIEEVYTPFPVHGLDK
                     AMGLAPTRIAIASFMYGVVGLSFSIWMMNYIMIQDWPQDIGGKPSFSYIENMPAFVPI
                     MFELTVFFAAHLMVITFYMRSKLWPFKQAENPDVRTTDDHFVMEVSIHNNEDELASFL
                     KGTGAIELKVVEKH"
     gene            complement(7246..>7803)
                     /locus_tag="P5335_06815"
     CDS             complement(7246..>7803)
                     /locus_tag="P5335_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74623.1"
                     /translation="MRKVSNLEAYITIQHIELMNIVIMITGSIVGVAYITELFVAWYS
                     GVEYEQYAFLNRATGPYAWAYWAMMTCNVFSPQFMWFKKLRTSIMFSFVISIVVNIGM
                     WFERFVIIVTSLHRDYLPSSWTMFSPTFVDIGIYIGTIGFFFVLFLLYARSFPVIAQA
                     EVKTILKSSGEFYKKKREEEGHHNH"
     assembly_gap    7804..7903
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7904..8478)
                     /locus_tag="P5335_06820"
     CDS             complement(<7904..8478)
                     /locus_tag="P5335_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HRZ74624.1"
                     /translation="MSSHYEAPIRKPLVIGDKSYHDITVDVAAPVEGRANKQWWIVFS
                     IALTAFLWGVGCMIYTISTGIGTWGLNKTVGWAWDITNFVWWVGIGHAGTLISAVLLL
                     FRQKWRMAINRSAEAMTIFSVMQAGLFPIIHMGRPWLGYWVLPIPNQFGSLWVNFNSP
                     LLWDVFAISTYLSVSLVFWWTGLLPDFAMLR"
     gene            complement(8514..11579)
                     /locus_tag="P5335_06825"
     CDS             complement(8514..11579)
                     /locus_tag="P5335_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HRZ74625.1"
                     /translation="MSSNKKYWKSVEELNENSSIVETLRNNEFIEPIPTDEFLGDKDS
                     LSNSSTSRRDFLKYVGFSTAAASLAACEGPVIKSIPYVVQPEQIIPGVADYYATSMFD
                     GFDFANLLVKTREGRPIKIENNTIEGAKFTANARVHASVLSMYDNMRLKQPKVNQKDA
                     TWSEVDAKVKASLTDAKATGAQVVLLTGTLASPSTDKLVSEFLVNNPTAKHVIYDAVS
                     SSEALDAFQAVYGERALAEYEFEKASTIVSVGADFLGDWQGGGYDKGYAQGRVPKQGK
                     MSKHIQLEANMTLSGAAADKRIPMSVAEQKQALVKIFSIVTGTSVAVGKLANEAEIVK
                     IAEQLNQSGSKGVLVSGIDDKNAQLLVLAINAKLNSEAFNPNQTRQIRKGSNQAVAQL
                     VADLNGGKVHTLIMSGVNPVYSLAEGKAFADGLKKTKLSVAISLKEDETASLTNIAAP
                     APHYLESWGDVQIIKGTYSLTQPTIRPLFNTRQLQDNLLVWNGSDKTYYDFLKESWTG
                     VLVGKTWNQAVHDGIFVSATVSSNVASSADFSAAAATLAQAKPSSGLELNLYTKTGMG
                     DGQQANNPWLQEFPDPITRASWDNYLTISKADAEKYGVKNHNVANGGLNGSYVTVTVG
                     GTKLEKVPVIIQPGQAKGTVGLALGYGKKAALKEAMQVGVNAYTLYKGFNATQSATIA
                     LESGEHEFACVQLQKTLMGRGDIIKETTLTVFNAEKDAKVWNPPPMVSMDHKPIEATK
                     VDLWDSFDRSVGHHFNLSIDLNSCTGCGACVIACHAENNVPVVGKTEVRRSRDMHWLR
                     IDRYYSSEDTFAGDNKKKDEFDGLTGEKGSLSGFGELEHPADNPQVAFQPVMCQHCNH
                     APCETVCPVAATSHSRQGQNHMAYNRCVGTRYCANNCPYKVRRFNWFLYNKNNEFDFH
                     MNDDLGRMVLNPDVNVRSRGVMEKCSMCIQMTQATILKAKKEGRQVNADEFQTACSSA
                     CSTGSLAFGDVNNKESEVAKLVEDDRMYHLLEHVGTKPNVFYHVKVRNT"
BASE COUNT         3824 a         2232 c         1761 g         3775 t
ORIGIN      
        1 ttttttgttt ttcctgacaa attaaaccac caaacaagaa aaggttgcct tagttcttgc
       61 actaacattt tttgagtggc ttccgtagta tgttgtaaag aagcaatatc ttcatcagaa
      121 atagctatat taaaaataga ctttacaact gtttgaaaag ctaaatcatt aaaaaccgga
      181 aaaacatcca ttggctttcc tgtttttatt ttttttaatt cctctaaaat cgtttcttga
      241 atagtctcaa ttaaaagtac aagttggctt ttatgaaagg caggttgaat taatttacgt
      301 tgtctttgcc aatgttcccc ttctgctgtt agcaagcctt ttccaatata ttttgctaaa
      361 tctcttgttt gaatatagga tttagtataa tttttttgat tcttttgaag agcatgttgc
      421 aatagcccgg catctctaca aaacaatact gatttaccca atccaatatt taaacgaaag
      481 atatctccta atttcttaaa attggctgca tgaaaaggca atggattttt taaaatgtta
      541 ttggcatgat ttagaaacct aaaaaaagag acttcaggaa ttgttttggg taaactcata
      601 gtttttttgt tgcttagagc tattaaaata aaccgccttg gatattggtt ctgatttttc
      661 ccaagtgttt atatgctttt tcagtaactt ctctccctct aggtgttcgc atgataaaac
      721 cttcttgaat taagaatggc tcatatactt cctctatcgt ttcaccactt tctgaaacgg
      781 ctgttgccaa tgtggacaac cctaccggac ctcctttgaa tttatcgatt atagtcgtta
      841 atattttatt atccatttca tctaacccat gggcatccac attcaatgct tttaaggcat
      901 accgagcaat ttcgatatct atagatccat tcccttttat ttgggcaaaa tcacgtactc
      961 ttctcaataa tgcattagca attctaggag ttccgcgact tcgaccagct atttcaatag
     1021 ccgcttccat tgaaatggga acttttaaaa tcatcgaact acgttgaaca atggtggtca
     1081 ataattctgt gttataatat tgcaatcggc tttgaattcc aaatcgtgct cgcattggtg
     1141 ctgttaataa tccagaacgt gtagtagcac ctaccaaagt gaaaggattc aaattgattt
     1201 gtacggttct cgcatttgga ccggattcaa tcatgatgtc aattttaaaa tcctccatag
     1261 cagaatataa atattcttca acgattggac ttaaacgatg aatctcatca ataaaaagca
     1321 catctctatt ttctaaattg gtcaataaac cagctaaatc gcctggttta tctaaaactg
     1381 gacctgaagt tattttaatt ccaacgccta attcgtttgc taagatgttt gctaaagtag
     1441 ttttacccaa acccggagga ccatgaaata aggtatggtc aagggcttct tctcgttgat
     1501 tagctgcttc aacaaatatt tttaaatttt caagcacttg ttcctgacct gtaaaatcat
     1561 caaaagacag cggtcttaac tttttttcaa ggtcaagttc atccgcatta aaacttttat
     1621 ttgagggatc taaatattca ttcataaaaa caaagataga aaatgttttg tgttaagttg
     1681 tttttgaaac ttgataaaaa taaaaaagcc cttccaaaaa tgaaaaggct taaatatgaa
     1741 ttaataaact ataattttaa tgatgtaatt ctttttcatc aggtcccatt gggactattt
     1801 gagagacaaa atcttctgca tgtccaggtt tgctataatc ataaggccat ctgtgaactt
     1861 caggaatttc cccaggccag tttccatgca tatgctcaac gggagcagtc cattctaatg
     1921 tatttgatct ccaaggattt tgtactgatt tttcaccata gaacatactg tggaagaaat
     1981 tccaaataaa caccaattga aaagcaccac caactaaagc aaatacagta atgattacgt
     2041 ttacattttg taaatcatca aataacggga aagctgtatt ggtataataa cgtcttggta
     2101 aaccggccat tcctataaag tgcattggga agaaaactcc ataagcacag atagctgtta
     2161 cccaaaagtg cacatatcct aaatttttat ttaacattct accaaacatt cttgggaacc
     2221 aatgataaat tccggcaaac ataccataaa gagcggaaat acccataact aaatggaagt
     2281 gagctacaac aaaataagta tcatgaacat ttatatcaag tgtactatcc cctaagataa
     2341 ttccagttaa tccaccggtt ataaaggttg aaaccaaacc aattgaaaac aacatagctg
     2401 gattcatttg cagatttcct ttccacaaag tagtaatata attaaacgct tttaccgcag
     2461 atggaattgc aatcaataat gttgtaaagg taaataccga acctaagaat ggattcatac
     2521 cggaaacaaa catgtggtga ccccaaacaa tggtagacaa aaaggcaatt gcaataattg
     2581 aagctatcat tgcacggtaa ccaaagatag gtttacgaga attggtagca ataatttcag
     2641 aagtaattcc caaggcaggt aataatacaa tataaacctc agggtgacct aagaaccaga
     2701 ataaatgttc gaatagaaca ggagaaccac cttgataatg taaaacttca ccggctatat
     2761 aaatatcaga taagaaaaat gatgtcccaa aacttctgtc aaatattaat aataaagcag
     2821 cagataataa tactgggaaa gaaacaatcc caataattgc agtaacaaaa aatgcccaaa
     2881 tagttaaagg cagtcttgtc attgacattc cttttgttct taagttaatt accgtaacca
     2941 cataattcag tgaccccatc aacgaagaag caatgaaaat agccattgaa actaaccata
     3001 atgtcattcc cgttccagat cctggaattg cctgaggcaa ggcacttaat ggcggataaa
     3061 ttgtccaacc tgcagatgct ggtccagatt caacaaacaa cgaacaaacc atgattacac
     3121 ttgataaaaa gaataaccaa taagaaatca tgttcataaa accggaagcc atatcacgag
     3181 ctccaatttg caatggaata agtaaattac tgaacgtacc gcttaatcca gctgttaata
     3241 cgaaaaatac cataattgtt ccgtgaatgg tcacaagagc taaatagata tcatttcgca
     3301 ttacgccatc cggtgcaaaa gtatcaccta aaagtacatt gaatatctta aaagactctt
     3361 ctggccaagc taattgcatt ctgaacaata acgacatagc cacacctata atacccatta
     3421 tcaaacctgt aataaggtat tgtttggcaa tcattttatg gtcaatacta aagatgtact
     3481 ttgtgataaa agtatcttta tggtgatgtt cgtgttcgtg tccgtgatct tgttcgtttg
     3541 cgtgatcgtg tgctgacata tgacttaatt aatttatata ttttgctatt actttactac
     3601 ttgtactatt acagttgaat ctttaacaac agtagcagta tcaattgttg ctttatctgc
     3661 ttctacaggc tgttcttgtg ctttaatatt ttgaactaaa gttgtttgac cacctaacca
     3721 ttttttaaaa tcctcaggac tttccaccac aattttcatt tgcatattgt agtgagattt
     3781 tccacaaatt ttattacata gtaacaaata atcaaaggta taaggatcta atgcttctag
     3841 tcctttggca actaattctt tgcttttttc agttctgatt ttattaattg catctacttt
     3901 ttcaatcatt gctttcgaat ttctcatatc aacagtagtt actgttggag taaaagcaaa
     3961 ttgagttacc attccaggaa cacaattcat ttgtgctcta aaatgaggca tataagcaga
     4021 atgtaataca tcttgagaac gaattttaaa aacaactttg gtacctttag gtaaatgtaa
     4081 ttcagaagcc tgaatatcat cttgtgcatt gggatctgct aaatcaacac ctaacgtatt
     4141 tattccttct atgtaacgca catttgcttt acctaatacg ttatcttttc ctgcatagcg
     4201 aacttcccat ccaaattgtt tggcatatat ttctacaacc attggatttt ctgtttcttc
     4261 atctacaaac ataatatttg tccaagcata taatccgtaa agaattaaac cagccaatgt
     4321 aatagcagga ataatactcc aaattgcttc taatttattg ttatcggcaa agtataaagc
     4381 tgtattaccc tttttacctc tgtatttgaa tgcaaaataa tgcagtaacg cttgagtaac
     4441 cgtttgaaca aagaaaataa gaatcatcga gatccacatt aatctatcta cttcaacacc
     4501 atgttctgat gcaggattac tcattaaagg gaaatgacca aacttaacaa cacaatatat
     4561 agttgtcaaa taaatgaata ccaaaaaggc aaacattaaa tatccattta cactattatc
     4621 attatcattc gctacttgat aattttcggc attatcccct atttgagtta aatcaaatat
     4681 tttggttaat tgccaaatag caactgatac aaaaactaaa acaattataa ctaacaaact
     4741 tgtcatctgt ttaaatcttt taaatattaa taatgaaaat gtttactttc ttcaataaaa
     4801 ggatttcttt taggaaccaa tggggctttt gttaaagcag tgaaaactac taaaaggaat
     4861 aatccaaaga aaaacaataa agaacttatt tccccaacac cgataaacca ttggtcacca
     4921 acggtcgccg gcataatcat agtgaaaaaa tctatataat gtccaaataa aattaccaca
     4981 cctcccatta cgattatcca tgtgattctt ttgaaatctg tattaatcaa tatcaagaat
     5041 ggaaaaataa agtttaatgc taacattccg aagaatggta aattatattg ttcaattctg
     5101 gtaacaaaat aagttacttc ttccggaata tttgaatacc aaatcaacat aaactgagaa
     5161 aaccataaat atgtccagaa aatactaata ccaaacataa atttagctaa atcgtgaata
     5221 tgacttgtat ttacatgttc taaatatcct tttgatttta agtataaggt aactaaagcg
     5281 attacagtta ttccacttac gaagaaagaa gcaaaaacat accatccaaa taaagtagaa
     5341 aaccaatgtg gatcaacaga cataatccaa tcccatgaca taattgattc cgtaacaata
     5401 aagaaaacta aaaatgctgc tgacattttg aaatttttct tgtaaaatgt gttatcagat
     5461 gcctcatctt gtgctaaaca attttttctt gaaaaataac ggtataaatt ccaaccgatt
     5521 aagaaaatag ccgctctgat taaccaaaaa tactcattta aataacctga tttatttgca
     5581 attaatttat cgtattttgg attagcagga tctaataaat catgatccat ccatgcaaat
     5641 aaatgattgt aatgaaatgc tgataataat aataaaacga aaaaaattac tgaccctacc
     5701 ggtaaatatg ctgtaattcc ctgcattact ctaaataata taggtgacca tccggcctga
     5761 gctacatttt gaataccata aaatgctaat gctcctagtg aaattaacat aaagaataag
     5821 gctgcaacat atatagcggc ccatggcttg ttttccaatt gatgtaaaac atgtttcaaa
     5881 tgttcttggt gttctgcatc agcttttgca tcttttccat gtgcttcatg tgcagttgct
     5941 ccatgagcat ttccatgttc accatgagca tcggcttcaa gaattttttc tacctctgcg
     6001 gttgttttag gagcagaaat aaaactatat ccaattccca ataacccaac tagcattagg
     6061 acaatggaaa aaacttttaa tttacttgaa agggtataca tatctataca atctagttgt
     6121 caataattat aatttgcttt ttaactgaag aacataatcg gttactaacc aacgctcgtg
     6181 ttgagataat tgattagcat gagaacccat agcgtttaaa ccatatgtaa ttacgtgaaa
     6241 tacactgcct tcagttattg gtctatcagc ataacttgga acgcctaaga atttttctct
     6301 ttccactaat ttacctttac catttccttg ttcaccatga catatagaac agtaaataac
     6361 atataggtct ttcccttttg tagcttcttt ttcagaaatg gaatctatgg gtgattttaa
     6421 attagctttt gctaattcat agcctgcagt cgtattttca tactcataaa tttcaaatcc
     6481 tcttttgatt gttccggttg gcggctcttg agcagtttgc ccttgtaatt gatttttccc
     6541 attgaatgct tcagattctg cataggtttc atagcctaca gactggtaca tattagggaa
     6601 aaattgataa tttggactct tactgtcaaa gcaagatgta accgataagg ctaaaccaac
     6661 taatagggtt attttaaata agcttttcat aaaactatat taatgctttt ctacaacttt
     6721 taattcaatt gcaccagttc cttttagaaa agatgctaat tcatcttcat tattatggat
     6781 tgaaacttcc atgacaaaat gatcatctgt tgttctcaca tcaggatttt cagcttgttt
     6841 gaaaggccat aatttacttc tcatataaaa agtaattacc attaagtgag cagcaaagaa
     6901 aaccgttaat tcaaacataa ttggcacgaa agccggcatg ttttcaatat aactaaaact
     6961 aggttttccc ccaatatctt gtggccaatc ttgaatcatt atatagttca tcatccaaat
     7021 tgaaaaagac aaccccacta ctccatacat gaaggaagct attgcaattc ttgttggagc
     7081 taatcccatc gccttgtcca aaccatgaac aggaaaagga gtataaacct cttcaatatg
     7141 atgatgtgct ttacgcgttt ctttcacggc atccatcagg atgtcatcat cattatatag
     7201 tgcttgtata actttagtac tcataatatc ttactactaa ttatcttaat gattgtgatg
     7261 tccttcttct tctctttttt tcttataaaa ctcacctgaa gatttcaaga ttgtttttac
     7321 ttcagcttga gcaatcacag ggaaagatct agcatataaa agaaataata cgaaaaagaa
     7381 tcctatagtt ccaatataaa ttccaatatc tacgaaggtt ggagagaaca tcgtccaaga
     7441 tgatggaagg taatctctat gcaatgatgt tacgataata acgaaacgct caaaccacat
     7501 accgatgttt acaacaatgg aaataacaaa agaaaacatg atacttgttc ttaatttttt
     7561 aaaccacatg aactgtggag agaaaacgtt acaagtcatc attgcccaat aagcccatgc
     7621 ataaggtccg gttgctctgt ttaagaaagc atattgttca tattcaactc ctgaatacca
     7681 agccacaaat agctctgtaa tgtaggcaac acccacaata gaacctgtaa tcatgattac
     7741 aatattcatt aattcaatat gttgaatcgt gatgtaagcc tcaagattcg atacttttct
     7801 catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntctctca acatggcaaa
     7921 gtcaggaagt aaaccagtcc accagaaaac taaggatact gataaatacg tagaaattgc
     7981 aaatacgtcc caaagcaatg gtgagttaaa gtttacccac aacgaaccaa attggtttgg
     8041 aattggtaac acccaatatc ctaaccaagg tcgacccatg tggataattg gaaataaacc
     8101 tgcttgcatt actgagaaaa tagtcatcgc ttctgcagaa cggttaatgg ccattctcca
     8161 tttttgacgg aataacaaaa gtaccgctga aatcaatgtt ccggcatgac cgataccaac
     8221 ccaccaaacg aagtttgtga tatcccaagc ccaaccaact gttttgttta atccccatgt
     8281 tccgataccg gtagagatgg tgtaaatcat acaaccaact ccccaaagga aagcggttaa
     8341 tgcgattgaa aagacaatcc accattgttt atttgctctt ccttcaacag gagcagcgac
     8401 atctactgtt atatcgtgat aagatttatc accaataact aaaggttttc taatgggtgc
     8461 ttcgtagtga gacgacataa tcctttatag tatttcttat taatttaatt taattacgta
     8521 tttctaactt taacgtgata gaatacattt ggttttgttc ctacatgctc taacaaatga
     8581 tacatacgat catcttcaac caatttggct acttcacttt ctttattatt aacatcccca
     8641 aaagcaagag aaccggttga acaagcacta gaacaagctg tttggaattc gtcagcattt
     8701 acttgacgtc cttctttttt cgcttttaat attgttgctt gtgtcatttg aatacacatc
     8761 gaacattttt ccattacccc tcttgaacga acattaacat ccggattcaa taccatgcga
     8821 cctaaatcat cattcatatg gaaatcgaat tcattgtttt tgttgtataa gaaccagttg
     8881 aaacgacgta ctttatacgg acagttattg gcacaataac gagtacctac acatctgtta
     8941 taagccatat gattttgacc ttgacgacta tgagaagtgg cagcaaccgg acaaactgtt
     9001 tcacatggtg cgtgattaca atgttgacac attacaggtt ggaaagctac ttgagggtta
     9061 tccgcaggat gttctaattc tccaaatccg cttaaagaac ctttctctcc tgttaaacca
     9121 tcaaactcat cttttttctt attatcaccg gcaaaagtat cttcagaaga ataatatcta
     9181 tctatacgca accaatgcat atctctactt cttcttactt cagttttacc aactacaggt
     9241 acattatttt ctgcgtgaca ggcaataaca catgctccac aaccagtaca tgaatttaaa
     9301 tcaattgata aattgaaatg gtgtccaaca cttctgtcaa aactatccca taaatcaact
     9361 tttgtagctt caattggctt gtggtccata gataccattg gcggtggatt ccatactttc
     9421 gcatcttttt ctgcattaaa tacagtcaaa gtagtttctt taataatgtc tccacgaccc
     9481 attaatgttt tttgcaactg aacacaagca aattcgtgtt caccactttc caaagcaatc
     9541 gttgccgatt gagttgcgtt gaatccctta tataaagtgt aagcgttaac acctacttgc
     9601 attgcttctt ttaatgccgc ttttttaccg taacctagtg ctaatccaac ggttcctttt
     9661 gcttgacccg gttggataat tacaggaact ttttcaagtt ttgtaccacc tactgttact
     9721 gtaacataac ttccgttcaa tccgccattg gcaacattat gattttttac accatatttc
     9781 tcagcatctg cttttgaaat ggttaagtaa ttatcccatg aagctctagt aattggatct
     9841 ggaaattcct gtaaccatgg gttgttggct tgttggccat cacccatacc agttttggta
     9901 tataaattta attctaagcc actacttggt tttgcttgag ctaatgtagc tgctgctgct
     9961 gaaaaatcag ctgatgaagc tacatttgaa gaaacagttg ctgaaacaaa aattccgtca
    10021 tgaactgctt gattccaagt ttttccaact aaaactcctg tccatgattc ttttaagaaa
    10081 tcataatagg ttttatcact tccgttccaa actaataaat tgtcttgtaa ctgacgtgta
    10141 ttaaataacg gacgaatagt tggctgtgtt aaactgtaag tgcctttgat aatttgcaca
    10201 tcaccccaac tttctaaata atgaggagct ggagctgcta tatttgttaa agaagccgtt
    10261 tcgtcttctt ttaaagaaat agccacagaa agttttgttt ttttcaatcc gtcagcgaat
    10321 gctttacctt ctgctaaaga atataccgga ttcactccac tcataataag cgtgtgcact
    10381 tttccaccat ttaaatccgc cactaactgt gcaactgctt ggttagaacc ttttctgatt
    10441 tgacgggttt gatttggatt gaatgcttca ctatttaatt tagcattaat tgctaaaacc
    10501 aatagttgag catttttatc atcaattccg gaaactaaaa ctcctttaga tcctgattga
    10561 tttaattgtt ctgcaatttt tacaatttca gcttcattag ctaattttcc aacagcaaca
    10621 gaagttcctg taactatact gaaaatttta acaagagctt gtttttgttc tgcaacagac
    10681 attggaattc ttttgtcggc agctgcaccg gatagtgtca tgtttgcttc taattgaata
    10741 tgcttagaca tttttccttg tttaggaact cttccttgtg catatccttt gtcgtatccg
    10801 ccaccttgcc aatctcctaa gaaatctgct ccaacagaaa ctattgttga tgctttttca
    10861 aattcatatt cggctaaagc tctttctcca taaactgctt gaaatgcatc taaagcttct
    10921 gaagaagaaa ctgcatcata aataacatgt tttgcagttg ggttattaac caaaaactca
    10981 cttactaatt tatctgttga agggctcgct aaagtacctg ttaaaaggac tacctgagct
    11041 cctgttgctt ttgcatcagt taaactagct tttacttttg catcaacttc tgaccaagta
    11101 gcatcttttt gattcacttt aggttgtttc aaacgcatgt tatcatacat cgacaaaacc
    11161 gaagcatgaa cacgagcatt tgcagtaaat tttgcacctt caattgtgtt attttctatt
    11221 ttgattggac gaccttcacg cgtttttact aaaagattag caaaatcgaa accgtcgaac
    11281 atggaagtgg cataataatc agcaacaccg ggaataattt gctccggttg cacaacataa
    11341 ggaatagatt tgattaccgg tccttcacat gcagccaatg aagcggcggc agtgctaaaa
    11401 ccaacgtact ttaagaaatc acgtctagaa gtagaagaat ttgacaaact gtctttatct
    11461 cctaagaact catccgtagg gataggttcg atgaattcgt tatttcttaa cgtctcaaca
    11521 atagaactat tttcgtttag ctcttcaaca cttttccagt attttttgtt tgatgacata
    11581 ctgtatatat attagcttct taataaatta attaatagtg acattttcca cattctaaac
    11641 ctcccatatc ggcagcagtc aatttatctt tgccgtattt tttagaaagt tc
//
LOCUS       DAOTCW010000066        11630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3548_length_11630_cov_45.533823, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000066 DAOTCW010000000
VERSION     DAOTCW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11630
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3548_length_11630_cov_45.533823"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1085
                     /locus_tag="P5335_06830"
     CDS             81..1085
                     /locus_tag="P5335_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRZ74626.1"
                     /translation="MNLELKKRYFLFVLVVTQFAYSQEGIAVYSDYLTDNYYLIHPSM
                     AGAANCGKVRLTARGQWFGQDDAPSLSTLSFNTSVGERSGVGAILFSDKNGYHSQKGV
                     KLTYAHHIMFSRSELDLNQLSFGLSGALIQSQLDETSFLDNQPFDPNIGGITQKDSYF
                     NLDLGASYNYLEFYTHFTVKNLLGTVRRELYTDVESDNLRKYLISAGYVFGNSEQLQW
                     EPSVLFQMVEQTKEKSIDLNLKVYKNLDFGKMWGGLSYRRSFDGAEYLDGGNVGEQRL
                     QYFTPILGVNYNQFMFAYTYSYLSGNVKFDSAGFHQITLGINLFCKREKYQCNCPAIN
                     "
     gene            1157..1675
                     /locus_tag="P5335_06835"
     CDS             1157..1675
                     /locus_tag="P5335_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRZ74627.1"
                     /translation="MIIKTVNGKSPLIPEDCYVAENATIVGDVQFGQSCSVWFNAVVR
                     GDVHFIKIGNKVNIQDGAIIHCTYKKHPTIIGNNVSIGHNAIVHGCTIHDNVLIGMGA
                     IVMDNCVIESNSIIAAGAVVTQNTKVESGSIYAGVPAKKVKDINQSDFAGEIERISNN
                     YIMYSSWFKEEN"
     gene            complement(1672..2448)
                     /gene="murI"
                     /locus_tag="P5335_06840"
     CDS             complement(1672..2448)
                     /gene="murI"
                     /locus_tag="P5335_06840"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRZ74628.1"
                     /translation="MVNNNPIGLFDSGVGGTSIWKEIHALLPHENTIYLADSKNAPYG
                     QKSKEEIIALSKKNTEFLLNKNAKIIVVACNTATTNAIKELRASYDVPFIGIEPAIKP
                     AANNSETHTIGILATKGTLSSELFNQTVLNYPNTKIIEQIGFGLVSLIEEGNIESKEM
                     TQLLHDYLNPMVKENIDYLVLGCSHYPYLIPQIKKILPNHIKIIDSGEAVARQTKNIL
                     SDKVGLSDTKKSKQLFYTNSNPIVLKNLIGGKHTIIEENF"
     gene            complement(2530..3030)
                     /locus_tag="P5335_06845"
     CDS             complement(2530..3030)
                     /locus_tag="P5335_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRZ74629.1"
                     /translation="MKQIKTLLIATFLFIGASQSISAQSKIAHVDTNELISKMPAMIE
                     AQKQLENLSKQYDAEFKTMADEYYAKLKKYDEESSTVTAKVNEDRAKEVQDMEKRIRD
                     YRETAQKELQKKESDLAKPLMDKIKASIAKVGKAKGFNYVIDVNSAIFADGTDLTSDV
                     KKDLGF"
     gene            complement(3049..4089)
                     /locus_tag="P5335_06850"
     CDS             complement(3049..4089)
                     /locus_tag="P5335_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRZ74630.1"
                     /translation="MKKLIFLLFLTLTSASIEAQSRGIRIGYIDMEYILQNVPDYTEA
                     KNQLEQKAQKWKLDIETKKNEINTLKEALNNEKVLLTKELIEEREEEITFLENELLAY
                     QQKRFGPTGDLVVQKAVLVKPIQDQVFTAVQDIAEVKKYDFILDKSSDLTILFAAKRH
                     DISDQVIRSLTRASKREQMSKKELKALEEKERIQDYAEDVNPDKEARKKILEDRQKQR
                     DSIIESRKSLAEQKKKDFEDKRKKLIEERAAKKNGTVSDKDKQVNVEEGSETTDKSAE
                     AVEEKSKSDETKSDAAETKKKAEEERQKKLDERKKQLEERKQKILADREAAKKAKEDK
                     QNENKEENKNNN"
     gene            complement(4130..6844)
                     /locus_tag="P5335_06855"
     CDS             complement(4130..6844)
                     /locus_tag="P5335_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRZ74631.1"
                     /translation="MKLLLVIKKENVGLEKQVNRLNKTVVLRNITSFFAILLLGSIFQ
                     VNAQNTTPYERGSQYILGDVQVTGKLSFNQQTVITFAGLEKGQKVIVPGEEISSAIKK
                     LGKLGLFSDIDVYINEIRGDSIFLELSLVELPKLSEVKIVGLKKNKNEELLKELALTK
                     GKVVNENLLTTSKNFIEKKFQKDGFYNTKVFATITPDTTNGNEVKMLINVDKGTKVKI
                     SSIAIEGNVTQTEKNVKSAMKNTKEQSFFRFWKSSKFIQQEYNEDLVNIVNKYKTVGY
                     RDARIVYDSVKYKKEDNTIAIKLKIEEGRKYYFGNIKFLGNTIYTDQGLNRVLGIKKG
                     ETYNGVLLQDRIANKKNPDAEDITNLYQNNGYLFSTINPVEVRTANDTIDFEIRIMEG
                     PLAYFNKITVVGNDKTNDHVIYRELRTKPGQKYSKDALIRTIREIGQLGFFDPEAIDP
                     KFKNVDASAGTVDIEYNLVEKGSSQIELQGGYGGGGFIGTLGLSFNNFSIRNVFNKEA
                     YKPLPMGDGQKMSLRLQGSTYFQTYSLTFSEPWFGGRKPMQFSTSLSYSKQFLNNYQT
                     NDVDKSRSFNITSIAVGTAKRLKVPDDFFLLSQGLSLQYYDLNNYSTSLFTFPNGSSR
                     NLSYFISLSRNNKGVNPIFPVYGSEFSISAKFSPPYSLFNNVDYKDLPNQEEYKVKTT
                     EVIPDPNTGDDMPIGTYLDDSGNPVENYQDAAVDQGKVDQKAFYWLEFYKIKLKGDWY
                     TKLAGKNQNALVLRTLGEFGYLGAYNQDRGVIPFERFFLGGDGLANYSLDGRDVIQLR
                     GYPNLSLSGTDGGVIYNKFSMELRYPLTLKSAASIYMLTFAEAGASFNSLQEYNPFVM
                     QRSAGFGLRVFMPAFGLLGIDFGYGFDAVPGQTQKNGWETHFIIGQQF"
     gene            complement(6777..7517)
                     /locus_tag="P5335_06860"
     CDS             complement(6777..7517)
                     /locus_tag="P5335_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRZ74632.1"
                     /translation="MTLEDQINKSNLPKHLAIIMDGNGRWAKQKGLLRALGHESGTKA
                     VRETVESCAKLGIQNLTLYAFSTENWNRPKLEVDTLMNLLIKSLKDELKTLQKNEIKL
                     NSIGNISLLPKSAQKELLEVINKTKENNRMTLTLALSYGAREELVSAIKAISDKVKNN
                     IISSESIDETIINQHLYTHNLPDVDLLIRTSGEHRISNFLLWQIAYAELYFTDVLWPD
                     FREENLYEAIISYQKRERRFGKTSEQIK"
     gene            complement(7520..8203)
                     /locus_tag="P5335_06865"
     CDS             complement(7520..8203)
                     /locus_tag="P5335_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRZ74633.1"
                     /translation="MNRLVFLLFFMVSSTVLKAQINEIGVFLGGSNYIGDVGPTNYIA
                     PNDIAIGFVYKWNRSTRHTYRFSYTYGQLSSNDADSDVPDRNLRGLSFENKVNEFSAG
                     LEFNFFDFDLHKLSSQFTPYVYTGISYFSYKELFYINNEVKEDYTEGALAIPIVLGVK
                     AKLNRRFIIGLEAGARYTFTDNLDGSNPKNENLNSLKFGNINSNDWYVFTGFTLTYTF
                     GENPCFCPQ"
     assembly_gap    8345..8444
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8445..9034)
                     /locus_tag="P5335_06870"
     CDS             complement(<8445..9034)
                     /locus_tag="P5335_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRZ74634.1"
                     /translation="MKIAIFGQYYQNDTRPIIKDIFVFFNSNHVELVIEESFLKILYE
                     EEIVKKEYKTFATYKDLNSSFDLLISIGGDGTILRAITYVRNSGIPILGVNAGRLGFL
                     AKVQKENIETFLQLILDKNYTVSKRTLLSIESNPKNDEIKEINFALNEISVSRKDTTS
                     MITVETYLNGEYLTSYWADGLIISTPTGSTGYSLSCG"
     gene            complement(9038..9697)
                     /locus_tag="P5335_06875"
     CDS             complement(9038..9697)
                     /locus_tag="P5335_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRZ74635.1"
                     /translation="MLEITNYINNDFKPFDSGEKINYVQDFFADLTFSHFPVVEESIY
                     IGSIAADDVVTFETQKSIKDYRFTLEGFFARTNMIWLDVLEVFAQNNTNLIPVLDDQN
                     KYVGYYELNDIVQFFAETPFLREQGGIIIVKKPLNDFSMSQITQIVETNNGKILGLFI
                     SEATTEFVQVTIKITLGGMNEIIQTFRRYNYEIISEHQEDTYLSNLKERSEYLDKYLN
                     I"
     gene            9801..10514
                     /locus_tag="P5335_06880"
     CDS             9801..10514
                     /locus_tag="P5335_06880"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRZ74636.1"
                     /translation="MTKLSVNINKIATLRNSRGGNVPNVVQVAIDVQKFGAHGVTIHP
                     RPDERHIRYQDARDLKAVVYTEFNIEGNPVQNFIDLVLEVNPTQVTLVPDADDALTSN
                     AGWNTIKNKDLLTEVIQEFKRNNIRTSLFVDPDLTMVEAAKITGTDRIELYTEHFAHQ
                     YGLGNHSGIQPYVEAAVLANQLGLGINAGHDLSLDNIGFFKQNIPGLLEVSIGHALIC
                     ESLYLGIENVVNMYLHKLK"
     gene            complement(10516..10995)
                     /locus_tag="P5335_06885"
     CDS             complement(10516..10995)
                     /locus_tag="P5335_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74637.1"
                     /translation="MNKFLSLLSFLFLLTSCQYFEKRVPSEEELLQQRLKEIDWKEVT
                     VYPSVAECDSLLDKEQKKECFFNFMTVLIQQKIAPDSINQKVKIIDTIQVKVSIYPNA
                     TISFEPLFPSDSLNYDKIKIDSLIKSRLTNFPKIEPAQKEGIPVKTQFILPVILKME"
     gene            11055..11555
                     /locus_tag="P5335_06890"
     CDS             11055..11555
                     /locus_tag="P5335_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRZ74638.1"
                     /translation="MEHILLLLGFVLMIVGVLGSFLPVLPGPPISWVGLLLLYLTKAV
                     PVSYTVLGITLVIAIVVGILDYIIPAKGTKRFGGSKYGIWGTNIGLVVGILAPIPLGF
                     IIGPFVGAFVGELINDSTDSKKAFKAATGSFIGFLASTFMKFLVSMIFLGLFLVKVWE
                     YRNAWF"
BASE COUNT         3614 a         1865 c         1850 g         4201 t
ORIGIN      
        1 actggtttac ggtggagtat gaagaaaacg gtgaatctaa aattttcaaa gcacattttt
       61 cattaaaaag ataatagccc atgaatcttg aacttaaaaa aaggtatttt ttatttgtat
      121 tagtagtaac acagtttgct tattcacaag aaggaatagc ggtttattct gattatctaa
      181 ccgataatta ttatttaatt catccatcaa tggctggtgc agcaaattgt ggaaaagtca
      241 gattaacggc aagaggacaa tggtttggtc aagatgatgc ccccagttta agcacactta
      301 gttttaatac aagtgttggt gaacgatcag gagttggagc tattttattt tcagataaaa
      361 acggttatca ttctcaaaag ggagtaaaac ttacttatgc tcaccacatt atgttttcta
      421 gaagtgaatt agatttaaat caattatcat ttggtcttag tggagcatta atacaaagtc
      481 agttggatga gacttctttc ttagataatc aaccatttga cccaaacatt ggaggcataa
      541 ctcaaaaaga ttcttatttt aatttagatt taggagcttc ttataattac ttagaatttt
      601 atacccattt tactgtaaaa aatttattag gaacagttag aagagaattg tatactgacg
      661 ttgaatctga taatctaaga aagtacttaa taagtgcagg gtatgttttt ggaaacagtg
      721 aacaattgca atgggaacct tccgttttat ttcaaatggt agagcaaaca aaagaaaaat
      781 caattgactt aaatttgaaa gtatataaaa atttagattt cggtaaaatg tggggcggat
      841 tatcttatcg aagaagtttt gatggggcag aatatttaga tggtggaaat gtaggagaac
      901 aacgattaca atattttaca ccaattctag gtgtaaatta taatcaattt atgtttgctt
      961 atacttactc ttatttaagt ggaaatgtaa aatttgatag tgccggtttt catcaaataa
     1021 cattaggaat aaatttattc tgtaaaagag agaagtatca gtgtaattgc cctgctatta
     1081 actaaattaa aaataattat aaatgaaaat ccctttaccg taaaaaagtt tggggatttt
     1141 ttttaactaa agtactatga taataaaaac agtaaacggt aaatcaccct taattccgga
     1201 agattgttac gtcgctgaaa atgctactat tgtgggagat gttcaatttg gacagtcttg
     1261 tagtgtatgg tttaacgcag tagttcgtgg cgatgtacat tttataaaaa ttggcaataa
     1321 agtgaacatt caagatggag caatcattca ttgcacttat aaaaagcatc ccacaattat
     1381 tggcaataat gtttcaattg gtcacaatgc aattgttcat ggctgcacta ttcatgataa
     1441 tgtattaatt ggaatgggag caatagtaat ggataattgt gtgattgaaa gcaattccat
     1501 catagctgcg ggtgctgttg taactcaaaa tactaaagta gaatcaggtt caatttatgc
     1561 aggtgtaccg gcaaaaaaag tgaaggatat caatcagtct gattttgcag gtgaaattga
     1621 acgcatatca aataattata tcatgtattc aagttggttc aaagaagaaa attaaaagtt
     1681 ttcttcaata attgtgtgtt ttccaccaat taaattttta agtactattg ggtttgaatt
     1741 cgtgtaaaat agttgtttac tttttttcgt atcgcttaaa cctactttat ctgaaaggat
     1801 gtttttggtt tgtcgggcta cagcttctcc ggaatcaatt attttgatat ggttgggcaa
     1861 tatttttttt atttgtggta ttaaataagg ataatgacta cagcctaata caagataatc
     1921 aatattttct ttaaccatag gattcaaata gtcatgaagc agttgagtca tttctttaga
     1981 ttcaatattt ccttcttcaa tcaacgaaac caaaccaaag cctatctgtt ctattatttt
     2041 tgtattggga taattaagca cggtttgatt aaataattca ctactcaaag tgccttttgt
     2101 agctaagata ccaatggtat gtgtttcaga attatttgct gcgggtttaa ttgccggttc
     2161 aatgcctatg aatggaacat cgtatgaggc tctcagttct ttaatagcat ttgtagttgc
     2221 agtattgcat gcgactacaa taattttggc atttttgttc aaaagaaatt cagtgttttt
     2281 tttacttaac gcaataattt cttcttttga tttctgacca taaggtgcat ttttactatc
     2341 cgcgagatag attgtgtttt catgaggaag cagtgcgtga atctctttcc aaatagaggt
     2401 tcctccaact cctgaatcaa aaaggcctat gggattatta ttaaccatgt atcaaaaata
     2461 aataaaaaac tgctcatatc ccgttatttt gggtgagcag tttttaatta tgtattttaa
     2521 gtttaagaat taaaaaccta aatctttttt tacatcagaa gttaagtctg ttccatcagc
     2581 aaaaatagct gaattcacat ctattacata gttgaaacct tttgctttac caacttttgc
     2641 aatcgaagct ttgattttat ccattaatgg tttagccaaa tcagattcct ttttttgtaa
     2701 ttctttttga gcagtttctc tgtagtcacg gattcttttt tccatgtctt gtacttcttt
     2761 tgctctgtct tcatttactt ttgctgtaac agtagatgat tcctcgtcat attttttcaa
     2821 tttagcataa tattcatctg ccattgtttt aaattcagca tcatattgtt tactcaaatt
     2881 ttctaattgt ttttgagctt caatcattgc cggcattttg cttatcaatt cattagtatc
     2941 aacgtgagct atttttgatt gagctgaaat agattggctg gcaccaataa ataaaaaagt
     3001 agcaattaat aaagtcttaa tttgtttcat catttctagt tattataatt aattattgtt
     3061 tttgttttct tctttgtttt cgttttgttt gtcttctttt gcttttttag cagcttcacg
     3121 gtcggctaat attttttgtt ttctttcttc aagctgtttt ttgcgttcgt ctaatttttt
     3181 ctgtctttct tcttccgctt ttttctttgt ctcagcggca tctgattttg tctcgtcaga
     3241 cttgcttttt tcttcaacgg cttccgcaga tttatcagtt gtttcagaac cttcttcaac
     3301 gtttacttgt ttgtctttat cagaaactgt gccattcttt tttgctgccc gttcttcaat
     3361 tagttttttc cgtttatcct caaaatcttt tttcttttgt tctgcgagtg attttcgaga
     3421 ttcgatgata ctatcacgtt gtttttgtct gtcttcaaga atcttttttc gagcttcttt
     3481 atcaggatta acatcttcgg cataatcttg aatgcgttct ttttcttcta atgctttgag
     3541 ttctttttta ctcatttgct ctcttttaga ggctctggtt aaagatcgaa taacctggtc
     3601 actgatatca tgtcttttag cagcaaaaag gatagttaaa tcagaagatt tgtctaatat
     3661 aaaatcatat ttttttactt cagcaatgtc ttgcacagca gtaaaaacct gatcttgtat
     3721 gggttttact agaacagctt tttgaacaac caaatcgccg gttggtccaa atcttttttg
     3781 ttgataagct aaaagttcgt tttcaagaaa ggtaatttct tcttctcttt cttcgattaa
     3841 ttctttggtt aaaagtactt tttcgttgtt taaagcctct tttaaggtat tgatttcatt
     3901 ttttttagtc tcaatatcta atttccactt ttgagctttt tgttctaatt gatttttagc
     3961 ttcagtatag tcaggaacat tttgaagtat gtattccata tcaatataac caattctaat
     4021 acctcttgac tgtgcttcga tagaggcact tgtaagagtt aaaaacagta aaaaaattaa
     4081 tttcttcata ttttataaat tataatgtaa atataacaga aattaacaat taaaattgtt
     4141 gtccaatgat aaaatgtgtt tcccagccat ttttttgtgt ttgtcctggc acagcatcaa
     4201 aaccatagcc gaaatcaatt cctaataatc caaaggctgg catgaagact cttaaaccaa
     4261 aaccggctga gcgttgcatc acaaatggat tgtattcctg taatgaatta aatgaagctc
     4321 ctgcttctgc aaaagtaagc atatagatgg aggctgcgga ttttagtgtt aatggatatc
     4381 gtaattccat tgaaaattta ttgtaaatta ctccaccatc tgttcctgaa agcgataaat
     4441 tcggatagcc tcttaattga ataacatctc taccatctaa tgaataattc gctaaaccat
     4501 cacctcctaa aaagaaacgc tcaaatggaa tcacacctct gtcttggttg taagctccca
     4561 aataaccaaa ttctcctaat gttcttaata ctagagcatt ttgatttttc ccggcaagtt
     4621 tagtatacca gtctcctttt aatttaattt tataaaattc taaccaatag aaagcttttt
     4681 gatccacttt tccttggtca acagcagcat cttggtaatt ttcaaccgga ttgccagaat
     4741 catcaagata ggttccaatc ggcatatcat cccctgtatt tgggtctgga attacttctg
     4801 tcgtttttac tttatattct tcctggttgg gtaaatcttt gtaatccaca ttattgaata
     4861 acgaataggg tggagagaat ttagccgata ttgaaaactc agaaccataa acagggaaaa
     4921 ttggattcac tcctttattg tttctgctta aggaaataaa ataggataga tttcgggaag
     4981 aaccgttagg aaaagtaaac aatgaagtag aataattatt caaatcataa tattgtaaac
     5041 ttaatccttg tgaaagtaaa aagaaatcat ccggcacttt caatcttttt gcagtcccaa
     5101 cagcaataga ggtgatatta aaacttctac ttttatctac atcatttgtt tgataattat
     5161 ttaaaaactg tttactgtag gataaggaag tagaaaactg cattggtttt cttccaccaa
     5221 accaaggttc agaaaaagtt aaactgtagg tttgaaaata ggtacttcct tgtaatcgta
     5281 atgacatttt ttgcccgtca cccattggta aaggtttata ggcttctttg ttaaaaacat
     5341 ttcgaataga aaagttgtta aaggaaagac ctaaagttcc aatgaagccg cctccaccat
     5401 aacctccttg aagctcaatt tgactggagc ctttttcaac taagttatat tcaatatcca
     5461 ccgttcctgc tgaggcatct acatttttaa acttgggatc aatagcttcc gggtcaaaaa
     5521 agcccaactg tccaatttcc cgaatggttc taattaaagc atctttacta tatttctgac
     5581 ctggttttgt gcgtaattct cgataaatta catggtcatt cgttttgtcg tttccaacaa
     5641 cggtaatttt attaaaatag gctagtgggc cttccataat tctgatttca aaatcaatag
     5701 tgtcattagc tgtacgaact tctaccggat ttatagtgga aaaaagataa ccattgtttt
     5761 ggtataagtt tgtaatgtct tctgcatcag gattcttttt gtttgcaatt cgatcctgaa
     5821 gcaatacgcc attataagtt tcaccttttt taattcctaa aacacggttt aagccttggt
     5881 cggtgtaaat ggtgtttcct aaaaacttaa tatttccaaa gtaatatttc ctaccttctt
     5941 ctatttttaa tttgatggca atagtattgt cttctttttt gtatttaaca ctatcataaa
     6001 caatgcgagc atcccgataa ccaacagttt tgtacttgtt aacaatgttt actaaatctt
     6061 cgttgtattc ctgttgaata aatttagatg atttccaaaa acgaaaaaaa gactgctctt
     6121 tagtgttttt catcgcactc tttacgtttt tttcggtctg agttacattg ccttcaatgg
     6181 cgatactgga aatttttact tttgtgcctt tgtccacatt aatgagcatt tttacttcat
     6241 ttccattggt agtgtctgga gttatggtag cgaaaacttt tgtattgtaa aaaccgtctt
     6301 tttggaattt cttctcaata aagtttttag aagtagtcag taaattttca tttacaactt
     6361 tgcctttagt taaagccaac tcttttaaaa gttcctcatt tttattcttt tttagaccaa
     6421 caatttttac ttcacttaat ttgggaagtt cgaccaagct tagttctaaa aaaatactgt
     6481 ctcctctaat ttcattaatg taaacatcaa tatcactgaa aagccctaat ttaccaagtt
     6541 ttttaatggc tgaactgatt tcttcaccgg ggacaataac tttctgccct ttttccaatc
     6601 cggcaaaagt gataactgtt tgctgattaa agcttaattt accggttact tgaacatctc
     6661 ctagaatata ctgggacccc ctttcataag gagtagtgtt ttgtgcattt acctgaaaaa
     6721 tacttcctaa tagaagtatt gcaaaaaagg aagtaatatt tctcaatact actgttttat
     6781 ttaatctgtt cacttgtttt tccaaaccta cgttctcttt tttgataact aataatagct
     6841 tcatataaat tttcttctct aaaatcaggc cataaaacat ctgtaaaata taattcggca
     6901 taggctattt gccatagtaa aaaattactt attcgatgtt ctccactggt tctaatcaat
     6961 aaatcaacat ccggtaaatt gtgtgtgtaa agatgttgat taataatcgt ttcatcaata
     7021 gattctgacg aaattatatt atttttaact ttatcactga tcgcttttat agctgaaacc
     7081 aattcctctc tcgctccata gcttaaagct aaagttaaag tcatacggtt attttctttg
     7141 gttttgttaa tgacttctaa aagttctttc tgtgccgact tcggtaagag cgatatattg
     7201 ccaatagaat ttaacttaat ttcatttttt tgaagtgttt tcaattcatc cttcaatgat
     7261 ttaattaata aattcattaa agtatccacc tctaatttag ggcgattcca attttccgtt
     7321 gaaaaagcat ataaagtcaa attttgaata cctaatttag cacaagattc cactgtttcc
     7381 cgaacagctt ttgtcccact ttcatgacct aaagcccgaa gtagaccttt ttgttttgcc
     7441 caacgaccat ttccatccat gatgatggcc agatgttttg gtaagttact tttatttatt
     7501 tgatcttcta aggtcatttt tattgcggac aaaaacaagg gttttcccca aaagtataag
     7561 ttaatgtaaa tccggtaaat acataccaat cgttgctgtt tatatttcca aatttcaatg
     7621 agttcaaatt ttcatttttc ggattgctcc catctaaatt atctgtaaag gtatatcggg
     7681 caccggcttc taaaccaatt ataaatcttc tgttcaattt tgcttttaca cctaatacga
     7741 taggaattgc taatgcacct tctgtataat cttctttcac ttcattattg atataaaaca
     7801 attctttgta tgaaaaatag cttataccgg tgtaaacata tggtgtaaat tgagagctta
     7861 atttatgcaa atcaaaatca aaaaaattaa attccaatcc ggcagaaaac tcatttactt
     7921 tattttcaaa acttaaccct ctcaaatttc tatccggaac atcagaatcg gcatcgtttg
     7981 acgataattg gccataagta taggaaaaac gatacgtatg gcgggtactt cggttccatt
     8041 tatacacaaa tcctattgct atgtcgttag gagcaatata atttgtggga ccaacatcac
     8101 caatgtaatt actgccacct aaaaaaacac caatttcatt aatttgggct ttaagtacag
     8161 tacttgatac cataaaaaac aataaaaaca ctaatctatt catccgtttt gaaatagcgt
     8221 gcaaatataa caaatatgac taggtaaaag tcaactattt gcttatttat cacaaattaa
     8281 tttcaaaaaa aacggaaata aaagggtagt agtatagaag agaagatata ttttgattat
     8341 taacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccacag cttaacgaat
     8461 atccggtaga gcctgttgga gtggagataa ttaatccatc agcccaatat gaagtcaagt
     8521 attcgccgtt taaataagtt tccaccgtaa tcatcgatgt cgtatctttt ctactgacgg
     8581 aaatttcatt gagagcaaaa tttatttctt taatttcatc atttttaggg ttgctttcta
     8641 tgcttaaaag ggttctttta gagaccgtat aatttttatc taaaattaat tgcaaaaaag
     8701 tttcgatatt ttctttttga actttagcta aaaaccctaa ccttcccgca ttaacaccta
     8761 atatgggaat acctgaattt cgaacgtagg taattgctct caaaattgta ccatcaccac
     8821 caatactgat taaaaggtca aaactgctgt ttaaatcttt ataagtggca aaagttttat
     8881 actctttttt aactatttct tcttcataca atattttcag aaaagattct tctatgacta
     8941 attcaacatg gttactatta aaaaaaacga aaatgtcttt aataattggg cgggtatcgt
     9001 tttgataata ttgtccaaaa atagcaatct tcatagttta gatattaaga tatttgtcta
     9061 aatattctga acgttctttt aaattactca aataggtatc ttcctgatgc tcagaaatga
     9121 tttcataatt ataccttcta aaagtttgaa tgatttcatt cattccgccc aaagtgattt
     9181 tgatagtcac ctgaacaaat tcggtagtag cttcagaaat aaaaaggcct aatatttttc
     9241 cgttgtttgt ttctacaatt tgagttattt gactcatgga aaaatcattt aatggttttt
     9301 ttacaatgat aatgcctcct tgctctctta aaaagggagt ttcggcaaaa aactgaacaa
     9361 tgtcattgag ttcataataa ccaacatatt tattttgatc gtctaaaacc ggaattaaat
     9421 tagtgttatt ttgagcaaaa acttctaaca cgtctaacca tatcatatta gttcttgcaa
     9481 aaaaaccttc taaagtgaat cgataatctt taattgattt ttgtgtttca aaagtaacca
     9541 catcatcggc cgcaatactt ccaatataaa tactttcctc cacaacaggg aaatgagaaa
     9601 aagtcaagtc agcaaaaaaa tcctgaacat aatttatttt ctccccactg tcaaagggtt
     9661 tgaagtcgtt gttgatataa tttgttattt ctaacatagc gatttaattt cgttgcaaaa
     9721 taatcaaaaa atacgattat tgagcctgtt taactttgta tttttgtatg ctgtttctaa
     9781 taaaatagtt tcaaaagggt atgacaaagt taagcgtaaa tattaataaa attgctacat
     9841 taagaaattc aagaggtgga aatgtgccaa atgtggtgca agtggctata gatgttcaaa
     9901 aatttggagc tcatggagta actatacacc cacgtccgga tgagcgacat attcgatatc
     9961 aagatgcaag ggatttaaaa gcggtggttt ataccgaatt taatattgaa ggaaatcctg
    10021 ttcaaaattt cattgattta gtgttggaag taaatccaac acaagtaacc ttggtgccgg
    10081 atgccgatga tgctttaact tctaatgcgg gttggaatac gattaaaaac aaagatttat
    10141 taacggaagt aattcaggaa tttaaaagaa ataacatacg aacatcgctt tttgttgatc
    10201 cggatttaac gatggtggaa gcagcaaaaa ttaccggaac ggatcgaatt gaattatata
    10261 ccgaacattt tgctcatcaa tatggtttgg gaaatcatag cggaattcag ccttatgtag
    10321 aagcagctgt gttggccaat cagttagggt taggaatcaa tgccggacac gatttaagtt
    10381 tggataatat tggttttttt aaacaaaata tacccggatt attagaggtt tccatcggtc
    10441 atgccttgat ttgtgaatca ctttatttag ggattgaaaa tgttgtaaat atgtatttac
    10501 acaaattaaa ataagctatt ccatttttag gattaccggc agaataaatt gtgtttttac
    10561 gggaattcct tctttttgag caggttctat ttttggaaaa ttcgtcaaac gggatttaat
    10621 aagactatca atttttattt tgtcataatt caaactatcc gaagggaaaa gcggttcaaa
    10681 agaaatggta gcatttggat aaatggatac tttcacttga atggtatcaa ttatttttac
    10741 cttttgattg atggaatccg gagcaatttt ttgctgaata agaacagtca taaaattaaa
    10801 aaaacattct tttttttgtt ctttgtctaa aagactgtca cattccgcca ctgagggata
    10861 aacagtcact tccttccaat caatttcttt caatcgttgt tgcaacaatt cttcttcaga
    10921 aggcacacgc ttttcgaagt attgacagga agtcaataaa aataaaaaag aaagtaaaga
    10981 aagaaattta ttcatttttc gtttataatc aaacaaaagg tatttttgat attccaaaag
    11041 taagaaaata atttatggaa cacatacttc ttttattagg ttttgtatta atgattgttg
    11101 gtgtccttgg aagtttcctt cctgttttgc ctggaccgcc tataagttgg gtgggtttgc
    11161 tgttattgta tttaacaaaa gccgttcctg tgagctatac agttttggga attacgttgg
    11221 taattgccat tgtcgttgga attctggatt acatcatccc tgcaaaagga accaagcgat
    11281 ttggcggaag caaatacggc atttggggaa ctaacatcgg gttagtggtt ggcattttag
    11341 cacccattcc actcggattc attattggtc catttgtggg agcatttgtg ggcgaactaa
    11401 ttaacgattc tacagattct aaaaaagcat ttaaagccgc cacaggatca tttattggtt
    11461 ttttagcttc cacatttatg aagtttttgg tttctatgat ttttcttggt ttgtttttag
    11521 tgaaagtttg ggaatatcga aatgcttggt tttaaataaa aaaggaccga aattatcttc
    11581 cggtcctttt aaagtaatgt aacttttcgt ttattgtttt acaaatttct
//
LOCUS       DAOTCW010000067        11308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3702_length_11308_cov_43.618502, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000067 DAOTCW010000000
VERSION     DAOTCW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11308
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3702_length_11308_cov_43.618502"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /locus_tag="P5335_06895"
     CDS             complement(<1..2071)
                     /locus_tag="P5335_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRZ74639.1"
                     /translation="MKKFYFLIILLFQSGLSLLFAQEGITQHTVLKGETVISIAQKYK
                     VTPNDLYKLNPELANGIKENEVINIPVSLVTKLQKTEPKSENNASNSNNDVIIYTVNP
                     GETKFGLAKRFGISTSQLENQNPHIVNGLQSGHKLEIRGGKEVYVSKESKNVNQFTDF
                     IIYVVLPKETLYGISKRNGLTVDELTEANKDFLNGVLKSGQTLRIPVKKNNSSISENS
                     NYHLVLAGETKYGLSKRYGISIEELEAKNPQIIKMLQTGQRIVVSGGVSSNAVSKQEV
                     PKSEPVLVEVPKVEQENVKSKDNPVKVEVPQSTDSGWVDYEVQPKQTLFGLSKMTGMS
                     QDKLIEVNPNLAEGVKIGMIIKIPSDKITTELAAAYKVPESKTVVPDVEITQNKVESK
                     VENKKEQIGLLKTLNTMEKKEVVMLLPFSTEKYEDFIKNQTLSTNQSTEFDFYAGATF
                     AVDSLKRMNVKVDVKTIELEFNKEMKAELVSLKKNKVENSNAILCFSSKGSTEKIGDF
                     ASKNNIPFIVNRQEEGSKNYPSTFVSIPSKNDLAQMMLNYIAEKNGNLIVVSDAINAL
                     NEDFILQNFPKARFVKLSSKGVLESESLTNELILNRKNYVVLNTDKTGLILNTTTILL
                     KESKDYQIQLALLQPKEYISGEGLSDMRFKALKMLYPSYSKRNNQEEINRFKIDFKRK
                     YNFEPNPE"
     gene            complement(2188..3462)
                     /gene="glyA"
                     /locus_tag="P5335_06900"
     CDS             complement(2188..3462)
                     /gene="glyA"
                     /locus_tag="P5335_06900"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRZ74640.1"
                     /translation="MQRDEQIFDLILDEQDRQIHGLELIASENFVSDQVMEAAGSVLT
                     NKYAEGYPGKRYYGGCEVVDQVEQIAIDRAKALFGADYANVQPHSGSQANTAVFFACL
                     KPGDKILGFDLSHGGHLTHGSPVNFSGRLYNPVFYGVEKETGRLNYDKIQEIATKENP
                     KMIIAGASAYSRDMDFKRFREIADSVGAILLADISHPAGLIAKGLLNDPVPHCHIITT
                     TTHKTLRGPRGGMILMGKDFENPFGIKTPKGEIRLMSSLLDAAVFPGNQGGPLEHIIA
                     AKAVAFGEALSDEFFRYSLQVQKNAKAMAEAFVKRGYDLISGGTDNHMMLIDLRNKNI
                     TGKEAENALVKAEITVNKNMVPFDDKSPFVTSGIRVGTPAITTRGLVEADMETVVALI
                     DRVIMNFTNEEIIEEVANEVNDFMGERAIFVF"
     gene            3573..4853
                     /gene="fahA"
                     /locus_tag="P5335_06905"
     CDS             3573..4853
                     /gene="fahA"
                     /locus_tag="P5335_06905"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HRZ74641.1"
                     /translation="MPITANNPNRKSWIEVPIDSDFPIQNIPFGVFLTKDDIVTIGTR
                     IGDCAIDLGAMQQLNYFEGIDLTDDMFMQDTLNDFISDGKKTWRLVRNRIGDLFDSNN
                     PKLRDNKEHRDIIIFKMDDVEMQLPVLIGDYTDFYSSKEHATNVGKMFRDPENALLPN
                     WLHIPVGYHGRSSTIIPSGIPVHRPMGQTLPNGDTQPVFGPSRLVDFELEMAFITTDA
                     NIMGENIPVSEAEDYIFGMVLLNDWSARDIQKWEYVPLGPFLAKNFASSISPWIVTMD
                     ALEPFKTKGPKQSPAPLPYLQQKGKHAFDINLEVYITPEKTDSTLVSKSNFKYMYWSM
                     AQQLAHHTSNGCKVNSGDMMGSGTISGSTEESFGSMLELTWGGKNPIKMKDGSERKFI
                     NDNDTVTMKGYCQNGQIRIGFGEVKSKLLPPFVR"
     gene            4936..6117
                     /locus_tag="P5335_06910"
     CDS             4936..6117
                     /locus_tag="P5335_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74642.1"
                     /translation="MKKITLLIFFYSFISCGVKQSTNHLNSGNYDEAIEIAVNSLRNN
                     KNTKGKQDYVYILEEAFAKAKERDLRNIDSWSKDANPTNLEQIYNSYVQLNRRQELIR
                     PLLPLKLLKEGRDAIFPMDNYSTEIIDSKNALSNYLYTNSKNLLQTANKIEARKVFDD
                     LNYLNSINPGFKDVKKLIDEAQFKGTDFVNVYTKNETNMMIPIRLQDDLLDFSTYGLN
                     DKWTVYHSNRQQGITYDFGIILNFREINISPEQIKEKQVIQEKQIKDGVKNLLDANGN
                     IVRDSLGNPIKVDKFITVKGTAYQFTQFKASQVTAKVDYIDFNNNQLINTYPLASEFI
                     FENIYATFRGDRRAFDEQYISYFGKRAVPFPSNEQMVYDTGEDLKAKLKEIISRNRIR
                     R"
     assembly_gap    6156..6255
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6477..>8719
                     /locus_tag="P5335_06915"
     CDS             6477..>8719
                     /locus_tag="P5335_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRZ74643.1"
                     /translation="MNLKNLTIKSQEALQQAQQIAQGYGQQQLENEHIFKGILEVDEN
                     VAPFILKKMNVNVNLFSQILDSTIQSFPKVSGGEIMLSREAGTMLNEASMIAKKMNDE
                     YVSIEHLILAIFKSKSKIAQILKDQGVNEKGLEAAIAELRKGERVTSASAEETYNSLN
                     KYAKNLNELAKTGKLDPVIGRDEEIRRVLQILSRRTKNNPMLVGEPGVGKTAIAEGLA
                     HRIVDGDVPENLKSKIVFSLDMGALIAGAKYKGEFEERLKSVVKEVIAAEGDIVLFID
                     EIHTLVGAGGGEGAMDAANILKPALARGELRAIGATTLDEYQKYFEKDKALERRFQKI
                     MVDEPDTESAISILRGIKEKYEAHHKVRIKDDAIIAAVELSQRYITNRFLPDKAIDLM
                     DEAASKLRMEINSKPEELDVLDRKIMQLEIEIEAIKRENDETKLKILGLDLANLKEER
                     NEIFAKWKSEKDVVDTIQNVKLEIEDFKQEAERAEREGDYGKVAEIRYGKIKEAQERL
                     DILQTQLIENQSGTSLIKEEVTREDIAEVVAKWTGVPVMKMLQGEREKLLKLEDELHL
                     RVVGQEEAIEAISDAVRRSRAGLQDMKKPIGTFLFLGTTGVGKTELAKALAEYLFDDE
                     NAMTRIDMSEYQERHAVSRLVGAPPGYVGYDEGGQLTEAVRRKPYSVVLLDEIEKAHP
                     DTFNILLQVLDEGRLTDNKGRVADFKNTIIIMTSNMGSHIIQEKFENLKGGIDAATEA
                     AKTEVLG"
     assembly_gap    8720..8819
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8820..9113
                     /locus_tag="P5335_06920"
     CDS             <8820..9113
                     /locus_tag="P5335_06920"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRZ74644.1"
                     /translation="VDIKPKLMYGENGMYRFMSRNFKFPKNEAPVPKIICSFIVETDG
                     TITDIKVLNTIPENFSKEAIRVMTKFDEPWYPAVKDNKQVRCQYILPIIIDRI"
     gene            9213..10376
                     /locus_tag="P5335_06925"
     CDS             9213..10376
                     /locus_tag="P5335_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRZ74645.1"
                     /translation="MFFILFFNLSFGQKVVFESSADVAFVKAKKLNKLVFIEYYNTDC
                     TICMSIQPLFENKEMADFYNNNFVNYKINTKGGLKGKDSLFMAERKLKFTGVPFFLFF
                     DSNKNFVHYSGAKNDLEYLINIGKKALNPEERTGSLQTKYEAGDRSIKTLYAYSDLVQ
                     LYQNDVLSNEISSQLFDVFPKQNLATIQSYLILKNSVKSIDNGFFIYWYENRENLKDF
                     EKGSQKGTEIKVLERILLESIQKQKSTWNLETIRKVKKMTVGLGLSKDGNDFLWEEEA
                     KILVETKQNEEAISLMKILLNESKDNIFSSLYITDFYIKIVESKPTISIVKDELDVLS
                     KLEMEPEQKGDLLYQQLLCFNKMGDTKKSIEFKKTVLEYYQQNGLDLSILDNL"
     gene            complement(10431..10724)
                     /locus_tag="P5335_06930"
     CDS             complement(10431..10724)
                     /locus_tag="P5335_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74646.1"
                     /translation="MTETIPTFFESIEKELVHSLNFPKLDVLESKELKRHRIAGLTRA
                     LALGNIEHIKTKIYFEDSTSKKMVETTIWGITDDKVILKKGAIIPINRIYYSQ"
     gene            complement(10736..11029)
                     /locus_tag="P5335_06935"
     CDS             complement(10736..11029)
                     /locus_tag="P5335_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74647.1"
                     /translation="MATLYESIENEKISSLVFPKSDVLSKNEAILQRISELKLALSFG
                     NLDYFKIKIYFEDNLSKKVVEAKVCGVTNKRVILTQGIGIPINRIYRTFKFYT"
BASE COUNT         3873 a         1740 c         1820 g         3675 t
ORIGIN      
        1 tttcaggatt tggttcaaaa ttatattttc ttttgaaatc tattttaaaa cgattgattt
       61 cctcctgatt atttctttta gaataggaag gatacaacat ttttaatgct ttaaatcgca
      121 tatcggatag accttcccca ctgatatatt ctttaggttg taataatgcc aattgaattt
      181 gatagtcctt cgattctttc aatagaatag tagttgtatt taatatcaaa ccggttttgt
      241 cagtatttaa aacaacataa ttttttctgt ttaaaattag ttcattagta agtgattcag
      301 attctaaaac tcctttactg cttaatttta caaagcgtgc ttttggaaaa ttttgaagaa
      361 taaaatcttc attcagagca tttatggcat cgcttacaac aattaaattt ccgttttttt
      421 cggcaatata attcagcatc atttgagcta aatcattttt agagggaata ctaacaaacg
      481 ttgacggata atttttactg ccctcttctt gtctatttac tatgaatgga atattatttt
      541 ttgaagcaaa atcacctatt ttttcggtgc tgccttttga agaaaagcat aagatagcat
      601 tcgaattttc aactttattt tttttaagtg aaactaactc tgccttcatt tccttattaa
      661 attcaagttc aatagttttt acatcaacct taacattcat tctttttaat gaatctacag
      721 caaatgtagc tccggcataa aaatcaaatt cagttgattg atttgtgctt aacgtttgat
      781 tttttataaa atcttcatat ttttctgttg aaaaaggaag caacataacc acttcttttt
      841 tctccattgt atttaatgtt ttgagtaagc ctatttgttc ttttttgttt tcaacttttg
      901 actcaacttt gttttgggtg atttcaacat ctggaacaac tgtttttgac tccggaactt
      961 tataagcagc agccaattca gtcgtaattt tgtcggatgg aatttttatt atcattccaa
     1021 tctttacacc ttctgctaaa ttaggattaa cttctattaa tttatcttga ctcatcccag
     1081 tcatttttga cagaccaaat aaagtttgct ttggctgaac ttcataatct acccaaccag
     1141 aatctgtact ttgaggaact tcgactttta caggattatc ttttgacttt acattttctt
     1201 gctccacttt tggaacttca accaaaaccg gttcactttt aggaacttct tgttttgata
     1261 ctgcatttga agaaactccc cctgaaacaa caattcgttg accggtttgc aacattttta
     1321 ttatctgagg attttttgct tctaattctt caattgaaat tccgtagcgt tttgacaatc
     1381 cgtattttgt ttctccagct aatacaagat gataattaga attctcagag atagaagaat
     1441 tgtttttctt aaccggaatt ctcaaagttt gtccactctt taaaacacca ttcaaaaaat
     1501 ctttatttgc ttcggttaac tcatctacgg ttaaaccgtt tcgtttcgaa ataccgtaaa
     1561 gggtttcttt tggaagaaca acgtaaataa taaaatctgt aaattgattt acgttttttg
     1621 attccttaga cacataaact tcttttccac ctctgatttc aagcttatga ccagattgaa
     1681 ggccattaac gatatgagga ttttgatttt ctaattgact agttgaaatc ccaaatcgtt
     1741 ttgccaaacc aaactttgtt tccccgggat ttactgtata aattattaca tcattatttg
     1801 aattagatgc attattttca ctcttaggtt ctgtcttttg aagtttagta accaaagaaa
     1861 cgggaatgtt tattacttca ttttccttaa tcccgtttgc caattccgga ttaagtttat
     1921 acaaatcatt tggtgtcact ttatactttt gagcaataga aatcactgtt tctcctttta
     1981 aaacggtgtg ttgtgtaatg ccttcctgag caaataatag gctcaaacca gactgaaaca
     2041 gaagtataat taaaaaataa aattttttca ttcgattaaa ttacaatagt aaataaaaac
     2101 ttacttttct aatttataaa tttaaaaaaa aaccttcaaa agtttttatc ttctgaaggt
     2161 tgttattaag attgttatta acaattttta aaacacgaaa atggctcttt cacccataaa
     2221 atcattcact tcattagcaa cttcctcaat gatttcttcg tttgtaaaat tcataattac
     2281 tcggtcaatt agagcaacaa cggtttccat atccgcctca actaaacctc ttgtagtaat
     2341 agcaggagtt ccaacacgaa ttcccgatgt cacaaaaggt gacttatcat caaatggaac
     2401 catgtttttg ttcaccgtaa tttctgcttt tactaatgca ttttcagctt cttttcctgt
     2461 gatattttta tttcgtaaat caatcaacat catgtgatta tctgttccgc ctgaaattaa
     2521 atcatatcct cttttgacaa atgcttcagc cattgctttt gcattttttt gcacttgaag
     2581 tgaatatcta aaaaactcat ctgataacgc ttcaccaaaa gcaactgctt tggctgcaat
     2641 aatatgttca agtggaccac cttgatttcc aggaaaaaca gcggcatcta ataaagagga
     2701 catcagtctg atttctcctt taggtgtttt tattccgaat ggattttcaa aatctttacc
     2761 catcaaaatc atccctccac gaggaccgcg aagtgtttta tgagtcgttg ttgtgatgat
     2821 atgacaatgt ggaaccggat catttaataa accttttgct attaatccgg cagggtgtga
     2881 aatatctgct aagagtattg ctccaacact gtctgcaatt tctctgaatc ttttaaaatc
     2941 catatctctg gaataagcag atgctcccgc aataatcatt ttaggatttt cttttgttgc
     3001 tatttcctga attttatcat aattcaaacg tccggtttcc ttttcaactc cgtaaaaaac
     3061 aggattatat aatcttcctg aaaaattcac cggtgaacca tgcgttaaat gtccgccatg
     3121 tgacaaatcg aaacctaaaa ttttgtctcc cggttttaaa caagcaaaaa acactgctgt
     3181 attcgcttgt gaacctgaat gaggttggac attagcataa tcggcaccaa aaagggcctt
     3241 tgctctgtcg atggcaattt gttccacttg atccactact tcacaacctc cataataacg
     3301 cttacccgga taaccttcgg catatttatt tgttaaaaca gatccggcag cttccatcac
     3361 ttggtcactc acaaagtttt cagaggcaat cagttctaat ccgtgaattt gtctatcttg
     3421 ctcgtctaaa ataaggtcaa aaatttgttc gtcgcgttgc atttgataat atttcgttaa
     3481 attgagctca aaattacaaa aatcgtttgt taaattgata gtaaataata tatttggttg
     3541 ataatttttc aacattaaat caacaattaa aaatgcctat aacagctaat aacccaaaca
     3601 gaaaatcttg gatagaagtt ccaatcgaca gtgattttcc tattcagaat atcccttttg
     3661 gagtcttttt aactaaggat gatattgtaa ctattggaac aagaattggt gattgtgcta
     3721 ttgatttagg tgctatgcag caattaaatt attttgaagg tatcgattta acggatgata
     3781 tgttcatgca ggatacttta aatgatttca tttctgacgg aaaaaaaacc tggcgtttag
     3841 taagaaacag aattggagat ttatttgata gcaataaccc aaaactaaga gataacaaag
     3901 agcatcggga tatcatcatt tttaaaatgg atgatgtaga aatgcaactt ccagtattaa
     3961 ttggtgatta tacagacttt tattccagta aagaacatgc gacaaacgtt ggtaaaatgt
     4021 ttcgtgatcc cgagaatgca ttgttgccaa attggcttca tattccagta ggctaccacg
     4081 gaagaagttc tacaataatt ccttccggaa tacctgttca tcgtccaatg ggtcaaacgc
     4141 ttccaaatgg agatacccaa ccggtttttg gtccttcaag attagttgat tttgaattag
     4201 aaatggcttt tataactacc gatgctaata taatgggaga aaacattcct gttagtgaag
     4261 cagaagatta tatttttgga atggttttgt tgaatgattg gagtgccaga gacattcaaa
     4321 aatgggaata tgttccgctt ggtccttttt tagcgaaaaa ttttgcttcc tctatttcac
     4381 cttggatcgt tacaatggat gccttagaac cttttaaaac aaaaggtcct aaacaatctc
     4441 cggctcctct tccttattta caacaaaaag gaaaacatgc ctttgatatt aatttagaag
     4501 tttatatcac tcccgaaaaa acagactcca ctttagtgag caaatcgaat tttaaatata
     4561 tgtattggtc aatggctcaa caattagctc atcatacttc taatggttgt aaagtaaatt
     4621 ccggagacat gatgggaagc ggaacaattt caggttccac tgaagaaagc tttggttcta
     4681 tgttggaatt aacctggggt gggaaaaacc caataaaaat gaaagatgga agtgaacgaa
     4741 aattcataaa cgacaacgat actgtaacaa tgaaaggata ttgtcaaaat gggcaaatta
     4801 gaattggttt tggagaagtt aaaagcaagc tactcccccc cttcgtaaga taataatata
     4861 aatccgggta ataccggatt tttttatatc tgattgtact tgtttagtat atttgtgtta
     4921 taaaaatctg atttcatgaa aaaaattacg ttgcttatat ttttctacag tttcatttct
     4981 tgtggtgtta aacagtctac caatcattta aattccggta actatgacga agcaattgaa
     5041 attgctgtaa atagtttacg aaataataaa aatacaaaag gaaaacaaga ttacgtttat
     5101 atacttgaag aagcttttgc caaagcaaaa gaaagagatt taagaaacat cgattcatgg
     5161 tcaaaagacg ccaatccaac aaatttagaa caaatatata attcgtacgt tcaactcaat
     5221 cgtcgtcaag aattaattag gcctttgcta cccttaaaat tactgaaaga aggtagagac
     5281 gctatttttc ctatggacaa ttattctaca gaaattattg atagcaaaaa tgccttgtct
     5341 aattatttat ataccaattc taaaaactta ttacaaacag caaataaaat tgaagcaaga
     5401 aaagttttcg atgatttaaa ctatttaaac agcatcaatc cgggatttaa agatgtcaaa
     5461 aagctaattg atgaagctca attcaaagga actgattttg taaatgttta tacaaaaaac
     5521 gaaaccaaca tgatgattcc tattcgtttg caagatgatt tattggattt tagcacctat
     5581 ggtttaaacg ataaatggac ggtttatcac agcaacagac aacaaggaat tacctatgat
     5641 ttcggcatca ttttgaattt cagagaaata aacatttcac ctgaacaaat caaggaaaaa
     5701 caagtaattc aagaaaaaca gataaaagat ggtgtgaaaa atcttttaga tgccaacgga
     5761 aacatagttc gtgacagttt aggaaatcct atcaaagtag ataaattcat aactgtaaaa
     5821 ggaacagcgt atcagtttac acaattcaaa gccagtcaag ttactgcaaa agtggattat
     5881 attgatttca ataataatca gttaatcaac acttacccac ttgccagtga gtttattttt
     5941 gaaaatattt atgctacttt tcgaggtgat cgaagagcat ttgatgaaca atatatttcg
     6001 tatttcggta aaagagcagt tccatttcca tctaatgaac aaatggttta tgataccggt
     6061 gaagatttga aagcaaaact aaaagagatt attagtagaa atcgaatcag aagatagttc
     6121 atttgatatg ctgacaaatg aaaatttcta aaacannnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnctact ttcgatactt tatcttcttc tgaacttccg aataaattgg
     6301 ttaaaaaact catatttgac acattttaag acattttgtc ttgattttga ataagcttta
     6361 ctcaaaaaaa gacattttgt cttgatttta gaattggaat agtagttgac aattatttac
     6421 caaagatatt gaattaaaag acatttactt gtctgaaaat tataaaaaca caacaaatga
     6481 atttaaaaaa tttaaccatc aaatcgcaag aagcattgca acaagctcag caaattgcac
     6541 aaggttatgg tcaacaacaa ctagaaaacg aacatatttt taaaggaatt ttagaagttg
     6601 atgaaaatgt tgctccattt attttaaaaa aaatgaatgt gaatgtaaac ttattttcac
     6661 aaattttaga tagtacaatt caaagttttc caaaagtttc cggtggagaa ataatgcttt
     6721 cccgcgaagc cggaaccatg ttaaacgaag cttctatgat tgccaaaaaa atgaacgatg
     6781 aatatgtttc aatcgaacat ttaatcttag ccatttttaa atcgaaaagt aaaattgctc
     6841 agattttaaa agaccaagga gtgaacgaaa aaggcttaga agccgctatt gctgaattga
     6901 gaaaaggcga acgtgtgaca tctgcttctg ctgaagaaac ctataattcc ttgaataaat
     6961 acgcaaaaaa tctcaacgaa ttagccaaaa ccggtaagct tgaccctgta attggtcgag
     7021 acgaagaaat ccgaagagtg ttgcaaattt tatcgcgacg aaccaaaaac aatccaatgc
     7081 ttgtgggtga acccggtgtt ggaaaaactg ccattgctga aggtttagca caccgaattg
     7141 tagatggtga tgtacccgag aatttaaaga gcaaaatcgt tttttcttta gacatgggag
     7201 cattaattgc cggtgcaaaa tacaaaggtg aatttgaaga acgtttgaaa tcagttgtca
     7261 aagaagttat tgcagccgaa ggagacattg tattgttcat tgatgaaatt cacacactgg
     7321 ttggagccgg tggcggtgaa ggggcaatgg atgcggctaa tattttaaaa ccggcattgg
     7381 ctcgtggtga attacgtgca attggagcta ccactttaga tgaataccaa aaatattttg
     7441 aaaaagacaa agctcttgaa agacgttttc aaaaaatcat ggtcgatgaa ccggatactg
     7501 aaagtgcgat ttccattttg cgtggaatca aagaaaaata tgaagctcat cataaggtta
     7561 gaattaaaga cgatgccatt attgcagcag tcgaattatc tcaacgctat attaccaacc
     7621 gattcttgcc ggataaagcg attgatttaa tggatgaagc cgcttctaaa ctgcgaatgg
     7681 aaatcaattc aaaacctgaa gaattggatg ttttagatag aaaaataatg caattagaaa
     7741 ttgaaattga agccatcaaa cgtgaaaatg atgaaacaaa attaaaaatt ttaggattgg
     7801 atttagccaa tttaaaagaa gaacgcaacg aaatttttgc caaatggaaa tcagaaaaag
     7861 atgtagtcga tactattcaa aatgtgaaac tggaaattga agatttcaaa caagaggctg
     7921 aacgtgcgga aagagaagga gattatggaa aagtggctga aatccgctac ggaaaaatta
     7981 aagaagcaca agaacgtttg gacattcttc aaacacaatt aattgaaaat cagtccggaa
     8041 cttccctaat aaaagaagaa gtaactcgag aagatattgc cgaagtagtc gctaaatgga
     8101 caggagttcc tgtaatgaaa atgctacaag gtgaacgcga aaaattattg aagttagagg
     8161 atgaattaca tctaagagtt gttggacagg aagaagctat tgaagccatt agcgatgccg
     8221 ttcgtagaag tcgagccgga ttacaagata tgaaaaaacc aattggaact tttcttttct
     8281 taggaacaac aggtgttgga aaaactgaat tagccaaagc gttagccgaa tatttatttg
     8341 acgatgaaaa tgcaatgacc cgaatcgaca tgagtgaata ccaagaacgt catgctgtga
     8401 gccgattagt tggtgctcct ccgggttatg taggttatga tgaaggcgga caactgaccg
     8461 aagctgtgcg acgcaaaccg tattcagtag ttttattaga tgaaattgaa aaagctcatc
     8521 ccgacacatt taatatattg ttacaagtat tggatgaagg gagattaacc gataataaag
     8581 gtcgtgtagc tgatttcaaa aacacgatta ttattatgac atctaacatg ggaagtcata
     8641 ttattcaaga gaaatttgaa aatttgaaag gcggaattga tgctgccacc gaagctgcaa
     8701 aaacagaagt tttaggcttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     8821 tcgacatcaa acctaaactt atgtatggtg aaaacggtat gtatcgtttt atgtcaagaa
     8881 attttaaatt tccaaaaaat gaagctcctg ttcctaaaat tatttgcagt tttatagttg
     8941 aaactgacgg aacaatcact gacataaaag tacttaatac tattcctgaa aatttcagta
     9001 aagaagccat tcgggtaatg actaagtttg atgaaccatg gtatccggct gtaaaagaca
     9061 acaaacaagt gcgatgtcaa tatattttac ctataattat tgatcgaatt tagcatgtac
     9121 tggttaaagt ttgacaaaaa gttttaacaa attgattgca attttataaa tttgtgtaat
     9181 tgtagtaaat tctttttgtt ttgaaatatt tcttgttttt cattttattt tttaatctat
     9241 cattcggtca aaaagtagtt tttgaatcga gtgctgatgt tgcatttgta aaagctaaaa
     9301 agttaaataa gttagttttc atagaatatt acaacacgga ttgcactatc tgtatgagca
     9361 ttcaaccttt atttgaaaat aaggaaatgg cagattttta taataacaac tttgttaatt
     9421 ataaaataaa caccaaaggt ggattaaaag gaaaagatag ccttttcatg gcagaaagaa
     9481 aattaaaatt cacgggagtt cctttctttt tattttttga ttctaataaa aattttgtgc
     9541 actattccgg ggcaaaaaat gatttagaat atcttataaa tattggaaaa aaagctttaa
     9601 atcctgaaga acgaacagga agtcttcaaa caaaatacga agcaggcgac agaagtatca
     9661 aaacacttta tgcatattct gatttggttc aactgtatca aaatgatgta ttaagtaatg
     9721 aaatttccag tcagcttttt gatgtttttc ccaaacaaaa tttagcaact attcaaagtt
     9781 atttgatttt aaaaaattcc gtcaaaagta ttgataacgg attttttatt tattggtatg
     9841 aaaacagaga aaatcttaaa gattttgaaa aaggatcaca aaagggcacg gaaattaaag
     9901 tattagaacg tattttatta gaatctattc aaaaacaaaa atcaacttgg aatttggaaa
     9961 ccattcggaa agtaaaaaaa atgaccgttg gattaggatt atcaaaagat ggaaatgatt
    10021 ttttatggga agaagaagca aaaatattgg ttgaaactaa acaaaatgag gaagcaattt
    10081 cacttatgaa aattctttta aatgaaagta aagacaatat attttcttcc ttatatatta
    10141 ccgattttta catcaaaata gttgaaagta agccaaccat atcaattgtt aaagatgaac
    10201 tagacgtttt atcaaaattg gaaatggaac ctgagcaaaa aggtgatttg ctttatcaac
    10261 aacttttatg tttcaataaa atgggtgata ccaaaaaatc aattgaattt aaaaaaacgg
    10321 tacttgaata ttatcaacaa aatggtttag atttatctat tttggataat ttataaaaaa
    10381 aacaccaacc ggcacagttg gtgttttcaa cttaacaaac taactagatt ctattgcgaa
    10441 taatagattc tgtttattgg aatgattgct ccttttttta ggataacttt atcatcagta
    10501 ataccccaaa tagtagtttc taccattttt ttagaagtac tgtcttcaaa atagattttt
    10561 gttttaatat gttcaatatt tcctagagct aaagctcttg ttaatccggc aatacggtgt
    10621 ctttttagct ctttgctttc taaaacatct agttttggaa aattcagcga atgaaccaat
    10681 tctttctcta ttgattcaaa gaaagtggga atagtttctg tcatatgata gcaatttagg
    10741 tataaaactt aaatgttctg taaattctat taattggaat tccaatacct tgtgttaaaa
    10801 tcacacgttt gtttgtgaca ccacatactt ttgcttctac aactttttta gaaagattat
    10861 cttcaaagta tatcttgatt ttaaaataat caagatttcc aaatgacaaa gctaatttca
    10921 actcggaaat acgttgaaga attgcttcat ttttacttaa tacatcagac ttaggaaaca
    10981 cgagagaaga aattttctcg ttttcaatgg attcataaag agtcgccata gtgctaaatt
    11041 taggttaata atagatttaa ggggactcaa atttaacata atattttaaa taacaacatt
    11101 ttttttgact tttttttgat tattttcaaa ataaagttga aatgtatatt gaataattga
    11161 tttagttatt gtaaaaaaca tcaattagta ttaaaaattt caaattatta ttttgcagaa
    11221 aatatcgttc gtttattact aattcatata aaataaaaat cccttgagta tttctcaaag
    11281 gatttgtttc gtcgggatga ctggattc
//
LOCUS       DAOTCW010000068        11238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3728_length_11238_cov_31.007690, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000068 DAOTCW010000000
VERSION     DAOTCW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3728_length_11238_cov_31.007690"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..649)
                     /locus_tag="P5335_06940"
     CDS             complement(158..649)
                     /locus_tag="P5335_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRZ74648.1"
                     /translation="MQYDAKAPFIVETHWQIEEVLDGDSIIICNRFTQLKKEIRLYGL
                     DAPEVKINRKMMEDEEKSSLPAELLLQLGLQSLQFVLSVAPPKTSVTIITELENYYDY
                     WNRQLGYVILPGGECLNDLLLINGYAKAANNYFCSKLAHYQILNRDAQLNEIGIYSQV
                     KRF"
     gene            747..1550
                     /locus_tag="P5335_06945"
     CDS             747..1550
                     /locus_tag="P5335_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74649.1"
                     /translation="MARQKGNLKYVGTLGDVRHFKIKGQEGYFAGMVGGPTGDQVLTA
                     PEFERTRENMNEFGACAKAGKSVRTGLSQVMKQMADSQVTGRLTSIMKKINLEDQTEA
                     RGYRKIEITTQRNYLKGFEFDKNTSFNGIFNAPFTLTHEVTRETGDFVIPAFNPLNLV
                     NAPSGATHFRLIHSLSVVSDFEYNATTNNYDPMDAELNEVNDIQYSGYLDLFAPVPLT
                     TITATLPNGAIPTANTTVLQCIGIEFYQQVGGNYYLFASGNCLKVEDAF"
     gene            1619..2071
                     /locus_tag="P5335_06950"
     CDS             1619..2071
                     /locus_tag="P5335_06950"
                     /inference="COORDINATES: protein motif:HMM:NF040141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5675 family protein"
                     /protein_id="HRZ74650.1"
                     /translation="MESKIIRVAQGKESTLSQLYINGIFQCYLLEDKIREVKIPSQTA
                     IPTGVFTLKLNTHGAKNVQYKRAFAELHEGMIEIVGLPNFSFVYIHVGNTIKDTAGCP
                     LCGFGFQFNDGNYKVTQSVAAYKMIYPKLVALAKQPTNKIIVENNFQF"
     gene            2084..2350
                     /locus_tag="P5335_06955"
     CDS             2084..2350
                     /locus_tag="P5335_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74651.1"
                     /translation="MENINIIATLFGTLITLLLSIVAYFIKQLHTDFKSMEKDLVEVK
                     TMALIIKTEFKSGNDLLNQKVEFLEKRVSKIELTLFKNQDYEKQ"
     gene            2337..2579
                     /locus_tag="P5335_06960"
     CDS             2337..2579
                     /locus_tag="P5335_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74652.1"
                     /translation="MKNNNLNVVERALAPTPKWFKILRTVGIAMASVGGVVLASPVAL
                     PVGLVSAAGYLILGGSIISVVSQTAVKSEETPEPKE"
     gene            complement(3266..4510)
                     /gene="ilvA"
                     /locus_tag="P5335_06965"
     CDS             complement(3266..4510)
                     /gene="ilvA"
                     /locus_tag="P5335_06965"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HRZ74653.1"
                     /translation="MIHLAKIQEAAENLKGVAVHTPLVKNENLSERFAAQVYLKREDL
                     QPVRSYKLRGAYHKISSLSENDIKLGVVCASAGNHAQGVAFACRKLNIKAVIYMPITT
                     PAQKIKQVKLFGKENVEVVLKGDTFDDAYNEALLFSQKNKAVFVHPFDDELVISGQGT
                     VGLEIVEDSKCKIDFLFFPIGGGGLAAGVVSVFKQLSPETNLIGIEPQGAASMKTSLE
                     NGKNTALTNIDKFVDGAAVKRVGDKTFEICQHSLDDIVLVPEGKVCTTILQLYNEEAI
                     VVEPAGALSIAALDSYKDQIKGKNVVCIVSGSNNDITRMEEIKERSLMFEGLKHYFII
                     NFPQRPGALKEFVNDVLGENDDITYFQFTKKNNREEGPAVVGVELKYSSDLDSMEQKM
                     KTKKINYQHLNEQKELFTHLIF"
     gene            complement(4507..5553)
                     /gene="ilvC"
                     /locus_tag="P5335_06970"
     CDS             complement(4507..5553)
                     /gene="ilvC"
                     /locus_tag="P5335_06970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRZ74654.1"
                     /translation="MAQLNFGGVMENVVTREEFSLEKAREILKNETVAVIGYGVQGPG
                     QALNLKDNGVKVIVGQRKGTKSWDKALADGWVENETLFEVETACEKGTLLMNLLSDAG
                     QIQAWETMKKNLTTGKALYFSHGFGVTFHEKTGIVPPKDVDVFLVAPKGSGTSLRTLF
                     LKGQGLNSSYAVFQNATGKAEEKALALGIAIGSGYLFETTFQKEVYSDLTGERGVLMG
                     AIAGVFEAQYNVLRQRGHSPSEAFNETVEELTQSLMPLVAENGMDWMFANCSTTAQRG
                     ALDWKGKFREATTPVFNELYDDVLSGKEAAIVIEANSKPDYREKLNAELKEIQQSELW
                     QTGMQVRKLRPQTK"
     gene            complement(5574..6104)
                     /gene="ilvN"
                     /locus_tag="P5335_06975"
     CDS             complement(5574..6104)
                     /gene="ilvN"
                     /locus_tag="P5335_06975"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRZ74655.1"
                     /translation="METMNKTFTVSIFTENTIGMLNRITIIFTRRHLNIDSITASETE
                     VKNVHRYTIVLRTNREQIDKVVGQIDKLIDVLKAFVHEDNEVVHQEIALYKIKTTELK
                     SNNVEQVVRENSAKVLTVDPDFIVIEKTGHKADTQVLFEKLKPFGILEFARSGRVAVT
                     KPMKELSTYLKELEIN"
     gene            complement(6121..>7122)
                     /locus_tag="P5335_06980"
     CDS             complement(6121..>7122)
                     /locus_tag="P5335_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRZ74656.1"
                     /translation="EKTGIPVASTLLGLGAFPSHHPLFVGMVGMHGNYAPNVKTNECD
                     VLLAVGMRFDDRVTGDVSRYATQAKVVHVDIDTAEINKIIKADAPVVADAKEALTVLA
                     DLVEKQTHQNWLDEFHQAKQKELQVLSENREKEFSDELRMDLVIDLLSQKTKGNAIVV
                     TDVGQHQMMAAQFYQYNQTKSNVTSGGLGTMGFALPAAIGAKMANPEKQVVAIIGDGG
                     FQMTLQELGTMMQNNIGVKIIILNNGYLGMVRQWQQMFFEKRYSFTDIQSPDFVALAA
                     SYNIKGQKVERQEDLNNALDQLLNTENAYLLEVKVAREDNVFPMVPTGASVAEIRLQ"
     assembly_gap    7123..7222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7223..7371)
                     /locus_tag="P5335_06985"
     CDS             complement(<7223..7371)
                     /locus_tag="P5335_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRZ74657.1"
                     /translation="MKTTELKITGAEAVIQSLKAEGVKTIFGYPGGAIMPIYDALFHH
                     LEEVN"
     gene            complement(7379..9055)
                     /gene="ilvD"
                     /locus_tag="P5335_06990"
     CDS             complement(7379..9055)
                     /gene="ilvD"
                     /locus_tag="P5335_06990"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRZ74658.1"
                     /translation="MKTLNKYSRTITQDETQPAAQAMLYGIGLTEDDLQKAQVGIVSM
                     GYEGNPCNMHLNDLAKEVKAGVQQEDLVGLIFNTIGVSDGISNGTDGMRFSLVSRDVI
                     ADSIETVVSAQWYDAVLAVVGCDKNMPGSIIAMGRLNRPAIMVYGGTIHSGKWKGESL
                     NIVSAFEALGKKFSNTISDEDYKGIIKNACPGAGACGGMYTANTMASAIEALGMSLPY
                     SSSNPALSSNKKMECFDTGKAIKVLLEKDIKPKDIMTRKAFENAMTMVTVLGGSTNAV
                     MHLIAMAHSVDLELTLNDFQKVSNRVPVLADLKPSGKYLMEDLHEAGGVPAVMKYLLK
                     HNLLHRDCLTVTGKTIVENLEAVEDLTEGQKVFLPLEKPVKANGHLQMLYGNLATEGS
                     VAKISGKEGDYFEGTAKVFDDEYAVIDGVRNGEVKPGNVVVIRYCGPRGGPGMPEMLK
                     PTSAIMGAGLGNSVALITDGRFSGGTHGFVVGHITPEAFVGGTIALVNDGDLIAIDTK
                     NNTINLKVSEQELEKRKAAWKQPPLKANKGVLYKYAQCVSSASLGCVTDK"
     gene            complement(9055..9942)
                     /locus_tag="P5335_06995"
     CDS             complement(9055..9942)
                     /locus_tag="P5335_06995"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HRZ74659.1"
                     /translation="MYSNNETVLFLDGKFVKANESTTDLYSQTLHYGYGAFEGIRSYE
                     TKNGTKVFKAEEHYERLKKSCELVKIPFDCTVQELVQATYELLEKNNLKNAYIRPLVY
                     CGQNMSLSKPTRVSVMIAAWDWGAYLGEKLLRLTVSSYCRPHPKSIHIEAKVCGHYVN
                     SILATNEAKDNGFDEALLLDSDGFLAEGPGANLFFEKDGKLFTPQLGNILPGITRATV
                     LELAEQLGLELHQGKFTREDLFQADSAFYCGTAAEVVGILSVDTYQFPKNWNDSLGKK
                     LQDAYSLLVREPLKQLNLN"
     gene            complement(10430..10969)
                     /locus_tag="P5335_07000"
     CDS             complement(10430..10969)
                     /locus_tag="P5335_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74660.1"
                     /translation="MSFFDKTYNNIINNQEYLNRLGKIHPKVPTILEMQSSNSSDAIL
                     MNIFAHPDVKDWKPLSDLLSINSNDDIEFGWNPVHENEKWKKTEIDVKIGNSIFEAKL
                     TETDFKSKHLNIVLRYVDVEKIIDLKALTKDEIVSNYQLIRNIVTAKKYGYKFHLLID
                     VRCNPKRRNNRRTKCQPGI"
BASE COUNT         3169 a         2237 c         1899 g         3833 t
ORIGIN      
        1 aaacaacatc aatgaagttt gttccaattc ctatcaagat ttttatggtt atcaactggg
       61 tacaactact ttatatactc cttacacccg taaggtactg tatttattga ttattattac
      121 tttttgggtt ctcttagtac aacataagta caacaaacta gaaccttttt acttgactat
      181 aaatacctat ttcattgagc tgagcatcac gatttaatat ttgatagtgt gctaatttac
      241 tacaaaaata attatttgct gcttttgcgt atccattgat caaaagcaag tcatttaaac
      301 attcccctcc tggaaggatt acatagccta attgccggtt ccaatagtca tagtaattct
      361 ctagttctgt aatgatagtt acagacgttt taggcggtgc aaccgataaa acgaactgta
      421 aggactgcaa acctaattga agcagtaatt cagccggtaa actgcttttc tcctcgtctt
      481 ccatcatctt tctgttaatc tttacttccg gtgcatctaa accataaagt cggatttctt
      541 ttttcaattg agtgaaacga ttgcaaatga ttatgctatc cccatcaagc acttcttcaa
      601 tttgccaatg ggtttccact ataaacggtg cttttgcgtc gtattgcatt gattttcaat
      661 gttttaagtt gttgataatc aaagataatg attgaaattt gtcttatcaa tttaatctaa
      721 ttattaaccc attaaaactt aaaaaaatgg caagacagaa aggcaatttg aagtatgttg
      781 gaactcttgg agatgttcgt cacttcaaaa ttaaaggaca ggaaggctac tttgcaggca
      841 tggtaggcgg accaactggt gatcaagtat taactgcacc agaatttgag agaactcgtg
      901 agaacatgaa cgaatttggt gcttgtgcca aagccgggaa atccgttcga acaggtttgt
      961 ctcaagtaat gaaacaaatg gcggatagcc aagttaccgg aagattaact tcaatcatga
     1021 agaaaatcaa cctggaagat caaacggaag ccagaggtta ccgtaaaatt gaaatcacaa
     1081 cccaaagaaa ttacctaaaa ggatttgagt ttgacaaaaa cacttccttt aacggtattt
     1141 tcaatgctcc tttcacttta actcatgaag ttacaagaga aacaggtgat tttgtaattc
     1201 ctgcatttaa tccgttgaat ttagtgaatg caccttccgg agccactcat ttccgcttga
     1261 ttcattcatt atctgttgta agtgattttg aatacaacgc taccacaaac aattatgatc
     1321 caatggatgc ggaattgaat gaggtaaacg atattcaata tagcggatat ttagacctgt
     1381 ttgctccggt tccattgact accatcacag caactttgcc aaatggagct attccaaccg
     1441 ctaatacaac tgttttacag tgtatcggaa ttgagttcta ccaacaggta ggtggaaatt
     1501 actacttgtt tgcaagtggt aactgcttga aagttgagga cgctttttag aaaaaccctt
     1561 caaaaatccc tcatcacaag tgagggattt ttcattaaat cttaaatcta tcaaaacaat
     1621 ggaaagcaaa ataataagag tagcccaggg caaagaaagc accttgagtc aattgtatat
     1681 caacggaata tttcaatgtt atctgttaga agataaaatc agagaagtga aaataccttc
     1741 acaaactgct attccaacag gtgtttttac tttgaaacta aacactcatg gagctaaaaa
     1801 tgtgcagtat aagagagcat ttgccgagct tcacgaaggc atgattgaaa tagttggttt
     1861 gccaaatttc agttttgtgt acatccatgt cggaaatact attaaagata ctgccgggtg
     1921 tcctctttgt ggttttggtt tccagtttaa tgatgggaat tacaaagtaa ctcagagtgt
     1981 tgcagcttat aaaatgattt atccaaagtt ggttgcattg gcaaaacagc ccacaaacaa
     2041 aatcattgtt gaaaataatt ttcaatttta aaaagtgtga aaaatggaaa atatcaacat
     2101 catcgcaaca ctttttggaa cactcattac cttgttactg tcgattgtgg cttattttat
     2161 caagcaatta cacaccgatt tcaagagcat ggaaaaagat ttggttgaag taaaaacgat
     2221 ggctttaatc atcaaaaccg agttcaaaag tggtaacgac ttactgaacc aaaaagtaga
     2281 atttttagaa aaacgagtat caaaaattga attaacacta tttaaaaatc aagattatga
     2341 aaaacaataa tttgaatgta gtagaaagag cattggctcc aaccccaaaa tggtttaaaa
     2401 ttcttcgcac tgttggcatc gcaatggcat ctgttggcgg agtagtactg gcttctccgg
     2461 tagctttacc ggttggattg gtttcggcag ccggatattt aatcctaggc ggaagtatta
     2521 tttcagtagt ttcacaaacg gctgtgaaat ctgaagaaac tcctgaacca aaggaataaa
     2581 aacccttaaa aatcaatttt aaagctccta atttcggagc tttttttgtt tttagcagtc
     2641 aatacatgtc aaatgctttg cttttcttcg atttatttaa gaggagcatt tatattgctt
     2701 ttatgtagtc aatatgtagc gtttaaggtg caaaaaaaac ttattcaagt ggaccagaat
     2761 tggactttag tatttattaa ttcgtcacga gtttttaaaa atatttttcc gttagaaatt
     2821 cttttaaatc atcactcaca caagttgata tttgttgcca agttataagt tcaatactat
     2881 ttgccaagga tttatctaaa atcgaatttt tcactttatc aaattcattt cgaagatctg
     2941 ttctactttc ggctacggct ttgagaagtt gcggccggta gttccaaagc taataaaaca
     3001 agatggattt tcaatcacct gagtactctc tgaaagcgca caaaaccagc aattttttaa
     3061 agccgaggtt aggtgctgcc cttcttttct gtcttgttgt tgtctgtcca ttttactcct
     3121 gcttggtgcg ttggcttggt ggctcttttg caaaacttgg ctttggcctt tgcctgtgcg
     3181 gttttgcaaa tgtgccacca aatgcgttgg ctaaatatta atgaactgtt gtttgaattt
     3241 tacaacacat actgtacaag atagtttaaa atatcaaatg cgtaaacaat tctttctgct
     3301 cattcagatg ctgataattt atttttttag ttttcatttt ttgttccata ctatctaaat
     3361 cagaactgta ttttagttct acgccaacaa cagccggacc ttcttcgcgg ttgttctttt
     3421 tagtgaactg gaaataagta atgtcatcat tttctcctaa gacatcattt acaaattctt
     3481 ttaatgctcc tggtcgttgt ggaaagttga tgatgaaata atgcttcaat ccttcaaaca
     3541 tcaaggatcg ctcttttatt tcttccattc ttgtaatgtc attgttgctg ccactaacaa
     3601 tgcacaccac atttttacct ttgatttggt ctttatacga atccaaggct gcaatactca
     3661 atgcaccagc tggttctaca acaatggctt cttcgttata taattgtaaa attgttgtgc
     3721 aaactttccc ttcaggcact aagacgatgt catctaacga atgttggcag atttcaaatg
     3781 ttttgtcgcc cacccgtttt acggcagctc catctacaaa tttatcaatg ttcgtcaatg
     3841 cagtattttt accgttttcc aacgatgttt tcatagaggc tgctccttgt ggttctatac
     3901 cgataaggtt agtttcagga ctcaattgtt taaaaaccga aacgacccct gctgccaaac
     3961 cgccacctcc aattgggaag aataaaaaat caattttaca tttgctgtct tccacaattt
     4021 ccaatcctac agttccttgt ccggaaataa ccaattcatc atcaaacgga tgaacgaaaa
     4081 ctgccttatt cttctggctg aaaagaaggg cttcgttgta ggcatcgtca aaagtatcac
     4141 ctttcaacac aacctctaca ttttcttttc caaagagttt tacctgcttt attttttgtg
     4201 caggcgtggt aataggcata taaataacgg cttttatgtt cagttttcta cacgcaaaag
     4261 ccactccttg agcgtgatta cccgcacttg cacatacaac gcctagtttt atatcatttt
     4321 cggaaagcga acttatttta tgatacgctc cacgcaattt gtaggatcgt acaggttgta
     4381 aatcttcccg tttcaaataa acctgagcag caaatcgctc gctcaggttt tcgtttttca
     4441 caagaggcgt atgcacagcc acacctttca ggttttcggc tgcctcttgt atttttgcta
     4501 aatgtatcat ttggtttgag gtcttaattt tcgaacttgc attccagttt gccacaactc
     4561 actttgttgt atttctttga gttctgcgtt gagtttttct ctgtaatctg gcttgctgtt
     4621 ggcttcgata acgatggctg cttctttacc tgaaagcacg tcatcataca gttcattaaa
     4681 aacaggagtg gtagcttctc taaatttacc tttccaatcc aatgctcctc gttgtgcagt
     4741 ggtactgcaa ttggcaaaca tccaatccat tccgttttcg gctaccaaag gcattaaact
     4801 ttgggtcaat tcttctacag tttcgttgaa ggcttcactt ggcgaatgtc ctcgctgacg
     4861 aagcacatta tattgagcct caaaaacacc tgcgatagct cccatcaaaa cgcctcgttc
     4921 gccagtcaaa tcgctgtata cttctttttg aaatgtggtt tcaaacaaat aaccagaacc
     4981 gatagcgatg cctaatgcca atgctttttc ttcagctttt ccggttgcat tttgaaaaac
     5041 ggcataactg gaatttaaac cttgcccttt taaaaataat gttcttaacg aagttcccga
     5101 acctttggga gccaccaaaa acacatctac atctttaggt ggcacgatac ctgttttttc
     5161 atgaaaagta acgccaaaac catgtgaaaa atacagggct tttcctgttg tcaaattttt
     5221 tttcatcgtt tcccatgctt gtatttgccc tgcatctgat aataaattca tgagtaaagt
     5281 gcctttttcg caagcggttt ccacttcgaa aagagtttca ttttctaccc aaccatcagc
     5341 aagtgctttg tcccaactct ttgtcccttt tcgttgtcct acaattactt ttacgccatt
     5401 atctttcaaa tttaaggctt gtcccggacc ttgcacgccg tatccaatga ctgcaacagt
     5461 ttcgtttttt aatatttctc ttgctttctc taatgaaaac tcttctcgtg ttacgacatt
     5521 ttccattacg ccgccaaaat ttaattgtgc cattttatat tgtattattt tttttaattg
     5581 atttctaatt cttttaaata agtacttaat tctttcattg gtttggtaac tgctactctt
     5641 cctgagcggg caaattccag aataccaaag ggtttcaatt tctcaaataa aacctgtgta
     5701 tctgctttgt gccctgtttt ttcaatgacg ataaaatcgg gatctacggt gagaactttg
     5761 gcactatttt ctctaaccac ttgctctaca ttgttggatt taagttctgt tgttttgatt
     5821 ttatataacg caatttcctg atgtactact tcattatctt cgtgtacaaa ggctttcaga
     5881 acatcaatta atttgtcgat ttgcccaaca actttatcta tttgttctct gtttgttctc
     5941 aaaacgatag tgtaacggtg cacattttta acctctgttt cagaagcggt gatgctgtca
     6001 atattcaaat gtcgtcttgt aaatatgatg gtgatgcggt tgagcattcc gattgtattt
     6061 tccgtaaata tggatacggt aaatgttttg ttcattgttt ccattttatc cgttttataa
     6121 ttattgtaat cttatttcag ctaccgaagc acctgttggc accatcggaa aaacattatc
     6181 ttctctggca actttcactt ccaacaggta cgcattttct gtgtttaaaa gttggtctaa
     6241 tgcgttgttc aagtcctcct gtctttcaac tttttgacct ttgatgttgt aagaggctgc
     6301 caaagccaca aaatcaggac tttgaatgtc ggtaaacgaa tatctttttt caaagaacat
     6361 ttgttgccat tgcctcacca ttcccaaata accattgttt aagatgatga ttttcacgcc
     6421 gatattattc tgcatcatgg ttcccaattc ctgcaaggtc atttgaaaac caccatctcc
     6481 aatgatagct acgacttgtt tttccggatt tgccattttt gctccaatag cggctggaag
     6541 tgcaaaaccc atcgttccca atccgccaga tgtaacatta cttttggttt gattatactg
     6601 atagaactgt gccgccatca tttggtgctg tcccacatcg gttacaacga ttgcattgcc
     6661 cttggttttt tgagaaagta aatcaatcac caaatccatt ctcagttcat ctgaaaattc
     6721 cttttctcta ttttctgaaa gtacctgtaa ttccttttgc ttcgcttgat gaaattcatc
     6781 caaccaattt tgatgagttt gtttttctac caaatctgct aaaacggtta gtgcttcttt
     6841 ggcatcggct acaacaggag catcggcttt tatgatttta ttgatttcgg cagtgtctat
     6901 atcaacatga accacttttg cttgtgttgc ataccgggaa acatcacctg taacacggtc
     6961 gtcaaaacgc attcccacgg caagcaacac atcgcactca ttggttttta cattgggtgc
     7021 ataattgcca tgcatgccta ccattccaac aaataaaggg tgatgacttg gaaaagctcc
     7081 taatcccaaa agcgtagaag ccaccggaat acccgttttt tcnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nntgatttac ttcttcaaga tgatgaaaca aggcatcata
     7261 aattggcatg atagcaccgc ctggataacc aaatatcgtt tttacacctt cggctttcag
     7321 gctttgtatc accgcttctg cacctgttat ttttaattcc gttgttttca ttttttaatt
     7381 atttatcggt tacacacccc aatgaagcac tcgaaacgca ttgtgcatat ttgtacaaaa
     7441 cccctttatt tgctttcaat ggaggttgtt tccatgctgc ttttcgtttt tccaattcct
     7501 gttcagatac ttttaaattg atcgtattat tttttgtgtc aatcgcaatc aaatctccat
     7561 catttaccaa ggcaattgtt ccacctacaa aagcttccgg cgtgatgtgt cctaccacaa
     7621 aaccgtgtgt tccacccgaa aatctaccat cggtgattaa agccactgaa tttcccaatc
     7681 ctgcacccat aattgctgaa gttggtttga gcatttcagg cattcccggt ccgcctcttg
     7741 gtccgcaata acggattact accacattcc cgggttttac ttctccgttt cgcacaccat
     7801 caatcactgc atattcatcg tcaaatactt tggctgttcc ttcaaaataa tcgccctctt
     7861 tgccacttat tttagctaca cttccctcgg tagctaaatt tccgtagagc atttgcagat
     7921 gtccatttgc tttcactggt ttttccaatg gaagaaatac tttctgacct tcggttaaat
     7981 cttccacagc ttccaaattt tcaacaattg tttttcctgt aacagttaaa caatctctat
     8041 gtaataaatt gtgtttcaat aaatatttca ttactgctgg aacaccacct gcttcgtgca
     8101 aatcttccat gaggtatttt ccacttggtt ttaaatctgc taacactgga acacggttac
     8161 ttaccttttg gaaatcattc aaagtcaatt ccaaatccac cgaatgtgcc attgctatca
     8221 ggtgcattac ggcattggta gagccgccca aaaccgttac catggtcatg gcattttcaa
     8281 aggcttttcg ggtcatgatg tctttgggct taatgtcttt ttccaacaac actttgatgg
     8341 cttttcctgt atcaaaacat tccatttttt tattgctgct caacgcagga ttggatgaac
     8401 tgtaaggtaa actcattccc aaagcttcaa tagccgaagc catcgtattt gctgtgtaca
     8461 taccgccaca agcacccgcg ccaggacaag cgtttttgat tatacctttg taatcttcat
     8521 cggaaatggt gttactgaat tttttaccta aagcttcaaa agctgaaacg atattgagcg
     8581 attctccttt ccattttcca gaatggatcg ttccgccata aaccatgatt gcaggacgat
     8641 ttaatcttcc catggcgatg atagaaccag gcatattttt atcgcaacct actaccgcca
     8701 atacagcatc gtaccattgg gcactcacca ccgtttctat ggaatcggcg atcacatctc
     8761 tggaaaccaa agaaaaacgc ataccgtcag ttccgtttga tataccgtca ctcacaccaa
     8821 tggtattaaa tatcaatcct actaaatcct cttgttgcac accagctttt acttctttag
     8881 ccaaatcgtt gaggtgcatg ttgcagggat ttccttcgta acccatactc acaattccca
     8941 cttgtgcttt ttgcaaatcg tcttcggtta aacctattcc ataaagcatg gcttgtgctg
     9001 caggttgggt ttcatcttgt gtgatggttc tactgtattt gttgagtgtt ttcattagtt
     9061 taaattgagc tgttttaaag gttctcgtac caaaagtgaa taagcatctt gaagtttttt
     9121 acctaaggaa tcattccagt tttttggaaa ttgataagtg tccaccgata atatacccac
     9181 tacctcggca gccgttccgc agtaaaaggc actatcagct tgaaaaagat cttctctggt
     9241 aaattttcct tgatgcagtt ccagacctaa ttgttcagct aactccaaaa cagtggctct
     9301 ggtgatgccc ggaaggatgt ttcctaattg aggcgtgaat agttttccgt ctttttcaaa
     9361 gaacaaattt gcacccggac cttcggctaa aaatccatcg ctatccaaaa gcaaggcttc
     9421 gtcgaatcca ttgtcttttg cttcgttggt tgccaaaatc gaattgacat aatgaccgca
     9481 gactttcgct tcaatgtgga tggattttgg atgtggacgg caataggagg aaaccgttaa
     9541 tcgtagtaat ttttctccca aatacgctcc ccagtcccaa gcggcaatca tcaccgaaac
     9601 cctggtaggt tttgacaaac tcatattttg tccacaatac accaatggtc ggatgtaagc
     9661 attttttaaa ttgttttttt ccagtaactc gtaagtagct tggactaact cttgaacagt
     9721 gcaatcgaaa gggattttta ccaattcgca ggattttttc aagcgttcat aatgttcttc
     9781 ggctttaaat actttagttc catttttggt ttcataagaa cgaatacctt caaatgcacc
     9841 gtagccataa tgaagcgttt gactgtacaa atcggttgta gattcgttag cctttacaaa
     9901 ttttccgtcc aaaaaaagga ctgtttcgtt gtttgaatac atatttattt tttttattga
     9961 gttgataatt gatttttggg aataaaaaaa gcctttcccg aaatgagaaa ggcttacaat
    10021 ttggcataaa gcagcatctc tcacggtggc gagctaataa taatgctgat aataatagtg
    10081 ttgttatatg atttcatttt gttcttttta gtttaatgtc aataaaaaaa gcctcctgat
    10141 ttctcgggag gctttcaaat atatctttta gattatatca aaattattcc tccccggtat
    10201 gtgaaatcac acccgcaata ataatagaag aaataataat tgaattgtac attttttttc
    10261 tgttttaacg gtacaaatgt aagtatactt ttttaatgtg caaatttttt ataccttttt
    10321 tttgaaagtc gttaaaattc agtagacaga tgtaggtttg ggtgaggttg ggaaaataaa
    10381 atgtgctgca atttgtgtag gcttgtgcgt tggcttgcag ctctttttat tatatgccgg
    10441 gttggcattt tgtacgtcgg ttgtttcgtc ttttagggtt gcacctaacg tctattaaca
    10501 gatgaaattt ataaccgtat ttctttgcag tcacaatatt tcttatcaat tgataattag
    10561 aaacaatttc atcttttgtt aaagctttta agtctattat tttttctaca tcaacatatc
    10621 gcaatactat attcaaatgt ttacttttaa agtcagtctc tgttaattta gcttcaaaaa
    10681 ttgaattacc aatcttgaca tcaatttcag tttttttcca cttttcgttt tcgtgaacag
    10741 ggttccatcc gaattcgata tcgtcattag aattaattga aagtaaatcg ctaagtggtt
    10801 tccagtcttt aacgtccgga tgtgcaaaaa tgttcattaa tattgcatct gaactgtttg
    10861 aagattgcat ttctaaaatg gttggaacct ttgggtgaat tttacctaaa cgatttaaat
    10921 attcttggtt gttaattata ttattatatg tcttgtcgaa aaaactcatt tcaattttgt
    10981 caaacaaaaa tgcactcggt ctttctattc cagataaaga tctgttctta caaaagttta
    11041 aagcatttgc tattaatctt tttttaagtt gcattgaatt gtccataagg ttccttaata
    11101 tattattaca ttatatatca ctagtataca tttatcaaat atatacaata aaaaatagat
    11161 tttatttatt gcagtagcgg tggagaatta ctcgacgcat atcgtttatg aggttgatgt
    11221 tttcgtgaaa ttgttttt
//
LOCUS       DAOTCW010000069        11219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3738_length_11219_cov_46.926012, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000069 DAOTCW010000000
VERSION     DAOTCW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11219
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3738_length_11219_cov_46.926012"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..3086)
                     /locus_tag="P5335_07005"
     CDS             complement(36..3086)
                     /locus_tag="P5335_07005"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRZ74661.1"
                     /translation="MKTKLLSTIFFLCFVNFFSYSQTQLSACDTDYNGTETFDLTSII
                     PTLLNGQNPNDFDISFHTSLINAEENSNAITNPTSYNSMIGFVTLFARLQNINTSDYE
                     IVTFTIVVNSVPNIGTEVAVACDDDNDGIAFFDLNQAVQTIYAANNASPNTMSVTFYL
                     TYADMLNQVNPIGYNYTNTSPNQIIFVRIEDFITGCFWGGEFFLLVQNCSTSCETPNN
                     LNAINSTENGATLGWFNPSATNTNEIYIVPFGSPEPNPNSQGFIASVNPFVITALTCD
                     TSYTFYVRAICNDQITSDWSAGFNFSTTSCGTTTIPFVTVNTTSFTPEQILNNVILNS
                     DCASISNVITQGPCGVGYFNANDSSFPFEQGLIIRSGNANLTQGPYTDANYTSNCSQI
                     GDPQLTAIMQAAGQTGTINDVSFVKFDIVPTTNILSFNFIFASNEYGAYQCQFSDVFG
                     FILTDLTTNQTQNIALVPGTNVPISTTTIRDNIYNTSCASVNPQFFSTYNVNNPTSSL
                     NMRGYTVPMTAIASVIPNHEYSLKLAVGDYQDSAFDSAVFIEGGSLALGNQCQDNIQL
                     IAFLDSNNNGIKDSGEQNFVNGNFNYQVNNTGDVVSNISSNGVFYIFPENTTNTYDLS
                     YAVYSEYAGYYAQPISYDDIVFSETESNVYYFPIVNTTSYNDVAVSIVATNQPTAGFT
                     YQNKLIYTNNGIAPASGTLTFDKAPVLTITGISQTGTTPSANGFTFDYTDLLPLETRT
                     INVSMSVPAIPTVAIGDLVTNSVSISTTTLDANSTNNSASLTRPIVASYDPNDKNEAH
                     GKEIVLAQFSQDDYLYYTIRFQNTGTTNATFVRVEDILDAQLNPESLRMIASSHNYVL
                     ERINNQLVWKFENIHLVPKVVNENASIGFIHFKIKTNPGISIGDIIPNTAEIYFDFNP
                     AIITNTFETEFVASLSTADFTYSDLVLFPNPAKDLIQIQLKNGFDSIKGIIIYDVLGK
                     EVYSKQNMSMEQTAIDISRYTKGVYIVEISTVNNQKVLQKFVKQ"
     gene            complement(3167..4384)
                     /locus_tag="P5335_07010"
     CDS             complement(3167..4384)
                     /locus_tag="P5335_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRZ74662.1"
                     /translation="MELMYDVLIVGGGAAGFFTAINVAEKNPEFSIAILERGKEVLTK
                     VSVSGGGRCNVTHACFVPNDLVKFYPRGEKELRGPFHQFCSGDTIEWFSNHGVELKIE
                     DDGRMFPVSDSSQTIIDCFLTVAKKHKIEILTGQSVQSLYQAEDTWKVETNHQTFRCK
                     NLVMTTGSNPKIWELLENLGHTIVPPVPSLFTFNIKDPRIKDLMGVSAFATVKVQNSK
                     LSASGPLLITHWGMSGPGILRLSAWGARELFAKNYQFMLEVNWLNDATLEETESHLKE
                     LKLEHAKKVVSKKSPFEFPNRLWESLVLASEISLETKWADLTKKQLQHLALQLTNGKF
                     QVNGKSTFKEEFVTAGGIDLKEINFKTMESKVLPKVYFAGEIVNIDAITGGFNFQNAW
                     TSGFMVSESIGKF"
     gene            complement(4375..5124)
                     /locus_tag="P5335_07015"
     CDS             complement(4375..5124)
                     /locus_tag="P5335_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRZ74663.1"
                     /translation="MKTKFYLFFIVAILFQNCTKVEKKALIIGHRGAMGHETENTIAS
                     VKKALELGVDMIEIDVFLIKSGELVVFHDEILDSLTDTSGRIEEFTLEELQKVVVKGN
                     HQIPTLGEVIETVDKLVLLNIELKGKNTAEPTLKLLQKYYQKGWKKDDFVISSFLWDE
                     LTTVRKLDSEIPVAILTEENPTDALIMGKKLKAVAINPWWKTLTKENIELIQEEGFKI
                     YTYTVNELDDIKMVTELGVDGIFCNFPERLN"
     assembly_gap    5470..5569
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5666..7498)
                     /locus_tag="P5335_07020"
     CDS             complement(5666..7498)
                     /locus_tag="P5335_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HRZ74664.1"
                     /translation="MKNLFQFRFLFIYFLLSFGSILAQTATKAVPVFENGEAQIVPAF
                     KEADKWIREDLWVETTFDTDGDGKLDRMHVDVTRPSQTETEGLKLPVIYESSPYYSGV
                     APDVEGLFWDVKHELGETAKERVHAEVVRTGKRPIISNTLIGAWVPRGFIVVHSCSPG
                     TGLSQGSPTVGGKNESLAPKAVIDWLNGRANGFTSPDGDEKVTAYWSTGKVGMTGTSY
                     NGTIPLAAATTGVEGLEVIIPVAPNTSYYHYYRSNGLVRSPGGYLGEDIDVLYDFIHS
                     GDESKRPYNNKTIRDTEMKNGMDRISGDYNEFWAGRDYLNQMAPMKAAMLMSHGFNDW
                     NVMPEHSYRIYKKAKEMGLETSIFYHQDGHGGPPPFSMLNKWFTHYLFGVDNGVEKEE
                     NKAWIVRENDAMNNPTPYADYPNPASKPVSFYLTKGAPKMGGLTTSKSKKQGKETLVD
                     DYKLTGEILARAESSPNRVLYVTPTLTEDLHISGVPKITVKVASSKPAANLSVWLVSL
                     PWNTDSKAKITDNIITRGWADPQNHSSLTESKPLTPGKFYEVTFDLMPDDQIIKKGQQ
                     IGLMIFSSDNEYTLHPKSGTALTIDLDGTILTLPIVGGADSLKK"
     gene            complement(7620..>11219)
                     /locus_tag="P5335_07025"
     CDS             complement(7620..>11219)
                     /locus_tag="P5335_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRZ74665.1"
                     /translation="ATSFYDNQSLDTTSIVYSVFLTGNAANQLNDGATPSLSNFKKRL
                     EAASKNGTVLFLGDNVFPLHGFGENRTKAEEKISKQLELTHNFKGKVFFLPGETDWKN
                     GIPGILAQEDFITNYKKKTGQLIPPKACGLEFISLSDDMELVAIDSQWFKEDWDNHPE
                     INKGCSIKTREQLIEELDNHLLENQEKTILLALHHPLVSNGVYGGQYSLKQQLYPFDN
                     AVPLPIAGSFVNLFRKASGYNTEDIQNIRYSEMANQIKSIVQKYNHVVVVSGHEQNLQ
                     YISKDGIQQIISGSGAHFTPARAVNPKDFSYGGFGYAVLDLYANGASTISYFTTSNRK
                     EVLLFKQKLREPIVEEKKQTYPKQVENEVKKSIFGRPVSQKSAVYEYLWGKHYKNYYS
                     QIITSKTTLLDTLFGGLKPIGLDNESSNSSLILSDKKGKEYVMTPIEKDAASFFKSLA
                     YKNQNFKTQSGNEVTEDFLRDYFTTIHPFVPLVIPELSKAIGLRTTEPKLYFLPKQNK
                     LEVYNKDFGGAFYYISEKPQTAFKNNNEFGNAISIITTDEMLHLIRTDKNHVVDQENY
                     IRARLFDMLLGDWDREANKWQWGVYVEKNKTIYRPISTQRDQAFAKYDGAFLGLLKSI
                     PAMGQMHSYRYNLKNIKDFNQQAYALDLALLADADEKSWLKQAEFIQQNLSNDLINQA
                     FLKMPYELQDETTSKIKLHFRNRLKKLNQTAKEYHKLLEKKAIVVGTQKNDVIVITRL
                     SKGRTVVVVYNGLKTPTNIVFEKTFYKKNTKEIWLFGLEGNDEFSIEGKENNTIKIRL
                     IGGLNQDSYVVEYGKKITIYDFYANNKTITVDKKTTVLLSNNYETNTYNYKKPKFNTF
                     SGVPLMGFNPDDGLRLGARFDFVQHGFLGNPYSQKHSLLANYYFATSGYEFTYSGIVK
                     KAFGNFDLVIDAATTSSNFSQNFFGYGNETISMEEELGMDYNRVKMASFKVNPGLVWL
                     KNLGSTVIARLNLESYQVENTPERYISESGLDSRIFDFQNFAGIGFEYAFKNYDNPFL
                     PSLGLGFQAIGKWNVNVSDFSRQVPSLETSLNLVYKLTPDGQWLFETTLKGKSIFSNS
                     YEFYQAASLGGDTDLRGFRDNRFTGKSAYYQGSDLRYFIGQIRNPFAPMNYGAFIGFD
                     YGRVWFPSESSTKWHQSTGFGFWINSSNVIASTVSYFHSSDGGRLSFGMKFNF"
BASE COUNT         3558 a         2015 c         1868 g         3678 t
ORIGIN      
        1 tcttccggtc cttttaaagt aatgtaactt ttcgtttatt gttttacaaa tttctgaagt
       61 actttttgat tattgacggt gcttatttct actatataaa ctccttttgt ataacgagag
      121 atatctatag ctgtttgttc catgctcatg ttttgtttgc tataaacttc cttgcctaaa
      181 acgtcataaa taattattcc tttaatagaa tcaaaaccat ttttcaattg aatttgaatt
      241 aaatctttag caggattcgg gaataaaact aaatcagaat aagtaaaatc agccgtgctt
      301 aaactggcga caaattcggt ttcaaatgta ttggtaatga ttgccggatt aaaatcaaaa
      361 taaatttctg ccgtattagg tataatgtca ccaattgaaa ttccgggatt cgttttaatt
      421 ttaaaatgaa taaacccaat actggcattt tcattgacca cttttggcac taaatgaata
      481 ttctcaaact tccaaaccaa ctgattattt attctttcca atacataatt atgactagag
      541 gcaatcattc gcaaactttc gggatttaat tgtgcgtcta aaatatcttc caccctaaca
      601 aatgtagcat tggttgttcc ggtattttga aaacgaatgg tataatacaa gtaatcatct
      661 tgtgaaaatt gagctaacac aatttctttt ccgtgagctt catttttgtc attcgggtca
      721 taagacgcta caattggtcg ggttaaagaa gccgaattat ttgttgaatt ggcatctaaa
      781 gttgtagtgg aaatagaaac ggaattggtt actaaatcac caatggcaac ggttggaatt
      841 gcaggaacac tcatcgaaac attgatagtt cgggtttcca agggtaaaag atctgtataa
      901 tcaaaagtaa acccgttagc agaaggagtt gtgcctgttt gagagatacc cgtgatagtt
      961 aaaacaggag ctttatcaaa tgttaaggtt ccggaagccg gagcaattcc gttattagta
     1021 taaattagtt tgttttgata agtaaaccct gctgtaggtt ggttggtagc aactatagaa
     1081 acggctacat cattgtagga ggtggtgttt acaataggaa aataatacac atttgattcc
     1141 gtttcagaaa aaacaatatc atcataggaa ataggttgtg cataatatcc ggcatattca
     1201 gaataaacag cataacttaa atcataggta tttgttgtat tttcaggaaa aatataaaaa
     1261 acaccattgg aagatatatt tgaaactaca tcaccggtat tatttacttg ataattaaaa
     1321 ttaccattta caaaattttg ctcaccagaa tctttaattc cattattatt ggaatcaaga
     1381 aaagctatga gttgaatgtt atcttgacac tgatttccaa gagctaaact tccaccttca
     1441 ataaaaacag cagaatcaaa agcagaatcc tggtaatcac caaccgctaa ctttaaagaa
     1501 tattcgtggt ttggaataac agaggctatc gctgtcattg gcactgtata acctcgcata
     1561 ttcaaactac tagttggatt attgacatta taagtgctga aaaactgtgg atttacagaa
     1621 gcacacgacg tattatagat gttgtctcga atggtagttg ttgaaatcgg cacgttggtt
     1681 cccgggacaa gagcaatatt ttgggtttga ttggtggtta aatcagttaa aataaatccg
     1741 aatacatcgg aaaattgaca ttgataagca ccatattcat ttgaagcgaa aataaaatta
     1801 aagctcaaaa tatttgtagt gggaacaata tcaaacttta caaaggaaac atcattaatg
     1861 gtgccggttt gtccggctgc ttgcataatg gcagttagtt gtggatcgcc aatttgagaa
     1921 caattggacg tataattagc atcagtataa ggtccttgtg tcaaatttgc atttccgctt
     1981 ctgataatta atccttgctc aaatgggaaa ctactgtcat tagcgttaaa ataaccaaca
     2041 ccacatggac cttgggtaat tacattggag atagaagcac aatctgaatt taaaataaca
     2101 ttattcaaga tttgctcggg tgtaaatgaa gtggtattga ccgttacaaa tggaatggta
     2161 gtagtaccac aacttgtagt tgaaaagtta aacccggcag accaatcaga ggttatttgg
     2221 tcattacaaa ttgctcggac atagaaggta taagaggtgt cacaagttaa tgccgtaatg
     2281 acaaacggat ttacggaggc aataaaacct tgtgaatttg gatttggttc cggagatcca
     2341 aagggaacaa tataaatttc gtttgtgttt gtggctgatg gattaaacca acctaaagtt
     2401 gctccatttt cggtactatt aattgcgttt aggttgtttg gtgtttcaca actggtggaa
     2461 caattttgaa caagtaaaaa gaattcacca ccccaaaagc agcccgtgat aaaatcttca
     2521 attctcacaa aaataatttg attaggtgat gtattggtgt aattatatcc tatcggattc
     2581 acttgattta acatatcagc ataggttaaa taaaacgtga ctgacattgt atttggtgag
     2641 gcattgttcg ccgcataaat agtttgaacg gcttgattca aatcaaaaaa ggcaatcccg
     2701 tcattatcat catcacaggc aacagctact tcagtgccta tatttggaac tgaattaaca
     2761 acaattgtga aagtaactat ttcataatca gatgtattga tgttttgtaa acgagcaaat
     2821 agcgttacaa atcctatcat actattataa cttgtagggt ttgtgattgc attactgttt
     2881 tcttctgcat taattaaact ggtatgaaaa gaaatatcaa agtcatttgg gttttgacca
     2941 tttaataatg ttggaataat cgaagttaag tcaaaagttt cagttccatt ataatcagta
     3001 tcacaagcgg acaactgagt ttgtgaataa ctgaaaaaat tcacaaagca taaaaagaaa
     3061 atagtagaga gtaattttgt tttcattgat tttggttttg gtaaatcaaa tgtaattgat
     3121 ttttagtaaa ttatatagtt ttgaattaga atttcttagt attgaattag aatttaccaa
     3181 tactttcact caccataaac ccacttgtcc aggcattttg aaaattaaag ccacccgtaa
     3241 tcgcatcgat gttgactatt tctccggcaa aatatacttt aggaaggact ttgctttcca
     3301 tagttttaaa attgatttct tttaaatcaa taccaccggc ggtaacaaat tcttctttaa
     3361 acgtactttt tccgtttacc tgaaacttcc catttgtgag ttgtaaagct aaatgttgaa
     3421 gctgcttttt tgtcaaatca gcccatttgg tttctaaaga aatttcggaa gctaaaacca
     3481 aactttccca taatcggttt ggaaattcaa atggcgattt tttactgact acttttttgg
     3541 catgttccaa ttttaattcc tttaaatgac tttcggtttc ttccaaagta gcatcattca
     3601 accaattgac ttccaacata aattggtagt ttttagcaaa taattccctt gctccccaag
     3661 cagaaagtcg caaaataccc ggtccgctca ttccccaatg agtgattaaa agcggtcccg
     3721 atgcggaaag tttggagttt tgaactttta ctgtggcaaa agcagaaaca cccattaaat
     3781 cttttattcg agggtctttg atattaaaag taaataaaga gggaacaggc ggaacaatcg
     3841 tgtggcctaa attttccaat aattcccaaa ttttcggatt gcttccggta gtcatgacca
     3901 agtttttgca acgaaacgtt tggtgattgg tttccacttt ccaagtgtct tccgcttgat
     3961 aaagcgattg gacactttga ccggttagaa tttcgatttt gtgttttttg gcaacagtta
     4021 aaaaacaatc aataatggtt tgcgaagaat cggataccgg aaacattctg ccgtcgtctt
     4081 caatttttaa ttcgacaccg tggttggaaa accattctat cgtatcgccg gaacaaaatt
     4141 gatgaaacgg accacgcaat tctttttcgc cacgaggata aaattttact aagtcattag
     4201 ggacaaaaca ggcatgggtc acattacaac gaccgccacc ggaaacgctt acttttgtaa
     4261 gtacttcttt gccacgttcg agtattgcaa tgctaaattc aggatttttt tcagcaacgt
     4321 tgatagcggt aaaaaaaccg gcagcaccac caccaacgat aaggacgtca tacattagtt
     4381 caatctttcg gggaaattac aaaaaattcc atcgactccc aattcagtaa ccattttaat
     4441 atcgtcaagt tcgttgaccg tatacgtgta aattttaaat ccttcttctt gaataagttc
     4501 tatgttttct tttgtcaacg ttttccacca agggttaatg gcaaccgcct ttaatttttt
     4561 ccccattata agggcatctg tcggattttc ttcggtcaaa atggcaacag gaatttcact
     4621 atctaatttt cgaacagttg tcaattcatc ccacaaaaaa ctggaaatga caaaatcgtc
     4681 ttttttccaa cctttctgat agtatttttg aagtaatttt aaagtgggtt ctgcagtatt
     4741 ttttcctttc aattctatat ttaaaaggac taatttatca accgtttcga ttacttcacc
     4801 taaagttgga atttgatgat tgcccttaac caccactttt tgaagctctt ccaaagtaaa
     4861 ctcttcaatt cttcccgaag tatcagttaa actgtctagg atttcatcat gaaaaaccac
     4921 taattcacca cttttaatta aaaaaacatc aatttcaatc atatcgacgc ctaactctaa
     4981 cgctttttta acggaagcaa tagtgttctc tgtttcgtgc cccatagcac cacggtgacc
     5041 aataattaaa gcttttttct ccactttagt acaattttga aaaaggatag caactataaa
     5101 aaataaataa aattttgttt tcattgaaat aatttttatc aaaactcaaa aaattatttc
     5161 aattaattta agctaagtat caatattaag caaaaattaa tgttgttaac atgttgataa
     5221 tctgagaata gaatggaata taaaacaagc taagcattca aatcaacttt taaaacataa
     5281 aaacttaatg caacttaatt ccttaagttt aaaatcaact taattcaaaa ttaataacct
     5341 caaattcgag gtaaacctta aaaattaaac ttcattccaa acgaaatccg tccatgaaaa
     5401 agcaaaataa gtataatcaa aaagcgtgtt ttcatcggaa ttattttcaa gaacgtaaaa
     5461 atgaagtcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna atcaaaaagc
     5581 gtgttttcat cggaattatt ttcaagaacg taaaaatgaa gtcaacaaaa tcctcgttct
     5641 actggcatgc ttgtattaaa tttcattact tctttaaact atcagctcca cccacaatcg
     5701 gcaaagtcaa aattgtccca tccaaatcaa tcgttaatgc tgttcccgat tttggatgca
     5761 aagtatattc attatcactc gaaaaaatca tcaaaccaat ttgttgtcct tttttaataa
     5821 tttgatcatc tggcattaaa tcgaaagtta cctcataaaa ctttcctggt gttagcggtt
     5881 tactctccgt taaagaacta tgattttgag gatcagccca accacgagta ataatattat
     5941 ccgttatttt tgctttacta tctgtattcc acggaagtga aaccaaccaa accgataagt
     6001 ttgcagcagg tttgctactg gccactttta ccgtgatttt tggtacacct gaaatatgaa
     6061 gatcctcggt aagtgttggc gtaacatata acactcggtt tggtgatgat tcagcacggg
     6121 ctaagatttc tcctgtgagt ttataatcat caaccaatgt ttcttttcct tgttttttag
     6181 atttcgaagt agttaagcca cccattttag gagcaccttt tgttaaataa aaagaaactg
     6241 gttttgatgc tggattagga taatctgcat atggagttgg attattcatc gcatcgtttt
     6301 cacgaacaat ccaggctttg ttttcctctt tttcaacgcc attgtccaca ccaaataaat
     6361 aatgtgtaaa ccatttgttt aacatgctaa acggtggcgg cccaccatgt ccgtcttgat
     6421 gataaaaaat acttgtttcc agccccattt ctttggcttt cttataaatt cgatagctat
     6481 gctcaggcat aacattccaa tcattaaaac catgcgacat caacattgcg gctttcatcg
     6541 gagccatttg gttgaggtaa tcgcgtcccg cccaaaattc gttataatca cccgaaattc
     6601 tatccatgcc atttttcatt tccgtatctc gaatggtctt attgttataa ggccttttcg
     6661 attcatcacc actatgaata aaatcataca acacatcaat gtcttcaccc aaataaccac
     6721 caggcgaacg aactaatccg tttgaacgat aataatgata ataagatgta ttcggggcaa
     6781 caggaataat aacctctaat ccttctacac cagttgttgc agcagcaagc ggaatcgttc
     6841 cgttataaga tgttcctgtc atcccaactt taccggtact ccaataggcg gttacttttt
     6901 catcaccatc tggcgaagta aatccattcg ctcgaccgtt taaccaatca attactgctt
     6961 ttggagccaa tgattcgttt ttgccgccaa cggtaggcga gccttgtgac aaacctgttc
     7021 ctggtgaaca agaatgcacc acaataaatc ctctaggaac ccaagctcca atgagtgtat
     7081 ttgaaataat tgggcgttta ccggttcgga ccacttcggc atgaacacgt tctttcgccg
     7141 tttctcctaa ttcgtgcttc acatcccaaa acaaaccttc tacatcaggt gcaactcctg
     7201 aataataagg actcgattca taaatcacag gaagtttcaa accttccgtt tccgtttgtg
     7261 aaggtcgcgt aacgtccaca tgcattcggt ctagttttcc atcaccatcg gtgtcaaaag
     7321 ttgtttctac ccaaaggtct tctctaatcc atttgtcagc ctctttaaaa gcaggtacaa
     7381 tttgagcttc tccattttca aaaacaggaa ctgcttttgt agccgtttga gccaatatcg
     7441 aaccgaaaga aagtaagaag tagatgaata gaaaacggaa ttgaaataag tttttcatgt
     7501 tgagctgttt taggtaaatc attgtattga tgcttatcac aaatatagaa aataaatagg
     7561 cttgtaaaat ggcatgaaaa aacttaactc tattttcgtg gagcaggatt attttgatat
     7621 caaaaattaa acttcatccc aaatgataat cttccgccat cactcgaatg aaaataggaa
     7681 accgttgacg caattacatt actgctgtta atccaaaagc caaatccggt ggattgatgc
     7741 cattttgtag agctttcaga cggaaaccaa acacgaccat aatcaaaacc gataaatgca
     7801 ccgtaattca taggagcaaa aggatttctg atttgaccaa taaaataacg taaatctgaa
     7861 ccttgataat aggctgattt ccctgtaaaa cggttatctc taaatcccct caaatcagta
     7921 tctccgccca atgaagccgc ttgataaaac tcgtacgaat tactgaaaat tgatttccct
     7981 tttagggttg tttcaaaaag ccattgtcca tctggagtca atttatagac taaatttaat
     8041 gacgtttcta aggacggaac ttgtctggaa aaatcggaga catttacatt ccattttccg
     8101 atagcctgaa atcctaaccc taacgaaggt aaaaaaggat tgtcataatt tttaaaggca
     8161 tattcaaatc caattccggc aaaattttga aaatcaaaaa tgcgggaatc aagaccgctt
     8221 tcagaaatat accgttcggg agtattttca acttgatacg attctaaatt caaccgtgct
     8281 attacagtcg atccaagatt ttttaaccat actaaaccgg gatttacttt aaatgaagcc
     8341 attttcacac ggttgtaatc cattcctaat tcttcttcca tggaaatagt ttcgttgcca
     8401 taaccaaaaa agttttgaga aaaattggaa ctggttgttg cggcatcaat aacgaggtca
     8461 aaattaccaa atgctttttt tacgataccg gaataggtaa attcataacc gcttgtcgca
     8521 aaataataat tagccaataa actgtgtttt tgcgaataag gattgcctaa aaaaccatgt
     8581 tgaacaaaat caaatcgggc acctaatcgc aaaccatcat ccgggttgaa acccattaaa
     8641 ggaacaccgg aaaaagtatt gaattttggt tttttataat tgtacgtgtt tgtttcataa
     8701 ttatttgaaa gcaaaacggt tgtttttttg tccacagtta ttgttttgtt attggcataa
     8761 aagtcataaa ttgttatctt tttaccatat tcaacaacat acgaatcctg atttaatcca
     8821 ccaattaaac gaattttaat agtattgttt tcttttcctt caatactgaa ttcgtcgttt
     8881 ccttctaatc caaataacca aatttccttg gtattttttt tataaaaagt tttttcaaat
     8941 acaatgttgg tgggtgtctt taatccgtta taaaccacaa ccacagttct tccttttgat
     9001 aagcgggtga ttacgataac atcatttttt tgagtgccca ccacaattgc ttttttctca
     9061 agtagtttat gatattcctt agccgtctgg tttagctttt ttaaacgatt tctaaaatga
     9121 agctttattt tggaagtagt ttcatcctgc aattcataag gcatttttaa aaatgcttga
     9181 ttgattaaat cattagaaag attttgttga atgaattcag cttgtttaag ccaacttttt
     9241 tcatccgcat cagctaataa ggctaaatct aaggcatagg cttgctgatt gaagtctttg
     9301 atgtttttaa gattataacg atatgaatgc atttgaccca tcgccggaat gctttttaaa
     9361 agacctaaaa aagcaccatc atatttggca aatgcctgat cacgttgtgt agaaattgga
     9421 cgataaatgg ttttgttttt ttcaacataa acgccccatt gccatttgtt tgcctctctg
     9481 tcccaatcac ctaagagcat atcaaacagg cgagcccgaa tataattttc ttgatctact
     9541 acgtgatttt tatcagtacg aataaggtgt agcatttcat cagtggtaat aattgaaatt
     9601 gcatttccaa attcattatt gtttttaaaa gcggtttgag gtttttctga aatatagtaa
     9661 aaggcacctc caaaatcctt attatagacc tctaatttgt tttgttttgg caaaaagtac
     9721 agtttgggtt ctgttgtgcg taagcctatc gcttttgata actccgggat caccaatggg
     9781 acaaaagggt gaatggttgt aaaataatct cttaaaaaat cttctgtaac ttcattaccg
     9841 gattgagttt taaagttttg atttttataa gcaagtgatt tgaaaaagga ggcagcatct
     9901 ttttctatgg gggtcattac atattctttt ccttttttat cgcttaaaat aagtgaactg
     9961 tttgaacttt cattgtctaa tccaatcggt tttaaaccac caaacaacgt gtcaagcagt
    10021 gtggtttttg acgtaataat ttggctgtaa taatttttat aatgtttacc ccagaggtat
    10081 tcatatacgg cacttttttg acttaccggt cttccgaaaa ttgatttttt gacttcattt
    10141 tcaacttgtt taggatacgt ttgttttttt tcttctacaa taggttccct caatttttgt
    10201 ttaaaaagta atacctcttt tcgattagaa gtggtaaaat aggaaatagt agaagcaccg
    10261 tttgcataca aatctaaaac ggcataacca aaaccgccat agctgaaatc ttttggattt
    10321 accgctcttg ccggtgtaaa atgagcacct gaaccactga taatttgttg aatcccgtct
    10381 tttgaaatgt attgtaaatt ttgttcatgc ccggatacaa cgacaacatg attatatttt
    10441 tgaacaattg atttaatttg gttagccatt tcactataac gaatgttttg aatatcttcc
    10501 gtgttataac cactcgcttt tctgaataaa ttgacaaagc taccggcaat cggcaaagga
    10561 accgcattat cgaacggata aagttgttgt tttaaagaat attgacctcc ataaacacca
    10621 ttgcttacaa gcggatgatg cagagccaac aaaatcgttt tttcttggtt ttcgagtaga
    10681 tgattatcaa gttcttctat taattgttcc cttgttttaa tagaacagcc tttgttgatt
    10741 tcaggatgat tatcccaatc ttctttaaac cattgggaat cgatggcaac aagttccatg
    10801 tcgtcgctca aagaaataaa ttcaagacca catgcttttg gagggattaa ttgaccggtc
    10861 ttttttttgt agttggtaat aaaatcttct tgagctaaaa ttccgggaat accgtttttc
    10921 caatccgttt cgccgggtaa aaaaaatact tttcctttga aattatgtgt tagttctagt
    10981 tgtttactta ttttttcttc tgcttttgtt ctattttctc caaaaccatg taatggaaaa
    11041 acattatcac ctaaaaaaag aaccgtgccg ttttttgaag cggcttctag tcttttttta
    11101 aaattggata gggaaggagt agcaccatca tttaactgat tggcggcatt tccggtaaga
    11161 aaaacagaat aaacaatgga agtagtatca agggattgat tgtcgtaaaa cgaggtggc
//
LOCUS       DAOTCW010000070        11189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3753_length_11189_cov_36.456619, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000070 DAOTCW010000000
VERSION     DAOTCW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11189
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3753_length_11189_cov_36.456619"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="P5335_07030"
     CDS             complement(<1..1019)
                     /locus_tag="P5335_07030"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRZ74666.1"
                     /translation="MFNQQADYHIRMTKKSIALAILYLFFAFSCQKKVEAPLLAHYIP
                     EETTVTDSSQITIPLELNLKPVSKEFRNEKENEVSSYYKKYIGTTDFSGGFIVAKNGV
                     VIYEEYKGYANYAKKTVNEESTPLHLASVSKVLTATLVLKLVQENKIDLNEKVKTYLP
                     SFPYQNTTIKTLLNHRSGLPHYSRFEVLIKGWSRRTMLSNQDVLDILSKNKLALNTKN
                     DTHFDYCNTNYAILALVIEAVTGKSYPLVMQEKIFEPLKMNNSFVIDFNTQKNDVSQS
                     YKGNYVVHGWDQYDAIYGDKNIYSTPRDLVKFDLATYSNTFLRKDLMAEALKGYSYEK
                     RGIRN"
     gene            complement(1047..1478)
                     /locus_tag="P5335_07035"
     CDS             complement(1047..1478)
                     /locus_tag="P5335_07035"
                     /inference="COORDINATES: protein motif:HMM:NF018425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRZ74667.1"
                     /translation="MVFKSSIHLSSGLILTIIPTIMISVALMGLLFETPNTGIMIGSI
                     IFILIGSTFFWILLDTKYTIKENVLYYCSGPIRGKIDIQSIRKIKHQKGLYNECFLKP
                     SLDYNGLYIYYNKFDDIYLSPKDQDVFVNYLLKINPKIDLI"
     gene            complement(1489..>1782)
                     /locus_tag="P5335_07040"
     CDS             complement(1489..>1782)
                     /locus_tag="P5335_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRZ74668.1"
                     /translation="DSKTILTEIKPEKEITAEYRQQEFENLTTQDDHLMHIVGKVKDQ
                     KGNVYYKVKNSWGTTSGHDGFVYMSVPYLKLKAISVLVHQDALTPTTKKALGL"
     assembly_gap    1783..1882
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1883..2337)
                     /locus_tag="P5335_07045"
     CDS             complement(<1883..2337)
                     /locus_tag="P5335_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRZ74669.1"
                     /translation="MKNSICLLFLCFTSISFSQTYEFQTIKDIEATPVISQDITGTCW
                     SFSTTSFLEAEVIRLTGKKIDLSEMYNVRNTYPKKAENYVLRQGKAQFSEGGLAHDVI
                     NSVSQYGLVPNSVYTGLNGSNIKHNHTELQALLEGMLKVFVENPGKKLSP"
     gene            complement(2343..2864)
                     /locus_tag="P5335_07050"
     CDS             complement(2343..2864)
                     /locus_tag="P5335_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74670.1"
                     /translation="MKKYLSLVSILLLGFYVNAQVVKTEEFELTFPIQPTIDEQEIVT
                     EAGDTHLKMYQAMHEGAMILFVDATYPKELDVSTNKEVNKKVLKNSKNGSVNNFAGQM
                     GLKVNLISEVFVEYKSLLALRSIDGVGRYFAQVYTIAHLNKLYSILVFSETENGGKLI
                     LDNLVNSFTLLTI"
     gene            2925..3764
                     /locus_tag="P5335_07055"
     CDS             2925..3764
                     /locus_tag="P5335_07055"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HRZ74671.1"
                     /translation="MAEINWETAKEFEDITYKKCNGVARIAFNRPDVRNAFRPKTTSE
                     LYQAFYDAQEDTSIGVVLLSAEGPSSKDGVYSFCSGGDQRARGHQGYVGEDGMHRLNI
                     LEVQRLIRFMPKAVIAVVPGWAVGGGHSLHVVCDLTLASKEHAIFKQTDADVTSFDGG
                     YGSAYLAKMVGQKKAREIFFLGRNYSAQEAFEMGMVNAVVPHDELENTAYEWAQEILA
                     KSPTSIKMLKFAMNLTDDGMVGQQVFAGEATRLAYMTEEAKEGRNAFLEKRKPNFEKK
                     WLP"
     gene            3802..4404
                     /locus_tag="P5335_07060"
     CDS             3802..4404
                     /locus_tag="P5335_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRZ74672.1"
                     /translation="MEEFSNHSVSFDSLPKFESVTLTPLNKDYLNVVYIGNSIFSILI
                     AIGLLIFLYFNESIRTYIIPMIIILVCCIGLVFWVSTVGFKKKGFALREKDIIFRKGI
                     LATTTTIIPFNRIQHVALHEGVLSRMYQLSELQIYTAGGSSSDMHISGLQKEEAERIK
                     SFLLTKIVTPIDIFSNTEIELEKPNEIAVTDEEASEKSES"
     gene            4415..>4701
                     /locus_tag="P5335_07065"
     CDS             4415..>4701
                     /locus_tag="P5335_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74673.1"
                     /translation="MTFDFSKPQHQSVKGIAIMFVDTLQNIIRGLWVPLLLILYKFDW
                     SKMVFFGIGVFVLLLVIGIIAYLQYKNFTFFLDEEKEEFVIQKGVITKNKIT"
     assembly_gap    4702..4801
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4802..5518
                     /locus_tag="P5335_07070"
     CDS             <4802..5518
                     /locus_tag="P5335_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRZ74674.1"
                     /translation="QMVLQKKALAITYGLFAKKNTLLKPEKVQVTTYSQNFFQRKLNL
                     FDMKMKQASSTDETENDSKKSDIEVPGCNNNEREAILKMILKDLPQNEEVLNPNFRYL
                     IMTVLFGIIIPSAIFISLGVSVFEILQPYFPFVLVYVLFMAIVLFFKFKNYRLKLSRK
                     FIVIKSNAWDVEHQLLQPFKIQGITTKQYFWHKNADVVHITFHTAAGDVGFKFANFSL
                     LKPWINYWLYEVERTERSWM"
     gene            5563..6510
                     /gene="menA"
                     /locus_tag="P5335_07075"
     CDS             5563..6510
                     /gene="menA"
                     /locus_tag="P5335_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HRZ74675.1"
                     /translation="MKHWIEAARLRTLPLSVSGILVGSFYALAKPTEQELTPTNVFSW
                     KIFGLLLLTTIGLQVLSNFANDYGDGVKGTDNEDRIGPKRAIQSGAISPKAMKSAIII
                     TAFITFLLAVLLIYTAFHFKYFYYSLFFLALGIIAIASAIQYTVGTGAYGYKGYGDVF
                     VFVFFGLVSTYGSYFMFSKEFDWTLLLPATAIGFLSVGVLNLNNMRDEESDRKAGKNT
                     IVVKIGGAKAKQYHYFLLITAMILLLIFGFLQNFAIDQYLYVIVFLPIISHILRVKRN
                     VNPAELDPELKRLAISTFMVSMLLSLCLIYFLSDIIVNN"
     gene            6522..7199
                     /locus_tag="P5335_07080"
     CDS             6522..7199
                     /locus_tag="P5335_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRZ74676.1"
                     /translation="MKITFYGHASLGIEVSGKHILVDPFITGNPKAAHVDINLLQADY
                     ILLTHAHQDHILDVEAITKRTDALIVSNWEIATYFSNKGFKSHPMNHGGSWKFDFGSV
                     KFVHAVHSSSFPDGSYGGNPGGFVIEGEHKNIYISGDTALTMDMKLIPMRTKLDLAIL
                     PIGNNFTMDVEDAIIAADFVECDKILGVHFDTFGFIEINHEEAIKKFFNKGKDLMLLE
                     IGHFIEL"
     gene            7203..8246
                     /locus_tag="P5335_07085"
     CDS             7203..8246
                     /locus_tag="P5335_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HRZ74677.1"
                     /translation="MKATYQKYILNFKKPSGTSRGILTQKETWFIILDDNDKKGIGEC
                     GILRTLSIDDRPDYEEKLKWTCQNIHLGVDILWEKLIEFPSIQFGVEMAFQSLQSENP
                     FVLFPSAFTESSQTISINGLVWMGEELFMKQQIEEKIEQGFTCIKLKIGAIDFEKELS
                     LLRFIRQHFDEKTIEIRVDANGAFDSNEALDKLNQLADYKLHSIEQPIAKNNTDRMAE
                     LCRSTPFPIALDEELIGVFRMEEKVRLLQKIRPQFIILKPSLVGGFRGTKEWIDTAEQ
                     LQIKWWITSALESNIGLNAIAQWTFLQNNPMPQGLGTGGLYTNNFDCPLEVQKGMLLY
                     NNQLAWNTAFIDF"
     gene            8299..9069
                     /locus_tag="P5335_07090"
     CDS             8299..9069
                     /locus_tag="P5335_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRZ74678.1"
                     /translation="MFELENYLILLCLGAFIAGFVDAVVGGGGLIQTPLGFILLPNLH
                     VATVIGTLKIPSFTGTFLASTLYLKKVVIQWKSLLLMMVLAIPSAYAGSSLLNHISNE
                     FMKPFLLVVLTLLAIYSFVKKDFGLKSDKSHSPTQQLLYSIIISITIGFYDGFIGPGT
                     GIFFVMAFVSLLGYDFLQASANAKMVNLATNVGAISLFFIKGKIIWHIALPMAICNGL
                     GGFLGAKMAIKRGNNFVRIFFQILMVLMLLRFAWDVLT"
     gene            complement(9061..9540)
                     /locus_tag="P5335_07095"
     CDS             complement(9061..9540)
                     /locus_tag="P5335_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRZ74679.1"
                     /translation="MHTTHNLLLKFYDSFSKANAQSMAECYHPEATFKDPAFGTLNQQ
                     EVTAMWQMLVERSKGNLTIEFSNVSCTDYSGSVDWVATYDFSQTNRKVINFIQAQFEF
                     KDGLIFKHTDTFNLWKWSGMALGWKGYLFGWTPFMQNKIRSQAKTSLKNYMKRADLS"
     gene            complement(9524..10000)
                     /locus_tag="P5335_07100"
     CDS             complement(9524..10000)
                     /locus_tag="P5335_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74680.1"
                     /translation="MKKIVFLFCLFLLLGCKSKKGISSPSETEKPTVMKLSMSEVNPI
                     QKNRAYELGKRVLMTCNTSSFKPFTSEEATPEVLKNINKDKISLTCQNIIRGFGQFKD
                     IKLIEVFRLENEKITVFRYKCDYEKKYSIKELRVSVNDENKITAITTKDWKDAYNP"
     gene            complement(9997..10677)
                     /locus_tag="P5335_07105"
     CDS             complement(9997..10677)
                     /locus_tag="P5335_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013868773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRZ74681.1"
                     /translation="MGNFEKSVEKIQNIKVIDTSIPYEAYISLDLSIHSKQLSKFSPK
                     TAEDYSQFLNKYLDENKAKVAYGGYLEKRNLYERSTLFNEQSTEVRNIHIGLDLWIAA
                     GTSVLAALGGTVHSFQNNTNLGDYGPTIILQHTLNNCQFYTLYGHLSLESIALLHIGQ
                     PVKQGDKIAELGESSVNGDYAPHLHFQVIKNIGDYFGDYPGVCSEKDLDYFKENCPDP
                     NLLLKINS"
     gene            complement(10776..>11189)
                     /locus_tag="P5335_07110"
     CDS             complement(10776..>11189)
                     /locus_tag="P5335_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRZ74682.1"
                     /translation="GTDITIVSYGSTLRLVEQAAKELQEVGINCEIIDIQSLLPFDIN
                     HDIKKSLSQTNRLLVVDEDVPGGASAFILQQILDVQNGYDYLDSKPQTLASKAHRPAY
                     GTDGDYFSKPSSEDIFEKVYAMMNEVNPTDYPSLY"
BASE COUNT         3486 a         1648 c         2022 g         3833 t
ORIGIN      
        1 tagttacgaa tgccacgttt ttcataactg tagcctttta gggcttctgc cattaaatct
       61 tttcttaaaa aggtgttgga ataagtagct aaatcaaatt tcactaaatc tctcggggtt
      121 gaataaatgt ttttgtcacc ataaattgca tcgtattgat cccaaccgtg aacgacataa
      181 tttcctttgt aggattgact tacatcattt ttttgagtat tgaaatcaat tacaaacgaa
      241 ttgttcattt ttaaaggttc aaagattttc tcctgcataa ctaacggata ggattttccg
      301 gttaccgctt caataactaa agctaaaatg gcatagtttg tattgcaata atcaaaatgg
      361 gtatcattct ttgtatttaa ggccagttta tttttgctta atatatcaag tacatcttga
      421 ttggaaagca ttgttcttcg actccaacct ttaatgagta cttcaaagcg agaataatgc
      481 ggtaaaccac ttctgtgatt caacaacgtt ttgattgttg tgttttgata tggaaagcta
      541 ggtaaatagg tttttacctt ttcgtttaaa tcgattttat tttcttgaac cagttttaaa
      601 accagcgttg ctgttaaaac tttactcaca gatgctaaat gaagaggagt tgattcttcg
      661 ttgacagttt ttttagcata attagcgtag cctttatact cttcataaat aactactcca
      721 tttttagcca ctatgaatcc tccactaaag tcggttgtgc caatatattt tttataatag
      781 gaggaaactt cattttcttt ttcatttcta aattctttag aaacgggctt caaatttaat
      841 tctaaaggaa tggtaatttg agaagaatct gttactgtgg tttcttccgg gatgtaatga
      901 gctaagagtg gtgcttccac ttttttctga caggaaaaag cgaaaaataa ataaagaatt
      961 gctaaagcaa tcgatttttt agtcatgcgt atgtggtaat ctgcttgttg gttaaacaaa
     1021 actaaccttt ttaaattaaa gtcttcttaa attaagtcaa ttttaggatt aattttcaac
     1081 aagtaattaa caaatacatc ttggtctttt ggagataaat aaatgtcatc aaatttattg
     1141 taatatatat aaaggccatt ataatctaat gaaggtttta aaaagcattc attgtataac
     1201 cctttttgat gtttaatttt ccggatagat tggatgtcaa ttttaccacg aatgggtccg
     1261 gaacaataat ataaaacatt ctctttaatg gtgtatttag tgtctaataa tatccaaaaa
     1321 aaggttgaac caatgagtat gaaaataatt gaaccaatca tgattccggt atttggtgtt
     1381 tcaaataata accccattaa agctacggaa atcataatgg ttggtataat tgtcagtatc
     1441 aatccactgg acaaatgaat ggaagacttg aaaaccatac ttgttttttt ataaacctaa
     1501 tgcttttttg gtagttggtg tcaatgcatc ttgatgaact aatacagaaa tggctttaag
     1561 ttttaaataa ggcacactca tgtaaacaaa accatcatgt ccggaagttg ttccccaaga
     1621 gttttttact ttataataaa cgtttccttt ttgatctttc acttttccta caatgtgcat
     1681 caaatggtca tcttgagtcg ttaggttttc aaattcctgt tgacgatatt cagcggtgat
     1741 ttctttttca ggtttaattt ctgttaaaat ggttttactg tcnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnggtgaaag ttttttaccc ggattttcaa caaatacttt
     1921 taacatgcct tctaataaag cttgtaattc ggtgtggtta tgtttgatat tcgaaccgtt
     1981 taaaccggtg taaacagaat ttggtacgag cccgtattga ctgacactat tgataacatc
     2041 atgagctaaa ccgccttcgc tgaactgtgc tttgccttgt cttaatacat aattttcggc
     2101 tttcttaggg taggtattac gaacgttgta catttcagac aagtcgattt ttttaccggt
     2161 taaccgaata acttcggctt ctagaaagga agttgtggaa aaagaccagc aggttcctgt
     2221 aatatcctga gaaataacag gcgttgcttc gatatctttg atggtttgaa attcataagt
     2281 ttgagaaaaa cttatactgg taaagcataa aaataaaagg caaattgaat ttttcatttt
     2341 ttttagatag taagaagagt aaaagaattg actaagttat caagaattag tttcccaccg
     2401 ttttcagttt cactaaaaac aagaatggaa tagagtttgt ttaaatgtgc gattgtataa
     2461 acctgagcaa aatagcggcc aacaccatca atagaacgta aagcaagcaa agatttatat
     2521 tctacaaaaa cttcggaaat caaatttact tttaatccca tttgtccggc aaaattgtta
     2581 acgctaccgt tttttgagtt tttcaaaact tttttgttta cctctttatt ggtggaaaca
     2641 tctaattctt tggggtaagt ggcatctaca aataaaatca ttgcaccttc atgcattgct
     2701 tgatacattt ttaaatgagt atcaccggct tcggtaacaa tttcctgttc atcaattgtt
     2761 ggttgtattg ggaaagttaa ttcaaattct tctgttttta caacttgtgc attgacataa
     2821 aatcctaaaa gtaaaatact tactaaggaa aggtattttt tcatgattat ttttatagat
     2881 ttcaaaagtt aaaaatagta tttttacacg taaatcgtat tgttatggca gaaataaatt
     2941 gggaaacagc aaaagagttt gaagatatca cgtataaaaa gtgcaatggc gtagcccgaa
     3001 ttgcctttaa tcgcccggat gttcggaatg ctttccgtcc gaaaaccact tcagaattat
     3061 accaagcttt ttatgatgct caggaagata cctcgatagg tgttgttttg ttgtcagcag
     3121 aaggaccttc gtctaaagat ggtgtctatt ctttttgtag tggaggcgat caacgggcaa
     3181 gaggtcatca aggttatgtt ggagaagacg gcatgcatcg tttaaatatt ttagaggttc
     3241 aacgcttaat tcgttttatg ccaaaggcgg tgattgctgt agttcccgga tgggctgtag
     3301 gtggcggtca tagtttgcat gttgtttgtg acttaacgtt ggccagcaaa gaacacgcta
     3361 tttttaaaca gacagatgcc gatgtgacta gttttgatgg agggtatggt tctgcctatt
     3421 tagccaaaat ggttggacag aaaaaagcca gggaaatttt ctttttaggg cgtaattatt
     3481 cagctcagga agcttttgag atgggaatgg ttaatgccgt tgttcctcat gatgaattgg
     3541 aaaatacggc ttatgaatgg gctcaggaaa ttttagcaaa atcaccaacg tcaattaaaa
     3601 tgctcaaatt tgctatgaat ttaaccgatg atggaatggt aggtcaacaa gtttttgcag
     3661 gcgaagcaac tcgattggcc tatatgactg aggaagcgaa agaaggaaga aatgcatttt
     3721 tggaaaaaag aaaacctaat tttgaaaaga aatggcttcc gtaattttta aactaaatag
     3781 atttaattaa aaacacctac tttggaagaa tttagcaatc attctgtttc ttttgactcc
     3841 ttaccaaaat ttgagtcggt aaccttaacg cctttgaata aagattactt gaatgtggtt
     3901 tatattggta attctatatt ctctatactc atcgcaattg ggttacttat atttttatat
     3961 ttcaatgaaa gtattcgaac ttatattatt ccgatgataa ttattttagt ttgttgcatc
     4021 ggattagttt tttgggtttc aaccgtggga ttcaaaaaaa aaggattcgc cttgcgggaa
     4081 aaagatatta tctttagaaa aggcatactg gcaacaacca ctacaattat tccatttaac
     4141 cgaatccaac atgtagcttt acatgaaggg gtattgtcca gaatgtatca gttgagtgag
     4201 ttacaaattt atacagccgg aggaagcagt agtgatatgc atatttcggg tttacaaaaa
     4261 gaagaagcag aaagaataaa atctttttta ctgactaaaa ttgtaactcc aattgatatc
     4321 ttttcaaaca ccgaaattga gttggaaaaa cctaatgaaa tagcagtaac tgacgaagaa
     4381 gcgagtgaaa aatctgaatc gtaaaactga aacaatgaca ttcgatttta gcaaaccaca
     4441 gcaccaatcg gttaaaggaa tagccatcat gtttgtagac actttgcaaa acattatacg
     4501 tggattgtgg gttccattgt tgttgatact ttacaaattt gattggagca aaatggtgtt
     4561 tttcggtatc ggtgtctttg tacttctatt ggttatagga attattgctt atttacaata
     4621 taaaaatttc actttttttt tagatgagga aaaggaagaa tttgtgattc aaaaaggagt
     4681 gattactaaa aataaaataa cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 ncaaatggtg cttcaaaaga aagccttggc aataacatac ggattatttg ctaagaagaa
     4861 cacgctgctc aagccggaga aagtgcaggt aacgacatat agtcagaatt tttttcaacg
     4921 aaagttgaat ttatttgata tgaaaatgaa acaagcttcc tcaactgatg aaacagaaaa
     4981 tgactcgaaa aaatcggata ttgaggttcc aggttgcaat aataatgaac gggaggcaat
     5041 tttgaaaatg attttgaaag acctgcccca aaacgaagaa gtattaaatc cgaattttag
     5101 gtatttaatt atgactgttt tgtttggaat aattataccg agtgccattt ttataagttt
     5161 gggtgtatca gtttttgaaa ttctacagcc ttattttcca tttgttttgg tttatgtttt
     5221 gtttatggct attgtattgt tttttaaatt taaaaattac cggttaaagc tttcccgtaa
     5281 atttattgtt ataaaaagca atgcttggga cgttgaacat caattattac agcctttcaa
     5341 aatacaagga ataactacaa aacaatattt ttggcataaa aatgcagatg tagttcatat
     5401 aacttttcat acggcagcag gcgatgtagg atttaaattt gctaattttt ctttgttaaa
     5461 accttggatt aattattggc tttatgaagt agaacgcacc gagcgaagtt ggatgtaaat
     5521 acacaataat tctattacct ttgtacttta aataaaacga taatgaaaca ttggattgaa
     5581 gcagctagat taagaacatt accgttatcg gtatccggaa ttttagtggg aagtttctat
     5641 gctttggcaa aaccaacgga acaagagctg actcccacaa atgtttttag ttggaaaata
     5701 ttcggattat tgcttttgac aacaattggg ttgcaagtat tatccaattt tgcaaacgat
     5761 tatggtgatg gtgtaaaagg aaccgataat gaagacagaa ttggtccaaa acgagccata
     5821 caaagcggag ctatttcacc taaagcaatg aaaagtgcta taataattac agcttttata
     5881 acttttttgt tggcagtttt attgatttat actgcttttc attttaaata tttttattat
     5941 tctttatttt tcctagcatt aggaataatt gcgattgcct ccgcgattca atatacagtt
     6001 ggcaccggag cttatggata taaaggctat ggtgacgttt ttgtttttgt gttttttgga
     6061 ctggtgagta cctatggttc ttattttatg ttttctaagg agtttgattg gacactttta
     6121 ttgccggcga cagcaattgg gtttttaagc gtaggggtgc tcaacttgaa caatatgcgg
     6181 gatgaggaat ctgaccgaaa agcaggaaaa aataccatag tggtaaaaat tggaggtgca
     6241 aaagcgaaac aataccatta ttttctgctc attaccgcaa tgattttgtt attaatcttc
     6301 ggatttcttc aaaattttgc tattgatcaa tatttatatg taattgtttt tttaccgata
     6361 atcagtcata tacttcgcgt taaacgaaac gtgaacccag cagaattaga ccctgaactt
     6421 aaaagattag ccattagtac ttttatggtt tccatgcttt tgtcgttatg tttgatatat
     6481 tttttatctg atattattgt aaacaactaa attagttagt gatgaaaatt acattttatg
     6541 gtcatgcttc tctaggaatt gaagttagtg gaaagcatat cttagtagat ccatttatta
     6601 caggcaaccc taaagctgct catgttgata taaatttgct gcaagccgac tatattttac
     6661 tcacacatgc tcatcaagat catattttgg atgtagaagc tattactaaa agaactgatg
     6721 ctttaattgt ttctaactgg gaaattgcta cctattttag caacaaaggc tttaaaagcc
     6781 atcctatgaa tcatggcgga agttggaagt ttgattttgg ttctgtaaaa tttgttcatg
     6841 ctgttcattc gagttctttt cctgatggaa gttatggcgg aaatccgggt ggttttgtaa
     6901 ttgagggaga acataagaat atttatattt ccggagatac agcgttgact atggatatga
     6961 aactcattcc gatgcgaaca aaattagatt tagctatttt gccgattgga aataatttca
     7021 cgatggatgt agaagatgct attattgccg ctgattttgt agaatgtgat aaaatcttgg
     7081 gggtacattt tgatacattt ggatttatag aaatcaacca tgaagaagcg attaaaaaat
     7141 tctttaataa gggcaaagat ttaatgcttt tagaaattgg ccattttatt gaattgtaac
     7201 ccttgaaggc aacttatcaa aaatatattt taaacttcaa aaaaccctcc ggaacttcca
     7261 gaggaatact tactcaaaaa gaaacctggt ttatcatttt agatgataat gataaaaagg
     7321 gaataggtga gtgcggtatt ttaagaacat tgagcattga cgatcgtccg gattatgaag
     7381 aaaagttaaa atggacctgt caaaatattc atttaggagt tgatatactt tgggaaaaat
     7441 tgattgaatt tccgtctatt caattcggag tggaaatggc ttttcaatcg ttgcaaagtg
     7501 aaaatccatt tgtcttattt ccttctgctt ttactgaatc atctcaaact atttcaatta
     7561 acggactagt atggatgggt gaagaattgt ttatgaaaca acaaattgaa gaaaaaatcg
     7621 agcaaggttt tacttgcatc aaacttaaaa taggagcgat agattttgaa aaagagttga
     7681 gtttattgcg atttattcgt caacattttg atgaaaaaac gattgaaatt cgtgtagatg
     7741 ccaatggggc tttcgattca aacgaagctt tagataaatt aaatcaatta gctgattata
     7801 aattacatag tattgagcaa cctattgcca aaaacaatac tgacaggatg gcagagctgt
     7861 gtagaagcac accttttcct attgctttag atgaggaatt gataggggtt tttagaatgg
     7921 aagaaaaggt taggttacta caaaaaatta gacctcaatt tatcatttta aagcctagtt
     7981 tagttggtgg gtttcgagga acaaaagaat ggattgatac agcggagcaa cttcaaatta
     8041 agtggtggat tacttctgct ttagaaagta atattggatt gaatgctatt gcacaatgga
     8101 cttttttaca aaataatcca atgcctcaag gtttaggaac aggcggatta tataccaata
     8161 attttgattg tccgttggaa gttcaaaaag gaatgctgct ttataacaat caattagcat
     8221 ggaatacagc ttttattgat ttttgagagt catttataat ttgaatattt tgctattaat
     8281 atgattaatt aaacccaaat gtttgagctt gaaaattatt taatcttatt gtgtttgggt
     8341 gcttttattg ccggttttgt tgatgctgtt gttggtggtg gcggtttaat acaaacgcca
     8401 ttgggcttta ttttgttgcc aaacttacac gtagcaaccg taattggaac tttgaaaatt
     8461 ccttctttta cgggaacttt tttagcttcg acactgtatc ttaaaaaagt ggtcattcaa
     8521 tggaaatcgt tattacttat gatggtttta gcaatacctt cggcttatgc gggttcctcc
     8581 ttattgaatc atataagcaa tgaatttatg aaaccatttt tattagtggt tttaacctta
     8641 ttagcgatat attcttttgt taaaaaggat tttggactta aatcggataa atctcattca
     8701 ccaacacaac aactgctgta tagtatcata ataagcataa ctattgggtt ttatgatgga
     8761 tttattggtc ccggaactgg tattttcttt gtgatggcct ttgtgagttt gttaggttat
     8821 gatttcttgc aggcttcagc aaatgcaaaa atggttaatc tggctacaaa tgttggagcg
     8881 atttcactct tttttatcaa agggaaaatt atttggcata ttgccttgcc aatggctatt
     8941 tgtaacggat taggcggttt tttaggagct aaaatggcaa tcaaaagagg aaataatttt
     9001 gttcgtatat ttttccaaat tctgatggta ttgatgttgc ttcgttttgc ttgggatgtt
     9061 ttaacttaaa tccgctcgtt tcatataatt ttttaaagac gttttagcct gacttctgat
     9121 tttgttttgc ataaaaggtg tccaaccaaa taaataacct ttccatccta atgccatgcc
     9181 gctccatttc caaagattaa aggtgtcggt gtgtttaaag attaaaccat ctttgaattc
     9241 aaattgtgct tgaataaaat tgatgacttt gcggtttgtt tggctgaaat cataggtggc
     9301 aacccaatca acagaaccac tataatccgt gcagctaaca ttggaaaatt caatagttaa
     9361 atttcctttg ctgcgttcaa ccagcatttg ccacatggcg gtcacttctt gttggttcag
     9421 cgtaccgaaa gccgggtctt taaaagtcgc ttccggatga taacattctg ccatagattg
     9481 ggcatttgcc ttgctgaatg aatcgtaaaa ctttaagagt aaattatggg ttgtatgcat
     9541 ctttccaatc ttttgtggta atggctgtta ttttattttc atcgtttaca gatacgcgaa
     9601 gttcctttat actgtatttc ttttcgtaat cacatttata acgaaagacg gtaatttttt
     9661 cgttttctag cctaaaaact tctattaatt taatgtcttt aaattgtcca aaaccacgaa
     9721 taatattttg acatgttaat gaaattttgt ctttattaat attttttaaa acttccggtg
     9781 ttgcttcttc acttgtaaag ggcttgaaac tagaggtgtt gcaagtcatc aaaacgcgtt
     9841 tgcctaattc ataggctctg tttttttgta ttggatttac ttcactcatg gaaagcttca
     9901 tcactgttgg tttctcagtt tctgaaggcg aagaaattcc ttttttcgat ttacagccga
     9961 gtaataaaaa cagacaaaaa aggaaaacaa tttttttcat gaattaattt taagtaataa
    10021 attcggatca gggcaatttt ctttaaaata gtccaagtcc ttttcactgc aaacaccggg
    10081 atagtctcca aaataatctc caatattttt tatgacttga aaatgtaaat gaggagcata
    10141 atcaccgtta actgaggatt cacctaactc agcgatttta tcaccttgtt taacaggttg
    10201 accaatatgt aacaaagcta tactttctaa cgaaagatgg ccataaagcg tataaaattg
    10261 acaattattt aacgtatgtt gtaaaataat tgtcggtccg taatcgccca aattggtatt
    10321 gttttgaaaa ctatgaacag tacctcctaa tgctgctaaa acggatgttc ctgcagcaat
    10381 ccataggtct aggccaatat gaatgtttcg tacttcagta gattgttcat taaaaagagt
    10441 actgcgttcg tataaatttc gtttttcaag atatccgcca taagctacct ttgctttgtt
    10501 ttcatctagg tatttgttga gaaattggct gtaatcttca gcggttttag gtgaaaattt
    10561 cgacagttgt ttgctgtgta tggataaatc caatgaaata taagcctcat aaggaatgga
    10621 agtatcgatg actttgatat tttgtatttt ttctacactt ttttcaaaat ttcccatctg
    10681 aatcaattaa actgtttgtt aaaatattga ttttaaactt tggcaacaaa aaaagccgtt
    10741 gattaaacga cttttcgatt tattaattag ttattttaat ataaactggg ataatcagtt
    10801 ggattcacct cattcatcat agcgtagact ttttcaaaaa tatcttcaga ggaaggtttt
    10861 gaaaaataat caccatctgt tccgtaagcc ggacgatgag cttttgaggc taacgtttga
    10921 ggcttactgt caagataatc atagccgttt tgaacatcaa gaatttgctg taaaatgaaa
    10981 gcggatgctc caccgggaac atcttcatca accactaata aacgattggt ttgagataaa
    11041 cttttcttaa tgtcatggtt tatgtcaaaa ggcaaaagtg attgaatatc aattatttca
    11101 caatttatac caacctcttg taattcttta gctgcttgtt ctaccaaacg taaagtggaa
    11161 ccataagata cgattgttat atctgttcc
//
LOCUS       DAOTCW010000071        11170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3764_length_11170_cov_46.068286, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000071 DAOTCW010000000
VERSION     DAOTCW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11170
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3764_length_11170_cov_46.068286"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="P5335_07115"
     CDS             complement(<1..1587)
                     /locus_tag="P5335_07115"
                     /inference="COORDINATES: protein motif:HMM:NF015347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HRZ74683.1"
                     /translation="MKIKFTLAIFLLSFASFAQQPFITTWKSVFGSTNITFGTVTTGP
                     VDYSWESLPPAAPSSGSGTFQGPIVNITGLPAGERIRLTIQPQNFKRIFADGFSFTIT
                     EINQWGSVEWISMENAFNIQDLFFPNGIQLVNATDIPNLSNVTSMANMFAGSSQLNGP
                     FNINFWNISTVTNLSGMFKGCLNFNQALSLWNTSNVTDMSSMFEGARSYNQNIGNWNT
                     SSVTNMSKMFKDAFDFNRNIGNWNTVNVTDMSEMFNGDINSGPQYIFNQNIGNWNVSN
                     VTNMSAMFKGAIFFNQNIGNWDVSNVLNMSDMFNQAFSFNQNIGNWNTSNVTNMARMF
                     RSDVVPFTVTFPTVSLFNNGGSTAIQNWNTSNVTDMSGMFFRAENFNYNLGNWVLNPA
                     VNLEEMLDRCGLDCKNYSQTLIAWNGNPNTPNNKILGATFMEYGPEAISSINNLIFNK
                     GWGFSGHDFLTVVPAFDLDTSYCEGATIPALPTVSEDGIPGSWSPGLNSSQTTTYTFV
                     PEVGQCAATTSITITITPFPI"
     gene            complement(1777..1971)
                     /locus_tag="P5335_07120"
                     /pseudo
     CDS             complement(1777..1971)
                     /locus_tag="P5335_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
     gene            complement(2083..2499)
                     /locus_tag="P5335_07125"
     CDS             complement(2083..2499)
                     /locus_tag="P5335_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74684.1"
                     /translation="MKKFIVLILFVLISCTKSEESSNNLLLSKIVGNWKVVGYYDDIE
                     DSETGSNYHLVENGPVTTYKPDKTFDHIYSSTEVYYGNYSISMDSILTMNYSTTASGE
                     PYLYKTKIFLLNDAVLEFASETSETWGMRFEKINTP"
     gene            complement(2521..>4250)
                     /locus_tag="P5335_07130"
     CDS             complement(2521..>4250)
                     /locus_tag="P5335_07130"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HRZ74685.1"
                     /translation="LFVYLFICLANLNAQECGFVPSENYQTYDEVLYNRDSSIIENQC
                     INICFRIARDNNGSNAAIDPSMIPQIMTQLNSKFNPHGIFFNQIGSYDYINNTNFNNY
                     ISGSIPENIPNCLNIYFIKIFQSNPGWGGIATFGNLRVTVRGINALDGSLTHEVGHAL
                     NLLHTFQCTSSSTGFNASCAESPFSSFECNIRGDKVCDTPADYTNLFYNNNNNPYGLS
                     NYNPDIKNIMSYWPDKQHFTPGQGNRMINSIIGASQLQSIRSYECDKIVGPSKLCKIF
                     INGNYRLLLTPIGTTTYNWNVSGSLQINGPSTNSTVSISRLSTAGAVTSTVTVTLNGT
                     ITKTKTIRTRCIFGILRITGVYDWISKDYGNMGLVIPIDPEDEDPEDLVVKYLWEIKE
                     YSSEANTSSNESSKPFFVGASLNEPLIYVSTSNQAIVNWGNFSSSYLITCHEITQSGE
                     KYLISENYVDVGDPKNNPCFKNNIQSVIAPNPVRNGIINVTVNKPENSTPCNYKDLDE
                     PQFFNSELDRINNSITIFDLQGNEIYRNVFGNNEFKIDNVNLISGNNYVVNLYTNEGG
                     FNQQVIIAE"
     assembly_gap    4251..4350
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4579..5058)
                     /gene="rnhA"
                     /locus_tag="P5335_07135"
     CDS             complement(4579..5058)
                     /gene="rnhA"
                     /locus_tag="P5335_07135"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRZ74686.1"
                     /translation="MSHEVHIYTDGAAKGNPGPGGYGVVMELVGSPYKKEFYEGFRYT
                     TNNRMELLAVVVGLEKLKNPNTSVLVTSDSKYVVDAVEKRWVFQWEKKGFAGKKNPDL
                     WMRFLKIYRNHKVDFQWIKGHNNHPQNERCDELAVYASNLSNLSVDAFYEKEEGKLF"
     gene            complement(5055..5645)
                     /locus_tag="P5335_07140"
     CDS             complement(5055..5645)
                     /locus_tag="P5335_07140"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRZ74687.1"
                     /translation="MQNIVVFASGAGSNAENIIRYFKNNTSKMVIAVFSNNAIAKVIE
                     KAKNLDVQTIVFNKDELYGDFVLKNLKELNPDLIVLAGFLLKFPEHIIIEYPNKIINI
                     HPALLPKYGGKGMYGKYVHQAVLDNKENETGITIHYVNEQFDEGGIIFQKATSISDCQ
                     STDEIANKVHELEQRFFPEIIDQLLNGNLNSQNTTL"
     gene            5820..6056
                     /locus_tag="P5335_07145"
     CDS             5820..6056
                     /locus_tag="P5335_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRZ74688.1"
                     /translation="MSDIASRVKAIIVDKLGVDENEVVTEASFTNDLGADSLDTVELI
                     MEFEKEFDIQIPDDQAENIATVGQAISYIEEAKK"
     gene            6234..7487
                     /gene="fabF"
                     /locus_tag="P5335_07150"
     CDS             6234..7487
                     /gene="fabF"
                     /locus_tag="P5335_07150"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRZ74689.1"
                     /translation="MELKRVVVTGLGALTPIGNNIQEYWNALINGVSGAAPITYYDTE
                     KHKTKFACEIKNFNIEDFIDRKEARRMDKFAQYAIVASDEAIKDAGITADNVDKYRVG
                     VIWGAGIGGLETFQEEVLYYAKGDGTPKFNPFFIPKMIADIAPAHISMRNGYMGPNYT
                     TVSACASSANAMIDAFNYIRLGLCDVIISGGSEAAVTIAGMGGFNSMHALSTRNESPE
                     TASRPFDVTRDGFVLGEGAGALVLEEYEHAKARGAKIYCEVGGGGMSSDAYHLTAPHP
                     EGIGVIAVMNNCLRDANMKPEEVEHINTHGTSTPLGDVAELKAISAVFGEHAKNININ
                     STKSMTGHLLGAAGGIEAIASILAIEHGIIPPTINHVHIDENINPELNLTLNKAQKRD
                     IKVAMSNTFGFGGHNACVLFKKLEE"
     gene            7494..8237
                     /locus_tag="P5335_07155"
     CDS             7494..8237
                     /locus_tag="P5335_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HRZ74690.1"
                     /translation="MSLFKKIFTKSRPLEGGIFFDAIHKILGFKPNEIEYYRKAFTHR
                     SSNKLDLKGNPINYERLEFLGDAMLSAVIAGHLFNVVPSGDEGYLTKMRSKIVSREHL
                     NELGKDLNLIQLVDSKVPFQHFGDNIHGNIFEALVGAIFLDRGYVYCEKFIHKRVILP
                     YVDIAKLEGKVISYKSLVIEWCQKEKKPFNYDVFDDNGNDGQRFFGVKLSIDNKVIAK
                     ARATSKKKAEEIASKRAYFAFQEKMDTKF"
     gene            8347..8832
                     /locus_tag="P5335_07160"
     CDS             8347..8832
                     /locus_tag="P5335_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HRZ74691.1"
                     /translation="MAIHKLFIDDFEEVDYQLIAIHTTLDDFRIAYYINQKLPILLSK
                     SKKDILIETDKQKTAFTHFNFEDEKKSVFWTLIENKKEISLLQKNNTVNLFNAQQEFT
                     NTYYLLPELKKVDYFLKIEGDEKQINTISITNKLKTIDQVTTVYPVKKSQLKSKNNLI
                     F"
     gene            8839..10269
                     /gene="pyk"
                     /locus_tag="P5335_07165"
     CDS             8839..10269
                     /gene="pyk"
                     /locus_tag="P5335_07165"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRZ74692.1"
                     /translation="MPTNKKTKIVATLGPACSSKEIIKNMIDAGVNVFRINFSHADYA
                     DVKERIDIIRGLNEEFGYSTAILADLQGPKLRVGVMKEDVVVSKGDIITFQTAEDIPG
                     TAERVYMNYKEFPRDVKPGEKILLDDGKLMFEALETNGTTEVVCRVIQGGPLKSKKGV
                     NLPNTKVSLPALTKKDVKDALFAIENNVDWIALSFVRTPKDLEELQDMIAKHSEHKIP
                     IIAKIEKPEAVENIDKIVAFCDGLMVARGDLGVEVPAEEVPLIQKKLIHRAKTARIPV
                     IVATQMMETMITSLTPTRAEVNDVANSVMDGADAVMLSGETSVGNYPVEVIETMTKII
                     KSVEDSPLIQVPHNPPHVRTKRFITKSICFHAAVMANEIKAKAISTLTNSGYTAFQIS
                     AWRPSAHILVFTSNKRILTQLNLLWGVRSFYYDKFASTDDTVEDVNMIAKAKGFVDKG
                     DMVINLAAMPIADKGMVNTLRVSDIP"
     gene            10306..11076
                     /locus_tag="P5335_07170"
     CDS             10306..11076
                     /locus_tag="P5335_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRZ74693.1"
                     /translation="MTSNNPFFKSKSFNKPQTVEVDEQGNRSVLIDYNETMTVSGTIN
                     KSLIMLLLLVASATLTWMMTASGYNPLPFLIGGAVVGLILVLVASFKPHLSGYLAPGY
                     ALFEGLFIGALSALFESMYPGIVIQAVGATFTTFLVCFMLYKYKVVTVTEKFKSIVIG
                     ATITIAIYYLITWLLSMFTSFQPVHYGNSLMSIGISVLVIVVAALNLFLDFDQIEKGA
                     EKQLPKYMEWFGAMGLMITLVWLYVEFLRLFSKISSRD"
BASE COUNT         3762 a         1838 c         1812 g         3658 t
ORIGIN      
        1 gatggggaat ggtgtaattg ttatggtaat agaagtggta gcggcacatt gaccaacttc
       61 cggcacaaat gtgtaggttg ttgtttggct tgaatttaac cccggtgacc aagagccggg
      121 tataccatct tcagaaacag ttggaagtgc gggtattgtt gccccttcac agtaactagt
      181 atctaaatca aatgcaggaa caacagtcag aaaatcatgt ccggaaaaac cccacccttt
      241 attaaaaatt aaattattaa tggatgaaat ggcttccggt ccatattcca taaaagtagc
      301 tcctaaaatt ttattgttgg gtgtattcgg atttccattc catgcaatta aagtttgaga
      361 ataattctta cagtctaacc cgcagcgatc aagcatctct tccaaattaa ctgccggatt
      421 taagacccaa ttccccaaat tataattgaa attttctgct ctaaaaaaca ttccggacat
      481 atcagttacg tttgaggtat tccaattttg aatcgcggtg cttcctccgt tattaaacaa
      541 tgaaacagtt ggaaatgtta cagtaaatgg tacaacatca cttctaaaca ttcgtgccat
      601 attggttacg tttgaagtat tccaattgcc tatgttttgg ttgaatgaaa atgcttgatt
      661 aaacatatct gacatattta aaacatttga aacatcccaa ttacctatat tttgattgaa
      721 aaaaatcgct cctttaaaca tagctgacat gtttgtaaca tttgaaacat tccaattacc
      781 tatgttttga ttaaatatat attgagggcc agaattaata tcaccattaa acatttcaga
      841 catatcagta acatttaccg tgttccaatt cccaatgttt cgattaaaat caaaagcatc
      901 tttaaacatt ttactcatat tggttacgct acttgtattc caattgccta tgttttgatt
      961 ataacttcga gccccttcaa acatgctcga catatctgtt acatttgagg tgttccataa
     1021 gcttaaagct tgattaaaat tcagacaccc tttgaacatt cctgaaagat tggtaacagt
     1081 tgaaatattc caaaagttga tgttgaatgg tccatttaat tgtgaacttc ccgcaaacat
     1141 attagccatg cttgttacat tagataaatt cggaatatcg gttgcgttga ccaattgaat
     1201 accattcggg aaaaacaaat cttgaatgtt aaaggcattt tccatactaa tccattctac
     1261 agatccccat tggtttatct cggtaatagt aaatgaaaac ccatccgcaa atatcctttt
     1321 aaaattttga ggttgaatag ttaaacgaat tctttcccca gcaggtaaac ctgtaatatt
     1381 tacaattgga ccctggaatg tgcctgagcc tgaagagggt gcagcaggag gtaatgattc
     1441 ccaagaataa tctaccggtc ctgttgtaac tgttccaaaa gtaatattgg tagagccaaa
     1501 aacggatttc catgttgtga taaagggttg ttgagcaaaa gaggcaaaac tcaataaaaa
     1561 gatagctagg gtaaatttta ttttcatggc ttttttatag taatattaac tataaaagag
     1621 taaattatgc ggtaatttaa ctcgcttttc acaattgttt gacctaaaaa tcaatacaat
     1681 ttcattttaa aaaaaataaa acttagttta aaaaaaaaat tagatgtcat tctatatcta
     1741 aaaaactgaa aagccttgtg gttattggtg tttttatcaa aacaatttcc cttcctcctt
     1801 ttcataaaaa gcatccacag aaagatttgg taaattagaa gcataaacag ccaattcatc
     1861 acaacgttca ttttgaggat gattgttatg cccttttatc cattgaaaat caactttatg
     1921 attgcgatag atttttaaaa aacgcatcca taaatcgggg ttttctattt tacaagaata
     1981 aatgcaacgt cataaaatga actgcatcat tgtctgaaca attgattaat gatatcaaag
     2041 ttgataataa aatatttttt ttacttttaa aattatttta tcttatggtg tattaatttt
     2101 ttcaaaccgc ataccccaag tttcagatgt ttcagacgca aattcaagta cagcatcatt
     2161 caataaaaaa attttagtct tatataaata aggttctcca gatgctgtgg tagagtaatt
     2221 cattgtcaat atagaatcca ttgaaatgga ataattgcca taataaactt cagtagaact
     2281 gtaaatatga tcaaaagtct tgtcaggttt atatgttgtt acgggtccat tttcaactaa
     2341 atggtagttt gaacctgttt cagaatcttc aatgtcatca taatatccaa caaccttcca
     2401 atttccaact attttgctta ataaaagatt attcgaactt tcttcacttt tggtacaact
     2461 aatcaaaaca aacaaaatta atactataaa ttttttcata taataaattt taaaacttaa
     2521 ttattctgct attattactt gttgattaaa acctccttcg ttagtatata aatttacaac
     2581 atagttgttc ccgcttatta aatttacgtt gtcaatttta aattcattat ttccgaaaac
     2641 gtttctataa atttcattcc cctgtaaatc aaaaatagta atagaattgt ttattctatc
     2701 tagttcacta ttaaaaaatt gaggttcgtc aagatctttg taattacaag gtgttgagtt
     2761 ttcaggttta tttaccgtaa catttattat tccgtttctt acaggattag gagcaataac
     2821 agattggata ttgtttttaa aacatgggtt gttttttggg tcaccaacat caacataatt
     2881 ttcactaatt aaatatttct ctcctgattg agtgatttca tggcaagtaa ttaaataact
     2941 actagagaaa ttaccccaat taacaatggc ttgattagaa gtacttacat aaattaaggg
     3001 ttcatttaat gaagcaccaa caaaaaaagg tttactactt tcattactac ttgtgtttgc
     3061 ttcactagaa tattctttta tttcccacaa atattttact actaaatctt ctggatcttc
     3121 atcttctgga tcaataggta ttactaatcc catatttcca tagtctttac ttatccaatc
     3181 atatactcca gttattctaa gaattccaaa aatacatcta gttctaatag tttttgtttt
     3241 ggtaattgtt ccatttaagg tgactgtaac tgtagatgtt acagcaccag cagttgataa
     3301 cctagatata gagacagttg agtttgtaga gggtccattt atttgtaggc tcccgctcac
     3361 attccaatta taagttgttg ttccaattgg tgtcaataaa agtctgtagt taccatttat
     3421 aaaaatttta cataattttg aaggacctac aattttatca cattcatatg aacgaatact
     3481 ctgtaattga ctagctccaa taattgaatt tatcattcga ttaccttgac caggagtgaa
     3541 gtgttgtttg tctggccaat aagacataat gttttttata tctggattgt aattactcaa
     3601 cccatatggg ttattattgt tattataaaa taaatttgta taatctgctg gagtatcaca
     3661 aactttatct cctctaatat tacattcaaa agaactaaaa ggactttctg cacaacttgc
     3721 attaaaacca gttgaagatg aagtacattg aaaagtatgt aataagttta aagcatgacc
     3781 aacttcatgg gttaatgaac catctaaagc atttattcct ctgactgtta ctcttagatt
     3841 tccaaaagtt gcaattcctc cccatccagg gtttgattga aatattttta taaaataaat
     3901 atttaggcaa tttggaatgt tttcaggaat gctccctgag atataattat taaagtttgt
     3961 attgtttatg taatcatatg aacctatttg attaaaaaat atcccatgag ggttaaattt
     4021 tgaattcaat tgtgtcatta tttgtgggat catgcttgga tcaatagccg catttgagcc
     4081 attattatct ctagcaattc taaaacatat atttatacat tgattttcaa ttatagatga
     4141 atctctatta taaagaactt catcataagt ttgataattt tcagacggta caaatccgca
     4201 ctcttgtgca tttaagtttg ccaaacaaat aaacaaataa acaaataaac nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaacaaataa acaaataaac aaataaactg
     4381 taatgatttt tcataaataa acaaggaatt attgattaat tctttgctaa tttagtaatt
     4441 aaatcattaa taaatagctc ttttttgtaa ttttacatta aatataattt ttgctccgcc
     4501 acataatgtc ttgttaaaag catattggtc aaaaacatat catgcttttt gcttaaaatc
     4561 cactataagt caactaaatt aaaacaactt cccttcctcc ttttcataaa aagcatccac
     4621 agaaaggttc gataaattag aagcataaac agccaattca tcgcaccgtt cattttgagg
     4681 atgattattg tgccctttta tccattgaaa atcaacttta tgattgcgat agatttttaa
     4741 aaaccgcatc cataaatcgg gatttttctt tccggcaaaa cccttttttt cccattgaaa
     4801 aacccatctt ttttcaacgg catccaccac atattttgaa tcagatgtga ccaaaactga
     4861 cgtattgggg ttttttagtt tttctaaacc tacaaccaca gccaataatt ccattctatt
     4921 gttggtggta tacctgaaac cttcgtagaa ctctttttta tagggtgagc caactaattc
     4981 cataaccaca ccatagccgc ctggcccggg atttcccttg gctgctccgt ctgtgtagat
     5041 gtgaacttcg tggctcataa agttgtattt tgagaattta gattaccgtt caaaagttgg
     5101 tcaattattt ctggaaaaaa acgttgttct aattcatgaa ctttgttcgc tatttcgtcc
     5161 gttgactgac aatctgaaat tgaagtcgct ttttgaaaga ttattcctcc ttcatcgaat
     5221 tgttcattta cataatgaat agtgattcct gtttcatttt ctttattgtc caaaacagcc
     5281 tgatgaacat atttcccata cattcctttt ccaccgtatt tcggtaataa agcgggatga
     5341 atgtttataa ttttatttgg atattctata ataatatgct ccggaaattt gagtaaaaag
     5401 cccgccaaaa caattaaatc agggtttaac tcttttaaat ttttaagcac aaaatcgccg
     5461 taaagctcgt ctttattgaa tacaattgtt tgaacatcaa gattttttgc tttctcaatc
     5521 actttggcaa tagcattgtt tgagaaaaca gcgattacca tttttgaggt gttatttttg
     5581 aaataacgaa tgatgttttc cgcattggaa cccgctccag aggcaaaaac cacaatgttc
     5641 tgcatatttt ctgataaatt tattagacaa aaaaacgaat taaatatgag attttatacc
     5701 gattgagcta ctattgtgaa ataaattttt taaattcgta gtcacattta ttaactaaaa
     5761 tgttgtttta aaataaagtt ttttattttt gccaacaatt aaactaaaat ttaaagatta
     5821 tgtcagacat tgcatcaaga gtaaaagcga ttatcgtaga caaattaggt gttgacgaga
     5881 atgaagttgt aacagaagca agcttcacaa acgatttagg agctgattca ttagacactg
     5941 ttgagctaat tatggaattc gaaaaagaat ttgacattca aattccggac gaccaagctg
     6001 aaaacattgc tactgtaggt caagcaattt cttacatcga agaagcaaaa aaataataac
     6061 tacaaacatc ccgttctgtc tttttcagaa cgggtgttat tatttttact aaaaaattaa
     6121 tttactgatt gactttcaat caaacacata tacaaaaaat cacccctgtt ttttactttt
     6181 caataaaaat cagggttttt ttgtttatat tcgtaccaat aaatttttaa gaaatggagt
     6241 taaaacgagt agttgttacc ggtttaggtg cattaacacc cattggaaat aatattcagg
     6301 aatattggaa cgcccttatc aacggcgtaa gtggagctgc tccaattacc tattatgata
     6361 ctgaaaaaca caaaacaaag tttgcttgtg aaataaaaaa tttcaacatt gaagatttta
     6421 ttgatcgcaa agaagcaaga agaatggata aatttgctca atatgccatt gttgccagcg
     6481 atgaagcgat caaagatgct ggtattactg ctgacaatgt agataaatac cgcgttggag
     6541 ttatttgggg agcaggaatt ggaggattag aaacctttca agaagaagtt ttatattatg
     6601 caaaaggtga tggaactcca aaattcaatc ccttttttat tccaaaaatg attgctgata
     6661 ttgccccggc tcatatttcc atgcgaaatg gctacatggg cccaaattat acaactgttt
     6721 cagcttgtgc ttcatcggca aacgcaatga tagatgcttt taattacatc cgtttaggtt
     6781 tatgtgatgt aattatttca ggcggctcag aggccgctgt aactattgcc ggaatgggtg
     6841 gatttaattc aatgcacgct ttgtcaacta gaaatgaaag cccggaaaca gcctccaggc
     6901 cttttgatgt tacccgtgat ggttttgttt tgggtgaagg tgccggagct ttagttttag
     6961 aagaatatga acacgcaaaa gcaagaggag caaaaatata ttgtgaagtt ggtggtggag
     7021 gaatgtcctc tgatgcctat catttaacgg ctccacatcc ggaaggaatt ggcgtaattg
     7081 cggtaatgaa taattgcttg cgagatgcca atatgaaacc ggaagaagta gagcacatca
     7141 atacacacgg aacctcaaca cctttaggtg atgtcgctga attaaaagca atcagtgctg
     7201 tttttggcga gcatgctaaa aacatcaata tcaattctac aaaatcaatg accggtcatt
     7261 tattaggagc cgctggtgga attgaagcta ttgcttctat tttggcaatt gaacatggaa
     7321 ttattcctcc aacgattaac catgttcaca tagatgagaa tattaatccg gaactgaatt
     7381 taacattaaa taaagcacaa aaaagagaca taaaggtagc gatgagcaat acttttggtt
     7441 ttggaggaca caatgcctgc gttttattta aaaaattgga agagtaaact taaatgagtc
     7501 tttttaaaaa aatatttacc aaatcccgtc ctcttgaagg cgggattttt tttgatgcaa
     7561 ttcataaaat tcttggattt aaaccgaatg aaatcgaata ttacagaaaa gcttttacgc
     7621 accgctcctc aaataaatta gatttaaagg ggaatccgat caattatgaa aggttagagt
     7681 ttttaggcga cgcaatgctt agtgcagtaa ttgcaggaca tttatttaat gttgttcctt
     7741 ccggtgatga aggttactta accaaaatgc gttcaaaaat tgtgagtaga gagcacttaa
     7801 atgaactggg aaaggactta aacttaattc aattagtgga tagcaaagtg ccttttcaac
     7861 attttggcga caacatccac ggaaatattt tcgaagcatt agttggtgct atattcttgg
     7921 acagaggtta tgtttattgt gaaaaattca tccataaaag agtgatttta ccctatgtcg
     7981 atattgcaaa attagaagga aaagtaatta gttataaaag tttggtaatt gaatggtgtc
     8041 aaaaagaaaa aaaaccattt aattacgacg tttttgatga taatggaaat gatggccaac
     8101 gcttttttgg cgttaaattg agcattgata ataaagtaat tgcgaaggca agagcgacct
     8161 ctaaaaagaa agcagaagaa attgcttcaa aacgtgctta ttttgcgttt caggaaaaaa
     8221 tggatactaa attttaagaa tctcctaaaa ctatatcgtt ttcgttaata aattatcgtt
     8281 aagttccgaa tttcaaattc gaatctactc caaaaatact atatttacat cttgttttaa
     8341 tgagaaatgg ctattcacaa attattcatc gatgacttcg aagaggttga ttaccagctt
     8401 atcgctattc atacaacctt agatgatttt cgaattgcat actatatcaa ccaaaaatta
     8461 cctattctgt taagcaaatc taaaaaagac atcttgattg aaacagataa acaaaaaacc
     8521 gcatttactc attttaattt tgaagatgaa aagaaatcgg ttttttggac attaattgaa
     8581 aacaaaaaag aaatcagcct tcttcaaaaa aacaatactg ttaacctatt caatgcacag
     8641 caggaattta caaacaccta ttacttatta cctgaattaa aaaaagtaga ttatttcctc
     8701 aaaattgaag gggatgaaaa acaaataaat accatatcaa ttacaaataa actaaaaact
     8761 attgaccaag tcaccactgt ttatcctgtg aaaaaaagtc aactaaaatc taaaaacaat
     8821 ttaatttttt aaataattat gccaaccaac aaaaaaacta aaattgttgc caccctaggg
     8881 ccagcatgca gttcaaaaga aatcattaaa aacatgattg atgccggagt aaacgtattc
     8941 cgaatcaatt tttcccatgc tgactatgcc gatgtgaaag aacgaattga tattattcgt
     9001 ggtttaaatg aagaatttgg atactcaact gctattttag ccgacctaca aggtcctaaa
     9061 ttaagagtag gtgtgatgaa agaagacgtt gtagtcagca aaggcgacat tatcaccttt
     9121 caaactgcgg aagatatacc ggggactgca gaacgggttt acatgaatta caaagaattt
     9181 ccaagagacg ttaagcccgg agaaaaaata cttttagacg atggaaagct gatgtttgaa
     9241 gctttggaaa caaacggaac aacagaagtg gtttgtcgtg ttattcaagg tggtccattg
     9301 aaatctaaaa aaggggttaa cctcccaaat acaaaagttt ctttaccggc tttaactaaa
     9361 aaagacgtga aagatgcttt atttgccatt gaaaacaatg tagattggat tgctctatcc
     9421 tttgtgagaa caccaaaaga tttggaagaa ttacaagata tgattgccaa acattcagaa
     9481 cataaaattc caatcatagc aaaaatagaa aaaccggaag ccgttgaaaa tatcgataaa
     9541 attgttgcct tttgtgatgg tttaatggtt gcccgtggtg accttggtgt tgaagttccg
     9601 gcagaggaag ttccgttaat tcagaaaaaa ttgattcatc gtgccaaaac agccagaatt
     9661 cctgttattg tagctactca aatgatggaa actatgatta cgagtttaac gcccaccaga
     9721 gctgaagtaa atgacgtagc caactctgtc atggatggtg ccgatgccgt tatgctttca
     9781 ggcgaaactt ctgttggaaa ttatccggtt gaagttattg aaaccatgac caaaatcatc
     9841 aaatcagtag aagacagtcc gcttattcaa gttccgcaca acccgcctca tgttcgcaca
     9901 aaacgattca ttacaaaatc catttgtttt catgctgcag tcatggcaaa tgaaattaaa
     9961 gccaaagcta tttctacatt gactaatagt ggttataccg cttttcaaat ttcggcttgg
    10021 cgaccaagtg cccatatttt agtgtttact tctaacaaac gaattttaac acaattaaat
    10081 ttattatggg gagttcgctc tttttattat gacaaatttg ccagcactga tgatacagtg
    10141 gaagatgtaa atatgattgc caaagcaaaa gggtttgtag ataaaggcga catggtcatc
    10201 aatttagccg ccatgccaat tgcggataaa ggaatggtga ataccttacg cgtatctgat
    10261 attccataat tttatttata tttgaaagac aaaaaattaa aaaatatgac ttcaaacaat
    10321 ccatttttta aaagcaaatc attcaataaa cctcaaacag tagaggttga tgaacaagga
    10381 aatcgttcgg ttctaattga ttacaatgaa accatgactg tttcaggaac tattaataaa
    10441 agtttaataa tgcttttatt attggttgcc tcggctacct taacttggat gatgaccgct
    10501 agtggctata atcccttgcc attcttaatt ggtggtgctg ttgttggact aattttagtt
    10561 cttgtagcat cctttaaacc gcatctttcc ggctatttag ctccgggtta tgctttattt
    10621 gaaggattgt ttattggagc tttatctgct cttttcgaat ccatgtatcc aggaatcgta
    10681 attcaggctg taggagcaac ttttacaacc tttttagtgt gtttcatgtt atataaatac
    10741 aaagtggtta ccgtcacgga gaaattcaaa tctattgtaa ttggagccac catcaccatt
    10801 gctatttact atcttattac gtggttgtta agcatgttta caagcttcca accggtacat
    10861 tatggaaatt ccttaatgag tattggaatt agtgtcttag ttatcgtagt ggctgcctta
    10921 aatttatttc ttgattttga tcaaattgaa aaaggagctg aaaaacaatt gcctaaatac
    10981 atggaatggt ttggagcaat gggattaatg ataaccttag tttggttata tgttgaattc
    11041 cttcgattgt tctcaaaaat atcaagtaga gattaattac aattcataca aaaaaaagga
    11101 gcttttaaag ctcctttttt tatgccgctt tctcaaaagc aataaaatta atcaattcac
    11161 cgttttggtt
//
LOCUS       DAOTCW010000072        10850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3918_length_10850_cov_44.357388, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000072 DAOTCW010000000
VERSION     DAOTCW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10850
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3918_length_10850_cov_44.357388"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3049
                     /locus_tag="P5335_07175"
     CDS             <1..>3049
                     /locus_tag="P5335_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74694.1"
                     /translation="FEVIALPAGSPAPTAATPGVLATSNPFVVTGLDPSTAYDFYVRA
                     LCTGPNPSSLWAGPATATTLPTCPAPINLIVIGADTNTATISWTNIAPATLFEVVVQP
                     VGSGIPTVAGVITSQNPYTATGLSAGFYEFYVRAICSSTDLSAWAGPQTFFILASLPG
                     CAGVDIDLQTSTPGVLNLCPGESCVDLSATFFETGDTTTYNVTGIPFTPPFPFTGGIP
                     TSVNIDDRWSNVIDLPFNFCFFGQNYTKALVGSNGVISFDIQGEVPGGAQIPNGYCAW
                     PFTQTIPNAAFPIKAAIYGPYQDIDPSVTTPPAQPSINYQVLGTAPCRVLVVNFSEVA
                     QFSCQNSVPLQTSQIVLYETSNAVEVYVQNRVPCLTWNGGRAVIGIQNQAGTQAHFPP
                     DRNTGTWSASNEAWRFTPAGPSNVTFSWLKDGEFYSNDLSINVCVSETTSMTAQAIYT
                     ACGGEQTITTDTVLLNVVGTELPVQDDVTVCVTEGYELPTLLVGNYFTEPGGQGTQLN
                     AGDIITTDQTIYVYLVINGTNGDCTSEGSFNVITTTEIVPEFDPIPNICLGGTAPALP
                     PTSLNGINGLWTPSTIDTSVEGTINLLFEPFGSEGCVTSYPTQVTILPFDDVLPIEDV
                     VACNSYILPDLAAGNYYTESGGLGLEVPSGTEVIESTTLYVYTANGMCSDEESFTITI
                     NSVTGDVLPNVSACDNYTLPTLLANNNYFTEPGGLGTQLNAGDVITTTQTIYIYTESG
                     TTPNCVAESSFVVTIGSLVLDPIADVVACDSYILPVLTQGNYFTETGGTGTQLNAGDT
                     IVTNQLIYVYAVDGTCLDEISFTVTINTISVDVIADQTACDSYTLPALSANNSYYTLA
                     GGLGTQLNAGDAITTTQTIFIYAQSGTTPNCVAESSFVVTIGSLVLDPIADVVACDSY
                     ILPALPQGNYFTATGGTGTQLNAGDSIESTQLIYVYAADGTCTDEISFNVTINTVSVD
                     VIVDQTVCDSYILPTLSANNTYYTQAGGLGTQLNVGDLITSNQT"
     assembly_gap    3050..3149
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3150..3839
                     /locus_tag="P5335_07180"
     CDS             <3150..3839
                     /locus_tag="P5335_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRZ74695.1"
                     /translation="ADVIPNATECLADGGFILPALSAGNNYYTGSGGTGQQLAAGDVI
                     TSDQTIYVFAQTATTPNCFNETSFTVTIVNVTAQELSDVEECGKYTLPELNSGNAYYT
                     GQNGTGTQLQPGSDVTTSQVIYVYATVGGCSAQTEFEVTINSCLIQKGISPNNDGSND
                     FFDLVNFNVSKLEIFNRYGTKVYSKNNYEDEWYGQSDKGDELPDGTYYYVIYFTEGTN
                     TTGWIYINRVR"
     gene            3910..4827
                     /locus_tag="P5335_07185"
     CDS             3910..4827
                     /locus_tag="P5335_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRZ74696.1"
                     /translation="MKKIYFALLIVLVSIADVSAQQDPHYTQYMYNMNVINPAYAGSK
                     ENLSFGLLYRKQWVEIEDAPTTFTLSGSSPVGKNVGVGLSLISDEIGPVTEKNVYGDF
                     SYTLNLGGEHRLALGLKAGVTFHDVDFTKIIPTLPDPADGVFANNINNTYFNFGTGIF
                     YYTDKYYLAFSVPNMINSKHLDYDGREYGSETTHYFFTGGYVFDLSANVKFKPFFMVK
                     SAFSAPTSLDLSTNFLFNEKFEIGATYRLEDSFGAMVNYAISPSLRIGYAYDHIVSDL
                     NITTPASHEIILLFDLNFPKKVSRSSRFF"
     gene            4848..6728
                     /locus_tag="P5335_07190"
     CDS             4848..6728
                     /locus_tag="P5335_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRZ74697.1"
                     /translation="MKKVYIFLLSIVMLSITPLSAQNKDTKKADKLFKQFDYFEAADE
                     YLKLAENGKGDGYVHKQLADSYYNMFNAAESSKWYARAVETPQDAETYYRYAQMLKAQ
                     GKYEEANKQMQQFATKAPNDQRAKDFKANPNYLPKLLDKQKQFDVKALELNSDKSDFG
                     AVLHENSLYFASARNGAKKTYGWTDEPYLDIYKANYNVDGTITNAEPVAEVNSKWHDG
                     PVTLSADGNTMYFASESFKEKSFEKDKVNNTRLKVSQVNLFKATNVNGKWSNITILPF
                     CSNAYSTGNPYLSKDGKTLYFSSNMPGSLGGNDVWKVAVNQDGGFGTPENLGAKINTE
                     GDESFPYISDDNKMFYFASSGRQGFGGYDVFVYDMAKGESVNMGKPVNSEKDDFAFTF
                     SKDKNIGFLSSNRAGVDNIYLATPVCGVEVTTIVTDAKTGAILVNAKVAIVDDKKNVI
                     ATEMSNAKGEVTYYVECDKNYTVQASKDGYESNTFPVAASKGPQKRVDAPLNPIDVIV
                     TETEVILNPIFFEFDKSNITQEGAFELDKLIQVMQNNPEMVIMAKSHTDNRGSDVYNL
                     SLSDRRAKSTVQYIISKGISKDRISGKGMGETEPKADCKEACTEEQHASNRRSEFLIV
                     KK"
     gene            complement(6786..8201)
                     /locus_tag="P5335_07195"
     CDS             complement(6786..8201)
                     /locus_tag="P5335_07195"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRZ74698.1"
                     /translation="MKSTRIFFLIALLFGSLFFIRCNTEDDITIPINPSTPTDVFNLD
                     KVPEITLELTEEQWNLLLTNYDLNPKNETKVVAKYRYSLDGNTIELDSIGLKLRGNTS
                     RRRPEGAIGQLHTSSNPDWHHCHFGVDFNKNIKTQRFQGLKKLNLKWFKDDATYVREI
                     YCYDLFRRYGIWTAPRASYCRVSIKVEGDVAPAYYGVYVMLESVDEDFINYRKENWTQ
                     SIGFMWKGGWAGYQNADFVQTESIGVEEVDLNPSNSLYYAYDLKTRENELDGAKTQLI
                     SFINNLNSKTGSAFKTWISQQMDVDLFLKTYAVNIMVGMWDDYWVNANNFYFYMAPDG
                     KVYFIPYDYDNTLGTSMLMPNSGTQNPLNWGPASGRPLITKILAIPEYQTKFKTYIKE
                     LADSNNDYFDPTKSRNRIASWQFKIGPYISNDTGEDMLIEDKPAFWGNAPFYRLSSGN
                     NFGGNSGPANYFSSRTSSIPW"
     gene            complement(8249..9325)
                     /gene="arsB"
                     /locus_tag="P5335_07200"
     CDS             complement(8249..9325)
                     /gene="arsB"
                     /locus_tag="P5335_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRZ74699.1"
                     /translation="MSSNNCAPVVERKKLGFLDRFLTLWIFLAMAIGVSIGYFIPSSS
                     GFINSFSSGTTNIPLAVGLILMMYPPLAKVKYEQMGEVFKNTKVLGASLFLNWIVGPI
                     LMFLLALLFLNGYPEYMIGVILIGLARCIAMVVVWNDLADGNREYAAGLIALNSIFQV
                     VLYSVYAYIFITILPPYFGYDSFEVNISIGQIAESVGIYLGIPFALGVISRYSLIALK
                     GTEWFNNKYVPFISPITLIALLFTIVLMFSLKGELIVQIPLDVVRIAIPLVIYFTIMF
                     VLSFFVGKYFGADYSKSTAIAFTATGNNFELAIAVAIGVFGINSGQAFAGVIGPLVEV
                     PALIALVNLAFWFRKKYYTKEQTS"
     gene            complement(9329..9946)
                     /locus_tag="P5335_07205"
     CDS             complement(9329..9946)
                     /locus_tag="P5335_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase"
                     /protein_id="HRZ74700.1"
                     /translation="MKLYPKIEKYLSEISFDSITADRKQTLQSLIDYIQIKVTNKQDV
                     RLNLICTHNSRRSHLAQVWAQAAATYYEIPNVYCYSGGTEATAMFPMAAKTLENAGFQ
                     IQTIAEGTNPIYAIKFAENAHPILGFSKTYDDAFNPQSEFAAIMTCSQADGGCPFIAG
                     AEKRIPITFEDPKAFDNTPQQAIKYEERSLQIATEMSYVFAQIKK"
     gene            complement(9983..10453)
                     /locus_tag="P5335_07210"
     CDS             complement(9983..10453)
                     /locus_tag="P5335_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6428 family protein"
                     /protein_id="HRZ74701.1"
                     /translation="MKLSQIKNVLSTVENVTFQLPNGTFAPEHFHVTEVGLITKNFID
                     CGGTVRKETVVNFQLWDANDYDHRLKPQKLANIIALSERVLGIEDHEIEVEYQGETIG
                     KYDLDFNGQSFVLVNKKTACLAQDQCGVPTQKPKVRLSDMNNEPSCCTPGGSCC"
     gene            complement(10507..10839)
                     /locus_tag="P5335_07215"
     CDS             complement(10507..10839)
                     /locus_tag="P5335_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRZ74702.1"
                     /translation="MGITKSDYFTDQQNELAILAKAIGHPARIAIIEYLLKVDSCICG
                     DIVNELPLAQPTVSQHLKELKNAGLIKGNVEGVSICYCLNETGFDKIKTFFETVNNHL
                     EDKRKQCC"
BASE COUNT         3461 a         1842 c         2084 g         3363 t
ORIGIN      
        1 ctttgaagta attgctttac cggcaggttc tcctgctcca acggcagcta ctccgggagt
       61 attagcaact tcaaacccat ttgttgtaac tggtttagac ccaagtacag cgtatgattt
      121 ttatgtaaga gctttgtgta caggaccaaa cccatctagt ttatgggcag gaccggcaac
      181 agcgacaaca ttaccaacat gtccggcacc aattaactta atagtaattg gagcagacac
      241 caatacagca acaatatcat ggacaaacat tgcacctgct actctttttg aagtagttgt
      301 tcaacctgtt ggttcaggta ttccaaccgt tgcaggggtt attacttctc aaaacccata
      361 tacagccact gggttaagtg caggatttta tgagttttat gtaagagcaa tttgttcttc
      421 aacagattta agtgcatggg caggacctca aactttcttt atcttagctt cattgccagg
      481 atgtgcagga gttgatattg atttacaaac atcaacacca ggtgttctta acttatgtcc
      541 gggagaaagt tgtgtagatt tatctgctac tttctttgaa acgggagata ccacaactta
      601 taatgttaca ggaatcccat ttacgccacc attcccattt actggaggta ttccaacttc
      661 agtaaatatt gatgacagat ggtctaatgt aattgactta ccttttaact tctgtttctt
      721 tggacaaaat tataccaaag cattagttgg ttcaaatgga gtaatttcat ttgatataca
      781 gggtgaggtt cctggtggag cccaaatacc aaatggatat tgtgcttggc catttactca
      841 aactattcca aatgcagcct ttccaattaa agctgctatt tatgggccat atcaggatat
      901 tgatccgagt gtaacgacac ctccggctca accaagcatt aactaccaag ttttaggtac
      961 ggcaccttgt cgtgtgttag tagttaactt ctctgaagtg gcacaattta gttgtcaaaa
     1021 cagtgtgccg ttacaaacaa gtcaaattgt attatacgaa acgtctaacg ccgttgaggt
     1081 ttatgttcaa aaccgtgttc catgtttaac ttggaatggt ggtagagctg ttattggtat
     1141 tcaaaatcaa gcgggtactc aagcacattt cccaccggat agaaataccg gaacttggtc
     1201 tgcttctaac gaggcatgga gatttactcc ggcaggacca tcaaatgtga ctttctcttg
     1261 gttgaaagat ggagaatttt acagtaatga tttaagtatt aatgtttgtg tgtctgaaac
     1321 gacaagtatg acagctcaag ctatctatac tgcttgtgga ggagaacaaa ccattacaac
     1381 tgatacagtt ttacttaatg ttgttggtac tgaattaccg gtacaagacg atgttaccgt
     1441 ttgtgtaacg gaaggatatg aattacctac tttattggtt ggtaattatt ttacagagcc
     1501 aggcggacaa ggaactcaat taaatgccgg tgacattata actactgatc aaactattta
     1561 tgtttatcta gtaattaatg gaacaaatgg tgattgtaca agtgaaggaa gttttaatgt
     1621 aattacaaca actgaaattg tacctgaatt tgatcctatt ccaaatattt gtttgggagg
     1681 aacagctcct gcacttccgc caacttcctt aaatggaatt aacggtcttt ggacgccatc
     1741 gacgattgat acttctgttg aaggaacaat aaatctttta tttgaacctt tcggatctga
     1801 aggatgtgtg acaagttatc caactcaagt gacaattctt ccatttgatg atgtattacc
     1861 aattgaagat gttgttgctt gtaacagtta tattttaccc gatcttgctg ctggaaatta
     1921 ttatactgaa tcaggtggtt taggattaga agtgccatca ggaacagagg ttatagaatc
     1981 tacaacttta tatgtttata cagcaaatgg aatgtgtagt gatgaagaat catttacgat
     2041 tactattaat tctgtaacag gagatgtgct tccaaatgtt tcagcttgtg ataattatac
     2101 attaccaaca ttattggcca ataataatta tttcacagaa ccgggaggtt taggaacaca
     2161 attgaatgcg ggagatgtaa ttacaacaac gcaaaccatt tatatttata ctgaatctgg
     2221 aaccactcca aattgtgtgg ctgaaagtag ttttgttgtg acgattggtt ctctagtact
     2281 tgatccaatt gctgacgtag tcgcttgtga tagctatata ttaccagttt taacacaagg
     2341 taattatttc acagaaacag gaggaactgg aacacaatta aatgcaggtg acacgatagt
     2401 taccaatcaa ttaatttatg tttatgcagt tgatggaaca tgtctagatg agattagttt
     2461 cactgttaca atcaacacaa taagcgttga tgtaattgca gaccaaactg cttgtgatag
     2521 ttatacttta ccagccttaa gtgcaaataa ttcttattat acactagcag gaggtttagg
     2581 aacacaatta aatgcaggcg atgcaataac tacaacgcaa acgatattca tttatgctca
     2641 atcaggtact actcctaatt gtgtggctga aagtagtttt gttgtgacga ttggttcact
     2701 agtgcttgac ccaattgctg atgttgtagc ttgtgatagt tatatattac cggcattacc
     2761 tcaaggaaat tattttacag caacgggagg aacagggaca caattaaatg caggagatag
     2821 tatagagtca acacagttga tttatgtata tgctgctgat ggaacctgta cagatgagat
     2881 tagtttcaat gttacgatta acactgtaag tgttgatgta attgttgacc aaactgtttg
     2941 tgacagttat attttaccga ctctaagtgc aaataacact tactatactc aagcaggtgg
     3001 tttaggaaca caattaaatg tgggagattt gattacttca aatcaaacan nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ctgatgtaat accaaatgca acagaatgtt
     3181 tagcagacgg tggatttatt ttaccagcat taagtgcagg gaataattat tataccggtt
     3241 caggcggaac aggacagcaa ttagcggcag gtgatgtaat tacatcagat caaacaattt
     3301 acgtttttgc tcaaaccgca acaacaccaa attgtttcaa tgaaacaagc tttacggtaa
     3361 caattgttaa tgtaacggct caagagttat cagatgtaga agaatgtgga aaatatacat
     3421 taccggagtt aaattcaggg aatgcttatt acacaggaca aaacggtaca ggaactcaat
     3481 tacaaccagg atcagatgta actacgtcac aagtgattta tgtttacgct acagttggtg
     3541 gttgttctgc tcagacagaa tttgaagtaa ctatcaattc ttgtttgatt caaaagggta
     3601 tatcgccgaa taacgacggt agtaacgatt tctttgattt agttaacttc aatgtaagta
     3661 aacttgaaat ctttaaccgt tatggtacaa aagtatacag caaaaataat tatgaagacg
     3721 aatggtacgg tcaaagcgat aaaggtgacg aattaccgga cggaacatat tattatgtta
     3781 tatatttcac agaaggcact aatacgacag gatggattta tatcaataga gtacgttaat
     3841 tagaaaataa gtaagccggt tggctttaaa accaaccggc actactttaa ccctacaaaa
     3901 acaataaaaa tgaagaaaat atattttgca ctactgatag tattagtaag catagcagat
     3961 gtttcagccc agcaagatcc tcattataca caatacatgt ataacatgaa cgtgataaat
     4021 cccgcttatg caggtagtaa agagaacttg tcttttggct tactctacag aaaacaatgg
     4081 gtagagattg aagatgcccc tacaacgttt accctttccg gtagttctcc ggttggtaaa
     4141 aatgtaggtg ttggactttc attaatttcg gatgaaatag gtcctgtaac agaaaaaaat
     4201 gtatacggag atttctctta tacattaaat cttggtggag aacaccgttt agctttaggt
     4261 ttaaaagcag gggttacatt tcatgatgta gactttacta aaattatacc tactttgcct
     4321 gatccggcag atggtgtatt tgccaataac atcaataata catattttaa ttttggtacg
     4381 ggaattttct attatactga taaatattat ttagcatttt cagttcctaa tatgataaat
     4441 tctaaacact tagattatga tgggagagaa tatggtagtg aaaccacaca ctatttcttt
     4501 accggaggtt atgtgtttga tttgtctgcc aacgtgaagt ttaaaccatt ctttatggtt
     4561 aaatcagcat ttagtgctcc tacttcgtta gacctttcca caaatttctt atttaatgag
     4621 aagtttgaaa ttggagcaac ctatcgatta gaagacagtt tcggtgccat ggttaattat
     4681 gctatttctc caagtttgag aattgggtat gcttacgacc atatcgtttc tgatttgaat
     4741 ataacaacgc ctgcctctca tgagattatc ttgttgtttg atttgaattt cccgaaaaag
     4801 gtatcacgtt catcacgatt tttctaacct aataagcaaa ccagacgatg aaaaaagtat
     4861 atatattttt acttagtatt gtaatgttaa gcattactcc actgagtgca caaaacaaag
     4921 acactaaaaa agcagataag ctttttaagc aatttgatta ttttgaagcg gcagatgaat
     4981 acctgaaatt agctgaaaat ggcaaaggtg acggatacgt tcataaacaa ttagcggata
     5041 gttactataa tatgtttaat gctgctgaat ccagtaaatg gtatgctaga gctgtagaaa
     5101 cgccacaaga tgccgaaaca tattatcgtt atgctcaaat gcttaaagca caagggaaat
     5161 acgaagaagc taacaaacaa atgcaacagt ttgcaactaa agctcctaat gaccaaagag
     5221 caaaagattt taaagctaat ccaaactatt taccaaaatt gttagataaa cagaaacaat
     5281 ttgatgtgaa ggctttagaa ttgaacagtg acaagtctga ttttggagcg gtattgcacg
     5341 aaaactcatt gtattttgcc agtgcaagaa acggagctaa aaaaacgtat ggttggactg
     5401 acgagcctta tttggacata tacaaagcaa actacaatgt tgacggaaca atcaccaatg
     5461 ctgagccggt tgcggaagta aattcaaaat ggcatgatgg tccagtaacg ttgagtgctg
     5521 atggtaatac aatgtatttt gccagtgaga gtttcaaaga aaaaagtttt gaaaaagata
     5581 aagtgaacaa tactcgattg aaagtgagtc aagtgaacct tttcaaagca acgaatgtta
     5641 atggaaaatg gagtaatatt accattttac ctttctgcag taatgcctat tctaccggaa
     5701 atccttattt gagtaaggat ggaaaaacat tatacttctc ttcaaatatg ccaggttctt
     5761 taggtggaaa cgatgtttgg aaagtagcag taaatcaaga tggaggtttt ggtacacctg
     5821 agaatttagg agctaaaatt aatactgaag gagatgaaag tttcccttat atatctgatg
     5881 acaacaaaat gttctatttc gcttcaagtg gaagacaagg tttcggtgga tatgatgtct
     5941 ttgtttatga tatggcaaaa ggggaatctg ttaatatggg taaaccggta aacagtgaaa
     6001 aagatgattt tgcttttacg ttcagtaaag ataaaaatat tggattcttg tctagtaata
     6061 gagccggagt tgataacatc tatttagcta caccggtatg tggtgttgaa gttactacca
     6121 ttgttactga tgctaaaaca ggagccattt tagtgaatgc caaagttgcc attgtggacg
     6181 ataagaaaaa tgttatcgca actgaaatga gtaatgcgaa aggtgaagtt acttattatg
     6241 ttgaatgtga taaaaattat actgttcagg catctaaaga cggttacgaa agtaatactt
     6301 tcccggttgc agcgagcaaa gggccacaaa agcgagtgga tgcaccactt aacccaattg
     6361 acgtgattgt aacagaaaca gaagtgattt taaatccgat cttttttgaa tttgataaaa
     6421 gtaatatcac gcaagaagga gcctttgaat tggataagtt aatacaagta atgcaaaata
     6481 atccagagat ggttatcatg gctaaatcac acacagacaa tcgtggtagt gatgtgtaca
     6541 acttaagttt atccgacaga agagcaaaat ctacggttca atatattatt tctaaaggaa
     6601 tttctaaaga tagaatctcc ggtaaaggaa tgggtgaaac agaacctaaa gcggattgta
     6661 aagaagcttg tacagaagaa caacatgctt caaacagacg tagtgaattc ttaattgtaa
     6721 agaaataaac acaacaagaa tttaaatata ctaaacgggt tttcgaaaga aagcccgttt
     6781 ttgttttacc atggaattga acttgttctt gagctaaaat aatttgccgg tcctgaattg
     6841 cctccaaaat tatttccgga actaaggcga taaaaaggag cattacccca aaaggcaggt
     6901 ttgtcttcta tcagcatatc ttccccagtg tcattagaaa tatagggacc aattttgaat
     6961 tgccatgaag ctattctatt tctgcttttg gtgggatcaa aatagtcgtt gttgctgtca
     7021 gccaattctt taatgtaagt tttgaatttt gtttgatatt ccggaatggc caatattttc
     7081 gttatgagtg gtctaccact tgccggaccc caattcaaag ggttttgagt tcctgagtta
     7141 ggcatcaaca tagatgttcc caatgtattg tcgtaatcat aaggaataaa atagactttt
     7201 ccgtcaggag ccatgtagaa ataaaagtta ttggcattaa cccaataatc atcccacatg
     7261 cctaccataa tgttgacggc atatgttttt aaaaataagt caacatccat ttgttggcta
     7321 atccaggttt taaatgctga acctgtttta gaatttaagt tattgataaa ggagataagt
     7381 tgtgttttgg ctccgtcaag ttcattttct ctcgttttaa gatcataagc ataataaaga
     7441 gagtttgacg gatttaaatc tacctcttca actccgatgc tttcggtttg cacaaaatcg
     7501 gcattttgat accctgccca acctcctttc cacataaatc ctatggattg tgtccaattt
     7561 tcctttcggt aattgataaa atcctcatca acgctttcca gcattacata tacgccgtaa
     7621 taggcaggag caacatcacc ttccactttt atggaaacac ggcaatagga agctcttgga
     7681 gctgtccaaa tgccatatcg acgaaataag tcgtagcaat atatttcacg tacataagtg
     7741 gcatcgtctt taaaccattt caagttcagt ttttttagtc cttgaaagcg ttgggttttt
     7801 atgtttttgt tgaaatcaac tccgaaatga cagtggtgcc aatcagggtt tgaagaagta
     7861 tggagttgcc cgattgcacc ttcaggtcga cgacggctgg tgttcccgcg tagttttaag
     7921 ccaatactgt cgagttcaat ggtgttgcca tccaagctgt agcgatactt tgccacaact
     7981 ttagtttcat tttttgggtt caggtcgtag ttagtcagca ataaattcca ttgctcttca
     8041 gtcagttcta atgtaatttc aggaacttta tctaagttaa atacatcggt tggcgtagat
     8101 ggatttattg gtattgtaat atcatcctca gtattgcatc ggataaaaaa caaacttcca
     8161 aagagtaatg caattaaaaa gaatatcctt gtgcttttca taactaaaga tttacacaaa
     8221 ggtagtaaat gatgatatgt aaagttaact aggacgtttg ttctttggtg taatattttt
     8281 ttctaaacca aaaagcgagg tttactaagg ctattaaagc cggtacttct accaatggac
     8341 caattactcc ggcaaaggct tgaccactgt tgataccaaa aacgccgatg gccactgcaa
     8401 ttgctaattc aaaattattt ccggtagcag taaaagctat tgctgttgat ttggaataat
     8461 ctgcaccaaa atattttcca acgaagaaac taaggacaaa catgatcgtg aaataaataa
     8521 ctaatgggat agcaatacga acgacatcta atggaatttg aacgatcagt tctcctttca
     8581 aactaaacat gagtacaatt gtaaacaata aagcgattaa tgttattggt gaaataaagg
     8641 gcacatactt attgttaaac cattcagttc cttttaaggc aatcaaagaa taccgactga
     8701 taacccctag agcaaaagga atccccagat agatgccgac actttcagct atttgtccaa
     8761 tactaatgtt aacttcaaaa ctatcgtaac caaaataagg aggcaaaatg gtgatgaaaa
     8821 tatatgcata aacactatat agcactactt gaaaaatact gtttaaagca attaagccgg
     8881 ctgcatattc ccgattccca tcggctaaat cattccatac aacaaccatg gcaatacaac
     8941 gagctaatcc aatcagtata acaccaatca tatattccgg atagccatta agaaaaagta
     9001 aggccaataa aaacattaaa atagggccta ctatccaatt aagaaacaac gatgcaccca
     9061 aaacttttgt gtttttaaac acttctccca tttgttcata tttgactttg gcaagaggtg
     9121 gatacatcat caggattaat ccaactgcta atggaatgtt agtagttcca ctggaaaagg
     9181 agttgataaa tccactactg gagggtataa aatagccaat agaaacacct atcgccattg
     9241 cgaggaatat ccaaagagtt aaaaagcgat ctagaaagcc cagttttttt cgttccacaa
     9301 cgggggcaca attgtttgat gacatagctt atttttttat ctgtgcaaaa acataactca
     9361 tctctgtggc tatttgtaag ctgcgttctt catattttat cgcttgttgt ggggtgttgt
     9421 caaaagcttt agggtcttca aatgtaatag ggatacgttt ttccgcaccg gcaatgaacg
     9481 gacagccacc atcggcttgt gaacaggtca tgatggcagc aaattcactt tgcggattga
     9541 aagcatcgtc ataggtttta gaaaaaccaa ggataggatg tgcattttct gcaaatttaa
     9601 tagcataaat aggattggtt ccttcagcga ttgtttggat ttgaaagcct gcattttcta
     9661 atgtttttgc tgccatagga aacatagcgg ttgcttcagt tccacccgaa tagcaatata
     9721 cattagggat ttcgtaatac gttgctgcgg cttgtgccca tacttgtgct aaatgacttc
     9781 ggcgggaatt gtgggtgcaa attaaattta atcggacgtc ctgtttattc gttactttga
     9841 tttgaatgta atcaattaaa gattgtaggg tttgttttct atcagcagtt atcgaatcaa
     9901 aagaaatctc agaaaggtat ttttctattt taggatatag tttcatggat taaaagatat
     9961 aattataact tgttttcaat ttctaacaac aactgccgcc cggggtgcaa caacttggtt
    10021 cattattcat atcagacaaa cgcacttttg gtttttgtgt aggaacacca cactgatctt
    10081 gagccaaaca cgcggttttc ttgttaacta aaacaaacga ttggccgtta aagtctaaat
    10141 cgtatttgcc gatggtttct ccttggtatt ccacttcaat ttcatggtct tcaatgccta
    10201 acactctttc agacaaggcg ataatattgg ccaatttttg tggttttaac cgatggtcat
    10261 aatcgttggc atcccataat tggaagttaa caacggtttc tttgcgaacg gttccgccgc
    10321 aatcaatgaa gtttttggtg attaaaccca cttcggttac atgaaaatgt tccggtgcaa
    10381 atgttccgtt tggtaattga aaagtaacgt tttctactgt agaaagtacg tttttgattt
    10441 gtgatagttt catattttag gtgctaagtt tatttcggtt ctactctttt taaaattgat
    10501 tttggtttaa caacattgtt ttcttttatc ttccaagtgg ttgtttaccg tttcaaaaaa
    10561 ggtttttatt ttatcaaaac ctgtttcatt gaggcaatag caaatggaaa ccccttccac
    10621 gtttccttta attaaacctg catttttgag ttctttgagg tgttgggaaa ccgttggctg
    10681 agctaagggt aattcattta cgatatcacc acaaatacag gaatcaacct ttagtagata
    10741 ttcaataatg gctattctgg ccggatgtcc gattgctttt gctaaaatag caagttcatt
    10801 ttgttggtcg gtaaagtaat ctgatttagt aattcccatg cttatatatt
//
LOCUS       DAOTCW010000073        10847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3920_length_10847_cov_45.501761, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000073 DAOTCW010000000
VERSION     DAOTCW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10847
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3920_length_10847_cov_45.501761"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="P5335_07220"
     CDS             <1..459
                     /locus_tag="P5335_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HRZ74703.1"
                     /translation="DVFLLETGTVHAIGGGILLAEIQQTSDITYRVYDWDRVDANGNG
                     RELHTELALDAITYQTTSAKKEYTAIENKSISVVDCPYFKTNIIELKGEIPIHKSKDA
                     FTVLMCTDGNFELCLNDEKYVYKAGDTVLIPAVLTSFLLKGKATLLEITV"
     gene            530..796
                     /locus_tag="P5335_07225"
     CDS             530..796
                     /locus_tag="P5335_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74704.1"
                     /translation="MASIKNLKKDINFVMGDIIEAVYIHEMTTTGKPTEKSNALIDEA
                     IATFDTLISKVNDKKVESRKAHLKQVEKDLESAAIQLVEKVNAL"
     gene            876..949
                     /locus_tag="P5335_07230"
     tRNA            876..949
                     /locus_tag="P5335_07230"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:910..912,aa:Thr,seq:tgt)
     gene            complement(1092..1820)
                     /locus_tag="P5335_07235"
     CDS             complement(1092..1820)
                     /locus_tag="P5335_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HRZ74705.1"
                     /translation="MKFITKIALLFTILLLFAACEKENKKDYNLHISGNIKGIKQGKL
                     FIQQLKDTSLVVIDSIIFNGKSSFDSYLTIDSPEMLYFFLDRGQTNSLDNNLVVFAEP
                     GELSLETNLETFYADAVVKGSKNNDLYLNFKKINARFSNQQLGLIEKEINAQKNSNLV
                     QLDSIQKAKEQLLKRKYLYAINFALTQKEYEVSPYIALSEIYDAQIKYLDTIHHSMSP
                     KVAQSKYGKLLTEFIEKRKKEEQQ"
     gene            complement(1963..3822)
                     /locus_tag="P5335_07240"
     CDS             complement(1963..3822)
                     /locus_tag="P5335_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HRZ74706.1"
                     /translation="MKKSFLSIVFTGTILLLNLSIATAQNKTNEVQVSLDLVTINNDR
                     VAVTIIPPKIKTSETTFYLPKIVPGTYSEDDYGRYVDNFKAYDAKGKELVIAKMDENS
                     WVIKEAKSLAKVTYLVNDTYDSESGSEFGKGDIFSPAGTNIAEGLNFVINTHAFVGYF
                     KDLKELPYRLSIIKPTNLFGATAMIDLDKSESSDVFTASRYAELVDHPIMYNKPNYTT
                     FMVDDMEIILSVYSPKGTFKADDLKPTMEKMMKAQKKFLGKVNSTKKYAILLYLSDYE
                     TSDAKGYGALEHNTSTTVVFPEAMPAEQLEQSLVDVVSHEFFHIVTPLSVHSKEIHYF
                     DFNTPKMSQHLWMYEGITEYFANLFQVNQGLISEDDFYERMAGKITNSKRYDETMPFT
                     KMSAEVLKKQYKDAYLNVYEKGALIGMCLDIQLRESSNGERGILDLMQKLSKEFGDAK
                     PFDDSELFDKIISLTYPEIRTFLTNYVAGSTPLPYDEFFGKMGVTQTKIQIPGNVFLK
                     GQTPYITVNPSTKEIIVLPGIELNSFMKSLGLQGGDIILAINDTAYNLDNIYDMIIGS
                     MSWQENDPITFKIKREDKEITLKGTVQIPMDEVDGYQATDDAKKTIREAWLKG"
     gene            complement(3932..5329)
                     /locus_tag="P5335_07245"
     CDS             complement(3932..5329)
                     /locus_tag="P5335_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRZ74707.1"
                     /translation="MKKISLLVVLFYSIASFSQDITGEWYGKISVMGSELRLSFTISK
                     NENVYSGTMSVPQQNANGIPLSSVTFQDNLLKITFDAAGFSYNGKLNAETVFEGTFSQ
                     NGQNFPLNLSREKMEETKLNRPQEPKGKFDYGIEEVTFENKKDKITLAGTLTYPQINK
                     PYPVVVLISGSGPQDRNSEIFGHKSFWVIADYLTKNGIGVLRFDDRGFAKSQGDRSLA
                     TSYDFATDVEAAVAFLKTKSGVDSKKIGLIGHSEGGMIAPIVASKDKTIDFIILLAGP
                     GIPCMELLLDQAYQIGKVAGMTEEQLSETRKINQSIYTIVKSDADDETVKKELTTILE
                     KITNENSQLEQLSESDKKQMIAQQIEQLSSPWYRSFMKFNPSIYLEKVKCPVLALNGE
                     KDLQVPSKANTEGIKNSLSKGGNKKVTLKVYPNLNHLFQECETGAVEEYGTIEQTFSP
                     TVLTDIKDWILLQTK"
     gene            complement(5341..5919)
                     /locus_tag="P5335_07250"
     CDS             complement(5341..5919)
                     /locus_tag="P5335_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74708.1"
                     /translation="MKQLYNPFELYSERQLLIFGLVLTLIGSFLGYLFNGRFDGIIDF
                     HLLESVTFFEVVLDNFFNTVLLTTLLFITGKLINSKTRFVDVLNASLLARIPFYILPF
                     FNFNNVMYDVSNRTYSIIVAGSFNAISTPDMILLMLFSFVAICTLIWFSVLLWNGFKV
                     ATNAKGIKNIILFIFTILVAEVVSKYLITTFN"
     gene            complement(5931..6410)
                     /locus_tag="P5335_07255"
     CDS             complement(5931..6410)
                     /locus_tag="P5335_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74709.1"
                     /translation="MNEKPLFQKNQKFTQWWVWAIIIGANLIGGYSLLNEFENNKLSL
                     LFLNLYFLFFGIFILFFYFLKLETIITKDYISIRFFPFHWKERKYFFSEINQMEVIKY
                     SPIFDYGGWGVRYGRNGKAFNVAGNMGLELHFLNRKNLLIGTQKPEELKKVIETIIK"
     gene            complement(6415..6690)
                     /locus_tag="P5335_07260"
     CDS             complement(6415..6690)
                     /locus_tag="P5335_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HRZ74710.1"
                     /translation="MKPKLPIHCPSCESSLSVSQLTCETCQTQVSGNYSLPLLLKLSE
                     DEQNFILQFFLSSGSLKEMAIQMGNSYPTVRNKLDDMIEKIKKTQNK"
     gene            complement(6819..7106)
                     /locus_tag="P5335_07265"
     CDS             complement(6819..7106)
                     /locus_tag="P5335_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HRZ74711.1"
                     /translation="MSLLLKDFTAVQIDRIIEMAWEDRTPFEAITFQFGISEQQTIEL
                     MRTEMKVSSFKMWRERVQGRATKHAKKRVNGIDRFKCTLQRQITHNKISKR"
     gene            complement(7174..7446)
                     /locus_tag="P5335_07270"
     CDS             complement(7174..7446)
                     /locus_tag="P5335_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HRZ74712.1"
                     /translation="MKKSKRVELDNEQIERVISMAQEEKKPFEVIKVEFGISENEVTE
                     IMRKRLSKDNFELWKKKVTASKPKPKPIIDDFDDLDSKYYIKNKFD"
     gene            complement(7549..8373)
                     /locus_tag="P5335_07275"
     CDS             complement(7549..8373)
                     /locus_tag="P5335_07275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRZ74713.1"
                     /translation="MQTIIDYFESISSLHRGLILVGGITLFWLIENAYPLFKFQYNKI
                     EHAGINIFFTMTTIIINFMMAFILLLAADYAIQQNFGILQWLPQMNIWIYTIIGLLLL
                     DFIGAYLVHLIEHKVTFLWRFHLIHHTDTWIDTTSANRHHPGESIIRFIFTVIGVLIV
                     GSPMWMVFLYQSLSVIFSQFNHANIPLPKKMDKLLSYFIISPDMHKVHHHYKLPYTDS
                     NYGNIFSVWDRLFGTYSYLDREKIIYGVDTHMKPEENNQLVNLLKIPFQKYRAPKL"
     gene            complement(8421..9023)
                     /locus_tag="P5335_07280"
     CDS             complement(8421..9023)
                     /locus_tag="P5335_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HRZ74714.1"
                     /translation="MNSLFPKEKIVFDLPDAEIEYYPNLFSVEKSNELFQKLKNEIPW
                     QQDNITVFGQTYPQPRLTALYGNDGKTYSYSNIVMQPHKWNPLLMFIKNEVEEICPEN
                     FTTVLLNQYRDGKDSNGWHADNEKELGRNPIIASISFGAERVFHLQHNEIKECKQKIT
                     LEHGSLLIMKGETQHFWKHQIPKTSKPIGTRINLTFRIIR"
     gene            complement(9088..>9378)
                     /locus_tag="P5335_07285"
     CDS             complement(9088..>9378)
                     /locus_tag="P5335_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRZ74715.1"
                     /translation="AEFVLTMFFLLIILGATDKFANGKFAGIAIGLGLTLIHLISIPI
                     TNTSVNPARSTSQAIFVGGEPLSQLWLFWLAPILGAIVAGFIYKNVLQKNEE"
     assembly_gap    9379..9478
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9588..10484)
                     /locus_tag="P5335_07290"
     CDS             complement(9588..10484)
                     /locus_tag="P5335_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRZ74716.1"
                     /translation="MFGNRVLKGVFLVALGATSYGMLATFVKLAYHENFTTAEVTTAQ
                     FVYGIIGVLLINSFQRVKNKNIAFKATGKNIFHLMLAGTSLGMTSVFYYLSVKYIDVS
                     IAIVLLMQTVWMGVLLEMILDKKLPSIQKIMAVVIVLIGTVLATNLIQNEIAIDWRGL
                     FWGILAAASFTTTMFTANRVATEITSAQRSLYMLLGGAIIVFLFSIVTQSTPFNFEIF
                     LKWGIIVALFGTIIPPMLLNAGFPLTGIGLGSIVSSLELPVSVLMAYFLLSEKVNGLQ
                     WLGIILIILAIVVMNIKFIRKI"
     gene            10610..>10847
                     /locus_tag="P5335_07295"
     CDS             10610..>10847
                     /locus_tag="P5335_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HRZ74717.1"
                     /translation="MQKLSLIILLSFVCCQNTIHEVPINYNSFHQEALTFCKQNKFNQ
                     DFYFFVDLSIHSGKNRFFIYDFKNQKVVNQNLVTH"
BASE COUNT         3599 a         1634 c         1665 g         3849 t
ORIGIN      
        1 gatgtttttt tattagaaac cggaactgtt catgctattg gtggtggaat tttattagcg
       61 gaaattcaac aaaccagtga tattacttac cgagtttacg attgggacag ggtagatgct
      121 aacggaaacg gacgtgaatt gcacacggag ttggctttag atgccatcac ttatcaaacc
      181 acatctgcaa aaaaagaata cactgcaatt gaaaataaat ccatttcagt tgttgattgt
      241 ccttatttta aaacaaatat tattgaactc aagggagaaa ttccaattca taaaagcaaa
      301 gatgcattta ctgtattgat gtgtactgat ggaaattttg agttatgttt aaatgatgaa
      361 aagtacgttt acaaagcagg tgataccgtt ttaataccgg ctgttctaac atcattttta
      421 ttaaaaggaa aagcaacatt attagaaatt actgtttagg aaacgaaact aatttattta
      481 attctaattt tttacgtact tttgcaccgc aattaaaata tatataaaaa tggcaagtat
      541 taaaaatttg aagaaagaca tcaattttgt gatgggagat attatcgagg cggtttatat
      601 ccacgaaatg acaactactg gaaaaccaac tgagaaatca aatgcgttga ttgatgaagc
      661 tattgctact tttgacacgt taatttctaa agtaaacgac aaaaaagttg aaagtcgtaa
      721 agctcattta aaacaagtag aaaaagattt ggaatcagct gcgattcaat tggttgaaaa
      781 agtgaatgca ctttaatccg attttttgat aaaaaaagtg cctttttgtt ttggaatatt
      841 caatttcaaa attatatttg cactcagatt gagacgccga tgtagctcag ctggctagag
      901 cagctgattt gtaatcagca ggtcgtgggt tcgagtccct ctatcggctc tattttaagg
      961 acaactttta aggttgtcct ttttttttga aatactgtga acctaaagat aagttaattt
     1021 gatagtatgg aaattgcttt aattgtattt attaaaaaaa accataagtt ttaatcttat
     1081 ggtttttagt tttattgttg ttcttccttt tttcgttttt caataaattc ggtcagtaat
     1141 tttccatatt tagactgagc aactttgggc gacattgaat gatgaatggt gtctaaatat
     1201 ttaatttggg catcataaat ctcagacaaa gcaatatatg gactcacttc atattctttt
     1261 tgagttaaag caaagtttat agcatataaa tatttgcgtt taagtaattg ttcttttgct
     1321 ttttgaatac tgtctagttg cacaagattg ctgttttttt gagcatttat ttctttttct
     1381 atcaacccta attgttgatt tgaaaaacga gcatttattt ttttaaaatt caaatagagg
     1441 tcattgtttt ttgaaccttt tacaacagca tctgcataaa aagtttctaa attagtttct
     1501 aatgaaagtt ctccgggttc agcaaaaact actaaattat tgtctaagga gtttgtttgg
     1561 cctctgtcta aaaagaaata taacatttcc ggtgaatcaa tggttaaata gctgtcgaaa
     1621 gatgattttc cattgaaaat tatagaatca atcacaacta aactggtgtc ttttagttgt
     1681 tggatgaata attttccttg cttaattcct tttatgtttc ctgaaatatg caggttatag
     1741 tcttttttat tttctttctc acaggctgca aataaaagaa gtatggtaaa aagtagggca
     1801 attttagtaa tgaatttcat ttgtttttat gtcttttgaa tttggttgca aaatagggat
     1861 tttttgaaaa acggaagttc ttttttaggg gatagttttt aaaaaataaa aatccctgaa
     1921 tgattacttt cagggatttt tatcttttta atatattttg atttaacctt ttagccaagc
     1981 ttcacgaatt gtttttttgg catcgtctgt agcttgatat ccatctactt catccattgg
     2041 aatttgtact gttcctttta aggtaatttc tttatcttct ctttttattt taaaagtaat
     2101 tggatcattt tcttgccatg acatgcttcc aataatcata tcataaatgt tgtctaaatt
     2161 atatgctgta tcattaatgg caagaataat atctccgcct tgtaatccta gtgatttcat
     2221 aaaactattt aactcaattc ccggtagaac aataatttct ttggttgaag gatttacggt
     2281 gatatagggt gtttgtcctt ttagaaaaac atttcccgga atttggattt tagtttgggt
     2341 aacacccatt ttgccgaaaa attcatcata aggaagcggt gtagaacctg caacataatt
     2401 agttaaaaaa gttcgaatct caggataagt cagagaaata attttatcaa ataattcgct
     2461 atcatcaaaa ggtttagcat caccaaattc tttagataat ttttgcatca aatcaagaat
     2521 tcctctttcc ccatttgagc tctcccgtaa ttgaatatcc aaacacattc caattaaagc
     2581 tcctttttca taaacattta agtaagcatc tttatattgt ttttttagaa cctctgcact
     2641 cattttagtg aatggcatgg tttcatcata gcgtttagaa ttagtaattt taccagccat
     2701 tctttcataa aaatcatctt ctgaaattag tccttggttc acttgaaata aattggcaaa
     2761 atattctgta attccttcat acatccataa atgctgtgac atctttggag tgttgaaatc
     2821 aaaataatga atctctttgg aatgcacact taaaggcgta acaatatgaa aaaattcatg
     2881 cgaaaccaca tctactaaac tttgttctaa ttgttcagcc ggcattgctt ccggaaaaac
     2941 aacagtggtt gaggtattgt gttctaaagc accatatcct ttagcatctg atgtttcata
     3001 atctgataaa taaagtaaaa tggcatattt ttttgtgctg tttacctttc ctaaaaattt
     3061 cttttgggct ttcatcattt tttccatcgt aggtttcaaa tcgtccgctt tgaatgttcc
     3121 tttaggagaa taaacactta aaataatttc catatcatcc accataaatg tggtgtaatt
     3181 tggtttatta tacataattg gatgatctac caattcagca tatcttgatg ctgtaaatac
     3241 atcactactt tcactcttat ccaaatcaat cattgcagta gctccaaata aattagtagg
     3301 ttttatgata gataatcgat atggcagttc tttcaagtct ttgaaataac ctacgaaagc
     3361 atgcgtgttg ataacaaagt tgagtccttc ggcaatgtta gttcctgcag gtgaaaaaat
     3421 atcccctttt ccaaactcag aacctgattc agaatcatag gtgtcattta ctaaataagt
     3481 aacttttgct aacgatttag cttcttttat tacccatgaa ttttcatcca ttttagctat
     3541 cacaagttct tttcctttag catcataggc tttgaaattg tcaacataac gtccgtaatc
     3601 atcttcggag taggttccgg gaactatttt cggaaggtaa aaagtggttt cacttgtttt
     3661 tatttttggt ggaattattg ttacagccac tctgtcatta ttaatagtga ctaaatctaa
     3721 agaaacctga acttcgttgg ttttgttctg agcggttgca atggaaaggt ttaacagtaa
     3781 aattgtaccg gtaaagacta tcgataaaaa tgattttttc atgaaataat tatataattg
     3841 ataataagtt gcaatagttc ttattttgtt acaaataaca acaaaaaacg cttttattca
     3901 aagcgtttaa ctgttaagtg tttgataaag tttatttagt ttgtaataga atccaatctt
     3961 ttatatctgt taatacagta ggagaaaagg tttgttcaat tgttccgtat tcttcaactg
     4021 caccggtttc acattcttga aataagtgat ttaaattagg gtatacttta agggtaactt
     4081 ttttatttcc accttttgat aacgaatttt taatgccttc tgtatttgct ttagagggaa
     4141 cttgtaaatc cttttctcca tttaaagcca gaacaggaca ttttactttt tctaaataga
     4201 tgcttggatt aaatttcata aaacttcgat accaaggaga gcttaattgt tcgatttgtt
     4261 gggcaatcat ttgcttttta tcagattcgc taagttgttc taattgggag ttttcatttg
     4321 ttattttttc cagaatggtg gttaattcct ttttaaccgt ttcatcatca gcatcacttt
     4381 ttacgattgt gtaaatagat tgatttattt ttctggtttc acttagttgt tcttccgtca
     4441 ttccggcaac ttttccaatt tgataggctt gatccaataa aagttccata cacggaattc
     4501 cgggaccggc taacaatatg ataaaatcaa tagttttatc ttttgaagca acaatagggg
     4561 caatcatacc accttcgcta tgtcctatta atccaatttt tttagaatca actcctgatt
     4621 ttgtttttaa aaatgcaact gcagcttcaa catctgtagc aaaatcataa gaagtggcaa
     4681 gtgaacgatc accctgagat tttgcgaagc ctctgtcgtc aaagcgaagc actcctatgc
     4741 catttttagt taagtaatcg gcaatcaccc aaaaagattt atgtccaaaa atttctgaat
     4801 tacggtcttg cggtccactt cctgaaatca ataccactac cggataaggt ttattgattt
     4861 gtggatatgt taaggttcct gctaaagtta ttttgtcttt tttattttca aaagttactt
     4921 cttcaattcc ataatcaaat ttaccttttg gttcttgtgg tcgatttagt ttggtttctt
     4981 ccattttctc ccgtgacaga tttaatggaa agttttggcc attttgagaa aaagtacctt
     5041 caaaaacagt ttcagcattt aattttccgt tataagaaaa tccggctgcg tcaaacgtaa
     5101 tttttaaaag attatcttga aaagtaacgg atgataacgg aatgccattc gcattttgtt
     5161 gcggaacact cattgttccg gaatagacgt tttcgttttt ggaaattgta aaacttagtc
     5221 ttaattctga tcccatgaca cttatttttc cataccattc accggtaata tcttgagaaa
     5281 atgatgctat tgaataaaaa aggacaacta ataaactgat ttttttcatt tttataaaga
     5341 ttaattaaaa gtggtaatta aatattttga aacaacttct gctacaagaa tggtaaaaat
     5401 aaatagtata atgtttttaa ttcctttagc atttgtcgct actttaaaac cattccataa
     5461 aagcacggaa aaccaaatca aagtacatat tgctacaaag ctgaatagca ttaacagtat
     5521 catgtccgga gttgaaatag cattaaaact accagcgacg atgatgctat aagtacgatt
     5581 actcacgtca tacattacat tgttgaaatt aaaaaaaggt aatatataaa aaggaattcg
     5641 tgcaagtaaa gaagcgttta atacatcaac aaaacgcgtt ttcgaattga ttaatttccc
     5701 ggttataaac aatagcgtag ttaataggac agtattaaaa aaattatcga gaactacttc
     5761 aaaaaaagtg actgattcta ataaatgaaa atcaattata ccgtcaaagc gaccattgaa
     5821 aaggtagcct aaaaaggaac caattaatgt caatactaat ccaaaaatta aaagttgacg
     5881 ttctgagtaa agttcaaaag gattatataa ttgtttcatt atttagtcaa ttactttata
     5941 attgtttcta ttactttttt taattcttca ggtttttggg tgccaattaa aagatttttt
     6001 ctgtttaaaa aatgcaattc taatcccata tttccggcaa cattaaatgc ctttccgttt
     6061 ctgccgtatc gaacacccca acctccataa tcaaaaattg gactatattt gattacttcc
     6121 atttgattta tttctgaaaa aaaatatttt ctttctttcc aatgaaaagg gaagaaacgt
     6181 attgatatat agtcttttgt gatgattgtt tcaagtttta aaaaataaaa aaataagata
     6241 aaaatcccaa aaaataaaaa atagagatta agaaataaaa gagatagttt gttattttca
     6301 aattcattta acaatgagta tcctcctatt agattggcac caataatgat tgcccaaacc
     6361 caccattgtg tgaatttttg atttttttga aataatggtt tctcattcat tggcttattt
     6421 attttgcgtt tttttaattt tttctatcat gtcatccagt ttatttcgaa cagttggata
     6481 actattgccc atttgaatgg ccatttcttt taaacttcca ctagaaagaa aaaactgcag
     6541 tataaaattt tgttcatctt ctgaaagttt caatagcaat ggtaaagaat agtttccgga
     6601 aacttgtgtt tgacaagttt cacaagttaa ttgtgaaaca gagagagagc tttcacaact
     6661 cggacagtga atgggtaatt taggcttcat tttaaataaa gtttaaacaa aagtaaataa
     6721 tatttaaata aatattaata taatttatat ttattttatt ttttccaata aaaaaagcat
     6781 tctgagagaa gaatgctttg tgtttattga atatgttttt atcgtttgga tattttgttg
     6841 tgtgtgattt gacgttggag tgtacatttg aagcggtcaa tgccatttac tcttttctta
     6901 gcatgtttgg tagctcttcc ttgtacacgt tcccgccaca ttttaaaact cgaaaccttc
     6961 atttctgttc gcatgagttc aatggtttgc tgttcagaaa ttccaaactg aaaagtgatg
     7021 gcttcaaaag gggtgcgatc ttcccaagcc atttctatta ttcggtctat ttgcaccgct
     7081 gtaaaatctt ttagaagtag agacattttt aatcaaaaag caaatatcgt ctgaaaataa
     7141 aaaaactctt ggtgaacaag agttaaaata tacttaatca aatttatttt tgatatagta
     7201 tttgctgtct aaatcatcaa aatcatcaat aattggtttt ggtttaggtt tactagccgt
     7261 aactttcttt ttccaaagtt caaaattatc ctttgaaagt cgttttctca tgatttcagt
     7321 aacttcattt tctgaaattc caaattcaac cttaattact tcgaaaggtt ttttttcctc
     7381 ttgagccata ctgataacgc gttcaatttg ttcgttatcc agttccactc ttttactttt
     7441 tttcattagt tcaaaagcac aattaatatt taatgtaaag tttcaaattc taaaccaata
     7501 ttagtaaaaa aataaattac tttttcaaac tacacttatt tttttaaatt aaagtttagg
     7561 agctctgtat ttttgaaatg gtatttttaa caaattgact agttggttgt tttcttccgg
     7621 tttcatatgg gtgtcaacgc cataaataat tttttctcta tctaaataag aatacgttcc
     7681 aaatagtcta tcccaaacag aaaatatatt tccgtaatta ctgtctgtgt agggcaattt
     7741 atagtgatgg tgcactttgt gcatatcggg cgaaataata aaataactta acaatttatc
     7801 catttttttg ggaagtggaa tattggcatg attaaattgc gaaaaaatca ctgagagtga
     7861 ttggtataaa aaaaccatcc acatcgggct tcctactatt aaaacaccaa taaccgtaaa
     7921 gataaaacga atgatgcttt caccgggatg atgtctgttg gccgaagtag tgtcaatcca
     7981 agtatcagtg tggtgaatta aatgaaaacg ccataaaaaa gttactttat gttcaatcaa
     8041 atgaactaga taggctccaa taaaatcgag taaaagtaat ccgataatgg tgtaaatcca
     8101 tatattcatt tgcggtagcc attgtagaat tccaaaattt tgttgtatag cgtaatcggc
     8161 tgctaataac aaaataaaag ccatcataaa attgatgatt atagtggtca tcgtaaagaa
     8221 aatgtttatt cctgcatgtt caattttatt gtattgaaat ttgaataatg gataagcgtt
     8281 ttcaattagc caaaaaaggg taatgcctcc aaccagaata agacctctgt gaagtgagga
     8341 aatgctttca aaataatcaa tgatagtttg catttatttt ttttactaat ttaaataata
     8401 ttttgattta attttagcga ttatcttata attctaaaag ttagattaat tctagtgcca
     8461 attggttttg atgttttcgg aatttgatgt ttccaaaaat gttgtgtttc acctttcata
     8521 attaacaggc taccgtgttc taatgtaatt ttttgtttac actctttaat ttcattatgt
     8581 tgtaaatgaa aaactctttc tgctccaaaa ctaattgagg caattattgg atttcgacct
     8641 agttcttttt cattatctgc atgccatcca ttactgtctt taccgtcacg atattggttt
     8701 agtaaaactg ttgtgaaatt ttcaggacaa atttcctcca cttcattttt gataaacatt
     8761 aacaaaggat tccatttatg aggttgcatt acgatattag aataagaata tgttttacca
     8821 tcatttccat ataaagcggt caatcgaggt tgaggatatg tttgaccaaa aacagtaata
     8881 ttgtcttgtt gccaaggaat ttcatttttt aatttttgaa ataattcatt agatttttct
     8941 actgaaaata aatttggata atactcgatt tcagcgtcgg gtaaatcaaa tacaattttt
     9001 tctttgggaa ataatgaatt catttggtaa aaataaaaaa agcgatggaa aactccatcg
     9061 cttgaggtaa attaagaagt aaaattatta ctcttcattt ttttgtaaca catttttata
     9121 aataaatccg gcaacaattg ctcctaaaat tggggcaagc cagaaaagcc atagttgact
     9181 caaaggttct cctccaacaa aaatagcctg ggaagtagaa cgagccggat taacagatgt
     9241 atttgtaata ggaatactga ttagatgaat taaggttaaa cctaaaccga ttgcaattcc
     9301 ggcaaattta ccgtttgcaa atttatcagt tgctccaagt attattaata aaaagaacat
     9361 tgtcaataca aattctgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
     9481 gtacaaatgc caaggtaaat tcataaatga attgctttgt tcaatacgaa tttataaaaa
     9541 aaatgttaat aaaatgaaga acagtaaaat attttacaaa ataagcatta aatctttctt
     9601 atgaatttga tattcatgac aacaatcgca agaatgatta gaatgattcc aagccactgt
     9661 aaaccattta ctttttcact taaaaggaag taagccataa gaactgaaac cggtaattct
     9721 agcgaggaaa caatacttcc aagtccaatg cctgtcaaag gaaaaccggc atttaataac
     9781 attggtggaa tgatagtgcc aaaaagagca acaataattc cccattttaa aaagatttca
     9841 aaattaaatg gggttgattg tgtaactatt gaaaagagaa aaacaatgat tgctccccct
     9901 aaaagcatgt ataaacttcg ttgtgcagag gtaatttctg tggccacgcg attggctgta
     9961 aacatggtag tagtaaagga agcagctgct aaaattcccc aaaataatcc acgccaatct
    10021 atagcaattt cattttgaat taaatttgtt gctaaaactg ttccaattaa aacaatgaca
    10081 acagccatta ttttttgaat agaaggtagt tttttatcta aaatcatttc taacaataca
    10141 cccatccaaa cagtttgcat gagtaagaca atagcaatgg aaacatcgat atattttaca
    10201 cttaagtaat agaaaacact tgtcatacct aaggaagttc ctgccaacat taaatggaaa
    10261 atatttttac ctgttgcttt aaaggctatg tttttatttt taactcgttg gaaagaattg
    10321 ataagaagca cgccaatgat tccatataca aattgagctg tagtgacttc agcagtggta
    10381 aaattttcgt gataggctaa ttttacaaac gtagccaaca tgccataact tgtcgctcca
    10441 agggcaacta agaaaactcc tttcagtact cgatttccga acatatatga tttttaaaaa
    10501 gcgggcaaag ataagtaaag ttgaggttag aaggatacaa gttgtaagat tatataaaaa
    10561 tttaatatac taaataacaa tttaaatagt ttagaaatta aaaagaccaa tgcaaaaatt
    10621 atccttaatt atcttattaa gttttgtatg ttgtcaaaat acaattcatg aagtacctat
    10681 aaattataat tcttttcatc aagaagcatt gactttttgc aaacaaaata aatttaatca
    10741 ggatttttat ttttttgttg atttaagtat tcattccggt aaaaaccgct tttttattta
    10801 tgattttaaa aaccaaaaag tagttaatca aaatcttgta acgcatg
//
LOCUS       DAOTCW010000074        10810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3940_length_10810_cov_43.803161, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000074 DAOTCW010000000
VERSION     DAOTCW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10810
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3940_length_10810_cov_43.803161"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="P5335_07300"
     CDS             complement(<1..614)
                     /locus_tag="P5335_07300"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRZ74718.1"
                     /translation="MKIFLSFSVKTIKITTLFAYLFFCTMVFSQGETENEQLWIGYLT
                     QSKISKNYSLWNDTHWVPDNFYIIRTGLTYHFDNKLKTTTTLGYALAWFYPMEGKETF
                     KFEQRPWGQTTISHSSKSFNFFHRLRYEARFRSVIVADERTSDYDFNFRFRYLIQAKL
                     YFKNQPNSNRFYALVSDEIMFNAGDEITNEFRFDQNRIALGLGY"
     gene            673..2208
                     /locus_tag="P5335_07305"
     CDS             673..2208
                     /locus_tag="P5335_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRZ74719.1"
                     /translation="MLVKVFGSAVFGVEATTITVEVNIDKGVGYHLVGLPDNAIKESS
                     YRIAAALKNNGYSLPVKKITINMAPADLRKEGSAYDLTLAIGILVASGQIRADELDQY
                     VIMGELSLDGGLQPIKGALSIAIKAKEEGFKGFILPIQNVKEAAIVEGLDVYGIENVL
                     QVIDFFEGNGTIEPTIIDTKEEFSRTLDFPEFDFSDVKGQESIKRCMEIAAAGGHNII
                     LIGPPGSGKTMLAKRLPSILPPMTMKEALETTKIHSVAGKTKEAGLMSQRPFRSPHHT
                     ASSVSLVGGGSYPQPGEISLAHNGVLFLDELPEFKREVLEVMRQPLEDREVTISRAKF
                     TITYPSSFMLVASMNPSPSGYFNDPDAPISSSPAEMQRYLSKISGPLLDRIDIHIEVT
                     PVPFEKLTETRKAESSVEIRKRVTKARELQTVRFDSMPSIHYNAQMSSKLIREFCVLD
                     ETSLQLLKTAMERLNLSARAYDRILKVARTIADLEASELLKPTHIAEAIQYRSLDREG
                     WLG"
     gene            complement(2225..3580)
                     /locus_tag="P5335_07310"
     CDS             complement(2225..3580)
                     /locus_tag="P5335_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HRZ74720.1"
                     /translation="MNVGIDSISFDIPKIYLPISTLAIHRGIEPEKLTKGLGLQKMSF
                     PDANQDVVTFAANAALKLIQQEDCNPTTISRIYIGSESGVDSSKPIASYVLNLLESKL
                     GQGSFRNCDVVDITFACIGAVDALQNCLDYVRLNPIKKAIVIATDNAKYDLNSTGEYT
                     QGAGAIAMLISSNPRILSFSNEVGVSTEGVFDFFKPRRYFSKEEITKKNDNPEWLNVL
                     ENEVTIYKEQPVFDGQYSNECYINRITEAYTHFKNESQQSGKIYKDWSSILMHLPYCF
                     QGRRTFVEIFSKENPELLTNQLGETEKDKLKALTKSPEYLELINQKIYPSEIASGQVG
                     NIYTGSIFLGLLSTLSYHVQQNTTLTNQKVGFIAYGSGSKSKVFEAIISPDWKSQIEK
                     LSLFENLKNCFAIDFETYEKLHKKELKQSVLKPKNEFVLDFIENENPVLIGARYYKFV
                     D"
     gene            complement(3733..4590)
                     /locus_tag="P5335_07315"
     CDS             complement(3733..4590)
                     /locus_tag="P5335_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRZ74721.1"
                     /translation="MKWEGRRQSSNMEDRRGMGTTGKVVAGGGLIGIVFLVIQMFMGG
                     GDNAQLMQELQNQMQQQQTETGPAQPLSEADQKMGEFVATVLADTEDVWHKIFEENGI
                     KYVEPKMVLFRGSVQTACGGASSASGPFYCPGDQKVYMDLDFFDELRTRFGAKGGDYA
                     IAYVIAHEVGHHVQTLLGTSDEVRKLQQKVSDVEGNKLSVCLELQADFYAGLWTHYNQ
                     KYLEEGDIEEALSAAQAVGDDAIQKKMQGHVVPDSFTHGTSEQRVKWFGKGFKTGDIK
                     QGDTFATLN"
     gene            complement(4634..>6727)
                     /locus_tag="P5335_07320"
     CDS             complement(4634..>6727)
                     /locus_tag="P5335_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRZ74722.1"
                     /translation="KKIEDTLQNKSNVLTAITKISEKILANSSVFNVFDEILETVGTV
                     TNIDRVYYFEVNEEKRVISQKYEWTNSKTEPQIDNEELQNLPFEVILDMIIPLKKNKN
                     YNKIVKDIFESDLKEMLKSQNIKSILILPIFVKNAFYGMIGFDDCTNERLWTEDELNI
                     LQTLVNNITSAIERNINENIINDSEIRFRLLADNIPGTIYLSKYDSKFTKIYLNNEIE
                     NLTGYPKEDFLTNEISYLDIIHPDDRERVIKDQKYALENGQKIHFIYRIIHKDDRIVW
                     VEEFGDVIVKDNQVEFIEGIFIDITERKLQEAAIKEKEMAIAANKAKSEFLANMSHEI
                     RTPLNGIIGFTDLLMRSDLEKSQAKYMKTVNQSAKSLMSVINDILDFSKIESGNLELV
                     IEETKLTELAREVIDTIKFDAIQKKIDVDLHINKDVPVKVWIDPIRLKQILINLLGNA
                     VKFTSNGKIKLTVTVLDRITTHQTRLRFSVIDTGIGIRRENQAKIFEAFSQEDSSTTR
                     QYGGTGLGLSISNKLLALMNSKLQLDSEPNKGSTFYFDIDVQSSSKEIENMPKRIEVD
                     EEYVYQNQSNANVLIVEDNNINSLLAKTLIKKIIPNAKITTVTNGLEAYEKYQEATFD
                     IIFMDIQMPIMNGYEATKEIRKLDTGKEVPIIALTAGTVIGEKEKCLEIGMNDYVSKP
                     IIKGSLEEIIAKWVK"
     assembly_gap    6728..6827
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6828..8326)
                     /locus_tag="P5335_07325"
     CDS             complement(<6828..8326)
                     /locus_tag="P5335_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRZ74723.1"
                     /translation="MPSSFSLKLFSFILVNFISSAVYSATTNRNVSDNEIVAFESFKK
                     YYWLLVILIVLVTTMFLQLNKKRSKFSKDYTANYSDITNDAYLYRLYFLFFGILIPSV
                     DTVFEVLKIRSYSLIIQSYSFGAVFLIIYFLSYKIKKIENNLSKLFTISFLIFSIIVL
                     TNLVTRPFELVTFSGMLIIVLFSFNVFNNYKHYLLYIVLIITVIIGLIIQKQIPLSIG
                     IITIIFCLIIVIINQTKHVALQNNKEKFLFANEIVNRGKSLVIAAKKSGELTYCSESI
                     QNILGYNHVEVLGMGFWELTEDPEFVGESYHDTYMVDRMYTRKLKCKDGTYKYIQWVD
                     KQFGEDLFVGIGNDVTDQINIEHRYQNLIESATDLIFETDDDGKFTFINSFSCSLLEY
                     DLTELMNMYYAELIREDYREQIVSHYQEEFKYLNDFSLLEFPILKKSGEEVWISQKVT
                     IKRNDLNEIIGYSGIARDITMIRNLEVEKSQRQDKIKKYNNALSKLSTT"
     gene            complement(8426..9211)
                     /locus_tag="P5335_07330"
     CDS             complement(8426..9211)
                     /locus_tag="P5335_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74724.1"
                     /translation="MENKDTQIKHAITFGYPLDFGLVIEKSFENYKKIALLGGATILL
                     LMIALIGIGFTMFTAFMGVSDLTETLTDFNPLNFSTVGILIYIGVMVLISGLFSPIGA
                     GFLKMAHLAEENKEFTMGTVFDYYKTIHFKELFISASLLAVVNLGISTTLDSMSLTVL
                     GTILTYIIGFLTFLTVPLIIFANQNAFEAITKSAQLIIKNPFIIFGLIIVGIIGIFIG
                     LIGLCIGVFFTMPFWYSLTYTIYNNIVPINEESEIEEIGNNLE"
     gene            complement(9222..10550)
                     /locus_tag="P5335_07335"
     CDS             complement(9222..10550)
                     /locus_tag="P5335_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HRZ74725.1"
                     /translation="MSILVSPQKRKKLLKKLLISIQNHESAIIEALQKDFKKPPFETY
                     ITEINYVISDLKHTISNVENWSKPKRVWPSILNFPSFDYIYTEPYGRVLIISPWNYPF
                     QLAMCPLIAAIAAGNTIVLKPSELTFNTSQLITKIVEEVFDVKEVVVVHGGAEFTQSL
                     LEKRWDYIFFTGSVFVGKIVAQAAAKNLTPYTLELGGKNPCIVDEGADLEIAVKRIVW
                     GKFINAGQTCIAPDYILVQGKEKYNFVELLKKEIIRVYGENPEESKDFSRIINSKNWH
                     RLNEMIDHTKVLFGGITNENEKYISPTLLDEPSLNSEVMKDEIFGPILPIISYHDEKD
                     LENIISKYEKPLALYAFTNDKKFAERMITKFSFGGGCVNDCMVHFSNHRLPFGGVGHS
                     GIGAYHGKLGFDSFSHQKAIVKKPNWADNPIRYAPYKNKLNFIKKVLSWL"
     gene            complement(10575..>10810)
                     /locus_tag="P5335_07340"
     CDS             complement(10575..>10810)
                     /locus_tag="P5335_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRZ74726.1"
                     /translation="YFVLEIELHTGRHHQIRAQLQTIGCPIKGDLKYGFDRSNPDGGI
                     HLHARKLCFIHPVSKEKIELIAPTPNDIIWNSI"
BASE COUNT         3479 a         1752 c         1677 g         3802 t
ORIGIN      
        1 tgataaccca atcctaatgc tattctattt tgatcaaaac ggaattcgtt tgttatttca
       61 tctcctgcat taaacataat ttcatcagag actaaggcat aaaatcgatt actattcggt
      121 tggtttttaa aatataactt tgcttgaata aggtatctaa atcgaaaatt aaaatcataa
      181 tcacttgttc tttcatcggc aacaataaca cttctaaagc gggcttcata acgcaaacga
      241 tgaaaaaagt tgaatgattt tgaagaatga ctaatggtag tttgtcccca aggtctttgt
      301 tcaaatttaa aagtttcttt accttccatc ggatagaacc atgctaatgc atatcccaaa
      361 gttgttgttg ttttgagttt attgtcaaaa tgataggtta aacccgttct aatgatataa
      421 aaattatctg gaacccaatg ggtgtcgttc cacaacgagt agttctttga aatttttgat
      481 tgggtgagat aaccaatcca tagttgttcg ttttcagttt cgccttgtga aaataccatt
      541 gtacaaaaaa ataaataggc aaatagagtt gttattttaa ttgtcttcac ggagaatgat
      601 aaaaatattt tcaaaaatac tattttctta tcattctatt tgtgatattt gtcatagata
      661 aatttatacg ccatgttagt taaagtattt ggaagtgccg ttttcggtgt ggaagccacc
      721 acgattaccg tagaagtaaa tattgataaa ggagtaggct accacttagt aggattaccc
      781 gacaatgcca ttaaagaaag tagttatcga atagctgctg ctctcaaaaa taacgggtat
      841 tccttaccgg ttaaaaaaat caccatcaac atggctccgg ccgatttaag aaaggaaggc
      901 tccgcttatg accttacttt ggcaattggt attttagttg cttcaggaca aattagagcg
      961 gatgagttag accaatatgt gattatggga gaattatctc tagatggcgg tttgcaacct
     1021 attaaaggag ctttgtcaat agccataaaa gcgaaagaag aaggttttaa aggttttatt
     1081 cttcccattc aaaatgtgaa agaagctgca atagtggaag gtctggacgt ttatggaatt
     1141 gaaaatgtat tacaagtcat tgattttttt gaaggaaatg gaactattga acctacaatt
     1201 atcgatacca aagaagagtt ttccagaaca ttagattttc cggaatttga tttttccgat
     1261 gtgaaaggtc aggaaagtat taagcgttgt atggaaattg ctgctgccgg aggacataac
     1321 atcattttaa ttggtccgcc gggttcgggt aaaacaatgt tggcaaaaag attgccgagt
     1381 attttaccgc ctatgaccat gaaagaagca ttggaaacta ctaaaataca ttctgtagcc
     1441 ggaaaaacaa aagaagcagg attaatgagc caacggccat ttcggagtcc gcatcacact
     1501 gcatcctctg tttcattagt tggtggaggg agttatccgc aacctggaga aatatcttta
     1561 gctcataatg gtgttttgtt tttggatgaa ttacctgaat ttaaacggga agttttagaa
     1621 gtcatgcgtc aacctttaga ggatagagaa gtaacgattt ctagagcaaa atttacgatt
     1681 acctatcctt cttcctttat gttagttgcc agtatgaatc cgagtccgag tggttatttt
     1741 aatgatccgg atgctccaat aagttcatca ccggctgaaa tgcaacggta tttgagtaaa
     1801 atttccggtc ctctattaga taggattgat attcatatag aagtaacacc cgttccattt
     1861 gaaaaattaa ccgaaaccag aaaagcggaa agcagcgttg aaataagaaa acgtgttacc
     1921 aaagcgagag agttacaaac agtcaggttt gattctatgc cttcgattca ttacaatgct
     1981 caaatgagta gtaaactgat tcgtgagttt tgtgtgttgg atgagacctc attacaatta
     2041 ctgaaaacag caatggaaag attaaattta tctgccagag cttatgatag aatattaaaa
     2101 gtagcccgaa ctatagctga tttagaagca tcagaattgc ttaaaccgac acatattgcc
     2161 gaggcaattc agtatagaag tttggatagg gaaggttggt tgggataaat tacaagtaat
     2221 ttttttaatc cacaaacttg taatatcgtg ctccaataag tactgggttt tcattttcga
     2281 tgaaatctaa aacaaactca tttttgggtt ttaaaaccga ttgttttaat tcttttttat
     2341 gtagtttttc ataggtttcg aagtcaatag cgaaacaatt tttgaggttt tcaaaaagag
     2401 aaagtttttc aatttgtgat ttccaatcag gagaaataat tgcttcgaaa acctttgatt
     2461 ttgacccact accataagca ataaagccaa ctttttgatt ggttaatgtt gtattttgtt
     2521 gaacatgata acttaacgta gaaagtagtc ctaaaaagat tgaacctgtg taaatattac
     2581 ctacctgtcc ggaagcaatt tcggagggat agattttttg gttgataagt tctaaatatt
     2641 ccggactttt agttagggct tttaatttgt ccttttctgt ttcacccaat tgattggtta
     2701 acaattccgg attttctttg gaaaaaattt cgacgaatgt tcttctgcct tgaaaacaat
     2761 agggtagatg catcaaaatg gatgaccagt ctttatagat ttttccactt tgttgacttt
     2821 catttttaaa atgagtgtag gcttccgtaa ttctgttaat atagcattca ttagaatatt
     2881 ggccatcaaa aacgggttgc tctttgtaaa tggtaacttc gttttctaac acatttaacc
     2941 attccggatt atcatttttt ttggtaattt cttctttaga aaaatagcgg cgtggtttaa
     3001 agaaatcaaa aacaccttca gtagaaacac ccacttcatt tgaaaaactt aaaatcctag
     3061 gatttgaaga aattaacatc gctatagctc cggcaccttg tgtatattct ccagtggagt
     3121 ttaaatcgta tttggcatta tctgtggcta ttacaattgc ttttttaata ggatttagtc
     3181 taacatagtc caaacaattt tgtaatgcat ctacagctcc aatacaagca aaagttatat
     3241 ctaccacatc acaattgcgg aatgaacctt ggcctagttt actttctaac aaattaagta
     3301 cgtaagaggc aattggtttt gaactatcaa caccactttc actacctata tatatccttg
     3361 aaattgttgt tggattacaa tcttcctgct gaattagttt taaggctgca tttgctgcaa
     3421 aagtaactac atcttggtta gcatccggga aactcatttt ttgtaaaccc aagcctttag
     3481 tgagtttttc aggctcaata cctcgatgta ttgctaaagt ggaaataggt aaataaattt
     3541 taggaatatc gaaagatatg ctgtctatac caacgttcat gttgaaaaat ttgatggaca
     3601 aaattaaaaa agagcctttc aataattgat aaacaattca taaaaaatct cttttttaaa
     3661 acgaattacg aatgttttaa aaaagggaag ttattatttt gaaaagaaca attttttagt
     3721 ctaaaactga ttttaattca atgttgcaaa ggtatcgcct tgtttgatat cacctgtttt
     3781 aaaaccttta ccaaaccatt ttacacgttg ttcagaagtt ccgtgagtaa aagaatctgg
     3841 aacaacatgg ccttgcatct tcttttgaat ggcatcatca ccaactgctt gagctgcact
     3901 taaagcttct tcgatatcgc cttcttctag gtatttttgg ttataatgtg tccataaacc
     3961 ggcataaaaa tcagcttgaa gctctaaaca tacagataat ttattacctt ccacatcact
     4021 tactttttgt tgtagtttac gaacttcatc agaagttcct aatagtgttt gtacatggtg
     4081 acctacttca tgagcaatca cataggcaat ggcataatcg ccgccttttg caccaaaacg
     4141 ggttcggagt tcatcaaaaa agtctaaatc catatatact ttttgatctc cggggcaata
     4201 aaaagggcct gaggcagacg aagctccacc gcaagctgtt tgaacagagc cacgaaataa
     4261 aaccattttt ggttcaacat attttatacc attttcttca aatattttat gccaaacatc
     4321 ttctgtatcg gctaaaaccg tggccacaaa ttctcccatt ttttggtcgg cttcacttaa
     4381 aggttgagcg ggaccggttt ctgtttgctg ctgttgcatt tggttttgca attcttgcat
     4441 caattgggca ttgtcacctc cacccataaa catttgtatg acaaggaata caataccaat
     4501 taaacctcca ccggcaacca cttttcctgt ggttcccatt cctcttcgat cttccatgtt
     4561 tgaactttgt cttctacctt cccatttcat agtgttgaat ttttgattta tataaaagta
     4621 aaaaaagtta ttattatttc acccatttag caattatttc ttctaaactt ccttttataa
     4681 tcggtttaga aacataatca ttcattccga tttcaagaca tttttctttt tcaccaataa
     4741 cagtaccggc agttaaggca ataatgggaa cttcttttcc ggtatctaat tttctaattt
     4801 ctttggttgc ttcataaccg ttcataatgg gcatttgaat atccataaaa ataatatcaa
     4861 aagtggcttc ttgatatttt tcataggctt ctaaaccatt tgtaacggta gtaatttttg
     4921 cattcggaat tatttttttt attaatgttt ttgccaaaag tgaattaata ttgttgtctt
     4981 caacaatcaa cacgtttgca ttggattgat tttgataaac atattcttca tctacctcta
     5041 ttcttttggg catgttttcg atttctttgc tggaagattg aacatcaata tcaaaataga
     5101 aagtacttcc tttgtttggt tcactatcta attgtagttt gctattcatc aaggcaagaa
     5161 gtttgtttga tatacttaat cccaaacctg ttccaccata ttgtctggtt gttgaactgt
     5221 cttcttgtga aaaggcttca aatattttgg cttgattttc tcgtctgatt ccaataccgg
     5281 tatcaatgac tgaaaaacgt aaacgcgttt gatgagtagt aattctgtca agtacggtta
     5341 cggttaattt aatttttccg tttgaggtaa atttcacagc atttcccaat aaatttatta
     5401 aaatttgttt taatctaatt gggtcaatcc aaacttttac cgggacatct ttgtttatat
     5461 gcaaatcaac atcaattttc ttttgaatag catcaaactt aattgtatct atcacttccc
     5521 gagcaagttc agttaatttt gtttcttcaa taactaattc tagatttccg gattcaattt
     5581 tagaaaaatc cagtatatcg tttattacac tcattaacga tttagcagat tgattaaccg
     5641 ttttcatgta ttttgcttgc gatttttcca aatcagaacg catcaataaa tcagtaaaac
     5701 caataattcc atttaaagga gttctgattt catgactcat attggctaaa aattctgatt
     5761 tagctttgtt agccgcgatt gccatttctt tttctttaat ggctgcttct tgtaattttc
     5821 gttccgtaat gtcaataaaa ataccttcta taaattctac ttgattatct tttacaatta
     5881 catcaccaaa ttcttcaacc caaacaatac gatcgtcttt gtgaataatt cggtaaataa
     5941 aatgaatttt ttgaccattt tctaatgcat atttttggtc ttttatcact ctttctctat
     6001 cgtcggggtg aataatatct aaataggata tttcgttagt taaaaaatct tcttttggat
     6061 atccggttaa attttctatt tcattgttca aataaatttt tgtaaatttt gaatcatatt
     6121 ttgaaagata aatggtaccc ggaatattat cagctaaaag tctgaaacgg atttcactgt
     6181 cgtttatgat attttcatta atatttcgtt caatagccga agtaatatta ttaactaaag
     6241 tttgaagaat atttaattcg tcttccgtcc acaatctctc gttggtacaa tcatcaaatc
     6301 caatcatccc ataaaatgcg tttttaacga aaataggtaa tattaaaatt gattttatgt
     6361 tttgtgattt aagcatttct tttaaatcac tttcaaagat atcttttaca attttattgt
     6421 aatttttatt ttttttgagt ggaataatca tatccaatat cacttcgaaa ggtaaatttt
     6481 ggagctcctc attatcaatt tgaggttctg tttttgaatt tgtccattcg tatttttgag
     6541 aaatgactct tttttcttca tttacttcaa aataatatac cctgtcaata ttggtaactg
     6601 taccaactgt ttccaaaatt tcatcaaaaa cattaaaaac acttgaattg gctagaattt
     6661 tttcggaaat ttttgtgatt gcagttaata cattactttt attttgaagt gtatcttcaa
     6721 tttttttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctc gttgtagaca
     6841 attttgaaag tgcattatta tattttttga ttttgtcctg acgttgtgat ttttcaactt
     6901 ctaagtttcg aatcatggta atgtcacgag caatccctga atagccaatg atttcattta
     6961 gatcatttct tttgatagtt actttttgag aaatccaaac ttcttcacca cttttcttta
     7021 gaattggaaa ttcaagaagt gaaaaatcgt ttaaatattt gaattcttct tgataatgac
     7081 ttactatttg ttccctatag tcttcccgaa ttaattcagc ataatacatg ttcatcagtt
     7141 cagttaaatc atattctaac agtgaacagg aaaaggagtt tataaaggta aatttaccat
     7201 cgtcatccgt ttcgaaaatt aaatcggtag cactttcaat taaattttga taccgatgtt
     7261 caatattaat ttggtctgta acatcattgc caataccaac aaaaaggtct tctccaaatt
     7321 gtttatcaac ccattgaatg tatttatatg ttccgtcttt acattttaat ttccgggtat
     7381 acattctgtc aaccatatag gtgtcatgat aggattcccc aacaaattcg ggatcttcag
     7441 tgagctccca aaaacccatt cctaaaacct cgacatgatt atagcctaag atattttgta
     7501 tgctttcact acagtaggtt agttctcctg atttttttgc tgcaattact aatgatttac
     7561 cacgattgac tatttcattc gcaaagagga atttttcttt gttattctgt aatgcaacgt
     7621 gttttgtttg gttaataatg acaataatca aacagaaaat gatggtaatg attcctatac
     7681 ttaatggaat ttgtttttgt atgattagtc caataattac agtaattatg agaactatgt
     7741 atagtaaata gtgtttgtaa ttattgaaaa cattaaagga aaataaaaca ataattaaca
     7801 ttcctgaaaa agttactaac tcgaaaggtc tagttactaa atttgtcaat acaataattg
     7861 aaaaaatgag aaaactaatt gtaaatagtt ttgagagatt gttttctatt tttttgattt
     7921 tataactcaa aaaataaatt attaaaaaca cagccccaaa actataactt tggataatta
     7981 gtgaatagct cctgattttt aatacttcaa aaacggtatc aacagaagga attaaaatcc
     8041 caaaaaataa aaaatacaag cgatagaggt atgcatcgtt tgttatgtca gaataatttg
     8101 cagtgtaatc tttagaaaat tttgaacgtt ttttatttaa ttgtaaaaac attgtggtta
     8161 ctaagacaat caatataacc aacaaccaat aatatttctt aaaactttca aacgcaacta
     8221 tttcattatc agatacgtta cgatttgttg ttgctgaata gacagcagaa gaaataaagt
     8281 ttactaaaat aaaagaaaac aatttcagag aaaatgatga gggcatttgt ttgtttgggt
     8341 ttgtttttca agtctaaaaa tataaaaaaa agagttgcat actaactaca actctttttt
     8401 tattgatttt ttttatttta aatagttatt ctaggttgtt tccaatttcc tcaatttcac
     8461 tttcttcatt tataggaacg atattattgt agatagtata agttaaagag taccaaaatg
     8521 gcattgtgaa aaagactccg atacacaacc caattagtcc aataaatatt ccaataatcc
     8581 cgacaataat taatccgaaa attataaaag ggttttttat aattaattgt gcactttttg
     8641 ttattgcctc aaaagcattt tgatttgcaa agatgataag tggaacggtt aaaaaagtta
     8701 aaaatccaat gatgtaagtt aatatcgtac ctaaaacagt taaactcata gaatctaaag
     8761 ttgtactgat tcctaaattc acgactgcta atagtgaagc agaaataaat aattctttaa
     8821 aatgaattgt tttataatag tcaaaaacag tccccatggt gaactcttta ttttcctcag
     8881 ctaaatgagc catttttaaa aaaccggcac caattggact aaaaagacct gaaatcagaa
     8941 ccattactcc aatgtaaatt aagattccaa cagtagaaaa atttagcgga ttaaaatcgg
     9001 tcaaggtttc agttaaatcg gatactccca taaatgctgt gaacatagtg aatccgatac
     9061 caatcaaggc tatcatcaat aataatattg ttgcaccccc aagtaaggca atttttttat
     9121 aattttcaaa agatttttca ataactaatc caaaatcaag aggataacca aatgtaatgg
     9181 catgtttaat ttgcgtgtct ttattttcca tacgttatat tttagagcca agataaaact
     9241 ttttttataa aattcaattt gtttttataa ggagcatatc gaatagggtt atctgcccaa
     9301 ttcggttttt ttacaatcgc tttttgatga gaaaaagaat caaaacctaa tttaccatga
     9361 tatgcaccta ttccactatg tccaacccct ccaaaaggca atcgatgatt ggaaaaatga
     9421 accatgcaat catttacaca acctccaccg aatgagaatt ttgtaatcat tctttctgca
     9481 aattttttat cgtttgtgaa agcgtataat gctaatggtt tttcatattt tgaaattata
     9541 ttttctaaat ctttttcatc atgataagat ataattggta aaatagggcc aaaaatttca
     9601 tctttcatta cctcactatt caggctgggt tcgtcaagta atgttggact tatgtatttt
     9661 tcgttttcat ttgttatccc accaaaaagc actttagtgt ggtctatcat ttcatttaaa
     9721 cgatgccaat ttttagaatt gattatacgt gaaaaatctt ttgattcttc tggattctca
     9781 ccatacactc gaataatttc tttttttaat aattctacaa aattgtattt ttcttttcct
     9841 tgtactaaaa tataatcagg tgcaatacaa gtttgtccgg cattaatgaa tttaccccaa
     9901 acaattcgtt ttactgcaat ttctaaatca gctccttcat caacaatgca agggtttttc
     9961 ccacccaatt ctagtgtgta aggtgttagg ttttttgcag cggcttgagc cacaatctta
    10021 ccaacaaaca cactaccggt aaagaagata taatcccacc gtttttctaa taaagactgg
    10081 gtgaattcag ctccgccatg aactacaact acttctttta cgtcaaaaac ttcctcaaca
    10141 atttttgtaa taagctggga tgtattaaag gttaattcgg atggttttag aacaatagta
    10201 tttccggcag caatagcagc aattagtgga cacatagcta actgaaatgg gtaattccac
    10261 ggcgaaataa taagtactct accataaggt tccgtataaa tataatcgaa agaaggaaaa
    10321 ttcaaaatag agggccaaac ccgttttggt ttagaccaat tttcaacatt tgaaatcgta
    10381 tgttttaaat cagaaattac ataattgatt tctgtaatat aggtttcaaa aggaggtttt
    10441 ttaaaatctt tttgtaaggc ttcaataatt gctgattcat gattttgaat agaaatcagt
    10501 agctttttga gcaatttttt tcttttttgg ggagagacga gtatactcat aacttgataa
    10561 tcaaaaatta aactttatat cgaattccaa ataatatcat tgggggtagg agcaattaat
    10621 tctatttttt ctttagaaac ggggtgaata aaacacaatt ttctggcatg tagatgaatt
    10681 ccaccgtccg gattacttct gtcaaaacca tattttaaat ctccttttat aggacaacca
    10741 atggtttgta attgtgccct aatttggtga tgtctaccgg tatgtaattc aatttctaaa
    10801 acaaaataat
//
LOCUS       DAOTCW010000075        10766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_3970_length_10766_cov_38.538325, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000075 DAOTCW010000000
VERSION     DAOTCW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10766
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3970_length_10766_cov_38.538325"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="P5335_07345"
     CDS             complement(<1..215)
                     /locus_tag="P5335_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRZ74727.1"
                     /translation="MKFIVSSSYLLKQLQVLGSVINSSNTLPILDNFLFELDNNRLIV
                     SASDLETTMSATLEIDSTSQGSVAVPA"
     gene            complement(288..1952)
                     /gene="gldG"
                     /locus_tag="P5335_07350"
     CDS             complement(288..1952)
                     /gene="gldG"
                     /locus_tag="P5335_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HRZ74728.1"
                     /translation="MKTQTKKNLQQLFLLFMLVIILNIIGNFYFKRFDLTHDKRYTLS
                     ETTIQLIKEIKEPIFIDVFLDGQFPPEFKRLQNETKQILEEFRSYNSNLIFQFNNPIE
                     DESKAEQYVQELVSLGFTPTNINSNKNGKKELIQIFPWAIANQGQKSVRVPLLVNNFG
                     NSPSENITKSVQLLEFAFADALTKITTGKQKKIAVLKGNGEIADKYQADFLLNLKEYY
                     QLGEFNLDSLQENPQKILENLNRFDAAIIAKPTEAFSDKEKYILDQYVMNGGKSMWLI
                     DKVVMDIDSLQTENNSALAYPRELNLDDLFFKYGIRINYQLVQDLLSTPITAQSAQGD
                     VPIDWLYSPIIKSEDNHSITKNINLVKLEFANTIDTLKNGIKKTVLLKSSAQSKAVGA
                     PLEVNLYQFMDNLNEEDFSKGNQTIGVLLEGKFNSAFKNRVKPFTLTKELDSGIENKM
                     IVIADGDIVNYNYVNKKPLLNGIDQWTQQVYGNKEFLLNAMNYLLDDTGLINIRNKEI
                     TLAFLDKDKVSKNYSQIQIITVGLPLIVLLLFGVLFFFIRKKKYAK"
     gene            complement(1952..2677)
                     /gene="gldF"
                     /locus_tag="P5335_07355"
     CDS             complement(1952..2677)
                     /gene="gldF"
                     /locus_tag="P5335_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HRZ74729.1"
                     /translation="MKAILFREIKTFFGSPIGYLVIAIFLLLNGLFLWVFEGDFNIAN
                     SGFADLNPFFTLAPWILIFLIPAVTMRSFSDEKKQGTIELLLTKPLTSWQIVNGKFLG
                     AFLLIIFALVPTLVYVFVVSKYGLPEGNLDLGTTIGSYLGLLFLVASYTAIGIFTSTL
                     SENQIVAFILSVFLCFFFYFGFEGIAGFTASFADVVSAIGMDYHYKSISRGVIDTRDV
                     LYFSSVTVLFLAGTVYQLKSLKN"
     assembly_gap    2692..2791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2792..3088)
                     /locus_tag="P5335_07360"
     CDS             complement(2792..3088)
                     /locus_tag="P5335_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRZ74730.1"
                     /translation="MFVRIVKMSFHTEHIPTFLENFELMKEKIRNAPGNRLLELYQDK
                     NNPELFFTYSYWETEIDLENYRNSELFYDVWQFTKQLFNAKPEAWSVNKLVSLV"
     gene            complement(3095..3922)
                     /locus_tag="P5335_07365"
     CDS             complement(3095..3922)
                     /locus_tag="P5335_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRZ74731.1"
                     /translation="MSIITLTTDYGLKDHFVGALKGKIISEFPEAKIIDISHEIDPFN
                     IAEASYILQASFANFPKGTVHLIGVDAERNTENEHLAMQWNDQFFICADNGILSMLTQ
                     RILPQKIVAINIHDRLPENATDMDVFKTVACHLAKGGTLNVIGKEITELKSITDFQPI
                     VSKDGNSIKGNVIYIDHFGNIVFNISKKQFLELSKGRTYEIVMKPKSIKTILPNYSSV
                     AISDKFPIKYYEGEKLAIFNEAGFLEIAIFRSNPKTVGSAASLLGFSYRDTITINFK"
     gene            4029..>4978
                     /locus_tag="P5335_07370"
     CDS             4029..>4978
                     /locus_tag="P5335_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRZ74732.1"
                     /translation="MNERIIELHDIAPKEFWGAQDSHLETIKKYYPKLKIVARGTTLK
                     AYGEKEVLDEFENRFNRLMLHFTRYNNIDTNVIDRVIQSNSAEEHKMHDHDKILVHGI
                     GGKLIKAMTPNQQLMVDLIHKNDMVFAVGPAGTGKTYTGVALAVKALKEKQVKRIILT
                     RPAVEAGENLGFLPGDMKEKLDPYMQPLYDALRDMLPPQTLEDYILKGIIQIAPLAFM
                     RGRTLDNAFVILDEAQNTTHSQMKMFLTRMGKNAKFIITGDPGQVDLPRRTISGLKEA
                     ILILKDVDGIGILYLDDKDIVRHRLVKKVIDAYKSIENLD"
     assembly_gap    4979..5078
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5088..6047
                     /locus_tag="P5335_07375"
     CDS             5088..6047
                     /locus_tag="P5335_07375"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRZ74733.1"
                     /translation="MNTITTTNFQFPNQKSVYHGKVREVYNINDEILVMIATDRLSAF
                     DVVMPKGIPYKGQILNQIATKFMQLTADIVPNWLIATPDPNVAVGHLCEPFKVEMVIR
                     GYLSGHAAREYALGKRILCGVQMPDGLKENDQFPPETTGSIITPTTKAANGSHDEDIS
                     REAILEQGIVSEEDYLVLEKYTRALYQRGTEIAASRGLILVDTKYEFGKTKEGQIVLI
                     DEIHTPDSSRYFYLEGYQERQKSNEEQKQLSKEFVRRWLIENGFQGKEGQQIPNMSEA
                     YIETVSERYIELYENILGEKFVKADISNIQERIEKNVLAYLTK"
     gene            6214..6606
                     /locus_tag="P5335_07380"
     CDS             6214..6606
                     /locus_tag="P5335_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRZ74734.1"
                     /translation="MKKSVIYLGIALLGFGTFANAFNGSEKTPTKEVGIYAKYYATPL
                     CVAISKGEVEVVRKFIEYGANVNEKTNGLTPLMYAARYNQVEIIKILLEKGADVKVKD
                     DKGFTALDHAENSKATEAVAVLKNYLNS"
     gene            complement(6661..7932)
                     /locus_tag="P5335_07385"
     CDS             complement(6661..7932)
                     /locus_tag="P5335_07385"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRZ74735.1"
                     /translation="MTVDLLLGLQWGDEGKGKIVDVLTSKYDIIARFQGGPNAGHTLE
                     FDGIKHVLRTIPSGIFHKNAMNIIGNGVVIDPVVFQKELEGLEKFNMDIKSKLLISRK
                     AHLILPTHRLLDAASEASKGKAKIGSTLKGIGPTYMDKTGRNGIRVGDIEMADFKERY
                     RALADKHEAMISFYDVNLQYNLPEMEEEFFASIEVLKSLQFIDSEEYLNQALKSGKTI
                     LCEGAQGSLLDVDFGTYPFVTSSNTTAAGACTGLGIAPNKVKNVFGIFKAYTTRVGSG
                     PFPTEDFEEAGDVMAKVGNEFGSVTGRKRRCGWLDLVALKYAVQVNGVTGLYMMKGDV
                     LSGFKTLKVCTAYQYKGQKITHFPYNIEPENVEPVYTEMKGWQADLTGMTSYDELPAE
                     LKTYIEFIEKEIEVPIKVVSVGPDRTQTILK"
     gene            complement(7969..8415)
                     /locus_tag="P5335_07390"
     CDS             complement(7969..8415)
                     /locus_tag="P5335_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRZ74736.1"
                     /translation="METIKNQEVVKNVFTKYLEQKGHRKTPERYAILQEIYNNKEHFD
                     IESLYIKMKNKNYRVSRATLYNTIELLLECGLVRRHQFGQNQAHYEKSYFDKQHDHII
                     MTDTGEVIEFCDPRIQQIKQTIEDIFGIEIHNHSLYLYGNKKAIKE"
     gene            complement(8512..10737)
                     /locus_tag="P5335_07395"
     CDS             complement(8512..10737)
                     /locus_tag="P5335_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HRZ74737.1"
                     /translation="MTELEIEQENKAIAKEYKELLRISYQTLTPEDKKIIRKAFDVAV
                     DAHKDQRRKSGEAYIFHPIAVAKIVAAEIGLGATAIAAALMHDVVEDSPITVHEIEKM
                     FNPKIAQLVDGLTKIAKVKTDQEISMQAENFRKMLLTLNDDVRVILIKLADRLHNMQT
                     MESMVDYKQAKIASETLYIYAPLAHRLGLYNIKSKLEDLGLKYTEPDVYADIVSKMKE
                     SKEEQEAYITSISDVLKKSLNDEGIEYTIKGRPKSIYSIRRKMLAQGVSFDEVYDKFA
                     LRIVYKSNQHDEKFVAWKIYSIVTDHYRPSPSRLRDWISSPKSTGYEALHITVMGPKG
                     RWVEIQVRSERMDEIAEKGYAAHYKYKQGISEENTLDTWLNLLKEALENSTSNAVDFV
                     EDFKLNLYSKEIYVFTPKGEIKSLPKGATSLDFAFSIHSEIGVRTRGTRVNGKLVPLN
                     HVLSSGDQIEIITSPNQKPNSHWLDYVTTSRAKNKIKNVLNEDTKKIAEDGKELLNRK
                     LRHLKITLNETTTNELVSYFKLKTSLDLFYRIGLGTIDNQQLKDYAAQKSNTLINFFK
                     KIKRSPSVAPEQIQKNELSKNFDMLVFGAEQDKLDYKLSSCCNPIPGDDVFGFLTIND
                     GIKIHKKDCPNAISLQSNYAYRIIQARWIDSSQEDFKAVLRITGMDSLGLTNELTRVI
                     SNQMNVNIQSISLSGDAGIFNGQVTVVVQNNTILKRLIENIKKIDGIDKVTRVNNAKL
                     E"
BASE COUNT         3152 a         1771 c         2036 g         3607 t
ORIGIN      
        1 tttgccggta cagcaacgct tccctgactg gtgctgtcaa tttctaaagt agcagacata
       61 gtagtttcta aatcagaagc agaaacaatc aaacgattat tgtctaattc aaaaaggaaa
      121 ttatctaaaa taggtaaggt attgctgctg ttaattacac tacctaaaac ttgtaattgt
      181 tttaataaat atgaactcga tactataaac ttcatttcgg tttttatttt tatatcccac
      241 aaatataaag gtaactattc aaacaaaaaa atgtatttta ttaacatcta ctttgcgtat
      301 tttttctttc tgataaaaaa gaacaataca ccaaataaaa ggagtacaat cagtggtaat
      361 ccaacagtta taatctgaat ttgggaataa tttttactga ctttatcttt gtccaagaaa
      421 gccaatgtaa tctccttgtt tctaatgtta ataagtccgg tatcgtctag taaataattc
      481 atggcattga gcagaaattc tttgttgcca taaacttgtt gggtccattg gtcaatgcca
      541 ttcaatagtg gttttttatt gacataattg taattaacaa tatcaccgtc ggctataaca
      601 atcattttat tttcaattcc tgaatctaac tcttttgtta gagtgaatgg tttaacacga
      661 tttttaaaag cggaattgaa tttaccttct aacaaaaccc caatagtttg atttcctttt
      721 gaaaagtctt cctcattcaa gttatccata aattgatata aattgacttc taatggagct
      781 ccaacagctt ttgattgagc agaacttttc agcaaaacag tttttttaat accattttta
      841 agggtgtcaa ttgtgtttgc aaattccaac ttcactaaat tgatgttttt agtaatggaa
      901 tgattgtctt ccgatttaat aatgggagaa tacaaccaat caatcggaac atctccttgt
      961 gcggattgag ctgttatagg ggtagaaagc aaatcctgaa ccaattggta attgatacgg
     1021 atgccgtatt taaaaaacaa atcatccaaa ttcaattctc ttggataagc caaagctgaa
     1081 ttgttctctg tttgaagtga atcaatgtcc atcacaacct tgtctatcaa ccacatactt
     1141 tttccgccat tcatcacata ttggtctaag atgtattttt ctttgtctga aaaagcttcc
     1201 gttggtttgg ctataatagc ggcatcaaat cgatttaaat tttctaaaat cttttgcgga
     1261 ttttcctgaa gtgagtcgag gttaaattct cctaattgat aatactcttt taagtttaac
     1321 aaaaaatcgg cttggtattt atctgctatt tctccatttc cttttaaaac agcaattttt
     1381 ttctgttttc ctgtggttat ttttgtgagg gcatcagcaa aagcaaattc taataattga
     1441 actgatttag ttatgttttc tgagggtgaa ttgccaaaat tattgactaa taaaggaaca
     1501 cgaactgact tttgaccttg gttggcaatt gcccaaggaa aaatttgtat cagttctttt
     1561 ttaccgtttt tattactgtt gatgttggtt ggtgtaaaac ctaaactgac caattcttga
     1621 acatattgtt ctgctttgct ttcgtcttca atcgggttat taaattgaaa aatcaggttg
     1681 gaattataac tgcggaattc ttctaagatt tgtttggttt cattttgaag acgtttaaac
     1741 tccggtggaa attgaccgtc taaaaaaaca tcaataaaaa tgggttcttt gatttctttt
     1801 attaattgaa tggtagtttc tgaaagagta tatcgtttgt cgtgtgttaa atcaaatcgt
     1861 ttgaaataaa aattcccaat gatgttgagt ataatcacta acatgaaaag caaaaatagc
     1921 tgttgcaggt ttttcttagt ttgagttttc attagttttt caaggattta agttgataaa
     1981 ctgttcctgc caaaaatagt acggtcacac tactgaaata taaaacatct cgagtgtcaa
     2041 tcacaccgcg actgatactt ttataatgat aatccattcc aattgcagaa accacgtctg
     2101 caaatgaagc agtaaaaccg gctattcctt caaatccaaa ataaaaaaag aaacataaaa
     2161 ataccgaaag aataaaggca acaatttgat tttcagacaa tgtagaggta aatattccga
     2221 tagctgtata agatgctacc aaaaacaata agcctaaata tgagccgatc gttgtgccaa
     2281 ggtctaaatt tccttccggc aaaccgtatt ttgaaacaac aaaaacataa accagcgtgg
     2341 gaactaaagc aaaaataatt aatagaaaag cacctaaaaa tttaccgttt acaatttgcc
     2401 agctcgttaa aggtttggtt aataataatt caatggttcc ttgttttttt tcatctgaaa
     2461 aactcctcat ggttactgcc ggaatcagaa aaattaaaat ccaaggagcc aaagtaaaaa
     2521 acggattcaa atcggcaaat ccactgtttg caatattaaa atcaccctca aaaacccaaa
     2581 ggaaaagccc attgagtaat aagaaaatgg caattactaa ataaccaatt ggtgaaccga
     2641 aaaaggtttt tatttctctg aaaagtattg ctttcattta ctttgttttt tnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttaaactaa agaaactaat ttattgacac
     2821 tccaagcttc cggtttagca ttaaaaagtt gcttagtaaa ctgccataca tcataaaata
     2881 attccgaatt tctgtaattt tctaaatcaa tttcggtttc ccaataactg taagtaaaaa
     2941 acaattccgg attattttta tcttgataca attctaacaa acgatttccg ggtgcatttc
     3001 gtattttttc tttcatcaat tcaaaatttt ccaaaaacgt tggaatatgt tcggtatgaa
     3061 aactcatttt tacgattcgc acaaacatgg taacttattt aaaattaata gtaatagtat
     3121 ctcgatagct aaagcctaac aaactagcgg cactacccac tgttttagga ttacttctaa
     3181 aaatagcaat ttctaagaag ccggcttcat tgaaaatagc taatttttct ccttcataat
     3241 atttaatggg aaatttatct gaaatggcta cagaagaata attgggcaaa atggttttta
     3301 tcgattttgg tttcattaca atttcatacg ttcggccttt actcaattcc aggaattgtt
     3361 ttttagaaat attgaatact atatttccaa aatgatcaat gtaaatcaca ttccctttga
     3421 tcgaatttcc atctttagaa actatcggtt gaaaatcagt aattgatttt aattcggtaa
     3481 tttcttttcc aattacgttc aaagttccgc cttttgctaa atgacaagca acagttttaa
     3541 acacatccat atcagttgca ttttccggta atcggtcgtg aatgttgatg gctacaatct
     3601 tttgtggaag gattcgttgc gttaacatac tcaaaatacc gttatcggca caaataaaga
     3661 attggtcatt ccattgcatg gcaagatgtt cattttctgt atttcgttcc gcatcaacac
     3721 ctattaaatg aacagttcct ttggggaaat ttgcgaagga agcctgaaga atataacttg
     3781 cttcagctat attaaagggg tctatttcat gcgatatgtc aatgatttta gcttcaggaa
     3841 attcagagat gattttacct ttcaaagcac caacaaaatg gtctttgagt ccgtaatccg
     3901 tcgttaaagt aattattgac atctttttgt ttataactat tcgtaaaacc tacgaacttt
     3961 tttgtttaaa tttgagtgat gcaaatctag taattaaaaa ctatttttta attttaaaac
     4021 aactgctttt gaacgaaaga atcatcgaat tacacgacat tgccccaaaa gagttttggg
     4081 gagctcaaga ttctcatctt gaaaccatca aaaagtacta tccgaagcta aaaattgtag
     4141 cccgaggcac caccttgaag gcttatggcg agaaggaagt attagatgag tttgaaaacc
     4201 gttttaatcg cctgatgctc cattttacac gttacaataa cattgatacc aatgtgattg
     4261 atcgtgtgat acaaagtaat tcggctgaag agcacaaaat gcatgaccat gacaaaattt
     4321 tagtacatgg tattggagga aaattaatca aagccatgac gccaaaccag cagttaatgg
     4381 ttgatttgat tcataaaaat gacatggttt ttgctgttgg tccggccgga acaggtaaaa
     4441 catataccgg cgtagcgtta gctgtaaaag ccttgaaaga aaaacaagtt aagcggatta
     4501 ttttaaccag acccgcagta gaggcagggg aaaatttagg ttttttgcct ggggatatga
     4561 aggaaaaact tgatccgtat atgcaaccgt tatacgatgc attgcgtgat atgttgccac
     4621 cacaaacatt ggaagattat attttaaaag gaattattca aattgctccc ttagctttta
     4681 tgcgtggaag aacattggac aatgcgtttg taatcttaga cgaagctcaa aatacgactc
     4741 attctcaaat gaaaatgttt ttaactagga tgggtaagaa cgcaaaattt attattaccg
     4801 gtgatcccgg tcaggtagat ttaccacgaa gaactatatc aggtttaaag gaagccatat
     4861 tgattttaaa agatgtggat ggtattggta ttttatactt agatgataaa gatatcgttc
     4921 gtcatcgatt agtcaaaaaa gtgattgatg cctataaaag tattgaaaat ttggattann
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca ccacaaaatg aacacaatta
     5101 caactactaa ttttcagttt cccaatcaaa aatcagttta tcacggtaaa gtacgtgaag
     5161 tgtacaatat caatgacgag attttagtca tgattgcaac tgaccgtctt tcagcttttg
     5221 acgtggttat gccaaaagga attccgtata aaggtcaaat tttaaatcaa attgcaacaa
     5281 aattcatgca actaaccgct gatattgttc ccaattggtt aattgcaacc cctgatccaa
     5341 atgtggcagt gggacattta tgtgaacctt tcaaagtaga aatggtgatt cgtggttatc
     5401 tatccggaca tgcagctcgt gaatatgcct tgggtaaaag aattctttgt ggagttcaaa
     5461 tgccggatgg tttaaaggaa aacgatcaat ttcctcccga aactacggga tcaattataa
     5521 caccaacaac taaagcagca aatggttcgc acgatgaaga tatttctcgg gaagctattt
     5581 tagaacaagg aattgtaagt gaagaagatt atttagtgtt agaaaaatac acccgtgctt
     5641 tataccaacg cggaacggaa atagctgctt caagaggatt gattttagta gatactaaat
     5701 atgaatttgg aaaaacaaaa gaggggcaaa tagtattgat tgacgaaatt cataccccgg
     5761 attcttcccg atatttttac ttagaaggtt atcaggaaag acaaaaaagc aacgaagaac
     5821 aaaaacaatt atccaaagag tttgtacgtc gctggttaat tgaaaatgga tttcaaggaa
     5881 aagaagggca acaaattccg aatatgtctg aggcttatat cgaaacggtt tctgagcgat
     5941 atatagagtt gtatgaaaat atcttaggag aaaaattcgt gaaagcagat atttctaaca
     6001 ttcaggaacg tattgaaaaa aatgtactgg cctatttaac taaataaaaa attagttctc
     6061 aaaatctaaa ccacgttttt acgtggtttt tttatttcct aatgttaaaa ttatgttata
     6121 gattcctgat tttgtaacat ttagattttg ttatcgtcta ctctaatata tcaatcaatc
     6181 aatcaatcaa tcaatcaatc aaaaacgaac agtatgaaaa aatcagttat ttatttagga
     6241 attgcccttt taggttttgg aacatttgca aatgctttca atggttctga aaaaacgcca
     6301 acaaaagaag ttggaattta tgcaaaatat tatgctactc ctttatgtgt tgccatcagt
     6361 aagggtgaag tggaagtcgt aagaaaattt attgagtatg gtgccaatgt aaatgagaaa
     6421 acaaatgggt taactccgtt gatgtatgcc gcccgttaca atcaggtgga gattattaaa
     6481 attttactgg aaaaaggggc tgatgtaaaa gtgaaagatg acaaaggttt tacagcactt
     6541 gaccatgcgg aaaattcaaa agcaacggaa gcggttgctg ttttaaaaaa ttatttgaat
     6601 tcataattaa aattggttag ttaaaaaaaa tcccatccgt tttttggatg ggattttttg
     6661 ttattttaaa atggtttgag tcctgtcagg tcctacggaa acaactttaa taggtacttc
     6721 aatttctttt tcaatgaatt cgatatatgt ttttagctct gccggaagtt cgtcataact
     6781 agtcattccg gttaagtctg cttgccatcc tttcatttct gtgtaaacag gttcaacatt
     6841 ttccggttcg atgttataag ggaaatgtgt gattttttga cctttgtatt gataagccgt
     6901 gcatactttc aaggttttaa atccggaaag aacatcacct ttcatcatgt ataaaccggt
     6961 tactccattt acttgaactg cgtattttaa ggcaactaaa tctaaccaac cgcaacgtct
     7021 ttttcttcct gttactgagc cgaactcatt tcctactttt gccatgacat caccggcttc
     7081 ctcaaaatct tctgttggaa acggaccgct gcctacgcga gtggtatagg ctttgaatat
     7141 accaaataca ttttttactt tattgggagc aatgcctaat cctgtgcaag ctcctgcggc
     7201 tgtggtgttt gaagaggtga caaaaggata tgttccgaaa tctacgtcta ataaagatcc
     7261 ttgagcacct tcacaaagaa tggttttgcc ggattttaaa gcctgattta aatattcttc
     7321 actatcaatg aattgtaatg atttcaacac ttcgatggaa gcgaaaaact cttcttccat
     7381 ttcgggtaga ttgtattgta ggtttacatc ataaaaggaa atcatagctt catgcttgtc
     7441 tgctaaggct ctgtatcgtt ctttgaaatc ggccatttcg atatcgccga cacggattcc
     7501 gttacggccg gttttatcca tataagttgg accgatgcct tttaaggttg aaccgatttt
     7561 ggcttttcct ttagaggctt cagaggctgc atctaataaa cggtgggtag gtagaattaa
     7621 atgggctttt ctggaaatca atagtttaga tttgatatcc atgttgaatt tttctaatcc
     7681 ttccaattct ttttgaaata cgaccgggtc tatcacgact ccgttgccga taatattcat
     7741 ggcgttttta tgaaatattc cggagggaat agttctgagt acatgtttta ttccgtcaaa
     7801 ttctaaggtg tgtcctgcat tcggacctcc ttgaaagcga gcgataatgt cgtatttaga
     7861 agtgagtacg tcaacgattt ttccttttcc ttcatctccc cattgcaggc cgagtagtaa
     7921 atctacagtc attttttttg taagttgtta tagttaggtt gtagttattt attcttttat
     7981 tgctttttta ttaccgtata gataaaggga atgattgtgt atttcaattc cgaagatgtc
     8041 ttcaatggtt tgtttgattt gttggattct tgggtcgcaa aattcaatta cttcaccagt
     8101 gtcggtcatg atgatgtgat cgtgttgttt atcaaagtat gacttttcgt agtgagcttg
     8161 gttttgtcca aattgatgtc tgcgaaccaa gccacattct aataacaatt caattgtgtt
     8221 gtataaagtg gcacgactaa cgcggtagtt tttgtttttc attttgatat acaacgattc
     8281 gatgtcaaaa tgttctttgt tgttgtatat ttcttgaagg attgcataac gttcgggggt
     8341 ttttcgatgc cctttttgtt ccaaatactt ggtaaaaacg ttttttacta cttcttggtt
     8401 ttttatcgtt tccattgtta ttaatatcta caaagataca ctatttttat attttgactg
     8461 tttttggcaa tcaagtttcg gttgagatta ttaacaaata gctgtgaata attactctaa
     8521 tttggcgttg tttacccttg ttactttgtc gattccgtca atttttttga tgttttcaat
     8581 caggcgtttt aagatggtgt tgttttgcac gacaacggtg acctgaccat tgaatattcc
     8641 ggcgtcacca ctaagggaga tgctttgaat gttgacattc atctggtttg agattaccct
     8701 ggtgagttca ttggttaaac caaggctgtc catgccggtg attcttaata cggctttgaa
     8761 atcttcttga ctggagtcta tccaacgggc ttgaatgatg cgataggcat aattggattg
     8821 tagactaatg gcattgggac aatctttttt gtgaatcttg atgccgtcat tgatggtgag
     8881 gaaaccgaaa acatcatcgc cgggaatggg gttgcagcaa ctggagagtt tgtagtcgag
     8941 tttgtcttgt tcggctccaa aaactaacat atcgaagttt ttgcttagct cattcttttg
     9001 tatttgttca ggagcgacgg atggtgatcg ttttatcttc ttgaagaagt tgattagcgt
     9061 gttgcttttt tgtgcggcgt aatcttttag ttgttgattg tcgatggttc ccagaccgat
     9121 tcgatagaag aggtctaaac tggttttgag tttaaaataa ctaaccaact catttgttgt
     9181 tgtttcgttc aacgtgattt tgaggtggcg tagtttccgg ttaaggagtt cttttccgtc
     9241 ttcagcgatt ttcttggtgt cttcgttgag gacgtttttg attttattct ttgctcttga
     9301 cgtggtaacg taatctaacc agtgtgaatt tggtttttga ttgggcgatg tgatgatttc
     9361 tatttggtca ccgctgctta acacatggtt taggggtact aattttccgt ttacgcgggt
     9421 gccacgggtt cttacgccta tttccgagtg gatgctgaag gcaaagtcta gcgaagtggc
     9481 tcctttgggc aatgatttaa tttcaccttt tggggtgaaa acatagattt ctttggaata
     9541 gagattcaat ttgaagtctt ccacaaagtc aactgcgttg gaggtggagt tttcgagggc
     9601 ttcttttagt aagttcaacc aagtgtcgag ggtgttctct tcagagattc cttgtttgta
     9661 tttatagtgt gcggcatatc ctttttcggc gatttcgtcc atgcgttcgg agcggacttg
     9721 tatttctacc caacggcctt ttgggcccat gacggtgatg tgaagggctt cgtaaccggt
     9781 tgatttggga gacgaaatcc aatcgcgtaa tcggctgggg ctggggcgat aatggtcggt
     9841 gacgatggaa tatattttcc aagcgacgaa tttttcgtcg tgttggtttg atttataaac
     9901 aattctgagg gcaaatttgt catatacttc gtcgaaagag acaccttgtg ccagcatttt
     9961 gcgacggatg gagtagattg actttggacg gcctttgatg gtgtattcta tgccttcatc
    10021 gtttaatgat tttttaagca cgtcggaaat ggaggtgata taggcttcct gttcctcttt
    10081 gctttctttc attttgctca cgatgtcggc atatacgtcc ggttcggtgt atttgaggcc
    10141 taaatcttcg agctttgatt tgatattata caaccctaat cgatgggcga ggggtgcata
    10201 aatgtataac gtttccgaag cgattttggc ttgtttataa tccaccatcg actccatggt
    10261 ttgcatgtta tggaggcggt ctgcgagttt aatgagtatt acccgaacgt catcgttcag
    10321 ggtgagcagc attttgcgga agttttccgc ttgcatggat atttcttggt ctgtttttac
    10381 tttcgcaatt ttggtaaggc cgtctacaag ttgtgcaatt ttagggttga acattttttc
    10441 gatttcgtgg acggtgatgg gggagtcctc cactacatcg tgcatgagtg ctgcggcgat
    10501 tgctgtggct ccaagtccga tttcggcggc gacaatttta gccacggcaa tggggtggaa
    10561 gatataggct tctccggatt tgcggcgttg atctttatgg gcatcgacgg caacgtcaaa
    10621 cgcttttcgg atgatttttt tgtcttccgg ggtgagggtt tggtagctta tgcggagtaa
    10681 ctctttgtat tccttagcga tggctttgtt ttcctgttct atttcaagtt ctgtcatggc
    10741 gttttgttca cttactaaaa gtagga
//
LOCUS       DAOTCW010000076        10330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_4232_length_10330_cov_39.901217, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000076 DAOTCW010000000
VERSION     DAOTCW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10330
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4232_length_10330_cov_39.901217"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /gene="katG"
                     /locus_tag="P5335_07400"
     CDS             complement(<1..1523)
                     /gene="katG"
                     /locus_tag="P5335_07400"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HRZ74738.1"
                     /translation="MNNSQPGKCPFGHGANTEVSNSVMEWWPKSLNLDILHQHDTKTN
                     PYGESFSYAEAFKKLDLEALKNDLKALMTNSQDWWPADWGHYGGLMIRLAWHAAGTYR
                     VADGRGGSNTGNQRFAPLNSWPDNGNLDKARRLLWPIKKKYGNSISWADLFILAGNMA
                     YESMGFKTFGFAGGREDIWHPEKDVYWGAEKEWLAKTGSNGTRYSGERDLENPLAAVM
                     MGLIYVNPEGVDGNPDPLKTAHDMRITFKRMAMNDEETVALTAGGHTVGKAHGNGDAS
                     ILGESPEGAEIENQGFGWMNPKGNGNAGDTVTSGLEGAWTTNPTTWDNGYFEMLFNHE
                     WELRKSPAGAWQWEPINIREEDRPIDAHNPNARQNPIMTDADMALKVDPQYRKISERF
                     KNDPAYFNDVFARAWFKLTHRDLGPKNRYLGADAPTEDLIWQDPIPMVDYTLSENEIQ
                     ELKEKLLNSGLTQAELINTAWDSARTFRGSDFRGGANGARIRLAPQKDWEGNEPERL"
     gene            1804..2028
                     /locus_tag="P5335_07405"
     CDS             1804..2028
                     /locus_tag="P5335_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74739.1"
                     /translation="MNTSKIIGAVLIIISLGIGYIGSTKIADSSKSVNLLGLKIDASN
                     ESGKQEGYLYLGLAIVLFAGGIYTLTKLKK"
     gene            2081..2764
                     /locus_tag="P5335_07410"
     CDS             2081..2764
                     /locus_tag="P5335_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRZ74740.1"
                     /translation="MSNTIASLKNVNISQENNMILKNINLDVKRGDFIYVIGKTGSGK
                     SSFMKTLYADLPLTNGEGFIVDFDLAKLKENDIPYLRRKIGIVFQDFKLLPDRSVFDN
                     LEFVLKATGWKETAEMKQKIDEVMEKVGMKSMLHKMPHQLSGGEQQRVAIARALLNDP
                     EIILADEPTGNLDPQTSVEVMEVLRKINQNGKTIIMATHDYALIMKFPSKTLKCEDST
                     IFEVVQRTV"
     gene            complement(2733..3980)
                     /locus_tag="P5335_07415"
     CDS             complement(2733..3980)
                     /locus_tag="P5335_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74741.1"
                     /translation="MTLFIQKYFYHFILVAIGLLWLSVLMGLLDISAQTLIYPDAGDY
                     NLSATNLYWYFRGHCYRPMLMAAIYGFPLLFGASDFDLYGIAIYINVFLWLATILILF
                     ELLQTYVSKKIAFLISLCFVFFVSNALFNFHLLAETPFLFFLISGFYFIQKYTQTKVF
                     KWLVIALSIIVLSMLIKPASKFFAILMLLYFAKILFSQYKQKVMVFLYASLVLCFVQA
                     AGLKHQFGDFTISYIDSVTFYNYLFSKADCYRKGVEYNQIKNPRAEYLFTHKAHEQKQ
                     IANADIKEQLLNNKWNLIKAYGNNFIWNSVTGSICINGYSNVKGNPDFETNQTILSWI
                     SKYQNRLMTGLGIVLGLLSVWQYYRKDTFLLFAGLVILYIIGISGISSDQGDRFHLIT
                     YPFTLLLLAKYVSTKPFFAPLQK"
     gene            4074..4808
                     /locus_tag="P5335_07420"
     CDS             4074..4808
                     /locus_tag="P5335_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRZ74742.1"
                     /translation="MENQSNPKKLSIIIPAYNEGRTIHLILNKIKDVVLPEAVLKEII
                     IVNDCSTDETEATLLAYQFNNPEFVVHYYKHDVNKGKGAAIHTGISKATGDFILIQDA
                     DLEYDPEEYTVLLKPMLGGHADVVYGSRFMGGKPHRILFFWHTIGNRFLTFLSNMFSN
                     LNLTDMETCYKLFNATLLKSLSLHENRFGFEPEVTQKIARIPKIRIYEVGISYYGRTY
                     DEGKKINWKDGFWAIWCIFKYGLLRL"
     gene            4810..5688
                     /locus_tag="P5335_07425"
     CDS             4810..5688
                     /locus_tag="P5335_07425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRZ74743.1"
                     /translation="MLSILIPTYNYAILALVKNVHQQCLEAQIPFEIICLDDQSDNDY
                     QIENLNISTLPNCIYNVNQTNVGRTRTRQRLAEQAKYDWLLFLDADVIPVTSAFIQHY
                     IDAIQLNTVIFGGYQYEKESTDATKILRFRYGKEREEKYASERNKNPYTYVFSGNMLV
                     PKSIFLNFNYQANEKNYGMDIYFGYQLYQQQISVLHLDNPILHLGLETNEVYFQKALQ
                     GVESRKKYLLHEKGVDQMNPLVQAYAKLKKLGLTKLVAVGFKIVAPFLKKNILSTNPK
                     LVYFDIYRLGYLCSLE"
     gene            5699..6661
                     /locus_tag="P5335_07430"
     CDS             5699..6661
                     /locus_tag="P5335_07430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRZ74744.1"
                     /translation="MKPIFSIVIPLFNKEKEIQQTLESVLKQTFHDFEVIIINDGSTD
                     KSEQIVNSFTDERILLISTDNKGVSQARNFGINYANGEIIAFIDADDYWFPNHLEKLF
                     ELHQKFPETGLLATNYEFYFSEHNIIRPTFDGIPAENWSGIVKDFFKSSMTYRLAWTS
                     AVAVPKAILDKIGCFDETITLGAGEDTDLWIRIAVDYPVAFSTEISARYNMTAQNKIS
                     FSDTTKRNFARLDKFYQQEKENPSLKKFLDLYRVVYALKHKLADDEKNFAFYYSAIAM
                     ENCPLKSRILLKLPTFILKKLFVFGRLLNSNKMYFNFYTRLFKS"
     gene            complement(6647..7636)
                     /locus_tag="P5335_07435"
     CDS             complement(6647..7636)
                     /locus_tag="P5335_07435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRZ74745.1"
                     /translation="MTKETSFVSVGDFLDVYYKVKQKGFLQLASLFNLTKNKRVSAKW
                     NQYQSSSDFWIIPSLQQYWNNLISGNPEVSYESYVVQKYFHNKTNLSLLSIGCGEGKH
                     ERKFAVSGLFSKVIGVDISNERIERAKQTALENNLNIEYIAGDFHQMNFNNESFDLIL
                     FDSSLHHFNDIKGFLKNEILPLLKSEGILVANEFCGPNRLQWKKEQLDFANDLLKKLP
                     KKYKTLIDGKSVKRKVYRPGLIRMMLVDPSEAPDSANLKNALQNNFTVLEEKKLGWNI
                     LHILLKGIAHNFLKNDDETNQLLAYLIKKEEEFVERVGETDSIFGVYQKSRLE"
     gene            complement(7642..8031)
                     /locus_tag="P5335_07440"
     CDS             complement(7642..8031)
                     /locus_tag="P5335_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HRZ74746.1"
                     /translation="MSFQLIAIPKIEDPRGNLSVIEKNVLPFEMKRVYYLYDVPSGAE
                     RGGHSHIQQKEFLVALSGSFDVILNDGKTEKIITLNKPFEGLLINEGIWRELKNFSSG
                     SVCLVVASDVFEEEDYIRDFIDFCNSK"
     gene            complement(8089..9216)
                     /locus_tag="P5335_07445"
     CDS             complement(8089..9216)
                     /locus_tag="P5335_07445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRZ74747.1"
                     /translation="MNRLYLYEFKQKRLQDKKIKIGLIGTSLAHGGAGKILALYSIGF
                     EKAGFEVHTILVNSTIDFEYKGNLVDLSKHKKGFFNRLNRFLILKKYIQKNQLDYLID
                     FRVRNKPLMEWLLNQFVFNCRYIPTVHSFELDFYFPKNDFLAKLIYRKAYKIVTVSKA
                     IQHKIEQKYSLKNVVAISNPLDFDEIETKSVQPILMTEKFILAVGRMEEDNIKQFDKL
                     LESYADSELPAQNIHLVFLGDGMFKHKLESIVAKANLASFVHFKGFQKNPYAFMKQAQ
                     FLVLSSRNEGYPNVLIEALACGTPVISFDCESGPNEIIQQEQNGLLVENQNIEKLTIA
                     MNRFVLEPELLKFCKANTLHSLEKHRFEQVISQWKETIFKK"
     gene            9215..>10330
                     /locus_tag="P5335_07450"
     CDS             9215..>10330
                     /locus_tag="P5335_07450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRZ74748.1"
                     /translation="MRILLIGEYSRLHNSLKEGLLTLGHEVTLVSDGDDFKNYPNDYS
                     YDAKWSKSKLLFYPRRALHKFFKIDIASIERGIRFYFLLPKLKKFEVIQFINEASINT
                     IASFEIYLIQKLLLHNNKLFLLSSGADAVNVQYMLDKKFKYSILTPYLEEQKASNEYP
                     YILRYVSKSHLKLHDYLYQNSKGVIATDFDYAIPLEGNAKFLGLIPNPINCAKIDFIP
                     MEIKDKVVLFLGINRHNYVKKGIHFFEEALKIIDEKYAAKVEIIITENIPYEKYIQLY
                     NKAHILLDQVYAYDQGYNALEAMAKGKVVFTGAEQAFVDYYQLTDRVAINALPDVHEL
                     VKELSFLIENPQEIIKIGKNARAFVEKEHDNVKIAEKY"
BASE COUNT         3423 a         1814 c         1743 g         3350 t
ORIGIN      
        1 tgtaaacgtt cgggttcgtt accttcccag tctttttgag gagctaatcg aatgcgagcc
       61 ccgtttgcac cacctctaaa atctgaacct ctaaaagttc gggcactgtc ccaagcggta
      121 ttgattaatt ctgcttgcgt taaaccactg tttagtaatt tctcttttaa ttcttgaatt
      181 tcattttcag ataaggtgta atcaaccatt ggaattgggt cttgccaaat caaatcttct
      241 gttggagcat ctgcaccaag gtatctgttt ttcggaccta aatcacggtg ggttaatttg
      301 aaccaagctc tggcaaaaac gtcattgaaa taagcagggt catttttgaa tcgttcagag
      361 atttttctgt attgcgggtc tacttttaaa gccatgtctg catccgtcat aattgggttt
      421 tgacgagcgt tagggttatg agcatcaatc ggtctgtctt cttctctgat attgattggt
      481 tcccattgcc aagcaccggc agggcttttt cttaattccc attcatggtt aaaaagcatt
      541 tcaaaataac cattatccca tgtagttggg ttggttgtcc aagcaccctc taaaccactg
      601 gttacggtat cacctgcgtt tccgtttcct tttggattca tccaaccaaa accttggttt
      661 tcaatttcgg caccttccgg actttcacct aaaatggaag catcgccatt accgtgtgct
      721 tttccaacgg tgtgaccacc ggcagttaaa gcaaccgttt cttcatcatt catggccatt
      781 cgtttgaaag taatacgcat gtcatgagct gtttttaaag ggtcgggatt tccgtcaact
      841 ccttccgggt ttacatagat taatcccatc atcaccgctg ctaatggatt ttccaggtcg
      901 cgttcgccgg aataacgagt tccattactt cctgttttag ccaaccattc tttttctgca
      961 ccccaataca catctttttc cggatgccaa atatcttcac ggccaccggc aaaaccgaat
     1021 gttttgaatc ccatcgattc ataagccatg ttaccggcta aaatgaataa atcagcccat
     1081 gaaatgctat taccatattt tttcttgata ggccataata atcttctggc tttgtctaag
     1141 tttccattat caggccaact gtttagtgga gcaaaacgtt gatttccggt gttgcttcct
     1201 cctctcccgt cagcaactcg ataggttccg gcggcatgcc aagctaaacg aatcattaat
     1261 ccgccataat gaccccaatc tgcaggccac caatcttggc tgttagtcat taatgctttt
     1321 aaatcatttt ttaaagcttc taaatccaat tttttgaagg cttctgcgta gctgaagctt
     1381 tcaccatacg gattggtttt tgtgtcgtgt tggtgaagta tgtccaagtt tagtgatttt
     1441 ggccaccact ccattactga attgcttact tctgtattgg ctccgtgacc aaacggacat
     1501 tttccaggtt gtgaattgtt catatttatt attttaagtt taaattgatt tgttttaaaa
     1561 agtatgccat aaaaactact ataaaaccac catttaattc cttaaatagt agttttaatc
     1621 taatgattta ataatctcaa atttaacaaa ataaactcgt ataattattc tttttcaata
     1681 gataaaaact attgttttat ataaatccga taataattat acagctctat ttttaataaa
     1741 cgagagaaat gctgattcta aacttaaatt cattatcatg tagaaatact aaattttaaa
     1801 aaaatgaata catcaaaaat tataggagcg gttttaatca ttattagctt agggattggc
     1861 tatattggtt caacaaagat tgcagacagt tcgaaatcag tcaatttatt gggtttaaaa
     1921 attgatgctt ctaacgaatc tggcaaacaa gaaggctatt tatatttagg ccttgcaata
     1981 gttctttttg caggaggaat ttataccctc actaaattga aaaaataatt ttgaaaacca
     2041 ttggtaactt actactttta ccaatcaaaa tagtaaattt atgtcaaaca ccatcgcatc
     2101 gttaaaaaac gtcaacattt cgcaagaaaa taacatgatt ttgaaaaaca tcaatctaga
     2161 tgtgaaacgg ggtgatttta tttatgtgat tggaaaaacc ggatccggga aaagtagttt
     2221 tatgaaaaca ctttatgccg atttaccatt aaccaacggc gaaggtttta ttgttgattt
     2281 cgatttggct aaacttaaag aaaatgacat tccgtatttg agaagaaaaa taggtatcgt
     2341 ttttcaggat ttcaaattac tgccggaccg aagtgttttt gataacttag aatttgtatt
     2401 aaaagcaacc ggctggaaag aaacagctga aatgaaacaa aaaattgatg aagtgatgga
     2461 aaaagtaggc atgaaatcaa tgcttcacaa aatgcctcat caactatcgg gtggagaaca
     2521 acaacgggtg gccattgcca gagccttatt aaacgatccg gaaataattc tagcagatga
     2581 acctaccgga aatctagacc cacaaaccag tgtggaagtc atggaagtat tgcgaaaaat
     2641 caatcaaaac ggcaaaacga ttatcatggc aacacatgat tatgcgttga ttatgaaatt
     2701 cccatctaaa acgttgaaat gcgaagacag cactattttt gaagtggtgc aaagaacggt
     2761 ttagtactca cgtattttgc taacagcaat aaggtaaacg gataagttat caggtggaat
     2821 cgatcgcctt gatcacttga tatacctgat attccaatga tataaagtat cacaagccct
     2881 gcgaaaagca aaaaggtatc ttttctgtaa tactgccaaa cggacaacaa acccaacacg
     2941 attcctaaac ccgtcattaa acggttttgg tatttcgaaa tccaagataa aatagtttgg
     3001 ttagtttcaa aatcgggatt tccctttacg tttgaatacc cattgatgca aatggaaccg
     3061 gtaacagaat tccaaataaa attgttgcca tacgctttta tcaaattcca tttgttgttc
     3121 aacaattgct ctttgatatc tgcattcgca atttgttttt gctcatgggc tttgtgggtg
     3181 aataaatatt ccgctctggg attcttgatt tgattgtact ctactccttt tctataacaa
     3241 tctgcttttg aaaacaggta attataaaac gtcacactat caatataact aatcgtaaaa
     3301 tcgccaaact gatgttttaa accagccgct tgaacaaaac ataaaaccaa agaagcgtac
     3361 aaaaaaacca ttactttttg tttatactgc gaaaaaagta ttttggcaaa gtagagtaac
     3421 ataagtattg caaaaaactt actggcaggc ttgattagca tgcttaaaac gataatggaa
     3481 agtgcaataa caagccattt aaaaaccttt gtttgagtat acttttgaat aaaataaaac
     3541 cctgaaatca ggaaaaaaag aaaaggggtt tctgctaata aatgaaaatt aaacaatgca
     3601 ttgctaacaa aaaaaacaaa acaaagtgat atcaaaaatg cgattttctt acttacataa
     3661 gtttgaagta attcaaataa aattaaaata gtagctaacc ataaaaaaac attaatataa
     3721 attgcgatgc catacaaatc aaaatctgaa gcaccaaaca acaatggaaa accatatata
     3781 gccgccatga gcattggccg ataacaatgg ccgcgaaagt accaatataa attagtagct
     3841 gacaagttat aatctcccgc atcaggataa atcaaggttt gagcagaaat atccaataat
     3901 cccattaaaa cagatagcca aagcaaccca atagccacga gtataaaatg ataaaaatac
     3961 ttttgaataa aaagtgtcat atatttgaat aaaataagtt cactatttgt tgaaaaaagc
     4021 taaattgctc attcaaaaat aatagtcaaa aatagggtat tattttcaat tgtatggaaa
     4081 accaaagcaa tcccaaaaaa ttatcgatta tcattccggc ctacaatgaa ggaaggacaa
     4141 ttcatttgat tttaaacaaa attaaggacg ttgtgttgcc tgaggctgtt ttgaaagaaa
     4201 tcattatagt gaatgactgc tcaaccgatg aaaccgaagc aacactatta gcctatcaat
     4261 ttaataatcc ggaatttgta gttcattatt acaagcatga cgtaaacaaa ggcaaaggtg
     4321 ccgcaattca caccggaatt tcaaaagcta caggtgattt catactcatt caggatgctg
     4381 atttagaata tgacccggag gaatatactg ttttattaaa accaatgcta ggcggacatg
     4441 ctgacgtggt ttatggttcg aggtttatgg gcggaaaacc gcaccggata ttgtttttct
     4501 ggcataccat tggcaatcgg tttttaactt tcctatcgaa tatgttcagc aatttaaact
     4561 tgaccgacat ggaaacgtgt tacaaattgt ttaatgccac ccttctaaaa tcactttctt
     4621 tacatgaaaa tcgttttggg tttgaaccgg aagtcactca aaaaatagct cgcattccca
     4681 aaattcgaat ttatgaagta ggcatttcct attacggaag aacgtatgat gaaggaaaaa
     4741 aaatcaattg gaaagatggt ttttgggcaa tttggtgtat ttttaaatat ggattattaa
     4801 gactttaata tgctatccat tttaattccg acgtataatt atgctattct tgcacttgtt
     4861 aaaaatgtgc atcaacaatg cttagaagca caaattcctt ttgaaatcat ttgtttggat
     4921 gaccaatctg ataatgatta tcaaattgaa aatctaaata tttcaacttt acccaactgt
     4981 atttataacg ttaaccaaac caatgtcgga cggacgcgaa cacggcaacg attggcagaa
     5041 caagcaaaat atgactggtt attattttta gatgcagatg tgattcccgt tacttccgct
     5101 tttatccaac attatatcga tgctatacaa ctgaacacag ttatttttgg tggctatcaa
     5161 tatgaaaagg aatcaactga tgctacaaaa attttaaggt ttaggtatgg aaaagagcga
     5221 gaagaaaaat atgcttctga acgaaataaa aatccctata cctacgtgtt ttcaggaaat
     5281 atgctagttc caaaatcaat ttttttaaac tttaattacc aagccaacga gaaaaattat
     5341 ggcatggata tttattttgg atatcaactt tatcaacaac aaatttctgt gcttcatctt
     5401 gataacccaa tccttcattt aggattagaa acaaacgaag tttattttca aaaagcgttg
     5461 caaggagttg aatcccgaaa aaaatacctt cttcatgaaa aaggtgtgga ccaaatgaat
     5521 ccgttagtgc aagcttatgc caaactgaaa aagctgggat taacaaaatt ggtagcagtt
     5581 ggttttaaaa ttgtagctcc ctttttaaag aaaaatatat tgagtacaaa tccaaaatta
     5641 gtttattttg atatctatcg cttaggttat ctttgctcat tagaataatg caatagtcat
     5701 gaaacccata ttttctatag ttattccgct tttcaataag gaaaaagaaa ttcaacaaac
     5761 gcttgaaagt gtgttgaaac agacttttca tgattttgaa gtgattatca tcaatgacgg
     5821 gtcaactgat aaaagtgaac aaattgtaaa ttcgtttacc gatgaacgaa ttttgctgat
     5881 ttctactgat aacaaaggtg tttcacaagc gagaaacttc ggaattaact atgctaacgg
     5941 ggaaattatt gcctttatag atgctgatga ttattggttt ccaaaccatt tagaaaagtt
     6001 atttgaattg catcaaaaat ttccggaaac cggattactg gccactaatt atgaatttta
     6061 tttttccgag cacaatatca tccggccaac ttttgatggc attccggcag aaaattggag
     6121 cggaatagta aaagactttt ttaaatcaag tatgacctat cgattggcat ggacgagtgc
     6181 tgttgccgtt ccgaaagcta ttttagataa aattggttgt tttgatgaaa caataaccct
     6241 aggtgcagga gaagatactg atttatggat tcgaattgct gttgattatc cggtggcttt
     6301 ttctactgaa atttctgccc gatataatat gacggctcaa aacaaaataa gtttcagtga
     6361 tacaacgaaa cgaaactttg cccgattaga taagttttat caacaagaaa aagaaaatcc
     6421 ttcccttaag aaatttttag atttgtaccg ggttgtttat gcactcaaac ataaattagc
     6481 cgatgatgaa aagaactttg ctttttatta ttccgccatt gcgatggaaa attgcccttt
     6541 gaaaagtaga atattgttga agttacctac ctttattttg aaaaagctat ttgtgtttgg
     6601 acggttgttg aactcaaaca aaatgtattt caatttttac acccgcttat tcaagtcttg
     6661 atttttggta aacgccaaaa atactgtcgg tttcacccac tctttccaca aactcttctt
     6721 cctttttaat caaataagca agtaattgat ttgtttcatc gtcatttttt aagaaattat
     6781 gagcgatgcc ttttaaaaga atatgcagaa tattccatcc taattttttt tcttccaaca
     6841 cagtaaaatt gttctgcaaa gcatttttta aattagcaga atcaggagct tcggaaggat
     6901 caaccaacat catccggatt aatcccggtc gataaacttt ccgcttaacg gatttgccat
     6961 cgatgagcgt tttatatttt ttggggagtt tttttagtaa atcattcgca aaatcaagtt
     7021 gctctttttt ccattgcaaa cgattggggc cacagaattc attagctact aaaattccct
     7081 ctgattttaa taatggtaaa atttcatttt ttaaaaaccc tttgatgtca ttaaaatgat
     7141 gcaaactgga atcgaataaa attaaatcaa acgattcatt attaaaattc atttgatgaa
     7201 aatcgccggc tatatactcg atattcaggt tgttttccaa ggcagtttgt ttggctcttt
     7261 ctattctttc gttagaaata tctacaccga ttacttttga aaatagtccg gaaacggcaa
     7321 atttacgttc gtgttttcct tcgccgcaac caatggataa taaggataaa ttggttttgt
     7381 tgtgaaagta tttttgaaca acatacgact cataactcac ttccggattg cctgaaatta
     7441 agttattcca atactgttgt aaagaaggaa taatccaaaa atcagaggaa gattgatact
     7501 ggttccactt tgctgaaacc cgtttgtttt tagttaaatt aaaaagggaa gccaattgca
     7561 gaaatccttt ttgtttcact ttatagtaaa cgtcaaggaa gtcacccaca gaaacgaatg
     7621 aggtttcttt cgtcatttcg gttatttaga attacaaaaa tcgatgaaat cccggatgta
     7681 atcttcctct tcaaacacgt ctgaagccac cactaaacaa acggaacccg aagaaaagtt
     7741 tttcagttcc cgccatattc cttcattaat caataagcct tcaaaaggtt tgtttaaagt
     7801 aattatcttt tctgtttttc catcgtttag aattacatca aaacttccgc taagagccac
     7861 caaaaactct ttttgttgga tatgcgaatg tccgccacgc tcagcaccac ttggcacatc
     7921 atataaatag taaacacgtt tcatttcaaa aggcaaaacg tttttttcta tgacagataa
     7981 attgccacgc ggatcttcaa tttttggtat agctataagt tgaaaagaca aaatagttta
     8041 tttttaattt tgatttacaa atctaaggga ttaagtaatt agagtgcctt attttttaaa
     8101 aattgtttcc ttccattgtg aaatgacttg ttcaaatcga tgtttttcta atgaatgcaa
     8161 ggtgtttgct ttacaaaatt tcaataattc cggttccaaa acaaatcgat tcattgcaat
     8221 cgtcagtttt tcaatgtttt gattttccac taataagccg ttttgttcct gctgaatgat
     8281 ttcgtttggc cctgattcac aatcaaacga aattaccggc gttccacaag ccaaggcttc
     8341 aatcaataca ttaggataac cttcatttcg gctggaaagc actaaaaatt gagcttgctt
     8401 catgaaggca tacggatttt tttgaaaccc tttgaaatga acaaacgagg caaggttagc
     8461 ttttgctaca atcgattcca atttatgttt aaacatgcca tcacccagaa aaacgagatg
     8521 aatattttgt gccggtaatt cagaatcagc ataactttcc agtaacttat caaattgctt
     8581 gatgttgtct tcctccattc tgcccactgc taaaatgaac ttttcagtca ttaaaatagg
     8641 ttgcacactt tttgtttcta tttcatcaaa atctaatgga ttggaaatcg cgacgacgtt
     8701 tttgagcgaa tacttttgtt caattttatg ttgaatagct ttagaaaccg taacaatttt
     8761 ataggctttc cgataaatca gtttcgccaa aaaatcattt ttaggaaagt aaaaatctaa
     8821 ctcaaaacta tgcaccgttg gaatatatcg acaattgaaa acaaattgat tgagtaacca
     8881 ttccatcagc ggtttattgc gaacccgaaa atctattaaa taatctaatt gattcttttg
     8941 aatgtatttt tttaaaatca agaatcgatt caatcggttg aagaaaccct ttttatgctt
     9001 gcttaagtct actaaatttc ctttgtattc aaaatcaata gttgaattaa ccaggatagt
     9061 atgcacttca aaccctgcct tttcgaagcc aatggaatac aaagcaagta tttttcctgc
     9121 tccgccatga gctaagctgg ttcctatcaa tccaatttta attttcttat cttgcaatcg
     9181 tttttgttta aattcataca aatataatcg attcatgcga atcttattga taggcgaata
     9241 cagccgtttg cataattctt taaaagaggg acttttgaca cttggtcatg aagttacttt
     9301 agtgagtgat ggtgatgatt ttaaaaatta tcccaacgat tattcgtatg atgcgaaatg
     9361 gagtaaatcg aaacttttgt tctatccgcg aagagctttg cacaaattct ttaagattga
     9421 tattgcttct attgaacgtg gtattcggtt ttatttttta cttcctaaac tgaaaaagtt
     9481 tgaggtgatt cagttcatta acgaagcatc tataaatacc attgcatcct ttgagattta
     9541 tttgattcaa aaactgcttt tgcataataa caaattgttc ttactctcct caggtgccga
     9601 tgccgtgaat gtgcaatata tgctagacaa aaagtttaag tactctatac ttactcccta
     9661 tctggaagaa caaaaagcaa gtaatgaata tccttatata ctgcggtatg tttctaaaag
     9721 tcatttgaag ttacatgatt atttatatca aaatagtaaa ggtgtcattg ccactgattt
     9781 cgattatgca attccgttgg aaggaaatgc gaagtttctc ggattgattc cgaatccaat
     9841 caactgtgct aaaattgatt ttattcctat ggaaataaaa gataaagttg ttttgttttt
     9901 aggcatcaac aggcacaatt atgttaaaaa aggaattcac ttttttgaag aagcgttgaa
     9961 gattattgat gaaaaatatg ctgctaaagt agaaatcatc atcacggaaa atattcctta
    10021 tgagaaatat attcagttat ataacaaagc tcatattctt ttggaccaag tgtatgctta
    10081 tgatcaagga tacaatgcgt tagaagcaat ggcgaaagga aaagttgttt ttaccggtgc
    10141 tgaacaagcg tttgttgatt attatcagtt aacagatcga gttgccatca atgctttacc
    10201 ggatgtgcat gaattggtaa aagaattatc atttttaatt gaaaacccgc aggaaattat
    10261 caagattggg aaaaatgcac gtgcttttgt tgaaaaggaa catgataatg tgaagattgc
    10321 cgagaagtat
//
LOCUS       DAOTCW010000077        10270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_4278_length_10270_cov_50.409006, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000077 DAOTCW010000000
VERSION     DAOTCW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10270
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4278_length_10270_cov_50.409006"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2264)
                     /locus_tag="P5335_07455"
     CDS             complement(<1..2264)
                     /locus_tag="P5335_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRZ74749.1"
                     /translation="MKKFFTIIGLLFLPTFGFSQNQLWKGYFSYNEIIDLSESSTRLF
                     AACETAIFSKDRINDELKTLNTVDGLSGQTISTIYHSSQYNRTFIGHENGLLIMINEA
                     TGAIQLLVGIRDQTGGIPANKKKINHIYEHAEKLYISCDFGLVEFKLSNMTFGDTFYI
                     SPTGGQTEILETTVLDNQIYAVTLYHGLKKADLSNPNLIDFNAWEVFNPDYWKKIVTF
                     NNQLVAINNNNRVYRFNGNIPSEVLNLGQVGLDIRNREANLIITTASKVFVLNEAFAQ
                     TLLIQNNSIPDLNSNFSCATIIGSTIYIGTLNNGVVETSLDSPSMFDFLLPQGPSKNN
                     IFSITVSPAETLWATYGDYSQFNNPYPLDSYGISKYQENVWLNIPFEELLNAKSIVRI
                     TVNPTNENEIYASSYFSGLLKLENDVATTLFNTTNSGLESLVDPGNPTYGPDIRVEQT
                     AFDKTGNLWVSNGLIANMFKVLKTNGQWQSFSVSNVISGFTSNNRMVVDKNNTKWLAT
                     LDEGLLAFNENYNNKLLKITEGQNEGNLPSSAVQALAVDNRNQLWIGTRKGLRILSSV
                     DRFINQEELTTNSIIINDEGLAQELLFGQFITDIAVDGANNKWVGTADSGVFLFSSNG
                     QETLYHFTTTNSPIPSNVINDVEINGKTGEVFIATAKGMVSFKGTSTKPSDSLEDVYV
                     YPNPVRPGFEGTVKISGLTSKANIKITDIEGNLVHEEISEGGTIEWDTRAFGKYKVAS
                     GVYMIFISSDDALDS"
     gene            complement(2386..3729)
                     /gene="gdhA"
                     /locus_tag="P5335_07460"
     CDS             complement(2386..3729)
                     /gene="gdhA"
                     /locus_tag="P5335_07460"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRZ74750.1"
                     /translation="MTESIQTFVEAVAKKNPNEPEFLQAVKEVAETVIPFIEKNKKYQ
                     NQMLLERMVESERIIMFRVTWIDDAGKTQVNRGYRIQMNSAIGPYKGGIRFHPSVNLS
                     ILKFLAFEQTFKNSLTTLPMGGGKGGSDFDPKGKSDIEIMRFCQSFMTELSKHIGADT
                     DVPAGDIGVGGREVGYMFGQYKRLRNEFTGVLTGKGISFGGSLIRPEATGYGNVYFAQ
                     SMLNTRGESFKGKTVTVSGSGNVAQYAAEKATELGGKVVTLSDSSGYIYDADGIDAEK
                     LAHVMDIKNVQRGRISEYLKKYPNAKFVEGKRPWEVKCDIALPCATQNELNQIEAKAL
                     ISNGCICVSEGANMPSTPEAIEEFTKAKILFAPGKASNAGGVATSGLEMSQNSLRLSW
                     TSEEVDQRLKAIMANIHESCVKYGSDGQGYVDYVKGANIAGFVKVADAMLAQGVV"
     gene            3994..4782
                     /locus_tag="P5335_07465"
     CDS             3994..4782
                     /locus_tag="P5335_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HRZ74751.1"
                     /translation="MPKYLIFTLLLFFGVKNTVMAQFGFSHEVGVIVGPVAFQSDYGV
                     RGDGETNAGNTGFGIGLIHYMNFSYRADCNCYATPTYFNDHFKLRSELSYNKTNLEHF
                     GKWVAPGKTSFAADQLRAMKGSTAVTNIGMQLEYFPFSIREFSATPGKLGPFVSLGGQ
                     FSYYNPEAYSTMGPGLGELLEVTPTKYIDGVTNDGGTVWSVVGSVGTRYKLTILSDLM
                     IDLRWQYYFSNWVDGLNPNPDVYKENKANDWLVWLNFGYIYYLD"
     gene            complement(4842..5987)
                     /locus_tag="P5335_07470"
     CDS             complement(4842..5987)
                     /locus_tag="P5335_07470"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HRZ74752.1"
                     /translation="MKFNTKVIHGGQHHDPSTGAVMPPVYNTSTFAQKSPGVPTNHYE
                     YSRAANPTRQALEDALASIENGVRGLAFSSGLAATDSVLKLLKPGDEVIAMDDLYGGT
                     YRMFTRIYVDFGIKFHFVDMNDLEKFQSLINENTKLVWVETPTNPLMKLADIAAIAKI
                     TNDKKILFAVDNTFATPYLQKPLDLGADIVMHSATKYLGGHSDVIAGALIVRNVELGE
                     KLHFAQFATGGTLGPQDSYMVLRGIKTLHLRVQRHCENGMKVAEFLSNHPEVEKVFYP
                     GLPDHPFHEIAKKQMIGGFGGMVSFTFKSAKKSDSIDFLEKLKVFTLAESLGGVESLA
                     NHPALMTHASIPAEKRLEVGITDDLVRLSVGVEDIDDLLADIEQAFR"
     gene            6122..6514
                     /gene="rbfA"
                     /locus_tag="P5335_07475"
     CDS             6122..6514
                     /gene="rbfA"
                     /locus_tag="P5335_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRZ74753.1"
                     /translation="METNRQKKIGSVLQNDLVDILQGEVRKNGISNLIISVSKVSVTT
                     DLSVAKVYLSVFPTEKGAEILAAIKSNTPLIKHDLSQRVKLQLRKVPNLTFFIDDSLD
                     YIEKIDKALIGDENPIENPELLDKRKKS"
     gene            6516..7715
                     /locus_tag="P5335_07480"
     CDS             6516..7715
                     /locus_tag="P5335_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRZ74754.1"
                     /translation="MNFPLYIAKRYLFSGSKNTAINIITFIASAGIIVGTMALFVVLS
                     VFSGLRDFSLSFANDFDPDLKAVAKIGKTIFITPEQENEISQLEGVANFSKIIEERAL
                     FTFNGKDQLANIKGVDQNFPLVNQVEKTIFQGQWIEENTEQVVVGYGISQKLSMGLFD
                     LVNVFETFVPKPGKGTIDSPEGAFNRSVLVPVGFYFINEELNQKYVFANLNIVQELLD
                     YKSNQISGFELKLKPKADESAIVESLEKIFQNNIIIKNRAQLNESLYKMLNTENLVLY
                     LIFSLVIAVTLFTLIGALIMIIIEKKSNLKTMYSLGSDLSDLRKIFLYQGFLICLIGG
                     IIGLFLGIVLTLLQQKFGFIMINASMAYPVRFSILNIMIVISTIAILGFLASWIASSR
                     VNKALLE"
     gene            complement(7707..8708)
                     /gene="dusB"
                     /locus_tag="P5335_07485"
     CDS             complement(7707..8708)
                     /gene="dusB"
                     /locus_tag="P5335_07485"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRZ74755.1"
                     /translation="MPKIGNIILPDFPLLLAPMEDVSDPPFRRLCKLHGADLMYSEFI
                     SSEGLIRDAMKSRMKLDIFDYERPVGIQIFGGDEEAMAMSAKIVETVQPDLVDINFGC
                     PVKKVVCKGAGAGVLKDVDLMVRLTKAVIRSTNLPVTVKTRLGWDENSINIDEVAERL
                     QDIGVQALTVHARTRAQMYKGHSDWSHIARVKNNPRITMPIFGNGDIDSPEKALEYKN
                     KYGLDGMMIGRAAIGYPWIFNEIKHYFKTGEHLPEPTMKDRIEAARNHLIWSMEWKGE
                     RVGIFEMRRHYTNYFKGIHGFKEYKQRLVTTDGAMALMDVFEEIAAVYENYQTTNLF"
     gene            8856..>10270
                     /gene="lepA"
                     /locus_tag="P5335_07490"
     CDS             8856..>10270
                     /gene="lepA"
                     /locus_tag="P5335_07490"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRZ74756.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLGATQTVTAREEKAQLLDNM
                     DLERERGITIKSHAIQMEYVYKGEKYILNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     AAQSIQAQTISNLYLALENDLEIIPVLNKVDLPSANPEEVSDDIIDLLGCKLEDIIHA
                     SGKTGFGVENILAAIIEKIPAPKGIKDEPLQALIFDSHYNPFRGIEVIFRVKNGEIRK
                     GQKIKFMATGNEYFADEVGTLKLNQVPKDVISTGDVGYLISGIKEAKEVKVGDTLTDA
                     KNPTTNMIQGFEDVKPMVFAGIYPVDTEDYEDLRASMEKLQLNDASLVFVPESSAALG
                     FGFRCGFLGMLHLEIIQERLEREYDMTVITTVPNVSYHAFTKKEPDTILIVNNPSDLP
                     EPSKLDRVEEPYIKATIITKSEFVGNVMSLCIEKRGVITNQTYLTTERVELNFDMPLA
                     EIVFDFYDRLKTVSKGYASFD"
BASE COUNT         3304 a         1833 c         1702 g         3431 t
ORIGIN      
        1 gagtctaaag catcatccga agaaataaaa atcatgtaga ctcccgaagc tactttatat
       61 tttccaaaag ctcgagtatc ccattcaatt gttccacctt cggaaatttc ttcatgcact
      121 agatttcctt caatgtcggt aattttaata ttggctttac tggttaagcc ggaaattttg
      181 acagttcctt caaacccggg acgaaccgga tttggataaa cataaacatc ttccaaggaa
      241 tcgctcggct tggttgaagt tcctttaaaa gaaaccattc cttttgcggt agcgatgaaa
      301 acttctcctg tttttccatt aatttcaaca tcattaatca cattactcgg aattggagaa
      361 ttggtcgtcg taaaatgata aagcgtttcc tgcccatttg aggaaaacag aaaaactcct
      421 gaatcggctg taccaaccca tttattgttt gcaccgtcta cagcaatatc cgtaataaac
      481 tgtccaaata aaagctcttg tgctaaacct tcatcattta taataatgga attcgtagtt
      541 aattcctctt gatttataaa acgatccaca gaagataaaa tacgaagtcc ttttcgggta
      601 ccaatccaga gttgattacg attatctact gccaacgctt gaacagcaga tgacggcaag
      661 tttccttcat tttgtccctc tgtaattttt aataatttat tattgtaatt ctcattgaaa
      721 gctaataatc cttcgtctaa ggtggccagc cattttgtgt tatttttatc aacaaccatt
      781 ctgttattgc ttgtaaatcc gctaattacg ttactaactg aaaaagattg ccattgaccg
      841 tttgttttta aaaccttaaa catattggct attaatccgt ttgaaaccca caaatttccg
      901 gttttatcaa atgcggtttg ttcgacacga atatccgggc cataagttgg atttcccgga
      961 tcaaccaatg attctaatcc actatttgta gtattaaata aggtagtagc tacatcattt
     1021 tccaatttta ataaacctga aaaataagaa ctggcataaa tttcattttc atttgtagga
     1081 ttaacggtta ttctaacaat tgatttagca tttaataatt cctcgaaagg aatatttaac
     1141 catacatttt cttgatattt gctgattcca taagaatcta acggataagg attgttaaat
     1201 tgagaataat ctccataggt cgcccaaagt gtttctgcag gagaaacagt tattgaaaaa
     1261 atattgtttt tagaaggtcc ttgcggtaac aaaaaatcaa acatacttgg gctatccaaa
     1321 gaagtttcta ccactccatt gtttaaagta ccgatataaa tagttgaccc aataatggtg
     1381 gcacaactga aattagaatt aagatcagga atagaattat tttgtatcaa aagagtttga
     1441 gcaaatgctt cattaagcac aaagaccttg gaagcggttg ttatgatcag attagcctct
     1501 ctattcctaa tatctaaacc aacttgtcct aaatttaaaa cttcagaagg tatattaccg
     1561 ttaaatcgat aaactctatt gttattattt atagcaacca gttggttatt aaaagtgaca
     1621 atttttttcc aataatcagg attaaaaact tcccatgcgt tgaaatcaat taaattagga
     1681 ttagataaat ccgctttttt caaaccatga tacaatgtga ccgcataaat ctgattatct
     1741 agcacagttg tttctaaaat ttctgtttga cctcctgtcg gactaatata gaaagtatcc
     1801 ccaaaagtca tattagaaag tttaaattct accaatccaa aatcacaaga aatataaagt
     1861 ttttcagcat gctcatatat atggttgatt tttttcttgt tagccggaat tcctcctgtt
     1921 tgatcacgaa taccaactaa aagttgaatt gcccctgtcg cttcattaat catgattaat
     1981 aaaccatttt catgaccaat aaatgttcta ttatattgac tactatgata aatagttgaa
     2041 atagtttgac cggaaagtcc gtctactgta tttagtgttt ttagttcatc gtttattctg
     2101 tctttcgaaa aaattgccgt ctcacaagca gcaaacaatc gtgtagaaga ttcggaaaga
     2161 tctataattt cattatatga aaaataacct ttccataatt gattttgaga aaatccgaaa
     2221 gttggtaaaa acagtaatcc tatgatagta aaaaatttct tcatccgcat ttataaattg
     2281 acacacaaat atagctaaaa cgaaattata cttcttttaa tatatataaa ggaataaaaa
     2341 aagctgcctt tttagacagc ttttataaaa tattaaagag aaatattaaa caactccttg
     2401 tgcaagcatt gcatctgcaa cttttacaaa tccggcaata tttgctcctt ttacgtaatc
     2461 aacataacct tgaccatctg aaccatattt aacgcaagat tcgtgaatat tagccataat
     2521 tgcttttaat cgttgatcta cttcttctga agtccaactt aaacgtaatg aattttgaga
     2581 catttctaat cctgaagttg ccactccacc cgcatttgat gccttaccgg gagcaaataa
     2641 gatttttgct tttgtaaatt cttcaatagc ctctggtgta gaaggcatgt ttgctccttc
     2701 agaaacacaa atacaaccat tagaaattaa tgctttcgct tctatttgat tcaattcgtt
     2761 ttgtgtagca catggaagtg caatatcaca tttaacttcc caaggacgtt ttccttctac
     2821 aaattttgca ttaggatatt ttttcaaata ttcactgatt ctacctcttt gaacattctt
     2881 aatatccatt acatgtgcca atttttctgc atcaatacca tctgcatcat agatatatcc
     2941 tgaagaatct gaaagtgtaa ctacttttcc tcctaattct gttgcttttt cagcagcata
     3001 ttgagcaaca tttcctgaac cggaaacagt tactgttttt cctttgaaac tttcgcctct
     3061 ggtatttagc atactttggg caaaatatac atttccgtaa cccgttgctt ccggacgaat
     3121 taatgaacct ccgaaagaaa taccttttcc ggttaaaacc ccggtaaact cattacgtaa
     3181 tcttttgtat tgtccaaaca tatatcctac ttctcttcca ccaacaccaa tatcaccggc
     3241 aggaacgtct gtatctgcac caatatgttt tgaaagttcc gtcatgaaac tttgacaaaa
     3301 acgcataatt tctatatcag attttccttt tggatcaaaa tcagaaccac cttttcctcc
     3361 acccattggc aaagtagtca aactgttttt aaacgtttgt tcaaatgcca aaaatttcaa
     3421 aatactcaaa tttacagaag gatgaaaacg aattccacct ttataaggtc caatagctga
     3481 attcatttga attctgtatc ctctattcac ttgagttttt ccggcatcat caatccatgt
     3541 aactcggaac ataatgattc tttccgattc aaccatacgc tctagtagca tttggttttg
     3601 atatttttta tttttttcaa taaaaggaat aacagtttct gcaacttcct ttacagcctg
     3661 taaaaattct ggctcatttg ggtttttttt agctactgct tcaacaaaag tttgaatact
     3721 ttctgtcatg attattagtt tagtaacgtt taagaaaacg ttttcgtttg atgcaacaaa
     3781 tatacatttt attaaaatat tggtgtaatt tattttatat tctcagtaga tttatttttt
     3841 tattaagaat tcatgataaa acatacagtt attgacatga tttataacaa ttattagata
     3901 tttcaatata tatacaagta ttttttcatt ttattttata agtgaattat gtttttatat
     3961 atttgtcgag aattgattat aaactagatg caaatgccca aatatcttat atttacttta
     4021 ctgctatttt ttggagtcaa aaatactgtc atggctcaat ttggtttttc acatgaagtt
     4081 ggagttattg ttgggcctgt tgcttttcaa tcggactatg gggtacgggg tgatggcgaa
     4141 acaaatgcag ggaataccgg atttggtatt ggattaattc actacatgaa cttttcatat
     4201 cgtgcagatt gtaattgtta tgccacacca acttatttta atgaccactt taaattgaga
     4261 agtgaactat cctacaataa aacaaattta gaacactttg gaaaatgggt tgcccctggt
     4321 aaaacttcat ttgctgcaga tcaattaaga gcaatgaaag gaagtactgc agtaaccaat
     4381 attggtatgc agttggaata ttttccattt agtattagag aattttctgc aactccaggt
     4441 aaattaggtc catttgttag tttaggaggg caatttagtt actacaatcc tgaagcttat
     4501 tcaactatgg gtcctggatt aggagaattg cttgaagtga ctccaacgaa atatattgat
     4561 ggtgtaacaa atgatggtgg aacagtatgg tctgttgttg gaagtgttgg aacacgttat
     4621 aaactaacca ttctttcaga tttaatgatt gatttaagat ggcaatatta tttttctaat
     4681 tgggtggatg gtttaaatcc aaatcctgat gtatataaag aaaataaagc caatgattgg
     4741 ttggtttggt taaattttgg atatatttat tatttagatt agtacaaatt aactaaaaaa
     4801 agaaaccccg taaagtgata ttttacgggg tttctttttt attatctaaa ggcttgttca
     4861 atatcagcaa gcaagtcatc aatatcttca acgccaacgc ttaatcgcac taagtcatct
     4921 gttattccaa cttctaacct tttttctgcg ggaatagaag cgtgtgtcat caatgccggg
     4981 tgatttgcta atgattctac tccacccaga gattctgcca aagtaaatac ttttagtttt
     5041 tctaaaaagt caattgaatc tgatttttta gctgatttaa aggtaaatga aaccatccct
     5101 ccaaaaccac ctatcatttg cttttttgca atttcgtgaa aaggatgatc aggtaatcct
     5161 ggataaaaaa ctttttcaac ttctggatga ttacttaaaa attcagctac cttcatgcca
     5221 ttttcacaat gtctttggac tctaagatga agtgttttta ttcctctcaa gaccatataa
     5281 ctatcttgag gacctaaagt tccacccgtt gcaaattgag cgaaatgtaa tttttcgcct
     5341 aattctacat ttcttacaat taaagccccg gcaattacat cagaatgtcc tcctaaatat
     5401 ttagttgcgg aatgcatcac aatatccgcc cctaaatcca atggtttttg aagataaggg
     5461 gttgcaaaag tattgtctac agcgaataag atttttttat cattggtaat tttagctatg
     5521 gctgcaatat ctgctaattt catcaaaggg tttgtaggtg tttcaaccca aaccaatttt
     5581 gtgttttcat ttatcaatga ctgaaatttt tccaaatcat tcatgtctac aaaatgaaat
     5641 ttaatcccaa aatcgacata aatcctagta aacattcgat aagtacctcc atataaatca
     5701 tccattgcta taacttcatc acctggcttc aaaagtttta atacggagtc tgtcgcagct
     5761 agtccggaag aaaaagctaa accacgaact ccgttttcaa tactcgccaa agcatcttct
     5821 aatgcttgcc gcgttggatt agccgctctg ctatattcat aatgattagt tggtacaccc
     5881 ggactttttt gagcaaaagt cgacgtatta taaaccggcg gcataactgc acccgtactt
     5941 ggatcgtggt gttgacctcc atgaattact tttgtattga atttcatatt gttatttatt
     6001 tgtgcaaaga taagaagttg aatttaggca gtaaagtgta agttcataaa gattatataa
     6061 ataatttcat ccgcctttct aagataactg ttttcaaaat taaaatctgt atttttgcat
     6121 tatggaaaca aatagacaaa agaaaatagg cagtgtttta caaaatgatt tagtagacat
     6181 tttgcaaggt gaagttcgta aaaacggaat atccaatttg attatttccg tttcaaaagt
     6241 gtctgtaact accgatttat cagtagctaa agtatatttg agtgtttttc caacagaaaa
     6301 aggagcagaa attttagcgg ctattaaatc aaatacacca ttaattaaac atgatttgtc
     6361 gcaacgcgta aaacttcaat tacgtaaagt acctaattta acgttcttca ttgatgattc
     6421 attggattat attgaaaaaa tagacaaggc attaattggc gatgaaaatc caattgaaaa
     6481 tcctgaactt ttagataaaa gaaaaaaatc gtaaggtgaa ttttccgctt tacatagcca
     6541 aacggtattt atttagcggt agtaaaaata cggcaatcaa catcattact tttattgcct
     6601 ccgccggaat cattgtgggt acgatggcac tctttgtcgt attatccgta tttagcgggc
     6661 ttagagattt tagtctgtcg tttgctaatg attttgaccc ggatttaaaa gcagtcgcta
     6721 aaatcggaaa aactatcttt atcactcctg aacaggaaaa tgaaatttca caactcgaag
     6781 gtgttgcaaa ctttagcaaa attattgaag aaagagccct ctttaccttt aatggaaaag
     6841 atcaattagc caatatcaaa ggggttgatc aaaatttccc tctagttaat caagtagaaa
     6901 aaaccatttt tcaaggacaa tggatagaag aaaacactga gcaagttgtg gttggctacg
     6961 gtattagtca aaaactttcc atgggattat ttgatttggt caatgtcttt gaaacatttg
     7021 ttccaaaacc gggaaaaggt actattgact cacccgaggg agcttttaat cgatccgttt
     7081 tagttcctgt tggattctat tttattaatg aagaacttaa tcaaaaatat gtttttgcca
     7141 atttaaatat tgtgcaggaa ttattggatt ataaatccaa tcaaatttcg ggttttgaat
     7201 tgaaactcaa accaaaagct gatgaatcag ctattgttga atcgttagaa aaaatttttc
     7261 aaaataatat aatcatcaaa aacagagctc aattaaatga gtcgctttac aaaatgctaa
     7321 ataccgaaaa tctggttttg tacctcattt tttctttggt cattgctgtt acacttttca
     7381 ccttaattgg agcattaatc atgataatca ttgaaaagaa atcgaaccta aagactatgt
     7441 atagtttagg gtcagattta agtgatttga gaaaaatttt tctttaccaa ggctttttaa
     7501 tttgtttgat tgggggaata attggtttgt ttttaggaat agtactgaca ctcctccaac
     7561 aaaaatttgg ttttattatg ataaatgcca gcatggctta tcctgtccgc ttttcaatat
     7621 taaatattat gattgtgatt agtacaattg ccatattagg ctttttagct tcttggattg
     7681 ccagcagccg agtaaataaa gcccttttag aataaattgg tggtttgata gttttcataa
     7741 accgcggcaa tttcttcaaa cacatccatt aaagccattg ccccatctgt agtaactaag
     7801 cgttgtttat attctttaaa accatgaatc cctttgaagt aattggtata gtggcgacgc
     7861 atttcaaaaa ttccaacgcg ttctcctttc cattccattg accaaattaa gtgatttcta
     7921 gcggcttcaa tacggtcttt catcgtgggt tctggtaaat gctcgccagt tttaaaataa
     7981 tgtttgattt cgttaaaaat ccatggataa ccaatggcag cacgaccaat catcattccg
     8041 tcaagaccat atttattttt atattccaat gctttttcgg gtgaatcgat atcaccatta
     8101 ccaaaaatgg gcatcgtaat tctcggattg ttttttactc gggcaatatg cgaccaatcg
     8161 gaatgacctt tatacatttg agctctcgtc cgggcatgga ccgtcaaagc ttggacacca
     8221 atatcttgca gtcgttcggc tacttcatca atgttgatgg aattctcatc ccaccccaaa
     8281 cgtgttttta ccgtaacagg aagattggta ctacgaataa ccgctttcgt taaccttacc
     8341 attaaatcta catcttttaa aaccccagct ccggcacctt tacaaaccac ttttttcacg
     8401 ggacaaccaa aattgatatc caccaaatca ggctgcacag tttccacgat tttggcactc
     8461 atagccatgg cttcttcatc acctccaaaa atttgaatac ctacaggacg ttcataatca
     8521 aaaatatcca atttcattct gcttttcatc gcatcacgaa tcaatccttc ggatgatata
     8581 aattctgagt acatcaaatc tgctccgtgt aatttgcaca agcgacgaaa cggcggatca
     8641 ctcacatctt ccattggagc aagaagcagg ggaaagtcgg gcaagatgat gtttccgatt
     8701 tttggcattg gaattaaatt ttgggcaaag atagttaaac ctgacaggtt tacaaaaacc
     8761 ctacttgttt tgcaggtttg aaatccaaaa ctttacttta tatttgcgga ttaaatgcac
     8821 tcagcattcg cattagaaat tgatagatta cgaagatgaa gaacatacga aatttttgca
     8881 ttattgctca cattgaccac ggaaaaagta cgttggctga ccgtctattg ggagcaacac
     8941 aaaccgtaac ggctcgtgag gagaaagcac aattgcttga caacatggat ttagagcgtg
     9001 aacgcggaat taccattaag agtcacgcca tacaaatgga atatgtttat aaaggtgaaa
     9061 aatatattct gaatttgatt gatacacctg gacacgttga tttttcctat gaagtttcgc
     9121 ggtctattgc ggcttgtgaa ggagctttat tgattgttga tgctgctcaa agtattcaag
     9181 ctcaaaccat ttcaaattta tatttagcct tagaaaacga cttggaaata attcctgtgt
     9241 tgaacaaagt ggatttgcca agtgccaatc cggaggaagt gagtgacgat attattgatt
     9301 tattaggttg caaattagaa gatattattc atgcttcggg caaaaccggt tttggggtag
     9361 aaaatatttt ggcagcgatt attgaaaaaa ttcccgctcc aaaaggcatt aaagacgaac
     9421 ccttacaagc attaattttc gactcccatt ataatccgtt tcgaggcatt gaagtaatct
     9481 ttcgtgtaaa aaatggtgaa atccgaaaag gtcagaaaat taaattcatg gccaccggaa
     9541 atgaatattt tgctgatgaa gtgggtacct taaagctgaa tcaagtgcca aaagacgtga
     9601 tttcaaccgg agatgttggc tatttaatat caggaataaa agaagcgaaa gaagtaaaag
     9661 taggtgatac attgaccgat gctaaaaatc ctacgacaaa tatgattcaa ggtttcgagg
     9721 atgtaaaacc aatggttttt gccggaattt atcccgttga caccgaagat tatgaagatt
     9781 tgcgggcttc aatggaaaaa ttgcaactaa acgatgcatc gttggttttt gttcctgaaa
     9841 gttctgctgc tttaggtttt ggtttccgtt gtggattctt aggaatgttg catttagaaa
     9901 tcattcaaga acgcttggag cgtgagtacg acatgaccgt gataacaacg gttcccaacg
     9961 tttcctatca tgcattcact aaaaaagaac ctgacactat tttgattgta aacaatccgt
    10021 ccgatttgcc ggaaccctca aaattagacc gcgttgaaga accgtatatt aaggcaacaa
    10081 ttattaccaa atcagaattt gttggtaatg taatgagttt atgtatagaa aaaaggggcg
    10141 taataactaa tcaaacttat ttaaccaccg aacgtgttga attaaatttt gatatgccgt
    10201 tggccgaaat tgtattcgat ttttatgacc gattgaaaac ggtttccaaa ggatatgctt
    10261 cgtttgacta
//
LOCUS       DAOTCW010000078         9995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_4481_length_9995_cov_38.400704, whole genome shotgun sequence.
ACCESSION   DAOTCW010000078 DAOTCW010000000
VERSION     DAOTCW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4481_length_9995_cov_38.400704"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1315)
                     /locus_tag="P5335_07495"
     CDS             complement(50..1315)
                     /locus_tag="P5335_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ74757.1"
                     /translation="MKKTLLLLVLTIALNAKAQDTDNLFTIVTKYSSEFGGSTRMGNV
                     NPTVGFVNNIGTATSPNLFSVTGGSVNQTTNRFNLVSTNFFMSFDLFDGSIQDNLPIN
                     SFFEGTTLFSNVRYNNSNNTLYGLASTFTENNELIGMYLASLNTETGDLTALSQNSVG
                     NGYQLAGTAVNPLEMVYYYSTGPQFVGLDLYNGTIYSNPTVQYSNPNEFNFTNFAYNC
                     ADDTMYGLVTEDTQVPNPNSPSGVSIYAMRFGKINPATGEVTNISDVPLPTSYYSASA
                     SSTIDPNTNTYYYSDGSNIYGISLLTGLVVSTAPLVFEDGQNINMMINYNNCLGAIAT
                     RQDPALSVPQNTLEDKITVYPNPTSNTLSIKAPFAIDSIEVIDSNGRTVQAPLIEANT
                     VQVQALQSGVYFLKIVSNNRVKNIKFLKI"
     assembly_gap    1443..1542
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1543..2204)
                     /locus_tag="P5335_07500"
     CDS             complement(<1543..2204)
                     /locus_tag="P5335_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRZ74758.1"
                     /translation="MKQITSPQNPFIKSLVQLQEKAKIRKQTGTFLMEGKREIELALK
                     GGYQLETILYLPELVSDKELRAFSGCDCIEISKEVYQKLAYRDTTEGILAVAKTKSHL
                     LTDLHLSDNPLILVAEGIEKPGNLGALLRTADAANSDAVIIANPKSDLYNPNIVRSSV
                     GCLFTNQIATGTTEEVIAFLKAKKVAIYSATLQNANHYHLENFTSSTALVVGTEATGL
                     TEP"
     gene            complement(2242..3576)
                     /locus_tag="P5335_07505"
     CDS             complement(2242..3576)
                     /locus_tag="P5335_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRZ74759.1"
                     /translation="MKRLFRQLYITNFFFYVLAGIIALFAVSFLFPVLYNLAWYFLLL
                     LLTFFLLDCIILFGAKTGMEATRISPEKLSNGDENPIRISIRNYYTFSIFTKIIDEIP
                     FQFQVRNFNINRKVKASSQDDIQYFLRPVERGEYTFGNLNVYVSSPLKIIARRFTFNK
                     DQMVPTYPSYIQLRKYDLMAFSNNLFQYGVKKIRRIGHTMEFEQIKEYVQGDDIRTLN
                     WKATAKKNSLMVNQFQDEKSQSVYMIIDKGRVMKMPFNGLSLLDYAINATLVLSNVIL
                     KKHDKAGMFSFSKKVENRVVAEKRSSQMQLIMECLYNIKTDFFESDFSRLYTDIKKNI
                     NQRSLLLLYTNFETLDGLHRQLPYLKGIAKNHLLVVVFFQNTELNDIIHNKAETVQEV
                     YDKVIAEKFAFEKRLIVNELKKYGIYSVLTQPENLTLDTINKYLEIKARGIL"
     gene            complement(3595..>4569)
                     /locus_tag="P5335_07510"
     CDS             complement(3595..>4569)
                     /locus_tag="P5335_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRZ74760.1"
                     /translation="DNVNFESRIDLKPLQDSIHAIKQELNSVIVGQHKMIDQLLVAIL
                     SNGHVLLEGVPGVAKTITAKLLAKTLSIGFSRIQFTPDLMPSDILGTSVFDLKKSEFE
                     FKKGPIFSNLILIDEINRAPAKTQAALFEVMEERQITLDGTTYQMTAPFLVIATQNPI
                     EQEGTYRLPEAQLDRFLFKINIDYPKLDEEITILQKEHALLSNNKLDHIKTHLTAADI
                     QHYQELVKQVLVEPNLLEYIAKLVVNTRENGFLYLGASPRASIAILNAAKGYAALRGR
                     DFVTPEDIKEAAIPVLQHRVIVTPEREMEGISSKEIIQQIIESIEIPR"
     assembly_gap    4570..4669
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4670..5868)
                     /locus_tag="P5335_07515"
     CDS             complement(<4670..5868)
                     /locus_tag="P5335_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HRZ74761.1"
                     /translation="MNKTLKIYIGILVALMVLIVFVDANRPKPINWTPSFDVNSKIPF
                     GLYVFNEEKDTYFKGDTIQTIQSTPYEFLDAFYDYDTLVDTYTTNGTFLAIDSDYTID
                     ETSTEELLLFVSHGNDAFLSMNTFSTTLEDSLGFSYSNLYFQEQLEVSLANPTLSKKS
                     FEMKMGVNGYHFTSFDTLKTTVLGYQKIDKKSQVNFIKVPYGKGNFYLHLQPFAFTNY
                     YLLKNKNAEYAEKVLSYIPKGNLFWYPNQQYKSGISSSPLRFILSQEGLRWAWYLFLI
                     GMIIFMIFNAKRKQRIIPIIKPLTNTTVDFTKTIGNLYFQEGNHDTIMEKKIIYFLEK
                     IRQDYLLDTQNLDDDFIKKLHQKSGKEMALIEKVVTLIKRQRKTFKSTEAELIELNAV
                     IEKVLET"
     gene            complement(5869..6627)
                     /locus_tag="P5335_07520"
     CDS             complement(5869..6627)
                     /locus_tag="P5335_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HRZ74762.1"
                     /translation="MNKLLYIAFLLTWMTASSQDSLVVDTTTVAKIIFTEKDIQIDSL
                     SMEARSFETTIKEKYKSDEFVYEQKVSKENWWDRFKEWLSDFFRRLFNFSSDDVSMTF
                     VEVLIKVLAVLLIVFVIYLIVKAIMNGEGQWIFGKSSDKKVIHHEDVEKNIHLIDFEK
                     LIKDTLKSGEKRLSIRYYYLWLLKKMAEKNIIEWDVEKTNTDYLYEIKEEAFKTKFAY
                     VSYLYNYSWYGEFELDEETFLKAKAAFEKTIQSI"
     gene            complement(6620..7561)
                     /locus_tag="P5335_07525"
     CDS             complement(6620..7561)
                     /locus_tag="P5335_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74763.1"
                     /translation="MLHLFKKRTFSEYIGDTFAFFKENGKHFFKHYFIINGGLLLLLL
                     VLSYFIFKVYFEVLFSSIGTNAPNFLEDYFNNNIALIIGVFLFFLIVILFVSLVNYAF
                     PIIYLQLYEKHKGCNFETLDLIKAMKAKIGKIILFFLALVGLSLTAGLLLMAIVFALF
                     FVIIGIPLAIIGIPALMAWVQLSFYEYLNEDMGILEAYGRGFDKLKQQFWPIVGSTLI
                     MYMIIQTIATVFIAIPYIIGVIDMVTNIENQQTDADAFSFFSIMMVIVMCISILVSYI
                     LNNIMIINGGIIYYSLRDQNENITTLSDIDQIGTPSE"
     gene            complement(7565..8542)
                     /locus_tag="P5335_07530"
     CDS             complement(7565..8542)
                     /locus_tag="P5335_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HRZ74764.1"
                     /translation="MREVAFIKQNKEKWLEFEQAIFGKAKKNPDEMASLYIHLVNDLS
                     YAQTYYPKSKTVVYLNYLASQIYQKIYKTKRTEQNRIIYFFSEEVPLIVYHYRRYLVY
                     AFVLFFLCTGIGVLSARYDLDFVRLILGDDYVNMTLENIKEGNPVAVYKSGSNWGGFI
                     AITMNNLYVGAQCFIWGIFLGIGTFYIMLQNCIMLGSFQYFFYQEGVFWESVQGIWIH
                     GSMEIFAIVIETAAGFILGASILFPKTYSRMNSFKIGFKDSLKIFLSTIPFTIAAGFL
                     EGFVTRYALEMPKWLNVVIILGTLSLISFYFLLFPFIVHRKKNLNIPTI"
     gene            8563..9306
                     /locus_tag="P5335_07535"
     CDS             8563..9306
                     /locus_tag="P5335_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRZ74765.1"
                     /translation="MSELSITTTQNVNINFTAATVADRMLAYVVDLLIKGAYVVVVWG
                     IFFYALGINQGMTGLDDWSYYGLVVLFYSPVIFYSLTFESLLEGQTIGKRLIKIKVVK
                     IDGYQASFGDYLMRWLFRIIDVNLSSGVVGLIAMITSSKTQRLGDMTAGTAVISLKNN
                     ININHTILEEIDQAYVPTYPLVIKLSDNDARIIKETFTVAHIKRDFATLIKLRTKIEE
                     VTGIKNISGNDTDFIRTVLKDYNYYTQNM"
     gene            9348..9950
                     /locus_tag="P5335_07540"
     CDS             9348..9950
                     /locus_tag="P5335_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRZ74766.1"
                     /translation="MFHLLDIIGTIAFAISGVLTAFNKKLDPFGVYIIAFVTAVGGGT
                     LRDVLIGRTPVVWMQDITYVYMITIGFFIALVFRKKLDKLRTSLFLFDTIGLGVFTLI
                     GLERGIEAGLHPIICIALGTMTACFGGVIRDILCNEIPIIFRREIYATICIIGGLLFF
                     ALKHFNLNNDVLYLVTSIVIITIRLMAVKFKWYLPTMGRV"
BASE COUNT         3050 a         1809 c         1829 g         3107 t
ORIGIN      
        1 tcaattttag caatttttcc cactttattt taaaatgaga tttgcccctt tatatcttca
       61 aaaactttat gtttttaaca cgattatttg aaacaatctt taagaaataa acgccacttt
      121 gtaaggcttg cacttgcact gtatttgctt caatcagtgg agcttgtacc gttctaccgt
      181 tactgtctat cacttcaatc gaatcaatgg caaaaggagc tttgatagaa agggtgtttg
      241 aagttggatt agggtaaaca gtgattttat cttccaatgt attttgagga acggaaagag
      301 caggatcttg tcttgtggca atagcaccca ggcaattatt gtagttaatc atcatgttga
      361 tgttttgacc gtcttcaaat acgaggggag cagtgcttac gacaagtccg gttagtaatg
      421 aaattccgta gatgttgctt ccatcgctat agtagtacgt attggtatta ggatcgatgg
      481 tagagcttgc acttgctgaa taataggagg tgggtagtgg aacatcggaa atgttggtta
      541 cttcgcctgt tgcaggatta atttttccga aacgcatggc gtaaatgctc acaccggaag
      601 gggaattagg gttgggcact tgcgtatctt ccgttactaa tccatacatg gtatcatccg
      661 cacaattata ggcaaaattg gtaaaattaa attcatttgg attgctgtat tgcacggtag
      721 ggttgctgta aattgttccg ttgtataaat caagtccaac aaattgagga ccggtggagt
      781 aataataaac catctctaac ggattgacag ccgttccggc taattgatat ccatttccga
      841 cagaattttg agaaagggca gttaaatcac ctgtttctgt gtttaaacta gccaaataca
      901 tgccgattaa ttcgttattc tcggtgaaag tagaagctaa accatagagt gtattgttcg
      961 aattattata tcgaacgttt gaaaataagg ttgtgccttc aaagaaacta ttaataggca
     1021 aattgtcttg aatggaaccg tcgaagaggt cgaaggacat gaaaaaattg gtggagacca
     1081 aattaaaccg attggttgtt tgattgacag aaccaccggt gacactaaaa agatttgggc
     1141 tggtcgctgt gcctatatta tttacaaatc caaccgttgg gtttacatta cccattcgag
     1201 tgcttcctcc aaattcagaa gagtacttcg ttactatcgt aaataaatta tcagtatcct
     1261 gagccttggc attcaatgct attgtaagta caagaaggag taaagttttt ttcatagcgt
     1321 gagtttttag cttctttagt tgagcctatt gttttgatag acaactgata agaatgtata
     1381 aatttatgat ttaattttat gttaattata aattgacacg catttaacta ttgttgaacc
     1441 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggctcggt taatccggtt
     1561 gcttctgtgc caacgaccaa ggccgtggaa gaagtgaaat tttctaaatg atagtgatta
     1621 gcgttttgca aagtggcgga atagatggct actttttttg cttttaaaaa agcaattact
     1681 tcctctgtag tgcctgttgc aatttggttg gtaaacaaac agccaacact cgaacgcacg
     1741 atgtttggat tgtataaatc actttttgga ttagcaatga ttacggcatc actatttgcg
     1801 gcatcagcgg tgcggagtaa agctcctaaa ttacccggtt tttcaattcc ttctgccact
     1861 aaaatcaagg gattatcaga aagatgcaaa tcagttaaaa gatgcgattt cgtttttgct
     1921 acggctaaaa tgccttccgt tgtatcgcga taggctaact tttgataaac ttcttttgag
     1981 atttcgatac aatcgcaacc ggaaaaagca cgtagttctt tatcagaaac taattcgggt
     2041 aaatatagaa tggtttccaa ttgataaccg ccttttaacg ccaattcgat ttcgcgtttg
     2101 ccttccatca aaaatgttcc ggtttgctta cggattttag ctttctcctg caattgtaca
     2161 agagatttga taaaagggtt ttgaggggaa gtgatttgtt tcaaggtgct gaggttctga
     2221 gtggcaaagt tactttgttt tttacaaaat tcctcgagct ttgatttcta aatacttatt
     2281 gatggtatct aacgtcaaat tttcgggttg ggttaaaaca gaataaatgc cgtatttttt
     2341 caattcgtta acgattaaac gtttttcaaa ggcaaacttt tcagcaatta ctttgtcata
     2401 cacttcctga acggtttcgg ctttgttgtg aattatgtcg ttgagttctg tgttttgaaa
     2461 aaagaccacc accaacaaat ggtttttagc aatgcctttt aaatagggca attggcgatg
     2521 caatccgtcc aaggtttcaa agttggtata taacaaaagt agactccgtt ggttaatgtt
     2581 ctttttgatg tcggtgtata atcggctaaa atcactttcg aaaaaatcag ttttgatgtt
     2641 atacaaacac tccatgatta attgcatttg tgaggagcgt ttttcagcca cgacacgatt
     2701 ttcaaccttt ttagaaaaag aaaacattcc cgctttatcg tgttttttca aaattacgtt
     2761 cgataaaact aacgttgcgt tgatggcata atccaataaa ctcaatccgt tgaaaggcat
     2821 tttcatcaca cggcctttgt caataatcat ataaaccgat tgtgattttt catcttgaaa
     2881 ttggttcacc atcaagctgt ttttcttggc ggttgctttc caattcaggg ttcggatgtc
     2941 atcgccttga acgtattctt tgatttgttc aaattccatg gtatgcccga ttcgacgtat
     3001 tttctttaca ccatattgaa ataaattatt cgaaaaagcc atcaaatcat atttccgcaa
     3061 ttggatatac gacgggtagg tgggcaccat ttgatccttg ttgaatgtaa aacgacgagc
     3121 gattattttt aacggagaag aaacataaac attaaggttc ccgaacgtgt attcccctcg
     3181 ttccaccgga cgtaggaaat actgaatgtc atcttgtgaa gacgctttca cttttcggtt
     3241 gatattaaaa ttccgtacct gaaattgaaa aggtatttcg tcaatgattt tagtgaatat
     3301 ggaaaacgta taataattcc gaatagaaat ccgaatgggg ttttcatctc cattagacaa
     3361 tttttccgga ctgatgcggg tggcttccat tccggttttg gctccaaata aaatgataca
     3421 atctaacaaa aagaaggtca gtaaaagcaa taaaaaatac cacgctaaat tgtataacac
     3481 cggaaacaaa aaactcacag caaagagggc tatgatgcct gccagcacat agaaaaagaa
     3541 attagtgata tacaattgcc tgaataatcg tttcaaaata ggaaatttta taagttatct
     3601 cggtatctca atcgactcaa tgatttgttg aatgatttct ttgctgctga tgccttccat
     3661 ttcgcgttcc ggggtaacaa tgacccgatg ttgcaaaaca ggaatagcgg cttctttgat
     3721 gtcttccgga gtaacaaaat cgcgtccgcg aagggcagca taccctttag cagcatttag
     3781 aatagcgatg gaggctctcg gggaagcacc taaataaagg aacccatttt cacgcgtgtt
     3841 gaccactagt ttagcgatgt attctagtag gtttggttca accagaacct gcttcaccag
     3901 ctcttgataa tgttgaatat cggcagcggt aaggtgtgtt ttaatatggt ctaatttatt
     3961 attgctcaac aacgcatgtt ctttttgaag gatggtgatt tcttcgtcta atttgggata
     4021 atcaatgttg attttaaaca gaaatcggtc caactgagct tcaggtaaac gatacgttcc
     4081 ttcctgttca attgggtttt gggtggcaat cactaaaaac ggagcggtca tttgataggt
     4141 tgttccgtct aacgtaattt gacgttcttc cattacttca aaaagagcgg cttgtgtttt
     4201 ggcaggagcc ctattgattt cgtcaatcaa aatcaggttg gaaaagatgg gccctttttt
     4261 aaattcaaac tccgatttct ttaagtcgaa aacagaagtt cctaaaatat cagatggcat
     4321 taaatccggc gtaaattgta tgcggctaaa accgatgctt aatgttttgg ccagcaattt
     4381 tgctgtaatc gttttggcta ccccgggaac accttccagc aacacatgac cattggataa
     4441 aattgccaca agcagttggt ctatcatttt gtgttggcct acaatcacgg aattaagttc
     4501 ctgcttgatg gcatgaatac tatcttgaag cggtttcaaa tcaattcggg attcaaagtt
     4561 tacgttgtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ttgtttctaa
     4681 aactttctct atgacggcat tcaattctat taattcggct tcggtgcttt taaatgtttt
     4741 tcgttgccgt ttaatcagtg tcactacttt ttcaatcaaa gccatttctt ttcctgattt
     4801 ttgatgtagc tttttaataa aatcatcatc caggttttgt gtatccagta aataatcttg
     4861 cctgattttt tctaaaaagt agataatctt cttttccatg atggtgtcat gatttccttc
     4921 ctgaaaatac agatttccaa tggttttagt aaaatcaacg gttgtgtttg tcaagggttt
     4981 aattattgga atgatacgtt gttttcgttt agcattgaaa atcataaaga taatcattcc
     5041 gattaaaaat aaataccatg cccatcgcaa cccctcctga cttaaaataa accgcaacgg
     5101 agagctggaa atacctgatt tatactgctg attcggatac caaaatagat ttcccttcgg
     5161 aatataggac aatacttttt cagcatattc tgcattttta tttttcaata aataatagtt
     5221 ggtaaacgca aagggctgca aatgcaagta gaaattccct tttccgtaag gcacttttat
     5281 aaaattaact tgtgattttt tatcaatttt ctgataacct aaaacagttg tttttaacgt
     5341 gtcaaatgag gtaaaatgat atccgtttac acccattttc atttcaaatg actttttgct
     5401 aagcgttgga ttagccaggc tcacttcaag ttgctcttga aaatacaggt tggaataact
     5461 aaagcccaac gaatcctcta atgttgttga aaaagtgttc atgctcaaaa aagcatcgtt
     5521 tccgtggctc acaaacagca ataactcttc agtagaagtt tcgtcaatag tatagtcgga
     5581 atcaatcgct aaaaaagttc cattggtggt ataagtatcc actaacgtat cataatcata
     5641 aaacgcatct aaaaactcat aaggcgttga ttgaatggtt tgaatagtat cgcctttaaa
     5701 ataagtgtct ttttcttcat taaaaacata taatccaaac ggaatttttg aattcacatc
     5761 aaagcttgga gtccaattaa tcggtttcgg tcgattagca tccacaaaaa cgatgagcac
     5821 catgagtgcc actaaaatac caatgtatat tttcagtgtt ttattcatct aaatggattg
     5881 tattgttttt tcaaaagcgg ctttagcttt caaaaaggtt tcttcatcca gctcaaattc
     5941 accataccaa ctataattgt atagataaga aacataggca aacttagttt tgaaagcctc
     6001 ctcttttatt tcatataaat aatcggtatt tgttttttca acatcccatt caatgatgtt
     6061 tttttcggcc atctttttca atagccaaag gtaataatag cgaatgctca atcgcttttc
     6121 gcctgatttt aaggtatctt taattagctt ttcaaaatca attaaatgaa tgtttttttc
     6181 aacatcttca tggtgaatta cttttttatc agatgatttt ccaaaaatcc actgtccttc
     6241 accattcata atggctttaa cgatgaggta aatcacaaaa acaatgagta agacggccaa
     6301 caccttaatc aaaacttcca caaacgtcat cgaaacgtca tcactgctga aattaaacaa
     6361 acgacggaaa aagtcggaaa gccattcttt aaatcggtcc caccagtttt ctttgctcac
     6421 tttttgttca taaacaaatt catcgctttt atatttttct ttaatggtgg tttcgaaact
     6481 tctggcttcc atcgaaaggg aatctatttg aatatccttt tcagtgaaaa taattttcgc
     6541 aaccgttgtg gtatcaacca caagactgtc ttgcgatgaa gccgtcatcc aagttaataa
     6601 aaaagctatg taaagaagtt tattcacttg gggtaccaat ttggtctatg tcgcttaacg
     6661 tcgttatgtt ttcattttga tcccgtaagc tgtaataaat aattccgcca ttaataatca
     6721 taatgttgtt tagaatatag ctcaccaaaa tactgatgca catcacaata accatcatga
     6781 tggaaaagaa agaaaaagca tcggcatcgg tttgttggtt ttcaatgttg gtcaccatgt
     6841 caatcacgcc aataatgtag ggaatagcta taaaaacagt cgctatcgtt tgaatgatca
     6901 tatacataat tagggttgaa ccaacgatgg gccaaaactg ttgtttcagt ttgtcaaacc
     6961 ctcttccata agcttctaaa atgcccatgt cttcgttgag gtattcataa aaactgagtt
     7021 gaacccaagc cataagagcc ggaattccaa taatggcaag cggaattccg atgataacaa
     7081 aaaacaaagc aaaaacaatc gccatcaaaa gtagtccggc ggttaaactc aatcctacca
     7141 aggctaagaa aaacagaatg atcttgccga ttttagcttt cattgctttt ataaggtcaa
     7201 gcgtttcaaa attacagcct ttgtgttttt catacaattg caaatagata atcggaaaag
     7261 cgtaattgac gagcgaaaca aaaagaatga cgattaaaaa aaatagaaac acgccgataa
     7321 tcaacgcaat attgttgttg aaataatctt ctaaaaagtt gggagcattc gtaccgatgg
     7381 aggaaaaaag aacttcaaaa taaactttaa aaataaaata ggaaagcacc aataaaagca
     7441 acaataatcc accgttgatg atgaaatagt gtttaaaaaa atgttttccg ttttctttaa
     7501 aaaaagcaaa cgtatcgccg atatattcgc taaaagttct tttcttgaat aggtgtagca
     7561 tagtttaaat tgttggaatg ttgaggttct tttttcgatg cacaataaat ggaaacaata
     7621 aaaaataaaa ggaaataaga cttaacgttc ccagaataat cacaacattc agccacttgg
     7681 gcatttcaag tgcataacgg gttacaaaac cttctaaaaa gccggcggca atcgtaaaag
     7741 gaatcgtgct caaaaatatt tttaaactgt ctttaaatcc aatcttaaaa gaattcatcc
     7801 gcgagtaagt tttaggaaat aggatagaag ccccaagaat aaatccggca gcggtttcaa
     7861 tcacaatggc aaaaatctcc atcgaaccgt gaatccaaat tccctgaacg ctttcccaaa
     7921 aaacaccttc ctgataaaag aaatattgaa aagaacccag catgatgcag ttttgaagca
     7981 tgatgtaaaa cgttccgatg cctaaaaaaa tcccccaaat aaaacactga gcacccacat
     8041 aaaggttgtt catcgtgatg gcaataaatc ctccccagtt gctgccgctt ttataaaccg
     8101 ccaccgggtt accttctttg atgttttcca gcgtcatgtt cacataatca tcccctaaaa
     8161 tcaaacgaac aaagtctaaa tcatagcggg cagacaaaac cccaatgccg gtgcagagaa
     8221 aaaataaaac aaaagcatac actaaatacc gccgataatg atagacaatc aacggaactt
     8281 cctctgaaaa gaaatagatt attctgtttt gttcggttcg tttggtctta taaatcttct
     8341 gataaatttg agaagcaagg taatttaagt acacaaccgt cttactttta gggtaatacg
     8401 tttgggcata cgataaatcg ttgactaaat ggatatataa actagccatt tcatcaggat
     8461 tttttttagc tttaccaaaa atagcttgtt caaattcaag ccatttttct ttattttgtt
     8521 ttatgaaggc gacttctctc atggattgct aaaatataaa atatgtcaga attatcgatt
     8581 acaacaacac aaaatgtgaa tattaatttt acggcagcaa ccgtagccga caggatgtta
     8641 gcttatgttg ttgatttact gataaaagga gcttacgtgg tcgtggtgtg gggtatcttt
     8701 ttttatgcct taggcatcaa ccaaggcatg accgggttag acgattggtc gtattacggc
     8761 ttagtggtct tgttttattc tcccgttatt ttctattcgc tcacgtttga aagtttatta
     8821 gaagggcaaa ccatcggaaa gcgattaata aaaatcaaag tggtcaagat tgatggctac
     8881 caagcctcct ttggcgatta tttgatgcgg tggttgtttc gaatcattga tgtcaattta
     8941 agtagtggag ttgttggatt aattgccatg atcaccagca gcaaaacgca acgtttgggc
     9001 gatatgactg cgggaacggc agtaatcagt ttaaaaaaca acatcaacat caaccatacc
     9061 attttagaag aaatcgacca ggcctatgtg cccacttatc cgttagtaat aaaattaagt
     9121 gataatgatg ccagaatcat taaagaaacc tttacggtag cacatatcaa acgagacttt
     9181 gccaccctaa tcaaactgcg aacaaaaatt gaagaagtca ccggcataaa gaacatttca
     9241 ggcaatgaca ccgactttat ccgaaccgtt ttaaaagatt ataattatta cacccaaaac
     9301 atgtaaatga caaggcaata tcaatttttt ctaatccacc cacctcaatg tttcacctgc
     9361 tcgatattat aggaaccata gcatttgcca tttcaggcgt gctcacagct tttaacaaaa
     9421 aactagaccc gtttggcgtc tacatcattg cgtttgtaac cgcagtgggc ggaggaacac
     9481 ttcgcgatgt gctcatcggg cgaactcccg tggtttggat gcaagacatc acctatgtct
     9541 acatgatcac catcgggttt tttatcgcct tggtttttcg taaaaagctc gacaaactcc
     9601 gcacctcgct ctttttgttt gacaccatcg ggttaggggt tttcacatta attgggttag
     9661 aaagaggcat cgaagcagga ttacatccca tcatttgcat tgccttgggc accatgaccg
     9721 cttgttttgg cggagtcatt cgcgatatac tgtgcaatga aatccccatc attttccgaa
     9781 gagaaattta tgccaccatt tgcatcatcg gaggcttgct gttttttgcc ttaaagcatt
     9841 tcaacttaaa caacgatgtg ttatacttgg tcacttccat cgttatcatc accattcgat
     9901 tgatggcggt taagtttaaa tggtatttgc ccacgatggg gagggtgtag atatctggtg
     9961 ttagggggag cgttgtgctg ataagaagaa tttgc
//
LOCUS       DAOTCW010000079         9994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_4483_length_9994_cov_41.595935, whole genome shotgun sequence.
ACCESSION   DAOTCW010000079 DAOTCW010000000
VERSION     DAOTCW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4483_length_9994_cov_41.595935"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2473)
                     /gene="uvrA"
                     /locus_tag="P5335_07545"
     CDS             complement(<1..2473)
                     /gene="uvrA"
                     /locus_tag="P5335_07545"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRZ74767.1"
                     /translation="MLHSNEMIEVLGARVHNLKNIDVSIPREKLVVITGLSGSGKSSL
                     AFDTIYAEGQRRYIETFSAYARQFLGGLERPDVDKIDGLSPVIAIEQKTTSKSPRSTV
                     GTITEIYDFLRLLFARAADAYSYNTGEKMVSYSDEQIKALILDSFKGRRINILAPIIR
                     SRKGHYAELFQQIAKQGFVKVRVDGEIQDIISGMKIDRYKTHDIEIVIDRMQIENDVE
                     TDNRLAESIKTAMHHGDDVIMVIEQESKEVRYFSRTLMCPTSGISYPNPEPNNFSFNS
                     PKGACEHCNGLGTINQINLKKIIPNPKSSIKQGGFAPLGEFKSTWIFKQLETIGEKYG
                     FKITDPIETISEEAMDMILNGGKDKFTVASKTLGITKEYKIEFEGISHFIKNQHDESN
                     SASIKRWAKEFMDEIECPHCEGSRLKKEALYFKINDKNITDLSQMDISELTKWFAVLD
                     SYLSDKQKAIAVEINKEIKSRLQFLLDVGLNYLSLNRSSKSLSGGEAQRIRLATQIGS
                     QLVGVLYILDEPSIGLHQRDNERLINSLENLRDIGNSVLVVEHDKEMIERADWVIDIG
                     PKAGRFGGQIISEGTPKQLLLEKTMTAQYLNGNLKIEVPKKRRDGNGKFLKLSGAMGN
                     NLKNVTVEFPLGKLICVTGVSGSGKSTLINETLYPILNEHFFNAVKKPQPYKKIEGLE
                     HIDKVIDIDQSPIGRTPRSNPATYTDVFSEIRSLYTQTPEAMIRGYKAGRFSFNVSGG
                     RCETCEGSGVRTIEMSFLPDVYVECETCMGKRFNRETLEIRYKGKSISDVLNMTVDEA
                     VDFFENIPKIFRKVKTIQEVGLGYIT"
     gene            2587..3108
                     /locus_tag="P5335_07550"
     CDS             2587..3108
                     /locus_tag="P5335_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74768.1"
                     /translation="MKILGIGSRIKHAEFGFGVVTNVTSKHYWVTFIEKGLETIDIDC
                     EFEVIEALEDEVDSVSFYDVERTLKSLLQKWSDVSQIVPIADKWKGGKLIMEPGTPTQ
                     NKEVPIDTFFHKIIMVRDRIRVMEQKINASNLDEAEKIDLQQYITRIYGSLTTFNVLF
                     KSTNDYFVGEKSK"
     gene            complement(3217..5034)
                     /locus_tag="P5335_07555"
     CDS             complement(3217..5034)
                     /locus_tag="P5335_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRZ74769.1"
                     /translation="MPTQNKSVLKTFFRKNFRRLEGFILYLKSYLSPRQFIYLSCFLV
                     GISASLAVIVLKTFAHWVYIFAQYIDSIFHLPYSNSTLPIIGIVLTAWVVKRVLDGSL
                     EKGTSQIMYAVAKKSGIIPKKQMYAQIITSGLTVGMGGSAGLESPITITGAAFGSNFA
                     QNYRLSYKDRTLLLACGVAAGIAAAFNAPIAGVLFAIEVVLVDVSITAFIPIMISAAT
                     GALVSTILLNEDILLSFNKQLDFDYHNLPYYIILGLASGFVSINYARTFRKIEHFFSR
                     IKFGPMRKALFGACILAILIFFFPTLFGEGYNSIKILSTNNPEQILDNTVLQGFKDNA
                     WMLLLFVGATMLIKVYATSITIGSGGNGGNFAPSLFVGSYLGFFVASFFNLLGFVKLP
                     VSNFTMVGMAGILSGLFHAPLTAIFLIAEITSGYSLMVPLMLVSSISFAISKRYEPYS
                     FDIKNLADKGDVFTDDRDRNILSGIIISRLIQSPIQTLKLAPSLEELVEKISGYNQMI
                     FPVVNDKNELLGIIDFEEIKSHLFNPYKIKYTQIKDIMRAPKEMVNYDETAETIVEKF
                     ERAQTDYLPVLKNKEVIGIISKNALLEAYREKLKNMIIE"
     gene            complement(5068..6753)
                     /gene="ggt"
                     /locus_tag="P5335_07560"
     CDS             complement(5068..6753)
                     /gene="ggt"
                     /locus_tag="P5335_07560"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRZ74770.1"
                     /translation="MKKYSLLLLILFLSCKTEKNTVLPIGLVTSKAMVVSAREEASQI
                     GVQIMEEGGNAFDAMIATELALAVSYPYAGNIGGGGFMVYRKYNGETGSLDYREKAPL
                     AATKYMYLDEKGNVIKGLSTETGLAIGVPGTIAGVFEVHKKYGTLPMETILKPVIDLA
                     RNGVLVTQKQEERLNKYRKAIVKANGSETLFSYPFQTNDTIKYEALANTLERIAKNGR
                     DEFYKGETAQKLVNFLQAKGSIITLEDLASYEVKWRDPIVNSYRDYKIISMAPPSSGG
                     ITLGQILKMIEPFDLAKMGHNSEQYIQVIVEAERRAYADRNHFLGDPDFVKVPVNELL
                     DETYLKDRMATFSFEKATKSDDVSHGEIIFSESTETTHYSIIDPFGNAVSATTTINDA
                     YGSKLYCDELGFFLNNEMDDFSSKQGVPNMFGLVGSDANSIAPQKRMLSSMTPTIVEK
                     NGDLFMVVGTPGGSTIITSVLQTILNVTEFNMSIQEAVDAPRFHHQWLPDEVIFEPNC
                     FSAEILQILKAKGYLINEKQTPVLGKVDAILVLPNTKLEGGADKRGDDKAIGF"
     gene            complement(6750..7334)
                     /locus_tag="P5335_07565"
     CDS             complement(6750..7334)
                     /locus_tag="P5335_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HRZ74771.1"
                     /translation="MNFLAHIYLSGDDDLVKIGNFMADSIRGQSYNDYSPYIKKGILL
                     HRAIDTFTDAHPIFRKSKHRLHEKYGHYSGVIIDIFYDHFLAKNWKKYSAISLEETAS
                     AFYENLQINYDLLTEPIKKMMPYMIARNWLVSYATIEGLAMILFQMDHRTKNRVAMHE
                     SIVELKEFYSEFEDEFTQFFEEIRLHCKEKIATL"
     gene            7495..8886
                     /gene="glmM"
                     /locus_tag="P5335_07570"
     CDS             7495..8886
                     /gene="glmM"
                     /locus_tag="P5335_07570"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRZ74772.1"
                     /translation="MTLIKSISGIRGTIGGKVGDNLTPVDAVKFASAYGTWLKNANAK
                     EKLKVVVGRDARISGPMIHNLVVNTLIGLGIDVIDLGLSTTPTVEVAVPLEMADGGII
                     LTASHNPKQWNALKLLNEKGEFLNGAEGAKILEIADSEAFNFSDVDSLGEITINDAYM
                     DIHIDEVLNLPLVDADAVAKRKFKVVVDGVNSSGGIVIPNLLEQMGVEVVKLYCEPNG
                     HFPHNPEPLKEHLGDICALVLKEKADFGIVVDPDVDRLAFISNDGEMFGEEYTLVAVA
                     DYVLSKTPGNTVSNMSSSRALRDITEKHKGSYQASAVGEVNVVELMKSTQAIIGGEGN
                     GGIIYPELHYGRDSLVGVALFLTHLANLDVTVAELRASYPQYYMSKNKIELTPEIDVD
                     AILVAMTDKYKNENISTIDGVKIDFATEWVHLRKSNTEPIIRIYTEAPTQQEADKLAL
                     RIIDEIKEVVNQK"
     gene            8888..9535
                     /gene="def"
                     /locus_tag="P5335_07575"
     CDS             8888..9535
                     /gene="def"
                     /locus_tag="P5335_07575"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRZ74773.1"
                     /translation="MKRNGWLLIFLCFSTLTFAQKFSKDERKLITASDTTVMFKIIQV
                     TDSSVFKILKAISEDINPKDKYIPIIAKRMYLAMQNPINPGVGIAAPQIGINRNLIWV
                     QRFDKPNEPFEFYINPKIIWRSTLLQKGMEGCLSIPNELGDVYRNHTIMITYFDRKGK
                     MNEEIIEGFTAVIFQHEMDHLNGILFTDRLIEQSQWNDSQINNEVNLFLKTKLKR"
     gene            complement(9550..9963)
                     /locus_tag="P5335_07580"
     CDS             complement(9550..9963)
                     /locus_tag="P5335_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74774.1"
                     /translation="MKTKFLVILLFFICLDFVHAQTIQNSHYSTTAYIKNDGTIQNSH
                     YATVGYIKNDGTIQNKHYATIGYIKNDGTIQNNHYSTVGYIKNDGTVQNNHYTTLGYI
                     KADGTVQNNHYATIGYAKGVAKEWAAVVYFFFNFE"
BASE COUNT         3286 a         1685 c         1661 g         3362 t
ORIGIN      
        1 atgtgatata gcctaaacca acttcttgaa tagtttttac ttttcggaat attttaggta
       61 tgttttcaaa aaagtctacc gcttcatcaa cagtcatatt caatacatct gaaattgatt
      121 ttcctttgta tcgaatttct aaggtttcac gattgaatcg tttacccata caggtttcgc
      181 attctacata aacatccggt aaaaaactca tttcaatagt tcgaacaccc gaaccttcac
      241 atgtttcgca tcgacctccg gaaacattga agctgaatcg accagcctta tagccacgaa
      301 tcatagcttc tggagtttgt gtatataaac ttctgatttc tgaaaaaaca tcagtatagg
      361 tggccggatt acttctggga gttctaccaa tcggactttg atcgatatca attaccttat
      421 caatgtgctc taaaccttct atctttttgt atggttgtgg tttttttacg gcattaaaga
      481 agtgctcatt tagaatagga tagagggttt catttattaa agttgatttt ccgcttccgg
      541 aaactcctgt tacacaaatt aatttcccca acggaaattc tacagtcaca ttttttaaat
      601 tatttcccat agcacctgat aatttcaaaa actttccatt gccatctcgt cgttttttag
      661 gcacttcaat ttttaaattg ccatttaaat attgagcagt cattgttttt tctaacaata
      721 actgttttgg ggttccttca ctaataattt gaccaccaaa tcgtcctgct tttggaccaa
      781 tatcaataac ccaatcggct cgttcaatca tttctttatc atgttctaca accaaaacgg
      841 agttgccaat gtcacgaaga ttttccaaag agttaatcaa tcgttcgtta tctcgttgat
      901 gcaatcctat gctgggttca tctaaaatat ataagacacc tactaattga gaaccaattt
      961 gggtagccaa tcggatacgt tgagcttcgc cacctgaaag cgattttgag cttctgttca
     1021 gtgataaata attcaaacca acatctaata gaaattgtaa tcgggattta atttctttat
     1081 ttatttcaac tgcaatagcc ttttgtttat cagataaata ggaatctaaa actgcaaacc
     1141 attttgtcaa ttccgaaata tccatttgtg ataaatcggt aatattttta tcgtttattt
     1201 taaagtaaag agcttccttt tttaatcgag aaccttcaca atgtggacat tcaatttcat
     1261 ccatgaattc tttagcccat cgtttaatgg aagcagaatt gctttcgtcg tgttgatttt
     1321 tgataaaatg tgaaatgcct tcaaattcaa ttttatattc tttagtaata cctaacgttt
     1381 tagaagcaac agtaaattta tcttttccac cattcaaaat catatccatt gcttcttctg
     1441 aaatcgtttc aattgggtcc gttattttaa agccatattt ttctccaata gtttctaatt
     1501 gtttaaaaat ccaggtactt ttaaattctc ccaatggagc aaaaccacct tgtttgattg
     1561 atgattttgg attaggaatt atttttttta aatttatctg attaatggtt cctaatccat
     1621 tgcaatgttc acaagcacct tttggggagt tgaatgaaaa attatttggt tccggatttg
     1681 gataagatat tcctgaagtt ggacacatta aagttctact aaagtacctt acctctttac
     1741 tttcctgttc aattaccatt atgacatcat caccgtggtg catcgccgtt ttaatacttt
     1801 cagctaagcg attatctgtt tcaacatcat tttcaatttg cattcggtca ataacgattt
     1861 caatatcatg ggttttatat cggtcgattt tcattccgga gattatatcc tgtatttctc
     1921 catcaacacg aacttttaca aaaccttgtt tggcaatttg ttgaaacaac tccgcataat
     1981 gaccttttct tgaccggatg ataggagcta atatattgat tcgtcttcct ttgaaagaat
     2041 cgagaattaa tgcttttatt tgttcgtcgg aatacgaaac catcttttct cctgtattat
     2101 agctataagc atctgccgcc cgtgcaaaca ataaacggag aaaatcataa atctctgtga
     2161 tagtgccaac agtggaacgc ggacttttac ttgtagtttt ttgctcaata gcaatcacgg
     2221 gtgaaagtcc atcaatttta tcaacgtccg gtctttccaa tccacctaaa aactgtcgtg
     2281 cataggctga aaaagtttcg atgtatcgcc gttgaccttc tgcataaatt gtatcaaacg
     2341 ccaaagacga ttttcctgaa cctgaaagtc cggtaatcac gaccagtttc tctctgggaa
     2401 tagaaacatc gatgttcttt agattgtgaa cacgagcacc taatacttca atcatttcat
     2461 ttgaatgcaa catacttttt ttggcaaacc gcaaagttac aattttgaaa gcaagattta
     2521 atcatacact tgcgaagaat ttgttaaaca atttctattt ttgaaaaaaa aataaaaact
     2581 gaatatatga aaatattagg aataggctct cgaattaaac atgcagaatt tggatttggt
     2641 gtggtaacca acgtaacgtc caagcattat tgggtaactt ttattgaaaa aggattagaa
     2701 accattgata tcgactgtga atttgaagtt attgaagcgt tggaagatga agtagattca
     2761 gttagttttt atgatgtaga acgaacgttg aaaagtttat tacaaaaatg gtcagatgtt
     2821 tctcaaattg ttcccattgc agataaatgg aaaggcggaa aactcataat ggaacctgga
     2881 actccaacgc aaaataaaga agtgccaatc gatactttct tccataaaat tattatggtt
     2941 cgcgatagaa ttagagtaat ggaacaaaaa ataaacgcca gcaatttaga tgaagctgaa
     3001 aaaattgatt tgcaacaata catcactcga atttatggta gtttgacaac ttttaatgtt
     3061 ttgtttaaaa gtacaaatga ttattttgta ggggagaaga gtaagtagtt ttattttttc
     3121 agcgttttga ctctgaatga atgttacttt aaattcattt ttaatgctaa tatattgatt
     3181 tacttttatc ttcaaattta catctgaagt ggtaacttat tcaataatca tgttttttag
     3241 tttttcccga taagcttcta ataaagcatt tttagaaata ataccgatga cttctttatt
     3301 tttaagaaca ggtaaataat ccgtttgagc acgttcaaat ttttctacga tggtttctgc
     3361 ggtttcatca tagttcacca tttctttagg agctctcatg atatctttta tttgggtata
     3421 ttttatttta taaggattaa acaaatggga tttaatttct tcaaaatcaa ttatccctag
     3481 taattcattt ttatcgttta caaccgggaa aatcatttgg ttgtaaccgg aaattttttc
     3541 aactaattct tctaaggatg gggcaagttt taaagtttgt atcgggcttt gaattaatcg
     3601 cgaaattata attccggata aaatattccg atctctatcg tcagtaaaca catcgccttt
     3661 atccgctaag tttttaatat caaaggaata aggttcatat cgttttgaaa tagcaaagct
     3721 aatggatgaa acaagcatca aaggaaccat taaactatag ccgctggtaa tttcagctat
     3781 caaaaatatg gcagttaagg gtgcatgaaa taagccactt aaaattccgg ccattccaac
     3841 catggtaaaa ttactaacag gaagttttac aaatccaagt agattaaaaa atgatgcaac
     3901 aaaaaaacct aaatatgaac ctacaaaaag ggaaggggca aaatttcctc catttcctcc
     3961 gctaccaatg gtaatactag tagcataaac ttttatgagc attgtagctc caacaaaaag
     4021 caataacatc caagcattat ctttaaaacc ttgtaaaaca gtattgtcta aaatttgttc
     4081 cggattattc gttgaaagta tttttatgct gttataccct tctccaaata aggtcggaaa
     4141 aaagaaaatt aaaatagcta aaatacaagc tccaaaaaga gcttttctca tggggccaaa
     4201 ttttattcgg ctaaaaaaat gttctatttt acgaaaagtt cgtgcataat taatggaaac
     4261 aaaaccggat gctagcccta aaattatgta ataaggaaga ttatgataat caaaatccaa
     4321 ttgtttgtta aaagacaata aaatatcttc atttaaaagg attgtagaaa ccaaagcacc
     4381 ggttgccgca gaaatcataa taggaataaa ggcagtaata ctcacatcaa ccaaaactac
     4441 ttcaatggca aaaagtacac ctgcaatagg ggcattaaat gctgctgcaa ttccggcagc
     4501 aactccacaa gctaagagta atgttctgtc tttgtaactt aagcgataat tttgagcaaa
     4561 gttacttcca aaggcagcac ctgtaattgt aataggagat tctaatccgg cagagccacc
     4621 cattcctact gttaaaccac tagtgattat ttgagcatac atttgctttt taggaatgat
     4681 acccgacttt tttgctacgg catacattat ttgtgacgtt cctttttcta atgatccatc
     4741 caaaaccctc ttaacaaccc atgcagttaa cactattcca ataataggaa gtgtactatt
     4801 actgtaaggt aaatgaaaaa tgctatcaat atattgagca aaaatataaa cccaatgggc
     4861 aaaagttttt aaaacaatta cagctaaaga tgctgaaata cctactaaaa aacaggataa
     4921 atagataaat tgcctgggag aaagatagga ttttaaatat aaaataaatc cctccaatct
     4981 tctgaagttt tttcggaaga atgtttttag aaccgattta ttttgtgtgg gcatgtgatt
     5041 tttttgaact gctaagataa aacttattta aaaaccaata gctttatcat cacctctttt
     5101 gtccgcacca ccttctaatt tagtattagg taaaactaat atagcatcaa cttttcccaa
     5161 tacaggagtt tgtttttcat taattagata tccttttgcc tttaggattt gaagtatttc
     5221 tgctgaaaaa caatttggtt caaaaataac ttcatccggt aaccattgat ggtgaaaacg
     5281 tggagcatca acagcttcct gaatactcat attgaattca gtgacattta aaatagtttg
     5341 taaaaccgaa gtgataatcg tagatcctcc cggagttccc acgaccataa aaaggtctcc
     5401 gtttttttct acaatagttg gtgtcatcga acttaacata cgtttttgag gggcaatact
     5461 attggcatca gaacctacca atccaaacat attaggaact ccttgtttag aagaaaaatc
     5521 atccatttca ttattgagaa aaaaacctaa ttcgtcacaa tacaatttgg agccgtaagc
     5581 atcattaatg gttgttgtag ccgaaactgc atttccaaag gggtcaataa tagaatagtg
     5641 agtggtttcg gtactctctg aaaagataat ttcaccatgt gaaacatcat ccgattttgt
     5701 tgctttttca aaggagaaag tagccattcg gtcctttaaa tacgtttcat ctaagagctc
     5761 attaactggg acttttacaa aatcaggatc acctaaaaaa tggtttctgt ctgcataagc
     5821 tcttcgttcc gcttctacaa ttacttgaat gtattgttca gagttatgtc ccattttagc
     5881 taaatcaaac ggttcaatca tttttaaaat ttgtcctaaa gttattccgc cgctacttgg
     5941 tggtgccatt gaaatgattt tataatcccg ataagaattt acaatcggat cacgccattt
     6001 tacttcatag gaagctaaat cttccaaggt aataatactg cctttagctt gtaaaaagtt
     6061 aactaacttt tgtgccgttt cacctttata aaattcatcc cgaccatttt tagcgatgcg
     6121 ttccaacgtg ttggctaaag cttcatattt aatcgtgtca tttgtttgaa aaggataaga
     6181 aaataaagtt tcggaaccat ttgctttaac tatcgctttt cggtatttgt ttaaacgttc
     6241 ttcttgtttt tgggtaacta aaacaccatt tctagctaaa tctatgaccg gttttagaat
     6301 ggtttccatt ggtaatgtgc catatttttt atgaacttca aaaactccgg caatggttcc
     6361 gggaacgcct attgctaatc ctgtttcggt acttaaacct ttaattacat tacctttttc
     6421 gtctaaatac atgtatttag ttgctgccaa aggggctttt tcgcgataat ccaaacttcc
     6481 ggtttctcca ttatattttc gataaaccat aaatccacca ccgccaatat ttccggcata
     6541 agggtaggaa actgctaaag ctaactctgt tgcaatcatt gcatcaaagg cattgccacc
     6601 ttcttccatg atttgtacac caatttgcga agcttcctcc cgagctgaaa ccaccattgc
     6661 ttttgaagtg accaagccaa ttggtagaac agtatttttt tctgttttgc aacttaaaaa
     6721 aaggattagt aaaagcagtg agtatttttt cataaagtgg caattttttc tttgcaatgt
     6781 agtcgaattt cttcaaagaa ctgagtaaat tcatcttcaa actcggaata aaattctttt
     6841 aattctacga ttgattcatg cattgcaacc ctatttttgg ttctatgatc catttgaaat
     6901 aaaatcatag ctaaaccttc aattgttgca taactaacta gccaatttcg ggcaatcata
     6961 tagggcatca ttttttttat tggttctgtg agtaaatcat aattgatttg taaattttcg
     7021 taaaatgctg atgcagtttc ttctaaagat atagcagaat attttttcca attttttgct
     7081 aaaaaatggt cataaaaaat atcgataatt acacccgagt aatgtccgta tttttcatgt
     7141 aatcggtgtt tgctttttct gaagatgggg tgtgcatctg taaaagtatc aattgcacga
     7201 tgcagtaata tgcctttttt aatataaggt gaataatcat tataactttg tccacgaata
     7261 ctatccgcca taaaattccc aattttgacg aggtcatcat caccggaaag ataaatatga
     7321 gccagaaaat tcatgtttct ttataatttg gtatcagtaa aaataggaaa ctttatccgt
     7381 attgaaaaat aaactacttt ttacactcta ttaaatgtta aaaaactcga taaaagggaa
     7441 agcaaaaaag ggagttcctt tttatctttg tactttaatt taaaacaaaa attaatgaca
     7501 cttataaaat caatttcggg aattcgtgga actattggtg ggaaagttgg tgataattta
     7561 actcctgtcg atgctgttaa atttgcatca gcctatggaa cttggttgaa aaatgccaat
     7621 gctaaagaaa aattaaaagt agtagttggc cgtgatgccc gaatttcagg accaatgatt
     7681 cataatttag tagttaatac attgattggt ttaggtattg atgttattga tttaggactt
     7741 tctactacgc caactgttga agttgctgtt cccttggaaa tggctgatgg cggaatcata
     7801 ttaacagctt cacacaatcc aaagcagtgg aatgctttaa aattattaaa tgagaaagga
     7861 gaatttctaa atggggcaga aggagcaaaa atattagaaa ttgcggactc tgaagctttt
     7921 aatttttctg atgtagatag tttaggagaa atcactatca atgatgctta catggatatt
     7981 cacattgatg aggtgttgaa cttgccatta gtcgatgcgg atgctgttgc taaaagaaaa
     8041 tttaaagtgg tagtggacgg tgttaattct tccggaggaa ttgtaatccc aaatttattg
     8101 gaacaaatgg gagtggaggt agtcaaatta tattgtgaac caaatggtca tttcccacat
     8161 aatccggaac cattaaagga gcatttaggg gatatctgtg ctttggtttt aaaagaaaaa
     8221 gccgatttcg gaattgtagt agatccagat gtcgaccgat tagcatttat ttctaatgac
     8281 ggagaaatgt ttggtgaaga atatacatta gtggcagttg ccgattatgt cttgagtaaa
     8341 actcccggga atacagtttc aaatatgtca tcctccagag cattacgaga cattacagaa
     8401 aaacacaaag gtagttacca agccagtgct gttggtgaag tgaatgttgt tgaattaatg
     8461 aaatctactc aggccattat tggaggtgaa ggaaatgggg gaattattta tcctgaattg
     8521 cattatggtc gtgatagttt agttggagtt gctttgttct taacccattt agcaaattta
     8581 gatgtaacag tggcggaatt gagagcttcc tatccacaat attatatgag taaaaataaa
     8641 attgaattga cacccgaaat agatgttgat gctattttag ttgctatgac ggataaatat
     8701 aaaaatgaaa atatttctac cattgatgga gttaaaattg attttgcaac agaatgggtt
     8761 cacttacgca aatcgaatac tgaaccgata attcgtattt atactgaagc accaacgcaa
     8821 caggaagccg acaaattagc tttgcgaatt attgatgaaa tcaaagaggt ggtgaatcaa
     8881 aaataagatg aaaagaaacg gttggttact tattttcttg tgcttttcaa ccttaacttt
     8941 tgctcaaaag ttttcaaaag atgaacgtaa actcattacg gcttctgata ctactgttat
     9001 gtttaaaata atacaagtaa cagattcttc tgtattcaaa attttaaaag caatttctga
     9061 agatattaat ccaaaagata aatatattcc aattatagcc aagagaatgt atttagccat
     9121 gcagaacccc attaatccgg gagttggaat tgccgcacct caaataggaa taaacagaaa
     9181 tttaatttgg gtacaacgtt ttgataaacc aaatgaacca tttgaatttt atataaatcc
     9241 aaaaattatt tggcgttcga ccttgttgca aaaagggatg gaaggatgtc tttccattcc
     9301 aaatgaactt ggtgatgtat atcgcaatca tacaatcatg attacctatt tcgacagaaa
     9361 aggaaaaatg aatgaagaaa taattgaagg ttttacagca gtaattttcc aacatgaaat
     9421 ggaccactta aacggaattt tatttacaga ccgattaatt gagcaaagtc aatggaatga
     9481 ttctcaaatt aataatgaag taaatttgtt tctgaaaact aaattaaaac gataataact
     9541 ccttttgaat tattcaaaat taaaaaagaa atacaccacg gcagcccatt cttttgctac
     9601 tccttttgca tacccgattg ttgcataatg attgttttga acagttccgt cggcttttat
     9661 atagcctaat gttgtgtagt gattgttttg aacagttccg tcatttttaa tatagccaac
     9721 agttgaatag tggttattct gaatggttcc atcatttttg atgtacccaa ttgtagcata
     9781 gtgtttattc tgaatagttc catcattttt gatatagcca acagtggcat aatgactgtt
     9841 ttgtatggtc ccatcatttt tgatataagc agttgtgctg taatgactat tttgaatagt
     9901 ttgtgcatga acaaaatcta aacaaataaa aaataatagt ataactaaga attttgtttt
     9961 cataaattta gaaacaggat tgactgtttt caaa
//
LOCUS       DAOTCW010000080         9833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_4608_length_9833_cov_42.973103, whole genome shotgun sequence.
ACCESSION   DAOTCW010000080 DAOTCW010000000
VERSION     DAOTCW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9833
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4608_length_9833_cov_42.973103"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="P5335_07585"
     CDS             <1..356
                     /locus_tag="P5335_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRZ74775.1"
                     /translation="EANMPCCSTDAKTEMTCAQGKCDLSKCATMTKDECAAMCEKNGC
                     SEECKAKCLSQYDAEGKYVGGSNEKVSKDVRVEIIKTDETAKATVTTTVNGQTETQTF
                     EGTEAEVKAKIDALK"
     gene            complement(430..1044)
                     /locus_tag="P5335_07590"
     CDS             complement(430..1044)
                     /locus_tag="P5335_07590"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRZ74776.1"
                     /translation="MHIAVAGNIGAGKTTLTRLLAKHFKWEPHFEDVVDNPYLDDFYH
                     QMERWSFNLQIYFLNSRFRQVLQIRESGKKIIQDRTIYEDSHIFAPNLHAMGLMTNRD
                     FNNYKSLFELMESMVQAPDLLIYLRSSIPNLVNQIHKRGREYENTISIDYLSRLNERY
                     EAWIQSYDKGKLLIIDVDNINFVDNPEDLGMILNRIDAELNGLF"
     gene            complement(1044..1835)
                     /locus_tag="P5335_07595"
     CDS             complement(1044..1835)
                     /locus_tag="P5335_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74777.1"
                     /translation="MKLFRICFFLLFFISVVGQEKKIIPKFVSSTTIEANEYIDSDVF
                     GFHYYIKNNVFYKQKGGELFQYKNLYLGKISKVDSQNPLKIVLFYEGFNTVVTLDNQL
                     SESQIISFSKNSTTIVASAIGLASQNRLWVFNSLTMQLGLFDLLKLTHQSLAQPFQKT
                     IQYYQADFNQFYWIEDTQLVYSCDVYGKVFSLGKVPAFTSIQFLSEKEVIYQWENKLY
                     YYNLKEDKSTLIELDKKTFQSFRFKDQFLIIFTDNEISTYQIRLP"
     gene            1965..2882
                     /locus_tag="P5335_07600"
     CDS             1965..2882
                     /locus_tag="P5335_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HRZ74778.1"
                     /translation="MKTIIVTLLTLFTAVISAFAQDFQGMAVYESKTSTADFKSNFAG
                     NRDITPEMQKTIEERMKQMFEKTFILNFDKNASIYKEEEKLDAPGQGGGMRMMSNMMG
                     GGGTHYKNVKEKIFTVDKEFFGKEFLVKDTLPKLAWKLEGETKQIGNYTCFKATAVRP
                     VSQSDFKNYRRKTEDKKESDKKEEVKVEGKTETTKTTNLTDGWDVPKEIVVTAWYTPD
                     IPINQGPENYWGLPGLILEVNEGKTIILCSKIVMNTKEKKEIKAPSKGKEVTQAEYDK
                     IVTEKMAEMREMNQGQGGGRTMQYRMGGQ"
     gene            2910..5636
                     /locus_tag="P5335_07605"
     CDS             2910..5636
                     /locus_tag="P5335_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRZ74779.1"
                     /translation="MTKKLLFLFLLTSLIAQGQSIRLEGVVQDTLRKPLEMANVMAIN
                     QETKAMDSYGITNDKGRFQLNLKPNSNYKIKISYIGFQTLDVSIETKTDNFQKAFTLK
                     EGDVNLEGVEVVHEMPVSIKGDTIIYNADSFKTGTEKKLEDVLKKLPGVEVNADGEIE
                     VEGKKVTQLLVDGKKFFEGDTKLGAKNIPSDAVDKVQVLRNYSEVSQLRGLENNNDDI
                     AINIKLKKGKNKFWFGDISAGIGPDERYIINPKLFYYSPKTSINVLSNFNNIGDVPFS
                     LRDYFRLTGGSRNTIGRNGTNFNVSSNDLGISTQRNDRAEEIDTKFGAANVTQQMSKT
                     WNLSGFAVISSDKTKMNTQSRNEYYEPNSTEVETKENRSDISNLTNNLAIFKIGSKYK
                     PSASFQFDYDISLKRSNQEETNLVETTSITNNVETFNSINSFKKQDPLSLNQSLNIFW
                     TQNEKSTWAFEVQHLYQDEDPLYNPKLVNNPFPFLVGFDDNQSFYNINQTRFVKTNRI
                     DAKADYYYSLTTKSNLNITVGNTYSYQSYNSSIFQILDNGSSLTLEDEAYKNDVRYSF
                     NDAYLGAHYKFITGKFTFNPGISFHQYTTFNEQLETKVKDDFSRVLPDVFAQYQLKKS
                     ESLTYTYRMNNVFSDINSFAQGFTFSNFNSINRGNRMLENALQQTHSLRYFKYNLFNY
                     TTIFGNLNYVKTTDPVINRAFFNGIYQTSEKMNADFENESINGMVGYQRSFLKYYKGN
                     IGLNMNWSKFNTLRVNPSNPTNPEFDFIQTTESFSQTYRASLGTQFKTWPNLELGYTV
                     TLNDYQGTTFKTQQPFAKLDYFFLDAFSFNADYDYYQYTNSSGTVNNTYDFLNTSLSY
                     RKKDAKMEYRVGITNLLNTKSLNDDSFNQTSFRTSEYLVQPRYVVFSLKYNL"
     gene            complement(5694..6944)
                     /gene="metK"
                     /locus_tag="P5335_07610"
     CDS             complement(5694..6944)
                     /gene="metK"
                     /locus_tag="P5335_07610"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRZ74780.1"
                     /translation="MAYLFTSESVSEGHPDKVADQISDALIDNFLAFDPESKVACETL
                     VTTGQVILAGEVKSNTYLDVQTIARDVIKKIGYTKSEYMFEANSCGVLSAIHEQSADI
                     NQGVDRASKEEQGAGDQGMMFGYATNETDNYMPLALDIAHALLIELANLRRENSEITY
                     LRPDAKSQVTLEYSDDNKPQRIDAIVISTQHDDFAPEAEMLAKIKNDLISILIPRIKA
                     KYPQYAHFFNDDITYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGK
                     DPSKVDRSAAYATRHIAKNLVAAGLCDEILVQVSYAIGVAKPTSINVNTYGTSKVNLK
                     DGEISKIVEEIFDMRPYFIEQRLKLRNPIYSETAAYGHMGRTPETVTKTFKLPNGEDK
                     TMTVELFTWEKLDFVNQVKSAFGL"
     regulatory      7302..7406
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            7479..8468
                     /locus_tag="P5335_07615"
     CDS             7479..8468
                     /locus_tag="P5335_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRZ74781.1"
                     /translation="MLKIEEELKSKILVLDGAMGTMLQRNNFSEEDFRGERFKDFPHP
                     LKGNNDLLSITQPEAVKAVHRAYFQAGADIVETNTFSGTTIGMADYYLEDLVYELNFQ
                     SAKIAREVADEFTDKPRFVAGSIGPTNRTASMSPDVNDPGYRAVNFDDLRIAYKQQVE
                     ALIDGGCDVLLVETIFDTLNAKAALFAIEEVKEERNLEIPVMVSGTITDASGRTLSGQ
                     TVEAFLISISHIPLLSIGFNCALGADQLKPYLKRLAMNTQLNISAHPNAGLPNAFGHY
                     DQTPEEMQALIREYLQDNLVNIIGGCCGTTPEHIKLIAEVANEFKPRQILEKI"
     gene            8468..>9833
                     /locus_tag="P5335_07620"
     CDS             8468..>9833
                     /locus_tag="P5335_07620"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRZ74782.1"
                     /translation="MPESDCKKYLKLSGLEPLIVSPESNFINVGERTNVTGSRKFLRL
                     IKEEKYDEALDIARAQVEGGAQIIDVNMDEGMLDGVYAMTKFLNLIAAEPDIARVPVM
                     IDSSKWEIIEAGLKVIQGKGVVNSISLKEGEATFIHHAKLVKRYGAAVIVMAFDENGQ
                     ADTYERRIEICKRSYDILVDKVGFPAEDIIFDPNIFPVATGMEEHKLNALDFFRATKW
                     IRENLSYANISGGVSNVSFSFRGNDKVREAMHSTFLYHAIQHGMTMGIVNPEMLEIYD
                     EIDPILLEHVEDVLLNRREDATERLLELAETYKGDYKINEKSIQEWRNGSVQERLTHS
                     LVKGIDEFIEIDVEEARINATKAIEVIEINLMAGMNVVGDLFGSGKMFLPQVVKSARV
                     MKKAVAYLLPYIEEEKDSATSSSAGKVLMATVKGDVHDIGKNIVAVVLGCNNFEIIDL
                     GVMVP"
BASE COUNT         3457 a         1548 c         1735 g         3093 t
ORIGIN      
        1 atgaagcaaa tatgccttgt tgttctactg atgctaaaac tgaaatgact tgtgctcaag
       61 gtaaatgtga tttgtctaaa tgtgctacga tgaccaaaga tgaatgtgca gcgatgtgcg
      121 agaagaatgg ttgttcagag gaatgtaaag caaaatgctt gtctcaatat gatgcagaag
      181 gaaagtatgt tggtggttca aatgaaaaag tttcaaaaga tgttagggtt gaaataatca
      241 aaacagatga aacagcaaaa gcgacagtta caacaaccgt aaatggccaa actgaaacgc
      301 aaacttttga agggacagaa gcagaagtga aagcaaagat tgacgctttg aaataataca
      361 taaagttcaa gtataaaaaa tgtctcctaa ttttaggagg cattttttta ttttattaaa
      421 tatcttggtc taaaataaac cgtttagttc tgcatcaatt cggttaagaa tcattcctaa
      481 atcttccgga ttatccacaa aattgatatt atccacatcg ataattaaaa gttttccttt
      541 gtcataactt tgaatccaag cttcataacg ttcgttaagt ctgctcaaat aatcaatcga
      601 aatggtgttt tcatattctc gacctctttt gtgaatctga ttcactaaat ttggaataga
      661 acttcttaaa tagattaaca aatcaggtgc ttgaaccatg ctttccatca actcaaacaa
      721 tgatttatag ttgttaaaat ctcgatttgt cattaaaccc atagcatgta agttgggagc
      781 aaaaatatgt gaatcctcat aaattgttct gtcctgaata attttctttc cgctttcacg
      841 aatttgtaaa acttgacgaa aacgactgtt taagaaataa atctgtaagt taaatgacca
      901 acgttccatt tggtgataaa aatcgtccaa ataagggttg tcaacgacat cttcaaaatg
      961 aggttcccat ttaaaatgtt tggctaataa tcgtgttaag gtggtttttc cggctccaat
     1021 gttgcctgct actgctatgt gcattatggt aatctaattt ggtaagtact aatttcgttg
     1081 tctgtaaaaa taatcaaaaa ttggtcttta aaccgaaaac tttgaaaggt ttttttatct
     1141 aattcgatta aagtactttt gtcttcttta agattataat aatatagttt gttttcccat
     1201 tgataaatta cttctttttc agataaaaat tgaattgaag taaaagcagg tacttttcct
     1261 aagctaaaaa cttttccata aacatcacaa gaatatacaa gttgtgtatc ttcaatccaa
     1321 taaaattgat tgaaatctgc ttgataatat tgaatagttt tttgaaaagg ttgtgctaag
     1381 gattgatggg ttaattttaa caaatcaaac aaacccaatt gcatcgttaa cgaattaaat
     1441 acccaaagtc gattttgaga agctaatccg atggctgaag caacaattgt tgttgaattt
     1501 tttgaaaacg atataatttg ggattcactt aattgattat ctaaggtaac gacagtatta
     1561 aaaccttcat aaaataggac tatttttaat ggattttgcg aatctacttt actgattttt
     1621 cccaaataaa gattcttgta ctgaaataat tcaccacctt tttgtttgta gaaaacattg
     1681 tttttgatgt agtaatgaaa tccgaaaaca tcagaatcaa tatattcatt tgcctcaatc
     1741 gtagttgatg aaacaaattt tggaattatt tttttttcct gtccaacgac agaaataaaa
     1801 aaaagcagaa aaaagcagat tcgaaataac ttcataatgg ctaaagtaca attaaacttg
     1861 tgaataataa aatggaagtt ggaattctat tttaacaaaa cattggtaac aaaaatctaa
     1921 taaactcgtc atatttgttt tgtaaaacta taaaacgttt aaaaatgaaa acaattattg
     1981 tcaccttgct tacattgttc accgcggtta ttagtgcatt tgctcaggat tttcaaggaa
     2041 tggcggtgta tgaatccaaa acaagcacag ctgatttcaa gtctaatttt gccggaaatc
     2101 gtgatattac acctgagatg caaaaaacga ttgaagaacg tatgaaacaa atgtttgaaa
     2161 aaacattcat attaaatttt gacaaaaatg catctattta taaggaagaa gaaaagctag
     2221 atgctcccgg tcaaggtggt ggaatgcgaa tgatgtctaa catgatgggc ggtggcggaa
     2281 cacattataa aaacgtaaaa gaaaaaatat ttactgttga caaagaattt tttggaaaag
     2341 aatttttggt gaaagatact ttgccaaaat tagcatggaa attagaaggt gaaacaaaac
     2401 aaattggaaa ttatacttgt tttaaggcca cagctgttcg acccgtaagt cagtctgatt
     2461 ttaaaaatta cagaagaaaa acagaggata aaaaggaaag cgataaaaaa gaagaagtta
     2521 aagtggaagg taaaacagaa actacaaaaa ccacaaattt gactgatggt tgggatgtgc
     2581 cgaaagaaat tgttgttaca gcttggtaca ctccggatat tccaattaac caagggcctg
     2641 aaaattattg gggcttaccc ggtttaattt tagaggttaa tgaagggaaa actattattt
     2701 tatgttcaaa aattgtgatg aataccaaag agaaaaaaga gataaaagct ccctcaaagg
     2761 ggaaagaagt aactcaagct gaatatgata aaattgtaac cgagaaaatg gcagaaatgc
     2821 gtgaaatgaa tcaaggacaa ggaggaggaa gaactatgca atatagaatg ggcggacaat
     2881 aatttaaatc aaattcaatt tttttaggaa tgactaaaaa actacttttt ctttttttac
     2941 ttacttcatt aatagctcaa ggtcaatcca ttcgattaga aggtgttgtt caggatacat
     3001 tgcgaaaacc gcttgaaatg gctaatgtaa tggctattaa tcaagaaacg aaagccatgg
     3061 attcgtatgg aataaccaac gataaaggac gatttcaact caatctaaag ccgaattcta
     3121 attataaaat aaaaatcagc tacattggtt ttcaaacctt agatgtttca attgaaacaa
     3181 aaaccgataa ttttcaaaag gcatttactt taaaagaagg agatgtaaat ttagaaggtg
     3241 ttgaagtggt tcatgaaatg ccggtttcaa tcaaaggaga taccattatt tataatgctg
     3301 attcttttaa aaccggaact gaaaaaaaat tagaagatgt actgaaaaaa ctcccggggg
     3361 ttgaagtaaa cgctgacggt gaaattgagg tagaaggtaa aaaagtaacg caattactcg
     3421 ttgatgggaa aaagtttttt gaaggggaca caaagttagg agccaaaaat attccgtctg
     3481 atgctgtgga taaagtacag gttttacgaa attattctga agttagccag ttgcgtggat
     3541 tagaaaataa caacgatgac atcgcaatca atataaaact taaaaaaggt aaaaataaat
     3601 tttggtttgg tgatatttct gctgggattg gtcctgatga gcgttatata atcaatccaa
     3661 aattgtttta ttacagtccg aaaacaagta tcaatgtgtt gagtaatttt aataatattg
     3721 gcgatgtacc tttttcatta cgtgattatt tccgattgac aggtggttct cgtaatacca
     3781 ttggtcgaaa tggaacaaat tttaatgttt cttctaatga tttaggtatt tctactcaac
     3841 gaaacgaccg ggcagaagaa attgacacaa aatttggagc tgcaaatgtc actcaacaaa
     3901 tgtctaaaac atggaattta agtggatttg ccgttatttc atctgataaa acaaagatga
     3961 atacacaatc aagaaatgag tattatgagc caaattctac cgaagtcgaa acaaaggaga
     4021 acagaagtga tatttctaat ttgactaaca atttggctat ttttaaaatc ggttccaaat
     4081 ataaacccag tgctagtttt cagtttgatt atgacatatc cttaaaacgt tccaatcaag
     4141 aagaaacaaa tttggtagaa acaacttcta ttacaaataa tgtagaaacg tttaattcga
     4201 taaattcatt caaaaaacaa gatcctttgt cgttaaatca aagtttaaat atattttgga
     4261 cacaaaacga aaaaagtact tgggcatttg aagtgcagca tttatatcag gatgaagacc
     4321 ctttgtacaa tccaaaattg gtaaataatc cgtttccatt tcttgtaggt tttgatgata
     4381 atcaaagttt ttataatatc aatcaaaccc gttttgtcaa aacaaatcga attgatgcca
     4441 aagcagatta ttattattcc cttactacta aaagcaacct aaatatcact gttggaaaca
     4501 cttattctta ccaaagttat aattcatcta tttttcaaat attagataat ggatcctcct
     4561 tgacattgga agatgaagct tataaaaatg atgttcggta cagttttaat gatgcttatc
     4621 taggagcaca ttataaattt ataaccggta aatttacctt caatccggga ataagttttc
     4681 accaatatac tacttttaat gagcaattag aaacaaaagt gaaagatgat ttttcaagag
     4741 ttttacctga tgtttttgct caatatcaat taaaaaaatc ggaatcgctg acctatacgt
     4801 atagaatgaa caatgttttt agcgatatca attcatttgc tcaagggttt actttcagta
     4861 attttaattc cattaacaga ggaaatcgta tgctggaaaa tgcattgcaa caaactcact
     4921 ccttacggta ttttaaatat aatttgttca attatacgac gattttcgga aatttaaatt
     4981 atgtaaaaac tacagatccg gttatcaata gagctttttt taacggaatt taccaaacca
     5041 gtgaaaaaat gaatgctgat ttcgaaaatg aaagcatcaa tggaatggta ggttatcagc
     5101 gttcattttt gaaatattat aaaggtaata ttggtttaaa catgaattgg tcaaaattta
     5161 atacgctacg ggtcaatcca tcaaatccaa ctaatccgga atttgatttt attcaaacaa
     5221 cagaatcatt tagtcaaact tatcgagcat cattagggac tcaatttaaa acttggccta
     5281 atctagaact aggatatacc gtaacgctta atgattacca gggaacaaca tttaaaacgc
     5341 aacaaccttt tgcaaagtta gattactttt ttcttgatgc tttttctttt aatgcggatt
     5401 atgattacta tcagtatact aattcctcag gaacagtaaa taacacctat gattttttaa
     5461 acactagttt aagttacaga aaaaaagatg ccaaaatgga atatagagtg ggtatcacga
     5521 acttgttaaa tacaaaatca ttaaacgatg acagttttaa tcaaacaagt tttagaactt
     5581 cagagtattt agtccaacca agatatgttg tgttttcttt aaaatataat ttataataaa
     5641 aaaccctgac tatataaata ttcagggttt ttctataaat aattggcttt gaattataat
     5701 ccaaaagcac ttttcacttg attgacaaaa tctaattttt cccaagtaaa caattcaaca
     5761 gtcatcgttt tatcttctcc atttggcaat ttgaatgttt tggtaactgt ttccggtgtt
     5821 cttcccatgt gaccgtaagc agcggtttca ctataaatgg gatttcttaa ttttaatcgt
     5881 tgttcaataa aatagggacg catatcaaaa atttcttcaa ctattttact gatttctcca
     5941 tcttttaaat ttacttttga tgtgccatac gtattcacat tgatagaagt tggttttgca
     6001 acgccaatgg catacgaaac ctgaactaaa atctcgtcac acaatcctgc agcaactaag
     6061 tttttagcaa tatgacgagt tgcgtaagca gcagaacggt caacttttga tgggtctttc
     6121 cctgaaaatg ctccaccacc atgagctcct ttaccgccat aagtatctac gataattttt
     6181 ctaccggtta atcctgtatc tccatgtggc ccaccaatca caaattttcc ggttggatta
     6241 atgtgatatg taatgtcatc attaaagaaa tgagcatatt gaggatattt tgcttttatt
     6301 ctcggaatca aaatcgaaat taaatcattt ttgatttttg ccaacatttc tgcttcggga
     6361 gcaaaatcat catgttgagt tgaaatgaca atagcatcaa ttcgttgagg tttgttatca
     6421 tctgaatatt ctaaagtaac ctgtgatttt gcatcgggac gtaaataagt aatttcagaa
     6481 ttttctctcc tcaaatttgc caattctatt aataaggcat gggcaatatc taacgccaaa
     6541 ggcatataat tatccgtttc attcgttgca tatccaaaca tcatcccttg atcacctgca
     6601 ccttgctctt ctttacttgc tcggtcaacg ccttgattaa tatccgctga ttgttcatgt
     6661 atagccgaaa gtactccaca agaattagct tcaaacatgt actcactttt tgtgtagcct
     6721 atttttttga ttacatctct ggcaatagtt tgaacgtcta agtaagtatt tgattttact
     6781 tctccggcaa gaattacttg tcctgttgtt accaaagttt cgcaagccac ttttgattca
     6841 gggtcgaaag ctaaaaaatt gtcgattaat gcgtctgaaa tttgatcagc aactttgtca
     6901 ggatgccctt cactcactga ttctgacgta aataaataag ccataataaa atttttatta
     6961 attaattaaa ttaaagaaaa aaatgaattg caaagaataa ctaaaggaga gtttctgctt
     7021 tagcattttt ttacgaggtt gcaatcagtt caaatttttc ctctttattt cgagtgcaaa
     7081 tgtataaaga ctatttgaat tgaaaaaaca atatttgatt tttatgattt taatatatgg
     7141 agtcatttaa tctatcaagt ttaataaaac agaaaataaa tttattaaaa tgcagtattg
     7201 tagatttata aattatttta atcaataaat ttaattattt tagaaaaaat ttcataaact
     7261 aatttttctt tatacctttg cacagttcat acacatactg attgttatca cgaaagccgg
     7321 agggactaga ccctgtgaaa gcttagcaac cctgcaactt agtagaaggt gctacattct
     7381 attcgctacg gcgaaacaga taaccacgag aaattctctc tcaatttcag ataacatcta
     7441 ttttaatacc aaatccttta atgggttaaa aatattttat gttaaaaatt gaagaagaat
     7501 taaaaagtaa aatccttgtc ctcgatggag ccatgggaac catgttacaa cgaaacaatt
     7561 tctccgaaga agatttccga ggcgaacgtt tcaaagattt cccgcatccg ctcaaaggaa
     7621 acaacgactt actatccatc acccaacccg aagccgtaaa ggcagttcat cgagcctatt
     7681 ttcaagcggg agctgatatt gtagaaacca acaccttttc cggaacaact atcggaatgg
     7741 cggattatta cctagaagat ttggtttacg aacttaattt tcaatcggcc aaaattgccc
     7801 gagaagttgc cgatgaattc accgacaaac cacgttttgt tgccggttcc atcggtccaa
     7861 ccaatcgcac cgcttcgatg agtccagatg tgaatgaccc gggttatcgt gccgtaaatt
     7921 ttgatgattt acgaattgct tataaacaac aagtggaagc tctcatcgat ggtggatgtg
     7981 atgtattatt ggtagaaact atatttgata cattaaatgc caaagcagct ctttttgcta
     8041 ttgaagaagt taaggaagag cgaaatcttg agataccggt aatggtctcc ggcacaatca
     8101 cggatgcttc aggacgtaca ctttccggtc aaaccgtcga agctttttta atttccattt
     8161 cacatattcc attgctaagc atcggattta attgtgcgtt aggtgccgat cagttgaagc
     8221 cttatttaaa acgtttggcg atgaatacgc agttaaatat ttcagctcat ccaaacgcag
     8281 gattacctaa cgcttttgga cattacgacc aaacacctga agaaatgcaa gcattaatcc
     8341 gtgaatattt acaagataat ttggtaaata ttatcggtgg ttgttgtggt acaactcccg
     8401 aacacatcaa attaattgcc gaagttgcca atgaatttaa acccagacaa attttagaaa
     8461 agatataatg cctgaatcag attgtaaaaa atatttaaaa ctttcaggat tggagccgtt
     8521 gattgtatct ccggaatcca attttattaa tgtaggtgaa cgaaccaacg tgacgggctc
     8581 tcgaaaattt cttcgcttaa tcaaggaaga gaaatacgac gaagctcttg acattgcccg
     8641 agcacaagtg gaaggtggag ctcaaattat tgatgtcaat atggacgaag gaatgttgga
     8701 tggcgtttat gctatgacca aattcttaaa tttgattgca gccgaacccg atattgctag
     8761 agttccagtg atgattgaca gttccaaatg ggaaatcatc gaagctggat taaaagtgat
     8821 tcaaggaaaa ggcgtcgtaa attccatatc gttaaaagaa ggcgaagcaa cttttatcca
     8881 tcatgccaag ttggttaaac gttacggagc cgctgtaatc gtcatggctt ttgacgaaaa
     8941 tggtcaagcg gatacgtatg aaagacgtat cgaaatctgc aaaagaagtt atgatatttt
     9001 agtagataaa gttggatttc cagcggaaga tatcattttt gacccaaata ttttcccggt
     9061 tgcaaccgga atggaggagc ataaactaaa tgcgttagat tttttcagag ccactaaatg
     9121 gattcgggaa aatctttcgt atgccaatat ttcaggtgga gtaagtaacg tttcattctc
     9181 ctttagaggt aacgataaag tccgtgaagc catgcattct actttcctct atcatgcgat
     9241 tcaacatggc atgactatgg gaatagtaaa tccggaaatg cttgagattt atgatgaaat
     9301 tgatcctatt ttattagaac atgttgaaga tgttctttta aatagacggg aagatgctac
     9361 tgaacgactt ttagaattag ccgaaacgta taaaggtgat tataaaatca atgaaaaatc
     9421 aattcaagaa tggcgaaatg gctcagttca ggaacgatta acccattcat tagtaaaagg
     9481 aatcgatgaa ttcatcgaaa tagatgtcga ggaagcaaga ataaatgcta caaaagcgat
     9541 tgaagttatc gaaatcaatt taatggcagg aatgaatgtg gtgggagatt tattcgggag
     9601 tggaaaaatg tttttgccac aagttgtaaa atctgctaga gtgatgaaaa aagcagtggc
     9661 ttatttgctt ccgtatattg aagaagaaaa agattcagca acttcatcct cagcaggcaa
     9721 agtcttaatg gcaacggtaa aaggtgatgt gcacgacatt gggaaaaata ttgtagcagt
     9781 cgttttagga tgtaacaatt ttgaaatcat tgatttaggg gttatggttc ctc
//
LOCUS       DAOTCW010000081         9820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_4621_length_9820_cov_44.661444, whole genome shotgun sequence.
ACCESSION   DAOTCW010000081 DAOTCW010000000
VERSION     DAOTCW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4621_length_9820_cov_44.661444"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1217
                     /locus_tag="P5335_07625"
     CDS             276..1217
                     /locus_tag="P5335_07625"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRZ74783.1"
                     /translation="MSHLEPEAKIFACSKSVYLAEQIATHYGVPLGKVTFSQYSDGEF
                     QPSFEESIRGLRVFIVCSTFPSADNLMELLLMIDAAKRASARHITPVMPYFGWARQDR
                     KDKPRVPIGAKLVAKLLETAGATRIMTMDLHADQIQGFFEKPVDHLFASTIFLPYVKS
                     LNLENLTIASPDMGGSKRAYAYSKFLESDVVICYKQRKIANVIDTMELIGEVKGRNVV
                     LVDDMIDTGGTLAKAADLMIEKGALSVRAICTHAILSGDAYEKIEKSKLSELIVTDSI
                     PLKKESNKIRVVSCAPLFAEVMHMVQHNNSISGKFLM"
     gene            1324..1932
                     /locus_tag="P5335_07630"
     CDS             1324..1932
                     /locus_tag="P5335_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HRZ74784.1"
                     /translation="MKSITIKGSERESVGKKATKAVRDAGMVPCVIYGGDQPVHFAAE
                     EKAFKNLVYTPNAHTVVIELNNGKKADVILQDIQFHPVSDKILHIDFYALKDDKEITM
                     EIPVKVTGTSPGVLSGGVLRLNQRRLKVKALPKNLPDFIEANISELEMGNKLYVTKVV
                     TNNFKLMHPDNTVVAQVRISRAAMKAAQEAAKAEKGGAKKKK"
     gene            2065..2688
                     /gene="pth"
                     /locus_tag="P5335_07635"
     CDS             2065..2688
                     /gene="pth"
                     /locus_tag="P5335_07635"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRZ74785.1"
                     /translation="MIKWISKLFQSNKTVSKPDMKKFLIVGLGNSGAEYVNTRHNIGF
                     KVLDHLAKKESLTFQTAKLGDIAEIRIKGRTLLLLKPNTFMNLSGKAVKYWMEKENIE
                     KENILVITDDLNLSFGTIRIKAKGSDGGHNGLKSIQSLLATTEYARFRFGISDEFKKG
                     KQVDYVLGEWNEDEKAKLPERLDKSIEIVYSFALAGLNNTMNEFNGK"
     gene            2694..3203
                     /locus_tag="P5335_07640"
     CDS             2694..3203
                     /locus_tag="P5335_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74786.1"
                     /translation="MKKAVVFGLIAFILSCKAQLPSKEELTLTSDCPKDGTCQIELHK
                     NKSLVVIYDNFGGMYYELKETAGKSVIHFQYNKTTEANLQDGHYREDILLEINNNTKA
                     LNLENTELQQTKLIFGRHCFCKGQTGYFKVENGKLTIQKVEKKGVLKIDFSTSKIPQI
                     IRSVEANIN"
     gene            complement(3300..6557)
                     /locus_tag="P5335_07645"
     CDS             complement(3300..6557)
                     /locus_tag="P5335_07645"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HRZ74787.1"
                     /translation="MKKLFLFLFLSLFFQIDAQNDKLWKSLPNDLSVHVSKNTQRESF
                     PENFKLFQLNRTLFTQQLSTAVNRFSGQKGIEITLPNTAGNLERFEVFEASNFSEELQ
                     TQFPFIRSYFGKGIDDKTAQTRISVDPNGIQVMTFRADKKTEFMESYSADGLIYAVYT
                     SSRTKGKLPFTCSTEDHKLVLDLNRSAQQPQSNTGELLIFRLALSCNGEYTQYFGGTV
                     AGALAAMNATMTRVNGVFEKDFSIHMDLIPNNTSVIYTNAANDPYTTMGSWNGQLQTT
                     LTTVIGEANYDVGHMFGATGGGGNAGCIGCVCVDGQKGSGITSPADGVPAGDTFDIDY
                     VAHELGHQFGANHTFSHNVEGSGVNVEPGSGSTIMGYAGITTRDVQQNSDDYFVYASI
                     KQVQDNMVGKTCPTRITLTNAAPTVNAGADYVIPKSTPFVLSGSAVDPNGDVLTYCWE
                     QNDSATTQTGANSAASATKTGGPNWRSYTPVSSPDRYCPPLTRVVANQSTTQGSEIVV
                     EALNSVARTMNFVLTSRDNFSGAGQTGSDAMVVTVNAVAGPFLVTNPNTAATWPAGSN
                     QSVTWDVAGTTANGVNAAYVDIYLSTDGGFNYPYLLASQVPNDGSEVITVPNQPGMTN
                     RIMVKGNNHIFYDISNANFSISSAPTTFSVAFNGIVGEQNKSNCTGADVSYTIPYSTL
                     GGFSGTTTFSVTGQPAGSTATFSPASTNADSNVVLTISDTQNATPGFYSMTVTAVSGA
                     ETKTVPLYFQLFDSNFNGLNLNSPANFATGQPVNVTLTWTAIANATAYDVQVASDMMF
                     TNIIASTTVFTNSYTTNGLAEVTDYYWRVLPKNTSCSGVYTEAFTFGTGQVVCSNFVS
                     TNVPITIPTTANVTVNSTLVVPDTLLLSDVNVTVNIAHTWVNDMTITLISPAGTQVQL
                     VANPCVSDNLQNIQATFDDSGNTVVCATNPAISGIVKSVQPLSAFNGQSATGTWTLRV
                     LDSFNQDGGAINSWSLNLCSTQQPLSVQEYEQLNVAVYPNPSNGNFNVQYTSITSNPI
                     AISVYDMRGRAVYTKTYENTGLINQPIALTDVQSGVYLFTVVDGNQKGVKRIVIQ"
     gene            complement(6839..9490)
                     /locus_tag="P5335_07650"
     CDS             complement(6839..9490)
                     /locus_tag="P5335_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HRZ74788.1"
                     /translation="MKKTLLLILLTAFTFTSGMAQADKFWVMTKESVSEVSKTAQRED
                     FPAVSTMYRLNTSLLKQTLMNAADRFSGNSSVVVTMPNALGEMERFQMYEASNFEPAL
                     QAQFPEIRAYIGIGLDDPKAQLRMSVDPKGIQTMTFRAGQRTEFMEPYAADGSVYAFY
                     VSTRTKGKLPFTCSTAEHSVTDRLATDKDINVVEANNLVLKTFRLALSCTGEYTTFHG
                     GNVAGALAAMNATMTRVNGVFEKDFAVRMNIIANNSAVIYTNAATDPYADSAQMNLWN
                     AQLQSTLTSVIGEANYDVGHLFGATGGGGNAGCIGCVCVDGQKGSGITSPADGVPAGD
                     TYDIDYVAHELGHQFGANHTFSHAVEGSGVNIEPGSGSTIMGYAGITNYNVQGNSDDY
                     FAYRSILQVQTNLLNKTCPVSTPLTNPALTVTSGGNWTIPQGTAFILTGTNPTNNPGA
                     TFTWEQNNNANSSVTGAQSVCFPTKSVGPNFRSVIPTATPVRYMPAFSSVLNNSLSTT
                     WESVSTIQRILNFTLTARDNIANGGQTNTASTAITVNAAVGPFTVTSQNSSTSSWAQG
                     STQTIAWNVNNTNTLVGSTEVDILLSTDGGATFSTVLAAATPNDGSQDIVVPNVLTQS
                     ARIMIRPTGNIYYALNGAFFPIGYTCSGISNSTSAAITDGLGANLAGPTTVSNIVSTE
                     SGDITSIEVTVNVTHSWIGDLVIALVHPDGTSRNLWNRTCNNPQNGNINVTFKDGAGT
                     IVCGTPTSGTYSPVQTLAPFLGKPKSGNWSLRLTDFYNGDTGTLNSWSINFGCTLGKE
                     DFSLQSLVVYPNPSKGNFNVSFDNVASESVNISVFDIRGRKIFENDYQGGSNFNQNIQ
                     LNNAQPGVYLLNVTDGERKEVKRIIVE"
     gene            9654..>9820
                     /locus_tag="P5335_07655"
     CDS             9654..>9820
                     /locus_tag="P5335_07655"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HRZ74789.1"
                     /translation="MKKFQSLFDYSSNQKSIVTLGTFDGVHIGHKKIIEKLVQNSSNC
                     ECESLVLTFFP"
BASE COUNT         3297 a         1776 c         1731 g         3016 t
ORIGIN      
        1 cacctaagta cacgagtttg agtcgtgcgc gtctaccaat tccgccatcc gggcaattta
       61 gatttttaag tttttgacta atttttataa aaaattaatt aaagacaatg ccatccgggc
      121 ataaggatgc ttatttgtgg gtgcaaatgt aatcagtttt ctttactttt tcaaaaaaat
      181 agtcgaaaat atccttccca agttgatttt tatttctaaa tttgcacctc gtttcggcga
      241 cacacaacta actaactaca taacaactat ttaaaatgtc acatttagaa ccggaagcaa
      301 aaatatttgc ttgttcaaaa agtgtatact tagcggaaca aattgccacg cattatggtg
      361 tcccgctagg aaaagttacg ttctcacaat atagtgatgg tgaatttcaa ccttcgtttg
      421 aagaatcgat tcgtggatta cgcgttttta tcgtttgttc aacctttcca agtgcggata
      481 acttaatgga attgttatta atgattgatg cggcaaaaag agcatcagcc agacatatca
      541 ctcccgttat gccttatttt ggatgggcaa gacaagaccg aaaagacaaa ccgagagtgc
      601 cgattggagc taaattagtc gctaaattat tagaaactgc cggagcaaca agaatcatga
      661 ccatggattt gcatgccgat caaattcaag gattctttga gaaaccggtt gatcacttgt
      721 tcgcctcgac catcttttta ccttatgtaa aaagtttaaa tttggagaac ctaacaatcg
      781 cttcacctga catgggtggt tccaaaagag cctatgctta ttctaaattt ttagaatcag
      841 atgtggtaat ttgctacaaa caacgaaaaa tagccaatgt aattgataca atggagttaa
      901 tcggagaagt gaaaggaaga aacgttgttt tagtagatga catgattgac accggtggaa
      961 ccttagcaaa ggcagccgat ttaatgattg aaaaaggagc tttgagtgtt cgtgcaattt
     1021 gtacacacgc cattttatcg ggcgatgcgt atgaaaaaat tgaaaagtca aaattgagtg
     1081 aattaatagt aactgattct attccgttaa agaaggaatc aaacaaaata agagtcgtga
     1141 gttgtgcgcc tctttttgcc gaagtaatgc acatggtgca acacaacaat tccattagtg
     1201 gaaaattttt aatgtaaaag cagagttggc aataggcctt ctcaattatt tataaatccg
     1261 gcatcaagcc taaagcttat tgcctagagc aaagaatttt tattaactat atatttattt
     1321 acaatgaaat cgattacgat taaaggatca gaaagagaaa gcgtaggcaa aaaggcaacg
     1381 aaggccgtac gtgatgctgg aatggttcct tgcgttattt acggaggaga tcaaccagta
     1441 cattttgcag cagaagaaaa agcgttcaaa aacttggtgt acactccaaa cgcacacact
     1501 gttgtaattg aattaaataa cggaaaaaaa gcagatgtga ttttacaaga catccaattt
     1561 cacccggtat cagacaaaat tttacacatt gacttctatg cgttaaaaga cgacaaagaa
     1621 atcacaatgg aaatccctgt gaaagttaca ggaacttctc caggtgtatt atcaggtgga
     1681 gttttacgtt tgaaccaaag aagattaaaa gtgaaagctt taccaaaaaa tctacctgat
     1741 tttatcgaag caaacatctc tgaattagaa atgggtaaca aattatatgt aacgaaagtg
     1801 gttactaata attttaaatt aatgcaccct gacaatacag tagttgctca ggtaagaatt
     1861 tctcgtgcag caatgaaagc cgctcaagaa gctgccaaag ccgaaaaagg tggagcaaaa
     1921 aagaaaaaat aattctattt tctcaatatc aaaagcatca gttcacgctg atgctttttt
     1981 tttgcattta ttttcgggat tcaaaggatg cttaaattcc ttccatccaa tttttaccat
     2041 taataatatt tgtatttttg cttcatgatt aaatggattt caaaactatt tcaaagcaac
     2101 aaaactgtct ctaaaccgga tatgaaaaaa ttcttaattg tcggactcgg aaatagtggt
     2161 gccgaatatg tcaatacacg ccacaacatc ggatttaaag tgcttgacca tttggctaaa
     2221 aaagaaagtt taacttttca aaccgccaaa ctgggtgata ttgccgaaat cagaattaaa
     2281 ggcagaacgt tactcctatt aaaaccaaat acattcatga accttagtgg taaagcggtt
     2341 aagtattgga tggaaaaaga aaatattgaa aaagaaaaca tcttagtcat tacagacgat
     2401 ttgaacttga gttttggcac gattcgaata aaagcgaaag gaagtgatgg cggtcataat
     2461 gggttgaaaa gcattcaaag tttgttggca actacagaat atgcgagatt tcgatttggc
     2521 attagcgatg aatttaaaaa aggcaaacaa gtggattatg ttttagggga atggaacgag
     2581 gatgaaaaag caaaactgcc ggagagatta gataaatcaa ttgaaatagt atattccttc
     2641 gccttagcag gacttaataa tacaatgaac gaatttaacg gaaaataatc caaatgaaaa
     2701 aagcggtggt attcgggtta attgctttta tactttcctg taaagctcaa cttccctcaa
     2761 aggaagaact tacgttaacc agtgattgcc caaaagatgg tacgtgccaa attgaactac
     2821 acaaaaacaa atcattagta gtgatatatg ataattttgg cgggatgtat tatgagttaa
     2881 aggagacggc cggaaaatct gtgattcatt ttcaatacaa caaaactaca gaagccaatt
     2941 tacaagatgg ccattaccgg gaagatattt tactagaaat caacaacaat accaaggctt
     3001 taaatctgga aaatacagaa ttacaacaaa ccaaacttat ttttggccga cactgttttt
     3061 gtaaaggaca aacaggatat tttaaagtag agaatggtaa attaactatt caaaaggttg
     3121 aaaaaaaggg agtattgaaa atagattttt ctacttccaa aatcccacaa attatccgtt
     3181 ccgttgaagc caacatcaac taaaaagccc actcaattat agactgaacc caaaagttta
     3241 gacactattt gggggcagtg tattaaggat gggctttata ttaatataaa taaatgttat
     3301 tattgaatta ctattctttt tactcctttt tgattcccat caacaacagt aaataaataa
     3361 actccggatt gaacatcagt taacgcaata ggctggttaa ttaaacctgt gttttcataa
     3421 gttttggtat aaactgctct ccctcgcata tcataaactg agatagcaat tggatttgaa
     3481 gtaatagaag tgtactgaac attaaaattc ccattacttg gattcggata aaccgccaca
     3541 tttaattgtt catattcttg taccgacaaa ggctgctgtg tgctacacaa attcaaactc
     3601 caactattta ttgccccacc atcttgatta aatgaatcta aaacgcgtaa agtccaagtt
     3661 cccgtcgctg actgaccatt gaaagcagat aatggttgaa cagacttaac aatccctgaa
     3721 atagcaggat ttgttgcaca aactactgta ttccctgaat catcgaatgt agcttgtata
     3781 ttttgtaaat tatcactaac gcatggattg gcaaccaatt gaacttgcgt tccggcaggg
     3841 ctaattaacg taatggtcat gtcatttacc catgtatgag caatgtttac agtaacatta
     3901 acatccgata ataacaaggt atccggaaca actagtgttg aattaacagt aacattagcg
     3961 gtagttggaa tagtaatagg aacgttagtt gaaacaaaat ttgaacaaac cacctgaccg
     4021 gttccaaagg taaaggcctc agtatatact ccactacaag aagtgttttt aggcaaaact
     4081 ctccaatagt aatccgtaac ctcagctaag ccattggttg tataactatt tgtaaaaaca
     4141 gttgttgagg caataatatt tgtaaacatc atgtctgatg ctacttgaac atcataagca
     4201 gtggcattag caattgcggt ccaagtaagg gttacattaa caggttgacc ggtagcaaaa
     4261 ttagccggag aattcaaatt caatccatta aagttagaat caaataattg gaagtataaa
     4321 ggaacagttt ttgtttcagc tcctgacaca gccgttactg tcatcgaata aaaaccaggc
     4381 gtagcatttt gcgtatcact aatggttaaa acaacattac tgtcagcatt tgttgaagct
     4441 ggactgaaag tagctgttga ccccgctggt tgtccggtaa cagaaaaagt agtagttccg
     4501 gaaaatccac ctaatgttga ataaggtatt gtataactca catctgcccc tgtacaattt
     4561 gatttatttt gttctccaac aataccatta aaagcaactg aaaaagtagt aggagcacta
     4621 ctgatgctga aattagcatt tgaaatatca taaaaaatgt gattattccc tttaaccatt
     4681 attcgattag tcattccggg ttggttagga actgtaatta cttcagaacc gtcattcgga
     4741 acttgactgg ctaataaata aggataattg aatccaccat cggtagataa ataaatatcg
     4801 acataagcgg cattcacacc atttgcggtg gtgcctgcta catcccaagt tacactttga
     4861 ttggaaccgg ctggccatgt tgctgctgtg ttcggattag tcactaaaaa tggtcctgca
     4921 acagcattaa ccgttacaac catagcatcg gaaccggttt gacctgcacc tgaaaaatta
     4981 tctctggagg ttaaaacaaa attcatggtt cgagcgacag aatttaacgc ttccacaacg
     5041 atttcacttc cttgcgtggt agattgatta gcaaccactc tggttaaagg tgggcaataa
     5101 cgatctgggg aagaaaccgg tgtataagaa cgccaatttg gtccacccgt tttggtagca
     5161 gaagcggcac tgtttgctcc cgtttgggtt gtagctgaat cattttgttc ccaacaataa
     5221 gttaaaacat caccattggg atctacggct gaacccgaaa gcacaaaagg agtacttttt
     5281 gggataacat aatcggcacc tgcattaaca gttggagcgg cattggtaag tgttattcta
     5341 gttggacatg tctttccaac catgttatcc tgtacttgtt tgatactcgc atagacaaaa
     5401 taatcatcag aattttgttg tacatcacgg gtagtaattc cggcataacc cataatcgta
     5461 gaaccacttc cgggctctac attcacacct gaaccttcaa cattatgcga aaaagtatgg
     5521 ttagccccaa attggtgacc tagttcatga gctacataat caatatcaaa agtatctcca
     5581 gccggcacac catctgctgg ggaagtaatt ccactccctt tttgtccatc tacacaaaca
     5641 cagcctatac aacctgcatt tccacctcca ccggttgctc caaacatatg gcctacgtca
     5701 taatttgctt ctccgattac agttgtcaaa gtagtttgca attgaccgtt ccaacttccc
     5761 atagttgtat aaggatcatt agcggcattg gtataaataa cactcgtatt atttggaatt
     5821 aaatccatat gaatggaaaa atctttttca aatactccat ttactctggt catggttgca
     5881 ttcatcgcgg caagtgcacc ggcaactgtg cctccaaaat attgtgtata ttcaccatta
     5941 caagataaag ccaatctaaa aattaatagc tctccggtgt ttgattgcgg ttgttgtgca
     6001 cttcgattta aatccaacac taatttatga tcttctgtgg aacaggtaaa aggtaatttc
     6061 ccttttgttc tggaggaagt ataaacggca taaattaatc catcggccga ataagattcc
     6121 atgaattcgg ttttcttatc cgctctgaaa gtcataactt gaattccatt cggatcaaca
     6181 ctaattctgg tttgagctgt tttatcatca attccttttc caaaataaga acgaataaaa
     6241 ggaaactgtg tttgaagttc ttctgaaaaa ttggatgctt caaatacttc aaatcgttct
     6301 aaattacccg ctgtatttgg caaagtaatc tcaatcccct tttgtccgga aaaacgatta
     6361 accgcagttg ataattgttg tgtaaataaa gttcgattta actgaaataa tttgaaattt
     6421 tcaggaaaac tttctcgttg cgtattttta gaaacgtgaa cagataaatc gttgggtaaa
     6481 gatttccata atttatcatt ttgtgcatct atttgaaaaa atagacttag aaataaaaat
     6541 aaaaataatt ttttcatgtt tagtaaaagt ttgtttggaa tccaaatgta tacaattatt
     6601 cattttttaa atgttaattt ttaaattgaa ttgaatatta actttttgca gtgaaatatt
     6661 aatttaactt tttgtcaaaa ttaaaaacaa agattaagca aaaaatattt tgcaactaat
     6721 tacattttca agctacagct aatcacaagt atctttaaat aattcaaaaa aataacgttt
     6781 aaaaataaaa aggctgtcaa aagtttgaca gcctttttat aatatataaa gaaattgatt
     6841 attctacaat aattcttttt acctcttttc tttctccatc ggttacattc aataaataaa
     6901 ctcctggttg ggcattattt aattgaatgt tttgattgaa gttacttcca ccttggtagt
     6961 cgttctcgaa tatttttctg ccacgaatat caaaaacaga tatgttaact gattcagaag
     7021 caacattatc aaaacttaca ttaaagttac ctttacttgg gtttggataa accactaatg
     7081 attgtaaact gaaatcttct tttcctaaag tacaaccaaa attgatagac caagagttta
     7141 aagttcctgt atcaccatta taaaaatcag tcaatctcaa tgaccaatta ccactttttg
     7201 gttttcctaa aaagggagct aaggtttgaa caggactata cgttcctgaa gttggtgttc
     7261 cacaaacaat agttccggca ccatctttaa atgtaacatt gatatttcca ttttgtggat
     7321 tattacaagt tctgttccaa agattacggg atgttccatc agggtgaacc aaggcaatca
     7381 ctaaatcgcc aatccaagaa tgagtaacat tcacggttac ttcaatggaa gtaatatctc
     7441 cggattcagt ggacacaata ttagatacgg tggttggacc ggctaaatta gctcctaaac
     7501 catctgtaat agcagcagaa gtactattag aaataccaga acaagtgtaa ccaattggga
     7561 aaaaagcacc atttaaagca tagtaaatat ttccggttgg tctgatcatg attctagcag
     7621 attgagtcaa tacattagga acaacaatat cttgagaacc atcatttggt gtagcagcag
     7681 ctaaaactgt tgagaaagtt gctccgccat cagttgataa taatatatct acttccgttg
     7741 aaccgactaa cgtattggtg ttgttcacat tccatgcaat tgtttgggta gatccctgtg
     7801 cccatgaaga agtactactg ttttgtgacg ttactgtaaa aggtccaact gcagcattaa
     7861 cagttattgc tgtagatgcc gtattcgttt gaccaccatt ggcaatatta tctcttgcag
     7921 ttaatgtaaa gttcaatatt ctttgtatag ttgatacaga ctcccaagtt gtgctaagtg
     7981 aattatttaa taccgagcta aatgctggca tatatcgaac aggtgtagct gttgggatga
     8041 ccgatctaaa gttaggacca acacttttag ttggaaaaca aacactttgt gctcctgtta
     8101 cagaagaatt cgcattatta ttttgttccc atgtaaatgt agccccagga ttatttgttg
     8161 ggtttgtacc agttaatata aaagctgtcc cttgaggaat agtccagttt cctccagatg
     8221 taactgttaa agctggatta gttaatggtg tggaaacagg acatgtttta tttagcaaat
     8281 ttgtttgcac ttgaagaata cttctgtaag caaaataatc atcactattg ccttgaacat
     8341 tatagtttgt aatacctgca tagcccataa ttgttgaacc actacctggt tcaatattca
     8401 caccactacc ttcaactgca tgagaaaaag tgtgattagc tccaaattgg tgacctaatt
     8461 catgagctac ataatcaata tcataggtat ctccggcagg aactccatct gcaggagaag
     8521 taattccact acctttttgt ccatcaacac aaacacaacc aatacatcct gcatttccac
     8581 caccacctgt tgctccaaat aaatgaccaa catcatagtt tgcctctcca attactgaag
     8641 ttagagtgga ttgtaattga gcattccata aattcatttg agctgaatcc gcataaggat
     8701 cagtagcagc attagtatat attacagctg agttattggc tataatattc attcgaacag
     8761 caaaatcttt ttcaaaaaca ccattcactc tagtcattgt tgcgttcatt gcggctaaag
     8821 ccccagcaac attcccacca tgaaaagtag tatattcacc ggtacaggac aaagcgagtc
     8881 taaatgtttt taatactaaa ttatttgctt ccacaacatt aatatctttg tctgtagcta
     8941 gtctatctgt tacagagtgt tctgccgttg aacaagtaaa aggtaattta ccttttgttc
     9001 tagtggacac gtaaaaagca taaactgaac cgtccgcagc ataaggctcc ataaattctg
     9061 ttctttgacc ggctctaaaa gtcatcgttt gaattccttt tgggtcaaca ctcatccgga
     9121 gttgtgcttt tggatcatct aaaccaattc caatataagc tctgatttcc ggaaattgtg
     9181 cttgtaaagc aggttcaaaa ttggaagcct catacatttg aaatcgttcc atttcaccta
     9241 gtgcatttgg catagtaacc acaacgctac tattacccga aaaacgatca gcagcattca
     9301 ttaaagtttg tttcaataaa gacgtattca aacgatacat cgttgaaaca gcgggaaaat
     9361 cttccctttg agctgttttt gatacttcag aaacagattc ttttgtcata acccagaatt
     9421 tatctgcttg tgccattccg ctggtaaacg taaaagcagt taaaaggatt aatagtaatg
     9481 tttttttcat tttagtttgt aattaattaa aagacaaata tattataatt ttaattattt
     9541 tcaatttttt ttaagatgtt gtaacaaaat aatggttttc atatcgaata ttcttattaa
     9601 ttggaaactt cttgcaaata atcacttatt gtttaaattt gtaaaaaaaa gacttgaaaa
     9661 aatttcaatc gcttttcgac tatagttcta atcagaagtc cattgtaacc cttggcactt
     9721 ttgatggggt tcatattggg cataaaaaaa taattgaaaa acttgttcaa aactcctcaa
     9781 attgtgaatg tgaaagttta gtacttactt ttttccctca
//
LOCUS       DAOTCW010000082         9697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_4710_length_9697_cov_45.714789, whole genome shotgun sequence.
ACCESSION   DAOTCW010000082 DAOTCW010000000
VERSION     DAOTCW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9697
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4710_length_9697_cov_45.714789"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="P5335_07660"
     CDS             <1..383
                     /locus_tag="P5335_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRZ74790.1"
                     /translation="QLTIPILNGFVTKTNVEKSKVAVERGKIAYEQEELNLQRNVYTA
                     FTDAKGALNSYEASLVTLEARQEAFISAKERFEVGLMNAFDYNQSQTLLVNAQSEVLR
                     TKYDYIFKTKILEFYFGIPIIQKP"
     gene            387..1718
                     /locus_tag="P5335_07665"
     CDS             387..1718
                     /locus_tag="P5335_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRZ74791.1"
                     /translation="MSKKKVYIFLGIAVVIIVSLIILSKLGILGNKNIGKEVEIATVT
                     EMTIIETVSATGKIQPETEIKISSEVSGEIIDLPIVEGQMVKKGQLLVRINPDLYTSS
                     LNRSVAGLSNTKAGLNQAEASLKEAQANYNRNKSLFEKGIISKSEWDKIVAVYEGAQA
                     AKQAAYFGVQSASATVTEARDNLGRTTIYSPSDGTISLLSVELGERVLGTQQMTGTEI
                     LRVANLNNMEVEVDVNENDIVKINIGDSTNIEVDAYLKKEFKGIVTSISNSASTAVTA
                     DQVTNFKVKVRILKESYLDLIEGKPLNYSPFRPGMTATVDIITKRKENVVAVPISAVV
                     MKSDTLGTNKVEVKQDDKNDEEGEVVKKVDKKLECVFVKVGDKAKIRIIKTGIQDDSN
                     IEVIEGLKKGDVVIIGPYTTVTKDINSGDTVFVKTEEEKAKEKEKLNKKKK"
     gene            1799..2464
                     /gene="tsaB"
                     /locus_tag="P5335_07670"
     CDS             1799..2464
                     /gene="tsaB"
                     /locus_tag="P5335_07670"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRZ74792.1"
                     /translation="MVYILNIETSTKNCSVAVACEGKVILSKEIAEIGYSHAEKLHVF
                     LEELLKETDLSFKDINAIAVSQGPGSYTGLRIGVSASKGLCYALDIPLIAVDTMQILA
                     NQVTVEEGIIIPMIDARRMEVFSAIFDKNHLKIRETKAEILEEDSFSINNGPIYIIGD
                     CASKVKTVLTHEKFVFLENHIFPSAKEMATLSYRLFKQNNFVDVAYFEPFYLKDFMLT
                     QKS"
     gene            complement(2461..3315)
                     /locus_tag="P5335_07675"
     CDS             complement(2461..3315)
                     /locus_tag="P5335_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013185988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRZ74793.1"
                     /translation="MFESFFMELITLNTDFITRYWDYFTTVLIEYSPRLISAAIIFVV
                     GWTVIKLIKKLIMRIMVKREMEPTLSKFLADILIWTLKILLFVTVISRLGVENSSFVA
                     IIGAAGLAVGLSLQGSLSNFAGGVLIIMFKPFKVGDFIEAQGVMGTVKQIQIFVTQLS
                     TVDNQIIFVPNGVLSNGTIINYTYATTRRADLTIGISYSSNIKRAKEIAMEVMEKHPM
                     VLKDPAPIVLVKDLAESSINLAVRPWSELPDFFEMRSDILEQIKNEFDANGITIPFPQ
                     RDIHIKQS"
     gene            complement(3383..3826)
                     /locus_tag="P5335_07680"
     CDS             complement(3383..3826)
                     /locus_tag="P5335_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRZ74794.1"
                     /translation="MGLGSFFKNLFGSAKESASEAVDKTEVVIDQAKEKATEYASKAE
                     EYVEKAVESAKESYPEVKEKVEQFAEKAKDTVTEYAEKVGDTVENVVDDVREKVHAFT
                     APAVEKTEETVSNFTEKADEKAEEVTEDIEEKVEEIKKAADENAD"
     gene            complement(3883..5898)
                     /locus_tag="P5335_07685"
     CDS             complement(3883..5898)
                     /locus_tag="P5335_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRZ74795.1"
                     /translation="MQNELTQETSPYLLQHANNPVHWKAWNSKTLTTAKEKKKLILVS
                     IGYSACHWCHVMEHESFENLEIAEVMNQHFINIKVDREERPDVDAIYMKAVQLMTGRG
                     GWPLNVVCLPDGKPVWGGTYFRKNDWINTLEQLQELFQNQPEKMLAYANKLHEGIESL
                     GLSKNEKGEINVTVLESFLSKWEKSFDWEYGGYAKAPKFMMPSNYQFLLQYSFLTQNE
                     KLVEYVNLTLTKMAFGGIFDTIDGGFSRYSVDVKWHVPHFEKMTYDNGQLLSLYSNAY
                     KLTKNELYKEIIEKTTTFIEKEWFTNLGGFYSALDADSNNQENQLEEGAFYVWTTEEL
                     KNLINDDYELFCLVFNINDFGHWEHGNYVLIQKESLAVISEEQNISLDQLKLKKKNWE
                     KRLYHYREKRAKPRLDDKCLTSWNAIMLKGFVESFKALENENYLKIAKKNAHFILEKL
                     WSSDGNLFHSYKNEKATINGFLEDYAFVIDAFIALYEATFEETWLEHAKNLTNYCLDH
                     FYDETKQLFRFTSDLDESLIVAHFETEDNVIPASNSVMAENLFKLSVYFENSYYEKVA
                     RLMLEQVLPSIDYPSAFSNWLSVLLRFSDYQKELAICGENISAYRLQLNRSYLPHVLL
                     SGCENNATLPFLKNRFIENKTLFYLCQNKTCLAPTTNFEEVYKDLKN"
     gene            complement(5957..6157)
                     /locus_tag="P5335_07690"
     CDS             complement(5957..6157)
                     /locus_tag="P5335_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010523308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HRZ74796.1"
                     /translation="MAILKVIEVLSSSEISWEEATRKAVAQASKSLKNIRSVYVQEQS
                     ALVDDGQITEFRVNVKLTFEIE"
     gene            6284..8539
                     /locus_tag="P5335_07695"
     CDS             6284..8539
                     /locus_tag="P5335_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRZ74797.1"
                     /translation="MDQIYIIMLFALALLAITDLMVGVSNDAVNFLNSAIGSKAVSFK
                     TIMIVASIGVAVGALYSNGMMEIARNGIFTPSMFSFNDVIIIFLAVMITDVLLLDVFN
                     TLGLPTSTTVSVIFSLLGASVVLAIYKVYVADGNMDNLGAYINTKKASEIVYSILLSV
                     ILSFGLGTLVQYISRLIFTFHIDKRYKYVGAVFGGVAMTAITFFILVKGLKSVTFVPS
                     EIKDFIKDNPFTIIGYSLVFWTIFCQFLMSIVKYNIFKFIIIVGTFGLALAFAGNDLV
                     NFIGVPIAAYQAYEIYIAPGQNLGATEFMMSGLEEENLPAPFLFLALAGAVMVYTLWT
                     SKKARTVIETEQNLSRQGDGTEKYNANTLSRNLVRAMLYVGNIISFILPKSLQEKLNA
                     QFIQPEVKGKRDKQPMFDMVRASVNLVVASVLISIGTSMKLPLSTTYVTFMVAMGSSF
                     ADRAWDRESAVYRVAGVFNVIGGWFVTAIVAFLSSAIIAYVLFIGEVYAFFAFMIVVA
                     IFFYRSNQIHKRKMKEEDDIKKLKKEDIVTIKEIINESSQQISRVIFTTNKVYTDVLD
                     NLGLQDLAKLKENKKALKKLEKEVDELKSNVYYFIKNLDETSVEASKFYVMILGYLQD
                     MIQSLAFITQNSYSHINNNHKQLKFNQIRDLKSIDVELQKLFDTIETIFKDQSFDRLD
                     EVLKEKNQILNNVSELIQKQITRIRTVETSPKNSKLYFGLLLETNDLIKATMNLLELF
                     KEFTIHVKSKK"
     gene            complement(8588..9487)
                     /locus_tag="P5335_07700"
     CDS             complement(8588..9487)
                     /locus_tag="P5335_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRZ74798.1"
                     /translation="MTKLSIKETQNPTILKFEFPDFITKSQSFEYKNIDDAKNSPLAQ
                     QLFYLPFVKTVFISGNFIAIERYNIVQWDDVKEAVAEQIETYVQNGGVILNFSESEIK
                     KQPITIYAESTPNPAVLKFVANKLLTTKTAEFKNIDETVASPLAKELFKFPYVKELFM
                     DENYISVTKYTSVEWQEISNEIRSFIKEYIENGHEIINESLLVTIPKNEKQQIANFDN
                     LDTTSQQIINILEEYVKPAVQADGGNILFDSYDEAEKRVKVVLQGACSGCPSSTFTLK
                     NGIENMLKDMLNDEAIKVEAINA"
BASE COUNT         3251 a         1489 c         1592 g         3365 t
ORIGIN      
        1 tccagttaac tattccaatt ttaaatggat ttgtaactaa aacgaatgtt gaaaaatcaa
       61 aagttgctgt tgaacgcggc aaaattgcct acgaacaaga agagttaaac ttgcaaagaa
      121 atgtgtatac agcgtttacg gatgcaaaag gagcattgaa ttcttatgaa gcttcactcg
      181 taactttaga agcaagacaa gaagctttta tttctgctaa agaacgattt gaggttggat
      241 taatgaatgc ttttgattat aatcaatcac aaacgttatt agtcaacgct caatcagaag
      301 tattacgaac caaatacgat tatattttta aaaccaaaat tttagaattt tattttggaa
      361 ttccaatcat tcaaaaacct taaattatgt caaagaaaaa agtatatatc tttttaggaa
      421 ttgctgttgt aatcattgta agtttaatta tcctatctaa acttggtatt ttagggaata
      481 aaaatattgg caaagaagta gaaattgcca cagttacaga aatgactatt attgaaacag
      541 tttctgcaac cggcaaaatt caaccggaaa cagagataaa aatatcttct gaagtttcag
      601 gtgaaattat tgatttacca attgttgaag gacaaatggt aaaaaaagga caattattag
      661 ttagaataaa ccctgattta tatacctcaa gtttaaatcg ttcggtagcc ggactttcaa
      721 acacaaaagc cggattaaat caagccgaag cttcattaaa agaagctcaa gcaaattaca
      781 accgaaataa atcattattt gaaaaaggaa tcatttctaa atcggagtgg gacaaaatag
      841 ttgctgttta tgaaggtgct caagcagcta aacaggccgc ctatttcggt gttcaaagtg
      901 catctgctac ggttacggaa gctagagata atttaggcag aacaacaata tattcacctt
      961 ccgatggaac aatttcgttg ttatcagtcg aattgggtga acgcgttttg ggaactcaac
     1021 aaatgaccgg aacagaaata ttaagagtag ccaacctcaa taatatggaa gtggaagtag
     1081 atgtgaatga aaatgatatt gtcaaaataa acattggcga ttctacaaac attgaagtag
     1141 atgcgtattt aaagaaagaa tttaaaggta ttgtgaccag tatttcaaat tctgccagta
     1201 ctgctgtaac ggcagatcag gttacaaact ttaaagtaaa agtaagaata ttaaaagaat
     1261 cttacttgga tttaattgaa ggaaaacctc taaattattc tccttttcga cctggtatga
     1321 ctgcaacggt tgatattatt accaaaagaa aagaaaatgt ggttgccgtt cccattagtg
     1381 ctgttgtgat gaaatcagat acgctgggta ctaataaagt agaagtaaaa caagatgata
     1441 aaaatgatga agaaggagaa gttgttaaaa aagtagataa aaaattagaa tgtgtctttg
     1501 ttaaagtggg ggataaagct aaaatcagaa tcattaaaac aggtattcaa gatgattcta
     1561 atattgaagt gatagaagga ttgaaaaaag gagatgtagt aattataggt ccttatacta
     1621 ccgtaacaaa agatattaat tcaggagata ctgtttttgt gaaaacggaa gaggaaaaag
     1681 caaaagaaaa ggaaaaattg aacaaaaaga aaaaataata ttttagaata aatttaaaag
     1741 gaagctttat ttagtttcct ttttttatgt cttatttttg catcgataaa accaaagtat
     1801 ggtctatata ctcaatattg aaacttctac taaaaattgc tcagtagccg tagcctgtga
     1861 gggaaaagtt attcttagta aagaaatagc tgaaattggc tattctcatg ctgaaaaatt
     1921 gcacgtgttt ttggaagaat tgcttaagga aacagatctt agttttaagg atataaatgc
     1981 catagcagtt agccaaggtc ccggttctta taccggttta cgtattggtg tttcagcatc
     2041 aaaaggactt tgttatgcac tagatattcc tttaattgct gttgatacga tgcaaatttt
     2101 ggcaaatcag gtaacagttg aggaaggtat tatcatccct atgattgatg ccagacggat
     2161 ggaagttttt tcagcgattt ttgacaaaaa ccaccttaaa attcgtgaaa caaaagcaga
     2221 aattttagaa gaagattctt tttcaattaa taatggacca atttatatta ttggtgattg
     2281 tgcttccaaa gtcaaaactg tcttaacgca tgagaaattt gtttttctag aaaaccatat
     2341 ttttccctct gcaaaagaaa tggccacctt aagttataga ttatttaaac aaaataactt
     2401 cgtggatgtt gcctattttg aaccttttta tttaaaggat tttatgctaa ctcaaaaaag
     2461 ttaagactgt ttaatgtgaa tatccctttg cggaaaagga attgtgatac cattcgcatc
     2521 aaattcattt ttaatttgtt ctaaaatatc agaacgcatt tcaaaaaaat caggcaattc
     2581 agaccaaggt cttacagcta aattaattga actttctgct aaatctttaa ccaatacaat
     2641 aggagcaggg tcttttaaaa ccattggatg tttttccatc acttccatgg caatttcttt
     2701 tgctcgtttt atattggaac tgtaactgat tccaattgtt aaatcggctc ttctagtggt
     2761 agcatacgtg taattaatga ttgttccgtt agacaaaacg ccattgggaa caaaaatgat
     2821 ttgattatca acagtcgata attgcgtcac gaaaatctga atttgtttta cggttcccat
     2881 aacgccttgt gcttctataa aatcgccaac cttaaaaggt ttgaacatga taattaaaac
     2941 acctccggca aaatttgata gcgaaccttg caaggaaagt ccaacagcta aaccggcagc
     3001 accaataatg gcgacaaagg aagaattttc aacacctaag cgggatatta cagttacaaa
     3061 gagtaaaatt ttaagagtcc aaatcaaaat atccgctaga aattttgaaa gagttggttc
     3121 catttctctt ttgaccataa tacgcatgat taatttttta atcaatttaa tcacagtcca
     3181 ccctaccaca aaaataatag ccgcagaaat taatcgaggg gaatattcaa ttaatacagt
     3241 tgtaaaataa tcccagtaac gggtaataaa atcagtgttt agcgttatca attccataaa
     3301 aaaactctca aacaattaat tcgtttgaga gttgcaagat aagattttaa ggtctgtttt
     3361 tgttttaaaa aaaacctaaa atttagtcag cattttcatc tgctgctttt tttatttctt
     3421 ctactttttc ttctatatct tcagtcactt cttccgcttt ttcatctgct ttttcagtga
     3481 aatttgatac ggtttcttcg gttttttcaa cagctggagc agtaaatgca tgcacttttt
     3541 ctctcacatc atctactaca ttttcaacgg tatcaccaac tttttcagca tattctgtaa
     3601 ccgtgtcttt tgctttttct gcaaattgtt caaccttttc ctttacttca ggataagatt
     3661 cttttgcact ttctaccgct ttttctacat attcttccgc ttttgaggcg tattctgttg
     3721 ctttttcttt ggcttgatcg attacaactt cagttttgtc aactgcttct gatgccgatt
     3781 ctttggctga accaaataaa tttttaaaaa aacttcctaa tcccatggtt ataatttttt
     3841 agtgaatgcc aaatctacta aattttgcta acttttttaa aattagttct ttaaatcttt
     3901 gtaaacttct tcaaagttgg tagtgggagc caaacatgtt ttgttttgac aaagataaaa
     3961 aagggttttg ttttcaataa atcgattttt taaaaaaggt aaagtagcgt tgttttcaca
     4021 tccggaaagg agaacatgtg gtaaataact tcggtttaat tgcaatcggt atgctgatat
     4081 attttcccca caaatcgcca attctttttg gtaatctgaa aaacgcaata atacggatag
     4141 ccaatttgaa aaagcggaag ggtaatcaat tgagggcaaa acttgttcaa gcattaaacg
     4201 ggcgactttt tcataatatg aattttcaaa atacacactc aatttgaata aattttcagc
     4261 cattacggag ttagaggccg gaatcacatt atcttccgtt tcaaaatgag caacaatcaa
     4321 agactcgtct aaatcagaag taaatcggaa aagttgtttg gtttcgtcat aaaaatgatc
     4381 taaacaatag ttggttaagt ttttagcatg ttcaagccaa gtttcctcaa atgtagcttc
     4441 atataaggca ataaaagcat caattacaaa agcataatct tccaaaaaac cattgattgt
     4501 agctttttca tttttatagg aatgaaacaa atttccatca ctagaccaaa gtttttctag
     4561 aataaaatga gcattttttt tagcaatttt taaatagttt tcattttcta atgccttgaa
     4621 actttctaca aatcctttca acatgattgc attccaagaa gtaaggcatt tgtcatctaa
     4681 tcgtggtttt gctctttttt ctcgatagtg atagagtctt ttttcccagt ttttcttttt
     4741 tagtttgagt tggtcaagag agatattttg ctcttcagaa attacggcta aactttcttt
     4801 ttgaatcaaa acgtagtttc catgttccca atgtccaaaa tcattgatgt tgaaaaccaa
     4861 acagaataat tcataatcat catttatcag gttttttaat tcttctgttg tccaaacata
     4921 aaaagctccc tcttctaact gattttcctg gttgttacta tcagcatcta atgcagaata
     4981 aaaacctccc aaattagtaa accactcttt ttcaataaat gttgtcgttt tttcaataat
     5041 ctctttatat aattcgtttt tagtaagttt gtaagcattc gaatacagtg acaaaagttg
     5101 cccattgtca taggtcattt tttcaaaatg aggaacatgc catttgacat caacggaata
     5161 gcgtgaaaaa ccaccatcaa tcgtatcgaa aattcctcca aaggccattt ttgtcaaggt
     5221 taaattcaca tattccacta atttttcgtt ttgagtcaaa aagctatact gcaaaagaaa
     5281 ttgataatta ctgggcatca taaatttagg agccttagca taacctccat attcccaatc
     5341 aaaacttttt tcccatttag acaaaaagga ttctaaaacg gtgacattaa tttctccctt
     5401 ttcatttttt gataaaccaa gtgattcaat tccttcatgt aatttattgg catatgccaa
     5461 cattttttcg ggttggtttt gaaaaagttc ctgtaattgt tccaaggtat ttatccagtc
     5521 attttttctg aaataagtgc ctccccaaac aggttttcca tccggaaggc aaacaacatt
     5581 gagcggccat cctccacgac cggtcattaa ttgtaccgct ttcatataaa tagcatctac
     5641 atctggacgt tcttcacgat caactttaat gttgataaaa tgttgattca tcacttcggc
     5701 aatttctaga ttttcaaaac tttcatgttc catcacatga caccaatgac aggcggaata
     5761 accaatgctt actaaaataa gtttcttttt ttctttcgct gtagtcaacg ttttagaatt
     5821 ccaagctttc caatgaaccg gattgttggc gtgttgtaat aaatagggac tggtttcttg
     5881 cgtgagttcg ttttgcatag ttgaaaaaat aaaaagcgtt ccttttgaga acgcttaaaa
     5941 atacaatttt aatacattac tcaatttcaa aagtgagttt tacatttaca cgaaattctg
     6001 tgatttgtcc gtcatcaact agagcacttt gctcctgaac ataaacggag cgaatatttt
     6061 ttaacgattt tgatgcttga gcaactgctt tccgtgttgc ttcttcccaa ctgatttcag
     6121 aactggaaag aacttcaatt acttttaaga tagccatgtt ttataattta gtatttaaag
     6181 ataccttttt ttattaattt ttcaaaaaaa ttgtctaaaa ttgataacat tagcttaaca
     6241 tttgattgaa atagttaacg gaattttgca aaaaattatc ttgatggatc agatatatat
     6301 tatcatgctt tttgcacttg ccttattggc aattactgat ttaatggtgg gtgttagtaa
     6361 tgatgctgtt aactttttaa actctgcaat cggttctaag gctgtttcgt ttaaaaccat
     6421 aatgattgtt gcaagtatcg gcgtggcagt tggagcatta tattcaaacg gaatgatgga
     6481 aattgccaga aatggaattt ttacacccag tatgtttagc tttaatgatg taattatcat
     6541 ctttttggca gtaatgatta cggatgtttt actgttagac gtgtttaata ctcttggttt
     6601 gcctacttct acaactgttt ccgtaatatt ttcattatta ggagcttctg ttgttttagc
     6661 aatttataaa gtttatgttg ctgatggcaa tatggataat ttgggggctt atataaatac
     6721 taaaaaagca tcagagattg tttacagtat cttgctgtca gttatacttt cttttggtct
     6781 tggaacttta gtgcagtata tttcaaggct gatttttaca tttcatattg ataagagata
     6841 taaatatgtt ggagcagttt ttggtggtgt tgctatgact gctattactt tttttattct
     6901 tgtaaaagga ttaaaatcag ttacttttgt gccttcagaa ataaaagatt ttatcaaaga
     6961 taatccattt acgattatcg ggtattcgct tgttttttgg acgatttttt gtcaattttt
     7021 aatgagtatt gtcaagtaca acattttcaa atttattatt atagtcggta cttttggttt
     7081 ggctttggct ttcgccggaa atgatttagt taattttata ggtgtaccta ttgctgctta
     7141 ccaagcatat gaaatttata ttgctccggg tcaaaatctg ggggcgacag agtttatgat
     7201 gtccggctta gaggaagaaa atttacctgc tccgtttttg tttttagcat tagcaggggc
     7261 tgttatggtt tatacattat ggacatcaaa gaaagcaagg actgtaattg aaacagaaca
     7321 aaacctttct cgtcaaggtg atggaacaga aaaatataat gctaatacac tttccagaaa
     7381 cttagttaga gcaatgttat atgttggaaa tataataagt ttcatactcc caaaatcatt
     7441 acaggaaaaa ttaaatgctc agtttattca acctgaagta aaagggaaaa gagacaaaca
     7501 acccatgttc gatatggttc gagcttcagt aaatttagtt gtggcctctg tcttaatttc
     7561 aattggaacc tctatgaaat taccactttc tacaacttat gtaactttta tggtggctat
     7621 gggatcctct tttgcagata gagcttggga cagagaaagt gcagtttaca gagttgcagg
     7681 agttttcaac gtcataggag gttggtttgt aacagctatc gtggcttttt tatcttctgc
     7741 cattattgct tatgttttat ttattggaga agtttatgct tttttcgctt ttatgattgt
     7801 agtagctatt ttcttttaca gaagtaatca aattcacaaa agaaaaatga aagaggaaga
     7861 tgatattaaa aaattaaaaa aggaagatat tgttactatt aaagaaatta taaacgaaag
     7921 ttctcaacaa atatctagag taatatttac aacaaataaa gtttatacag atgttctcga
     7981 taatttaggt ctacaagatt tggctaaact taaggaaaac aaaaaagcat taaaaaaact
     8041 tgaaaaagag gttgatgaat tgaaaagtaa tgtttactac ttcatcaaaa atttagatga
     8101 aacatccgta gaagccagta agttttatgt aatgattttg ggttatctgc aagatatgat
     8161 acagtcgttg gcttttataa ctcaaaatag ttattcacat attaacaata atcacaaaca
     8221 actgaaattc aatcaaatta gagatttgaa aagtattgat gttgaattgc aaaaattatt
     8281 tgatactata gaaacaattt ttaaagacca aagttttgat agattagatg aggttttaaa
     8341 agagaaaaat caaattctaa ataatgtctc ggaattaatt caaaaacaaa tcacacgaat
     8401 cagaacggta gaaaccagtc caaaaaacag taaattatat tttggattgt tattagaaac
     8461 aaacgatttg ataaaagcaa ccatgaattt actggaactt ttcaaagagt ttaccattca
     8521 tgtgaaaagt aaaaaataaa gcaaaaatcc ttgagtaatc aaggattttt gcttttaaaa
     8581 aagtttgtta tgcgttaatt gcttctactt ttatggcttc atcattcagc atgtctttta
     8641 acatgttttc aataccgttt ttcaaggtaa atgtggaaga aggacagccg ctacaagccc
     8701 cttgtaaaac aacttttact cttttttcag cttcatcata ggaatcaaac aatatatttc
     8761 caccatcggc ttgaacagcc ggttttacgt attcctctaa aatattgata atttgttggg
     8821 atgtagtatc taagttatca aaattagcaa tttgctgttt ttcatttttt ggaatagtaa
     8881 ccaataaact ttcattgata atttcatgac cgttttcaat atattcttta atgaatgaac
     8941 gaatttcatt tgaaatttcc tgccattcca cactggtata ttttgtgacg gaaatgtagt
     9001 tttcatccat aaaaagttct ttcacatacg gaaatttgaa tagttctttg gctaagggag
     9061 aggcaactgt ttcatcaata tttttgaatt cggcagtttt agtagttaaa agtttattgg
     9121 caacaaattt aagaacagcc ggatttggtg tactttcggc ataaatcgta attggttgtt
     9181 tttttatttc gctttctgaa aaatttagga tgacaccacc attttgaaca taagtttcaa
     9241 tttgttccgc cacagcctct ttaacatcat cccactgaac gatattgtat cgttcaatgg
     9301 ctataaaatt tccggatata aaaacagttt ttacaaaagg taaataaaat aattgttgtg
     9361 ccaacggact gtttttagca tcgtcaatgt ttttatattc aaaactttga ctttttgtaa
     9421 taaaatccgg aaattcaaat tttagaatgg ttggattttg tgtttctttt atcgataatt
     9481 ttgtcatttg accttttttg tgcaaattta acaaacttat ttttggcaaa tagttatatt
     9541 tgaagattaa atttatatta acaaaacttt atcaagtgtt ttattgttta ttgaaaacat
     9601 gaaagacttg ctttttttat atgaaaaaac tactactttt cgctgtattt cttatacttc
     9661 cctactgttc caatgctcaa ttatttgtta ataatgt
//
LOCUS       DAOTCW010000083         9687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_4717_length_9687_cov_46.260797, whole genome shotgun sequence.
ACCESSION   DAOTCW010000083 DAOTCW010000000
VERSION     DAOTCW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4717_length_9687_cov_46.260797"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="P5335_07705"
     CDS             <1..604
                     /locus_tag="P5335_07705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRZ74799.1"
                     /translation="DFGAGSRVFKSNQRKISAIAKNAGISPKRAELLFRIVHYFQPEN
                     SLEIGTSLGLATIALSLGNKNSKVITLEGCPQTMAIAKNQCKLQNLNNIEFVNTKFED
                     YLKNLQPAPETSGHNPQLIYFDGNHSKNATLEYFQLLLPTITNDSVWIFDDIHWSKDM
                     EEAWKIIKNHPKVTVTIDTFQWGMVFFRCEQEKEHFTIRT"
     gene            complement(657..1313)
                     /locus_tag="P5335_07710"
     CDS             complement(657..1313)
                     /locus_tag="P5335_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74800.1"
                     /translation="MKKSKLIFGISILVIGFTSCKDEKNDQAEKTVETYVVYVDSVET
                     ISPEEAKTNWAVIETNYQARNAAAQEALADLKDKEVAQAKIDASNAKYEALKAKLEAA
                     NQAAVSPKQQLRNALFGEGKIGDDMSFSWVNKDNLLTTFKTFTETVQNNKDAYTREDW
                     DEIKLLYEALGTRKNTVEKEGLSSSDNNEIAGIKIKFAPMLKVNRMGAKAEENAAAKE
                     "
     gene            1644..2333
                     /locus_tag="P5335_07715"
     CDS             1644..2333
                     /locus_tag="P5335_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRZ74801.1"
                     /translation="MAQPLIKITNIKRDFILGSETIYVLKGIDLEINKGEYVALMGPS
                     GSGKSTLMNLLGCLDTPTSGTYILNGKDVSQMHDDELAEIRNKEIGFVFQTFNLMPRT
                     SALDNVALPMVYAGYPKSERNIRATEVLTQVNLADRMDHKPNELSGGQRQRVAIARAL
                     VNNPSIILADEPTGNLDSKTSEEIMGLFDVIHKNGNTIILVTHEEDIAAHAHRIIRLR
                     DGMVEKDEQVK"
     gene            2443..>3328
                     /locus_tag="P5335_07720"
     CDS             2443..>3328
                     /locus_tag="P5335_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74802.1"
                     /translation="MEIDEIVISRLVIIPKRNINRMIVGILTCARLPELLESDQKLIP
                     LFAKENITAKAVIWDDESINWTEFDYLIFRNTWDYYQKEIAFNHWLDKIESLGIKTLN
                     PITTIQKNKHKFYLKKLEKEGISILPTLFLEKNSTPDLLNLIPENWKKIVIKPAFSAG
                     SYLTKLIDRSEIDSIQEEFKDHFKTKDFLLQEFRPEIKELGETSLIFFNGNFSHAINK
                     KPVSTDFRVQIQYGGKYTLIEPNDDLILQAELVLSKIPEKILYARVDGIVIKNKLHLM
                     EVELIEPDLYFDLADGARE"
     assembly_gap    3329..3428
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3631..4203
                     /locus_tag="P5335_07725"
     CDS             3631..4203
                     /locus_tag="P5335_07725"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008272288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRZ74803.1"
                     /translation="MKIYTKTGDSGTTALFGGTRVPKHHIRIESYGTVDELNSHVGLI
                     RDQNINQLYKNILIEVQDRLFTVGAILATPPEKEILKNGQPRLNINRISEEDIQLLEN
                     EIDSMNDSLPPMTHFVLPGGHTTVSYCHIARCVCRRAERLSVHLHELEPTDEQVLKYL
                     NRLSDYLFVLARKLSVDLNAEEVKWIPRKE"
     gene            4366..4587
                     /locus_tag="P5335_07730"
     CDS             4366..4587
                     /locus_tag="P5335_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRZ74804.1"
                     /translation="MYWTLELASYLSDAPWPATKDELIDYAIRTGAPLEVVENLQSIE
                     DEGEIYESMEEIWPDYPTDEDYLWNEDEY"
     gene            4672..8022
                     /gene="secA"
                     /locus_tag="P5335_07735"
     CDS             4672..8022
                     /gene="secA"
                     /locus_tag="P5335_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRZ74805.1"
                     /translation="MSFINSILKAFVGDKSEKDVKAIQPLLNKIKTYESNLASLSHDE
                     LRAKTTAFKETIKLARAEKDNKISSLKTEVELILDIDKREDLYLAIDALEKEAYEISE
                     KVLLEILPEAFAVVKETAKRFKDNTSIKVKATPKDIELSATKSYITLEGNDAIWANSW
                     NAAGKEITWDMIHYDVQLIGGVVLHQGKIAEMQTGEGKTLVATLPLYLNALTGNGVHL
                     VTVNDYLAKRDSTWKAPLFEFHGLTVDCIDNHQPNTDARRKAYEADITYGTNNEFGFD
                     YLRDNMAHSPNDLVQRKHNYAIVDEVDSVLIDDARTPLIISGPVPQGDRHEFNELKPK
                     VDNLVSLQRNLATGFLTEAKRLIKEGNAKDGGFNLLRAYRALPKNKALIKFLSEEGVK
                     QLLQKTENQYMQDNNREMPKVDEALYFVIEEKNNQVELTDNGIKFLSQDTDGNFFVLP
                     DIGTEIARIEKLHLSKDQEAEEREKLFQDFSIKSERIHTLTQLLKAYTLFEKDVEYVI
                     MEDKIMIVDEQTGRIMDGRRYSDGLHQAIEAKENVKIEAATQTFATITLQNYFRMYSK
                     LAGMTGTAVTEAGELWQIYKLDVVEIPTNRPMSRKDQEDLIYKTTREKFNAVIEDVTK
                     LSQAGRPVLIGTTSVEISELLSRMLKMRNIPHNVLNAKMHKKEAEIVAEAGKPGVVTI
                     ATNMAGRGTDIKLTPEVKAAGGLAIIGTERHDSRRVDRQLRGRAGRQGDPGSSQFYVS
                     LEDNLMRLFGSERVAKVMDRMGLKEGEVIQHSMMTKSIERAQKKVEENNFGVRKRLLE
                     YDDVMNSQREVIYKRRRHALHGERLKVDIANMMYDTCENIVNENKAISNFKNYEFELI
                     RYFSITSPISESEFNKLTEIEITGKIYKAALAHYEEKNERDAREAFPIIRNVYENPSN
                     HYERIVVPFSDGQKTLNVVTDLKKAYDSEGKQLVADFEKNITLAIVDEAWKKHLRKMD
                     ELKQSVQLAVHEQKDPLLIYKFEAFNLFKAMLDKVNKEVISFLFKGDLPNRNETNIQE
                     AKEIRQKEDYKTSKDEIPNSDSLAAQHREAGQTQRQPITETIVRDTPKINRNDTVTIK
                     HVMSGKTESMKFKKAESMIASGDWVLVN"
     gene            8101..8736
                     /locus_tag="P5335_07740"
     CDS             8101..8736
                     /locus_tag="P5335_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74806.1"
                     /translation="MKKTVILFFIFVSIQTLYSQEKNNSPFLEPKNQFKVEVDSKEYF
                     YSEGDELEINHSATKSKLKIELLGFKKFDSGTVSFHYPSNFAFEVDKDFGYNNWTFDG
                     NNVVILYFEIDNDMKMQEYIDLMVEQFGKKNCKTEKKQIQLGNKTLSGTRINITLVGQ
                     LLSLEFFEIPLNDYKTRFIAFQDSKDEEGNNSKELADTIKMVHETITFLEN"
     gene            8742..9314
                     /locus_tag="P5335_07745"
     CDS             8742..9314
                     /locus_tag="P5335_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74807.1"
                     /translation="MKKIINLLLILNFTVFYSQSIENLKIETKNFYAAHYNMDFEGIA
                     SLYHPKYFEKFTKETITAGLDERFQNDVDGIRFVFPTMTFNYAPIQEINSGKYCVITF
                     KNSFRIIKSNPQAESQVQKDLASYLKIKNYKSVKFESKRNSYFIEENVTWIAVSSAQT
                     SWKWTFIEMNSTFKSLDEFLDTSLQKELGL"
     gene            complement(9506..>9687)
                     /locus_tag="P5335_07750"
     CDS             complement(9506..>9687)
                     /locus_tag="P5335_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRZ74808.1"
                     /translation="EIFELTVSLKGTLSGEHGIGYVQKDYMPIAFSKTHLELMERIKF
                     VFDPNGILNPGKILP"
BASE COUNT         3509 a         1541 c         1634 g         2903 t
ORIGIN      
        1 cgattttggt gccggttcaa gagtttttaa atccaatcaa agaaaaattt ctgctattgc
       61 aaaaaatgcc ggaatttctc caaaaagagc agaattatta tttagaattg ttcattattt
      121 tcaacctgaa aatagtttag aaattggaac ttcgttggga ttagccacca ttgctctttc
      181 tttaggaaat aaaaattcaa aagtaattac tttagaaggt tgccctcaaa caatggcaat
      241 agctaaaaat caatgtaaat tgcaaaattt aaacaacatt gaatttgtaa acactaaatt
      301 tgaagactac ctcaaaaacc ttcaacccgc tcccgaaact tcgggacaca acccgcaact
      361 aatttacttt gacggaaatc attcgaaaaa tgctacatta gaatatttcc aattattatt
      421 acccacaatt acaaacgatt ctgtgtggat ttttgacgat attcattggt caaaagacat
      481 ggaagaagct tggaaaataa ttaaaaatca ccccaaagtt accgtgacta ttgacacctt
      541 ccaatgggga atggttttct tcaggtgcga acaagaaaaa gaacatttca ccattcgaac
      601 ataaaaaaaa tgacagccat cactgactgt cattttccaa ctgaactaat aactaattat
      661 tctttcgctg cagcattttc ttctgcttta gctcccatac ggtttacttt caacattggt
      721 gcaaatttga tttttatacc tgcaatttca ttgttgtcag aagatgataa accttctttt
      781 tcaacggtat ttttacgtgt tcctaaagct tcatacaaca atttgatttc atcccaatct
      841 tcacgggtat aagcatcttt attgttttga actgtttcag taaacgtttt aaaagttgtc
      901 agaaggttat ctttgtttac ccatgagaaa ctcatgtcat caccaatttt tccttcacca
      961 aacaaagcat tacgcaattg ttgttttgga gaaactgctg cttggtttgc agcttctaat
     1021 tttgctttta atgcttcata ttttgcatta ctagcatcaa ttttagcttg ggctacttct
     1081 ttatctttta aatctgccaa agcttcttga gctgcagcat ttcttgcttg ataattagtt
     1141 tcaataactg cccaattggt tttggcttct tccggactta tggtttcgac tgaatcgaca
     1201 taaaccacat aagtttcaac tgttttttca gcttgatcat ttttttcatc cttacaagag
     1261 gtaaacccta ttacaagtat tgaaattcca aaaattaatt tacttttttt cattttataa
     1321 gtttttaaat aatattctgt aaatgtaatg taaattatat aataatcatt attttaaatg
     1381 ttttattaaa tttattttaa aaatttgtta tattttttac aaaatatgtg aattatgtaa
     1441 tatttttaca aaattatata actttaactt tgcataaaaa acatacatta tgatttctaa
     1501 actacaaaga aagattgaag ttaaaaattc aataatgtaa caatttgata aatttgagta
     1561 cttatagcat aaacaaaaat caaatttgta cttttaaccg aagagtttct aaatattatt
     1621 ttaaaactac aatcttttac taaatggctc aaccacttat caaaataacc aatattaaac
     1681 gagattttat tctaggaagt gaaacaatat acgtacttaa aggaatcgat ttagaaatca
     1741 acaaaggaga atatgtagca ttgatgggac cttcaggatc cggaaagtca actttgatga
     1801 atcttttggg atgtttagac actcctacct cgggaacata cattctaaac ggaaaagatg
     1861 tgagtcagat gcacgatgat gaattagccg aaatacgtaa taaagaaata ggttttgtat
     1921 ttcaaacatt caacttaatg cctcgaacat cagctttgga taatgtggct ttaccaatgg
     1981 tttacgcagg atatccaaaa agcgagcgaa acataagagc tactgaagtt ttaacacaag
     2041 taaatttggc cgaccgtatg gaccacaagc caaatgaact ttcgggcggt caacgtcagc
     2101 gggttgctat tgcacgagcg ttggttaata acccttccat tattttggct gatgagccta
     2161 ccggaaactt ggacagtaaa acctctgaag aaatcatggg tctttttgat gtcattcaca
     2221 aaaatggaaa tacaattatt ttggtaactc acgaagaaga tattgctgct catgctcatc
     2281 gaatcattcg tttacgtgac ggaatggtgg aaaaagatga acaagtaaaa taaacaattt
     2341 gtacctttgt gtattaagtt tattttatga aaacacttat tattattctt gtcgttttat
     2401 ttatagccgt tgctattatt actcgaatgg caaacaaaca ccgtggaaat cgacgaaatc
     2461 gtaatttcaa gactagttat tattccaaaa agaaacatta accgaatgat tgttggaata
     2521 ctcacttgtg cccgattacc tgaactttta gaatcggatc aaaaattaat tccgcttttt
     2581 gcaaaagaaa atattactgc aaaagcagtt atttgggatg atgaatcgat taattggact
     2641 gaatttgatt accttatttt cagaaataca tgggattatt atcaaaaaga aattgcgttt
     2701 aaccattggt tggataaaat tgaatcgtta ggaatcaaaa cgttgaatcc aattacaaca
     2761 atacaaaaaa acaaacataa attttactta aaaaaattag aaaaagaagg aatttcaata
     2821 cttccaactc tttttttaga aaaaaattca acgccggatt tacttaattt aattcctgaa
     2881 aactggaaaa aaattgtcat caaacctgct ttttctgccg gaagttattt aactaaatta
     2941 attgaccgaa gtgaaattga ttccattcaa gaagaattca aagaccattt taaaaccaaa
     3001 gatttccttt tacaggagtt tcgtcctgag ataaaagaat tgggcgaaac atcgctgatt
     3061 ttctttaacg gaaatttttc acatgcaatc aacaaaaaac cggtttcgac cgatttcaga
     3121 gttcaaattc aatacggtgg aaaatatact ttaattgaac ccaatgatga tttaatactt
     3181 caagccgaat tagttctttc aaaaattccc gaaaaaattt tatatgctcg cgtagacggt
     3241 atcgttatca aaaataaact tcatttgatg gaagttgaac tcattgagcc cgatttgtat
     3301 tttgatttgg ctgacggggc aagagaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnngt tgaactcatt gagcccgatt tgtattttga tttggctgac ggggcaagag
     3481 aaagatttgt ggaagctttt ttggaggttt tgaggatcta tggttcttag tctctaaggt
     3541 taatctaaaa ccacttaaaa ctacaaaatc attcaactca ataaaaaaac ttagtacctt
     3601 agcaactcag aacctcagca acttaaaaaa atgaaaatat ataccaaaac cggcgatagc
     3661 ggtaccaccg cactttttgg cggaactaga gttcccaaac atcacattcg tattgaaagt
     3721 tatggaacag tagacgaatt aaattcgcac gtcggactta ttcgcgatca aaatatcaat
     3781 caattatata aaaatatcct aattgaagta caagaccgtc tttttaccgt tggagccatt
     3841 ttggccacac ctcctgaaaa agaaatttta aaaaatggtc aaccgcgttt aaacatcaat
     3901 cggatttcgg aagaagacat tcaattattg gaaaatgaaa tcgattcgat gaacgattca
     3961 ttacctccaa tgacgcattt tgttttaccc ggcggacaca caacagtgtc atattgtcat
     4021 atcgcaagat gtgtttgccg aagagccgaa cgtctatccg tgcaccttca cgaattagaa
     4081 ccgacagatg agcaggtttt aaaatattta aaccgccttt ctgactacct ttttgtcttg
     4141 gcacgaaagt tgtcagttga cctgaacgcg gaagaagtaa aatggattcc gaggaaagag
     4201 taagctatgt tcagttgccc gctagcaatg ttcaagaaag aagctcaaaa aacacgttct
     4261 taacttttaa aaagtaaaaa aaatactttt tggtaaaata aatcactttt tacttgacta
     4321 tttcagcaga aaaattattt ttgcataaaa ttaaatcgat agaagatgta ttggacatta
     4381 gaattagcat cctatttaag tgatgcaccg tggccagcca ccaaagatga gcttattgat
     4441 tatgctatca gaacgggtgc tccgcttgaa gtagtggaaa accttcaatc tatagaagat
     4501 gaaggcgaaa tctatgaatc gatggaagaa atttggccgg attatcctac agacgaagat
     4561 tatctttgga acgaggacga atattaaaga aaaataaata aatcaccaga aagaagtctc
     4621 actagaggct ttttttttgt gtaaatttgc acacttaaaa aaacaataat tatgagtttc
     4681 ataaatagta ttcttaaagc atttgttggc gacaaatccg agaaagatgt caaagcaata
     4741 caaccccttt taaataaaat taaaacatat gaaagcaatt tagcatcgct ttcacatgac
     4801 gaactgcgag caaaaactac agctttcaaa gaaactataa aactagcccg tgctgaaaaa
     4861 gacaataaaa tttcttcctt aaaaacagaa gtggagctga ttttagatat cgataaacgc
     4921 gaagatttgt atttggcaat cgatgcgttg gaaaaagaag cttatgaaat ttctgaaaaa
     4981 gttttattgg aaattctacc ggaagctttt gctgtagtta aagaaacggc taaacgtttc
     5041 aaagataata cttcaattaa agtaaaagca acgccaaaag acatcgaact ttcagcaaca
     5101 aaatcatata tcacacttga aggtaacgat gcaatttggg ctaattcatg gaatgctgcc
     5161 gggaaagaaa ttacttggga catgattcac tatgatgttc agttgattgg tggtgtcgtt
     5221 ttacatcaag gaaaaattgc cgaaatgcaa actggggaag gaaaaacatt agttgctacg
     5281 ctaccccttt atttgaatgc cttaacagga aacggtgtgc atctagttac cgtgaatgac
     5341 tatttagcaa aacgggatag cacatggaag gctcctttat ttgaatttca tggtttaact
     5401 gttgattgta ttgacaatca tcaaccaaat accgatgcca gaagaaaagc atacgaagca
     5461 gacattacct acggaacaaa taatgaattt ggttttgact atttaagaga taatatggca
     5521 cattcaccaa acgatttggt gcaacgcaaa cacaattatg ccattgtcga tgaggttgac
     5581 tccgtattga ttgatgatgc cagaacacct ttaattattt caggtccggt tccgcaagga
     5641 gacagacatg aatttaatga attgaagcca aaagttgata acttagtttc tttacaacga
     5701 aatttagcta caggattttt aacagaagca aaacgtctaa ttaaagaagg taatgctaaa
     5761 gatggcggtt ttaacttgtt aagagcttat cgtgccttac ctaaaaacaa ggcgttaatt
     5821 aaattcttaa gtgaagaagg tgttaaacaa ttacttcaaa aaactgaaaa tcaatacatg
     5881 caagataata atcgcgaaat gcccaaagtg gatgaagcat tatattttgt tattgaagaa
     5941 aaaaataatc aagttgaact aaccgataac ggaatcaaat ttttatcgca agacacagat
     6001 ggaaacttct ttgttttacc ggatatcgga actgaaattg cccgtattga aaaattgcat
     6061 ttaagtaagg atcaagaagc tgaagaaaga gaaaaattat ttcaggactt ttctatcaaa
     6121 tctgaacgta tccacacatt aactcaatta ttgaaagcct ataccctctt tgaaaaagat
     6181 gtggaatacg taatcatgga agacaaaatt atgattgtcg atgaacaaac cggtcgtatt
     6241 atggatggtc gtcgttattc tgatgggtta caccaagcga ttgaagccaa agaaaacgta
     6301 aagattgaag cagccactca aacttttgca acgattacgc ttcaaaatta tttccgtatg
     6361 tacagcaaat tagccggtat gacgggaact gcagtaacag aagcaggaga gctttggcaa
     6421 atttacaaat tggatgtagt ggaaattcca actaaccgtc cgatgtcaag aaaagatcag
     6481 gaagatttaa tatacaaaac aactcgcgaa aaattcaatg ctgttattga agatgtaacc
     6541 aaattgtctc aagcaggaag gccggttttg attggaacta catcagtaga aatttcagaa
     6601 ttattgagtc gaatgttgaa aatgcgtaac attccgcaca atgtgttgaa tgcaaaaatg
     6661 cataagaaag aagctgaaat tgttgccgaa gcgggtaaac ctggagtcgt aacaattgca
     6721 acaaatatgg ccggtcgtgg taccgatatt aaactgacac cggaagttaa agccgcagga
     6781 ggtttagcca tcattggaac tgaacgtcac gattctcgtc gtgtagaccg tcagttacgt
     6841 ggtcgtgccg gtcgtcaggg agatccggga agttcacagt tttatgtgtc tcttgaagat
     6901 aatttgatgc gtttatttgg ttcagaacgt gtagccaaag taatggacag aatgggtttg
     6961 aaagaaggtg aagtgattca acattccatg atgaccaaat ccatagaaag agctcagaaa
     7021 aaagtagaag aaaacaactt tggcgtgcgt aaacgtttat tggaatatga tgacgtaatg
     7081 aattcacaaa gagaagtaat ctacaaacga agaagacatg cgttacacgg agagcgattg
     7141 aaagtggata tcgcgaacat gatgtatgat acgtgtgaaa atatcgtaaa cgaaaacaaa
     7201 gcaatttcta attttaaaaa ttatgaattt gagttaattc gctatttttc aattacatca
     7261 cccatttcag aaagcgaatt taacaaactt actgaaatag aaattaccgg aaaaatttat
     7321 aaagcagcgt tagcacatta tgaggaaaaa aatgaacgag atgctcgtga agcttttcct
     7381 atcatcagaa atgtttatga aaatccatct aatcattacg aacgaattgt agttccattt
     7441 agtgatggtc aaaaaacatt aaatgtggtt accgatttga aaaaagctta tgattcggaa
     7501 ggaaaacaat tggtcgctga ttttgaaaaa aatatcacct tagccattgt tgatgaagct
     7561 tggaaaaagc atttacgcaa aatggatgaa ttgaaacaat ctgttcaatt agctgttcac
     7621 gaacaaaaag atccattgtt gatttataaa tttgaagctt tcaatttatt taaagcaatg
     7681 ctggataaag tgaataaaga agtgatttcc tttttattca aaggtgattt accaaataga
     7741 aatgaaacaa atattcagga agccaaagaa attcgccaaa aagaagatta taaaacatca
     7801 aaagatgaaa tcccaaacag cgattcttta gccgcacaac atcgtgaagc cggtcaaaca
     7861 caacgtcaac cgattaccga aacgattgtt cgagatactc caaaaatcaa tcgaaacgat
     7921 accgtaacta tcaaacatgt aatgagtggt aaaacggaaa gtatgaaatt taaaaaagca
     7981 gaaagcatga ttgccagtgg tgattgggtt ttggtgaact aattttaaat aaatcaaata
     8041 atataatccc caatggttct aaactgttgg ggatttttta tatttgaaaa aaatcgaaca
     8101 atgaaaaaaa cagttatact attctttatt tttgtttcca ttcaaaccct ttattcgcaa
     8161 gaaaaaaata acagcccttt tttagaaccc aaaaatcaat ttaaagttga ggttgattcc
     8221 aaagaatatt tctattctga aggagatgaa ttagaaataa atcattccgc tacaaaatca
     8281 aaattaaaaa tcgaattact tggatttaaa aaattcgata gcggaaccgt tagttttcat
     8341 tatccgtcca actttgcttt tgaagtagac aaagattttg gttataataa ttggacattt
     8401 gacggcaata atgtagttat cttatatttt gaaattgata atgacatgaa aatgcaggaa
     8461 tatatagatt taatggttga acaattcggt aaaaaaaatt gcaaaactga aaaaaaacaa
     8521 attcaattag gaaacaaaac actttccggc acaagaatta atataacatt ggttggtcaa
     8581 ttattaagtc tggaattctt tgagattccg ttaaacgatt acaaaacccg ttttatagct
     8641 tttcaagatt ctaaagatga agaagggaac aattcaaaag aattagctga cacaataaaa
     8701 atggttcatg aaacgattac ttttttagag aattagatta aatgaaaaaa attataaatc
     8761 ttcttttaat tttaaatttt acagttttct attcacaatc catagaaaat ttaaaaattg
     8821 aaaccaaaaa tttctatgct gctcattaca acatggactt tgaaggaatt gcttctttgt
     8881 atcatccaaa atattttgaa aaattcacta aagaaacaat tactgccggt ttagatgaac
     8941 gctttcaaaa tgatgtggat ggaattcgtt ttgtttttcc gacaatgaca ttcaattatg
     9001 ctcctattca agaaattaat tccggtaaat attgtgtaat tacttttaaa aattcattta
     9061 gaataattaa gtctaatcca caagctgaga gtcaagtaca aaaagactta gcttcctatc
     9121 ttaaaattaa aaactataaa tcagtgaaat ttgaatctaa acgaaacagc tattttattg
     9181 aagaaaacgt tacttggatt gccgtttcat ctgctcaaac aagttggaaa tggacgttta
     9241 ttgaaatgaa ttcaaccttc aaatcgttag atgaattttt ggatacctca cttcaaaaag
     9301 aattaggttt ataattggtt taaaaaaata aatactatct ttactttgat gcttctaatg
     9361 gcattactaa acaagtatgg caaagagtca agatgcgaag aaaaacgtta agaaagagcc
     9421 tttaaaaacg gcgaaagaaa agaaagaaga gaaacgtgaa aagaaaaaca aaagggatta
     9481 attttttaat ccctttttgg ttgaatcaag gcaaaatctt tcccggattt aaaattccat
     9541 tcgggtcaaa aacaaatttt atccgttcca ttaattctaa atgcgtttta gaaaaagcaa
     9601 taggcatata atccttttga acatagccaa ttccgtgttc acctgaaagt gtccctttta
     9661 atgaaaccgt taattcaaaa atttccc
//
LOCUS       DAOTCW010000084         9632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_4768_length_9632_cov_38.928892, whole genome shotgun sequence.
ACCESSION   DAOTCW010000084 DAOTCW010000000
VERSION     DAOTCW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4768_length_9632_cov_38.928892"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="P5335_07755"
     CDS             complement(<1..1069)
                     /locus_tag="P5335_07755"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74809.1"
                     /translation="MFEWFYKLFVPTENPDITQSLVVVMIIIGIGVFVGRLKIGKVSL
                     GVSAVMFAGLFLGHFGYRINGDIMVFIRDLGLILFVYGIGIQVGPSFFSSFRKEGLKF
                     NILAVSTVLFGALIAVIIYLSTQIKIENVVGLLSGSVTNTPGLGAAKSVIQEYQNQFP
                     EKIFSDPTIAYAITYPMGVLGVILVIILSKAFLKIDVNKEKSNILQQESSEDVLIHQK
                     CRITKPEFIGKSIYQILKETDHDKIVVSRLKHSGSKTVFSPTLDTKVEHKDVIMLVGT
                     QHAINDFIEKTGKISTDLFIESEQDVISKSIFVTHKAATHKKLAELDLYNKYDLKVTR
                     VYRSGKELLASPSLELFYGDKL"
     gene            complement(1177..1665)
                     /locus_tag="P5335_07760"
     CDS             complement(1177..1665)
                     /locus_tag="P5335_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRZ74810.1"
                     /translation="MIGNDVVNLTLAKTESNWQRKGFLDKVFTKGEQNIIFGSENQDI
                     TVWTLWSRKEAVYKILRQKGESRGFYPLLINCSHENGLVHYKDSVFYSTTQIRNNCIH
                     TIAVTSITDFEYVTELNQLKESIYKNEIPFYLENGNCFFMSKSHHGRFERSIYLRKDI
                     TK"
     gene            complement(1662..1916)
                     /locus_tag="P5335_07765"
     CDS             complement(1662..1916)
                     /locus_tag="P5335_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRZ74811.1"
                     /translation="MNKAEIIEKLKALVKPYIKNQEAFDNLTETTDFITDLKINSANL
                     VDVILDIEEVFDIEIDNASMERMLNVKSAIEIIETKLAKK"
     gene            complement(1948..3219)
                     /locus_tag="P5335_07770"
     CDS             complement(1948..3219)
                     /locus_tag="P5335_07770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRZ74812.1"
                     /translation="MSKKRVVITGLGIVAPNGVGIDAFTNAIKNGISGIAHDPELERL
                     QFSCQIAGKPIISEELKNQYFTELELRNFNSSGILYGVIAGIEAWKNAGLPIQNNENP
                     DWDSGTIFGSGTSGIEKFRESIYKIDDFQTRRLGSTVVAQTMNSGISAYLAGKLGLGN
                     QVSTNSSACTTGTESILMGYDRIISGKANRMLIGSTSDSGPYIWAGFDAMKVCTFKHN
                     ESPEKGSRPMSATASGFVPGSGAGALILEDLETALARNATIYAEVLGGNVNSGGQRGE
                     GTMTAPNSVAVQRCIVEAMKNTGIKSMDIDSINGHLTATSKDSLEILNWSKALGLSGK
                     DFPPINSLKSMVGHCLSASGSMESVASILQIYHGFLFPNINCDDLHPAIIEFIDSSKI
                     PQQLIQKELNIIIKASFGFGDVNACVVFKKY"
     gene            complement(3221..3655)
                     /locus_tag="P5335_07775"
     CDS             complement(3221..3655)
                     /locus_tag="P5335_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HRZ74813.1"
                     /translation="MKNNYPEIFAKIPYSKPFIFVDEIHYVDENGIEGTYTFSEDLYF
                     YKGHFKNNPITPGVLLIETMAQIGLVCLGIYLTLEDNFTQIAFTSSEIEFLKPVYPNE
                     KVKVVSKKVYFRFGKLKCNVSMLNSKNVEVCSGTLSGMIIKP"
     gene            complement(3670..4452)
                     /locus_tag="P5335_07780"
     CDS             complement(3670..4452)
                     /locus_tag="P5335_07780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRZ74814.1"
                     /translation="MVSEFKNQWAIILGGSSGLGLATAKKLAQQGMNICIIHRNSRAE
                     LPKIEEDFEEIKALKISFLNFNLDATNIEKRIECVNEIQSKIGNQKIKCLVHSIAKGS
                     LKKMTGENSLTNDDFLITLNAMAISLYDWTKAVFEANLFDTDARIISFTSEGNTKPIQ
                     NYGAVSAAKVTLEAISRQIAIEFAAFGIRSNCIQAGVTNTTSFQMIPNSEKIKSFTEQ
                     RNPFKRLTKPEDVADVVYLLCKEEAKWITGTILKVDGGESLK"
     gene            complement(4446..5498)
                     /locus_tag="P5335_07785"
     CDS             complement(4446..5498)
                     /locus_tag="P5335_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HRZ74815.1"
                     /translation="MPVKIITVAKQLPKYSRETSEIVPFLDIWLDGQEERFIRKVKKI
                     FEGAQVDKRYSIMDPKEVFTLNSFEERNAIYSREMIVLGEQVLQKALKQANWSPTELD
                     YIITVSCTGIMIPSLDAYLINRLSLKQDIIRLPVTEMGCVGGVSGIIYAKNFLKANPN
                     KRAAVITVESPTATFQLDDFSMANIVSAAIFGDGAACVLLSSHENENGPTVVDEAMYH
                     FYDKEKMMGFDLTNNGLKMVLDIEVPETICEHFPAIIHPFLEKNNLSIADIHHLIFHP
                     GGKKIVLLVEELFKDLGKDIIDTKEILRLYGNMSSATVLYVLEQIMNKKPSKGEKGLM
                     LSFGPGFTAQRILLEW"
     gene            complement(5533..6243)
                     /locus_tag="P5335_07790"
     CDS             complement(5533..6243)
                     /locus_tag="P5335_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRZ74816.1"
                     /translation="MAIHTQYRTQAVEIMDDFSLEGEELREALDKIASINKLLGGNKL
                     TLQGIKKLLKETDETKTITIVDVGCGNGDMLRLMADFANKNNYTFQLIGIDANAFTIE
                     HARLLSKEYATISYRCEDIFSEAFTEVKYDILLCTLTLHHFKNEEIDTILNVFQKQAT
                     LGIVINDLHRSKLAYRLFQLVCSIFQLNRMSREDGLTSILRGFKKKELEDFSKKVQAK
                     RQTISWKWAFRYQWIIWK"
     gene            complement(6243..7361)
                     /locus_tag="P5335_07795"
     CDS             complement(6243..7361)
                     /locus_tag="P5335_07795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRZ74817.1"
                     /translation="MKESQVAIIGGGLAGLTAAIHLCQQGKNVVLFEKNSYPKHKVCG
                     EFISNEVLPYFNQLGLAIAHLNPTSINSTTISIASGQSISKDLPLGGFGISRYQLDNY
                     LYQEAIKWGCKIIHQNVNDVSFFDNEFNITTENQEVFKAKIVLGAFGKRSNLDIQLKR
                     DFIKQKSPWLAVKAHYSGDFPSDLVALHNFEGGYCGVSKVENDHINICYLVKFEVFKK
                     YKNIEEFQKNVLNQNPELKTIFEQSKLLFDNPLSISQISFEKKAQVENHLLMLGDSAG
                     LIHPLCGNGMAMAIHSAKIASECVISFLDNKLTREEMEKNYALQWNYYFKNRLQTGKQ
                     LSKLLLNQSMNRILMKILLTFPVLLTLIIRKTHGKPIS"
     gene            complement(7543..7899)
                     /locus_tag="P5335_07800"
     CDS             complement(7543..7899)
                     /locus_tag="P5335_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ74818.1"
                     /translation="MVNTSTATYEILPFLGDVINEMECDNSSYAVSKYGTLSLDLFNS
                     NKVLINPNPVSSRFQVSFEGNFGEYKIIDLLGKTIHSGMVFKDDFIDVSTLSNGIYIF
                     NLVHNGTTFTQKIVVN"
     gene            complement(8002..8469)
                     /locus_tag="P5335_07805"
     CDS             complement(8002..8469)
                     /locus_tag="P5335_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74819.1"
                     /translation="MINKITKKLFGSEVDMQTGFVYSYDGDIDNNIFLNKFDPISRIQ
                     TILGYFNFEEGTNFFPDSRCYDSNSKKYYFLILEGNQVKLVTASVASQPFSYTVIPLQ
                     NSEYSGNIGLEFSNETNTIFTVYSVYNQDNNVNDEIMIGTLNPNTGELTTMMT"
     gene            complement(8734..>9632)
                     /locus_tag="P5335_07810"
     CDS             complement(8734..>9632)
                     /locus_tag="P5335_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRZ74820.1"
                     /translation="LGNQLLITPLVQEISSTFPNCKIDLFLKGGLGPIIFKNYDNVEI
                     IIQLPKKHFKQLLLYIKSWSRIRKTKYDIAINVEKNSSSGRLSTKFANATYYFFGDNE
                     DDFRQKHDDYFHMAKYPVYSFREYITTIGLKAENNEVPYLNLKLSEDELIDGKSKLNT
                     LTQNNQQTICLFTYATGAKCYSSVWWSEFFENLKIKFPNKNIIEILPVENISMIDFKA
                     PTFYSKDIREMASLMANTNVFVGADSGIMHLASAAQVPVVGLFSVTNMEKYKPYNSNS
                     IGLNTDDSNNETIYKAIETILN"
BASE COUNT         3232 a         1668 c         1332 g         3400 t
ORIGIN      
        1 gcaatttatc gccataaaaa agttccaatg atggcgaggc taataattct tttcctgaac
       61 gataaactcg tgtaactttt aaatcatatt tgttataaag atctaattca gcgagttttt
      121 tatgagtagc cgctttatgg gttacaaaaa tgctttttga aattacatct tgttcagatt
      181 caatgaataa atcggtcgaa attttacctg ttttttcaat aaaatcattg atggcgtgtt
      241 gagtaccaac gagcattatc acatctttat gctcaacttt tgtatctaaa gtaggtgaaa
      301 aaacagtctt tgaaccactg tgttttaatc gagaaaccac tattttatca tgatcggttt
      361 cttttaaaat ttgataaata ctttttccaa taaattcagg ttttgtgatt ctgcattttt
      421 gatgaattaa aacatcttct gaactttcct gttgtagaat attagatttt tctttattaa
      481 cgtcaatttt taaaaatgct tttgatagta tgataactaa aatcacaccc aaaactccca
      541 ttggataagt tatagcataa gcaatggttg gatcactgaa tattttttca ggaaattgat
      601 tttgatattc ttgaattact gattttgcgg ctcctaaacc cggtgtattt gtgacagaac
      661 cggataataa accaactaca ttttcaattt ttatttgtgt acttaagtaa ataatgactg
      721 caattaaagc tccaaaaaga acagtggaaa cggctagtat attgaatttt aagccttctt
      781 ttctaaagga ggaaaaaaag gaagggccaa cttgaatccc aattccataa acaaacaaaa
      841 tcaaccctaa atcacgaata aaaaccatta tatcgccatt gattcggtag ccaaaatgcc
      901 ctaaaaataa cccggcaaac atcactgctg aaacacctaa tgatacttta ccaattttta
      961 atcggcctac aaaaacacca atcccaatga ttatcataac gaccactaac gattgagtaa
     1021 tgtcagggtt ttcagtggga acaaacaatt tataaaacca ttcaaacatg atagataatt
     1081 taatgatgta aaattaggta ttcgatttca gattttaatg atataaatca tgcaaatgaa
     1141 aacacaatgt attgaaaacg atatagtgaa taagttttat tttgtaatgt cctttcttaa
     1201 ataaatactt cgttcaaacc tcccgtgatg gcttttggac atgaaaaaac aatttccgtt
     1261 ttccaaataa aaaggtattt catttttata aatactttct tttagttgat ttaattcagt
     1321 gacatattca aaatcagtta tagaggttac ggcaatagtg tgaatacaat tatttctaat
     1381 ttgagtagtt gaataaaaaa cggaatcttt ataatgcacc aatccattct catgactaca
     1441 attaattaac aaaggataaa atccacggga ttcacctttt tgccggagta ttttataaac
     1501 agcttccttc ctactccaca atgtccaaac cgttatgtct tgattttcag aaccaaaaat
     1561 gatgttttgc tcgcctttgg taaatacttt atctaaaaaa ccttttcgct gccaattact
     1621 ttctgttttt gctaaagtta aattaaccac atcattgcca atcatttttt agctaattta
     1681 gtttcgatga tttctatggc tgattttaca ttaagcattc gttccatgga ggcattgtca
     1741 atctcaatat caaaaacttc ttcaatatct aaaattacat caaccagatt agcagaattt
     1801 atctttaaat cagttataaa atcagttgtt tcagtaagat tgtcaaaagc ttcctgattt
     1861 ttaatatagg gctttacaag agctttcaat ttttcaatta tttctgcttt gttcatgatt
     1921 taatttttga ctaagataag catctattta atacttttta aaaacaacac aggcatttac
     1981 atcgccaaaa ccaaaactgg cttttattat aatatttagt tctttttgta ttaattgttg
     2041 tggaatttta gaagaatcaa tgaattcaat tatggcagga tgtaaatcat cacaattgat
     2101 attcggaaat agaaatccat ggtaaatttg taaaatagaa gctacacttt ccatgctgcc
     2161 ggatgcagaa agacaatgac caaccattga ttttaatgag ttaattggtg gaaaatcttt
     2221 tcctgataaa cctaaagctt tactccagtt tagaatctcc aaactatctt tagatgtagc
     2281 tgttaaatgc ccatttatac tatcaatatc cattgacttt attcccgtat ttttcattgc
     2341 ttcaacgata catctttgaa cggctactga atttggagcg gtcatagttc cttcacctcg
     2401 ttgtccgccc gaatttacat ttccacctaa aacttcggca taaatggttg catttctagc
     2461 caaagcagtt tctaaatctt ctaaaatcaa agctcctgct ccacttcccg gaacaaatcc
     2521 tgaagcggta gcactcattg gtcttgagcc tttctctggc gattcattat gtttgaatgt
     2581 acaaactttc atagcatcaa atcctgccca aatatacgga ccgctgtcac tcgtgcttcc
     2641 tattaacatt cggttggctt tccctgaaat aattcggtca taacccatca atatactttc
     2701 tgttccggtt gtacaagctg atgaattcgt agaaacctgg tttcctaatc ctaatttacc
     2761 ggctaaataa gcacttatgc cactattcat tgtttgtgca acaacagtac tacctaacct
     2821 tctagtttga aaatcatcaa ttttataaat actttctctg aatttttcaa ttccggatgt
     2881 gcctgaacca aaaatagttc cgctatccca atccggattt tcgttatttt gaataggtaa
     2941 accggcgttt ttccatgcct cgattccggc gattacaccg tataagattc cactggaatt
     3001 aaaattgcga agttctaatt cagtaaaata ctgatttttc agttcttcag aaataattgg
     3061 tttacccgca atctgacacg aaaattgcaa tcgttctaat tctggatcat gagcaattcc
     3121 tgagattcca tttttaatgg cattggtaaa cgcatctatt cctactccat tgggagcaac
     3181 aattcctaat ccggtaataa caactcgctt ttttgacatt ttatggtttt ataatcatgc
     3241 cggataaagt tccgctacaa acttctacat ttttactatt caacatactc acattgcatt
     3301 ttaacttccc aaaacggaaa taaactttct tagaaacaac ttttactttt tcattaggat
     3361 aaacaggttt taaaaattca atttctgagg aagtaaaagc aatttgtgtg aaattatctt
     3421 ctaaagtcaa gtaaattcct aagcaaacta acccaatttg tgccatcgtt tctattaata
     3481 gtacaccggg agtaatcgga ttgtttttaa aatgcccttt ataaaaatat aagtcttcac
     3541 taaatgtgta agtaccctct attccatttt catcaacata gtgaatctca tccacaaata
     3601 taaaaggctt tgaataggga attttagcaa agatttcagg ataattattt ttcacacgta
     3661 aagcattaat tattttaaac tctctcctcc atctactttt aaaatggtgc ctgttatcca
     3721 tttggcctct tctttgcaaa gcaaataaac aacatcggcc acatcctccg gtttggtgag
     3781 tcgtttaaat ggatttcttt gttctgtaaa agatttaatc ttttcactat tgggaatcat
     3841 ctgaaaggaa gttgtattcg taacgccagc ttgaatacaa ttggaacgaa ttccaaaagc
     3901 agcaaattct atagctattt gtctcgaaat tgcctctaaa gtgacttttg cagcacttac
     3961 agctccgtaa ttttgaattg gttttgtgtt tccctcgcta gtaaatgaaa taattctggc
     4021 atcagtatca aataagttag cttcaaaaac cgcttttgtc caatcataaa gtgaaatagc
     4081 catcgcattt aaagttatta aaaaatcatc attcgtaagg gaattttcgc cggtcatctt
     4141 ctttaacgaa ccttttgcaa tgctgtgaac caagcatttt attttttgat ttccaatttt
     4201 agattgaatt tcattgacac attcaattct tttttcaata ttggttgcat ctaaattaaa
     4261 atttaaaaac gaaatcttca atgcttttat ctcttcaaaa tcttcttcta tttttggcaa
     4321 ttcagcacgt gaattgcgat gaataataca aatattcatc ccttgttgtg ctaatttttt
     4381 agctgtagct aaacctaaac cactactgcc acctaaaata attgcccatt gatttttaaa
     4441 ttcacttacc attctaataa tattcgttgt gcagtaaaac cgggtccaaa actcaacatt
     4501 aatcctttct ctcctttgga aggttttttg ttcataattt gttccaaaac atataatacg
     4561 gtagcactag acatatttcc gtacaaccgc agtatttctt tcgtgtctat aatatcttta
     4621 cctaaatctt taaataattc ttccactaaa agtacgattt ttttgccacc gggatgaaaa
     4681 attaaatgat gaatatcagc aattgataag ttattttttt ctaaaaaagg atgaataata
     4741 gcaggaaaat gttcgcaaat agtttcggga acttcaatgt ctaagaccat ttttaaacca
     4801 ttgttagtca aatcaaaacc catcattttt tctttatcat agaaatgata cattgcttca
     4861 tcaacaacag ttggaccatt ttcgttttca tgtgatgata acaaaacaca agctgcacca
     4921 tctccaaaaa ttgcagcact gacaatattg gccattgaaa aatcatccaa ttgaaaggtt
     4981 gcagttggac tttccacagt aataaccgcg gctcgtttat taggattcgc ttttaaaaag
     5041 ttttttgcat aaatgatacc tgaaacaccc cccacacaac ccatttcagt caccggaagc
     5101 cttataatat cttgcttaag cgataatcga ttaatcaaat aggcatctag tgaaggaatc
     5161 ataattccgg tacaacttac cgtgataata taatcgagtt cagttggact ccaattagct
     5221 tgttttaaag ctttttgtag tacttgttcg cctaatacaa tcatttcacg agaataaatg
     5281 gcattgcgtt cttcaaatga atttaaagta aaaacttctt tcggatccat aattgaatag
     5341 cgtttatcga cttgagctcc ttcaaatatt ttttttactt ttcgaataaa acgttcttcc
     5401 tgaccatcta gccaaatatc caaaaagggt actatttctg atgtctcacg agaatatttt
     5461 ggaagttgct ttgcaactgt aataattttt accggcattt attatatttt gaaaagtgaa
     5521 tagtttatta atttatttcc aaataatcca ttggtaacgg aaagcccatt tccatgaaat
     5581 tgtttgtctt tttgcttgta ctttttttga aaaatcttct aattctttct ttttaaatcc
     5641 acgtaatatg gaagttaatc cgtcttcgcg tgacatccta ttgagttgaa atatcgaaca
     5701 taccaattga aataaacgat aggcaagttt gcttctatgc aaatcattga ttacaatacc
     5761 aagagtcgct tgcttttgaa atacattcaa gatagtatca atttcttcat ttttaaaatg
     5821 atgtaatgtt aatgtacaca ataagatgtc atattttact tcagtaaatg cttcagaaaa
     5881 aatatcttca cagcgataac tgattgtggc atactcttta gacaataaac gggcatgttc
     5941 aatcgtaaaa gcattggcat ctattcctat gagttgaaaa gtataattat ttttattggc
     6001 aaaatctgcc atcaatcgaa gcatatcacc gttaccgcaa ccaacatcaa caatggtaat
     6061 agtttttgtc tcatcggttt ctttcaacaa ctttttaatg ccttgtaaag ttagcttgtt
     6121 tcctcccaac agtttattaa tagaggctat tttatccaaa gcttcacgta attcttcccc
     6181 ttctaaggaa aaatcatcca tgatttcgac tgcttgtgta cgatactggg tatgaatagc
     6241 catcaagaaa ttggttttcc gtgagtttta cgaatgatca aagtcaacaa cacaggaaat
     6301 gtaagcaata ttttcatcag tattcgattc atggattggt ttaacaataa ttttgacaat
     6361 tgtttacccg tttgtagcct attcttaaaa taataattcc attgaagggc ataatttttt
     6421 tccatttctt ctcgtgttag cttgttatct aaaaaggaaa taacacactc actggcaatt
     6481 ttagcactgt gaatagccat tgccattcca ttgccacaaa gcggatgaat tagtccggct
     6541 gagtctccta acatcaataa atgattttcc acttgtgctt ttttttcaaa tgaaatctga
     6601 ctaatgctta agggattatc aaacaataat ttcgattgtt caaaaatggt ttttagttcg
     6661 ggattttgat ttaatacatt cttttgaaat tcttcaatat tcttatactt tttaaatact
     6721 tcaaatttaa ctaaatagca aatattgatg tggtcgtttt caacttttga aacaccacaa
     6781 taaccgcctt caaaattatg taacgccact aaatcactag ggaaatcacc tgaataatgt
     6841 gcttttacag ccaaccaagg tgatttttgt ttaataaaat ctcttttcaa ttgaatgtct
     6901 aaattagaac gttttccaaa agctcctaaa acaattttag ctttaaaaac ttcttgattt
     6961 tctgtagtaa tattaaattc attatcaaaa aatgaaacat catttacatt ttgatgaatg
     7021 attttacatc cccatttaat ggcttcctga taaagataat tatctaattg atacctgctt
     7081 atcccaaatc cgcccaatgg caaatctttg gatattgatt gaccggatgc aatagaaatg
     7141 gtagttgaat tgatggatgt cgggtttaaa tgagcaatag ctaaccccaa ttgattaaaa
     7201 taaggtaata cttcattaga aataaattca ccacagactt tgtgtttggg ataactgttt
     7261 ttttcaaata gaacaacatt ttttccttgt tgacacaaat gaattgctgc agttaaaccg
     7321 gcaagacctc ctcctataat agctacttga ctctctttca tatcaaagta aatttaggaa
     7381 aattcaatga gaaaagtgtt gtatgattag attaaaaaat tataaagtta ttcataaaaa
     7441 aatgtaataa tactttatat tagaccgatt tttgaataga aatttatata gttaaattta
     7501 aaaataaaca actttttcag ttgcttaagc ctaagaaaaa aactaattaa caacaatttt
     7561 ttgagtaaaa gttgttccgt tatgaaccaa attaaaaatg taaattccat tagacaaagt
     7621 actgacatca ataaaatcat ccttaaaaac cattcctgag tgaattgttt tacctaacaa
     7681 atcaatgatt ttatattccc cgaagtttcc ttcaaaacta acttgaaaac gactacttac
     7741 cggattagga tttatcaata ctttatttga attaaataga tctaaggaca aagtaccgta
     7801 tttcgatacc gcatatgaag aattatcaca ctccatttca ttaataacat cacctagaaa
     7861 tggtaaaatt tcataagttg ctgttgaagt atttaccata tacaaacctg catcataatt
     7921 tgcatcaaaa ccaacaaata gtatggattc tgaatcttga tcataagttc tatttgaaaa
     7981 aagataacca agaatatttt cttaagtcat cattgtagtt aattcacctg tattcggatt
     8041 taaagtacca atcataattt catcattaac attattatct tgattataaa ctgaatacac
     8101 tgtaaaaata gtgttagttt cattgctgaa ttctagtccg atgtttccgc tatattcaga
     8161 attttgcaag ggaataacag tataagagaa tggttgtgac gcaacagaag ctgttactaa
     8221 tttaacttga tttccttcta aaattaaaaa ataatatttt ttcgaattac tgtcataaca
     8281 tctagaatca ggaaaaaaat ttgttccttc ttcaaaatta aaatagccta aaatggtttg
     8341 aattcgggat ataggatcaa atttatttag aaaaatattg ttatcaatgt caccatcata
     8401 agagtagaca aaaccagttt gcatatcaac ttcagaacca aacaactttt tagtaatttt
     8461 atttatcata acacccttaa ttttagatag tttaaacaaa aattggttta aaaaaatgta
     8521 aataagataa acaacagtta aaatacaagt tgttttcaac aaaacaaatt agatctcttt
     8581 atgttttaaa gtaaatttta tggttagtgg ttctcaaatt gaatcttgtc taatttgaca
     8641 aaaggtcata ttatattaga tttttttttc aattaaatta gaaatactaa tagatgagtt
     8701 cgcattcaaa gaagtatacc aaaactaact accttaattt aaaatagttt caattgcttt
     8761 ataaattgtc tcattattgg agtcatctgt attcagtcca atgctgttag agttgtatgg
     8821 tttatatttt tccatattag ttactgaaaa caaccccacg actggaactt gtgccgcact
     8881 ggctaaatgc ataattccgc tatctgcacc tacgaagaca ttcgtgttag ccatcaatga
     8941 agccatctcc cgaatatctt tactgtaaaa cgtaggagct ttgaaatcaa tcattgatat
     9001 attttcgaca ggtaaaatct caattatatt tttatttgga aacttaattt ttaaattttc
     9061 aaagaattca gaccaccaaa ctgatgaata acattttgct cctgtagcat aagtgaaaag
     9121 acaaatggtt tgttgattat tttgggttaa agtattgagt tttgatttgc cgtcaattag
     9181 ttcatcctca cttaatttta aatttaagta tggtacttca ttattttctg cctttagtcc
     9241 aatagtagtg atatattcac ggaaactata aacaggatat ttagccatgt gaaaataatc
     9301 atcatgtttt tgtctaaaat catcttcatt atcaccaaaa aaataataag tagcattagc
     9361 aaactttgta gaaagcctcc cggaagatga atttttttct acatttattg caatatcata
     9421 ctttgttttt ctaattctac tccaactttt tatataaagt aacaattgtt tgaaatgttt
     9481 ttttggtaat tgaattataa tttcaacatt atcataattt ttaaaaataa taggtcctaa
     9541 acctcctttt aaaaataaat cgattttaca gtttggaaaa gttgaggaga tttcttgaac
     9601 aagaggcgtt attaaaagtt gatttcctaa cc
//
LOCUS       DAOTCW010000085         9497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_4876_length_9497_cov_48.459754, whole genome shotgun sequence.
ACCESSION   DAOTCW010000085 DAOTCW010000000
VERSION     DAOTCW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4876_length_9497_cov_48.459754"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..264)
                     /locus_tag="P5335_07815"
     CDS             complement(70..264)
                     /locus_tag="P5335_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74821.1"
                     /translation="MNLKRIFGAILTVLGIAGLLYTAYLAVNSSESNQTLKTTIIYGV
                     LGLVFFIAGIGLVRTTKDES"
     gene            complement(575..2677)
                     /gene="feoB"
                     /locus_tag="P5335_07820"
     CDS             complement(575..2677)
                     /gene="feoB"
                     /locus_tag="P5335_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRZ74822.1"
                     /translation="MKENPIKVALIGNPNTGKTSLFNQLTGLNQQVGNYPGITVERKV
                     GSCWLPNNNKAVILDLPGTYSLNANSLDENLVIELLLNKNDADYPDVVVVVSEVENLK
                     RNLLLFTQIKDLEIPTILVINMADQMALKGISLDIPKLEAVLKTKVALISSRKATGIN
                     ELKELIVNFKKLSTEACLNASEIDKDYFENLRRIFPNQQQYKLWLVISQDVNFGSINK
                     AVLQNNSFSKPDSELKRLQHKETVKRYQFINDLLKEAKKVDLSKARDLGSKLDKMLTH
                     KIWGYVIFFGVLFLIFQSVFDWSSIPMDFIDEQFASLSSWTAENLPPGKLTDLIAQGI
                     IPGIGGVVIFIPQIAFLFLFISLLEESGYMSRVVFLMDKGMRRYGLSGKSVVPLISGT
                     ACAIPAIMATRNIENWKERLITILVTPFTTCSARIPVYTILISIIIPDQRLFGVLNLQ
                     GLTLMLLYALGFLMAIISAMILNKILQFKSKSFFVVEMPNYKIPYYKNVGLNVLEKTK
                     AFVFGAGKIIVSLSIVLWFMASYGPTANFKNAETIIQTEAKNTLTTDELEDKIASFKL
                     ENSYIGIIGKSIEPAIAPLGYDWKIGIGIVASFAAREVFVGTMATIYSVGSHEEEVNT
                     IKQRMRAEVHPETGKPIYNFASGISLLLFYAFAMQCMSTLAIVKKETNSWKWPIIQLL
                     VMSGFAYLVALLAFQLLK"
     gene            complement(2691..2924)
                     /locus_tag="P5335_07825"
     CDS             complement(2691..2924)
                     /locus_tag="P5335_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRZ74823.1"
                     /translation="MQTTVDLLKKGEKGVILSFDIDKIPLKLIEMGCMEGHLVELIQV
                     APFGDPLYLDMNGTHLAIRKEMAHEIVVEIIRN"
     gene            complement(3002..3673)
                     /locus_tag="P5335_07830"
     CDS             complement(3002..3673)
                     /locus_tag="P5335_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRZ74824.1"
                     /translation="MLSFIKRYKFLILGFVLFSVLFLSIAYQFLNPKKTLPIYNPSMV
                     NPELVDTTVQHISKYHTIADFSFTNQNGKTITEKDYEGKIYVADFFFTTCPTICPIMQ
                     DNMVEIQNAFKNNPNVMLLSHTVMPQIDSVPVLKKYALEKGVIDTKWNLVTGDKKDIF
                     YIARKSYLAVKTDTAGELYDMVHTENFILVDEQRRVRGFYDGTKNEDIKRLIEDIHFL
                     LGENK"
     gene            3787..7167
                     /locus_tag="P5335_07835"
     CDS             3787..7167
                     /locus_tag="P5335_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ74825.1"
                     /translation="MKKIILLLLFFQFSSSIAQFNSTAPWMDSVQKKETGDATIDEIK
                     AAFDKYWEGKNYKAKGSGYKPFMRWETHWRNKVDNSGHLLTPEQMWNAWNQKNQDRVN
                     RNSTLNAVSNWEPLGPFNHINTGSWSSGQGRVNVIVVDPNNANTLYLGAPAGGIWKST
                     DAGINWTPLSDYLPQIGVSGIAVDHSNSNVIYIATGDKDASDSYSIGVLKSIDGGITW
                     NTTGLSFTNTSTLASEIFMHPTNPDILWVATSVGLYKTTNAGVTWVQKLTGNVKDIKL
                     KPGNPDVIYAVTPNRFYRSTNGGDNFTQISSGMPTNSGRLIIDVTPANPEYVYVLSAR
                     TNWSHQGLYKSTNSGLTFAKTFNNTDIFEANQAWYDMALAVSDTNAEEVYTGVLNVWK
                     STNGGSSFMKLNNWSDPFQATYTHADIHFLRFYNGVLYCGSDGGIYVSSNSGESFTDL
                     TATAQIGQFYRISIAKQTSSKIAGGLQDNGGYAFSGNVWRNYYGADGMETAIDPNNSS
                     NFYGFIQNGSGLYFSTNSGNSLSGSINAPDGQNGNWITPLAINSLGELYAGFNGLYKL
                     NINAWTLISTTSFTSGNLERITIDPSNDNIIYVGDENNLFKSTDKGITFSLVDSFSNA
                     ITGIEVHSQDSNIVYVTTAGTSGSVFKSTTGGMGFLNIGAGLPNIGKNVIKHQGNHVD
                     NPLYVGTSLGVYYRDDTMSEWVPFDTNLPNVDVTDLEIHVLDEKIIASTYGRGVWQSS
                     IPTQLAQNDLALISLEEPSIENINCNVNSLSFLVKNTGLSTVTNFTLNYTINGTPNNF
                     IWNGNLESLATTTIEISGLSLIRGKSVIQGTVVLPNDSFANNNSINVEILNNDNGLVG
                     SLNTFETPFTNLVTSQPGGTSLWQRGIPTGTLLNTTSSGVRVYGTNLSGNYTDGTKAY
                     LYSQCYNFSQISNPVIRFKMAFDLELNWDILYLEYSTDIGQTWNVLGTAEDANWYNSS
                     RTPQTADDCNNCPGAQWTGTNSTMLEYSYMLNQFIGQSNIIFRFVFHSDEAVNNEGVI
                     IDDFIIDGTLSTSDFSLNEIAIYPNPSNGLFTLTYGNYKPQKVEVFDVTGKIILTIEE
                     NALSDDKIQLNLEKVTTGVYFVKISNEDQQVVKRIIKK"
     gene            7173..9215
                     /locus_tag="P5335_07840"
     CDS             7173..9215
                     /locus_tag="P5335_07840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HRZ74826.1"
                     /translation="MSRKNHIIGFFIFSISVCGYAQNESRGINLNFMDKSVKPSDNFF
                     RYVNGTWLDQTDIPADRTRWGSFDELREKTDKDMMLILKEALNNPTYTAETDQGKALN
                     LYRTIMDTVTRNKQGIKPLKPYLEKINAVKDVADLKKLLIEMETQGGVGFFGVGVGTD
                     AKNSNRNVVNVGLGSLGLPDRDYYVSEDKDSKEKREKYHAHIIKMMSYLGEKPAQAQA
                     TADRVLTLEIAMATPRLDRVERRDRRKTYNPMTIAELQNLTPTINWNEYLQGVGLPIV
                     DTIIVSQPRYMTALETIFKENKVEDWKAYMRWTLLNRYAGLLSTEIGNANWEFYGKTL
                     TGAIKQRPFDERALQSVNGTFGEALGKIYVEKMFPPEAKEKARKMIDNLFLAYENRIN
                     NLPWMAKSTKDNAIAKLKKFNVKIGYPDKWKDYSKVTIIGPKEGGTYFDNSKRITKWR
                     NDENFAKLNKPVDKTEWGMSPQTVNAYYNASFNEIVFPAAILQPPFYDYRADEAVNYG
                     GIGAVIGHEISHGFDDSGSRYNADGNLVNWWSEEDLKQFTELGGALADQYSQLEPLPG
                     IFVDGKFTLGENIGDLGGVSAALDGLKIHLKQAGNPGLIDGYTPEQRFFISWATIWRS
                     KARDEAIKNLVKTDSHSPGQYRAYVPLQNVDAFYDAFSIGKGDGMYLEEDKRVKIW"
BASE COUNT         3285 a         1490 c         1692 g         3030 t
ORIGIN      
        1 caaaatttat caattaattt ataaccaatt gaaaatgaaa aatttaatta aaatagaaaa
       61 atcaattgat tatgattcgt cttttgttgt gcgaactagt ccaattccgg caataaagaa
      121 taccaaacct aaaacaccat aaataatagt cgttttaaga gtttgattgc tttcggagga
      181 gtttaccgct aaataagcag tatataatag accagcaata cctaaaacgg ttaaaattgc
      241 gccaaaaatt cgtttaagat tcataaagac gtaaatttaa ttagacagtt gttttactta
      301 agacatcaaa aattccgaag actttcaata accaaatgac aacccctatg actacaatta
      361 aattaaaaat acttttaatt tttcggtcca tcgggatata attatttatt atccaaagta
      421 ggaatccaac aactataagg acgagtaaaa tgtttaaaat tggcataagt gtatctatta
      481 agaataacca aaggtaagtc attgaaaatc catcaaatta cacaacttta agtaaaagtt
      541 gcaaaattca tacgttgaaa cttgaaaaaa ggagttattt taataactga aatgccaaca
      601 aagctaccaa ataggcaaaa ccactcatca ctaaaagctg aatgattggc catttccaag
      661 aatttgtttc tttttttacg atagcaagtg tactcatgca ctgcattgca aatgcataaa
      721 aaagcaataa agaaattccg gaagcaaagt tataaatagg tttccctgtt tcaggatgca
      781 cttctgcccg catacgttgt ttaattgtgt tcacttcttc ctcatggcta ccaacgctgt
      841 aaatggttgc cattgttccc acaaacactt ctcgtgcagc aaaagaagca acaattccga
      901 ttcctatttt ccaatcgtag cccaaaggag caatggcagg ttcaatggat ttgccaataa
      961 taccaatata ggagttttct aatttaaacg aagcaatttt atcttccaat tcatctgtcg
     1021 ttagtgtgtt ttttgcttct gtttgaatga ttgtttctgc gtttttaaaa ttagctgttg
     1081 gtccataaga agccatgaac cacaaaacaa tggatagcga aacaattatt ttacctgcac
     1141 caaatacaaa tgcttttgtt ttttcgagta catttaaacc tacattttta taataaggaa
     1201 ttttgtagtt aggcatttct accacaaaaa aacttttgga tttgaattgc aaaattttat
     1261 tcagaatcat agctgagatt atggccatta aaaatccaag tgcatacaaa agcattaaag
     1321 tcaagccttg cagatttaaa acgccaaata atcgttgatc gggtatgatg atagaaatca
     1381 aaattgtata aaccggaatt cgtgccgaac aagtggtaaa tggtgtaact aaaatagtta
     1441 ttaaacgctc tttccaattt tctatgtttc gggttgccat aattgcagga atggcacaag
     1501 cagttcctga gattaatgga accacacttt ttccgctcaa accatatctt cgcatgcctt
     1561 tatccattaa aaagacaaca cgactcatat agccgctttc ttccagcaag gatatgaaaa
     1621 gaaaaagaaa ggcaatttga gggataaaaa taaccacacc accaatacca ggaattattc
     1681 cttgagcaat caaatctgta agttttccgg gtggaagatt ttcagcagtc caggaactta
     1741 aagaggcaaa ttgttcgtca ataaaatcca ttggaatact cgaccaatca aaaacagatt
     1801 gaaaaatcag aaataaaaca ccaaagaaaa ttacatatcc ccaaatttta tgggttagca
     1861 ttttgtctaa tttacttcct aaatcgcgag ctttagacaa gtcaactttt tttgcttctt
     1921 ttaataaatc atttataaat tggtaccgtt tgaccgtttc tttatgttgc aaacgtttca
     1981 attccgaatc aggttttgaa aatgaattgt tttgcaaaac cgccttattt atggagccaa
     2041 aattaacatc ttgcgaaatg accaaccaca atttgtattg ttgttgattc gggaaaatcc
     2101 gacgtagatt ttcaaaatag tctttgtcaa tttcagaggc attcaaacaa gcttccgtcg
     2161 ataatttttt gaaattgaca attaattctt ttaattcatt gattccggtt gcttttcggg
     2221 aactgattaa ggcaacttta gtttttaaaa cggcttccaa ttttggtata tccaatgaaa
     2281 tacccttcaa cgccatttga tcggccatgt taatgaccaa aatcgtggga atttctaaat
     2341 cttttatttg agtgaaaagc agcagatttc gtttcaaatt ttctacttct gaaacaacca
     2401 ccaccacatc cggataatca gcatcgtttt tatttaacaa caattctata accagatttt
     2461 catctaatga atttgcattc agactatacg ttcccggtaa atcaagtata acagctttat
     2521 tgttattagg taaccaacaa cttcctactt ttcgttcaac cgtaattcct ggatagttac
     2581 ccacctgttg atttaatccg gtgagttgat taaataatga agttttaccc gtgtttgggt
     2641 taccaataag tgcaacttta atggggtttt ctttcatgtt tttgttgcaa ttaatttcga
     2701 atgatttcta caacaatttc atgtgccatt tcttttcgaa tagccaaatg agttccattc
     2761 atatctaagt acagcgggtc tccaaatggt gcaacttgaa ttaattcaac taaatgtcct
     2821 tccatacaac ccatttcaat taatttcaat ggaattttat cgatatcgaa gcttaaaatc
     2881 actcctttct cgcctttttt gagcaggtca acagttgttt gcaatgttta tttagaataa
     2941 atataaatac aaaagtacag ttttttataa aacctttact gataaatgtc agtttataag
     3001 tttatttatt ttcgcccaat aaaaaatgaa tatcttcaat taacctttta atatcttcat
     3061 ttttagtgcc atcataaaaa ccacgaactc ttctttgttc gtcaactaga ataaaatttt
     3121 ccgtatgaac catatcgtat aactccccgg ccgtatctgt ttttactgct aagtacgatt
     3181 ttcgggctat atagaaaata tcttttttat ctccggttac taagttccat tttgtgtcaa
     3241 ttacaccttt ttctaaagca tattttttca ataccggaac actgtcaatt tgaggcataa
     3301 cggtgtgaga aagcaacatt acatttggat tatttttaaa tgcattttga atttcaacca
     3361 tattatcttg catgattgga caaatggtgg gacaagtggt aaagaaaaaa tcagctacat
     3421 aaatttttcc ttcatagtct ttttcagtaa ttgtttttcc attttgatta gtgaatgaaa
     3481 aatcagcaat ggtatgatat ttactgatat gttgcacggt agtatctact aattcgggat
     3541 ttaccatcga aggattataa atgggtaatg ttttttttgg atttaaaaat tgatatgcta
     3601 tcgaaagaaa tagtacagaa aataagacaa atcctaagat taaaaattta tatcgtttga
     3661 taaaagagag catactgttt ttttgcaaag gtaaaaaaga taacaaacta aatactagtt
     3721 atacaagtgt taattgtgct agtaaattca attggttttt atatttttga ataaaattta
     3781 aaataaatga aaaaaattat actcctttta ttgttttttc aattttcatc ttcaattgct
     3841 caatttaatt caacggcacc ttggatggat tctgttcaaa agaaagaaac tggtgatgca
     3901 acaattgatg aaattaaagc tgcatttgat aagtattggg aaggtaaaaa ttataaagca
     3961 aagggaagtg gctacaaacc tttcatgcgt tgggaaaccc attggagaaa taaagtggat
     4021 aattcgggtc atttattgac tcctgaacaa atgtggaatg cttggaatca aaaaaatcaa
     4081 gatagggtta atagaaattc aacgcttaat gctgttagta attgggaacc actaggacca
     4141 tttaatcata tcaacacggg ctcttggtca tcggggcaag gaagggtaaa cgttattgtg
     4201 gtcgatccaa ataatgcaaa tacactttat cttggtgctc cggcaggagg aatatggaaa
     4261 tcaacagatg caggtataaa ttggactcca ctttctgatt atctccctca aattggagtt
     4321 tcgggtatag cagttgatca ttcaaattca aatgttattt atattgctac cggagataaa
     4381 gacgctagtg attcttattc aataggagtt ttaaaatcaa ttgatggtgg aattacatgg
     4441 aatactaccg gactttcctt tactaacact tctactttag caagtgaaat ttttatgcat
     4501 ccaaccaatc ctgatatatt atgggttgcc acaagtgtgg gcttgtataa aaccacgaac
     4561 gcaggggtta catgggttca aaaattaact ggaaatgtta aagatataaa gttaaaacca
     4621 ggaaatccag atgtgattta tgcggttact ccgaatagat tttatcgttc aacaaatgga
     4681 ggggataact tcacgcaaat tagttcagga atgcctacga attcaggaag attaattatt
     4741 gatgtaactc cggctaatcc ggaatatgta tacgtattga gtgctcgaac aaattggagt
     4801 catcaagggc tttacaaatc aacaaattca ggattaacat ttgcaaaaac gtttaacaac
     4861 acggatattt ttgaagctaa tcaggcttgg tatgatatgg cattggcagt ttcagacact
     4921 aatgccgagg aagtttatac aggggtttta aatgtttgga aatcaacaaa tggaggaagt
     4981 tcttttatga aattaaataa ttggtcagat ccttttcagg ctacttatac tcacgcagat
     5041 atacattttt taagatttta caatggagta ctttattgcg gaagcgatgg aggaatttat
     5101 gtttcttcaa atagtgggga atcatttact gatttaacag ctaccgctca aataggacaa
     5161 ttttacagaa tttcgattgc aaaacaaact tcttctaaaa tagccggagg attacaagat
     5221 aatggaggtt atgcctttag tggtaacgtt tggagaaatt attatggagc agatggaatg
     5281 gaaacggcca tagatccaaa taacagtagt aatttttatg ggtttattca aaacggaagt
     5341 ggattgtatt tttcaacaaa ttcaggaaat agtttatcag gaagtattaa tgctccggat
     5401 ggacaaaacg gaaattggat aactccttta gctattaatt ctttggggga actatatgct
     5461 ggttttaacg gactttataa gttgaatatc aatgcttgga ctttgatttc tactacttca
     5521 tttacatccg gtaatttaga aagaattacc attgatcctt ctaatgataa tattatatat
     5581 gtgggagatg agaataattt gtttaaaagt acagacaaag gaataacatt ttcattagtg
     5641 gattcctttt caaatgcgat aacagggata gaggttcatt cccaagatag taatattgtt
     5701 tatgtaacca cggctggaac ttcaggaagt gtttttaaat ctacaaccgg aggaatgggc
     5761 tttttaaata ttggagcagg actgcccaat atcggtaaaa atgtaatcaa acaccaagga
     5821 aatcatgttg ataatccatt atatgtagga acaagtttag gggtttatta tcgggacgat
     5881 accatgagtg agtgggttcc ttttgacact aaccttccaa atgttgatgt caccgattta
     5941 gaaattcacg tattggatga aaaaattatt gcttcaactt acggaagagg tgtttggcaa
     6001 tcatcaattc caacacaatt agcccaaaat gatttggctc tgattagttt ggaagaaccc
     6061 tctattgaaa acataaattg taatgtcaat tcattatcct tcttagttaa aaatacagga
     6121 ttatcaacgg ttactaattt tacgcttaat tacactataa atggaactcc aaataatttt
     6181 atttggaatg gaaatctgga gtctttggca acaacaacta ttgaaatttc cggacttagt
     6241 ttaattagag ggaaatcagt gattcaagga acagttgttt tgcctaatga ttcatttgcc
     6301 aataataaca gtatcaatgt tgaaatttta aacaatgata atggcttagt aggttcttta
     6361 aacacctttg aaacgccatt tacaaacctt gtaacttcac aacccggagg aactagtttg
     6421 tggcaaagag gaataccaac aggaacatta ttaaatacaa cttcttcagg agtgagagtt
     6481 tatggaacga atttgagtgg taattataca gatggaacaa aagcttattt atattctcaa
     6541 tgttataatt tctctcagat ttctaatcct gtcatccgat ttaaaatggc ttttgattta
     6601 gagttgaatt gggacatttt atatttagaa tattctactg acataggaca aacttggaat
     6661 gttttaggaa cagctgagga tgctaattgg tacaatagta gtcgaactcc acaaaccgct
     6721 gatgattgta acaattgtcc cggagcacaa tggacaggaa ctaattccac tatgcttgaa
     6781 tatagctata tgttgaatca gtttattggt caaagtaaca taatttttag atttgttttt
     6841 cattcagacg aagcggttaa taatgaagga gtcattattg atgattttat aatcgatggt
     6901 acattgtcta catctgattt tagtttaaat gagattgcta tttatcctaa tccgtctaat
     6961 ggattgttta cacttactta tggtaattat aaaccgcaaa aagtggaagt atttgatgta
     7021 acaggtaaaa tcattttaac cattgaggaa aacgctttat cagatgataa aattcaattg
     7081 aaccttgaaa aagtgacaac gggtgtttat tttgttaaaa taagtaatga agatcaacaa
     7141 gtagttaaaa ggataattaa aaagtaaatt taatgagtag aaaaaatcac atcatcggat
     7201 tttttatttt tagcatcagc gtttgcggat atgcccaaaa tgaatcacgc ggaattaacc
     7261 tgaatttcat ggataaatca gttaagccaa gtgataattt ctttcgctat gtcaatggaa
     7321 cttggctcga tcaaacagac attcctgctg atagaacccg atgggggagt tttgatgaac
     7381 ttcgtgaaaa aacagataaa gatatgatgc ttattttaaa agaagcattg aataatccta
     7441 cttatactgc cgaaacagat caagggaaag cactaaatct ataccgtaca attatggata
     7501 cggttactag aaataaacaa ggaattaaac cgttaaaacc ttatctagaa aaaattaatg
     7561 ccgtaaaaga tgtagctgac ctgaaaaaac tcttaataga aatggaaact cagggaggag
     7621 ttggtttttt tggagttggc gtaggaacag atgctaaaaa cagcaataga aatgtggtaa
     7681 atgttggttt aggaagtctt ggcttaccgg atcgtgatta ctatgtttcc gaggataaag
     7741 acagtaaaga aaaaagagaa aagtatcatg ctcacattat caaaatgatg agctatttag
     7801 gcgaaaaacc ggctcaagct caagctactg ctgatagagt tttaactctc gaaattgcca
     7861 tggcaacacc acgtttagat cgagtggaac gaagagaccg aagaaaaact tacaatccga
     7921 tgacgattgc tgaacttcaa aatttaacgc ctaccattaa ttggaatgaa tatttgcaag
     7981 gagttggttt accaatagtg gatacaatta tcgtttctca accgcgttac atgaccgcat
     8041 tagaaactat tttcaaagaa aacaaagtgg aggattggaa agcctacatg cgttggacac
     8101 ttcttaatcg ttatgccgga ttattatcaa cagaaattgg aaatgccaat tgggaatttt
     8161 atggaaaaac cttaacggga gccatcaaac aaagaccgtt tgatgaaaga gcattacagt
     8221 ccgttaacgg aacctttgga gaagcattag gtaaaattta tgtagaaaaa atgttcccgc
     8281 cagaagcaaa agaaaaagcc agaaaaatga ttgataattt gtttttagcg tatgaaaaca
     8341 gaatcaataa tttaccttgg atggcaaaat caactaaaga taatgccatt gcaaaactca
     8401 aaaaattcaa tgttaaaata ggctatcctg ataaatggaa agattattct aaagttacaa
     8461 tcattggacc aaaagaagga ggaacttatt ttgataactc caaaagaata acgaaatggc
     8521 gtaatgatga aaatttcgcc aaattaaata aaccggttga taaaacagaa tggggaatgt
     8581 caccacaaac ggtgaatgct tattataatg catcatttaa tgaaattgtt tttcctgcgg
     8641 ctattttaca accgccattt tatgattaca gagctgacga agcagttaat tatggcggaa
     8701 ttggagctgt tattggacat gaaatatcac acggatttga tgatagcggt tctcgttaca
     8761 atgccgatgg aaatttagta aattggtgga gtgaagaaga tttgaaacag ttcacagaac
     8821 tgggcggagc attagcggat caatattcgc agttagaacc actaccggga atttttgtgg
     8881 acggaaaatt tactttaggt gaaaatattg gtgatttagg cggagtaagt gcagctctgg
     8941 atggtttaaa aattcattta aaacaagctg gcaatccggg attaattgac ggatatactc
     9001 ctgaacaacg tttctttatt tcttgggcta ctatttggag aagcaaagcc agagatgaag
     9061 ccattaaaaa tttagtaaaa acagattctc attcaccagg acaatacaga gcttatgttc
     9121 ccctgcaaaa tgttgatgca ttttatgatg ccttctccat tggaaaggga gatggaatgt
     9181 atctggaaga agataaaaga gttaaaattt ggtaaaagag ttataaattt gataatcaaa
     9241 acaataaaac aaataatagg ccgttgtaac tttgtaatca ttgcataatg caagttagag
     9301 ttagttaaat tttgttttgg tgttttgaga aaggcgttcc tgaatttgat tcgggaacgc
     9361 cttttgattt tcttctgagt tgctgagaag ctgagtctct aagctgctga gatttatatt
     9421 gatttttctc agtttctcag ctactttcta ataactcatt tctacaattt gtttcacttt
     9481 ttctaacgta atattct
//
LOCUS       DAOTCW010000086         9368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_4975_length_9368_cov_51.338559, whole genome shotgun sequence.
ACCESSION   DAOTCW010000086 DAOTCW010000000
VERSION     DAOTCW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4975_length_9368_cov_51.338559"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="P5335_07845"
     CDS             <1..963
                     /locus_tag="P5335_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRZ74827.1"
                     /translation="EKVSEETLKNVKAGYIGKFVMDIEKPATVARYALQTKTQGLPAD
                     FYENYIKNINAVTADDILRVSKKYFLAENLRIVVVSKGTDVLPGLEKLNIPIFYFDKY
                     GNKVEKPVAKTVSADVSAKSVLDKYIAAIGGQKAVSGVKSIMTTSSGTVQGMALEMTT
                     KVTSSNKLAVEMKAMGTTMMKQVVNENGAYMVQQGQRKDFTGDDLKEMQAGAVPFEEL
                     TLANNKDITLGAVENINGTDAYAVKNGKSTYYFDVKTGLKIAEAKELEQGGQKMTQMT
                     YYSDYKEVKGIKVPHKTTLNIGVELELTTSDVKINEGVTDEDFK"
     gene            complement(1013..1879)
                     /locus_tag="P5335_07850"
     CDS             complement(1013..1879)
                     /locus_tag="P5335_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRZ74828.1"
                     /translation="MENKNLRFVYLIVLALIWGSSFILIKRGLVALTPYQLGSLRMVF
                     AAVFLLIIGFNSIKNIELRHWKYLALTAVFGTFIPVFLFSIAQTQISSSVSAILNSLT
                     PLNTLVLGIILFKIDFQRRQIFGVFIGLMGSALLIFNGAANHPEQNYNYAVLLVIASV
                     CYAMNVNFIKMYLSDLNPLSITTGNFIIMLIPASLVLYFSGFFEAVQVKETQNVIGYI
                     AVLGIVGTALANILFYKLIKISSPVFASSVTYLIPVVAFMWGLVDNESLTLVQFIGAA
                     IILVGVYLSARK"
     gene            2000..2500
                     /locus_tag="P5335_07855"
     CDS             2000..2500
                     /locus_tag="P5335_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRZ74829.1"
                     /translation="MKFTKKIALFAFTILSIVSCKKEATETATTEQKQEQPAQLATAS
                     FTIEGMSCEVGCANHLEKKLAKLDGVQKAAVDFEAKKATVEYDANKVTPEVLVQTVEG
                     AADGKTYKVSDMTNSADHALLFTKEKKKKKTADSKKENASSEEKKGCEPEKKSGCCSA
                     KKVETL"
     assembly_gap    2609..2708
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2709..2941)
                     /locus_tag="P5335_07860"
     CDS             complement(<2709..2941)
                     /locus_tag="P5335_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HRZ74830.1"
                     /translation="MRKKSLYFALFMLISFFMACKEEVLPKPTAQLRLDYQLANYMDF
                     DSPCPFNFDYNIDTKVKVKEDCGVTIEYPKMKAT"
     gene            complement(2934..4229)
                     /locus_tag="P5335_07865"
     CDS             complement(2934..4229)
                     /locus_tag="P5335_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HRZ74831.1"
                     /translation="MDSEPSSLLENIDTSLLLGILGILVLLLFSALISGSEVALFSLS
                     NKDIDEATAQNQSKGNIISTLLSKPKKLLATLLIANNFINIGIVIIFSFIKEDLFSQI
                     KFDWLRFAIEVGVVTFLILFFGEVLPKVYANRNNVKFAKKIASPIAILNMLLTPINMP
                     MRAITIYLENKLGKQKSNISVNQLSQALELTSSEDTSQEEQKILEGIVSFGNTDTSQV
                     MSPRIDLFALEIEESFTEVIIKILDKGYSRIPVYRDNIDQIEGVLYVKDLLPHIDKKE
                     FDWTTLLREPLFVPENKKLDNLLKDFQSMKNHLAIVVDEYGGTSGVITLEDIIEEIVG
                     DISDEFDDENINYSQIDDKNFLFEGKINLKDFYRIVEVDEDLFENNKGEAETLAGFIL
                     EIIGTFPRKGQKITFDKCLFTIESVDKKRVKQIKVTLDA"
     gene            complement(4252..4698)
                     /gene="ssb"
                     /locus_tag="P5335_07870"
     CDS             complement(4252..4698)
                     /gene="ssb"
                     /locus_tag="P5335_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRZ74832.1"
                     /translation="MNGTLNKVMLIGHLGDDVKMHYFDGGNCIGRFPLATNEVYINKT
                     TNEKITSTEWHNLVVRNKAAEICEKYLSKGDKIYVEGRIKSRQWQAEDGQTKYTTEIQ
                     VTEFTFLTTKKETELNKQQVPSMTEPNKNTNFEAQRGSGNQDDLPY"
     gene            complement(4749..5786)
                     /gene="mutY"
                     /locus_tag="P5335_07875"
     CDS             complement(4749..5786)
                     /gene="mutY"
                     /locus_tag="P5335_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRZ74833.1"
                     /translation="MTFSNRLISWYLENKRDLPWRNTTNPYKIWLSEIILQQTRVAQG
                     LPYYIAFVETFPTVFDLANASEEQVLKLWQGLGYYSRARNLHKTAQLVAFDLGGNFPT
                     SYQELLKLKGIGTYTAAAIASFSSNEPVPVVDGNVFRVLARYFDVETDISSTIAKKEF
                     TALAYEVLDKTNSLQFNQAIMEFGALQCMPKNPNCSVCVLNDSCVALQKNKINQLPVK
                     LKKTKVTERFFNYLVFEDELGNSIIQQRTEKGIWHNLYEFPLIESFIEITEKEITDEI
                     NKMKFFSNEIMSINAYEEKSVLHILSHQRLKIRFWNIQLKGKLENAKSKQSIKKRPFP
                     IVIFNFIEKYF"
     gene            5920..6213
                     /locus_tag="P5335_07880"
     CDS             5920..6213
                     /locus_tag="P5335_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRZ74834.1"
                     /translation="MTKADIVAKISEKLGLEKGDVQATVESFMEEVKNSLETGDNVYL
                     RGFGSFIIKTRAEKTGRNISKNTTIKIPAHNIPAFKPAKVFVEGVKTNTEVAV"
     gene            6554..8098
                     /locus_tag="P5335_07885"
     CDS             6554..8098
                     /locus_tag="P5335_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRZ74835.1"
                     /translation="MNKELIIRSGSEAVDFALLKDGKLVELHKEEEQKSGFQVGDIFI
                     AKIRKPVPGLNAAFVHLGFEKDAFLHYHDLGPNLSSMLKFTKLVSTGKQKDFSLKNFQ
                     FEKEIDKDGTITDVLSANQSVLVQIVKEPISTKGPRISSELSLAGRYIVLVPFSDRVS
                     VSQKIESKEEKDRLKKLVQSIKPKGFGVIVRTVAEGKKIAELDQDMQNLLSKWTAMCK
                     KLPTAHHPSKVLSEMNRASSILRDVFNDSFTGIYIDDEELFEQTKESIEEIDPSKVNI
                     VKLYQSKELPIFEKYHIERQIKTSFGRTVSMSKGAYLIIEHTEALHVIDVNSGNRSNK
                     AQNQEDTALEVNMIAAAEVARQLRLRDMGGIIVVDFIDMQNPENRKVLFDFLREEMSD
                     DKAKHKILPPSKFGLIQITRQRVRPEVNIKTREEDPNNGTGEIDAPIQVIDKINLDVE
                     RLKNHKKLVLNAHPFVAAYLTKGFPSLRSKWFFEHKKWVKIIPRDAYTFLQYQFYDKE
                     GNVIKE"
     gene            8223..9179
                     /locus_tag="P5335_07890"
     CDS             8223..9179
                     /locus_tag="P5335_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HRZ74836.1"
                     /translation="MKFYLFSCFFCWIIFQNTSSAQLGCTDVLARNYNQNAVENDGNC
                     CYKRLKIKKFTSVVLNDMLNETSSLVEWENNLYTHNDDTDLHLYQLDKNGSITKRIKL
                     NGIKNNDWEELTQDETHFYLGDFGNNASGNRSDLTIFKIAKNSLFDNPKIETISFSYP
                     EQTDFSKQKSNTTNFDCEAFIATENELILFTKQWKNNQSAVYRLPKTAGKYSAEYITT
                     LKVNGLITGATFVEVKNLITLCGHTKKLKPFMYLIYDLKNVNFENINQRKIKLKLPFH
                     QIEAISSINGIDFYITNERFSRKIIGTVLQQLHHFSLEFMSR"
     gene            complement(9189..>9368)
                     /locus_tag="P5335_07895"
     CDS             complement(9189..>9368)
                     /locus_tag="P5335_07895"
                     /inference="COORDINATES: protein motif:HMM:NF018534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74837.1"
                     /translation="KVAHSQPNIKLYWYVDNTYLGITQNFHEMPLIVASGIHYVTVVD
                     EKGNEIKRKVEVEQE"
BASE COUNT         3361 a         1572 c         1461 g         2874 t
ORIGIN      
        1 gaaaaagtat ctgaagaaac attaaaaaat gtaaaagcag gatacattgg aaaatttgta
       61 atggatattg aaaaaccggc tactgttgcc cgttatgctt tacaaacaaa aacacaagga
      121 ttaccggctg atttttacga aaattacatt aaaaacatca atgcagttac tgctgacgac
      181 attttaagag tctctaaaaa atatttctta gctgaaaatt taagaatcgt agtagtgagt
      241 aaaggaactg atgttcttcc cggtttagaa aaactgaata ttccaatttt ctattttgat
      301 aaatacggaa ataaagttga aaaacctgta gcaaaaacgg ttagtgcaga tgttagtgca
      361 aaatcagttt tagacaaata catagccgct atcggtggac aaaaagcagt ttctggtgtg
      421 aaaagcatta tgactacttc ctccggaaca gtacaaggta tggctttgga aatgactact
      481 aaggtaacat ccagtaataa attggctgtg gaaatgaagg caatggggac taccatgatg
      541 aaacaagtag taaatgaaaa tggtgcttat atggttcaac aaggccaacg aaaagatttt
      601 accggtgatg atttaaaaga aatgcaagca ggtgccgttc catttgaaga attaacatta
      661 gctaacaaca aagacattac attaggtgca gttgaaaaca tcaatggaac agacgcttat
      721 gctgttaaaa acggaaaatc tacttattat tttgatgtga aaaccggatt aaaaattgca
      781 gaagccaaag aattagaaca aggaggccaa aaaatgactc aaatgaccta ttattccgac
      841 tataaagaag tgaaaggaat taaagtccct cataaaacca ctttgaatat tggtgttgaa
      901 ctagaattga caacttctga tgtcaaaatc aatgaaggtg taaccgacga agattttaaa
      961 taaaaaacaa cttttcagtg ataaaaggac tgtcagaaat ggcggtcctt ttttattttc
     1021 tagccgataa ataaactccg acaagaataa tcgcagctcc tataaattga actaacgtca
     1081 agctttcatt gtcaaccaat ccccacataa aagcaacaac cgggattaaa taagtaaccg
     1141 aagaagcaaa aacaggagac gaaattttta ttaatttata aaataagata ttcgccaaag
     1201 ctgttcctac aattcctaaa acagcaatat aaccaattac attttgcgtt tcttttactt
     1261 gtactgcttc aaaaaaccct gaaaaataca atacaagaga agccggtatc aacataataa
     1321 taaaatttcc ggttgtaatg ctcaacggat ttaaatcgga taaatacatt ttaataaaat
     1381 tgacattcat tgcataacag acagaagcaa taactaacaa taccgcataa ttataattct
     1441 gttccggatg attagcggct ccattaaaaa taagcaatgc acttcccatc aaaccgatga
     1501 aaacgccaaa gatttgtcgg cgttgaaaat ctattttaaa aagaataatt ccaagcacta
     1561 acgtattcaa aggcgtaagc gagtttaaaa tagcactcac cgaactgctg atttgtgttt
     1621 gagcaataga aaacagaaaa accggaataa aagtaccaaa aactgccgtc aaagcaaggt
     1681 atttccaatg gcgtaattca atatttttga tactgttgaa tccaataatc aacagaaata
     1741 ccgcagcaaa aaccattcgc aatgaaccta attgataagg cgttaaagcc actaagcctc
     1801 ttttgataag aataaatgag ctaccccaaa taagggctaa aacgattaag taaacgaacc
     1861 ttaaattttt gttttccatc aactttttta ttgcgtcaca aaactccgtt aattttcgca
     1921 aataaaaagc gaaaaaaatg ttatttttgt attgaattac aaacaaactt tttacacttc
     1981 atttttaaaa ataaaaccta tgaaattcac aaaaaaaatc gccttatttg cctttacaat
     2041 tttatcaatt gtaagctgca aaaaggaagc aacagaaact gcaactaccg aacaaaagca
     2101 agaacaacct gctcaattag ccactgcttc ttttaccatt gaaggaatga gttgtgaagt
     2161 tggttgtgca aatcatttag aaaaaaaatt agctaaatta gatggtgttc aaaaagcagc
     2221 cgttgatttt gaagcaaaaa aagcaaccgt tgaatatgat gcaaacaaag taaccccgga
     2281 agtgctagtt caaactgttg aaggggctgc agatggtaaa acgtacaaag tatccgacat
     2341 gactaattct gctgatcatg ctcttctatt tacaaaagaa aaaaagaaaa agaaaaccgc
     2401 tgattctaaa aaagaaaacg cttctagcga agaaaaaaaa ggatgtgaac ctgaaaaaaa
     2461 atcaggatgt tgttctgcta aaaaagtgga gacattataa aaggtcataa aaaaagagtc
     2521 ctgtgggact ctttttttat gttgattact tcttccaacg catcgtttcc atcaatgtct
     2581 gcatatcgtc tttaatataa cttgatgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnngt ggctttcatt tttggatatt ctattgttac gccacaatct tcctttactt
     2761 taactttggt atcaatgtta tagtcaaaat taaacggaca aggactatca aaatccatat
     2821 aattcgccaa ttgataatct aaacgtaatt gagcggtagg tttcggcaag acctcttctt
     2881 tacaagccat aaaaaaactt atcaacatga ataatgcaaa atataaactc tttttacgca
     2941 tctaaagtca ctttaatttg ttttacccgt ttcttatcaa ctgattcaat ggtaaagaga
     3001 catttatcaa aagtgatttt ttgtcctttc ctaggaaatg tacctatgat ttccaaaata
     3061 aaaccggcta atgtttctgc ttctccttta ttattttcaa ataaatcttc atctacttca
     3121 acaattctat aaaaatcttt caagttaatc ttaccttcaa ataaaaaatt cttgtcgtca
     3181 atttgtgagt aatttatgtt ttcatcgtca aattcatcac tgatatcacc tacaatttct
     3241 tcgataatat cttctagtgt tattacccct gaagttcctc catattcatc gacaacaatc
     3301 gccaaatgat ttttcatgct ctgaaaatct tttaataaat tatccaattt tttattttca
     3361 ggtacaaaaa gtggttctct caacaaggtt gtccaatcaa attctttttt atcaatgtga
     3421 ggcaataaat cttttacgta taaaacgcct tcaatctgat caatattgtc tctatacacc
     3481 ggaattcttg aatacccttt gtctaaaatt ttaataataa cctcagtaaa tgattcctct
     3541 atttccaagg caaacaagtc tattctcgga ctcatcactt ggctagtatc cgtgtttccg
     3601 aaagacacaa ttccttccaa tattttttgt tcttcttgtg atgtatcttc agacgatgtc
     3661 aattccaacg cctgggacaa ttggttaacc gaaatattag atttttgttt acctaattta
     3721 ttttccaaat agattgtaat ggctcgcatt ggcatattaa ttggtgtcaa caacatattc
     3781 aaaatagcaa ttggagatgc tatcttttta gcgaatttta cattgtttcg gttagcataa
     3841 accttgggca aaacctcccc aaaaaataaa attaaaaagg taacaacacc tacttcgatt
     3901 gcaaaacgaa gccagtcaaa cttaatttga ctaaataaat cttcttttat aaaagagaaa
     3961 atgataacaa taccaatatt aataaaatta tttgcaatta ataaggtagc caataatttt
     4021 tttggtttgc taagtaaggt agaaataata tttcctttgg attggttttg agcagttgcc
     4081 tcgtcgatat ctttattgga taatgaaaaa agagcaactt cagatccaga gattaaggca
     4141 gaaaataaaa gtaaaacgag aatgcccaaa attcctaaga gcaaactcgt atctatattt
     4201 tctaacaaac tcgacggttc agagtccaaa tttaattata ttgattagac attaataggg
     4261 taaatcatct tgattaccgc tgcctctttg ggcttcaaaa ttagtgtttt tatttggttc
     4321 tgtcattgaa ggaacctgtt gtttatttaa ctctgtttct ttctttgtag ttaaaaaagt
     4381 aaattcagtt acttgaattt ctgtagtgta ttttgtttga ccatcttccg cttgccattg
     4441 tctggatttg attcgtcctt caacataaat tttatctcct tttgaaaggt atttttcgca
     4501 aatttcagcg gctttatttc gaacaactaa gttatgccat tccgtagatg ttattttttc
     4561 attggtagtt ttattaatat aaacttcatt tgttgccaaa ggaaacctac caatacaatt
     4621 accaccatcg aaataatgca tttttacatc gtcacctaaa tgacctatta acataacctt
     4681 atttaaagtt ccattcataa tattttgttg tttttggaca gtatcaaagt tacgaattta
     4741 ataacagttt aaaaatactt ttcaataaaa ttaaaaatca caatagggaa aggtctcttt
     4801 ttgatagatt gttttgactt tgcattttcc aattttcctt tcaattgaat attccaaaat
     4861 ctgattttca accgttgatg ggataaaata tgcaatacac ttttttcttc ataggcatta
     4921 atagacatta tttcatttga aaaaaatttc attttattga tttcatctgt aatttctttc
     4981 tctgtaattt caataaaaga ttcaattaag ggaaattcgt atagattatg ccaaattcct
     5041 ttttcggtcc gctgttgaat aattgaattt ccgagttcat cttcaaatac caaataatta
     5101 aaaaaacgtt cggtcacttt tgtctttttt aatttaactg ggagctgatt tattttattt
     5161 ttttgcaatg cgacacaact atcattcaaa acacaaactg aacaatttgg attttttggc
     5221 atacattgca acgctccaaa ttccataata gcttgattaa actgcaaaga attcgttttg
     5281 tctaatactt cataagccaa ggctgtaaac tcttttttgg ctattgttga ggaaatatca
     5341 gtctccacat caaaataacg agctaacacg cgaaaaacat tcccatctac aacaggaacc
     5401 ggctcattag aactaaaaga ggcaattgct gcagctgtat atgttccaat tccttttaat
     5461 tttaataatt cctgataaga agtcgggaaa ttcccaccca aatcaaaagc aaccagttga
     5521 gctgttttat gaagatttcg agcacgagaa taataaccga gtccttgcca aagttttaaa
     5581 acttgctctt ctgatgcatt tgctaaatca aatactgttg gaaatgtctc cacaaaggct
     5641 atataataag gcaaaccttg ggcaactcgc gtttgttgta agatgatttc tgaaagccat
     5701 attttgtaag gatttgtagt gtttcgccaa ggcaaatctc gtttattttc taaataccaa
     5761 gatataagcc gattagaaaa agtcatattt ttaatttggt taacaaaagt ataagtttat
     5821 gtgattaaat tttaatcaat tatgcttgaa taattgtttt tttaatttgt atatttgcac
     5881 tctcaaaaat ttacacataa ataattaata cgaaagaaaa tgacgaaagc agatatcgta
     5941 gcgaagattt cagaaaaatt aggacttgaa aaaggtgatg ttcaagcaac tgtagaatct
     6001 tttatggaag aagttaaaaa ctcattagaa actggcgaca atgtttattt aagaggtttc
     6061 ggaagcttta ttatcaaaac aagagctgaa aaaacaggaa gaaacatttc aaaaaacaca
     6121 acaattaaaa ttcctgctca caacattccg gcattcaaac cagcaaaggt ttttgttgaa
     6181 ggtgtgaaaa ccaacactga agtagcagta taactcattt attaatacca aaaagatcac
     6241 actatgccaa gtggaaaaaa gagaaagaga cataaggtag cgacgcacaa acgtaagaaa
     6301 agagcgagag ctaaccgtca caaaaagaaa aagtagtgta aactactttt ttcttttaca
     6361 aaaagttctt tgaaatgaaa cagtaaaaaa caataactgt ttcaccgggt acaaaactac
     6421 ctgtttaaaa tattgtttaa tccatcctta caattaggcc ttaagcttta tgctataagc
     6481 tttaagcaat ttaaattgcc tatcgcctat tgctattgcc tattgctaaa aagttcggat
     6541 aaaaatttac aacgtgaata aagaattgat catcaggtca ggttctgaag ctgtagattt
     6601 tgccttatta aaagatggaa aactagtaga attacacaaa gaagaagagc aaaaaagcgg
     6661 ctttcaggtg ggtgatattt ttatcgccaa aatcagaaaa ccggttcctg ggcttaacgc
     6721 ggcttttgtg catttaggtt tcgaaaaaga tgccttttta cactaccacg atttgggtcc
     6781 aaacttatct tcgatgttga aattcaccaa acttgtaagc acaggtaaac aaaaagattt
     6841 ctcccttaaa aactttcagt ttgaaaaaga aattgacaaa gatggcacca tcaccgatgt
     6901 gttgagtgca aatcagtcgg ttttagtaca aatcgttaaa gaacctatct ctaccaaagg
     6961 tccaagaatt agctctgagc tttctctggc cggaagatat atagttttag tgccgttttc
     7021 tgaccgcgtt tctgtttcac aaaaaataga atcgaaagaa gaaaaagacc gtttaaagaa
     7081 acttgtacaa tctatcaaac cgaaaggatt tggtgttatt gttcgcacag tagcagaagg
     7141 caaaaaaata gccgaattag accaagacat gcagaattta ctcagtaaat ggactgccat
     7201 gtgtaaaaaa ttaccaacgg ctcatcatcc gtctaaagta ttaagtgaaa tgaacagagc
     7261 ttcatcaata ctcagagacg tatttaatga ttccttcacc ggaatttata ttgatgatga
     7321 agagttgttt gagcaaacca aagaatcaat tgaagaaatt gatccttcaa aagtgaacat
     7381 tgtcaaactt taccaatcaa aggaattgcc catttttgaa aaataccata ttgaaagaca
     7441 aatcaaaacg tcttttggca gaacggtatc aatgagcaaa ggagcttatt taattattga
     7501 acatactgaa gcacttcacg tcatcgatgt gaatagtgga aaccgttcca ataaagctca
     7561 aaatcaagaa gacacagccc tggaagtaaa catgattgca gcagcagaag ttgccagaca
     7621 attgcgttta cgggatatgg gtggaattat tgtagtcgat tttatcgata tgcaaaatcc
     7681 ggaaaaccga aaagtccttt ttgatttctt gcgagaagaa atgagcgatg acaaagcaaa
     7741 acacaaaatc ttaccgccga gtaagtttgg attaatccaa atcacacgac aacgcgtaag
     7801 accggaagtt aacattaaaa caagagaaga agatcctaac aatggaacag gagaaataga
     7861 tgctccaatt caagttattg ataagatcaa tctcgatgtt gaacgactaa aaaatcataa
     7921 aaagctggtg cttaatgccc atccttttgt ggccgcatac ctaaccaaag gttttccatc
     7981 attacgttca aaatggtttt ttgaacataa aaaatgggtg aaaattatac ctcgtgatgc
     8041 ttacacgttt ttacaatacc aattttatga taaagaaggt aatgtaataa aagaataaaa
     8101 aacgaacgtc tgccttttgg tgggcgtttt tttttggctt tttgccatga attaaaagaa
     8161 tttacactaa gttattaagt ttgctacttt ctttctacta attttgaagt atacttattc
     8221 agatgaaatt ttatttattt agttgctttt tttgttggat tattttccaa aatacaagtt
     8281 cagctcaatt gggttgcacg gatgttttgg ctagaaatta taatcaaaat gcagttgaaa
     8341 acgatggtaa ttgctgttat aaaagactaa aaattaagaa atttacttca gttgttttga
     8401 acgatatgct caatgaaacc tctagtttgg tggagtggga aaataacctt tatacacata
     8461 atgacgacac cgatttacat ctttatcaat tggataaaaa tggttctatt acaaaaagaa
     8521 ttaaattaaa cggtattaaa aacaacgact gggaagaact tacgcaagac gaaacccact
     8581 tttatttggg tgatttcggc aataatgcta gcggaaacag aagcgattta accattttta
     8641 aaatagccaa aaactcctta tttgataatc caaaaataga aactatttct ttttcatatc
     8701 ccgaacaaac cgatttttcc aaacaaaaaa gcaacactac taactttgac tgcgaagctt
     8761 ttattgccac tgaaaatgaa ctgattctat ttaccaaaca atggaaaaac aatcaatccg
     8821 ctgtttatcg tttacccaaa acagccggga aatattcagc agaatacatt acaacactaa
     8881 aagtaaacgg attgattacc ggagcaactt ttgtagaagt gaaaaacctc atcactcttt
     8941 gtggacatac caaaaagcta aaaccattta tgtatttgat ttacgatttg aaaaatgtaa
     9001 actttgaaaa tattaaccaa agaaaaataa aattgaaatt gccttttcat caaatagaag
     9061 ccattagttc aatcaacggt attgattttt acatcactaa tgaacggttt tcaagaaaaa
     9121 ttattggaac ggttttgcaa caactgcatc atttttcgtt agaatttatg tcaagataag
     9181 ccaaagactc actcctgctc cacttccact tttcgcttaa tttcatttcc tttttcatca
     9241 actacggtaa cataatgtat tccggatgca actatcagtg gcatttcatg aaaattttgt
     9301 gtgattccca aataggtatt atccacatac caatacaatt ttatattagg ttgcgaatga
     9361 gccacttt
//
LOCUS       DAOTCW010000087         9358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_4986_length_9358_cov_38.246265, whole genome shotgun sequence.
ACCESSION   DAOTCW010000087 DAOTCW010000000
VERSION     DAOTCW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9358
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4986_length_9358_cov_38.246265"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..693)
                     /gene="ssrA"
                     /locus_tag="P5335_07900"
     tmRNA           complement(295..693)
                     /gene="ssrA"
                     /locus_tag="P5335_07900"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            897..2099
                     /locus_tag="P5335_07905"
     CDS             897..2099
                     /locus_tag="P5335_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRZ74838.1"
                     /translation="MTFGIIKERKNPPDRRVVFTPEELVRLQNQFPQAKIKIEASDIR
                     VFPDTAYADLGLEVTTNMTDCDVLIGVKEVPVEALLPNKKYFFFSHTIKKQPHNRKLL
                     QAILDKNIELYDHETIVDAQNKRLIGFGRYAGIVGTYNAFRGFGIKYELFSIPKAETL
                     KGKDELIAKLKRQILPPIKIVLTGKGKVGMGAKEILDAIKIKEVSVDNFLNKTYSEPV
                     YVQIDVLDYNKRKDGQQLGNNDFYKNPQDYTSDFERFTKVSDVFIAGHFYGNNAPHIL
                     TNDMLKAADCKIKIVADISCDVDGPIACTIKASTIAAPFFGYLPLESKEVSYTHPAAI
                     LVMSVDNLPCELPKDASEGFGEMFMEHVIPAFFNGDKEGILQRAKITESGKLTPRFSY
                     LQDYVEGK"
     gene            2183..>4773
                     /locus_tag="P5335_07910"
     CDS             2183..>4773
                     /locus_tag="P5335_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRZ74839.1"
                     /translation="MLKKYCLYLFLLWSSLNCFSQELLPFVENFTKSNYNGDNQIWSV
                     TQGNDNAMYFANNHYFLRYNGVKWDKYILPNKTIIRSVLSDGNRIYCGSYTEFGYWIR
                     TNGEMIYHSLSKGKGLFSGYSNNEEIWKIFKFNNTIYFQSFNDLFVYNGKTIEKIRIP
                     FQISYCFVVDNKIYAASVRNGIYEFENKKFTKIEKWKIVENNIVHSIEKNQNTLFVFT
                     QKNGVFVERNGELFSWQNSLNEELKNEIIIVAKFIDSNRLIIGTAFKGLYLIDTKTGT
                     FQNISRNNSLKNNSVLSIGLDKENDLWLGLDNGISHIEINSPYSIFSDNTGILGSVYS
                     VSFLNDGYLLGSNHGVFKYNNKQLELLPNSQGQVWDIQKIDNQYVIGHNDGTFLYDNN
                     QLKRVNAVSGGWKLLNSKFDSKLYQANYSGIVMYNNPTDLTQYEIIRNLTKPIKNIAQ
                     NKPYELWAVDNYRSLYRIKFKKDYQLDKIENITQKNNIKNDFEVKLFHFKNEMLFFID
                     NTWYHYNSLAEKLEKNSLFNQNFSTVTEFVPVDDENFILLKDKLLYIIHQVDGKFIWE
                     LIPEKYYEGKIINQDTKVFRKGNQLIMNLDDGFFTFQLKNSKLKKQNVVIEAFSQGTK
                     IKENETIRHNQSLEINVISEYFGYNRSGLFYKLNGLDTFQPIQNGNLILNNLSSGNQK
                     LEVFYNNGENYKLISNFKFAVAKPWYFSIWMILIYFSLIAGTFFLYYKWNKVRYLEQL
                     KLKEEELKHQKEIIQLELEAENKLKLQEYEKHILEIQVQTKATEVAGKSLSIAKQSEM
                     IETIKKILDSESDLAQLKSKINKSIKVNAINKKEWESFEKNILQSNDEFVHRLTNLYP
                     SLTSKDI"
     assembly_gap    4774..4873
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4874..5085
                     /locus_tag="P5335_07915"
     CDS             <4874..5085
                     /locus_tag="P5335_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRZ74840.1"
                     /translation="FVHRLTNLYPSLTSKDIKLCIYLKMNLSSKEIAPLMNISYRGVE
                     LHRYRLRKKLNLHSDDNLSNFMINL"
     gene            5340..8303
                     /locus_tag="P5335_07920"
     CDS             5340..8303
                     /locus_tag="P5335_07920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRZ74841.1"
                     /translation="MKRYLLIIVVLFIQTFVQAQEISGKVTDASGMPLPDVIVLVVKT
                     QENTVTDFDGNFKIKVEVGDEIQFSMLSFQKIVVKASPNMVVVMKDDDQKQLGEVVVV
                     GYGTKKLGSITGSVSQIKSAEILKTPAQSAIQSVQGKLAGVNIVTNDEPGSTPSIQIR
                     GLGTLLGGRNPLYIIDGIESTSLNGLSPNEIASMDILKDASSLAIYGQKGSNGVIIVT
                     TKKGKEGKFKVNFDSYYGQKFIQREVKMADSYRFVYYNNSALGSSSYFNFEQPYNTNW
                     LDEITDTGTVMSNFLSISGGSQNANYYLGYTNYKEEGILNGTDYERNNINSRMEVKLF
                     DDRLKISNAINFSIVKNTPKPLSAFTNAYKQSPIMPVQYPNGRWGMPFINEDGVNDYN
                     GTRYNNVANPVAQLYYTNEQNKNFVVFGSINAELKITDDLKFTTNFGTTYDIGEGYTF
                     TPSRDIWLSQNPTQEIEDYPLSNPNNVLNQRKYDSFIWNWDNYFTYTKEFGKNEITGV
                     LGMSRSTRNNSQYISGTRFNVPEQSNYWYLDFSSYNINTSPGAVVSNSHTTPVVSIAY
                     FARADYEYDGKYLLTASLRREGISSFQDDEQWGLFPAISAGWVMSNEPFLKDVKFLNF
                     LKLRGGYGEVANGNSGNSLNTLVFGSGYNYAFGADQEIFPGANIPYQVDPSLSWETMK
                     EIDFGFDFKVLDNKLSGTFDIYNRNAVDVILPVSLPPVLSPGAVYLNTGTVSNNGIEL
                     SLRWEDKINEDWRYSIGTNYSHNKNELKDVSNAYFSNLIGGSLGNGQWTKQVLVGEPL
                     GSFYVYDVTGYNSDGGFTYSDERVVAGSYLPTYTYGVNLALYYKSFDFSTDLFGVGGN
                     KLYNGKKAQRFGGENIEYDILDSFWTPSNPNADNPYPFNEVPIASTYYIEDGAFLRVN
                     NITLGYVVPNFYEKIDKIRFYVTAVNPFIFTKFSGYSPEISGNNNADPLGNAGIELDA
                     YPTNKTFFFGLNVSF"
     gene            8314..>9358
                     /locus_tag="P5335_07925"
     CDS             8314..>9358
                     /locus_tag="P5335_07925"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRZ74842.1"
                     /translation="MKNKILYNLLLILSISFVSLSCEEDLNVEPNNAISEFDFLNNPD
                     NAVQLVNGVYNKQLDYNMYCFSWIGMTSITSDDADKGSTPNDTGTDKHKMDNLTFNAT
                     DISFIDVWEGRYDGIYRANNALFYLEQLTIDPTLKSRLIGEVKFLRALYYFDLVRCFG
                     GVPLVTTKIDINNTEEINNIVFVRKSKEETYAQIETDLTDAIERLPIKGQYSPNDLGR
                     ASKGAAQALLAKAYLYQEKWQLSYDLAGEVITSGQYGLLANYAEVWREVGENKVESIY
                     EVQATLTKGLVGYTDVQGARGTPDLGWGFNTPSLSLVNSYQAGDVRKNATVLFVQSTP
                     FTLWDGFEGSPTWN"
BASE COUNT         3242 a         1511 c         1564 g         2941 t
ORIGIN      
        1 gcttttgtac tttttacaaa aaaaagtata gataatttat gtttcatttt aggtaacctt
       61 ttagattaaa caatttacat ttttttgtct ttactgtcaa gatgttcatc ttttgaacct
      121 cttgccatta aagggctggc gtgaaattcg gtgtcccaaa atttgatttt tatggggaca
      181 ccgattatag cacctaaaga atgatttaat ttgaataaat tgcgaagtgc actaaaacat
      241 aaaaccctgc aaatcataag atttgcaggg ttttagtcaa taatgtaagt aatactgtgg
      301 agtcggagag aatcgaactc tcgtccaaac aggcaaccaa agagctttct acgcgtttat
      361 cctctgattg agttttcgac aaaaagcaag gccaaaggca gccacttctt gcttagctta
      421 ataagtgtca gaagggattc aaagcattac ccttcctagg tttattttta cggttcacct
      481 gtttcaaacg ccacaaacca aggctttcga ggtgaatcca gctttcctac ctagtaggaa
      541 cgaggcaaat cttactataa ttcagattaa gcagctaaag cgtaattatt ttcgccgttt
      601 ataagttgtg aaccttgata tttacgagcc aaatgtccaa tgctcgacgt gcttaccgtc
      661 tagttccacc cgctgtcaaa accggtcgac cccatttttt ctgtttttta ttaacaattg
      721 tctgttatcc actgcttgtt tctcgcaacc cgcaactcgt aactcgcaac acaattcatt
      781 atcaaaaacc agaccacaaa gatacatgaa aatttggcag aaataaaact tgcctgactt
      841 attctataat gttacatttg aaacaatttt taaatattgc actaaaaata tcacaaatga
      901 cattcggaat catcaaagaa cgcaaaaatc cacccgacag aagagttgtt ttcaccccgg
      961 aagaattagt ccgattacaa aatcaatttc cgcaagcgaa gataaaaata gaagcctcag
     1021 acatcagagt tttccccgat accgcctacg ctgacctagg cctcgaagta acaacaaaca
     1081 tgaccgattg tgatgtgtta attggtgtaa aagaagtacc cgtagaagct ttacttccca
     1141 ataaaaagta ttttttcttc tcccatacca tcaaaaaaca gccgcacaat agaaaattat
     1201 tacaggctat cctcgataaa aacattgaac tatacgatca cgaaaccatc gtcgatgctc
     1261 agaacaaacg cctaatcggt tttggcagat acgccggaat cgtcggcacc tataatgcct
     1321 tcagaggctt cggaatcaaa tatgaattgt tctccatccc aaaagccgaa acattaaaag
     1381 gaaaagatga gttaatcgca aagctaaaaa gacaaatact ccctccaatc aaaatagtac
     1441 tcaccggaaa agggaaagta ggcatgggag caaaagaaat tctcgacgcc atcaagataa
     1501 aagaagtatc agtcgataac ttcttaaaca aaacctattc agaacccgtc tatgttcaaa
     1561 tcgacgtatt agattacaac aaacgaaaag atggacaaca actgggaaac aacgattttt
     1621 ataaaaaccc ccaagattac acctctgatt tcgaacggtt cacaaaagtg tcagatgttt
     1681 tcatcgccgg tcatttttat ggaaacaatg ctccccacat tcttaccaat gatatgctca
     1741 aagccgctga ttgtaaaatc aaaatcgtcg ctgatatttc ctgcgatgtc gacggtccaa
     1801 tagcttgcac catcaaagct tccaccatcg ccgccccatt ttttggttat ttaccacttg
     1861 aaagcaaaga agtttcctat acccatccgg ccgcaatttt agtcatgagt gtcgataatt
     1921 taccctgtga attaccaaaa gatgccagtg aaggtttcgg cgaaatgttt atggaacatg
     1981 taatccccgc tttttttaac ggtgataaag aaggcatttt acaacgagca aaaatcaccg
     2041 aatccggaaa actaacgccc cgtttcagtt atttacaaga ctatgtagaa ggtaaataaa
     2101 atacaaaaat cacccgttta cttcacctaa ttaaatggtt tagtccaatt agttttttta
     2161 catttgagga atattaaacc caatgttgaa aaaatattgc ctttatcttt tccttttatg
     2221 gagttccttg aattgttttt cacaggaatt acttcctttt gtcgaaaatt tcaccaaatc
     2281 aaactataac ggtgacaatc aaatttggag cgtaacccaa ggaaatgata atgcgatgta
     2341 ttttgccaat aatcattatt ttttaaggta caatggcgtc aaatgggata aatatatcct
     2401 acccaacaaa acaattattc ggtctgtttt atctgatggc aacagaattt actgtggctc
     2461 ctacactgaa tttggttatt ggattagaac taacggtgaa atgatttacc actccctaag
     2521 taaaggaaag ggattgtttt cgggctattc gaacaatgaa gaaatctgga aaatatttaa
     2581 attcaacaac accatttact ttcagtcatt caatgacctt ttcgtttata acggcaagac
     2641 cattgaaaaa atcagaatac cattccaaat ttcttattgt tttgtcgtag ataataaaat
     2701 ctatgccgca tccgtcagaa atggaatcta tgaatttgaa aacaaaaaat tcaccaaaat
     2761 tgaaaagtgg aagattgtcg aaaataatat cgttcattcc attgaaaaaa accaaaacac
     2821 cctttttgtt tttactcaaa aaaatggcgt tttcgtagaa agaaacgggg aattattttc
     2881 atggcaaaac agtttaaatg aagagttgaa aaatgaaatt atcattgtgg caaagttcat
     2941 tgattccaat cgattaatta taggaactgc ttttaaagga ttatatttaa ttgatactaa
     3001 aactggcact tttcaaaaca tcagccggaa taattcactc aaaaacaact ctgttttaag
     3061 tatcggatta gataaagaaa atgacctttg gctaggtctc gacaacggaa tatcacacat
     3121 cgaaatcaat tcaccctatt ccatattttc agacaataca ggaattttag gctctgttta
     3181 ttccgtttca ttcttgaacg acggctattt gttaggctca aatcacggag tttttaaata
     3241 caataacaaa cagttagaac tactgcccaa ttctcaaggt caagtctggg atattcaaaa
     3301 aatagataat caatatgtaa ttggacataa cgacggtact tttttatatg ataacaatca
     3361 actcaaacga gtaaatgcgg tgagcggtgg ctggaaactt ttaaacagca agtttgattc
     3421 taaactatat caggcaaatt attccgggat tgtaatgtac aacaatccaa ctgatttaac
     3481 ccaatatgaa atcatccgga atttgaccaa acccattaaa aatattgctc aaaacaaacc
     3541 atatgaactt tgggcggtcg ataattacag aagtttatat cgaattaagt tcaaaaagga
     3601 ctatcaacta gataaaattg aaaacattac tcaaaaaaac aatatcaaaa atgattttga
     3661 agtaaagctt tttcatttta aaaatgagat gctgtttttc attgataata cttggtatca
     3721 ttacaattca ctggcagaaa aattagaaaa gaatagttta tttaatcaaa atttttcaac
     3781 ggttacagag ttcgttcctg tagatgatga aaactttatt ttgctcaaag acaaactgtt
     3841 atatattatc catcaagtag acggaaagtt tatttgggaa ttaattcctg aaaaatacta
     3901 tgaaggaaaa atcattaatc aagacacaaa ggttttcaga aaaggaaatc aattaatcat
     3961 gaatttggat gatgggttct ttacctttca attgaaaaat tctaaattga aaaagcaaaa
     4021 tgtggtaatt gaagctttca gtcaaggtac taaaatcaaa gagaacgaaa caattagaca
     4081 taatcaatca ctcgaaatca atgtcatttc agaatatttc ggatacaacc gttccggatt
     4141 attttacaaa ctaaatggtt tagatacttt tcaacccatt caaaacggga atttgatatt
     4201 gaacaacctt tccagcggga accaaaaatt agaagttttt tacaataatg gtgaaaatta
     4261 taagctaatt tcaaacttta agtttgcggt tgctaaacca tggtattttt caatttggat
     4321 gatactgatc tatttttcat taatagcagg aacttttttc ctttattata aatggaataa
     4381 agtgcggtat ttggaacaat taaaattaaa agaagaagaa ctcaaacatc aaaaagaaat
     4441 tatacaactt gaattagagg ctgaaaataa attgaaatta caggaatatg aaaaacacat
     4501 tctggaaatt caggtacaaa cgaaagctac agaagtagcc ggtaaatcat tatctattgc
     4561 aaaacaatca gaaatgatag aaaccataaa aaagatctta gattcagaat ctgatttagc
     4621 acaactgaaa agtaaaatca ataaatcaat caaagttaac gccatcaata aaaaagaatg
     4681 ggagagtttt gagaaaaata ttttacaaag taatgatgaa tttgtacatc gcttgacaaa
     4741 tttatatcct tcacttactt caaaggatat taannnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnaatttgt acatcgcttg acaaatttat atccttcact tacttcaaag
     4921 gatattaagc tctgtattta cttaaaaatg aatttatctt ctaaagagat tgccccttta
     4981 atgaatattt cctatcgggg tgtagaactt catcggtacc gattaagaaa aaaattgaac
     5041 ttacactcag acgacaactt gtctaacttt atgattaatt tataaaaaat cttttaaatt
     5101 tattatttaa tcatttttta ctttaaaaaa ttaaagaatt attacacatc attactacat
     5161 caaatcgcta tttaaacgat gtgtttttaa tgattatttt taattatata ttattaaaaa
     5221 tcaagtaaat aaaaaatatt tttattgaaa tgatgtagta ctgtagtaaa ttaattaata
     5281 caactatatc gttttagtta gtatatttac acaactaaca caaacaaact acatttttta
     5341 tgaaaaggta tttactaata atagtagttc tattcatcca aacatttgtt caagctcagg
     5401 aaatttcagg taaagtaact gatgctagcg gtatgccatt gcctgatgta attgttctag
     5461 ttgtaaaaac acaagaaaat acagtaactg attttgatgg taatttcaaa ataaaagtag
     5521 aagttggcga tgaaattcag ttttctatgt tgagttttca aaaaatagtt gtgaaagctt
     5581 ctccaaatat ggttgtcgta atgaaagacg atgatcaaaa gcaattagga gaggttgttg
     5641 ttgtaggata tggaacaaaa aaattaggat cgattaccgg ttcagtttct caaataaaat
     5701 cggctgaaat acttaaaact cctgcacaat ctgctatcca atctgttcaa ggaaaattag
     5761 ccggggtaaa tattgttaca aatgatgaac cgggttcaac accgtctatc caaattagag
     5821 gtcttggaac tttgttagga ggtcgtaatc ctctatatat tattgatggt attgaatcaa
     5881 cgagcctaaa cggattaagc ccaaacgaaa ttgcttccat ggatattttg aaagatgctt
     5941 cttccttagc tatttatggt caaaaaggtt ctaatggtgt aatcattgtc accactaaga
     6001 aagggaaaga aggcaagttt aaagtcaatt tcgattctta ttacggacag aaatttattc
     6061 aacgggaagt aaaaatggca gattcttacc gatttgtcta ttataataat tcagcattag
     6121 gatcaagttc ttattttaac tttgaacagc cttacaatac taattggctt gatgaaatta
     6181 ccgatacggg aaccgtgatg agtaatttcc tttcgatttc cggtggaagt caaaacgcta
     6241 attattattt agggtataca aattataagg aagaaggtat tttgaatgga acggattacg
     6301 agcgtaataa tattaattct cgtatggaag ttaaattatt tgatgatcga ttaaagatat
     6361 caaatgctat aaatttttca atcgtaaaaa acacgcctaa acctttgagt gcatttacaa
     6421 atgcctacaa acaatctcca atcatgccgg ttcaatatcc taatggaaga tggggaatgc
     6481 cgtttataaa tgaagatggt gtgaacgatt ataatggaac acgttataat aatgttgcga
     6541 atccggtagc tcaactttat tataccaatg aacaaaataa aaactttgtt gtttttggct
     6601 ctattaatgc tgaattaaag attacggatg atttaaaatt caccacaaat ttcggtacga
     6661 cctacgatat tggtgaggga tatacattta ccccaagtcg cgatatttgg ctttctcaaa
     6721 atccaactca ggaaattgag gattaccctt tatctaatcc taataatgtt ttaaaccaaa
     6781 gaaagtatga ttcttttatt tggaattggg ataattattt tacttacacc aaagaattcg
     6841 gtaaaaatga aatcactggg gttttaggga tgtcacgatc aacccgaaat aatagtcagt
     6901 atatttcagg aactcgtttt aacgtaccgg aacaatctaa ttattggtat ttagattttt
     6961 catcttataa cataaatact tctccgggtg ctgttgtttc taattcacat accactccgg
     7021 ttgtttccat cgcctatttt gcaagagctg attatgaata tgatggaaaa tatttactga
     7081 ccgcttcatt aagaagagaa ggaataagtt cttttcagga tgatgaacaa tggggtttat
     7141 ttccagcaat atctgccggt tgggttatga gtaatgaacc ttttttgaaa gatgttaaat
     7201 tcttaaactt tttaaaactt cgtggaggtt atggtgaagt tgccaacggg aatagtggaa
     7261 attcactcaa tacgttagtg tttggttctg gttataatta tgcttttggt gcagatcaag
     7321 aaatttttcc gggagcaaat attccgtacc aagtggatcc ttctttgtca tgggaaacga
     7381 tgaaagaaat tgattttgga tttgatttta aagtacttga caataagctg tccggaactt
     7441 tcgatattta taatagaaat gctgttgatg taattttacc agtttcactt ccgccggttt
     7501 tgtctccggg agctgtttat ctaaacactg gaacggtttc taataatgga attgagctat
     7561 ctcttcgttg ggaagataaa atcaatgaag attggagata ttccatcgga accaattatt
     7621 cacacaataa aaatgaactg aaagatgtga gcaatgctta tttttctaat ctaattggtg
     7681 gaagtttagg taatgggcaa tggacaaaac aagtgttagt tggtgaacct ttaggcagtt
     7741 tttatgttta tgatgtcaca ggttataact cagatggtgg atttacttat agtgatgaaa
     7801 gagtagtggc aggttcttat ctaccaacat atacctacgg agtgaattta gcgttatatt
     7861 ataaaagctt cgatttttca actgatttat ttggcgttgg aggaaacaaa ttatacaacg
     7921 gtaaaaaagc ccaacgtttt ggtggtgaaa atatagaata tgatatttta gatagttttt
     7981 ggactccttc taatcccaat gcagacaatc catatccttt taatgaagtt ccaattgctt
     8041 caacatacta cattgaagac ggagctttct taagagtaaa taatatcact ttaggttatg
     8101 ttgttcctaa tttttatgaa aaaatagaca agataagatt ttatgtaacc gctgtaaacc
     8161 cttttatttt tactaagttt tcaggttatt ctcctgaaat atcaggaaat aataatgctg
     8221 atcctttagg aaatgcaggt attgaattgg atgcttatcc tacaaacaaa accttctttt
     8281 ttggtttaaa tgtttccttt taaaactact atcatgaaaa ataagatact atataattta
     8341 ctattaatcc tttcaatctc ttttgtttca ctttcttgtg aagaggattt gaatgtagaa
     8401 cctaataacg caatttctga atttgatttt ttgaataatc cggataatgc agtgcagttg
     8461 gtaaatggtg tttataacaa acaattagac tacaatatgt attgcttctc ttggatagga
     8521 atgacaagta ttacttcgga tgatgctgat aaagggtcaa ctccaaacga taccggtacc
     8581 gataagcata aaatggataa cctaactttt aatgcaacgg atatttcatt tatagatgtt
     8641 tgggaaggtc gttacgatgg tatttataga gctaataatg ctttgtttta cttagaacaa
     8701 ttaaccattg accctacttt aaaaagtagg ttgattggtg aagttaaatt tttaagagcg
     8761 ttatattact ttgatttagt aagatgtttt ggtggtgttc cattggtaac tacaaaaatt
     8821 gacattaaca atacggaaga aatcaacaac atcgtttttg ttcgaaaatc aaaagaagaa
     8881 acgtatgctc aaatagaaac tgatttaacc gatgctattg aacgattacc tataaagggt
     8941 caatattctc ctaatgattt aggaagagcc tctaaaggtg ctgctcaggc tttattagca
     9001 aaagcctatt tgtatcaaga aaaatggcaa ttgtcttatg acttggctgg tgaagtcatc
     9061 acttcaggtc aatatgggtt attagctaat tatgctgaag tttggcgtga agttggcgaa
     9121 aacaaagttg aatctattta tgaagtgcaa gccactttaa caaaaggact tgttgggtat
     9181 accgatgttc aaggagccag aggaactcct gatttaggtt ggggtttcaa tacaccttca
     9241 ttatcgcttg ttaattctta tcaggcaggt gatgtaagaa aaaatgcaac agttttattt
     9301 gtacaatcaa ccccttttac attatgggat ggttttgaag gttctcctac ttggaata
//
LOCUS       DAOTCW010000088         9336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_5004_length_9336_cov_46.080918, whole genome shotgun sequence.
ACCESSION   DAOTCW010000088 DAOTCW010000000
VERSION     DAOTCW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9336
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5004_length_9336_cov_46.080918"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="P5335_07930"
     CDS             complement(<1..269)
                     /locus_tag="P5335_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRZ74843.1"
                     /translation="MAKRLSNESTISDVLKAFIENNKLQSGMDKIDVEKAWKDLMGNG
                     VNTYTKEVILKGSTLYVTLTSSVLREELSHGKQKIIIMLNDELRK"
     gene            complement(269..688)
                     /locus_tag="P5335_07935"
     CDS             complement(269..688)
                     /locus_tag="P5335_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74844.1"
                     /translation="MKHFFYSILILVFFISCQEKVTLESISKINGYWEIQKVELPDGQ
                     KKEYKINETVDYFEWKESVGFRKKVTPQFDGTFLINDELEEIQIKDSSGIFQIHYKTP
                     YTQWKEEIITLTDSILVLKNKQNLEYHYKRFTPFSIK"
     gene            800..1873
                     /locus_tag="P5335_07940"
     CDS             800..1873
                     /locus_tag="P5335_07940"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRZ74845.1"
                     /translation="MKKYFSFLLISILFLNCSSDSDSPEENPSDSMYFPPISGTTWET
                     KSMADLGWNESEVQPLLNYLEEKNTKGFIILVNGRIVMENYFNDHNANLPWYWASAGK
                     TLTTATIGIAQQEGLLSINDKVSDYLGIGWTSAPTNKENLITNKHLLSMSSGLDDSFG
                     DGNDPIDLQYKADAGTRWAYHNVFEKLQDVIANASNQTFDSYFTSKLKSKIGMTGAWI
                     ESDEFNVYWSTTRSTARFGLLSLNKGKWNSEVIVPEAYMLEAMSTSQTINNSYGYLWW
                     LNGKANYRLPQTQLQFPGSLIPNGPADMVCGLGKNDQKIYVIPSKKMVVIRLGDVANP
                     DNPTFALSGFDNELWQKINAVIN"
     gene            complement(1870..2169)
                     /locus_tag="P5335_07945"
     CDS             complement(1870..2169)
                     /locus_tag="P5335_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74846.1"
                     /translation="MEQQEPKVFNKYLVSLTKIVSVYCFFFVLLKSVAIVRGAWMIPN
                     VVMAIPFMIFGIWTAYTVIYKKYSWAIAILGVVLIIITRYYEYDWVYFLQNKYGN"
     gene            complement(2169..3122)
                     /gene="ftsY"
                     /locus_tag="P5335_07950"
     CDS             complement(2169..3122)
                     /gene="ftsY"
                     /locus_tag="P5335_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRZ74847.1"
                     /translation="MSFFKRIFSSEKKETLDKGLEQTKTSFFAKLTKAVAGKSSVDDD
                     VLDNLEEVLVSSDVGVKTTLKIIDRIEARVARDKYMGTDELNKILREEIAGLLSETNS
                     GEATEFDIPSNKKPYVIMVVGVNGVGKTTTIGKLAYQFKKAGYKVVLGAADTFRAAAI
                     DQLQIWADRVGVPIVKQQMGSDPASVAFDTLQSAVTQGADVVIIDTAGRLHNKVNLMN
                     ELTKVKRVMQKVVADAPHDVLLVLDGSTGQNAFEQAREFTAATEVSCLAVTKLDGTAK
                     GGVVIGISDQFQIPVKYIGVGEGIEDLQVFNKYEFVDSFFK"
     gene            complement(3221..3373)
                     /locus_tag="P5335_07955"
     CDS             complement(3221..3373)
                     /locus_tag="P5335_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HRZ74848.1"
                     /translation="MAKKTVATLQTASKRLTKAIKMVKSPKTGAYTFVESVMAPELVD
                     SFLTKK"
     gene            complement(3382..3564)
                     /gene="rpmG"
                     /locus_tag="P5335_07960"
     CDS             complement(3382..3564)
                     /gene="rpmG"
                     /locus_tag="P5335_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRZ74849.1"
                     /translation="MAKKGNRIQVILECTEHKNSGVPGTSRYITTKNKKNTPDRLEIK
                     KFNPILKKVTIHKEIK"
     gene            complement(3589..3825)
                     /gene="rpmB"
                     /locus_tag="P5335_07965"
     CDS             complement(3589..3825)
                     /gene="rpmB"
                     /locus_tag="P5335_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRZ74850.1"
                     /translation="MSRVCDLTGKRAMVGNNVSHAMNKTKRKFSVNLVKKRFYLAEED
                     RWISLRVAASTIKTINKKGLAAVLKDAKSKGFIS"
     gene            complement(3900..5147)
                     /locus_tag="P5335_07970"
     CDS             complement(3900..5147)
                     /locus_tag="P5335_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HRZ74851.1"
                     /translation="MKATIITIGDEILIGQITDTNSGFIAKSLEKIGVQVHEMISISD
                     DKSHILETFAKVQNEVDFVIITGGLGPTKDDITKITFCDYFQDTLQRNSTVENHIVAL
                     FTKMNFNVSQVNRDQALVPSKAIVLQNQFGTAPGMWMTKEKTVFVSLPGVPYEMKAIV
                     TNELVPKIVSEYKRPYILHKTILTYGQGESIVAERIESWEDNLPGFVKLAYLPSPGRV
                     RLRLTARGSNEKILQTTINDLVEKLVEIIGDIIVGFEEDESIEVVLGELLSQKGLTLS
                     TAESCTGGKISEIITSISGSSNYFRGGIVSYATDTKVSLLGVKQTTIDAFSVVSTQVA
                     EEMALGVQKVLSSDFAVATTGNAGPSKGDADASLGTVCVAIATPNGVFSEQFNFGQPR
                     QKVIDRAVNKGLEMLLKEILKNY"
     gene            complement(5393..5737)
                     /locus_tag="P5335_07975"
     CDS             complement(5393..5737)
                     /locus_tag="P5335_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRZ74852.1"
                     /translation="MALNYNLAKVYALSDNDPEFVLQIITLFVSEIPEDLKFVKQGIE
                     EKNHKLAYSYAHKIKPTLDLLGMNVAYEEILQVEAWTKREGKRKEIKEIFKSIEDQVE
                     KAVKEINKDFDL"
     gene            complement(5741..6352)
                     /locus_tag="P5335_07980"
     CDS             complement(5741..6352)
                     /locus_tag="P5335_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRZ74853.1"
                     /translation="MKIICIGRNYVKHIEELQNERPDEPVVFLKPDSAILLKQHPFVI
                     PEFSDDIHHEVEILVRINKVGKYIDTKFASNYYDEIGLGIDFTARDLQAKLKEKGLPW
                     EKAKAFDGSAVIGNFLSKKDFSSTESINFELKSNGVTVQKGNTSHMLWKIDELIAYVS
                     QFFTLKIGDIIFTGTPEGVAKIKTNDILEGFIEEKKLFRIQVK"
     gene            complement(6401..7177)
                     /locus_tag="P5335_07985"
     CDS             complement(6401..7177)
                     /locus_tag="P5335_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRZ74854.1"
                     /translation="MELKLNRPICFFDLETTGVDVAKDRIVEISIFKVYPNGNKESKT
                     WLVNPEMKIPFLSTQVHGITDEKVVNEPTFKELSGQVYNMIKDSDLAGFNSDRFDIPL
                     LAEELLRSGVDFDMKSRVSVDVQTIFHKMEERTLSAAYKFYCGQSLENAHSAEADTMA
                     TYEILKAQLERYPDLVNDMKSLSEFTTRKKSVDFAGFIALNDKGQEIFSFGKHKGALV
                     EKVLEDEPGYFGWIQNADFPLYTKKVLTAIKLRKFNNKLN"
     gene            complement(7326..8684)
                     /locus_tag="P5335_07990"
     CDS             complement(7326..8684)
                     /locus_tag="P5335_07990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRZ74855.1"
                     /translation="MAKFELKLPKMGESVAEATITNWLKKVGDKIEVDEAVLEIATDK
                     VDSEVPSEVSGTLTEILFNVDDVVKVGQTIAIIDTEGGSVPIEVPKIEVLPAVNEIEK
                     SIEVAKEAVQITPIPVDFSSSEKFFSPLVKNIAKEEGVSLAELETIKGSGKEGRVTKD
                     DILAYVKDRTNQPVAVAPKVETVAVETPKADVKVDPVTSGPKTEPTPEKKIPVSVNGQ
                     DEIIEMDRMRKLISGYMTTSKQTSAHVQSFIEVDVTNIVNWRNKVKDAFEKREGEKLT
                     FTPIFMEAVAKAIKDFPMINISVEGDYIIKKKNINLGMAAALPNGNLIVPVIKNADML
                     NLVGLTKTVNDLANRARNNQLKPDEIQGGTYTVTNVGTFGSVFGTPIINQPQVAILAL
                     GAIRKVPAVIEAPTGDYIGIRHKMFLSHSYDHRVVDGSLGGQFVKRVADYLEGFSENR
                     EF"
     gene            complement(8811..>9336)
                     /locus_tag="P5335_07995"
     CDS             complement(8811..>9336)
                     /locus_tag="P5335_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRZ74856.1"
                     /translation="KINYYFCETSVIHYKGESTMKDGTYMKRFQEAMDYFYSKHFKKS
                     AFFSLFMKIGSFFFVFLKKNKKGVPNRRIDNYILISENELLKEKLEKLLGKNVKLLST
                     SFKNELPSMLKRTDTNVEVIFDQNNLSFSNIISAMEEFKTERITFKIIPHSCDFMIGS
                     NSSNDRGEVVMIEN"
BASE COUNT         2866 a         1720 c         1466 g         3284 t
ORIGIN      
        1 tcttttcgta attcatcgtt gagcataatt atgatttttt gttttccatg actgagttct
       61 tcacgcaaaa cggaggaagt caacgtaaca taaagtgtac ttcctttcaa aattacttct
      121 ttagtataag tgttcacacc atttcccatc aagtctttcc atgctttctc tacatcaatt
      181 ttatccatgc cggattggag tttattgttt tcaataaacg cttttagcac atcagaaata
      241 gtactttcgt tacttaatcg cttagccatt atttgatgga aaaaggggta aatcttttat
      301 aatgatattc caaattctgt ttatttttca acactaaaat actatctgtc aaagttataa
      361 tttcttcttt ccattgtgta tagggtgttt tataatgaat ttgaaaaatt ccggaactgt
      421 ctttgatttg aatttcctct aactcatcat ttatcaaaaa agttccatca aattgagggg
      481 ttactttttt tcgaaaaccg acgctttctt tccattcaaa ataatctact gtttcattta
      541 ttttgtattc tttcttttga ccatccggta attcaacttt ttgaatttcc cagtatccat
      601 ttattttaga aatagattca agagttactt tttcctgaca ggaaataaaa aatactaaaa
      661 taagtatgga ataaaagaaa tgtttcatcg tttcgtaaat aaaaatctaa aattacaatt
      721 aattttggag gaataaaaag taaatttgat taaacctttt aaagaaacaa aagtcttagg
      781 taagataaaa ctcagtacca tgaaaaaata tttttccttt ttgctaattt caatcctttt
      841 cttaaactgt tcttcagatt cagattctcc tgaagaaaac ccatcagatt ccatgtattt
      901 tcctcctatt tctggcacaa cttgggaaac taaatcgatg gctgacttgg gttggaatga
      961 aagcgaagta caaccccttt tgaattattt agaagaaaaa aataccaagg gattcatcat
     1021 tttggttaat ggtcgtattg tgatggaaaa ttattttaac gatcacaacg ccaatttacc
     1081 atggtattgg gcaagtgcag gaaaaacact tactactgca acaattggta ttgctcaaca
     1141 agaaggttta ctcagtatca atgataaagt atctgactat ttaggaattg gttggacatc
     1201 agcaccaaca aataaagaaa atttgattac taacaaacat ttactttcca tgtcatccgg
     1261 attagatgac tccttcggcg atggcaatga tcctattgac ctacaatata aagcagatgc
     1321 tggaacaaga tgggcctatc acaatgtatt tgaaaaatta caagatgtga ttgctaatgc
     1381 ctcaaatcaa acttttgatt cctattttac ttctaaactt aaaagtaaga taggaatgac
     1441 aggagcttgg attgagagtg atgaatttaa tgtttactgg agtacaacaa gaagtacagc
     1501 ccgttttggt cttttatcat taaacaaagg caaatggaat tcagaagtaa ttgttcccga
     1561 agcatacatg ttggaagcga tgtctacatc acaaactatt aataattcat acggttattt
     1621 atggtggttg aatggaaaag caaattatcg tttaccacaa acacaattac aatttccagg
     1681 aagtttaatc ccaaatggac cggctgatat ggtttgtgga cttggaaaaa atgatcagaa
     1741 aatttatgtt attccaagta aaaaaatggt tgttattcgc ttgggagatg tagcgaatcc
     1801 ggacaatcca acctttgcac tttcaggatt tgacaacgaa ttatggcaaa aaattaatgc
     1861 tgtaataaat tagtttccgt atttattttg aagaaaataa acccaatcgt actcataata
     1921 tcgagtaata attattaata cgacaccaag aatagcaatt gcccaagaat attttttata
     1981 aatcacggta taagcagtcc aaattccgaa aatcataaaa ggaattgcca taacaacatt
     2041 tggaatcatc caggctccac gaacaattgc taccgatttt aataaaacaa aaaagaaaca
     2101 gtatactgag acaatcttgg ttagggatac taaatactta ttgaaaactt taggctcttg
     2161 ttgttccatt atttaaaaaa agagtctaca aactcatatt tgttaaacac ttgtaggtct
     2221 tcaatacctt cccctacacc aatatattta acgggaattt ggaattgatc tgaaatccca
     2281 attacaactc cgcctttagc tgttccgtct aattttgtta cagctaaaca agatacctcc
     2341 gttgcagcag taaactctct agcttgctca aaagcatttt gaccggttga accatccaaa
     2401 actaataaca catcgtgtgg agcatcggct accacttttt gcatgactcg tttcacttta
     2461 gtcaactcat tcattaaatt cactttgtta tgcaaacgac cggcagtatc tataatgact
     2521 acatctgctc cttgtgtaac ggcactttgt aaagtatcaa aagcaacaga agctggatca
     2581 ctacccattt gttgcttcac aattggcaca cctactctat ccgcccaaat ttgcaattgg
     2641 tctatagctg cagctctaaa agtatcagca gctcctaaaa ccactttata tccagctttt
     2701 ttgaattgat acgctaattt gcctatagtt gtggttttac caacgccgtt gacacctaca
     2761 accataataa catatggttt tttatttgat ggaatatcaa attctgtcgc ttcaccggaa
     2821 ttggtttctg ataataatcc ggcaatttct tcacgaagaa ttttattgag ttcatcagta
     2881 cccatatatt tatcacgagc gacacgagct tcaattcgat caataatctt taaagtagtt
     2941 tttacaccta cgtcagaaga aactaacact tcttctaaat tatccaatac atcgtcatca
     3001 acagacgact ttccggcaac tgctttagtt agttttgcaa aaaaggaagt tttagtttgc
     3061 tctaggcctt tgtctaatgt ttcctttttt tcggatgaaa atattcgttt gaaaaaactc
     3121 atattctgat tttttaatat tttttattct gagaacaaat ataaaaataa aaaagctact
     3181 ctcaaggaaa gtagctttta ctataatgcg gtaaagtaaa ttatttctta gttaagaaag
     3241 aatctactag ctcaggagcc attacagatt caacaaaagt ataagctcct gtttttggag
     3301 attttaccat tttaatggct tttgttaatc tttttgacgc tgtttgtaac gttgctacgg
     3361 ttttctttgc catttcttat cttatttaat ttctttatga atagttactt ttttcaaaat
     3421 aggattaaat tttttaatct ctaatctgtc cggagtattc tttttgtttt tcgttgtaat
     3481 gtaacgagat gttcccggaa cacctgagtt tttatgctct gtacactcta aaattacctg
     3541 aattctattg cctttctttg ccatttttct ttatttttta gagttgaatt aagatataaa
     3601 accttttgat ttagcgtctt tcaacacagc agccaaccct tttttgttaa tcgttttaat
     3661 tgtagacgct gctactctta aagaaatcca tctgtcttct tcagcaagat agaaacgttt
     3721 tttaactaag ttcacagaaa attttctctt agttttgttc atcgcgtgag aaacattgtt
     3781 tcctaccatc gctcttttac ctgtaaggtc acaaactctt gacattatgc ttatctttta
     3841 tcgttattca aaatcagggt gcaaagaaac aaaaaagtaa tctattacac aaacaaattt
     3901 tagtagtttt ttaaaatttc tttcagaagc atttcaagtc ctttatttac tgctctatca
     3961 atcacttttt gacgcggttg accaaagtta aattgctctg aaaaaactcc gtttggagtt
     4021 gctatagcta cacagactgt acccaaagaa gcatcagcat ctccttttga aggtcctgca
     4081 tttccggtgg ttgcaacagc aaaatcagaa ctcaaaactt tctgtacacc taacgccatt
     4141 tcttctgcaa cttgagtact tactacagaa aaagcatcaa ttgtagtttg tttcacgcca
     4201 agtaaggaaa ctttggtatc tgttgcataa gaaacaatac caccacgaaa ataattggat
     4261 gaaccggaaa ttgaagtaat tatttcggat atttttccac ctgtacaact ttcggcggtg
     4321 gaaagtgtaa gccctttttg ggataataat tcccctagaa ccacttcaat actttcatct
     4381 tcttcaaagc ctacgatgat atcgccgata atttcaacta gcttttcaac taaatcatta
     4441 atagttgttt gcaatatttt ttcatttgaa cctcttgccg ttaaacgcaa acgaacacgt
     4501 cccggacttg gtaaataagc taatttcaca aaaccgggta aattatcttc ccaagattcg
     4561 atacgttccg ctacgatgct ttcaccttgt ccgtaagtta gtatcgtttt gtgcaatatg
     4621 taaggccgtt tatattcact tacaattttc ggaacaagtt cattggtgac aatagctttc
     4681 atctcatagg gaacaccggg taaagaaaca aatacagtct tttctttagt catccacatt
     4741 ccgggagcgg tcccgaattg attttgcaat acaattgctt ttgagggaac taaggcttgg
     4801 tcacgattaa cttgagaaac attaaaattc attttagtaa aaagtgctac aatgtgattt
     4861 tctactgttg aatttcgctg caaagtatcc tgaaaataat cacagaatgt tattttagtt
     4921 atatcatctt ttgtcggtcc caaaccacca gtaataatga caaagtctac ttcattctgc
     4981 accttggcaa aggtttctaa aatgtggctt ttatcatcgc taatggaaat catttcatgt
     5041 acttgaacgc caattttctc caatgattta gctataaaac cggaattggt gtccgtaatt
     5101 tgtccgataa gaatttcgtc gccgatggtg atgatagttg ctttcatgtt gggaggtgct
     5161 aagtaactaa gggtttgagg cactaaggct ctgagattaa ttttgctaaa ttactcaact
     5221 tataatgaac tctgagtctt agctaccttc tagcccggat agtagcggta agcccggagc
     5281 taaaaaaact aatttttgtt tgcaaaaaag agcgactaac ggaagctcat tttttggctt
     5341 agaaaaatag gttttttagt gagaacttgt agcggaaagc cggaatagct tcttataaat
     5401 cgaaatcttt gtttatttct ttgacggcct tttcaacttg gtcttcaatt gacttaaaga
     5461 tttctttgat ttctttgcgt tttccttctc gttttgtcca tgcttctacc tgaagaattt
     5521 cttcataagc aacgttcatt cctaataaat caagtgttgg cttaattttg tgagcatagc
     5581 tgtaggctaa tttgtgattt ttttcctcga tgccttgttt gacaaacttt aaatcctccg
     5641 gaatttcgct aacgaataag gtaattatct gtaaaacgaa ctcggggtcg ttgtctgaaa
     5701 gtgcatacac tttggctagg ttgtagttta atgccatttt ttattttact tgaattcgga
     5761 ataatttttt ttcttcaata aatccttcta ggatatcgtt tgtttttatt tttgccacac
     5821 cttccggtgt tcctgtaaaa ataatatcgc caattttaag tgtaaaaaac tgagaaacat
     5881 aagctataag ttcatcaatt ttccacaaca tgtgactcgt gttccctttt tgaacggtaa
     5941 ctccgttaga tttcaattca aaattaatac tttctgtgga actgaaatct tttttcgata
     6001 aaaagttacc aataaccgct gaaccgtcaa aagctttcgc tttttcccat ggtaaaccct
     6061 tctcttttaa tttcgcctga aggtcgcgag cggtaaaatc tataccgagt ccgatttcgt
     6121 cataataatt tgaggcaaac ttagtatcga tgtatttacc aactttgttg attcgcacta
     6181 aaatttccac ttcatggtga atatcgtcac taaactcagg aatcacaaat ggatgttgtt
     6241 taagtaaaat agcactatcc ggttttaaaa aaacgacagg ttcatccgga cgctcatttt
     6301 gaagctcctc tatgtgttta acataatttc ttccgataca gattattttc atttttagag
     6361 gtgctgatga actaaatatt ctatacttgc taataggatt ttagtttaac ttgttattaa
     6421 actttctgag tttgattgcg gtgagcactt ttttggtata caatggaaaa tctgcatttt
     6481 gaatccaccc gaaatatccc ggttcatctt ctaacacttt ctcaacaagg gctcctttat
     6541 gctttccgaa ggaaaaaatc tcttgacctt tatcattaag agcaataaat cctgcaaaat
     6601 cgacagattt tttccgagtt gtgaattcag ataacgattt catgtcattc actaaatctg
     6661 gatatcgttc taattgtgct ttgaggattt cgtaagttgc cattgtatct gcttcagcag
     6721 aatgtgcatt ttcaagtgat tgaccgcaat agaatttata agctgcactc aaagttcttt
     6781 cttccatttt gtgaaatatg gtttgaacat cgaccgaaac tctacttttc atatcaaaat
     6841 caactcctga acgcaacaat tcttcggcta aaagcggaat atcaaatcga tccgaattaa
     6901 atccggctaa atctgaatct ttaatcatat tgtaaacctg accggaaagt tctttgaaag
     6961 taggctcatt tactactttt tcgtcagtaa ttccgtggac ttgtgtggac aaaaacggaa
     7021 ttttcatttc cggattgacc aaccatgttt tactttcttt gtttccgttt ggataaactt
     7081 taaagattga gatttccaca attctgtctt ttgcaacatc aacacctgtc gtttcgaggt
     7141 caaaaaagca gatgggtcta tttaatttaa gttccatttt aaaattttag gagagcaaat
     7201 ataagggatt gaaatgacct ttagtgggtt gttttcgttt ttttaaaata cttttaataa
     7261 ataaaattaa aaaacccttt cagagttcaa aacactaaaa gggttagttt ttatataaaa
     7321 ataatctaaa attctctgtt ttcgctgaag ccttctaaat aatcagctac tcgtttgacg
     7381 aattgtccac ccaaagaacc atctaccaca cggtgatcat aactgtgaga taagaacatt
     7441 ttgtgtcgga ttccgatata atctccggtt ggagcttcaa ttacagccgg aactttacga
     7501 attgctccta gtgctaaaat cgccacttga ggttggttaa taatcggtgt tccgaaaacg
     7561 cttccaaatg ttccaacgtt ggtaactgta taggttccac cttgaatttc atctggtttt
     7621 aattgattat ttcgagctcg gttagccaaa tcatttacgg ttttggttaa tccgacaagg
     7681 tttagcatat ctgcattttt gataacaggt acaattaaat ttccatttgg taatgcagct
     7741 gccattccta aattgatgtt tttctttttg atgatgtaat cgccttcaac agaaatatta
     7801 atcattggga agtctttgat ggcttttgcc acggcttcca taaatattgg ggtaaatgtt
     7861 agcttctcgc cttctctttt ttcaaaagca tctttaactt tatttctcca attcacaata
     7921 tttgttacat ccacttcaat aaacgactgc acgtgagccg atgtttgctt ggatgtggtc
     7981 atataacccg atatcaattt tcgcatacgg tccatttcga taatctcgtc ttgaccgttt
     8041 acggaaaccg ggattttttt ctctggggtt ggttctgttt ttggtcccga agttacggga
     8101 tcaactttta catctgcctt tggggtttcg acagcaacag tttcaacttt tggagctact
     8161 gcaaccggtt gatttgttct gtcttttacg taggctaaaa tatcatcttt ggttacacga
     8221 ccttccttac cggaaccttt aattgtttct aattctgcaa gagaaacacc ttcttcttta
     8281 gcaatgtttt ttacaagagg agaaaagaac ttttctgatg aagagaaatc aactggaata
     8341 ggagttattt gtacagcctc ttttgcaact tcaatacttt tttcaatttc attaactgcc
     8401 ggtaaaactt caatttttgg aacttcaatt ggaacacttc cgccttcggt atcaataata
     8461 gcaatcgttt gtccaacttt gacaacatcg tcaacgttga aaagtatttc agtaagtgtt
     8521 cccgaaacct cagatggaac ttcgctatct actttatcgg tagcaatttc caagactgct
     8581 tcgtcaactt caattttatc tcctactttt ttaagccaat tagtgatggt cgcttccgca
     8641 acactctctc ccattttggg caatttcaat tcaaattttg ccatatcgtt aacctaaagg
     8701 tttattttgt gtatcagttt gcgaaattaa taaaaaaagg attgatttta ttaattatca
     8761 ataaaaatat tcaatatcaa aaatcaatta taatgtcaat gtcaaattca ttaattttca
     8821 atcattacta cttcacctct atcattgcta ctattgctgc caatcatgaa atcacatgaa
     8881 tggggaataa tcttgaaagt tatcctttcc gttttaaatt cttccatagc agaaataatg
     8941 tttgaaaaag ataagttatt ctgatcaaaa atcacctcaa catttgtatc cgttcttttc
     9001 aacatcgaag gtaattcatt tttaaaagac gttgaaagga gtttcacatt ttttcctaac
     9061 aatttttcga gtttttcttt taataactca ttttcggaaa ttaaaatata attgtcaatt
     9121 cttctattgg gaactccttt tttatttttc ttcaaaaaaa caaaaaagaa actccctatt
     9181 ttcatgaata agctaaaaaa agctgatttt ttaaaatgct tagaataaaa ataatccatg
     9241 gcctcctgaa aacgtttcat ataggttccg tctttcatcg tgctttcacc tttgtagtgt
     9301 atcactgaag tttcgcaaaa ataatagttt atttta
//
LOCUS       DAOTCW010000089         9255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_5072_length_9255_cov_44.877609, whole genome shotgun sequence.
ACCESSION   DAOTCW010000089 DAOTCW010000000
VERSION     DAOTCW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5072_length_9255_cov_44.877609"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..458)
                     /locus_tag="P5335_08000"
     CDS             complement(21..458)
                     /locus_tag="P5335_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRZ74857.1"
                     /translation="MSKVEDFLTTADEQEIVLAIAMAEKNTSGEIRVHIENHSETPPI
                     ARAQEVFNFLEMFKTEARNGVLFYVGVADKKFAIIGDIGIDDVVEDDFWDCTKDIVIE
                     NFKNKKYKEGLVAGITRAGERLKEFFPYASDDTNELSNEISKG"
     gene            complement(462..1046)
                     /locus_tag="P5335_08005"
     CDS             complement(462..1046)
                     /locus_tag="P5335_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRZ74858.1"
                     /translation="MRKWLIPVGIIIGLIFIIGFYVVGIQNTAITKSQAVNKSWGDVQ
                     TSYQRRSDLIGNLVNTVKGAADFEKSTLEAVISARARATQTTIDPTNVTSEQLAEFNK
                     AQSGVSSALSRLLVTVEAYPELKANQNFLKLQDELASTENTIQTARVRFNESVEFYNN
                     HILKFPNNLFLGKYKEKPYFDAEPGSEKAPEVKF"
     gene            complement(1127..1459)
                     /locus_tag="P5335_08010"
     CDS             complement(1127..1459)
                     /locus_tag="P5335_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRZ74859.1"
                     /translation="MQIELSKDKLYFSIGEIADAFGVNASLVRFWDKEFDILKPKKNA
                     KGNRMFSQEDVKNLQLIYHLVKERGFTLEGAKTHLKEGQKKTLDKYEIISKLEGIKEQ
                     LQQIKGEM"
     gene            complement(1523..2503)
                     /locus_tag="P5335_08015"
     CDS             complement(1523..2503)
                     /locus_tag="P5335_08015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRZ74860.1"
                     /translation="MSKKVKYYYDTESLAYKKISPKKGRKFGYVALFLVSSALFGFLC
                     FVILLNTPYFETPKDRLQAREIENLKLQYALLNKKMDQLDEVLVSLEDRDNNLYRVYF
                     NSSPIPTEQRQAGFGGVNRYKELEGFDNSELVINTTKRVDIISKQLAIQSKSLDEILS
                     LAKGKEKLLAAIPAIQPVKNEDLTRMASGFGTRNDPFTKIRKMHYGMDFTAKTGTPVF
                     ATGDGVVEKSDNTLSGYGNLLIIKHGFGYTTYYAHLSKYKVRAGQKVKRGDIIGYVGS
                     TGRSEAPHLHYEVHKDGEPINPINFYYGSISAAEYIAISKIANQENQALD"
     gene            2734..5382
                     /gene="alaS"
                     /locus_tag="P5335_08020"
     CDS             2734..5382
                     /gene="alaS"
                     /locus_tag="P5335_08020"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRZ74861.1"
                     /translation="MKSQDIRKQFLQFFESKGHLIVPSAPIVLKDDPTLMFNNSGMAQ
                     FKEYFLGNAVPKSKRIADTQKCLRVSGKHNDLEDVGFDTYHHTMFEMLGNWSFGDYFK
                     KEAIAWAWEFLTEVLKLDKDRLYVSVFEGNPQENVPFDQEAFDLWKQYVAEDRIILGN
                     KKDNFWEMGDQGPCGPCSEIHIDLRSNEERAKVSGKSLVNNDHPQVVEIWNNVFMEFN
                     RKADGSLEKLPAQHVDTGMGFERLCMAMQGVQSNYDTDVFTPIIREIETITGTKYIPL
                     SGAEGRIASAEEEKINIAIRVVADHVRAVAFAIADGQLPSNTGAGYVIRRILRRAIRY
                     GFTFLGTKEPFIYKLVETLSNQMGEFFPEIKAQKLLCANVIREEEASFLRTLDQGLVL
                     LDSIISNTTGTIVSGKKAFDLFDTFGFPIDLTALILREKGFTLDEEAFNSELKKQKER
                     SRAASQVKAGDWQILVEDAEEEFIGYDNLEGLVKITRYRKVDSAKDGEVFQLVFNYTP
                     FYPEGGGQVGDRGAIESPDGDVIYVIDTKKESNLIIHYTKSLPKDLSATFKALVGPNL
                     RARTEANHSATHLLHQALRSILGTHVEQKGSMVNEHNLRFDFSHFSKVTSEELVEIEQ
                     FVNARIRENLDLVEKRSMTYQAAIEEGAIALFGEKYSDVVRAIRFGESMELCGGTHVK
                     NTADIWYFKIISEGAVAAGIRRIEAITNVAVKEYFLNQSETVNEIKSVLKNAQDPVKS
                     IVTLQDENSKLKKQIELLLREKAKHLKGELASELQDFNGIKFLAKQVDLNAEGAKDLA
                     YELGNLGDNLFLVLATAEDEKPMLTCYISKELVASKNLNAGQVVRELGKYIQGGGGGQ
                     PFFATAGGKNVNGIKEALEKAKDYLN"
     gene            5389..>5810
                     /locus_tag="P5335_08025"
     CDS             5389..>5810
                     /locus_tag="P5335_08025"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HRZ74862.1"
                     /translation="MNFRTQITIPKTNHPIDYNSKVISIGSCFAATISTKLEYFKFQQ
                     YGNPFGILFQPNAIENLLTRAVENRLFTEDDLVFENELWHCLEVHSELSNSDQSDMLI
                     NLNGILTFLCGQLQEATHLVITFGTAWTYRYLETQKRVA"
     assembly_gap    5811..5910
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5911..6222
                     /locus_tag="P5335_08030"
     CDS             <5911..6222
                     /locus_tag="P5335_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HRZ74863.1"
                     /translation="VEQKKVHYFPAYEIMMDDLRDYRFYTEDMLHPNETAIQYIWGIF
                     MEAHISYHAFPIMNELDSIQKGLAHRPFNPNTQQHQLFIQKLHERIEQLQAKHPFLGL
                     F"
     gene            6341..6721
                     /locus_tag="P5335_08035"
     CDS             6341..6721
                     /locus_tag="P5335_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74864.1"
                     /translation="MTKKLTFIKAIIITFLLLNVYSVVAQKPVLTDEEVVIETVTKEI
                     DDVFVSKEFLNKKNKKFTDIKGTMVVDIGIVQNGKVSSFFKVESDIKDIDFINFMSDY
                     ILNHKFNFKLQKQQRYKIRYTITF"
     gene            6748..>7514
                     /locus_tag="P5335_08040"
     CDS             6748..>7514
                     /locus_tag="P5335_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRZ74865.1"
                     /translation="MKRITLLMLTISMMMTLPTSAQLGKLVDKVKGTGKTAGKKSGIF
                     TTVWEAEFENKADRLAVSNGDGSYIIGTDDNSATVLDANGKLVWSGDYKKITTNKTNK
                     CEYQYTIWKKNGGYLFLFDERKLGTDLVAVIDIPTGKELWNSEKFQNLIPKSGQSGIE
                     GSDQGELETVKYIDELDAFLISQKESVILVKADTGEKIWETNRFKGAVGKYIYDAKRN
                     EIIMVNFKPTALGALFAGFKNQLVKINAVNGDVLWDAT"
     assembly_gap    7515..7614
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7829..9253
                     /locus_tag="P5335_08045"
     CDS             7829..9253
                     /locus_tag="P5335_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74866.1"
                     /translation="MKKSIVYIVLLVAFAVHAQEIQWASKLIKFSSDLGGKQNGIKRI
                     LGKPDIFPQGGSSPNAWSPKKALDGKEVVEVGFEKPQTIKQIAIFENLNAGCVVKVSV
                     DNGSGKYETVWSRKPNWQTPVYKRTFAADRSYYFGRKRRKIEKAPEVNLNPGIEYIIL
                     EKEVANGIALKIEFNFALLHGQKQIDAIGISNSALPIQPTINEISFSDKIQEVEVVPL
                     KGFYPSSPVISTDGKKLIFSSLSDEKEEVFICQKGPDGKWSNPTKEETLSQNSYYNYI
                     EHYGNDFILKGGIGYNKGTQESGYEILKLQSEHYQSQGALKIAAYNNYDETSEATISS
                     DGTILILGIETDFTHGGSDLYFSKKKEDGTFGFLQNMGKMINSAADEGMPQLLSDNKT
                     IVFSTNGFSGYGEYDIYVSYRLDETWKNWSEPQNLGKKINSNDFDGLPFYDERDEKLY
                     FIQAIDGIQYLKQVTIPKSDLMKS"
BASE COUNT         3066 a         1431 c         1651 g         2907 t
ORIGIN      
        1 atttttgaag taaatttcat ttatcctttg gatatttcat tagaaagttc gttcgtgtca
       61 tctgatgcgt agggaaagaa ttcttttaat ctttctccgg ctctagtaat tccggccact
      121 aaaccttctt tgtatttttt gtttttgaag ttttctatga caatgtcttt ggtacaatcc
      181 caaaaatcat cttctacaac atcatcaata ccaatgtctc caatgatagc aaacttttta
      241 tctgcaacac ctacataaaa caacacaccg tttcgggctt ccgttttaaa catttcaaga
      301 aaattgaaca cctcctgagc tctcgctatt ggaggtgttt ctgaatgatt ctcgatgtgt
      361 acccgaattt ctccggaagt atttttttct gccattgcaa ttgccagaac aatttcctgt
      421 tcatctgcag tggttagaaa atcttctact tttgacatag tttagaattt aacttccggt
      481 gctttttcag aacccggctc tgcatcgaaa tatggttttt ctttatattt tcctaagaat
      541 aaattgtttg gaaattttaa aatatgatta ttgtaaaatt caacagattc attaaaacgc
      601 actctggctg tttgaattgt attttcagta cttgccaatt catcttgtaa ttttaagaaa
      661 ttttgattgg ctttcagttc aggatatgct tcaacagtaa ctaacagtct tgacaaagca
      721 gaagaaacgc cgctttgagc tttgttaaat tcggctagtt gctctgaagt tacattagta
      781 gggtcaatgg tagtttgcgt agctcttgct cttgcagaaa taacggcttc cagagtactt
      841 ttttcaaaat cggcagctcc ttttaccgtg tttactaaat tgccaatcaa atcacttctt
      901 ctttggtaag aggtttgaac atctccccaa gatttattga ccgcttggct tttagtgatt
      961 gctgtatttt gaattccaac tacatagaat ccaataatga atattaatcc aataataata
     1021 cctacgggaa ttaaccattt tctcataatt tctgtttttt aatttatgta ttagtctatt
     1081 gagtgagcaa tgcgttacaa tgatttttat ttttgcctat tgcaatttac atttctcctt
     1141 tgatttgctg caattgttct ttaattcctt ctaattttga aatgatttca tacttgtcta
     1201 aggttttctt ttgaccttct ttcaaatgcg ttttggctcc ttccaaagta aacccccgtt
     1261 ctttaaccaa atgataaatt aactgaaggt tcttcacatc ttcttgcgaa aacattcgat
     1321 ttcctttggc attttttttg ggtttgagga tatcaaattc tttatcccaa aaacgaacca
     1381 gagaagcatt tacgccaaat gcatcggcta tttctccaat actgaaatat aatttgtctt
     1441 tagaaagttc aatttgcatc gttttttatt tttttaattc tattctgcta ctaagataaa
     1501 caaaataacc tttatttaaa ccttagtcaa gagcttgatt ttcctgatta gcaatttttg
     1561 aaatagcaat atattcagct gctgaaatgc ttccgtaata aaaattaatc ggattgattg
     1621 gttccccgtc tttgtgcact tcataatgta aatgaggagc ttcgcttcgg ccggtactac
     1681 ctacatagcc tataatatct cctcgtttta ctttttgacc ggctctcact ttatatttgc
     1741 ttaaatgtgc ataataagtg gtgtatccaa aaccatgttt gataattaat aaatttccat
     1801 acccagacaa agtattgtca gacttttcga caactccatc gcctgtagca aaaaccggtg
     1861 tgcctgtttt agcggtgaaa tccattccat aatgcatttt tctgattttc gtgaaagggt
     1921 cattcctagt tccaaatccg gaggccattc gggttaaatc ttcattttta acaggttgaa
     1981 ttgccggaat agcggctaac agtttttctt ttccttttgc taatgataaa atttcatcca
     2041 atgatttgga ttgaatggct aattgtttgg aaataatatc tacccgttta gtggtgttta
     2101 taaccaattc agaattatca aatccttcca attctttgta gcgattaact ccaccaaaac
     2161 ctgcttgtcg ttgctcggtt ggaataggag aagagttaaa ataaacgcga tataaattgt
     2221 tatcacggtc ttctaaactt actaaaactt catccaattg atccattttt ttgtttaaaa
     2281 gggcatactg aagttttaaa ttctcaattt cccgagcttg taaacgatct tttggcgttt
     2341 caaagtaagg cgtgttcaat aaaatgacaa aacataaaaa tccaaataaa gctgaagaaa
     2401 ccagaaacaa cgccacataa ccaaacttcc gacctttttt agggctgatt tttttataag
     2461 ctaaactttc ggtatcgtaa taatacttta ctttcttcga catttcttaa attatactat
     2521 ttttgtgctt tcaaaaggat tagacgaaca aatttatgaa atgtttcatt ctaaagctaa
     2581 tttaatttga cctccaagtt aatattagta ctgaaaaata aatgaatttt aactgtttaa
     2641 aatctgagaa aataataaaa ttaaaataaa ctttgaaact ttataacttg gttccatgat
     2701 ggcactaagg agtaaattca aaaatcaaat acaatgaaat cacaagatat ccgcaagcag
     2761 tttctgcaat tttttgaaag taaaggacat ttaatcgttc cttcagctcc cattgttcta
     2821 aaagacgatc caacgttgat gtttaataac tcaggcatgg ctcagtttaa agagtatttt
     2881 ttaggaaatg cagtgccaaa aagcaaacga attgcagata ctcaaaaatg tttgcgggtt
     2941 tccggaaaac acaatgattt ggaagatgtt ggttttgata cctatcacca taccatgttt
     3001 gaaatgttgg gaaactggtc ttttggcgat tatttcaaaa aagaagccat tgcttgggct
     3061 tgggagtttt tgactgaagt tttaaaatta gacaaagacc gattatatgt ttccgtgttt
     3121 gaaggaaatc cacaagaaaa tgtgccattt gatcaggaag cctttgattt atggaaacaa
     3181 tatgtggcag aagatcgtat tatcttagga aataaaaaag ataatttttg ggaaatgggc
     3241 gaccaaggac catgcggtcc atgttcagaa attcatatcg atttacgttc aaatgaggaa
     3301 agagctaaag tttccggaaa gagtttagtg aacaatgacc atccgcaagt ggtggaaatt
     3361 tggaataacg ttttcatgga atttaatcgt aaagccgatg gaagtttaga aaagctacca
     3421 gcccaacacg tagataccgg catgggattt gaacgattat gtatggcgat gcaaggtgtg
     3481 caatctaatt atgataccga tgtttttacg ccaattatta gagaaattga gaccattacc
     3541 ggtacaaaat atattccact tagtggagct gaaggaagaa ttgcttctgc tgaggaagaa
     3601 aaaatcaata ttgccattcg cgttgtagca gaccatgtac gggctgtcgc ttttgccatt
     3661 gccgatggac aattgccttc taacaccggg gcaggttatg ttattcgtag aattttgcga
     3721 agagccattc ggtacggatt tactttttta ggaaccaaag aaccttttat ttataaatta
     3781 gtggaaacac taagtaatca aatgggtgaa tttttccctg aaatcaaagc acaaaagtta
     3841 ttatgtgcca atgtgattcg agaggaagaa gcttcttttt taagaacgtt agaccaagga
     3901 ctcgttttat tggattctat catttccaat actaccggaa ctattgtctc cgggaaaaaa
     3961 gcattcgatt tgtttgatac atttggattt ccaatcgatt taacagcctt aattcttcgt
     4021 gaaaaaggat ttactcttga cgaagaagcc ttcaattcag aactaaaaaa gcaaaaagag
     4081 cgttcacgtg cagcttcaca agtaaaagcg ggagattggc aaattttggt tgaggatgct
     4141 gaagaagaat ttattggcta cgataattta gaagggttag taaaaattac gcgatacaga
     4201 aaagtagaca gtgccaaaga cggtgaagtt tttcaactgg tttttaatta tacccctttt
     4261 tatcctgaag gtggaggtca agtgggcgac agaggagcta tagaatcacc ggatggggat
     4321 gttatatatg ttattgatac taaaaaagaa agtaacctca ttattcatta tacgaaaagt
     4381 ctgcctaagg atttatcagc tacttttaaa gctttagttg ggccaaattt acgtgctcgt
     4441 acagaggcaa accattcggc aacccattta cttcatcaag cattacggtc tattttaggg
     4501 actcatgtgg aacaaaaagg ttcaatggtt aacgaacata atttgcgatt tgacttttca
     4561 catttttcga aagtaacctc ggaagaatta gtagaaattg aacagttcgt taatgctaga
     4621 attcgtgaaa atttagattt agttgaaaag cgttccatga cttatcaagc ggcaattgaa
     4681 gaaggagcta tcgctttatt tggcgaaaaa tattcagatg tagttcgagc tattcgtttt
     4741 ggcgaatcga tggaactttg tggtggaaca catgtgaaaa acactgctga tatttggtat
     4801 tttaaaataa tatctgaagg ggctgttgct gccggaattc gtcggattga agccattact
     4861 aatgtggcag taaaagaata ctttttgaat caatccgaaa ctgtaaacga aatcaaatcg
     4921 gtattaaaaa atgctcaaga cccggtaaaa tcaattgtaa ctttgcaaga tgaaaactcg
     4981 aaattaaaga aacaaataga acttttactt cgtgaaaaag caaagcatct taaaggtgaa
     5041 ttagcctctg aattgcaaga tttcaacgga attaaatttt tagcaaaaca agttgattta
     5101 aatgctgaag gagctaaaga tttggcctat gaattaggca atttaggaga taatttattt
     5161 ttagtattgg ctacagctga ggacgaaaaa cccatgttaa cctgctatat ttcgaaagaa
     5221 ttggtagcat ctaaaaacct aaatgccgga caagtggttc gtgaattagg aaaatacatc
     5281 caaggaggtg gtggtggcca accatttttt gcaacagcgg gaggaaaaaa tgttaatgga
     5341 attaaagaag cattggaaaa agcaaaagac tatttgaact aatttttaat gaatttcaga
     5401 actcaaatca ctatacctaa aacgaatcat cccatcgatt ataattcgaa ggtgatttcg
     5461 ataggttcgt gttttgccgc aacgataagt actaaattag aatatttcaa atttcaacaa
     5521 tacggcaacc catttggaat attgtttcag cccaatgcca ttgagaattt attgaccagg
     5581 gcagttgaga atagattatt taccgaggat gatttagttt ttgaaaatga actttggcat
     5641 tgcctggaag tacattcgga acttagtaat tcagatcagt ctgatatgtt gattaattta
     5701 aatggaatct tgacgttttt atgtggccaa cttcaagaag ctacacattt ggttattaca
     5761 tttggtacgg cttggactta tcgttattta gaaactcaaa agagagttgc nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtggaacaaa aaaaagtgca ctatttcccg
     5941 gcttatgaaa tcatgatgga tgatttaaga gattatcgtt tttatacaga ggatatgcta
     6001 catccaaatg agaccgccat tcagtatata tggggaattt ttatggaagc tcatatctct
     6061 tatcacgcct tcccaatcat gaatgagttg gatagtattc aaaaaggatt agcccatcga
     6121 ccttttaatc caaacacaca acagcatcaa ctttttattc aaaaactaca cgaacggata
     6181 gagcaattgc aagcaaagca tccttttttg ggtctttttt aaaatcataa ttaattacgt
     6241 ttttctaatc ctattcttct tttcaaaata gggcaaatgc cgtatggttt gaaattataa
     6301 aataattcaa ttttggtcat tattagtaac acaataaatc atgactaaaa aattgacttt
     6361 tattaaagcg attattataa cattcttact actgaatgtt tattctgtgg tagcacaaaa
     6421 accggtttta acggatgaag aagttgtaat agaaacggta acaaaagaaa tagatgatgt
     6481 ttttgtttcc aaagaatttc taaacaagaa aaacaaaaaa tttactgaca ttaaaggaac
     6541 catggtggtc gatatcggta ttgttcaaaa cggaaaagtt tcctcctttt tcaaagtaga
     6601 aagtgatatt aaagacatcg atttcataaa ctttatgtca gactatatct tgaatcacaa
     6661 attcaatttc aaacttcaaa aacaacagcg atataagatt cgctatacca taacatttta
     6721 aaaaaaccta actaaaattt atacaaaatg aagagaatta cactattgat gttaaccatc
     6781 tctatgatga tgacattgcc aacttcggca caattaggaa aactggttga taaagttaaa
     6841 ggaacgggaa aaacagccgg aaaaaaatca ggaattttca caacggtttg ggaagctgaa
     6901 tttgaaaata aagcagatcg tttagcggtt agcaacggag atggttcgta cataattggt
     6961 acagatgaca attccgcaac ggttttagat gccaatggaa aattagtttg gagtggagat
     7021 tataaaaaga taacgactaa taaaacgaac aaatgtgaat accaatatac tatttggaag
     7081 aaaaatgggg gctatctttt tctatttgat gaacgaaaat taggaacaga tttagtagca
     7141 gttattgata ttcccaccgg aaaagaatta tggaattctg aaaaatttca aaatttaatt
     7201 cctaaatctg gtcaaagtgg aattgaaggt tctgatcaag gggaattaga aacggttaaa
     7261 tatattgacg aattagatgc ttttttaatt tctcaaaaag aatctgtcat tttagtcaaa
     7321 gctgatacag gagaaaaaat ttgggaaacc aatcgtttta aaggagctgt gggtaaatat
     7381 atttatgatg caaaacgcaa cgaaattatc atggtaaatt ttaaaccaac tgccttagga
     7441 gctttatttg cagggtttaa gaatcaatta gtaaaaataa atgcagttaa cggagacgtt
     7501 ttatgggatg ccacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatgacc
     7621 aatggagaag tcaagggttc agttcaatcc aaaggaaaag gaggtagccc aaaatttggc
     7681 gacggaattg acattaccac tgacggagaa tatatttttg cctttaaagg gaaaaaagta
     7741 gagaaaatca aagtaaataa ctaggtgagc tttagtttaa ttgtgaaaga gctgtgattg
     7801 tattacagct cttttaagtt ttgaaaagat gaaaaaaagt attgtctata tagtattatt
     7861 agtggctttt gccgttcacg cacaagaaat acaatgggct tcaaaactca ttaagttttc
     7921 ctctgatttg ggtgggaaac aaaatggtat caaacgtatt ttaggaaaac cggatatttt
     7981 tccacaagga ggaagtagtc caaatgcttg gtcgcccaaa aaagcgttag atggaaaaga
     8041 agtcgttgaa gttggatttg aaaaaccaca aaccattaaa caaattgcca tttttgaaaa
     8101 tttaaatgcg ggttgtgtcg tgaaggtttc cgttgataac ggttccggaa aatatgaaac
     8161 cgtttggtcc agaaaaccaa attggcaaac gccggtttac aagagaacat ttgctgcaga
     8221 cagaagttat tattttggca gaaaacgcag aaaaattgaa aaagcaccgg aagttaattt
     8281 aaatcccgga attgaatata ttattttaga gaaagaagtg gctaatggaa ttgcacttaa
     8341 aatcgaattt aattttgcct tacttcatgg tcaaaaacaa attgatgcga ttgggatttc
     8401 taattcagca ctcccaattc aacccacaat caacgaaata tcattttcag ataaaattca
     8461 ggaagtagaa gttgtccctt taaaaggatt ctatccgtca agtccggtta tttcaacaga
     8521 cggaaaaaaa ttaatattct cctccttatc agatgaaaaa gaggaagtgt ttatttgtca
     8581 aaaaggtccg gatgggaaat ggtcaaatcc tactaaagaa gagacattga gtcaaaattc
     8641 ctattacaat tatatagaac attatggaaa tgattttatt ttaaaaggag gaattggtta
     8701 taacaaagga acccaagaat ccggttatga aattttaaaa ctacaaagcg aacattatca
     8761 atcacaagga gctttaaaaa ttgctgctta taacaattat gatgaaactt ctgaggctac
     8821 aatttcttca gacggtacta ttttaatttt aggaatagaa acagatttta ctcatggtgg
     8881 ttctgattta tacttttcaa aaaagaaaga agatggcact ttcggatttc ttcaaaacat
     8941 gggtaaaatg attaattctg ctgccgatga aggaatgcca caattgcttt cagataataa
     9001 aactattgtt tttagcacta atggattcag tggctatggt gaatatgata tttatgtttc
     9061 ttaccgtttg gacgaaactt ggaaaaattg gtctgaacct caaaatctgg gtaaaaaaat
     9121 aaattcaaat gattttgatg ggttgccttt ttatgatgaa agagatgaaa aattatattt
     9181 tattcaagca atagatggta tacaatattt aaagcaggtg actattccaa agtcagattt
     9241 aatgaaaagt taaaa
//
LOCUS       DAOTCW010000090         9058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_5259_length_9058_cov_44.619349, whole genome shotgun sequence.
ACCESSION   DAOTCW010000090 DAOTCW010000000
VERSION     DAOTCW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5259_length_9058_cov_44.619349"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1243)
                     /locus_tag="P5335_08050"
     CDS             complement(8..1243)
                     /locus_tag="P5335_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRZ74867.1"
                     /translation="MKLEYFIAKRLTTAKDHKSSISKPIIKIAITAIAIGIIMMIVSV
                     ATGLGLQQKIRQKVSAFNGHIIISNFDDNQSEVSKVPVSNKQDFYPKFTSVEGVNHIQ
                     AIASKAGIIRTEKAFEGIIFKGVGSDYKWENLTEYIVDGKSPNVSRDLNPEVLISQYL
                     ANRLYLKVGDKFNTFFMKEDSNEMPNLRVFTISGIYNSGFQEFDATYILGDIRHVQRI
                     NRWQPDEVGAFEVFIDDFTKIEQKGEEVYEQTSYMLDSKTIIDKYFYIFEWLQLFDFN
                     IIVILVIMILVATINMVVALLVLILERTQMIGILKALGADNWMVRKIFLFNAFHIILR
                     GLLWGNCIAIALMLIQQQFGVIKLNPENYYVSEAPIYFNWFYIFLLNLGTIIISFLVL
                     LVPSYIITKISPVKAIRFD"
     assembly_gap    1423..1522
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1523..2676
                     /locus_tag="P5335_08055"
     CDS             <1523..2676
                     /locus_tag="P5335_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRZ74868.1"
                     /translation="AVSTVDIITGAENFSAYLPLLQDKKVGVVTNQTGIVNYKIPYST
                     TIADGKPLRPITKHLVDYLLEHGITVEKIYAPEHGFRGVADAGELIVDGKDSKTGLPI
                     ISLYGDNKKPKPEQLEGIDILLFDLQDVGARFYTYISSLHYVMEACAEINLPLIILDR
                     PNPNGAIVDGPILEKEFTSFVGMHPIPVLHGLTIGEYGKMINGEKWLKDSIQCNLTVI
                     PCLHYRRDMDYSLPVKPSPNLPNDQAINLYASLCFFEGTNVSVGRGTDKQFQIYGSPF
                     LPQGDFSFMPLPNLGSKNPLHNNVVCHGEDLSEISPVKRLRLKWLIEAYENTADKSTF
                     FTPFFTKLAGTKLLRVQIEQGLSEKEIRNTWEKGLEEFLEMRKTYLIYN"
     gene            complement(2678..3067)
                     /locus_tag="P5335_08060"
     CDS             complement(2678..3067)
                     /locus_tag="P5335_08060"
                     /inference="COORDINATES: protein motif:HMM:NF018425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRZ74869.1"
                     /translation="MKVYKSKVDWWLNVLILGVFGYPLVDGIIEKQYVISFVFFGILS
                     ILMWFIHSIKYKIDTEILKIWIYKIEIKTIKRIYKTNIPLSSPAASLDRIAIVYNKYD
                     EIYISPKNREDFIKELLKINSDIIVDV"
     gene            complement(3069..3563)
                     /locus_tag="P5335_08065"
     CDS             complement(3069..3563)
                     /locus_tag="P5335_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRZ74870.1"
                     /translation="MKPPLNELGKLAKDKHIENKKYFDKLKKKQPKNLDYVMQDLHEA
                     EFKRTDCLNCANCCKTTGPLFTSADIERIAKHFKQKPQQFIDQYLQIDEENDYVLKSV
                     PCTFLDAENYCMIYDVRPKACREFPHTDRKKFYQISDLTLKNVAICPAAFTIVEEMKK
                     KLPL"
     gene            3636..4352
                     /locus_tag="P5335_08070"
     CDS             3636..4352
                     /locus_tag="P5335_08070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRZ74871.1"
                     /translation="MKDLFGKAILDFQTGNNPEDLITETTISESDEMSVAYLFRGFEE
                     MPKMEQKALSISKGKILDVGCGAGSHSLYLQEKGFDVTPIDISPNAIKACQLRGLKKA
                     TVQDVMSMKEEQFDTILLLMNGAGMCGRLKNISPFLRQLKSLLTENGQILVDSSDIIY
                     MFDEDEDGGKWIPGDTDYYGELEFKISYKGEMEKPFDWMYIDFNTLQNAASANGLACE
                     LLIEGDHFDYLARLSHQKIT"
     gene            complement(4345..>4574)
                     /locus_tag="P5335_08075"
     CDS             complement(4345..>4574)
                     /locus_tag="P5335_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRZ74872.1"
                     /translation="MKRLDHSKIKSGEKLIVAFTVNELGEMKKIRLVQFKDETTAIEI
                     LNALKNFPKWKPAYKLGNPVSVELKIPIVFK"
     assembly_gap    4575..4674
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4675..4874)
                     /locus_tag="P5335_08080"
     CDS             complement(<4675..4874)
                     /locus_tag="P5335_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74873.1"
                     /translation="MKFIFPVIFILLFSTQSFSQIQGEDEVYLNNLILEPEFPGGMEM
                     FYSYLMKRLDHSKIKSGEKLIVA"
     gene            complement(4877..5509)
                     /locus_tag="P5335_08085"
     CDS             complement(4877..5509)
                     /locus_tag="P5335_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRZ74874.1"
                     /translation="MLQKETQLAVEKGEKLPLMEEFYTIQGEGYHTGTAAYFIRIGGC
                     DVGCHWCDVKESWNAELHPPTSTDLIVSNAKKYADTVVVTGGEPLTWDMSILTKKLRD
                     SGIKIHIETSGCYPVTGFWDWFCLSPKKNKLPVKSAYDIANELKVIIYNKHDFIFAEE
                     QAAQTNPKAILFLQPEWSKREEMTTLIVDYVMKNPKWRVSLQTHKYLNIP"
     gene            5629..>6470
                     /locus_tag="P5335_08090"
     CDS             5629..>6470
                     /locus_tag="P5335_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRZ74875.1"
                     /translation="MSKVEEFSAHINQGYTCKGEFITLGGAILNGEAIPNTHINIPLK
                     TLNRHGLIAGATGTGKTKTIQVLSEQLSKNGIPVLMMDIKGDFSGIAMPGIEQPFITE
                     RHAKINLPYETKAFPVELMTISKQNGVRLRATVSEFGPVLFSRILDLNDTQGGVVSVI
                     FKYCDDHTLPLLDLKDFKKVLQFITEEGKEEIEKEYGRISTSSTGSILRKVIELEQQG
                     ADVFFGELSFDIDDLMRIDENGNGYVNIIRLTDIQDKPKLFSTFMLSLLAEIYNTMPE
                     QGDSG"
     assembly_gap    6471..6570
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6571..6888
                     /locus_tag="P5335_08095"
     CDS             <6571..6888
                     /locus_tag="P5335_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRZ74876.1"
                     /translation="MSRMDVLTTQEIDSHIATSKLAKKYNEVIDRESAFELLTKKINA
                     AQEQASKQAEEANREKEAQKTTTTSTRREPESQTTKAVVKVLTSATFIRGALGILSKL
                     FKK"
     gene            6946..7314
                     /locus_tag="P5335_08100"
     CDS             6946..7314
                     /locus_tag="P5335_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRZ74877.1"
                     /translation="MKGFFTIGLLILSNVFMTLAWYGHLKFKDLKWFENAGLITIVLI
                     SWGLALFEYFFQVPANRIGYEGNGGPFSLLQLKVIQEVITLVIFVVFSMLFFKTETFK
                     WNHFVGMCLLVLAVYFIFRK"
     gene            7341..7703
                     /locus_tag="P5335_08105"
     CDS             7341..7703
                     /locus_tag="P5335_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRZ74878.1"
                     /translation="MKKYYIQKLPFIVPTTDGKLIEEHFGKASDGNNEISIAHMIAPP
                     HWSEPFQTPEFDEYTYIIKGKKQFIIEDEVIVLEAGQSIKIDKNTRVQYSNPFDEVCE
                     YIAICKPAFDFEKVHREE"
     gene            complement(7706..9052)
                     /locus_tag="P5335_08110"
     CDS             complement(7706..9052)
                     /locus_tag="P5335_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRZ74879.1"
                     /translation="MQTPQKIAVVGSGLVGTLLAIYLKKLGHTVHVFDRSADIRTVEF
                     SGRSINLVMSNRGWKALDDIGLTEEIKKIGIPVDKRAIHLQDSKLTYQNYGKEGEAIY
                     SLSRGVLNRKMIDLAEAEGVEFFFEHRIWDITLSDATLHIGETERGEWTDLKYDKVFG
                     ADGAFSRIRHRMQRQSMFDYSQKFLKIGYKELHIPANADGSYKIDKNSLHIWPRGNFM
                     LMGLANLDGSFTCTLFMPFEGENSFESLYDEKTLVDFFAKYFPDTKEVIPDLVEDFFK
                     NPKSYLVTMQCYPWTFNDKVALIGDSCHAIVPFYGHGMNAGFEDITVLSEMISKYGDD
                     WETIFEEYQKSRKPNADAIAELSYRNFMEMSAKTADEKFLLQKKIEKWFSDKHPDLWM
                     PLYSRVTFSLQPYSEALAIGDFQNKIMEEVMQMENIEAIWDSETVEQKIISLLNKK"
BASE COUNT         2963 a         1499 c         1465 g         2831 t
ORIGIN      
        1 aaaaaaatta atcaaatcga atcgctttga caggagaaat ttttgtaata atataagaag
       61 gcaccaacag taccaaaaaa cttataataa tagttccaag gtttaacaaa aagatataaa
      121 accaattaaa ataaatcggt gcttcgctca cataataatt ctccggatta agtttgatga
      181 caccaaattg ttgttgaata agcattaagg caatagctat acaatttccc caaagtaatc
      241 cacggagaat aatatggaag gcattgaaaa gaaatatttt acgaaccatc cagttatcag
      301 ctcctaaagc ttttagaatt cctatcattt gggttctttc caaaatgaga acaagtagtg
      361 ctacaaccat gtttatagtg gccactaata tcataattac taatattaca ataatattaa
      421 agtcgaataa ttgcaaccat tcaaatatgt aaaaatactt gtcaattatg gttttgctgt
      481 ccaacatata tgaggtctgc tcataaactt cttctccttt ttgctctatt ttggtaaaat
      541 catcaataaa tacttcaaaa gctccaactt catcaggttg ccaacggttg attcgttgta
      601 catggcgaat gtctcctaat atatatgtag catcaaattc ctgaaatccg gaattataaa
      661 ttcctgaaat agtaaaaacc cgtaagtttg gcatttcatt actatcttct ttcatgaaaa
      721 aagtattgaa cttatcacca actttaaggt aaagtcgatt cgccaaatac tgagaaatga
      781 gcacttccgg atttaagtct ctagaaacgt tgggtgattt accatcaaca atatactccg
      841 taagattttc ccatttataa tcactgccta ctcctttgaa aataatacct tcaaaagcct
      901 tttctgttcg aattattcca gctttactgg caatagcttg aatatggtta actccctcga
      961 cagaagtaaa cttcgggtaa aaatcctgct tatttgaaac gggtacttta ctgacttctg
     1021 attgattatc gtcaaaatta gaaataatga tatgaccatt aaatgctgaa actttctggc
     1081 gtattttttg ctgtaaacca agaccggtag ccactgaaac tatcatcatg attattccaa
     1141 ttgcaatagc ggtaatagca attttaataa ttggtttaga tatactactt ttatggtctt
     1201 ttgcagtagt aagtcttttg gctataaaat attctaattt caaggtatgt ataataaacg
     1261 gatgttcaaa aatacagttt tattctttgt tttgctaatt gttttgagcg gatgttcagt
     1321 aaagaaacaa gttattgcag ataataataa aaacattgtt atcagtgaag cggtttctac
     1381 tgtggatatc atcacaggag cagaaaattt ttctgcttat ttnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnaagcggtt tctactgtgg atatcatcac aggagcagaa
     1561 aatttttctg cttatttacc actattacaa gacaaaaaag ttggtgtagt tacaaatcaa
     1621 acaggaattg taaactataa aattccttat tctacaacaa tagcagatgg aaaaccacta
     1681 cgccctatta caaaacattt agttgattat ttattggaac acggcataac tgttgaaaaa
     1741 atctatgctc cagaacatgg tttcagagga gttgcagacg caggagagct aattgtagat
     1801 gggaaagatt ctaaaacagg ccttccaatt atatctcttt atggtgataa taaaaaaccc
     1861 aaacctgagc aattggaagg aattgatatt ctactctttg atttacagga tgtcggtgct
     1921 cgattttata cctacatttc atcgctacat tatgttatgg aggcatgtgc ggaaattaat
     1981 cttccactaa ttattttaga tcgaccaaat ccgaacggtg ctattgtaga cggtccaatt
     2041 ttagaaaaag aatttaccag ctttgttgga atgcatccca ttccggtttt acatggcttg
     2101 acaattggtg aatatggtaa aatgattaat ggagaaaaat ggttaaaaga cagtattcaa
     2161 tgcaatttga cagtaattcc ttgtttacat tatcgcaggg atatggacta tagtttacct
     2221 gtaaaaccat cgccaaactt accaaatgac caagcaatta atttgtatgc aagtttgtgt
     2281 ttttttgaag gcactaatgt cagtgttggt cgaggaactg ataaacagtt tcaaatttac
     2341 ggttctcctt tcttacccca aggcgatttc agttttatgc cattaccaaa tttaggttct
     2401 aaaaacccac tccacaacaa tgtggtttgt catggagaag atttgtctga aattagccca
     2461 gtaaagcgac tccggttaaa atggctgatt gaagcttatg aaaacactgc tgataaatca
     2521 actttcttca ctcctttttt tacaaaactg gccggaacaa aactcttgag agtacaaata
     2581 gaacaaggat tatctgaaaa agaaatacga aacacttggg aaaaaggttt agaggagttt
     2641 ttagaaatga gaaaaactta ccttatctat aattagccta aacgtcaaca ataatatctg
     2701 agtttatttt caatagttcc ttgataaaat cttctctgtt tttaggtgaa atataaattt
     2761 catcatattt attataaact attgcgatac gatcaagcga tgctgcggga gaactcaatg
     2821 gaatatttgt tttataaatt cttttaatag tcttaatttc tattttataa atccatattt
     2881 ttaaaatttc agtatctatt ttatatttaa tagaatgtat aaaccacatt aaaatggaaa
     2941 gtattccaaa aaaaacaaat gaaataacat actgcttttc aataatacca tcaactaaag
     3001 gatatccaaa aacacctaga atcaatacat ttaaccacca atccactttt gatttatata
     3061 ctttcatttt acaaaggcaa ttttttcttc atttcttcaa ctatggtaaa agcagccgga
     3121 caaatagcga catttttcaa cgttaagtca gaaatttggt aaaacttttt tcggtcagta
     3181 tgtggaaatt cacgacaggc ctttggacga acatcataaa tcatacaata attttccgca
     3241 tcaagaaaag tacatgggac acttttcaaa acataatcat tttcttcatc gatttgcaaa
     3301 tattggtcaa taaactgctg tggtttttgt ttgaaatgtt tagctattct ttcaatatca
     3361 gccgatgtga ataaaggtcc ggttgtttta caacagtttg cacaatttaa acagtctgtt
     3421 cgtttaaact cggcctcatg caaatcctgc attacataat ctaaattttt aggctgcttc
     3481 ttttttaact tatcaaaata ctttttgttt tcgatatgct tatctttggc aagttttccg
     3541 agttcgttta atggtggttt caagtttaaa gtttaaagtt gagctgcaaa attaatcaac
     3601 atttaacttg aaaccttaaa ctttaaacaa aagaaatgaa agaccttttt ggaaaagcca
     3661 ttttagattt tcaaacggga aataatccgg aagatttaat tacagaaacg actatttcgg
     3721 aatcggatga aatgagtgtt gcctatttgt ttcgaggttt tgaggaaatg ccaaaaatgg
     3781 aacaaaaggc gttatccatt tcaaaaggaa aaattttaga tgtcggttgt ggagccggaa
     3841 gtcattctct atatcttcaa gaaaaaggct ttgatgtaac accaattgac atttcaccca
     3901 atgctattaa agcctgtcag ttacgtgggt taaaaaaagc taccgttcag gatgtaatgt
     3961 caatgaaaga ggaacaattt gacaccattt tattattaat gaatggtgcc ggaatgtgtg
     4021 ggcgattgaa aaacatctcg ccctttttgc gacaattgaa aagcctgcta actgaaaatg
     4081 gtcagatttt agtagacagt tctgatatta tttatatgtt tgatgaagat gaagatggtg
     4141 gcaaatggat tccgggtgat actgattact atggcgaatt agaatttaaa atttcctaca
     4201 aaggtgaaat ggaaaaacct tttgattgga tgtatattga tttcaatacc ttacagaacg
     4261 cagcttcagc aaatggattg gcatgtgaat tactgataga aggagaccat tttgattatt
     4321 tagcccgact aagccatcaa aaaatcactt aaaaacgata ggaattttta attctacact
     4381 taccggatta cctaatttat aagctggttt ccactttggg aagttcttta aggcattcaa
     4441 aatttcaatc gctgtcgttt catctttaaa ttgcaccaat ctgatttttt tcatttctcc
     4501 cagttcattc actgtaaatg cgacaatcaa tttctcacct gatttaattt ttgaatgatc
     4561 caatcgtttc attannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgaca
     4681 atcaatttct cacctgattt aatttttgaa tgatccaatc gtttcattaa atacgagtaa
     4741 aacatttcca ttcctccagg aaattcaggc tctagtatta aattattcaa atatacttcg
     4801 tcttcccctt gaatttgaga gaaactttga gttgaaaaaa gtaaaataaa tataactgga
     4861 aaaataaatt tcaattttaa gggatattaa ggtatttatg tgtttgcaat gaaactcgcc
     4921 atttagggtt tttcataaca tagtccacaa ttagcgttgt catttcttcc cttttgctcc
     4981 attccggctg taaaaaaaga atagcttttg gatttgtttg agccgcttgc tcttcggcaa
     5041 aaataaaatc gtgtttatta taaatgatca ctttcaattc gttggcaata tcataggcac
     5101 ttttaaccgg caatttattt ttttttggag acaaacaaaa ccaatcccaa aaacctgtaa
     5161 cgggataaca accagaggtt tctatgtgaa tttttattcc tgaatctctt agcttctttg
     5221 ttaaaataga catgtcccaa gttaaaggct ctcctcctgt gaccactacc gtatctgcat
     5281 atttttttgc gtttgaaaca attaaatctg ttgatgtagg cggatgaagt tcagcattcc
     5341 aactctcttt aacatcacac caatgacaac ctacatcaca accaccgatt cgaatgaaat
     5401 aagcagccgt tccagtgtga tatccttcgc cttgaatggt gtaaaattct tccataagtg
     5461 gaagtttttc tcctttttca acggctaatt gggtttcttt ttgaagcatt tttaatgaaa
     5521 ttgagtgcaa agatagttaa tttgaaaatg taataatttg aaaatttgaa aatgatagtt
     5581 tcagatttag aattggtttt gttacatttg attattaaaa ctaatacgat gagtaaagta
     5641 gaagaattca gtgcccatat caatcaagga tatacttgta aaggtgaatt tataacctta
     5701 ggaggagcaa ttttaaatgg agaagccatt ccaaatacac atatcaatat tcccttaaaa
     5761 accttaaatc gtcacggatt gattgccgga gcaacaggta cgggaaaaac gaagacgatt
     5821 caggttcttt ccgaacaatt gtcaaaaaac ggtattccgg tgttgatgat ggatattaaa
     5881 ggtgactttt caggaatcgc gatgcccgga attgaacaac cttttattac tgaacggcat
     5941 gctaaaatta atctacctta tgaaaccaaa gcttttccag ttgaattaat gaccatttct
     6001 aaacaaaatg gggtaaggtt acgggcaacg gtttcagaat ttggacccgt tttattttca
     6061 agaatattag atttaaatga cactcaaggt ggagttgttt ctgtaatttt taaatattgt
     6121 gacgatcata cattaccatt attagattta aaagatttca aaaaagtttt acaatttatc
     6181 accgaagagg gaaaagaaga aattgaaaaa gaatatggaa gaatttccac ttcttcaacc
     6241 ggttcaattt tgagaaaagt gattgaattg gaacaacaag gagctgatgt tttttttggg
     6301 gaattatctt ttgatatcga tgatttgatg cgaatagacg aaaacggaaa cggatatgtg
     6361 aacataatcc gattgaccga cattcaagac aaaccaaaac tattttcaac ttttatgttg
     6421 agtttattgg cagaaatata caatactatg cctgaacaag gcgattccgg nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atgagcagaa tggatgtctt aactacacaa
     6601 gaaatcgatt cgcatattgc tacatcaaaa ttagctaaaa agtataacga agttattgac
     6661 agagaaagtg ctttcgaact tttgacaaaa aaaattaatg ccgcacaaga acaagcttca
     6721 aagcaagccg aagaggcaaa tagagaaaaa gaagctcaaa aaacaactac tacctcaact
     6781 cgtcgtgaac cggaaagtca aactacaaaa gcagttgtaa aagtactgac aagtgctact
     6841 tttattcggg gagctttggg aattttgagt aaactgttta aaaagtaaaa acctctcccc
     6901 ctagcccctc tccaaaggaa aggggagcaa gaaacgaact aaaaaatgaa aggattcttc
     6961 accatcggat tacttatact ttccaatgtt tttatgacat tagcatggta tggtcatttg
     7021 aaatttaaag atttgaaatg gtttgaaaat gccggattga ttacgattgt attaatcagt
     7081 tggggattgg cgttgtttga atactttttt caagtaccgg caaaccgaat tggttatgaa
     7141 ggtaatggcg gtccattttc acttttacaa ttaaaagtga ttcaagaagt gattacacta
     7201 gtcatttttg tggttttttc gatgcttttc tttaaaactg aaacctttaa atggaatcat
     7261 tttgttggga tgtgcctttt ggtgttggcg gtttatttta tatttagaaa atagagaaaa
     7321 gaacaaagag aatagacatt atgaaaaaat attatattca aaaattgcca ttcatcgttc
     7381 caacaaccga tggaaaacta atcgaagaac atttcggaaa agcttctgat ggcaacaacg
     7441 aaatttccat tgctcatatg attgcaccgc cgcattggag tgagccgttt caaacaccag
     7501 aatttgacga atatacatat atcattaaag gtaaaaaaca atttataatt gaagacgaag
     7561 taattgtttt agaagctggt caatccatca aaattgataa aaacactcgc gttcaatatt
     7621 ccaatccgtt tgatgaagta tgtgaatata ttgctatttg caaacctgct tttgattttg
     7681 agaaggtaca tcgggaagag taaaactatt ttttgttcaa caacgaaata atcttctgtt
     7741 caaccgtttc actatcccaa attgcctcaa tattctccat ttgcatcact tcttccatga
     7801 ttttgttttg aaaatcacca atggctaaag cttcggaata gggttgcaaa ctaaatgtta
     7861 ccctactata caacggcatc cataaatcag gatgtttgtc tgaaaaccat ttttcaattt
     7921 tcttttgcaa taagaatttc tcatcggcag ttttggcact catttccatg aaattgcgat
     7981 aggaaagttc ggcaatggca tcggcattgg gtttacgtga tttttgatat tcttcaaaaa
     8041 tggtttccca atcgtcaccg tattttgaaa tcatttcact taaaacggta atatcctcaa
     8101 aaccagcgtt cattccgtgg ccgtaaaatg gaacaatcgc atgacaggaa tcgccaatca
     8161 acgcaacttt atcgttaaat gtccaaggat aacattgcat ggtaaccaaa taacttttag
     8221 ggtttttgaa gaaatcttcc accaaatctg gaatcacttc tttggtatca ggaaaatatt
     8281 tagcaaaaaa atcaaccaac gttttttcgt catataaact ttcaaaggaa ttttctcctt
     8341 caaaaggcat gaaaagcgta caagtaaacg agccatctaa attagccaat cccatcaaca
     8401 tgaaattgcc gcgaggccaa atatgtaaag agtttttatc aattttataa cttccgtctg
     8461 cattagcagg aatgtgcaat tctttgtagc caatttttaa aaatttttgc gaataatcaa
     8521 acatactttg gcgttgcata cggtgacgaa ttctcgaaaa cgcaccatcg gcaccaaaaa
     8581 ctttatcata tttcaaatcc gtccactcgc ctctttcggt ttcgccgatg tgcaaagtgg
     8641 catcggataa tgtaatatcc caaattctgt gttcaaagaa gaattcgact ccttcggctt
     8701 cagctaaatc aatcattttt cggttcaaaa cacctctgga aagtgaataa atggcttcac
     8761 cctctttacc gtaattttga taagtaagtt ttgaatcttg caaatgaatc gcccgtttat
     8821 cgaccggaat tccgattttc ttaatttctt cggttaaacc aatgtcatcc aacgctttcc
     8881 aacctcgatt tgacatgacc aaattaatgg aacgtccgga aaattcaact gttcgaatat
     8941 ccgcacttcg atcaaaaaca tggaccgtgt gacctaattt tttgaggtat attgccaata
     9001 aagttccaac taatccggaa ccaacaacag caatcttttg aggggtttgc ataaatga
//
LOCUS       DAOTCW010000091         9003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_5316_length_9003_cov_40.850246, whole genome shotgun sequence.
ACCESSION   DAOTCW010000091 DAOTCW010000000
VERSION     DAOTCW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9003
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5316_length_9003_cov_40.850246"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="P5335_08115"
     CDS             <1..1416
                     /locus_tag="P5335_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRZ74880.1"
                     /translation="ITVFNLANGNYSINYSLTGSNILANQTVLLTVTDGEGSFTIDST
                     QLVNSGITTLILNSITNSVTTCTTVLSGLNFAIEVLPIPSISNSTIQIVNVCFGNDVL
                     VQISGATNLTDGNYQFVYSIPNANPIIGNSGIISIVSGNGQFAITATVFPLAGVYTMS
                     LISISNLATNCTNLNPNVAVTFEILAQPTITEALVSMAGICVNTEGTVLITNANNLSD
                     GTYQLTYQISGAINFNETIPVVFTNGETSFVIPSSVTNNFGNITISINPINSNTGNAC
                     GFSGHTFNTVTIAIEEVETPQLIEGGNSFCEDDNSTIADLTNGIIGSETIIWYDAPSS
                     GNAYSNSDLLVDGVTYYASIQSASGCESAVRLQVTVVIKDCTDIIIPDGFSPNGDGIN
                     EIFVINNIRTLYPNFTLEIFNRYGNILYKGNANSLDWDGTSDKGIQIGGNKLPVGVYF
                     YVINFNDGSRKPLQGRVYLSR"
     gene            1431..2375
                     /locus_tag="P5335_08120"
     CDS             1431..2375
                     /locus_tag="P5335_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRZ74881.1"
                     /translation="MIEKHIKYKIHLIILLVTLVSNAQQDPQYTQYMYNMSVINPGYA
                     TDDAEVINFGALYRAQWVGSEGGPTTGTFFAHSPIAKKVEMGISVVHDEIGDVVKESN
                     VFVDFAYVLNLNESNKLSFGIKGGATFYSTNFNGFIYSDELPDPAFAENLSKTFPNIG
                     VGAFYFGENYYLGLSSPNLLKSKHLEKQDGVIATGVEEIHYFFTGGYVFNLNDNLKFK
                     PAFMTKAVAGAPMSLDLTANFLINNVFELGAGYRWDDSVSGLVNFKITPSLRIGYAYD
                     YTLSNLGKFNTGSHEIMLLFDLNKKGKTNGYDKSPRFF"
     gene            2386..4257
                     /locus_tag="P5335_08125"
     CDS             2386..4257
                     /locus_tag="P5335_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRZ74882.1"
                     /translation="MRKNFYILFFLLVCQLMLAQKPTVKKANELFANKAYVEAAKMYE
                     QLKQDQKVLQNLADSYYYNAQMKFASTHYGTLFFTYKDSVKPEYYFRYANALKGIDDY
                     EKGDVIMGEYLKYSVDTKKFKEHLNNIVPFNYTVRQMSRSNSTGDFGISFFGEKVVFA
                     SLRNTESPLYKWNDRPYLDLYEATVSKDGILENIKPFSEIINTKTHESSATFSKDGKT
                     MYFNRTNDVQVQVGEEKFASVKLFKAVFENNAWTNVTELPFSSDQFSTQHPMLSPDEK
                     KLYFSSDRPGSIGSFDIYFVDIFEDGGYGEPQNLGDNINTIHREQFPFIAEDETLYFA
                     SEGLQGLGGLDIFMARTYDGVYGKPINLGETINTGMDDFAYVLKANEDIGYFASNRKG
                     TDNLYSFVREENERRFIVEGDVRDINTKELLPGTTVTLYDMDDKLIGQLVVGENADYV
                     FNSEPNTKYKIVADRDFYASKTEIFETTDDGRVRFTIELEIESYDDAEDIIVTKEDGL
                     VYIQLENIYFDLDKWDIKPEAANTLDVLVAIMKKYPRMEIELGAHTDTRASDAYNLRL
                     SHNRANSTLEYLVENGIERNRMRSKGFGERVPLINCGDNCSEVEHSINRRVEFIVRK"
     gene            complement(4259..4831)
                     /locus_tag="P5335_08130"
     CDS             complement(4259..4831)
                     /locus_tag="P5335_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRZ74883.1"
                     /translation="MASDYIFNEEIEHIVFEESGIRYKEYENCIFKKCDFSACDFSGV
                     VFVECTFYECNFKETKINYVSLRDVQFVGCNFTAVNFAMTDQVIYNFNFTDCLLDYAQ
                     FYALKLKKMQFINCSMVSVDFMGSDLTEVLFDHCNLRRAVFIDTNLEKADFYSSYDFV
                     LDPEKNKLKKAIFSTEGLKGLLSKYDLVIK"
     gene            complement(4894..4967)
                     /locus_tag="P5335_08135"
     tRNA            complement(4894..4967)
                     /locus_tag="P5335_08135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4931..4933),aa:Arg,seq:acg)
     gene            5036..5548
                     /locus_tag="P5335_08140"
     CDS             5036..5548
                     /locus_tag="P5335_08140"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRZ74884.1"
                     /translation="MNLEKYIRIIQDFPKPGIIFKDITPLLITPSARQECLSILLENV
                     KELKIDKVIGVESRGFFFGILLANELNVGFIPVRKPNKLPFDTISASYDLEYGTDALE
                     IHTDAIQKGDRVLIHDDVLATGGTAKAVCELVERLGGEIVQVNFLMELTFLDGREKIK
                     QYPISAALTY"
     gene            complement(5545..>5661)
                     /locus_tag="P5335_08145"
     CDS             complement(5545..>5661)
                     /locus_tag="P5335_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HRZ74885.1"
                     /translation="LLPSFTKQGLDLAKAAKWQMAIIGWRYYVTTRALDETP"
     assembly_gap    5662..5761
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5778..6989)
                     /locus_tag="P5335_08150"
     CDS             complement(5778..6989)
                     /locus_tag="P5335_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRZ74886.1"
                     /translation="MLKTAFLRYINNFRGFTREVWILAIITFINRAGTMVLPFLSKYL
                     KEDLAFSYREVGWIMVCFGAGSMLGSWIGGKLADKIGFYKVMIFSLMSTGFLLFIFPY
                     ITEFYTLCIAWFFVMIIADMFRPAMFVSLGAYAKPENRTRALTLVRLAVNLGFAAGPA
                     LGGLIIMNMGYSGLFWVDGATCILAISIFWILVKEKKKTTADAHDAFEMPKRSVYQDK
                     PFWVFLFVSFVTAMIFFQLFTTLPLYHKEMYGLTEFQTGLLMTLNGFLIFLLEMPIVG
                     FFERKQINRLKIVLFGSFCMALGFFSLLINVWAGILVINILFLTFGEMFAFPFSNSFA
                     LSRAPRGQEGRYMALYTMSFSLAHIMSSKMGMELIAVFGYQMNWLVMACFGLLAAGCA
                     LWVMKMVREET"
     gene            7385..8008
                     /locus_tag="P5335_08155"
     CDS             7385..8008
                     /locus_tag="P5335_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ74887.1"
                     /translation="MKTKLLFLMFFGQIFISNGQSIQINQITPTAVSDGINVNLLVTT
                     YNGAGYLSHSYTVIENTINLSVCYWFNTTLPIYQINNDFLISVPNNINYIINVSIFNS
                     FSQTVCDNFSTGPTASTNYLEIENFKEANNKYLIYPNPSKGTIELIGDESLIKHIKIY
                     DNLGRLIKQLNERITKNIDLYELNDGIYIINIETESGNLNQRFIIKK"
     gene            8179..8997
                     /locus_tag="P5335_08160"
     CDS             8179..8997
                     /locus_tag="P5335_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRZ74888.1"
                     /translation="MKLIYYYSLFILVTLTSCSKAAQPNDPIPQHETLKIQSKHVGEE
                     RIINVWTPSNYKTSSDSLPVMYMADGGIKEDFPHIANTIAKLIKENKIKPLILVGIEN
                     TQRRRDLSGFTEVAKDKEIAPVVGGSEKFRAFIKDELFPEINKRYRTTSEKSIIGESL
                     SGLFVVETFLLTPNMFDNYIAFDPSLWWNNHYLVRTAKEQLSKFPTTEKRIWFASSKA
                     EDISPYTKELATILKTENKANLKWKFSDEPKEKHNTIFRATKEKAIIWTLNKTE"
BASE COUNT         3229 a         1580 c         1463 g         2631 t
ORIGIN      
        1 attacagtat tcaatttggc gaatggtaat tattcaataa attattcatt aacaggtagt
       61 aatattttag caaatcaaac agttttatta actgttacag atggtgaagg aagtttcaca
      121 atagattcaa cacaattagt taattcagga attactactt taattttaaa ttctattaca
      181 aattctgtta ctacttgtac aacagtatta tccggattaa attttgctat tgaagtttta
      241 ccaataccaa gtatttcaaa tagtactatt caaattgtaa atgtttgttt tggtaatgat
      301 gtacttgttc aaatttccgg agcaaccaat ttaacagatg gaaattacca atttgtatat
      361 tctattccta acgcaaatcc aataattgga aattcaggca ttatttcaat agtgagcggt
      421 aatggacaat ttgctataac tgcaacagtt tttccattag caggtgttta tactatgtca
      481 ctaatttcaa tttcaaattt agcaacaaat tgtactaatt taaatccaaa tgtcgcagtt
      541 acatttgaaa tattagccca gccgacaatc acggaagcat tagtatcaat ggccggaata
      601 tgtgttaata cagaaggaac agttttaatt acaaatgcaa acaatttgtc agatggaacc
      661 tatcagttaa cctatcaaat ttccggagca ataaatttta atgaaacaat cccggttgtt
      721 tttacaaatg gggaaacaag cttcgttatc ccttcttcag taacaaataa ttttggaaat
      781 attacaattt caattaaccc tataaattca aatacaggca atgcatgtgg tttttccggg
      841 cataccttta atacagtaac cattgctatt gaagaagttg aaactccaca attaatagag
      901 ggaggaaatt cgttttgtga agatgataat tctacaattg ccgatttaac aaatggtata
      961 attggaagcg aaactataat ttggtatgat gctccatcaa gcggtaatgc ttacagtaac
     1021 tcagatttat tggtagatgg agtaacttat tatgcatcca ttcaatctgc ttccggatgt
     1081 gaaagtgcag taagacttca ggtaaccgta gtgattaaag attgtacaga tataatcatc
     1141 cctgatggtt tttctccaaa tggtgatggt ataaatgaaa tttttgtaat caataatatt
     1201 cgaaccctct atccaaattt tacgctcgaa atattcaatc gctatggaaa tattctttac
     1261 aaaggaaatg caaattcgct cgattgggat ggaacttctg ataaaggaat tcaaataggt
     1321 gggaataaac ttcccgtggg agtatatttc tatgttataa actttaatga tggcagtaga
     1381 aaaccattac aaggtagagt ttatttaagc agataaaaga ttaaaacgtt atgatagaaa
     1441 aacatataaa atataaaatc catttaatca tcttattggt gaccttggtt tcaaatgccc
     1501 aacaagaccc tcaatatacg cagtatatgt ataatatgag tgttattaat ccgggttatg
     1561 caacagatga tgcagaggtc attaattttg gtgccttata tagagctcaa tgggttggtt
     1621 cagagggagg cccaactacc ggaacttttt ttgctcattc tccaattgct aaaaaagtag
     1681 aaatgggaat ctctgtggtt catgatgaaa taggggatgt agtaaaagaa agtaatgtat
     1741 ttgttgattt tgcttatgtt ttaaacttga atgaatctaa taaactttct tttggaatta
     1801 aaggaggagc aactttttat agcacgaact ttaatggttt tatatattca gatgaattac
     1861 cggatcctgc atttgctgaa aatttgagta aaacttttcc aaatatcgga gttggagcat
     1921 tttattttgg agagaattat tatttaggat tgtcaagtcc aaatctttta aaatcaaagc
     1981 atcttgaaaa gcaggatggt gttattgcaa ctggagttga ggaaatacat tattttttca
     2041 ctggtggata tgtttttaat ttaaatgata atttaaaatt taaaccggct tttatgacta
     2101 aagcggttgc aggtgctcca atgtcattag atctcacagc caatttttta atcaataatg
     2161 tttttgaact aggtgccggt tatcgttggg acgactccgt gagtggttta gtaaatttca
     2221 aaattacacc ttcgttacga attggatatg cttatgacta caccctttcc aatttaggaa
     2281 aattcaatac cggaagccat gaaattatgt tattatttga tttaaataaa aaagggaaaa
     2341 caaacgggta tgacaaatcg ccacgatttt tctaatataa taaagatgag aaagaacttt
     2401 tatatactat tctttttgtt ggtttgccaa ttgatgttgg ctcaaaagcc tacggttaaa
     2461 aaagcaaacg aactttttgc caacaaagct tatgtcgaag ctgcaaaaat gtatgaacag
     2521 ctcaaacaag atcaaaaagt tttacaaaat ttagctgatt cttattacta caatgctcaa
     2581 atgaaatttg catcaacgca ttacggaact ttatttttta cttacaaaga cagtgtaaaa
     2641 cctgaatatt attttagata tgccaacgcg ttgaaaggaa ttgatgacta cgaaaaagga
     2701 gatgtaatca tgggcgaata tttaaaatat tcagttgata ccaagaaatt taaagagcat
     2761 ttaaataata tcgtaccttt taattacacc gtacgtcaaa tgagccgaag caatagtact
     2821 ggtgattttg gaatttcttt ttttggagaa aaagtagttt ttgcttcact cagaaacaca
     2881 gaaagtccac tttataaatg gaacgacaga ccatatctcg atttatatga agcaaccgtt
     2941 tctaaagatg gtatattaga aaacatcaaa cctttttctg aaatcatcaa cacaaaaaca
     3001 catgaaagtt cagcaacctt ttccaaagat ggcaagacga tgtattttaa tcgtaccaat
     3061 gatgtacaag ttcaagttgg ggaagaaaaa tttgcttctg taaaactctt caaagccgta
     3121 ttcgaaaata atgcttggac aaatgtaacc gagttgcctt tttcaagcga tcagttttca
     3181 acacagcatc cgatgttaag tcctgatgaa aagaaattat atttctcaag tgatcgtccc
     3241 ggttctatag gctcgtttga tatttatttt gttgacattt ttgaagacgg aggttatggc
     3301 gaacctcaaa acttaggtga taatatcaac accattcatc gtgaacaatt tccttttatt
     3361 gcggaagacg aaacacttta tttcgcctct gaaggtcttc aaggtttggg cggattagat
     3421 atttttatgg ctcgaacgta tgatggcgtc tacggaaaac caatcaattt aggagaaacc
     3481 atcaacaccg gaatggatga ttttgcctat gtgttaaaag ccaacgaaga cattgggtat
     3541 tttgcctcta accgcaaagg aacagataat ttatattcct tcgttcgcga agaaaacgaa
     3601 cgtaggttca tcgtggaagg tgatgttcgc gacataaaca ccaaagaatt gctacccgga
     3661 acgaccgtca ccttgtatga catggatgat aagttaatcg gtcagctcgt agtaggtgaa
     3721 aatgcagatt atgtattcaa ttctgaaccc aatacaaaat ataaaattgt agctgatcgt
     3781 gatttctatg cttctaaaac tgaaattttt gaaacgacgg atgatggtcg ggttcgattc
     3841 acaatcgagt tagaaattga atcctatgat gatgcagaag atatcattgt aacgaaagaa
     3901 gatggtttag tctacattca attagaaaac atttattttg atttagataa atgggatata
     3961 aaaccggaag cagcaaacac gttggatgtt ttagtggcca ttatgaaaaa atacccacga
     4021 atggaaattg agttgggtgc ccacaccgat acccgagcat cggatgccta taatttacgc
     4081 ttgtctcaca acagagctaa ttcaacctta gagtacctag tagaaaatgg aatcgaaaga
     4141 aacagaatgc ggtcaaaagg ttttggtgaa cgagttcctt taattaactg tggcgacaat
     4201 tgttctgaag tcgaacattc aatcaaccgt agagtagaat tcattgtacg gaaataaatt
     4261 atttgataac taaatcatat ttagaaagca atccttttaa accttcagtt gaaaagattg
     4321 cttttttaag tttatttttt tctggatcta aaacaaagtc ataactgctg taaaaatctg
     4381 ctttttctaa attggtatca ataaatacgg ctcgtcttaa attgcaatgg tcaaataaaa
     4441 cctcggttaa atcgcttccc atgaaatcaa cggaaaccat actacaatta atgaactgca
     4501 ttttttttaa tttcaaggca taaaactgag catagtctaa caagcaatcc gtaaaattaa
     4561 aattataaat cacctgatcg gtcatcgcaa aattaacggc cgtaaaattg catcccacaa
     4621 actgaacatc gcgtaacgaa acataattaa tcttcgtttc cttaaaattg cattcataaa
     4681 aagtacattc tacaaataca acgcccgaaa aatcacaagc tgaaaaatca cattttttaa
     4741 aaatgcaatt ttcatattct ttataccgaa taccactttc ttcaaaaaca atatgttcta
     4801 tttcttcgtt aaatatataa tcagaagcca ttgcaaaagg atataaaaag ttataaaaaa
     4861 agcttctcga aggggagaag cttttaattt ttgtgacctc gactggattc aaaccagtaa
     4921 ccttctgagc cgtaatcaga tgcgctattc agttgcgcca cgaggcctta atgcgggtgc
     4981 aaatatagga atatttgtgt aacttgcaaa ccaaatttaa aaattactgc tttttatgaa
     5041 ccttgaaaag tacatccgca tcatacaaga tttccctaaa ccgggaatca tctttaaaga
     5101 cattacacca ctattaatca caccgtccgc tcgtcaggag tgtttgtcta ttctattgga
     5161 aaacgtaaaa gagttaaaaa tcgacaaagt aatcggtgtc gaaagtcgtg gctttttctt
     5221 cggaatatta ttggcaaacg aactgaatgt tggcttcatt ccggttagaa aacctaacaa
     5281 attgcctttc gataccattt ccgcctcata cgatttagaa tatggaacag atgcattgga
     5341 aatccatacc gatgccattc aaaaaggcga ccgcgtcctc atccacgacg atgtgttagc
     5401 caccggaggc actgcaaaag ccgtgtgcga attagtagaa cgcctaggcg gtgaaatcgt
     5461 tcaagtcaac tttctaatgg aattaacctt cctggacgga agagaaaaaa ttaaacaata
     5521 ccctatttcc gcagcattaa cgtattaggg agtttcgtcc aacgcccgcg ttgttacata
     5581 atatcgccaa ccgataatcg ccatctgcca tttggcagct ttggctaaat ccaaaccttg
     5641 tttagtaaaa ctaggtaata gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 ntaaaattag tcaaaaatta cgtttcttcc cgcaccattt tcattaccca aagggcacaa
     5821 ccggctgcga gtaaaccaaa acaagccatc accagccaat tcatctgata gccaaacaca
     5881 gcaattagtt ccatacccat ttttgaactc ataatgtggg ccaaactaaa actcatcgta
     5941 tacaacgcca tatagcgtcc ttcctgaccg cggggagctc tgctcaaggc aaaagaattt
     6001 gaaaaaggaa aggcaaacat ctccccaaaa gtcaaaaaca gaatattaat gaccaaaatt
     6061 cctgcccaca cattaatcaa taacgaaaag aatcccaagg ccatacaaaa cgaaccaaaa
     6121 agaacaatct ttaaccgatt aatttgcttg cgttcaaaaa atcccacaat aggcatttct
     6181 aataaaaaga ttaaaaatcc gttcaacgtc atcaacaaac cggtttgaaa ttcagttaag
     6241 ccatacattt ccttatgata caacggaagg gtagtaaaca actgaaagaa aatcatcgcc
     6301 gtaacaaaac tcacaaataa gaaaacccaa aaaggtttgt cctgataaac cgaacgttta
     6361 ggcatttcaa aagcatcgtg tgcgtctgcc gttgtttttt tcttctcttt caccaatatc
     6421 caaaagatag agatggccaa gatacaagta gcaccatcca cccaaaacaa cccggaatac
     6481 cccatgttca taataatcaa tccccccaag gcaggccctg cagcaaatcc tagattaaca
     6541 gccagtcgta ccaaggttaa ggcacgggtt ctgttttccg gttttgcata agcacctagc
     6601 gaaacaaaca tcgccgggcg aaacatatcg gcaataatca tcacaaaaaa ccaagcaata
     6661 caaagtgtat aaaactccgt gatgtatgga aatataaaca acaaaaatcc ggtcgacatc
     6721 aaactgaaaa tcatcacctt ataaaacccg attttgtccg ccagtttccc tccaatccaa
     6781 gaacccagca tcgaaccggc tccaaaacaa accataatcc aacccacttc gcggtaactg
     6841 aatgccaaat cctcttttaa atatttagat aaaaacggca aaaccatcgt tcctgctcga
     6901 ttgataaaag tgataatagc taaaatccaa acctctctcg taaagccacg aaaattatta
     6961 atatacctta aaaaagcagt tttgagcatg ttggatgttt caagcggtaa aggtaaaaaa
     7021 ctttaaaggc ctaaaactta ctccgcaatt attttttaaa cgagttgata acaaccaaac
     7081 taatccccaa aaccacttct cctcgctcta cgaagtctga cttcgtagta atcttataaa
     7141 ccaaagaacc aaaaaataaa aagaacaaaa aacaaaacat tctcttccta caataaccac
     7201 atctacaaaa ctgatgacct gaaattccct gtcccgaagc ttcgggagga tagatggtta
     7261 aatacactta ttttctgttt tcttacatgt ttttaagtat atttgttaaa gagattgtgg
     7321 aggtttttca ctgtctgagg ttgtgccgtt atattaaaaa aatagaatta aaaaaatata
     7381 tattatgaaa acaaaattac tttttttaat gttttttgga cagattttca tatctaatgg
     7441 acaatcgata caaatcaatc aaataactcc aacggctgtt agtgatggta taaacgtaaa
     7501 tttactagtg acaacttata atggtgccgg atatttgagt catagttata cagtgattga
     7561 aaatactata aacctatcag tttgttattg gtttaatact actttaccaa tttatcaaat
     7621 taataatgac tttctgatta gtgttcccaa caatataaat tacattataa atgtcagtat
     7681 ttttaattca ttttcccaaa cagtatgtga taatttttct actggtccaa ctgcttcaac
     7741 taattatttg gaaatagaaa attttaaaga agcaaacaat aaatatttaa tttatccaaa
     7801 tccaagtaaa ggaacaatcg aattaatagg agatgaatct ttaataaagc atataaaaat
     7861 ttatgataat cttggtagat taataaaaca actaaatgaa agaataacaa aaaatataga
     7921 tttatacgag ttaaacgatg gcatttatat tattaatatt gaaacggaaa gcggaaattt
     7981 gaatcagaga tttataataa aaaaataagt acagccacta acagtggtag ctgttgcaca
     8041 acctcgttta gaaagatcta aacaaaagtt tttttgtaac atactgaaaa accatttctg
     8101 cctaccccga aactgccgac cgaccttttg acaacagtaa acgaagtaga aaattagtac
     8161 caacattaaa caaaaaacat gaaattgatt tattactact cactattcat actagtaaca
     8221 ttaacaagct gttcaaaagc agcacaacct aacgacccaa taccacaaca cgaaacactt
     8281 aaaattcaat ctaaacatgt tggagaagaa agaataatta acgtttggac tccctcaaac
     8341 tacaaaacaa gttcagactc attgccggtt atgtatatgg ctgatggtgg aattaaagaa
     8401 gacttccctc acattgctaa tacgatagct aaactaatta aggagaacaa aataaaacct
     8461 ttaattcttg taggaataga aaatacacaa cgaagaagag acttgtcagg ctttacagaa
     8521 gttgcaaaag acaaagaaat tgcaccggtt gtcggaggtt cagaaaaatt tagagcattt
     8581 attaaagacg aactttttcc cgaaatcaat aaaagatata gaacaacttc tgaaaaaagt
     8641 attattggcg aatcattgtc aggtcttttt gtggtggaaa ctttcctttt aacgcctaat
     8701 atgtttgaca attatatagc ctttgaccct tcactgtggt ggaataatca ctatttagta
     8761 agaacagcca aagaacagtt atctaaattc ccaacaaccg aaaaacgaat ttggtttgca
     8821 agttcaaaag cagaagatat ttcgccttat accaaagaat tagcaactat tttaaaaaca
     8881 gaaaacaaag caaacttaaa atggaaattt tcagacgaac ctaaagaaaa gcacaacacc
     8941 atttttagag caacaaaaga aaaagcaatc atttggacat taaacaaaac ggaataaaaa
     9001 acc
//
LOCUS       DAOTCW010000092         8872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_5423_length_8872_cov_37.659068, whole genome shotgun sequence.
ACCESSION   DAOTCW010000092 DAOTCW010000000
VERSION     DAOTCW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5423_length_8872_cov_37.659068"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..2120)
                     /locus_tag="P5335_08165"
     CDS             complement(93..2120)
                     /locus_tag="P5335_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74889.1"
                     /translation="MTTNTVLLLLLSVLLAGGVSFFQYFYKAKSNSKINWILAFLRFC
                     SIFGLLLLLINPIITRKSFETIKIPLPVVVDNSSSVAEFKANEIALEVYQNIVSNAEL
                     KEKFNIQSYQFSAELEASETFDFKGTQTRIDGVGKGLKSIHKSINHPTVIITDGNQTY
                     GNDYIYSFDKNKSVYPIVLGDTTAVFDLKISQLNVNKYAFLKNKFPVEVFVNYSGKKN
                     VNATFSISQGNTILNKQNINFTQNQKSHLITVLLPADKVGVQIFKAAISSPEIEKNKY
                     NNSKNFAVEVIDQKSEIAIISTINHPDIGALKRAIETNQQRKVTIVKPNQINSLQDFN
                     VLILYQPTSEFKTVFEKNVVANLNTWIITGTKTDFTFLNQQQDAFSFKMSSQQEDYLA
                     TFSSQFNLFASDNIGFENFPPLQNAFGTITPKIKMETLLTARILNVELNTPLLSYVDI
                     QGKRTCFLFGENSWKWRLQSHIDQQSFEKFDVFIDKTIQFLATNNARKSLVVTHENFY
                     NSGDAIEINTQYFNKNYEFDDKARLSISVKNKTTNQTKSYDLLKSTNSFKVNLDGLPA
                     GNYSFTVTENNSKTSHAGFFEVLDFDIEKQFVNPDYVKLNQLATQTNGKTFLANQVEE
                     LVKSLISNDNYKPIQKEIVKKTPIIDWIWLLIIVVAALVSEWFIRKYNGLL"
     gene            complement(2179..2550)
                     /locus_tag="P5335_08170"
     CDS             complement(2179..2550)
                     /locus_tag="P5335_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRZ74890.1"
                     /translation="MRIFSFEKLIAWQKGRELALLIYKTTKKFPKEETFGLSSQMRRC
                     SISITSNLAEGSRRKSFKDKARFSEIAYSSSLELLNQVILSNDFEYIDEINYLSLREK
                     IAEVNMLIDGLYISQIKNLEH"
     gene            complement(2595..3341)
                     /gene="fabG"
                     /locus_tag="P5335_08175"
     CDS             complement(2595..3341)
                     /gene="fabG"
                     /locus_tag="P5335_08175"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRZ74891.1"
                     /translation="MKLLEGKVAIITGASRGIGSGIAKVFAQQGANVAFTYSSSAESA
                     LVLENELNAIGIKAKGYKSNAADFNEAQKLADEVMADFGNIDILINNAGITKDNLLMR
                     MSEEDFDKVIEINLKSVFNMTKAVQKIMLKNRKGSIINMSSVVGVKGNAGQANYAASK
                     AGMIGFTKSIALELGSRNIRCNAIAPGFIETEMTGKLNEEVVQGWRDSIPLKRGGTPE
                     DVANACLFLASDLSAYVTGQVMNVDGGMLT"
     assembly_gap    3417..3516
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3517..5400
                     /locus_tag="P5335_08180"
     CDS             <3517..5400
                     /locus_tag="P5335_08180"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRZ74892.1"
                     /translation="ILGQYMHAPLANYANVYINGNLIGLYTNVESISKKFVNTHFGSN
                     NNAFFSCSPPAGAGPGTTNLPNLAYLGTNSASYESAYEIKSTTGWDDLINLTNTLSNN
                     TSNIESVLDVDRALWMLAFDNLFVNIDSYIGQFKQNYYLYKDDNGRFNPILWDLNMSF
                     GTFGQTGTTNLNTTLAKRQLNHLLHSGDAAWPLVQKLLAVPRYKKMYLAHYATMLSEN
                     ISNNSYFTKAQTFQTLINSSVQADVNKFYTYAQFSSNMTTDIALGMNTAPGLSNLMSG
                     RNTYLTALSDFTATKPSISAITPSVANPALGGSVTIRANVTNTNTDAVFLGYRAASNL
                     PFTKVLMFDDGAHNDGASGDNIYGGLMPISSITSQYYIYAENNTIGAFSPARAEHEFY
                     TITASYPTITPGSLVVNEIMAQNTSTVTDPSGDYSDWFELYNNSSVTISLDNLFASDT
                     STNLLKWQFPSGITMAPNSYLIVWADQDLTQEGIHADFKFSASGESCILSYANGIIIE
                     NVTFGVQVANMGYARVPNGTGNFVIQAPTFNANNQGLSISDFNQQNNQLLIYPNPTSN
                     YVNIRISELMGSEKIKIFNLLGQIVIEKEAHNDNTLDLTILSAGTYVVNYGKINKKIV
                     VFK"
     gene            5411..5800
                     /locus_tag="P5335_08185"
     CDS             5411..5800
                     /locus_tag="P5335_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74893.1"
                     /translation="MKKLLLLVLIVTSFTFSSCSKEEGYGGLATIKGKVFGKDVNSNG
                     NLVGEGYVGGFKVYISKHGNSASFDDVDSSYDGTFQFEFLYEGTYDIWVFGDCDSCAW
                     DQVYVLRTVEITSKKQTVEVEDLVITF"
     gene            5807..6559
                     /locus_tag="P5335_08190"
     CDS             5807..6559
                     /locus_tag="P5335_08190"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRZ74894.1"
                     /translation="MFNKFLSNLQQLNPTTLSELDSVSLQNRIDSKYILNEKEMVNLL
                     PCLIENYKILTIKDERVFSYENNYFDTSDYLFFKDHHNGYANRIKVRSRRYVESDLCF
                     FEIKKKEKVNRTNKHRESLVSLVSDINEEKRELIQSFTRKELIDLQLILKNNFKRITL
                     VNNEFTERVTIDFKIHFADETNEVNFEGITVVEIKQSKSSQASPLVSFLKESNTREQS
                     FSKYIFGVISLKPEIKKNNFLPILKNINKIKN"
     gene            6559..7200
                     /locus_tag="P5335_08195"
     CDS             6559..7200
                     /locus_tag="P5335_08195"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRZ74895.1"
                     /translation="MEEAVNTVKNAFDATDFGIRAAIDFIAVVVLVSLIYYPKHKNKD
                     FIFTFVLFNVINFMICYLLGSAKIKIGFAFGLFAIFSIIRYRTIVVSIKDMGYFFVCV
                     ALGMLNSLASVQDNFKTLIICNVVILLLTFILERLDFIKNENAKEIVYDKITLIKPEF
                     RTELLADLNERTGLKIHRVDVVSIDYLKDVALLKAYFYAKDSENLLPTTVDND"
     gene            7193..7924
                     /locus_tag="P5335_08200"
     CDS             7193..7924
                     /locus_tag="P5335_08200"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRZ74896.1"
                     /translation="MIKTQFKKLLPVFFFVFCYCSIIAQENTDVQYRFYAAADYKITK
                     KWQVAFEYRYAMDHDISEFRNNSFEIGTKYDVAKKMSLETGYRFTTSYEKDSHLLFLS
                     YQYKYKFNKRFTLGSTTRYQFRTLGFDADLMQYYKEPSQFIREKLRLEYNVPKSKASL
                     YFAPEIFLKIDNSLEFNRMRYTLGGDYLLKYGNTLGLSLFYENKYNPQKTDRFVLSTK
                     YNLSIDELIKKINKNKEKKEKKEKI"
     gene            7986..>8872
                     /locus_tag="P5335_08205"
     CDS             7986..>8872
                     /locus_tag="P5335_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRZ74897.1"
                     /translation="MTKNIFPFIVLFSFLSCQNDSDTTIQEVDTYPAVLAVFGNTINL
                     TNLANYANQPVPTYILKDNTNGNTISDKVATLGRVLFYDKNLSSNNTISYSSCHQQIN
                     AFSDTSVSSSGVNGTTGRHSMRLINTRFATETKFFWDERALNLETQTTMPIKDHGEMG
                     FSGTNGDLPFSGLITKLSELDYYKELFQFAYGSEEITESKLQIALAQFIRSIQSFDSK
                     YDAGRALATNDVQPFTNFSAQENQGKNLFLSPPIFDATGSRTGGGLGCAGCHRAPEFD
                     IDPNSGNNGIIGTINATGI"
BASE COUNT         3033 a         1373 c         1349 g         3017 t
ORIGIN      
        1 aaaaaaacat aatacaaatt ttcaacaaat atccaacgta ctgttttata cttgcttaag
       61 caacgcttag tctttactta aaatttgctt aattatagca gcccattata ctttcgaata
      121 aaccattccg aaactaaagc agcaacaaca ataattaata accaaatcca atcaatgata
      181 ggtgtttttt tgacaatttc tttttgtata ggtttataat tatcgttgct aatgagcgat
      241 ttaaccaact cctctacttg atttgcaagg aacgttttac cattggtttg tgtcgctaat
      301 tgatttaatt ttacataatc aggattgaca aattgttttt cgatatcaaa atccaacact
      361 tcaaaaaaac cggcatggga agttttagaa ttattttctg ttacggtaaa tgaataattt
      421 ccggcgggca aaccatctaa atttactttg aatgaattgg ttgatttcag taaatcatag
      481 gatttcgttt gattggttgt tttattttta accgatattg acagccgtgc tttatcatca
      541 aactcataat ttttattaaa atattgtgta tttatttcaa tagcatctcc tgaattataa
      601 aaattttcat gtgtaacaac taatgatttt cgtgcattat tagtggctaa aaactgaatg
      661 gttttatcta taaaaacatc aaatttttca aaactttgtt gatcgatgtg gctttgcaaa
      721 cgccatttcc aactattttc accaaaaagg aaacaagttc ttttcccttg aatatctaca
      781 taacttaata atggtgtatt aagttctaca tttaaaattc gagccgttaa taatgtttcc
      841 atttttattt ttggcgtaat ggtaccaaaa gcattttgca agggtggaaa attttcaaaa
      901 cctatgttat ccgatgcaaa gagattaaat tgtgaggaaa aagtagccaa ataatcctct
      961 tgttgagaac tcattttaaa agaaaaagcg tcttgctgtt gatttaaaaa agtaaaatcg
     1021 gttttagttc ctgtaataat ccatgtattc aagttagcta ctacgttttt ttcgaaaaca
     1081 gttttaaact cagaagtagg ctggtataaa atcaacacat taaaatcttg taatgaattg
     1141 atttgatttg gcttaacaat agttacttta cgttgttgat tagtttcaat agctcttttt
     1201 aaagctccaa tatcaggatg attgattgtg gaaataatag caatttccga tttttgatcg
     1261 atgacttcta ctgcaaaatt cttggaattg ttgtatttat ttttttcaat ttcaggagat
     1321 gaaatggctg ctttaaaaat ctgaacaccc actttgtctg ccggcaataa taccgtaatc
     1381 aagtgtgatt tttggttttg agtaaagttt atattttgtt tatttaaaat agtatttccc
     1441 tgagaaatgg aaaaagtagc attcacgttt ttctttccgg aataatttac aaaaacttca
     1501 acggggaatt tatttttaag aaaagcatat ttattgacat ttaactgact gatttttaaa
     1561 tcaaaaacag ccgttgtatc acccaaaaca atggggtaaa ccgatttgtt tttgtcaaaa
     1621 gaataaatat agtcattccc atacgtttga tttccgtcag tgataatgac tgttggatga
     1681 ttgatgcttt tatgaatact cttcaatcct ttacctactc catctattct ggtctgagtc
     1741 cctttaaaat caaatgtttc agaagcttcc aattcagcag aaaattgata agactgaatg
     1801 ttaaattttt cttttaactc agcgtttgaa actatatttt gataaacttc tagggcaatt
     1861 tcgtttgctt taaactcagc tacagaagat gaattatcaa ctacaaccgg taaaggaatt
     1921 ttgatggttt cgaatgattt tcgagttatg ataggattga ttagtaataa taataatccg
     1981 aaaatggaac aaaaacgcaa aaaagccaag atccaattga tttttgaatt ggatttggct
     2041 ttatagaaat actgaaaaaa tgatactcct ccggccagta atactgaaag taaaagtaaa
     2101 agcacggtgt ttgtggtcat tttttgctaa agtttaagag gttaaaggtt taaaaggtta
     2161 aaagggattg aggttaaatt agtgctccaa atttttaatt tgtgatatgt ataaaccatc
     2221 aattaacata ttaacttcag ctattttttc tcttaaactt aaataattaa tttcatcaat
     2281 gtattcaaaa tcatttgaca aaatcacttg attcaatagc tctaatgatg aactgtaggc
     2341 tatttcagaa aatctagctt tatctttaaa tgatttcctt cgagatcctt ccgctaaatt
     2401 tgaagtgatg gaaatggaac aacgtctcat ttgacttgat aaaccaaatg tctcttcttt
     2461 tggaaacttt tttgttgtct tataaattaa aagagctaat tctcttcctt tttgccaagc
     2521 tattaacttt tcaaaactga atatcctcat atagaatacc ttttaaacct ataaacaata
     2581 aacttataaa cttcctaagt taacattccg ccatccacgt tcatcacttg tccggttaca
     2641 taagcactca aatcggaggc taagaaaaga caagcattgg caacatcttc aggtgttcca
     2701 cctcgtttca aaggaatcga atccctccaa ccttgcacca cttcttcgtt taattttcct
     2761 gtcatttctg tttcgataaa accaggtgca atggcgttgc aacggatgtt tcgtgagcct
     2821 aattccaatg caattgattt agtaaaacca atcataccgg cttttgaagc agcataattc
     2881 gcttgtccgg catttccttt tactccaaca actgaactca tgtttattat agagcctttt
     2941 ctgtttttca acatgatttt ctgaacagct ttggtcatat tgaaaaccga tttaaggttt
     3001 atttcaatga ctttatcaaa atcttcttca gacatacgca ttaaaagatt gtcttttgta
     3061 attccggcat tattaattaa aatatcgata ttcccaaaat cagccatgac ctcgtcggct
     3121 aatttttgag cttcattaaa atcggctgcg tttgatttat atcctttcgc tttgatacct
     3181 atagcattta attcgttttc caaaaccaaa gctgattcgg cagacgaact atacgtaaac
     3241 gccacatttg caccttgttg tgcgaaaact ttagcgattc cgcttccaat tccgcggctt
     3301 gctccagtga taatcgctac ttttccttct aataatttca tagttgaatt tgtttatttt
     3361 gttcaattat ataaaagcca aagataacaa tattttaatc tttgaaaaaa attagannnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnattt taggtcaata catgcatgct
     3541 cctttagcga attatgctaa tgtttacatc aatggaaatt taataggatt atatacaaat
     3601 gtagaatcta tttctaaaaa attcgtgaat actcattttg gttcaaataa taatgccttc
     3661 tttagttgta gtccacctgc aggtgctggt cccggtacaa caaaccttcc taaccttgcc
     3721 tatttaggca caaacagtgc aagttatgaa tcagcttacg aaatcaaatc aacaaccggt
     3781 tgggacgatt taataaattt aacgaataca ttgtcaaaca acacttcaaa tattgaaagt
     3841 gttttagatg tagacagagc tttgtggatg cttgcctttg ataatttatt tgtaaatatt
     3901 gatagttata tcggacaatt taaacaaaac tattacttgt acaaagatga taatggccgt
     3961 tttaatccaa ttttatggga tttaaatatg agtttcggaa cttttggaca aaccggaact
     4021 acaaatttaa atacaaccct tgccaaaaga caattaaacc atttgttaca ttccggagat
     4081 gctgcttggc cattagttca aaaactacta gcagttccaa gatacaaaaa gatgtacttg
     4141 gcacattatg ccactatgtt atcagaaaac atttccaata atagttattt tactaaagca
     4201 caaacctttc aaactttaat caattcatca gttcaagctg atgtaaataa attttatacc
     4261 tatgctcaat tcagcagtaa tatgacaact gatattgcct tagggatgaa taccgcccct
     4321 ggattaagta atttaatgag cggcagaaac acttatttaa cagcattatc agattttacg
     4381 gctacaaaac cctcaatttc tgcaattaca ccatcagtag ccaatcctgc attaggcggt
     4441 tccgttacga ttcgtgcaaa tgtaaccaat acaaatacag atgctgtttt tttaggttat
     4501 cgtgctgctt ctaatttacc atttaccaaa gtattgatgt ttgatgatgg tgcccataat
     4561 gatggtgctt caggtgataa tatttatggt ggattgatgc cgattagttc tatcacctct
     4621 caatattaca tttatgcaga gaacaatact attggggcat tttctccggc aagagcagag
     4681 catgaatttt atactataac ggcttcctat ccaacaatca ctcccggttc tttggtcgtt
     4741 aacgaaataa tggctcaaaa tacaagtacc gtaacagatc caagtggtga ttatagtgat
     4801 tggtttgagt tatataataa cagcagtgtt acaattagtt tagataattt atttgcctct
     4861 gacacttcaa ccaatttatt aaaatggcag tttccatcag gaattacaat ggctccaaac
     4921 agttatttaa ttgtttgggc tgatcaggat ttaacacaag aaggcattca cgctgatttt
     4981 aaattttctg ccagcggaga aagttgtatt ctatcttatg ccaacggaat aatcattgag
     5041 aatgtaactt ttggtgttca ggttgccaac atgggttatg ctcgtgtacc aaatggaaca
     5101 ggtaattttg taatccaggc tcctactttt aatgccaata atcaaggttt gtctatttct
     5161 gattttaatc aacaaaataa tcaattactt atttatccta atcctacttc taattatgta
     5221 aacattagaa tttcagaatt aatgggttct gaaaaaatta aaatttttaa tttattaggt
     5281 caaatagtaa ttgaaaaaga agcacataat gataataccc ttgacttaac aatattaagt
     5341 gccggaacat atgttgtgaa ttatggcaaa atcaataaaa aaatagttgt atttaaataa
     5401 atgtaaaaaa atgaaaaagt tattattact cgtattaata gttacatcat tcactttttc
     5461 ttcttgttcg aaagaagaag gatatggtgg attagcaacc attaaaggaa aagtgtttgg
     5521 aaaagacgtt aattccaatg ggaatttagt gggagaaggt tatgtgggcg gattcaaagt
     5581 atatataagt aaacatggga attcagcatc gtttgatgat gtagattctt cttacgatgg
     5641 aacattccaa tttgagtttt tatacgaagg aacctatgac atttgggtat ttggtgattg
     5701 tgactcttgt gcgtgggatc aagtttatgt attgagaaca gttgaaatta catcaaaaaa
     5761 acaaaccgtt gaagtggaag atttagtcat tacattttaa taaattatgt ttaacaaatt
     5821 tctatccaat ttacagcaat taaatccaac cacattaagt gaattggatt ctgtttcttt
     5881 acaaaacaga atagacagta agtatattct taatgaaaaa gaaatggtca acctcctgcc
     5941 gtgtctaata gagaattata aaattctgac aataaaagat gaaagagttt tctcttatga
     6001 aaacaattat tttgatacat ccgattactt attctttaaa gatcatcaca atggttatgc
     6061 taatagaatt aaagtaagaa gcagaagata tgtagaatct gatttatgtt tttttgaaat
     6121 taaaaaaaag gaaaaagtaa atcggaccaa caaacatcgt gaatcattag ttagtttagt
     6181 ttcagatatc aatgaagaga aaagagaatt aattcaatca tttaccagaa aagaattaat
     6241 tgatttacaa ttaattctga aaaataattt taaaagaata acacttgtta ataatgaatt
     6301 tacagaacga gtaaccattg attttaaaat tcattttgct gatgaaacaa atgaagtcaa
     6361 ttttgaagga attaccgttg ttgaaattaa acaatcaaaa tcttctcaag cctctccttt
     6421 agtatccttt ttaaaagaga gtaatacaag agaacaaagt tttagtaaat acatttttgg
     6481 tgttatttcg ttaaaaccgg aaattaaaaa gaataatttt ttacctatcc ttaaaaatat
     6541 aaataaaata aagaactaat ggaagaagcc gtaaacaccg ttaagaatgc atttgacgca
     6601 accgattttg gaataagagc tgcaattgat tttattgccg ttgttgtttt agtaagttta
     6661 atttattatc caaaacataa aaacaaagac tttattttca cttttgtatt gttcaatgtc
     6721 atcaatttta tgatatgtta tcttttaggt tcagccaaaa taaaaattgg ttttgctttt
     6781 ggattatttg ctattttctc cattattcga tatagaacaa tagttgtttc cattaaagac
     6841 atgggttatt tcttcgtctg tgttgcgttg ggaatgctta attctttggc ttcagttcaa
     6901 gataatttca aaacactgat tatttgcaac gttgtaattt tgttattaac ttttatttta
     6961 gaaagattag attttatcaa aaatgaaaat gcaaaagaaa ttgtctatga taaaataacc
     7021 ttaataaaac ctgaatttcg aactgaactt ttagctgact taaacgaaag aaccggtttg
     7081 aaaattcacc gtgtagacgt cgtttcaatt gactatttaa aagatgttgc cttattaaaa
     7141 gcctattttt atgcaaaaga cagcgaaaac ttgttaccaa caactgtaga caatgattaa
     7201 aactcaattc aaaaaattac ttccggtatt tttctttgtt ttttgttatt gcagtattat
     7261 tgcacaagaa aatacagatg ttcagtatcg tttttatgca gctgctgatt ataagataac
     7321 taaaaaatgg caagtagctt ttgaatatcg ctatgcaatg gatcatgata ttagtgaatt
     7381 cagaaataat tcatttgaaa ttggaactaa atatgatgta gctaaaaaaa tgagtttaga
     7441 aacggggtat agatttacaa cttcctatga aaaagattct catttgctat ttttaagtta
     7501 ccaatataaa tataaattta ataaacgatt tacattaggc tcaaccactc gttatcaatt
     7561 cagaacatta ggttttgatg ctgatttaat gcaatattat aaagaaccat cacaatttat
     7621 aagagaaaaa cttagactag aatataatgt tcctaaatca aaagcgtcac tttattttgc
     7681 tccggaaatt ttcttaaaaa tagataattc acttgaattt aacagaatga gatacactct
     7741 tggaggtgat tatctattaa aatatggaaa tactttaggt ttgtccttat tttatgaaaa
     7801 caaatataat cctcaaaaaa cggatcgttt tgtattaagc actaaatata atttatctat
     7861 tgatgaattg attaaaaaaa tcaataaaaa caaggagaaa aaggaaaaaa aggaaaaaat
     7921 ataattctaa attaaacctt tattaaattc tatagtcata gtaagaccaa ataatactaa
     7981 agactatgac taaaaacatc ttccccttta tcgtattatt ttcctttctt tcttgccaaa
     8041 atgattcaga tacaacaatt caggaagtcg atacttatcc tgcagtttta gcagttttcg
     8101 ggaatacaat taatttaact aatttagcta attatgcaaa tcaacctgtt cccacctata
     8161 tcctaaaaga taatactaac ggaaacacta tctctgataa agttgcaaca ttaggacggg
     8221 ttttatttta tgataaaaat ctatcttcta acaataccat ttcttattca agttgccatc
     8281 agcagataaa tgcctttagc gacacttctg tttccagttc gggtgtaaac ggaactaccg
     8341 gtcgacattc catgcgatta ataaacactc gatttgccac agaaaccaaa tttttctggg
     8401 acgaaagggc gttgaactta gaaacgcaaa cgacaatgcc cataaaagat catggagaga
     8461 tgggtttcag tggtactaat ggtgatttac ccttttcagg attaattacg aaactcagtg
     8521 aacttgacta ttataaagaa ttatttcaat ttgcttatgg ttctgaagaa atcactgaaa
     8581 gtaaattgca aatagcacta gctcaattca ttcgaagtat tcaatccttt gattcaaaat
     8641 atgacgccgg aagagcttta gctacaaatg atgttcaacc ttttaccaat ttttcagcac
     8701 aagaaaatca aggaaaaaat ctatttcttt ctcctccaat ttttgatgca acaggcagtc
     8761 gaaccggagg tggattaggt tgtgccggtt gtcatcgagc tccggaattt gacatagacc
     8821 ccaattccgg aaataatgga attattggaa caattaatgc aacaggtatt ga
//
LOCUS       DAOTCW010000093         8850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_5440_length_8850_cov_43.464810, whole genome shotgun sequence.
ACCESSION   DAOTCW010000093 DAOTCW010000000
VERSION     DAOTCW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5440_length_8850_cov_43.464810"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="P5335_08210"
     CDS             <1..332
                     /locus_tag="P5335_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRZ74898.1"
                     /translation="QWNKFALKLEEKGQMIMHSLMLINDPKLEGTKIIHELPNEGTKL
                     EFERTKNEVLGYLRGMLHNHDIDIELIVNEKMENRRAFTPQDKFNRLNEINPNLELLR
                     KTFDLDI"
     gene            complement(329..1219)
                     /locus_tag="P5335_08215"
     CDS             complement(329..1219)
                     /locus_tag="P5335_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRZ74899.1"
                     /translation="MITIKRYAAIDIGSNAMRLLISNIVEQDGKEPQFNKSSLVRVPI
                     RLGQDVFTIGEISDENINRMIDAMKAFKLLMNIHKVERYKACATSAMREADNNTEIVE
                     IIQQQADVKIDIIDGKKEAAIIASSDLKEYISTDKTYLYVDVGGGSTEFSIFDNGKII
                     ASKSFKNGTVRMLHNMVNEVVWTEIEKWIKIQVEPFDNITMIGSGGNINKLFKMTGKQ
                     QDKPLSYLNLNALYTMLNNMTYEQRISELALNPDRADVIIPATKIYLNAMKWSGARHL
                     YVPKIGLSDGIVKAMYYGKI"
     gene            complement(1240..3366)
                     /gene="ppk1"
                     /locus_tag="P5335_08220"
     CDS             complement(1240..3366)
                     /gene="ppk1"
                     /locus_tag="P5335_08220"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRZ74900.1"
                     /translation="MIKAIEYRYIDREKSWLCFNARVLQEAADPTVPLLDRLRFLGIF
                     SNNLDEFFRVRFAAIRRLSLAGETGEKILGGITAQQLLKDITGIVIKQQSESLRILNI
                     IEKELEKEGVFIINEKEITKDQENYIHDFFIQKVSPALVTIILNDLAEFPLLKDTSGY
                     LAVKLVMKPDSETETSSFLRFKKTKKDIRYAVIEIPKTVNRFVVLPSNGEKQYIILLD
                     DVIRYNLSSIFNIFDYESISAHMIKITRDAQLEIDSDISKSFAEKISESVRDRRIGEP
                     VRFIYDQSIDKDTLKFFLEKMNIDATDSIIPGGRYHNRRDYMDFPNLGRYDLLYQPKT
                     PLPVDGLGLEGSILQKINEKDYLVNAPYQSFAYLIKFLREAALDPKVVSIKITLYRLA
                     KNSQIISSLINAAKNGKKVTVQIELQARFDEASNISYAEQMQTEGINLIFGVKGLKVH
                     SKICVIERVEDKKVKRYGFISTGNFNESTAKVYTDVTLFTSHQQILKDADKVFDFFEV
                     NYRVHRYKHLIISPHYTRNKFYKLIDREIMYALSGKETYIKLKMNSLSDSRMIDKLYD
                     ASRAGVKIQLIIRGICCLIPGIKGMSENIEAISIVDNFLEHSRVFIFGNHGKPEVYIS
                     SADFMTRNIDARVEVTCPIYDENIKLELIESFDVGWKGNVKARFHSEKLDNKYRVRNE
                     NERVFRAQIETYNYYRNQLEVTAEHL"
     gene            complement(3359..3850)
                     /locus_tag="P5335_08225"
     CDS             complement(3359..3850)
                     /locus_tag="P5335_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRZ74901.1"
                     /translation="MKNLILIRHAKSSWEAPVHDKERFLAPRGIQDAHLVSQNIGAFL
                     PKANIIWSSTAKRAAETAVIFAQNLQWPLESIVFKDELYTFDGSKLEKTIKSCQDSYE
                     NVILFGHNEAITEFVNKFGDIFIDNVPTAGFVSISFPQESWSQIKAGKTTKILFPRDL
                     KYD"
     gene            4404..4979
                     /locus_tag="P5335_08230"
     CDS             4404..4979
                     /locus_tag="P5335_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74902.1"
                     /translation="MKRNLIVSVAQQETKSKMRENLSQFFLSFRNSMNVKVLILLVLP
                     LISFAQNVKIENADVFFKSAQLSNVSRSNEINQIKSLIKDLHPSVYLEKGRVNTYGES
                     PKCLFTDVESISLLKDLRTDLSSIEIVTINIKSQKDLTAIIDLDTFLSYPSIKVIHLN
                     VHFDCTADMLYPMIKYVKSNYTVLYSVEKPS"
     gene            5019..>8850
                     /locus_tag="P5335_08235"
     CDS             5019..>8850
                     /locus_tag="P5335_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74903.1"
                     /translation="MKNIYSKLNLNSNYLLLILLVFIGNLYVVNAQIKVPFTQRTSIY
                     TPGKTIYNVKGDFTMIGNTNLTLVNYGDETSNNNNMQYVDIDGDSNTWNSSSSNLTFS
                     TENGANPACSNIVYAGLYWTGRASDGSSSPNIFTVEKQIPGGTQTINQNYTVGHNQAI
                     ANTSYTMTVTRDNPTNTNRSPIYTFTSGSTTYVFRFSNNTGANRVTLSVNGGAAVNVP
                     VSYTTSGSTGTATLTTPYVIANGTVNLRINSLVRDTATNLTTTNTETTSFANVNVAGT
                     IIGTTTVTKTFDKRKVSIKGPNSASYTLLTADDDDIYYPVNGDGFMYSGYVEVTDYVR
                     TNGIGAYTVADVALIEGNGGGTGYYGGWGLVVVYENDKMKWRDVTVFDGHSYVAGSVT
                     ADFEIPVSGFNTAQSGAINMKLGMMAGEGDRGISGDYFQIRNYLDTSWVTLNHGGNST
                     NNFFNSSIFTGGNTRNPNILNNTGLDISMFNIPNTGNSVITNNQTSTRFRYGTTQDTY
                     VIFNLTMSVDAYVPETEGMLSIISIGGVPFNPGDLSSAQPGQEVEFGIDIKNLGTEAV
                     NDYRLVIPIPFTSTYVPGSLNTSIFFSPDPTPNNLYYDANLGATGSLVWDFGTLPLPA
                     DPQTLLASLRFKLRATDNCAILSNPDCVNSIIVLGYTSGTGNITGTPFRNRGLILGYD
                     NSNGSCQGQPIPQPLGVGINAGAFVAQNCQDVPLIRNFSYCNVTEPIPASQLAAQFPP
                     GTKFYATYPVTTPEITSFPVTPGISTTYYAVIPNSNGCHFEFTMSFCGEIIAINDIFN
                     SVECITNGLIGNALENDSVNGVQASSSNVTFTIDSGSNPNITFDAQGNISVTTGIAAG
                     QYVFGYTICEIMNPTNCISATITINIVDTTEPTIAALPAESTINCPATPDFAQAIAND
                     SCGTATLTFVDVRTDGDCAGSYSITRTWTATDASGNISTASQTINVQDVSAPVIADLP
                     IESTINCPAVPQFAQATATDECGYEFTLSFTDVTTNGACAGSYSITRTWTATDTCGNS
                     STATQTINVQDITAPVIADLPSETTISCLPSIVTRFSLNFVEATATDECNSEFTLTFN
                     DVRTDGDCAGSYSVTRTWTATDTCGNSSTASQTINIEDVVAPIINALPAESTIECPAV
                     PEFAEAIASDECGSEFSLSFNDITTSGSCAGSYSITRTWTATDTCGNSSTATQTINVE
                     DNTIPVLVNQGADTTISCPSTPTFSAPTVSDTCDSAPVISFTDTTIAGPCLGTSIVTR
                     TWIATDACGNVSNPV"
BASE COUNT         2906 a         1550 c         1377 g         3017 t
ORIGIN      
        1 tgcaatggaa taaatttgcc ttaaaattag aggaaaaagg gcaaatgatt atgcattcgt
       61 taatgttgat aaatgatccg aaattagaag gaactaaaat cattcacgaa cttcctaatg
      121 aaggcacaaa acttgaattt gagcgaacca aaaatgaagt gctgggttac ttacgtggca
      181 tgttacacaa tcacgatatt gacattgaat taattgtaaa cgaaaaaatg gaaaatcgca
      241 gggcttttac accacaagac aaattcaatc gattgaacga aattaatcct aatttagagt
      301 tgttaagaaa gacgtttgat ttggatattt aaatctttcc ataatacatc gccttcacaa
      361 ttccatccga aagtccaatt tttggaacat ataaatgacg ggctccactc cacttcatgg
      421 catttaaata aatttttgtg gccggtataa ttacgtcggc tcggtcagga tttaaggcca
      481 gttctgaaat acgttgttca taggtcatat tattcaacat cgtataaagt gcattcaaat
      541 tcaaataaga caatggttta tcctgctgtt tacctgtcat tttaaacaat ttattaatgt
      601 tcccaccgga acctatcata gttatattat caaaaggttc tacttgaatt ttaatccatt
      661 tttcaatttc tgtccaaacc acttcgttca ccatattgtg caacatccga accgttccgt
      721 ttttaaatga ttttgaagca attatttttc cattgtcaaa tattgaaaac tctgtgctgc
      781 caccaccaac atcgacatat aaatacgttt tgtcggttga aatatattcc ttcaaatcag
      841 aagaagcaat aatggcagct tccttttttc cgtcaattat atcaattttc acatcagctt
      901 gttgttgaat aatttcaaca atttcagtat tattgtcagc ttcacgcatg gcagaagtgg
      961 cacacgcttt atacctttct actttatgaa tattcatcaa taatttaaag gctttcatag
     1021 catcaatcat tcggtttata ttttcatctg aaatttcacc aattgtaaac acatcctgcc
     1081 ctaaacgaat tggcacacga acaagtgagc ttttgttaaa ttggggttct tttccatctt
     1141 gttctactat attagaaatc aacaaacgca tggcatttga tccaatatcg attgctgcat
     1201 atcttttaat tgtaatcatg aaacgtactt aatttggggt tataaatgtt ctgctgttac
     1261 ttctaattga tttctataat aattataggt ttcaatttga gctctgaaaa cccgttcatt
     1321 ttcatttcga actcggtatt tattgtctaa tttttcggaa tgaaatcggg ctttcacatt
     1381 acctttccaa cccacgtcaa aactttcaat cagttcaagt ttaatatttt catcatatat
     1441 aggacaggtt acttctacac gagcgtcaat attacgtgtc ataaaatcgg ctgaggaaat
     1501 ataaacttcc ggttttccat gattaccaaa aataaatacg cgggaatgtt ctaaaaaatt
     1561 atcaacaata ctaatagctt caatgttttc gctcattcct tttatacctg gaattaaaca
     1621 acaaattcct ctgatgatga gttgaatttt cacacctgct ctactagcat catataattt
     1681 atcaatcatt cttgaatcag ataaactatt catttttagt ttaatgtatg tttcttttcc
     1741 ggaaagagca tacattattt cacgatcaat taatttataa aacttatttc tggtataatg
     1801 cggagaaata atcaaatgtt tatagcgatg tactcgatag ttaacttcaa aaaaatcaaa
     1861 tactttatca gcatctttta aaatttgttg gtgactggta aataaagtta catctgtgta
     1921 tactttggct gttgattcat tgaaatttcc ggtagaaata aaaccataac gtttcacttt
     1981 tttatcttcc actcgttcga tgacacatat cttactgtgc acttttaacc cttttacacc
     2041 aaaaattaaa ttaatgcctt ccgtttgcat ttgttctgca taggaaatat tactagcttc
     2101 gtcaaaacga gcttgtaatt caatttgaac agtcactttt tttccgtttt ttgcggcatt
     2161 aataagcgag cttatgattt gagaattttt tgccaatcga taaagtgtga tttttatact
     2221 aacaaccttt gggtcaagag cggcttcccg caaaaattta attaaatagg caaacgattg
     2281 ataaggtgca tttactaaat aatccttttc atttattttt tgcaaaatgc tgccttccag
     2341 cccaagtcca tctaccggta atggtgtttt tggttgatat aataaatcgt aacgtcctaa
     2401 attaggaaaa tccatataat cgcgacgatt gtggtatctt cccccaggaa taatactatc
     2461 cgtagcatca atattcattt tttctaagaa aaacttcagc gtgtctttgt caattgattg
     2521 gtcataaata aacctaaccg gctctcctat tcttcgatct cgtacacttt cagatatttt
     2581 ttcggcaaaa gatttactaa tatcgctatc tatttccaac tgagcatcac gtgtaatttt
     2641 tatcatatga gccgaaatac tttcataatc aaaaatatta aaaatactac ttaggttata
     2701 ccgaatcaca tcatctaata aaatgatata ttgtttttcg ccattagaag gtaaaaccac
     2761 gaatcgattc actgtttttg gaatttctat aacagcataa cgaatatctt ttttcgtttt
     2821 tttaaacctt aaaaaagatg atgtttccgt ttcagaatca ggcttcatca ctaatttgac
     2881 tgccaaataa cccgatgtat ccttcaataa tgggaattcc gctaaatcat ttaatatgat
     2941 agttactaat gccggactaa ctttttgaat aaaaaaatcg tgaatataat tctcttggtc
     3001 ttttgtaatt tctttttcat taataataaa aacaccctct ttttcaagct ctttttctat
     3061 tatattcaaa atacgcaaac tttcagattg ttgtttgata acaattccgg taatatcctt
     3121 caaaagttgc tgagcagtaa ttccacctaa aattttttct ccggtttcac cggctagcga
     3181 taaacgtcgg attgcagcaa aacgaaccct gaaaaattca tctaaattat tggaaaaaat
     3241 tcctaaaaat cttagtcggt ctaataaagg aactgtcgga tctgcagctt cctgaagaac
     3301 tctagcgtta aaacataacc aacttttttc tctatcaata taacggtatt caattgcttt
     3361 aatcatattt taaatctctt ggaaaaagta tttttgttgt ttttcctgct ttgatttggg
     3421 accaactttc ttgtgggaag gaaattgaca caaatccggc agtaggaaca ttgtctataa
     3481 aaatatctcc aaatttatta acaaattctg tgattgcctc attatgtccg aaaagaataa
     3541 cattttcata actatcctga caagatttta ttgttttttc aagttttgaa ccatcaaagg
     3601 tgtaaagctc atctttaaaa acaatacttt ctaaaggcca ttgcaaattt tgagcaaaaa
     3661 taacagctgt ttcagctgca cgttttgctg tactactcca gataatattt gccttaggca
     3721 aaaatgcacc tatattttgc gataccaaat gagcatcctg aatacctctt ggagctaaaa
     3781 atctttcttt atcgtgaacc ggtgcttccc aactggattt tgcatgtcga attaaaatta
     3841 agtttttcat cccgaaaaaa tttaaattta aacataaaaa tattttacaa tttaagcatt
     3901 tatttcaata attgaaacaa ttttcaaatt gaaattaaat attgtctatt aaaagttgaa
     3961 ttttaacaat attcctattt ttttaatcta gttttctttc atagataagt tataaacata
     4021 ttatcaacaa ccgaaaaaat atatttaaaa accgtttttt cttacagaat atttatttta
     4081 actcacttta tcgtcaaaaa aatgcatttt atcgataaaa tatttttttt attcaaactt
     4141 tcattttaca ttcgtctaga atttaagaat taattaactc taaaattcgt caaaaatcaa
     4201 tttaagaatt aaaaaaacag tagttattta atgccaaatt aatctatgaa atgcatctgt
     4261 tgtgtaattt atcgagtatt ttggttagtc acgggaaaaa tgagcaataa cgtaaattag
     4321 tatatagctt agctcccgtt aattaaatag cctgtaaaag gtgaaaaaaa ttaacaaacc
     4381 caattcaacc taattattta attatgaaaa gaaacctaat tgtttctgtt gcccaacaag
     4441 aaactaaaag caagatgagg gaaaatctgt cccaattctt tttgagtttt cgtaactcaa
     4501 tgaatgtcaa agtacttata ttattagtac tacctcttat tagctttgca caaaatgtta
     4561 aaattgaaaa tgctgatgtt ttttttaaat cagcacaatt atcaaatgtg tcgagaagta
     4621 acgagataaa tcaaatcaaa tctttgatta aagatttaca cccatcggtt tatttggaaa
     4681 aaggcagggt gaatacttat ggcgaatctc caaaatgttt gtttacagat gtagaatcta
     4741 tttcacttct aaaagactta agaacagact tgagttcaat agaaattgta acaatcaata
     4801 ttaaaagtca aaaagactta actgctataa ttgatttaga tacattttta agttatcctt
     4861 caatcaaagt gattcatttg aatgtacatt ttgattgtac tgcggatatg ttgtatccaa
     4921 tgataaaata tgtaaaatca aactacactg ttttatatag tgttgaaaaa ccttcctaac
     4981 attcatttaa aggaaattaa tccgataaag attatatcat gaagaatatt tactcaaaac
     5041 taaacctaaa ctctaattac ctactactaa ttttattagt atttattggt aatttgtatg
     5101 tggttaatgc tcagataaag gttcccttta cgcagcggac gtcaatttat acccctggca
     5161 aaaccattta caatgtaaaa ggtgatttta ccatgattgg taataccaac cttacactag
     5221 tcaattatgg agacgaaacc tctaacaata acaacatgca atatgttgat attgatggtg
     5281 attccaatac ttggaattct tcgtcctcta atctaacttt ttcaactgaa aatggtgcga
     5341 atcctgcctg ttcgaacatt gtttatgcag gtttgtattg gaccggtaga gcatctgatg
     5401 gctcaagcag tcctaacatt tttactgtcg aaaaacaaat accgggtggt actcaaacca
     5461 tcaatcaaaa ttatacggtt ggacataatc aggctatagc aaacacaagc tatacaatga
     5521 cggtaaccag agataatcca acaaatacaa atcgtagtcc tatttatact tttacaagtg
     5581 gatcaactac ctatgtattt agattctcga ataatacggg tgcaaaccgt gttactctat
     5641 ctgtaaatgg cggagctgct gtaaatgttc ctgtaagtta tacaacttca ggttcaacag
     5701 gaacggcgac tttaactact ccttatgtta ttgcaaatgg taccgttaat cttagaatca
     5761 atagtttggt aagagatact gctaccaatt taacaacaac aaatacagaa acaacatcat
     5821 ttgcaaatgt aaatgttgcc ggaactatca ttggtaccac aacagttaca aaaacatttg
     5881 ataagagaaa agtatcaatc aaaggtccta actcagcaag ttacactctt ttaacagctg
     5941 acgatgacga tatatattat cctgtcaatg gtgacggatt tatgtattcc ggttatgttg
     6001 aagtaaccga ttatgttaga acaaacggaa ttggtgctta tacagtagct gatgttgctt
     6061 taattgaagg taatggtggc ggtaccggat actacggtgg atggggatta gtagttgttt
     6121 atgaaaatga taaaatgaaa tggagagatg taactgtttt tgacggtcac tcttatgttg
     6181 ccggaagtgt aactgctgat tttgaaattc cggtatctgg ttttaatact gctcaatcgg
     6241 gagctatcaa catgaaatta ggaatgatgg ccggtgaagg tgatcgcgga atttccggtg
     6301 attattttca aattagaaat tatcttgata caagttgggt aacgctaaac catggaggaa
     6361 atagtaccaa caacttcttt aattcatcca tctttacagg aggaaatact agaaatccta
     6421 atattttaaa caatacaggt ttagatatta gtatgttcaa tataccaaat acaggaaact
     6481 ctgtaatcac taacaatcag acttcgactc gatttagata tggtactaca caagatactt
     6541 atgttatttt taacctaacc atgtctgttg atgcttatgt tcctgaaaca gaaggtatgt
     6601 tgtcaatcat aagtattgga ggagttccct ttaatcccgg agacttaagt agtgcacaac
     6661 cgggtcaaga agttgagttt gggatagata ttaaaaacct tggaactgaa gcagtaaatg
     6721 actacagatt ggttattcca attccattta cttcaacata tgttcctgga agtttaaaca
     6781 catctatatt tttctcacca gatccaactc ctaataattt atattatgat gcaaatcttg
     6841 gagctactgg ttcattagta tgggattttg gaacgttacc gttacctgct gatcctcaaa
     6901 cattattagc ctctttacgt tttaaattaa gagcaaccga taattgtgct attctttcta
     6961 atccggactg tgtaaatagt attattgttt taggatatac ttccggtact ggaaacatta
     7021 caggaacacc ttttagaaac agaggtttaa ttttaggata tgataattca aacggaagtt
     7081 gtcaaggtca accaatacca caacctcttg gggttggtat aaatgccggt gcttttgtag
     7141 ctcaaaattg tcaagatgtt ccgttaataa gaaatttttc ttattgtaat gtaacagaac
     7201 ctattcctgc ttcacaatta gccgcacaat tccctccggg aactaaattc tatgctacgt
     7261 atcctgttac aacaccggaa atcacatcat ttcctgtgac tccaggtatt tcaactacct
     7321 attatgctgt aattcctaat agtaacggat gtcattttga atttacgatg tcattttgtg
     7381 gagaaattat tgctattaac gatattttta actccgtaga atgtataaca aacggattga
     7441 taggtaatgc tttagaaaat gattctgtaa acggtgttca agcttcatct tctaatgtta
     7501 catttacaat agattccgga agtaatccaa atatcacatt tgatgcccaa ggaaatattt
     7561 cagttaccac tggaatagca gctggtcaat atgtttttgg atatactatt tgtgaaataa
     7621 tgaatccaac aaattgtatt tcagcaacga taacaataaa tatagttgac accactgaac
     7681 caacaattgc agcgttacct gcagaatcta ccattaattg tcctgccacg cctgattttg
     7741 ctcaagctat agcaaatgat agttgtggaa cggcaacatt aacctttgta gatgtcagaa
     7801 ctgatggtga ttgtgccggt tcttattcta ttactagaac ttggacggcc actgatgctt
     7861 caggaaatat ttcaactgca tctcaaacta taaatgttca agatgtttca gctccagtta
     7921 ttgcagattt accaattgaa tctactataa attgtcctgc ggttcctcaa tttgctcagg
     7981 caactgctac tgacgaatgt ggatatgaat ttaccttaag tttcacagat gttactacta
     8041 atggtgcttg tgccggttca tattctatta caagaacttg gacggctact gatacttgtg
     8101 gaaattcatc tacagcaact caaactataa atgtacaaga tattacggct ccagttattg
     8161 cagatttacc gagtgaaaca acaattagtt gtttaccatc tattgtaaca agatttagtt
     8221 tgaattttgt tgaggcaacg gctacagacg aatgtaattc tgagtttact ttaactttta
     8281 atgatgtaag aactgatggt gattgtgcag gttcatattc tgtaaccaga acatggacag
     8341 ctacggatac ttgtggaaat tcttctactg cttctcaaac aataaatatt gaagacgtag
     8401 ttgctcctat tattaatgct cttccggctg aatcaactat tgaatgtcct gcagtacctg
     8461 aattcgcaga agcaatagct tctgacgaat gtggctcaga attttcgctt agttttaatg
     8521 acattactac ttcaggatca tgtgccggtt catactctat caccagaact tggacggcta
     8581 cagacacttg tggaaattct tctacagcaa ctcaaacaat aaatgttgaa gataatacta
     8641 ttccagtttt agtaaatcag ggagctgata caacaattag ttgtccttca actccaactt
     8701 tttcagctcc tactgtttct gacacttgtg actctgctcc ggtaattagt tttactgata
     8761 caactattgc aggtccttgt ttaggaactt ctattgtaac tagaacttgg attgcaaccg
     8821 atgcttgtgg taatgtttct aatccggtaa
//
LOCUS       DAOTCW010000094         8743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_5535_length_8743_cov_47.056400, whole genome shotgun sequence.
ACCESSION   DAOTCW010000094 DAOTCW010000000
VERSION     DAOTCW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5535_length_8743_cov_47.056400"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="P5335_08240"
     CDS             complement(<1..971)
                     /locus_tag="P5335_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRZ74904.1"
                     /translation="MLSLIKSLRGDKVIWALVALLALFSFMPVFSASSNLAYAGKGTG
                     NTVSYLVKHLVHVALGFYIIYRVHKIPYHYFRSISRIVLPFIWVLLAYTLFKGTVIAG
                     ANASRWIQVPFVGISFQPSAFAFIVLMIFVARYLSKTREEPITFKSSFIDLWLPVGIT
                     LMFILPANFSTAALIFAMILMLVFIGKYPLKYIATVLGIGLVALTLFVLIAKAFPNPL
                     TTRVNTWEARIERFVKDDVTDLDEVYQIERAKTAIASGGVYGLGPGKSVQKNFLPQSS
                     SDFIYAIIVEEFGLFGGLAILLIYLLLFLRFIVAAHKANTLFGKLVVV"
     gene            complement(988..2319)
                     /gene="murD"
                     /locus_tag="P5335_08245"
     CDS             complement(988..2319)
                     /gene="murD"
                     /locus_tag="P5335_08245"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRZ74905.1"
                     /translation="MRLVVLGGGESGVGTAILGKKKGYDVFLSDFGKIKENYKEVLVL
                     NDIAWEDEKHTEELILNADVVMKSPGIADKVAIVKKLHEKQIPVISEIEFASQFTKAI
                     TIGITGSNGKTTTTMLTYHLLKSGGLNVGLAGNIGKSFAWQVADEDFDVYVLELSSFQ
                     LDGIIDYKPHIAIITNISPDHLDRYDYKYENYIASKFRITMNQTEEDYLIYDADDEAI
                     NNWLQNNKTKAKLVPFSLTKKHETGGFINNDTMEMYINEEEFTMETKTMALEGKHNMK
                     NAMAATSVAQLMRIRKQTIRESLSNFQGVEHRLERVLKIQNVQYINDSKATNVNATFF
                     ALDSMTTPTVWIVGGVDKGNDYAELMAMVREKVKAIICLGVDNKKIIDAFGNVVDVMV
                     EVSSMSDAVRTAQHLTEKGDTVLLSPACASFDLFENYEDRGRQFKQAVRNL"
     gene            complement(2321..3550)
                     /gene="mraY"
                     /locus_tag="P5335_08250"
     CDS             complement(2321..3550)
                     /gene="mraY"
                     /locus_tag="P5335_08250"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRZ74906.1"
                     /translation="MLYYLFDYLDKSLDVPGTGVFQYITFRSGLAVLLSLLLSTIFGK
                     RIIEFLRNQQVGETVRELGLEGQTQKAGTPTMGGLIIILATLVPVFLLAKLDNIYIVL
                     LVVTTLWMGTIGFIDDYIKIFKKDKEGLKGRFKVIGQVGLGLIVGSVLYFHPSVTVRK
                     DTQRTNILQQTTETVVSAVPLEEKSTATTIPFFKNNEFDYADMLSFLGDGYETWAWLI
                     FIPIVIFIITAVSNGANLTDGIDGLAAGTSAISVLTLGIFTFVSGNIIFSNYLNIMYI
                     PNSGEMTVFISAFVGSLIGFLWYNSYPAAVFMGDTGSLTIGGIIAVIAIAIRKELLIP
                     VICGIFLAENLSVVLQVSYFKYTKKKFGEGKRIFLMSPLHHHYQKKGYHESKIVTRFW
                     IVGILLAILSIVTLKLR"
     gene            complement(3558..>4133)
                     /locus_tag="P5335_08255"
     CDS             complement(3558..>4133)
                     /locus_tag="P5335_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRZ74907.1"
                     /translation="YNLLAIYGTAVELGLDSLEVLRLLSELESVSGRFQFIVSDSKIT
                     AIVDYAHTPDALENVLGTINDIRTKNEQLITIVGCGGDRDKTKRPIMANIATSLSDKV
                     ILTSDNPRSEDPTVILLEMEKGVEPQNHRKTLSILDRKQAIKTACQLAQPNDIILIAG
                     KGHETYQEIKGVRHHFDDMEIVKELLDEQNK"
     assembly_gap    4134..4233
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4234..4637)
                     /locus_tag="P5335_08260"
     CDS             complement(<4234..4637)
                     /locus_tag="P5335_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRZ74908.1"
                     /translation="MITLKDILYKVNIEAVKGSTEATINKIEFDSRKVELNDVFVAIR
                     GTISNGHDFIEKALNQGASVIVCDTFPELIVNGVTYILVKDTNLALAFLAANFYDNPS
                     QNLKLVGITGTNGKTTIASLLYQLFKKAGFKVG"
     gene            complement(4634..6628)
                     /locus_tag="P5335_08265"
     CDS             complement(4634..6628)
                     /locus_tag="P5335_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HRZ74909.1"
                     /translation="MAVEDKHISYRIYLVAFVILMLAIAITVKLTNIQWVEGEYYRQL
                     AKERSVKNFVIPANKGNIYSSDGSLLATSIPNYKVRFDAIAPNKVDFDKNLQPLCDSL
                     AKLLGKPTQFYKTELLRARADKNRYYLVARNLSYTQYMKIKSFPLFNKGAYKGGIITE
                     QRTIREHPIGLVAERTIGYERIKPDGKPDGKGIEWAFKDYLTGKDGKRLMQKMAKGQW
                     KPIRDENEVEPQDGYDIISTLDVYIQDIAHHALLKQLEYYEADHGCVVVMETKTGEVK
                     AISNLGRNQEGQYTETVNYAVAESHEPGSTFKLVDLLALFEDKLADTNTVYDSKGGEI
                     TYYNRRVRDSKKGGYGKITLARGFEVSSNTIMVQAVYDNYKNNPKKYVDHINRMKLNQ
                     PLGLQIKGEGKPIIPQPGAKNWYGTTLPWMAYGYGVSLTPLQTLTLYNAVANNGEMVK
                     PLFVKEIKEWNKTIKKFDKVVINPKIASQETIDKVKVVLENVVKRGTGSKLYSKDFSM
                     AGKTGTAQVGYADKANLYYASSFVGYFPADNPKYSCIVVVHKPSTSKGYYGADVSGPV
                     FKRIAQKVYTDVPSTNEIKNLEKSNLVQDKNYETYFTNSSKNMQTIPNVHGMPGMDAV
                     ALLENLKVKVKVIGIGKVKRQSIQPGQPLLNNTTIILELS"
     gene            complement(6619..6966)
                     /locus_tag="P5335_08270"
     CDS             complement(6619..6966)
                     /locus_tag="P5335_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HRZ74910.1"
                     /translation="MKAGIYDILKAKFLINEDAAKNWKFIVFLIALAMIMIANIHWYE
                     KKIFQIVEMNKEVKELRSEFVDRRSELMKLKMESTISKKMEEKGIFPSTVPPTKIKVA
                     KPKEKTLTEKIWQ"
     gene            complement(6967..7875)
                     /gene="rsmH"
                     /locus_tag="P5335_08275"
     CDS             complement(6967..7875)
                     /gene="rsmH"
                     /locus_tag="P5335_08275"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRZ74911.1"
                     /translation="MKMTMEYHNPVLLQASVEGLNIKPDGVYVDVTFGGGGHSKEILK
                     HLGSNGKLFAFDQDEDALANALPDERFKLINENFRNIKRFLRFYGVKEVDGILADLGV
                     SSHQFDVAERGFSTRFDAELDMRMNQKDSLNAFTVVNEYEEVRLKKIFTEFGELSNAG
                     AIANTIVNARKDSPIKNTEQLKQVLSRFLPAHKSHKILAQIYQAIRIEVNQEMEVLKE
                     FLVQALDVLKPEGRLSVISYHSLEDRLVKRFMKNGLFDGEPERDFFGNFSVPFKSIGK
                     LIVPDNKEIKINNRARSAKLRIAEKK"
     gene            complement(7850..8323)
                     /gene="mraZ"
                     /locus_tag="P5335_08280"
     CDS             complement(7850..8323)
                     /gene="mraZ"
                     /locus_tag="P5335_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRZ74912.1"
                     /translation="MNTIVGTYECKVDAKGRLLLPAPLKKQLASSLQDGFVLKRSVFQ
                     PCLELYPMAEWNVLMNKVNKLNRFVKKNNDFIRRFTAGVKIVEIDALGRLLVPKDLVV
                     FASVSKDIVLSSAVNIVEIWDKDLYEKSIDDATVDFADLAEDVMGNVNEDDNGIS"
BASE COUNT         2737 a         1710 c         1221 g         2975 t
ORIGIN      
        1 actaccacta attttccaaa taatgtattg gccttatggg ctgctacaat aaaccggaga
       61 aataataaca agtaaatcaa caaaatagct aaacctccga acaaaccaaa ttcttccact
      121 ataattgcat aaataaaatc ggaggaagat tgtggtaaga aatttttctg aacactttta
      181 ccaggcccta atccataaac accaccagat gcgatagctg tttttgcccg ttcaatttga
      241 taaacttcat ctaaatctgt aacatcatct tttacgaaac gttcaattct agcttcccaa
      301 gtatttacac gcgttgttaa tggatttgga aatgcttttg ctattaacac aaacaaggtt
      361 aaggctacaa gaccaattcc taaaacagta gcaatatatt ttaaaggata tttacctatg
      421 aaaaccaaca tcaaaatcat tgcaaatatc aaagctgctg ttgaaaagtt agccggtaaa
      481 ataaacatca atgtaatgcc aaccggtagc cataaatcaa taaaagagga tttaaatgta
      541 atgggctctt ctcttgtttt agacagataa cgtgctacaa aaatcatcaa tacgataaaa
      601 gcaaaagcag aaggctggaa tgaaattcca acaaaaggaa cttgaatcca acgactggca
      661 tttgcaccag ctattaccgt tcccttaaat aatgtgtaag ccaaaagcac ccaaataaag
      721 ggcaaaacta tgcgggatat acttctaaaa taatgatatg gaattttatg aacacgataa
      781 ataatataga aaccgagagc tacatgaacc aaatgtttca ctaaataact aactgtattt
      841 ccggttcctt ttccggcata ggctaaatta ctgcttgcac taaaaaccgg cataaaagaa
      901 aacagagcta atagagcaac cagtgcccaa atgactttat ctcctcttaa acttttgatt
      961 aatgaaagca tacttcttta tctaatttta taaatttcta accgcttgtt taaattgtct
     1021 tcctctgtct tcgtaatttt caaataaatc aaaactggca caagccggtg ataataagac
     1081 ggtatctcct ttttcagtca gatgttgagc tgttcttacg gcatcactca tggaagaaac
     1141 ctctaccatc acatcaacaa cattaccgaa agcgtctata atttttttat tatctacacc
     1201 caaacagata atcgctttca ctttttcacg taccattgcc atcaattcag catagtcatt
     1261 tcctttatca acccccccaa caatccaaac ggttggtgtt gtcatactat ctaaagcaaa
     1321 aaatgtagca ttcacattcg ttgctttact atcgttaata tattgtacat tttgaatttt
     1381 caaaacccgt tctaatcggt gttcaactcc ttgaaaattt gacaagcttt ctctaatggt
     1441 ttgttttcta attctcatca gttgggctac cgaagtggct gccatagcat ttttcatgtt
     1501 atgtttgcct tccaaagcca ttgttttggt ttccattgta aattcttctt cgttgatgta
     1561 catttccata gtgtcgttgt ttataaatcc tcctgtttca tgtttttttg tcaatgaaaa
     1621 aggaactaat tttgcttttg ttttgttgtt ttgtaaccaa ttattaattg cttcatcgtc
     1681 agcatcataa atcaaataat cctcttctgt ttgattcatt gtaattctga atttagaagc
     1741 aatataattt tcatacttat aatcatatcg atctaaatga tctgggctga tgtttgttat
     1801 tattgcaata tgtggcttgt aatcgataat tccatccagt tgaaaactac ttaattccaa
     1861 cacatataca tcaaaatctt catcagctac ttgccatgca aagctttttc caatatttcc
     1921 tgctaatccc acatttaaac ctcctgattt taggagatgg taggtaagca ttgttgttgt
     1981 tgttttacca ttacttcctg taattccaat tgttatagcc ttggtaaatt gagatgcaaa
     2041 ttcaatttca gaaattacag gaatttgttt ttcatgtaat ttttttacta ttgcaacttt
     2101 gtcagcaatc cctggacttt tcataactac atcggcattt aaaatcaact cttccgtatg
     2161 tttttcgtct tcccaagcaa tatcatttaa aaccagaact tctttgtaat tttctttaat
     2221 ctttccaaaa tcagacagaa aaacatcata tccttttttc tttcctaaaa tagcggttcc
     2281 tactccactt tctcctccac ctaaaacaac taatcgcata ctatcttagt ttaagggtta
     2341 caatggatag aatggctaac aatattccca caatccaaaa acgggttaca attttacttt
     2401 catggtatcc ttttttctga taatgatgat gtaaaggtga catcagaaat attctttttc
     2461 cttcaccaaa tttcttcttg gtatacttaa aatagctgac ttgtaaaacc actgataaat
     2521 tttctgctaa gaagattcca cagattactg gaattaaaag ctcttttcga atagcgattg
     2581 caattacagc aataattcca ccgatggtta aacttccggt atcgcccata aatactgctg
     2641 ccggataact gttgtaccac aaaaacccga tgagagaccc cacaaaagca gaaatgaaga
     2701 ctgtcatttc acccgaattt gggatataca taatattcag gtaattagag aaaataatat
     2761 taccggaaac gaatgtaaaa atccctaaag tgagcacaga aatagcagaa gttccggctg
     2821 cgaggccatc aattccatcc gttaaattag ctccgtttga aactgctgtg attataaaaa
     2881 tgacgatagg tataaaaatt aaccatgccc aagtttcata tccatcacct aaaaaagaca
     2941 acatatcagc ataatcaaat tcattatttt taaaaaacgg aatggttgta gccgttgatt
     3001 tttcttctaa agggactgca gacacaactg tttcagtagt ttgctgcaaa atattagttc
     3061 gttgagtgtc ttttcgaacg gtcacactag gatggaaata aagtacagat ccaactatta
     3121 atcctaatcc cacttggcct attactttaa accttccttt taaaccttcc ttgtcttttt
     3181 tgaaaatttt gatataatcg tctataaaac cgattgttcc catccacagc gtggtcacaa
     3241 ccaataaaac aatataaata ttatctaatt tagcaagtaa aaataccgga actaaagtgg
     3301 ccaaaatgat gattaatcca cccatggttg gtgtacctgc tttttgcgtt tgaccttcta
     3361 aaccaagttc acgaactgtt tccccaactt gctggtttct taaaaattca atgattcttt
     3421 taccgaaaat agttgacaat aaaagagaca ataaaaccgc caatccagaa cgaaatgtaa
     3481 tgtattgaaa cactccagtt cccggaacgt cgagtgattt gtctaaataa tcaaataaat
     3541 aatacagcat atcggttcta tttgttttgt tcgtctaaca attctttaac tatttccata
     3601 tcgtcaaaat gatgacgcac tccttttatt tcctgatacg tttcatgtcc ttttccggca
     3661 attaagataa tgtcgttagg ttgagccaat tgacaagcag ttttaatcgc ttgttttcta
     3721 tctaaaattg aaagtgtttt gcggtgattt tgaggctcca cccctttttc catttccaat
     3781 aaaattactg ttggatcttc gcttcttgga ttatcggaag taaggataac tttatcgctc
     3841 aatgaggtgg caatgtttgc cataatcgga cgctttgttt tatctctatc gcctccgcaa
     3901 ccaacaattg tgattaattg ctcatttttg gttcgaatat cgttgatagt tcctaaaaca
     3961 ttttctaaag catcaggtgt gtgggcatag tcaacaatag ctgtaatttt gctgtcagag
     4021 acaataaatt gaaatctacc ggaaacgctt tctaattccg ataacaatcg taaaacctcc
     4081 aagctatcaa gacctaattc aaccgcagtt ccgtagatag ccaataaatt atannnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncctactt taaaaccggc ttttttaaat
     4261 aattgatata ataaagaggc aatagttgtt tttccatttg ttccggtaat accaactaat
     4321 tttaaatttt gtgatggatt atcataaaaa ttagccgcta aaaaagcaag tgctaaattg
     4381 gtgtctttta ccagaatata agttactcca ttaacaataa gttcaggaaa agtatcacac
     4441 acaattacgg aggctccttg attcaatgct ttttcaataa aatcatgtcc gttcgatatt
     4501 gtccctctaa tagccacaaa gacatcattt aattccactt ttctggaatc aaattcgatt
     4561 ttattgatag tagcttccgt tgaacctttc actgcttcaa tgttaacttt atataatatg
     4621 tcttttagcg tgatcacgat aattctaaaa taattgttgt attatttaat aagggttgcc
     4681 cgggttgtat ggattgtctt tttacttttc ctattccgat aacttttact tttactttta
     4741 aattttccaa aagtgcaaca gcatccattc ccggcattcc atgaacatta ggaatagttt
     4801 gcatattttt tgaagaattt gtaaaatacg tttcgtaatt tttatcctga actagattac
     4861 ttttctctaa gtttttgatt tcattggtgg aaggaacatc tgtataaact ttttgagcaa
     4921 ttcttttaaa taccggtcca gaaacatcag ctccataata accttttgat gtacttggtt
     4981 tatgcaccac tacaatgcaa gaatattttg gattatctgc cggaaaataa cctacgaaag
     5041 aagatgcata atataagttt gccttatccg catagcccac ctgagcagtt ccggtttttc
     5101 cggccattga aaaatccttt gaatataatt tagacccggt tcctctttta actacatttt
     5161 ccaaaaccac ttttactttg tcaattgttt cttgtgatgc aatttttgga ttgatgacca
     5221 ctttatcaaa ctttttaatg gttttattcc attctttaat ttctttgaca aaaagaggtt
     5281 tgaccatttc tccattatta gccaccgcat tataaagcgt caacgtttga agcggtgtca
     5341 aggaaactcc gtacccgtat gccatccaag gcaaagttgt accataccag ttttttgctc
     5401 ccggttgagg aattataggc tttccttctc ctttgatttg aagtcccaat ggctgattta
     5461 atttcatccg attgatatgg tctacatatt ttttgggatt atttttataa ttatcataaa
     5521 ccgcttgtac cataatcgtg tttgaggaaa cttcgaaacc gcgagctaat gtaattttac
     5581 cgtatccgcc ttttttagaa tcacgtacac gacgattata ataagtaatt tcaccacctt
     5641 ttgaatcata aaccgtgtta gtatcggcca atttatcctc aaaaagagcc aataaatcca
     5701 ctaatttgaa agttgaaccc ggttcatgag attcggcaac agcataattt acagtttccg
     5761 tgtattgtcc ttcttgattt cttcctaagt tggaaattgc ttttacttca ccggttttgg
     5821 tttccattac aacaacacaa ccatggtcag cttcgtagta ctctaattgt tttaataaag
     5881 catggtgggc aatatcttga atataaacat ccaaagtcga aataatatca tagccatctt
     5941 gtggttctac ttcattttca tcacgaatag gcttccactg acctttggcc attttttgca
     6001 tcaaccgctt accgtctttc ccagtcaaat aatctttaaa agcccattca attccttttc
     6061 catccggttt tccatccggt ttaattcttt cgtaaccaat agttctttcc gctactaacc
     6121 caattggatg ttcacgaatg gttctttgtt cagtaataat tccaccttta taagctcctt
     6181 tgttaaacaa tggaaagctt tttatcttca tgtattgcgt ataacttaaa tttcgggcaa
     6241 ccaaataata cctgttttta tccgctctgg ctctcaataa ttccgtttta taaaattggg
     6301 ttggtttccc taaaagttta gctaaagaat cacaaagcgg ttgcaaattt ttatcaaaat
     6361 ctactttatt tggagcgatt gcatcaaatc gaactttgta atttggaata gaagttgcca
     6421 acaaactacc atctgaagag tatatatttc ctttattagc agggataaca aaatttttaa
     6481 cacttctttc tttcgctaat tgtcgataat attctccttc tacccattgg atattagtca
     6541 atttaacagt aatagcgata gccaacatta gaatgacaaa tgctaccaaa taaattctgt
     6601 aagagatatg tttatcttct actgccatat tttttctgtc aaggttttct cttttggttt
     6661 agctactttt atttttgttg gaggcactgt ggagggaaaa attccttttt cttccatttt
     6721 tttggatatg gtgctttcca tttttaattt cattaattca gatcggcggt caacgaactc
     6781 ggaacgcaat tcctttactt ccttattcat ttcaacaatt tgaaatattt ttttttcata
     6841 ccaatgaatg ttggcaatca taatcatggc taaagcaatt agaaaaacaa tgaatttcca
     6901 gtttttagct gcatcttcat tgattagaaa ttttgctttt agtatgtcat aaattccggc
     6961 tttcatttac tttttttcag ctattcgcaa ctttgcactt cgggctctgt tattgatttt
     7021 tatttctttg ttatcaggca ctattaattt tccgatggac ttaaaaggaa cagaaaaatt
     7081 tccaaaaaaa tcacgttccg gttctccatc aaaaagacca tttttcataa atcttttcac
     7141 taatctgtct tctaacgaat gatatgaaat aacactcaat ctcccttccg gcttcaaaac
     7201 atctaatgct tgaactaaaa actctttcaa cacttccatt tcctgattca cttcgatacg
     7261 gattgcctga taaatttgtg ctaatatttt atgacttttg tgagccggta aaaaccttga
     7321 caatacttgt ttaagctgtt ccgtattctt tatgggagaa tcttttctgg catttacgat
     7381 ggtatttgca atcgctccgg cattggacaa ttctccaaac tcagtaaata ttttttttaa
     7441 tcgaacttct tcatattcat tgacaactgt aaatgcattt aagctatctt tttgattcat
     7501 tcgcatatcg agttccgcat caaatcgagt tgaaaaacca cgctcagcta catcaaattg
     7561 atgagatgaa acacctaaat ctgctaaaat tccatccact tctttcacac cataaaaacg
     7621 caaaaaccgc ttaatattcc tgaaattctc attaatcaat ttgaaacgtt catccggcaa
     7681 agcattggct aacgcatctt catcctgatc gaaggcaaac agttttccat tagacccaag
     7741 atgctttaaa atttctttag aatgccctcc tccaccaaaa gtcacatcga cataaacacc
     7801 atcaggctta atattcaaac cctctacgga tgcttgtaaa agaacaggat tatgatattc
     7861 cattgtcatc ttcatttaca ttccccataa catcttcagc caaatcagca aaatcaacag
     7921 ttgcatcatc aattgatttt tcatataaat ctttatccca aatctccaca atatttacag
     7981 ctgatgacaa cacaatatct ttcgatacag aagcaaaaac aaccaaatct tttggaacca
     8041 aaagccttcc caaagcatca atttcaacga tttttactcc agcagtaaac cttctgataa
     8101 aatcattatt ctttttcaca aaacgattaa gcttgttcac tttattcatc aaaacattcc
     8161 attccgccat gggatataat tctaaacaag gctgaaaaac agagcgtttc aatacaaaac
     8221 catcttgcaa tgaagaagcc aattgctttt ttaaaggagc cggcaaaaga agccttcctt
     8281 tagcatcaac tttacattca tatgttccta caatggtatt caacgggtta cggttttcat
     8341 ggtttgatag caaatataaa aagtttttac cacaatttac cacaaaatac cactttgtta
     8401 ataagtttta ccacttcgtt accccttaca tttaaaaatc atttgctaaa tttttacatt
     8461 ttatttaaca atccctttag aaatatcagg tttgcaataa aaaaggtaaa ggattaacaa
     8521 aagagacatg cttatatcct cttttttagt aaagtggatt gccattttta tttatacaaa
     8581 attgttgata attagaattt tagaaactcc tatttatttt ttgacagatc aacctaaatt
     8641 cctcgcaaat aaactttcgt atacttcttt tttatagata aaatcatata tttgtgagaa
     8701 atagaaagag gtcattttaa atggaacaac gttttaaaaa aga
//
LOCUS       DAOTCW010000095         8605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_5671_length_8605_cov_39.017310, whole genome shotgun sequence.
ACCESSION   DAOTCW010000095 DAOTCW010000000
VERSION     DAOTCW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8605
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5671_length_8605_cov_39.017310"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..3361
                     /gene="ccsA"
                     /locus_tag="P5335_08285"
     CDS             230..3361
                     /gene="ccsA"
                     /locus_tag="P5335_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006261727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRZ74913.1"
                     /translation="MDKKIYSFLFSTRLMACLFLLFAATMAAGTFIESYYDTDTARIW
                     IYNAWWFEVIMVFFVINFFGNIKRYQLWKKEKWATLLIHLSFIFILVGAFITRYISFE
                     GMMPIREGATESRFFSDKNYLTILTDGDYKGEPKRKTIEKPLLLSQVANNDFSITDDF
                     DAKPYSVTYKNYIMNATEIIKPDEKGETFLKLVESSNGTRHEHYLKSGEVQSLHGILF
                     AFNKATDGAINILEQGDGYFIKSTFGGDFMRMADQMKGLVVKDSLQPLMFRSLYNIAG
                     AQFVLPEAPIKGKKAWESNNDWKDKHTDDVLIVEVEANGKKEEVSLVGSKGKMSIPQN
                     VKLGDLDFTLIFGSKVYELPFSIKLNDFIGKRYPGTMNSFSAFESLVTVIDGSKKEDA
                     HIFMNNILDYKGYRFFQASFDQDEKGTVLSVNHDFWGTWITYIGYFLLYLAMMAILFD
                     KNTRFADIKRKLENVKKKKAALLSIAFFFFMSVGFAQDQNHQRPSLAEVEKFIDANIV
                     NKEHAAKFGRLVIQDDGGRMKPVNTFSSELLRKVSKSDHYRDYNSDQVFLSMSQTPNA
                     WFEVPLVYIKRGNDSIRKILNVDKDAKLVPFISFFDEKGNYKLTPYLDEAYKAAIPNQ
                     FQKDFIETDKKVVILNSALSGSILRIFPVPNDENNKWISYLELEQYQDTELKNLKNIL
                     PAYMQTLVEATEKNDYNFANEIVGGLEKFQIKYGSKVRPTDKKIDAEILYNKYDIFKR
                     LYYLYMIAGVMMLIFTVIQIFNSSKWVNYTVNTFHVFIGLLFALHTLGLIARWYISGH
                     APWSDAYESMIYVGWATMFFGLAFDRKSKLTVASAAFVASMILMIAHWNWMDPAIANL
                     QPVLNSYWLMIHVAVIVASYGPFTLGMILGIVSLLLILLTNDKNKERMNLNIQEITYI
                     NEMALTIGLVMLTIGNFLGGQWANESWGRYWGWDPKETWALISIMVYAFVIHARFVPS
                     LRGKWVFNLMSVFAFYSILMTYFGVNFYLTGLHSYASGDKVVTPTFVYVSVFLVSLLG
                     VLSFIKYKKYYKK"
     gene            3442..4029
                     /locus_tag="P5335_08290"
     CDS             3442..4029
                     /locus_tag="P5335_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009641512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRZ74914.1"
                     /translation="MKTNFIVLVACVMLMSCQNQAQNKKETANTTTEKVMKQSIHTFK
                     VQDIEGKEFDFATLKGKKIMVVNTASKCGLTPQYKDLQALYVEYKDKGLVIVGFPAND
                     FMKQEPGTNEEIGAFCQKNYGVTFPMMSKITVKGKEMHPIYQFLTQKAKNGLEDSEVE
                     WNFQKYLLNENGELEKVISPRTNPNDKEILEWLAS"
     gene            complement(4130..4627)
                     /locus_tag="P5335_08295"
     CDS             complement(4130..4627)
                     /locus_tag="P5335_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRZ74915.1"
                     /translation="MEILIRPYTESDVEAILAIVNYNILNSTSLYDYTPRTIEQQSNL
                     LKEKVAKNFPVLVAEKKGEVIGFGMYGEFRYREAYQFTVEHSVYVSKKYKGKGVGGKL
                     LKRLIAHAKTQKLHTMIGVIDAENKGSIQFHEKYGFKKVGVIKESGYKFNRWLDSVFM
                     QRILK"
     gene            4761..6422
                     /gene="menD"
                     /locus_tag="P5335_08300"
     CDS             4761..6422
                     /gene="menD"
                     /locus_tag="P5335_08300"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HRZ74916.1"
                     /translation="MIYPKIPLAQSIIEICHAKGIQHIVIAPGSRNAPLTIGFTANPY
                     FTCYSVADERCAAFFALGIAQQVKKPVVVICTSGSAVLNFYPAVAEAFYSQIPLVIIS
                     ADRPKEKVDIGDGQTIRQENVLANHTHFNANLTEEASEENDLKINEAFNKAFSKLGPT
                     HINAPFEEPLYETMDVSSVDTMISTFGKVYRNIPFEDIIASTNIWNTARKKLILIGTL
                     DPNELDPTIINHLANDPSVIVMTEATSNLHHETFLNHIDAIITPFNEEEVLAFQPEIV
                     LTFGGMIVSKRIKSLLRDYPPDNHWHVDRLRAYDTFGSLTFHFEANPSDFFHQFLPYT
                     KYIDSNYRQFGLQLQAVRKEKHEAYFADIPFCDFKAFETIVSLLPENIQLQVGNSSSI
                     RYLQLFDLPASIDVFCNRGTSGIDGSTSTAIGAAVANKKDTVLITGDVSFFYDSNALW
                     NNYIPKNFKIIVINNGGGGIFRILPGHQENEMFHTYFETAHCLTAESLANMYRFQYRK
                     ASDTASLQEEMKAFLEDSNEPQILEVFTPTYENDVFLKNYFKVLK"
     gene            6535..7494
                     /locus_tag="P5335_08305"
     CDS             6535..7494
                     /locus_tag="P5335_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRZ74917.1"
                     /translation="MSTIRRANTILGIKLCLLFIANNIYSQELAPRAYLWVPKNTTTL
                     VTGFLFSDGEILTDATLPVENVKAQIQGLTLGVSHTFAMMGMSSQLFVVAPYSWANVR
                     GDLNEEATEINRSGFSDMRVRLSVLFHNAPASTMKEIAQSPRKTILGASLNVVVPTGQ
                     YFSDKLINLGANRWAFFPELAVSHPISKKWLLDCYAGVWLFTDNNSFYPGESSRSQEP
                     LGAFQGHLSYNITPFFWVAINSTYYVGGNSAVNNVVKDDRASNSRIGITTVFPTGKLS
                     SLKLAVSTGAVVRIGQNFDTVSIGWQKSWIEGYKRTKKEKTKP"
     gene            complement(7538..>8605)
                     /locus_tag="P5335_08310"
     CDS             complement(7538..>8605)
                     /locus_tag="P5335_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ74918.1"
                     /translation="TVGTFIYTPLQIGVETDWVKIRAGHTHTVAIKNNGTLWAWGSNN
                     HSQLGDGTNVNRTTPIQIGTDTNWLSISPGYTHTLAIKTDGTLWGWGSNANGELGDGT
                     YTGKNAPVQIGTETNWVAVSTGQYHSKGIKADGTLWTWGRGIFGLHGDGTGNDNSGLN
                     KTSPLQIGTDTNWQAVSAGYETVTAIKTDGSLWVWGKNNYGQVGDGTFVTRNIPVQNT
                     TAVNCIAISQGMGFHDVAIQPNGQLWTWGLNIFGQLGNATNTNSNIPVNVDCGALSLD
                     ELGITKTEIKIYPNPVATMLYFETDHTVLNVEVYDIAGRLLISKTVEQNKINWNDLIT
                     GNYVLKVFTTGGIFTHRMIKE"
BASE COUNT         2667 a         1557 c         1623 g         2758 t
ORIGIN      
        1 tgaactaact caacctcatc actttgtaaa aaagcaacga ttaaatgttg agctacattt
       61 cccgaaccaa ttaatactac tgaaaccata gggcaaaaat aacaaaaata tatggctaat
      121 ttagaaatta aaacagacct taaatttgac ttaaaaaata aaataaaaca gatagtagtc
      181 ttacttttat aagtattttt gtgttttata aaaattattc tactctatta tggacaagaa
      241 aatatattcc tttctttttt caactcgttt aatggcttgc ttgtttttac tttttgcagc
      301 taccatggca gcaggaactt ttatcgaaag ctattacgat accgatactg cccgaatttg
      361 gatttataat gcgtggtggt ttgaagtaat catggttttc tttgtcatca actttttcgg
      421 aaatatcaaa cgctatcaac tttggaaaaa agaaaaatgg gctactttgt taattcactt
      481 gtcgttcata ttcattttag ttggagcatt cataacccgc tacattagtt ttgaaggcat
      541 gatgcccatt cgagaaggag ccacggaaag ccgttttttc tccgataaaa attacctaac
      601 cattttgacc gatggtgatt ataaaggaga accaaaaaga aaaaccattg aaaaaccctt
      661 gctactatcg caagttgcca ataatgactt ttcaattacg gatgattttg atgcaaaacc
      721 ctattcggtg acgtataaaa attacatcat gaacgctacc gaaatcatca aacccgatga
      781 aaaaggagaa acgtttttaa aattggtaga atccagcaat ggaacccgtc atgaacatta
      841 tttaaaatca ggtgaggttc aaagtttaca tggcatttta ttcgctttca acaaagcaac
      901 ggacggtgct attaatattt tagaacaggg tgatgggtat tttatcaaat caacttttgg
      961 aggtgatttt atgcgaatgg cagatcaaat gaaagggttg gttgtgaaag attctcttca
     1021 accgttgatg tttcgttctc tttacaacat tgcaggagca cagtttgttc ttcccgaagc
     1081 accaattaaa ggaaaaaagg cttgggaatc taacaatgat tggaaagata aacacaccga
     1141 tgatgttttg attgtagaag ttgaagccaa tggaaaaaaa gaggaagtta gcttagtcgg
     1201 ttcgaaagga aaaatgagca tacctcaaaa tgtgaagtta ggagatttag attttacctt
     1261 aatttttgga agcaaagtat atgaattacc gtttagcatc aaactcaatg attttatagg
     1321 aaagcgttac ccgggaacca tgaatagttt ttcggctttt gaaagtttag ttaccgtaat
     1381 tgatgggagc aaaaaagaag atgcacatat tttcatgaac aatattttag attacaaagg
     1441 ctatcgtttt tttcaagctt cgtttgacca agatgaaaaa ggaaccgtac tttctgtcaa
     1501 ccacgatttc tggggaacat ggataactta tataggttat tttttattat acctggcaat
     1561 gatggctatt ttgtttgata aaaacacccg tttcgcagat atcaaacgca aactggaaaa
     1621 cgtaaaaaag aaaaaagcag ctttactttc catagcattt ttctttttca tgagtgttgg
     1681 ttttgctcag gatcaaaatc accaacgacc ttccttggca gaagtggaaa aattcattga
     1741 tgcaaatatc gtcaacaaag aacatgctgc taaattcggt cgattagtga ttcaggatga
     1801 tggcggacga atgaaaccgg tcaacacttt ttcctctgaa ttgttgcgga aagtaagtaa
     1861 aagtgaccat tatagagact acaattcaga ccaagtattt ttatcgatgt cacaaacgcc
     1921 aaatgcttgg tttgaagtgc cattggttta tatcaaacga ggaaacgaca gcattcgcaa
     1981 aatattaaac gttgacaaag acgctaaatt agttccgttc atcagctttt ttgatgaaaa
     2041 aggcaactac aagttaacac cttatttaga tgaagcctat aaagcggcca ttcctaatca
     2101 atttcaaaaa gattttatag aaaccgataa aaaggttgtt attttaaatt ccgctttatc
     2161 cggaagtatt ttacgcattt tccccgtgcc gaatgatgaa aataataaat ggatttctta
     2221 cctcgaatta gaacaatacc aagataccga actgaaaaac cttaaaaaca ttcttccggc
     2281 ctacatgcaa acattagtgg aagcaacgga aaagaatgat tataacttcg ccaatgaaat
     2341 tgttggtgga ttagagaaat tccaaataaa atacggaagc aaagttcgtc caaccgataa
     2401 aaaaatcgac gcagaaatac tttataataa atacgatatt ttcaaacggt tgtattattt
     2461 atacatgata gccggtgtaa tgatgttgat tttcacggtc attcaaattt tcaattcctc
     2521 taaatgggtg aattacaccg taaatacatt ccatgttttt attggtctgc tgtttgcttt
     2581 acatacactc ggtttaattg ctcgttggta tatttccggt catgctcctt ggagtgatgc
     2641 ctatgaaagt atgatttatg tgggctgggc aaccatgttc ttcggtttag cgtttgacag
     2701 aaaatctaaa ctaactgtag cttctgcagc ttttgtcgct tccatgattt taatgattgc
     2761 tcattggaat tggatggatc ccgccattgc aaacttacag ccggttttaa attcctattg
     2821 gttaatgatt cacgtagccg tcattgtagc tagttatgga ccattcactt tgggaatgat
     2881 tctagggatt gtttccctgt tattgattct tttgaccaat gacaaaaaca aagaacgaat
     2941 gaacttaaac attcaggaaa tcacttatat caacgaaatg gcgttaacca ttggattagt
     3001 gatgctcacc atcggtaact ttttaggagg tcaatgggcc aatgaaagtt ggggtcgtta
     3061 ttggggttgg gatccaaaag aaacatgggc tttaattagt atcatggtct atgcctttgt
     3121 gattcatgct cgatttgtgc cttccttacg cggtaaatgg gtgtttaatt taatgagtgt
     3181 ttttgctttc tattctattt taatgaccta tttcggggtt aatttctacc ttaccggttt
     3241 acattcctat gccagtggtg ataaagtagt tacaccaaca tttgtttacg tatccgtatt
     3301 tttagtgtct ttgttaggcg tattgtcttt tatcaaatac aagaagtatt ataaaaaata
     3361 gccttttttt aaaaccaaat tatgatttgt ttaggtcggc tatcatcaga aagtttgtaa
     3421 atttgaaata aaaagaaacg tatgaaaaca aattttatag tattagtagc ttgtgtgatg
     3481 ttaatgagtt gtcaaaacca agctcaaaac aaaaaagaaa cggcaaacac aacaacagaa
     3541 aaagttatga aacaatccat ccacacgttt aaagtgcaag acatagaagg gaaagagttt
     3601 gattttgcca ctctaaaagg aaaaaaaata atggtagtca ataccgcttc taaatgtgga
     3661 ttaacaccac aatacaaaga tttacaggca ttatatgtag aatataaaga caaaggatta
     3721 gtgattgttg ggtttcctgc gaatgatttc atgaaacaag aaccgggaac gaatgaagaa
     3781 attggtgctt tttgtcaaaa gaactacgga gtaacattcc caatgatgag taaaataaca
     3841 gtgaaaggaa aagaaatgca ccccatttat caattcctga ctcaaaaagc taaaaatggt
     3901 ttagaagaca gtgaggttga atggaatttt caaaaatacc ttctaaacga aaatggtgaa
     3961 ttagaaaaag taatcagccc aagaacaaat ccgaatgata aggaaatttt agagtggttg
     4021 gcgagttaat tttcctcctc tttttaaagc ttagtacata ggaacacaga tttaagaaat
     4081 caaataaatt tttaaaattt tactaatttc ttaaatctgt gttctaattt tattttaaaa
     4141 ttcgttgcat aaaaacagaa tctaaccagc ggttaaattt ataccctgat tctttgataa
     4201 cgccaacttt tttaaagcca tatttttcgt gaaactgaat gcttcctttg ttctccgcat
     4261 caattacacc aatcattgta tgtagttttt gtgtttttgc atgagcaatt aaacgtttca
     4321 ataattttcc gccaacacct tttcctttat attttttgga gacataaaca gaatgttcaa
     4381 ctgtaaattg ataggcttcc cgatagcgaa attcaccata catgccaaaa ccgataactt
     4441 cgcctttttt ttcagccaca agaacaggaa agttttttgc cactttttct tttaaaagat
     4501 tactttgttg ttcgattgtt cttggtgtgt aatcatataa agaagtagaa ttcaagatat
     4561 tatagttaac gatggctaaa atcgcttcta cgtcggattc agtataaggt ctaatgagta
     4621 tttccatatt gttgaaaagt tgttcaaaga taacaaaact ttaaaggttt ataaaccttg
     4681 ttattgttat ctaaagtata aatttgtgta acctaaattc taacataaaa cctaaggcac
     4741 aaaatttttt aatgtaaaga atgatttatc ctaaaatacc gcttgctcaa agcatcatcg
     4801 aaatttgtca tgccaaaggt attcagcata ttgttatcgc accgggttcg cgtaatgctc
     4861 ctttaacgat tgggtttact gctaatccgt attttacatg ttatagtgtg gctgacgaac
     4921 gttgtgctgc attttttgcg ttagggattg ctcaacaagt taaaaaacct gttgtggtta
     4981 tctgtacatc cggttctgcc gtgcttaatt tttatccggc agtggctgaa gctttttata
     5041 gtcagattcc ccttgtcatt atttcggcag acagaccaaa ggaaaaagtt gatattggtg
     5101 acggtcaaac cattcgtcag gaaaatgtat tggccaatca tacccatttc aatgcgaatt
     5161 taaccgaaga agcatcggaa gaaaacgacc ttaaaatcaa cgaagctttt aacaaagcct
     5221 tttctaaatt agggccaacc cacataaacg ctcctttcga agaaccatta tacgaaacca
     5281 tggacgtgtc ttccgtagat accatgattt ctacttttgg gaaagtatac cgcaatattc
     5341 ccttcgaaga cattattgct tccacaaaca tttggaatac cgcccgaaaa aagttaatcc
     5401 tcattggaac actcgaccca aatgaattag accctacaat catcaaccat ctggcgaacg
     5461 acccctcggt gattgtcatg acggaagcta cctctaatct gcatcatgaa acttttttaa
     5521 accatatcga tgcaatcatt acgcctttta atgaggaaga agtactggct tttcaaccgg
     5581 aaattgtttt gacttttgga ggtatgattg tttcgaaacg catcaaaagt ttgttgcgtg
     5641 attatccacc tgataaccat tggcatgtgg atcgacttcg ggcttatgat accttcggta
     5701 gtcttacttt tcattttgaa gcaaacccaa gtgatttttt ccatcagttt ttgccttaca
     5761 ccaaatatat cgacagtaat tatcgtcaat ttggcttaca gcttcaagcg gtacgcaagg
     5821 aaaaacacga ggcttatttt gctgatattc cgttttgcga tttcaaagcg tttgaaacca
     5881 ttgtttctct tttgcctgaa aatattcagt tgcaagtggg gaatagttca tccattcgct
     5941 atttgcaatt gtttgactta ccggcttcca tcgatgtttt ttgtaatcgg ggaaccagcg
     6001 gaattgacgg aagcacatct accgccatcg gggctgcagt ggccaataaa aaagacactg
     6061 ttttaataac gggcgatgta agtttctttt atgacagcaa tgccttatgg aacaactaca
     6121 ttcctaaaaa cttcaaaatc attgtcatca acaatggagg aggaggtatt ttccgcatct
     6181 tacccggtca tcaggaaaat gaaatgttcc acacgtattt tgaaacagca cattgcttaa
     6241 cagccgaatc gttagcgaac atgtatcgtt ttcaatacag aaaagcaagt gataccgctt
     6301 ctttgcaaga ggaaatgaaa gcgtttttag aagattccaa tgagcctcaa atcttagaag
     6361 ttttcacgcc aacgtatgaa aacgatgtct ttttaaagaa ctattttaaa gtacttaaat
     6421 aattcttttt gtttttcagg tatttagatt tgtaatatct ttattaaact attattgtta
     6481 acttgacttt tataatcaac ccttattaaa aaaattaaaa gaaactatcc tgcaatgagt
     6541 accattcgaa gagcaaacac tattttagga attaaacttt gcttgttgtt cattgcaaat
     6601 aatatatatt cccaagaatt agcacctaga gcctaccttt gggttccaaa aaacaccaca
     6661 accttagtaa ccggattttt attttctgat ggtgaaatcc tgactgatgc caccttgccg
     6721 gtggagaatg ttaaagctca aattcaagga ttaaccttag gcgtttcgca cacgtttgct
     6781 atgatgggaa tgtcttcgca gctttttgta gtggctcctt attcatgggc aaacgttagg
     6841 ggtgatttaa acgaagaggc aacggaaatc aatcgctcgg ggttttcgga tatgagagtt
     6901 cgcttatcgg ttttgtttca caatgccccg gcaagcacca tgaaagaaat tgctcaatcg
     6961 ccccgaaaaa caattttagg agctagtttg aatgtggtag ttcccacggg gcagtatttt
     7021 tcagataagt taattaacct tggagcaaat cgttgggctt tttttccaga gctggctgtt
     7081 tctcatccca tcagtaaaaa atggttgctg gattgctatg ccggggtttg gctttttacg
     7141 gataacaaca gtttctatcc cggagaatct agccgttcgc aagaaccatt aggagctttt
     7201 caaggccatt tgagttataa tattactccg tttttttggg tagctatcaa ttcaacttat
     7261 tatgttggtg gaaattctgc agtaaataat gtcgttaaag acgacagagc atctaattct
     7321 cgaattggta taacaacggt attcccaacg ggaaagttaa gttcgttgaa acttgccgtt
     7381 agcacggggg cagttgttcg gattggtcaa aattttgata ccgtttccat cggttggcaa
     7441 aaatcatgga ttgaaggata taaaagaact aaaaaggaaa aaacaaaacc ctaagcttcc
     7501 attacatcga atgccctttt atttttaggg ctgataacta ttcttttatc atgcggtggg
     7561 tgaatatccc gccagtagtg aataccttca aaacataatt tccggtaatc aagtcattcc
     7621 aatttatttt attttgctca acggtttttg aaatcaaaag tcgtccggcg atgtcgtata
     7681 cttcaacgtt taacacggta tggtctgttt caaaatataa cattgttgcg accggatttg
     7741 ggtaaatttt tatttccgtt ttggttattc caagttcatc taatgacaaa gctccacaat
     7801 ctacgttgac cggaatgttg ctgttggtat tggttgcgtt acccagttgc ccgaagatgt
     7861 ttaaacccca cgtccagagt tgcccgttgg gttgaatggc tacatcgtga aatcccattc
     7921 cttgtgaaat ggcgatacag ttcacagcgg ttgtgttttg aacgggtata tttcgtgtaa
     7981 caaaggttcc gtcacctact tgaccgtaat tgttttttcc ccaaacccac aaactcccgt
     8041 ctgtttttat ggcggtaacg gtttcatagc cagctgaaac ggcttgccag tttgtatctg
     8101 ttccgatttg aaggggcgat gttttgttta atcccgaatt gtcattgcct gttccgtcgc
     8161 cgtggagtcc gaatattcct cggccccacg tccatagggt tccgtctgct ttgatgcctt
     8221 ttgaatgata ttgaccggtt gacactgcta cccaattggt ttcggttcct atttgcacgg
     8281 gagcattttt tccggtatac gtaccgtcac ccagttcgcc gtttgcattg ctgccccaac
     8341 cccaaagcgt tccatctgtt ttgatggcta acgtatgggt gtagccgggc gaaatgctta
     8401 accaatttgt gtctgtgcct atttgtattg gtgttgttct gttcacattg gttccatctc
     8461 ctaactgcga atggttatta ctaccccaag cccacaaggt gccgttgttt tttatggcta
     8521 cggtgtgcgt gtggccggcc ctaattttta cccaatcggt ttctactcca atttgtaggg
     8581 gagtgtagat aaacgtacct acggt
//
LOCUS       DAOTCW010000096         8457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_5825_length_8457_cov_36.317900, whole genome shotgun sequence.
ACCESSION   DAOTCW010000096 DAOTCW010000000
VERSION     DAOTCW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5825_length_8457_cov_36.317900"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1755)
                     /gene="recN"
                     /locus_tag="P5335_08315"
     CDS             complement(103..1755)
                     /gene="recN"
                     /locus_tag="P5335_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRZ74919.1"
                     /translation="MLTSLSINNYALIDKLSIDFSNGFSTITGETGAGKSILLGALGL
                     VLGKRADLTSLKNKEEKCIIEGHFSIKKYALKDFFELNDLDYEDVTIIRREILPSGKS
                     RAFVNDSPVNLQTLVELSEYLLDIHSQHQTLELTEEQFQFQIIDAVASNQSLLVDYRH
                     NLKNYKSLLDTLEQQKELYAEGLKEQDYLAFLYNELEQAKLSDGEQEEMEIQLEKLNN
                     VELIKEQLEKSLLLSDNEQFGILQQIKEFKIAIQKISQFSSEYALLYERIESISIELK
                     DIVNEVNRFSDSVDNDPERLAFITQKLQLIYTLQKKHQVNTVGELLQIQQELNGKVSS
                     LETLEKSILELENQIQKEQIILIDLAKNIHLNRKNSLPSLTKTLISILSQLGMPNVQF
                     NIELTFSDTFFSNGKDTIQFLISTNKGSDFGLLKKVASGGEMSRIMLAVKAVLAQYSS
                     LPTLIFDEIDTGVSGEIANKMGDIMKEMSKNMQIFAITHLPQIAAKGNFHYKVFKSDL
                     GDKTKSEIILLNNEERIIEIAQILSGNSLSDSAINHAKALLN"
     gene            complement(1845..>2533)
                     /locus_tag="P5335_08320"
     CDS             complement(1845..>2533)
                     /locus_tag="P5335_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HRZ74920.1"
                     /translation="ECSMFINVSEVDNTTFTATIQIQSSRPVYNSIYSTPIFNYNDKD
                     FTFKYVEFESLNFNPNSFDSNLVSVVAFYSYIIMALDADSFESLKGTRYLEMAQNVMS
                     VAQTGGYKGWSQTDGLQNRYYLISDLLSNTFNPIRQAYFEYHANGLDKMADDVKLGKE
                     NVKTALLNLSVIHSVRPNAFLTRVFFDAKSDEIADIFSDGPSIKTTELVDNLNRLSPL
                     NASKWSTIKF"
     assembly_gap    2534..2633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2634..2887)
                     /locus_tag="P5335_08325"
     CDS             complement(<2634..2887)
                     /locus_tag="P5335_08325"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HRZ74921.1"
                     /translation="MRKIIVVLVLMLFGFIQAQELNCVVSVNSERVTANTQQVFKTLE
                     TSLNDFVNKTKWSNQSFNQNERIECSMFINVSEVDNTTFTA"
     gene            complement(2880..4103)
                     /gene="coaBC"
                     /locus_tag="P5335_08330"
     CDS             complement(2880..4103)
                     /gene="coaBC"
                     /locus_tag="P5335_08330"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRZ74922.1"
                     /translation="MSVLKGKKVLLGVTGGIAAYKTASLVRLFIKAGAEVQVIMTPAS
                     KEFVTPLTLSTLSKNPVFSEFYNDENESDNQNATWNNHVELALWADLMLVAPTTANTL
                     AKMANGNCDNLLMATYLSAKCSVYFAPAMDLDMYKHPTTIANLNKLSLFGNIMIPAEL
                     GELASGLSGEGRMAEPENIIAFLENHLLQQLPLKGKKILITAGPTYEAIDPVRFIGNH
                     STGKMGFDIANEAAQLGAEVILVSGPSHFQNDNSSIQLIRVTSSDEMFEQCHLFFDDV
                     DVAIAAAAVADYKPKQIAKQKIKKTEAAFSIELEKTKDILASLGKIKTKQFLVGFALE
                     TENEIEHAKLKIQKKNLDLIVLNSLNDEGAGFGKATNKITFIDKSFKIEPMPLKPKEE
                     VAKDILKKIIGLYHA"
     gene            complement(4110..4427)
                     /locus_tag="P5335_08335"
     CDS             complement(4110..4427)
                     /locus_tag="P5335_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRZ74923.1"
                     /translation="MDLKKTNAPVNTITYDKRVIEEPTGNVYEAITIMAKRANQINSE
                     IKKELIEKLEEFATYNDSLEEIFENKEQIEVSKFYEKLPKPHALAVQEWLEGKIYYRD
                     TTK"
     assembly_gap    4527..4626
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4627..5386)
                     /gene="bamD"
                     /locus_tag="P5335_08340"
     CDS             complement(<4627..5386)
                     /gene="bamD"
                     /locus_tag="P5335_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRZ74924.1"
                     /translation="MNKLYFIFIFALIVTSCGPYQAAMKSEDVGVKYTMAESKYNEGK
                     YEKAIRLFDQIAPAYRGKPQAEKLFYMYAQSYFLTKQYYLAGYQFESFVSSYPKSEKV
                     EECAYLGAYCYSKLSPVYSLDQVDTYKAIDKMQGFIDNYPESVLLAEANVIVKQLREK
                     IEKKAFENAKQYNTIYDYKSAIVALDNFISDYPGTPFKEAALYYKFDSAYYLAINSVS
                     EKVEERLKSAKDAYDKYIKHSNSSEFKEQADDLMN"
     gene            complement(5557..6066)
                     /locus_tag="P5335_08345"
     CDS             complement(5557..6066)
                     /locus_tag="P5335_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRZ74925.1"
                     /translation="MLSSKVLEALNKQIKMEGDSSQVYLAMASWAEVQGFEGISTFMY
                     KQSDEERMHMLKLIKYVNQRGGEATVGSTLEPKLDVTNYKALFKQLLEHEIKVSESIN
                     NLVGITLDERDFATHNFLQWYVAEQIEEEATARYILDKINLIGDDKGGLYLFDNEMKN
                     FVSPMAPAK"
     gene            complement(6100..>6667)
                     /locus_tag="P5335_08350"
     CDS             complement(6100..>6667)
                     /locus_tag="P5335_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRZ74926.1"
                     /translation="SPYYNKPTQEGIYQHFKAIAEASPVPVILYNVPGRTASNMAPAT
                     VFRLAHDFKNIVAIKEAAGDIVQAMKLIQNKPNDFLVISGDDMITLPMVLAGGAGVIS
                     VIGEGFPKEFSTMVRFGLERNVEEAYQLHYLVADAIDLIFEQGNPAGIKEVFKTLGLC
                     ENTVRLPLVNVNEDLANRIRQMVKKVLN"
     assembly_gap    6668..6767
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6768..7136)
                     /locus_tag="P5335_08355"
     CDS             complement(<6768..7136)
                     /locus_tag="P5335_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRZ74927.1"
                     /translation="MQSLIGTGVALVTPFKNDFSVDTQALTRIVNFQIEGGIDYLVVL
                     GTTAESATLNEEEKNLVIETVIKANKNRLPLVLGVGGNNTYKVVEELKTRDLSPFVAV
                     LSVSPYYNKPTQEGIYQHFKA"
     gene            complement(7138..7656)
                     /locus_tag="P5335_08360"
     CDS             complement(7138..7656)
                     /locus_tag="P5335_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74928.1"
                     /translation="MFLKFFKNFSAKRIVKKSSLNVNSTISDKPIETVGLLIDETFFT
                     EKDKLIQQLVSNGIEVKNLTVLAFKNSYKKKEIIDYPHYSKKDVSWLGTIEKKEALDF
                     IAKDYDLLISYYDVKKTPLVIVTHQSKANFKVGFTSVDKRLNHFMIETQVEKYSVFTD
                     ELFKYLQILKKI"
     gene            7706..>8457
                     /locus_tag="P5335_08365"
     CDS             7706..>8457
                     /locus_tag="P5335_08365"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRZ74929.1"
                     /translation="MLKLKNYIVVMKHFVLIITFSLLLFGCKTSYEIVKIEGKQIGVS
                     DPTAEQPELENFIKPYREHINKDLDSVLAFCPETLDKSKGEWQTNIGNFMADVCLSGS
                     NKIFSKRYNKSIDLCLLNHGGIRSIIPKGNVTTRTAFEVMPFENNMVVIALKGSQINE
                     LVAHIIKEKKPHPLSGMKLILDKNLLVKSIMIQDKEIDLNTIYYVATSDYLANGGDNM
                     RFFLTNEGVFDLDYKIRNILIDYFKEVNTVEAS"
BASE COUNT         2661 a         1382 c         1137 g         2977 t
ORIGIN      
        1 ttcatctaat gctccgaata taattcccct ttttcctttt agtaaattat acataccttt
       61 tttaatttta atttatatga cgtttcaaat atagttaaaa aatcagttta acaatgcttt
      121 tgcgtgattt atggctgaat cagataaaga atttcctgaa agtatttgag caatttcgat
      181 gattctttct tcattattta acaatataat ttcagatttt gtcttgtctc ccaaatcaga
      241 tttaaaaact ttgtaatgaa aattaccttt ggcagcaatt tgtggcaaat gagtgatggc
      301 aaaaatttgc atattcttac tcatttcctt cataatatct cccattttat tagcaatctc
      361 accggaaaca ccggtatcta tttcatcaaa aattagggtt ggcaaacttg aatattgagc
      421 taaaacagct ttaacagcta acataattcg agacatttca cctccggaag ctactttttt
      481 cagtagtcca aaatcagatc ctttgttggt cgaaatcaaa aactgaatag tatcttttcc
      541 gtttgaaaaa aaggtgtccg aaaatgtcaa ttcaatatta aattgcacat ttggcatgcc
      601 tagctgactc aaaatagaaa taagcgtttt tgtcaaactt ggtaggctgt tttttctgtt
      661 aaggtgaata tttttagcta aatcaatcaa aattatttgc tccttctgaa tttgattttc
      721 aagttccaaa attgattttt ctaaagtttc caaagaactc acttttccat tcaattcttg
      781 ttgaatttga agcaattcac caacggtatt aacctgatgt tttttctgta aagtgtaaat
      841 taattgtagt ttttgggtga taaaagctaa tctctccgga tcattatcaa cggaatcaga
      901 aaaacgattt acttcattta caatgtcttt cagttcaatt gaaatactct caatacgctc
      961 ataaagcaaa gcgtattctg acgaaaattg actaattttt tggatggcaa ttttaaactc
     1021 ttttatttgc tgaagaatac caaactgttc attatcagac aataacaaac ttttttctaa
     1081 ttgttcttta attaattcaa cgttattcaa tttttccagt tgaatttcca tttcttcttg
     1141 ttcaccatca cttaatttag cttgttccaa ttcattatac aaaaaggcta aataatcttg
     1201 ctcttttaaa ccttctgcat ataattcctt ttgttgttct aatgtatcta aaagtgattt
     1261 gtaatttttc aagttatggc gataatcaac taataaactc tgatttgagg ccacagcatc
     1321 aattatttga aactgaaatt gctcttctgt taattccaga gtttgatgtt gcgaatgaat
     1381 atccaataaa tactcgctga gttcaaccaa ggtttgcaaa ttaactggac tgtcattaac
     1441 aaaagctctt gattttccgg agggaagaat ttcacgtcga ataatagtta catcttcata
     1501 atccaaatcg ttaagttcga aaaaatcttt taaagcatat tttttaattg aaaaatgtcc
     1561 ttcaattata catttctctt ctttattttt aagcgaagtt aagtctgctc tttttcctaa
     1621 aactaatcca agtgctccta atagaattga ctttcctgct ccggtttcac cggtaatggt
     1681 tgaaaatcca tttgaaaaat caatggataa tttgtcaatt aaagcataat tatttatgga
     1741 taatgaagtt agcataacta ttctgaatac aaataaacaa ttgcaatgta ctcaattaat
     1801 taaaagaaga ttgaagtaaa aatcagatta tttaaacaaa gttattaaaa ctttatcgtt
     1861 gaccatttag atgcatttaa aggagagagt cgatttaaat tatcaactaa ttcagtagtt
     1921 tttatgcttg gcccatctga aaaaatatcg gctatttcat ctgactttgc atcaaaaaac
     1981 actcgggtta aaaatgcatt tggtctaact gaatgaatta ctgatagatt taaaagtgcg
     2041 gttttcacat tctcttttcc taatttcaca tcatctgcca ttttatcaag accattcgca
     2101 tggtattcaa aataggcttg tctgattgga ttaaacgtat tagacaacaa atcactgatt
     2161 aaataatagc ggttttgtaa accatctgtt tgagaccaac ctttataacc tcctgtttga
     2221 gctacactca tcacgttttg agccatctct aaatacctgg ttccttttaa agattcaaat
     2281 gaatctgcat ctaaggccat tataatgtaa ctatagaagg caaccactga aactaagtta
     2341 gagtcaaatg aattgggatt aaaatttaaa ctttcaaatt caacatattt gaaagtaaaa
     2401 tctttatcgt tataattaaa tattggggtt gaatatatcg agttataaac cggtctggaa
     2461 gattgaattt gaatagttgc cgtaaaagtg gtattatcta cttctgaaac attaataaac
     2521 atggaacatt caannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgtaa
     2641 aagtggtatt atctacttct gaaacattaa taaacatgga acattcaatt cgttcatttt
     2701 gattaaaact ttgattagac cattttgttt tattaacaaa atcatttaat gatgtttcta
     2761 acgtcttgaa tacctgttgt gtatttgccg taacccgttc agaatttaca cttacaacac
     2821 aattaagttc ttgagcttga ataaatccaa acagcattaa aactaaaaca acgattattt
     2881 tacgcatgat acaaccctat tatttttttt aaaatatctt tagccacttc ttcctttggt
     2941 tttaatggca tcggttcaat tttaaatgat ttgtcaataa atgtaatttt gtttgtcgct
     3001 ttaccaaaac cggcaccttc atcattaagc gaatttaaaa caatcaaatc taagtttttt
     3061 ttctgaatct tcaacttggc atgttcaatt tcattttcag tttctaatgc aaaacctact
     3121 aaaaattgtt tcgttttaat tttccctaag gaagctaaaa tatctttcgt tttttcaagt
     3181 tcaatagaaa atgctgcttc tgtttttttt attttttgtt tagctatttg ttttggttta
     3241 taatccgcca cagccgctgc tgctattgca acatcaacat cgtcaaaaaa cagatggcat
     3301 tgttcaaaca tttcatctga agatgtcacg cgaattaact gtatacttga attatcattt
     3361 tgaaaatgac ttggtccgga cactaaaata acttcagctc ccaattgtgc agcttcattg
     3421 gcaatatcaa aacccatttt tccagttgaa tgatttccta taaaacgcac aggatctatc
     3481 gcttcgtatg tgggacctgc agttattaat attttttttc cttttaaagg taattgttgt
     3541 agcaaatgat tttctaaaaa ggcaataata ttttcaggct ctgccattct gccttcaccg
     3601 gataagccac tagctaattc accgagctca gcaggaatca taatgtttcc aaaaagcgat
     3661 aatttattta aattggctat tgtagttgga tgcttataca tatctaaatc cattgccggg
     3721 gcaaaatata ccgaacattt tgcggacaaa taggtagcca tcaataaatt atcacaattc
     3781 ccattagcca tttttgctaa ggtattcgcc gttgtaggag ctactaacat caaatccgcc
     3841 caaagggcta attcaacgtg attattccaa gtagcatttt gattgtccga ttcattttcg
     3901 tcattataaa actcagaaaa tacaggattt ttagaaagtg tagaaagagt taatggtgtc
     3961 acaaactctt tagaagcagg tgtcataatg acttgtacct cggcacctgc ttttataaat
     4021 aatcgaacta aggaagcagt tttataggca gcaattccac cggttacacc cagtagtact
     4081 tttttccctt ttaaaactga cataacgttt tattttgtag tatctctgta gtaaattttt
     4141 ccctctaacc actcttgaac agctaacgca tgtggtttag gtaatttttc ataaaatttt
     4201 gaaacttcaa tttgttcttt gttttcaaaa atttcttcta aactatcatt ataggtagca
     4261 aactcttcta atttttcaat taattccttt ttaatttcag aattaatttg atttgctctt
     4321 ttagccataa tagtaatagc ttcgtaaaca ttccctgtag gttcttcaat tactctttta
     4381 tcatacgtaa ttgtgtttac cggagcatta gtttttttta aatccatgac atgtatctat
     4441 ttagaaaatt gttgtaaatt ttgatttagt ttattcatta aatcatctgc ttgttcttta
     4501 aattctgatg aattagaatg cttaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnntatt cattaaatca tctgcttgtt ctttaaattc tgatgaatta gaatgcttaa
     4681 tgtatttatc gtaagcatct tttgcacttt ttaatcgttc ttctaccttc tctgaaacgc
     4741 tattgattgc taaataataa gcagaatcaa atttataata caacgcagct tctttaaatg
     4801 gtgtacccgg ataatctgaa ataaaattat ccaaagctac gatagctgat ttgtaatcat
     4861 aaatggtatt atattgtttt gcgttttcaa aagccttctt ttcaattttt tctcttaact
     4921 gttttacaat aacgttagct tcagcaagta gaactgattc tgggtaatta tcaataaaac
     4981 cctgcatttt atcgattgct ttgtaggtat ccacttgatc caatgaatag acaggtgata
     5041 atttagaata acaataagct cctaaatacg cacattcttc aactttttca ctttttgggt
     5101 aagaagatac aaaactttca aattgataac cggccaaata atattgcttt gttaaaaaat
     5161 aactttgagc atacatgtaa aacaactttt cggcttgtgg tttgcctcta taagcgggag
     5221 cgatttgatc aaataaacga atcgcttttt catattttcc ttcattatat tttgattcgg
     5281 ccattgtata tttaacgcca acatcttcgc ttttcatagc agcttgatat ggtccacaag
     5341 aagtgacgat taaagcaaaa atgaaaataa aatacaattt attcattaga tgggttttaa
     5401 gggcagattc taaagtaaaa cttacttgaa aaatcgaact gcaaatttag ttattaattg
     5461 cagactacaa aacatttttt tcatagtctt ttaaaggtgt aaaaaaacaa aaagtagcct
     5521 aaattaaagc tacttttata tttagattaa cataaattat tttgcaggtg ccatcggact
     5581 cacaaaattt ttcatttcgt tgtcaaaaag atacaaaccg cctttgtcgt caccaattaa
     5641 attgatttta tccaagatgt aacgggctgt tgcttcttcc tcaatttgct ctgcaacata
     5701 ccattgaaga aaattatgtg ttgcgaaatc tctttcatcc agtgtaattc caactaaatt
     5761 attaatactt tcagatactt taatttcgtg ttctaaaagt tgtttaaaca aagctttgta
     5821 attggttaca tctaattttg gttctaaagt tgaaccaact gtagcttcac cccctctttg
     5881 atttacatat ttgattaatt tcaacatatg cattcgttct tcgtcagatt gcttatacat
     5941 aaatgttgaa ataccttcaa atccctgaac ttccgcccaa gatgccattg ctaaataaac
     6001 ttgagaagaa tcaccttcca ttttaatttg cttatttaat gcttctaata cttttgatga
     6061 taacataata tttcttttgg ttattatatt ttgtataatt taatttaaaa cttttttcac
     6121 catttgtcgg attctatttg ctaagtcttc atttacatta accaaaggaa gccttactgt
     6181 attttcgcat aatcctaaag ttttgaaaac ctctttaatt ccggcaggat taccttgctc
     6241 aaatatcaaa tcaattgcat cggcaacgag ataatgaagt tgataagctt cctccacatt
     6301 tctttctaaa ccaaatctga ccattgttga aaattctttt ggaaatcctt ctcctattac
     6361 agaaataact cctgcacctc cggctaatac cattggcaag gtaatcatat cgtctcccga
     6421 tataaccaaa aaatcattag gtttattttg gattaatttc atagcttgaa caatatcgcc
     6481 cgctgcttct ttaatagcaa caatattttt aaaatcatga gctaatctaa aaacagttgc
     6541 cggagccata ttgcttgctg ttcttccggg aacattgtat aaaataacag gaacaggaga
     6601 agcttctgca atcgctttaa aatgctgata aattccttcc tgagttggct tattataata
     6661 tggagaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc tttaaaatgc
     6781 tgataaattc cttcctgagt tggcttatta taatatggag aaaccgacaa aaccgccaca
     6841 aaaggtgata aatcacgcgt ttttaactct tcgactactt tgtaagtatt atttcctcca
     6901 actcctaaaa ccaaaggcaa tctgttttta ttggctttga ttacagtttc aataactaaa
     6961 tttttttcct cctcatttaa tgttgcactt tctgctgttg ttcctaaaac aactaggtag
     7021 tctatacctc cttcaatttg aaaatttact attcgggtta acgcttgtgt gtcaacagaa
     7081 aaatcatttt tgaatggtgt aaccaaggcc acacccgttc caattagaga ttgcatatta
     7141 tatttttttt agtatttgta aatatttaaa tagttcgtct gtaaaaacac tatatttttc
     7201 aacttgagtt tcaatcataa aatgattcaa acgtttatct acagaggtga atccaacttt
     7261 aaaattggcc ttagactgat gcgtcactat cactaaagga gtttttttaa catcataata
     7321 actaatcaat aaatcataat ctttcgcaat aaaatcaagt gcctccttct tttcgatcgt
     7381 ccctaaccat gaaacatctt ttttgctgta gtgagggtaa tctattattt ctttcttttt
     7441 ataactgttt ttaaaagcca atacggtaag attttttact tcaattccat ttgaaactaa
     7501 ttgttgaatc aatttatctt tttctgtaaa aaatgtttcg tctatcagca aacctactgt
     7561 ttcaattggc ttatcagaaa tagttgaatt aacattgagt gaactttttt ttactattct
     7621 ttttgcggaa aaattcttga aaaattttaa aaacattgta cctttacttg gtttacaaaa
     7681 ttactgaatt aaagttgttt tttcgatgct aaaactaaaa aactatatcg ttgtaatgaa
     7741 acattttgtg ttaattataa cattttcttt actattattc ggatgcaaaa catcctatga
     7801 aattgtaaaa attgaaggca aacaaattgg agtttctgac ccaacagctg aacaacctga
     7861 attagaaaac tttataaaac cttatcgaga acatatcaac aaagatttag acagtgtttt
     7921 agctttttgc ccggaaacat tagacaaaag caaaggggaa tggcaaacca atataggaaa
     7981 tttcatggct gatgtttgtt tatcaggaag caacaaaatt ttttcaaaac gctacaataa
     8041 atcaatcgac ctttgtttat taaatcatgg tggaattcgc tctattattc caaaaggaaa
     8101 tgttaccacc cgaactgctt ttgaggtcat gccttttgaa aataatatgg tggtaattgc
     8161 cttgaaagga agtcaaatta atgaacttgt tgcccatatt attaaagaga aaaaacctca
     8221 tccgttaagt ggaatgaaat taattctaga caaaaatcta cttgtaaaat ccattatgat
     8281 tcaagataaa gaaatagatt taaatactat ttattacgta gctacttccg attatttagc
     8341 caatggaggt gataatatgc gatttttttt aaccaatgaa ggagtctttg atttagatta
     8401 taaaattaga aatatactga ttgattactt taaagaggtt aacacggttg aagcttc
//
LOCUS       DAOTCW010000097         8387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_5896_length_8387_cov_44.744959, whole genome shotgun sequence.
ACCESSION   DAOTCW010000097 DAOTCW010000000
VERSION     DAOTCW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5896_length_8387_cov_44.744959"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="P5335_08370"
     CDS             complement(<1..1268)
                     /locus_tag="P5335_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRZ74930.1"
                     /translation="MQLTAEIIQKLESMVGPSFIFTDNETRNNYGHDETEDFVFPPNV
                     VVKPSNTLEISEILKLANQFKIPVVPIGARTGLSGGALSVQGGIGLSMERFNKILHID
                     EQNLQVITEPGVITQVLREAVAEKGLFYPVDPSSMGSCFIGGNVAENSGGARAVKYGV
                     TKDYVLNLEVVLPTGDIIWTGANTLKNSTGYNLTQLMVGSEGTLGIVTKIVLKLLPKN
                     THNVLMLVPFYKAHEACEAVSAIFRAGIIPSALEFMERDAIDWTLQFVDGLNVTVNED
                     IQAHLLIEVDGNNPEILMAEAEKIMMVVEQFQIDDILFADTEDEKNALWKMRRSVAEA
                     VKANSIYKEEDTVVPRYELPKLLNGIKTIGKKYGFQSVCYGHAGDGNLHVNIIKGTMS
                     DENWKTEVPKGIREIFELTVSLKGTLSGEHG"
     gene            complement(1302..2213)
                     /locus_tag="P5335_08375"
     CDS             complement(1302..2213)
                     /locus_tag="P5335_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRZ74931.1"
                     /translation="MDTIFLVFFCLLLGFGLQFLKSFPKNGYVALNHFVIYISLPALA
                     LYYIPKIEISSKLLFPIGVAWLGFALSFIFFYSLGKWLGWSKKLIGCLVLTGGLSNTS
                     FIGFPIIEALYGPEGLQTAIVVDQPGSFVVISTLGIAVASIFSKGDLDSKLIVRKIVF
                     FPPFIAFVLACILNLAKIDFPLFLQSVFQKVGGTVTTIALVSVGLQLKIDQQSRHWSF
                     LALGLFFKLMLMPFFFIILYKVIFNGQGLAVDVSIMESAMAPMITGAILATTYGLKPK
                     LSSMMIGVGIPLSFITLAFWYWILSLL"
     gene            2320..3411
                     /gene="meaB"
                     /locus_tag="P5335_08380"
     CDS             2320..3411
                     /gene="meaB"
                     /locus_tag="P5335_08380"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRZ74932.1"
                     /translation="MTKPNSKSSALHEKAGVESPETINLRSVETVQKFRRKSTTSDEL
                     VAGILKGNITFLSRAITLVESTNADHLAKANEVIQKCLPHANKSIRIGITGVPGVGKS
                     TFIEAFGKQLTSLGKKVAVLAVDPSSTISHGSILGDKTRMEELVKDKNAYIRPSASGE
                     TLGGVARKTRETIILCEAAGFDTIIIETVGVGQSETAVHSMVDFFLLLKISGAGDELQ
                     GIKRGIMEMADAIVINKADGDNIQRAKLAKTEFNRALHLFPAKKSGWMPTVSTCSSYT
                     KEGIENVWKTISDYFELTKTNHFFEEKRQEQNQYWMMETINEQLKNHFYTHPEIINIL
                     DKKKKAVSSNEISPFTAAMELLNNYFKEN"
     gene            complement(3419..3646)
                     /locus_tag="P5335_08385"
     CDS             complement(3419..3646)
                     /locus_tag="P5335_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74933.1"
                     /translation="MDLPKFVLADNTDFPEDIFIIHLEYPRFIINLKDDEVEIIEEVD
                     VEDEKDLETEMEKLIELAGAFYDREMERYEE"
     gene            complement(3700..4878)
                     /locus_tag="P5335_08390"
     CDS             complement(3700..4878)
                     /locus_tag="P5335_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRZ74934.1"
                     /translation="MSSDTSKRYALRGVSAAKEDVHNAIKNIDKGLFPKAFCKIVPDY
                     LTGDNEYCLLMHADGAGTKSSLAYLYWKETGDISVWKGIAQDALIMNIDDLLCVGATD
                     NIMLSSTIGRNKNLIPAEVISAIINGTEELIQELKSFGVTIHSTGGETADVGDLVRTI
                     IVDSTVTARMKRSDVIDNANIQPDDVIVGLTSFGQASYEKEYNGGMGSNGLTSARHDV
                     FAKYLAEKYPESFDAAVPSDLVYSGQVKLTDEVKNSPIDAGKLVLSPTRTYAPIIKTI
                     LEKYSPKEIHGMVHCSGGAQTKILHFIDNLHIIKDNLFPIPPLFKLIQEQSKTDWKEM
                     YQVFNCGHRMELYVPAAIANDIIEISKSFNVDAQIVGRVEASEYKKLTITSEFGTFEY
                     "
     gene            complement(4953..5840)
                     /locus_tag="P5335_08395"
     CDS             complement(4953..5840)
                     /locus_tag="P5335_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRZ74935.1"
                     /translation="MKSIFSLFSLFFSILVLQAQEQVSYYFDSNKSELKKTEFQKLND
                     WIETNKDIKIVAINGFTDEDGTIGFNDTLAQKRVDFIYNSIKDKVKIREDFKTRSFGE
                     QHSQSEKKAENRKVTIYYIEAKDLAREDEILGIKKEETVILPKQKKQFPQSIIIQNPN
                     GTESEFSLNVEFMQKIDEAQPGEKLKIDNLNFQINTFIIVNESRGKLYELLIVMQQNP
                     ELKIDIQGHLCCMPTDRTNLSTQRAKAVSQFLRMNGIENSRVTYQGFGSSNPIYPLPE
                     KNEEERAANRRVEILIIQN"
     gene            complement(6204..>7094)
                     /locus_tag="P5335_08400"
     CDS             complement(6204..>7094)
                     /locus_tag="P5335_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HRZ74936.1"
                     /translation="PEEKTDLKLNVVGKIPDVMLDNTDRNRTSPFAFTGNKFEFRAVG
                     SSANCANAMTTLNSIVAKQLKEFKKEVDALIENKDLKKDEAIFNVLREYIKGTKNILF
                     EGDGYSDAWEKEAKKRGLSNHKTTPAALKAKISKKALDLFKDLNVMNHIEVEARYEIE
                     LEEYTKKIQIEGRVLGDIARNHVIPTAIRYQNTLIENVRGLKEIFGKDFEKIAKEQIV
                     LIKEISFHIEGINTNVEAMTETRKKANALTDAQKMAEMYCDKVKPYFEVIREHCDKLE
                     LLVDDEVWTLTKYRELLFTR"
     assembly_gap    7095..7194
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7195..8201)
                     /locus_tag="P5335_08405"
     CDS             complement(<7195..8201)
                     /locus_tag="P5335_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRZ74937.1"
                     /translation="MSTLRFQALKDASSRKPVKFEESGKKSALFGSNVFNDKAMKQYL
                     TSDAYKGVKDAVQHGTKIDRKLADYIAMGMKEWALAKGVTHYTHWFQPLTGSTAEKHD
                     AFFETSYDGSDPVEKFGGGQLVQQEPDASSFPNGGIRNTFEARGYTAWDPTSPAFIFG
                     TTLCIPTVFISYTGEALDYKTPLLRALHSVDEAATDVCKYFDKNVKKVTATLGWEQEY
                     FLVDSALANSRPDLMLTGRTLLGHTSAKGQQLDDHYFGSIPSRALNYMRELEEECMLL
                     GIPVKTRHNEVAPNQFELAPIFEETNLAVDHNSLLMDVMSKVGERHDFKVLFHEKPFK
                     GV"
BASE COUNT         2695 a         1551 c         1299 g         2742 t
ORIGIN      
        1 ccgtgttcac ctgaaagtgt cccttttaat gaaaccgtta attcaaaaat ttcccgaatt
       61 cctttaggca cttcagtttt ccaattttca tcggacatgg ttcctttgat gatattgaca
      121 tgcaaattac catcaccggc atgaccataa caaaccgatt gaaaaccata tttttttccg
      181 atagttttga ttccgtttaa taatttaggc aattcatagc gaggcacaac cgtgtcttct
      241 tctttataaa tcgaattggc tttcactgct tcagccacac tgcgacgcat tttccacaag
      301 gcattttttt catcttcagt atccgcaaaa agaatatcat caatttgaaa ttgttctacc
      361 accatcatga ttttttcggc ttcagccatc aaaatttcag gattatttcc atccacttca
      421 atcaataaat gagcttgaat atcctcatta actgtaacat tcaatccatc cacaaattga
      481 agtgtccaat caatggcatc acgttccata aattccaacg cacttggaat aattccggct
      541 ctaaaaatag ctgaaacagc ttcacaggct tcatgagctt tgtaaaacgg aaccaacatc
      601 aatacgttgt gtgtattttt gggaagcaat ttcaaaacaa ttttggtgac gattcccaat
      661 gttccctcac taccaaccat caattgcgta agattatatc ctgtggaatt ttttaacgta
      721 ttggctccgg tccaaatgat atcaccggtt ggtaaaacta cttctaaatt taaaacataa
      781 tctttggtca ccccatattt caccgctctg gcaccacctg aattttctgc cacatttcca
      841 ccgataaaac agctccccat gctacttggg tcaacgggat aaaacaatcc tttttcagca
      901 actgcttcac gtaacacttg tgtaattact ccgggttcgg tgattacttg caaattttgt
      961 tcgtcaatat gtaaaatctt gttgaatctt tccatcgaga gcccaattcc tccttgaacg
     1021 gataaagctc ctccactcaa ccccgttcta gctccaatgg gaacaactgg aattttaaat
     1081 tgattagcca atttcaaaat ttcagaaatt tcaagagtat tggaaggctt cacaactaca
     1141 tttggaggaa aaacaaaatc ttccgtttca tcatgaccat aattatttcg ggtttcatta
     1201 tctgtaaaga tgaatgaggg tccgaccatt gattctaatt tttgaattat ttctgccgta
     1261 agttgcatat gcgtattttt aattgaataa aggtaaaaaa attatagtaa ggaaagtatc
     1321 caataccaaa atgccaacgt aataaaagac aatggaattc caacgccaat catcatgctg
     1381 ctcaattttg gttttaatcc gtaggtcgtt gctaaaatag ctccggtaat catcggtgcc
     1441 atagcggatt ccatgataga aacatcaacg gctagtcctt gtccattgaa aataacttta
     1501 tacaaaatga taaaaaagaa aggcattagc attaatttaa aaaataatcc cagagccaga
     1561 aaactccaat ggcgactctg ttgatcaatt ttgagttgta acccaacgga aactaaagca
     1621 attgttgtta cagtacctcc cactttttga aaaacagatt gcagaaataa aggaaaatca
     1681 atttttgcta agttcaagat acatgccaat acaaaggcaa taaatggcgg aaaaaacact
     1741 atttttctaa ctattagttt agaatctaaa tcaccttttg aaaaaattga agcaactgca
     1801 atccctaatg tggaaataac aacgaaagaa cccggttgat cgacaacaat agccgtttga
     1861 agtccttccg gtccgtataa tgcttcaatt attggaaatc caataaaaga tgtattactt
     1921 aaaccaccgg tcagaacaag acatccaatc aactttttgg accaacccaa ccattttcct
     1981 aaactgtaaa agaaaataaa ggacaatgca aatccaagcc aggccacacc aatcgggaaa
     2041 agtaacttgg aacttatttc tattttagga atataataca aggctaatgc cggcagagaa
     2101 atataaataa caaaatgatt taaagctaca taaccatttt tgggaaagga ttttaaaaac
     2161 tgtaatccga aacccaataa aagacaaaaa aagactagaa aaatagtatc catacttgac
     2221 attctgacct caaaaataca cttttaagtg gataaaatcg gcacgaaaaa tgaagcatcc
     2281 tttttctttt tgtaacttta cctttcaaat aatagcattg tgactaaacc aaattcaaaa
     2341 tcatccgcct tacacgaaaa agcaggggtt gaatcacctg aaacgataaa cttacggtca
     2401 gtagaaaccg tccaaaaatt tcgaagaaaa tcaaccactt ccgatgaatt ggttgccgga
     2461 attttaaaag gaaatattac tttcttaagt cgggctatta ctttagttga aagcaccaat
     2521 gctgaccatt tagcgaaagc caatgaagtc attcagaaat gtttgcctca tgccaataaa
     2581 tctattcgaa tcggaatcac cggtgttccc ggtgttggaa aaagcacctt tattgaagca
     2641 tttggaaaac agttaacttc cttaggaaaa aaagtagctg ttttagcggt tgatcccagc
     2701 agtacgattt cacacggcag tatattggga gataaaacca gaatggaaga attagttaaa
     2761 gataaaaacg cttacatccg gccttccgct tcaggagaaa ctttaggtgg tgtagccaga
     2821 aaaacacgtg aaaccattat tctttgcgaa gcagcagggt ttgataccat tattattgaa
     2881 actgtagggg ttggccaaag tgaaacagcc gttcacagta tggtggattt ctttttattg
     2941 ttaaaaattt ccggtgccgg agatgaattg caaggaatca aacgcggaat catggaaatg
     3001 gctgatgcta tcgttatcaa caaagccgat ggagacaaca tacaacgagc taaattagcc
     3061 aaaacagaat ttaatcgggc attgcattta tttcctgcaa aaaaatcggg ttggatgcca
     3121 accgtttcta cttgtagttc ctataccaaa gaaggaattg aaaacgtttg gaaaaccatt
     3181 tcagattatt ttgaattgac caaaaccaat catttttttg aagaaaaaag acaagagcag
     3241 aatcaatatt ggatgatgga aactatcaac gaacaattga aaaatcattt ttatacccat
     3301 cctgaaatta ttaatatatt agataaaaag aaaaaagcag tttcttctaa tgaaatttca
     3361 ccttttacgg cggcgatgga attgctaaat aattatttta aagagaatta gcaaaaaact
     3421 actcctcata tcgttccatc tcccgatcat aaaaagctcc ggctaattca ataagctttt
     3481 ccatttcggt ttccaagtct ttttcatctt ctacatcaac ttcttcgata atttcaactt
     3541 catcatcttt taaattgatt ataaaacgag gatattctaa atgaatgata aaaatatctt
     3601 cgggaaagtc agtattgtca gctaaaacga attttggtaa gtccatattt taaaggttag
     3661 cgggttgaaa gttacgagtt acaggttaaa aatttgagtt taatattcaa atgtaccaaa
     3721 ttcactggta atcgttaatt ttttatattc agatgcttct actcttccga caatttgtgc
     3781 gtcaacattg aatgattttg aaatttcaat aatgtcgtta gcaattgctg ccggaacata
     3841 caattccatt cggtgaccgc aattgaagac ttgatacatt tctttccaat cggtttttga
     3901 ttgttcctga attagtttaa ataatggcgg aatcggaaat aaattatctt taataatgtg
     3961 caaattatca ataaaatgta aaatcttcgt ttgagcacct ccactacaat gcaccatgcc
     4021 gtgaatttct tttggagagt atttttctaa aatagtttta ataatcggag cataagttct
     4081 ggttggcgaa agcactaatt tcccagcatc aatcggactg tttttcacct catctgtcaa
     4141 ttttacctga ccggaataaa ccaaatcgga aggaacagca gcatcaaaac tttcgggata
     4201 tttttcagct aaatacttag caaaaacatc atgacgagca gacgttaaac cgttacttcc
     4261 cattccaccg ttatattctt tttcgtagga ggcttgtccg aatgaagtca atcccacaat
     4321 tacatcgtca ggttgaatgt tggcattatc aatcacatcg cttcgtttca tacgagctgt
     4381 cacggtggaa tctacaatga tggttcgaac caaatcaccc acatcagctg tttctcctcc
     4441 ggtggaatga atggtgacac caaacgattt tagttcttga attaattctt ccgttccgtt
     4501 aattatggca gaaattactt ccgcaggaat tagattttta ttccgtccaa tggtggagga
     4561 aagcataata ttatcggtcg cacccacaca aagtaaatca tcaatgttca tgataagagc
     4621 atcctgagca attcctttcc acacggaaat atctccggtt tctttccaat ataaataagc
     4681 taaagacgat tttgttccgg ctccatcggc atgcattaac aagcaatatt cattatcacc
     4741 ggttaaataa tcgggtacaa ttttacagaa agctttggga aataaacctt tatcaatatt
     4801 ttttatggca ttatgcacat cttctttggc ggcagaaact ccgcgaagag cataacgttt
     4861 gctggtatcc gaactcatag ttgtgtagtt gtgtttggca aagataggat taatgtgaaa
     4921 atgtcacaat ttaaaaacac aataggtaaa agttaatttt gtattatcaa tatttcaact
     4981 ctacgattag ctgcacgctc ttcctcattt ttttcaggta acggatagat gggatttgaa
     5041 cttccaaaac cttgataagt cacccgtgaa ttttcaattc cgttcattcg caaaaattga
     5101 ctaacggctt tagcacgctg ggtagacaaa ttcgttcggt cagtaggcat gcaacataaa
     5161 tggccttgaa tatcgatttt aagttcggga ttttgttgca tcacgatgag taattcatat
     5221 aattttcctc gactttcgtt tactatgata aaagtattta tttgaaagtt tagattgtct
     5281 atttttaatt tttcacccgg ctgagcttca tctatttttt gcataaattc cacattcagt
     5341 gaaaattctg actcagttcc gttagggttt tggataatta ttgattgtgg gaattgtttt
     5401 ttttgcttag gaagtataac tgtttcttct ttttttatcc ccaatatttc atcttctcta
     5461 gctaaatctt ttgcttcaat gtaataaatt gtaacttttc tattttctgc ctttttttca
     5521 ctttgcgaat gttgttcacc aaaacttcga gttttaaaat cttctctaat tttaacttta
     5581 tcttttatac tattataaat gaagtcaact cttttttgag ccaaagtatc gttaaaacca
     5641 atcgttccat cttcatctgt gaaaccgttg attgcaacaa ttttgatgtc tttatttgtt
     5701 tcaatccaat cgttcagttt ttgaaattct gtttttttaa gttccgattt attagaatca
     5761 aaataatatg aaacctgttc ttgagcttga agaaccagaa tagaaaaaaa aagtgaaaac
     5821 aaactgaaaa tagatttcat aagggaattt tataatacaa cgaaaataca aaataataat
     5881 ttcataatgt ctataaaaaa aagtgggata actttcgtca tcccacccgt tttagtgcta
     5941 atttgttgga aatttagttt tgttgtgatc aaaatcaaaa agattttgat gtagtatttt
     6001 tgaacacaga aaactcactg ttaaatacca ctatacttcc acggctatat ctctagattt
     6061 ttttcaatgc gggggcattt gttgcttaaa gcgatacgaa aatataaaaa tattttatac
     6121 tgcaaccatt ttcattaaaa aatttaatta aaagtaaaaa agccacgttt gaaacgtggc
     6181 ttttttaaag cataatctat tgattatctt gtgaataaca attctctgta ctttgttaat
     6241 gtccaaactt catcgtcaac taaaagttcc aatttatcac aatgttcacg aataacttca
     6301 aaatatggtt ttactttatc acaatacatt tcggccattt tttgagcatc tgttaaggca
     6361 tttgcttttt tacgagtttc tgtcatcgct tctacattag tatttattcc ttcaatatga
     6421 aaagaaattt ctttaattaa aacgatttgt tctttggcta ttttttcaaa atcttttcca
     6481 aaaatctctt ttaatcctct gacattttca attaatgtat tttgataacg aatagcggtt
     6541 ggaatcacat gattacgagc aatatctcca agaactctac cctcaatttg gattttctta
     6601 gtatattctt ccaattcgat ttcgtaacga gcttccactt cgatgtggtt cattacattt
     6661 aaatctttga ataaatccaa tgctttttta gaaatttttg ctttcaaagc agccggagta
     6721 gttttgtgat tacttaaccc gcgtttttta gcctcttttt cccaagcatc actataacca
     6781 tctccttcaa acagaatgtt tttagttcct ttgatatatt ctcttaatac attaaaaatt
     6841 gcttcgtctt tctttaaatc tttgttctcg attaatgcat caacttcttt tttgaattct
     6901 tttaattgtt tcgccacaat agaatttaaa gtagtcatcg catttgcaca gtttgcagaa
     6961 gaaccaactg ctctaaactc aaatttattt cctgtaaaag caaaaggaga agttctgttt
     7021 ctatccgtat tgtctaacat aacatccgga attttaccaa ctacattcaa tttcaaatcg
     7081 gttttttcct caggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacacct
     7201 ttaaatggtt tttcatggaa cagtacttta aagtcatgtc tttcaccaac tttagacatt
     7261 acgtccatca aaagtgaatt gtggtcaacc gctaaattgg tttcttcaaa gattggagct
     7321 aactcaaatt gattaggagc aacttcatta tgacgggttt ttaccggaat tcctaaaagc
     7381 atacattctt cttccaattc tctcatataa tttaatgcac gagaaggaat tgaaccgaaa
     7441 taatgatcat ctaattgttg tcctttggca gaagtgtggc ctaacaacgt tcttcctgtc
     7501 aacattaaat caggacgaga gtttgctaaa gctgaatcaa ccaaaaaata ttcttgttcc
     7561 caacctaaag ttgcggttac ttttttaacg tttttatcaa aatatttaca tacatcggtt
     7621 gctgcttcgt caacggaatg aagagctctt aataaaggtg ttttataatc taatgcttcg
     7681 ccagtataag aaatgaatac agttggtata cataaagttg ttccgaagat aaatgccgga
     7741 gaagttggat cccaagcggt atagccacga gcttcaaaag tatttctgat tcctccattt
     7801 ggaaaagaag aagcatcagg ctcttgttga actaattgac caccaccaaa tttttcaacc
     7861 gggtctgaac catcatatga tgtttcaaaa aaagcatcat gtttttctgc cgttgaaccg
     7921 gttaaaggtt ggaaccaatg cgtataatgt gttacgcctt ttgctaaagc ccactctttc
     7981 atacccattg cgatataatc tgctaatttt ctatcaattt ttgttccatg ctgaacggca
     8041 tctttaactc ctttataagc atctgaagtt aaatactgct tcatcgcttt atcattaaac
     8101 acattggatc caaaaagagc cgattttttt ccggactctt caaattttac aggttttctg
     8161 cttgatgcat cttttaatgc ttggaaacgt aaagttgaca taaaattatt tttaaaggtt
     8221 aaaccacatt attttatggt gcaaatatat ttaaaaatta tttcaaaaca agtatacccc
     8281 tattttttta ggggtaaaat ctataaatcg taaatattat cgattaaatg tggctaaatt
     8341 ttatgaattt gtcagtttta cggattttga gtcacaaaat tttttag
//
LOCUS       DAOTCW010000098         8316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_5977_length_8316_cov_38.769156, whole genome shotgun sequence.
ACCESSION   DAOTCW010000098 DAOTCW010000000
VERSION     DAOTCW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5977_length_8316_cov_38.769156"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="P5335_08410"
     CDS             complement(<1..959)
                     /locus_tag="P5335_08410"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRZ74938.1"
                     /translation="MLFNSLSFIVFLVVVVALYYSNLLSWNNRKRMLLFTSYVFYGLW
                     NPPLVIILWLSTIVDWVAANNLAVEENQRKRNGWLALSMVVNLGFLGFFKYGNFLLEN
                     FTYLMNTIGVEYQAQPMDIILPMGISFYTFQTMSYTLDVYNRKAKPANTFLDFALYVT
                     FFPQLVAGPIVRAGELIEQFYQEKKATVEQFIWGLFLFTLGLFQKVVLADSLLSDIAD
                     TVFGSKEALNFWDAWAGTLAFSGQIFFDFAGYSTCAIGIAMMLGFVLPHNFLYPYGAI
                     GFTDFWRRWHITLSTWLRDYLYIPLGGNRLGMTRMYVALMVTML"
     gene            complement(966..2006)
                     /locus_tag="P5335_08415"
     CDS             complement(966..2006)
                     /locus_tag="P5335_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74939.1"
                     /translation="MNLKKSLLITIILSLAAIGVWEVYWRSKGYFPDLDDDKFLWART
                     RAKVDKASSNDVVLIGSSRVLFDIQVPQWEKQTGIKPIQLANAGATPLPIFHDIVENS
                     TFNGTIVVGVTPGLFFSTTFPQAPPWDRAASRAKFYEDQTYAQQLNYALSIPLQKTFA
                     FISNDEEEWYDDINLKALLKTIQLPKRTAKPDMPPFYRFQDIDIDRNVRMKQKVVTDT
                     AFANSVKTVWKFFMSVHRPPPDKEGTMAFFLKDLEKFKARGGKVILVRLPSSGFFHDL
                     ETNGLPRKDFWDVLVKKANVPAYYYSDYEKLSGFDTPEWSHLSGPDATLFTERFVNIL
                     LEDNLITNTKPN"
     gene            complement(2033..>3271)
                     /locus_tag="P5335_08420"
     CDS             complement(2033..>3271)
                     /locus_tag="P5335_08420"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HRZ74940.1"
                     /translation="PTQNNERETFMDALRGFAILGIFIANLAHLSFYNEGAKIVSPYI
                     VEGWDHSVRFLHYLFIEGKFYSIFSLLFGWGIALQLKRGATKGIDSLPIVKRRLLFML
                     LLGALHLLIWTGDIVFFYALLGFIVLPLRKFSNKTLLITGVLLILSPILLYGLKMTFP
                     ILNYPAEKLYETGDRITTFFFGEMTREKYMNLMHEGSWYDQVKVNLAEFFYRYGYLFY
                     VSRISKVVGMFLIGFVIGRSDFYKNLQQHKKTVYKIIGIGLVVGLVFNYFLASYMSSS
                     ISGEYYNLKESGWYLTIFYALGVVPMALTYVGLMMLGFQTKLGNRLLQVVAPVGKMAF
                     TNYLMHSLIGNFVFLGAGLDMMGQVGPVFFTLFGITVFVFQIIFSTLWLNHFQYGPAE
                     WVWRSLTYKKRQPFLVISQN"
     assembly_gap    3272..3371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3372..4270)
                     /locus_tag="P5335_08425"
     CDS             complement(<3372..4270)
                     /locus_tag="P5335_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HRZ74941.1"
                     /translation="MKTNFILLFIVTTLLLSCSEKQNSTTENKSTSSYFDYSNSDDQL
                     TGGIKMIPIKTAKGTFNVFTKRVGNNPKMRVLLLHGGPGGTHEAFAAFDGYFPNEEIE
                     YIYYDQLDSYYSDKPNDSTLWTTERFVEEVEQVRIALNLTKDNFYLLGHSWGGILAME
                     YALKYQDNLKGLIISNMMMSAPAYGKYANEVLGPQLKPEILKEIKDLEAKNDFSNPRY
                     SELLMNYYYTEHILRMPLEKWPEPINRMFKHLNPNIYVYMQGHSEFGMTGNATLKNWD
                     VTSQLKTIKTPTLVIAGTYDTMDP"
     assembly_gap    4472..4571
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4572..5443)
                     /locus_tag="P5335_08430"
     CDS             complement(<4572..5443)
                     /locus_tag="P5335_08430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRZ74942.1"
                     /translation="MNQLTSFINKSKLFLTENTVLRYLNFSALYVAQGIPEGITYFAI
                     PAWLAMNGKTPMEIGSFVAVIGIPWSFKILIAPIMDRYTILSMGRKRPWIILGQLGLI
                     LSFLSFIFIKDPVNNLPGLMICGFFISFFGAFQDVATDGMAIDVVPVNEQARANGIMW
                     GSKTVGTALSLMVGTTLINTVGFSTAIASLSIAVALIILVPIFFRERPGEKLMPWTKG
                     EASSDSIKNQLDDWSHIFKNLIKVVPLSSSIVMGISVLIIGLMFGLMDTILPIFTIQE
                     LGWTNTTYSEIYAS"
     gene            complement(5479..6348)
                     /locus_tag="P5335_08435"
     CDS             complement(5479..6348)
                     /locus_tag="P5335_08435"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRZ74943.1"
                     /translation="MKKILITGCSSGFGFQAAKYLAEKGHHVYATMRNTNSSNKEAAE
                     NLKKCAAEKNLKIEVVELDVTSDESVKTAMTKIPTVDVLINNAGRGFGGPIEAFSAAE
                     CLAQLDLNIVGNIRMAKAVLPGMRAQKSGLIIQLSSIAGRLAVPGFGIYHASKWGVEG
                     LSEAMRYELAPLGIDVAIVEPGPFATNFFPSLIIAKDEDIAKAYEHVAAFSAEFGSKT
                     QAAFEDENAPTDPMIVVQLFEELINASAGSRPLRNVAGLDFGARSIIDVIEPLRKGVL
                     DAFQLSDWDGVKA"
     gene            complement(6372..7250)
                     /locus_tag="P5335_08440"
     CDS             complement(6372..7250)
                     /locus_tag="P5335_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRZ74944.1"
                     /translation="MKSIFQKVQLLLVLGFTTTLLAQTQVEKNIKMYSTTWDNIVNKR
                     QIDLINAANFDANITMLSNPENIVGIENFKAHYQNYLTGFSDITFTIVEIFGQGDKIV
                     KHWNFKGKHTGTFFGIPPTGKSVNIQGVTIAKMKNGKIAQEQDFMDSMVFLQQLGIVS
                     NPNNLTIVDNLYKSFAKGDVPSVLAAMNSDIIWNEAEGNKYADGNPYKGPEAVLQGVF
                     GRILAEHEYFNLDEIQLHEMSNNQVLATLRYKAKLKNGNEYNAQVAHFWTLKDGKVIA
                     FQQYVDTKKLNDALIK"
     gene            complement(7339..7863)
                     /locus_tag="P5335_08445"
     CDS             complement(7339..7863)
                     /locus_tag="P5335_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRZ74945.1"
                     /translation="MKKIIFILVTLATVVACQNKVRYTQKSAEIETYKKSIKDYEMAN
                     WESLATHYADTAKIMNNVTDKNGISVAQNIELNKKDISVLSSYGFVADESEYEMVETD
                     KGKTWVNFWGLWQGRLKANDQLIEIPVHLTAQFINGKIVSEHGYWDNTALVMALMKLE
                     EKMPTEQPLATTKE"
     gene            complement(8026..>8316)
                     /locus_tag="P5335_08450"
     CDS             complement(8026..>8316)
                     /locus_tag="P5335_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74946.1"
                     /translation="IFTDLIKERVSIIKEVEGSPRSFWYSYMGGGPNAPHYFVSTPFK
                     SFADMDVKRDNVWKLIEKAKGKKKADQLRDAGRAVIESSWSYIYVLNEELSK"
BASE COUNT         2560 a         1500 c         1396 g         2660 t
ORIGIN      
        1 agcatggtca ccattaacgc cacatacatt cgggtcattc ctaaacgatt tcctcctaac
       61 gggatgtata aataatcccg aagccaagtg gaaagtgtaa tgtgccagcg tcgccaaaaa
      121 tcagtaaaac caatcgcacc atatggatat aagaagttat ggggcaatac aaatcccaac
      181 atcatagcga ttccaatcgc acaggtggaa tatcctgcaa agtcaaaaaa tatttgtccg
      241 gagaaggcga gtgttcctgc ccatgcatcc caaaaattaa gagcttcttt actaccgaaa
      301 acagtatctg cgatatcaga caataaggaa tcagccaaaa ccactttttg aaagagtcct
      361 aatgtgaaca agaacaatcc ccaaatgaat tgttcgacag ttgccttttt ttcttgataa
      421 aactgttcga ttaattctcc ggctctcaca atcggaccgg cgactaattg cggaaagaaa
      481 gtaacgtata gtgcaaaatc taaaaaggta ttcgccggtt ttgcttttcg gttatagaca
      541 tctaaggtat acgacatggt ttgaaaggta taaaacgaaa ttcccatcgg aagaatgatg
      601 tccatgggtt gagcttggta ttcaacgcca attgtattca ttaaataagt gaagttttcc
      661 aataaaaaat taccatattt aaagaaaccg aggaaaccta aatttaccac catactcaag
      721 gctaaccagc catttcgttt gcgttggttt tcttctacag ccagattgtt tgcggctacc
      781 caatctacaa ttgttgataa ccatagaata attaccaatg gtggattcca taatccataa
      841 aatacataac tggtaaacaa tagcattcgt tttctattgt tccagcttaa aagattggag
      901 taatacagag ccactactac aaccaaaaaa actataaaac ttaaggaatt gaaaagcata
      961 gttatttagt tgggtttagt gttagtaatt aggttgtctt ctaataaaat attcacaaat
     1021 cgctcggtaa aaagagttgc atccggccct gacaaatgcg accattccgg tgtgtcaaag
     1081 ccactcagtt tttcgtaatc tgaataataa taggcgggaa cgttggcttt ctttactaaa
     1141 acatcccaaa agtcttttcg gggtaaacca ttcgtttcta aatcatgaaa aaaaccggaa
     1201 gaaggtaatc gcaccaatat cacttttccc cctcgagctt tgaatttttc aagatctttg
     1261 aggaagaaag ccattgttcc ctctttatcc ggaggcggtc tatgaactga cataaagaat
     1321 ttccaaacgg tttttaccga attagcaaaa gcagtatctg taactacttt ttgtttcatg
     1381 cgaacatttc gatcgatgtc aatatcctga aatcgataaa aaggaggcat atcaggtttg
     1441 gctgttcgtt taggtaattg tattgtttta agcaatgctt ttaaattgat gtcatcatac
     1501 cattcttctt catcgttgga tatgaatgca aacgtttttt gcaagggaat agatagggca
     1561 tagtttaatt gttgagcata ggtctggtct tcataaaatt tagctctcga agcggctcta
     1621 tcccaaggcg gagcttgtgg aaatgtggtt gaaaaaaata atccgggggt aacacccact
     1681 actattgtac cgttaaaagt tgaattttcc acgatgtcat gaaaaatggg aagaggagta
     1741 gcaccggcat ttgctaattg aatgggtttt ataccggttt gtttttccca ctgaggcact
     1801 tgaatatcaa acagtaccct agatgaacca atgagtacca catcatttga agaagctttg
     1861 tcaacttttg ctcgtgttct tgcccaaaga aatttatcgt cgtctaaatc cggaaagtat
     1921 cctttggaac gccaataaac ttcccatact ccaattgctg cgagactcag tataatggta
     1981 attagaagtg atttttttag gttcatacag atgtttttgg ttaggttttc aattaattct
     2041 gggaaatcac taagaaaggt tgtcgttttt tataggtaag tgaacgccaa acccattcgg
     2101 cggggccata ttgaaaatgg ttgagccaaa gcgtgctgaa aataatttga aaaacaaaaa
     2161 cggtgatgcc aaataatgtg aaaaagaccg gacctacttg tcccatcatg tctaaaccgg
     2221 ctcccagaaa cacaaaattt ccaatgagtg aatgcatcaa ataattggta aaagccattt
     2281 ttccaacggg agctaccact tgtaaaagtc tatttcccag tttggtttga aaacctaaca
     2341 tcatcaatcc aacatacgtt aaagccatgg gaacaactcc taaagcataa aaaatagtga
     2401 ggtaccaacc gctttctttt aagttatagt actctccact tattgacgat gacatgtaag
     2461 aagcaagaaa ataattaaaa acaagcccaa cgactaaacc aatccctata attttataaa
     2521 cggttttctt gtgttgttgc aggtttttat aaaaatcaga acgtccaatt acaaaaccaa
     2581 ttagaaacat tcccactact tttgaaattc ggctaacata aaacaaataa ccgtagcgat
     2641 agaaaaattc tgctagattg actttaactt ggtcatacca agagccttcg tgcatcaaat
     2701 tcatatattt ttctcgggtc atttcaccaa agaaaaaagt agtgatgcga tcaccggttt
     2761 catagagctt ttcagccgga taattgagta tggggaaagt catttttaat ccatatagta
     2821 aaataggaga gaggataagc agcaccccgg tgattaataa agttttattg gaaaacttac
     2881 gtaagggtaa aacaataaaa cccaataaag cataaaagaa aacaatatca ccggtccaaa
     2941 tcagtaaatg taacgctcct aataataaca taaaaaggag tcttcttttg acaattggta
     3001 gactgtcaat tccttttgtt gcaccacgtt ttaattgaag tgcaattccc catccaaaca
     3061 ataagctgaa aatagagtag aattttcctt cgataaacag gtaatgcaaa aaccgaacgg
     3121 aatgatccca accttcaaca atgtatggac ttacaatttt agctccttca ttatagaaac
     3181 ttaaatgagc caaattggca ataaaaatac cgagaatagc aaaaccccgt agggcgtcca
     3241 taaaggtttc ccgttcatta ttttgggttg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn ngggtccatc gtatcatacg ttcctgcgat gactaaagtt ggtgttttaa
     3421 tagttttaag ttgactagtt acatcccaat tttttaaagt ggcatttccg gtcatgccaa
     3481 attcactatg tccttgcatg taaacataaa tgttaggatt taaatgtttg aacattctgt
     3541 taattggttc cggccatttt tcaagaggca ttcgtaaaat atgttcggta taataataat
     3601 tcatgagtaa ttctgagtat ctcggattac taaaatcgtt cttggcttct aagtctttaa
     3661 tttcttttag aatttcaggt ttcagttgtg gacctaacac ttcattggca tattttccat
     3721 aagcaggagc actcatcatc atgttagaaa tgattaaacc ttttagatta tcttggtatt
     3781 ttaatgcata ttccatggct aaaattcctc cccaggaatg tccgagtaaa taaaaattat
     3841 cttttgtgag gtttaaagcg attcgtactt gttctacttc ttctacaaaa cgttcagtag
     3901 tccaaagagt ggaatcgttt ggtttatcac tgtagtaaga atctaattgg tcgtagtaga
     3961 tgtattcaat ttcttcatta ggaaaatagc cgtcaaaggc tgcaaaagct tcgtgcgttc
     4021 caccgggacc gccatgtaat aatagcacac gcatttttgg attatttccc acacgtttag
     4081 taaatacgtt aaaagttcct ttagcggttt taatgggaat cattttgatg ccgccggtta
     4141 attggtcgtc actattggaa taatcaaaat aggaggaagt gcttttgttt tccgttgttg
     4201 aattttgttt ttctgaacag gatagtagaa gtgtggttac aataaaaagt aaaataaagt
     4261 tggttttcat actaattagt tttaggtttt tctttaatta ttttgaagag attacagtaa
     4321 aaatagttga tttttcctct tccgtgatga atccgtctat actttttttg tgtttgataa
     4381 aattgatgaa ttgaataaga attagcattg ttaaaggaat aagggcaata caaagataca
     4441 tttgactcca ttccattttt tctttcattg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn ncttgaagca taaatttctg aataggttgt gttagtccaa cccaattcct
     4621 gaatagtaaa tatgggtaaa atggtatcca ttaaaccaaa cattaaacca attatcaaaa
     4681 cagaaatgcc catgacaata cttgaagaaa gcggaacaac ctttataaga ttcttaaaaa
     4741 tatgactcca atcatccaat tggtttttta tagaatccga agacgcttct ccttttgtcc
     4801 acggcattaa tttttcaccg ggtctttccc taaagaatat gggtactaat ataatcagtg
     4861 ccactgcaat ggataaagaa gcaattgctg ttgaaaaacc aacggtattg attaaagttg
     4921 ttccaaccat taaagataaa gctgttccta ctgttttcga tccccacatg ataccgttgg
     4981 ctcttgcttg ttcattaaca ggaaccacat caatcgccat tccatcggtg gctacatctt
     5041 ggaaggctcc aaaaaagctg ataaaaaatc cgcaaatcat taatcccggt aaattattaa
     5101 ccgggtcttt aatgaaaata aaacttaaaa aactcaaaat taaacccaat tgccctaaaa
     5161 taatccaagg tcttttgcga cccattgata aaattgtgta gcggtccatt atcggagcta
     5221 ttaaaatttt aaaactccag ggaattccaa tgacggcaac aaaactgcca atttccattg
     5281 gcgtttttcc gttcattgcc aaccaagccg gtattgcaaa ataagtaatt ccttcgggta
     5341 ttccttgagc tacatacaaa gcagaaaaat tcaaatacct taaaacagta ttttcggtta
     5401 aaaacagttt gcttttgtta ataaaggaag taagctgatt catagacaat taatagattt
     5461 tgaaatagtt gaattgtgtt atgcttttac gccatcccaa tcggataatt gaaatgcatc
     5521 taatacacct tttcttaaag gttctatcac atcaatgatg cttctagcac caaaatccaa
     5581 tccggcaaca tttcgcaacg gacgagaacc tgcggaagcg ttgattagct cttcaaataa
     5641 ttgcacgaca atcattgggt cggttggtgc attttcatct tcaaatgcgg cttgggtttt
     5701 gctaccaaat tctgcactaa aagcagctac atgttcgtag gctttggcta tatcttcatc
     5761 tttagcaata attaaactcg gaaaaaagtt tgtggcaaaa ggtccaggtt ctacaatagc
     5821 cacatcaatt ccaagtggag cgagttcata acgcatggct tcacttaatc cttccacacc
     5881 ccatttactg gcgtgataaa ttccaaaacc gggaacggct aacctaccgg caatagaact
     5941 aagttgaata atcaaacctg atttttgagc ccgcattccg ggaagtacag cttttgccat
     6001 tcgaatattg cccacaatgt ttaaatctaa ctgtgctaaa cattcagcag ctgaaaaagc
     6061 ttcaatgggt ccaccaaatc cgcgacccgc attgttgatt aatacatcca ctgttggtat
     6121 ttttgtcatg gctgttttca cactttcatc tgaggtaaca tctaattcaa cgacctcaat
     6181 ttttaagttt ttttctgctg cacatttttt taaattttct gcagcctctt tatttgagct
     6241 atttgtgttt cgcatcgtcg cataaacatg atgtcctttt tctgcaagat attttgcggc
     6301 ttgaaatccg aaaccgctgc tgcaacctgt gatgagtatt tttttcattg ttctgttttt
     6361 ttgttgaact attattttat aagtgcatca ttcaattttt tagtatctac atattgttga
     6421 aaagcaatta cttttccgtc tttaagtgtc caaaaatgag ctacttgagc attgtattcg
     6481 ttaccgtttt taagttttgc tttgtaacgt aaagttgcta atacttggtt gtttgacatt
     6541 tcgtgtaatt gaatttcatc taaattgaaa tactcatgtt ctgctagaat cctaccaaat
     6601 actccttgta aaactgcttc cggaccttta tatgggttgc catcagcata tttgtttcct
     6661 tctgcttcat tccaaataat gtctgaattc atagcagcta aaacacttgg cacatcgcct
     6721 tttgcaaatg atttgtacaa attatctaca atcgttaaat tgtttggatt ggaaacaatc
     6781 cctaattgtt gaagaaaaac catactgtcc ataaaatcct gttcttgggc aatttttccg
     6841 tttttcattt tagcaattgt aactccttgg atattaacag atttgccggt aggaggaatg
     6901 ccgaaaaaag tgccggtatg tttacctttg aaattccaat gttttacaat cttatcacct
     6961 tgtccgaata tttcaactat tgtgaaggta atgtctgaaa atccggttaa gtaattttgg
     7021 taatgagctt tgaaattttc aattcctaca atgttttcag gattggaaag catggtaatg
     7081 tttgcatcaa aattagcagc attgatgagg tcaatttgtc ttttattgac aatgttgtcc
     7141 cacgttgtgg aatacatttt aatgtttttt tcaacttgtg tttgagccaa aagcgttgtt
     7201 gtaaatccaa gtacaagtaa aagttgtact ttttgaaaaa ttgatttcat gtttgttttt
     7261 tttgtttgat ataagaggtt taattcaata caaaactgtc caataaaaaa aataattgga
     7321 cagtattgag tttactattt attcttttgt cgtcgctaac ggttgttccg ttggcatttt
     7381 ttcctctagt ttcatcaagg ccattactaa agccgtgtta tcccaataac catgttcgct
     7441 tacaatcttt ccattaataa attgtgctgt taagtgaaca ggaatttcaa ttaattggtc
     7501 gtttgcttta agtcttcctt gccacagtcc ccaaaagttc acccaagttt tacctttatc
     7561 tgtctccacc atttcatatt ccgattcgtc cgcaacaaag ccgtaggaag acaagacaga
     7621 aatgtctttt ttatttaatt caatattttg agcaacagaa attccgtttt tatcggttac
     7681 attattcatt attttagccg tatcggcata atgtgtggct aatgattccc aattggccat
     7741 ttcgtaatct ttaatggatt ttttataagt ctctatttct gcagattttt gtgtgtaacg
     7801 tactttgttt tgacaagcta caacagtagc aagagtcact aaaatgaata tgattttttt
     7861 catgatttat atttttaaat ggttatccta aaacgctctt tttaagatta atgaagcaat
     7921 ttttatacac cttccaatca attattattt agcacggtta gtcataataa ttgattgagg
     7981 tgtaatgaat taataattga aaacatataa ccttctgata tgtatttatt tactcaactc
     8041 ttcgtttaaa acatagatat aagaccatga actttcaata actgctcttc cggcatcacg
     8101 caattggtct gctttctttt tgcctttggc tttttcaatc agtttccaaa cgttgtctct
     8161 ttttacatcc atatcagcaa aacttttaaa tggtgttgaa acaaaataat gaggagcgtt
     8221 aggaccgcca cccatgtagg aataccaaaa tgatcttgga cttccttcta cttctttgat
     8281 gattgaaact ctttctttga ttaagtcagt aaaaat
//
LOCUS       DAOTCW010000099         8306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_5988_length_8306_cov_50.049691, whole genome shotgun sequence.
ACCESSION   DAOTCW010000099 DAOTCW010000000
VERSION     DAOTCW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5988_length_8306_cov_50.049691"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="P5335_08455"
     CDS             complement(<1..218)
                     /locus_tag="P5335_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74947.1"
                     /translation="MKNYRQLTLSVILLTFSYSFFGQQLDRSSLPIKTPARQTSKELD
                     VRNVSVPERVEVKAPEGAPNVILVLVDDL"
     gene            complement(356..1330)
                     /locus_tag="P5335_08460"
     CDS             complement(356..1330)
                     /locus_tag="P5335_08460"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRZ74948.1"
                     /translation="MHSIQAYQEHFLNYLQQQHFLRDPKNLYEPIDYILGLGGKRMRP
                     VLTLMAAEVFDTNFEKALPAATAVEVFHNFSLVHDDIMDDAPLRRGKVTVHEKWNLNT
                     GILSGDAMLILAYQYFENYEPAIFKELAKLFSKTALEVCEGQQYDVDFETRDDVTISD
                     YLKMIEYKTAVLVGAAMKMGAIIAETSEENANYIYDFGLNLGIAFQLQDDYLDCFGDP
                     ETFGKQVGGDIIENKKTYLYLKAIEFAQAEERDQLLHLYSIQPEDNSDKIDSVKELFT
                     YSGASAATQKAIEDYTSKAFETLEQMQISGDKKMVLKAFGEKLMKRSV"
     gene            1554..2075
                     /locus_tag="P5335_08465"
     CDS             1554..2075
                     /locus_tag="P5335_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRZ74949.1"
                     /translation="MKTKWMMDPNQSDILIKMRHSIVAYMTGTQNKFDGYVEMKNNEI
                     EAASVEFLINVNNNETILEPKKAHLKLNNFLDKKEYPIISFKSTSFEKINNNINFLKG
                     NLTIKNITKVIELDAEYLGIKSYNGTEKAAFEITGDINRKDFGLAFTSFNQAGYLSSG
                     NNIKLIANLEFTV"
     gene            2088..2684
                     /locus_tag="P5335_08470"
     CDS             2088..2684
                     /locus_tag="P5335_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRZ74950.1"
                     /translation="MKEKIIAKATDLFLKLGFKSVTMDDIAGEMCISKKTIYKYFCNK
                     EILIAETTEIVHKAIHQSINTVVAKNHNAIEENFEIRKMFKEMFKSGEDSPSYQLKKH
                     YPEIYKKVMSREINECNFTFKQNIERGIKQGLYRKDLNPDTYVGFYYTLIFNIKESTS
                     SEKESRFLELQALEYHIRAMATKEGVLELEKQLHNYTL"
     gene            2684..4018
                     /locus_tag="P5335_08475"
     CDS             2684..4018
                     /locus_tag="P5335_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRZ74951.1"
                     /translation="MKTLFLIPLLVFGLTAEAQVETLTLKDALTYALENKAEARKSRL
                     EIENSEYQIQEIRSRALPQISANGGLTYNPVLQTNILDGAIFGQPGQSVQVAFGQKWT
                     SNAGVSLNQALFDQTVFTGLKAAKSTREFYQLNNQLTEEQVIERVANSYYSVYVQRER
                     LTLLDSNYINSTKVRDIVKGQYDNGLAKKIDLDRILVKISNIDTERQQVKNQIELQEN
                     ALKFYMGMPIENQFVIPQTEFEVTPISLAEVPNTANRSEYLLLKKQEELLEFQKTAIK
                     GEYYPTLSLVAGYNYLGQGPEMPLFAKPADGVYWSDYSAIGLNLRIPIFTGFGTRSRV
                     RQADIDIQILQEDIKDTQLALDLDYRNAKAQIENNLITIKNQSENMKLARDILDNTKN
                     NYLQGLATLTDLLDSQNESLDAQNNYTRAILNYKIAEIALIKSRGELKTLLN"
     gene            4025..5089
                     /locus_tag="P5335_08480"
     CDS             4025..5089
                     /locus_tag="P5335_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRZ74952.1"
                     /translation="MKKTLLIIITIVASLALVGYVLTKNKAANKAKTDIVAEKNAAVS
                     VKVAQVKTENVSLDFVANGNFAPTQELNFSAEKSGKVISVLVDEGDYVKVGQTLVIVR
                     SDVINVNAQTAKAAYENAKSDYARYENAFQTGGVTKQQLDQAKLQLTNAQSNLTQANI
                     NVGDTKVKAPIKGFVNKRYIEPGSILTGMPATQMFEIVDVSKLKLKVTVNESQVASLK
                     IGNTVDVTSSVFPDKVAKGKIIFIAPKADESLNFPVEIEITNSANSDIKAGMYGTATF
                     ATSQKQDLKVVPRNAFVGSVSSNQIFVVENGVAKLKKVTAGRILGDQVEIINGLSDGE
                     TVIVTGQINLQDGNAVEIIK"
     gene            5182..>8306
                     /locus_tag="P5335_08485"
     CDS             5182..>8306
                     /locus_tag="P5335_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRZ74953.1"
                     /translation="MKLAEISIKRPSLIIVLFTILTLGGLFSYSQLGYELIPKFETNV
                     ITVSTIYPGASPSEVENTITKKVEDGIASLENIKKIDSKSYESLSIVSITLTSNADID
                     ISMNDAQRKINAIVSDLPDDAETPALTKFSLSDLPIMTIGANGKMDEATFYDLIDKKI
                     SPVLSRVQGVAQVNIIGGQEREIQVNLDAVKMQGYGLSVPQVQQIILTSNLDFPTGNI
                     QTRDQKILIRLAGKYKNVEELRNLVISSNNGIEVRLKDIADVQDTQKIAEKISRVDQK
                     SAIILQIIKQSDANAVAVSEELKNAITKLETDYKNAELSLKIAKDSTDYTLEAADSVI
                     HDLLIAVILVAFVMLFFLHSIRNSLIVMVSIPASLIATFIGIYLLGYTLNLMSLLGLS
                     LVVGILVDDAIVVLENIYRHMEMGKNRVRAAFDGTAEIGGTVTSITLVIVVVFLPIAM
                     STGLVSNIITQFCVTVIISTLLSLLTSFTIIPWLSSRYGKLEHIEGKNLFGRIILGFE
                     SYLTRFTNWVSNLLNWCLDHYIKTIGVVLVLFFGAIALIPAGFIGGEFFASSDSGEFL
                     VQIEMPKDASLEQTNFMTQKAEAFLKNQEYVESQITTVGQTSEGMGASQATAYKAEID
                     VKMIDQAERTDDASVYAAKIKRKLEKVLVGAKVKTVPVGILGTAENAKLGLVVTGPNV
                     ESAMAFAKLALAELRTIPGTTEIKLTVEDGNPEINVQVDRDKMAALGLTLQTVGMTMQ
                     TAYSGNTDGKFRAGEYEYDINIKYNAFDRKNITDVSNLIFINNAGQQIKLSQFAAITE
                     GSGPSQLERRDKSASVTVQGQNVGVPTGTIVTQWQEKLDKLQKPTGVDYIWGGDQENQ
                     SEGFGTLGIALLAAIILVYLIMVGLYNSFAHPFVVLFAIPLSFIGALLALALTNKTLN
                     IFTILGIIMLIGLVCKNAIMLVDYTNQRRKAGETIRKALIQANHARLRPILMTTIAMV
                     FGMFPIALASGAGAEWKNGLAWVIIGGLISSLFLTLIIVPVIYEIMEKIITKFSKGEK
                     IDYETEMVADYV"
BASE COUNT         3015 a         1347 c         1430 g         2514 t
ORIGIN      
        1 aggtcgtcaa ccagcactaa tattacgttt ggagctcctt cgggagcttt aacctcaact
       61 cgttcaggta cggaaacatt tcgaacatca agctcttttg atgtttgcct cgcaggtgtt
      121 ttgattggta gtgaactacg gtcaagttgc tgaccaaaaa aggaatatga aaatgtcaat
      181 aaaatgacac tcaatgttaa ttgtctataa tttttcattg gaaaagcaga taaattagtg
      241 tttataaatt acgcactaaa tttacaaaaa aaaagattaa actaaatttt tataaaactt
      301 ttaaaagaaa aaataaatct ttacttttta ccaattataa ttttcagaca ttcttttaaa
      361 ctgatctctt cattaatttc tctccaaagg cttttaatac cattttttta tcgcctgaaa
      421 tttgcatctg ctctaacgtt tcaaatgctt tggatgtata atcttctatc gctttttgag
      481 ttgctgcaga tgctccggaa taagtgaaga gttccttaac cgaatctatt ttatctgaat
      541 tgtcttccgg ttgaatggaa tacaaatgca ataattgatc acgttcctcc gcttgtgcaa
      601 attctattgc tttgaggtat aaatacgttt ttttattttc aataatatca ccacctactt
      661 gtttaccaaa cgtttcagga tcaccaaaac aatctaaata atcatcttgt aactgaaaag
      721 caatacctaa atttaatccg aaatcataga tataatttgc gttttcttcg gaagtttcgg
      781 caataatagc acccattttc atggcagctc ccactaaaac ggccgtttta tattcaatca
      841 tctttaaata gtcagaaatc gtcacgtcat ctctggtttc aaaatcaacg tcgtattgtt
      901 gcccttcaca gacttccaat gctgttttgc tgaataattt agctaattct ttaaaaatag
      961 ccggttcgta attctcaaaa tactgatacg ctaaaatcaa catggcatca ccggataaaa
     1021 tgccggtatt caaattccac ttttcatgaa cggttacttt tcctcttcgc aacggagcat
     1081 cgtccataat atcatcgtga actaacgaaa aattatggaa aacctcaacc gcagtagccg
     1141 caggaagtgc tttttcaaaa tttgtatcaa acacttctgc tgccattaat gttaacacag
     1201 gtcgcattcg tttgccccct aaacctaaaa tgtaatcaat gggttcatac aaatttttag
     1261 ggtcgcgtaa aaaatgttgt tgctgaagat agtttaaaaa atgttcctga taagcttgga
     1321 ttgaatgcat gttcaattaa tagattgtgt tatagattca caaagaaaac caaaaaaagc
     1381 cgaatgcgaa aattgcagga aacattccat cttttaacaa atgttaaatt tatgtaaata
     1441 ctttggaaac tattttggtg ttaaaagttt cctgtttaca tttgtccctg atttactgat
     1501 aaaagtatag tgaaattcat ttataaaaaa caatctatta aaactcacta attatgaaaa
     1561 caaaatggat gatggaccca aaccaatcag atatcttaat taagatgaga cattccattg
     1621 tggcttatat gacaggtaca caaaataaat ttgatggata tgttgaaatg aaaaacaatg
     1681 aaattgaagc cgcatcggta gaatttttga tcaatgtaaa taataatgaa accatattag
     1741 aacctaaaaa agcacattta aaattgaata attttttaga taaaaaagaa tatcccatta
     1801 tcagttttaa atctacttct tttgaaaaaa taaataacaa cataaatttt ttgaaaggta
     1861 acctaactat taaaaatata actaaagtaa ttgagcttga tgctgaatac cttggaatca
     1921 aatcatataa cggaacggaa aaagctgcct tcgaaataac cggtgatatt aatagaaaag
     1981 attttggttt agcctttact tcgtttaatc aagccggata cctatcaagc gggaataata
     2041 ttaaactaat tgcaaatttg gaatttacag tttaataaaa aagaaaaatg aaagagaaaa
     2101 ttattgcaaa agccactgat ttgttcctga aacttggatt taagagcgtt accatggatg
     2161 atattgccgg cgaaatgtgt atttcaaaaa agacaattta caaatatttc tgcaacaaag
     2221 aaattctaat tgcagaaaca acggaaattg ttcacaaagc aattcatcaa agcataaata
     2281 ccgtcgtagc taaaaatcat aatgcgattg aagaaaactt tgaaattaga aaaatgttta
     2341 aagaaatgtt taagtcaggt gaagattctc cctcttatca actaaaaaaa cactatccgg
     2401 aaatctacaa aaaagtgatg tctcgagaaa taaacgaatg taatttcact tttaaacaaa
     2461 atattgaaag aggaatcaaa cagggtttgt atcgaaaaga tcttaatccg gatacttatg
     2521 ttggatttta ttacacctta atttttaaca ttaaagagag tactagttca gaaaaagaat
     2581 caagattttt agaattacaa gcattagaat accacatccg ggcaatggcc acaaaagaag
     2641 gagtcttaga actcgaaaaa caattacata actacacttt ataatgaaga cattattttt
     2701 aattccctta ttagtttttg gcttaacggc agaagcccaa gttgaaacat taacgttaaa
     2761 agatgcgtta acctatgctt tggaaaacaa agccgaggct agaaaatctc gattagaaat
     2821 agaaaacagc gaatatcaaa tacaagaaat tcgctctaga gcacttccac aaatttctgc
     2881 aaatggaggt ttaacataca atcctgtttt gcaaacaaat attttagacg gagcaatatt
     2941 tggtcagccc ggacaatctg ttcaagtagc ttttggtcaa aaatggactt caaatgctgg
     3001 ggtttcttta aaccaagcat tatttgatca aacagttttt acgggattaa aagctgcaaa
     3061 atctacaaga gaattttacc aattaaacaa ccaattaact gaagaacaag taatcgaaag
     3121 agttgccaat agctactatt ctgtttacgt tcaacgcgaa cgattaactt tattagatag
     3181 taattatata aattctacaa aagttagaga cattgtaaaa ggacaatatg acaacggatt
     3241 ggcgaaaaaa attgatttgg acagaatcct tgtaaaaata tcaaacattg atacggagcg
     3301 tcaacaagtt aaaaatcaaa ttgaattgca agaaaatgca ttaaaattct acatgggaat
     3361 gcctattgaa aaccaatttg taattcctca aactgaattt gaagtaactc ctatttcgct
     3421 agcagaagtt ccaaatacgg caaacagaag tgaatattta ctcttaaaaa aacaagaaga
     3481 acttttagag tttcaaaaaa ctgctattaa aggagaatat tatcctacac tttcacttgt
     3541 agctggatat aactatttag gacaaggtcc agaaatgcct ctgtttgcaa aacctgccga
     3601 cggcgtttat tggtcggact attctgccat tggattaaat ttaagaatcc ccatttttac
     3661 tggtttcgga actcgttcta gagtgcgaca agcagatatt gacatccaaa tacttcaaga
     3721 agatattaaa gacacgcaac tcgctctcga tttagattat agaaatgcga aagctcaaat
     3781 tgaaaacaac ttaataacca ttaaaaacca aagtgaaaac atgaagttgg cccgtgatat
     3841 tttggataat actaaaaata attaccttca agggttggca actttaactg atttattaga
     3901 ctcccaaaat gaatcactag acgcacaaaa caattataca agagcaattt taaactataa
     3961 aatcgctgaa atcgcattaa ttaaatcaag aggagaactt aaaacactat taaattaaca
     4021 aaaaatgaaa aagacactat taataataat tacaattgta gcttcattag ccctagttgg
     4081 gtatgtttta actaaaaaca aagcagcaaa caaagccaaa acagatattg ttgcagaaaa
     4141 aaatgcagct gtttctgtaa aagtagcaca agtaaagaca gagaatgttt cgttagattt
     4201 tgtcgctaat ggtaactttg ctcctacgca agaattaaat ttttctgctg aaaaatcagg
     4261 aaaagtaatt agtgttttag ttgatgaagg tgactatgtg aaagttggac aaacattagt
     4321 aattgtgcga agtgacgtta taaacgtaaa tgctcaaact gcaaaagctg cttacgaaaa
     4381 tgctaaatca gattatgctc gctatgaaaa tgcctttcaa accggtggag taacaaagca
     4441 acaattagat caagctaaat tacaattaac aaatgctcag tctaacttaa cacaagcaaa
     4501 cattaacgtt ggcgacacta aagtaaaagc tccaatcaaa ggattcgtca acaagagata
     4561 tattgaaccg ggatccattt taacaggaat gcctgcaaca caaatgtttg aaattgtaga
     4621 cgtttctaaa ttaaaattga aagttaccgt aaacgaaagt caggtggcta gtttaaaaat
     4681 tggaaatact gttgacgtaa cttctagtgt tttcccagac aaagtagcta aaggtaaaat
     4741 catttttatt gctccaaaag cagatgaaag tttaaatttt ccagttgaaa ttgagatcac
     4801 aaatagtgct aacagcgaca ttaaagcggg tatgtatgga accgcaactt ttgcaactag
     4861 ccaaaaacaa gatttaaaag ttgttcctcg aaatgctttc gtaggaagtg taagcagcaa
     4921 tcaaatattt gtggttgaaa atggtgttgc taagttgaaa aaagtaaccg ctggtagaat
     4981 tttaggtgat caagttgaaa taattaacgg tttatctgat ggagaaaccg taattgtgac
     5041 aggtcagatt aacttacaag atggaaatgc agttgaaatt ataaaataat taaaagcatt
     5101 acgctgtaag ccttaagctt taaaagaaaa tataaacaca caaagcttaa ggtttatagc
     5161 ctatagcata aagcaatcaa gatgaaatta gcagaaatat ccataaaacg tccctcgtta
     5221 ataatcgtac tctttacgat actaaccctt ggtggactat tcagttatag ccaattgggt
     5281 tatgaattga ttccaaaatt cgaaacaaat gtaattactg tttcaaccat ctatccggga
     5341 gcttccccga gtgaggttga aaacaccata accaaaaagg ttgaagacgg aattgcatcc
     5401 ctagaaaaca tcaaaaaaat cgattcaaaa tcgtacgaaa gtttatctat tgtgtcgata
     5461 acattaacat caaatgcaga cattgacatt tcgatgaacg acgcacaacg taaaatcaat
     5521 gctatcgtga gtgatttacc cgatgatgcc gaaacgccag ctttaaccaa attctcctta
     5581 agtgatttac caattatgac cattggtgcc aacggaaaaa tggacgaagc tactttttat
     5641 gacttaattg ataaaaagat atctcccgtt ttatcacgtg tacaaggtgt ggctcaagta
     5701 aatattattg gtggtcaaga acgcgaaatt caagtaaatt tggatgcagt aaaaatgcaa
     5761 ggttacggac tttcagttcc gcaagtgcaa caaataattt taacttcaaa tctagatttc
     5821 ccaacaggga atattcaaac tagagatcaa aaaatattaa ttcgtttagc cggtaaatat
     5881 aaaaatgttg aagaattaag aaacttagtc atatcttcta ataacggaat tgaagttcgt
     5941 ttaaaggata tcgctgatgt acaagacact caaaaaattg cagagaaaat ctcaagagtg
     6001 gatcaaaaaa gtgcaattat tttgcaaata atcaagcaat cggatgcaaa tgccgttgcg
     6061 gttagtgaag aattaaaaaa tgcaattacc aaacttgaaa cagattataa aaatgctgaa
     6121 ttatctttaa aaattgcaaa agacagtact gattatactt tagaagcagc cgactctgta
     6181 atccacgatt tattgattgc cgtaattttg gttgcctttg taatgttatt tttcttgcac
     6241 agtattcgaa actcattgat tgtaatggtt tcgattccgg catcgttgat tgccactttt
     6301 atcgggatat accttttggg atatacttta aacttaatga gtttactagg actttcactt
     6361 gttgttggta ttctagtaga tgatgccatt gtggtacttg aaaacattta cagacacatg
     6421 gaaatgggta aaaaccgtgt tcgtgccgcc tttgatggta ccgctgaaat tggtggaaca
     6481 gttacctcga ttaccttagt aattgtagtg gtatttttac caattgcaat gagtacaggt
     6541 ttggtttcta atattattac acagttttgt gtaacggtaa ttatatctac cttgttatcc
     6601 ttattgactt cgtttacaat cattccctgg ttgtcttcac gttatggaaa actagaacat
     6661 attgaaggta aaaacttatt tggaagaatc attttaggtt tcgaaagtta tttaacacga
     6721 ttcactaatt gggtttcaaa cttactaaac tggtgcttag atcattacat caaaacaatc
     6781 ggcgttgttt tagtattgtt ttttggagca attgcactta tacctgctgg ctttattggt
     6841 ggtgaattct ttgcatcatc tgatagtggt gagtttttag ttcaaattga aatgccaaaa
     6901 gatgcatcgc tagaacaaac caattttatg actcaaaaag cagaagcttt tttgaaaaat
     6961 caagaatatg tagaaagcca aattacaaca gtaggtcaaa cttctgaagg aatgggtgct
     7021 tctcaagcaa ctgcctacaa agctgagatt gatgttaaaa tgattgatca agcagaacgt
     7081 actgatgatg cttccgttta tgccgcaaaa attaaacgta agttagaaaa agtactagtt
     7141 ggtgcaaaag ttaaaacagt tcctgtcggt attttaggta ctgccgaaaa tgctaaatta
     7201 ggattagttg taacagggcc aaacgttgaa agtgcgatgg cttttgcgaa actagctcta
     7261 gctgaattac gtaccattcc aggaacaacc gaaattaaat taaccgttga agatgggaac
     7321 ccagaaatca atgtacaagt agatcgcgat aaaatggccg ctttaggatt aacacttcaa
     7381 accgttggta tgactatgca aacggcatat agtggaaaca ccgatggtaa atttagagct
     7441 ggagaatacg aatacgacat aaacattaaa tacaatgctt ttgacagaaa aaacattacc
     7501 gatgtgagta atttaatctt cattaacaat gcaggacaac aaattaaatt gtcacaattt
     7561 gcagctatta ctgaaggttc aggtcctagt caattagaac gtcgtgacaa atcggcttct
     7621 gtaacagttc aaggacaaaa tgttggggtt ccaactggaa caattgtaac ccaatggcag
     7681 gaaaaattag acaagttaca aaaaccaacg ggtgtagatt acatttgggg tggtgatcaa
     7741 gaaaatcaaa gtgaaggttt tggtacgtta ggaatcgctt tattagcggc aatcatttta
     7801 gtttacttaa ttatggttgg tctttacaat agttttgctc acccgtttgt agtattattt
     7861 gccattccac tttcgtttat tggagcctta ttagccttgg ctttgaccaa caaaacatta
     7921 aatatattta ccattttagg tatcattatg ttgattggtc tggtctgtaa aaatgctatt
     7981 atgctggtcg attataccaa ccagcgaaga aaagcaggtg aaaccattcg aaaagctttg
     8041 attcaagcga atcatgctcg tttgcgtcca attttgatga caaccattgc aatggtattt
     8101 ggtatgttcc caattgcatt agcttcggga gctggagcag aatggaaaaa tggtttggct
     8161 tgggtaatta tcggtggatt aatttcatcg ctattcctaa ctttaatcat cgttcctgta
     8221 atttatgaaa tcatggaaaa aataattaca aaattctcta aaggagaaaa aatagattat
     8281 gaaaccgaaa tggttgccga ttatgt
//
LOCUS       DAOTCW010000100         8284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_6011_length_8284_cov_36.761575, whole genome shotgun sequence.
ACCESSION   DAOTCW010000100 DAOTCW010000000
VERSION     DAOTCW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6011_length_8284_cov_36.761575"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="P5335_08490"
     CDS             <1..242
                     /locus_tag="P5335_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74954.1"
                     /translation="IDNPQYVIPKSVLVFVYHDNKETNNYFSDQNELEPLLLDQIKTL
                     KKRGRKISKFYESPYIDVYEIVNEPKSSKIDDLIY"
     gene            245..4150
                     /locus_tag="P5335_08495"
     CDS             245..4150
                     /locus_tag="P5335_08495"
                     /inference="COORDINATES: protein motif:HMM:NF021477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2194 domain-containing protein"
                     /protein_id="HRZ74955.1"
                     /translation="MKNRATLNKILKHSLLVFVLIFAYSCADFDAIINPNDTNLFSNE
                     NDNNNLRRYSDADLNLPPLVEYIIDFENPMSVKYSNHLKKLTNYTKIPYKNINIKSWN
                     ANLQIAESTRVLCVLETKKLSDASVNVILKFVSDGGTLFLPFACEDKRFGFMVGMKTT
                     SEYTTDVTSLGIHFTESFIPSLSGLDARSQITHFGMSGDNFDFSKIVVYATAINNKSF
                     PAILENKIGRGRVVNYNTTFEFEKMDRGLLFSGIMLGLEAIPYPIANTNTIFLDDFPA
                     PLYDIKAEPIKSEMNLSIADFVRKSWWPDMKELAKKYQISYTAIPAFDYNVKTNPPFL
                     FSQWDEKKVSQNNKTEPVSSWLMRDCLKNGHELGFHGYNHVSLLKRDWKKQEFIVTAL
                     EGVQKKWKVSSFGDLPVSYVPPSNYIDQMGIKRLKEGMPTLNFMCSLYLGETYDGGNR
                     EYDFDPYHHNMFDYPRISSGFQVDEVSKYNIQSLYLYTGIWNHFVHPDDVYQIPSPFN
                     KSAGEFDLRNPKGLGWKTTKGKKGGLYHELDKYLQEFTSTFPQSRFLNAKQGGMIALD
                     WRASKFNHKAMNGYYFVDELNQGNSLNTSQYWFMYGTTENAENIELQLKTIAVIYSKT
                     KYLDGFLYSIYTNDPSIKIKDYREYREKELDFIFSKIKNEYYTYVAKVNEFEKGTDTA
                     ETSEEDLRIEKAALKQRMLSESKIDYAVWNKYAEYMSWENKGEEVWKMLENHCSKFPT
                     KENVMYSQELDKIIGYPNDLAMEKWINAQLLVNPYDKDLLNSYVANFNTPENAEKIKK
                     ALLALLKVDTGKQSMFNYLQHLIWNEPQAALLELEKIVPSEDYRELATSIVWLYANEK
                     NYQKAYDWSFYSNEIDIANKMEWLFELKQYDALISEYKNHMENNPDDYKTKALMSNYY
                     HDMGKFKEAWVLASELPESKEKEALRKSLNQDVVFQDDFLQQDLLENYPELFLENVTK
                     SLTKANRLKYGNFIESENQLQTNQDRKAALLTRQSYNFYDKKKNLHRIAVTFSEFYPL
                     IRSTVDTIVTDPLLLKAAAEDTPEAISEDNLFRNVYGVEYKYNNPFSYDKFQYWSRIR
                     LEMDNFNDLYMQFGAGINKSFNKNFSSFELNVFPVETAPGHAKEIYQVKTNLYQSMYF
                     IGVLNASLSLESNYYSRSDYNTDFVTDNNIDGSATLRVGWDRGEEKKSKFIPFLETAY
                     QLGSADLSDGYPYWMLKERFFGGAGIGYNYGLETSDFKAKVEAAYFLDDYADEFQRFS
                     GTISYRIFNYFSILGSFELFNQDKFYSNTMNVGLKYNFK"
     gene            complement(4209..5846)
                     /locus_tag="P5335_08500"
     CDS             complement(4209..5846)
                     /locus_tag="P5335_08500"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HRZ74956.1"
                     /translation="MRKLITFLGVLLFMIGSAQTTTKTYTASSDVFSNPERGFYKHTE
                     AHSTGYNLLTQSSLVSYRQNQNITLILRVFYLEEFVNSAISQTYLNNMQTDFNRMRNA
                     GIKCVVRFAYGDDESVSPRDATKVKMLEHIAQLKPVLESNIDVIATFQTGFIGTWGEW
                     YYTSQAEFGGYGYNSSTPLTSANYANRKNVVDAILNALPNSRAIQIRTPKFKQQLYST
                     TTPLPTGQAHTNSSLARIGHHNDCFLSSNSDVGTYSNVTTEYPYLEQETKYLPMGGET
                     CAVFDSRTNCTTALLEMNKFHWSYLNLDYHPSVISGWQTNDCFFEIQNRLGYRFELTS
                     ATLPTQITLNSQLPVTLNLSNKGFSAPFNERNAYIVLKNTVTNVAYPILMDADPRYWL
                     GTKSITENLTLPTDIVPGSYKMYLHLPDVESSIATRPEYAVRFANTNMWESSTGYNDL
                     NHTVIVNQSLSIADNTRLNVVLYPVPADNILNVEMDAAEDYKFTVYNSLGQQIKLNST
                     QNGNKVSFDTQSLSNGIYFIQFENGQTNDSRRFVVRR"
     gene            6441..>8284
                     /locus_tag="P5335_08505"
     CDS             6441..>8284
                     /locus_tag="P5335_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HRZ74957.1"
                     /translation="MKYILIYMKNSIAERIADFLKNFPPFNSLTYLELVKIANEVQVV
                     HVDKNNVLFKVGDKTHSFFYIVKEGAIGLSVTSDAEEALIDKCDEGDILGLRPFFAKD
                     NYLMTAKAREESLLYAIPIEVFKPYVSQNQEVLSFLLESFASNTRNPYDKENKGKLIS
                     ENVIYDEQSSEIQYFQPISYTKKPITANRKDIVKFVAQTMTQSKIGSVIIEENQFPLG
                     IVTDKDLRSKIATGLFPIDVSVDQIMSAPVITVQENISIAETQMMMLKHNIGHLCVTV
                     DGTNKSKIMGIISEHDVVVAQANNPGVLLKQTKRATRSKELKAVRDKLTDLIQNSLDK
                     NIPIQHISNIVGEINIAITKRAVELAIEKMDEQPPTRFAWLNIGSQGRKEQLLITDQD
                     NALVFEDVEDERYDEVKKYFLEMAEKVTKTLNKIGYEYCPAEMMASNSMWCKSLTDWN
                     KQYNSWINTPGEKGILMCSIFFDYDCIYGDEKLVDSITDNIFNQVANNQLFFAYLGTD
                     ALKNPPPLGFFRQFLVENDGEHKDTFDIKSRALMPLVDAARLLTLSLNVKGINNTYHR
                     FKKLAELEPQNAELFENCADAFSLLSKFRAEEGLLNNSNGRYLNLNELS"
BASE COUNT         2931 a         1142 c         1389 g         2822 t
ORIGIN      
        1 taattgataa tcctcagtac gttattccta aaagtgtttt agtatttgta tatcatgata
       61 ataaagaaac caataattat ttttcagatc aaaatgaatt ggagccgcta ttattggatc
      121 agataaaaac attaaagaaa agaggaagaa aaatttctaa attttatgaa agcccataca
      181 ttgatgtata cgaaatagtc aacgaaccca aatcaagtaa aattgatgac ctaatttatt
      241 aagaatgaaa aaccgtgcta ccctaaataa aatcttaaag catagcctat tggtttttgt
      301 tttaattttt gcctatagtt gtgccgattt tgatgcaatt attaatccga atgatactaa
      361 tttattttcg aatgaaaatg acaacaacaa tcttcgcagg tattctgatg ctgatttgaa
      421 tttgccaccc ttggtagaat atattattga ttttgagaat cctatgagtg taaagtatag
      481 taatcactta aaaaaactga ctaattacac caaaattccg tataaaaata ttaatataaa
      541 atcatggaat gcaaatctgc aaatagcaga atctactcgg gttttatgtg ttcttgaaac
      601 aaaaaaactc agtgatgcct cagtaaacgt tatcttaaag tttgtttctg atggtggtac
      661 tctttttctt ccatttgcct gtgaagacaa acgatttggg tttatggttg gaatgaaaac
      721 aacttcagaa tatactactg atgtaacttc acttggaatt catttcacgg aatcatttat
      781 tccatcactt agtggtttag atgctcgatc tcaaatcaca cattttggaa tgagtggtga
      841 taattttgat ttttcaaaaa tagtagtcta tgctaccgct ataaataaca aatcatttcc
      901 agccattctt gaaaacaaaa tagggagggg gagagtagtt aattataata ctactttcga
      961 atttgaaaaa atggaccgtg gattactctt ttcaggtatt atgttaggct tagaagcaat
     1021 tccttaccca atcgctaata ctaatactat tttcttagat gattttcctg caccattata
     1081 tgacattaaa gcggaaccta tcaaatcaga gatgaattta tctattgctg attttgttcg
     1141 aaaatcgtgg tggccggaca tgaaagaatt agctaaaaaa taccaaataa gttatacagc
     1201 aattcctgct tttgattata acgtaaaaac taaccctcct tttttattca gtcaatggga
     1261 tgaaaaaaaa gtaagtcaaa ataacaaaac tgaaccggtg agtagttggt taatgagaga
     1321 ttgtttaaag aatggtcatg aactaggttt tcatggttat aatcacgtat cactcttaaa
     1381 aagagattgg aaaaaacagg aatttattgt aacagctttg gaaggtgtac aaaaaaaatg
     1441 gaaagtaagt agttttggag atttaccggt atcctatgtt cctccttcaa actatattga
     1501 tcaaatggga atcaaacgat taaaagaagg tatgcctacg cttaatttta tgtgtagttt
     1561 atatttaggt gaaacgtatg atggtggaaa tcgagaatat gattttgatc catatcatca
     1621 caatatgttt gattatccta gaatttccag tggttttcaa gtggatgagg tttcaaaata
     1681 taatattcaa tccctttatt tatataccgg aatttggaat cattttgtgc atcctgatga
     1741 tgtttatcaa attccaagtc cattcaacaa aagtgcaggg gaatttgatt taagaaatcc
     1801 aaaagggtta ggctggaaaa ctactaaagg aaaaaaaggc ggtttatacc atgaattgga
     1861 taaatattta caagagttta cctctacatt tccacaatcg aggtttttga atgctaagca
     1921 aggaggtatg attgcattag attggagagc ttcaaaattt aatcataaag caatgaatgg
     1981 ttattatttt gtagatgaac ttaatcaggg aaattcgtta aacacaagcc aatattggtt
     2041 tatgtatgga acaaccgaaa atgcggaaaa cattgaatta caactaaaaa caattgctgt
     2101 aatttattct aaaacaaaat atttagatgg gtttttgtat tcaatttata ccaacgatcc
     2161 atctatcaaa ataaaagatt accgagaata cagagagaaa gaattagatt ttatattcag
     2221 taaaattaag aatgagtatt atacctatgt tgcaaaagta aatgaattcg aaaaagggac
     2281 agatactgcc gaaacgtctg aagaggattt aagaattgaa aaagcagctt taaaacaaag
     2341 aatgttatca gaatcgaaaa ttgattatgc tgtttggaat aaatatgcgg aatacatgtc
     2401 ttgggaaaat aaaggggaag aagtttggaa aatgcttgaa aatcattgtt ctaaatttcc
     2461 aaccaaagaa aatgtaatgt attcacagga attggataaa ataattggtt atccaaacga
     2521 tttagctatg gaaaaatgga taaatgcaca attgttagta aatccgtacg ataaggattt
     2581 attgaatagt tatgtcgcta actttaatac accggaaaat gcagaaaaaa ttaaaaaagc
     2641 attattagct cttttaaaag tagataccgg aaaacaaagt atgtttaatt atttacagca
     2701 tttaatttgg aatgaaccgc aagccgcttt actggaatta gaaaaaattg tgccgtcaga
     2761 agattataga gaattagcta ctagtattgt ttggttatac gccaatgaaa agaattatca
     2821 aaaagcgtat gattggtctt tctatagcaa tgaaattgat attgcgaata aaatggaatg
     2881 gctttttgaa ttaaaacaat atgatgcttt gataagtgaa tataaaaatc acatggaaaa
     2941 taatccggat gattataaaa ccaaagcgtt gatgagtaat tattaccatg atatgggtaa
     3001 attcaaagaa gcatgggttt tagccagtga attaccggaa tcaaaagaaa aagaagccct
     3061 tcgtaaatca ttaaaccaag atgtggtttt tcaagatgac tttttacaac aagatttatt
     3121 agagaattat cccgaattat ttttagaaaa tgtaactaag tcgttaacta aagctaatcg
     3181 tttgaaatat ggtaatttta ttgaatcaga aaatcaattg caaaccaatc aagatagaaa
     3241 agcggcattg ttgaccagac aatcgtataa tttctacgat aagaaaaaga atttgcatcg
     3301 tattgccgtt actttttcag aattttatcc cttaatccgc tcaacggtgg atacaattgt
     3361 aaccgatccg ttattgctta aagcggctgc agaggacact cctgaggcta tttctgaaga
     3421 taacttattc agaaatgtat atggtgttga atacaaatat aataacccat tttcgtatga
     3481 taaattccaa tattggtcta gaatacgact tgaaatggat aattttaatg atctatatat
     3541 gcaatttggt gccggtatta ataaatcatt taataaaaat ttcagttctt tcgaattgaa
     3601 tgtttttcct gtggaaacag ctccaggaca cgcgaaggaa atatatcaag taaaaaccaa
     3661 tctttatcaa agtatgtact ttattggagt tttaaatgcc agcttgtctt tagaatctaa
     3721 ttattatagt agaagtgatt acaatactga ttttgtgacg gataataata ttgatggttc
     3781 agctacttta agagttggat gggatagggg tgaagagaaa aaatcaaaat ttattccgtt
     3841 tttagaaacg gcctatcaac tgggttcagc agatttaagt gatggttatc cttattggat
     3901 gttaaaagaa cgattttttg gaggagcagg aattggttat aattacggac tagaaaccag
     3961 cgatttcaaa gcaaaggttg aagcggcata ttttttagat gattatgcag atgaattcca
     4021 acgtttctct ggaacaattt cctatagaat ttttaattat ttttctattt tgggaagttt
     4081 tgaattattt aatcaagata aattttattc caataccatg aatgttggat taaaatataa
     4141 tttcaaataa aaaaaatgat gaagtacaaa agtgcttcat catttttaga atcaaacatg
     4201 aaattttttt atcttcttac aacaaatctt ctggagtcat tagtttgacc attttcaaat
     4261 tgaataaagt aaataccatt acttaaactt tgtgtatcaa agcttacttt gtttccgttt
     4321 tgagttgaat tcaattttat ttgttgacca agtgaattgt aaactgtaaa tttataatct
     4381 tcagcagcat ccatttctac atttaaaata ttatctgccg gaactggata taatacaaca
     4441 tttaaacgag tattgtcagc aatactaagt gattgattca caattactgt atgatttaaa
     4501 tcattatatc ctgttgaaga ttcccacata tttgtattcg caaatcgaac ggcatattcc
     4561 ggacgggtag ctattgaact ttcaacatcc ggtaaatgta agtacatctt ataacttccc
     4621 ggaactatat ctgttggtaa agttaagttt tcagtaattg attttgttcc taaccaataa
     4681 cgtgggtcag catccattaa gatgggataa gcaacgttag ttaccgtatt tttcaacaca
     4741 atataggcat ttcgttcatt aaacggagct gaaaaacctt tatttgataa atttaatgta
     4801 actggtaatt gactgtttaa tgtaatttgt gttggcaaag tagcactagt caattcaaaa
     4861 cggtaaccta atctgttttg aatttcaaaa aaacaatcat tggtttgcca tccgctaata
     4921 acacttggat gataatctaa attcaaatag gaccaatgga atttattcat ttctaataag
     4981 gcagttgtac aatttgttct tgagtcaaaa actgcacaag tttcaccacc cattggtaaa
     5041 tattttgttt cttgttccaa ataaggatat tcagttgtca catttgaata tgttccaaca
     5101 tcactgttac tggataaaaa acaatcgtta tgatgtccaa ttctggccaa agaggaattt
     5161 gtatgagctt gaccggtagg caaaggagtt gttgtactat ataattgttg tttaaacttt
     5221 ggagttctaa tttggattgc tcttgaatta ggaagtgcat tcaaaattgc atcaacaaca
     5281 ttctttctat tggcataatt tgcactagtt aaaggagtag aactattgta tccataacct
     5341 ccaaattcgg cttgtgaagt gtaataccat tctccccaag ttccaataaa tccggtttga
     5401 aaagtggcaa ttacatcaat atttgattct aaaactggtt ttaattgagc aatgtgttcc
     5461 aacattttaa ctttagttgc atctcttggg ctaacacttt catcatctcc ataagcaaaa
     5521 cggacaacac atttgattcc ggcattacgc attcggttaa aatcagtttg catattgttc
     5581 aaataggttt gcgaaatagc tgaatttaca aactcttcca aataaaaaac acgtaatata
     5641 agtgttatgt tttgattttg tctgtaacta actaaggaag attgtgtcag caggttgtaa
     5701 cctgtagaat gggcttccgt atgtttgtag aaacctcgtt caggattaga aaatacgtca
     5761 cttgaagcag tatacgtttt agtcgtcgtt tgtgcacttc caatcatgaa cagaagaaca
     5821 ccaagaaaag taattaattt tctcatagta gtaggtttgt ttttgctaaa tttgggtcta
     5881 gcgggttatc ggactagttt ttttactctt aactatgttg tggctcatag ttttatgttc
     5941 caaatataga ttaaaagtgc tttttaaccg ttgaaataca taataaaatt tcacataaaa
     6001 tcgatgaaat acatgttttt ttatttagtt taaatattat cttacattta gttaattttt
     6061 gctgtacgta tttttttatt tttatttttt tattgaaatc attttttttc attattaggc
     6121 aatattgtaa actttgaaat tttatatttt tattttctat taaaacttta aaatataatt
     6181 cataatccat tgactcattt tagatttttt aaataattta cctgactaaa tataactata
     6241 atttatatta tgtaaaatag aatttatcta tttatactat atatccttct gttataatct
     6301 aggtatgctt acatatcgta cgtaacatat tcaaatcaat gtaagttaag tggtaattaa
     6361 ttataagtac tattgttatc taataagtta ataaattcaa attaatgaat atcaattaga
     6421 atcataattt atgtaacttt atgaaatata ttcttatcta tatgaaaaac tctattgctg
     6481 aacgtatagc cgattttttg aaaaattttc caccattcaa cagtttgact tatcttgaat
     6541 tggtcaaaat tgccaacgaa gttcaagttg ttcatgttga taaaaacaat gtacttttta
     6601 aagttggcga taaaacacat tcttttttct atattgttaa agaaggtgcc attggtcttt
     6661 ctgtaacttc tgatgcagaa gaagctttaa ttgataaatg tgacgaaggt gatattttag
     6721 gtttacgtcc attttttgcg aaagataatt atctaatgac agcaaaagct agggaagaaa
     6781 gtttattata tgctattcca attgaggttt ttaagcctta tgtatcacaa aatcaagaag
     6841 ttttaagttt tttattggaa agttttgctt ctaatacacg taacccttac gataaagaaa
     6901 ataaagggaa attaatttct gagaatgtta tttatgatga gcaaagttct gaaattcaat
     6961 attttcaacc catttcatat actaaaaaac ccattacagc caaccgaaaa gatattgtaa
     7021 agtttgttgc tcaaacaatg actcaaagta aaattgggag cgtaattatt gaagaaaacc
     7081 aatttcccct tggtattgtg actgacaaag atttgcgatc aaaaattgca acaggtcttt
     7141 ttcctattga tgtttcagtt gatcagatta tgtcggctcc ggtaattact gtccaggaaa
     7201 atatttcaat tgctgaaact caaatgatga tgttaaaaca caatattggg cacttatgtg
     7261 ttaccgttga tggtacaaat aaatctaaaa tcatgggaat tatttccgaa catgatgttg
     7321 ttgttgctca ggcaaataac cccggagttt tattaaaaca aactaagcgt gctacccgtt
     7381 ccaaagaatt aaaagcagtt cgcgataaat taactgattt aattcaaaat tcattagata
     7441 aaaatattcc tatccaacac atttccaaca ttgtaggcga aattaatatt gccattacta
     7501 aaagagcagt ggagctagct attgaaaaaa tggatgaaca accccctact cgatttgctt
     7561 ggttaaatat tggaagtcaa ggaaggaaag aacaattact cataactgat caggataatg
     7621 ctttagtttt tgaggatgtt gaggatgaac gctatgatga agtgaaaaaa tattttttag
     7681 aaatggccga aaaagtgact aaaacattga ataaaattgg ctatgaatat tgccctgcag
     7741 aaatgatggc tagtaattca atgtggtgta aatcattgac agactggaat aaacaatata
     7801 atagttggat taatactccc ggtgagaaag gaattttaat gtgttctatt ttttttgatt
     7861 atgattgtat ttacggtgat gaaaaactag tagattctat cacggataat atttttaatc
     7921 aggtagccaa caaccaattg ttttttgctt atttaggtac agatgcatta aaaaatcctc
     7981 ctcctttagg ctttttcaga cagtttttag ttgaaaatga tggtgaacat aaagatacgt
     8041 ttgatataaa aagcagggct ttgatgccgt tggttgatgc cgctcgttta ttaacattaa
     8101 gtttaaatgt aaaaggtata aataacacct atcatcggtt taaaaaatta gcggaattag
     8161 aacctcaaaa tgccgaactt tttgaaaatt gtgcagatgc tttttcttta ttatctaaat
     8221 ttagagcaga agaaggtttg ttgaataatt caaatggcag gtatttaaat ttaaatgagt
     8281 tatc
//
LOCUS       DAOTCW010000101         8278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_6020_length_8278_cov_47.714095, whole genome shotgun sequence.
ACCESSION   DAOTCW010000101 DAOTCW010000000
VERSION     DAOTCW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6020_length_8278_cov_47.714095"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..392
                     /locus_tag="P5335_08510"
     CDS             54..392
                     /locus_tag="P5335_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ74958.1"
                     /translation="MKKNYIYTLLTVLFFTLTVSAQEVKSQQPNKPQETTIEGLSFYP
                     NPVSNGKVYITSKSTLDKEVIIFDILGKIVLQSTITSKELNVSSLSPGVYMIKIKEGD
                     ATATRKLIIK"
     gene            497..2206
                     /locus_tag="P5335_08515"
     CDS             497..2206
                     /locus_tag="P5335_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ74959.1"
                     /translation="MKKLYTLLFLAVSSFGFAQTFYSENMGTPSGTTAIAANVFQNTA
                     PIVYSGDGDVRATAVSNGYIGASGAGNVFLTGTAGKFFRIDGLNTAAYSSANLQLKFG
                     YLTNSTATQLLVEQSIDNGTTWTPITFTQNANTSWNLITITGGIQSSATLSLKFTQPA
                     TAQMRIDDVSLTNFSASCTLVLGADSTLCNASTLALDTYNATIPFTGAGNATYTIETT
                     SGTVAGDNPSSVAEGNIVIEGITEGTNITVTVTGGTCNFSRDIFSAECKPVNALPHGD
                     SFDYAVDTNLGSTQKWTNVNSGDNVLAVAGSLNYTGINSVGNSVSFSGSGIDPYTPFT
                     STTSGAIYTGFLMSVTDYSNVTNDLVETAFAVVTDDIRGFKARIFIKKNGAQFQLGCT
                     SATTATPTIYGATLYNVGDVVYVVVGYDFTANELKLWLNPTVAGFTAATPATIVETPA
                     TAFANLGGFILRQDGATSTPTMTIDELRVATSVGGLLSVKSNAISGLKVYPNPISNGV
                     LYISTEANTERTVQIFDLLGKQVLNITTSNEEVNVSTLNTGLYVVKITEGGNTATRKL
                     VIK"
     gene            2278..3258
                     /locus_tag="P5335_08520"
     CDS             2278..3258
                     /locus_tag="P5335_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HRZ74960.1"
                     /translation="MNPYPDIFSIGSQKEFEKITLKVFRHQYEHNQVYQDFCSLLKKD
                     KTNVKSLEEIPFLPIQFFKSHAVLSSSKPIQETFTSSGTSGMVSSKHLVTDLSLYEQS
                     FRKAFSQFYGNSEDYVILALLPSYLEREGSSLIYMVNDLIESSNHPESGFYLHNYDEF
                     VQQLIKIDSSGQNVLLIGVTYALLDVVEKQTFQLKNTIIMETGGMKGKRKEIIREELH
                     EILGNGFGVSAIHSEYGMTELLSQAYSLGNGIFECPPWMQILIRDTEDALTYVDFGKT
                     GGINVIDLANINSCSFIATQDLGKKNPNHSFEVLGRFDSSDIRGCNLMVI"
     gene            complement(3251..4561)
                     /gene="tyrS"
                     /locus_tag="P5335_08525"
     CDS             complement(3251..4561)
                     /gene="tyrS"
                     /locus_tag="P5335_08525"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRZ74961.1"
                     /translation="MKNLVEEFRWRGLFHDMMPGTEEQLLKESTTAYIGFDPTADSLH
                     IGSMVQIILLIHLKNFGHKPIALVGGATGMIGDPSGKSDERNLLDEATLTKNVEGIKK
                     VLSRFLDFNSTEANAPVLVNNYDWMKDFTFIEFARDIGKRITVNYMMAKDSVKKRLSG
                     EVGEGMSFTEFTYQLIQGYDFYYLHKHYNCLLQMGGSDQWGNITTGTELVRRMNVNSS
                     EEGAKAFALTTPLITKADGSKFGKSEGGNVWLTADKTSPYKFYQFWFNSTDADAEKYI
                     KIFTFLEKEIIEELIEKHQQEPHLRLLQKRLAEEITVLVHSRDELEKAVKASNILFGN
                     STSEELKQLDNQTFLDVFDGIAQAEISKTDLENGLTIVEALAGKSGFMSSNGEARRAL
                     KENSISVNREKVTEEFLITKNDLINDEFVLLQRGKKTYFILRFI"
     gene            4703..5698
                     /locus_tag="P5335_08530"
     CDS             4703..5698
                     /locus_tag="P5335_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRZ74962.1"
                     /translation="MNLVTGGTGLVGAHLLLQLLEKGEKVRALFRSEKSIQKTKSLFI
                     QFKQSELFEQIEWIKGDIIDIPTLEPAFKNVVHVYHCAALISFDTADEKLLRKINIEG
                     TANVVNCALAFSVKKICCVSSIAALGDLAEKKVLITEETEWNPEKPHSDYAISKYGAE
                     MEIWRAEQEGLKCVVINPGVILGSGFWNSGSGEIFTKVSKGIPFYTKGSTGFVAVEDV
                     VTVMYQLMKSDVSGERFTLISENVNFEKLVCWIAKELNVKGPSFYAKPWMTEIGWRLD
                     WFISTFFFQKRKLSKSSAKSLHSTDLISNEKIKKAIYFEFQPIQEYIIKISPYFF"
     gene            complement(5679..6128)
                     /locus_tag="P5335_08535"
     CDS             complement(5679..6128)
                     /locus_tag="P5335_08535"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HRZ74963.1"
                     /translation="MKIKHALFFFSFFFFACQKEKVKVPEKLIEEETMVSIFYDLSLL
                     DAIRSNNSILLEENKIDPYTYIYKKYKIDSLQFAQNNIYYAYDLKRYKSMYERVEKRL
                     EEEKKIADSTVLKPGKIEEPPSTQKDTIQTRIKEGFLKNKELRKNKD"
     gene            complement(6132..7469)
                     /locus_tag="P5335_08540"
     CDS             complement(6132..7469)
                     /locus_tag="P5335_08540"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRZ74964.1"
                     /translation="MKRILIKNAKIVNEGKIFEGDLLIENEIIIEISDSISAKTDYKV
                     IDAEGNYLIPGAIDDQVHFREPGLTHKGDIGSESRAAVAGGITSFIEQPNTVPNAVTQ
                     ELLEQKYQIAAETSHANYSFMMGGTNDNLDEILKTNPKNVAGVKLFLGSSTGNMLVDK
                     EDVLEKIFSSTKMLIAVHCEDETTIKNNLAKYKEKYGDDIPVKFHHLIRSEEACYLSS
                     SKAIALAKKTGARLHVFHLSTAKEMELFTNKIPIEEKQITAEVCIHHLWFTNADYETK
                     GNLIKWNPAIKTETDRAALWEALLDDRIDVIATDHAPHTLEEKNQSYTKAPSGGPLVQ
                     HAVVAMFEAFHQGKISIEKIVEKMAHNPAKIFKIEKRGFIKEGFYADLVLINPAMPWS
                     VKKENILYKCGWSPLEGTTFKSRITHTFVNGQLVYQNAKVNEAKAGKRLLFNR"
     gene            complement(7483..8208)
                     /locus_tag="P5335_08545"
     CDS             complement(7483..8208)
                     /locus_tag="P5335_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRZ74965.1"
                     /translation="MNNGLVIIPTYNEIENVESIIRAVFALNKKFHILIVDDNSPDKT
                     FEKVKELQNEFPNQLFLKVGTKKAGLGTAYVLGFKWALQQQYDYIFEMDADFSHNPSD
                     LEKLLEACSDGGADMAIGSRYIRGVNVVNWPLSRVLLSYFASVYVDVITGMKIHDATA
                     GFICYKKEVLQNIDLDKIKFVGYAFQIEMKYRAFCKGFKIVEVPIIFTDRTMGQSKMS
                     NGIIKEAVFGVISLRIKKLFNRL"
BASE COUNT         2662 a         1403 c         1320 g         2893 t
ORIGIN      
        1 tttggtatgg taattgaaat attttattat ctttgttata ccaaatcatt gaaatgaaaa
       61 aaaattacat ctatactctt ttaacggttt tgttcttcac cctgacagtt tctgcccagg
      121 aagttaagtc gcaacaacct aataaacctc aggaaactac cattgagggg ttaagttttt
      181 accctaaccc ggtaagtaac gggaaagttt atattacttc taaatcaact ttggataaag
      241 aagttattat ttttgacatt ttaggaaaaa ttgtattaca aagcacaatt acctcaaaag
      301 aattaaacgt ttcatcactt tctcccggcg tttatatgat taaaatcaaa gaaggagacg
      361 caacagctac aagaaaactg attattaaat agttaggttt tcattatttt acttttgttt
      421 ttacaatttt ttaataaggt tatgttttgt aacttattta ttgttactat ctttgtacaa
      481 taaattttat aaaaaaatga aaaaacttta cactttatta tttttggcag tatctagttt
      541 cggttttgcc caaacttttt actctgaaaa catgggaacc cctagtggta ctactgctat
      601 tgcagcaaat gttttccaaa acactgcacc aattgtttat tcaggagatg gagatgttag
      661 agcaactgca gtttctaatg gttacattgg agcttcagga gctggaaatg tatttttaac
      721 aggaactgct ggaaaatttt ttagaattga tgggttaaat acggctgctt atagtagtgc
      781 aaaccttcaa ttgaaatttg gttatttaac gaattctact gctactcaat tacttgtaga
      841 acaaagtatt gacaacggaa cgacttggac accaatcaca tttactcaaa acgcaaacac
      901 ttcttggaat ttaattacta ttacaggtgg aatccaatca tctgctactt taagtttaaa
      961 attcacgcaa ccggcaaccg ctcaaatgag aattgacgat gttagtttaa ctaatttttc
     1021 agcttcttgt acacttgtgt taggtgctga ctcaacttta tgtaatgcgt caactttagc
     1081 attggacact tataatgcaa ccattccttt cacaggggcc ggaaatgcaa cttatactat
     1141 cgaaactact agtggaacag ttgcgggaga taatccatca agtgttgcag aaggtaacat
     1201 cgtgatagaa ggaattacag aaggaacaaa cattactgta acggtaactg gtggaacttg
     1261 taatttttca agagatattt tttctgcaga atgtaaacct gtaaatgctc ttccacatgg
     1321 agatagtttt gattatgcag ttgatactaa tcttggatca actcaaaaat ggacaaatgt
     1381 taactctggt gacaacgtat tagctgtggc tggaagttta aactacacag gtattaattc
     1441 tgttggtaat tcagtttcat tttccggttc aggaattgat ccttatactc cttttacatc
     1501 aactacttcg ggagctatct atactggatt cttaatgagt gtaacagatt attcaaatgt
     1561 aacaaatgat ttagttgaaa cagcttttgc agttgttaca gatgatatca gaggttttaa
     1621 agctagaatc tttattaaaa agaatggtgc tcaattccaa ttaggttgta catcagcaac
     1681 aacagcaaca ccaactattt atggagctac tctatataat gtaggtgatg tagtttatgt
     1741 ggttgtagga tacgatttta ctgctaacga acttaaatta tggttaaacc caacagttgc
     1801 aggtttcaca gcagctactc cagctacaat tgttgagact ccagctacag catttgctaa
     1861 tttaggtggt ttcattttaa gacaagatgg agcaacaagt acaccaacca tgacaattga
     1921 tgaacttaga gttgctactt cagttggtgg attattaagt gtaaaatcaa atgctatttc
     1981 aggtttaaaa gtatatccta atcctatttc aaacggtgtt ttatatatct caacagaggc
     2041 taatactgaa agaacagtac aaatttttga cttattaggc aaacaagtat taaacattac
     2101 aacttctaat gaagaagtaa atgtttctac tttaaatacc ggtctttatg ttgtaaaaat
     2161 cactgaaggt ggaaatacag ctactagaaa attagttatc aaataagtaa ctttttcaaa
     2221 atattaaaaa gcatcaacga aagttggtgc tttttttatt gctatttttg caacgaaatg
     2281 aatccttacc ccgacatatt ttccattgga agtcaaaaag aatttgagaa aataacactc
     2341 aaagtttttc gacatcaata tgaacacaat caagtatacc aagatttttg ttccctatta
     2401 aaaaaagaca aaaccaatgt aaaatcattg gaagaaatcc cttttcttcc tatccagttt
     2461 tttaaaagcc atgccgtttt gagttcatca aaacccattc aagaaacatt taccagtagc
     2521 ggaactagtg gaatggtttc aagcaaacac ttggttaccg acttgagttt atatgaacaa
     2581 agttttcgaa aagcattttc tcaattctac ggaaacagtg aagattatgt tatattggct
     2641 cttcttcctt cctatttaga acgagaaggt tcttctttaa tttatatggt aaatgatttg
     2701 atagaaagct caaatcatcc cgaaagtggg ttttacctcc acaattatga tgaatttgtt
     2761 cagcaattaa ttaaaattga ttcttcaggt caaaatgtac ttttaattgg cgtgacttat
     2821 gcacttttgg atgtagttga aaaacaaact tttcaattaa aaaataccat tattatggaa
     2881 accggtggca tgaaaggtaa acgaaaagaa atcatccgcg aagaattaca cgaaatctta
     2941 ggcaacggtt ttggtgtttc agctattcat tctgaatatg gtatgactga gttattatca
     3001 caagcctatt ccttgggaaa tggcattttt gaatgtcctc cttggatgca aatcctcatt
     3061 cgagatacag aagatgctct gacttatgtt gactttggaa agacaggcgg tataaatgtg
     3121 attgatttag ccaatatcaa ttcttgttcg tttattgcca cacaagattt gggtaaaaaa
     3181 aatcccaacc attcgtttga agtgttggga cgttttgatt ccagcgatat tcggggttgt
     3241 aatttgatgg ttatataaac cgcaaaataa aatacgtttt cttcccacgt tgcaatagca
     3301 caaattcgtc gttaattaag tcatttttag taattaaaaa ttcttctgta actttttcac
     3361 ggtttacaga aatggaattt tcttttaatg ctcttctcgc ttccccgtta gaagacataa
     3421 atcctgattt accggctaat gcctcaacaa ttgtcaatcc attttctaaa tcagttttag
     3481 aaatttcagc ctgagcaatt ccgtcaaaaa catctaaaaa cgtttggtta tccaattgtt
     3541 ttaactcttc agaagtggaa tttccaaata aaatattgga tgctttcacc gctttttcaa
     3601 gttcatctct ggaatgaact aaaactgtga tttcttctgc taatcgcttt tgcaacaaac
     3661 gtaaatgggg ttcttgctga tgtttttcaa tcaattcttc tattatttct ttctctaaaa
     3721 aagtaaagat ttttatatat ttttcagcat ccgcatcagt tgaattaaac caaaattgat
     3781 aaaatttata cggcgatgtt ttatcagcgg ttaaccaaac gtttcctcct tctgatttac
     3841 caaatttgga cccatctgct ttcgttatta atggagtcgt taaggcaaaa gcttttgccc
     3901 cttcttcaga agaatttaca ttcattcttc taactaattc cgtcccggtg gtaatatttc
     3961 cccattggtc tgaacctccc atttgcaaca aacaattgta atgtttatgt aaataataga
     4021 aatcatatcc ctgaattaac tgataagtaa attctgtaaa ggacattcct tccccgacct
     4081 ctccgctcaa tcttttctta acagaatctt tagccatcat ataatttact gtgatgcgtt
     4141 ttccaatatc acgagcaaat tcaataaagg taaagtcttt catccaatca taattattga
     4201 ctaaaacggg agcattggct tctgtcgaat taaaatccaa aaaacgcgat aatacttttt
     4261 ttattccttc aacattttta gttaacgttg cttcatctaa taaatttctt tcgtctgatt
     4321 ttcccgatgg atcaccaatc attccagttg caccaccaac taatgcaatt ggtttgtgtc
     4381 caaaattttt caaatgaatc aacaagatga tttgtaccat actgccgata tgtaaggaat
     4441 cggctgttgg atcaaatcct atataagcag tcgtagattc ttttaataat tgctcttctg
     4501 ttcccggcat catatcgtga aacaatcctc tccagcgaaa ttcttctact aaatttttca
     4561 tgcgtatatt ttattgattt ttataggtga cacggaatcg cttattttct ttttagcaat
     4621 ttcatctaaa aataattttg gcaaagatag ttttaattcg ataatttgaa aatgtagaac
     4681 ggaaacaatt atatttgttt ttatgaatct agttacaggt ggaaccggtt tagtgggagc
     4741 acacttattg ctccaattat tagaaaaagg cgaaaaagtt cgggctttat ttcgttctga
     4801 aaagagcata caaaaaacaa aatcgctttt tattcaattt aaacaatctg aacttttcga
     4861 acaaatagaa tggattaaag gagatattat tgacattcct acgttagaac ctgcttttaa
     4921 aaatgttgta catgtttacc attgtgctgc cttaatttcc tttgatacag cagacgaaaa
     4981 actattgcgt aaaataaaca tagaaggaac ggcaaatgtt gtgaattgtg cgttagcttt
     5041 tagtgtaaaa aaaatttgct gcgtcagttc catcgctgct ttaggtgatt tagcagaaaa
     5101 gaaagtttta attacggaag aaactgaatg gaatcctgaa aaaccacaca gtgattatgc
     5161 tatttctaag tacggagctg aaatggagat ttggagagca gaacaagaag ggttaaaatg
     5221 tgttgttata aatccgggag taattttagg ctccggtttt tggaattcag gtagtgggga
     5281 aatctttacg aaagtgagta aaggaattcc gttttatacc aaaggatcaa ccggatttgt
     5341 ggcagttgaa gatgttgtaa cagttatgta tcaattaatg aaaagtgatg tttccggtga
     5401 acgatttact ttaatttctg aaaatgtaaa ctttgaaaaa ttggtttgtt ggattgctaa
     5461 agaattaaat gtaaaagggc cttcttttta tgcaaaacca tggatgactg aaattgggtg
     5521 gcgattggat tggtttattt caacattctt ttttcaaaaa agaaaactat ctaaatcatc
     5581 cgctaaatca ttacattcaa ctgatttaat ttcgaatgaa aaaataaaaa aggctatcta
     5641 ttttgaattt caacctattc aagaatacat tataaaaatc agtccttatt ttttctaagt
     5701 tctttatttt ttaaaaatcc ctcttttatt ctggtttgaa tagtatcttt ttgtgtagaa
     5761 ggtggttctt ctatttttcc gggttttaaa actgtactat cagcaatctt tttttcttct
     5821 tccagtcgtt tttcaactcg ttcatacatg cttttataac gctttaaatc ataagcataa
     5881 taaatattgt tttgagcaaa ttgcaaactg tcaattttgt atttcttgta gatatacgtg
     5941 tagggatcta ttttattttc ttccaacaaa atagaattat ttgatcgaat tgcatccaac
     6001 aaacttaaat catagaaaat agaaaccatg gtttcttcct ctatcagttt ttcaggaact
     6061 tttacttttt ctttctgaca agcaaagaaa aagaaactaa aaaaaaacaa agcgtgtttt
     6121 attttcatac tctatcgatt aaaaagtagt cgcttaccgg ctttagcttc gtttactttt
     6181 gcattttgat acaccaattg accattgaca aaagtatgtg taattcttga tttgaatgtg
     6241 gttccttcta aaggagacca accacattta tacaatatat tttctttttt tacactccat
     6301 ggcatggcag gatttattag taccaaatca gcataaaaac cttcttttat aaaacctctt
     6361 ttctcaattt tgaatatctt agccggatta tgagccattt tttcgactat tttttctatt
     6421 gaaatttttc cctgatgaaa agcctcaaac atggctacta cagcatgctg aactagtgga
     6481 cctccggaag gtgctttggt ataggattgg tttttttctt ccaaagtatg tggtgcgtga
     6541 tctgttgcta taacatctat acgatcatct aacaaggctt cccataaagc tgcccgatca
     6601 gtttctgttt taatggcagg attccatttt attaaatttc cttttgtttc ataatctgca
     6661 ttggtaaacc acaaatgatg aatacaaact tcggcggtga tttgtttctc ctctattgga
     6721 attttattgg taaataattc catttctttc gcggtagaaa gatgaaaaac atgcaatctt
     6781 gctcctgttt tctttgctaa tgctattgct ttggaagaag acaaataaca agcttcctcg
     6841 ctacgtatca aatggtgaaa cttgaccgga atatcgtcac catatttctc tttatacttg
     6901 gctaaattat ttttaatggt ggtttcatct tcgcaatgca ccgcaatcag catttttgta
     6961 gaggaaaata ttttttctaa aacatcttct ttatccacta acatatttcc ggtagaagaa
     7021 cctaaaaaaa gtttaactcc ggcaacattt tttggattgg tttttaaaat ttcatccaaa
     7081 ttatcgttag ttccgcccat cataaaagaa taattagcat gggaagtctc ggctgctatt
     7141 tgatattttt gttctaataa ttcttgagta actgcattcg gaacagtatt aggctgttca
     7201 ataaaagagg tgattcctcc tgcaactgcc gctcttgatt ctgaaccaat atcaccctta
     7261 tgagtgagtc cgggctcacg aaaatgaact tggtcatcaa ttgcccctgg aataaggtaa
     7321 tttccttccg catcaattac tttatagtca gttttagcac taatactatc tgaaatttca
     7381 ataattattt cgttttcaat gagtaaatct ccttcaaaaa ttttaccttc attgacaatt
     7441 ttagcgtttt ttattaaaat ccttttcatt tattcttttc aattataatc ggttaaacag
     7501 tttttttatt cgtaaagaaa ttacaccaaa aactgcttct ttaataattc cattgctcat
     7561 tttagattgt cccattgttc tgtctgtaaa aataatgggg acttcaacaa ttttaaatcc
     7621 tttgcaaaag gcacggtatt tcatttcaat ttgaaatgca taacccacaa atttaatttt
     7681 atccaaatct atgttttgaa gcacttcttt tttgtagcaa ataaatccgg ctgttgcatc
     7741 atgaattttc attccggtaa taacatcaac ataaaccgaa gcaaaatagg acagaagaac
     7801 tcgactcaaa ggccagttaa caacgtttac acctcgaata tagcgagaac caattgccat
     7861 atcggcacct ccatccgaac aagcttcaag caatttttct aaatctgaag gattatgtga
     7921 aaaatcggca tccatttcga aaatgtaatc gtattgttgt tgtaatgccc atttaaatcc
     7981 aagcacataa gcggtaccca atccggcttt tttggttcca acttttaaga aaagttgatt
     8041 gggaaattcg ttttgaagtt ctttaacttt ttcaaatgtt ttgtctggag aattatcgtc
     8101 tacaatgaga atatgaaact ttttattcag tgcaaataca gcccgaataa tactttctac
     8161 attttcaatt tcattgtagg ttggaattat aactaatccg ttattcataa atactacaaa
     8221 atttcaaagc aaatgtaaac tttttataaa tgatgattca taataatatt ataataaa
//
LOCUS       DAOTCW010000102         8211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_6079_length_8211_cov_45.014223, whole genome shotgun sequence.
ACCESSION   DAOTCW010000102 DAOTCW010000000
VERSION     DAOTCW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8211
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6079_length_8211_cov_45.014223"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="P5335_08550"
     CDS             complement(<1..530)
                     /locus_tag="P5335_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRZ74966.1"
                     /translation="MITKEKILATAKETILSESKAISKLSELLTDDLADCVRLIYESK
                     GRLIVTGIGKSAIIAQKIVATLNSTGTPSLFLHASEAVHGDLGMVQPNDIIICISKSG
                     NSPEIKILVPILNRFGNTLIAMTGNLSSFLAKNSTYVLDTTVEKEACPNNLAPTNSTT
                     AQLVMGDCLAVCLMEI"
     gene            616..2811
                     /gene="recQ"
                     /locus_tag="P5335_08555"
     CDS             616..2811
                     /gene="recQ"
                     /locus_tag="P5335_08555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRZ74967.1"
                     /translation="MNPNEIDIHKELKKYFGFDHFKGLQEQVIKSILNKENTFVIMPT
                     GGGKSLCYQLPALIQDGTAIVVSPLIALMKNQVDAIRSVSSEDGIAHVLNSSLTKTEI
                     NQVKKDIVSGITKLLYVAPESLAKEEYVDFLKSVPISFIAIDEAHCISEWGHDFRPEY
                     RNLKNIIKHLGNVPVIGLTATATPKVQEDILKNLDMSDATTYKASFNRPNLFYEIRPK
                     TKNIESDIIKFIKHNKGKSGIIYCLSRKKVEEIAQVLQVNGISAVPYHAGLDAKTRAK
                     HQDMFLMEDVEVVVATIAFGMGIDKPDVRFVIHHDIPKSLESYYQETGRAGRDGGEGH
                     CMAYYSYKDVEKLEKFMSGKPVAEQEIGFALLQEVVAYAETSMSRRKFLLHYFGEEFD
                     NETGEGGDMDDNVRNPKAKAEAKDDVVKVLKVVRDTKHLYKSKEIVFTLMGKINAVIK
                     AHRTDTQDLFGCGSDHDERYWMALIRQVLVAGLLHKDIETYGILKLSKEGAEFIKKPS
                     SFMMTEDHEYSEVEDDAIVAASKSTGTADEALMVILKDLRKKEAKKLGVPPFVVFQDP
                     SLEDMALKYPISIDELSNVHGVGEGKAKKYGKPFIELIARYVEENDIIRPDDLIVKST
                     GANSANKLYIIQNIDRKLSLEDIAKAKGLTMDNLLKEMEQIVYSGTKLNIKYWVDEIL
                     DDDQQEEIHDYFMDSQTDKIEDALKEFDGEYDIEELRLMRIKFISEVAN"
     gene            complement(2862..3098)
                     /locus_tag="P5335_08560"
     CDS             complement(2862..3098)
                     /locus_tag="P5335_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74968.1"
                     /translation="MSRRIKLIWDFRGPASSKTAEHHEIHLKEFITAEKLPINITGFE
                     IYNEMYAIAFMVVSDENMIAVRDALKPHRGELFE"
     gene            3234..4763
                     /locus_tag="P5335_08565"
     CDS             3234..4763
                     /locus_tag="P5335_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ74969.1"
                     /translation="MKNNYLTKIHVLFLFVINFGFSQTNYTVSAIPHQIYTSTATPIT
                     TSDDLHSGVIPIGFDFNYFGNNFNQLTISTNGYVSFNLNLASTFSEWNLNAQISQLNI
                     KNSIFACYHDMYNSNQTGSLTYSLVGYAPYRKFVVLFENQPHFSCTQLKSTFQLILYE
                     TFNFIDVQIVDKPVCSTWNGGNALIGIINESGSISYTPPGRNTGVWTASEEGWRFARP
                     NVSSVYYYTVCDNDLDGFESFNLDLIKEAIDSNLPQNVTIHETLSDAATGVNALPGTN
                     YININPYNQTLYAVGGGTITEVEISVVDCANDYDSDSVSTDLEDVNGDGNLANDDTDG
                     DGIPDFLDNDDDGDMVLTEFEYVFDSGRSSDDITNVDTDLDGIPNYLDNDDDGDGILT
                     IDEDYNGNNNPMDDDTNSNGIADFLEESVALSLTDESQLKRAISIYPNPANNVININN
                     LSTFSIDNFKIFTINGSLIKELKNSDSYQNIDVSQLQSGVYFISIEVNNQKLNYKFIK
                     N"
     gene            4858..6414
                     /locus_tag="P5335_08570"
     CDS             4858..6414
                     /locus_tag="P5335_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRZ74970.1"
                     /translation="MPKELLIQVAPEVAANDFLLKDHVAKMIRVTLSEVQHISIVKRS
                     IDARQKAIKINLKLIVFFQGEPFSEQKIELPHYPNVANQKEVIIVGAGPAGLFAALQL
                     IELGLKPIVIERGKDVRGRRRDLKAINVDHIVDEDSNYCFGEGGAGTYSDGKLYTRSK
                     KRGDVDRILQLLVGFGASPDILIEAHPHIGTNKLPQIIQDIREKIIECGGQVLFETRV
                     TDFIVKNNEIQGIVTQKGESISANKVILATGHSARDIFELLDRKKILIEAKPFALGVR
                     AEHPQELIDSIQYSCDFRGDYLPPAPYSIVKQVNGRGMYSFCMCPGGVIAPCATSPGE
                     VVTNGWSPSKRDQATANSGIVVELKLEDFKPFAKFGPLAGMEFQKSIEQKAWHLAGQT
                     QKVPAQRMIDFTQNKVSSSIPKTSYVPGTTSVEMGQVFPGFLTQIMREGFLQFGKSMK
                     GYLTNEAILHAPESRTSSPVRIPRDAQTLEHLQIKGLYPCGEGAGFAGGIVSAAIDGE
                     KCALKIKESL"
     gene            6513..7040
                     /gene="idi"
                     /locus_tag="P5335_08575"
     CDS             6513..7040
                     /gene="idi"
                     /locus_tag="P5335_08575"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HRZ74971.1"
                     /translation="MKEEKVILVNEKDEPIGLMNKLEAHEKAVLHRAFSVFILNDKNE
                     VMLQQRAHQKYHSPLLWTNTCCSHQREGETNIQAGTRRLSEEMGFSAELKELFHFIYK
                     APFDNGLTEHELDHVMIGYYNNKPIINPDEVEDWKWMSIEAIKEDMQIHPDIYTVWFK
                     IIFDEFYHYLEEHKL"
     gene            7071..7481
                     /locus_tag="P5335_08580"
     CDS             7071..7481
                     /locus_tag="P5335_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRZ74972.1"
                     /translation="MKVTVSRKAHFNAAHRLYRKDWTMEKNDLVFGKCNNPNFHGHNY
                     ELIVSVTGEIDTETGFVMDVKILSDLIKEHIEEAFDHKNLNVDVPDFFDLNPTAENIA
                     VVIWNKLRPQIKNELELEVVLYETPRNFVTYRGN"
     gene            7483..7938
                     /locus_tag="P5335_08585"
     CDS             7483..7938
                     /locus_tag="P5335_08585"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRZ74973.1"
                     /translation="MALKVGDKIPRFTAKDTNGNIFDSSIVLDKKPAVIYFYPKDDTP
                     GCTTQACSFRDQYEDFTDLGAEVIGVSSDSIASHQKFVSKYKLPFSLLSDEDKNIRKL
                     FGVPTALFGLLPGRVTYVTDENGIIRLIFDNLSAKIHIEKALQMLKKIV"
     gene            8000..>8211
                     /locus_tag="P5335_08590"
     CDS             8000..>8211
                     /locus_tag="P5335_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HRZ74974.1"
                     /translation="MTTPLYPFTFEPIFKERIWGGTKLRDYLNKPISSEITGESWELS
                     TVPNDVSVINNGILKGKNLNELIDQFP"
BASE COUNT         2837 a         1246 c         1519 g         2609 t
ORIGIN      
        1 cttatttcca ttaaacaaac ggctaaacaa tcacccatta ctaattgtgc tgttgtactg
       61 tttgttggtg ctaaattatt cggacaagct tctttttcaa cagttgtatc taaaacataa
      121 gttgaatttt tagctaaaaa ggaagatagg tttccggtca tggcaattaa agtgttacca
      181 aaacgattta aaatgggaac taaaattttt atttcaggac tattgccact ttttgaaatg
      241 caaataatga tatcgttggg ttgtaccatt cctaaatcac cgtgaactgc ttctgatgca
      301 tgtaaaaaca aggagggtgt tccggtagaa tttaaagtgg ctacaatttt ttgagcaata
      361 atggcacttt tcccaattcc ggttacaatc aatcgtcctt ttgattcata aatcaaacgt
      421 acgcaatcag ctaagtcatc tgtcagtaat tcagagagtt ttgaaatggc tttactttct
      481 gataaaatgg tttctttagc ggttgctaaa attttttctt ttgttatcaa aacggaatag
      541 atataaaatt tgtatgaata aaagaaagtt gtatctttat ggatgcaaat ttaattaaaa
      601 taccattaca taagaatgaa tcccaacgaa attgatattc acaaggaatt aaaaaagtat
      661 tttggtttcg atcactttaa aggcttacaa gagcaagtca taaaaagtat tttgaacaaa
      721 gaaaatactt ttgttataat gccaacaggt ggtggtaaat cgttgtgcta ccaactgcct
      781 gctttaattc aggacggaac tgcaattgtg gtttcgcctt tgattgcatt aatgaagaac
      841 caagttgatg ccatcagaag tgtttcttct gaagatggta ttgcacatgt attgaattct
      901 tcattaacga aaacagaaat aaatcaagtt aagaaagata ttgtttcagg aattaccaaa
      961 ttattatatg ttgctcccga atctttagca aaagaagagt atgtggattt tttaaaatcg
     1021 gtgcctattt cattcatagc cattgatgaa gctcattgta tttcagaatg gggacatgac
     1081 ttccgaccag aatatagaaa cctaaaaaat attattaaac atttgggaaa tgtaccggtc
     1141 attggattaa cagcgaccgc cactccaaaa gtgcaagaag atattcttaa aaatttagac
     1201 atgtcagatg ctacaaccta taaagcatcg ttcaatcgtc ctaatttatt ttatgaaata
     1261 cgcccaaaaa caaaaaatat agaatcggat attatcaaat ttataaaaca taataaaggt
     1321 aagtccggta ttatttattg tttaagcaga aaaaaggtag aagaaattgc ccaagtttta
     1381 caagttaacg gaatcagtgc cgttccttat catgcaggat tagatgctaa gaccagagcg
     1441 aagcatcaag atatgttttt gatggaagat gtagaagtag ttgtggcaac aattgctttt
     1501 ggtatgggaa ttgacaagcc ggatgttcga tttgtaattc accatgatat tcctaagtca
     1561 ctggaaagtt actatcagga aactggtcgt gcaggtcgtg atggaggcga aggacattgt
     1621 atggcttatt attcttataa agatgttgaa aaactagaaa aattcatgtc aggtaaacct
     1681 gttgctgagc aagaaatagg ttttgccttg ttacaagagg ttgttgctta tgccgaaact
     1741 tcgatgtcaa gacgtaaatt tttattacat tattttgggg aagaatttga taatgaaacc
     1801 ggggaaggtg gtgacatgga tgacaatgtt cgcaatccta aagcgaaagc agaagctaaa
     1861 gacgatgtag taaaagtgct taaggttgtt cgggatacaa aacatttata taaatcgaaa
     1921 gaaatcgttt ttactttaat gggtaaaata aatgccgtaa ttaaagcaca tagaactgat
     1981 acacaagacc ttttcggttg cggtagtgat catgatgaac ggtattggat ggctttgata
     2041 cgtcaagtat tagttgcagg attgttacat aaagatattg aaacatatgg aattttaaaa
     2101 ctttcaaaag aaggagcaga gtttattaaa aaaccaagtt cctttatgat gactgaagat
     2161 catgaatata gtgaagtaga agatgatgca atagtagctg catctaaatc taccggcact
     2221 gcagatgagg ccttaatggt tatattgaaa gatttacgta aaaaagaagc taaaaaattg
     2281 ggtgttcctc cttttgtagt ttttcaagat ccttccttag aagacatggc tttgaaatac
     2341 ccaatttcaa ttgatgaatt gagtaatgtg catggtgtgg gagaaggaaa agcgaaaaaa
     2401 tacggaaaac cttttatcga attaattgct cgctacgttg aagagaatga cattattcgt
     2461 ccggatgatt taattgtaaa atcaaccgga gctaattcgg ccaacaaatt atacattata
     2521 cagaatattg acagaaaatt atctttagaa gatatagcta aagctaaagg actgactatg
     2581 gataatttgt tgaaagaaat ggaacagata gtttattccg gaacgaaatt aaatattaag
     2641 tattgggttg atgaaatcct ggatgatgat caacaagaag aaattcatga ttattttatg
     2701 gactctcaaa ccgataaaat agaagatgct cttaaagaat ttgatggtga atatgatatt
     2761 gaagaattgc gtttaatgcg aattaaattt attagcgaag tagctaatta gtttttagtc
     2821 tcagttttca gttagtatta tcagtaaact gcgactgaaa actattcaaa caactcccct
     2881 cgatgaggtt ttaaagcatc tcttactgct atcatgtttt catcggaaac aaccataaat
     2941 gcgatggcat acatttcatt gtatatttca aaaccggtaa tatttatagg taatttttca
     3001 gcagtgataa attcctttaa gtgaatttca tggtgttccg cagttttgga agaagcaggg
     3061 cctctgaaat cccaaatcaa ttttatcctt cttgacatct tttttttagc aaaagtaaat
     3121 tcatttatat tactttaata attctttcaa ctattatttt tattactttt taaacattag
     3181 ttagaaaaac atgaaaaatt aaaacttttt tttacatttg aaaaaaaaat catatgaaaa
     3241 acaattactt aacaaaaatt cacgttcttt ttttatttgt tattaacttc ggtttttcac
     3301 aaacaaacta taccgtttct gcaattccac atcagattta tacttcaact gctacaccaa
     3361 ttactacaag cgatgatttg cactctggag taattccaat tggttttgat tttaattatt
     3421 tcggaaataa ttttaatcaa ttgactatca gtacaaatgg ttatgtcagt tttaatttaa
     3481 atttggcaag cacttttagt gagtggaatt taaatgctca aatttctcaa cttaacataa
     3541 agaactcaat ttttgcatgt tatcacgata tgtataattc aaatcaaaca ggttctttaa
     3601 cttactcgtt agttggttat gctccatata gaaaatttgt tgtcttgttt gaaaatcaac
     3661 cacatttttc ctgtactcag ttaaaaagca catttcaatt gatattatat gaaacattta
     3721 atttcatcga tgtacaaatt gttgacaaac cggtttgttc tacttggaat ggtggaaacg
     3781 ctttaattgg tataataaat gaaagtggta gtatttccta cactcctccc ggaagaaata
     3841 caggagtttg gacagcatca gaagaaggat ggcgttttgc aagaccaaat gtaagttcag
     3901 tatattatta cacggtttgt gataatgatt tagatggatt tgaatcattt aatttagact
     3961 taatcaaaga agctatcgat tctaatttac cgcagaatgt aacaattcat gaaacattat
     4021 cggatgcagc cacaggtgta aatgctttgc ctggaacaaa ttatattaac ataaatccat
     4081 acaatcaaac actttatgcc gttggtggcg ggacgataac agaagttgaa atttcagttg
     4141 tagattgtgc aaatgattat gattcagatt ctgtttctac cgatttagaa gatgtaaatg
     4201 gtgatggaaa tttagctaat gacgatactg atggtgatgg gattcctgat tttctcgata
     4261 atgatgatga tggagatatg gttttaaccg aatttgaata tgtttttgat tccggaagaa
     4321 gttcagatga tataactaat gttgatacag atttagacgg aattcccaat tatctggata
     4381 atgatgatga cggagatggc atattaacta ttgatgaaga ttataacgga aataataatc
     4441 caatggatga tgacacaaat tctaacggta ttgctgactt tttagaagaa agtgtagcat
     4501 tgagtttaac tgatgaatcg caattaaaaa gagcaatatc catttaccca aatccggcaa
     4561 ataatgtgat taacatcaat aatttaagca ctttttcaat tgataatttt aaaatattta
     4621 cgattaatgg tagcctaatc aaagaattga aaaattctga ttcctatcaa aatatagatg
     4681 tgagtcaatt acaatccgga gtatatttta tctccataga agtaaataat caaaaactaa
     4741 attataagtt tattaaaaat tagttttttt aaaatcttgt tctaaaaggt ttagcaattg
     4801 ctgaaccttt ttttatgcgg tacaaattaa aaaaattact tttgcaaaaa gataacgatg
     4861 cccaaggaac ttctcattca agtagcaccc gaagttgccg ccaatgattt tttattaaaa
     4921 gatcatgtgg cgaagatgat tcgtgttaca ctatcagaag ttcagcatat ttcgattgta
     4981 aaacgttcaa ttgatgctcg tcagaaagct attaaaatca atttgaagtt gatcgttttt
     5041 tttcaaggag aacctttttc tgaacaaaaa atcgaattac cccattatcc aaatgtcgcc
     5101 aatcaaaaag aagttatcat tgttggagca ggtccggcag gactttttgc cgctcttcaa
     5161 cttatagaat taggtttaaa acctattgtt attgaacgtg ggaaagatgt tcgcggtcgt
     5221 cgccgagatt taaaagccat caatgttgac catattgtgg atgaagattc caattattgt
     5281 tttggtgaag gcggagcagg aacttattcc gatggaaaac tctacacgcg gtccaagaaa
     5341 cgtggtgatg tcgatagaat tctacaatta ttagtcggtt tcggagcttc tccggatatt
     5401 ttaatagaag ctcatccaca cattggaacc aataaattac cacaaattat tcaagatatt
     5461 cgcgaaaaaa tcatcgaatg tggcggacaa gttttgttcg aaacccgtgt gacggatttt
     5521 attgtcaaaa acaatgaaat acaaggaatt gttacccaaa aaggagaatc aatttcggcc
     5581 aacaaagtca ttttagccac cggacattcc gctcgtgata tttttgaatt actagataga
     5641 aaaaaaattc ttatcgaagc caaacctttt gccttagggg ttcgtgcaga acatccacag
     5701 gaattgattg attcgattca atacagttgt gattttcgag gtgactattt accgccggct
     5761 ccctattcaa tcgttaagca agttaatggt agaggcatgt attccttttg tatgtgtccg
     5821 ggaggcgtaa ttgctccgtg tgctacaagt ccgggtgaag tggtgacgaa tggttggtcg
     5881 ccgtcaaaaa gagatcaagc aacagccaat tccggtattg tggtggaatt aaaattagaa
     5941 gattttaaac cctttgccaa attcggacct ttagccggaa tggaatttca aaaatccatc
     6001 gagcaaaaag catggcattt ggcaggacag acacagaaag ttcctgcaca acgcatgatt
     6061 gactttacac aaaataaagt atcttcttcc attcctaaaa cgtcttatgt tccgggaaca
     6121 acttcggttg aaatgggaca ggttttcccc ggatttttaa cccaaatcat gcgagaaggt
     6181 tttttacaat tcggtaaatc aatgaaagga tatttaacca atgaagccat tttgcatgca
     6241 ccggaaagtc gcacatcatc accggttcga attcccagag atgctcaaac cttagaacat
     6301 cttcaaatta aaggactcta tccttgtgga gaaggagcag gatttgccgg aggaattgtt
     6361 tctgctgcca tcgatggcga aaaatgtgcg ttgaagatta aggagagttt ataaatctta
     6421 tagacttcaa cacccgttaa acttttcaca aaaccatata aatcgaaata aaaacgaact
     6481 ttttcatacc tttgaagagc tataaaagtg ctatgaaaga agagaaagta attttagtca
     6541 atgagaaaga tgaaccaatt ggtttgatga ataaattgga agctcatgaa aaagcggttt
     6601 tgcaccgtgc cttttctgtt tttattttaa atgataaaaa cgaagtcatg cttcaacagc
     6661 gagctcatca aaaataccat tctcccttat tatggaccaa tacgtgttgt agtcatcaac
     6721 gggaaggtga aaccaacatt caagcaggga cgagacgatt gagtgaggaa atgggttttt
     6781 ctgccgaatt gaaggagttg tttcatttta tttataaagc tccttttgat aatggtttga
     6841 cagagcatga attagatcat gtgatgattg gatattacaa taataaacca ataattaatc
     6901 ctgatgaagt ggaagattgg aaatggatga gtattgaagc cattaaagaa gacatgcaaa
     6961 ttcacccgga catttatacc gtttggttta aaattatttt tgacgagttt tatcattatt
     7021 tagaagagca caaactataa ccttttacca attaccttta accatttgcc atgaaagtta
     7081 ctgtttccag aaaagctcat tttaacgccg ctcatcgatt gtatcgtaaa gattggacga
     7141 tggaaaaaaa cgatttggtt tttggtaagt gtaataatcc aaattttcac ggacataatt
     7201 atgaattaat tgtcagtgta accggagaaa ttgacacgga aacaggtttt gtcatggacg
     7261 taaagatttt atccgattta attaaagagc atattgaaga ggcttttgac cataaaaatc
     7321 taaatgtaga tgtacctgat ttttttgatt tgaatccaac agctgaaaat attgccgttg
     7381 taatttggaa taaattacga cctcaaataa aaaatgaatt agaattggaa gttgttttgt
     7441 atgaaacacc acgcaatttt gtaacctata gaggaaacta aaatggcact gaaagttgga
     7501 gacaaaattc ctcggtttac cgcaaaagat acaaacggaa atatttttga cagttcaata
     7561 gtattggata aaaaaccggc agtaatttat ttttatccaa aagacgatac accgggttgt
     7621 acaacgcaag cttgttcgtt tagagaccaa tatgaagatt ttaccgattt aggtgctgaa
     7681 gtaataggag tcagcagtga ttcaatcgct tctcatcaaa agtttgtttc gaagtacaag
     7741 cttcctttta gtttattatc ggatgaagat aaaaatatca gaaaactttt tggtgtacca
     7801 accgctttat ttggacttct tccaggacga gtgacttatg taacagatga aaatggaatt
     7861 atacgattaa ttttcgacaa tttatcggct aaaattcaca ttgaaaaagc tcttcagatg
     7921 cttaaaaaaa tagtataagt gtttgataag aagatagtgt caaatcaatt gcttattttt
     7981 gacctttgca agaaaaaaaa tgaccacacc actttatcct tttacttttg agcccatttt
     8041 taaagaacgc atttggggag gaacaaaact acgcgattat ttgaacaaac ccatttcttc
     8101 tgaaattaca ggagaaagct gggaattatc aacggttcca aatgatgtaa gtgttatcaa
     8161 taatggaatt ttaaaaggga aaaatctaaa tgaattaata gaccaatttc c
//
LOCUS       DAOTCW010000103         8012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_6300_length_8012_cov_41.970843, whole genome shotgun sequence.
ACCESSION   DAOTCW010000103 DAOTCW010000000
VERSION     DAOTCW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6300_length_8012_cov_41.970843"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="P5335_08595"
     CDS             complement(<1..713)
                     /locus_tag="P5335_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRZ74975.1"
                     /translation="MNYRIEKDTMGEVKVPADKYWGAQTERSRNNFKIGPSASMPKEI
                     IEGFAYLKKAAAFANHELGVLPIEKRDAIAQVCEEILAGKLDDQFPLVIWQTGSGTQS
                     NMNVNEVVANRAQVLAGFKIGEGEPVVKANDDVNKSQSSNDTFPTGMHIACYKAVMEK
                     TIPGVTQLRNTLKAKSEQYMNVVKIGRTHLMDATPLTLGQEFSGYVSQLDHGLRALNN
                     SLAHLSELALGGTAVGTGL"
     gene            900..1070
                     /locus_tag="P5335_08600"
     CDS             900..1070
                     /locus_tag="P5335_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74976.1"
                     /translation="MFDFQQYLGFILFLTVLTIGFWLMIFLVGFVHYWLGGAIMELIK
                     EKKAKRDQEQAS"
     gene            complement(1124..3604)
                     /locus_tag="P5335_08605"
     CDS             complement(1124..3604)
                     /locus_tag="P5335_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRZ74977.1"
                     /translation="MKKLISLFTLLFICSTSLLYSQERQVPNKIKVTGKVIDKSTSQP
                     LEYATIVLQNTKKPEMVTGGITNNKGEFDIEINAGMYDIRVEFISFKTVEFKQRNLQS
                     NTNLGTITLADDATQLDGVELRAERSTVDIKLDKRVYNVGQDMMVKGGTVSDVLDNVP
                     SVSVDVDGTVSLRGNENVRILIDGRPSNAININDALRMIPADAIDKVEVVTNPSARYD
                     AEGGGGIINIILKKGKNQGLNGTITGTIGDPTNRNLTGNINFKSDNFNLFTTQGFNYR
                     ESPGNSLTNTEYFNEDRTTRNYIYETRENSRNGNNYNGNFGFDWYLDKTITWTNTLSY
                     RKNSGDSPEHVEFDFYDANKVFQYTTIRELEQTNDSENIDLSSNFVKNFKKDGHKLTV
                     DASFSDNADLDFSFIDDENFEDVRTFNDQTSKRTILQTDYVLPIGKNGRFEMGYKGDF
                     NENLTDFTQEDFNESSNIWIKNDSISNIFEYKEKVNALYTQYGNKLNKFSYFFGLRWE
                     DSNIDVNQFITNDFTNKRYNNFFPSAVFSYELTESTNTSLSYSRRIQRPRGRFLNPFP
                     NYSSNINIFQGNPDLDPAMTHAIDLVYLQKWKKLTLSSSAYYNYTEDSFQFIRKESGA
                     FVETEVSGETVLTPIILTTPVNLATQYRFGFEFNLNYNPFKWWRLNGNFNFFRNETDG
                     EYIYTNYLGNQVVQNLDNIAISWTTRITSKITLPYKIDWQTNATYNAPQTTAQGKNLG
                     VVSANLAFSKDILKDKGTIALNVSDLFNSRKRISETNLPSSFAYSEMQWRERQINLSF
                     TYRFNKHKNDKERQQRREGGDEEFMGKP"
     gene            complement(3697..4041)
                     /gene="arsC"
                     /locus_tag="P5335_08610"
     CDS             complement(3697..4041)
                     /gene="arsC"
                     /locus_tag="P5335_08610"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634200.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HRZ74978.1"
                     /translation="MITIIHNPRCGKSREGLAILEQSGKPFQVIKYLDKNPTEAELKV
                     IIEKLQLAPIDLVRKKESVWIEQFKGKNLSDTEIIQAMVSNPILIERPIVMNDSRAII
                     GRPPHKIIDFIA"
     gene            4146..5081
                     /locus_tag="P5335_08615"
     CDS             4146..5081
                     /locus_tag="P5335_08615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRZ74979.1"
                     /translation="MYIEQINTKRFNFLFYLPIPVLFLGLMVVNYFSTQDVNVEDTLK
                     LFIDQFGVNLTFVILIGPLSAACLFLLFWVKFVHQQSIRSLTTSRKKVDWKRILFSFT
                     IWGLFSIVSTLILYYNAPENFILNFDASKFYIFLILAIVLIPLQTSFEEYLFRGYLMQ
                     GIGVVTKSKLAPLLITSILFGLMHIANPEVGKLGYIIMVYYIGTGFFLGIITLMDDGM
                     ELALGFHAANNLVGALLVTADWTAFQTHSIFKDVSEPSASYDVLFPVLVIFPILLFIF
                     GRKYEWTNWKEKLSGTVREYNVEKELINIKGKENE"
     gene            5074..6129
                     /locus_tag="P5335_08620"
     CDS             5074..6129
                     /locus_tag="P5335_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRZ74980.1"
                     /translation="MNSDVTYHNVHNKFKLNGFHLNREDLCTFAYSFIKEGEDFEKPV
                     GDFLLDWFDDRSYLEINTSGSTGTPKLIRVDKQAMVSSALATGDFFDLQPGDRVLHCL
                     PAKYVAGKMMFIRGFILGLEIDFVAPSSNPLERIDHDYDFAALVPMQAQYSLNKLHHI
                     KKIIIGGAKITKPLEEKLLKVKSEIYETYGMTETITHIAAKRIGEEAFTVFPNVMLSQ
                     DDNNCLLIKAPSISNGIIETNDIVEMVSENEFIWLGRYDNVVNSGGIKLIPEQIEEKL
                     ATRIPRRYFVIGQPDPVLGEKLVLIVEGEPITIEDSVFEVLDKYEKPKEIHFVKHFEE
                     TATGKIVRKETLSKFMY"
     gene            complement(6187..7812)
                     /locus_tag="P5335_08625"
     CDS             complement(6187..7812)
                     /locus_tag="P5335_08625"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRZ74981.1"
                     /translation="MSKIFLVILVFFGFQQINAQEFTRRDSLQGSLRPERTCFDVLRY
                     DLNLTIEPEYKTVKGYNDITFKVIEKTQKIQLDLFENMVVDSIIFNEKKLQYKREFDA
                     VFISFLSELPLNSEQKIRFYYNGNPLVAKRAPWDGGFVWKKDSNGNDFVGVAVQGTGA
                     SLWYPVKDHQTDEPDRGASVKVAVPNGLMNVSNGKFIGNENLNNGFTRWDWEVKNPIN
                     NYNIVINIGDYVNIHEKLDDLDLDYYVLAENEVVARKHFEEVMPMMECFQQKFGTYPF
                     VNDGYKLVETPYLGMEHQSAVAYGNKYTKGYLGNDMTGTGIGLEFDFIIIHETGHEWF
                     GNSITSKDIADMWIHESFTTYSECVYVECQFGYEKAMAYINGQKKLIANQKPVIGQFG
                     VNYKTGNSDMYYKGALMLNTIRHIINDDTKWWAMLKKYAETYRHKIIETKDVVAFFSK
                     ESGLNLSPIFDQYLKHKAFPELELKFNEGTIDFRWKTDVENFKMPFECKIGTKSLRYE
                     ATNAWQSAPIQLKNPTDFKLDENKFLFTSSVVK"
BASE COUNT         2318 a         1301 c         1511 g         2882 t
ORIGIN      
        1 tttaatccgg ttccaacagc agttccacct aatgctaatt cggataaatg ggctaatgaa
       61 ttgtttaacg ctcttaagcc gtggtctaat tgtgacacgt aaccggaaaa ttcctgtcct
      121 aaggtgaggg gagtggcatc cattaaatgg gtacgaccga ttttcacgac gttcatgtat
      181 tgttccgatt ttgcttttaa agtgttgcgg agttgtgtta cacccggaat tgttttttcc
      241 atgacggctt tgtagcaagc gatgtgcatt ccggttggga aagtatcgtt agaggattgt
      301 gatttattga cgtcgtcgtt tgcttttaca acgggttcgc cttcgccaat tttgaatccg
      361 gctaaaactt gagcacgatt agcgaccact tcgttgacgt tcatgttgct ttgcgtacca
      421 gaaccggttt gccaaatgac taatggaaat tggtcgtcta attttccggc caatatttct
      481 tcacacacct gagcaatggc gtcgcgtttt tcgattggaa gcacgcctaa ttcatggtta
      541 gcaaaagcgg ctgctttttt gaggtaagca aaaccttcta taatttcttt aggcatagag
      601 gcagaaggtc cgattttgaa gttgtttctg gaacgttctg tttgagctcc ccaatattta
      661 tcggcaggaa cttttacttc acccatggtg tctttttcga tacggtagtt cattgttgtg
      721 tttttattaa ttgtgaatga tatagtctgt cattggtttg tattgcagtg aaagctattg
      781 aaaaaatagt catgcaaatt tagttattaa cattactatt ttaaaatttt tttaggttta
      841 tgttgataaa atgataatgt tgatttatat ttgtcactga atcagaaaaa ttccgaaaaa
      901 tgtttgattt tcaacagtat ttaggcttca ttctcttcct taccgtttta accatcggtt
      961 tttggttgat gatatttttg gtaggttttg ttcactattg gttgggcgga gctattatgg
     1021 aattaatcaa agagaaaaaa gcaaaaagag atcaggaaca agcatcttaa tatattaaaa
     1081 tgttttgtaa caaaaaggga cgctattgcg tccctttttg tttttatggt ttgcccatga
     1141 attcttcgtc accaccttct cttcgctgtt gtctttcttt atcgttttta tgtttgttga
     1201 aacggtaggt gaaggaaagg ttgatttgtc tttctctcca ttgcatttca ctgtaggcaa
     1261 acgaactcgg aagattagtt tcggagattc tttttctgga attgaaaagg tcgctcacgt
     1321 ttaaggcaat ggttcctttg tcttttaata tatctttact gaaagcaagg tttgcactaa
     1381 caactcctaa attctttcct tgtgcagtgg tttgcggggc gttgtaggta gcattggttt
     1441 gccaatcaat tttgtagggc aaggttattt ttgaagtgat tctggttgtc cagcttatgg
     1501 caatattatc taagttttga acgacttggt ttcctaaata gtttgtataa atgtattctc
     1561 catcagtttc atttctgaaa aagttgaagt taccgtttaa tctccaccat ttgaatgggt
     1621 tgtaattcaa gttaaattca aatccgaaac gatattgggt ggctaagtta accggggttg
     1681 taaggatgat tggcgttaaa acagtttcac cgctcacttc ggtttctaca aaagcaccgg
     1741 attcttttcg gatgaattgg aatgaatctt cggtatagtt atagtaagct gaactactca
     1801 aggttaattt tttccatttt tgaagatata ccaggtcaat ggcatgggtc atggccgggt
     1861 ctagatcagg atttccctga aagatattga tgttactgga atagtttgga aaaggattca
     1921 ggaatctacc tcttggacgt tgaattcttc tgctgtaact taatgaagtg tttgtgcttt
     1981 cagttaattc ataggagaaa acggcacttg ggaaaaagtt attatagcgt ttattggtaa
     2041 aatcgtttgt gatgaattga ttcacgtcaa tgttagaatc ttcccaacgc aaaccgaaga
     2101 aataggagaa tttgtttagt ttgtttccgt attgtgtata gagtgcgttt actttttctt
     2161 tgtattcaaa gatgttgctg atgctgtcgt ttttaatcca aatgttagaa ctttcgttaa
     2221 aatcttcttg agtaaagtcg gtcaaatttt cgttaaaatc tcctttgtaa cccatttcaa
     2281 aacgtccatt tttaccaatt ggtaaaacgt aatcggtttg tagtatggtt cgttttgatg
     2341 tttggtcgtt aaaagttctt acgtcttcaa aattttcatc atcaatgaat gaaaagtcta
     2401 aatcggcatt gtctgaaaag gaagcatcaa cggttaattt gtgtccgtct tttttgaaat
     2461 ttttaacaaa attagaggac aagtctatgt tttcactatc gtttgtttgt tctaattcac
     2521 gaattgtagt gtattgaaat actttattgg catcgtaaaa atcaaattca acatgttcag
     2581 gactatctcc cgaattttta cggtaagata atgtatttgt ccaagtaatc gttttgtcta
     2641 aataccagtc aaagccaaag tttccattat aattgtttcc attacgggaa ttttctctgg
     2701 tttcatagat atagtttcga gtagttctgt cttcgttaaa gtattcggta tttgttaatg
     2761 aattcccggg actttcgcgg taattgaaac cttgtgtagt gaaaagattg aaattatcag
     2821 atttgaaatt gatgttacct gtaagatttc gattagttgg atcgccaatt gttccggtta
     2881 tagttccgtt taaaccttga tttttacctt ttttcaagat tatattaata ataccgccac
     2941 caccttccgc atcataacga gcggatggat ttgttacaac ttccacttta tcaatggcat
     3001 ctgccggaat cattcgtaag gcatcgttaa tattgattgc gtttgaaggt ctgccgtcaa
     3061 ttaatattcg aacattttca ttccctctta agctcacggt tccatcaaca tcaacagata
     3121 cggaaggaac gttgtctaga acatcgctca cggttccgcc ttttaccatc atatcctgac
     3181 ccacattgta cacgcgtttg tctagtttaa tatctacggt ggaacgttcg gcacgtaatt
     3241 caactccgtc taattgggtt gcatcatcgg ctaaagtgat agttcctaaa ttagtgtttg
     3301 actgtaagtt acgttgttta aattctacgg ttttgaaaga aataaattcc actctgatgt
     3361 catacatacc ggcattgatt tcgatgtcaa attccccttt gttgttggtg attccgcctg
     3421 ttaccatttc cggtttttta gtattctgta aaacaatggt ggcatattct aagggttgag
     3481 aagtagattt gtcaattact tttccggtaa cttttatttt gtttggtact tgtctttcct
     3541 gactgtaaag gagtgaagta ctgcaaataa aaaggagtgt aaataaggaa attaattttt
     3601 tcatgaatta aatattatgt gcctgaatta agactgcaaa attacttatt ggtttatccg
     3661 aaaaatccca aagatttgtt aaatgattgt tatgctttaa gcaataaaat caataatttt
     3721 atggggtggt ctgccaataa ttgctcggga atcattcatt acaattggtc tttctattaa
     3781 aataggattt gaaaccatgg cctgaatgat ttcagtatct gacaggtttt tacctttaaa
     3841 ttgttctatc caaacggatt cttttttcct gactaaatca ataggagcca attgaagttt
     3901 ttcaataatg acttttaatt ctgcctcagt agggttttta tcaaggtatt taatgacttg
     3961 aaaaggtttt cctgattgtt ccaggatggc taagccttct ctggatttgc cacatcttgg
     4021 attgtgaata atggtaatca ttcttatttt tttgcaaagt aacagcatca aaattcaaat
     4081 tttgttaaaa aaaatgtaac atgtgcattt acaaaagtcc tatatttgaa aacaaatgct
     4141 ttcctatgta tattgaacaa atcaacacca aaaggtttaa ttttttattt tatttgccca
     4201 ttcctgtttt gtttttagga ttaatggtgg tgaattattt ttcaactcaa gacgtgaatg
     4261 tggaagacac tttaaagctg tttattgatc agtttggtgt gaatttgact tttgtgattt
     4321 tgattggccc gttatcagcg gcttgtcttt tcttgctttt ttgggtaaaa tttgttcatc
     4381 agcaaagtat tcgaagttta acgacttccc gaaaaaaagt ggattggaaa agaattttgt
     4441 tttcttttac tatttgggga ttattcagta ttgtttccac cttaatccta tattacaatg
     4501 caccggaaaa ttttatactt aattttgatg cttcaaaatt ttatattttt ttaattctag
     4561 ccattgtttt aataccctta cagacgagtt ttgaagaata tttgttcagg ggttatttga
     4621 tgcaaggaat tggtgtggtg actaaaagta aattagctcc tttactgatt acgtctatct
     4681 tgtttggatt aatgcatatt gcaaatccgg aagtaggaaa gttgggttac atcatcatgg
     4741 tttattatat tggaaccgga ttttttttag gaattataac cttgatggat gatggaatgg
     4801 aattggcctt gggttttcat gctgccaata atcttgtagg agccctttta gtaacggcag
     4861 attggactgc ttttcaaacg cattccattt ttaaagatgt atcagagcct tctgcaagtt
     4921 atgatgtatt atttccggta ctggttattt tcccaattct tttgtttatt tttgggagaa
     4981 aatacgagtg gaccaattgg aaagaaaaat tatcaggaac tgttcgagaa tataatgttg
     5041 aaaaagaatt aattaatata aaaggaaaag aaaatgaata gtgatgttac ttaccacaat
     5101 gtacataaca agtttaaact gaatggtttt catttaaaca gagaagattt atgcactttt
     5161 gcttatagtt ttataaaaga aggcgaagat tttgaaaaac cggttggcga ctttttgtta
     5221 gattggtttg atgacagaag ttatcttgaa ataaacacct ccggttctac gggcacaccc
     5281 aaattgattc gtgttgataa gcaagcgatg gttagttcag ctctagcgac aggagatttc
     5341 tttgatttac aaccgggtga cagagtactt cattgtttac ctgctaagta cgtagccgga
     5401 aaaatgatgt ttattcgcgg ttttattttg gggttggaaa ttgattttgt tgctcctagt
     5461 tcaaatccgt tagagcgaat agaccatgac tacgattttg ccgcgttggt accaatgcaa
     5521 gctcaatatt cgttgaataa attgcatcac ataaaaaaaa taatcatagg tggtgccaaa
     5581 atcacgaaac cattagaaga aaaattactg aaagtcaaat cagaaattta tgaaacctat
     5641 ggtatgactg aaaccatcac gcacattgca gcgaagcgaa taggagaaga agcgtttacg
     5701 gtttttccaa atgtaatgct ttcgcaggat gataataatt gtcttttaat taaagcacct
     5761 tctatttcaa atggaatcat agaaaccaac gatattgtag aaatggtttc tgaaaatgaa
     5821 tttatttggt taggacgtta tgataatgtg gtaaacagtg gtggtatcaa attgattccg
     5881 gaacaaatag aagaaaaatt agccacccga atcccaagac gctattttgt gatagggcag
     5941 cctgatccgg ttttaggaga aaaattagtt ttgattgtgg agggagagcc gataaccatt
     6001 gaagattctg tttttgaagt gttagataaa tatgaaaaac ctaaagaaat tcactttgta
     6061 aaacattttg aagaaactgc aacaggtaaa attgttcgaa aagaaacgtt gagtaaattt
     6121 atgtattaat aaaattgcca ctgatttcct gaaaagagta tttcaattca aggaatcagt
     6181 ggtttattat tttacaacgc tactcgtaaa caaaaatttg ttttcgtcca acttgaaatc
     6241 agttggattc tttagctgaa ttggtgccga ttgccaagca tttgtggctt cataacgcag
     6301 tgattttgtg ccgattttac attcaaaagg cattttaaaa ttttctacat cagttttcca
     6361 tcggaaatcg atagttccct cgttaaactt caattctaat tcaggaaatg ctttgtgttt
     6421 taaatattga tcaaaaatgg gggatagatt taaccccgac tctttagaga agaaagccac
     6481 aacatctttt gtttcaataa ttttatggcg gtaagtttca gcatattttt tcagcattgc
     6541 ccaccatttt gtgtcatcgt tgatgatatg tcgaatcgta ttgagcatca aagctccttt
     6601 gtaatacatg tcggaattgc cggttttata atttacgccg aattgcccaa tgaccggttt
     6661 ttgatttgca attaattttt tctgaccatt gatataggcc atcgcttttt catagccaaa
     6721 ttggcattca acgtatacac attctgaata agtggtaaaa ctttcgtgaa tccacatatc
     6781 tgcaatgtct tttgaagtga tgctgtttcc aaaccattca tgaccggttt cgtgaataat
     6841 gatgaaatca aattctagcc cgattccggt gccggtcata tcatttccta gataaccttt
     6901 cgtatactta tttccataag ctacagcact ttgatgctcc atacctaaat aaggagtttc
     6961 aactaatttg tagccatcat ttacaaaagg ataggttccg aatttttgtt ggaagcattc
     7021 catcatgggc ataacttctt caaaatgttt acgggccact acttcatttt cagctaagac
     7081 ataataatct aagtctaaat cgtctaattt ttcatggatg ttgacataat caccaatgtt
     7141 aatgacgatg ttataattat tgattggatt ttttacttcc caatcccaac gggtaaagcc
     7201 attattgagg ttttcattgc ctataaattt accgttagaa acgttcatta aaccatttgg
     7261 aactgcaact ttgacggaag cacctctatc aggttcgtcg gtttgatggt cttttacggg
     7321 ataccacaaa ctggctccgg ttccttgtac tgccactccg acaaaatcgt ttccgttgct
     7381 gtcttttttc catacaaaac caccatccca tggtgcccgt ttagcaacta acggattgcc
     7441 attgtaataa aacctaattt tttgctcaga atttaaaggt aattcactaa gaaaactgat
     7501 aaaaacagca tcaaattctc ttttgtattg cagttttttt tcattgaaaa tgatactatc
     7561 caccaccatg ttttcaaata aatccaactg gattttctgc gttttttcaa ttactttaaa
     7621 ggtaatgtca ttgtaacctt tgactgtttt atattccggt tcaatggtca aattcaagtc
     7681 ataccgaagt acatcgaaac aagttcgttc cgggcgtaga ctaccttgta gtgaatcgcg
     7741 acgggtgaat tcctgagcgt tgatttgttg aaatccaaaa aacactagta ttacgagaaa
     7801 aatttttgac atggtttact tttcaattag ccgcaaattt gctgattaat aattttttta
     7861 aatcgattac taaaatagca ttaaaaatca attagtactt tacgtattaa ctctttttta
     7921 tgaatagagg gagactaact caattttttt tcaaatcgag ttttatgttc tcttgttaat
     7981 tagtgatttt gccttgttca aggagttttt tt
//
LOCUS       DAOTCW010000104         7942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_6385_length_7942_cov_31.793331, whole genome shotgun sequence.
ACCESSION   DAOTCW010000104 DAOTCW010000000
VERSION     DAOTCW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6385_length_7942_cov_31.793331"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1149)
                     /locus_tag="P5335_08630"
     CDS             complement(211..1149)
                     /locus_tag="P5335_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRZ74982.1"
                     /translation="MNKITQLFNIQYPIIQGGMIWNSGYKLASAVSNAGGLGLIGAGS
                     MYPEVLREHIQKCKKATSKPFGVNVPMLYPNIEEIMAIIVEEGVKIVFTSAGNPKTWT
                     SFLKEKGITVVHVVSSSKFALKAQEAGVDAVVAEGFEAGGHNGREETTTLTLIPMVKE
                     QIQIPLIAAGGIATGRGMLAVMILGADGVQMGSRFAASTESSAHSDFKQTILETKEGD
                     TQVTLKELAPVRLIKNKFYQDVQELYTKCPSTDELKQLLGRARAKRGMFEGDLIEGEL
                     EIGQIAGLIHEINPVKEIIETIITEFEIAKKEVMQL"
     gene            complement(1173..2783)
                     /locus_tag="P5335_08635"
     CDS             complement(1173..2783)
                     /locus_tag="P5335_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRZ74983.1"
                     /translation="MKKIYFLLLLFCSFSAISQEDAWVYFTNKPDADFFLQNPLDMLS
                     QRALDRRTTQNIALDLLDVPIAESYKSQIAAQPGITIMAQSKWMNCLHIRGTQAAIEA
                     LTSLSFVSSIQFANPTLNQSGRMASTNKEATATKNLDIQVDFSYGGSLNQVQMLGGDL
                     LHQQNFTGNGKIIAVMDNGFPGVNTTQPFQRLRDNNLILGGYDFVNRDEDVYEGGTHG
                     TMVLSTMGGFKETELVGTAPDAAYYLFKTEANTYENPLEESLWVEAAELADSLGVDVI
                     NTSLGYSTFDNPAYNYSYADMNGITTFIARGADLAFSRGMICVTSAGNSGNSSWQYIS
                     TPADAINTLAVGAVNASGVYASFSSIGPSSDGRVKPDVDAQGVSSTVATSNGSIANAN
                     GTSFSGPITAGMVACLWQALPTKTNAELIQLVKESAHLYQNPTAQLGYGIPNFNAALI
                     NALAVNDFETTPFMVYPNPFQDEISIVLDNKFEKAQLELFSVLGQKIKTFSVSGHQSI
                     DVTEVASGIYFYQITAGNNTFSGKLIKK"
     gene            complement(2810..3997)
                     /gene="mnmA"
                     /locus_tag="P5335_08640"
     CDS             complement(2810..3997)
                     /gene="mnmA"
                     /locus_tag="P5335_08640"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRZ74984.1"
                     /translation="MKRVIVGLSGGVDSSVAAYLLKEQGYEVIGLFMKNWHDDSVTIS
                     NECPWLEDSNDALLVAEKLGIPFQTVDLSEEYKERIVDYMFKEYENGRTPNPDVLCNR
                     EIKFDVFMKIALSLGADFVATGHYCRKAIIEKDGKEIYQLLAGVDDNKDQSYFLCQLS
                     QEQLAKSLFPIGELTKPQVREIAAQLDLITAEKKDSQGLCFIGKVRLPDFLQQQLKPK
                     EGNIIEIDGNDPIYKLEQPTFADAEDQLEFEAEPLHYLPTMGKVVGKHQGAHYFTIGQ
                     RKGLNVGGTKEGLFIIATDVESNTIYTGQAHNHPGLFRKALKIEPNEIHWVREDLRLK
                     NGVKMEVLARIRYRQALQKATIYQFDSGLYMEFEEPQSAITAGQFAAWYIKDELVGSG
                     VIS"
     gene            complement(4044..4781)
                     /locus_tag="P5335_08645"
     CDS             complement(4044..4781)
                     /locus_tag="P5335_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74985.1"
                     /translation="MMLRYSRSIFLLIMVISSFSIAYSQNNQLDEKGNKHGPWKGVYE
                     DTKYTKYEGRFEHGKEVGVFNFYDNTKVKKIIATRDFTANDGSCYTIFYNGKFKVSEG
                     KLVNKVYEGEWKFYHLKSDTIMNVENYKKGKLHGTKKVFYNTGLLAEESNYKEGLKEG
                     PYKKVAENGVVLEESNYKNNEYEGFAIFREAAGDKYSTGQYKNGKSVGKWKFYEKGKL
                     VKTENRSEHKRARPKSKNPQTKEKLKN"
     gene            5127..6062
                     /locus_tag="P5335_08650"
     CDS             5127..6062
                     /locus_tag="P5335_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRZ74986.1"
                     /translation="MKKFQKFAVIAFLAIGMVACSDDDSTPAPQTITDIAVANPDFST
                     LVTALERTGLDAVLDGEGEFTVFAPTNAAFTTFLSGANVNDVPVPVLTQILLNHVVSG
                     KVLSTQLTTGYIQTLATYNGVANGSNLEMYVSTANGVRLNGVSSVSSADISASNGVIH
                     VVDAVIGLPTIVTFATADPNFSDLVGALTSAGQPDFVSILSGAGPFTVFAPSNAAFDA
                     LDAEIPGVVGSLTTEQLTSVLTYHVASGNVLSSSLTNGQEITTLESGLLEVSLAGGAK
                     LIDEAGRTTNIVAVDVQAANGVIHVLGNVLIPSFD"
     gene            complement(6523..>7942)
                     /gene="yidC"
                     /locus_tag="P5335_08655"
     CDS             complement(6523..>7942)
                     /gene="yidC"
                     /locus_tag="P5335_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRZ74987.1"
                     /translation="TKDLFFEPVLTKEGTNQVLTLRLKAGNAQYLEYRYVLKPNEYML
                     DFAIRSQGLNTVLNTSNPLNLDWSIKTYRNEKSVSYENRYTELIFEYEDGKDDYLGQG
                     ESKSDDPVDVTYIAFKQHFFTSVLMTDTAFEKAVVVSENLVKDEEKDTLFTKQFKATV
                     PLAFKNGEVNYNMNWYYGPADYHILNAYDKNLDEIVPLGWGMFGWINRYIFIPTFDFL
                     IGFLPFGIAIIVLTIIVRIVMSPFTYKSYLSQAKMKVLRPEIQELTTKFAKDPMKKQQ
                     ETMKLYSKAGVNPMAGCLPAVMQIPVFYALFSFFPSAIDLRQKSFLWATDLSSFDSVI
                     NLPFYIPFYGSHVSLFPILASIAIFFYMKMTTGDQAMSAPPQEGMPDMGKIMKIMIYI
                     SPLMMLFFFNNFASGLSLYYFVSNAITIGIMLVIKNYIIDENKIHAQIQENKTKPKTM
                     NRFQRKMQEMMEEAEKQKNLKK"
BASE COUNT         2460 a         1442 c         1333 g         2707 t
ORIGIN      
        1 cctacttctt catttttttg agaaaataaa aaagtaatat tactaaaaaa aagcagtaaa
       61 aagagaagtt ttttatgcat tccaattaat gttatacaca aaaataattt attttgtttt
      121 ataaaacagt taatgaaaga ggtaatcatc aggaaaacca aaaaacgaac aaatcatgtg
      181 aataatgttt aattcatcac tctttaaaaa tcataactgc attacttctt ttttagcaat
      241 ttcaaattca gtaataatgg tctcgatgat ttcttttacg ggattgattt cgtgaatcag
      301 tccggcaatt tgaccgattt ccaattcccc ttcaattaga tcaccttcaa acataccgcg
      361 tttagccctt gctctaccta atagttgttt gagttcgtct gttgaaggac atttggtata
      421 taattcctgt acgtcttgat agaatttgtt tttgattaaa cgcacgggag ctaattcttt
      481 taaagttact tgggtatcgc cttctttcgt ttcgagaata gtttgtttaa aatcactatg
      541 ggccgaactt tctgtggaag cagcaaaacg acttcccatt tgaacaccgt ctgcaccaag
      601 aatcataacg gcaagcatac cacgaccggt ggcaattccg ccggcggcaa taagtggaat
      661 ttgaatttgt tctttcacca tgggaatcaa ggtcaacgta gtggtttctt cccgaccatt
      721 gtgccctccg gcttcaaagc cttccgcaac tacagcatcc accccagcct cttgggcttt
      781 caaggcaaac ttagaactac tgaccacatg aacaacagtg attccttttt cttttaaaaa
      841 agaagtccac gtttttgggt tacctgctga agtgaagact atttttacac cttcttcgac
      901 aataatcgcc atgatttcct cgatgttggg atacaacata ggtacattta ctccaaaagg
      961 ttttgaggtt gcctttttac atttttggat gtgttcccgc agtacttccg gatacatgga
     1021 acccgctccg atgagtccga gtccaccggc attactaaca gcacttgcta atttgtatcc
     1081 gctattccaa atcattccgc cttgaatgat tgggtattgt atgttgaaaa gttgagtaat
     1141 tttgttcata ttaactctga gccggtctcg ttttactttt tgattagttt accgctgaag
     1201 gtattgtttc ctgctgtaat ttgataaaaa taaattccgg aggccacctc agtcacatca
     1261 attgattgat gacctgaaac cgaaaatgtt tttatttttt gacccaaaac tgaaaataat
     1321 tccaactgag ccttttcaaa cttgttgtct aaaacaattg aaatttcatc ttgaaacgga
     1381 tttggataga ccataaaagg agtagtttca aaatcgttta cggctaaagc attgattaaa
     1441 gccgcattga aattcggaat tccataccct aattgagcgg ttggattttg gtaaagatga
     1501 gctgattctt tgaccaattg aataagttct gcattggttt tagttggtaa tgcttgccat
     1561 aaacaggcta ccattccggc tgtaattggt ccggagaaag aagttccgtt tgcatttgct
     1621 atagatccat ttgacgttgc aacagtagat gaaactcctt gagcgtcaac atcgggtttt
     1681 acacgtccat cagaagaagg accaattgag ctaaacgaag cataaactcc tgaggcattc
     1741 acggcaccaa ccgctaaagt attaattgca tctgcaggtg tagaaatata ttgccatgaa
     1801 ctgttgccgg aatttccggc agaagtgacg caaatcattc ctcgtgaaaa agctaaatca
     1861 gcaccccgag ctatgaaggt ggtaattcca ttcatgtcgg catacgaata attataagcc
     1921 ggattatcaa aagtagaata gcctaaagat gtattgatga catcaacacc taagctgtct
     1981 gctaattctg cagcttccac ccaaagagat tcttccaaag ggttttcata ggtgttggct
     2041 tcggttttaa ataaatagta ggccgcatca ggagcagtac ctaccaactc agtctctttg
     2101 aatcctccca tggtggaaag caccatggtt ccgtgggttc caccttcgta aacatcttca
     2161 tctcggttaa caaaatcata accacctaaa atgaggttat tatctcgtaa acgttgaaaa
     2221 ggttgtgtag tattgacacc aggaaaacca ttatccatta cagctattat tttcccgtta
     2281 ccggtaaaat tttgttggtg aagtaaatcg cctcccaaca tttgaacttg attgagtgaa
     2341 cctccgtaag aaaaatcaac ctgaatgtca aggtttttag tcgcagttgc ctctttattg
     2401 gtgcttgcca tgcgtccact ttgattcaag gttggattag caaactgaat tgaacttaca
     2461 aacgaaagag aagttaacgc ttcaattgct gcttgagtac ctcttatgtg caaacaattc
     2521 atccacttag attgggccat aatcgtgatg ccgggttgag cagcaatttg tgatttatag
     2581 gattccgcaa tgggaacatc caacaaatcc aatgcaatgt tttgagtagt tcttctgtct
     2641 aaggctcgtt gtgaaagcat atccaagggg ttttgcaaaa agaaatcggc atccggttta
     2701 ttggtaaaat aaacccaagc atcttcttgc gaaatcgcgg aaaaagagca aaacaataat
     2761 agtaaaaagt agattttttt catagcttgc tgattataag cgtaccaatt tacgaaatta
     2821 ctccggaacc aactaattca tcttttatat accaagcggc aaattgtccg gcagtgatgg
     2881 ccgattgagg ttcttcaaat tccatataaa gtccactgtc aaattgataa atagttgctt
     2941 tttgaagagc ttgacggtac ctgattcgtg ctaaaacttc catttttacg ccgtttttta
     3001 atcgtaaatc ctcgcgaacc caatggattt catttggttc aatttttaat gcttttcgga
     3061 ataaaccggg atggttgtgt gcttgtccgg tatagattgt atttgattct acatcggtag
     3121 cgatgataaa taatccttct ttggttcctc ccacgtttaa gcctttccgt tggccaatag
     3181 taaaataatg agctccttgg tgttttccaa ctactttacc cattgtaggc aaataatgta
     3241 atggctctgc ttcaaattca agttggtctt ctgcatcagc gaaggtgggt tgttcaagct
     3301 tatatatggg gtcatttcca tctatttcga tgatattacc ttctttaggt ttcaattgtt
     3361 gttgcaggaa atcaggtaaa cgaacttttc cgatgaaaca taacccttgc gaatcttttt
     3421 tctctgcggt aattaaatca agctgagctg caatttcacg aacctgtggt tttgtcagtt
     3481 cgccaattgg aaataatgat ttagccaatt gttcttgaga caattgacat aaaaagtagg
     3541 attgatcttt gttatcgtct actccggcaa gcaattgata gatttctttt ccgtcttttt
     3601 cgattatagc tttacggcag taatgacctg ttgcaacaaa atcagcccct aaagagaggg
     3661 cgattttcat gaacacatca aatttaattt ctcgattaca tagcacatcg ggatttggtg
     3721 tacgaccatt ttcatattct ttgaacatat agtccacgat acgttcttta tattcttcgc
     3781 ttaaatcaac agtttgaaaa ggaatgccga gtttttcggc aaccaaaagg gcatcgtttg
     3841 aatcttccaa ccaaggacat tcattagaaa tggtcacgga atcatcatgc caatttttca
     3901 taaacaaacc gatgacttca tagccttgtt ctttcaacaa ataggctgct acactggaat
     3961 ctactccgcc ggataaacca actataactc ttttcatacg cattaaaata gaaggcaaag
     4021 gtattattta cacttatatt tttttaattc tttaactttt ctttggtttg aggattttta
     4081 ctttttggtc tagctcgttt atgctcgctt cggttttctg tttttactaa ttttcctttt
     4141 tcataaaatt tccactttcc aactgatttg ccgtttttat attgacctgt tgaatactta
     4201 tcaccggcag cttctctaaa aattgcgaaa ccctcatatt cattgttttt ataatttgat
     4261 tcttctaaca ccaccccatt ctcggctacc tttttataag gtccttcttt taaaccttct
     4321 ttgtaatttg attcttcagc taaaagacct gtgttgtaaa atactttttt agtaccgtgc
     4381 agttttcctt ttttatagtt ttctacattc ataattgtat ccgattttaa atggtaaaat
     4441 ttccactccc cttcgtatac tttattgact aactttcctt cgcttacttt aaattttcca
     4501 ttgtaaaaaa tggtgtaaca agacccatca tttgcagtaa aatcgcgagt tgctataatt
     4561 tttttaactt ttgtattgtc atagaaatta aaaacaccta cttcttttcc atgttcaaac
     4621 cggccttcgt attttgtgta ctttgtgtct tcatatactc ctttccaagg gccgtgtttg
     4681 tttccttttt catccaattg attgttttga gaataagcaa ttgaaaaact acttattacc
     4741 attattaata aaaaaatact tctagaataa cgtaacatca taatgagtca atttttaaaa
     4801 aatttatgta aagcaaatat acagtgttag taatagtatc aaatgtaaac gggaaagatt
     4861 taattaaatt atttagaagt aagattacac ctttgaaaaa acaatttgat taatttaaaa
     4921 ttacaattgt taattttgtt tagttaaact ttagttaaag ttaaacaaaa ataatttagt
     4981 taaacatctt taaaaccctt atataataag aaaatttagt aaaataacaa tcgtttttat
     5041 atttaattgt ttaatcttta tattttaaac ataaacaaaa tataaaataa tttatatatt
     5101 tgcaccgaac aattaaatca ataaatatga aaaaatttca aaaatttgcg gtgattgcct
     5161 ttttggcaat tggaatggta gcttgtagcg atgacgattc gactccggca cctcaaacta
     5221 ttacggatat tgcagttgca aatcctgatt tcagtacttt agtaacagct ttagaaagaa
     5281 ctggtcttga tgctgtttta gacggtgaag gagaatttac agtttttgca ccaactaatg
     5341 ctgcctttac aactttttta agcggtgcaa atgtgaatga tgttccggtt ccggttttga
     5401 ctcaaatttt gttaaaccat gttgtgagtg gtaaagtttt atctacacag ttaactaccg
     5461 ggtacattca aacgttagct acatacaatg gagtagcaaa tggaagcaat ttagaaatgt
     5521 acgtgagtac agctaatgga gttcgcttaa atggagtatc atcagtttct tcagcagaca
     5581 tttcagcttc aaacggagtt attcatgttg ttgatgctgt aattggattg ccaactattg
     5641 taacttttgc tactgcagat ccaaatttca gtgacttagt aggagcttta acatcagctg
     5701 gtcaacctga ttttgtgtca attttatccg gggcagggcc atttacagta tttgcaccat
     5761 caaatgctgc atttgatgcc ttagatgctg aaattcctgg tgttgttgga tcattgacta
     5821 ctgaacaatt aacttctgta ttaacttatc atgttgccag tgggaatgtt ttatcttcat
     5881 cattgactaa cggtcaagaa atcacaactt tagaatcagg tcttttagaa gtgagtttgg
     5941 ccggtggtgc taagttaatt gacgaagcag gaagaactac aaatattgtt gcagtggatg
     6001 tgcaagcagc gaatggcgtt attcatgttt taggtaatgt actaatacct tcttttgact
     6061 aaggaaaatt tgctatgaaa aagaaaagct ccgattaatt tcggagcttt tttgtatttt
     6121 actttttcaa actgtttttt ttatacaaca tttgccattt cctacatttt aatttgaaaa
     6181 cgattcatag ttttctgttt aattttattc tcttgaatca gagcatagat tttcatttca
     6241 gtaatactat agtttttaac cactaacatg aattgaaaac attttatagt tagtcaaaaa
     6301 ttatgatgtt actttaattg aacgatttgt aattatgtta taaatccaat ttgaaaagga
     6361 atgtaggcag agctcaaaat ctaaaaaaca aaaaatactc cggtaagaac aaaacaattt
     6421 actttaaata taagataaga aaaatcaaat atacctaata aggtactgca aattactatg
     6481 aaaaagaaaa gctccgaaat tattcggagc tttttttgtg ctttactttt tcaaattctt
     6541 ttgtttttct gcttcttcca tcatttcttg catttttcgt tggaagcggt tcatggtttt
     6601 cggtttcgtt ttattttctt gaatttgagc atgaatttta ttctcatcga tgatgtaatt
     6661 tttaattact aacataattc caatagtaat ggcattcgat acaaaataat acaaacttaa
     6721 accggaagcg aagttattaa agaagaacaa catcattaga ggtgaaatat aaatcatgat
     6781 tttcatgatt ttacccatat ccggcatacc ttcttgtggt ggagcactca tcgcctgatc
     6841 tcctgttgtc attttcatat aaaagaaaat agcaatagaa gccaaaattg gaaataaact
     6901 cacatggctt ccataaaaag gaatgtaaaa cggtaaatta ataaccgaat cgaacgagga
     6961 taaatctgtt gcccaaagga aacttttttg tcttaaatct atcgccgaag gaaagaaact
     7021 aaacaaagca taaaacaccg gaatttgcat aacagctggc aaacaacctg ccatcggatt
     7081 aacacctgct ttagaataca atttcattgt ttcctgttgc tttttcattg ggtctttcgc
     7141 aaattttgtc gtcaattctt gaatttccgg acgtagtact ttcatttttg cttgtgacaa
     7201 ataggattta tacgtaaaag gcgacatgac tatacgaaca ataattgtta atacaataat
     7261 ggcaattcca aaaggtaaaa atccaatcag aaaatcaaac gtaggtataa aaatataacg
     7321 gtttatccat ccgaacattc cccatcctaa aggaacaatc tcatctaaat ttttatcgta
     7381 tgcatttaaa atatggtaat ccgcaggacc ataataccaa ttcatattat agtttacttc
     7441 tccattttta aaggctaatg gaacggtagc tttgaattgt ttggtgaata aggtatcttt
     7501 ttcttcatct tttactaaat tttcagaaac aaccactgct ttttcaaaag ctgtgtcggt
     7561 catcaaaaca gaagtaaaaa aatgttgttt aaaagcaata taggtaacat caaccggatc
     7621 atctgatttt gattctcctt gacctaaata atcatcttta ccatcttcat attcaaaaat
     7681 aagttcagtg tagcgatttt catacgatac acttttctca tttcggtaag ttttgatact
     7741 ccaatctaaa ttcaagggat tagacgtatt taaaacagta ttcaaacctt gtgaacgaat
     7801 tgcaaaatca agcatatatt cgtttggttt caacacgtaa cgatactcca aatactgtgc
     7861 attaccggct ttcaatcgaa gtgttaatac ttgattagtt ccttctttgg ttaaaaccgg
     7921 ttcaaaaaat aagtctttcg ta
//
LOCUS       DAOTCW010000105         7937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_6392_length_7937_cov_40.272647, whole genome shotgun sequence.
ACCESSION   DAOTCW010000105 DAOTCW010000000
VERSION     DAOTCW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6392_length_7937_cov_40.272647"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /gene="cmk"
                     /locus_tag="P5335_08660"
     CDS             complement(<1..668)
                     /gene="cmk"
                     /locus_tag="P5335_08660"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRZ74988.1"
                     /translation="MNNITIAIDGFSSTGKSTLAKQLAKHLGYVYVDSGAMYRAVTYF
                     AMQNKLVSEDYLDKEGLIKKLPEIKLHFQFNPELGFAEMFLNDVNIESEIRTIEVSRL
                     VSKIAEISEVRAKLVEQQQAMGNNKAVVMDGRDIGTVVFPDAELKLFMTASAHTRAKR
                     RHDELIEKGQHVSYEDVLQNVQERDYIDTHRDDSPLVKAHDAIEIDNSTLTKKEQFDL
                     VLDL"
     gene            complement(727..1746)
                     /gene="porQ"
                     /locus_tag="P5335_08665"
     CDS             complement(727..1746)
                     /gene="porQ"
                     /locus_tag="P5335_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HRZ74989.1"
                     /translation="MFKNFLTLLFVLFCSVSYSQIGGQSVYQFLNLVTSPRQAALGGK
                     VITNFDYDVNAAHFNPASINQEMHNHLSVNYASYYGEVTYGTASYAYTYDRHVQTFHA
                     GVNYVNYGNFEGRDESGALTSDFTGSEVALSFGYSYNIPFSDFYIGANAKLITSTLES
                     YNSFGGAIDVGALYIDEDNDINYALVIRNFGTQFTTYAGTQEKLPFEIIAGISQSVEN
                     VPIRWHITLENLQQWNVSFSNPARTQGSLDGGSEEEKVSFFNNALRHLILGAEFFPGK
                     PFNIRVGYNFRRSEELRIEDKRHFSGISAGFSLRFNRIRFDYSYSRYTIAANTSLFGL
                     TLNLQ"
     gene            complement(1882..4332)
                     /gene="lon"
                     /locus_tag="P5335_08670"
     CDS             complement(1882..4332)
                     /gene="lon"
                     /locus_tag="P5335_08670"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRZ74990.1"
                     /translation="MSNHNILTLDKLSLHDFDTEADLIPLLTPEDEEEMANEELPNSL
                     AILPLRNTVLFPGVVIPITAGRDKSIKLINDANAAGKVIGVVAQKDEDVEDPKRDDIH
                     SIGTVAQILRVLKMPDGNVTVILQGKKRFEITKVISEEPYLNALVKEVPEKRPSKTDT
                     EFGAIIESIKEMAIRIIKESPNIPTEATFAIKNIESQSFLINFVSSNMNLDVKEKQSL
                     LMMNILKERALTTLRYMNTELQKLELRNDIQSKVRVDLDQQQREYFLHQQMKTIQEEL
                     GGVSYDQEIDEMRAKAKSKKWDEKTEKHFEKEISKLQRTNPQSPDFGILRNYLELFLE
                     LPWNHFSKDIFDLKRAQKILDKDHFGLEDVKKRIIEHLAVLKLRNDMKSPILCLTGPP
                     GVGKTSIGKSIAKALGREYVRMSLGGLRDEAEIRGHRKTYIGAMPGRILQSIKKAGTS
                     NPVFVLDEIDKLSVSHSGDPSSALLEVLDPEQNSEFYDNFLEMGYDLSKVMFIATSNT
                     MSTIQPALQDRMEVIKMTGYTIEEKVEIARQHLFPKQLKEHGLTAKHLTIGKRQLEKI
                     IEGYTRESGVRALEQKLAQVIRNAAKSVAMEEEYNIKVTDEDIIKTLGAPRLERDKSE
                     GNEVAGVVTGLAWTSVGGDILFIESLISKGKGTLTLTGNLGTVMKESATIAMEYIKSN
                     EDMFGISQQVLQNYNIHIHVPEGATPKDGPSAGIAMLTSLVSVLTQKRIKKSLAMTGE
                     ITLRGKVLPVGGIKEKILAAKRANIKEIILCADNKSDIDEIKPEYLEGLTFHYVKEMS
                     EVIALAITNQKVKNAKKL"
     gene            complement(4527..5249)
                     /locus_tag="P5335_08675"
     CDS             complement(4527..5249)
                     /locus_tag="P5335_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74991.1"
                     /translation="MKKVNLFIVSFFIITSSLFSCSSEFEPIDPAVQFPDDNNDEDND
                     NGISTGNYWPMAVNNQWVFNENGLPQEPMKIISTQIIDDNTYYKYNNFVGTSTNGAPF
                     SGTVMSRKMNGVYYYRAEVIIPGTPTITVSPLEIIVLKDFLEVDETWTQSLTQTTTVE
                     GSAPITTSVQIEGKILEREASLTIGSVTYTDIIRVELVQTTQGIINKNYYWFAKDIGL
                     VKYRNVLDGTNTISELQSYTLN"
     gene            5379..5927
                     /locus_tag="P5335_08680"
     CDS             5379..5927
                     /locus_tag="P5335_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRZ74992.1"
                     /translation="MSLANENIEHLLSLCKQKNQTAQFEVYQRYCKAMYNVALRIVKD
                     EHFAEDVMQEGFLRAFLKLDDFKNEVTFGAWLKRIIINYSIDFYKKNSFYNHDDFETK
                     AFKIELKEETDDSDYSTLKVKEIHAAMNELKENYRLILNLILIEGYDYEEVCEILNIS
                     YANCRTTFSRAKESLKTKLEKK"
     gene            5930..6475
                     /locus_tag="P5335_08685"
     CDS             5930..6475
                     /locus_tag="P5335_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRZ74993.1"
                     /translation="MKKSEDPIESIFERLDNQWDTEFPNIGHADRFMEKLNRQPVQKK
                     KNNWKLFAIAASLLLTTGLITFYQYTNKPVDQWENASVQTKETHDYFASVVEKELAVL
                     KANQTKETEPIIADALKQMKVFESDYQKIIIELQKNGDTKQILYAMIVNFQTRISFLE
                     DIIKQIEIINHQNTIQNEKSS"
     gene            6459..7514
                     /locus_tag="P5335_08690"
     CDS             6459..7514
                     /locus_tag="P5335_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74994.1"
                     /translation="MKNLVNLLLLLFIAITSFAKEHNGKYTKQKTIRKTVLVNFDATV
                     SIDNKYGNMMITTWDEDKIDIEVVVTISADSEKWVDQKLASITVDFNGSKSLYSAETI
                     FDKSDNYGKSSSMEVNYTVKIPKNGSVKLDNKYGNIVMHDLNGSSDIVCKYGKITLGK
                     LNNSSNTIKIDYCSKSSIESVKNATINAKYSGLILSDFSNLDLNSSYTDVSILNGNNL
                     TYQSNYGKISVGKVTNLEGNGNYLTIKIAEIQSNLKLTTNYSNITLNAVSEKANNIAI
                     TAGYTNIKINHNPNYYFDFDITTKYANFKSDADLEYNSRIETQSSKSYKGFYKKSGVN
                     KMSIVSNYGNINLTQNL"
     gene            7535..>7937
                     /locus_tag="P5335_08695"
     CDS             7535..>7937
                     /locus_tag="P5335_08695"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRZ74995.1"
                     /translation="MKKLFLLACLFCLSTSFGQWDSKSIKGKGKFSTKEVITSDYETV
                     NVSDSFFVTLVEGNEGKINIKAEENLVEYIVVESKDGSLNIRPEKGYNLMASKGNKIE
                     ITVPIKEISKVSLAGSGDIISNFIIKTSIFKA"
BASE COUNT         2707 a         1334 c         1194 g         2702 t
ORIGIN      
        1 attaaatcca acaccaaatc aaattgttct tttttggtta aggttgaatt atcaatttca
       61 attgcatcat gagcttttac taaaggagaa tcatcacggt gggtatcaat ataatctctt
      121 tcttgaacat tttgcaaaac atcttcataa gaaacatgct gccctttttc tatcaattca
      181 tcgtgtcttc gctttgctct ggtatgagca ctcgctgtca taaatagttt caattccgca
      241 tccggaaaaa cgacagttcc aatatcacgt ccgtccatta caaccgcttt gttgttgccc
      301 attgcttgtt gttgctcaac taatttagct cttacttctg aaatttcggc tattttactt
      361 actaaacggg aaacttcaat ggtacgaatt tcgctttcaa tatttacatc attcaaaaac
      421 atttctgcaa atcctaattc gggattaaat tgaaaatgaa gttttatttc tggtaatttt
      481 ttaattaatc cttctttatc taaataatct tctgaaacca acttattttg catcgcaaag
      541 taagttactg cacgatacat tgccccagaa tcaacataaa cataacctaa atgtttcgct
      601 aattgcttgg ctaaagtact ttttccggta gatgaaaatc cgtctatggc gatggtaatg
      661 ttgttcatat tttgagtttc tgagtaacaa aattattctt ttatttactc tttttcaatt
      721 tttatcctac tgcaaattta aagttaaacc aaacaaactt gtattggctg ctatagtata
      781 tcgggaatag gaataatcaa aacgaattcg gttaaatcgc aaactgaaac cggctgaaat
      841 tccggaaaaa tgacgtttat cttcaattcg gagctcttca cttcttctga aattatatcc
      901 cactcgaata ttaaacggtt ttccgggaaa aaattctgct cctaatatca aatgtcgtaa
      961 agcattatta aaaaacgata ccttttcttc ctcacttccg ccatctaatg atccttgcgt
     1021 tcgtgccgga tttgaaaaag aaacattcca ttgctgtaaa ttttctaatg taatatgcca
     1081 tcgaatgggt acattttcaa cactttgaga aataccggca ataatctcaa acggtaattt
     1141 ttcttgagtc ccggcataag ttgtaaattg agttccaaaa tttctaatga ctaatgcata
     1201 attaatatca ttatcttcat caatatatag agctccaaca tcaatggctc ctccaaatga
     1261 attataactt tccaacgtag aggtaatcaa ttttgcattt gcacctatat aaaaatcact
     1321 aaaaggaata ttataggaat agccgaaaga taaagcaact tcactacctg taaagtctga
     1381 agttaaggct ccactttcat ctcttccttc aaaattacca taattcacat aattcacacc
     1441 ggcatgaaaa gtttgcacat gacgatcata agtataagca taagaagctg ttccgtaagt
     1501 aacttctcca taataacttg cataatttac tgaaagatga ttatgcattt cttgattgat
     1561 acttgccgga ttgaaatgag ctgcgtttac atcgtaatca aaattagtaa ttactttacc
     1621 gcccaatgca gcttgtcgtg gtgaagtgac gaggttcaaa aactgataaa cagattgtcc
     1681 accaatttgg ctgtaactta cagaacaaaa aagaacaaat agtagtgtta aaaagttttt
     1741 aaacattaga aaggaactca gttagctaaa tgataacgac aatgcgaaga tattattttt
     1801 tgttcagtaa tttttaatga aatttaaaac aataaaaaag ttggtgtcaa aaggcaccaa
     1861 cttttacaat ataaaaataa actataattt tttagcgttt ttcacttttt gattggtgat
     1921 ggctaatgct atcacttcgc tcatctcttt tacataatga aaggtcaaac cctctaaata
     1981 ttcaggtttt atttcatcaa tatcactttt gttatcagca caaaggataa tttctttaat
     2041 attagctctt ttagctgcta aaatcttttc tttaattcca cccacaggaa gtacttttcc
     2101 tctcaatgta atttcacctg tcatagctaa actcttttta attctttttt gagttaagac
     2161 agaaaccaaa gatgtcaaca tggcaatacc tgcactgggt ccatctttag gagtggctcc
     2221 ttccggaacg tgaatgtgaa tattatagtt ttgtaacact tgctgggaaa ttccaaacat
     2281 atcttcattt gatttgatat actccatagc aatagtagct gattctttca ttaccgtacc
     2341 taaatttccg gttaaggtta acgttccttt tcctttggaa atcagtgact caataaatag
     2401 tatatctcct cctacacttg tccatgctaa tccggttact actccggcta cttcattacc
     2461 ttccgattta tctctttcca aacgcggagc tccaagcgtt ttgataatgt cttcgtcggt
     2521 cactttaatg ttgtattctt cctccatggc aaccgatttg gcagcatttc gaataacttg
     2581 agctaatttt tgttctaaag ctcttacacc ggattctcgg gtatagcctt ctattatttt
     2641 ctctaattgg cgttttccaa tggttaaatg ttttgccgtt aaaccatgtt cttttaattg
     2701 ttttggaaat aaatgttgac gggcaatttc aactttttct tcaattgtat agccggtcat
     2761 tttgataact tccatcctat cttgcaaggc aggttgaatg gtagacatcg tatttgatgt
     2821 tgcgataaac atgactttag acaaatcata acccatttct aagaagttgt catagaattc
     2881 actgttttgt tcagggtcca aaacttctaa taaagcagaa gatggatcgc cggaatggct
     2941 gacagataat ttatctattt catctaatac aaaaaccgga tttgatgttc ctgctttttt
     3001 aatactttgc aatattcgtc ccggcatcgc tcctatgtaa gttttacgat gtccgcgaat
     3061 ttcagcttca tctcttaatc cacccaaaga catacgaacg tattcacgac ctaaagcttt
     3121 ggcaattgat tttccgatag acgttttacc aactcccgga ggaccggtta aacatagaat
     3181 cggtgatttc atatcatttc gcaattttaa aacggctaaa tgttctatga tacgtttttt
     3241 gacatcttcc aatccaaaat ggtctttgtc taatattttt tgagctctct ttaaatcaaa
     3301 aatgtctttc gaaaaatgat tccaaggcaa ttctaagaaa agctctaaat aattacgtag
     3361 tataccaaaa tcaggtgatt gaggattagt tcgctgtaat ttagaaattt ctttttcaaa
     3421 atgtttctct gttttctcgt cccatttctt gctttttgct ttggcacgca tttcatcaat
     3481 ttcttgatca tatgaaacac ctcctaattc ttcttggatg gttttcattt gttgatgcaa
     3541 gaaatactcc cgttgttgtt ggtctaaatc aactctaact tttgattgaa tatcatttcg
     3601 caactctaac ttttgcaact ccgtattcat atatcgcaag gtcgttaaag cacgctcctt
     3661 taatatattc atcattaaca aactttgctt ctctttcaca tctaaattca tattagagga
     3721 aacaaaattt attaaaaacg attgactttc aatattttta atagcaaaag tagcttctgt
     3781 tggtatgttt ggactttctt ttattattcg aatagccatt tctttgattg actcaatgat
     3841 ggctccaaac tctgtatccg ttttacttgg tcttttttcg ggtacttctt taactaatgc
     3901 atttagataa ggttcttcag aaattacttt tgtgatttca aatcgttttt ttccttgaag
     3961 aataaccgtt acgttaccat caggcatttt tagaacccgc aaaatttgag ctactgttcc
     4021 aatagaatga atatcatctc gttttggatc ttcgacatct tcgtcttttt gagccactac
     4081 cccaattact tttccggctg catttgcatc attgattaac ttaatggact tgtctctacc
     4141 tgcagtaatt ggaattacaa ccccaggaaa aagaaccgtg tttctaagcg gtaagatagc
     4201 aagtgaattt ggcaactctt cgttagccat ttcctcttca tcttccggtg ttaataacgg
     4261 tattaaatcg gcttctgtat cgaaatcgtg tagtgacaat ttgtcaagtg ttaagatatt
     4321 gtgatttgac atataatgtt tttagtcaag ttgtcagtta tttttttacg tctaaggcta
     4381 acaaatataa gtcatctgat tgatttgcag gtaataaaaa cctacaaaaa cgatgaaatg
     4441 catcctagcc ggtttgaata gacaaaatgc atgccataaa accgctctgt ttttaagaaa
     4501 aaaacggata tactatttat gaaaaattaa ttaagtgtat acgattgaag ttcgctaata
     4561 gtatttgtgc catctaaaac attgcggtat tttaccaatc caatgtcttt tgcaaaccaa
     4621 taataatttt tattgattat gccttgtgtt gtttgaacta gttcaactct aattatatct
     4681 gtataagtta cagaaccaat tgtcaaactt gcctctcgtt ctagaatttt tccttcaatt
     4741 tggacggaag ttgttatagg tgctgaacct tcaactgtgg ttgtttgagt taaactttga
     4801 gtccaagttt catccacttc caaaaagtct tttaaaacaa taatttctaa aggagaaact
     4861 gtaattgttg gtgtacccgg aataataact tccgcacgat aatagtatac accattcatt
     4921 tttctggaca taactgttcc tgaaaaagga gctccatttg ttgacgttcc cacaaaatta
     4981 ttgtatttgt aatatgtatt atcatctata atttgtgtgc ttataatttt cattggctct
     5041 tgtggcaatc cgttttcatt aaaaacccat tggttattaa ctgccatcgg ccaataattt
     5101 ccggtagaaa ttccattgtc gttatcctca tcattattgt catcaggaaa ttggacggcc
     5161 ggatcaattg gttcaaattc actggaacat gaaaatagtg atgacgtaat aataaagaaa
     5221 gacactataa ataggttaac ctttttcatt cgatataaaa tatttttttt caaatataaa
     5281 tatttgtact catcaactaa acgattttaa actttatttt tacaaaactg taacatttga
     5341 aaaaacaagc tgtctttaaa tcaaatcctg ttaatcaatt gagtttagca aacgaaaata
     5401 ttgagcattt actatcgctc tgcaaacaaa aaaatcaaac cgctcaattt gaagtgtatc
     5461 aacgctattg taaggcgatg tataatgttg cactcagaat tgtaaaggat gaacattttg
     5521 cagaagacgt aatgcaagaa ggatttttaa gggcttttct aaaattggat gattttaaaa
     5581 acgaagtaac ttttggtgct tggttaaaac ggattatcat caattacagt attgattttt
     5641 ataagaaaaa cagtttttat aatcatgatg attttgaaac aaaagccttt aaaatagaac
     5701 taaaagaaga aactgatgat tcagattatt caactttaaa agttaaggaa attcatgctg
     5761 ctatgaatga attaaaagag aattatcgcc tgattttaaa tttaatattg attgaagggt
     5821 atgattatga agaagtttgt gaaattctaa acatcagtta tgccaattgt aggactactt
     5881 tcagtagagc caaagaaagt ttaaaaacaa aattagaaaa gaaataaaaa tgaaaaagag
     5941 tgaagaccct atcgaatcaa tttttgaacg attagacaac caatgggata cagaatttcc
     6001 caatattgga catgcggata gatttatgga aaaattaaat cgtcaacctg ttcaaaagaa
     6061 aaaaaataat tggaaattat ttgccattgc cgcttctcta ttgttaacaa ccggtttaat
     6121 taccttttac caatatacta ataaacctgt cgatcaatgg gaaaacgcat ctgttcaaac
     6181 caaagaaaca cacgactatt ttgcttctgt tgttgagaaa gaattagccg ttttaaaagc
     6241 aaatcaaacg aaagaaactg agcctatcat tgctgacgcg ttaaagcaaa tgaaagtttt
     6301 tgaatcagat tatcaaaaaa taatcattga acttcaaaaa aatggagata caaaacaaat
     6361 actttatgcc atgattgtaa attttcaaac ccgaatttcc tttttagaag atatcatcaa
     6421 acaaattgaa atcattaatc atcaaaatac aatacaaaat gaaaaatcta gttaacttac
     6481 tcttactctt gtttattgca attacaagtt ttgcaaaaga acataatgga aaatacacca
     6541 aacaaaaaac gattagaaaa acagttttgg taaatttcga tgctacagtt tccattgata
     6601 ataaatatgg aaatatgatg ataaccactt gggatgaaga caaaattgac atagaagtgg
     6661 ttgtaactat tagtgctgat tctgaaaaat gggttgacca aaaactagcg tcaataactg
     6721 ttgattttaa tggtagtaaa agtctgtata gtgctgaaac aatttttgat aaaagtgata
     6781 attatggaaa aagtagcagt atggaagtaa attacaccgt aaaaatacca aaaaacggta
     6841 gcgtaaaatt agataataaa tatggtaata ttgtgatgca cgacttaaat ggaagttcag
     6901 acattgtgtg caaatacgga aaaatcacct taggaaaact taataattca tctaatacca
     6961 taaaaatcga ttattgctca aaatccagta ttgaatcagt taaaaatgca accataaatg
     7021 ccaaatattc cggattaatt ctttctgatt tcagcaattt agatttaaat tcaagttaca
     7081 ctgatgtttc tattttgaat gggaacaatt taacttacca aagcaattat ggtaaaattt
     7141 cggttggaaa agttacaaat ttagaaggaa acggaaatta tcttactata aagatagctg
     7201 aaattcaaag taatttaaaa ttgactacca attatagtaa tataacttta aatgctgttt
     7261 cagaaaaagc aaataatata gctataacag caggttatac aaatattaaa ataaaccaca
     7321 atcctaatta ttattttgat tttgatatca ctacaaaata tgcaaatttt aaaagtgatg
     7381 ccgatttaga atataattcc agaattgaaa cacaatccag caaatcttac aaaggctttt
     7441 acaaaaaaag tggcgtaaat aaaatgagta ttgtttcaaa ttacggaaat atcaacttaa
     7501 ctcaaaattt ataatcaatc ataaaaaaat tatcatgaaa aaattattct tattagcttg
     7561 tcttttctgt ttatcaacat cttttggtca atgggattct aaatcaataa aaggtaaagg
     7621 gaaattctct acaaaagaag ttattacatc agattatgaa acagtaaatg tttccgattc
     7681 attttttgtt accttagtag agggaaatga aggaaaaata aatatcaaag ctgaagaaaa
     7741 tttagtagaa tacatcgtag ttgaaagcaa agatggttca ttgaacataa gacctgaaaa
     7801 aggttataat ttaatggctt caaaagggaa taaaatagaa ataactgtac cgataaaaga
     7861 aatttcaaaa gttagtttgg ccggttcagg tgacataatt tcaaatttta taattaaaac
     7921 ttccattttt aaagcaa
//
LOCUS       DAOTCW010000106         7927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_6405_length_7927_cov_49.446519, whole genome shotgun sequence.
ACCESSION   DAOTCW010000106 DAOTCW010000000
VERSION     DAOTCW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7927
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6405_length_7927_cov_49.446519"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="P5335_08700"
     CDS             complement(<1..653)
                     /locus_tag="P5335_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRZ74996.1"
                     /translation="MRLLVFFILLSCSLAAQDTLTLKKNVDLKYREDQFYVGITYNTF
                     LNKPSDMAQKTFSPGINLGFLRDMPINQARTFAVATGLGISYQAYSQNLLITEDQGDY
                     NYSIIESDVSYTRNKFTMLNVDLPIEFRWRTSTPESHKFFRLYTGVKLSYLLYDESRF
                     ISNEGNEIVKNNPEVNAFQYGVYLATGYNTWNFYAYYGINTLFDSTVANIGGNPLDV"
     gene            complement(728..1201)
                     /locus_tag="P5335_08705"
     CDS             complement(728..1201)
                     /locus_tag="P5335_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRZ74997.1"
                     /translation="MSRFKKKATGDVPAISTASLPDIVFMLLFFFMVSTVMKDEDLLI
                     QNSLPKADQITKLEKKDRVMFIFAGKPHEKYRKQYGEGARIQLNDRLSDVTDIKNFIL
                     AERAARDPELEKVLTTSLKIDKDAKMGLVGEIKQELRKMDALKINYTTTKGSVID"
     gene            complement(1219..1833)
                     /locus_tag="P5335_08710"
     CDS             complement(1219..1833)
                     /locus_tag="P5335_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRZ74998.1"
                     /translation="MGRKRSAPPEVNAGSMADIAFLLLIFFLVTTTIQTDQGINRKLP
                     PIDDTERPEDNLVKEKNIFTVLINRNDQLLVEDKLMDLKDLRQAAIKFLDNGGDGTCT
                     YCRGTHDEVSSDNPEKAIISLQNDAETSYKAYIAVQNELVAAYYFLRDRECLARYNVT
                     FRELEAMFDDPKTKEAKRNKIEPLIKDIQALFPQKLSEAEPKRN"
     gene            complement(1840..2262)
                     /locus_tag="P5335_08715"
     CDS             complement(1840..2262)
                     /locus_tag="P5335_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ74999.1"
                     /translation="MNIYKLTKIVVAIIGLISVVLFFITLGQTQTIDSSVTGIFIFLS
                     YVVLFAAIIAVLYFVFRNLFKHKDELKQTLITTGLFLGIVLIAFIFADSTEVKLKDGI
                     VIGSGYSKAISTGLNTFYILAIASVGTLVFSNFKKFKK"
     gene            complement(2282..3088)
                     /locus_tag="P5335_08720"
     CDS             complement(2282..3088)
                     /locus_tag="P5335_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRZ75000.1"
                     /translation="MKKLFSILAITGLMMFGTVQAQDSTQTTEPAAVEAAAAPAAEAT
                     TEDVAAEATDETAERGFTQELKQRFIEGGPEYMGAVLLCLILGLAIAIERIIYLNLST
                     TNSKKLIKEVEEALQSGGVNAAKEVCRNTPGPVASIFYQGLDRSSHGIDSAEKSIVAY
                     GGVQMGLLEKNVSWISLFIALAPMLGFLGTVIGMIIAFDKIEAAGNMDASLVAGGIKV
                     ALLTTVFGLIVAMILQVFYNYITAKIDSIVNDMEDASISLIDILSDYLKK"
     gene            complement(3175..3262)
                     /locus_tag="P5335_08725"
     tRNA            complement(3175..3262)
                     /locus_tag="P5335_08725"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3226..3228),aa:Ser,seq:gga)
     gene            complement(3338..4366)
                     /locus_tag="P5335_08730"
     CDS             complement(3338..4366)
                     /locus_tag="P5335_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRZ75001.1"
                     /translation="MHSKANILLLYTGGTIGMMKDFETGALKAFNFKKLLQRIPELKQ
                     LDCTIETLSFEKPIDSSNMNLQRWVEMAEVIEQKYEEFDGFVILHGSDTMSYSASALS
                     FMLENLAKPVIFTGSQLPIGDLRTDAKENLITAIQIASLKQKGKSVFREVCLYFEYKL
                     YRGNRTTKINAEHFNAFRSPNFPPLAESGVHLKVNNETISTKISAKRLVVHKEMDSNV
                     VIVKMFPGLNETVLSALFEIPNLKGIILETYGAGNAPTEEWFISTLKKAIKKGIYVIN
                     VTQCSGGSVNMGQYETSTYLKKIGVISGKDITTEAAITKLMYLLGQNVSAKVFKTIFE
                     TPLRGELS"
     gene            complement(4356..5492)
                     /locus_tag="P5335_08735"
     CDS             complement(4356..5492)
                     /locus_tag="P5335_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRZ75002.1"
                     /translation="MSKFDPIRPFYDSEVNEGIRSVLHHPMMKALMNFTFPDVEESVW
                     KEQLLKTHSIRDFQVNFIYQSVQKVLQRSSEGLTTSGFEKLEKNTSYLFISNHRDIIL
                     DTSLLNVSLYDHGLVMTASAIGDNLVKKDFLLKLSRLNRNFLVLRGLPPRELLQSSKL
                     MSEYIGQLLLRENRSVWIAQREGRTKDGNDATHQGVLKMLAMGSDEENVMDYFKKIKI
                     VPVSISYEYDPTDALKMPQLMAEANNEIYIKEKNEDFITLLSGIMGQKKRIHIHVGDV
                     LEKEIDEIKLKQDNSNRQIQCLAQVIDDSILSTYRLWPTNFIAYDILHKTNKHSHLYT
                     ENEKSLFERRLELKIDNTNQVMVDSFLAMYSNPVSNKLNYHDAL"
     gene            complement(5555..6322)
                     /locus_tag="P5335_08740"
     CDS             complement(5555..6322)
                     /locus_tag="P5335_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRZ75003.1"
                     /translation="MILTDTHTHLYSDEFDSDRDEMIQRAISNGVTRFFVPAIDSTCT
                     PSMYDLEEKYPQNIFLMTGLHPCYVKENYAEELSYIENQLVQRKFVAIGEIGIDLYWD
                     KSTLEIQKIAFRHQIQLAKKYKLPIVIHCRESFDEIFEVLESEKSEELYGIFHCFSGN
                     LEQAKRAISYNMKLGIGGVVTFKNGKIDQFINQIDINELVLETDSPYLAPAPYRGKRN
                     ESSYVKLVASKLSELYGVSIEEIARITTENSKSVFGI"
     gene            6412..6852
                     /locus_tag="P5335_08745"
     CDS             6412..6852
                     /locus_tag="P5335_08745"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HRZ75004.1"
                     /translation="MKNLHAVIKKKGYKKIAFKVSKTQHLLVKATINGVKGNFILDTG
                     ASNCCIDYEFVDFFKVSAKDSKTKAAGAGATGMDTQIGKDIELQMGRWKTNKVQLVIF
                     DLTHVNLALTQYKAKPVHGIIGADVLLEGKAIIDYFNHYLYLMK"
     gene            6963..7910
                     /locus_tag="P5335_08750"
     CDS             6963..7910
                     /locus_tag="P5335_08750"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HRZ75005.1"
                     /translation="MKQKFTLTAFVLLLSALSFGQIQPFGHLTIFSEDGDKFFLILNG
                     ERINDVAQTNLRVEELVQPYYNAKIIFEDNTRNEISKNYLQVTDVDGLFMDVTYKIKR
                     DKNNNKKMKLNFFSMIPVVQGFVPPSNVYVTRWGQPAPPQQVIIQQSAPVGVVQQTTT
                     TTSTMGGVNAGVSVGGVGVNISINDPVFSGSTTVTETTTVTTGGTIVGHQEPAPVGCY
                     GARPMNNGNFSSALQTIKNQGFDESRLKTAKQIAQNNCLNTNQIVEICKIFGFEETKL
                     DFAKFAYDACVEPQNYFNVNNVFSFSSSADELNDFISRR"
BASE COUNT         2658 a         1400 c         1183 g         2686 t
ORIGIN      
        1 ctaacatcta aagggtttcc tccaatatta gctacagttg aatcaaaaag cgtgtttatt
       61 ccataataag cataaaaatt ccaagtatta tatcccgttg ctaagtaaac accatattga
      121 aaagcattaa cttcaggatt atttttaact atttcatttc cttcattaga gataaaacga
      181 gattcatcat acagtaaata acttaattta acacctgtat ataatctaaa aaatttatga
      241 ctttccggtg ttgaagtacg ccatctgaat tctattggaa ggtcaacgtt taacattgta
      301 aatttgtttc ttgtataaga aacatccgat tcaataatag aataattata atctccttga
      361 tcctccgtaa ttaataaatt ttgactatac gcttggtaag agattcccaa accggttgct
      421 actgcaaaag ttcgggcttg gttaatgggc atatctctta aaaaacctaa attaattcca
      481 ggagagaatg ttttttgagc catatcacta ggcttgttaa gaaaggtatt ataggtgatt
      541 ccaacataga attgatcctc tctatatttt aaatccacat ttttttttaa agtaagcgta
      601 tcttgtgctg ctaaagaaca acttaataaa ataaaaaaaa caagtaatcg catacgttca
      661 attttgaaag ttaaaattac aaaaaaaaag ccacgctagg cgtggctcta atatgaatta
      721 atagatttta atcaatcaca ctaccttttg tagtggtata attaattttt aaagcatcca
      781 tttttcttaa ttcttgttta atttcgccaa caagacccat cttagcatcc ttatcaattt
      841 ttaaggacgt agtaagtact ttttctaatt caggatctct agctgctctt tctgctaaaa
      901 taaaattctt tatatccgta acatctgaaa ggcgatcatt taattgtatt ctggcacctt
      961 ctccatattg tttacgatac ttttcatgag gcttacctgc aaaaataaac ataacacgat
     1021 cttttttctc caattttgta atttgatctg ctttgggcag actattttgg attaataaat
     1081 cttcgtcttt cattacagtg gacaccatga agaaaaataa caacatgaat acgatatcag
     1141 gcaacgatgc tgttgaaata gcgggaacat ctccggttgc tttcttttta aatctagaca
     1201 tttttctaaa atttaatatt aatttctttt tggctcagcc tcagaaagtt tttgtggaaa
     1261 caaagcttga atatctttaa tcaaaggttc aattttattt cttttagctt ctttggtttt
     1321 aggatcatcg aacatcgcct caagttctct aaatgtcaca ttataccttg caagacattc
     1381 tctgtctctt aaaaagtaat aggctgcaac caattcattc tgaacagcaa tataggcttt
     1441 ataggatgtt tcagcatcat tttgaagcga tataattgct ttttcaggat tatcagaaga
     1501 aacttcatca tgtgtacctc tacaataagt acaagtacca tcacctccat tatcaagaaa
     1561 tttaatggcc gcttgtctta aatcttttaa atccattaac ttatcttcaa ctaataactg
     1621 atcgtttctg ttaattaaca cagtaaaaat atttttctct tttactaaat tatcttcagg
     1681 tctttcagta tcatcaatag gaggtaactt acggttaatt ccctgatcgg tttgaatggt
     1741 cgtagtcacc aagaagaaaa tcaaaagtag gaatgcaata tctgccattg atccagcatt
     1801 tacttcgggt ggcgcacttc tctttcttcc cataattaat tattttttga attttttaaa
     1861 gttggagaac actaatgttc caacagatgc tatggctaaa atataaaaag tatttaatcc
     1921 ggtactaatt gcttttgagt atcctgaacc aataacaatt ccatctttta atttcacttc
     1981 agtactatct gcaaagataa atgctatcaa aactatacca agaaataaac ctgtggtaat
     2041 aagggtttgt ttcaattcat ctttgtgttt aaataaattt ctgaatacga aatataacac
     2101 tgcaataatt gcagcaaata atacaacata agacaaaaaa ataaaaattc cagtaacact
     2161 gctgtctatc gtttgagtct gacctaatgt gataaaaaac aataccacac ttataagacc
     2221 aataattgcg acaactattt ttgttagttt ataaatattc atttttaatt ttatttataa
     2281 attatttttt tagataatcc gaaagaatat ctatcaaact gatagaagca tcttccatat
     2341 catttacaat tgagtcaatt tttgcagtaa tataattata aaatacttgt aaaatcattg
     2401 caacaataag tccgaatacg gttgttagta acgcaacttt aatacctcct gcaaccaacg
     2461 aagcatccat atttcctgct gcctcaattt tatcaaatgc aataatcatc ccgattacag
     2521 ttcccaagaa acctaacatc ggtgccaaag caataaacaa tgaaatccaa gaaacatttt
     2581 tctctaataa tcccatttga acaccaccat aagcaacaat tgatttttca gctgaatcaa
     2641 ttccgtgaga tgaacggtca agaccttgat agaaaattga agctactggt ccaggagtat
     2701 ttctacaaac ctcttttgca gcatttactc caccagattg aagagcttct tcaacttctt
     2761 taattaattt ttttgaattt gtagtagaaa gatttaaata aataattctt tcaattgcaa
     2821 tagctaaacc aagaattaaa cataaaagta ctgcccccat atattcagga cctccttcaa
     2881 taaaacgttg ttttaattct tgtgtaaaac ctctttctgc cgtttcgtcg gtagcctcag
     2941 cagcgacatc ttccgtagtt gcttcagcag caggagctgc tgcagcttca acagctgccg
     3001 gttcagttgt ttgagtagag tcttgtgctt gaactgttcc aaacatcatt agacctgtga
     3061 ttgccaaaat agaaaataat ttcttcattg cttttgttct taattttaat tagttaatca
     3121 ggttaaaggt ataaattaat ttttaaaaaa acacacattg ataaaaaaaa tgagcggaga
     3181 gaaagggatt cgaacccttg ataccctttt ggagtataca cactttccag gcgtgctcct
     3241 tcgaccactc ggacacctct cctacattat tacaacgcat ttcaagagtg ccaaataaca
     3301 aaaaaaatct cacttggaaa aattaatttt gttattttta tgacaattct cctcgcaagg
     3361 gagtttcaaa aatggttttg aacactttag ctgaaacatt ttgtcctaat aaatacatta
     3421 atttggttat tgcagcctct gttgtgatgt cttttcctga gattactcct atttttttta
     3481 gataagtact cgtttcatat tgccccatat tgacacttcc accggaacat tgtgtcacat
     3541 tgataacgta aattcctttt tttattgctt ttttcagggt agaaataaac cactcttcag
     3601 tcggagcatt tcccgcaccg tatgtttcta aaattattcc ttttaaattt ggtatttcaa
     3661 ataaagcaga caaaaccgtt tcatttagtc cgggaaacat ctttacaatt acaacattgg
     3721 aatccatttc tttgtgaaca actaatcttt tagctgaaat tttagttgaa atagtttcat
     3781 tgttaacttt taaatgtact ccggattcag ctaaaggcgg aaaattaggc gaccgaaaag
     3841 cattaaaatg ctcagcatta atttttgtag ttcgatttcc tcggtataat ttatattcga
     3901 aatacaaaca aacttctcta aatacggatt tacctttctg tttaagtgag gcaatttgaa
     3961 tagccgttat taaattttct ttagcatccg ttcttaaatc gccaataggg agttgtgaac
     4021 cggtaaaaat gaccggtttg gctaaatttt ccaacataaa acttaatgct gaagctgaat
     4081 aggacatcgt atctgaacca tgtagaataa caaacccatc aaattcttca tatttttgtt
     4141 ctataacttc tgccatttca acccaacgtt gcaaattcat attagaagaa tctattggtt
     4201 tttcaaaaga tagtgtttca atagtacaat ccaattgttt caattctggg attcgctgca
     4261 acaacttttt gaaattgaac gcctttagag cacctgtctc aaagtctttc atcattccga
     4321 tggttccacc ggtatacaac aatagaatat ttgccttaga gtgcatcgtg ataattcaac
     4381 ttattactga ccggattaga atacattgct aaaaaactat ctaccatcac ttgatttgta
     4441 ttgtctattt ttagttctaa acgtctttcg aatagtgatt tctcgttttc tgtatacaaa
     4501 tgactgtgct tatttgtttt atgtaaaatg tcataagcaa taaaattagt aggccacaaa
     4561 cgatatgtac tcaaaataga atcatcaatt acttgggcta aacattgaat ttgacgatta
     4621 gaattatctt gtttaagttt aatttcatca atttcttttt ctaaaacatc ccctacatga
     4681 atatgtattc tttttttctg ccccataatt ccgcttaata acgtaataaa atcttcattt
     4741 ttttctttga tatagatttc gttattggct tctgccatga gttgcggcat ttttaatgca
     4801 tccgtcggat catattcgta ggagatggac accggaacaa tttttatttt tttaaaataa
     4861 tccatcacat tctcctcgtc ggaacccatg gcaagcattt ttaaaacgcc ttgatgagtg
     4921 gcatcgtttc catctttagt tcttccttca cgttgagcaa tccaaacaga acgattttca
     4981 cgcaaaagta attgaccgat atattccgac atcaatttag aactctgcaa taattctctt
     5041 gggggtaaac cgcgtaaaac caaaaaattt cggttcaacc tcgataattt taaaagaaaa
     5101 tctttcttaa ctaagttgtc cccaatggca gaggccgtca tcactaaacc atgatcataa
     5161 agagaaacat ttaataaaga agtatctaaa ataatatcgc ggtgattgga aataaaaaga
     5221 taggaagtgt ttttttctaa tttttcaaaa ccggaagttg ttaagccctc cgaactgcgt
     5281 tgcaaaacct tttgaaccga ttgatatata aaattaactt gaaaatcgcg aatagaatga
     5341 gtttttaata attgctcttt ccaaacggat tcttccacat ccgggaatgt aaaattcatc
     5401 aaagccttca tcattggatg gtgaagcact gagcggattc cttcgtttac ttcactgtcg
     5461 tagaaaggac gaatcggatc aaattttgac attttactgg tgttttaatc tacaaatgaa
     5521 caaaaaaaat gtgacttttt acagaataat ttaattaaat cccaaacacc gactttgaat
     5581 tttctgttgt aattcgtgct atttcctcaa tagaaacacc atataattca ctaagttttg
     5641 aagctactaa tttcacataa ctactttcgt tacgctttcc acgataaggt gccggagcca
     5701 gataagggga atccgtttct agaaccagtt cattaatgtc aatttgattt atgaattggt
     5761 caatttttcc gtttttaaaa gtaacaaccc cacctattcc taatttcatg ttataggaaa
     5821 tggctctttt tgcttgttct aaatttccgg aaaaacaatg aaatattcca tacaattctt
     5881 cacttttttc agattctaat acttcgaaaa tttcatcaaa tgattcccta cagtgaatca
     5941 caatgggaag tttatatttc tttgctaatt gaatttggtg tctgaaggct attttttgaa
     6001 tttccagtgt agatttatcc caatacaaat cgatgccaat ttcgccaatc gctacaaatt
     6061 ttcgttgaac caattgattt tcaatatagg ataattcttc agcataattt tctttgacat
     6121 aacaaggatg caatccggtc atcaaaaaaa tattttgtgg atatttttct tctaaatcat
     6181 acatcgatgg tgtacacgtt gaatcaatcg cagggacaaa aaaacgtgtt actccgtttg
     6241 aaatagctct ttgaatcatt tcatcacggt ctgaatcaaa ttcatcagaa tataaatgag
     6301 tatgtgtatc tgttaaaatc atcgtgcaaa aataagtaaa aaggtagata ataccggtag
     6361 cttttgtttc aatatgaatt ttaatttaca tttggaaatc aaaaggagta aatgaaaaat
     6421 ctacacgctg ttatcaaaaa gaaaggctat aaaaaaattg cttttaaagt ttctaaaaca
     6481 caacatttat tggtaaaagc gaccataaat ggcgttaaag gaaattttat tttagacact
     6541 ggtgcatcaa actgttgcat cgattatgaa tttgtagatt tcttcaaagt gagtgcaaaa
     6601 gattcaaaaa ccaaagctgc cggtgccgga gctaccggaa tggatactca aattggaaaa
     6661 gacatagaac tgcaaatggg aagatggaaa acaaacaaag tacagttggt tatttttgat
     6721 ttaacccatg tcaatttagc attaactcaa tataaagcca aacccgttca cggtattatt
     6781 ggtgccgatg tattgttaga aggaaaagca attatcgatt actttaatca ttatttatat
     6841 ttaatgaagt aagaataatt ttatttactt tcaaaacaaa ttaattttag cattattttg
     6901 gcattatttt tggaaatcta gtacctaata aactactttt aaaacctaaa atatttaaga
     6961 atatgaaaca aaaatttacc cttacagcat ttgtattatt actttcagcc ctttcttttg
     7021 ggcaaattca gccttttgga catttgacta ttttttcaga agatggtgat aaattcttcc
     7081 tgattttaaa tggagaacgc attaacgatg ttgctcaaac caaccttcgt gtagaagaat
     7141 tagtacaacc ttattacaat gccaaaatta tttttgaaga caataccaga aatgaaattt
     7201 ctaaaaacta tttacaagtt acggatgtag atggtctatt tatggatgta acatacaaaa
     7261 tcaaacgaga caagaataac aacaaaaaaa tgaagttaaa tttcttctcg atgataccgg
     7321 ttgtacaagg ttttgtccct ccatctaatg tctatgtaac gcgttggggc caaccagcac
     7381 caccgcaaca agtaattatt caacaatctg ctccggttgg tgtggtgcag caaacaacca
     7441 ctactacctc aacaatggga ggtgtaaatg cgggagtaag cgttggtggt gttggggtca
     7501 acatttctat taacgatccg gtttttagcg gaagtactac cgtgacagaa acaacaacag
     7561 taacaacagg aggtacaatt gtaggtcatc aagaacctgc acctgttggt tgttatggag
     7621 ctagaccaat gaataatgga aattttagta gtgctttgca aactattaaa aatcaaggat
     7681 ttgacgaatc aagacttaaa acagcaaaac aaattgccca aaacaattgt ttaaacacaa
     7741 atcaaatcgt tgaaatttgc aagatttttg ggtttgaaga aaccaaatta gactttgcta
     7801 agtttgctta tgatgcctgc gttgaaccac aaaattattt caacgttaat aatgtatttt
     7861 cgtttagttc gagtgcagat gaattgaatg attttatttc cagaagataa aatcaatact
     7921 ccctaaa
//
LOCUS       DAOTCW010000107         7911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_6433_length_7911_cov_39.986634, whole genome shotgun sequence.
ACCESSION   DAOTCW010000107 DAOTCW010000000
VERSION     DAOTCW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6433_length_7911_cov_39.986634"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="P5335_08755"
     CDS             <1..435
                     /locus_tag="P5335_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRZ75006.1"
                     /translation="GKPDASFEEIKIAAQQANALEFINGFPEQFETLVGERGIKLSGG
                     QRQRIAIARALLKNPSILILDEATSSLDSESEKLVQEALETLMKGRTSIIIAHRLATI
                     RSADNILVLNNGVIAEQGTHKELVEVENGIYKNLSNLQFSEY"
     gene            complement(517..1557)
                     /locus_tag="P5335_08760"
     CDS             complement(517..1557)
                     /locus_tag="P5335_08760"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HRZ75007.1"
                     /translation="MKQQIKAYNSVLDLIGNTPLIKLNRVTENLDGNFYAKVESFNPG
                     HSTKDRIALYIIEEAERQGILTPGDTIIETTSGNTGFSLAMVSIIKGYNCILAVSSKS
                     SRDKIDMLRSMGAKVYVCPAHVSADDERSYYNVAKRLHEETKGSIYINQYFNQLNVDA
                     HYKSTGPEIWEQTNGNITHLIACSGTGGTISGTAKFLKEKNPNIRILGVDAYGSVLKK
                     YHETKEFDNEEIYPYRIEGLGKNLIPTATDFDIIDKFMKVTDEESAHTTREIALKEGL
                     FVGYTSGAVVQAIRQFAEEGEFDENSNVIAIFPDHGSRYMSKVFSDDWMNEQGFFDSI
                     NMEEVQKIEFIK"
     gene            complement(1636..3696)
                     /locus_tag="P5335_08765"
     CDS             complement(1636..3696)
                     /locus_tag="P5335_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRZ75008.1"
                     /translation="MSENSIPPVAVKKDTVLEKHGDKRIDSYYWLNNRENPEVIQYLK
                     DENAYYEQMTAHTKMFQKELFEEMKSRIKEDDESVPYLYNGYYYITRFEKGKEYPIYS
                     RKKGSLTAKEEIMFDCNEFAKGKSYFKLSGLNVSEDNKLVSFATDEKGRRIYTLQVKN
                     LETGEILADKIENTTGNSVWANDNKTLFYSTQDKKTLRSDKIFKHVLGDDVKKATLVF
                     HEKDDTFATIILKTKSKKYLVINSNSTLTTEYQILNADKPQENFKVFQPRTRGLEYDI
                     SHFNDNFYIVTNKDKATNFKVMKTPEAATSKDNWVDVIPHKQDVLIEGIEIFKNYLVV
                     EERSNGLNKIKIMPWSGGGDYYLPFEIETYSAFVGTNVDFDTEILRFGYQSLATPFSV
                     IDFNMKTKTKEVKKEQAVLGGKFDKNNYKEERVWATATDGTKIPISIVYHKKTKKDGN
                     SPLLQYAYGSYGNSMEPGFDSSILSLLDRGFIYAIAHIRGGEEMGRQWYEDGKLLKKK
                     NTFTDYIDCSNFLISQKYTNSKHLYAEGGSAGGLLMGAVVNMAPQLYNGVIAQVPFVD
                     VMTTMLDETIPLTTSEYDEWGNPNDKVYYDYMRSYSPYDNVKAQDYPNMLVTTGLHDS
                     QVQYWEPAKWVAKLRTVKTDKNLLFLDTNMDAGHGGASGRFEALKETAKEFSFLLDLE
                     GITQ"
     gene            3838..4161
                     /locus_tag="P5335_08770"
     CDS             3838..4161
                     /locus_tag="P5335_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRZ75009.1"
                     /translation="MFGDLMGMMGKLKETQQKIEETKKRLDSVLIDEQSNDGLLKVTL
                     TANRLVKSIEVDPALLDDKEQLEDYLVLVMNKAIAKATSVNETELAAVAKDGMPNIPG
                     MDLFK"
     gene            complement(4171..5196)
                     /locus_tag="P5335_08775"
     CDS             complement(4171..5196)
                     /locus_tag="P5335_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HRZ75010.1"
                     /translation="MEINLVSDTVTRPNQEMLQAMFHAKVGDDVFKQDPTVNEFEAKV
                     AHLFGMEAALFFPSGTMANQTAVKLHTNPGDQIICDKWTHIHLYESGGASSNSGVNFN
                     LLDGDRGMITAEQVEGGINDPNFYHMPLSKLVCIENTTNKGGGACYEMEELHKIKQVC
                     AKHNLKYHLDGARLWNAMVAKNQSPNQYGELFDSISVCFSKGLGCPIGSVLLSDKETI
                     HRALRVRKIFGGNMRQSGYLAAAGIYALNNNIEKLADDHRRAKELESKLASCSWIEKV
                     EPVETNIIVFTPKSFVSDAVVIEKLKQKNIHISLLGKGKLRIVTHLDYKEVMHTYVLE
                     VLEKMSF"
     gene            5245..6340
                     /gene="prfB"
                     /locus_tag="P5335_08780"
     CDS             join(5245..5307,5309..6340)
                     /gene="prfB"
                     /locus_tag="P5335_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986097.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRZ75011.1"
                     /translation="MTNTEQIKGIVERLGALRRYLDIDKKLIEIANEEEKTFDPTFWN
                     NPKEAEIVVRDLRSKKKWVEDYQKGTNLAEELQMVFEFYKDGDASEEEVDSIFEQTNS
                     LIENLEFKNMLSEEGDSMSAVLQITAGAGGTESCDWASMLMRMYLMWAEKEGYKVKEL
                     NFQEGDVAGIKTVTLEIEGDYAFGYLKGENGVHRLVRISPFDSNAKRHTSFASVYVYP
                     LVDDTIEIDINPADLEIITSRSSGAGGQNVNKVETKVQLFHKPTGIQIQCSETRSQQD
                     NRQRAMQMLRSQLYEIELKKQMAQRADIEAGKMKIEWGSQIRNYVMQPYKLVKDVRTG
                     CETSDVDGVMNGEIDEFLKAYLMMMGQKEE"
     gene            6496..>7911
                     /locus_tag="P5335_08785"
     CDS             6496..>7911
                     /locus_tag="P5335_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRZ75012.1"
                     /translation="MDINFNKNEDYNKLLVAELKRRFAQVKLGGGEKRIAKLHGEGKM
                     SARERIDYLLDAKAKNIEIGGFVGDGMYVEHGGCPSGGVVVKMGYIQGKQCIVVANDA
                     TVKAGAWFPITAKKNLRAQEIAMENRLPIIYLVDSAGVYLPMQDEIFPDKEHFGRIFR
                     NNAVMSSMGITQIAAVMGSCVAGGAYLPIMSDEAMIVDKTGSIFLAGSYLVKAAIGEN
                     IDNETLGGATTHCEISGVTDYKAKDDKDALDRIKSIVGKIGDYEKAGFNREKSEKPAL
                     KVDDIYGLIPQSRSDQYDMMEVIKRLVDNSEMDLYKDGYGQSIITCYARIDGWAVGIV
                     ANQRTVSKTKKGEIQFGGVIYSDSADKATRFIANCNQKKIPLVFLQDVTGFMVGSKSE
                     HGGIIKDGAKMVNAVANSVVPKFTVIIGNSYGAGNYAMCGKAYDPRLIVAWPSAELAV
                     MGGTQAAKVLAQIEASSLKSKG"
BASE COUNT         2484 a         1419 c         1437 g         2571 t
ORIGIN      
        1 ggaaaaccgg atgcctcttt tgaagaaata aaaattgctg ctcaacaagc taatgcctta
       61 gaatttataa atggttttcc ggaacaattt gaaaccttag ttggtgaacg tggaattaaa
      121 ttatccggtg gtcaacggca acgcatagcc atcgccagag cattgttgaa aaatccgagt
      181 atcttaattt tagatgaagc cacctcgtct ttagacagcg aaagtgaaaa actggttcag
      241 gaagctctag aaaccttaat gaaaggtaga acaagtataa tcattgctca tcgcttagcg
      301 accattagaa gtgctgacaa catcttagtt ttaaacaatg gagtcattgc tgaacaagga
      361 acgcacaaag agctagtaga agttgaaaac ggtatttata aaaacttaag taatcttcag
      421 ttctctgaat actaaatcct aataccggaa aacacttaaa taaaaaactc caagcgtttc
      481 aacttggagt ttttttatat tcttttttct agtttcttat tttataaatt caattttttg
      541 aacctcttcc atattgatac tgtcaaaaaa tccttgttca ttcatccaat catcactaaa
      601 cactttgctc atgtagcgag aaccgtgatc aggaaaaata gcaattacat ttgaattttc
      661 atcaaattca ccttcctcag caaattgtct gatggcttga acaacagcac cagaagtata
      721 tcccacgaat aaaccttctt ttaaagctat ttcacgggta gtatgagcac tttcttcatc
      781 agttactttc atgaatttat caataatatc aaaatcagta gcagtgggaa ttaagttttt
      841 acctaaacct tcgatacggt aaggatatat ttcttcgttg tcaaactctt tagtctcgtg
      901 gtattttttt aatactgagc cataagcatc aactcctaga attctgatat tcggattttt
      961 ctcttttaaa aatttagctg ttccggatat tgtacctcct gtaccactac aagcaatcaa
     1021 gtgggttata tttccgttgg tttgttccca aatctccgga ccggtacttt tgtaatgagc
     1081 atcaacattt aattggttaa agtattggtt gatataaatc gaaccttttg tctcttcatg
     1141 taaccgtttg gcaacattgt aataagatcg ttcatcatct gctgaaacgt gtgccggaca
     1201 aacataaact tttgccccca tactcctcaa catatcaatt ttgtcgcgag atgattttga
     1261 gcttaccgct agtatacaat tatagccttt tataatactt accatagcta aactaaaacc
     1321 ggtgtttccg gaagtagttt caataattgt atcgccgggc gttaaaattc cttgcctctc
     1381 agcttcttca ataatatata atgcgattct atcttttgtg gaatggccgg ggttaaatga
     1441 ttcaacctta gcataaaaat taccatcaag gttttcagtg actctattga gtttaattaa
     1501 gggagtatta cctattaaat ccaatacact attataggct ttaatctgtt gtttcataaa
     1561 aaatatgtta gtcaaggcaa acttctattg tttttggcaa cctcaaaact ttgcaaatgt
     1621 ataaaaaaaa tttaattact gagttattcc ttccaaatct aataaaaaac taaattcttt
     1681 tgcagtctct ttcaatgctt caaaacgccc tgaagctccg ccatgacctg catccatgtt
     1741 tgtatctaaa aataataaat ttttatccgt ttttactgtt cgtaatttgg ctacccattt
     1801 tgccggttcc caatattgca cctgtgaatc atgcaatccg gtagtaacca acatgtttgg
     1861 gtaatcttga gcctttacgt tatcataagg tgaataggac ctcatgtaat cataatatac
     1921 tttgtcattg ggatttcccc attcgtcata ttctgaagtt gtcaacggaa tcgtttcatc
     1981 taacatggtt gtcataacat ccacaaaagg aacctgagca attaccccat tatataattg
     2041 tggtgccatg ttcactactg cccccatcaa caaacctccg gctgaaccgc cttccgcata
     2101 aaggtgtttg gaattggtat atttttgact gattaaaaag ttagaacaat caatataatc
     2161 cgtaaaggta tttttctttt tcaataattt cccgtcttca taccattgtc tacccatttc
     2221 ctccccaccg cggatatgtg caatggcata aatgaatccc ctatccaata aacttaaaat
     2281 agacgaatca aatcccggtt ccattgaatt accatatgaa ccataagcat attgcaatag
     2341 aggactgttt ccgtctttct ttgttttttt gtgataaaca atggaaattg gaattttagt
     2401 tccatccgta gcagtagccc aaacccgttc ttctttataa ttatttttgt cgaatttacc
     2461 tcctaaaacg gcttgttctt tcttcacttc tttcgttttg gttttcatgt tgaaatcaat
     2521 aaccgaaaaa ggtgtcgcta acgattgata accaaaacgc aatatttcgg tatcaaaatc
     2581 aacatttgtt cctacaaaag ccgaatacgt ttcaatttca aaaggtaaat aataatctcc
     2641 tcctccactc caaggcatta tcttaatttt attcaagcca ttggaacgct cttccactac
     2701 taaataattt ttaaaaattt ctattccttc aattaaaaca tcttgtttgt gcggaatcac
     2761 atcaacccaa ttgtcttttg atgtagccgc ttcaggcgtt ttcatcactt taaaattggt
     2821 tgctttatct ttattggtta caatataaaa attatcatta aaatgtgaaa tatcatactc
     2881 taaacccctt gttctcggtt gaaaaacttt aaagttttcc tgtggtttat cggcatttaa
     2941 aatttgatat tcggttgtta aagtactatt actgttaatt acaagatatt tttttgattt
     3001 tgttttcaga ataattgtgg cgaatgtatc atctttttca tgaaaaacta aggttgcttt
     3061 ttttacatca tctcctaaaa catgcttgaa aattttatct gaacgcaacg tttttttatc
     3121 ttgtgtggag taaaacaacg ttttgttgtc atttgcccaa acagaatttc cggttgtatt
     3181 ttcaatttta tccgctaaaa tttcacccgt ttccaaattc ttcacttgca gtgtgtagat
     3241 tcttcttcct ttttcatccg tagcaaaaga gactaattta ttgtcttcac tcacattcaa
     3301 cccgctaagt ttaaaatacg atttgccttt tgcgaattcg ttgcaatcaa acataatctc
     3361 ttcctttgca gtaagacttc ctttttttcg ggaataaatt ggatattctt ttcctttttc
     3421 aaaacgggtt atataataat aaccattata caagtatgga accgattcat catcttcttt
     3481 gattctcgac ttcatttctt caaaaagttc tttttgaaac attttagtat gagcggtcat
     3541 ttgctcataa taggcgtttt catcttttaa atattgaatt acttccggat tttcacggtt
     3601 gtttaaccaa taataagaat ctattcgttt gtctccgtgt ttttcaagaa cggtatcttt
     3661 ttttactgca acaggcggta tactattttc tgacatgttt gactcttttt ttgcacaaga
     3721 agttgcaaaa ataatacaaa ctaaactcat gactccttta taattcataa aaaaatgtta
     3781 tttcaaaact atgcaatata ctacttttgt aattcataaa ctgtaaaaat tatcaacatg
     3841 tttggagacc tgatgggaat gatgggaaaa ctgaaagaaa cccagcaaaa aatagaagaa
     3901 acaaaaaagc gtttagattc cgttttgatt gatgaacaaa gtaatgacgg acttttgaaa
     3961 gtaaccttaa ctgcaaatcg attggtaaaa tctatcgaag ttgacccggc attattagac
     4021 gacaaagaac aattggaaga ttatttagtt ttggttatga acaaagcaat agcaaaagcc
     4081 accagtgtaa acgagactga attagctgct gttgccaaag atggcatgcc gaatattccg
     4141 gggatggatt tatttaaata aagtattagt ttaaaaactc attttttcaa gcacttctaa
     4201 cacatacgtg tgcatcactt ctttataatc taagtgagtg acgattctta atttcccttt
     4261 acccaacaaa ctaatgtgaa tgtttttctg tttcaatttt tctatgacaa cagcatcgga
     4321 aacaaatgat tttggtgtaa aaacaatgat gtttgtttcc accggctcca ctttttctat
     4381 ccaagaacag ctggctagtt tgctttccag ttctttggct ctgcgatgat cgtccgctaa
     4441 tttttctatg ttattattca atgcataaat tccggctgca gctaaataac ctgattgacg
     4501 catgtttcct ccaaaaattt tccggacgcg taaagctcta tgaatagttt ctttgtctga
     4561 taataagacg gagccaatag ggcaacctaa tcctttagaa aaacaaaccg atatcgagtc
     4621 aaaaagttca ccgtattggt tcggtgattg gtttttagcg accatagcat tccacaatct
     4681 tgctccgtct aaatggtatt ttaaattgtg tttggcacaa acttgcttaa ttttatgtaa
     4741 ttcttccatt tcataacaag ctcctcctcc tttattggta gtgttttcaa tgcaaaccag
     4801 tttagacaaa ggcatatgat agaaattagg atcgtttatt cctccttcta cttgttctgc
     4861 agtaatcatg cctctgtctc catctaataa attgaaatta acgccgctat tagaggaagc
     4921 acctcctgat tcatataagt gaatatgggt ccatttatca caaataattt ggtcgcccgg
     4981 attagtatgt aatttaacag ccgtttgatt tgccatcgtt cctgatggaa aaaaaagtgc
     5041 tgcttccatt ccaaataaat gggctacttt agcttcaaac tcattgaccg tagggtcttg
     5101 tttgaatacg tcgtcaccca cttttgcatg aaacatggct tgaagcattt cttgattcgg
     5161 acgtgtaacg gtatcactaa ctaaatttat ttccataatt tgaaaaggat agcttatttt
     5221 tgcgaaaaca aaattacata atttatgaca aataccgaac aaataaaagg tattgtagag
     5281 cgtcttggtg cgttgaggag gtatctttga catcgacaag aaattaattg agatcgcaaa
     5341 cgaagaagaa aaaacctttg accctacttt ttggaacaat cccaaagaag ccgaaattgt
     5401 tgtccgcgac ttgcgttcaa agaaaaaatg ggtagaagat tatcaaaaag gaactaacct
     5461 ggcagaagaa cttcaaatgg tttttgagtt ctataaagac ggtgatgcct ctgaagagga
     5521 agtagattct atttttgaac aaactaattc cttaatagaa aacctggaat tcaaaaacat
     5581 gctttccgaa gaaggagaca gcatgagtgc cgtattacaa attactgccg gtgccggtgg
     5641 aactgaaagt tgtgattggg cttccatgtt gatgcgtatg tatttgatgt gggcggaaaa
     5701 ggaaggttat aaagtaaaag agttaaactt tcaagaaggt gatgtggccg gcataaaaac
     5761 agttacttta gaaattgaag gcgattatgc cttcggttat ctgaaaggtg aaaatggagt
     5821 gcaccgactg gttcgcatct ccccttttga cagtaatgct aagcgacata cttcttttgc
     5881 gtctgtttat gtctatccct tagtagatga caccattgaa attgacatca acccggctga
     5941 tttagaaatt attacttctc gttccagtgg tgccggtggt cagaatgtga ataaagtgga
     6001 aacgaaagta cagttattcc acaaaccgac cggaattcaa attcaatgtt cagaaacgcg
     6061 ttcgcagcaa gataaccgac aacgtgccat gcaaatgtta cgctcgcagt tgtatgaaat
     6121 tgaattgaag aaacaaatgg ctcaacgtgc cgacatcgaa gccggaaaaa tgaaaattga
     6181 atggggttca caaattagaa actatgtaat gcaaccctat aaattggtca aagacgttag
     6241 aaccggctgt gaaactagcg atgtagacgg cgtgatgaat ggagaaattg atgaattttt
     6301 aaaagcatat ttgatgatga tgggacaaaa agaagagtaa aattctaaaa tattaactag
     6361 ttagtgtttg aaactctgat ggagttaagt acattttttt taattctctt tcttcttttc
     6421 tctattttct attttctatg ttttattttc tcaatcgatt tcgtatttta gcaatcttta
     6481 ctattattac ttactatgga catcaacttc aataaaaacg aagattacaa taaactctta
     6541 gtcgcagaat taaaaaggcg tttcgcacag gtaaaactgg gaggaggcga aaaacgaatt
     6601 gccaaactgc acggtgaagg aaaaatgagt gcccgtgagc ggattgatta tttattagat
     6661 gcgaaagcca aaaacattga aattggcggt ttcgtgggtg atggaatgta tgttgaacac
     6721 ggaggatgtc catccggagg tgtggttgta aaaatgggat atatccaagg aaagcaatgt
     6781 attgttgtgg ctaatgatgc gactgtgaaa gcgggtgctt ggtttccgat tacagcaaaa
     6841 aaaaatttaa gagctcagga aatagcgatg gaaaaccgat tgccaattat ttatttggta
     6901 gacagtgccg gtgtttattt accgatgcaa gatgaaatat ttcccgacaa agaacatttc
     6961 ggacggattt tcagaaataa tgcggtgatg agcagcatgg gaattactca aattgctgcc
     7021 gtaatgggca gttgtgtggc gggtggtgca tatttgccta tcatgagcga cgaagccatg
     7081 attgttgata aaaccggaag cattttcttg gccggaagtt atttagtgaa agccgctatt
     7141 ggtgagaaca ttgacaatga aaccttaggt ggggctacaa cccattgtga aatttctggt
     7201 gtgacagatt ataaagccaa agatgataaa gatgccttag accgcatcaa aagtatcgtt
     7261 ggaaaaattg gcgactacga aaaagcaggt ttcaaccgtg aaaaatcgga aaagccggct
     7321 ttaaaagtag acgatatcta tggattaatt ccgcaatcaa ggtctgatca atatgatatg
     7381 atggaggtga ttaaacgttt ggtagataat tctgaaatgg atttgtataa agacggctat
     7441 gggcagtcta tcattacctg ttatgcaaga attgacggtt gggcggttgg aattgtggcc
     7501 aatcagcgta cggtttctaa aactaaaaaa ggagaaattc aatttggtgg cgtcatttat
     7561 tccgattcag cggacaaagc gactcgattt attgccaatt gtaatcagaa aaaaataccg
     7621 ttagtttttt tacaagacgt gaccggattt atggtgggaa gtaaatctga acatggtgga
     7681 attattaaag acggtgctaa gatggtgaat gcggttgcca attctgtggt gccaaaattt
     7741 acggttatta taggaaactc ttatggagcc ggaaactatg ctatgtgtgg aaaagcgtat
     7801 gacccacgat taattgttgc ttggcccagt gccgaattag cagtgatggg tggcacccaa
     7861 gcggctaaag tattggcaca aattgaagct tcttctctaa aatcaaaagg a
//
LOCUS       DAOTCW010000108         7724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_6707_length_7724_cov_49.103664, whole genome shotgun sequence.
ACCESSION   DAOTCW010000108 DAOTCW010000000
VERSION     DAOTCW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6707_length_7724_cov_49.103664"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="P5335_08790"
     CDS             complement(<1..431)
                     /locus_tag="P5335_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HRZ75013.1"
                     /translation="MKYFLVLFLLVFSTVGCKSKKGLFEGKASKDLATLKIIENHYEL
                     NNDYSTVYIRANAKYKDNKQSLGFSAEIKIQKDEKILVSIRFLGYTMAKGLITPTEVK
                     YYEKNGSNYFEGDYTTLSNWLGTDLDFYKVQNMLVGQAMDD"
     gene            complement(428..1780)
                     /locus_tag="P5335_08795"
     CDS             complement(428..1780)
                     /locus_tag="P5335_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRZ75014.1"
                     /translation="MRKKLFHIFLFVGILFQCNTAFSQEEPEDIALDNDEFQNSFYES
                     LREKGIENYDKAINAMQKCLQLQPNNPVVHFELGKNYFYQKEYTSAYNSFEKATQLDP
                     SNRWYWVGLYDVSYATRNYDNAIIIIKKLIEFKKEYKEDLVSLYMFTQQYDKALELIN
                     ELTETVGKTEIREQFRAQIMTATKYQGIESEKLLEAIKNNPKEESNYVALIYLYSENN
                     QEEKALEIAKLLEKEIPSSDWAQVSLFKFNLEKEDGANAALAMHKILNSPKIDQKIKH
                     RVFNEFLIFAKNNPEYTLDLEKAIHYFKDDREVKVAKEVGKFYQNKQDWPNAVKYYEL
                     FTNSNPEDLENILLLMNGYVHTNQFSILLKKSESYLELYPLQPDFYYYAGLANNQLKN
                     YKKAKEQLEIGLDYLIENKSLEANFYYQLSEAYNGLGDVKKKESYFIKAEKLLKEKKG
                     "
     gene            complement(1842..2852)
                     /locus_tag="P5335_08800"
     CDS             complement(1842..2852)
                     /locus_tag="P5335_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRZ75015.1"
                     /translation="MKIIVPMAGRGSRLRPHTLTVPKPLIPIAGKPIVHRLVEDIAGV
                     LNQQIEEIAFIIHKDFGKQVEKDLVSIAEKLGAKGTICYQNEALGTAHAIMCAKESMS
                     GPIVVAYADTLFRADFTLDTSADSVIWVKQVDDPSAFGVVQLNKHNQIVDFVEKPKEF
                     ISDLAIIGIYYFKSGETLRKELQFLLDNNVVKGGEYQLTDALENMKQKGLKFVPGKVD
                     EWMDCGNKNVTVETNTRMLNFLHQDGEKLISKVLVSENSTIIEPCFIGDNVVLKNTTI
                     GPNVSLGTGCKVENSTIKNSLVQNLTVIKNANLDNAMIGSHVSFDGNFTSISIGDYSV
                     LE"
     gene            complement(2930..3364)
                     /gene="dut"
                     /locus_tag="P5335_08805"
     CDS             complement(2930..3364)
                     /gene="dut"
                     /locus_tag="P5335_08805"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRZ75016.1"
                     /translation="MQINIINKSQHPLPNYETVASAGMDLRANLTESIILKSLERTIV
                     KTGLFIELPIGYEAQVRPRSGLAAKKGITVLNSPGTIDADYRGEIGVILVNLSNEDFV
                     IENGERVAQLVIAKHERAEWIEVTELSETSRGAGGFGSTGVK"
     gene            complement(3365..4834)
                     /locus_tag="P5335_08810"
     CDS             complement(3365..4834)
                     /locus_tag="P5335_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRZ75017.1"
                     /translation="MGIYKSLFKQTAIYGLATVLPRMFSFILVPLYTNPAVLNVQEYG
                     EVSVIFSWMVLFNVILAYGMETAFFRFYNSDNDKKSVIGTATVSIFWTTMVFLIGSLL
                     FRNKIAHITHIEVEYITYTIWILVLDALVIIPFSKLRAYQRPIAYAMVKIGNVVVNLA
                     LNLFFLLYLPKIALSNPDGFLASIYIENFQIGYIFVANVIASLLTLIVVLPNYMHIQW
                     HFDFKLWKRMMNYALPILVAGIAFAINETFDRILLDYLLPKNIAKIDVGAYSACYKLA
                     LFMTLFATAFRLGIEPFFFSHANNEEAPKTYAMITKYFIIFGSIILLAVIVFADLLKV
                     LIIRDDSYWEAMKVVPLIILANFFLGIYHNLSVWYKLTDKTKMGAYISIVGAIVTLVL
                     NFLLIANYSYVGSAIATIAAYGSMMIISYYLGNRYYPIPYDMKKILLYLGLSILFSTV
                     YFYGFRENYYVGIGFLILFIYFVYRNEKPTLLRILKKTI"
     gene            complement(5010..5429)
                     /locus_tag="P5335_08815"
     CDS             complement(5010..5429)
                     /locus_tag="P5335_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75018.1"
                     /translation="MNKLLILLLFFLLSCAPKEAFQQEFPVVISDYYYSINGNKIIFI
                     VQFEKAIPAEIQLQSLYFRNQKIAVKKKSNQNFQAVFAKPDLILDSNPEKEYGNEPPN
                     FQKPRFNLKPTEAVLEFKQAGIVKHYKFRDVVEKATN"
     gene            complement(5432..5863)
                     /locus_tag="P5335_08820"
     CDS             complement(5432..5863)
                     /locus_tag="P5335_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRZ75019.1"
                     /translation="MKLTLKELKTLPEMLEQLHLIQQLYPDYTIEIYADLLEEMLPNN
                     YKQVVVYENEQSVGLSGFWVGTKLWCGKYLELDNVIVHPNHRSRGVGKLLTEYLNKKA
                     VEIGCKVVALDAYTNNFPAHKFYYNHGFVPKGFHFVKFLEE"
     gene            complement(5958..7691)
                     /locus_tag="P5335_08825"
     CDS             complement(5958..7691)
                     /locus_tag="P5335_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ75020.1"
                     /translation="MKKYIILLLFIPLLLYGQSWQWGKRGGSLNQMSNESQREQTYFL
                     TTDSQKNIYGLSYVGATGLNIDGNVKTNHDGNPFGPSDIALFSFSCNGTYRWSKIIGG
                     NGANETVNAVCVDAQDNVYIAGRLGQCGDPNYPPRIDDDVVISQSPMDCRPMFIAKYN
                     TNGALQWIKQPQPVGTPQNEISTTSSSALLIDNAGNLNWIVLLGPGVYADGAFVNNQP
                     GVNFFILKYDTNGTYIGNTPLNIQLTGGYSTIKFYYNPYNGFYYFVGEKSDNTATAVV
                     GGTTINQFVFIACFNAQGVFQWVREDTFPDANYMFIYNLAFDPQNNIYIGGLIAGFNF
                     GSFLGFSVSETIIPGFVLKTNPDASQLLWSSYNNKGSQNRGAIALNGNELAYTSYGFG
                     VDFTWGTQTLNISGPNQGQEAILARFNKDTGACIGLTKVPGSLGFNDHGAALTVDASS
                     DYILGGYMGGTLFFDNNQQITNIGSQSDFFVAKYATEPCQPLSVADQGEKRIIMHPNP
                     TRDLVTFDNSQAGIARVSVYNYLGQEVLLPINCNGLPEVTIDMSGLSSGVYLVRLEGS
                     GVSSTVKVMRE"
BASE COUNT         2512 a         1434 c         1140 g         2638 t
ORIGIN      
        1 aaatcgtcca ttgcttgtcc gacaagcata ttctgaactt tataaaaatc taaatcagta
       61 cccagccaat tacttaaggt tgtgtaatct ccttcaaaat aattgcttcc atttttttca
      121 taatatttta cttccgtggg cgtaattaat ccttttgcca tagtgtaccc aagaaaacga
      181 atgcttacta atattttttc gtccttttga atctttattt ccgcagaaaa acccaaggat
      241 tgtttattgt ctttgtattt tgcatttgcc cgaatataaa cggtagaata atcattattt
      301 aattcataat ggttttcaat tatttttaac gtcgccaaat cttttgaagc ttttccttca
      361 aatagtcctt tttttgattt acatccaacg gtcgaaaata caagtaaaaa taaaactaga
      421 aaatacttca tccttttttt tcttttaata atttttcagc tttgatgaaa taactttctt
      481 ttttctttac atcacctaat ccattgtagg cttcacttaa ttgataataa aaatttgctt
      541 ctaacgattt attttcaata agatagtcta aacctatttc taattgctct tttgcttttt
      601 tataattttt taattgattg tttgctaatc cggcataata ataaaaatca ggttgcaacg
      661 gatataattc taaataggat tctgattttt tcaataaaat gctaaattga ttggtatgaa
      721 catatccatt cattaataaa agaatatttt caaggtcttc aggatttgaa ttagtaaaca
      781 gttcataata tttcactgcg ttaggccaat cttgtttgtt ttggtaaaat tttccaactt
      841 ctttagccac ttttacctct ctatcatctt taaaataatg aatcgctttt tccaaatcga
      901 gtgtatattc aggattattt ttagcaaaaa ttaaaaattc attaaaaaca cgatgtttta
      961 ttttttgatc aatttttgga ctattcaaaa tcttatgcat agccaatgca gcattagctc
     1021 catcttcttt ttctaaattg aatttaaaca aacttacttg tgcccaatca gaacttggaa
     1081 tttctttttc cagcaacttt gcaatttcca atgctttttc ttcttggttg ttttctgaat
     1141 ataaataaat cagtgccaca taattggatt cttctttcgg attatttttg attgcttcta
     1201 aaagtttctc tgattcaatt ccttggtatt ttgttgccgt cattatttgg gcacgaaatt
     1261 gttctctgat ttctgtttta ccaaccgttt ctgttaactc atttatcaat tccaatgctt
     1321 tatcatactg ttgtgtaaac atgtaaagcg aaactaaatc ttctttgtat tcttttttaa
     1381 attcaatcag ttttttgata atgataatcg cattatcata atttcgggtg gcataggaca
     1441 catcatatag gccaacccaa taccatcggt tagaaggatc gagttgagtt gctttttcaa
     1501 aactgttata agcacttgta tattcttttt gatagaaata atttttacct aattcgaaat
     1561 gtacaaccgg attattaggt tgtaattgca aacatttttg catcgcattg atggctttgt
     1621 cataattttc aattcctttt tcacgtaaag attcataaaa tgaattttgg aattcatcat
     1681 tatctaatgc aatatcttca ggctcttctt gtgaaaaagc agtattacat tggaataaaa
     1741 ttccaacaaa aagaaagata tgaaatagtt tttttctcat tatacctcac ccacaacccc
     1801 tctcctttgg agaggaagag cgagcacttt attaaaaatt tttattctaa aacagaataa
     1861 tcaccgatac taatactagt aaaatttcca tcaaatgaaa catgactgcc aatcatggcg
     1921 ttgtcaaggt ttgcattttt tattactgtc aaattttgaa ccaaactatt ttttatagtt
     1981 gaattttcca ctttacaacc cgtccctaac gacacatttg gtcctattgt ggtgttttta
     2041 agcactacat tatccccaat aaaacaaggt tcaatgatag ttgagttttc agaaactaat
     2101 acttttgaaa tcaatttttc accatcctga tgcaagaaat tcaacattct ggtattggtt
     2161 tctaccgtta catttttatt gccgcaatcc atccactcat ctacttttcc gggaacaaat
     2221 ttcaatcctt tttgtttcat gttttccaac gcgtcggtca attgatattc gcctcccttt
     2281 actacattat tatccaataa aaattgtaat tctttgcgaa gagtttcacc acttttaaag
     2341 taatatattc ctataatagc taaatcagag ataaactcct ttggtttttc aacaaaatct
     2401 acaatttgat tatgtttatt gagctgaaca acaccaaatg cacttggatc atccacttgt
     2461 tttacccaaa tcacactatc tgctgaagtg tctaacgtaa aatcagctcg gaaaagtgtg
     2521 tcagcatagg caacaacaat tggtcctgac atactttctt tcgcacacat gatggcatga
     2581 gcagttccca atgcctcatt ttggtagcaa atggttcctt ttgctcctaa tttttctgca
     2641 atagaaacta aatctttttc aacttgttta ccaaaatctt tatgaatgat aaaagctatt
     2701 tcttcaattt gttgatttaa tacgccggca atgtcttcca ctaatcgatg aacaattggt
     2761 tttcctgcaa ttgggattaa tggttttgga accgttaatg tatgtggtct taatcgtgag
     2821 ccgcgtccgg ccatgggaac tattattttc atgtttttta tatttaaccg taaagttcgc
     2881 aaagaattct tgcaaagttc acaaagtttt ttaattattt aacgactaac tattttactc
     2941 ctgtgcttcc aaaaccacca gcacctctgg aagtttcaga taattccgtt acttctatcc
     3001 attcagcacg ttcatgtttg gcaataacca attgagccac tcgttctcca ttttcaatga
     3061 caaaatcctc attagataaa tttactaaaa tcactccgat ttcaccacga taatcggcat
     3121 caattgttcc gggagaattc aaaacagtga ttcctttttt tgcagctaaa ccgcttcgtg
     3181 gacgaacttg tgcttcatat cctataggta attcaataaa caacccggtt tttacaatag
     3241 ttcgttctaa cgactttagt ataattgatt ccgttaagtt tgctcttaaa tccatacctg
     3301 ccgaagctac tgtttcgtaa tttggcaaag gatgttgtga cttattaata atgttaattt
     3361 gcatttatat tgtttttttc aatattcgta atagggtggg tttttcattt cgatatacaa
     3421 aataaatgaa aagtatcaaa aatccaattc caacatagta attttctcta aatccataaa
     3481 aataaacggt tgaaaacaag atggaaaggc ctaaataaag aaggattttt ttcatatcat
     3541 agggtatagg ataataacga ttccctaaat aataagatat tatcatcatg ctgccataag
     3601 ctgcaatagt ggcaatggct gagcccacat aactataatt tgctattaat aaaaagttta
     3661 atactagggt tactattgcc ccaacaatgg aaatataggc tcccattttt gttttatcag
     3721 tcaatttata ccaaacagat aaattatgat aaattcccaa aaagaaattg gctaaaataa
     3781 tcaacggaac tactttcatg gcttcccaat aagaatcgtc tcgaataata agcactttta
     3841 aaagatcggc aaatacaatt acagccaata aaataatcga accaaaaatg atgaaatatt
     3901 tggtaatcat tgcataggtt tttggagctt cttcattatt ggcgtgacta aagaaaaagg
     3961 gttcaattcc taaacgaaat gctgtggcaa atagtgtcat aaataaggct aatttataac
     4021 aggcagaata agctccaacg tcaattttag cgatgttttt tggtaataaa taatcaagta
     4081 aaattctgtc gaatgtttca ttgatagcaa atgcaatacc ggcaaccaaa attggaagag
     4141 catagttcat cattcgtttc cacaacttga aatcaaaatg ccattgaatg tgcatataat
     4201 tgggcaaaac aactatcaac gttaacaaac ttgcaattac attagcgacg aaaatatagc
     4261 caatttgaaa attttcaata tagatacttg ccaaaaaccc atcgggattt gaaagggcaa
     4321 ttttaggtaa atacaataaa aagaaaagat ttaaagcaag gtttacaacc acattaccaa
     4381 ttttaaccat cgcgtaagca atgggtcgtt gataagccct aagtttagaa aatggaataa
     4441 tcactaaagc atctaacacc aaaatccaaa tggtataagt aatatactca acctcaatat
     4501 gcgtaatgtg ggcaatctta tttctgaata aaagtgaccc aattaaaaaa accatcgttg
     4561 tccaaaaaat agaaacagta gctgttccaa tgaccgattt tttatcatta tcactattgt
     4621 aaaaacgaaa aaaagcggtt tccattccgt aagcaagaat cacattaaac aacaccatcc
     4681 aagaaaaaat aacagacact tcgccatact cctgaacgtt taaaacagct ggattggtgt
     4741 aaagaggtac taaaataaag ctgaacattc tgggaagcac agttgctaac ccataaatgg
     4801 ccgtttgttt aaaaagtgat ttataaatac ccaaaagaat tctttagttt tattgctcaa
     4861 atttagttta aaaaaatgta aagccattga ttcattggtt ttaaatttta tcgaatctcc
     4921 gtgtgaaaaa ttagtgtttt gcttatttta caatgtttca caaattctat agaataaaaa
     4981 aacaacattg tttttgacgt ttgattctgc taatttgtgg ctttttcaac tacatctcta
     5041 aatttataat gttttactat tccggcttgt ttaaattcta aaacagcttc ggtaggtttt
     5101 aagttaaatc tgggtttttg aaaattaggt ggttcattac catattcttt ttctggattt
     5161 gaatctaaaa ttaaatcagg tttagcaaaa acggcttgga aattttgatt ggattttttt
     5221 tttactgcta ttttttggtt tctgaaataa agtgattgaa gttgaatttc tgcaggaatt
     5281 gctttttcaa attggacaat aaaaataatt ttatttccat tgattgagta ataatagtca
     5341 gaaatcacaa caggaaattc ttgctggaat gcttctttgg gagcacagct aagaagaaag
     5401 aacagtagta agattagtaa tttattcatt attattcttc caaaaattta acgaaatgaa
     5461 acccttttgg cacaaaacca tggttgtaat aaaatttatg ggcaggaaaa ttattggtat
     5521 aagcatctaa agcgaccact ttacaaccaa tttcaacagc ttttttgttt aaatattccg
     5581 ttagcaattt accaacgcct ctagaacgat gattgggatg tacaattacg ttatcgagtt
     5641 ctaaatattt tccgcaccat aatttggttc ccacccaaaa accagacaag cccaccgatt
     5701 gctcgttttc ataaaccaca acttgtttat agttatttgg aagcatctct tcgagtaaat
     5761 cagcataaat ttcgattgta taatcaggat ataattgctg aattaagtgc aattgctcca
     5821 gcatttcggg tagcgttttg agttctttaa gtgtcaattt tatcaaaatt ttatttagtt
     5881 taaagataaa aaaaagagcc aacggttgtt aactcttttt ataaaaatat atagttacat
     5941 ttaaaaatat ccactcttta ctcccgcatc acttttactg tactactaac gcctgatcct
     6001 tccagtcgta ccaagtaaac accacttgat aatccactca tatcaatagt aacttcgggt
     6061 agtccattac aatttatagg taacaatacc tcttgtccca aataattata aactgacacg
     6121 cgtgctatac ccgcttggct attatcaaaa gttactagat cacgtgtagg atttgggtgc
     6181 attataatgc gtttttcgcc ttgatccgct actgataacg gctgacaagg ctccgtagca
     6241 tacttagcca caaaaaaatc cgactgcgaa cctatattgg tgatttgttg gttattgtca
     6301 aaaaacaacg ttcctcccat ataaccccct aagatataat cgctagaggc gtccacagtt
     6361 agtgccgcac catgatcatt aaaacccaaa ctacctggca ctttcgttag ccctatgcaa
     6421 gcaccagtgt ctttgttaaa ccgtgctaat attgcttctt gaccttgatt aggtcctgaa
     6481 atattcaagg tctgcgtgcc ccaagtaaaa tcgacaccaa aaccataact agtgtaagcc
     6541 aactcattgc cattaagtgc tatggcacct ctgttttgtg atcctttatt gttatacgaa
     6601 ctccaaagca gctgactagc atcaggattg gtcttcaata caaatccggg tataatagtc
     6661 tcagaaactg aaaaacctaa aaaactacca aagttaaaac ctgctattaa acctcctatg
     6721 taaatattgt tctgtgggtc aaaggcaagg ttataaataa acatataatt agcatcagga
     6781 aacgtatctt cacgcaccca ttgaaaaacc ccttgtgcat taaaacatgc tataaaaaca
     6841 aattgattga tagtagtacc cccaacaaca gccgttgcag tgttatcgct tttttcacct
     6901 acaaaataat aaaacccatt ataggggtta tagtaaaact taattgtgct ataacctcct
     6961 gttaattgaa tgtttaatgg tgtattgcca atataagtac cattggtgtc atattttaaa
     7021 atgaaaaaat taacccctgg ctggttgttc acaaaagccc catcagcata aacacccgga
     7081 cccaatagaa caatccagtt taaattcccc gcattatcaa tcaataatgc acttgatgag
     7141 gtggtgctta tttcattttg tggtgtacca acaggttgcg gttgcttaat ccattgcaaa
     7201 gccccattcg tgttatattt ggctataaac ataggcctac aatccatagg gctttgtgaa
     7261 ataaccacat catcatctat tcgtggggga tagttggggt caccacactg gcctaaccgt
     7321 cctgctatat acacattgtc ttgtgcgtct acacacacgg cattcacggt ttcattagca
     7381 ccattaccac cgataatctt cgaccagcgg taagtaccat tacacgaaaa actaaacaag
     7441 gcaatatctg aaggtccaaa aggattacca tcatgattcg tttttacatt accgtcaatg
     7501 ttcaagccag tagcccctac gtaggacaag ccataaatat tcttctgact gtcggtagtt
     7561 aaaaaatagg tctgctcacg ttgactttca ttgctcatct ggttaagact acctccacgc
     7621 ttcccccact gccacgattg gccgtagagt agcagaggga taaacagcag taggattata
     7681 tactttttca tagcttattg ttttaccatt tttatttgtt ttaa
//
LOCUS       DAOTCW010000109         7721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_6715_length_7721_cov_37.635664, whole genome shotgun sequence.
ACCESSION   DAOTCW010000109 DAOTCW010000000
VERSION     DAOTCW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6715_length_7721_cov_37.635664"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="P5335_08830"
     CDS             complement(<1..528)
                     /locus_tag="P5335_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HRZ75021.1"
                     /translation="MEEKKLDVNSIIGFGLIFVILIWVMYNSQQKEAAAQVKKAQQEQ
                     VEAKANPTQAKVVSTTEPETQKPVSDSVQVTQLKSSLGSFAYSATLPSAKAAFTTIEN
                     ELVRLKIANKGGYIVEAEIKQFDQFTKDSGKKVQLIKDGNANFNIELKTNDNRTLNTK
                     DLFFEPVLTKEGTNQV"
     gene            complement(562..2181)
                     /locus_tag="P5335_08835"
     CDS             complement(562..2181)
                     /locus_tag="P5335_08835"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRZ75022.1"
                     /translation="MNQTKYIFVTGGVTSSLGKGIIAASLAKLLQARGYRTTIQKFDP
                     YINVDPGTLNPYEHGECYVTDDGAETDLDLGHYERFLNVPTSQANNVTTGRVYLSVIE
                     KERRGEFLGKTVQVVPHITNEIKERMQLLGNSGDFDIVITEIGGTVGDIESLPYIESV
                     RQLLWELGDENAIVIHLTLIPYLAAAGELKTKPTQHSVKTLMESGIQADILVCRTEHE
                     LSDELRQKLALFCNVKREAVIQSIDASTIYDVPNLMLEEGLDKVALKKLNLPEKAAPD
                     LKQWNDFLFKLKNPKHTVNIGLVGKYVELQDSYKSILEAFIHSGAANETKVNIVSVHS
                     EHIDKTNVAEKLKDFDGILVAPGFGERGIEGKIETVRYVRENKVPFFGICLGMQMAVI
                     EYARTVLGYTDANSTEMNSKTSHPVIDLMEEQKTITDKGGTMRLGSWKCNLEPNSLAH
                     KIYGKSQIAERHRHRYEYNSGYREQLEKAGLKASGINPETGLVEIVEIENHPFFIGVQ
                     YHPEYKSTVANPHPIFVGFVKAAVKHKAVSN"
     gene            complement(2331..2561)
                     /locus_tag="P5335_08840"
     CDS             complement(2331..2561)
                     /locus_tag="P5335_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HRZ75023.1"
                     /translation="MEDQQKILIKLAHTKMPFGKYEGYYLIDLPEYYVVWYHTKGFPK
                     GTLGEQMQLIYELKLNGLEELVRTIRKKFPKP"
     gene            complement(2627..3028)
                     /locus_tag="P5335_08845"
     CDS             complement(2627..3028)
                     /locus_tag="P5335_08845"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRZ75024.1"
                     /translation="MTSKVTYIGELRTTSIHLQSGTEILSDAPKDNHGKGEAFSPTDL
                     VANSLATCMISIMGIKSKDLNVDLSGSTAEVTKIMQAEPRKIAKIEVVFNMTIAPDDK
                     TKIILERAAMTCPVLLSLHPDIEKEITFNWK"
     gene            complement(3028..>4884)
                     /locus_tag="P5335_08850"
     CDS             complement(3028..>4884)
                     /locus_tag="P5335_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRZ75025.1"
                     /translation="CAINLLFAQPTNYKKHTVQKGETITQIAQKYKVTPYDIYRLNPD
                     AQNGIQENAILLIPSALLVTNQTTSAKTHEVQPKETLFGIAKEYNMSVEELEKLNPEV
                     KEGLKIGQLIQLKPQKAKAPLGEINQKQVLYHEVQPKETLYSIAKQYNTTVEILETLN
                     PAVKSGLSIGQQLIIGNGKIAKNDNGTKVEPKPKAINYLTYEVKPKETLFGLSKMFRM
                     TQEELIVLNPELKEGVREGMVIKVPANVSLNSGENNEIKDLTKSISNSGRKELVLLLP
                     FNISTIESDTTLSTQDRLKRDGFLNMTLDFYSGALMAIDSVKRLGLNFNVKIIDSKET
                     KSSSAISSIIRSNNMENADAVIGPFYPQYVEQTAELLNASNVPVISPLRETSKSYSNL
                     FQSMPSSDLVKNTMFDFIRAKNGNMIALIDKKKLASKQYIEVSHRDVFIAPLNENGTV
                     IADSITPRLVSNKTNYFILETASTGMIFSAISQCNTARTNGFNVELVVLDINSTFESD
                     EVFQKVVKQKIIFPSLTKYQETPESIRFASNYKKINNVNPNQYAIRGFDVVFDTMLRL
                     SQAESFEQTIQNTATEGVENKFDYEKKLASGYTNKGVYIMYYDEDLTVKTAE"
     assembly_gap    4885..4984
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4985..5469)
                     /locus_tag="P5335_08855"
     CDS             complement(<4985..5469)
                     /locus_tag="P5335_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRZ75026.1"
                     /translation="MKKFLFFAMFLLASQITFSQTAIKPGMNAINHKVASGETVLDIS
                     KKYGVDPAEIYRANRFAIDGIKEDMVLQFYAPQKPSEIEISTENYKTKPETVSAEIIP
                     EKSIEQPIAVAPISAKVTNRGYHTVQKGETLYGLSKMYNVTVDDIKNSNTILMKNSLQ
                     AG"
     gene            complement(5485..7014)
                     /gene="guaA"
                     /locus_tag="P5335_08860"
     CDS             complement(5485..7014)
                     /gene="guaA"
                     /locus_tag="P5335_08860"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRZ75027.1"
                     /translation="MQHNVLILDFGSQYTQLIARRVRELNIFCEIFPYNHVPEDLSSF
                     KATILSGSPFSVRAEDAPHPDLSQIRGKMPLLAVCYGAQYLAHFDGGEVAPSNTREYG
                     RANLSFVDDHDPFFEEVALNSQVWMSHSDTIKKLPTNGVRLASTKDVENAAYRIEGEK
                     TYAIQFHPEVYHSSDGKQMLENFLVRIAEVPQNFTPNAFVEEIVAELQDKIKDDKVVL
                     GLSGGVDSTVAAVLLHKAIGKNLYCIFVNNGLLRKNEFQNVLDQYKGMGLNVKGVDAA
                     DRFLDQLEGIEEPELKRKTIGRVFIEVFDDEAHRIEEVKWLAQGTIYPDVIESVSVKG
                     PSATIKSHHNVGGLPDFMKLKIVEPLRMLFKDEVRRVGATLGIDPELLGRHPFPGPGL
                     SIRILGDITKEKVRILQEVDAIFIEGLKSHGLYDKVWQAGAILLPVNSVGVMGDERTY
                     EKVVALRAVESTDGMTADWVHLPYDFLMKISNEIINKVKGVNRVVYDISSKPPATIEW
                     E"
     gene            complement(7110..>7721)
                     /locus_tag="P5335_08865"
     CDS             complement(7110..>7721)
                     /locus_tag="P5335_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRZ75028.1"
                     /translation="RNVTKNPDDGFALTGKVELFPLGAFTKDGTNFEGDIVRESKPKL
                     LLSGVFHRNVHARLTQGQLGAELYEKKTMESVFVDAMFKYNGWAFMSAYMNRTSNDPI
                     AINPDDATQFNYVYVGHGMDYQLSYIFLNKLEVIGRYSNQKVNDDIYLYTPNSKVYTL
                     GLTKYIWEHAFKLQAEVTYDELSYFNGDVKNNLYVRFQIEIGI"
BASE COUNT         2267 a         1420 c         1198 g         2736 t
ORIGIN      
        1 tacttgatta gttccttctt tggttaaaac cggttcaaaa aataagtctt tcgtatttaa
       61 agtgcggtta tcatttgttt ttaattcaat attaaaattg gcatttccat ctttaataag
      121 ttgaactttt ttaccggaat ctttcgtgaa ttgatcaaac tgttttattt ccgcttcaac
      181 gatataaccc cctttgtttg caatttttaa acgtaccaat tcgttctcaa tagttgtaaa
      241 agcagctttt gcagaaggaa gtgtggcaga ataagcaaaa gaacctaaac tactttttaa
      301 ttgagtcacc tgaacagaat cagaaaccgg tttttgagtt tcaggttctg ttgtagaaac
      361 tacttttgct tgcgttggat tcgccttggc ttctacttgt tcctgttgag cttttttaac
      421 ttgtgctgca gcttcttttt gttggctgtt gtacattacc caaattaaga ttacgaatat
      481 taatccgaag ccaatgatgg agttaacgtc taattttttt tcttccattt aaattaattg
      541 tttctagttc aatgtttcaa attagtttga aactgcttta tgcttaactg cagctttaac
      601 aaatccaaca aaaattggat gagggtttgc tactgtactt ttatattccg gatggtattg
      661 aacgccgata aaaaacggat ggttttcgat ttcaacaatc tcaactaaac cagtttccgg
      721 attgatacca ctcgctttta atcctgcttt ttctaactgt tcacgataac cactattgta
      781 ttcataccga tggcgatgac gttctgctat ttggcttttg ccataaattt tgtgagctaa
      841 ggaattaggt tctaagttgc atttccaact tcctaatcgc atcgttccac ctttatcagt
      901 aatcgttttt tgttcttcca tcaaatctat caccggatgt gaggttttag aattcatttc
      961 ggttgaattg gcatcagtat atcccaaaac cgttcgagca tattctatta cggccatttg
     1021 cattcctaaa caaataccga aaaacggaac tttattttca cgaacgtaac gaactgtttc
     1081 aatttttccc tcaattccac gttcaccaaa tccgggagcg accaaaatac catcaaaatc
     1141 tttcaatttt tcagccacat ttgttttatc aatatgctca gaatgaacgc tcactatatt
     1201 tactttcgtt tcatttgctg ctcctgaatg aataaaggct tctaaaattg atttatacga
     1261 atcctgtaat tctacatatt ttccaactaa accaatattt acggtatgtt ttggattttt
     1321 taatttaaac aaaaaatcat tccattgctt taaatcgggt gctgcttttt cgggcaaatt
     1381 aagctttttc aacgctactt tatccaaacc ttcttctaac atcaaattgg gaacatcata
     1441 aatggtagag gcatcaatcg actgaataac cgcttcacgc tttacattac aaaacaacgc
     1501 taatttttga cgtaactcat ctgataattc atgctccgtt cgacaaacca aaatatcggc
     1561 ttgtataccg ctttccatca atgttttaac agaatgttgc gttggttttg ttttcaattc
     1621 acctgcagcg gccaaataag gtatcaaggt taaatgaatc acaatagcgt tctcatctcc
     1681 taattcccat aacaattgac ggactgattc aatgtagggt aatgattcga tatcacctac
     1741 agttcctcca atttcagtaa ttacgatatc aaaatcccct gaattaccca gcaattgcat
     1801 tcgttcttta atttcgttgg taatgtgggg gactacttgc actgttttac ctaaaaattc
     1861 acctcttcgc tccttttcaa tcacagaaag atatactctt ccggtagtta cattatttgc
     1921 ctgcgaagtg ggtacgttta aaaaacgttc gtaatgacct aaatccaaat cggtttctgc
     1981 tccatcatca gtgacataac attctccgtg ttcataggga tttaatgttc ccggatctac
     2041 attaatgtaa ggatcaaact tttgaatggt cgttcgatat cctcttgcct gcagcaattt
     2101 tgccaacgaa gctgcgataa ttccttttcc taaggaagaa gtcacgccac cggtaacaaa
     2161 aatatacttt gtttgattca ttgtgttgtt gtttgtagtt gtagttgtgt gcctaaattg
     2221 aaatcgttta cctcaattta aaacggggca aaaatacgat tataatttat caaattgaaa
     2281 attaatcgca gtgaaaaata aatttttaga agataaaacg caaagtcaaa ctaaggtttt
     2341 ggaaattttt tacgaatggt tcgaacaagt tcttctaatc cattcaattt gagctcataa
     2401 atcaattgca tttgttctcc taatgttcct tttggaaaac cttttgtgtg ataccatacc
     2461 acataatatt caggaaggtc tatcaaataa taaccttcat acttaccgaa aggcatttta
     2521 gtatgggcta atttgataag aattttttgt tggtcctcca tcaataatgt atgtttccaa
     2581 ttgttggtta taggatgtca gccttctatt tatagacaac agataattat ttccaattaa
     2641 aggtaatttc tttttctata tcaggatgta aactcaatag aacgggacaa gtcatggctg
     2701 ctctttctaa aatgattttt gttttatcat caggagcaat tgtcatgtta aaaactactt
     2761 caattttggc gatttttctt ggttctgctt gcataatctt tgttacttcg gccgtagaac
     2821 ctgataaatc aacgtttaaa tcttttgatt ttattcccat gattgaaatc atacaagtag
     2881 ctaatgagtt ggctactaaa tccgttggcg aaaaagcttc tcctttaccg tgattatctt
     2941 taggagcatc tgataaaatt tctgttcccg attgaagatg aatggaagtt gttcttaatt
     3001 ctcctatata ggttacttta gaagtcatta ttctgctgtt tttactgtta aatcttcgtc
     3061 gtaatacatt atgtaaacgc ctttgtttgt gtaacccgat gctaattttt tctcgtaatc
     3121 aaatttattt tcaacccctt ccgtagctgt attttgaatg gtttgttcaa aactttcggc
     3181 ttgagataaa cgcaacattg tatcaaaaac tacatcaaaa ccacgaatgg catattgatt
     3241 tggatttaca ttattaattt ttttataatt actcgcaaaa cgaatcgatt caggcgtttc
     3301 ctggtatttt gttaaggatg gaaaaatgat tttttgtttt acaacttttt ggaatacttc
     3361 gtctgattca aaagtactat taatatctag cacaaccaat tctacattga aaccattagt
     3421 acgagctgta ttgcattgag atatagcact aaatatcatt ccggtgcttg ctgtttctaa
     3481 aataaaatag tttgttttat tgcttaccaa tctaggcgta atactatcag ctatgactgt
     3541 gccattttca ttaagtggag caataaaaac atcgcggtga ctgacttcaa tatattgttt
     3601 tgaagccaat ttctttttat caattaatgc aatcatattt ccattttttg ctcttatgaa
     3661 atcaaacatg gtatttttta ccaaatcact ggaaggcatg gattggaata aatttgaata
     3721 tgattttgaa gtttctctta atggagaaat aaccggcacg ttactcgcgt ttaaaagttc
     3781 ggcggtttgt tctacatatt gtgggtagaa tggtccaatg actgcatcgg cattttccat
     3841 attattactt cggataattg atgaaatagc tgaagaactt tttgtttcct tagaatcgat
     3901 tattttcaca ttgaaattta atcctaatcg tttcaccgaa tcaatagcca tcaaagcacc
     3961 cgaataaaag tctaatgtca tattcaaaaa cccatctcgt ttcaatcgat cctgagtgga
     4021 caatgtcgta tcactttcaa ttgtcgaaat attaaaagga agtaataata ccaattcttt
     4081 ccttcctgaa ttagaaattg acttagttaa atctttaatt tcattatttt ctccactgtt
     4141 caaagaaaca ttagcaggaa cttttatgac catgccttct cttacgcctt cttttaattc
     4201 tggatttaaa acgattaatt cctcctgagt cattctaaac attttagata atccaaatag
     4261 tgtttctttt ggtttaactt catatgttag ataattaatt gcttttggtt tgggttctac
     4321 tttagttcca ttatcatttt tagcaatttt tccgttacca ataattaact gttggccaat
     4381 agataaccca cttttgacag ccggattcaa cgtttctaaa atttctaccg ttgtattata
     4441 ttgtttagct atactgtaaa gcgtttcttt aggttgtact tcatgataaa gaacttgttt
     4501 ctgattaatt tcacctaagg gggcttttgc tttttgaggt ttcagttgaa ttagttgacc
     4561 tatttttaat ccttctttga cttcagggtt taatttctct aactcttcaa cagacatatt
     4621 atattcctta gcaattccga aaagagtttc tttcggttgc acttcatgcg ttttggcgga
     4681 agttgtttga tttgttacca ataaagcaga aggaattaac aagatggcat tttcttgaat
     4741 accgttttgt gcatcaggat ttaaacgata aatatcgtac ggtgtaactt tatatttttg
     4801 agcaatttga gtaatcgttt cccctttttg aaccgtatgt tttttataat ttgtaggttg
     4861 tgcaaaaagt aaatttatgg cacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnccggct tgcaaactgt ttttcattaa aatggtattg ctgtttttaa tatcatctac
     5041 cgttacatta tacattttag acaatccata taatgtttcc cctttttgaa cagtatgata
     5101 acctcgattg gtgacttttg cagaaatagg agcaactgca attggctgtt caatagactt
     5161 ttccggtata atttcggcag aaacagtttc aggttttgtt ttataatttt ctgtgctaat
     5221 ttcaatttca gatggttttt gtggtgcata gaattgtaaa accatgtctt cttttattcc
     5281 atcaatagca aaacgattgg ccctgtaaat ttctgcgggg tcaacaccat actttttaga
     5341 aatgtctaaa actgtttcgc cggatgcaac tttgtgattg attgcattca ttccgggttt
     5401 aatcgcggtt tgtgaaaaag tgatttggga ggctagcaag aacattgcaa aaaacaaaaa
     5461 cttcttcatt ttaaataact taaattattc ccactcaatc gttgccggtg gctttgaact
     5521 gatatcataa actacccgat taacaccttt tactttattt ataatttcat ttgaaatttt
     5581 cattaagaaa tcataaggca aatgtaccca atctgcagtc attccatcag tcgactcaac
     5641 ggctctcagg gcaactactt tttcataagt acgttcatca cccattaccc ctacactatt
     5701 cacaggaagc aatatggctc cggcttgcca cactttatca taaagtccgt gggattttaa
     5761 accttcaata aaaatggcat caacttcttg caatatacga actttttctt ttgtgatatc
     5821 gcctaaaatt ctgatagata atcctggccc cggaaaaggg tgtcttccca ataattccgg
     5881 atcaattcct agtgtagctc ctactcttcg cacttcatct ttaaatagca ttcgaagcgg
     5941 ttctacaatt tttaatttca taaaatcagg caatccgcct acattatgat gtgatttaat
     6001 tgttgctgaa ggtcctttta cggaaactga ttcaatgaca tcaggataaa tagtaccttg
     6061 agcaagccat ttcacctctt caattcggtg agcttcatca tcaaaaactt ctatgaaaac
     6121 acgcccaatg gtttttcttt tcaattcagg ttcttcaatt ccttctaatt ggtctaaaaa
     6181 gcggtcagca gcatctactc cttttacgtt taagcccatt cctttatatt ggtctaatac
     6241 attttggaat tcattttttc ttaacaaacc gttattcaca aaaatacaat agagattttt
     6301 cccaatggct ttgtgcaata aaactgcagc cacagtagaa tctactccac cggaaagacc
     6361 taaaacaact ttatcgtctt ttattttatc ttgcaattca gcaactattt cttccacaaa
     6421 tgcattgggt gtgaaatttt gaggcacttc tgctatacga accaaaaagt tttctaacat
     6481 ttgttttcca tctgacgaat gataaacttc cggatgaaac tgaatcgcat aggttttttc
     6541 accttcaatt cgataagccg cattttcaac atctttagtg cttgccaaac gaacgccgtt
     6601 agttggtaat ttttttatgg tatcactgtg gctcatccaa acttggctat tgagagccac
     6661 ttcttcgaaa aaaggatcat gatcatctac aaaactcaag ttagctctgc catattcacg
     6721 agtattagaa ggagctactt cacctccatc aaaatgtgct aaatattgtg caccgtaaca
     6781 aacggctaat aatggcattt taccacgaat ttgggacaaa tcaggatggg gagcatcttc
     6841 agctctaact gaaaaagggc ttcccgaaag aatagttgct ttaaaactgg ataagtcttc
     6901 aggaacatgg ttgtaaggaa aaatttcgca gaatatgttt aattcccgaa ctcttctcgc
     6961 aataagttgt gtgtattgcg aaccgaaatc taatataagt acgttgtgtt gcatgggcaa
     7021 aaatagggat attcttggaa agtgaaaaac agaaaagagg ttaaatttaa taaaaattca
     7081 acctctcatt gtttttgtat tgtgattagt taaattccga tttcaatttg gaaacgaaca
     7141 tacaaattat ttttaacatc tccgttgaaa taagaaagtt cgtcataggt tacttccgct
     7201 tgaagtttaa aagcatgttc ccatatatat ttagttaacc ctaacgtgta gactttagaa
     7261 tttggggtat acaaataaat atcatcattt actttttgat tagaatacct gccaataact
     7321 tcaagtttgt ttaaaaaaat ataacttaat tgataatcca tcccatgacc tacataaaca
     7381 taattaaatt gtgttgcatc atcgggattg attgcaatgg ggtcatttga agttctgttc
     7441 atataagcag acatgaatgc ccaaccattg tatttaaaca tcgcatcaac aaaaacagat
     7501 tccatggttt tcttttcata caattcagcc cctaattgtc cctgagtcaa tcgggcatgc
     7561 acatttctgt gaaaaacccc tgaaagcaat aattttggtt ttgattctct cacaatatcc
     7621 ccttcaaaat ttgttccgtc ttttgtaaaa gcacctaatg gaaatagttc tacttttccg
     7681 gttaaagcga atccatcatc cggattttta gttacatttc t
//
LOCUS       DAOTCW010000110         7510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_7006_length_7510_cov_39.610329, whole genome shotgun sequence.
ACCESSION   DAOTCW010000110 DAOTCW010000000
VERSION     DAOTCW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7006_length_7510_cov_39.610329"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1090)
                     /locus_tag="P5335_08870"
     CDS             complement(2..1090)
                     /locus_tag="P5335_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRZ75029.1"
                     /translation="MRILKYILLLIGLVALALVVYIATKDGSYSVTSSRVIKLEKSTL
                     FQYVNDYKNWEDFCSLKEDDTQLKFTFPENTVGVGGAFSWDGGLESGKMQTIYLKEND
                     SIAQNISWNGTESKANIIFRDTIGGTKVRWSCKGNQSFLGKFYALFSGGIEKKLTQNF
                     ENSLAKLDKVLTKEINTYDISINGLVEVNKITYLKRRSVSKIPDFYTKVQKQMPLLLN
                     FVKDNNVATFGSPFVAFDSYDIPNNLVKFAIAIPLKEEIFTTPESEFITDSILPYQAV
                     KITLKGDYSHSKEAWDKGTEYIKQNNLEESATGTYQEVYTKSRKDIKYPSQWITEIYI
                     PVKAKPFSKPAPEPTLPKPKMDIPEAIE"
     gene            complement(1346..1957)
                     /locus_tag="P5335_08875"
     CDS             complement(1346..1957)
                     /locus_tag="P5335_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRZ75030.1"
                     /translation="MIRIAIAEDHQALLDGIKLMLEFETDIEVVGEANDGEELLEIVK
                     SKQPDLVLTDIRMPKCDGLCATKAIMALYPNTRIIGFSMFEQYEAVQQMKAAGAKGYI
                     LKNSSLKKVLEAIRTVASGKTYFDNDIITHDSITKEEIILSNREKEILQLIGEGKTSN
                     EIATSLFISKTTVDSHRKNIMKKINIHGKTDLIRFAVERKYDF"
     gene            complement(2126..2317)
                     /locus_tag="P5335_08880"
     CDS             complement(2126..2317)
                     /locus_tag="P5335_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75031.1"
                     /translation="MFSKGQLIFAGIFVVCFIAIMIYVYRKDFALHKIHYKGSLKVLF
                     GFILFIILLFIIKIYLKNK"
     gene            2416..2799
                     /locus_tag="P5335_08885"
     CDS             2416..2799
                     /locus_tag="P5335_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HRZ75032.1"
                     /translation="MQKQIHAVKEFHEAFEIGFKESPAVHLGENKNLLRYNLMKEENE
                     EYLEAAMNNDLVEVADALGDMLYILCGTIIEHGLQHKIEEVFEEIQRSNMSKLGEDGR
                     PIYREDGKVMKGPNYFKPDFEKIFK"
     gene            complement(2856..3923)
                     /locus_tag="P5335_08890"
     CDS             complement(2856..3923)
                     /locus_tag="P5335_08890"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRZ75033.1"
                     /translation="MSSKLTNEIDIVKASNSKINSVDFENLTFGNIFTDHMLICDFKE
                     GKWQKPIIKQYEPFLIDPSAKVFHYGQAIFEGMKAYKDENDDVWMFRPDQNYERFNHS
                     AARLAMPEVTEDIFIGGLHALLNLERDWVKKGKGNTLYIRPFMIAIGTGVIAQPSTQY
                     RFMIILSPARAYYSGEVKVLIAEHYSRAANGGIGAAKAAGNYSAQFYPTKLANEKGFQ
                     QIIWTDDATHTKLEEAGTMNVFFRINDTLYTAPTSERILDGVTRKSLIDLAKREGINV
                     EVRSVLVSELIDAAKDGSLKEIFGAGTAAVVNPIVGFSFQETYYELPKIENSYAIQLK
                     EKLTNIQYKLAEDTFGWTVKV"
     gene            4225..4719
                     /locus_tag="P5335_08895"
     CDS             4225..4719
                     /locus_tag="P5335_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HRZ75034.1"
                     /translation="MKKVFVLALVVFSMIACKKTEIKEEAIVAPEKFQSFGDSITAEG
                     AITKEELLAKFETLKEGDTIELKFRSEIKDVCQKKGCWMNMNLSDDKNTFVRFKDYGF
                     FMPFNAAGSEAIVNGKAFISVESVDELKHYAKDAGKSQAAIDSITEPKVSYSFLSNGV
                     LIKE"
     gene            4719..5171
                     /locus_tag="P5335_08900"
     CDS             4719..5171
                     /locus_tag="P5335_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75035.1"
                     /translation="MKNIVVLIFALFTVLSCQKKDTAENCEKPTSKEKSEKKTFEMYK
                     MSEMALLMEQMYVDNQRLKARIEKGEAVGEFPSHFANIHNSIMTDPTENDDFFKKQAK
                     VFLDAQELIYKDPKNAKTHYSNAIQACISCHEVKCSGPIVRIKKLQLN"
     gene            5185..>5609
                     /locus_tag="P5335_08905"
     CDS             5185..>5609
                     /locus_tag="P5335_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRZ75036.1"
                     /translation="MKREIIVTSDGSTSIHLPDWEESYHSKHGAIQEAKHVFIKNGLS
                     FFKGQSVSILEIGFGTGLNAFITFLESQLLNQMVDYVGVEGFPISSQEVLQMNYVNQL
                     NATDFKAVFELFHLCKWEENVTISNHFRLTKRNQLFNEI"
     assembly_gap    5610..5709
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5710..6177
                     /locus_tag="P5335_08910"
     CDS             <5710..6177
                     /locus_tag="P5335_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HRZ75037.1"
                     /translation="KFICQKDAFLCANQGVKIGIEFSRKLGRGLFGGEGFIMQRLEGS
                     GLAFIHAGGTMAKIELQPGEMIKVDTGCVVGFTQTVDYDVQLVGGIRNTIFGGEGLFF
                     ATLKGPGTVYVQSLPFSRLAGRIWAAAPQGGGKHKGEGSLLGGLGNLLDGDNR"
     gene            6280..6477
                     /locus_tag="P5335_08915"
     CDS             6280..6477
                     /locus_tag="P5335_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75038.1"
                     /translation="MTRPMFAYTKKILESVSFDPKLFCKELEKAIRQLLPYEIDQLRD
                     WLLNFTKEKPELKSCLIYVNQ"
     gene            complement(6508..7452)
                     /locus_tag="P5335_08920"
     CDS             complement(6508..7452)
                     /locus_tag="P5335_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRZ75039.1"
                     /translation="MTITQLKYVLAVAEHKNFTLAAEKCFVTQPTLSMQIQKIEEELG
                     ILIFDRSKKPIQLTEIGQKIVNQSKNIVNEAERIQDIVDQQKGFIGGEFRLGIIPTVM
                     PTLLPMFLNNFIKKYPKVNLIIEELNTTEIIVKLKNGHLDAAIAVTPLEEEKIKEVVL
                     YFEPFVAYIPESHAYFEKKEIEISDLNLDEILLLQDGHCFRDGIVNLCKNNNTTSDKF
                     NFHIESGSFETLIKLADEGLGTTLLPYLHTLDLKENDKKKLRHFKNPKPAREVSLIYP
                     KNELKIHIIDALRNVISGVIRGAIAFQDVQIISPIQKK"
BASE COUNT         2441 a         1091 c         1269 g         2609 t
ORIGIN      
        1 attattctat tgcttccgga atatccattt tcggttttgg tagagtgggt tctggagcgg
       61 gttttgaaaa cggtttggct ttaacaggga tgtagatttc agtaatccat tgtgaaggat
      121 attttatatc ttttctactt ttagtgtaaa cttcttgata ggtgccagtt gcactttctt
      181 ccaggttatt ctgtttgatg tattcagttc ctttgtccca agcttcttta ctgtgtgaat
      241 aatctccttt taaggttatt tttactgctt ggtagggaag aatactatct gtgataaatt
      301 cactttccgg agtggtgaat atttcttctt ttaatggaat tgctattgca aattttacaa
      361 gattgttcgg tatatcatac gagtcgaaag ctacaaatgg actaccgaag gttgcgacat
      421 tgttgtcttt cacaaaattc aaaagtagcg gcatttgctt ttggacttta gtatagaaat
      481 cgggtatttt agaaacagag cgtcgtttaa ggtatgtaat tttatttact tcgactagac
      541 catttatgga gatatcgtag gtgtttattt cttttgttag tactttatca agtttggcta
      601 aactattttc aaagttttga gttagttttt tttcaattcc tccagaaaaa agagcataaa
      661 atttccctaa gaaactctga ttacctttgc aactccatcg tacttttgtt ccccctatgg
      721 tgtcacgaaa gattatattt gcttttgatt ctgtaccatt ccatgatata ttttgggcaa
      781 tgctgtcgtt ttcttttaag tagattgttt gcatcttacc gctttctaaa ccaccatccc
      841 aactgaaagc tccaccaaca ccaaccgtgt tttcgggaaa agtaaatttc aattgagtat
      901 catcttcttt caaggaacaa aaatcttccc agtttttata gtcatttaca tactgaaaca
      961 aagtggattt ttccaactta atcactctac tgctggtaac actatagctg ccatctttag
     1021 tggctatata tacgactaat gccaaagcaa ctagaccgat taaaagtaag atatatttca
     1081 atattctcat gatgctctat tatgaatatt caaatgtaaa ggtaggaaat ttattttggt
     1141 ttaaaaatgt agattttttt aatttataaa ttaagcagag ctattaaaaa tcactatttg
     1201 tagggatttt tattttagtt caattcttta aatttacaat gttaattaga aagtttagtt
     1261 tagtttgtaa ccgtgcaatg gtttaaattc cccaaaagaa agtcccgttt taaacgggac
     1321 ttacttattt tagttatttt gtagattaaa aatcatattt tctttccact gcaaaacgga
     1381 ttaaatctgt tttgccatgg atattgattt ttttcatgat gttttttcgg tgagaatcca
     1441 ccgttgtttt gctgatgaat aaagaagtag caatttcatt tgacgttttg ccttcgccga
     1501 tgagttgcag gatttctttt tcacggttgc ttaaaataat ttcttctttg gtaattgaat
     1561 catgtgtaat gatgtcattg tcaaaatagg tttttccact agcaacggtt cgaattgctt
     1621 ctaatacttt ttttaaagag gagtttttta atatatatcc tttagcaccg gcagctttca
     1681 tttgttgaac cgcttcatac tgttcaaaca tactaaagcc aataattcgg gtattgggat
     1741 aaagagccat gattgctttt gtagcacaga ggccgtcaca ttttggcatt cgaatatcag
     1801 ttaaaacaag atccggttgt tttgatttta caatttcaag tagctcttca ccatcattgg
     1861 cttctcctac tacttcaatg tccgtttcaa actcgagcat caatttgatg ccgtctaata
     1921 aggcttggtg gtcttcagca atggctattc gaatcataat ggtatattga ttataacggt
     1981 tgttcctttg ttaataatag aatctattgt aaaagttcat tttgaatgtt taacaatcta
     2041 ttgaatactt ttgattctat tccacctcac ggcattataa tataattgtc taaaccagtt
     2101 ttgttatttc attgctaaag ttaagttatt tattttttaa ataaattttt atgatgaata
     2161 ataatataat aaagagaata aaaccaaaca atacttttag gcttcctttg taatgaattt
     2221 tatgtaaagc aaagtctttc cgataaacat aaatcataat ggctataaaa cagacgacaa
     2281 aaatccctgc aaaaattaat tgacctttgg aaaacatagt tttatttttc aacaaaaata
     2341 gttaattgtt tttgaattga agtgacaaaa gatgttttcc aaaattctat ttttactttt
     2401 taaatttata gaattatgca aaaacaaatt catgccgtaa aagagtttca tgaagctttt
     2461 gaaattggtt ttaaagaatc acctgctgtt catttgggtg aaaataaaaa tctacttcgt
     2521 tataatttaa tgaaagaaga aaatgaagaa taccttgaag ctgccatgaa caatgacttg
     2581 gttgaagtgg ccgatgcgtt aggtgacatg ctttatattt tgtgtggtac aattatagaa
     2641 cacggattgc aacacaaaat tgaagaagtg tttgaagaaa ttcaacgcag caacatgagt
     2701 aagttgggag aggacggaag accaatttat cgcgaagacg gaaaagtgat gaaagggcca
     2761 aattatttca aacctgattt tgaaaagatt tttaaataaa aaaagtgttc agtgaatttg
     2821 gtttcaactg aacactttct ataatagaag ttaacttata ctttaacggt ccatccaaaa
     2881 gtatcttctg ctaatttata ttgaatatta gtcagttttt cttttaattg aatggcatat
     2941 gaattttcaa tttttggtaa ttcataataa gtttcctgaa atgaaaaacc aacaattgga
     3001 ttgacaacgg ccgcggttcc tgccccaaaa atttctttta gactaccatc tttagccgcg
     3061 tcaattaatt ctgaaactaa tacagagcga acttcaacat taattccttc tcgtttcgct
     3121 aaatcaatta aactttttcg agtgacaccg tctaaaatac gttcgctggt tggtgctgta
     3181 tataaggtat cgtttattct aaaaaatacg ttcattgtac cagcttcttc caatttggta
     3241 tgcgttgcat catctgtcca aatgatttgt tggaagcctt tttcattggc taatttagtt
     3301 ggataaaatt gagcagaata gtttccggct gcttttgcag caccaattcc tccattggcc
     3361 gcacggctgt aatgttcagc aataagcact tttacttcgc ctgaataata agcacgggca
     3421 ggagaaagga taatcataaa tctatattga gtagaaggtt gagcaataac acctgttcca
     3481 atcgcaatca taaaagggcg aatatacaac gtgtttcctt tccctttttt tacccaatct
     3541 ctttctaaat ttaagagagc atgcaaacca ccaataaaaa tatcttcggt tacttccggc
     3601 atagctaaac gagcagcaga atgattaaaa cgctcataat tttgatcggg tctaaacatc
     3661 caaacatcat cattttcatc tttatatgct ttcattcctt caaaaatggc ttggccataa
     3721 tgaaaaactt ttgcagaagg gtcaatcagg aatggttcgt attgtttaat gataggtttt
     3781 tgccattttc cttctttaaa atcacagatt aacatgtggt cagtaaaaat atttccgaag
     3841 gtgaggtttt caaaatctac cgagttaatt tttgaattgg aagccttaac tatatcgatt
     3901 tcgttagtaa gtttagaact cattgatttt tatttaaaag tgtatagttg tgtttaagtt
     3961 gttgaaaaac aaagagataa gcatttaaat gcaaaacaat aatgactttg tttaagattt
     4021 tgctaaagta gaaaaaaata gtgtagtttt ttatggtttt gaaaaaatat gacaaattta
     4081 aagcactttt ttatttttcc ttaaactgaa tataaattgt tagagatttc acagcattga
     4141 ttgaaatttt attagaatta caatctaaaa aggattaaac tctttacttt tgtataaata
     4201 atgtaaaatc taattaatta aaacatgaaa aaagtatttg ttctagcatt agtagttttc
     4261 tcaatgatag cttgtaaaaa aacagaaata aaagaagaag caattgtggc tcctgaaaaa
     4321 tttcaatcat ttggtgattc aattaccgct gaaggagcta tcacaaaaga ggaattgttg
     4381 gccaaatttg aaaccttaaa agaaggtgat acaatagaac ttaaattccg ttcagaaatt
     4441 aaagatgttt gtcagaaaaa aggctgttgg atgaatatga atttgagtga tgacaaaaac
     4501 acatttgtac gttttaaaga ttacggattt tttatgccgt ttaatgctgc cggtagcgag
     4561 gcaattgtga atggaaaagc gtttatatca gtagaatcgg ttgatgaatt aaaacattat
     4621 gctaaggatg cgggtaaatc gcaagctgca atagattcaa tcactgaacc aaaagtatct
     4681 tattcgtttt tatcgaatgg tgttctaatt aaagaataat gaaaaacata gtagtattga
     4741 ttttcgctct attcactgtt ttgagttgtc agaaaaagga caccgcagaa aactgtgaaa
     4801 aaccaacttc aaaagagaaa tctgagaaga agacttttga aatgtataaa atgtcagaaa
     4861 tggctttgtt gatggaacaa atgtatgtgg ataatcagcg attaaaagcc cgcattgaaa
     4921 aaggagaagc agtgggtgaa tttccgagtc attttgcgaa tattcataac tcaatcatga
     4981 cggatccaac tgaaaatgac gatttcttta aaaaacaagc aaaagttttt ttagatgctc
     5041 aagagttaat ttataaagat cctaaaaatg ctaaaacaca ttatagtaat gcaattcaag
     5101 cttgtataag ttgtcatgaa gtgaaatgca gtggtccgat tgttcggata aaaaaattac
     5161 aattaaacta gtaaaaaagt tcatttgaaa agagaaataa ttgtaacttc tgatggatca
     5221 acatccattc atctgcctga ttgggaagaa agttatcatt ccaaacacgg agccatacaa
     5281 gaagctaaac atgtattcat caaaaacgga ttgtcatttt tcaaagggca atccgtttcg
     5341 attttagaaa ttggttttgg caccggatta aatgcattta tcactttttt ggaaagtcaa
     5401 ctattgaatc aaatggttga ctatgttggt gttgaaggct ttccgatttc ttctcaggaa
     5461 gttttacaaa tgaattatgt aaatcaatta aatgctactg attttaaagc tgtatttgaa
     5521 ttgtttcatc tttgtaaatg ggaagaaaat gtaacaattt caaatcactt ccgactaaca
     5581 aaacgtaacc aattatttaa tgaaattgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnna aattcatttg ccaaaaagac gcttttttat gtgcgaatca aggagtaaaa
     5761 attggtattg agttttccag aaaattaggg cgtggacttt ttggcggaga aggttttatc
     5821 atgcaacgat tagaaggcag tggattagca tttattcatg cgggaggaac catggcaaaa
     5881 attgaattgc aaccgggaga aatgattaaa gtcgatacag gttgtgtggt aggatttact
     5941 caaactgttg attatgatgt gcaacttgta ggaggaatac gcaatactat ttttggaggg
     6001 gaagggttgt tttttgctac tttaaaaggt ccaggaacag tatatgttca atcgcttcca
     6061 ttcagtcgtc tagcaggcag aatatgggct gccgcccctc aaggaggtgg aaaacacaaa
     6121 ggtgaaggaa gtttattagg gggattagga aatcttttag acggtgataa tcgttaaatt
     6181 ttatataata agagtgttaa ataatgatag tgtattttaa ttttttttat ttttactcaa
     6241 cgttcattta atccaattct actaaaaaaa aactaaagta tgacaagacc aatgtttgct
     6301 tacactaaga aaatcttaga aagcgtgagt tttgatccga aactgttttg taaagagcta
     6361 gaaaaagcaa taagacaatt attgccttat gaaattgatc aactgagaga ttggttgtta
     6421 aactttacaa aggagaagcc tgaattgaaa agttgtttaa tttatgtgaa tcaataaaac
     6481 taaaaggagc ctaaatagct ccttttttta tttcttttga atcggactga tgatttgaac
     6541 atcttgaaag gcaatagctc cacgaataac tccagaaatc acatttcgta aggcatcaat
     6601 gatatgaatc tttaattcat ttttgggata aattaaactg acttcacgtg ccggttttgg
     6661 gtttttaaaa tgacgaagtt tctttttgtc attttctttc aaatctaatg tatgtaaata
     6721 gggtagaaga gttgtgccta aaccttcatc cgctaatttt attaaggttt caaaactgcc
     6781 actttctatg tgaaagttaa atttatccga agtggtgtta ttatttttac acaaatttac
     6841 aattccgtct ctaaaacaat gtccatcttg taataataaa atttcatcta agtttaaatc
     6901 ggaaatttca atttcttttt tttcaaaata agcatgactt tcaggaatat aggcaacaaa
     6961 aggctcaaaa tataaaacaa cttccttaat tttttcttct tccaatggtg taactgcaat
     7021 ggcagcatct aaatgaccat tcttaagttt tactattatt tcagtggtat ttaactcttc
     7081 aataattaaa tttacttttg gatatttttt aataaagtta ttcaaaaaca tcggaagtaa
     7141 ggttggcata actgttggaa ttattcctaa tcgaaattct cctccaataa atcctttctg
     7201 ttgatcaaca atatcttgaa ttcgttccgc ttcgttgaca atattttttg attgattaac
     7261 aattttttgc ccaatttcgg ttaattggat gggtttttta cttcggtcaa aaattaaaat
     7321 gcctaattct tcttcaattt tttgtatttg catacttaaa gtaggctgtg tgacaaaaca
     7381 cttttcggcg gctaatgtaa aatttttatg ttcagcaact gctaaaacat attttaattg
     7441 ggtaatggtc atcaaatagt aatttatgat gcaaatataa aaactatcaa ttttatttat
     7501 atgatttatg
//
LOCUS       DAOTCW010000111         7425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_7127_length_7425_cov_46.008412, whole genome shotgun sequence.
ACCESSION   DAOTCW010000111 DAOTCW010000000
VERSION     DAOTCW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7127_length_7425_cov_46.008412"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..103)
                     /locus_tag="P5335_08925"
     CDS             complement(<1..103)
                     /locus_tag="P5335_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HRZ75040.1"
                     /translation="MKFTETKLQGCFILEPNVIRDERGYFMESFNERT"
     gene            complement(164..1021)
                     /gene="rfbA"
                     /locus_tag="P5335_08930"
     CDS             complement(164..1021)
                     /gene="rfbA"
                     /locus_tag="P5335_08930"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRZ75041.1"
                     /translation="MKGIILAGGSGTRLHPLTLAVSKQLMPIYDKPMIYYPLSTLMWA
                     GINEILIISTPHDLPLFRQLLGDGSKLGCRFEYAVQEHPNGLAEAFIIGKEFVGNDKV
                     ALILGDNIFYGTGLAELLQKNNDPKGGIIYAYHVLDPERYGVVDFDSNGKVLSIEEKP
                     EKPKSNYAVPGIYFYDNSVLEIAANIKPSHRGELEITDVNKEYLNRGNLQVSILDRGT
                     AWLDTGTFQSLMQAGQFVQVIEERQGLKIGAIEEAAYRMGFIDANQLKKIAEPLLKSG
                     YGTHLMSLI"
     gene            complement(1041..2006)
                     /locus_tag="P5335_08935"
     CDS             complement(1041..2006)
                     /locus_tag="P5335_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75042.1"
                     /translation="MSVNSSDNQEIDLGQVSKKIGQTYQNFLSWVFRGILFVKRNFIL
                     LLILFIVGAALGFYLDKKVASYNHQIIISPNFGSTDYVYAKIDLLQSKINENDTTFLK
                     EIGIKDTKKFGKISVMPIIDVYKFISNKAENFELIKLLAEDGNIDKIVENKLTSKNYP
                     FHVVSFTTSKISSEEKTVNPILNYLNDSQYYKALQKEYLNNIQLKMRANDSIINQIDA
                     LMNQFKKTASGSSQSDKLVYYNENTQLNEVIKTKDNLVREQGLLRIDLISLDKVVKDN
                     SVILNSKDTKGTNGKMKFLIPFLFLILFMTVKGFIRFYNNQLAKN"
     gene            complement(2012..>3030)
                     /locus_tag="P5335_08940"
     CDS             complement(2012..>3030)
                     /locus_tag="P5335_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75043.1"
                     /translation="LAIEEIVENNKRTITFSPILRERLGHHIHGEIWATQIKDVLKKN
                     DLLGRPIHIISANMHSVMNSIFATNVLKTKFKDKTDFFIYEELSKSGANDLRNKVEEV
                     ALKSGMMSVLDESGTNIDVQIFDTAKIDFSKTSFSKAKVGEQKPVLIVMDYAFGEQAY
                     ETIDELLKPYKEGKSKVTFLNVESVSIMGKAGILQGGKGDIMIPSAHINEGTGDNYPF
                     ENELTVSMFNGNDIPVFAGPMVTVLGTSLQNKDLLKFFHESTWGVIGLEMEGAYYQKA
                     IQSASKIRKSIPEEVKVRYAYYASDNPLETGSTLASGGLGTTGVKPTYLITIKILEQI
                     FNSK"
     assembly_gap    3031..3130
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3131..3861)
                     /locus_tag="P5335_08945"
     CDS             complement(<3131..3861)
                     /locus_tag="P5335_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75044.1"
                     /translation="MKEIKNISRSRAQESSSAIERLYITMRHLFNRGFYKPMGISGET
                     LREALLQLRPEIYGSIAEEKVELNGLLYVIERLPVGIEECRYINLTSDEGYSKSHFTA
                     IVPPKRRRNCYRIDEEQMNVEITRGRSDIYDILTHLTFIFIESHKIKDRVLLDENQYE
                     VTRDWQKVEQYVLQPKKLSQIDKEKAISHASNVLGRSFSEVLAIYDSFGTAEKPDRFL
                     HIIYWLGKLAIEEIVENNKRTITFSP"
     gene            complement(3929..5425)
                     /locus_tag="P5335_08950"
     CDS             complement(3929..5425)
                     /locus_tag="P5335_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRZ75045.1"
                     /translation="MRIKVLAAKLLARKAHKDTQKWASKPEEIQQKVFKSLIKQASGT
                     LFGNDHHFTAIKTHNDFVKYVPVRDYEALKSYVDLVVEGKENILWKGKPVYFAKTSGT
                     TSGAKYIPLTKESIPFHIQAARNAILSYIHETGKADFVKGKMIFLQGSPILEEKNGIK
                     LGRLSGIVAHYVPKYLLKNRMPSWETNCIEDWETKVNAIVEETFNENMTVISGIPAWV
                     QMYFEKLQQKGNKKVGEIFNNFNLFIYGGVNFEPYRAKFENLIGRKVDSIELFPASEG
                     FFAYQDSQKEIGMLLLLNSGIFYEFIKADDFFTENPKRHTIGEVELGVNYALIISTNA
                     GLWAYNIGDTVQFTSLKPYRVIVSGRIKHFISAFGEHVIGKEVECALKEAMEGTTIRV
                     NEFTVAPQISPKEGLPFHEWLIEFENEPDDMDAFALKIDIAMRKQNVYYDDLIVGNVL
                     RTLVVTKVNKNGFQDYMKSIGKLGGQNKLPRLSNDRKIADVLNQNKKT"
     gene            complement(5428..6297)
                     /locus_tag="P5335_08955"
     CDS             complement(5428..6297)
                     /locus_tag="P5335_08955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRZ75046.1"
                     /translation="MSEKRLKRQQLKKQLFTKNRLVILNEETFEEIFSLKLNLMNVFV
                     VLTISSILLISITTYIIAFTPLREFIPGYASNKLKKDATILAVKSDSLAIEVKKNEMY
                     INSIKKILTGDLEYAKFNKDSILAQENPDNLKADLNPSEDEKKLREQVALEDKYNLFE
                     KAQSKVNLVLFPPVRGHITEKYSSVNKHFAVDIAVAKDTPIKSVANGSVVFAEWTPST
                     GNVIIIKHRDGLLSVYKHAASLTKTQGDVVRTGEVIALAGSTGKESTGIHLHFELWKD
                     GFTIDPSQFIDFE"
     gene            6470..6667
                     /locus_tag="P5335_08960"
     CDS             6470..6667
                     /locus_tag="P5335_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRZ75047.1"
                     /translation="MNALAIFLGMIGPWQIGLIIVVVLLLFGGKKIPELMKGLGSGIK
                     EFKNAAKDDQPAEKKEEEKKE"
     gene            complement(6733..>7425)
                     /locus_tag="P5335_08965"
     CDS             complement(6733..>7425)
                     /locus_tag="P5335_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRZ75048.1"
                     /translation="NSVGVNLNTASKSLLSYVSGIGEKMAENIIAFRQENGPFEDRKQ
                     LKKVPRLGEKAYQQSAAFIRIQNGKNPLDNSAVHPEAYSIVEKMAKDLKISLTDLIGN
                     KEKISLIKPENYTNQTIGILGIKDILKELEKPGLDPRKSAKVFEFDPNVKKMSDLRTG
                     MILPGIVNNITAFGCFVDIGLKESGLVHISQLKAGFVSDVNEVVKLHQHVQVKVVEVD
                     EVRKRIQLTMVI"
BASE COUNT         2251 a         1347 c         1042 g         2685 t
ORIGIN      
        1 aagttcgttc gttaaaactt tccataaaat agcctctttc atcacgaatg acatttggtt
       61 ctaaaataaa acaaccttga agtttggttt ctgtaaattt catttttcaa tttcaacgac
      121 aatttcaaag ttcaaaaaca attagcttga aacttgaatc aatttatatt aagctcatca
      181 aatgcgttcc gtaaccactt tttaaaagtg gttcagctat tttttttaat tgattggcat
      241 cgataaaacc cattctgtaa gcagcttctt caattgcacc aatttttaaa ccttggcgtt
      301 cttcaattac ttgaacaaac tgtccggctt gcataagcga ttgaaacgtg ccggtgtcaa
      361 gccatgcagt tcctctgtcg agtatgctca cttgaagatt tcctcgattt aaatattctt
      421 tgtttacatc ggttatttcc aattcacctc gatgactagg ttttatattt gctgctattt
      481 ccaacacaga attgtcatag aaataaattc cgggaactgc ataatttgat ttaggttttt
      541 ccggtttttc ttcaattgac aatacttttc cattagaatc aaaatcgaca acaccataac
      601 gttccggatc caaaacgtgg taggcataaa taattccgcc tttcggatca ttgttttttt
      661 gtagcaattc ggctaaaccg gttccataaa aaatattatc accaagaatt aatgcgactt
      721 tatcattgcc cacaaattct tttccaataa taaatgcttc tgctaaacca tttggatgtt
      781 cttgaacagc atattcaaaa cgacagccca atttgcttcc atctcccaaa agttgtcgaa
      841 ataaaggcaa atcatgcggt gtagatataa ttaaaatttc atttattccg gcccacatta
      901 aggttgacaa cggataataa atcattggtt tgtcgtaaat aggcattaat tgtttactga
      961 ccgctaatgt taacggatgt aatctcgttc cagagcctcc ggctaatata attcctttca
     1021 tgaaatttgt attaattcct ttaattctta gccaattgat tgttataaaa tcttataaat
     1081 cctttgactg tcatgaataa aatcaaaaac aaaaatggaa tcaaaaactt catttttcca
     1141 tttgtccctt tggtatcttt actatttaga attacactat tgtcttttac aactttatct
     1201 aaactgatta aatcaattcg taacaaacct tgttctctca ctaaattgtc tttcgtttta
     1261 atgacttcat taagttgtgt attctcatta taataaacca atttatcgct ttgtgaagac
     1321 ccgctagctg tctttttaaa ctgattcatt aaagcgtcta tttgattaat tattgaatca
     1381 ttagctctca ttttaagctg aatattattc aaatattctt tttgtaatgc tttataatat
     1441 tgactgtcat ttaaataatt aagaatcgga ttaactgttt tttcttcaga agaaattttt
     1501 gaagtagtaa acgaaaccac atgaaaggga taattcttac tggttaattt attttctact
     1561 attttatcaa tattgccgtc ttctgctaat agtttgatta attcaaaatt ttccgcttta
     1621 ttgcttataa atttgtaaac atcaattatt ggcataacag aaatttttcc gaactttttt
     1681 gtgtctttta ttccaatttc ttttaaaaaa gtggtgtcat tttcattgat ttttgattgt
     1741 aacaaatcta ttttagcata cacataatct gtactaccaa aattgggcga gataataatt
     1801 tgatgattgt aacttgctac ttttttatct aaataaaaac ctaatgcagc tccgacaata
     1861 aatagaatta gcaacaaaat gaaattcctt tttacaaaaa gaattcctct aaaaacccag
     1921 cttaaaaaat tttgataggt ttgtcctatt tttttagata cttgtcctaa atcaatttcc
     1981 tggttatctg atgagtttac gctcataatt tttattttga attaaaaatt tgttctagta
     2041 ttttaattgt aattaaatag gttggtttta caccggttgt gcctaatcct ccggatgcta
     2101 atgtgcttcc agtttccaat ggattatctg aagcatagta cgcataacgc acttttactt
     2161 cttctggtat gctttttctg attttggagg ctgattgaat tgctttttga taataggctc
     2221 cttccatttc aagaccgata acaccccatg ttgattcatg gaaaaatttt aataaatctt
     2281 tgttctgtaa cgatgttcct aatacggtca ccattggtcc cgcaaaaaca ggaatatcat
     2341 ttccattaaa catagaaacg gtcaattcgt tttcaaaagg ataattatca cccgtacctt
     2401 cgttgatatg tgccgatgga atcataatat caccttttcc tccttgtaaa attccggctt
     2461 tacccatgat ggaaaccgat tctacattta aaaaggttac tttgcttttt ccttctttgt
     2521 atggttttaa tagttcgtca atggtttcat aggcttgttc gccaaaagca taatccatca
     2581 caattaaaac cggtttttgc tctcctactt tagcttttga aaaagatgtt tttgaaaaat
     2641 ctattttagc tgtatcaaaa atttgcacat ctatatttgt tccggattcg tctaaaactg
     2701 acatcattcc tgattttaaa gccacttctt caactttatt tcgcaaatca ttggctccgg
     2761 atttacttaa ttcttcataa ataaagaaat ccgttttatc tttgaacttt gtttttaaca
     2821 cattcgttgc aaaaatggaa ttcatcacac tatgcatatt ggcactaatg atatgaatag
     2881 gccttcctaa taaatcattt ttctttaaaa catccttaat ttgagtggcc caaatttctc
     2941 catgaatatg atgtcctaat cgttctctta aaattggact gaatgtaatc gttcgtttat
     3001 tgttttcaac aatttcttca atagctaatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn ggactgaatg taatcgttcg tttattgttt tcaacaattt cttcaatagc
     3181 taattttcct aaccaataaa taatgtgtaa aaaacgatcc ggtttttctg ctgttccaaa
     3241 cgaatcataa atagctaata cctctgaaaa gcttcttcct aacacattcg atgcgtgaga
     3301 tattgctttt tctttatcaa tttgtgataa ttttttgggt tgcaaaacat actgttctac
     3361 tttttgccaa tctcgtgtta cttcgtattg attttcatct aataaaacac ggtctttaat
     3421 tttatgtgat tctataaaaa taaaggtcaa atgggttaaa atatcataaa tgtctgaacg
     3481 cccacgtgta atttcaacgt tcatctgctc ttcgtcaatt cggtagcaat ttcttcttcg
     3541 tttaggagga acaattgcag taaaatgtga ttttgaatag ccttcgtctg atgttaaatt
     3601 aatataccga cattcttcta tgccaaccgg aagccgttcg ataacataca ataatccgtt
     3661 gagttctact ttttcttcag caatggatcc ataaatttca ggacgcaatt gtaataacgc
     3721 ttcacgtaaa gtttctcctg aaattcccat cggtttataa aagccgcgat taaacaagtg
     3781 acgcatcgtg atatataatc gttcaatagc tgaagatgat tcttgagctc gggaacggga
     3841 aatatttttt atttctttca tttataaggt attaagtatt tcaaattgcc aataaaaatc
     3901 aatttgtata cttttatttt ttgcaaattt aggttttttt attttggtta agtacatctg
     3961 ctatttttct atcattactt agtcttggaa gcttattttg acctcctaat ttaccaatgg
     4021 atttcatata atcctgaaat ccgtttttgt tcacttttgt aacaactaag gtacgtaaaa
     4081 cgttacccac aatcaaatcg tcataataca cattttgttt ccgcatggcg atatctattt
     4141 tcaaagcaaa agcatccata tcatccggct cattttcaaa ttcaattaac cattcatgaa
     4201 aaggcaagcc ctctttagga gaaatttgtg gagccaccgt aaattcattt accctaattg
     4261 ttgttccttc catcgcttct tttaaagcac attctacttc tttaccaatt acatgttcgc
     4321 caaaggcaga aataaaatgt ttaattcgtc ccgaaacaat taccctataa ggctttaaac
     4381 tcgtaaactg aactgtatct ccaatattat aagcccaaag acccgcatta gttgaaataa
     4441 ttaaagcata atttacccct aattctactt ctccaatggt atgccttttt ggattttcag
     4501 taaaaaaatc atctgcttta ataaattcat agaaaattcc tgaatttaaa agtaataaca
     4561 tacctatttc cttctgagag tcctggtaag caaaaaatcc ttccgaagca gggaacaact
     4621 caatcgaatc aacttttctg cctattaaat tttcaaactt ggcacgataa ggttcaaaat
     4681 taactccacc gtagataaag agattaaagt tattaaaaat ttctccaact ttcttattcc
     4741 ctttttgttg tagtttttca aaatacatct gcacccaagc cggaatacct gaaatgacag
     4801 tcatgttttc gttaaaagtt tcttctacta tcgcattcac cttggtttcc caatcttcga
     4861 tacaattggt ttcccatgaa ggcattctat ttttcaaaag gtattttggc acataatgag
     4921 cgacaattcc ggaaagtcgt cctaatttta ttccgttttt ttcttctaat attgggctcc
     4981 cttgaaggaa aatcattttc cccttaacaa aatctgcctt gccggtttcg tgaatataac
     5041 tcaaaatagc atttcgggct gcctgaatat gaaacgggat agattctttc gtaagcggaa
     5101 tatattttgc tccggatgtt gtgccggaag ttttagcaaa ataaactggt tttcctttcc
     5161 ataaaatatt ctctttccct tcaactacta aatcaacata cgatttcaat gcttcataat
     5221 ctctcaccgg aacatatttg acaaaatcat tatgcgtttt aatggcagta aaatggtgat
     5281 cattgccaaa caaagttcct gaagcttgtt taatcaaact tttaaaaacc ttttgctgaa
     5341 tctcttccgg ttttgaggcc catttttggg tatccttatg tgcttttctg gctagaagtt
     5401 tagccgctaa aactttaatt cgcataatta ttcaaaatct ataaattgag aggggtcaat
     5461 ggtaaaaccg tctttccata attcaaaatg caagtgaatt ccggttgatt cttttccggt
     5521 tgacccggca agtgcaataa cttctcccgt tctaaccaca tctccttgtg tttttgttaa
     5581 tgatgccgcg tgtttgtaaa cagacagtaa accatctcga tgtttaataa ttatcacatt
     5641 tccggtggag ggtgtccact ctgcaaaaac aacactccca ttggcaactg acttaatggg
     5701 tgtgtcttta gcaaccgcaa tatcaactgc aaaatgctta ttaaccgaac tgtatttttc
     5761 agtaatgtgg cctcttaccg gtggaaataa aactaaattt actttcgatt gagctttttc
     5821 aaaaaggtta tacttgtctt ccaaagcaac ttgctcccgg agttttttct catcctctga
     5881 aggatttaaa tcagctttta aattatccgg attttcttga gctaatatag agtctttatt
     5941 aaacttggca tattctaaat caccggttaa aatcttttta atagaattta tgtacatttc
     6001 atttttcttc acttcaatag ccaaagaatc cgatttaacc gctaaaatag tagcatcctt
     6061 ttttaattta ttggaagcat atcccggaat aaattcacgt aatggtgtaa atgcaattat
     6121 ataagttgta attgaaatta acaaaataga agaaatagta agcacaacaa aaacattcat
     6181 caagttgagc tttaatgaaa agatctcttc gaaggtttcc tcatttaaaa tcaccaacct
     6241 attcttggta aacaattgct tcttgagttg ttgtcttttg agccgttttt cagacataat
     6301 tcaattttaa tatacaaata tagtaattaa actgtcttta aaaagaggtg tttttcttgt
     6361 tttagataaa aaaccactca ctttaactaa cttttaagtt aagaaatact tgaaatcgaa
     6421 acaagtcccg taatttcttt ttacatttgt cctttcaaat atgtaaacta tgaatgcatt
     6481 agctatattt ttaggaatga taggtccttg gcagatagga ttaattatcg ttgttgtttt
     6541 attacttttc ggaggtaaaa aaattccgga attaatgaaa ggtttaggta gcggtattaa
     6601 agaatttaaa aatgctgcta aagatgatca accggcagaa aaaaaagagg aagaaaaaaa
     6661 ggaataactt ttttaatttc ttaccaaaaa atccaaattt catgatactt aaggtgaaat
     6721 ttggattttt ttttaaatca ccattgtcaa ctggattctt ttccttactt catccacctc
     6781 aaccactttt acttgaacat gttgatgtaa tttcaccacc tcgttaacat ccgacacaaa
     6841 ccctgctttg agttgtgaaa tatgtaccaa accactttct tttaatccaa tatccacaaa
     6901 acaaccaaaa gcagtaatgt tgttgacaat tcctggtaaa atcatgccgg ttctcaagtc
     6961 ggacattttt ttgacattcg ggtcaaattc aaaaaccttt gctgattttc ttgggtcaag
     7021 tcccggtttt tccagttctt ttaaaatgtc tttaattcct aaaattccga tggtttggtt
     7081 ggtataattt tcaggtttga ttaaggatat tttctctttg tttccaatta aatcggttag
     7141 tgaaattttt aagtctttcg ccattttttc cacgattgaa taagcttccg gatgtaccgc
     7201 cgaattatcc aatggatttt ttccgttttg aatacgaata aatgcagctg attgttgata
     7261 ggctttttca cccaatctcg gcactttttt aagttgtttt ctgtcttcaa acggaccgtt
     7321 ttcttgtcgg aatgcaataa tattttcggc cattttttca ccaattccag aaacataact
     7381 caacaaggat ttactggctg tattcaaatt tacacccact gaatt
//
LOCUS       DAOTCW010000112         7197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_7482_length_7197_cov_43.893587, whole genome shotgun sequence.
ACCESSION   DAOTCW010000112 DAOTCW010000000
VERSION     DAOTCW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7482_length_7197_cov_43.893587"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="P5335_08970"
     CDS             <1..372
                     /locus_tag="P5335_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRZ75049.1"
                     /translation="DSTKVIKKLYEKADRVLIDAPCSGLGVLKRNPDSKWKLQPEFID
                     NIRKVQAEVLESYSRIVKPGGKMVYATCSILPSENQEQIKRFLTTENGKNFTFVQDKK
                     ILASESGFDGFYMALLERKNE"
     gene            377..1429
                     /locus_tag="P5335_08975"
     CDS             377..1429
                     /locus_tag="P5335_08975"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRZ75050.1"
                     /translation="MKKIYSILLLLVVTLGFSQQAYYNGINFSLNGQPLKNQLAQLII
                     TTHTKELSYSQVWNALKITDLDPSNSANVLLVYGWENGSDGDVTNDLSRNKNDNGGSN
                     GQWNREHVYAQSVGTPDLGQSGPGADAHMLRACDVQRNGTRANRKYATGSGNSTVVGA
                     NWYPGDEWKGDVARIIMYMYLHYGNQCLPSNIGVGANADAGDDMIDLFLTWNAEDPVS
                     QTEINRNNYLTNANNTYGQGNRNPFIDNPFLATKIWGGLPAQDIWGTLSTTQMVAFEF
                     SLYPNPTNDRRINIDSEMEVDEIQLININGQLMQQIQKPTRTNNTYTVENLPSGFYFI
                     KISSENQSITKKVIVN"
     gene            1470..>2249
                     /locus_tag="P5335_08980"
     CDS             1470..>2249
                     /locus_tag="P5335_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRZ75051.1"
                     /translation="MKKIYIVVYLFSSLIFAQNSIETIPPGYYNNAIGFTGYTLKTKL
                     KTIITNGHDGNSYDNLFTDIKGFKTTDVDHYYEDDGSILDMYSEKPNATDAYNYSYYA
                     NDKCGSYQGESDCYNGEHIVPQSTYNSQMPMVGDIHQLIPTDGYVNNRRSNYPFGIID
                     NPTWTSTNGSKLGDISVTEYSGKAFEPIDEFKGDIARILFYFATRYETQVDNYSFPMF
                     NGTENQVFKLPFLQMLVDWHNSDPVSQFEIDRNDNAYIYQGN"
     assembly_gap    2250..2349
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2391..3404
                     /locus_tag="P5335_08985"
     CDS             2391..3404
                     /locus_tag="P5335_08985"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRZ75052.1"
                     /translation="MTEIQIETAKMLLATSKKIVILPHRNPDGDAMGSTLALYHFLKK
                     LNHQPILISPNEFPNFLAWMPGSETVLIYEKDKVGTKAVLEQAELIFTLDFNALHRTG
                     EMEHDLTTLKAPFIMIDHHQAPDSYAEVTFSDTTYGSTCEMVYDFIAVLNGLELIDKT
                     IATCLYTGIVTDSGSFKFPKTTGKTHRTVAHLIDLGIDNSEIHNLLFNNNSFESLQLL
                     GKALQNMKLLKAQKTAYITLTQEELDRYHFQKGDTEGIVNYGLSIKDIIFAAIFIENK
                     EEGIIKISLRSIGDFDVNQLAREHFHGGGHINASGGKSLVSMKETINNFEQIVKNNPK
                     LKF"
     gene            3444..3986
                     /gene="gldI"
                     /locus_tag="P5335_08990"
     CDS             3444..3986
                     /gene="gldI"
                     /locus_tag="P5335_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated peptidyl-prolyl
                     isomerase GldI"
                     /protein_id="HRZ75053.1"
                     /translation="MLFAYGCKEPEARKPIYKSSGSFMKESIIRNKKLIVAEEKAIDS
                     IIKSNPEKKYLTSEKGYWYTYEIQNQQDTLRPKRGDVAFFDYEIFDLKGNVIYTALEL
                     RPQNYLVDKQEIMIGLRDGIKLMRKNEKVSFLFPSHIAYGYLGDKNRIGSNVPIICTV
                     TLTDFKKESVIKNNNNSSNQ"
     gene            4000..5127
                     /locus_tag="P5335_08995"
     CDS             4000..5127
                     /locus_tag="P5335_08995"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRZ75054.1"
                     /translation="MKKIVFLFLSLTSLLLVSCGENYDRLGDGIFGVIKTGKGEIIVS
                     LEYNKTPITVANFILLAEGKNKHVTLESKKGAPFYDGLKFHRVEANFMIQGGDPNGDG
                     TGGPGYNFMDEITDLSHEGPGTLSMANAGPGTNGSQFFITHVETKFLDGRHTVFGKVK
                     EGQEVVNAIVKDDVIESIIIVRNGEAAKKFDAVKVFDDRLKTEAENRKKMEAEEAIKK
                     EAFLAEFKEVIDTKLAFFETQKKAAQKTASGLQYAIYEKGKGKKPANGSTVYVHYAGF
                     FESGELFDSSKEDVSKAFGKFDQNRADAKAYTPFPFQYGNKTGLIPGFLEGLEKLNFG
                     DKAILFIPSQLGYGEAGAGGIIPPNSNLIFEIELFEELPKQ"
     gene            5146..6159
                     /locus_tag="P5335_09000"
     CDS             5146..6159
                     /locus_tag="P5335_09000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRZ75055.1"
                     /translation="MKKIISIFILSISLSTLAQTENGLYAEIQTIKGKILLKLEYEKT
                     PITVANFVSLAEGTNMEVTDANLKGKPFFNGLKFHRVIANFMIQGGDPTGTGSGSPGY
                     KFADEITDLKHNSPGILSMANAGPGTNGSQFFITHVATPHLDGKHTVFGKVIEGQDVV
                     NAIAQNDLMDKVTIIRVGDAAKKFDAPKVFQGRLAAEIEKQKKKEEALKIEKVKFNEY
                     VKKTFPKAKISPSGLAYIIEKTGTGENAKPGNTVSVHYIGELENGKQFDSSYERNQPI
                     EFVLGQGRVIPGWEEGIALLNKGAKVKLIIPYWLAYGESGRPPVIPEKATLIFNTELI
                     EIK"
     gene            complement(6200..6637)
                     /locus_tag="P5335_09005"
     CDS             complement(6200..6637)
                     /locus_tag="P5335_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75056.1"
                     /translation="MELKPKFGLDQLLFGMKQKDVESLFGKADSVFKDEDDNQILLYN
                     AFKARLTFYQEEDFKLGYIIISNPEIRLFNQKVIGQDLIEVKSILEKKGFTLFEEEVF
                     DSVTNYFNEENWIILQAEFGQVIKVELGAIIKNQDEFDWKFKT"
     gene            complement(6640..>7197)
                     /locus_tag="P5335_09010"
     CDS             complement(6640..>7197)
                     /locus_tag="P5335_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRZ75057.1"
                     /translation="ANQIALSLASLTFMFAMGLSVTAMIRVGNQKGLQDYKKLRIVAV
                     SIFLLATLLEIVFALLFIIFHKVLPIPFLNLEQTIHLQDNLEVVAIASQLLIVAALFQ
                     ISDGLQVVVLGALRGLQDVKIPMYITFVAYWVIGFPISIYLGLYTELKAIGVWIGLLA
                     GLSTAAVFLFYRFNTLTKKLIIQNK"
BASE COUNT         2592 a         1152 c         1185 g         2168 t
ORIGIN      
        1 gattctacga aagtaattaa aaaactatac gaaaaagctg atcgtgtatt aatcgatgct
       61 ccttgtagtg gcctaggtgt tttaaaaaga aatcccgata gcaaatggaa actacaaccg
      121 gaatttatag acaatatccg taaagtgcaa gcggaagttt tagaaagtta ttccagaatt
      181 gtaaaaccgg gtggaaaaat ggtttatgct acctgttcta tcctaccttc tgaaaaccaa
      241 gaacaaatta agcgtttctt aactactgaa aacggtaaaa actttacctt tgtacaagat
      301 aaaaaaattc tagcctcaga atctggtttt gatgggtttt atatggcctt attagaacgt
      361 aaaaacgaat aattaaatga aaaaaatcta ctctatttta ttgttattag ttgtaacact
      421 tgggttttct cagcaagcat attacaatgg aattaacttt agtttaaacg gtcaaccgct
      481 taaaaatcaa ttggctcaac taataattac tactcatacc aaagagctta gttattctca
      541 agtttggaat gctttaaaaa ttactgattt agatccttct aattctgcaa atgtattgtt
      601 agtatacggt tgggaaaacg gttccgatgg agatgtaacc aacgacttaa gcagaaataa
      661 aaatgataat ggcggtagta atggtcagtg gaacagagag cacgtttatg cccaatctgt
      721 tggaacacct gacttaggtc aaagtggtcc tggagctgac gctcatatgc tacgtgcttg
      781 tgacgttcag cgtaatggca caagagcaaa tcgaaaatat gctaccggtt ctggaaattc
      841 tactgttgtc ggtgcaaatt ggtatcccgg tgatgaatgg aaaggtgatg tagcccgaat
      901 cattatgtat atgtatcttc attatgggaa tcaatgctta ccttcaaata ttggagttgg
      961 agccaatgca gatgccggtg atgatatgat tgatttattt ttaacttgga atgctgaaga
     1021 tccggtttca caaactgaaa taaatagaaa taactacttg actaatgcaa ataacacata
     1081 cggtcaagga aacagaaatc catttattga taatcctttt ttagctacaa aaatttgggg
     1141 aggtcttcct gctcaagata tttggggaac tttatcaact acacaaatgg ttgcttttga
     1201 gtttagcctt tatccaaatc caaccaatga cagaagaatt aatatagatt ctgaaatgga
     1261 agtggacgaa attcaattaa tcaatatcaa tggtcaattg atgcaacaaa ttcaaaaacc
     1321 aactcgaact aataacacct atacggttga aaatttacct tccggatttt attttataaa
     1381 aatatcttct gaaaatcaat ctattactaa aaaagtgatt gtaaattagt tactttattt
     1441 aaaaaacaaa tacctcaaat taaattataa tgaaaaaaat ttacatcgtg gtgtacctat
     1501 tttcatcact tattttcgct caaaattcaa tagaaacaat tcccccggga tattataata
     1561 atgcgattgg ttttacaggt tatactttaa aaacaaaatt gaaaacaata attactaatg
     1621 gacatgatgg taattcgtat gacaatcttt tcacggatat aaaaggattt aaaactaccg
     1681 atgtagatca ttattatgaa gatgatggtt ctattttaga tatgtattct gaaaaaccca
     1741 atgcaacaga tgcctacaac tatagttatt atgccaacga caaatgtggt agttaccaag
     1801 gtgaatcaga ttgttataac ggtgaacata tagttcctca aagcacttat aactcacaaa
     1861 tgccgatggt tggcgatatc catcaactta ttccaacaga tggttatgta aacaatcgaa
     1921 gaagcaatta tccttttggt attattgata atccaacttg gacttcaaca aatggttcca
     1981 aacttggtga tatatcggtt accgaatatt ccggaaaagc gttcgaacct atcgatgaat
     2041 ttaaaggaga tattgcccgg atattattct attttgccac tcgctatgaa acgcaagtag
     2101 acaattattc ctttccaatg tttaatggaa ctgaaaatca agtattcaaa ttaccttttt
     2161 tacaaatgct agtagattgg cataattcag accctgtaag ccagtttgaa attgaccgta
     2221 atgataatgc ttatatttat caaggaaacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnna aatgagaacc ttttgctaaa aaaatgtaac ttcgcaaact atgacagaaa
     2401 ttcaaattga aacagcaaaa atgctattgg cgacttctaa aaaaatcgtc attttaccac
     2461 atagaaatcc agatggtgat gccatgggtt caaccttagc tttataccat tttctaaaaa
     2521 aactaaatca ccaacctata cttatttccc ctaatgaatt tcctaatttt ttagcttgga
     2581 tgccgggttc agaaaccgtt ctgatatatg aaaaagataa agtaggtaca aaagcagttt
     2641 tagaacaagc agaattgatt tttactttag attttaatgc tcttcataga actggagaaa
     2701 tggaacatga tttgactaca ctcaaagctc catttatcat gatagatcat caccaagccc
     2761 ccgattcata tgcggaagta accttttctg acacaactta tggttcaacc tgtgaaatgg
     2821 tttatgattt tattgcagtt ttaaatggtt tagaattaat agataaaacc atcgcaactt
     2881 gtttatatac aggaattgta actgattccg gttcttttaa attcccaaaa acaacaggaa
     2941 agactcaccg aacggttgct catttaattg atttaggaat tgataatagc gaaattcata
     3001 atttgttgtt caacaataat tcattcgaaa gcttacaatt actaggaaaa gctcttcaaa
     3061 acatgaaact tttgaaagct caaaaaacag catatattac tttaacacaa gaagaacttg
     3121 atcgctatca ctttcaaaaa ggagatacgg aaggaattgt aaattatgga ttatcaatca
     3181 aagacattat ctttgctgcc atttttatcg aaaacaaaga agaaggaatt attaaaattt
     3241 cgctccgttc catcggtgat tttgacgtaa atcaactagc tcgggaacat tttcatggtg
     3301 gcggtcacat caatgcatcc ggtggaaaat cattagtttc gatgaaggaa accattaata
     3361 attttgaaca aattgtaaaa aacaacccta aattaaaatt ctaaatgaaa cttaagtatt
     3421 tttttttcct tttatttttt ggcatgctat tcgcatacgg ttgcaaagaa ccggaagcaa
     3481 gaaagcccat ttataaatcg tccggtagtt ttatgaaaga gtcaattatt agaaataaaa
     3541 aattaattgt agccgaagaa aaagcaattg attccatcat taaaagtaat cctgaaaaaa
     3601 aatatcttac ttctgaaaaa ggatattggt atacgtatga aatacaaaac caacaagata
     3661 ctttgcgccc taaacgtggg gatgttgctt tttttgatta tgagattttt gatttaaaag
     3721 gaaatgttat ttatactgca ttagagctca gacctcaaaa ttatttagtt gacaaacaag
     3781 aaataatgat tggccttcgt gacggtataa aattaatgcg aaaaaatgaa aaagtgtctt
     3841 ttctgtttcc ctctcacatc gcttatggct atttgggtga taaaaacaga attggttcaa
     3901 atgttccgat aatctgtact gttaccttaa cagatttcaa aaaagaaagt gtaataaaga
     3961 ataataacaa cagctcaaat caataattat ttaaaaaaaa tgaaaaaaat tgtatttcta
     4021 tttttaagtt tgacttctct tcttcttgtt tcttgtggag agaactatga ccgtttagga
     4081 gacggtattt ttggtgtcat aaaaaccggt aaaggggaaa taattgtatc cttagaatac
     4141 aacaaaacac ctataaccgt agcaaacttt atattattag ccgaagggaa aaataagcat
     4201 gtaactttag aatcaaaaaa gggagcccct ttttatgatg gtttaaaatt tcatcgcgtg
     4261 gaagctaatt ttatgattca gggtggcgat ccaaatggag acggaaccgg cggaccgggt
     4321 tataatttta tggatgaaat aaccgattta tcacacgaag gtccgggcac tttatcgatg
     4381 gcaaatgcag gtcccggaac gaatggttca caatttttta taacccatgt tgaaacaaag
     4441 tttttagatg gtagacatac tgtttttggt aaagtaaaag aaggtcagga agtagtaaat
     4501 gctattgtaa aagatgatgt aatcgagagt attattatcg ttagaaacgg tgaagcagct
     4561 aaaaagtttg atgctgtgaa agtatttgat gaccgattga aaacagaagc agaaaacaga
     4621 aaaaaaatgg aagcggaaga ggccatcaaa aaagaggcat ttcttgctga attcaaagaa
     4681 gttatagata caaaattagc cttcttcgaa acccagaaaa aagcagctca aaaaacagct
     4741 tccggattac agtatgctat ttatgaaaaa ggaaaaggaa aaaaacctgc aaatggttcc
     4801 actgtttatg ttcactacgc cggatttttt gaaagcggag aactttttga cagtagtaaa
     4861 gaggatgttt caaaagcttt tggaaaattt gatcaaaaca gagccgatgc taaagcctat
     4921 accccatttc catttcaata cggcaataaa actggtttaa ttccgggatt tttagaagga
     4981 ttagaaaaat taaactttgg agataaggct attttattca taccttctca attaggttat
     5041 ggtgaagcag gtgctggcgg aattattccc ccaaattcaa atttaatttt tgaaatcgaa
     5101 ctatttgaag aattacctaa acaataatta taaaattaac aaaacatgaa aaaaataatt
     5161 tcaattttca ttctatccat tagtttgtct acccttgctc aaactgaaaa cggtctatac
     5221 gcagaaattc aaactataaa aggtaaaatc cttttaaaat tagagtatga aaaaacaccc
     5281 ataacagtag caaattttgt ttcattggca gaaggaacaa acatggaagt aactgatgcc
     5341 aatttaaaag gaaaaccttt tttcaacgga cttaaatttc accgtgttat tgccaatttc
     5401 atgattcaag ggggagatcc aacaggaacc ggtagcggta gcccagggta caaattcgca
     5461 gatgaaatta ccgatttaaa acacaatagc cccggtatac tatctatggc aaatgcaggc
     5521 cccggaacga atggttctca gttttttatc acccatgtag ctactccaca tttagatgga
     5581 aaacacactg tttttggaaa agttatagaa ggtcaagatg tagtgaatgc tattgcccaa
     5641 aatgatttga tggacaaagt aactatcatt cgtgtaggtg atgccgctaa aaaatttgat
     5701 gctccaaaag tttttcaagg tagattagct gctgaaatag aaaagcaaaa gaaaaaagaa
     5761 gaagcattaa aaattgaaaa agtaaaattt aacgaatacg ttaaaaaaac atttcctaaa
     5821 gcaaaaattt caccttcagg attagcttat ataattgaaa aaacaggaac cggtgaaaat
     5881 gcaaaaccgg gtaatactgt atccgtacat tatattggtg aattagaaaa tggcaaacaa
     5941 tttgacagtt cttatgaaag aaatcaacct attgaatttg tattaggaca aggtcgtgta
     6001 attccgggtt gggaagaagg aattgcgtta ttaaataaag gagcaaaagt taaactgatt
     6061 attccttatt ggttagccta cggagaaagt ggaagacctc cggttattcc tgaaaaagca
     6121 actttgattt ttaatacgga actaatcgaa attaaataat ttctatttaa aaaccgaaac
     6181 ccaaaaagtt tcggtttttt tatgttttga atttccagtc aaattcatct tgatttttaa
     6241 tgattgcacc cagttcaact tttatcactt gaccaaattc agcttgtaaa attatccaat
     6301 tctcttcatt gaagtaattg gtcacactat caaatacttc ctcttcaaat aaagtaaacc
     6361 cttttttttc caaaatagat ttcacttcaa ttaagtcttg accaatcact ttttgattaa
     6421 acaaacgtat ttcaggattt gatataatga tataacccaa tttaaaatct tcttcttgat
     6481 aaaaagttaa acgggctttg aaagcattat acaataaaat ctgattatca tcttcatctt
     6541 taaaaactga atctgccttt ccaaataaac tttcaacatc tttttgcttc atcccaaaaa
     6601 gcaattgatc taatccaaat ttaggtttaa gttccatgct tacttatttt gaataattaa
     6661 tttttttgtt aaggtattga aacgatagaa taaaaacaca gctgcagttg acaagccggc
     6721 taacaatcca atccaaaccc caatggcttt aagttctgta tacaatccca agtaaattga
     6781 aattggaaaa ccaatcaccc aataggcaac aaaagtaata tacatgggaa tttttacatc
     6841 ttgtaaacct ctcaaagctc ccaaaactac cacttgcaat ccgtctgata tttgaaacaa
     6901 agcggctaca atcaataatt gtgaagcaat ggctactact tccaaattat cttgtaaatg
     6961 aatcgtttgt tccaaattta aaaaaggaat tggaagtact ttatggaaaa taataaacag
     7021 taatgcaaaa actatttcta acaaagtagc taatagaaag atggatactg caacaattcg
     7081 caatttttta taatcctgta atcctttttg atttcccacg cgaatcatcg ctgttacact
     7141 cagtcccatc gcaaacataa acgttaaaga agccaagctc aacgctattt gattagc
//
LOCUS       DAOTCW010000113         7179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_7512_length_7179_cov_38.386440, whole genome shotgun sequence.
ACCESSION   DAOTCW010000113 DAOTCW010000000
VERSION     DAOTCW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7512_length_7179_cov_38.386440"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1339)
                     /gene="creD"
                     /locus_tag="P5335_09015"
     CDS             complement(2..1339)
                     /gene="creD"
                     /locus_tag="P5335_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HRZ75058.1"
                     /translation="MEKQTNFFQSNTAKIIMVGLLTFILLIPLQFVKNLITERADRQK
                     EVVAETSEKWGEQIYFYGPMLKIPYKTHVETKLYDPKTKEHVNSVTTELHTAYFFPEE
                     LKNTTKVTMVKPLQRSLYKSNVFTAEMAFTGNYITPNFKINNIPNEDILWNEATVVIR
                     TTNLKSIKSQVNLKLGDSMYRFEPTQHIQNDTMSTLETGLVDLRSLQQDASMDFKFSI
                     NYNGSKLIRLAPIGKTTTAHMTSNWHSPSFMGNFLPNDKHKKISAAGFEADWEVLHLN
                     RPFAQQSFDVLPNVKDYAFGVDFITTVDEYQQNERASKYGFLVIGLTFLIFFLIQSIS
                     KISIHIFQYSMIGLALLMFYTLLISITEHSSFTLAYIIAGLSVIVLISVYSLSILKDR
                     KFPVFIGVSLSGLYAFIFVIIQMEDYALLVGSVGLFLILAAVMYFSRRIDWGN"
     gene            complement(1358..1681)
                     /locus_tag="P5335_09020"
     CDS             complement(1358..1681)
                     /locus_tag="P5335_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75059.1"
                     /translation="MTKQTYASVTGKLSTQIAIASFGIGTLLFLLHQLFPKEEIIFVS
                     GFFFVLIALFVNGVVLLNLIHHFIFFRNHREYFGIKILLVMANLPIAALFFYITINSI
                     NLFTF"
     gene            complement(1750..2046)
                     /locus_tag="P5335_09025"
     CDS             complement(1750..2046)
                     /locus_tag="P5335_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRZ75060.1"
                     /translation="MKNIIQHINKAFDHRIRLGIMSVLMVNESADFTTLKELLGVTDG
                     NLASHTKALETEDYISIEKQFIGRKPNTSYRATPLGKAAFKEHINALEKLIQKS"
     gene            complement(2088..2711)
                     /locus_tag="P5335_09030"
     CDS             complement(2088..2711)
                     /locus_tag="P5335_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75061.1"
                     /translation="METKDYLKDIQDIKEMMSKSSQFISLSGLSGILAGVYALVGAWM
                     AYKTIYFDTSTMGNYKHLVISQEAVYKLLVIAAAVVILSLATGLFLSIRKAKKNNETV
                     WNSAAKRLFINFMIPMVTGGFFILFLIEKEMLGLVAPLTLLFYGLACVNASKYTLGGV
                     RYLGITMIVLGLLSTWFLGYGLLFWAIGFGFCHILYGSVMYFKYERK"
     gene            2786..3136
                     /locus_tag="P5335_09035"
     CDS             2786..3136
                     /locus_tag="P5335_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRZ75062.1"
                     /translation="MKPAEEYILNQPEPYRSILLHVQTVVEQAIPELQLKYKYNIPFF
                     YFGKKPFLYLNASHKKQFVDVAFFKGYQLTLHQDELIGEGRTLVKSLQYKSLQEIDNQ
                     VITDLLFEQWKLVK"
     gene            complement(3234..4079)
                     /locus_tag="P5335_09040"
     CDS             complement(3234..4079)
                     /locus_tag="P5335_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75063.1"
                     /translation="MKNQSLLIVFFFSVFFQQLSAQDSLVKPEKYTAHNKGKFYVFWG
                     GNRGYFTDSDIHFTGDDHDFTIYNVPSHDKPKGWHVDYISPGRMTIPQTNFRLGYFIN
                     DHYNISIGVDHMKYVMYQDRAVDINGSYPNEGTYGETLPNNQVFLTEEFLTFEHTDGL
                     NYINSEFSRVDDISSWFKLPNTDKVQINLTEGVGAGVLYPRSNVQMFGTDRYDEFHLA
                     GFGLDVKAGLNFTFFKYFFLQTELKGGYINMNDIRTTSDSRDKASQDFWFMETVIAFG
                     GIFRI"
     gene            complement(4101..4919)
                     /gene="kdsA"
                     /locus_tag="P5335_09045"
     CDS             complement(4101..4919)
                     /gene="kdsA"
                     /locus_tag="P5335_09045"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRZ75064.1"
                     /translation="MNISNIPLIKHTNSGNFFVLAGPCAIEGEEMALRIAERLVAITD
                     KLEIPYVFKGSFKKANRSRIDSFSGIGDEKALKILQKVSQTFGIPTVTDIHTNEDAAM
                     AAEYVDVLQIPAFLVRQTDLVVAAANTGKTVNLKKGQFMSPESMKHAVQKVLDCQNEN
                     VMLTDRGTMFGYQDMIVDFRGIPTMKQFATTVLDVTHSLQQPNQTSGVTGGRPDMIET
                     IAKAGIVTGVDGIFIETHFDPANAKSDGANMLHLDYFEDLMTKLVAIRKTINQF"
     gene            complement(4982..5440)
                     /locus_tag="P5335_09050"
     CDS             complement(4982..5440)
                     /locus_tag="P5335_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphooctonate aldolase"
                     /protein_id="HRZ75065.1"
                     /translation="MKVLKYSIGFILVLLLFACGATKSTIKNIDDTAPVPMLKKNNSF
                     VLTEQSQDPKYGYDQDYPINIFYRSTSNDSINQERFLKALAGPNGEKLFYKKLESCCP
                     FPTKQSEIGAGLLDVYQVNWVGCKRPLLLYFNIYEKGKLYIPMGLTAKKD"
     gene            5547..6095
                     /locus_tag="P5335_09055"
     CDS             5547..6095
                     /locus_tag="P5335_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRZ75066.1"
                     /translation="MEFITQTWHWSISGFLIGMVMLVLIYFGKTFGMSSNLRSLCAMT
                     GAGRFIPFFDWDWKEQRWNLIVVLGAMIGGFIAVHFLHNATNVTLNPKTIEQLKLLGL
                     EEPNGKLLPNALFNNSSPKIIILLIIGGFLIGFGSRYAGGCTSGHAISGLSNLQVPSL
                     KAVIGFFIGGLIMAHFLLPLIF"
     gene            6099..6512
                     /locus_tag="P5335_09060"
     CDS             6099..6512
                     /locus_tag="P5335_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRZ75067.1"
                     /translation="MKLLKYLLVGFLFGIVLTKTEAVSWYRIYEMFHFQSFHMFGIIG
                     TAILTGVIGIQFIKSKKLKDIDGQPIEILDKEDGNLRYWFGGILFGLGWALVGACPGP
                     IFILLGAGFYNVAFVLLGALIGTFLYGLIKDKLPH"
     gene            complement(6592..>7179)
                     /locus_tag="P5335_09065"
     CDS             complement(6592..>7179)
                     /locus_tag="P5335_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HRZ75068.1"
                     /translation="AQYYTNNASNIVVQGGNLVITAKAENLGGANYTSARIKTLDKFE
                     FTYGKVEVRAKLPVGGGTWPAAWMLGANFPEVGWPNCGEIDIMEHKGNEPGAIYGTLH
                     YPGNSGGNANSSSTTIANVSSEFHVYSVIWTASSIRFFKDGTLFKTFNNSNTVPFNSD
                     FFMILNVAMGGTFGGNIDPAFTQSAMEVDYVKVYQ"
BASE COUNT         2315 a         1519 c         1169 g         2176 t
ORIGIN      
        1 cctaattccc ccaatcaatc cttctcgaaa aatacatcac cgcagctaaa atcaaaaaca
       61 acccaacact acccactagc aacgcataat cttccatttg aataatcaca aaaataaacg
      121 cataaagccc cgaaagcgaa accccgataa acaccggaaa cttcctatcc ttcaaaatcg
      181 aaagcgaata caccgaaatc aacacaatca ctgaaagccc cgcaataata taagccaacg
      241 taaaactact atgctccgta atcgaaatca gcaacgtata aaacatcaac aacgctaacc
      301 caatcatcga atactgaaaa atatgaatcg aaatcttact gatagactga atcaaaaaga
      361 aaattaaaaa cgtcaaccca atcactaaaa agccatactt cgacgcccgt tcattctgct
      421 gatactcatc cacggtagta ataaaatcaa caccaaacgc ataatccttc acattcggca
      481 acacatcaaa cgattgttgt gcaaacggac gattcaaatg caacacttcc caatcggctt
      541 caaacccggc ggcactaatc ttcttatgct tgtcgttagg taaaaagtta cccataaaac
      601 taggggagtg ccagttcgaa gtcatgtgag cagtagtcgt tttaccaata ggtgccaacc
      661 gaatcaactt actaccatta taattaatcg aaaacttaaa atccatcgat gcatcttgtt
      721 gtaaagaacg caagtcaaca agtcccgtct ctagtgtcga catcgtatcg ttttgaatat
      781 gctgcgtagg ctcaaagcga tacatcgaat cgcccagttt caaattcacc tgactcttaa
      841 tacttttcaa attcgtcgta cggataacaa ccgtagcctc gttccacaaa atatcctcat
      901 tcggaatatt gttaatctta aagttgggcg taatataatt ccccgtaaac gccatctcgg
      961 ccgtaaacac attcgattta tacaaactgc gttgcaacgg cttcaccatc gtcaccttag
     1021 tcgtattctt caactcctcc ggaaagaaat aagccgtatg cagttccgtc gtaaccgaat
     1081 taacgtgttc cttcgtcttc ggatcataca gcttcgtctc cacatgagtc ttatacggaa
     1141 ttttcaacat cggaccataa aagtaaattt gttcgcccca cttttccgag gtttcggcaa
     1201 ccacctcttt ttgtcggtca gctcgctccg tgataaggtt cttcacaaat tgcagcggaa
     1261 ttaataaaat aaaggtcaat aagccaacca taatgatttt ggccgtgttc gattgaaaga
     1321 aattggtttg tttttccatg atataaataa ttaaaaatta aaaagtgaac aaattgatac
     1381 tattgatagt gatataaaaa aagagagccg caataggaag gttggccatc accaacaaaa
     1441 tctttatccc aaaatactcc cgatggtttc tgaaaaaaat aaagtgatga atcaaattga
     1501 gtaaaaccac gccattcaca aaaagtgcga taagaacgaa gaaaaaccca ctcacaaaaa
     1561 tgatttcttc cttgggaaac aactgatgaa gtaaaaacaa caaggttcct attccaaaag
     1621 aagcaatggc aatttgagta gacaatttac cggtaacgga ggcatacgtt tgtttagtca
     1681 tagtgtttgt ttagattatg ataacttaaa gtactttgct tttcaaagtt aatagataaa
     1741 aaaaaggggt taactttttt gtattagttt ttccaaggca ttaatatgtt ctttaaatgc
     1801 ggctttgcct aggggagtag cccgataact tgtattaggt ttccttccga taaactgttt
     1861 ttcaatagaa atatagtctt ctgtttctaa cgccttagta tgggaggcta aattaccatc
     1921 agtcacccct aacaattctt ttagcgtagt aaagtctgca ctttcgttca ccatcaatac
     1981 cgacatgatg cccaaccgaa tccggtgatc aaaagctttg tttatatgtt gaatgatgtt
     2041 tttcatttta cttggtttgg ctcgtcaata gctaaagaag tgggcaatta ttttcgttca
     2101 tatttaaagt acattaccga accatacaga atatggcaaa acccaaaacc gattgcccaa
     2161 aacaacaaac catacccaag aaaccaggtg gaaagtaacc ccaacacaat catggttatc
     2221 cctaaatacc gaacaccacc taacgtatac ttgctggcat tgacacacgc caagccataa
     2281 aacaataatg tcaaaggagc aaccaagcct aacatctctt tctcaatcaa aaacaagata
     2341 aaaaacccgc ccgtcaccat tggaatcata aaattgataa acaaacgttt tgccgcactg
     2401 ttccaaacgg tttcattgtt cttctttgct ttacgaatgc ttaaaaacaa tccggttgct
     2461 aaggaaagta taaccaccgc cgccgcaatg accaagagtt tgtaaaccgc ttcttgcgaa
     2521 atgaccaaat gcttgtaatt ccccatcgtc gacgtgtcga aataaatcgt tttataagcc
     2581 atccaggcac caactaaagc atacaccccg gcaagaattc ccgacaaacc acttaatgaa
     2641 ataaactgac tggatttaga catcatttct ttgatgtctt gaatgtcttt taaataatct
     2701 tttgtctcca tgtaaagtac tttgagatgc aaagtaaaat ataaattaat aaacaaccaa
     2761 acctttttta atttattttt gaaacatgaa acccgcagaa gaatacatcc tcaaccaacc
     2821 cgaaccctat cggtctatcc tgcttcatgt tcaaacggta gtagagcagg ccatccctga
     2881 actgcaattg aaatacaagt ataacattcc gttcttttat tttggtaaaa agcctttctt
     2941 gtatctcaat gccagccaca aaaaacaatt tgtcgacgtg gcgttcttca aaggctatca
     3001 attaaccctc caccaagatg aattaatagg cgagggcaga accttagtga aatccctgca
     3061 atacaaatcc ctacaagaaa tcgacaatca agtcatcacc gacctacttt tcgaacaatg
     3121 gaaactcgtt aaataaaaaa aaatgcctcc cctgaagaaa gcatttcact aagacttcta
     3181 tattctattc tctatattct attctctata ttctattctc tattctcttc cttctaaatc
     3241 ctaaatatcc caccaaaggc aatcaccgtt tccataaacc aaaaatcctg cgaagctttg
     3301 tctcttgaat cacttgtagt tctaatatca ttcatgttga tatacccgcc tttcaattcc
     3361 gtttgaagga aaaagtattt aaagaaggtg aaattcaaac cggccttcac atctaaacca
     3421 aatccggcta aatgaaactc atcataacgg tcagttccaa acatttgcac attcgaccga
     3481 ggataaagca caccggcacc aactccttcc gtcaaattaa tctgaacttt atccgtgttc
     3541 ggcaatttaa accaagagga aatatcatcc acccgtgaaa attcgctatt tatataattc
     3601 aaaccatccg tatgttcaaa ggttaaaaat tcttccgtta aaaacacctg attgttcggc
     3661 aatgtctcac cataggttcc ttcatttgga taacttccgt taatatccac tgctcggtct
     3721 tgatacatca catatttcat gtggtcaaca ccaatcgaaa tgttgtagtg gtcattaata
     3781 aagtaaccta atctaaaatt tgtttgcgga atcgtcattc tccccggact gatgtaatcc
     3841 acatgccatc ctttcggttt gtcatgtgag ggaacattat aaattgtaaa atcatgatca
     3901 tctccggtaa aatgaatatc agaatcagta aaataacctc ggtttccacc ccaaaagaca
     3961 taaaatttac ctttgttatg agcagtgtat ttttcaggtt tcaccaaaga gtcttgggct
     4021 gatagttgtt gaaaaaacac actaaaaaag aacacaatca acagcgattg atttttcaca
     4081 ggataatata aaaatttaaa ttaaaattga tttatggttt tgcgaatggc aactaattta
     4141 gtcattaagt cttcaaagta gtctaaatga agcatattcg ctccgtcgct tttagcattt
     4201 gccggatcaa aatgagtttc aataaagatt ccgtcaacac ccgtaacgat acctgctttt
     4261 gcaatggttt caatcatatc cggacgacca ccggtcactc ctgaagtttg attgggttgt
     4321 tgtaatgaat gggttacgtc taaaacagta gtagcaaatt gtttcatggt tggaatacct
     4381 ctaaaatcca caatcatatc ctgataacca aacatcgttc ctcgatcagt aagcattaca
     4441 ttttcatttt gacaatccaa cactttctga acagcatgtt tcatgctctc cggactcata
     4501 aattggcctt tcttcaagtt aaccgtttta ccggtattag cggctgccac aactaagtcc
     4561 gtttgacgaa ccaaaaaggc aggaatttgc aacacatcaa catactcggc ggccatagca
     4621 gcatcctcat tcgtatgaat atctgtcact gtcggtatcc cgaacgtttg tgatactttt
     4681 tgtaaaatct ttaaggcttt ttcatccccg attcccgaaa aactgtcaat tctcgaacga
     4741 ttggcttttt taaatgaacc tttaaacaca tacggaattt ctaatttgtc agtaatagcg
     4801 actaatcgct cggcaattcg caacgccatc tcttcgcctt caatcgcaca agggccggct
     4861 agtacaaaga aattgccact gttagtatgt ttaatcagag gaatattact aatgttcata
     4921 gtagctattt tttaaggtgc aaaaatacaa atattttaag aactaagtgt aaccaatgtt
     4981 actaatcttt tttagcagtc aaacccatcg gaatataaag ttttcccttt tcataaatat
     5041 tgaaataaag caacaacgga cgtttgcatc ccacccaatt tacttgatac acatctaaaa
     5101 ggccggcacc tatttcactt tgcttggtag gaaacggaca acagctttcg agttttttat
     5161 aaaacagctt ttcaccgttt ggtccggcta gtgctttcaa aaaacgttcc tgattaatag
     5221 aatcatttga cgtacttcgg taaaaaatat tgataggata atcttggtca tacccatatt
     5281 ttggatcttg gctttgctct gtcaacacaa aagagttgtt tttcttaagc atcggaacag
     5341 gagccgtgtc atcaatgttc ttaatggtac tttttgttgc accacaggca aatagcagta
     5401 ataccagtat aaatccgata gaatatttta aaactttcat attaattgaa ttcaatttga
     5461 atcaaaggta aaagtttttt cttataaatg aagaagatta gatttgtaat aacaaatttc
     5521 atattctatt tttgtaaaaa aaagcgatgg agttcattac acaaacttgg cattggtcca
     5581 tttccggttt cttaattgga atggtgatgt tggttttgat ttattttggc aaaacctttg
     5641 gcatgtcttc taatcttcgc tcgttatgtg ccatgacagg agcaggaagg ttcatccctt
     5701 tctttgattg ggattggaaa gaacaacgtt ggaatttaat cgtagtattg ggagccatga
     5761 ttgggggttt tatcgccgtt cattttcttc ataacgctac caatgtaacg ctaaatccca
     5821 aaacaataga acaacttaaa ctactcggcc ttgaggagcc taatggcaaa ttgttaccca
     5881 atgcgttgtt taataattct tccccaaaaa ttattatttt attaataatt ggtggttttt
     5941 taattggctt tggtagtcgc tatgccggtg gatgtacttc cggtcatgcc atttccggat
     6001 tgagtaattt acaggttcct tctttaaaag cggttatcgg tttttttatt ggcggtttaa
     6061 tcatggctca ttttcttcta cctttaatct tctaattgat gaaattactt aaataccttc
     6121 ttgttggttt tttattcgga atcgttttga ccaaaacaga agccgtttcg tggtaccgta
     6181 tttatgaaat gttccatttt caatcttttc atatgtttgg aatcattgga acagctattt
     6241 taactggagt tattggtatt caattcataa aaagcaaaaa gctaaaagac attgacggac
     6301 agcccataga aatattggat aaagaagacg ggaatctacg ctattggttt ggcggaattc
     6361 tatttggttt gggttgggca ttagttggag cctgtcccgg acctattttt atattacttg
     6421 gagccggttt ttataacgtt gcctttgtgt tgttaggagc attaatagga acgtttctct
     6481 atgggttgat aaaagataaa ttacctcatt aaaaaaagag aaaaagcgtt tgatcaattg
     6541 atacaaacgc tttttcagtt acaactaact tcttattttt tgattgacta gttattgata
     6601 tactttcaca tagtcaactt ccatcgccga ttgtgtaaag gccgggtcaa tattgccacc
     6661 aaaggttcct cccatggcaa cgtttaaaat cataaagaaa tcactattaa acggaacagt
     6721 gttgctgtta ttaaaagttt taaataaagt accatcttta aagaaacgaa tactggaagc
     6781 cgtccaaatc acagaataca catgaaactc ggaagaaaca ttcgctatag ttgtagaact
     6841 actgtttgca tttccacctg aatttccggg ataatgtaat gtgccgtata tcgcacccgg
     6901 ttcattccct ttgtgttcca tgatgtctat ttctccgcag tttggccaac caacttcagg
     6961 gaaattcgct cctaacatcc aagcagcagg ccatgttcca cctccaacag gcagttttgc
     7021 acgtacttct actttaccat atgtaaattc aaatttatcc aaagttttaa ttcgtgcgga
     7081 agtataattt gcacctccaa ggttttcagc ttttgctgta atcaccaagt ttccgccttg
     7141 aacaacaata ttactggcgt tgttggtata gtattgagc
//
LOCUS       DAOTCW010000114         7165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_7528_length_7165_cov_42.642053, whole genome shotgun sequence.
ACCESSION   DAOTCW010000114 DAOTCW010000000
VERSION     DAOTCW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7528_length_7165_cov_42.642053"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..>1083
                     /locus_tag="P5335_09070"
     CDS             56..>1083
                     /locus_tag="P5335_09070"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HRZ75069.1"
                     /translation="MKLQQLLLNSKKQQKILLSTDNFKSENANLVLAFAQRTLLEKVC
                     PFDLLKEIYPNAQIILCSSSGQVSNNCTIEKGIVVTAIEFDKTKIIATEFDILANNNI
                     DDLGEKIKEELFSKDLKSVLILSEGSFINGTELINELIKRTNKLIPIFGGLAGDEFSF
                     EKTIVGLNANASSGKIVAVGFYGEHIHFGYGSEGGWGDFGPEREVTLSDKNVLYKIGD
                     RFALDLYKEYLGKYADELPGSSLYFPLSMKENCESASVVRTILAIDEENKSMTFAGNI
                     PEGSLVRLMKGNIDKLIDAASTAAFKIKTTSKEKVQLALLVSCVGRRIVLGDRVEEEL
                     EVVKDNFG"
     assembly_gap    1084..1183
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1184..2383
                     /locus_tag="P5335_09075"
     CDS             <1184..2383
                     /locus_tag="P5335_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRZ75070.1"
                     /translation="KILNREIEIKKEFQEQLNSAKIIAEKANDAKSEFLSIMSHEIRT
                     PLNAIIGLVYIMEKENSLESFHENIEVLKHSAQNLFLLINDILDFNKIEAGKVDIEKI
                     PFDFKELIKQIAKSLQVKASENLNTIQINIDVDFQPNLISDPLRINQIITNLVSNAIK
                     FTKEGTITITVNQISIINDISTFKFEVKDTGIGIDKENFQNIFQKFSQAETKTYRQFG
                     GTGLGLVITKKLLDLLESDIQFESEIGIGTKFYFTISLPINKHFDTVVTDDLQENYKE
                     QDLHGMRVLLVEDNLINIKIAQKIMSQWNVEVDLAENGLVGVEKFNLNSYDVILMDLS
                     MPVMDGYEATSLIREKDKLIPIVALTASASFGYLDRALQIGINEYIIKPFNPKELNMK
                     LRKYYKG"
     gene            2519..>4362
                     /locus_tag="P5335_09080"
     CDS             2519..>4362
                     /locus_tag="P5335_09080"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRZ75071.1"
                     /translation="MQEQNQYTEDNIRSLDWKEHIRMRPGMYIGKLGDGSSPDDGIYI
                     LLKETLDNCIDEYVMGAGKTIEVTIKDKLVTVRDYGRGIPLGKVVDVVSKMNTGGKYD
                     SKAFKKSVGLNGVGTKAVNALSNYFRVESVRDNQQKAAEFSAGNLTLEEDIQETTKRR
                     GTKVAFIADETIFKNYKYRNEYIIKMLKNYCYLNTGLTIYYNGEKYYSENGLKDLLEE
                     NITEEDMIYPIIHLSGDDIEIAITHSKSQYSEEYHSFVNGQNTTQGGTHLNAFRESIV
                     KTIREFYKKDFDPSDIRKSIVSAIAVKVEEPVFESQTKTKLGSTDIGPKGPTVRTFIN
                     DFISKKLDNFLHKNPEVADLLLRKILQAERERKELSGIRKLAKDRAKKASLHNKKLRD
                     CRVHLTDAKNPRTLESTLFITEGDSASGSITKSRDVNTQAVFSLRGKPLNSYGMSKKI
                     VYENEEFNLLQAALNIEESMEDLRYNNIVIATDADVDGMHIRLLLITFFLQFFPELIK
                     DGHLYILQTPLFRVRNKKETIYCYSEQERVDAIEKLKPKPEITRFKGLGEISPDEFKN
                     FIGESIRLDPVMLDKATSIETLLEFYMGKNTPDRQEFIINNLKVELDVV"
     assembly_gap    4363..4462
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4481..7159
                     /locus_tag="P5335_09085"
     CDS             4481..7159
                     /locus_tag="P5335_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HRZ75072.1"
                     /translation="MIPEDENEELNESSQEQGFDDVKVFSNKHFYENDENPEDTITKV
                     TGMYKDWFLDYASYVILERAVPAIEDGFKPVQRRIMHSMKELDDGRYNKVANIVGHTM
                     QYHPHGDASIGDAMVQIGQKDLLIDMQGNWGNILTGDSAAASRYIEARISKLGHDILY
                     SPKITEWGMSYDGRRAEPINLPVKFPLLLAQGAEGIAVGLSTKVLPHNFIELIEASIK
                     ILKNKPFTIFPDFPTAGIADVSNYNDGLRGGRVRVRAKISQLDKQTLVITQIPFSTNT
                     GTLIDSILKANEKGKIKVKKIEDNTAAEVEILIHLPPGVSPDKTIDALYAFTACETSV
                     APLGCVIENHKPLFIGVSDMLKISTHRTVDLLKRELEIQLDELENKWHFSTLEKIFIR
                     EEMYIDFKLYSDREALYVYMYDRFKPFLKSFVREINDDDLQKLTQIPMIRITRFDSDK
                     ADDAIAKLEAEMELVKHHLNNIIDFAIDYFQKLKDKYGKGRERQTELRSFDTIEATKV
                     VLRNTKLYVNKEEGFLGTGLKKDEYVADCSDIDDVIVFLRDGKMMVTKVDDKKFIGKD
                     IIHIAIFDKNDKRTIYNMIYRDGKNGSTFIKRFNVSGVTRDKFYDLTQEKPGSQTLYF
                     SANPNGEAEVVTILLRQVGSVKKLKWDLDFADIAIKGRASRGNTVTKYPIKKVELKEK
                     GISTLRPRKIWFDDTVQRLNVDGRGDLLGEFRPNDRILVINQAGKLKTIIPELTTHFD
                     EEMIVLEKWHPNKPISAIYYDGEKDRYYVKRFLVENENKEEIFISEHEKSQLEIVSTD
                     WRPVAEVIFAKVKGIQKENQTVDFEQFIAIKGIKALGNQLTTDKIKQVNLLEPLPYEE
                     PEEEVPEEMEVSGEDNVTDDIQTELDDDGQITLSLE"
BASE COUNT         2519 a         1016 c         1308 g         2122 t
ORIGIN      
        1 ttttttgttt ttaataaata cttttcattc ctttatacac cttaatccta ctatagtgaa
       61 attacaacaa ctgttactca atagtaaaaa acagcaaaaa atacttttat caacagataa
      121 tttcaagtct gaaaatgcta atttagtttt agcttttgca caaagaacat tattagaaaa
      181 agtttgtcct tttgatttgt taaaagaaat ttatcccaat gctcaaatca tactttgttc
      241 ctcttccggg caagtttcaa ataattgtac tatagaaaag ggaattgtag ttacagcaat
      301 tgaatttgat aaaacaaaaa ttatagcaac tgaatttgat attttagcta acaataatat
      361 agatgaccta ggtgaaaaga ttaaagagga attattttca aaggatttaa aatctgttct
      421 tattttatca gaaggaagtt ttatcaatgg tactgaactt attaatgagt taattaagcg
      481 aactaataaa ttaataccaa tttttggtgg tttagcaggt gatgaattca gttttgaaaa
      541 aactatagtt ggtttaaatg caaacgcttc atctggaaaa attgtagcgg taggttttta
      601 tggtgagcat attcattttg gatatggttc tgaaggtggt tggggcgatt ttggaccgga
      661 acgagaggta actttgtctg ataaaaatgt tctctataag ataggtgatc gttttgcttt
      721 agatttatat aaagaatact taggcaaata tgcagacgaa ttacctggtt catcgttata
      781 ttttccactt tcaatgaaag agaattgtga atcagcttca gtagttagaa caatattagc
      841 tatagatgaa gaaaataaat caatgacatt tgcaggaaac attcccgaag gttcacttgt
      901 aagattaatg aaaggaaata tagataaact cattgatgca gcatcaactg ctgcctttaa
      961 aattaaaact acatcgaaag aaaaagttca gttagcactt ttagtgagtt gtgttggaag
     1021 aagaatagtt ttaggagata gagtggaaga agaattggaa gttgttaaag ataattttgg
     1081 tgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaaattc ttaaccgaga
     1201 aatagaaata aaaaaagaat ttcaagagca attaaattca gctaaaataa tagctgaaaa
     1261 agcaaatgat gctaaatcgg agtttctatc tatcatgagt catgaaataa gaacaccact
     1321 taatgctatt attggtttgg tttatatcat ggaaaaagaa aattctttgg aaagttttca
     1381 tgaaaacatt gaagttttaa agcattctgc acaaaattta tttctcctaa taaatgatat
     1441 tcttgatttc aataaaattg aagccggaaa ggtggatata gaaaaaatcc cttttgattt
     1501 taaagaacta atcaaacaaa ttgcaaaatc tttgcaagtt aaagcgtctg aaaatttaaa
     1561 tacgattcaa ataaatattg atgttgattt tcaacctaat ttaattagtg accctttacg
     1621 aattaatcaa attattacca atttagtttc taatgcgatt aaatttacta aagagggtac
     1681 aattaccatt acggttaatc aaatctcaat aattaatgac atttcaacat ttaaatttga
     1741 agtaaaagat actggtattg gaattgataa agaaaatttt caaaatattt ttcaaaaatt
     1801 ttcacaagca gaaacaaaaa cttatagaca atttggagga acaggtttag gattagtaat
     1861 aaccaagaag ttacttgatt tattagaatc agatattcaa tttgaaagtg agattggtat
     1921 tgggacaaaa ttctatttta caataagtct acccataaat aaacactttg atacagttgt
     1981 tacagatgat ttgcaagaaa attataaaga acaagatcta catggaatgc gggtgttatt
     2041 agtagaagat aatttgatta acattaaaat tgctcaaaaa ataatgagtc aatggaatgt
     2101 ggaagttgat ttagctgaaa atggtcttgt tggtgttgaa aaatttaatt tgaattctta
     2161 tgatgttatt ttaatggatt tatcaatgcc ggtaatggat ggttacgaag caacttcatt
     2221 aattagagaa aaagataaat taattcctat tgttgcccta acagcctcag cctcatttgg
     2281 atatttagac agagcacttc aaattggaat taatgaatat attataaaac cttttaatcc
     2341 gaaagaactc aatatgaaat tgagaaaata ttataaaggt tagttttttt atatttggtt
     2401 gctttaacaa tataaaatgt atgttttttg tcaatattat tattgataac ttcttttttt
     2461 tattctttta tttcctattg atttcactta cattagcaga aactcgatat tacccaaaat
     2521 gcaagaacaa aatcaatata ccgaagacaa tattcgttcc ctcgattgga aagaacacat
     2581 ccgaatgcga cccggaatgt atatcgggaa gctcggtgat ggttcttcac ctgatgatgg
     2641 tatttacata ttgttgaaag aaacattaga taactgtatc gatgaatacg tgatgggagc
     2701 tgggaaaact attgaagtta ccatcaaaga taaattagta accgtccgtg attatggtcg
     2761 tggaattccg ctaggaaaag ttgttgatgt tgtttcaaaa atgaataccg gtggtaaata
     2821 cgattccaaa gccttcaaaa aatcggttgg tttgaatgga gtagggacaa aagcagtaaa
     2881 tgcgttgtcc aattatttcc gtgtggaatc ggttcgtgac aaccaacaaa aagctgctga
     2941 attctctgcc ggaaatttaa cgttggaaga agacattcaa gaaacaacca aacgccgagg
     3001 tactaaagta gcctttattg ccgatgaaac tatttttaaa aactacaaat accgtaatga
     3061 gtacatcatc aaaatgctca aaaattattg ttacctcaat acgggattaa caatatatta
     3121 caacggtgaa aagtattatt ccgaaaatgg tttaaaagat ttattggaag aaaatattac
     3181 cgaggaagat atgatttatc caatcattca tctttcaggc gacgatattg aaatagccat
     3241 tacccatagt aaatcgcaat attctgaaga ataccattca tttgtaaatg gtcagaatac
     3301 gacgcaaggc ggaacgcatt taaatgcttt tcgtgaatca atcgttaaaa ccattcgtga
     3361 gttttataaa aaagattttg acccttcaga tatcagaaaa tcaattgttt ctgctattgc
     3421 agttaaagta gaagaaccgg tttttgaaag tcaaacgaaa actaaattag gttccaccga
     3481 tatcggtccg aaagggccaa cggtgcgtac attcatcaat gatttcatat ccaaaaaact
     3541 cgataatttt ttacataaaa atccggaagt agccgattta cttttacgta aaattctaca
     3601 agccgaacgt gaacgtaaag aactttcagg tattcgaaaa ttagcaaaag accgagccaa
     3661 aaaagcaagt ttgcacaata aaaaattacg cgattgtcga gtgcatttaa ccgatgctaa
     3721 gaatccacga acgttagaaa gtacactttt tatcaccgaa ggagattccg cttccggttc
     3781 gattactaaa agtcgcgatg tgaatacgca agcggttttt agtctacgtg gaaaaccgtt
     3841 gaattcgtat ggcatgagta agaaaatcgt ttacgaaaac gaagaattca acttattgca
     3901 agccgcttta aacattgaag aatcgatgga agatttgcgt tataataaca tcgtaattgc
     3961 taccgatgcc gacgtggatg gtatgcacat tcgcttgttg ttgattactt tttttcttca
     4021 attttttccg gagttaatca aagacgggca tttatatatt ttgcaaacgc cgttgttccg
     4081 tgttcgaaat aaaaaagaaa ccatttattg ttactccgaa caagaacgtg tagatgccat
     4141 cgaaaaatta aagcccaaac ctgaaatcac tcgattcaaa ggattaggag aaatttcgcc
     4201 cgacgaattc aagaatttta ttggcgaaag cattcgatta gatccggtga tgttggataa
     4261 agccacttcc attgaaacgt tattggagtt ttatatgggt aaaaatacgc cggatagaca
     4321 agagtttatt atcaataatt tgaaagtgga attggatgtt gtnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnaacgaaga cgaagaaaac atcatccccg aagatgaaaa
     4501 tgaagaactc aacgagtcaa gccaagaaca aggttttgac gatgtaaaag tttttagtaa
     4561 taaacatttt tacgaaaacg acgaaaaccc cgaagacacc atcaccaaag tgactggaat
     4621 gtacaaggat tggtttctcg attatgcttc ttacgtaatt ttggagcgtg ctgttcctgc
     4681 cattgaagat ggttttaaac cggttcaacg ccgaatcatg cactcgatga aagaactaga
     4741 cgacggtcgt tataacaaag tagcgaatat cgttggtcat acgatgcaat atcatccaca
     4801 cggagatgcc agtattggtg atgcgatggt acaaatcggt cagaaagatt tgctgattga
     4861 tatgcaagga aactggggaa atatactaac tggtgacagt gccgccgctt cccgttacat
     4921 cgaagcacga atttcgaaat tgggtcacga tattttatat tctcctaaaa ttaccgaatg
     4981 gggaatgtcg tatgatggtc gtcgtgccga acctattaat cttccggtaa aatttccact
     5041 tttgttagca caaggtgctg aaggaattgc cgttggttta tctactaaag tgttgccaca
     5101 taattttatt gaattgattg aagcttcgat aaagatttta aagaacaaac catttacaat
     5161 ttttcctgat tttccaaccg caggaattgc tgatgtttcc aattataacg atggattgcg
     5221 aggcggaaga gttcgtgttc gtgcgaagat ttctcaatta gataaacaaa cgttggtgat
     5281 tacacaaatt ccgttttcta ccaataccgg aacgttgatt gacagtattc tgaaagccaa
     5341 tgaaaaagga aaaattaaag tcaaaaaaat agaagacaac acggctgcag aagttgaaat
     5401 tttaattcat ctaccaccgg gcgtttcgcc ggataaaacc attgatgcat tgtatgcatt
     5461 cactgcatgc gaaacttccg ttgctccgtt aggttgcgtg attgaaaatc ataaaccgtt
     5521 gtttatcgga gtttcggaca tgttgaaaat ttcgacacat cgaacggttg atttgttgaa
     5581 acgggaattg gaaattcaat tggatgaact tgaaaacaaa tggcattttt ctaccttaga
     5641 aaaaatcttc attcgagaag aaatgtacat cgacttcaaa ttgtattctg atagagaggc
     5701 tttatatgtt tacatgtacg atcgattcaa accatttttg aaatcatttg ttcgagaaat
     5761 taatgatgat gatttgcaga aattaactca aattccaatg attcgtatca cacgtttcga
     5821 ttcagacaaa gcagatgatg ctattgcgaa gttggaagct gaaatggaac tagtaaaaca
     5881 tcatttaaat aacatcatcg attttgctat tgattatttt caaaagttaa aagacaaata
     5941 cggaaaagga agagaacgtc aaaccgaatt gcgaagtttt gatacgattg aagctacaaa
     6001 agtggtattg agaaacacga agttatacgt caacaaagaa gaaggtttcc ttggaaccgg
     6061 actcaaaaaa gacgaatatg tagccgattg ttcggatatt gatgatgtaa ttgtcttttt
     6121 acgagatgga aaaatgatgg taaccaaagt ggatgataaa aaattcatag ggaaagacat
     6181 cattcacatt gccatttttg ataaaaacga taagcgaacg atttataata tgatttatcg
     6241 cgatgggaaa aatggttcta cttttatcaa acgatttaat gtttcaggag ttaccagaga
     6301 taaattttac gatttaacac aggaaaaacc gggttcacaa acgttgtatt tttctgccaa
     6361 tccgaatgga gaagcagaag tggtgactat tttattgcgt caggttggaa gtgtaaaaaa
     6421 attgaagtgg gatttagatt ttgccgacat tgctattaaa ggaagggctt ctcgcggaaa
     6481 tacggtgacc aaatacccaa ttaaaaaagt cgaattaaaa gaaaaaggaa tttcaacact
     6541 tcgtccacgt aaaatttggt ttgatgatac ggttcagcga ttaaacgtgg atggacgagg
     6601 cgatttactt ggtgaattca gacccaatga ccgtattttg gttatcaatc aagccggaaa
     6661 attaaaaacc attattccgg aattgactac ccattttgat gaagaaatga tagtgttgga
     6721 aaaatggcat cctaataaac ctatttcggc gatttattat gacggagaaa aagaccgata
     6781 ttatgtcaaa cgtttcttgg ttgaaaatga aaataaagaa gaaatcttca tttcagaaca
     6841 tgagaaatca caattagaaa ttgtttctac tgattggcga ccagttgctg aagtaatttt
     6901 tgcaaaagta aaaggtattc aaaaagaaaa tcagacggtt gattttgagc agtttatagc
     6961 aattaaagga attaaagcac tcggaaatca attgacaaca gataaaataa aacaagttaa
     7021 cttgttggaa ccacttcctt atgaagaacc ggaagaagaa gttccggagg aaatggaagt
     7081 ttcaggtgaa gataatgtta cagacgatat tcaaactgaa ttagatgacg atggacaaat
     7141 tacattgagt ttagaataaa aaaaa
//
LOCUS       DAOTCW010000115         7065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_7691_length_7065_cov_50.729815, whole genome shotgun sequence.
ACCESSION   DAOTCW010000115 DAOTCW010000000
VERSION     DAOTCW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7691_length_7065_cov_50.729815"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="P5335_09090"
     CDS             complement(<1..1091)
                     /locus_tag="P5335_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRZ75073.1"
                     /translation="MSQKRLFLLDAYALIFRGYYAFIKNPRINSKGMDTSAIMGFMNA
                     LMEVIKREKPDHLAVAFDKGGSDYRYEMYKEYKAHRDETPEAIKIAVPYIQEILKAMQ
                     IPIMEKAGFEADDLIGTLAKQAEKEGFQVFMVTPDKDFAQLVSENIFMYKPARMGNDI
                     EIWGIPEVLAKFEIERPEQVIDYLGMMGDSADNIPGFPGVGEVTAKKLLKEFGTMENL
                     LENTDKLKGALKDKIENNKELGILSKKLARILLDCPVTFDATDFELSKPDVEKTDAIF
                     QELEFRQMKAQFDKWFGTGKEYDEINTNGNGDSNSNEIGNGTEKVVKIASTKKSNEDQ
                     FDLFGFSDDDSDEAKPNSYYATLETTEHF"
     gene            1190..2458
                     /locus_tag="P5335_09095"
     CDS             1190..2458
                     /locus_tag="P5335_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRZ75074.1"
                     /translation="MILCYFAQKYTVLFRLLFFVLFVGIVEVYAFQAFKTISRIKWVQ
                     YTYILLSVVILIYIIYSFTQFDRRVGQTQQTLFTLGLLLITVIPKIILTLFMFGEDFF
                     RVISGLISHFFVSGENSSFLPQRRKFVSQVALGIAAVPFLSLLYGVTKGKYNFKVIKK
                     ILFFPDLPESFDGTTITHISDIHSGSFDDAEKIQYAVDLINQQKSDVILFTGDIVNTH
                     ATEMQQWIEMFKGIHNPKFGKFSVLGNHDYGEYIDWPTQKAKDENFEAIKGLHQQIDF
                     KLLLNEHVKLKNGSDEIAIVGVENWGHNFKKVGDLNKASDGLSVNDFKILMSHDPSHW
                     EYEVKNNQKHFHLTLSGHTHGMQFGIEIPGIIKWSPVQYVYKQWAGLYENFGRYIYVN
                     RGFGFHAYPGRVGIWPEITVIELKKGEKVV"
     gene            2532..2828
                     /locus_tag="P5335_09100"
     CDS             2532..2828
                     /locus_tag="P5335_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRZ75075.1"
                     /translation="MSKFGELINAQVPVLIDFYTEWNESSVAMHPVIRDVAAALGDKA
                     KVIKIDVDKNQELADALRIKGLPTLMIYKEGQMVWRQSGELDANTIIGLVQEQV"
     gene            complement(2825..3463)
                     /locus_tag="P5335_09105"
     CDS             complement(2825..3463)
                     /locus_tag="P5335_09105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRZ75076.1"
                     /translation="MNFLWIKTNRLIKYIFSNYVWNIPNVEKKVYLTFDDGPTPEITE
                     WVLEQLKTYNAKATFFCIGNNIAKHPEIFKRLLNEGHTIGNHTQNHVKGWETENNTYF
                     GEVEKCDEVISKQTNIDSKLFRPPYGKIKPSQSNYLRKLGYKIIMWDVLSADFDTTIS
                     KEKCLENVLKNVESGSIIVFHDSIKAFPNLEYTFPKTLEFLSKNGYICDRIN"
     gene            complement(3504..6704)
                     /locus_tag="P5335_09110"
     CDS             complement(3504..6704)
                     /locus_tag="P5335_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRZ75077.1"
                     /translation="MMTFDFKKWNTIIGWLTFTIALITYTLTVEPTLSFWDCGEYIAT
                     SAKLQVGHPPGAPLFQMMGAFFALFAPEASKVALMVNMMSVFSSAFTILFLFWSSSIL
                     LKKIIAQFKEITSIEEIAILIASFVGALAFTFTDSFWYNAVEAEVYAMATLLIALLFW
                     LGLRWEQEMHTPRGNKWLLLICLVIGLSFGVHFMALLTIPSIGLLYFFKNYKTITFKS
                     FIIANLVVVAILLFIFKLLLPLTMALFGKTEVFMVNTFGLPFDSGTIITALALIAFFY
                     FGLKYTRSKNLPQFNTILLCILFVLIGFSTWMMLPIRANANVVINENRPSDAREVLAY
                     YNREQYPQNPLFYGPQFSDMFAGLDPDEPYLDKAPNYERDYKLGKYVITNNYENATQN
                     SHNAHKAILPRMWSGDHAQNYMDFTNVLEFKINPEYAHEQELVDIVSQFRKAYSSGRL
                     DTEDYVSFLKSYGNYLIVEKPTTSDNIKFMTQFQFGYMYVRYLLWNFVGRQSDVQGKY
                     DNEGNWLSGIDFIDEIFLGSQKNLPQDVLNNKGRNVYFFLPFILGIIGILFHAKRDLK
                     SFYVLLALFLFTGIALKIYLNERPFEPRERDYALVGSFYVFAIWIGLGVYAIFDFIKK
                     WLKPKFALPLVFVSTLFAAPVLLASQNWDDHDRSNKYTALANAKGYLESCEPNAILFT
                     IGDNDTFPLWYAQEIEGIRRDVRVVNTQLFMTDWHIDQMKAKAYDSEPLPISFDRSHY
                     VGDKLDYCVYNEITQNRWELKNFLDFIKSDDPNTMLEMQSGQSVHFFPTNKIRIPIDK
                     NVIIKNKVVAEKYYDSIVPYIDVDIKDRAIYKARIMMFDVIANNNWKRPIYFTGGSFT
                     EEDYLWMKDYLQLDGLVYKLVPIKTPVDEENSPFDMGQIDSDKMYDIVMKWDWGNGER
                     TDINHDTETRRNSITYRTNLSRLMQQLIVEGKTDKAKKVIELAINKMPIDLYGYYTMV
                     EPFTGGYYDIGEVEKARALLQKLASKYQDNLNYYSGIVPSEQNDRIVDIVTNLERYRS
                     LLYVMKEKEDIEFYEKNKKIFNGYNKRFERFNRKDE"
     gene            6943..7013
                     /locus_tag="P5335_09115"
     tRNA            6943..7013
                     /locus_tag="P5335_09115"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6975..6977,aa:Gln,seq:ttg)
BASE COUNT         2328 a         1169 c         1147 g         2421 t
ORIGIN      
        1 taaaaatgtt cggtagtttc taaagtagca taatatgaat taggtttcgc ttcatcggaa
       61 tcatcatcac taaatccaaa caaatcaaat tgatcttcgt tggatttttt ggttgaagct
      121 atcttgacta ctttttcggt gccatttcca atttcattgc tgttgctgtc accatttcca
      181 tttgtattga tttcatcata ttcttttcct gttccaaacc atttatcaaa ttgggctttc
      241 atttgtcgga attccaactc ctgaaaaata gcatcagttt tttctacatc gggtttagat
      301 aattcaaaat cggtagcatc aaacgttacc ggacaatcta gtagaattcg ggccagtttt
      361 ttagataaaa tccctagttc tttattgttt tcgattttat cttttaaggc accttttagt
      421 ttgtcggtgt tttctaaaag attttccatc gtaccaaatt ctttcaataa tttctttgcg
      481 gtcacttctc caacaccagg aaaaccggga atattatcgg cagaatcgcc catcattccc
      541 aaataatcta tcacttgttc cggacgttca atttcaaatt tggccaacac ttccggaatt
      601 ccccaaattt caatgtcgtt ccccattcgt gcgggtttgt acataaaaat attttcagaa
      661 accaactgag caaaatcttt atccggtgta accataaaaa cttgaaaacc ttctttttcg
      721 gcttgttttg ctagagttcc aattaaatca tcagcttcaa aacccgcttt ttccataatc
      781 ggaatttgca tggcttttaa tatttcttga atatagggta cggcaatttt aatggcttcc
      841 ggcgtttcgt cacgatgagc tttgtattct ttgtacattt catagcgata atcgcttccg
      901 cctttatcaa aagcaactgc caaatgatct ggtttttccc ttttgataac ttccattaaa
      961 gcgttcataa aacccattat tgcggaagtg tccattcctt tggaattgat acgcgggttt
     1021 ttgataaagg cataataacc acgaaaaatc aaggcataag catcaagaag aaaaaggcgt
     1081 ttttgtgaca taaaacaaaa atttaaaaac gtaaaaataa gcaatcttag cggaatctta
     1141 agatgtaatt aaggatataa attttaatta attattaact attaagccat tgattctttg
     1201 ttactttgct caaaaatata ctgtgttatt tagattactc ttttttgttc tgtttgttgg
     1261 aattgttgaa gtctatgctt ttcaggcatt taagacgatt tctcgtatca aatgggttca
     1321 atatacttat attctgttaa gtgtagtgat tttgatttat atcatttatt ctttcacgca
     1381 gtttgataga agagtcggtc aaacacaaca aactcttttt acactgggat tgcttttaat
     1441 aacagtaatt cctaaaatta ttttaaccct atttatgttt ggggaagatt tttttcgcgt
     1501 gatttcaggg ttgatttcgc atttctttgt gagtggagag aatagttcgt ttctaccaca
     1561 aagaagaaaa ttcgtcagtc aagttgcatt aggaattgca gcagttccat ttttatcgct
     1621 tttatatgga gtaacaaaag ggaagtataa ttttaaagtt atcaaaaaaa ttctattctt
     1681 tcctgattta ccggaaagtt ttgatggaac aaccattacg catatttccg atattcattc
     1741 cggaagtttt gatgatgcag aaaagattca atatgctgtc gatttaatta accaacaaaa
     1801 atcagatgtg attttattta ctggagacat tgtcaacacg catgctacag aaatgcaaca
     1861 gtggattgaa atgtttaaag gaattcataa tccaaaattc ggaaaatttt ctgtattagg
     1921 aaatcacgat tatggtgaat acatcgattg gccaactcaa aaagctaaag atgaaaattt
     1981 tgaagcaata aagggtctac accaacaaat tgatttcaag ttattattga atgaacatgt
     2041 aaagcttaaa aacggttcag atgaaattgc aattgtagga gtggaaaact ggggacataa
     2101 tttcaaaaaa gtaggcgatt taaacaaagc ttcggatgga ttgtcagtga atgattttaa
     2161 aattttaatg agccacgatc caagccattg ggaatatgaa gtgaaaaata accaaaagca
     2221 ttttcattta accctatcgg gacacaccca tggaatgcaa tttggaattg aaataccggg
     2281 tattattaaa tggagtcctg ttcaatatgt ttacaaacaa tgggctggat tatatgaaaa
     2341 ttttggacga tatatctatg taaatcgtgg gtttggattt catgcatatc cgggaagagt
     2401 aggaatttgg cctgaaatta cggttattga actaaaaaaa ggagaaaaag tagtgtaatt
     2461 cgttaaaaat gctacatttg tatattcttt attaaagaat agatttaaat tttatttaaa
     2521 atttgtcaaa tatgtcaaag tttggtgaac ttattaacgc tcaagttccg gtgctaattg
     2581 atttttacac cgagtggaac gaatcttcag ttgcaatgca tccagttatt agagatgtag
     2641 ccgcagcatt gggtgataaa gcaaaagtga ttaaaataga cgtggataaa aatcaggaat
     2701 tggctgatgc tttgcgaatc aaaggacttc ccaccttaat gatttataag gaaggtcaga
     2761 tggtttggag acaatccggc gaattagatg ccaacactat aattggtctg gttcaagaac
     2821 aagtctaatt aattctatcg cagatatatc catttttgga taaaaattcc aacgttttgg
     2881 gaaaagtata ttccaaattc ggaaatgctt ttatactgtc gtgaaaaacg attatactac
     2941 ctgattcaac atttttaagt acgttttcta aacatttttc tttagagatt gtggtgtcaa
     3001 aatcagcact taaaacatcc cacatgatta ttttatagcc taattttcgc aaatagtttg
     3061 attgtgaggg ttttattttt ccatagggtg gtcgaaaaag ttttgaatct atattagttt
     3121 gtttgctaat tacttcatca catttttcta cttctccaaa ataggtgtta ttttctgttt
     3181 cccaaccctt tacatgattt tgggtgtgat ttccaatggt atgaccttca tttaataacc
     3241 ttttgaaaat ttccggatgt ttcgcgatat tatttccaat gcaaaaaaaa gttgccttag
     3301 cgttgtaggt tttcagttgt tctagcaccc attctgtaat ttccggtgtt ggaccatcat
     3361 caaaagtcag gtaaactttc ttttcaacgt tgggaatatt ccaaacataa tttgaaaaaa
     3421 tatatttaat aagccgattc gtttttatcc aaagaaaatt catggctgtt tataaaaaag
     3481 agccactttc atggctctcg gttttattca tcttttctat taaaacgctc aaaacgttta
     3541 ttatatccgt tgaatatttt tttgtttttc tcgtagaatt caatatcttc tttttctttc
     3601 atgacatata aaagcgatcg gtatcgttct agatttgtga caatatcgac gattctgtca
     3661 ttttgctctg aagggacaat tccactataa taattgagat tatcttggta ttttgaagca
     3721 agtttttgca ataatgctct ggctttttct acttcaccaa tatcataata tcctcctgta
     3781 aaaggttcaa ccattgtata atagccatat aaatcaattg gcattttatt aatggcaagt
     3841 tcaattacct ttttagcttt atctgttttg ccttcaacaa tcagctgttg cattaatcga
     3901 gataaatttg ttctgtaagt gatactgttt cttcgcgttt ctgtgtcatg attgatgtct
     3961 gttcgttcac cattacccca atcccatttc attacaatgt catacatttt atctgaatca
     4021 atttgaccca tatcaaaagg actgttttct tcatcaacag gagttttaat aggaactaat
     4081 ttgtacacta atccatctaa ttgaagatag tctttcatcc ataaataatc ttcctctgta
     4141 aaactacctc ctgtaaaata gattggtctt ttccaattgt tgtttgcaat aacatcaaac
     4201 atcattatac gagctttata aattgcacga tcttttatgt caacatcaat ataaggaaca
     4261 atggaatcat agtatttttc agcgacaact ttatttttta taatgacatt tttgtcaatt
     4321 ggaattctaa ttttattggt cggaaaaaag tgtacgcttt gtccactttg catttctaac
     4381 attgtatttg gatcatcact ttttataaag tctaaaaagt ttttcaattc ccaacggttt
     4441 tgagtgattt cattatacac acaataatct aatttatcac ctacataatg gcttctgtca
     4501 aaagaaatag gtaaaggttc ggaatcatat gcttttgctt tcatttgatc aatatgccaa
     4561 tccgtcataa ataactgggt gtttactacc cgtacatctc gccggattcc ttcaatctcc
     4621 tgggcatacc acaatggaaa agtgtcgtta tcaccaatag tgaataatat ggcatttggt
     4681 tcacaagatt ctaagtaacc ttttgcattt gctaatgcag tatatttatt agaacggtca
     4741 tggtcatccc aattttgaga agccaataaa acgggtgctg caaataaggt agatacaaaa
     4801 actaatggca aagcaaattt tggtttcaac cattttttta tgaaatcaaa aattgcgtaa
     4861 acacctaatc ctatccatat agcaaaaaca taaaaagacc ctactaaagc ataatctctt
     4921 tcccgtggtt caaaaggtct ttcattcaag tatattttta aagcgattcc agtaaataag
     4981 aacaaagcta aaagaacata aaagctttta agatctcttt tagcatgaaa aagaatgcca
     5041 ataatgccta gaataaaagg caaaaagaaa tagacatttc gtcctttatt atttaaaaca
     5101 tcttgaggca gatttttttg agaacccaaa aatatttcat caataaaatc aataccgctt
     5161 agccaatttc cttcattgtc atattttcct tgaacgtcac tttgacggcc tacaaagttc
     5221 cacaagaggt aacgaacata catatagcca aattgaaatt gtgtcatgaa cttgatgttg
     5281 tctgaggtag ttggtttttc aacaatcaaa taatttccgt aagatttcaa aaatgaaaca
     5341 taatcttcag tatctaatct gccagaacta taggcttttc tgaattgtga aacaatatcc
     5401 accaattctt gttcatgagc atattctgga tttattttga attctaacac attggtaaaa
     5461 tccatgtaat tttgagcgtg gtctccactc cacattcttg gcaaaatggc tttgtgtgca
     5521 ttatgactgt tttgagttgc gttttcataa ttatttgtaa tgacatattt gcctaattta
     5581 taatcacgtt catagtttgg agctttgtct aaataaggct catctggatc taatccggca
     5641 aacatatctg aaaactgagg tccgtaaaac aaaggatttt gcgggtattg ttctctatta
     5701 taataggcta aaacttctcg ggcatctgaa ggcctatttt cgttgataac aacattggca
     5761 ttagcccgaa ttggcaacat catccaggtt gaaaatccta ttaatacaaa aagaatacac
     5821 aataagattg tgttaaattg cggaaggttt ttactacggg tatattttaa accaaaatag
     5881 aaaaaagcaa tgagagctaa agcggtaata attgtccctg aatcaaaagg taaaccaaac
     5941 gtgttgacca taaacacttc ggttttacca aatagagcca tggttaatgg aagtaataat
     6001 ttgaaaataa acaaaaggat tgctactact actaaattag caataataaa acttttgaat
     6061 gttattgttt tgtagttttt gaaaaagtaa agtaaaccaa tagaaggaat agtcaacaga
     6121 gccataaagt gaacaccaaa ggacagtcct ataactaaac agattaacaa taaccatttg
     6181 ttgcctcttg gtgtgtgcat ttcttgttcc catcgtaaac ccaaccaaaa taataatgca
     6241 attaataagg tggccatagc ataaacttct gcttcaacgg cattgtacca aaaactatcg
     6301 gtaaatgtaa aggctaaagc tcctacaaaa gaggcaatta aaattgcaat ttcttcaata
     6361 gaagtaattt ccttaaactg tgctattatt tttttgagca aaatggagga tgaccaaaat
     6421 aggaacaaaa ttgtaaacgc acttgaaaac acagacatca tgttcaccat taaagctact
     6481 tttgaggctt ctggagcaaa taaagcaaaa aaggcaccca tcatttgaaa taaaggagct
     6541 cccggaggat ggcctacttg aagtttcgca gacgtagcga tgtactcacc acaatcccaa
     6601 aaactaagag ttggttcaac agttaaagta taagttatta atgcaatggt aaaggttaac
     6661 catcctatta tggtattcca ttttttgaaa tcaaatgtca tcatttgtgt atctttgttt
     6721 gaaagaaata atagttacaa agaaactatt tttttatgta aacaacgttg gcttaacaaa
     6781 attttctcac tacgatttat aatgaaaaca aaataaatta aaaatgaatt tattgaggtt
     6841 gaggaagtat tacaaatagt gtaaatattt ttcataaaaa ggcattaaat atttgcttta
     6901 aataaaaaat agtataaatt tgcacccgca ttcaagcgat tttggtccat ggtgtaatgg
     6961 taacacaact gtttttggtg cagtctttca aggttcgagt ccttgtggac caacagaaaa
     7021 aaccactctt tatgagtggt ttttttgttt tttaaaaata aaaaa
//
LOCUS       DAOTCW010000116         7065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_7692_length_7065_cov_45.608417, whole genome shotgun sequence.
ACCESSION   DAOTCW010000116 DAOTCW010000000
VERSION     DAOTCW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7692_length_7065_cov_45.608417"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="P5335_09120"
     CDS             complement(<1..1445)
                     /locus_tag="P5335_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRZ75078.1"
                     /translation="MIKKLCKISVFTLLLLISCSKEDEMKEELITIYNQQEPLTARQI
                     NDNINSYNQSGEKFYWKKASTHMLWSAVLKGNNLVTVGFGVNSSDFDRTKSTSSNQIQ
                     NELINLIKRYEGDQNEHLVVNQDAYLNLIDVTITKQETLEALRQNKFVRYVEPADYRY
                     FSFENQGNPKDNQIQSDSGCGYGTSTISSADFTTISPNAQVPWAFYKHGIPSAWGYSS
                     GSGITIGVVDSGLSSQQSLMNASFNSGLSSGRSVQRFGTYVDSIWPWSTTTDGVNDLC
                     GHGTSMASVATAPRNGNGLPVGVAYNANLVTYRASSDVVLNGYHEQNGVKNAFTALGN
                     RTDVKIISMSMGHIFSVGKIEDGVRYANNRGKLILCAGGTSTSFTNFVGVIFPAWMPE
                     TVAVTGIKEASTYQKCDVCHSGSEIDFTIMMQRSGSNNTVPVLSYYNNQADYVGGSSV
                     ATATTAGIAALVWAKNPSWTKAQVLTKMKQSS"
     gene            complement(1592..2290)
                     /locus_tag="P5335_09125"
     CDS             complement(1592..2290)
                     /locus_tag="P5335_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HRZ75079.1"
                     /translation="MRKITFILVTVSLMITNFVQAQEKSNEKVTADCERNWQVRLRGI
                     GIFPDQSATIGIIGGDIAISNAYVPELDFTYFFTEHFAAELILGTAKHDVNTVGSDIS
                     AIGGPTSFDVDLGSVMLLPPTLTAQYHFFPMKEKKFKPYVGAGINYTIFYNVKEGDVV
                     KGIEYDNAVGFAGQVGFDLLFTDKYFVNFDVKKLFLKTDVTVDASNLAAGLSIPAEVT
                     IDPWIIGIGVGMKF"
     gene            2431..2883
                     /gene="smpB"
                     /locus_tag="P5335_09130"
     CDS             2431..2883
                     /gene="smpB"
                     /locus_tag="P5335_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRZ75080.1"
                     /translation="MQRMINILNKRAKFDYEFLEKFDAGIVLTGTEIKSIRLGKASIT
                     ESFCEFHNGELFVINSQVEEYLYGTHYNHKAKSERKLLLNKKELKSLEKSMQNKGLTI
                     IPLRLYVNEKGYAKMEIALCRGKKTYDKRETIKDRDTKREIDRMKKSF"
     gene            2964..3602
                     /locus_tag="P5335_09135"
     CDS             2964..3602
                     /locus_tag="P5335_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75081.1"
                     /translation="MKKILGFIAFSFLLSCSSSNTTIVNSWRDPNTSLANEQFKKVLV
                     VALLKDDVTRRVVETKFQEYNPGFKPSYNFLHQGNKDMTQEMLIKLLNLEKFDGVVTL
                     RLVDTRVETDYVPGMNTSLYYGNPYYNSYGTMFGGWYGYYSPYYYDPGYYVENTYYFI
                     ETNIFSLKNNNLIWSGTTKSSNVSGQIDETAGNIINEIVTQMRKDGSLPPIK"
     gene            3705..4751
                     /locus_tag="P5335_09140"
     CDS             3705..4751
                     /locus_tag="P5335_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRZ75082.1"
                     /translation="MKNLLKNAREQKNLKTREAAKLLEIDQALVSKFENGSRKPTREQ
                     IPKLASILEIDYETLMVAWLKEKILYEIGSDELALKAIHVTEEHIKNNLSFNRKEISN
                     SLQLVLNEIDGLKKFLNQKSSNDRLRIAQTLELEYTFESNRIEGNMLTLHETDLIISE
                     GLTVSGKSMREHLEAINHKEAIGYIKQLMERGFPFNERELLSIHNLILRGIIPEDAGS
                     YRRVQVMITGSQHMPPKQNLVTQEMEDYFKWYESQKSNLHPVILAAKMYERLVTIHPF
                     NDGNGRTSRLVMNLILLQHGYVIANIKGNYDNRMKYYNALVSAQTKNNKEDFLLYIAQ
                     IEKESLERYLSMIN"
     gene            complement(4784..5425)
                     /locus_tag="P5335_09145"
     CDS             complement(4784..5425)
                     /locus_tag="P5335_09145"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRZ75083.1"
                     /translation="MKDTSKHQGLRNQLVKLLEGKGIVDKNVLEAIQKIPRHLFLDSG
                     FEQYAYQDKAFPISAGQTISQPYTVAFQTQLLEVKKGDKVLEIGTGSGYQTAVLCTMG
                     AKVYSIERQLELFKKTSLLLPKLGIRPKHLSFGDGYKGLPDYAPFDSIIVTAGAPTIP
                     KPLMEQLKIGGRLVIPLGKNEQIMTLLIRKNETQFEKHEHGEFRFVPLLEDKN"
     gene            5582..6550
                     /locus_tag="P5335_09150"
     CDS             5582..6550
                     /locus_tag="P5335_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRZ75084.1"
                     /translation="MLKIGVLGAGHLGKIHLRLLKQSSKYELVGFYDENQENGAKIAA
                     EFGYKQFDTIAKLIHAVDVIDIVTPTLSHYKCAKVSIKSGKHVFIEKPIATTVEEAEE
                     IITLAKEYKVIGQVGHVERFNPAFIAVKNQIHNPMFIETHRLAEFNPRGTDVPVVLDL
                     MIHDIDAILSVVKSKVKAVHASGVSVLSQSPDIANARIEFENGCVANVTSSRISMKNM
                     RKSRFFQKDAYISVDYLDKVCEVVKMKDAPEVPGDFDMILQNAEGEKKQIYFDNPEVS
                     LNNAILDELETFADAINNNTTPVVTLEDGTEALRVAYMIIDSMNEN"
     gene            6623..>7065
                     /locus_tag="P5335_09155"
     CDS             6623..>7065
                     /locus_tag="P5335_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRZ75085.1"
                     /translation="MKTIAVIGAGTMGNGIAHTFAQSGFKVKLIDKFEKSLEKGMETI
                     AANLDRMVAKGSISEEDKHQTISNIITYTDINDGVVGCDLVVEAATENVGLKMNIFKQ
                     LSDICDHNVILATNTSSISITQIAAQVVHPERVIGMHFMNPVPIM"
BASE COUNT         2522 a         1144 c         1131 g         2268 t
ORIGIN      
        1 gaggattgtt tcatcttcgt caaaacctgt gctttagtcc aagatggatt ttttgcccat
       61 actaatgcag caattccggc agtagttgcc gttgccacag atgatccgcc tacataatca
      121 gcttgattgt tataataact caacaccgga actgtattgt tgcttcctga acgttgcatc
      181 ataatagtaa agtctatttc acttccacta tgacaaacat cgcatttttg atacgttgaa
      241 gcttctttaa ttccggttac cgcaacagtt tccggcatcc aagccggaaa aataacaccc
      301 acgaaattag taaaagaagt tgaagttcca ccggcacaca aaattaattt tcctctattg
      361 ttggcatatc gaaccccatc ttcaattttt ccaacagaaa aaatatgccc cattgacatt
      421 gaaattattt tgacgtctgt tctgtttcct aatgcagtaa atgcattttt aactccattt
      481 tgttcatggt agccgttcaa aacaacatcg gatgaggcac gataagttac taaatttgca
      541 ttataagcaa ctccaacggg taaaccattt ccgtttcgag gagctgtagc aacagatgcc
      601 atgctagttc cgtgaccaca taaatcattt acaccatcgg ttgttgttga ccaaggccaa
      661 atagaatcta cataagtacc aaatcgttgt acagaacgcc cggaagaaag tcctgaatta
      721 aaactagcat tcatcaagga ctgttgtgaa gataaacctg aatcgacaac accaatagtt
      781 attccacttc ccgagctata accccaagct gatggaattc catgtttata aaatgcccaa
      841 ggaacctgag cgtttggtga aatagtcgta aaatctgccg aactgattgt tgatgtacca
      901 tatccacaac ccgaatcaga ttgaatttga ttatcctttg gatttccttg attttcaaaa
      961 gaaaaatagc gataatctgc cggttcaaca taacgtacaa atttattttg tcgaagtgct
     1021 tcaagtgttt cttgctttgt aatggtaaca tctattaaat tcaaataggc atcttgattt
     1081 acaaccagat gttcattttg atcaccttca tatcttttga ttaaattgat taattcattt
     1141 tgaatttgat ttgaagatgt agattttgtt ctgtcaaaat ctgagctatt tactccaaaa
     1201 ccaacggtaa ccaaattatt acctttcaaa acagcactcc ataacatgtg tgttgaagct
     1261 tttttccaat agaatttttc accgctttga ttgtaagaat tgatattgtc gttaatctgt
     1321 cgagcagtta aaggctcttg ttggttataa attgtaatca actcttcttt catttcatct
     1381 tcttttgaac atgaaatcaa taataaaaga gtaaaaactg aaattttaca taattttttt
     1441 atcatacttt taatttttta gcttaagatt cacgaatata ataattattc gctcttaaat
     1501 ttaaaaagtt aataaaaaaa taaaggtcaa aaccaaattg gcaatgaccc ctacttctcg
     1561 acacgacgtc gaatactaac caataattta tttagaattt catacctaca ccaattccaa
     1621 taatccaagg atcaatagta acctccgctg gaatacttaa tcctgcggct aaatttgaag
     1681 catcaactgt tacatcagtt tttaagaata attttttaac atcaaagttt acaaaatatt
     1741 tatctgtaaa taataaatca aaacctactt gacctgcaaa accaactgca ttgtcatatt
     1801 caataccttt aacgacatct ccttctttaa cattatagaa aatagtataa ttgatacctg
     1861 caccaacata tggtttaaat tttttctctt tcattgggaa aaagtgatac tgagcagtta
     1921 atgttggagg taataacatt acacttccta aatcaacatc aaaacttgtt ggcccaccaa
     1981 tagcagagat atcagaacct actgtgttta catcatgttt tgcggttcct aaaattaatt
     2041 cggcagcaaa atgttccgta aaaaaataag taaaatctaa ttcaggtaca taagcattag
     2101 aaatggctat atcaccacca atgattccaa tggttgcaga ttgatccgga aaaataccaa
     2161 ttcctcttaa tcgcacttgc caatttcttt cacaatcagc cgttactttt tcatttgatt
     2221 tttcttgtgc ctgaacaaaa tttgtaatca ttaaagatac agttactaga ataaaagtaa
     2281 tttttctcat tattaaattg ttttaaaatt attgaccaaa aataatttta acttataatt
     2341 taaaaactga taaaaatcat atctcaaaaa tattttctag agtaataatt tttaaataat
     2401 cctcattttt gttcatacat ttgcaacatt atgcaaagaa tgataaacat actgaataaa
     2461 agagccaaat ttgattacga atttttggag aaatttgatg ccggaattgt tttaaccggt
     2521 acagaaatta aatctataag attgggaaaa gcatcaatca cagaaagttt ttgtgaattt
     2581 cacaatggtg aattatttgt aattaattca caagttgagg aatatttata tggcacacat
     2641 tacaatcaca aagctaaaag tgaacgtaaa cttttattaa acaaaaaaga attaaaaagt
     2701 ttagaaaaaa gtatgcaaaa taaaggtctt acaatcattc cattgcgact atacgtcaac
     2761 gaaaaaggat atgccaaaat ggaaattgct ttgtgcagag gtaaaaagac ctatgataaa
     2821 agagaaacca taaaagacag agataccaag cgtgaaattg atagaatgaa aaaaagtttt
     2881 taacgtattt ttttaaaagt ctatctgatt attctaattt aatttctatt tttaaacaat
     2941 aataaacttt tcaaaataat tttatgaaaa aaattctggg atttatagca ttttcttttt
     3001 tactttcttg cagtagtagt aacacaacaa tcgttaatag ctggagagat ccaaacacat
     3061 cgttagccaa tgaacaattt aaaaaagtat tagtggtagc actgttgaaa gatgatgtta
     3121 ccagaagggt tgtagaaact aaatttcaag aatataatcc aggatttaaa ccttcctaca
     3181 attttttgca tcaaggaaac aaggatatga cacaagaaat gttaattaaa cttttaaatt
     3241 tagaaaaatt tgatggtgtt gtaacattgc gattagtaga cacgcgtgtg gaaacagatt
     3301 atgttccggg tatgaacact tcattgtatt atggcaatcc ttattacaac agctatggca
     3361 ctatgtttgg cggttggtat ggctattatt caccttatta ttatgatccc ggttattatg
     3421 ttgaaaacac ctattatttt atagaaacca atattttttc attaaaaaac aataatctaa
     3481 tttggtcagg aacaacaaaa tcatcgaatg tatccggtca aattgacgaa actgccggaa
     3541 atatcattaa tgaaattgtt actcaaatga gaaaagacgg ttcattaccg ccaataaaat
     3601 agcaccttta aaaaaatatt ttttcgcaag taataaacaa aaattacttg cgtttttttt
     3661 gtgcgtccag catatgtgag tatttttatc atctgattaa tatgatgaaa aatttattaa
     3721 aaaatgcccg tgaacaaaag aatctaaaaa ctcgtgaagc agcaaaactt ttggagatag
     3781 atcaagcatt ggttagtaaa tttgaaaatg gttcgagaaa accaacacga gaacaaattc
     3841 ctaaattagc ttccatttta gagattgatt atgaaacctt aatggttgct tggttaaaag
     3901 aaaaaatttt atatgaaatt ggttcggatg agttagcctt aaaagcgata catgtaactg
     3961 aagaacatat caaaaataat ctctctttta atcgaaagga aatatcaaat tcattgcaac
     4021 tagttttaaa tgaaattgat ggtttaaaga aatttttaaa tcaaaaaagt agtaatgatc
     4081 gtcttagaat tgcacaaact cttgagttag aatatacatt tgaaagtaac cgtattgaag
     4141 gaaacatgtt gactttacat gaaaccgatt taattatcag cgaagggtta accgtttccg
     4201 gaaaaagtat gcgtgaacat ttagaagcaa ttaatcacaa agaagccatc ggatatatca
     4261 agcaattgat ggaaagaggc tttccattta atgaacgaga attgctctct atacacaatt
     4321 taattcttcg tggtattatt ccggaagatg ccggaagtta tcgcagggtg caagtaatga
     4381 ttaccggaag tcaacacatg ccaccaaaac aaaatttggt tacacaagaa atggaagatt
     4441 attttaaatg gtatgaaagt caaaaaagca atttacatcc ggtgatttta gctgcaaaaa
     4501 tgtacgaaag attagtcaca attcatcctt ttaatgatgg aaatggaaga acttcccgtt
     4561 tagtcatgaa cttaatttta ctacaacatg gttatgttat agccaatatt aaaggtaatt
     4621 atgataatcg aatgaagtat tacaatgcat tagtatctgc tcaaacaaaa aacaataaag
     4681 aagacttctt actctatatt gcacagattg aaaaagaaag tttagaacga tatttaagca
     4741 tgataaatta acaagaaact agcaaaattt gagtttaatt aattcaattc ttatcttcta
     4801 acaaaggaac aaatcgaaac tcaccatgtt catgtttttc aaattgtgtt tcattttttc
     4861 gaattaacaa agtcattatt tgctcgtttt ttcccaaagg aataactaat cgtccaccaa
     4921 tttttaactg ttccatcaat ggtttgggaa ttgttggagc tccggcagta acaataatac
     4981 tatcaaaagg agcataatca ggtaatcctt tgtatccatc accaaatgat aaatgtttgg
     5041 gtctgattcc taattttggt aataataatg atgttttttt aaacagttcc aattgtcttt
     5101 ctattgaata tactttagct cccattgtgc acaaaactgc cgtttgataa ccagaacccg
     5161 ttccaatttc taatacttta tctccctttt ttacttctaa taattgagtt tgaaatgcaa
     5221 cagtataggg ttgcgaaatg gtttgtccag cacttatcgg aaaagctttg tcttggtacg
     5281 catattgttc aaaaccggaa tctaaaaata aatgtcgagg gattttttgg atagcctcca
     5341 gcacgttttt gtctactatg ccttttcctt ccaatagctt gaccaactga tttcggagtc
     5401 cttggtgttt agaagtatct ttcaatttaa gtaatcttta ttttggtaaa aatagtggta
     5461 aaaaatccaa tttacaaaat ctaaagttga agaataaata ttgataaact tcttatcaat
     5521 ttgaagtttt taaacattgt attttctcat attattacta tttttgataa aatttacttt
     5581 tatgctaaaa attggcgttt taggtgccgg acaccttgga aaaatacact tacgattact
     5641 caaacaatcc tcaaaatacg aattagttgg cttttatgat gaaaatcaag aaaacggggc
     5701 aaaaatagca gccgaatttg gatataaaca atttgacaca attgctaaat taattcatgc
     5761 ggtggatgta attgatattg taactcctac cctttcgcac tacaaatgtg caaaagtttc
     5821 aatcaaatcg ggtaagcatg tttttattga aaaacccatt gcaacaaccg ttgaagaagc
     5881 agaagaaatc atcactttag ctaaagaata taaggttata ggtcaagtgg gacatgtaga
     5941 acgattcaat cctgcgttta tcgctgtgaa aaatcaaatt cacaacccga tgtttattga
     6001 aactcatcgt ttggccgaat ttaatcctcg aggaacggat gttcctgtag ttttagattt
     6061 aatgattcat gatattgatg cgattttgag tgttgtaaaa tctaaagtaa aagccgttca
     6121 tgctagtggt gtttccgttt taagtcagtc acccgacatc gccaatgcga gaattgaatt
     6181 tgaaaatggt tgtgtagcca atgtaacttc gagccggatt tcgatgaaaa acatgcgaaa
     6241 atcacgtttt tttcaaaagg atgcctacat ttctgtagat tatttagaca aagtgtgtga
     6301 agtggtaaaa atgaaagatg ctccagaagt tccgggtgat tttgatatga ttttacaaaa
     6361 tgccgaaggc gagaaaaaac aaatttattt tgataatccg gaagtttctt taaacaatgc
     6421 tattttggat gaattagaaa cttttgccga tgcaattaat aacaatacta ccccagtggt
     6481 aacgttggaa gacggaacgg aagcattacg agttgcatat atgattattg attcgatgaa
     6541 cgagaattag aaataaccgc aaattcgcaa attatgattt aataaaattt gcggtattaa
     6601 attttaaaaa aaataaaata gaatgaaaac aattgcagta atcggagcag gaacaatggg
     6661 taacggaatt gctcacactt ttgctcaaag cggatttaaa gtaaaactaa ttgataaatt
     6721 tgaaaaatca ttagaaaaag gaatggaaac tattgccgca aaccttgacc gaatggtagc
     6781 caaaggaagt atttcggaag aggataaaca ccaaaccatt tcaaacatca ttacctatac
     6841 cgatatcaat gacggcgttg tgggttgcga tttggtagtt gaagcagcaa ccgaaaacgt
     6901 tggtttaaaa atgaatatct ttaaacaatt gagtgacatt tgcgaccata atgtgatatt
     6961 agcaaccaat acttcttcca tttctattac acaaattgcg gcacaagtag ttcatccgga
     7021 acgagtaatt gggatgcatt ttatgaatcc ggtgccaatt atgaa
//
LOCUS       DAOTCW010000117         6979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_7824_length_6979_cov_39.377672, whole genome shotgun sequence.
ACCESSION   DAOTCW010000117 DAOTCW010000000
VERSION     DAOTCW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7824_length_6979_cov_39.377672"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..844
                     /locus_tag="P5335_09160"
     CDS             71..844
                     /locus_tag="P5335_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75086.1"
                     /translation="MKSLKYILDFYIHSSLHVALAVYSLVRITEYNLQFLHNENIAYT
                     VFYGTIVGYNFLKYFQSFLNGNFYAKQHRFLIGITLLSLVITVFYFLQLPTSAQLFLL
                     WTFCLVVIYPFLRKQGWLKLFWVSICMAEITVHFPAILYQVNNLDLFLLGLQRFFIVF
                     ALLIPFEILDSETDDKILKTIPQRIGIWNTKMFGFVAIVFFLGIEFSKPTFDYISLWI
                     GLITALAIWFSSTNRNHYYTSFWVECIPILWYIFYLILI"
     gene            959..>2061
                     /locus_tag="P5335_09165"
     CDS             959..>2061
                     /locus_tag="P5335_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75087.1"
                     /translation="MKIITKILLFFLIFFGANTTLLAQDIFSAKAYLLTDEFGGVTRY
                     YNCNTNGACDPGWTSGWDNTTVSTVTVGSTFQFGGNVITYDDWTNWSDATLYYRIYLD
                     GSAPGSFIPINLTQNINNICSVSDNAKRETVGTPEVITAPTTEGIYIFEIYFQARYTL
                     DLPPDDIKYYSNLSANYKAKLNVIQPIAAFESYAFVSLNGTPTFYDLDGASGSANPGL
                     PDFLGTFQPGLAFRLGAEQKIYKKASNHTVCECSSWYYFYEDNPLLDPIESDFPLPSN
                     GADFTANYNGIFKKSAVLPNLAGSFDATGSTANTDYNNLINSTTTIEKYQNTSLGLEA
                     EIIFPNCIGFYRIAIALLTKVSTNGNCSDASSF"
     assembly_gap    2062..2161
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2162..3996
                     /locus_tag="P5335_09170"
     CDS             <2162..3996
                     /locus_tag="P5335_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS sorting signal type C domain-containing
                     protein"
                     /protein_id="HRZ75088.1"
                     /translation="LLTKVSTNGNCSDASSFIYFRDINQSPLFKYDNTTDVNLNPKKP
                     VNSLSTSDFYITRIRIYDTNIPEWNGSWSIPPDKGSNVIFHSDYITAINGDLTVNTII
                     VDDGVTVTVSNGGHIECYNNAITNGTGKIVVKNQGNFVQRCDKSTIIRPNIEMEKISR
                     PMSDWDYVYWGAPVSENIYSQIPGFLDKRYRWQSGTATGSWQNLGTTLPGQGFITRIA
                     NVAPFNVTPTAINFTMKGTANNGYVYVNVDSYDSSSIIYGNAILLSNPYPCAIDAATF
                     LDHPNNTELGGTLSFWTSFTPISTSNPGPDTYNYNEADYATWNRTGGTGTKASTDTTG
                     NDSLRPTGKIASGQGFFVHAFADGQIEFDNSMRLVSNVNTQFFRTSQNALANLEPEKH
                     RFWLNFKDNSNAFRQMLVGYIENAFDGIDRNFDGTSITISPIDLYSLVDDKKLNIQGK
                     ALPFNENDIIPLGYKVVSEGNYTISIDSFDGLFTEQPIFLKDKFLNTINALKESSYTF
                     SSSAGTFHNRFEIVYQNSNILGITQDVLVGNSVVIETKNGISIQASDTIQSIAIFDLL
                     GRKIYQKEKVEQTHLLLNDFSKGNQVYIVKVKTNTFEETKKIIY"
     gene            complement(4108..4665)
                     /locus_tag="P5335_09175"
     CDS             complement(4108..4665)
                     /locus_tag="P5335_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75089.1"
                     /translation="MKNLFFFVIFISAIFFTSCSEDELDVELQTTLSQDLDVIATSGS
                     DRMDSHKSAITDSEYPFSKSAILSLDNEDTHQYLNKIKNVKIKRLTYRVEDYGTSHGT
                     LSAILYADEREIHRVEDLLARDDAGEAIVFEIVDVDGKISQMESALLNNKNLTLTITG
                     NRSCSCSMTFKVRVTADVEAIANPL"
     gene            complement(5133..>6979)
                     /locus_tag="P5335_09180"
     CDS             complement(5133..>6979)
                     /locus_tag="P5335_09180"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRZ75090.1"
                     /translation="SGEALDRLSSIFFNLNSAETCDEMQKIAQEVSPLLTAFSNDISL
                     NEDLFKRVKSVYNQKDKLTLTTEQSTLLDKKYKSFTRNGALLSQDKKVRFREIDTELA
                     KLKLTYGENVLAETNNYQLQITNEADLKGLPDGAKEMAASLANSKGLDGWVFTLDFPS
                     YLPFVTYVENRELRKEIAIAAGKKAFQNNEFDNKEITLKIAKLRYERANLLGYKTHSH
                     FVLEERMAQNPEKVKLFLNDLLEKAKPAAHREFAELTAFAAALDGIDQLQKWDGAYYS
                     EKLKQKLFSLDDELLKPYFKLENVLNGAFTVAEKLFGIKFKEVFDIDKYHEDVQTFEV
                     LDFQDELVAVFYADFFPRKGKRNGAWMTSFKPQYIKKGVNERPHVSIVCNFTPPTETK
                     PSLLTFNEVTTLFHEFGHALHGMLANTTYPSLSGTSVYWDFVELPSQVMENWCYEPEA
                     LALFAKHYETGEVIPQQYVEKIKESASFLEGMATLRQLSFGLLDMAYHSNNPTEITNI
                     KAFEKEVFEGTSLYPDVEENCMSVSFSHIFQGGYSSGYYSYKWAEVLDADAFAYFQEK
                     GIFNKEVATKFKDNVLSKGGTELPMELYKKFRGQEPKVEALLKRAGLI"
BASE COUNT         2244 a         1093 c         1089 g         2453 t
ORIGIN      
        1 tccttttttt attaatacaa tttattttca gtataatttt gttacttgca gtattagtaa
       61 aacaaaatag ttgaaaagct taaaatatat actcgatttt tatattcatt cttctttaca
      121 tgtagcgtta gctgtttatt cattagtacg aataacagaa tataatttgc aattcctaca
      181 taatgaaaat atagcgtata ctgtttttta tggaacaatt gtgggatata attttttgaa
      241 atatttccaa tcatttttaa atggaaattt ttatgcaaaa cagcatcgtt ttttgattgg
      301 aattacactt ttatctttag tcattaccgt tttttatttt ttacaacttc caacatcggc
      361 acaactcttt ttgctttgga cattttgtct agtggttatt tatccatttc taagaaaaca
      421 gggttggctt aaattgttct gggtgagtat ttgtatggca gaaattacag tacattttcc
      481 ggcaatttta tatcaggtga acaacttaga tttatttttg cttggattac aaagattttt
      541 tatagttttt gctttattga ttccgtttga aattttagat agtgaaacgg atgataaaat
      601 tttaaaaacg attccgcaac gaattggaat ttggaataca aaaatgtttg ggtttgtggc
      661 aatagtcttt tttttaggaa ttgaattttc aaaacctacc tttgattata tcagtttatg
      721 gattggattg attacagcat tagcaatttg gttttcttct actaaccgaa accactatta
      781 tacttctttt tgggtagaat gtatcccaat tttatggtat atattttatc taattctcat
      841 ttaatcagtt ttttattaac acgaaaacgt tttcggaacc tctaaaaatt aaaaaaatga
      901 aatttttagg ttaattgtac taatttagta gatgttaaaa tgcaagtaat tgctaaacat
      961 gaaaattata actaaaattt tactcttttt tcttattttc ttcggtgcta atacgacgtt
     1021 attagctcaa gatatatttt cagcaaaagc ctatttgctc actgatgaat ttggtggtgt
     1081 aactcgttat tacaattgca ataccaatgg agcttgtgat ccgggttgga catcaggttg
     1141 ggacaacaca actgtttcta ccgttactgt tggttcaact ttccagtttg gaggaaatgt
     1201 aattacttat gatgattgga cgaattggtc tgatgctaca ttgtattata gaatttatct
     1261 tgatggttca gctccgggaa gttttatacc tatcaatctg actcaaaata taaataatat
     1321 ttgtagtgtt tcggataatg ccaaacggga aacagtagga actcctgaag taattactgc
     1381 tccaacaaca gaaggaatat acatttttga aatttatttt caagcaagat atactctgga
     1441 tttaccacca gatgatatta aatattactc taatttaagt gcaaattaca aagcaaaact
     1501 aaatgtgatt caacccatag ctgcatttga atcgtatgct tttgtgagtt taaacggaac
     1561 cccaacattt tatgatttag atggtgcttc tggaagtgct aatcccggat tacctgattt
     1621 tttaggcacc tttcaacccg gcttggcttt tagattaggt gcagaacaaa aaatatacaa
     1681 aaaagcatca aatcacaccg tttgcgaatg ttcttcatgg tattattttt atgaagataa
     1741 tccactttta gatccgatag aatcagattt tcctttgcca agtaatggtg cagattttac
     1801 agcaaattac aatggtattt ttaaaaaatc agccgtgctc cccaatttgg cggggagttt
     1861 tgatgctacg ggttccacgg caaatacaga ttataataat ttaatcaaca gcacaactac
     1921 tattgagaaa tatcaaaata cttcgttggg tttagaagcc gaaataattt ttcctaattg
     1981 tatcggtttt tacagaattg ccattgcttt attaactaaa gtcagtacca atggaaactg
     2041 tagcgatgcg agtagtttta tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nctttattaa ctaaagtcag taccaatgga aactgtagcg atgcgagtag ttttatctat
     2221 tttagggata ttaatcaatc accccttttt aaatacgata acacaacgga tgttaattta
     2281 aatcccaaaa aaccggtaaa ctcactttca actagtgatt tttatattac cagaattcga
     2341 atttatgata ctaacatacc agaatggaat ggaagttggt caattccgcc cgataaagga
     2401 agtaatgtta tttttcatag tgattatata acagcaataa atggagattt aacagtaaat
     2461 accataatag tagatgatgg ggtaacggtt actgtttcaa atggtggtca tatagaatgt
     2521 tataataatg caatcacaaa cggcaccggt aaaattgttg ttaaaaatca aggaaatttt
     2581 gtgcaacggt gtgataaatc aaccattatt cgtccgaata tagaaatgga aaaaatttca
     2641 agacctatgt cagattggga ttatgtttat tggggagctc cggtaagcga aaacatttac
     2701 agtcagattc ccggtttttt agacaaacga taccgttggc aatccggtac agcgacagga
     2761 agttggcaaa atcttgggac aacattacca ggacagggtt ttattactag aattgctaac
     2821 gtagctcctt ttaatgtaac tccaacagcc attaatttta ctatgaaagg gacagcaaat
     2881 aatggatatg tttatgtaaa tgttgatagt tacgatagtt cttcaattat ctatggaaat
     2941 gcgattttac tttcaaatcc ctatccatgt gcgattgatg ctgcaacttt tttagaccat
     3001 cctaataata cggaattagg tggaacgttg agtttttgga cttcttttac accaatttcc
     3061 acctctaatc ccggaccgga tacctataat tataatgagg cggattatgc cacatggaat
     3121 agaacaggag gaacaggaac aaaagcttcc acagatacaa cgggaaatga ttcattacga
     3181 ccaaccggta aaattgcttc aggtcaagga ttttttgttc atgcttttgc agacggtcaa
     3241 atagaatttg ataattccat gcgtttagtc tctaatgtta atacgcaatt ttttagaact
     3301 tctcaaaatg ctttagcaaa tttagagcct gagaaacatc ggttttggtt aaattttaaa
     3361 gataattcaa acgcatttag gcaaatgttg gttgggtata ttgaaaatgc tttcgatggt
     3421 attgacagaa attttgacgg aactagtata acgataagtc caatagattt atattctttg
     3481 gttgacgata aaaaattaaa tattcaagga aaagcacttc catttaatga aaatgatata
     3541 attcctttgg gctataaagt tgtttccgag ggcaattata ccatttcaat agacagtttt
     3601 gatggtttgt ttacagagca acctattttt ttgaaagaca aatttttgaa tacaattaat
     3661 gctttaaaag aaagttcgta tactttttct tcttcagctg ggacatttca taatcggttt
     3721 gaaattgttt atcaaaactc aaacatattg gggataactc aggatgtgct agttggaaat
     3781 tcagttgtta tagaaactaa aaatggaata tccatccaag cttccgatac gattcaatcc
     3841 atagctattt tcgatttatt gggaagaaaa atttatcaaa aagaaaaagt agaacaaact
     3901 catctattac taaacgattt ttcaaaaggg aaccaggttt acattgttaa agtgaaaaca
     3961 aatacttttg aagaaacaaa aaaaattatt tattaatatt tcaaaacact agtttgattt
     4021 atttataatg aaacacctat ctcctttaaa agagataggt gttttagttt aatttatatc
     4081 attaagaatt ttcaagtttt agaatcctta caaaggatta gcaattgctt caacgtcagc
     4141 agtaactctc actttaaaag tcattgaaca actacaagat ctattaccgg tgatggtgag
     4201 cgttaaattt ttattgttta aaagagcact ttccatttgg cttatttttc catctacatc
     4261 aactatttca aatactattg cttctcctgc atcatctcga gctaataaat cttcaactct
     4321 gtgaatttct ctttcgtcgg catataaaat agcggaaaga gtcccgtgtg aagttccata
     4381 gtcttcaact ctataagtca atctttttat tttaacattt ttaattttgt ttaaatattg
     4441 atgagtatct tcattatcta aactcaaaat agctgattta ctaaaaggat attcagaatc
     4501 agtaatagca cttttatgac tgtccattct atcagaacca gaagttgcaa tcacatctaa
     4561 atcttgagac agggtggttt gcaactcaac atccaattca tcttcactac aagatgtgaa
     4621 gaaaatagct gatataaaaa tcacgaaaaa aaatagattt ttcataatat ttcatttaaa
     4681 agttaataaa tttctttgct tttttttaag tattcttgct ttatttttat aagaatacag
     4741 gttttaatta aggttagcta gtctataaca aattttcttt gttattaaaa taggttatca
     4801 tttgttattt cagaattggg gttttgttta aaataacata ttacaaagtc aaacacactc
     4861 ataaacttat tctataaatg ttttcaaaga aactagaaga ttaacttttt gcacatctaa
     4921 tttaagatta taataggtgt ttttgtttga taaaaatcat tgtttaatta ctaaaaattt
     4981 agtaaattgt agaattgcaa attttataaa ttagattaaa atcaattttg atttttaaaa
     5041 aaaaggatta agtatagaag tgaatttaat ttttaaggtt tgataataaa aaaaccgaag
     5101 ttttcacttc ggtttccatt attttgtttt aattatatta aaccggccct ttttaataga
     5161 gcctccactt tcggttcctg acctctaaac tttttatata attccatcgg taattctgtt
     5221 ccacctttcg aaagtacatt atccttgaat tttgtggcaa cttctttatt aaaaatgcct
     5281 ttttcttgaa aataagcaaa tgcatcggca tctagcactt ccgcccattt gtaactgtaa
     5341 tacccggaag aataacctcc ttgaaaaata tgcgaaaatg aaacactcat gcagttttct
     5401 tctacatccg gataaagtga agttccttca aacacttcct tttcaaaagc tttgatattg
     5461 gtaatttcag ttggattatt actgtgatat gccatgtcta gtaggccaaa actcaattgt
     5521 cgtaaagtgg ccattccttc caaaaagctg gcactttctt tgattttttc cacatattgt
     5581 tgcggaatca cttcacctgt ttcgtaatgt ttggcaaata aagccaaggc ttcaggttcg
     5641 tagcaccagt tttccatcac ttgacttggt aattccacaa aatcccaata tactgaagtt
     5701 cccgataaac tcggataggt cgtgttggcc aacatcccat gcaaagcgtg tccgaattca
     5761 tgaaataaag tcgtcacttc gttaaaagtt aggagggaag gtttagtttc cgtcggaggt
     5821 gtaaaattgc aaacaattga aacgtgaggt cgttcgttaa cgcccttttt gatatattgt
     5881 ggtttaaaag atgtcatcca agcaccattt cgtttccctt ttctaggaaa aaaatcagcg
     5941 taaaaaacag caactaattc atcttgaaaa tcgagtactt caaaagtttg cacatcttcg
     6001 tggtatttgt caatatcaaa aacttcttta aacttaattc cgaataattt ctcggcaacg
     6061 gtaaaagctc cgttcaacac attttctaat ttgaaatagg gttttaataa ttcatcatct
     6121 aaactgaata atttttgttt taatttttca gaataataag ctccatccca tttttgaagt
     6181 tggtcaattc catctaatgc tgcagcaaaa gcagtcaatt ccgcaaattc tctatgagcc
     6241 gcaggcttgg ctttttccaa caaatcattt aaaaacaatt tcactttttc cggattttga
     6301 gccattcttt cttctaaaac aaaatgagaa tgagttttgt agcctaacaa atttgctcgt
     6361 tcataacgaa gtttggcaat tttcaacgtg atttctttgt tgtcgaattc attattttga
     6421 aatgcttttt tgccggctgc tattgcgatt tcttttctca attcacggtt ttcaacatac
     6481 gtcacaaatg gtaaataact tggaaaatcc aacgtgaaaa cccaaccatc taatcctttt
     6541 gagttagcta aacttgctgc catttctttc gcaccatcag gtaaaccttt taaatccgcc
     6601 tcgttagtaa tttgtaattg atagttattg gtttctgcca atacattttc accataagtc
     6661 agttttaatt tggctaattc ggtatcaatt tctctgaatc gtactttttt atcttgcgaa
     6721 agtaaagctc cgtttcgggt aaaactttta tattttttat ctaataaagt ggattgttcg
     6781 gtagttaatg ttaatttgtc tttttgatta taaaccgatt ttacacgttt aaataaatct
     6841 tcgttcaacg aaatatcatt gctaaaagca gttaaaagcg gtgaaacttc ctgtgcaatt
     6901 ttttgcattt catcacacgt ttcagcagaa ttcagattaa agaaaatcga tgataatcta
     6961 tctaatgctt cgccagaaa
//
LOCUS       DAOTCW010000118         6852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_8032_length_6852_cov_34.057967, whole genome shotgun sequence.
ACCESSION   DAOTCW010000118 DAOTCW010000000
VERSION     DAOTCW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8032_length_6852_cov_34.057967"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..737
                     /locus_tag="P5335_09185"
     CDS             339..737
                     /locus_tag="P5335_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75091.1"
                     /translation="MQKKGDYSRVNNFLKSILSISGFALFFYCVYRLTLHYKDFLSDN
                     SLKTFLLPVILSILYFPVIAFYAAFHKIDNIFKEINRYQFIDSTRKRKIKLATTIYGN
                     INLDKLTRIRRWDKREIQSNDDIFEYVRQL"
     gene            890..1405
                     /locus_tag="P5335_09190"
     CDS             890..1405
                     /locus_tag="P5335_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75092.1"
                     /translation="MKIINTFVPRLTAFKFQSKDELERNLDLWTDEKYLYAYFEKNKM
                     YLSYFKVKTVKEAVLKTIKQTRLIQKKLYEYIIDETKNLDELFTNFNDEEYHETILSK
                     QKSKQDWLRLYAIKIEPNHYVITGGAIKLVHKMEQSELTKKEKQTIEKCRNYLLDNGV
                     YDADSFYELIL"
     gene            1411..1893
                     /locus_tag="P5335_09195"
     CDS             1411..1893
                     /locus_tag="P5335_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRZ75093.1"
                     /translation="MKTKNLENFKILVSNENSGWLDKFLHYKANKKWLDNSSKVAVNV
                     LEALREKGMSQKDLAEKMKVSAQQINKIVKGKQNLTFETVAKLEDALGITLMDIIGFK
                     STNNIKANVTQIKATQKINTEEIVPNKPFSNDFVKKEGANMSVVYNVLKQPIKYQKAI
                     "
     gene            1893..2321
                     /locus_tag="P5335_09200"
     CDS             1893..2321
                     /locus_tag="P5335_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75094.1"
                     /translation="MADNTIINFNLLKIKTEQFAILEENFNKTEVVNLNTNLSFGLNS
                     EDKVFLVTPKYTFEIKGKPFMTVQISCFFKIEDTTWDSFKKNKQIVFPKDFVAHMAMI
                     TVGTSRGILHTKTEGTVFNDFILPTLNVSKMVGEDVVFDS"
     gene            complement(2626..3885)
                     /locus_tag="P5335_09205"
     CDS             complement(2626..3885)
                     /locus_tag="P5335_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HRZ75095.1"
                     /translation="MYALVDCNNFYASCERVFNPKLNGKPIVVLSNNDGCVIARSNEA
                     KAFGIPMGAPAFEYKKQFEENNIHVFSSNYELYGDLSNRVMKILSTYSPVSEVYSIDE
                     CFLKFDGFDTYFDLQNLGTDMIIKMQKWVGIPISVGFARSKALAKVANKIAKKFPERT
                     NGVYMIDTDEKRIKALKWTKIEDVWGVGRQHTKRLKAINVHNAYQFTQLPDDWIKHNM
                     TIQGLRLKKDLLGIESIQMEEVQDKKNIACTRSFEKMYNDLDNLSERVSTFSSNLGEK
                     LRKQHSHCNLISVFLLSNYFRKDLEQHRAFLTIKTDFPTNSTLELNRIAQKALKMIYK
                     EGVYYKKAGVIVSGITPANNVQLKFFGGENPKHIALMNAIDKTNKKVGGKIKFGQNDL
                     GRKWKMKQEKLSPCYTTRFSDIFTIKL"
     gene            complement(4347..4814)
                     /locus_tag="P5335_09210"
     CDS             complement(4347..4814)
                     /locus_tag="P5335_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75096.1"
                     /translation="MKKTAIIISILITLLVIDYFFYLKKVDNYISESNKLLEYYQSFM
                     INKNNFKPPKNINDITELENWISKTSGKSELIYSNEYNICYDSNTSISYIYVNNSLCK
                     NSLLNHNSVLQNKFGQVEIKKPTFHLYFTEYLLKRNISVLLLKIYPLKESNVP"
     gene            complement(4811..5716)
                     /locus_tag="P5335_09215"
     CDS             complement(4811..5716)
                     /locus_tag="P5335_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRZ75097.1"
                     /translation="MAYKYKYNGKEWQYELGLNMYDMDMRDYDPAIGRWVSQDPVVHF
                     DYSPYSAFDNNPVFWSDPSGADSMQLQDFNGIWHTINSNQYTNIYSASDNDSNDNDDW
                     VYNTSSQTYQWNENATSKGTTPKGFEYVGKTKSDIDRHYKKKHNFKSFFRIRPNIDFE
                     SKYSYDIKSIAMQVINAYKNRKYIPLTPQQIADNINRAPDDRVEPRMSSFSININQTY
                     PTFNRDITLNLNGTLVTGSLGYSHTVESVNFISSITEGKYVPQSIMSGHNNYSLLFGN
                     SQKNNVFIIQFNSSEYQKIINYLQQ"
     gene            complement(5885..6190)
                     /locus_tag="P5335_09220"
     CDS             complement(5885..6190)
                     /locus_tag="P5335_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRZ75098.1"
                     /translation="MKTKNNNIKTLDHFKDKHYGKVGTAKRDELETGYDNFRIGAMIH
                     DARLQKGLTQEELAEKVGTTKSYISKIENNVKEARFSTLQKIVELGLGGHLELSIKI"
     gene            complement(6174..6509)
                     /locus_tag="P5335_09225"
     CDS             complement(6174..6509)
                     /locus_tag="P5335_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRZ75099.1"
                     /translation="MKQRTVIFYKDYFEEFFEKQRDKVKNKIIWTFQLIEELERIPET
                     YLKHIENTDGLFEIRVQQGSDIFRIFCFFDEGQLVVLANGFQKKTQKTPKNEIERAIK
                     IKNEYENEK"
BASE COUNT         2386 a         1032 c          933 g         2501 t
ORIGIN      
        1 taaaagcatt ttttgcaaag caattaatcc ctcattattt agtaattata ggctatttta
       61 ctcttgtatt atatttatta aaacaaacaa ttttatggga ggaaaatcta ttgaaggatt
      121 ttattatttg gtgtgtagct tttggttttt actcgttttt tattactaac aaaatccaca
      181 ctaataaaga tttatttaga caattttgga aaatattttc gatcacaatt tttgttgatt
      241 tttttcttaa ttattttact ttcagcttgg tttgggaaat aattttaatc ccggttctat
      301 cctttattgc gatacttcaa ttcttcacag aatatcatat gcagaaaaaa ggtgactatt
      361 caagagtaaa taacttttta aaatctattc tatctatttc aggatttgca ctttttttct
      421 attgtgttta taggttaaca ttacattaca aagatttctt gtctgacaat tcattaaaaa
      481 catttttgtt gccggttatt ctttccatac tttatttccc agtcattgct ttttatgcag
      541 cctttcataa aattgataat atttttaaag aaataaaccg ctatcaattt attgacagca
      601 cccgaaaaag aaaaattaaa ctggccacta ccatttatgg aaacataaac cttgataagt
      661 tgaccagaat tagaaggtgg gataaaaggg agattcaatc taatgatgat atatttgagt
      721 atgtaagaca actgtaaatt taactgtcct ttattcgaaa taaagttcat caaaatcatt
      781 tgataaatat gtttactgaa atttgctttt gtttttgttg ataactaaaa tttgacttaa
      841 ctcaaaaatc atataatttt gctaaaaaat aaacctataa gttgatttaa tgaaaataat
      901 caatacattt gtaccacgat taaccgcttt taaatttcaa tctaaagatg aattagagag
      961 aaatttggat ttgtggacag atgaaaagta cttatacgcc tattttgaaa aaaataaaat
     1021 gtacctatct tatttcaaag taaaaactgt aaaagaagcg gtattaaaaa cgatcaaaca
     1081 aacacgtctt attcaaaaaa agctttatga atacatcatt gacgaaacaa aaaaccttga
     1141 tgaattgttt acaaatttta atgacgaaga ataccatgaa acaatcttat caaaacaaaa
     1201 atcaaaacag gattggctta gactttatgc cataaaaatt gaacctaacc attatgtaat
     1261 aacaggcggt gcaattaagc ttgttcacaa gatggagcaa agtgaactca caaaaaaaga
     1321 aaaacaaaca attgaaaaat gtagaaatta tttgcttgat aacggtgttt atgatgcaga
     1381 ttctttctat gaattgatcc tctgactaaa atgaaaacta aaaatttaga gaattttaaa
     1441 attctggttt caaatgaaaa ctcaggttgg ctagataaat ttttacatta taaagccaat
     1501 aagaaatggc ttgataattc ttcaaaagta gctgttaatg tacttgaagc actgagagaa
     1561 aaaggaatgt cacaaaaaga tttagccgaa aaaatgaaag tttcagctca acaaataaat
     1621 aaaattgtaa aaggcaaaca aaatctaacc tttgaaacag ttgcaaaact tgaagatgct
     1681 ttaggtatta ctttaatgga tattattggc tttaaatcaa ctaataacat aaaagcaaat
     1741 gtaactcaaa tcaaagcaac tcaaaaaata aatactgagg aaatagttcc aaataagcct
     1801 ttctcaaacg acttcgttaa gaaagaagga gcaaacatga gtgtggttta taatgtttta
     1861 aaacaaccaa taaaatatca aaaagcgata taatggctga taatacaata ataaatttta
     1921 atcttcttaa aattaaaact gaacagtttg caattttaga agaaaacttt aataaaactg
     1981 aagtcgtaaa tctaaacaca aatttgtctt ttggtttaaa ctcagaagat aaagtctttt
     2041 tagttactcc aaaatatact tttgagatta aaggcaagcc atttatgaca gttcaaataa
     2101 gctgtttttt taaaatagaa gacacaactt gggacagttt taagaaaaac aaacaaattg
     2161 tttttccaaa agattttgtt gctcacatgg ctatgattac tgttggaact tctaggggaa
     2221 ttcttcacac taaaacagaa ggaacagttt ttaatgattt tatacttccg acactaaatg
     2281 tttctaaaat ggttggagaa gatgtagtgt ttgatagtta attaaattat ttagttttag
     2341 aatttataaa tgcaaaaacc tatacaattt ctgggtttat ttctttacaa atttaaataa
     2401 tatgttccat acaaaacata tatcttaagg ctaaacatct ctccagtata aggaaatgaa
     2461 aaattataaa tcttatttga attgtctaac catgccaatt catctctttt tttaagaata
     2521 attggcattc attttttatg gttatgaaaa tatttcatct tagctataaa tcgtttctca
     2581 acctttttaa gaggttcact ttgagccatg aaaaaacaca taaatctaga gttttattgt
     2641 aaatatatca gaaaatcggg tagtataaca aggcgataat ttttcttgct tcatcttcca
     2701 ttttctaccc aaatcatttt gcccaaattt gatttttcct cctacttttt tatttgtttt
     2761 atctattgca ttcattaaag caatatgttt ggggttttct ccaccaaaaa atttcaattg
     2821 cacattattt gcaggagtta ttcctgaaac tattacaccc gcttttttat aataaacacc
     2881 ttctttgtaa atcatcttta aagccttttg agcaattcta ttgagttcta aagtagaatt
     2941 tgtcggaaag tcggttttaa tcgttaaaaa cgctctatgt tgttctaaat cttttctgaa
     3001 atagttagat aaaagaaata cactaattaa attgcagtgc gaatgctgtt ttctaagttt
     3061 ctcccctaga ttggaagaaa aagtactcac tcgctcactt aaattatcta agtcattata
     3121 cattttctca aaactacggg tgcatgcaat attcttttta tcctgcacct cctccatttg
     3181 aatcgattca attcccaata aatccttttt aagtcttaac ccctgaatag tcatgttgtg
     3241 tttgatccaa tcatccggca attgtgtaaa ctgataagca ttatgcacat taatagcttt
     3301 cagtcgtttt gtatgttgtc tacccacacc ccaaacatct tcaatttttg tccacttgag
     3361 agcttttatt cttttttcat cggtatcaat catatacact ccattagttc tttccggaaa
     3421 tttctttgca attttgtttg caacttttgc aagtgccttt gacctggcaa aacctactga
     3481 aattggtatt cccacccatt tttgcatttt tattatcatg tctgtaccta aattttgcaa
     3541 atcaaaataa gtatcaaatc catcaaattt taagaaacat tcatcaatag aatacacctc
     3601 acttaccggt gaataagtag ataaaatttt catcactcga ttcgacaaat caccatataa
     3661 ttcataattg gatgaaaaaa catgaatatt gttttcttca aattgctttt tatattcaaa
     3721 tgcaggagca cccatcggaa taccaaatgc ctttgcttca ttacttcttg caataacaca
     3781 accatcattg ttcgacaaaa caacaattgg tttcccgtta agtttagggt taaaaaccct
     3841 ctcacaactt gcgtaaaagt tgttgcagtc gactaatgcg tacataaatt tgtgatgaag
     3901 agtttacaaa tttatttaaa attaagaggg gattttgggt tgttgataaa taaagttttg
     3961 ggtgtgaatg attggatagg actatatttt aatctgtttc atcgtcacta attattttgc
     4021 aacaattaag aaaatgaata attatcccaa aaaataaatt atcaaaacac acagcaaaac
     4081 agaataattc ttagaataac aaatgatttt gcggtttagt cgtttcaagt tattgtgtaa
     4141 agtgaggttg ttactttcaa tttacaattt ggacaaattc ccgtatcatt aactttgaaa
     4201 cacgtggtgt catttttctt cattacattc gctttaagct attttctaaa atataaaagg
     4261 caaactatta agcttgccct ttgggtttta attttttaag gattgattta catatggtta
     4321 aattgtgggt tttggttcat cactaattat ggaacattac tctccttcaa agggtaaatc
     4381 tttaacaata aaaccgaaat gtttctttta agtaaatact ctgtaaagta caaatgaaat
     4441 gttggttttt ttatttcaac ttgaccaaat ttattttgta aaacagaatt atgatttaac
     4501 aatgaatttt tacataaaga attattcaca taaatatatg aaatgcttgt gtttgaatca
     4561 tagcaaatat tatactcatt agagtaaatt aattctgatt tcccacttgt tttagaaatc
     4621 caattttcta attctgtaat atcgtttata ttttttggtg gtttaaaatt atttttatta
     4681 atcataaatg attgataata ttccaacaat ttatttgatt ctgaaatata attatcgact
     4741 ttttttaaat aaaaaaaata atcgataact aataaagtaa ttaatattga aataattatt
     4801 gctgtctttt tcattgttgt agatagttaa taattttttg atattctgaa ctgttaaatt
     4861 gtattataaa aacattattt ttttggctat taccaaataa taaagaatag ttattatgac
     4921 cagacattat actttgtggc acatatttac cttccgttat acttgaaata aaatttacac
     4981 tttctacagt atgtgaataa cctaatgaac cagttactaa agttccattt aaattaagcg
     5041 ttatatccct attaaatgtt ggataagttt gatttatatt aatcgaaaaa ctagacattc
     5101 ttggttcaac tctgtcatct ggtgctctgt ttatgttatc ggcaatttgt tgtggggtaa
     5161 gaggaatata ctttctgttt ttataagcat ttataacctg catagctatt gattttatat
     5221 catatgagta ttttgactca aaatctatat ttggtctaat acggaaaaaa cttttaaaat
     5281 tatgtttttt cttgtagtgt ctgtctatat cagatttggt tttccctacg tattcaaaac
     5341 cttttggagt agtgccctta ctagttgcat tttcattcca ttgatatgtt tgtgaacttg
     5401 tattatagac ccaatcatca ttatcattag aatcattatc ggaagcacta taaatattag
     5461 tatattgatt tgaatttata gtgtgccaga ttccattaaa atcttgtagt tgcatagaat
     5521 ccgctccact agggtcagac caaaacacag gattattgtc aaaggcacta taaggtgaat
     5581 aatcaaaatg aacaactgga tcttgagaaa cccaacgacc aatggcagga tcataatctc
     5641 gcatgtccat gtcatacatg ttgagtccca actcgtattg ccactccttc ccattgtatt
     5701 tatacttata cgccattgcg aaataccatc ataccgagaa cgtccattca acattatctt
     5761 cttggatacg aaagtagata aaaatctgta aatgttaaaa taattgaata ttaagttttc
     5821 aatttagata ggcacaaaaa aaccctcaaa ttgagagcct taatttttat tattttggtt
     5881 ttacctatat cttaattgac aattccaaat gtccacctaa accaagttcc actatctttt
     5941 gaagagttga gaatcttgct tcttttacat tattctcaat tttagaaata taagatttag
     6001 tagttccgac tttttctgct agttcttcct gtgtcaaacc tttttgaagt ctggcatcgt
     6061 gaatcattgc accaattcta aagttatcat aaccagtttc caattcatca cgtttagctg
     6121 ttcccacctt accataatgt ttgtctttga aatggtctaa tgttttgata ttattatttt
     6181 tcgttttcat attcgttttt tattttgatt gctctttcaa tttcattctt tggtgtcttc
     6241 tgcgttttct tttggaatcc atttgctaaa actaccaatt gtccttcatc aaaaaaacaa
     6301 aagattctaa agatgtcgct accttgttgg actcggattt caaataatcc atctgtattt
     6361 tcaatatgtt taagatacgt ttcgggaatt ctttctaatt cttcaattaa ttgaaatgtc
     6421 cagataattt tattttttac tttatctcgt tgcttttcaa agaattcttc aaagtaatct
     6481 ttataaaaaa tgactgttct ttgtttcatg ctacaaatat acaaaagttg caccaaagta
     6541 caacttttcg gttaaatatt ttcatttgag tggaaaatat ttgcatatcc agatataatc
     6601 agttaattga atacagaaaa ggtaatgaaa taggttttaa agattgggat aaacgatact
     6661 gttaagagat attaaaattt ctttaaacct gtcgaattaa aaagaaaaaa gtgttctgtt
     6721 tttcggcaaa atatcggcaa aaagaaaaaa gaaaaatccc taacaagttg aatttcaata
     6781 tgttagggat ttttaaagta cccggagtgg gaatcgaacc cacacaccta agtacacgag
     6841 tttgagtcgt gc
//
LOCUS       DAOTCW010000119         6845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_8046_length_6845_cov_31.694698, whole genome shotgun sequence.
ACCESSION   DAOTCW010000119 DAOTCW010000000
VERSION     DAOTCW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8046_length_6845_cov_31.694698"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..772)
                     /locus_tag="P5335_09230"
     CDS             complement(257..772)
                     /locus_tag="P5335_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75100.1"
                     /translation="MFQYCHECINEYINAPSWITPIAQSITALVAIFALSVAWKNLRG
                     LKRTQSLQAQMNLISLENEVRKNYLMLKIANQKYSEETQISNSKNILMLSIEKTNAFE
                     LYVSTADKLAALIASEFLTGQFEKRNWKDEYLAIFKKVKEYHQGEDSIIPGKDQMINN
                     INKLLIEWSKS"
     gene            complement(775..1038)
                     /locus_tag="P5335_09235"
     CDS             complement(775..1038)
                     /locus_tag="P5335_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75101.1"
                     /translation="MLLDFEQFTRAAEEVERIIALPTNADLMSEINNSSSTIPVEKRR
                     EFYRHMNSLFLNVLLNRLDGLGAGAEQRAIEARRIIVNIINTL"
     gene            complement(1051..5997)
                     /locus_tag="P5335_09240"
     CDS             complement(1051..5997)
                     /locus_tag="P5335_09240"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Helicase associated domain protein"
                     /protein_id="HRZ75102.1"
                     /translation="MNKENQILIELLKGVENWTELKVNLEKYNTAQTETTSKKTLAGK
                     IFEVFTKYYLQTEPKKTELYKNVWLYDEISIDIKEQLRLPAIDHGIDLLLQDFDNNFH
                     AVQCKFKNDETKSLSWSGDKIANVFALGTNCHKVIVFTNASDTTSVAKAFEQKFEQIA
                     FDELNSIEPEIFKNILELALGNRPKELKKHTPKEHQEIAINKVVEHFATNDRGQLILP
                     CGAGKTVTALWIKERIKSNTTLVLVPSLALLKQIKNDWARHKTNPYAYLCVCSEKDID
                     KDQEDAIRLHTYEIGGPVTTDFEEVKLFLEKESERIIFSTYQSIEVIVSAANRIKDFS
                     FDLVICDEAHRTAGSRDKNTFTLVHNNQKIPALKRLYMTATPKIVSKKLKRKLGEDYE
                     LFCDMSNPRIFGNEAYHMTFGDAISKNILVDYKIIGIGVSDKQVKKYIDERKYLGNYT
                     AEDLANNFALELVMNNYNAFHSISFHSKVKFAEAFATRHKDFFAEVFSKSVNGNQTTT
                     YRSKVLQEFKESPKGLVSNARCLTEGVDVPSIDLIYFCDPKTSVIDIVQASGRALRLD
                     PSGKKEWGYIVVPIFHHEDQDVETEIKKKPIFEHLIQVIRSLCDHDERLLAEINEIAF
                     KKGKRSNSRIRIDFPDSKIEKIIKLEGIEEKVRDVLFDEIIEKTSSNWELRFLEIKDY
                     IEKNGHSTISKNNKDYIELYYWVGNQRKYYYEDKLEPSKIIRLNEIGFDWKGQNRREI
                     TDLDEIWKESYRKLEKYYEENNNSDVPARYALDKSLGTWCVSQRVKYDEEKLADWQIE
                     LLEKLKFNWDPKNVFDKRYSELLEYKKKFGNVKVPTQGSKEYKDLGRWVNRLRVIYKS
                     GNVDEQGNVRSSGFGHIKAHQLKLLNEIGFLWNAVKGDWNDRFAELKEYFDKNGHSNV
                     KQAEDFSLYQWCYKQKKNKESLTTDKIEKLNSIEFNFEFQPQTGSDAGLVVWSERLSE
                     LSDYFESFGDYNIPRNDPEYKSLLIWLRHQRSLQLQGNLSDEKLSWLEDIGFSLDEFF
                     STDDVENWNINLHKLKDYFENTGSFYIALNDEVNKSLLSWIRYQKSLFKQEKLSDDKI
                     EKLKSIGYSFEINYRGKGVRTKKSEIDNEWLEMLNNLKAYYDKNKTFLIPNKDNTNQT
                     LRNWLRYQKNLYNTGMLGAEKENHLKGIGYSFSLNFRGKSLNSISNELNPVEKDDKPS
                     TWEIKFEEFKKYYLVNQTFFIPSDDKELQPLLSWVRYQRILYKEDKLEKNKLEIFNEL
                     GYSFELDFRGRKGNDENSIKEDWFTRLSQLHEYYKETGTFFIKGNDKDNSDLLQWLRY
                     QRILRKQGKLTTEQLEALTNIGYSFEVKYYGQGREKKPKTENQKPENERNSWDSNYLK
                     LLNYKMKVGHCNVPRDFKDDKPLANFVVRQRAYYKQGKLSNDQINKLEFLGFEWEVDR
                     TKVWMKAWNNKYSQLLSIFKKTNSSHITSSYEDNSLYTWTLQQRMKKRNNELSDEQIK
                     LLDDIAFNWNPEAASGSPDDERWFEMLEQLSEYKNKFGDCNVSQLNKDYKKLGRWVND
                     QRLNYSRGKLFEHRKELLEELDFIWNTKEHEFDLKVKMLQEFHMKNGHFDVKQSDKEY
                     GGLYDWLHRLIKKGTTETRKKKLESIGYDTSIIKTIVN"
BASE COUNT         1922 a         1275 c          969 g         2679 t
ORIGIN      
        1 atcactggct tggtttggtc cgaaatgact ggcagggtat agtccgaaat agtcagtctt
       61 tatacagttt tagtgtcatt gtcattttac tttctttctt ttttgacggc ttgtgtgtct
      121 gcgtttttta tttttttaag tggaggcaca ttttttaaaa caatttctct cggtgcattg
      181 gctttgcaag ctctttgaca aagctttggt tgcagcgttg gcacgagggg ctttggcaat
      241 gtgcttgcaa aataggctat gattttgacc attcaattaa tagtttattt atgttgttta
      301 tcatttggtc tttgccaggt attattgaat cttcaccttg atgatattcc tttactttct
      361 taaatatagc tagatattca tctttccaat tacgtttttc aaattgacca gtaagaaatt
      421 ctgatgctat taaagctgct aacttgtcag cggttgaaac atataattca aaagcattcg
      481 ttttttcaat acttaacatt aatatatttt tcgaatttga aatttgagtt tcctcagaat
      541 atttttgatt agctatttta agcattaaat agtttttccg aacttcattc tcaagtgaaa
      601 taaggttcat ttgtgcttgt aatgattgag ttcttttaag acctcttaag tttttccaag
      661 ccacagataa cgcaaatata gcaaccaatg cggttattga ttgagcaata ggagtaatcc
      721 atgacggagc atttatatac tcattaatac attcgtgaca atattgaaac atagctataa
      781 tgtattaatt atgtttacaa taattctcct tgcttctata gctctctgtt ctgcaccagc
      841 gccaagtcca tctaatctat tcaacaaaac atttaaaaat aaagagttca tgtgacgata
      901 aaattctctc ctcttttcca ctggtattgt cgaacttgaa ttattaattt ctgacattaa
      961 atcagcattg gtaggaagtg cgataattcg ttcaacttcc tctgcggctc tagtgaattg
     1021 ttcaaaatct aataacatct aatatttttt ttaattaaca attgtcttta ttattgaagt
     1081 atcgtaacca atactttcaa gtttcttttt tctcgtttct gttgttccct ttttaatcaa
     1141 cctatgaagc caatcataca aaccgccata ttccttatca ctttgcttta catcaaaatg
     1201 tccatttttc atatggaatt cttgaagcat tttaaccttc aaatcaaact catgctcctt
     1261 agtattccaa atgaagtcca attcttcaag tagctccttc ctatgttcaa acaatttgcc
     1321 tctagaataa tttagtcttt ggtcatttac ccatctgcca agttttttgt aatctttatt
     1381 caattgagat acattgcaat ctccaaattt atttttgtat tcagataatt gctcaagcat
     1441 ttcaaaccat ctttcgtcat caggtgagcc acttgcagct tcaggattcc aattaaatgc
     1501 aatatcatct aataacttaa tttgttcatc tgataattca ttattacgtt ttttcattcg
     1561 ctgttgcaga gtccatgtat aaagcgaatt atcttcatat gaactcgtta tatgcgaact
     1621 gtttgttttt ttaaatattg ataacaattg tgagtattta ttattccatg ctttcatcca
     1681 tacctttgtc ctgtcaactt cccactcaaa accaagaaat tccaatttgt ttatttggtc
     1741 attgcttaat ttcccttgct tgtaatatgc tctttgtcgc acgacaaagt tcgctaaagg
     1801 cttatcatct ttaaaatctc ttggtacatt acaatgcccg actttcattt tatagttcaa
     1861 taactttaaa tagtttgagt cccaagaatt acgctcattt tcaggttttt gattttctgt
     1921 ttttggtttt ttctctcgac cttgtccata atattttact tcaaaagaat acccgatatt
     1981 tgttaatgct tctagttgtt cagtagtcaa cttaccttgc ttcctcagga ttcgctggta
     2041 tctcaaccat tgtaataggt ctgaattatc tttatcattt cccttaataa aaaatgttcc
     2101 tgtttcttta taatattcgt gcaactgtga taatcttgta aaccaatctt cttttattga
     2161 attttcatca tttcctttcc tgcctcgaaa atcaagttca aaagaatatc ctaattcatt
     2221 aaatatttca agtttatttt tttcaagttt atcttctttg tacaaaatcc gttgatatct
     2281 tacccaactt aatagtggtt gtaattcttt atcatccgaa ggaatgaaaa aggtttggtt
     2341 gaccaaataa tactttttga attcttcaaa tttaatttcc caagttgagg gtttatcatc
     2401 tttctcaact ggattgagtt cgtttgaaat agaatttaag gatttacccc gaaaatttaa
     2461 actaaaagaa tacccaatac cctttaagtg attttccttt tcagcaccta acattccagt
     2521 attgtataga tttttctgat accgtaacca gtttctaagg gtttgatttg tattatcttt
     2581 atttgggatt aaaaatgttt tatttttatc atagtaagct tttagattgt tcaacatttc
     2641 aagccactca ttatcaattt cacttttttt tgttctaact cctttacctc tgtaattaat
     2701 ttcaaatgaa tatccaatac tttttagttt ttctattttg tcgtcactca atttttcttg
     2761 tttgaataag gatttctgat agcgaatcca acttaaaagg cttttattta cctcatcatt
     2821 cagagctata taaaaactgc ctgtattttc aaaataatcc tttaatttat gaagattgat
     2881 attccaattt tcaacatcat cagtactgaa aaattcgtct aatgaaaatc caatatcttc
     2941 aagccatgaa agtttttcat ccgataaatt accttgtaat tgcaaacttc tttggtgtct
     3001 gagccaaatt agtaaacttt tatattcagg gtcgtttctt ggaatattat aatctccaaa
     3061 tgattcaaaa taatcggaca attcagataa tctttcagac caaacaacaa gtcctgcatc
     3121 actgccagtt tgaggttgaa attcaaaatt aaattcaatt gaatttaatt tttcaatttt
     3181 atcggtcgtt aagctttctt tattcttttt ttgtttataa caccattgat acaaagaaaa
     3241 gtcttcggct tgttttacat ttgaatgtcc attcttgtca aagtattctt ttaattctgc
     3301 aaatctatcg ttccaatcac cttttacggc attccataaa aaccctattt catttagtaa
     3361 tttaagttga tgtgctttaa tatgaccaaa cccacttgaa cggacattac cttgttcatc
     3421 aacgttcccg cttttataaa taactcttaa cctatttacc cacctgccaa ggtctttata
     3481 ttctttactg ccttgtgttg ggacttttac attgccgaac ttttttttgt attctaataa
     3541 ttcactgtat ctcttatcaa atacattttt ggggtcccaa ttaaatttta atttttcaag
     3601 taattcgatt tgccaatcag ctagtttttc ttcatcatat ttgactcttt gcgaaacaca
     3661 ccaagtacct aatgatttat caagggcata acgggctggc acatctgaat tattattctc
     3721 ttcataatat ttttcaagtt tacgatagct ctctttccaa atttcatcca aatcagtgat
     3781 ttctcttctg ttttgacctt tccaatcaaa gcctatttca ttgagcctta taatctttga
     3841 aggttctaat ttatcctcgt agtaatattt tcgttggtta ccaacccaat agtataattc
     3901 aatgtaatct ttattatttt ttgaaattgt ggaatgacca tttttctcaa tataatcttt
     3961 aatttcaaga aatcttagtt cccaatttga gcttgttttt tcaattattt catcaaataa
     4021 aacatctcga actttttctt caatgccttc aagtttgatt attttttcaa ttttagaatc
     4081 aggaaaatct attctaattc gcgaattact ccgctttcct tttttaaaag ctatttcatt
     4141 aatttcggca aggagtcttt cgtcatggtc gcaaagcgac ctaattacct gaattaagtg
     4201 ttcaaaaatt ggcttctttt taatttctgt ttcaacgtct tggtcttcgt gatggaaaat
     4261 tggtactaca atgtatcccc attctttttt accgcttggg tcaagtctta atgctctacc
     4321 agatgcttga acaatatcaa tgacagatgt ttttgggtca cagaaataaa ttaaatcaat
     4381 cgaaggaaca tcaactccct cggttaaaca tcttgcattt gaaacaagac cttttggact
     4441 ttctttaaat tcttgtagta cttttgaacg ataagttgtg gtttgatttc cgttgacact
     4501 tttagaaaat acttctgcaa aaaaatcttt atgtctagtg gcaaacgctt ctgcaaattt
     4561 aacttttgaa tgaaatgaaa ttgaatgaaa tgcattgtag ttattcatta ccaattctaa
     4621 agcgaagtta tttgctaaat cttcggcagt gtagtttcca agatattttc gttcatctat
     4681 atatttttta acttgtttat cagaaacacc aatgccaata attttataat caactaaaat
     4741 attctttgat attgcatctc caaaagtcat atgatacgct tcattaccaa aaattcttgg
     4801 attactcata tcacaaaaca attcataatc ttccccaagt ttacgtttca atttctttga
     4861 aacaattttg ggagtagctg tcatatacaa cctctttaat gcaggtattt tctgattatt
     4921 gtgaactaat gtaaatgtat ttttatctct actgccggcg gttctgtgag cttcgtcaca
     4981 aataaccaaa tcaaaagaaa aatccttaat tctattagca gcacttacaa ttacttcaat
     5041 tgattgatat gttgaaaata taattctctc actctccttt tctaggaata gttttacttc
     5101 ctcgaaatca gttgtgactg gaccacctat ttcataagta tgcaatcgaa tagcatcttc
     5161 ttggtcttta tcgatatctt tttcggaaca aacacataaa tacgcataag ggttggtttt
     5221 atgtcttgcc caatcgtttt tgatttgctt tagcaaagca agtgacggaa ctaaaaccag
     5281 cgttgtattt gattttatcc tttcttttat ccatagggca gttactgttt ttcctgcacc
     5341 gcaaggtaaa attagctgac ctctatcatt ggttgcaaag tgttctacta ctttattaat
     5401 tgctatttct tgatgctctt ttggagtatg tttttttaac tcttttggtc tattgcctaa
     5461 ggctaattca agaatatttt taaatatttc tggttcaata gaatttaatt catcaaaagc
     5521 tatttgttca aatttttgct caaatgcttt tgcaacactt gttgtatcag aagcattcgt
     5581 aaaaacaatt actttatggc aatttgtgcc aagtgcaaat acatttgcta ttttatcacc
     5641 tgaccagctt aaactttttg tttcgtcatt tttaaattta cactgaaccg catggaaatt
     5701 attatcaaag tcttgcagta gtaaatcaat tccatggtca attgcaggta gtcttaattg
     5761 ttcctttata tctatagaaa tttcgtcata caaccataca ttcttataaa gttcagtctt
     5821 ttttggttct gtttgcaagt aatattttgt aaatacttcg aatattttcc cagctaaagt
     5881 cttctttgaa gtagtttcgg tctgagctgt attatacttt tcaagattaa cttttaattc
     5941 agtccaattc tcgaccccct ttaataattc aattaatatt tggttttctt tattcatagc
     6001 taattcacgt gggttggcaa aattgcactc tttgacaaag ctttggtttt agcgttggct
     6061 tgtggggctt tggcaatgtg tgcaatgtgg gtcggcaatt gttttaaaaa atgtgcggtg
     6121 ggcaaaaaat aaaaaacttc gggtcggctt gagtgtcggc tctcttttct tgtcaatatt
     6181 tcgtttctct gtcctttcac aatggtttac ttttcaattt tctgtctgtc ggttctgttt
     6241 tctacacttg gcggtaacgc catgtgcaga agccggcccc ggtacacacc tcactatttt
     6301 cccagaggcc ggtgggctgg catttgcaca acgttatggg ctggccactt tcataagtac
     6361 ttttttaatg cttccaaggt tataatgctc ataaacaacc acctgctctt ccggactcca
     6421 actttttttt atcatagata cggtagaatc aaggccaaat gtcctgagct tgcagatccg
     6481 attgtctttt aattatccat ttttttctat tatgcttgcc cataacgcca tgtgcagaag
     6541 ccggccccgg tacacacctc actattttcc cagaggccgg tgggctggca tttgcacaac
     6601 gttatgggct ggcctcattc ataagtactt ttttaatgct tccaaggtta taatgctcat
     6661 aaacaaccac ctgctcttcc ggactccaac ttttttttat catagatacg gtagaatcat
     6721 ggccaaatgt cctgagctcg cagatccgat tgtcttttga tcatccattt ttttctatta
     6781 tgcttgccca taacgattac gtatatgaaa cgtttggcat ttcgaatcac tttcctgtca
     6841 agtta
//
LOCUS       DAOTCW010000120         6810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_8120_length_6810_cov_47.361214, whole genome shotgun sequence.
ACCESSION   DAOTCW010000120 DAOTCW010000000
VERSION     DAOTCW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8120_length_6810_cov_47.361214"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="P5335_09245"
     CDS             complement(<1..197)
                     /locus_tag="P5335_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRZ75103.1"
                     /translation="MHFLSEDLEDYVAFHSQNEPELLAQLNKETYQKILLPRMLSGHF
                     QGRVLSMLSKLIRPISILEIGT"
     gene            270..1322
                     /gene="rlmN"
                     /locus_tag="P5335_09250"
     CDS             270..1322
                     /gene="rlmN"
                     /locus_tag="P5335_09250"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRZ75104.1"
                     /translation="MNTTKKDIRALTKEQLREFFVSQGDKAFRGNQIYEWLWSKKVYQ
                     FDDMTNISKETRQMLEDNFVINHIKVDQMQRSEDGTVKNAVRLYDGLVVESVLIPTAT
                     RTTACVSSQVGCSLDCNFCATARLKRMRNLNPDEIYDQVVAIDNESRLYFDRPLSNIV
                     FMGMGEPLMNYNNVLKAIEMITSPEGLGMSPKRITLSTSGIPKMIKKMADEEVKFKLA
                     VSLHSAIDEIRAEIMPFSKSFPLKELRESLEYWYQKTKSKITYEYVVWKGINDDKKSI
                     DALVKFCKYVPCKVNLIEYNPIDDGMFQQASDDALEHYVSALDKIGVIAKVRRSRGKD
                     IDAACGQLANKNEAIS"
     gene            1370..>2279
                     /locus_tag="P5335_09255"
     CDS             1370..>2279
                     /locus_tag="P5335_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRZ75105.1"
                     /translation="MKITEQIKQPILAEMELFEKKFYESMSSKVALLNRITYYIVNRK
                     GKQMRPMFVFLTAKMVSDGIVNERTFRGASVIELIHTATLVHDDVVDDSNRRRGFFSI
                     NALWKNKIAVLVGDFLLSKGLLLSIDNNDFDLLKIISVAVREMSEGELLQIEKARRLD
                     ITEDIYYEIIRQKTATLIAACCSLGACSVLPENKEVVERMRKFGELIGMAFQIKDDLF
                     DYTEEAIGKPTGIDIKEQKMTLPLIYTLNTCSSKEKSWLINSIKNHNLDKKRVKEVIA
                     FVKSSNGLTYAEQKMIQFQQEALLLLE"
     assembly_gap    2280..2379
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2385..2945
                     /locus_tag="P5335_09260"
     CDS             2385..2945
                     /locus_tag="P5335_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRZ75106.1"
                     /translation="MKVINLHQDEQTTIRMAIEQNRFAQQQIYAKFSPKMLSVCRQYI
                     KDLQQAEDVMLTSFMKVFTNLKKFEHKGSFEGWIRRIMINECVSFIRVQKQVKFIEEE
                     TFFEERHNDIESKFSVDDIQFLIDGLPDGYRMIFNLYAIEGYKHQEIAVMLGINEGTS
                     KSQLSHARKLLQEQITKLKNYDNGTE"
     gene            2932..3615
                     /locus_tag="P5335_09265"
     CDS             2932..3615
                     /locus_tag="P5335_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75107.1"
                     /translation="MEPNKLENKMREKLNKRTIQPSANAWDRLDAMLSVTEQKPKRNF
                     KWLSIAAVFVGFTLIGIFILNKEKSIENVIQSNPIVLENETEKVENLNEIGKENTISS
                     EENQEEVVLINQSKKKMDEQPIAINPKKDFLLDNYSKTEEVIVENQPKEAIYKYVNAK
                     SLLAEIETGEKLETPTLSKKPTVKVDANALLSSAEKEVEETFRDKVIQSINKKYNSVK
                     STLANRNNE"
     gene            3652..4764
                     /locus_tag="P5335_09270"
     CDS             3652..4764
                     /locus_tag="P5335_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75108.1"
                     /translation="MQKIIFYVAVLLCTFLTQIYGQETFEQQAKVIANSISSITTEEK
                     AALKAEVEEINKLLESGTITATQADEKKIKAAEEKAKNIERRVATEQAKLDILVKDVV
                     DGKVNYSKEKEKWGTTIILGTNSNDSIGENHTEISLGSMKVYKGETEKIRRNSKRTTS
                     QFVFAFGLNNLITDGDSNSLENSDFKTWGSNFFEWGFTFNTRILKDNNLLHFKYGLSL
                     MYNNLRPTDNRYFVKDGDVTQLQTYDRDLNENKFRNSMVVLPVHLEFDFTPKKVGDDG
                     SFKFRTHKSVRLGVGGFGGLNYQSTQKLRYEEDDIRIKNKQKGDFNVSTFVYGLSGYL
                     GYGSTSLYVKYNLNSLFKDNPIDQNNISLGLRFDFN"
     gene            4862..>6810
                     /gene="dnaG"
                     /locus_tag="P5335_09275"
     CDS             4862..>6810
                     /gene="dnaG"
                     /locus_tag="P5335_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRZ75109.1"
                     /translation="MITQNTIDLVFETARVEEVIGDFVQLKRAGSNMKGLSPFSNEKS
                     PSFMVSPVKQIWKDFSTGKGGNSVTFLMEHEHFSFPEAIRYLAKKYNIEIEETVSSDE
                     DKLVANEKESMYLVSEFASKYFQHVLWEEEEGKAIGLSYFKERGFSNETIKKFNLGYS
                     PNTWDALTKEALGKAYKLEFLEKTGLTIVGEDKQFDRFKGRVMFPIQSMSGRVLGFGG
                     RILTNDKKAAKYLNSPESEIYHKSNVLYGIFHAKQSIAKLDNCFLVEGYTDVIQMHQA
                     GIENVVSSSGTALTPNQIRLINRLTKNITVLFDGDAAGLRASIRGIDLILEEGMNVKV
                     CTFPDGDDPDSFAKKTPYEELVKYLEENSKDFIQFKASLLMKDAKNDPIKKADLIRDM
                     VTSISKIPDRIKKEIYIQECARIMDISEEVLFSTLAQMAQKDLSEANKKFKQEQKVFE
                     VVKNETTATVSKVDIISLLEKTILEILLLYGHHEVEFENVFIDYDENGKEIEINDKVN
                     QKIYERIYLSLQEDEIELANPIFNAIYVDLVSQYHQNETLDKNHYLNNLLPEHAEIVT
                     DIIMNDEKYQLHGWLDKKQVFVKEKDDLEILSRLVSETIVTFREYLINKLNRELMLQM
                     QEEIPELSPEEIITMINDYNKLKVS"
BASE COUNT         2437 a          978 c         1276 g         2019 t
ORIGIN      
        1 gttccgattt ctaaaataga tattggccga attaattttg ataacatact taaaactctt
       61 ccctgaaaat ggccgctcaa catgcgtggc agtaaaattt tctgataagt ttctttgttt
      121 agttgggcca ataattcagg ctcattttgc gaatgaaagg caacgtaatc ttctaaatct
      181 tcagaaagga aatgcattta aaatttattt tggacaaaat tacaaattac tatcttacct
      241 aatccacaat tacttcctat ttttgtacca tgaataccac caaaaaagac attagggcat
      301 taaccaaaga acaacttaga gaattttttg tttctcaagg cgataaagcc tttcgtggta
      361 atcagattta cgaatggttg tggagtaaaa aagtctatca atttgatgat atgactaata
      421 tttctaagga aacccgtcag atgctggaag ataattttgt tatcaaccac atcaaagttg
      481 atcaaatgca acgcagtgaa gatggaaccg taaaaaatgc tgttcgatta tatgatggtt
      541 tggtggtaga aagcgtttta ataccaacag ctacccgaac aacagcctgc gtttccagtc
      601 aggttggttg cagtttagat tgtaattttt gtgcgacagc tcgtttaaaa cggatgcgaa
      661 acctgaatcc ggatgaaatt tatgaccaag tagttgccat cgataacgaa agccgtttgt
      721 actttgatcg accactttcc aatattgttt ttatgggaat gggtgagccg ttgatgaatt
      781 acaacaacgt actcaaagcg attgaaatga ttacttcgcc tgaaggattg ggaatgtcgc
      841 ctaaacgaat tacactttct acttccggaa ttcctaaaat gataaaaaaa atggccgatg
      901 aagaagttaa gttcaaatta gcggtttctc ttcattcagc catcgatgaa attagggccg
      961 aaattatgcc gttttcaaaa agttttcctt taaaggaatt acgtgaaagt ttggaatatt
     1021 ggtaccaaaa aaccaaaagc aaaattactt acgaatatgt ggtttggaaa ggaatcaatg
     1081 atgataaaaa atcgattgat gctctggtta aattttgtaa atatgtgcca tgtaaagtca
     1141 atttaattga atataaccca attgacgatg gaatgtttca acaagcttct gatgatgcat
     1201 tagaacatta cgtttctgct ttggataaaa ttggtgtgat tgccaaagta agaagaagtc
     1261 gagggaaaga tattgatgct gcttgtggtc agttggcgaa taaaaatgaa gcaatatcct
     1321 aattatggct attgtgattt tctaactttc tgactatctt tgaactttca tgaaaatcac
     1381 cgaacaaatt aaacaaccca tactcgctga aatggaactt tttgaaaaaa agttctatga
     1441 atcgatgtct tctaaagtgg cgttgctcaa ccgaataacc tattatattg tcaaccgaaa
     1501 aggaaaacaa atgcgtccga tgtttgtttt tctgacggca aaaatggtat ccgacggaat
     1561 cgtaaatgaa cgaacttttc gaggagcctc ggttatcgaa ttgattcata cggcaacgtt
     1621 ggttcatgat gatgttgtgg atgatagcaa tcgtcgtcgt ggatttttct ctatcaatgc
     1681 attgtggaaa aacaaaattg ctgttttagt gggtgatttt ttactatcta aaggtttgct
     1741 gctttccatt gacaacaacg attttgactt attgaaaata atctctgttg ctgttcgcga
     1801 aatgagtgaa ggagaattgc ttcaaattga aaaagcccgt cgtttagata ttacagaaga
     1861 catctattat gaaattatcc gtcaaaaaac agcaacatta attgccgcat gttgttcttt
     1921 gggagcttgt tccgttttgc cggaaaataa ggaagtagtt gaaagaatga gaaagtttgg
     1981 tgaactaata ggaatggctt ttcaaataaa agacgattta tttgactata ctgaggaagc
     2041 tattggtaaa ccaactggga ttgatatcaa ggaacaaaaa atgacacttc ctttaattta
     2101 tacgttgaat acttgttcct caaaagagaa aagttggctt atcaactcta ttaaaaacca
     2161 taatttggat aaaaaaagag tcaaagaagt cattgccttt gtgaaaagca gtaatggctt
     2221 gacttatgct gaacaaaaaa tgatccaatt tcagcaggaa gctcttttgc ttttagaagn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aaaaatgaaa gtaataaact
     2401 tacatcaaga cgaacagact accattcgaa tggctatcga acaaaatcga tttgctcagc
     2461 agcaaattta tgccaagttt tcgcctaaaa tgttaagtgt ttgccgacaa tatattaaag
     2521 atttgcagca agcggaagat gttatgttaa cctcatttat gaaggtattt acgaatctta
     2581 aaaaatttga gcataaagga agttttgaag gttggattag gcgaatcatg attaatgaat
     2641 gtgtttcatt tataagagtt caaaaacaag tcaaattcat cgaagaagaa actttttttg
     2701 aggaaagaca taatgatatt gaaagcaaat tttcggttga tgatattcag tttctcattg
     2761 atggtttacc ggatggttac cgtatgattt tcaatcttta tgcaatagaa gggtacaaac
     2821 atcaggaaat agcagtaatg ttaggaataa acgaagggac atcaaaatcg caattatctc
     2881 acgccagaaa actactgcaa gaacagatta ccaaattaaa aaattacgac aatggaaccg
     2941 aataagttag aaaataaaat gagagaaaag ttgaataaac gaacaattca accgtcagcc
     3001 aatgcatggg atcgtttaga tgcgatgctc tctgttacag aacaaaaacc gaaaagaaat
     3061 tttaaatggc tttccattgc agctgttttt gttggattca cattgattgg aatttttata
     3121 ttgaataaag aaaaatctat tgaaaatgta atccagtcta acccaattgt tttggaaaac
     3181 gaaacagaaa aagttgaaaa tctaaacgaa ataggaaaag aaaatactat ttcatccgaa
     3241 gaaaatcagg aagaagttgt cctaataaat caatcaaaga aaaaaatgga tgaacaacct
     3301 attgcaatca atcccaagaa agatttttta ttagataact attccaaaac agaagaagta
     3361 atcgttgaaa atcagccaaa agaagcgata tataaatacg tcaatgccaa atcacttctg
     3421 gctgaaatcg aaacaggaga aaaacttgaa acaccaacac tttcaaaaaa accaactgta
     3481 aaagtagatg ctaatgcttt actttcttcg gcagaaaaag aagttgagga aacatttaga
     3541 gacaaagtta ttcaatcgat taataaaaaa tacaattcgg taaagtcaac gttggctaat
     3601 cgcaataacg agtaaaaaca atcaaatcac gaacagttaa attttaaaat tatgcaaaaa
     3661 attatttttt atgtagcagt attgctatgc actttcctaa cccaaattta cggacaggaa
     3721 acctttgaac aacaagctaa agttattgct aatagtattt caagtataac aactgaggaa
     3781 aaagcagctt taaaagcgga agtagaagaa atcaacaaat tacttgaaag tggaaccatt
     3841 actgcaactc aagcagatga aaaaaaaata aaagcggcag aagaaaaagc caaaaacatt
     3901 gaacgcagag tggctaccga acaagcaaaa ctagatattt tagttaaaga tgttgtggat
     3961 ggtaaagtga attattcaaa agaaaaggag aaatggggaa caactatcat tttaggtaca
     4021 aattctaacg attcaattgg tgaaaatcat acagagatta gtttaggttc aatgaaagtt
     4081 tacaaaggcg aaacagagaa aatcagaaga aattcaaaaa gaacgacttc tcagtttgtt
     4141 tttgcctttg gattaaacaa tttaattaca gatggggatt ctaattctct tgaaaatagc
     4201 gattttaaaa cttggggctc taatttcttt gaatggggat ttacttttaa taccagaatt
     4261 ttgaaagata ataatttgct tcattttaag tacggattgt ctttaatgta caataatttg
     4321 cgaccaacag ataatcgcta ttttgtaaaa gatggcgatg tgacgcagct gcaaacgtat
     4381 gatagagatt taaatgaaaa taaattcaga aattctatgg tggtgttacc tgttcatctg
     4441 gagtttgatt ttacgccaaa aaaagtgggt gatgatggca gttttaaatt cagaacacac
     4501 aaaagcgtgc gattaggagt gggtggtttt ggaggtttaa actatcaatc tactcaaaaa
     4561 ttaagatatg aggaagacga tattcgaatc aaaaataaac aaaaaggaga ttttaatgtg
     4621 tcgacttttg tttatggatt gagtggttat cttggttatg gttcaacttc attatatgta
     4681 aaatacaatt tgaacagttt atttaaagat aatcctattg atcaaaacaa catttctttg
     4741 ggattgcgtt tcgattttaa ttaattactt gaattagttt ttttaaaccc tttcagagtt
     4801 tcttctttga aagggttttc tatttaaaaa ctttgtaact ttgaaatctt aattttttta
     4861 cttgattaca caaaatacca tcgatttagt ttttgaaacc gcccgtgttg aggaggtgat
     4921 tggtgatttt gtgcaattga aacgtgccgg aagtaatatg aaagggctga gtccgttttc
     4981 aaacgaaaaa tcgccgtctt ttatggtttc cccggtaaag cagatttgga aagattttag
     5041 caccggaaaa ggcggaaatt cagttacttt tttgatggaa cacgaacatt tttcgtttcc
     5101 ggaagccatt cgctacttag caaaaaagta caatatcgaa attgaagaaa cggtttcaag
     5161 cgacgaagat aagttggttg ccaatgaaaa agagagcatg tatttggttt ctgaatttgc
     5221 gagtaagtat tttcaacatg ttttgtggga agaagaagaa ggcaaggcaa ttggtttgtc
     5281 ttattttaaa gaacgtggtt tttcaaatga aactatcaaa aaattcaatt tagggtattc
     5341 accaaatacg tgggatgctt tgacaaaaga agctcttgga aaagcctata aattagaatt
     5401 tcttgaaaaa accggactca ccattgttgg tgaagacaag caatttgacc gattcaaagg
     5461 tcgtgttatg ttccccattc aaagtatgtc gggaagagta cttggttttg gtggtcgaat
     5521 tttgacgaat gataaaaaag ctgccaaata tctgaattct cccgaaagtg aaatttacca
     5581 taagagtaat gtactttacg gaatttttca tgccaaacag tccatcgcaa aactcgataa
     5641 ttgttttttg gtggaaggtt atactgatgt gattcaaatg catcaagccg gaatagaaaa
     5701 tgtggtttcc tcctccggaa cggcattaac accaaatcaa attcgattaa ttaatcggtt
     5761 aaccaaaaat attactgtcc tttttgatgg tgatgcagcc ggtttgaggg catctattcg
     5821 tggaatcgat ttgattttgg aagaagggat gaacgtaaaa gtttgtacat ttcctgacgg
     5881 ggatgacccg gatagttttg ccaaaaaaac accttatgaa gaattggtaa agtatttaga
     5941 agaaaattca aaagacttta ttcagttcaa agcgtctttg ttaatgaagg atgccaaaaa
     6001 tgacccaatc aaaaaagcgg atttgattcg ggatatggtg acaagcattt ctaaaattcc
     6061 ggaccgaatt aaaaaagaga tttatatcca ggaatgtgcc cgaattatgg atatttcaga
     6121 agaagtgctg ttcagtactt tagctcaaat ggctcaaaag gatttatcgg aagctaataa
     6181 aaaattcaaa caggaacaaa aagtatttga agttgtcaag aatgaaacaa cggcaacggt
     6241 ttcaaaagta gacatcattt cattacttga aaaaaccatt cttgaaattt tattattgta
     6301 tggtcatcat gaagtggagt ttgaaaatgt ctttattgat tatgacgaaa acgggaaaga
     6361 aattgaaatc aatgataaag taaatcaaaa aatttacgaa cggatttatt taagtttaca
     6421 ggaagatgaa attgaattgg caaatcctat tttcaatgcc atttatgttg atttagtttc
     6481 acaatatcat caaaatgaaa cgttagataa aaatcattac ctcaataact tgcttccgga
     6541 acatgctgaa atagttacgg atatcataat gaatgacgaa aaataccaat tgcatggttg
     6601 gcttgataaa aaacaggttt ttgtaaaaga aaaagatgat ttggaaatac tttcccgatt
     6661 ggtttctgaa acgattgtaa ctttcagaga ataccttatt aataaattga atcgggaatt
     6721 aatgctgcaa atgcaggaag aaattccaga actttcaccg gaagaaatca ttacaatgat
     6781 taatgattac aataagctta aagtaagcat
//
LOCUS       DAOTCW010000121         6770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_8188_length_6770_cov_46.907818, whole genome shotgun sequence.
ACCESSION   DAOTCW010000121 DAOTCW010000000
VERSION     DAOTCW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8188_length_6770_cov_46.907818"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /gene="gldM"
                     /locus_tag="P5335_09280"
     CDS             <1..1014
                     /gene="gldM"
                     /locus_tag="P5335_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HRZ75110.1"
                     /translation="KVKNKDGIEIEYLDYHFKGFPGIASLSKLSVWQGDIKKAETDVY
                     NTLLGKAAVAAASYSNYQAIVVLEKSAYFQGETVKGKVVLGRYDENTTPTKFAGPGKI
                     ENGQAVISLTAGSVGEQNINGQFSFLEDGKEIPLKFEGKYVVVPQPKEATISADKMNV
                     VYRGVVNPMSISFAGVSDVDVTASAPGLTKGSGTGKYNMSPGQGTEVMINVTAKLPGG
                     KSVSSKKPYRIKGIPAPVGAIGGEMYTVKGAKSRLEVSQVTAKLPDFDFDVKLNVTAF
                     TFKVPGQAAVIVSGDRVNAQCKAALARAGRGDVVTISDIKTQLVGSSIMLPRTAPVIY
                     EIQ"
     gene            1064..1978
                     /gene="gldN"
                     /locus_tag="P5335_09285"
     CDS             1064..1978
                     /gene="gldN"
                     /locus_tag="P5335_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HRZ75111.1"
                     /translation="MKMNKVIACLSVLLITQMSVAQSNLLNAKSPSEIGKKTAAQEES
                     DNDKPLQYGYVHDRDVLMGKMVWEIIDLDERINFPLYFPIDTANIGKDRRSLFDVLIK
                     EIKSGKITEVYYDDYFNTKKSLKDMASSFTYIDTTNAGREEINTYFDDYKSGAKVLDP
                     QYIDKRELSSIDVSGYKVKGYWYFDKRQGEMKYRLLGICPVSPEAREIGVDNPDVIEL
                     FWVYFPATRDVLHEWKAFNNENSAQPMTFDHLLNSRRFSGVIYKEENVYGDRQIMEYM
                     KDNALNQLLESERVKEKIRDFEQDMWNY"
     gene            2041..3093
                     /locus_tag="P5335_09290"
     CDS             2041..3093
                     /locus_tag="P5335_09290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRZ75112.1"
                     /translation="MKDYLIVGSGLAGIAFAEFALQNNKSITVFENYSQNSSLVAGGL
                     YNPVILKRFSEVWKAQEQISLAQDFYGVLEGKLKTHLDFKVPIYRKLFSIEEQNNWFV
                     ATDKPNLAPFLATELKHNKYNGIDSPFGFGEVLQSGYVDIALLLSKYQSYLTNQGLLW
                     NEAFIYGDLEVQNGYVVYKKERYKQVVFAEGFGMHANPFFSNLPLDGTKGELLVIRAP
                     ELKLDVIIKSSVFILPIGNDLYKVGATYNWEDKTPTPTAAGRNELLTNLKALVHCDFE
                     VVDHLAGVRPTVKDRRPIVGTHPKHPNLHVLNGLGTRGVMLAPAMAKALFENIENQIP
                     LEKEIDIKRFKKVSWD"
     gene            complement(3090..3479)
                     /locus_tag="P5335_09295"
     CDS             complement(3090..3479)
                     /locus_tag="P5335_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRZ75113.1"
                     /translation="MLKKGSKLNSILTGSCPKCQEESMYLEKNPYIVSKIFKMHDTCS
                     HCGTRYKMEPSFFYGAMYVSYGLSIAFGVAAFIISNVFIGLGLIHSFIAIVITLILTY
                     PIILRLSRNIWINMFVHFDKDWKNKQR"
     gene            3546..5459
                     /locus_tag="P5335_09300"
     CDS             3546..5459
                     /locus_tag="P5335_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRZ75114.1"
                     /translation="MLNIHNLSVSFGGTFLFEEVTFRLGSGDRVGLVGKNGAGKSTML
                     KILAGDFKPDSGSIATEKEVKMGFLRQDIDFELGRTVLEEAYQAFEDIKKTEVRIDQI
                     NHLLATRTDYESQSYSELIDELSDVTHHFEVLGGYNYVGDTEKILLGLGFKREEFNNQ
                     TDTFSGGWRMRIELAKLLLQSNDILLLDEPTNHLDIESIIWLEGFLRNFPGVVVIVSH
                     DKMFLDNVTNRTIEISLGKAYDFNKPYSQYLVLREEIREKQLATQKNQAKKIEETEKL
                     IEKFRAKASKASMAQSLIKKLDKVERIEVDEDDNSVMNISFPVSITPGRVVIEAEHVT
                     KSYGDKTILKDISLLVERGSKIAFVGQNGQGKSTFIKAIVNEFKYQGSIKLGHNVQLG
                     YFAQNQAEYLDGEITLLETMVNAATDSNRSKVRDMLGSFLFRGDDVEKKVKVLSGGER
                     NRLALCKLLLQPINVLLMDEPTNHLDIKSKNVLKAALNKFEGTLLLVSHDRDFLQGMA
                     NTVYEFKDQKIREYLGDINFFLEQRNAMNMREIEKKDVVANSNSNKENKSVSYEDQKK
                     NKSLQNRLSKIESQIKQLEIDIQNDDKALMSNYDKHIEDASFFMAYNKKKQELDKLLE
                     DWEVIQGEIDALN"
     gene            5468..5854
                     /locus_tag="P5335_09305"
     CDS             5468..5854
                     /locus_tag="P5335_09305"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HRZ75115.1"
                     /translation="MIRKNLHLIISVLIVISAALFYGLFPNVTLPVLFDFEVQSTDLK
                     NVFRAILGLYLAFSALWVLGILKSDFWKMATLSNFIFMIGLVSGRMLSMVLDGIPSPA
                     FSYGIIGELILAIFALYQYRKYKNSI"
     gene            complement(5847..>6770)
                     /locus_tag="P5335_09310"
     CDS             complement(5847..>6770)
                     /locus_tag="P5335_09310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRZ75116.1"
                     /translation="FGERDKCVKTFVRNLIFRNFCSHVLLLGNNMLSGQNAYQFSEQI
                     DSAKGIEVLYQAIDEIKKILKQKGLSVHLTTLKDFEEKEQLHFSAPQFQSFYRFTTQP
                     NMVFCSCDHWHTFDDYIASLSKKYRDQYKRCRKKAAGIHKQKMNLEEIIAQEETIYDL
                     YHHVAQNAPFNTFFLAKNHFRVMKEKLKDNFLFYGYFENGQLIGFNTLIKNGTQMDTY
                     FLGYDEKIQREKMLYLNMLYDMIAYSINKGFKEIIFARTALEIKSSVGAKPEEMFGFM
                     QHSNVLINKKMEQLFCYLEPKTDWQERNPFK"
BASE COUNT         2307 a          991 c         1286 g         2186 t
ORIGIN      
        1 aaagtaaaga ataaagatgg tattgaaatt gaatatttag attatcattt taaagggttt
       61 ccaggtattg cttcattatc aaaattgtca gtttggcaag gtgatataaa aaaggctgaa
      121 actgatgttt ataatacatt gttaggtaaa gcggctgttg ctgctgcctc gtacagtaat
      181 tatcaagcaa ttgttgtttt agagaaaagt gcatatttcc aaggtgaaac agttaaaggt
      241 aaagttgtac taggtcgtta tgatgaaaat acaacaccta ctaaatttgc cggtcctggt
      301 aaaattgaaa acggtcaagc cgttatttca ttaacagctg gttctgtagg agagcaaaat
      361 attaatggac agtttagttt cctagaagac ggaaaagaaa ttccattaaa atttgaagga
      421 aaatatgttg ttgttcctca acctaaagaa gctacaattt ctgctgataa aatgaatgtc
      481 gtttacagag gtgttgtgaa cccaatgtct atttcttttg cgggtgtttc ggatgttgat
      541 gttacagctt cagctcctgg tcttaccaaa ggatcaggta ccggtaaata taatatgagt
      601 ccgggtcaag gaactgaagt gatgataaat gttactgcta aattaccagg tggtaaatct
      661 gtaagttcaa aaaaaccata tcgtatcaaa ggtattcctg ctccggtagg tgcaattgga
      721 ggagaaatgt atacggttaa aggtgcaaaa tcaagattag aagtttctca agtaactgct
      781 aaattacctg atttcgattt tgatgtgaaa ttaaatgtta ccgcatttac atttaaagtt
      841 cccggtcaag cagctgttat tgtttccggt gatagagtaa atgctcaatg taaagcagct
      901 ttagcaagag caggtagagg agacgtagtt acgatttctg atatcaaaac acaattggtt
      961 ggttcttcta ttatgttgcc aagaactgct ccagttattt acgaaataca ataattaggt
     1021 aagattttcg ttattttctt aattaaccta caaattatac acaatgaaaa tgaataaagt
     1081 tatagcgtgc ctgtcggttt tgttgattac tcaaatgagt gtcgcacaat ccaatttatt
     1141 gaatgctaaa tcaccaagtg aaattggcaa aaaaacggct gctcaagaag agagtgataa
     1201 cgacaaaccg cttcaatacg gttacgtgca tgatagagac gtattaatgg gtaaaatggt
     1261 ttgggaaatc atcgatttag atgaacgtat taatttccca ttatatttcc ctattgatac
     1321 tgctaatatc ggaaaagaca gacgttcttt atttgatgtg ttgattaaag aaattaaaag
     1381 tggaaaaatt acggaagtat attatgatga ttactttaat accaaaaaat cattaaaaga
     1441 tatggcttct tcctttactt acattgatac tacgaatgcc ggtagagaag aaatcaatac
     1501 ttattttgac gattataaat caggtgcaaa agtattagat cctcaatata tcgacaaaag
     1561 agaattaagt tctattgatg tttcaggata taaagtaaaa ggatattggt attttgacaa
     1621 acgtcaaggt gaaatgaaat accgtttatt aggtatttgt ccggtatctc ctgaggctcg
     1681 tgaaataggt gttgacaatc cggatgtaat cgaattattc tgggtttatt tcccggctac
     1741 acgtgatgta ttacatgaat ggaaagcatt caataatgaa aattctgctc agccaatgac
     1801 ttttgatcat ttattaaatt cacgccgttt tagtggtgta atttataaag aagagaatgt
     1861 atacggagac agacaaatta tggaatacat gaaagataat gccttaaatc aacttttaga
     1921 atctgaaagg gtgaaagaaa aaatcagaga ctttgagcaa gatatgtgga attattaatt
     1981 cataaaatta aatacaataa aaactcttac ttcacggtaa gagtttttta tttttgtaac
     2041 atgaaagatt atttaatagt tggctccggt ttagccggta ttgcttttgc tgagtttgct
     2101 ctccaaaata ataaatcgat tactgttttt gaaaattatt ctcaaaattc ctctcttgtt
     2161 gcgggtggct tatataatcc tgttattctt aaacggttta gtgaagtatg gaaagcacaa
     2221 gagcaaatat cattagccca agatttttat ggtgttttgg aaggtaaatt aaaaacgcac
     2281 cttgatttta aagtgccaat ttatagaaag ttgttttcca tcgaagaaca aaataattgg
     2341 tttgttgcta ccgataaacc aaatcttgca ccttttttag ctacggaatt aaagcacaat
     2401 aaatacaacg gaattgattc tccatttggt tttggcgaag ttttacaatc aggatatgtt
     2461 gatattgctt tattacttag caaataccaa agttatctaa ctaatcaggg attattatgg
     2521 aatgaagcat ttatttacgg cgatttagaa gtgcaaaatg gatatgttgt ttataaaaag
     2581 gaaagataca agcaggttgt ctttgcggaa ggctttggga tgcatgctaa tccatttttt
     2641 tctaatttac cactcgatgg aactaaaggg gaattattag tcattagagc tcctgaattg
     2701 aaactggatg taattattaa atcatccgta tttattttgc ccattggaaa cgatttatat
     2761 aaagttgggg caacttacaa ttgggaagat aaaacaccaa cacccacagc agcaggaaga
     2821 aatgaattat taactaatct gaaggcactg gttcattgcg attttgaagt tgttgaccat
     2881 ttagccggtg ttagaccaac ggtgaaagac agaagaccaa ttgtaggaac tcatccaaaa
     2941 catccaaatc tccatgtatt aaatggatta ggaacccgag gggtaatgct ggcaccagca
     3001 atggctaaag cgttatttga aaatattgaa aatcaaattc ctttggaaaa agaaatagac
     3061 atcaaaaggt ttaaaaaagt tagttgggat taacgttgtt tgtttttcca atctttatca
     3121 aaatggacaa acatgttgat ccaaatattt ctggacaatc gcaagataat cggataagtt
     3181 agaattaaag taattactat cgcaataaaa gaatgaatta gtcctaatcc gataaaaaca
     3241 tttgaaatta taaaggctgc aacaccaaag gcaatactca gtccataact cacatacatc
     3301 gctccataaa aaaaagaagg ttccatttta taacgagtgc cacaatgact acaagtatca
     3361 tgcattttaa atattttact cacgatatat ggattttttt ctaaatacat actttcttct
     3421 tggcatttag gacaacttcc tgttaaaatg ctattcaatt tggatccttt ttttaacatt
     3481 tgcaaaaatt ttatacaaag gtatcttttt cacaagttaa ccaatgtaac atttgtaaca
     3541 aaattatgct aaacatacac aatttatcgg tttcctttgg aggaacattt ttatttgaag
     3601 aggtaacctt tcgtttgggt tcaggggaca gagttggatt agtgggaaaa aatggtgccg
     3661 gtaaatcaac catgcttaaa attttagcag gtgattttaa acctgattct ggaagtattg
     3721 ccacagagaa agaagtaaag atgggttttc tacgtcagga tatcgatttt gaactgggaa
     3781 gaactgtatt agaagaagct taccaagctt ttgaagatat taagaaaacg gaagtcagaa
     3841 tagatcaaat caatcatcta ttggccaccc gtacagatta tgaaagccaa tcttactctg
     3901 aattaattga tgaattgagt gatgtaacgc atcattttga agtattaggt ggttacaatt
     3961 atgtgggtga tacggagaaa attctgttag ggttaggttt caaaagagag gaattcaata
     4021 atcaaacgga tactttttca ggaggttggc ggatgcggat tgaattagca aaactgcttt
     4081 tacaatccaa cgatattttg cttctcgatg agccaaccaa tcacttagat attgaaagta
     4141 taatttggct agaaggtttt cttagaaatt ttcccggagt agtggtcatc gtttcgcacg
     4201 ataaaatgtt tttagataat gtgacgaatc gaaccatcga aatctcttta ggaaaagcct
     4261 atgatttcaa taaaccgtat tcacaatatt tagttttgcg agaagaaatt cgcgaaaagc
     4321 aattggctac tcaaaaaaat caggctaaaa aaattgaaga gactgaaaag ttaatagaaa
     4381 agtttagagc aaaggcttca aaagcgtcca tggctcaatc gttaatcaag aaattagata
     4441 aagtggaacg aattgaagtc gatgaagatg ataattctgt gatgaatatt tcctttcccg
     4501 tttctataac acccggtaga gtggtgattg aggcagaaca tgtgacgaaa tcttatggtg
     4561 ataaaaccat tctaaaagac atttcattat tggttgaacg tggtagcaaa attgccttcg
     4621 tgggtcaaaa tggtcaagga aaatcaacgt ttatcaaagc catcgttaac gaattcaagt
     4681 accaaggttc aattaaatta ggacataatg ttcagttggg ttattttgcc caaaatcagg
     4741 cggaatattt agacggcgaa attacgttgt tggaaaccat ggtgaatgct gcaacagaca
     4801 gtaataggtc taaagttcgc gatatgttag gttccttttt gtttcgaggg gatgatgtgg
     4861 aaaaaaaggt aaaggtgcta tccggtggtg aacgaaaccg tttggcatta tgcaaattat
     4921 tgttacaacc cattaatgtt ttattgatgg atgaaccgac caatcactta gatattaaat
     4981 caaaaaacgt tttaaaagcg gccttaaata aatttgaagg aaccttattg ttggtttcac
     5041 acgataggga ttttttacaa ggaatggcaa atacggtata cgaattcaaa gaccaaaaaa
     5101 tcagagaata tttaggagat atcaactttt tcttagagca acgtaacgcc atgaacatgc
     5161 gtgaaattga gaagaaagat gtagttgcga attcaaattc caataaggaa aataaatcgg
     5221 tttcgtatga agatcaaaag aagaacaaat cgcttcaaaa tcgcttaagt aaaattgaaa
     5281 gtcaaatcaa gcaattggaa atcgacattc aaaatgatga taaagcattg atgtcaaatt
     5341 atgataaaca tatagaagat gctagttttt ttatggccta taataaaaag aaacaggagt
     5401 tagataaatt attggaggat tgggaagtta ttcagggaga gatagatgct ttaaattagg
     5461 gggcaatatg attcgtaaaa atttacattt gattatttca gtgttgattg ttatctccgc
     5521 agcacttttt tacggattat tcccaaatgt aactttaccc gttttatttg attttgaagt
     5581 tcaatctact gatttaaaga atgtgtttcg agctattctc ggattatatt tggctttttc
     5641 agcactttgg gttcttggaa tactaaagtc tgacttttgg aaaatggcca cattgtctaa
     5701 tttcatattt atgattggat tagtatctgg tagaatgttg agtatggttt tggatggcat
     5761 tccttctccg gctttttctt atggtattat tggcgaattg attttagcga tttttgcctt
     5821 atatcaatac cgaaaatata aaaattctat ttaaacggat ttcgctcttg ccaatctgtt
     5881 ttaggttcca aataacagaa taactgctcc atttttttat tgattagcac atttgaatgc
     5941 tgcataaatc caaacatttc ttccggcttt gcaccaacag aacttttaat ttccaaagct
     6001 gttcgggcaa aaataatttc cttaaaccct ttattgatgg aataggctat catatcatac
     6061 agcatattta aatacaacat tttttcacgt tgaatttttt catcataccc taaaaaataa
     6121 gtatccattt gggtcccgtt tttaattaag gtattaaatc caattaattg gccattttca
     6181 aaataaccat aaaataaaaa attgtctttc agtttttctt tcatcacccg aaagtgattt
     6241 ttggccagga aaaaagtgtt aaacggagca ttttgagcga catgatgata caagtcataa
     6301 atggtttctt cctgagctat aatttcctct aaattcattt tttgtttatg aattcctgct
     6361 gcttttttac gacagcgttt gtattggtct ctgtattttt ttgataagga ggcaatataa
     6421 tcatcaaagg tatgccagtg gtcacaggag caaaaaacca tattaggttg agttgtaaaa
     6481 cgataaaaag attgaaattg cggtgcagaa aaatgaagtt gctctttttc ttcaaaatct
     6541 tttagggttg ttaaatgcac actgagccct ttctgtttta gtattttttt gatttcatct
     6601 attgcttgat aaagtacttc tattccttta gcggaatcaa tttgttcaga aaactgatag
     6661 gcattttgac cggacaacat gttgttccca aggagtaaaa catgcgaaca gaagtttctg
     6721 aaaataagat tccgaacaaa tgttttgaca cacttgtccc gttcaccaaa
//
LOCUS       DAOTCW010000122         6602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_8502_length_6602_cov_46.564381, whole genome shotgun sequence.
ACCESSION   DAOTCW010000122 DAOTCW010000000
VERSION     DAOTCW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8502_length_6602_cov_46.564381"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="P5335_09315"
     CDS             complement(<1..758)
                     /locus_tag="P5335_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRZ75117.1"
                     /translation="MDISKIHSFFLQCSSVSIDTRKILTNSFFVALKGTNFDANTFAK
                     QALENGAAYVVIDNRDYYIDERTILVEDSLNCLQELAKYHRNYLKTPIIALTGSNGKT
                     TTKELFQAVLSQKYTTKATVGNLNNHIGVPLTLLSFTKETEIGIVEMGANHQQEIAFL
                     CDLAKPDFGYITNFGKAHLEGFGGVEGVIKGKSEMYDFLQKFNKKVFVNFDDAIQFKK
                     TASMDRIVFSLVNHKADVFIEKVEANPFVKVVYDG"
     gene            complement(825..2537)
                     /gene="gldJ"
                     /locus_tag="P5335_09320"
     CDS             complement(825..2537)
                     /gene="gldJ"
                     /locus_tag="P5335_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldJ"
                     /protein_id="HRZ75118.1"
                     /translation="MKVNKNVTLKLLLMLVVMFGFTSCSKKSNSKNSSTATGWKINDK
                     KGGFQYASNFKKQDAAPGLIAIEGGTFTMGRVQDDVMHDWNNSPNQQHVQSFFMDESE
                     VTNLMYMEYLDWLKRIFDPNQDNYKNIYVGALPDTLVWRDRLGYNETMTNNYLRHPAY
                     KDYPVVGVNWIQAVEFCKWRTDRVNEDILEKNKYLKKDAKLMDATAEKSFSTETYINS
                     PTQTFGGDEEIVLKGKGGKSKPSTNREGETVEQKNVYAQRTSGLIQPEYRLPTEAEWE
                     YAAVANVGNREYNLYQGKKKYPWSGSYTRSGKRAVKGDQLANFKQGKGDYGGIAGWSD
                     DGADITQVVKSYPPNDFGLYDMAGNVNEWVADIYRPIVDDEANDFNYYRGNVYMKNKI
                     GEDGKVEIVTSETIKYDTLANGKIIARNYPGQIAQVPVDEKETYLRQNFDKSDNRNYR
                     DGDKQSTRFFDFGAEDDGGKVAESKKMYDSPQHQVSVDTASSEMIRKYDKSSKRTTLI
                     DDNVRVYKGGSWRDRAYWLDPAQRRYFPQEISTDDIGFRCAMSKVGPKSNKKSAKGKP
                     KASR"
     gene            2743..6582
                     /gene="porU"
                     /locus_tag="P5335_09325"
     CDS             2743..6582
                     /gene="porU"
                     /locus_tag="P5335_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HRZ75119.1"
                     /translation="MKKILLLYIVFNTLFFYAQNSVKLDVSWQENKKLIFDTYNLSIP
                     QFQFANFYFNDVERSIYFNYKLTVTTPIEEKSLQISSVIYESISQNELGDLTIKNIPN
                     SINASIKNSNARGLTQILFSFSPIIKTNEGFKKVKSLTFSYTNSTSVNLKNETTFDFN
                     SIQNSVLASGDWYRFYIEKSGVYKISKNFLNQLGMNTNVNPQKIKIYGNGGRMIPLLN
                     SVEYPSDLAENAIQFIGEEDGVFNNEDYILFYAEGVDQWSTESLTNLNLYDTKSYYYV
                     TTQGDNGKRIPNIVQPEGTPTNVFTTFDDYQFYEKDLNNIGKIGRNWYGEQFVSQTPL
                     TFDFTFPNVDTTSPISITVNSAAVSFSNSNLKIKAGGQEQTATFSAIPSNSGTLFSTA
                     NNNLNITASENITVELTYNNSGVPSAKGYLNFITLKAKRNLKGFGKQFSFQNLASGSQ
                     LGIGEYQLTNASAISQVWDITSIYDVSRIENTGLANFTFKANLGQVRKYIAIDPADYY
                     VPLKESQSKVVNQNIKGTIFNNNQGQFEDIDYIIITPLNLASEAERLAQFHRVYSQLN
                     VKVITLDKIYQEFSSGKQDIGAIRNLIKYVYYNASTAEKRIKYLNLFGDASYDYKNRI
                     PNNTNIVPIFHSLVSNSLLSSTMSDDFYTMMDPNEGLMTSGADGLDLAVGRMLVSNLQ
                     DAKELVDKVLEYHDIKSYGRWRNLFTLVSDDVDESWESVIQQQLDNLGNTISIENPYV
                     NVMKYHSDSFVQEASAGGQRYPKVKQELKNAIEQGSLVFNYFGHGGEDQLASERLFEK
                     TDAQNLNNRYRYPLFVTVTCEFTRFDNPYRPTAGEYTYWNPKGGAISLVTTTRQIGVQ
                     TGQDINNFFSAYLYGFNSTEEISIAEALRRAKNDYNSSTLMVFYIGDPAIILPIPKPK
                     IVLTKVNDTPIESSTFIFNALSRVKLTGEVRNLNGDALLNDYNGDIAIQVFDKNINRN
                     TLGNDGTTNGSGLIIMNFQTLGETIFRGNASVSNGLFEFEFIVPRDISVPVGNGRISF
                     YAKTQQPTLQNQSGYDTSIKIGGINANAPEDNVGPTCRLYMNDETFISGGITNENPIF
                     LAFLEDENGINTASGIGHDIVAILDGDENKPYILNDYYETELNNYKKGRVRFPFFNLA
                     TGLHTITFRAWDVYNNPISAEIQFIVVGEDSISLTNVLNYPNPFVNYTQFWFSHNHPF
                     EPLDVQVQIFTITGKVVKTINQVVNTDGFLSREITWDGKDDFGDRIGKGVYIYKLTVK
                     SSITNKKSEKIEKLVIL"
BASE COUNT         2278 a         1150 c          978 g         2196 t
ORIGIN      
        1 ccatcataaa caactttaac aaaaggattt gcttcaactt tttcaataaa aacatcagct
       61 ttatgattaa ctaagctaaa aactattctg tccattgacg ctgttttttt aaattgaata
      121 gcatcatcaa aatttacaaa aactttttta ttaaactttt gaagaaaatc atacatttca
      181 cttttgcctt taatcactcc ttcaacacct ccaaaacctt ctaaatgagc ctttccaaaa
      241 tttgtaatat acccaaaatc aggcttggct aaatcacaca aaaaagcaat ttcctgttga
      301 tgattagccc ccatttcaac aattccaatt tcagtttctt tagtaaatga caataaggtc
      361 aaaggaactc caatatgatt atttagatta ccaacagtag ctttagtagt atatttttga
      421 gataaaacag cttgaaataa ttccttagtt gttgttttac cattactacc ggtcaatgca
      481 ataattggtg tttttaaata attcctatgg tatttagcta attcttgaag acaattcaag
      541 gagtcttcta ccaaaatagt tcgctcatca atataataat ctctgttatc aattacgaca
      601 taagcagctc cattttctaa agcctgtttg gcaaaagtat ttgcatcaaa atttgttcct
      661 tttaaggcaa caaaaaaaga attagttaat atttttcgag tatcaataga aacagaagaa
      721 cattgtaaaa aaaaagaatg aatttttgaa atatccataa actaaaaaaa attaaaaata
      781 aaaagcccta aaaaagggct tcttaaataa ataaacaata aatgttatct tgatgctttt
      841 ggtttccctt ttgccgattt tttattagat tttggcccaa ctttagacat cgcacatcta
      901 aaaccaatat catctgttga aatttcttgt ggaaagtatc ttctttgagc aggatctaac
      961 caataagctc tatctctcca agaacctcct ttataaactc ttacattgtc atcaataagg
     1021 gttgttctct tcgatgattt gtcatatttt ctgatcattt cagatgatgc agtatcaaca
     1081 ctaacttgat gttgtggaga atcatacatt ttttttgatt cagctacttt tcctccatca
     1141 tcttctgcac caaaatcaaa gaaacgagtt gattgtttat ctccatcgcg ataatttcta
     1201 ttatcacttt tatcaaaatt ttgtctcaaa taggtctctt tttcatcaac aggtacttga
     1261 gcaatttgac caggataatt tctggcaatt attttaccat tcgctaacgt gtcatatttt
     1321 atggtctcgc tggtaacaat ttctacttta ccatcttccc caattttgtt tttcatataa
     1381 acgttacctc tgtagtagtt gaagtcattc gcttcatcat ccacgatagg acggtaaata
     1441 tctgctaccc actcatttac atttccagcc atatcataca aaccaaaatc atttggcgga
     1501 tatgacttaa ctacttgcgt aatatcagca ccatcatcag accaaccagc aattccgccg
     1561 tagtcacctt ttccttgttt aaagtttgct aactgatcac cttttactgc tcttttccct
     1621 gaacgggtat aactaccaga ccatgggtat ttcttcttac cttggtaaag attatactct
     1681 ctatttccaa catttgcaac agcggcatat tcccattctg cttcagttgg aagtctatac
     1741 tcaggctgaa ttaatcctga agttctctgt gcataaacat ttttttgctc aactgtttct
     1801 ccttcacgat tggttgaagg tttagactta ccacctttac ctttaagaac aatttcttca
     1861 tcaccaccaa aagtttgagt aggagaattg atatatgttt cggtgctaaa tgatttttct
     1921 gccgtagcat ccattaattt agcatccttt ttaaggtatt tatttttttc taaaatgtct
     1981 tcattaacac ggtctgttct ccatttacaa aactctactg cttgaatcca atttacacct
     2041 actacaggat aatccttata agcaggatgt ctcaagtaat tatttgtcat ggtttcatta
     2101 tatccaagac gatctctcca aacaagagtg tcaggcaaag ctccaacata aatgttcttg
     2161 tagttgtctt gatttggatc gaaaattctt tttaaccagt ctagatactc catgtacatt
     2221 aggttagtta cttcactttc gtccatgaaa aatgactgaa catgttgctg atttggcgaa
     2281 ttattccaat catgcattac atcatcttga actcttccca tagtaaaagt tccaccttca
     2341 atagcaataa gaccaggggc agcatcttgt tttttgaagt tagaagcgta ctgaaaacca
     2401 ccttttttgt cgttgatttt ccaaccggtt gcagttgaag aatttttaga gtttgacttt
     2461 ttactacaac tagtaaaacc aaacataact actaacatta gtagcagttt taaagtcaca
     2521 tttttgttta ctttcatact tttaagtagg taaaatttag tgccgcaata taggaattaa
     2581 cctttatatt acaacagttt tttaaaaata ttgtcaaaaa tacaaattta gcgtttttta
     2641 acaaacttat aacgcaaaaa atatttattt agtatgtgta aaaaaacatt gaaaaaatta
     2701 tacattgtaa taaatttgca gaccttacgt ttattcctag atatgaaaaa gattttactc
     2761 ctttatatag tatttaatac cttatttttt tatgctcaga actctgtaaa gctagatgtt
     2821 tcttggcaag agaataaaaa attgattttt gacacctata acctatctat tcctcaattt
     2881 caatttgcta atttttattt taacgatgtt gaaaggtcaa tttattttaa ttacaaacta
     2941 actgttacaa ctccaatcga ggaaaaatcg ttacagattt cgtctgttat ttatgaatct
     3001 atttcacaaa atgaactagg cgatttgaca ataaaaaaca ttccaaacag cataaatgcc
     3061 tctataaaga atagtaacgc aagaggttta acacaaattt tattctcttt ttcgcctata
     3121 ataaaaacaa atgaaggttt taaaaaagta aaatcattaa ctttcagtta taccaattct
     3181 acttctgtaa atttaaaaaa tgaaacaact tttgatttta attcaattca aaattcagtt
     3241 ttagcttctg gagattggta tcgattttat attgaaaagt caggcgttta taaaatttcc
     3301 aagaatttct taaaccaatt ggggatgaat acgaatgtta atcctcaaaa aattaaaatt
     3361 tatggcaatg gaggccgaat gattcctttg ttaaatagtg ttgaataccc ctctgattta
     3421 gcagaaaatg caatccaatt tattggagaa gaagacggcg tttttaataa tgaggattat
     3481 attttatttt atgcagaagg tgttgaccaa tggagcactg aaagcttgac taatttaaat
     3541 ctttacgaca caaaatcata ttattatgta actacacaag gtgataatgg taaaaggata
     3601 cctaatatag ttcagcctga aggcactcct actaatgttt ttacaacttt tgatgattat
     3661 caattttatg aaaaagacct taacaacatt ggcaaaattg gaagaaattg gtatggtgaa
     3721 caatttgttt cccaaacacc actaactttt gattttactt ttcctaatgt tgacacaacc
     3781 agcccaatat caattacagt aaactctgca gcggtttcct tttccaattc caatttaaaa
     3841 ataaaagcag gaggacaaga acaaacagct acttttagtg caatcccttc aaatagcggt
     3901 accctttttt ctactgcaaa caataactta aacatcactg caagtgaaaa cattacagta
     3961 gaactcactt acaataatag tggtgtgcct agtgctaaag gatatttaaa ctttataacc
     4021 ttaaaagcaa agagaaattt aaagggattt ggtaaacaat ttagttttca aaatttggca
     4081 tccggttcac aactaggaat tggagaatat caacttacga atgcttctgc catttctcaa
     4141 gtctgggata taacttcaat ttatgatgta agtagaatag aaaatacggg cttagctaat
     4201 tttactttca aagcaaactt aggacaagtc agaaaatata ttgcgattga tccagctgat
     4261 tattatgtcc ctctaaaaga atcgcaatca aaagttgtca atcaaaatat taaaggcact
     4321 attttcaaca acaatcaagg tcagtttgaa gatatcgatt atatcattat tactccctta
     4381 aatttagctt cagaagcaga aaggttggca caatttcaca gagtatattc tcaattaaat
     4441 gtaaaagtca tcacacttga taaaatttat caggaatttt catcaggcaa acaagatata
     4501 ggtgctatta gaaacctaat caaatacgtt tattataatg cctcaaccgc tgaaaaaaga
     4561 ataaaatact taaatctttt tggtgatgct tcctatgatt acaaaaatcg aattccaaac
     4621 aacacaaaca ttgtcccgat ttttcacagc ctagtgagta attcattgtt aagctcaaca
     4681 atgtccgatg atttttatac tatgatggat ccgaatgaag gattaatgac ttcaggagct
     4741 gatggtttag atcttgcagt aggaagaatg ttggtttcta atttgcaaga cgctaaagaa
     4801 ttagtagata aagttcttga ataccacgat attaaatctt atggcagatg gcgtaactta
     4861 ttcacattag tctctgacga tgtggatgaa agttgggaaa gcgttattca acaacaattg
     4921 gacaacttag gaaatacaat cagtattgaa aatccatacg taaatgtaat gaagtaccat
     4981 tctgattctt ttgttcaaga agcttcagct ggaggacaac gttatcctaa agttaaacaa
     5041 gaattgaaaa acgcaataga acaaggttca ttggtattta attattttgg tcatggtgga
     5101 gaagatcaat tggcttctga acgacttttc gaaaaaacag atgcacaaaa cttaaataat
     5161 cgataccgat acccattatt tgtgaccgta acctgtgaat ttaccagatt cgacaatcca
     5221 tatagaccaa cagcaggaga atacacctat tggaatccga aaggaggtgc catttcattg
     5281 gttacaacaa cacggcaaat tggcgttcaa acaggacagg atataaataa ttttttctcc
     5341 gcctatttgt acggatttaa ttctaccgaa gaaatttcta ttgcagaagc attaagaaga
     5401 gcaaaaaatg actacaatag cagcacatta atggtgtttt acattggtga tccggccatc
     5461 attttaccca ttcctaaacc aaaaattgtt ttaacaaaag taaatgacac ccctattgaa
     5521 tcttctacat tcatattcaa tgcattaagc agagttaagt taaccggcga agttagaaat
     5581 ttaaatggtg atgctcttct aaatgactat aatggcgata ttgctattca ggtttttgat
     5641 aaaaacatta acagaaacac cttaggaaat gacggaacaa caaatggttc gggattaatt
     5701 atcatgaatt ttcaaactct aggagaaact atctttagag gaaatgcctc cgtttcaaat
     5761 ggtttatttg aatttgaatt cattgtgccc cgtgatatta gtgttcctgt tggaaatggt
     5821 agaatcagtt tctatgctaa aacgcaacaa ccaacgctgc aaaatcaatc cggttatgat
     5881 acttctatta aaattggagg tataaatgca aatgccccag aggataacgt tgggccaact
     5941 tgtcgtttat atatgaatga cgaaactttt atttccgggg gaatcactaa cgaaaaccct
     6001 atatttttag cctttttgga agatgaaaac ggaatcaata ctgctagtgg aatcggacat
     6061 gatattgtag ctattttaga tggcgacgaa aacaaaccct atattttaaa tgactactac
     6121 gaaacagaat taaataatta taaaaaagga agagttaggt ttccgttttt taatttggcg
     6181 acaggacttc atacaataac ctttagagca tgggacgttt ataataatcc tatttcagca
     6241 gaaattcaat ttattgttgt gggtgaagat tccatcagtc ttacaaatgt tttaaactac
     6301 ccaaaccctt ttgttaatta cacacaattc tggttttcac ataatcatcc ttttgaacct
     6361 ttagatgttc aagtccaaat tttcacaatt accggaaaag ttgtaaaaac tattaatcaa
     6421 gtagtaaata ccgatggttt tctctcaaga gaaattacgt gggatggaaa agatgatttt
     6481 ggtgaccgaa ttggcaaagg agtttacatt tataaactaa cagtcaaatc cagcatcaca
     6541 aataaaaagt ccgaaaaaat cgaaaaactt gtaatccttt aagaaaacac tatatttgtt
     6601 aa
//
LOCUS       DAOTCW010000123         6574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_8557_length_6574_cov_36.961651, whole genome shotgun sequence.
ACCESSION   DAOTCW010000123 DAOTCW010000000
VERSION     DAOTCW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6574
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8557_length_6574_cov_36.961651"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..758)
                     /locus_tag="P5335_09330"
     CDS             complement(108..758)
                     /locus_tag="P5335_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRZ75120.1"
                     /translation="MNIKLAIIDDNSFLIKAIQEKLSFFDDIQVKFSALNGAELLTKL
                     DVNHNIDVLLMDIDMPVLNGIEATAIVKQKYPHIKVIMLTVFDNDENIFNAIKAGADG
                     YLLKEVNPTDLHQGIIETLNGGAAMNPSIALKTLKLLRNPVQFESKIEEDEIKLSARE
                     IEVLEQLSKGLNYNQIADNLILSPSTVRKHIENIYNKLQVHNKLEAIQKAKSNKLI"
     gene            complement(769..1038)
                     /locus_tag="P5335_09335"
     CDS             complement(769..1038)
                     /locus_tag="P5335_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75121.1"
                     /translation="MNQFILGLLFLIYTLLIVYFKNKTNAQVKEHQKIMLHLLYQINK
                     EQNIKNERNDLTHENAIIEENTQNMLTAIQLKLEFLHLISTTNLS"
     gene            complement(1111..3024)
                     /locus_tag="P5335_09340"
     CDS             complement(1111..3024)
                     /locus_tag="P5335_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRZ75122.1"
                     /translation="MKKIIEKGFGLKYVFLILFFVQLANGQSFETYSAKQLNEKAMDI
                     HRTEPEKAMQLVKSAEKKALQLENKKELSVTYSNYAVLFRLKGEFKEAIKYAEKALKL
                     SNDSLLIASCNNTIGACKRSLGFYEEGLKNYLIALKIYESKKDIDQQGTVINNIGMIY
                     SQMDLLEKAKVYHRKAIAIFQKSKYQKGLSESYNNLAIALANQDSLTKALRYFNYSLQ
                     IEEKLKDKKGIAESYNNVAGIYYYLQQIDSAKYYFEKSVELEREIKNFPGVAASYNNL
                     ALVMLEFKRNASAKNYIDSAFYYAKKSKVAEDYVNALENYALYYEALSDYKTANDYNK
                     KFFTAKDSVFKNSNLENLHELEVKYKSAQKDAEISKNKAEIAENELQIKQKNTIIYGS
                     VGFAILLGLVGYLFYNQQKIKNIQLLKEVELKEALVKIETQNKLQEQRLQISRDLHDN
                     IGSQLTFIISSIDNLKYFDIAKEKLVSKFDNISGFTRNTITELRDTIWAMNKSSISVE
                     DLQIRITNFIDNAQLAVHGITFEFSIDPNVNSAHEFTSVEGMNVYRIIQEAVNNAVKY
                     AQAKQIIVHLKEENSVLFFNITDDGKGFSVAEVHLGNGLNNMQKRASEIGAEIIIDSK
                     ENQGTTVLIKYPI"
     gene            complement(3029..4900)
                     /locus_tag="P5335_09345"
     CDS             complement(3029..4900)
                     /locus_tag="P5335_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRZ75123.1"
                     /translation="MYQVVRNQKNDSIKIDALYKLSNIFLSSNLTEANKLNRQSFTLS
                     KRKYLDFGLAKSFNIKGIIFDINGKSDSALYCYQKAIYHSKKAKLFATQASAFNNIGL
                     LEWNKANYLNALKYYNLGLQIFEKINDKKGQANTQSNIGLIYQSLNDKINAEKYFFQS
                     LKLREEIHDYYGISVSYTNLGRFHEDANENEKAIFYYEKAISNKRKINDLRGIAITLN
                     NLSSLQYNERKIAEAYSSCLEAIQIGEQLEAPSILAYAYLGLTDIYRAKKDIVKAKEA
                     NELAKKYCIETEDQAGLKNYYYGEIQLALLQNDFKAAYLNQVSKDSLQNVIIGLEVQK
                     AVSEIETKYQVEKKEKDLARAKVKVAERELEIKQKNTQLYLSIGASVTFLLIGFLFYN
                     QQKLKNTQLIKENELKEALVKIETQNKLQEQRLQISRDLHDNIGSQLTFIISSIDNLK
                     YFDIAKDKLVSKFDAISGFTQNTITELRDTIWAMNKNEITLEDLQIRIINFIEKAKVA
                     THGIAFEFSIDKKCPSNLKFSSVEGMNIYRIIQETLNNSIKHSGCTSIKIQIYIANNT
                     LIIEISDNGKGFLLNEIIEGNGLSNCRKRAQELGAEIDFITLEKGSLVKLSLNHFNS"
     gene            complement(5411..5899)
                     /locus_tag="P5335_09350"
     CDS             complement(5411..5899)
                     /locus_tag="P5335_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75124.1"
                     /translation="MKKNIFKIGLVMITIGFFSCSNDDNDGGSTAQEELSFKVDGTSI
                     VADEFEAVLYTNAVAGGRYIDVYGSKNGNDFAEFHFPAKTGSFPAQQSFDMASCWLTY
                     ENGVNYPDNYFHSTSGTLTVTVCDTVANKIEATFNFTGNNSSVDKVITDGKLKINSIT
                     RQ"
     gene            complement(5915..6442)
                     /locus_tag="P5335_09355"
     CDS             complement(5915..6442)
                     /locus_tag="P5335_09355"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HRZ75125.1"
                     /translation="MKTISKLSVLVLLTATIFTSCSSDDGGGSALPSGSYVKASIDGA
                     DFQSTEQLTVSAYSTGNLVIQGSLMDGTTISLNLGAIDHSLETGVYNVNATNNFDVNF
                     GSCNFMKLVNMSAVAYNSVNCENATGTIEITYIDETKVEGTFSFVGKEVKADETCNGG
                     TVSITNGSFRGIFSN"
BASE COUNT         2080 a         1080 c          875 g         2539 t
ORIGIN      
        1 acgtccaatg gcttgtaata tgggaactaa aacattttta acttgagttt cggtagattg
       61 atttctgcga gtagccattt gtgattattt tacacgaaga taaaaattta aatcaactta
      121 tttgatttgg ctttttgaat ggcttctaat ttattgtgca cttgcaattt gttataaata
      181 ttttcgatat gtttccgaac ggttgaaggg gaaagtatca aattgtctgc gatttggtta
      241 tagtttagtc ctttgcttaa ttgttccaaa acctcaattt ctctggctga aagtttgatt
      301 tcatcttctt caattttact ttcaaattgc accggatttc gtagcaattt aagtgttttt
      361 aatgcaatag acggattcat ggcagctccg ccatttaatg tttcgatgat tccctgatgc
      421 aaatcggttg ggttaacttc tttcaacaaa tagccatctg caccagcttt aattgcatta
      481 aaaatatttt catcattatc aaaaacagtg agcattataa ctttaatgtg aggatatttt
      541 tgtttgacta tggcagtggc ttcaatgcca tttaaaacag gcatatcaat atccatcaat
      601 aagacatcaa tgttgtgatt aacatccagt tttgttaata attctgctcc atttaaggca
      661 gaaaacttca cttgaatatc atcaaaaaaa gacaacttct cttgtatggc tttaatcaaa
      721 aaactattgt cgtcaatgat agcaagtttg atattcatag ggttaaaatt aagataaatt
      781 agtagttgat atgagatgca aaaattcaag tttcaattgg attgctgtca acatattttg
      841 agtattttct tctattatgg cattttcatg agtcaaatca tttctttcat ttttaatgtt
      901 ttgctccttg tttatttgat aaagcagatg caacattatt ttttgatgtt ctttaacttg
      961 tgcattggtt ttatttttaa aataaactat caatagtgta taaattaaaa ataacaagcc
     1021 taaaataaat tgattcataa tttccccaaa ttgaacttca aatttaattt ataattaaag
     1081 actgttccat acggcatttg ccttatattg ttaaattgga tattttatca acactgttgt
     1141 cccttgattt tcttttgaat ctataattat ttcagctcct atttccgaag ctcttttttg
     1201 catattattt aaaccattcc ctaaatgaac ttctgctact gaaaaccctt ttccatcatc
     1261 agtaatatta aaaaacaata ctgaattttc ttctttcaaa tgcacaataa tttgttttgc
     1321 ttgagcatat tttacagcat tatttaccgc ttcttgaata attctataca cattcattcc
     1381 ttccaccgaa gtaaattcat gagctgaatt gacattcggg tcaatactaa attcaaatgt
     1441 aataccatga acagctaatt gagcattatc aataaaatta gtgatcctga tttgtaaatc
     1501 ttccactgaa attgaggatt tattcattgc ccaaatggta tcacgtaatt ctgtgatggt
     1561 atttcgggta aaaccactga tattgtcaaa ttttgaaacc aatttttctt tggcaatatc
     1621 aaaatatttt aaattgtcaa tggatgaaat aataaaagtc aattgtgaac cgatgttatc
     1681 gtgtaaatct cttgaaattt gtagtcgctg ctcttgtaac ttattttggg tttcaatttt
     1741 taccaatgct tcttttaatt caacttcttt gagtagttgt atatttttaa ttttttgttg
     1801 attatagaaa agataaccta ctaatcccag caaaatggca aacccgactg agccgtaaat
     1861 aattgtgttt ttttgtttta tttgcagttc attctctgca atttctgctt tgttttttga
     1921 aatttcagca tctttttgag ccgatttata tttcacttct aattcatgaa gattttctaa
     1981 attagaattt ttaaaaacac tgtctttcgc tgtaaaaaac tttttgttat aatcatttgc
     2041 tgttttataa tccgataatg cttcataata taaggcataa ttttctaaag cattcacata
     2101 atcttctgct acttttgatt tttttgcata ataaaaagca ctgtctatat aatttttagc
     2161 acttgcattt cttttaaatt ccagcattac gagtgccaaa ttattataac tggcagctac
     2221 tcccggaaaa tttttaattt ctcgttctaa ttcaactgat ttttcaaaat aatatttggc
     2281 tgaatctatt tgttgtaaat aataataaat tcctgctaca ttattatatg attcggcaat
     2341 tcctttttta tcttttaatt tttcctctat ttggagagaa taattaaaat aacgcaatgc
     2401 ttttgtcaaa ctgtcttggt tggctaaagc gattgctaag ttattatagg attccgaaag
     2461 tcctttttga tacttcgatt tttgaaatat agcaattgct tttcgatgat atacttttgc
     2521 cttttcgagc agatccatct gactgtaaat catgccaatg ttattgatta ctgttccttg
     2581 ttgatcaata tccttttttg attcatatat ttttaaagca atcaaataat tctttagtcc
     2641 ttcttcataa aaccccaatg atcttttaca ggctccaatg gtattgttgc aagaagcaat
     2701 taaaagtgaa tcatttgata attttaaggc tttctctgca tattttatag cttctttaaa
     2761 ttcccctttt aaacgaaaaa gaacagcata attactatat gtgacagaca actccttttt
     2821 attttcaagt tgcaatgctt ttttttctgc tgattttact agctgcatgg ctttttcggg
     2881 ttccgttctg tgaatgtcca tggctttttc gttcaattgc ttagctgaat aggtctcaaa
     2941 agactgacca tttgccaatt gaacaaaaaa taaaatcaga aaaacatatt ttaatccaaa
     3001 ccccttttca attatttttt tcattgtatc aactattaaa atgatttaaa gacaatttaa
     3061 ctaaactgcc tttttcaagg gttataaaat caatttctgc ccctagttcc tgagctcgtt
     3121 ttctacaatt agaaagtcca ttaccttcta ttatttcgtt caataaaaac cctttaccat
     3181 tatcacttat ttcaattatt aatgtattgt tggcgatata aatttgaatt tttatggaag
     3241 tacatccgga atgcttaatg gaattattaa gagtttcttg tataattcta taaatattca
     3301 ttccttccac agatgaaaat ttaaggttgc ttgggcattt tttgtctatt gaaaattcaa
     3361 aagcaattcc atgtgtagcc actttagcct tttcaataaa attaataatc cttatttgca
     3421 aatcttcaag tgtaatttca tttttattca tggcccaaat cgtatccctc aattcggtga
     3481 ttgtattttg agtaaaccca ctaatcgcat caaattttga aaccaattta tctttagcaa
     3541 tgtcaaaata ttttaaattg tcaatggaag aaatgataaa cgttaattgg gaaccgatgt
     3601 tatcatgcaa atctctggaa atttggagtc tttgttcctg caacttattt tgagtttcaa
     3661 ttttaaccaa ggcttctttc aattcatttt ctttgataag ctgtgtgttt tttaattttt
     3721 gttggttgta aaacaaaaaa ccaattaata agaaagtgac agaggcacca attgacaaat
     3781 acaattgcgt atttttttgt tttatttcta gttctctttc tgccactttt actttagccc
     3841 tggctaaatc tttttctttt ttttcaactt gatatttagt ttcaatctct gaaactgctt
     3901 tttgtacttc taatccaata attacatttt gcaatgaatc tttacttact tgatttaaat
     3961 aagctgcctt aaaatcattt tgtaatagtg ctaattgtat ttcgccataa taataatttt
     4021 ttaaaccggc ctgatcttcc gtctcaatac aatatttttt ggctaattca ttcgcttctt
     4081 ttgcttttac tatatctttt tttgccctgt aaatatccgt taaacctagg taagcataag
     4141 ctagaatgga tggtgcttca agttgttccc caatttgaat ggcctcaagg caggaagaat
     4201 aagcttctgc tattttacgc tcattgtatt gtaaggacga taaattattc agtgtaatgg
     4261 cgatgcctct taaatcattt atttttcttt tattagaaat tgctttttca taataaaaaa
     4321 tcgctttttc attttcattt gcatcttcat gaaatcgacc caaatttgta taactcacag
     4381 atataccata ataatcatga atttcttctc ttaatttcaa cgattgaaaa aaatattttt
     4441 cagcatttat tttatcattc aaactttgat aaataagtcc gatattactc tgggtgttgg
     4501 cttgcccttt tttatcatta attttttcaa aaatttgcaa acctaaatta taatatttca
     4561 gagcatttag ataatttgct ttattccatt ctaataatcc aatgttgttg aatgcggatg
     4621 cttgagtggc aaataatttt gcttttttag aatggtaaat agctttttga taacagtata
     4681 atgcactgtc tgattttcca ttgatatcaa aaattatccc ttttatattg aatgatttag
     4741 ctaggccaaa atcgaggtat tttctttttg aaagtgtaaa agattgccta ttcaatttat
     4801 tcgcttccgt taaatttgag ctcaaaaata tatttgacaa tttatacaat gcatcaattt
     4861 taatagaatc atttttttga tttctaacaa cttgatacaa gctatctaca ttttgtgaaa
     4921 ataaaagttg tgtaaggaaa aaaaataaaa tacaataaat gactctcatc caataaatca
     4981 taaaaaatta tgtactaaat taataattta atggttacta aaactagagc agtaaattat
     5041 taaatccttt tgtataagaa tttgataaaa acaaaccact ctccataaaa aaaagtaagt
     5101 ggtcaatcaa aaaatgcagt gaataataat ttattaccta ttttaaaatt tgtattaaat
     5161 tcagcaacaa taccaaaaag gcaagaaggt aaagcagtag agctactcgt aagggtttga
     5221 cagttttcat agtacgcatt tttatcggtt agtacaaagt tgaactattt ttgattgcta
     5281 cacaatacgt catttgacgt ataaaaaact tcccaaactt ggtttaaaag tttgagaagt
     5341 actaaaaaat ttactaaaat ttaatcaaat cattctaagt ccataagtca gaattaaaca
     5401 aaacttcttt ttattgacgt gtgatggagt taatctttaa ttttccgtca gtgatgactt
     5461 tatcaacaga tgaattattt cctgtaaaat taaatgttgc ttcaatttta ttggctacgg
     5521 tatcacaaac tgttaccgtt aatgtacctg aagttgaatg aaagtaatta tccggataat
     5581 tgacgccatt ttcataagtc aaccaacaag atgccatatc gaacgattgt tgagcaggaa
     5641 aacttcctgt tttagcagga aaatgaaatt ctgcaaaatc atttccgttt ttacttccat
     5701 aaacatcaat ataccttccg ccggctactg catttgtata taaaactgct tcaaactcat
     5761 cggctacaat tgaggttcca tctaccttaa aactcaactc ttcctgagcg gttgaaccac
     5821 catcattatc atcgttacta caactgaaaa atccaattgt tatcatcaca agtcctattt
     5881 taaatatatt ttttttcatt gtttcttaaa tttattagtt agaaaaaatt cctctaaagc
     5941 ttccgttagt aatacttact gttcctccat tacacgtttc gtccgctttc acttctttgc
     6001 ccacaaaact aaatgttcct tctacttttg tttcatcaat ataagtgatt tcgattgtac
     6061 cggtcgcatt ttcgcaattc acagaattat atgctactgc cgacatattt actaatttca
     6121 tgaagttaca gcttccaaaa ttgacatcaa aattgtttgt tgcattgaca ttataaacac
     6181 ctgtttctaa actatgatct attgctccca agtttaacga aatagttgtg ccatccatta
     6241 acgaaccttg aattactaaa tttccggttg aataggccga aacagttaac tgttccgttg
     6301 attgaaaatc ggcaccatca atggatgctt ttacataact tccacttgga agagctgaac
     6361 ctccaccatc gtcactagaa cacgaagtga aaattgtagc agtcaataaa actaaaacac
     6421 tgagttttga aattgttttc attattattc ttttatttaa gattagtaaa gcaaaattgc
     6481 attgtatcgc cttttcatac aatacgacaa atgccctatt tttaactttt cattaaatct
     6541 gactttggaa tagtcacctg ctttaaatat tgta
//
LOCUS       DAOTCW010000124         6518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_8667_length_6518_cov_51.196039, whole genome shotgun sequence.
ACCESSION   DAOTCW010000124 DAOTCW010000000
VERSION     DAOTCW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8667_length_6518_cov_51.196039"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            551..2236
                     /locus_tag="P5335_09360"
     CDS             551..2236
                     /locus_tag="P5335_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRZ75126.1"
                     /translation="MMSEEKSLNFIEQIIEEDLKNGFPKEKLRFRFPPEPNGYLHVGH
                     ASAICLNFGLGIEYQSPVNLRFDDTNPAKEEQEFVDAIKRDVQWLGFQWDEERFASDY
                     FQELYDWAVEFIKKDKAYIDSQSSEDMAKQKGTPTQVGTNSPFRNRSIEENLDLFERM
                     KNGEFPEGTHVLRAKIDMASTNMLMRDPIMYRILHKHHHRTGDTWCIYPMYDWAHGES
                     DYIEGISHSLCTLEFLPHRELYDWFLDQIYDTSKVRPKQREFARRNLSHTVVSKRKLL
                     QLVQEGHVTAWDDPRMSTISGMRRRGYTPASIRNFAKTIGIAKRTNLIDVSLLEFCVR
                     EDLNKTAPRVMSVLNPVKLIITNYPEGKEEWLEAENNPEEEIMTFRKLPFSKELYIER
                     EDFQEEANKKFFRLTLGTEVRLKNAYIIKGENVIKDADGNITEIHATYDLDSKSGSGT
                     EASQRKVKGTIHWVSIPHAIKAEVRLYDRLFTHENPDGDKEVDFKEYINPNSLEVITG
                     FVEPSLQLAQPEDKFQFQRLGYFCVDKDSSAEKIVFNKTVGLRDTWAKVEAKE"
     assembly_gap    2240..2339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2340..2675
                     /locus_tag="P5335_09365"
     CDS             <2340..2675
                     /locus_tag="P5335_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRZ75127.1"
                     /translation="KIMSNRTGDTFMALLIGAAIGVGVGILFAPDKGATTRQKIKETF
                     DESGDDVVEKFNDIVSKIQNKAADTKISFEKTLEDLVSEGSYKTEELITLLEQKLKAL
                     KEENAKYQK"
     gene            2679..3029
                     /locus_tag="P5335_09370"
     CDS             2679..3029
                     /locus_tag="P5335_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein"
                     /protein_id="HRZ75128.1"
                     /translation="MAFEEIKENVNQIQEETKAYLESSAAYYKLWGFKVAMKSASTIV
                     KFSLIAICLSMVLLFSSIAGAIALGYALDNFAYGFLIVSGIYLLITLALFLLKDKIVE
                     GPVMAKFSEIFFND"
     gene            3032..3277
                     /locus_tag="P5335_09375"
     CDS             3032..3277
                     /locus_tag="P5335_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6327 family protein"
                     /protein_id="HRZ75129.1"
                     /translation="MESKLYNTFEEIERDLQILKLEREIHHKKIILHIEQTKDSLTPR
                     NLLSGLLHFSIPKNLGSIIKLFAPLLIQWFFNKKRGS"
     gene            complement(3290..4258)
                     /locus_tag="P5335_09380"
     CDS             complement(3290..4258)
                     /locus_tag="P5335_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRZ75130.1"
                     /translation="MIPTYIIIVLAVFLLLASFFTVKQQTAAVIERFGKFSSTRQSGL
                     QLKIPVIDRVAGRLNLKIQQLDVIIETKTKDNVFVKMKVSVQFKVIQEKVYDAFYKLE
                     YPHDQITSYVFDVVRAEVPKLKLDDVFERKDDIAVAVKRELNEAMTTYGYDIINTLIT
                     DIDPDIQVKNAMNRINAADREKTAAEYEAEAGRIRIVAKAKAEAESKRLQGQGIADQR
                     REIARGLVESVDVLNRVGINSQEASALIVVTQHYDTLQAIGADANSNLILLPNSPQAG
                     SDMLNNMVASFTASNQVGEAMKKGAIKKANKKVEPKDEFGTTEENQ"
     gene            4333..4989
                     /locus_tag="P5335_09385"
     CDS             4333..4989
                     /locus_tag="P5335_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PorT family protein"
                     /protein_id="HRZ75131.1"
                     /translation="MKRYFYTLILLGFTNAVLAQYGRGDYNHIGLSLGINQTSLYTDN
                     FNITPKTSWTGGLAMRGNYYNSFAMVYGMNFTENKFSIETNSSSATKEVDLKMMAVQI
                     HLLLSYRIAESAFSIDFGPVLQVNSELKFDEADENNFITDNDLLQVKDLEKITPINFN
                     VQVGVTGGFENVRLNLCYQYGVTNILNNLNDQDEVALKADGKLKGNLGVFAGNIIFYF
                     "
     gene            5009..6406
                     /locus_tag="P5335_09390"
     CDS             5009..6406
                     /locus_tag="P5335_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75132.1"
                     /translation="MKKFILAILLMASFYCTSQTVIVDEKFQKDDVPLAYISLSNNFI
                     IQKGKHDGVSRNREVHELSSYDSKAVKTKLLENVKVMNIRFSPSGNTFKTDEFEKMSF
                     SGDHKYYKNGKKIADLPKTNKFSLILDDFLFNIGNKLDGNDLSFTITDLPSFKTHTKK
                     YESPGLDRIVNENNVEYARDVVTSSRASNDYFELISKSISKDYKTTTLFRTRYNYDGK
                     KISDIGYKIDIREDFLIYSNNSGGEIEIKGSGDTAKRIFSDDLSINNFKVDPITNEVY
                     VYGLYGKRAEKSNDNSNTPLGYYVFKFKEDGTKIWEVINAIDDKGDFTDSQNMTYLYV
                     DLFLMGESVVFSAGAKESLDDYIHYIKLNSIGKVDSTSKITFKSESSAYNISSYGDKK
                     FLHANLTNKEDLKNKNLDYKTIIVYNTNQKIKEYIKTVNNKNKTSFCATVYKESVWLV
                     ESDNETYYKVTYFEN"
     gene            complement(6441..6515)
                     /locus_tag="P5335_09395"
     CDS             complement(6441..6515)
                     /locus_tag="P5335_09395"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75133.1"
                     /translation="MFDIIELIGKEETIKRLKKAITSL"
BASE COUNT         2253 a          932 c         1116 g         2117 t
ORIGIN      
        1 gtaaaactaa ggttgtaagt attaaatcct tgtgaggcac aagcttgagt gaatcctaaa
       61 gtgggcactc caattgttat agtttgagca tacccattgc cgttgctact aaatagcaaa
      121 gaaagcaaag cagaaaaaaa cagttttttc ggggtaattt tccaaaccat aaaacactct
      181 ttatgattga ttgcttttac cccaaaagca accgtattgt tattttgtaa ttgaaatagg
      241 cactacacta catttaatta cgaaaaaaac gataataagt ttttactatt tttagtaata
      301 aattctgttt ttaacatttt tctgtttcga atttagggaa taataaacat catattcaaa
      361 actttaacga taaaatcgat gaatgacacg caagtttgtg gtaaaccgta attggctctg
      421 ttgaaaaccg taacggtaaa tgtcgttcgt cgattaatta aaaaagatag tgtatttcat
      481 cggtattttt tttaaaaatc attttcggag gttcggtgtt tttttccgta tttttgtcgc
      541 tcatttattg attatgtcag aagaaaagtc actcaatttt attgaacaaa taatcgaaga
      601 agatttaaaa aatggttttc caaaagaaaa gcttcgtttt cgctttccac cggaaccaaa
      661 tggctacctt catgtgggtc atgccagtgc tatttgttta aatttcggat taggaattga
      721 atatcaatcg cccgtgaatt tgcgttttga tgataccaat cccgctaaag aagaacagga
      781 atttgttgat gccatcaaac gtgatgtgca atggctagga tttcagtggg acgaagaacg
      841 ttttgcgtcc gattattttc aggaattata cgattgggct gtcgagttta ttaaaaaaga
      901 caaagcctat atcgacagcc aatcttcaga agatatggcc aagcaaaaag gaacacctac
      961 acaagtagga acaaatagtc catttagaaa tcgttcaatc gaagaaaatt tagacttatt
     1021 tgaacgcatg aaaaatggtg aatttcctga aggaacgcat gttttacgtg ccaaaattga
     1081 tatggcttca actaatatgt tgatgcgtga tccgattatg tatcgaattt tgcataaaca
     1141 tcaccataga acaggagata catggtgtat ttatccaatg tatgattggg ctcatggaga
     1201 aagtgattat atagaaggta tttcacattc actatgtaca ttagaatttt tgccgcacag
     1261 agaattatat gattggtttt tagatcaaat atatgacact tcaaaagttc gtccgaaaca
     1321 aagagaattt gccagaagaa atctttcgca tacggtagta agtaaacgaa aattgttgca
     1381 attggttcaa gaaggtcacg ttacagcatg ggatgatccg cgcatgagta cgatttccgg
     1441 tatgagaaga agaggttata ccccggcttc catccgaaat tttgctaaaa ccattggaat
     1501 tgcaaaacga acaaacttaa tagacgtttc attattagaa ttttgcgttc gtgaagattt
     1561 gaataaaacg gcacctagag taatgtctgt tttgaatccg gtaaaactta tcatcacgaa
     1621 ttatcctgaa ggaaaagaag aatggttgga agctgaaaat aatcctgaag aagaaataat
     1681 gacgtttaga aaacttcctt tttcgaaaga attatacatt gaaagagaag attttcaaga
     1741 agaagcaaac aaaaaattct tccgattgac tttaggaaca gaggttcgat tgaaaaatgc
     1801 ttacattatt aaaggtgaaa atgtcatcaa agatgctgat ggaaatatta ccgaaattca
     1861 cgctacttat gatttggatt ctaagtccgg aagcggaaca gaagcaagtc aacgtaaagt
     1921 aaaaggaacg attcattggg tttcaattcc acatgccatt aaagcggaag ttcgtctata
     1981 tgatcgattg tttacccatg aaaatccgga tggagacaaa gaggttgatt ttaaagaata
     2041 tattaatcca aattcgttag aagtgattac cggttttgtt gaaccgagtt tacaattggc
     2101 tcaacctgaa gataaatttc agtttcaacg attaggttat ttttgtgtgg ataaagattc
     2161 ttccgcagaa aaaattgttt ttaataaaac ggtcggttta agagatactt gggcaaaagt
     2221 ggaagcgaaa gagtaaaatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     2341 aaattatgtc aaatagaaca ggagatactt ttatggcact tttaattgga gctgctattg
     2401 gagttggagt aggaattttg tttgctcctg ataaaggagc aactaccaga caaaaaatta
     2461 aagagacttt tgatgaatcc ggagatgatg ttgtagaaaa attcaacgac attgtttcaa
     2521 aaattcaaaa caaagcagct gacacaaaaa tttcttttga gaaaacatta gaagatttag
     2581 tttctgaagg aagttataaa acagaagaat tgataactct tttggaacaa aagcttaaag
     2641 ctttaaaaga agaaaacgct aaatatcaaa aataaacaat ggcttttgaa gaaataaaag
     2701 agaatgtaaa tcaaatccaa gaggaaacca aagcttattt agagagcagt gcggcttatt
     2761 ataaactttg gggttttaag gttgccatga aatcggcttc aaccatagta aaattttcac
     2821 ttatagccat ttgtctgtca atggttttat tgtttagttc tattgccgga gcaattgcat
     2881 tggggtatgc attggataat tttgcttatg gttttttaat tgtaagtggg atttacttac
     2941 taattacgct tgctcttttt ttactaaaag ataagattgt ggaaggtcct gtgatggcaa
     3001 aattttctga aatctttttt aacgattaag tatggaatct aaactatata acacatttga
     3061 agaaattgaa cgggatttac agattctcaa gttagaacgt gaaattcatc ataaaaagat
     3121 aattttacat attgaacaaa ccaaagatag tctcacgccc agaaatcttt tatccggatt
     3181 attacatttt agtattccaa aaaacttggg aagcattatc aaactatttg caccattgct
     3241 tattcaatgg tttttcaata aaaaaagggg ctcgtaagcc ccttttgaat tattgatttt
     3301 cttcggttgt tccaaattcg tcttttggtt caactttttt gtttgctttt ttgatggctc
     3361 cttttttcat agcctctccc acttgattac ttgccgtaaa actggcaacc atgttattca
     3421 acatgtcact accggcttgt ggtgagtttg gtaacaaaat taagtttgaa ttagcatctg
     3481 ctccaatggc ttgtaatgta tcataatgtt gcgtaaccac aatcaatgct gaagcttctt
     3541 gtgaattaat ccccacacga tttagaacat ctacactttc tactaaacct cttgcaattt
     3601 ctcttcgttg atcggcaata ccttgacctt gaagcctttt gctttctgct tctgctttag
     3661 ctttagctac gattctgatt ctaccggctt ccgcttcata ttctgccgct gttttttcac
     3721 ggtcagcagc gttgattctg ttcatggcat ttttcacctg aatgtccgga tcaatatcgg
     3781 taatcaacgt gttgataata tcataaccat aagtagtcat tgcttcgttt agctcacgtt
     3841 taacggctac agcaatatca tccttacgtt caaaaacatc atctaatttt aattttggaa
     3901 cctccgcacg caccacatca aatacatacg aagtgatttg atcatgtgga tattctaatt
     3961 tgtaaaaagc atcatatact ttttcctgaa taactttaaa ttgaacagaa actttcattt
     4021 tcacaaatac gttatctttc gttttagtct caatgatgac gtctaattgt tgaattttaa
     4081 gatttaatct tccagctact ctgtcaatta ctggaatttt aagttgcagt ccggattgtc
     4141 tcgtactact gaatttacca aaacgctcaa ttactgccgc tgtttgctgt tttacagtaa
     4201 aaaaagaggc caatagtaaa aatacagcca atacaataat aatgtaagtg ggaatcataa
     4261 aaaatagttt tatttgttat atgtaattat acgaaaattc ccgtacgttt gttacaactt
     4321 aaagtactat tcatgaaaag atatttttat acattaattt tattggggtt tactaacgct
     4381 gttttggcac aatatggaag aggagattac aaccatattg gattaagcct tggaataaac
     4441 caaacgagtt tatatactga taattttaat attacaccta aaacaagttg gaccggaggt
     4501 ttagcgatgc gagggaatta ttataatagt tttgccatgg tttatggtat gaattttacg
     4561 gaaaataaat tttcgataga aaccaattct tcatcggcaa ccaaagaggt agatttaaaa
     4621 atgatggcgg ttcaaattca tttattgtta agttatagaa tagcagaaag tgcattttcc
     4681 atcgattttg gtccagtact gcaagttaac agtgaactga aatttgatga ggccgatgaa
     4741 aataatttta tcaccgacaa tgatttgttg caagtaaaag atttggaaaa gattacacca
     4801 ataaatttta atgtccaagt aggagttacg ggcggatttg aaaatgttcg attgaattta
     4861 tgttatcaat acggcgtgac taatattttg aataatctca acgatcaaga tgaagttgct
     4921 cttaaagcag atggtaaatt aaaagggaat ttgggtgttt ttgccggtaa tattatcttt
     4981 tatttttaag caaaattatt ctacaattat gaagaaattt attttagcca ttttattgat
     5041 ggcttctttt tattgcacaa gtcaaacggt tattgttgat gaaaaatttc agaaagacga
     5101 tgtcccgttg gcatatattt ctttatcaaa taattttatt atccaaaaag gaaaacatga
     5161 tggcgtttct agaaatagag aagttcatga attgtcatct tatgactcta aagcagttaa
     5221 aacaaaactt ttagaaaatg taaaagtgat gaatattcgt ttttctccat ctggaaatac
     5281 atttaaaact gatgagttcg agaaaatgag tttttcggga gatcataaat attacaaaaa
     5341 cggaaaaaag atagcagatt taccaaagac taataaattc agtcttattt tagatgattt
     5401 tttatttaat attggtaata aattagatgg taatgattta agttttacaa ttacagattt
     5461 accttctttt aaaacacata caaaaaagta tgaatctcca ggtcttgata gaatagtcaa
     5521 tgaaaacaat gttgaatatg ctagagatgt tgttacaagt tcaagagcat caaacgatta
     5581 ttttgagtta atttcaaaat caatttcaaa agactataag acaactactt tattcagaac
     5641 aagatataat tatgatggaa agaaaataag cgatattgga tataaaattg atattagaga
     5701 agatttttta atttactcaa ataatagcgg aggagaaatt gaaattaaag gatctggcga
     5761 tacagctaaa cgtatttttt cagatgattt aagcatcaac aactttaaag ttgaccctat
     5821 tacaaatgaa gtttatgttt acggattata tggtaaaaga gctgaaaaat caaatgataa
     5881 ttctaataca cctttaggtt attatgtttt taaatttaaa gaagacggca caaaaatttg
     5941 ggaggttata aatgctatag atgacaaagg tgattttaca gatagtcaaa acatgactta
     6001 tctttacgta gatttatttt taatgggtga atcagttgtt tttagtgcag gagcaaagga
     6061 atcattagat gattatattc attatataaa attaaattct attggaaaag ttgattcaac
     6121 ttctaaaata actttcaaat cagaatctag tgcttataac atttcaagtt atggagacaa
     6181 aaaatttctt catgcaaatc ttactaataa agaagatttg aagaataaaa atttagatta
     6241 caaaaccatt attgtttata atacaaacca aaaaattaaa gaatatataa aaacagttaa
     6301 caataaaaat aaaacatctt tttgtgctac tgtatacaaa gaaagtgttt ggctagttga
     6361 atcagataat gaaacatatt acaaagtaac ttacttcgaa aactaaagaa aaagcactga
     6421 atttcagtgc tttttctttt ttataaacta gtaatcgctt ttttcaatcg cttgattgtt
     6481 tcctcttttc caatcaattc aataatatca aacaaatg
//
LOCUS       DAOTCW010000125         6500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_8695_length_6500_cov_47.990846, whole genome shotgun sequence.
ACCESSION   DAOTCW010000125 DAOTCW010000000
VERSION     DAOTCW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8695_length_6500_cov_47.990846"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="P5335_09400"
     CDS             complement(<1..251)
                     /locus_tag="P5335_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRZ75134.1"
                     /translation="MKKVGNPNSISRILQFSLALMLTFTFQLFAQDAASAAAPATADA
                     APAAVVSGDPAAGKALFNAQCAACHKLDAKMTGPALRGVG"
     gene            471..860
                     /locus_tag="P5335_09405"
     CDS             471..860
                     /locus_tag="P5335_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRZ75135.1"
                     /translation="MLFQERKKCLYTLILLTLFVSNFTLSAQNITVEQDAKFEQLLNE
                     KRKVNASIAMNDRWKIQIFTGDNENSKKTLLAFKKENKNIDCSIFFHTPVYKVWVGNF
                     KTRIEAERNLKDIKLKYPNAFLIKPNK"
     gene            complement(914..3715)
                     /gene="infB"
                     /locus_tag="P5335_09410"
     CDS             complement(914..3715)
                     /gene="infB"
                     /locus_tag="P5335_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRZ75136.1"
                     /translation="MSEEKNIRINKVLRELNISLERAVDYLKDKGIAIEASPNTKISE
                     DVYATLCNQFAGDKGNKEASKEVSEEKRKEKEALRIEREKELELLKKQEEDLHRQQDV
                     IKAKAVVSVPVQVGKIDLEPKKVVVPQPEKIVEVKPTPVAEVVKPEPIQPKPIVQEEK
                     VKIEPKKIEPKVIVPKVEEPKKAEPKVATDIPVVDEKFETQYQKLSGTTTTGQKIDLS
                     QFDKPKKKKEEPKIQPRSATQGAASDANKNKRKRIPPKPGTPGGGQNQGNQQGGGAIR
                     PNQPIRPGFNKGVRPAIVAKVEPTEEEVQKQIRETLEKLQGKGNKSKAAKYRRDKRDT
                     HRQRSDDELKAIEEGSKTIKVTEFVTVGEIATMMDVPITKVIGTCMSLGIMVTMNQRL
                     DAETLSIVADEFGYEVEFITTDIEDAIQVVEDKEEDLVHRAPIVTVMGHVDHGKTSLL
                     DYIRKTNVIAGESGGITQHIGAYSVTLENGQKITFLDTPGHEAFTAMRARGAQLTDIA
                     IIVVAADDDIMPQTKEAISHAQAAGVPMIFAINKVDKPNSNPEKIKERLAGMNLLVED
                     WGGKIQSHDISAKVGTGVKELLEKVLLEAELLDLKANPNKPAVGTVVEAFLDKGKGYI
                     STVLVQAGTVKIGDYVLAGKNHGKVKAMHDERGNSLKEAGPSTPISILGLDGAPTAGD
                     KFNVFEDEREAKQIAAKRSQLMREQSVRTQKHITLAEIGRRIALGQFKELNIILKGDV
                     DGSVEALSDSFSKLSTEEIQIKIIHKGVGAITESDVLLASASDAIIIGFNVRPAGNAK
                     QLADKEEIDIKTYSIIYDAIDDLKDAMEGMLSPEMKEEISGTAEIRETFKISKIGTIA
                     GCMVLDGKIIRSSKIRLIREGVVIYTGDLVALKRFKDDVKEVSKGYDCGIQIKGYNDI
                     EINDLIECFSQVEVKKKLK"
     gene            complement(3751..4995)
                     /gene="nusA"
                     /locus_tag="P5335_09415"
     CDS             complement(3751..4995)
                     /gene="nusA"
                     /locus_tag="P5335_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRZ75137.1"
                     /translation="MENIALIESFSEFKDDKLIDRVTLMAILEDVFRNALKKKYGSDD
                     NFDIIINPDKGDMEIWRNRIVVADGEVEDENSEISLSEARKIEPDFEVGEDVSEEVKL
                     IDLGRRAILALRQNLISKIHEHDSTNLYKQFKDLIGEIYTAEVHHVRPRVVILVDDDG
                     NEIVLPKENQIPSDFFRKGDNVRGIIENVELKGNKPQIIMSRTSEKFLEKLFEQEIPE
                     IFDGLIMIKKVVRIPGEKAKVAVDSYDDRIDPVGACVGMKGSRIHGIVRELGNENIDV
                     INYTSNIQLFITRALSPAKVSSIKIDEEKKRAEVYLKLEEVSKAIGRGGHNIKLAGLL
                     TGYELDVIREGSVAEEEDDVELTEFSDEIDGWVIDEFAKIGLDTARSILKHSVEDLVR
                     RTDLEEETVQDVMRILKSEFEG"
     gene            complement(5012..5476)
                     /gene="rimP"
                     /locus_tag="P5335_09420"
     CDS             complement(5012..5476)
                     /gene="rimP"
                     /locus_tag="P5335_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044047587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HRZ75138.1"
                     /translation="MTFKEKVVALLNDGLAEKPAVFLIDLTITDSQKIIVTLDGDDGV
                     TLQDCIDISRAIENNLDREEHDFSLEVASAGVSTPLKMVRQYKKNIGRVLKIKTAKDT
                     IEAKLVAVNDDFITLTWSAREPKKIGKGKETVEKNLELPYKEIKEAIVTITF"
     gene            5655..6479
                     /locus_tag="P5335_09425"
     CDS             5655..6479
                     /locus_tag="P5335_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRZ75139.1"
                     /translation="MKKILIPTDFSEHAEYALKVAAQIAKKHDGEIILLHMLELPNQT
                     GDAVSSGHALPEIMFFKNAALAKLESLMDSDFLEGLKVSEIVQFELAFDGILKLIKKN
                     DIDLIVMGSHGASGFKDMFIGSNAEKVVRHSEVPVLIIKKEIPDFTINKFVFASDFSD
                     EVKVPFAKVVEVANKFEAELNLVMINTPNSFKSTLVAQEIMDKFVSQFNIKKVSTHIF
                     NDANVEKGILNFSNYINADLIGMCTHGRKGLSHFFNGSISEDLVNHAVKPVLTFKI"
BASE COUNT         1951 a         1238 c          906 g         2405 t
ORIGIN      
        1 ccaacacctc ttaaggcagg acctgtcatt tttgcatcta atttgtgaca tgcagcacat
       61 tgggcattaa acaatgcctt accagctgca ggatcacctg aaacaactgc agcaggagcg
      121 gcatcggcag tagccggagc agcagctgaa gcagcgtctt gagcaaaaag ttgaaatgta
      181 aaggttagca ttaatgctaa actgaattgt agaattcttg aaatcgaatt agggttaccc
      241 acctttttca tataatagaa tataatttgt cgacaaattt tggtatgatt ttttctatag
      301 ttcttaataa aaacaactgt atttatttta aactcggaca aaaatacgat ttatgagcta
      361 ttctcaaaac cttaaaatac tcttaaatcg taatttatat ttattctaaa taattttttt
      421 gtttttgttt tatttcataa cgcgtacatt tgtattaaaa tcaattcatt atgctatttc
      481 aagaaagaaa aaaatgcctt tacacgttga ttctattaac tttatttgtt tcaaatttca
      541 cactcagtgc acaaaacatt acagtggaac aagatgctaa atttgaacaa ctgttaaatg
      601 aaaaaagaaa agttaatgcc tctattgcaa tgaacgatag atggaaaatt caaattttca
      661 cgggggataa tgaaaattca aaaaaaacgt tattagcttt taaaaaagaa aacaaaaaca
      721 tagattgttc tatctttttt cataccccgg tttataaagt ttgggttgga aattttaaaa
      781 caagaataga agctgaacga aatttaaaag acataaaatt aaaataccct aatgcttttt
      841 taatcaaacc taacaaatag aattgcatct tattaaataa aaaatcccga ttgctcggga
      901 ttttttattt aatttatttt agttttttct tcacttcgac ttggctgaag cattcgatta
      961 aatcgttaat ttcaatgtcg ttgtatcctt taatttgaat accacaatcg tagcctttag
     1021 aaacttcttt tacatcatct ttaaatcgtt ttaaggcaac taaatctcca gtgtaaataa
     1081 caactccctc tctaatcaat ctgattttag aacttctaat aattttacca tctaaaacca
     1141 tacatcctgc aatagtacca attttagaaa ttttaaaggt ttcgcgtatt tctgctgttc
     1201 ctgaaatttc ttctttcatt tccggagata acattccttc catagcatct ttcaaatcat
     1261 caattgcatc ataaataata gaatatgttt tgatgtcaat ctcttcttta tctgccaatt
     1321 gttttgcatt tccggcagga cgaacattaa atccgataat aatcgcatcc gatgcagaag
     1381 ctaacaaaac atccgattct gtaatggctc caaccccttt atgaataatt ttaatttgaa
     1441 tttcttcagt agataattta gagaaggaat ctgaaagtgc ttcaacagat ccatccacgt
     1501 caccttttaa aatgatattt aactctttaa attgtcctaa agcaatacgt cttccaattt
     1561 cagcaagtgt aatatgtttt tgtgttctaa ccgattgttc acgcatcaat tgagaacgtt
     1621 ttgcagcaat ttgtttcgct tctctttcgt cttcaaatac attaaactta tctcctgctg
     1681 ttggagcacc atctaaacct aaaattgaaa ttggtgtaga aggaccggct tcttttaaac
     1741 tgtttccacg ttcatcatgc atagctttta ctttaccatg attttttcca gctaaaacat
     1801 aatctccaat tttaaccgtt cctgcttgta ccaaaacggt agagatataa ccttttcctt
     1861 tatcaaggaa agcctccaca acagtaccaa cagctggttt attaggattt gctttcaagt
     1921 ctaacaactc agcttctaat aatactttct ccaataattc tttaacaccg gttccaactt
     1981 ttgcagagat atcatgtgat tgaattttac caccccaatc ttcaaccagt aaattcatac
     2041 ctgctaatct ttctttaatt ttttccggat tagagtttgg tttatctact ttgttaatcg
     2101 caaaaatcat tggcacgcca gccgcttgag catgactgat tgcttctttc gtttgtggca
     2161 tgatgtcatc atccgcagct actacaataa ttgcaatatc cgttaattga gcaccacgag
     2221 cacgcattgc tgtaaacgct tcgtgaccag gtgtatctaa aaatgtgatt ttttgtccat
     2281 tttcaagtgt tacactataa gcaccaatgt gttgtgtaat tcctcccgac tcgccagcta
     2341 ttacatttgt tttacgaatg taatccaata aggacgtttt accgtgatcg acgtgaccca
     2401 ttactgtaac gataggagct ctatgtacta aatcttcttc tttatcttca acaacttgta
     2461 ttgcatcttc aatatctgta gtaataaact ctacttcata accaaactca tcggcaacaa
     2521 ttgacaatgt ttctgcatct aaacgttggt tcatcgtaac cataattccc aaagacatac
     2581 aagttccgat aactttggta attggcacat ccatcatcgt agcgatttct ccaacagtaa
     2641 caaactccgt tactttaatg gttttgcttc cttcttcgat tgcttttaac tcatcgtcag
     2701 agcgttgacg gtgtgtatct ctcttatctc tacggtattt tgctgctttg cttttatttc
     2761 cttttccttg aagtttttca agggtttcac ggatttgttt ttgtacttct tcttcggttg
     2821 gctcaacttt agctacaata gccgggcgaa cccctttatt gaaaccaggt cgaattggct
     2881 gatttggtct aattgcacca ccgccttgtt gatttccttg attttgacct ccaccaggag
     2941 tccccggttt tggcggaatt cttttgcgtt tatttttatt tgcatcagat gcggcacctt
     3001 gtgttgcaga tctgggttga attttaggct cttctttttt ctttttaggc ttatcaaact
     3061 gtgataaatc aattttttgt cctgtggtag tagtacctga taatttttga tattgagttt
     3121 caaatttttc gtcaactaca ggaatatccg tcgcaacctt tggttcagct tttttaggct
     3181 cttcaacttt tggaacaatt actttaggct caattttttt aggctcaatt tttacttttt
     3241 cttcttgaac aattggttta ggttgaatgg gttcaggttt tacaacctca gcaactgggg
     3301 ttggtttaac ttcaactatc ttttcaggtt gaggaacaac aactttttta ggctctaagt
     3361 caattttacc tacttgcaca ggtacactaa caacagcttt cgctttaatt acatcttgtt
     3421 gtctatgaag atcttcttct tgttttttga gcaattctaa ttctttttct ctttcaatac
     3481 gcaaagcttc tttttcttta cgtttttcct cacttacttc tttagatgct tctttatttc
     3541 ccttatcacc tgcaaattga ttgcaaagag ttgcatatac atcttctgag atttttgtat
     3601 ttggacttgc ttcaatagca attcctttgt cttttaaata atcgacagct ctctccaaag
     3661 aaatattcaa ctcccttaaa actttattaa ttcttatatt cttttcttca gacatataac
     3721 ttttttagtg tgttcttttt cgtttgtaat ttagccttca aactctgatt tcaatattct
     3781 cataacgtct tgaacggttt cctcttctaa atcagttctt ctaaccaaat cttcaacgct
     3841 atgttttaaa atacttctag ctgtatctaa tccgattttt gcaaattcat ctattaccca
     3901 tccgtcaatt tcatccgaga actcagttaa ttcgacatcg tcttcttctt ccgcaacgct
     3961 tccttctctg ataacatcta attcataacc ggttaatagt cccgctaatt tgatgttatg
     4021 gccgccacgt ccgatagctt ttgaaacttc ttctaatttc aagtaaactt ccgctctttt
     4081 tttctcttca tcaattttaa tagaagatac ttttgcaggg cttaaagcac gtgtaataaa
     4141 taattgaata ttactggtat aattaataac atctatattt tcattaccta attcacgaac
     4201 aattccgtga atacgagaac ctttcattcc gacacaagct ccaacaggat caattctatc
     4261 atcataagaa tcaaccgcta cttttgcttt ctctcccgga atacgaacca cttttttaat
     4321 catgattaac ccgtcaaaaa tttcagggat ttcttgttca aataattttt ccaaaaactt
     4381 ttcagaagtt ctggacataa taatttgagg tttatttccc ttcaattcaa cattttcaat
     4441 aattcctcgt acattatctc cttttcggaa aaaatcggaa ggtatttgat tttcttttgg
     4501 caatacaatt tcatttccat catcatctac caaaattaca actcttggac gaacatgatg
     4561 tacttctgca gtataaattt ctcctattaa atctttaaat tgtttataaa gattagtgct
     4621 gtcgtgttca tgtattttag aaatcaaatt ttgacgaaga gctaaaatgg ctcttctccc
     4681 taaatcaatt aattttactt cttccgaaac atcttcacca acttcaaaat caggttcaat
     4741 ttttctagct tctgataatg aaatttcaga gttttcgtct tctacttcac catcagcaac
     4801 cacgatacga tttctccaaa tctccatatc acctttatca ggattaataa tgatgtcaaa
     4861 attatcatct gaaccatatt tctttttcaa ggcatttcta aacacatctt ccaaaatggc
     4921 cataagtgtc acacgatcaa ttaatttatc gtctttaaac tctgaaaatg attcaattaa
     4981 tgctatattt tccatgctaa tctattgtaa tttaaaatgt tattgtcact attgcttctt
     5041 taatttcttt ataaggtaat tctaggtttt tttcaactgt ttctttccct ttaccaattt
     5101 ttttaggttc tctagctgac catgtcaaag tgataaaatc atcattaaca gccactaatt
     5161 ttgcttcgat agtatccttt gccgttttaa tttttaaaac tcttccgata ttttttttat
     5221 attgtcttac catcttcagg ggagttgaaa cacctgcaga ggcaacttct aatgaaaaat
     5281 catgctcttc tctgtcaaga ttattttcaa ttgctctact gatgtctata caatcctgaa
     5341 gtgtcactcc atcgtcaccg tctaacgtta ctattatttt ttgactatca gtaatggtca
     5401 aatcaattaa aaagacagca ggtttctccg caagtccatc gttcaataaa gcaactactt
     5461 tttctttaaa tgtcatattt ttataaaaag aggggagctc tgctcccctc attattcaca
     5521 ttttaatcaa ataacggtgc aaatatagtg atttttttta tatttgaaaa tacattgaaa
     5581 aatgataaaa atatttgtac ctttatattt taaaaaacca acgaaaatta aaactatttt
     5641 acacgacttt ttttatgaaa aaaattttaa ttccaaccga tttttctgaa cacgcggaat
     5701 atgctttaaa agtagctgct caaattgcca aaaaacatga tggtgaaatt attttgctac
     5761 acatgctaga gttacctaac caaaccggcg atgctgtttc tagcggacac gcgttgcctg
     5821 agattatgtt ttttaaaaat gctgccttag ccaaattaga aagtttaatg gattctgatt
     5881 tccttgaagg tttaaaagta tctgaaattg ttcaatttga attagctttt gatggtatcc
     5941 ttaaattaat caagaaaaat gatattgatt taattgtaat gggttctcac ggtgcaagtg
     6001 gatttaaaga tatgtttata ggctctaatg cggagaaagt tgttcgccat tcagaagttc
     6061 cagtactgat tatcaaaaaa gaaattcctg atttcactat caacaaattt gtttttgctt
     6121 ctgatttttc agatgaagtg aaagtccctt ttgccaaagt tgttgaagtt gctaataaat
     6181 ttgaagctga attaaactta gttatgatta acacacctaa tagtttcaaa tctactttag
     6241 tggctcaaga aatcatggat aagtttgtta gtcaatttaa tatcaaaaaa gtctctacgc
     6301 acatttttaa cgatgctaat gtagaaaaag gaattttaaa tttttctaac tatatcaatg
     6361 cagatttaat tggaatgtgt acgcatggaa gaaaaggatt gtctcacttt tttaacggta
     6421 gcatcagtga agatttggta aatcatgctg ttaaacccgt tttaacattt aaaatataat
     6481 ttaaattata tttcttaaaa
//
LOCUS       DAOTCW010000126         6453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_8789_length_6453_cov_39.377149, whole genome shotgun sequence.
ACCESSION   DAOTCW010000126 DAOTCW010000000
VERSION     DAOTCW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8789_length_6453_cov_39.377149"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2267)
                     /locus_tag="P5335_09430"
     CDS             complement(<1..>2267)
                     /locus_tag="P5335_09430"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRZ75140.1"
                     /translation="IKSLVGEPMVLLLLATSLIYFLSGQVGDAIFLAVAIVLVATISL
                     YQNSRSKNALAKLKEITQPKCKVIRNGNTIEIANEQLVIGDLIVLEEGSSINADAEII
                     QSNDFSVNESILTGESLAVFKGITQEDYFVFNGTTVASGMALAKVTAIGNKTKLGEIG
                     NSLESIVEEKTPLEIQITRFVKQMVLVGAIIFLIVWGINYYHTANVLDSLLKALTLAM
                     SILPEEIPVAFTTFMALGAWRLMKIGIIVKQMKTVETLGSATVICTDKTGTITENKMS
                     LVQLYNFSEKKMVTAENFTETNSKKVIETAMWASEPIPFDAMELAIHESYQNTHHEDN
                     RPNYKMIFEYPLDGKPPMMTHILKHLNGNRIIAAKGAPEAILAVCKLSDIEKSAIEKA
                     VFSMASQGYRVLGVANSDFSGDDFPKKQQEISFHFIGLVAFYDPPKKNIQQVFEKFYK
                     AGVAVKIITGDNAITTQAIATQIGFKGADKSITGDELMQLSDKELQKRVMEINIFTRM
                     FPEAKLKIINALKNDQQIVAMTGDGVNDGPALKAAHIGIAMGKKGTEIAKQAASLILV
                     DDDLSKMLDAIAMGRKIYTNLKKAIQYIISIHIPIILTVFIPLALGWIYPSIFTPLHI
                     ILLELIMGPTCSIIFENEPMERNTMIQKPRQLSTTFFNVNELIISIIQGLVITLGTLS
                     TYQLALYFGYSETITRSMVFTALISANIFLTLVNRSFYYSVLTTLKYKNNLVLWIIGL
                     TITLTALLIYIKPFA"
     assembly_gap    2268..2367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2368..3251)
                     /locus_tag="P5335_09435"
     CDS             complement(<2368..3251)
                     /locus_tag="P5335_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRZ75141.1"
                     /translation="MKKILSSIFILALLILSCSSNKNTGNDYNPTDYMNSIKAENLSK
                     HLYIIASDEMEGRNTGEPGQKKAGEYLINHYKSIGVNFPKGATEYYQRIPSEFFKKQF
                     SPKLGDSENIWAFIEGSEKPEEILVISAHYDHVGMKNGEVYNGADDDGSGTVTLLELA
                     AAFQKAKNEGKGPKRSILFLHVTGEEHGLHGSRFYTENPLFPLNNTIADINIDMIGRR
                     GYGKETNDNYIYVIGSDRLSSDLHKLSEEANTKYIGMELDYKYNDLNDPNRFYYRSDH
                     YNFAKKGIPAIFYFNGVH"
     gene            complement(3290..4048)
                     /locus_tag="P5335_09440"
     CDS             complement(3290..4048)
                     /locus_tag="P5335_09440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRZ75142.1"
                     /translation="MKKLFVLAVLFPIISFAQLTSKTYKDGEQVLKGFAGVPKKPLSN
                     KPGILILPAWMGIDEHSKEVAQNLNNQGYYSFIADIYGEGNYPTTTKEAGQLAGYYKN
                     NVTDYQRRIQLALDQLIASGANKDKIVVIGYCFGGTGALETARGKLNVVGVVSFHGGL
                     GKAENRITDEIKPKVLVLHGADDPFVPEKEVLGFQKEMQEAKADWQMIFYSGAVHSFS
                     HKNAGNDNSKGAAYNELADKRSFTHFLTFLKEIL"
     gene            4143..4598
                     /locus_tag="P5335_09445"
     CDS             4143..4598
                     /locus_tag="P5335_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRZ75143.1"
                     /translation="MQSAAKNPEQYFQELPEERKDAMLKLRQVIKKNLPKGFEEMMGY
                     GMLGYAVPHSIYPKGYHCDPKQPLPFFGMASQKNSINIYHMGIYADKELYDWFVSEYT
                     KYSKARLDMGKSCIRFKKPEHIPFELIGELLTKISVLEWISIYESKFRS"
     gene            4743..6005
                     /locus_tag="P5335_09450"
     CDS             4743..6005
                     /locus_tag="P5335_09450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRZ75144.1"
                     /translation="MASHHTVLIIGGGTAGIMTAAQLLKKDSSLDIAIIEPSDKHYYQ
                     PAFTLVGAGTYDFEKTIRFTKDLIPKRATWIQDFATGFNPESKTISTKLNGTISYDIL
                     VVATGLVNDLDGIEGLREGLEKGVVCSNYVDSRKTWELLKAFKGGNAVFTQPTTPIKC
                     GGAPQKAAYMAADYFRKKGLSDKTNIVFATPGSVIFGVKTIADALMQVIGNYNIHLKT
                     FYAPIKIDVANKVVYFKEVSAGENKCVVNENNILKEKHTGNEIMEIPFDFLHLAPPQS
                     SPKFVKESVLVNEAGWLDVDLNSLQHKKYATIFGLGDVAGLPTAKTGAAVRKQVPVVV
                     DNILKVLKNKTADNKDYLGYSSCPLVVGYGKMILAEFNYKSEFTPDPKLKRMLVFSSN
                     KPHWRLWMLKKYMLPHLYWKKMMKGENV"
     gene            6115..>6453
                     /locus_tag="P5335_09455"
     CDS             6115..>6453
                     /locus_tag="P5335_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRZ75145.1"
                     /translation="MKDLLKQTYGFIFEEALIEEIAEVSLLRDFNEGDILIDFGDYIK
                     KMPLLIEGAIKILREDFDEGELLLYFIEKGDTCAMTMACCMGETKSEIRAVAETKGIV
                     IMIPVHKMEEW"
BASE COUNT         2092 a         1006 c         1078 g         2177 t
ORIGIN      
        1 agcaaatggt ttgatgtata ttaaaagggc agttaatgta attgttaatc caataatcca
       61 aagaactaag ttgtttttat atttcaatgt ggttaatacg gaataataaa aagatcggtt
      121 aacaagcgtt aaaaagatat tagcacttat taatgcagta aaaaccatac ttcgtgtaat
      181 agtttcgcta tacccaaaat ataaagccaa ttgataagtg gataatgttc ccaaagtgat
      241 tactaatcct tgtatgatac taattattaa ctcatttaca ttaaaaaaag tagttgaaag
      301 ctgtcttggt ttttgtatca tggtgtttct ctccatgggt tcattttcaa aaatgatgga
      361 gcaggttggt cccattatta attcaagtaa aataatatga agcggtgtga aaatacttgg
      421 ataaatccaa cctaaagcta acggaatgaa tactgttaag ataatcggta tgtgaataga
      481 aatgatatat tgaatagctt tttttaaatt ggtgtaaatt tttcgtccca tcgctatggc
      541 gtctagcatt ttagataaat catcatcaac taaaatcaat gaagcggctt gtttagcaat
      601 ttctgttcct tttttaccca tagcgatgcc aatatgtgct gcttttaatg caggaccatc
      661 atttacacca tctcctgtca tagcaacaat ttgttggtca ttttttaagg cattaataat
      721 ttttaatttt gcttccggaa acattcgtgt aaaaatgttt atttccatga ctcttttttg
      781 aagttcttta tcagaaagtt gcattagctc atcgccggtt atgcttttat cagctccttt
      841 aaaaccaatt tgtgtggcaa tagcttgagt ggttatagca ttgtcccctg tgattatttt
      901 tacggcaaca cctgctttat aaaatttttc aaacacttgc tgaatatttt tttttggcgg
      961 atcataaaac gctactaaac cgataaaatg aaaagaaatt tcctgttgtt ttttaggaaa
     1021 atcatcacca ctaaaatcag aatttgccac acctaaaaca cgatatcctt gagaagccat
     1081 cgaaaaaaca gctttttcaa tggctgattt ttctatatca gaaagtttac agactgctaa
     1141 gattgcctct ggagcacctt ttgcagcaat aatgcgattg ccatttaaat gtttaagaat
     1201 gtgagtcatc ataggaggct ttccatccaa aggatactca aatatcattt tatagtttgg
     1261 ccgattatct tcatggtgtg tgttttgata actttcatga atagctaatt ccattgcatc
     1321 aaacggaatg ggttcacttg cccacatggc tgtttcaata actttttttg aattagtttc
     1381 tgtaaaattt tcagcggtaa ccatcttttt ctcggagaaa ttataaagtt gcaccaaact
     1441 cattttattc tctgtgatag ttccggtttt atctgtacaa ataacagtgg cacttcctaa
     1501 ggtttcaacg gttttcattt gttttacaat aatacctatt ttcattaacc gccaagctcc
     1561 taacgccatg aaagtcgtaa aagctaccgg aatttcttcc ggtaaaatac tcatggctaa
     1621 tgtcaatgct tttaataaac tgtctaaaac atttgccgta tgatagtaat tgattcccca
     1681 aacgattaga aaaataatag caccaaccaa aaccatttgt ttgacaaatc gtgtaatttg
     1741 aatttctaag ggtgtttttt cttctacaat ggattccaag ctattgccaa tttctcctaa
     1801 tttagttttg tttccaatag ctgtcacttt agctagagcc attccgctgg caactgtggt
     1861 tccgttaaat acaaaataat cttcttgagt tatgccttta aaaacggcaa gcgattcgcc
     1921 ggttaaaata gattcgttga ctgaaaaatc attggattgg atgatttctg catcggcatt
     1981 gatggaagaa ccttcttcta aaacgataag gtcgccgata actaattgtt cattggcaat
     2041 ttctatcgta tttccattac gaataacttt gcatttgggt tgtgtaattt ctttaagttt
     2101 tgccagggca tttttgcttc tggagttttg gtacaacgaa atggtcgcaa ctaaaacaat
     2161 cgcaacagcc aaaaaaatag catcgccaac ctgtccgctt aaaaagtaaa taagcgaagt
     2221 agccaataac aataaaacca ttggttcacc taccaaactt tttattgnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnntca tgaacaccat taaaataaaa aatagctgga
     2401 attccttttt ttgcaaaatt gtaatgatcg gaacgataat aaaaacgatt aggatcgttc
     2461 aaatcattgt atttataatc caattccatt ccaatgtatt tagtgttagc ttcttcggat
     2521 aatttatgta aatcggaact tagacgatcc gaaccaatta cataaatata attatcgtta
     2581 gtttccttgc cataccctct tcgaccaatc atatcaatat tgatatcggc aattgtattg
     2641 tttaatggaa aaagcggatt ttcagtgtag aaacgagaac cgtgtaaacc gtgttcttct
     2701 cctgtaacat gtaaaaatag gatagatcgt tttggacctt ttccttcatt ttttgctttt
     2761 tggaaagcag ctgctaattc taataaagtg actgttccgg agccatcatc atctgcacca
     2821 ttatacactt caccattttt catcccaaca tggtcgtaat gagcagaaat aactaaaatt
     2881 tcttccggtt tttcagagcc ttctataaat gcccaaatgt tttcagaatc tcctaactta
     2941 ggactgaatt gctttttgaa aaattcagaa ggaatacgtt gataatattc tgtagcacct
     3001 tttggaaagt taactccaat tgatttgtaa tgattgataa ggtattcacc ggcttttttc
     3061 tgtccaggtt ctccggtgtt tctgccttcc atttcatcag aagcaataat atagagatgt
     3121 ttacttaaat tttcagcttt gatggaattc atataatcgg tcggattata atcattacct
     3181 gtgtttttat tagatgagca acttaaaatt agtaaggcta atataaaaat gctagacagt
     3241 atttttttca ttttcaatta tttgagcgac aaatataaca aatgtgtatt tataaaattt
     3301 cttttaggaa agttaaaaaa tgagtaaatg aacgtttatc agccaattca ttataagccg
     3361 ctccttttga attatcattt cctgcatttt tatgtgaaaa ggaatgtacc gcaccggaat
     3421 agaaaatcat ttgccaatct gcttttgctt cttgcatttc tttttgaaaa ccaagtactt
     3481 ctttttcagg aacaaaaggg tcatcagctc catgtaaaac caaaactttg ggtttaattt
     3541 catcagttat acgattttca gctttgccta aacctccatg aaaagataca actccaacta
     3601 cattcaattt tccgcgagca gtctctaacg cacctgttcc accaaaacag taaccaatca
     3661 ctacaatttt atctttgttg gctccagagg caattagctg gtctaatgct aattgaatac
     3721 ggcgttgata atcggttaca ttgtttttgt aataaccggc tagttgacct gcttctttgg
     3781 tagtagtagg ataatttcct tcaccataaa tatcggcaat aaatgaatag tatccttggt
     3841 tgtttaagtt ttgagccact tctttagaat gctcatcaat tcccatccaa gcgggtaaaa
     3901 tcaaaatacc gggtttatta cttaagggtt ttttaggaac tccggcaaaa ccttttaaaa
     3961 cttgctcacc atctttatag gttttagaag taagttgagc aaaagaaatt attggaaata
     4021 acactgcaag cacgaatagc tttttcataa ttttattttt aattttattc aaatttcgag
     4081 ataaaaagaa ttactattct atttttcgat agatttttct aattttataa aaaattaaat
     4141 cgatgcaatc agcagcaaaa aaccccgaac aatattttca agagctacct gaagaacgta
     4201 aagatgcaat gttgaagtta cgacaagtta taaaaaagaa tctccccaaa ggttttgaag
     4261 agatgatggg gtatggaatg ttgggttatg ctgttccaca ttccatctat ccaaagggat
     4321 atcattgtga cccaaaacag ccattgcctt tttttggaat ggcgtctcaa aaaaattcca
     4381 tcaatattta tcatatggga atatatgcag acaaagagtt gtatgattgg tttgtgtcag
     4441 agtatactaa atatagcaaa gcacgattag acatgggaaa aagttgtatt cggtttaaaa
     4501 aacctgaaca tattcccttt gaactaatag gtgaattatt gaccaaaatt tccgtcttag
     4561 agtggatttc gatatatgaa agtaaattta gatcataatt atttattttt catttattct
     4621 gtctttttat tatactattt ctattttttt actgtattta tttgtaaaat ctgatttttg
     4681 taacttttgt tacatttaaa taaaatgcac tccattattt ttactgttct aaaaaaataa
     4741 aaatggcttc acatcacaca gttttaataa ttggaggagg tactgcgggt attatgacag
     4801 cggcacaact tttaaaaaag gattcttcat tagatatagc aattatagaa ccttcggata
     4861 aacattatta tcaaccggct ttcactttgg taggagcggg tacgtatgat tttgaaaaaa
     4921 caattcgttt tacaaaagat ttaattccaa aaagagcaac ttggattcaa gattttgcta
     4981 ctggatttaa tcctgagtca aaaaccattt cgacaaaatt gaatggaaca atatcatatg
     5041 atattttagt agttgcaacc ggactcgtta atgatttgga tggaatagaa ggtttaagag
     5101 aaggattaga aaaaggtgtt gtttgtagta attatgttga ttccagaaaa acatgggagc
     5161 ttttgaaagc ctttaaaggt ggaaatgccg tgtttactca acctacaact ccaattaaat
     5221 gtggaggagc tccacaaaaa gcagcttata tggctgcaga ttattttaga aaaaaaggac
     5281 tttcggataa aacaaatatt gtttttgcca ctccgggttc tgttattttt ggagtaaaaa
     5341 ctatcgcaga tgccttaatg caggttatag gtaattataa tattcatctt aaaacatttt
     5401 acgccccaat taaaattgat gttgcaaata aagttgtcta ttttaaggaa gttagtgcgg
     5461 gggaaaataa gtgtgttgtc aatgaaaata atatattgaa agagaaacac accggaaatg
     5521 aaataatgga aattcctttt gattttttgc atttagctcc accacaatct tctcctaaat
     5581 ttgtaaaaga atcggtttta gttaacgaag caggttggtt agatgtcgat ttaaatagtt
     5641 tgcagcataa aaaatatgct actattttcg gattagggga cgtagcggga ttaccaactg
     5701 caaaaacagg agcagctgtt agaaaacagg ttccggttgt agtagataat attttgaagg
     5761 tgttaaaaaa taaaacagct gataataaag attatttagg atattcttct tgtcctttag
     5821 tagtaggata cggaaaaatg attttagcag agtttaatta taaaagcgaa tttacgccgg
     5881 acccaaaatt gaaaagaatg ttagtttttt ccagcaacaa accgcattgg cgattatgga
     5941 tgcttaaaaa gtatatgctg cctcatttat attggaaaaa aatgatgaaa ggtgaaaatg
     6001 tttaaaacta aattaatcct aaattctaaa ggcaaaacaa ccacagtttt gtcttttttt
     6061 atgtttatgt gatttatttt tctcaatttt acagttacaa aattttgtat gctaatgaag
     6121 gacttactaa aacaaactta tggttttatt tttgaagagg cgttaattga agaaattgca
     6181 gaagtttctt tattgcggga ttttaatgaa ggagatatat taattgactt tggtgattat
     6241 atcaaaaaaa tgcctttact aattgaagga gccataaaaa tattgcggga agattttgat
     6301 gaaggagaat tgttactcta ttttattgaa aaaggagata cttgtgcaat gactatggct
     6361 tgttgtatgg gagaaactaa aagtgaaata cgtgctgtag ctgaaacaaa aggtatagta
     6421 attatgattc cggttcacaa aatggaagag tgg
//
LOCUS       DAOTCW010000127         6450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_8794_length_6450_cov_36.867553, whole genome shotgun sequence.
ACCESSION   DAOTCW010000127 DAOTCW010000000
VERSION     DAOTCW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8794_length_6450_cov_36.867553"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="P5335_09460"
     CDS             <1..552
                     /locus_tag="P5335_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRZ75146.1"
                     /translation="GQGRRKGKGEWIQPGKKLVVDEKPKTYEEIVLEYALGTKAVLGK
                     NLMGAIQSKGTMHAMEFCNVQAMPLIDSMSTHFNAKIKRVSDKNRNPNNKANAEELQY
                     IEKFKTDLVNKRESKPVVIEKGNMVQFYYPIPTNTMCLQCHGTQIKPEVSKQILKLYP
                     NDLAVGYSENEIRGIWSITFEKN"
     gene            555..1151
                     /locus_tag="P5335_09465"
     CDS             555..1151
                     /locus_tag="P5335_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRZ75147.1"
                     /translation="MIDFWNIRYAENDFAYGKEPNQFLVENIDHIPKGKMLFPAEGEG
                     RNAVYAAQKGYEVYAFDTSIKAKEKAIELAKAHNASINYQVGMLSDLHYPENSFDGIV
                     LIYAHVPNEIRNDFHKHLIRLLKPGGVVLFEAFSKEQLNYSSGGPKDYDMLFSEEEIK
                     TEFSSLQFTQLETLEIDLNEGKYHQGRASVVRFKATKL"
     gene            1148..1600
                     /locus_tag="P5335_09470"
     CDS             1148..1600
                     /locus_tag="P5335_09470"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRZ75148.1"
                     /translation="MKINSKDIVLVSIQFVLFILYTIPFGGSLEFFSWFKTIGLITSI
                     VGFVILVLALLQLNKNLSPFPTPKNNAVLIQNGLYAWVRHPIYTGLLLLFFGYSIYQA
                     SLFKLFITFLLWVLFHFKTQYEELQLQRKFPDYTTYKKNKGRFFPKFI"
     gene            1672..2265
                     /locus_tag="P5335_09475"
     CDS             1672..2265
                     /locus_tag="P5335_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRZ75149.1"
                     /translation="MTTEEKIFEAAHKVFQQKGFSGARMQEIADEAGINKAMLHYCFK
                     NKELLFEAVFMKAFAQLAPQINEIFNSDASVFDKIRRFTDSYISFVIENPFLPPFIIQ
                     EMNNNPEFVERFLNHKNRPNPTILIAQIEKEIAEGIIKEINPKQLLLDIFSMTIFPFA
                     AQVLVKGMIQINEKEFFELMEQRKFHISEQIINAIKK"
     assembly_gap    2408..2507
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2508..3661
                     /locus_tag="P5335_09480"
     CDS             <2508..3661
                     /locus_tag="P5335_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRZ75150.1"
                     /translation="TKNYPLAKQAELLKLKNEFETEAIQKGKLPKIDWNAQATYQSDV
                     TEVPVMTIEPLNNDQYRTTIDVNQLIYNGGLIDANAKLKEAQTKTQQQQVEVALYQLK
                     FKINYYYFSILVLQERENILKSKQEQLVLKINEIKTGVKFGAILPSSEKVLEAENLKL
                     NQQLTEINSEKIKLLQNLSSLTFSPISSATILEKPEITNKLNKESLRPELLLYDLQTE
                     QIETSAAVLTKNLFPKINAFGFAGYGNPGLNMLDNSFQPFYMLGIRANWNIFDWNKTK
                     SEKEALTVSKEIVASEKETFQLNAQMQLQEFENEIAKAESILISDNEIIQLRMDVLKS
                     SDSQLKNGVITSSEYIIDFTNLYEAKINQKVHEIQLELAKANYQITKGY"
     gene            3663..4535
                     /locus_tag="P5335_09485"
     CDS             3663..4535
                     /locus_tag="P5335_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRZ75151.1"
                     /translation="MKKLVLLSLTVLFSCNGNDEKADGYGNFEATEVTISAEANGKIE
                     FLNLEEGDLLTPKTQVGLIDTMQLYFAKQQLIASKNTVYSKSANVLSQQKVLQEQLKT
                     MQIEKNRIHNMFAENAATKRQVDEIDGRVNVLQEQMKSVETQNAPIINEIESLEVQIK
                     KMNDQIQKCKIVNPIKGTVLVKYAEPNEVTSFGKPLYKIADISEMTLRVYISERQLAS
                     VKIGQKVNVKIDSSKDSKSYSGTISWIAAEAEFTPKIVQTKEERVNLVYAVKVLVKND
                     GSLKIGMPGELWLK"
     gene            4547..4918
                     /locus_tag="P5335_09490"
     CDS             4547..4918
                     /locus_tag="P5335_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75152.1"
                     /translation="MKKLVVIVTIAITVIGCKISQTNLKSNTMNLKNLHTEDKAVQTH
                     LLFQPTEAKVVSLQIKKNEQLAEHVSKVPALLVCISGKALYNDETGLKILLTNGDYVA
                     IKQDVKHWIDAQEESNFLLIK"
     gene            4925..5821
                     /locus_tag="P5335_09495"
     CDS             4925..5821
                     /locus_tag="P5335_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRZ75153.1"
                     /translation="MSIVIQNISKSYQSLQAVDAISFEVKEGELFGLIGPDGAGKTTI
                     FRMLTTLLIPSFGEAKVLGFDVVKEYKSIRNSVGYMPGKFSLYQDLTVEENLNFFATI
                     FGTTIEENYELIKDIYIQIEPFKNRKAGALSGGMKQKLALCCALIHAPKVLFLDEPTT
                     GVDPVSRKEFWQMLKRLQQKGITILVSTPYMDEASLCDRIALIQKGKILKIDAPVTII
                     SEFDKTIYAVSATNKHQLILDLKGYPSMHSVYAFGEFIHYIDKKSDFHSQDLNHYLIS
                     KNHQDIEILPIEPTIEDIFMDL"
     gene            5832..>6450
                     /locus_tag="P5335_09500"
     CDS             5832..>6450
                     /locus_tag="P5335_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRZ75154.1"
                     /translation="MNQEKIITVQNLTKIFGTFTAVKGISFEVDKGEIFGFLGANGAG
                     KTTAMKMLIGISKPSSGKAVVAGYDVVENTESVKKSIGYMSQKFSLYDDLTIKENITF
                     FGGIYGLSRKNIKLKMNDLLTELSLQDVANKLVGSLPLGWKQKLSFSIALLHEPQIVF
                     LDEPTGGVDPITRRQFWEMIYAEANKGTTIFVTTHYMDEAEYCDRV"
BASE COUNT         2399 a          939 c         1091 g         1921 t
ORIGIN      
        1 ggacaaggaa gaagaaaggg aaaaggcgaa tggattcagc ccggtaaaaa attggtagtt
       61 gatgaaaaac ctaaaaccta tgaagaaatt gtattagaat atgctttggg aactaaagct
      121 gttttaggta aaaacctaat gggagcaata cagtccaaag gaaccatgca tgccatggaa
      181 ttttgtaatg tacaagcaat gcctttaatc gacagcatgt caacgcattt taacgcaaaa
      241 ataaaacgag tttctgataa aaacagaaac ccgaataaca aagctaatgc tgaggaactc
      301 caatatatcg aaaaattcaa aaccgattta gtgaataaaa gagaatcaaa acctgtagtg
      361 attgaaaaag gaaatatggt gcaattttat tatcctattc caactaacac gatgtgtttg
      421 caatgccatg gaacacaaat caaaccggaa gtttctaaac aaatactcaa attgtatccg
      481 aatgatttgg ctgtaggtta tagtgaaaat gaaatcagag gcatttggag tattactttc
      541 gaaaagaact aaatatgata gatttctgga atattcgtta tgcagaaaat gattttgctt
      601 atggaaaaga acccaaccaa tttttagttg aaaatattga tcatattcct aaaggaaaga
      661 tgctttttcc tgcggaaggc gaaggaagaa atgcagttta tgctgcacaa aaaggatatg
      721 aagtttatgc gtttgacaca agtataaagg ctaaagaaaa agcaatagaa cttgccaaag
      781 ctcataatgc atccataaat taccaagttg gaatgttatc cgacttacat tacccggaaa
      841 atagttttga tggaatagtt ttgatttatg ctcatgttcc aaatgaaatt cgaaatgatt
      901 ttcataagca tttaatccgt ttattaaaac ccggaggtgt agtacttttt gaagcgttta
      961 gtaaagaaca attaaattat tcttccggag gaccaaaaga ctatgatatg ctgttttcag
     1021 aagaagaaat taaaactgaa ttttcgtctt tacaattcac acaattagaa acgttagaaa
     1081 ttgatttaaa cgaaggaaaa taccatcaag gtagagcaag tgtggtccga tttaaagcca
     1141 ccaaattatg aaaattaact ccaaagacat tgttttggtt agcattcaat ttgttttgtt
     1201 tattttatat acaattcctt ttggtggatc tttggagttt ttttcatggt ttaaaaccat
     1261 tggtttgatt acttcaatag ttggatttgt cattttagtt ttagcacttt tacaattgaa
     1321 taaaaatctc tctccatttc caactccaaa aaataatgct gttttaattc aaaatggatt
     1381 gtatgcttgg gttcgacatc cgatttacac gggattactt ttactttttt ttggctatag
     1441 tatctatcaa gcttctctgt ttaaattatt cataactttt ttactttggg tgttgttcca
     1501 tttcaaaaca cagtatgagg aattgcaatt gcaacgaaag tttcctgatt acactaccta
     1561 caagaagaat aaaggtcgct ttttcccaaa atttatttaa ttattttaag tcacttaatt
     1621 atttttatgt acatttgtta accaaatggt taaaccaaat agttagtaaa aatgactaca
     1681 gaagaaaaaa tatttgaagc agctcacaaa gtctttcaac aaaagggatt ttcaggagcc
     1741 cgaatgcagg aaattgcaga cgaagccggt attaacaaag cgatgttaca ttactgtttt
     1801 aaaaataagg agttactttt tgaagcagtt tttatgaaag catttgctca gttagctcct
     1861 cagataaatg aaatatttaa ttcagatgct tcggtttttg ataaaatcag aaggttcacg
     1921 gatagttata tttcatttgt tattgaaaat ccatttttac ctccttttat cattcaggaa
     1981 atgaataata atcctgagtt tgtagagcgt tttttaaatc ataaaaacag acccaatcca
     2041 acaattctga ttgctcaaat agaaaaagaa attgcggaag gaataattaa agaaataaat
     2101 ccaaagcaac ttttactaga tattttttca atgacaattt ttccttttgc tgcacaagtt
     2161 ctagtaaaag gaatgattca aataaatgaa aaagaattct ttgaattaat ggaacagcga
     2221 aaatttcata tttccgaaca aattattaat gctataaaaa aatgaaaaaa ataatttctt
     2281 tgatattagt attgatgacc ttcgtatcaa atgctcaaga aaaacttaat ttggatgcct
     2341 gttatcaatt agtaactaaa aattatccgt tggccaaaca agcagaatta ttgaaactaa
     2401 aaaatgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntaa ctaaaaatta
     2521 tccgttggcc aaacaagcag aattattgaa actaaaaaat gagtttgaaa cagaagccat
     2581 tcaaaaggga aaacttccaa aaatagattg gaatgcacaa gccacttatc aatcagatgt
     2641 gacagaagta cctgtcatga caatcgaacc gcttaataat gaccaatatc gaacaacaat
     2701 tgacgtcaat caattgattt ataatggtgg cttaattgat gcaaatgcta aattaaaaga
     2761 ggcacaaaca aaaacacaac aacaacaagt tgaagttgct ctttatcagt tgaaatttaa
     2821 aatcaattac tattattttt caattttagt gcttcaggaa cgcgaaaata ttttaaaatc
     2881 aaaacaagaa caactagttt taaaaataaa tgaaattaaa accggtgtaa agtttggagc
     2941 tattcttccg tcgtcagaaa aagtattaga agccgaaaac ttgaaattaa atcaacaatt
     3001 aacagaaata aattcagaaa aaatcaagtt acttcaaaat ttgtcctctt taactttttc
     3061 acctataagc agtgcaacca ttttagaaaa acctgagatt acgaataaat tgaataaaga
     3121 aagtttacga cctgaattgt tgctatatga tttacaaacc gaacaaattg aaacatcagc
     3181 tgctgttttg actaaaaatc tgtttcctaa aatcaatgct tttgggtttg caggttatgg
     3241 aaatccgggt ttgaatatgc ttgataattc atttcagcct ttttatatgt tgggaattcg
     3301 tgccaattgg aatatttttg attggaataa aacgaagtct gaaaaagaag cactaacggt
     3361 ttcaaaagaa atagtagctt ctgaaaagga aacctttcaa ttgaatgcac aaatgcaatt
     3421 gcaagaattt gaaaatgaaa ttgcaaaagc agagtctatt ttgatatcgg ataatgaaat
     3481 tattcaattg cgaatggatg tattgaaatc gtctgattca caactcaaaa atggagtgat
     3541 tacttcgtct gaatatatta ttgattttac caatttatat gaagcaaaaa tcaatcaaaa
     3601 agtacatgaa attcaattag aattggcaaa agcgaattat caaatcacca aaggatatta
     3661 atatgaaaaa attagtctta ctctcactaa cggttttgtt ttcttgtaat ggaaacgatg
     3721 aaaaagccga tggctatgga aattttgaag caaccgaagt aaccatttct gcggaagcaa
     3781 atggaaaaat tgaatttcta aaccttgaag aaggtgattt attgaccccc aaaacacaag
     3841 taggcttaat agatacgatg caattgtatt ttgcaaaaca gcaattgata gcctcgaaaa
     3901 atacagttta ttcaaaatct gccaatgttt tatcacagca aaaggtgttg caagaacagt
     3961 tgaaaacgat gcaaattgaa aaaaatcgaa ttcataatat gtttgctgaa aatgctgcca
     4021 ccaaacgtca agtagatgaa attgacggaa gagtaaatgt acttcaagaa caaatgaaaa
     4081 gtgtagaaac tcaaaatgca ccaatcatta atgaaataga atcattagaa gttcaaatta
     4141 aaaaaatgaa tgatcaaatt cagaaatgta aaattgtaaa tccaatcaaa ggcactgttt
     4201 tagtaaaata tgccgaaccg aatgaagtaa cttcttttgg aaaaccgctt tataaaattg
     4261 ctgatatttc tgaaatgacg ttgagggttt atatcagcga aagacaatta gcttctgtaa
     4321 aaataggaca gaaagtaaat gtaaaaattg acagttcaaa agattcaaaa tcctattccg
     4381 gtactatttc atggattgct gctgaagctg aatttacgcc aaaaattgtt caaaccaaag
     4441 aagaacgcgt gaatttagtg tatgccgtga aagtacttgt aaaaaacgac ggaagtttaa
     4501 aaattggtat gcctggtgaa ctatggctca agtaaatctg taaatcatga aaaaattagt
     4561 agtaatcgta actattgcaa tcacagtaat tggttgcaaa atttcccaaa caaatttaaa
     4621 atcaaatact atgaacttaa aaaatttaca tacagaagac aaggctgttc agacccatct
     4681 attatttcaa ccaacagagg cgaaagtagt atcgttacaa atcaaaaaaa acgaacaatt
     4741 ggcagaacat gtttcaaaag taccggcttt attggtttgc atctcgggta aagctctata
     4801 caatgatgaa acaggtttaa aaattctttt aaccaatggc gattatgttg ccattaaaca
     4861 agacgtcaaa cattggattg atgctcaaga ggaaagtaac tttttactaa taaaatagcg
     4921 attaatgagt atagtaattc aaaatatttc caagtcctat caatcattac aagcggttga
     4981 tgctatttct tttgaagtaa aagaagggga actttttgga ttaattggtc ccgatggtgc
     5041 aggtaaaaca acaattttca gaatgttaac cacactttta attccatcat ttggtgaagc
     5101 taaagtgctt ggctttgatg tagtaaaaga gtataaatcc atcagaaatt cggtaggtta
     5161 tatgcccggg aaattttcat tatatcagga tttgactgtt gaagaaaatt tgaatttttt
     5221 tgccactatt ttcggaacaa caattgagga gaattatgaa ctcatcaaag atatctacat
     5281 tcaaattgaa ccttttaaaa acagaaaagc cggagcatta tcaggaggta tgaaacaaaa
     5341 attagcgttg tgttgtgcgt taattcacgc tccaaaagta ctttttttag acgaacctac
     5401 cactggagtt gatccggttt cgcgtaaaga gttttggcaa atgctcaaac gcttacaaca
     5461 aaaagggatt actattttag tttcaactcc ctacatggat gaagcttcgc tttgtgaccg
     5521 aatagcctta attcaaaagg gtaaaatttt aaaaattgac gctccggtta cgattatttc
     5581 agaatttgac aaaactattt atgcggtgag tgcgacaaat aaacaccaac taattttaga
     5641 tttaaaagga tatccatcga tgcacagtgt gtatgctttt ggagaattca ttcattacat
     5701 cgataaaaaa agtgactttc attcacaaga tttgaaccac tatttgattt cgaagaatca
     5761 tcaggatata gaaattttac caatagaacc taccatcgaa gacattttca tggatttata
     5821 atgagagaaa aatgaaccaa gaaaaaataa ttacggtaca aaatctaacc aaaatttttg
     5881 gaacatttac agcggtgaaa ggcatttcat ttgaggttga taaaggggaa atatttggtt
     5941 ttctaggagc caatggtgca ggaaaaacaa cagctatgaa aatgcttatc ggtatttcaa
     6001 aaccttcgtc aggaaaagct gttgtggcag gttatgatgt agttgaaaac acagaatcgg
     6061 tcaagaaaag cattggctat atgagtcaga aattttcatt gtatgacgat ttaactatca
     6121 aagaaaatat tacttttttt ggtggaattt acggcttatc aagaaagaac atcaaattga
     6181 aaatgaatga tttgctcact gagttgtcac tacaagatgt tgcaaataaa ttggtaggct
     6241 cattaccctt gggatggaaa caaaaattat ctttttcaat tgccttgctc cacgaacctc
     6301 aaattgtatt tttagatgaa cctacaggag gagtagatcc aatcacccga cgacagtttt
     6361 gggaaatgat ttatgcagaa gcaaataaag gcaccactat ttttgtgaca acacattata
     6421 tggacgaagc ggaatattgt gaccgggttt
//
LOCUS       DAOTCW010000128         6430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_8828_length_6430_cov_54.832784, whole genome shotgun sequence.
ACCESSION   DAOTCW010000128 DAOTCW010000000
VERSION     DAOTCW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8828_length_6430_cov_54.832784"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /gene="hemA"
                     /locus_tag="P5335_09505"
     CDS             <1..1152
                     /gene="hemA"
                     /locus_tag="P5335_09505"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRZ75155.1"
                     /translation="ANVLFQAKQEGLDSLFIISTCNRTEIYGFAEHPFQLIKLLCDNS
                     LGTVEEFQKVAYVFKSQEAINFLFRVGTGLDSQILGDFEIISQIRNGFVESKKIGLVN
                     SFLERLVNAVIQASKRVKNETELCSGATSVAFASVHYIFNTVSDLQNKNILLFGTGKI
                     GRNTCENLVKHTKNENITLINRTKTKAEKIAGKFNLIVKDYVDLQVEIQRADVLVVAT
                     GAQNPTIDKSLLNLKKPLLILDLSIPKNVDENVKQLEGVTLIHMDHLSQLTDETIEKR
                     KEQIPFAEAIIAEIREEFVSWMKSRKFAPTIHALKEKLNTIKNNELSFQRKKITNFDE
                     EQAEIISNRIIQKITTHFANHLKDDNTTVDESIEWIERVFQIETIPYNE"
     gene            1142..2068
                     /gene="hemC"
                     /locus_tag="P5335_09510"
     CDS             1142..2068
                     /gene="hemC"
                     /locus_tag="P5335_09510"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRZ75156.1"
                     /translation="MMSKIIKIGTRDSELALWQAHTVQQKLHDLGFQTEIVAVKSTGD
                     IILDKPLYELGITGIFTKTLDIAMLKGEIDIAVHSMKDVPTALPKGIVQAAVLERAST
                     LDILVHKGDLSFLDNMGIIATGSLRRKAQWLNKFPLHQVEDLRGNVNTRLQKLKENPW
                     NGAIFAAAGLERINLKPDSFINLDWMIPAPAQGAMVVVAMEQDDYTKEALAQLNHHET
                     EICTHVERQFLKTLEGGCTAPIGALAVIQNDKLLLKGILFSLDGSKKLDIEKQINIDL
                     YQNFGELCATEILKNGGSELMQSIKKALDKKH"
     assembly_gap    2072..2171
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2172..3107
                     /locus_tag="P5335_09515"
     CDS             <2172..3107
                     /locus_tag="P5335_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRZ75157.1"
                     /translation="ENPEIENKLFFSVFHSISAFCNAGFSTMTNGLSENYIRFNYYMQ
                     WVVIFLVVFGGLGYNIIFNFYQYLKIHVVEFFDRKRIHKQISIMTLNSKIVLYTTGAL
                     LVGGFIFLWLSEMNNMLNLHDTFFGKITATAFNSVTPRTAGFNTMDFTQYTVPSLLFI
                     IFLMWIGASPASTGGGIKTSTFALATLNIFAIARGKSRIQLFGRRISSESTSRAFAIL
                     CISLIIIGFAILALLIFEPKGTDLLTVVFECFSAYSTVGLSLNFTPTMTEPSKYVIIA
                     TMFIGRIGMLNLMIGLLRQINHQFYEYPKENILIN"
     gene            3119..3808
                     /locus_tag="P5335_09520"
     CDS             3119..3808
                     /locus_tag="P5335_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRZ75158.1"
                     /translation="MKIIIFGLGNFGMSLALSLTETGNEVIGVDKQMDKVNLIKDKIS
                     HAVCMDSTNELAYESLPLKDADKVVVGIGENEGAAIITTAIIKKLSNVKIISRALSPI
                     HDTVLEAMGIYSIIHPEQESADRLTKQINFKTTLENYQLDNNYTISEVRSKPEFHGKT
                     LHELNSIDKYHLNVVTIIRKKEKTNLIGKKSIVKETIGLPTPETVIFEDDILVVFGEN
                     KNISIYCDGQE"
     gene            3811..4491
                     /locus_tag="P5335_09525"
     CDS             3811..4491
                     /locus_tag="P5335_09525"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRZ75159.1"
                     /translation="MKNKIRILSTKKLLPNQKQFLLNANFCLVEADFIKIDFQKNKIE
                     SKYDYLIFTSQNAVNSVLSNESFEDLKEKKCFCVGKKTKVLLEQNGFQVLQAFDYVEE
                     LTPFLITNYSHSTFTFFSGNLRQDTLPISFQKAAIIFEEKQVYNTSLTPKKINSDVDG
                     ILFFSPSAVRSYLIENTFSNEICFCIGTTTAKEVEKTAKNIVIANQTTVENVIIQSIN
                     YFKNENSK"
     gene            4537..5562
                     /gene="hemE"
                     /locus_tag="P5335_09530"
     CDS             4537..5562
                     /gene="hemE"
                     /locus_tag="P5335_09530"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRZ75160.1"
                     /translation="MIKNDLFLRALNNETVERPPVWMMRQAGRYLPEFRALRDKYDFF
                     TRCETPELAAEITVQPIRIVKPDAAILFSDILVVPRAMGIHVELKDNLGPIIPNPIRT
                     IEQVDQVYVPNVNETLGYVFDAIKLTKEMLNDEVPLIGFAGSPWTIFCYAVEGKGSKS
                     FDTAKGFCFSNPVAAHILLQKITDTTILYLKEKVKAGCNAIQIFDSWGGMLSPVDYQE
                     FSWRYINQIVEALAPHTKVIVFGKGCWFALNDMAKSKASALGVDWTCSPRNARYLSGG
                     RITLQGNFDPSRLLSPIPTIKKMVHEMIDEFGKDNYIVNLGHGILPNIPVDHAKAFVE
                     AVKEYKK"
     gene            5567..6103
                     /locus_tag="P5335_09535"
     CDS             5567..6103
                     /locus_tag="P5335_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRZ75161.1"
                     /translation="MIKTDLFFIDYLVLEDAASLSEMMLCNHDNFKRYFPITLSKNKS
                     IEDSLKFIKEKNIECHAKSEFTFVIKDKQLSKAVGIIIIKEINWEIKQGEFAYCMSSE
                     FENKGWMSNAIALTSKYAFEVLGFNKLQIIAHRTNFGSCKVAEKCGFQWQKTLINEYT
                     PPNESPLDMELYELYNER"
     gene            6093..>6430
                     /locus_tag="P5335_09540"
     CDS             6093..>6430
                     /locus_tag="P5335_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HRZ75162.1"
                     /translation="MRDKFYQYILQLQNQITSKLEAIDGVAKFREDIWERPEGGGGKT
                     RVIENGLVFEKGGVNISAVHGKLPDSMQKLFNVGDADFFACGLSLVIHPKSPMVPTVH
                     ANWRYFEMYD"
BASE COUNT         2272 a          953 c         1154 g         1951 t
ORIGIN      
        1 gcaaacgttt tatttcaagc aaaacaagaa ggattagaca gtttatttat tatctcaact
       61 tgtaaccgta cagagatata tggttttgca gaacaccctt ttcagttaat caaattactt
      121 tgtgataata gtttaggaac tgttgaagaa tttcaaaaag tagcctatgt attcaaaagc
      181 caagaagcta ttaatttttt atttcgtgta ggaactggtt tagatagtca aatattaggt
      241 gattttgaaa ttatcagcca aatcagaaat ggatttgttg aaagtaaaaa aatcggattg
      301 gtaaattctt tcctagaaag attagtgaat gcagttatac aggcaagtaa acgtgtaaaa
      361 aacgaaacgg aattatgcag cggagccact tcggttgctt ttgcttcggt tcattatatt
      421 tttaataccg tttctgatct tcaaaataaa aacattttgc ttttcggtac tggaaaaata
      481 ggccgaaaca cctgcgaaaa tttggtaaag cataccaaaa atgaaaacat tactttaatt
      541 aaccgaacga aaacaaaggc tgaaaaaatt gccggaaagt ttaatttaat tgtaaaagat
      601 tatgtcgatt tacaagttga aattcaacgg gcagatgttt tagtggtggc tacaggagcc
      661 caaaatccta cgatagataa atccttgttg aacctcaaaa agccattgtt aattttagat
      721 ttgtctattc ccaaaaatgt agatgaaaac gtgaaacaat tggaaggtgt tacgttgatt
      781 catatggacc atttgtcgca attgaccgat gaaaccattg aaaaaagaaa agaacaaata
      841 ccttttgctg aagcaattat tgctgaaatc agagaagaat ttgtttcttg gatgaaatct
      901 cgaaaatttg cccctaccat tcatgcttta aaagaaaaac ttaatactat taaaaataac
      961 gaattgagtt ttcaacgaaa aaaaattact aattttgatg aagagcaagc tgaaataatc
     1021 agtaaccgaa tcattcaaaa aataaccacg cattttgcga atcatttgaa agacgataac
     1081 accacagttg atgaaagcat cgagtggata gaacgtgttt ttcaaattga gaccatacca
     1141 tataatgagt aaaattatta agataggaac ccgtgacagt gagttagcac tttggcaagc
     1201 tcatacggtt caacaaaaac ttcatgattt aggctttcaa accgaaattg tagctgtaaa
     1261 atcaaccgga gatataattc tagacaaacc tttatacgaa cttggaatta cagggatttt
     1321 caccaaaaca ttagatattg ctatgttaaa aggtgaaatt gacattgctg ttcattccat
     1381 gaaagacgtg ccaacggctt tgcccaaagg aattgttcag gctgcagttt tagaaagagc
     1441 ttcaacattg gatattttgg tacacaaagg cgatttgtct tttttggata atatgggaat
     1501 cattgcaaca ggtagtttac gaagaaaagc acaatggttg aataaatttc ccttgcatca
     1561 agtagaggat ttacgtggaa acgtaaatac acgattgcaa aaattaaaag agaatccatg
     1621 gaacggagcc atttttgcag cagcaggatt ggaacgcatc aaccttaaac ccgattcatt
     1681 tattaatctc gattggatga ttcccgcacc ggctcaagga gcaatggttg tggttgctat
     1741 ggaacaggat gattatacaa aagaggcttt agctcaatta aatcatcacg aaacggagat
     1801 ttgtactcat gttgaaagac agtttttgaa aacgttggaa ggcggttgta cagcaccaat
     1861 tggagctctt gctgttatcc aaaatgataa gctattatta aaaggaattt tattttcatt
     1921 agacggttca aagaagctag acatagaaaa acaaataaat atcgatttat atcaaaattt
     1981 tggagaactt tgtgctacag aaattttaaa aaacggtggt tcagaactca tgcaatcaat
     2041 taaaaaggca ttagataaga aacactaatc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn ngagaatccg gaaattgaaa ataaattgtt tttttcggtt tttcattcta
     2221 tctcagcctt ttgtaacgct ggtttttcaa cgatgacaaa cggtttaagt gaaaattata
     2281 tccgttttaa ttactacatg caatgggtag ttattttttt agtggttttt ggtggtttag
     2341 gatacaatat catttttaat ttttatcaat atttaaaaat acatgtagta gagttttttg
     2401 atagaaaaag aattcataag caaatcagta ttatgacgct caattcaaaa attgtacttt
     2461 atacaaccgg agctttattg gttggagggt ttattttcct ttggttatct gaaatgaata
     2521 atatgcttaa tttacacgat acttttttcg gaaaaattac agcaacagca tttaattctg
     2581 taacgcccag aacggcaggt ttcaacacca tggattttac acaatatacg gttccttcgt
     2641 tattatttat tattttttta atgtggattg gtgcttcacc ggcatcaact gggggaggta
     2701 taaaaacaag cacttttgcc ttagcaacgt taaatatttt tgccattgct agggggaaat
     2761 ctaggattca attatttgga agaagaattt caagcgaatc tacttcacga gcgtttgcta
     2821 ttttatgtat ctcattgata ataataggat ttgctatttt agccttgttg atttttgaac
     2881 caaaagggac tgatttactt accgttgttt ttgaatgttt ctcggcttac agcacggttg
     2941 gactaagttt gaactttaca ccaacaatga ctgagccaag taaatatgtt attatagcca
     3001 caatgtttat aggcagaatc ggaatgctca atttaatgat aggtttatta cgtcaaataa
     3061 atcatcagtt ttatgaatat ccgaaagaaa atattttaat taactaatac ctaagaaaat
     3121 gaaaataatc atttttggac ttggaaactt cggaatgtcg ttagctttaa gcttaacaga
     3181 aaccggcaat gaagtaattg gagttgacaa acaaatggat aaagtcaatc tgattaaaga
     3241 taagatttct cacgctgttt gtatggattc tacaaatgag ttggcttatg aatctttacc
     3301 acttaaagat gccgataaag tagtagtagg aattggagag aatgaaggtg cagcaattat
     3361 tacaacagcc atcatcaaaa aattatctaa cgtaaaaatt atcagtagag cattatcacc
     3421 tattcacgat acggttttag aagcgatggg gatttacagt attattcatc cggagcagga
     3481 aagtgccgat cgattaacta aacaaattaa ttttaaaaca acgttagaaa attatcaatt
     3541 agataataat tataccattt ccgaagttag atcgaaacct gaatttcacg gaaagacttt
     3601 acacgaactc aattcaattg ataaatatca cttgaatgtg gtaaccatta ttcgtaaaaa
     3661 agaaaaaacg aatttaattg gaaaaaaatc aattgtaaaa gaaacaattg gattacctac
     3721 accggaaaca gtaatttttg aagacgatat attagttgtt tttggtgaaa acaagaacat
     3781 ttcaatttat tgtgacggtc aggaataaaa gtgaaaaaca aaatcagaat actatctacc
     3841 aaaaaacttc tgcctaacca aaagcagttc ttattaaatg caaacttttg tttagtagaa
     3901 gcagatttta tcaagattga ttttcaaaag aataaaatag aatcaaaata tgattatttg
     3961 attttcacta gccaaaatgc cgttaatagc gttttatcca atgaaagttt cgaggattta
     4021 aaagaaaaaa aatgcttttg tgtgggaaaa aaaacaaaag tacttttaga acaaaatgga
     4081 tttcaagtat tacaagcatt tgattatgta gaagaattaa ctcctttttt aataaccaat
     4141 tacagtcata gtacatttac ctttttttcc gggaatttaa gacaagatac tttacctatt
     4201 tcttttcaaa aagcagctat catttttgaa gagaaacaag tgtataatac ctctttaacg
     4261 ccaaaaaaaa tcaatagcga tgtagatgga attttgtttt ttagtccatc agctgttcgg
     4321 agctatttaa tagaaaacac cttttcaaac gaaatttgtt tttgcattgg gacaacaaca
     4381 gctaaagaag tagaaaaaac agccaaaaac atagtgattg ctaaccagac aacagttgaa
     4441 aatgtaatta tacagagtat aaactatttt aaaaatgaga atagcaaata gaataacagc
     4501 ttacagcata ttgcctaaag cctaaagcaa aagataatga taaagaacga ccttttttta
     4561 agagccttaa ataatgaaac cgtggaacgt ccacccgttt ggatgatgcg tcaagccgga
     4621 agatatttac ctgaatttag agctttacgc gataaatatg atttttttac ccgttgcgaa
     4681 acacctgaat tagcggccga aattacggtt caacccattc gaattgtaaa acctgatgcc
     4741 gccattttgt tttcagatat tttggtggtg ccaagagcga tgggaattca tgtggaattg
     4801 aaagataatt taggacccat tattccgaat ccgattcgaa caatcgagca agtagaccaa
     4861 gtatatgttc caaacgtcaa tgaaacttta ggctatgttt ttgatgccat caaattaacc
     4921 aaagaaatgc tgaatgatga ggtgccgctc attggttttg caggttcacc atggaccatc
     4981 ttttgctatg cggtggaagg aaaaggctcg aagagttttg atacagccaa aggattttgt
     5041 ttttcaaatc cggtagcagc acatatttta ttgcaaaaaa ttaccgacac cacaatttta
     5101 tatttaaaag aaaaagtaaa agcaggttgt aacgccattc aaatttttga ttcttggggc
     5161 ggcatgcttt ctccggttga ttatcaagaa ttttcatggc gatacattaa ccaaattgtt
     5221 gaggcgttag ctccacacac caaagtaatt gtctttggaa aaggatgttg gtttgcattg
     5281 aatgatatgg caaaatcaaa agcatcggct ttaggtgtcg attggacttg ttctcctaga
     5341 aatgcccgtt atctatcagg aggaagaatt acgctccaag ggaatttcga tccatctcgc
     5401 ttattatcgc ccattccaac catcaaaaaa atggttcatg agatgattga tgaatttgga
     5461 aaagacaatt acatcgtcaa ccttggtcac gggattttac ccaatattcc ggtagatcat
     5521 gcgaaggctt ttgtggaagc agtgaaggag tataaaaagt aattttatga taaaaacaga
     5581 tttatttttt attgattatt tagtattaga agatgccgca agcctatctg aaatgatgtt
     5641 gtgtaatcac gataatttta aaagatattt cccgataact ttatctaaaa acaaatcaat
     5701 agaagatagt ttaaaattta ttaaagaaaa aaatatagaa tgccatgcca aatcggagtt
     5761 tacttttgta ataaaagaca aacaattgtc aaaagcagtt ggtataatta taatcaaaga
     5821 aataaattgg gaaatcaaac aaggtgaatt tgcttattgt atgagttcag aatttgaaaa
     5881 caaaggatgg atgtcaaatg cgattgcttt aacttccaaa tacgcatttg aagttttagg
     5941 ttttaataaa cttcaaatca ttgctcacag aacaaatttt ggaagttgta aagttgctga
     6001 aaaatgcggg ttccaatggc aaaagacttt aataaatgaa tatacaccac caaatgaatc
     6061 gccattagat atggaattat acgaattata caatgagaga taaattctat caatacattc
     6121 tccaattgca aaatcaaatc acttctaaat tagaagcaat cgatggtgtt gctaaattcc
     6181 gtgaagacat ttgggagcga ccggaaggcg gtggtgggaa aacaagagtt attgaaaacg
     6241 gcctagtttt cgaaaaagga ggcgtaaata tctcagctgt tcatggaaaa ttaccggact
     6301 ccatgcaaaa attattcaac gttggtgatg ctgatttttt tgcctgcggt ttgagtttag
     6361 tgattcatcc caaaagtcca atggtgccaa ccgttcatgc caattggcgg tattttgaaa
     6421 tgtatgatga
//
LOCUS       DAOTCW010000129         6270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_9150_length_6270_cov_40.764280, whole genome shotgun sequence.
ACCESSION   DAOTCW010000129 DAOTCW010000000
VERSION     DAOTCW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9150_length_6270_cov_40.764280"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="P5335_09545"
     CDS             <1..993
                     /locus_tag="P5335_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HRZ75163.1"
                     /translation="WQASSGASAQDVLSTISLTYSHSSDDRNTIWNAHFSGSVEYDYS
                     SIGFGGGLTKLFNDKNTEVGISANVYLDTWSPIYPKELQDFVDYGNSTNGGIFNYYTI
                     TGNTDYNPSGFSFHENKNRNSYALSLSFSQVLNKKMQLSVFVDFLQQQGLLSTPYQRI
                     YFADVADSFINEFQLADDIEQLPDTRFKIPIGARFNYYLNEKFVLRTYYRFYSDDWGI
                     SSHTASIELPYKITDRFTVFPMYRYYTQVESKYFAPYEAHLSTEEFYTSDYDLSTFDA
                     HQYGLGVSYTDIFTGAHVWKFGIKNIDLRYNHYSRSDGLEADIVSFGIKFILDK"
     gene            990..1652
                     /locus_tag="P5335_09550"
     CDS             990..1652
                     /locus_tag="P5335_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRZ75164.1"
                     /translation="MKLLVVEDEIGIASFLKQGLEEEGYDVVVTNDGEAGLKMASSQN
                     PDLILLDWMLPKLSGLEVCTSFRKTNTSTPIIFLTAKDTVQETIEGLKAGANDYIKKP
                     FSFDELLERIKIHFRNSDLEDIVSLGRIKIIRSKHQVLVDEKEVNLTQREYELILYLI
                     KNKGKVCTRNQIIEDVWDIHFEYDTGVIDVFMNAIRKKLNLTKEDECIKTIRGVGYIA
                     ND"
     gene            1667..3046
                     /locus_tag="P5335_09555"
     CDS             1667..3046
                     /locus_tag="P5335_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRZ75165.1"
                     /translation="MNSFSFRNRIAFYYIISTALLITVVFLVIYQMVSFSVFNHINAD
                     IEKEVEKHLEEIEIDRNDTYLIQVDQWKAREHNTVDVNPVFVQFYDLNNELIDKSPNL
                     KNLDLKLFAEEKENQLIDTYLNNKPIRQIQVPLFDDDKVIGHLVVAMSLDDSKMVLTN
                     LRTILVISFPLILIFLFLIARFIVGRSIKPIDAIIETSNQISRENLTTRINLPQNKDE
                     LYLLSKTINDLLDRVENAIDREKQFTSDASHELRTPLAVIKGTLEVLIRKPRNQAEYE
                     EKINFCVKEVDRLNQLVDELLLLARFEDQKQSIKHESVYLNSLLLDVITRSSEKIRTK
                     NIKLKTSFAKDFVITNDSYLISIIFSNLLSNAIKYSQENGTIFVNLENENNHIVCKIS
                     DNGIGISKEDLEKIGSRFYRSQKVEHAEIKGTGLGLSIVKRLTELLSIQFNIESQLKL
                     GTTVILRFY"
     gene            3120..>6270
                     /locus_tag="P5335_09560"
     CDS             3120..>6270
                     /locus_tag="P5335_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRZ75166.1"
                     /translation="MLEKIIAFSLKNKLIILLFTLAVFGFGIFSVFQISIGAVPDVTN
                     NQVQVITTSRNLSTEDIEQYITYPVELEMANLPSVKEIRSISKFGLSVVTIVFDDAIG
                     TYLPRQLIAEKIKSASEKIPQGFGTPEMGPITTGLGEIYQYTLEVKPEFKNQYSVTDL
                     RTIQDWIVKRQLSGIKGVVEINTWGGFLKQYEVAIVPSRLNAMNITTSEVFAALEKNN
                     SIAGGAYIEKVNQSYFIRGEGKVNSISDIENIVVKNKDGVPVYIKNVATVRFGHANRF
                     GAITGNGEGEKVLGQVMMLKGANSKQVIDDVKNRVTEIQKSLPEGVYINGFLERSELV
                     GKTTFTVAENLILGCLIVIFVVVLLLGNWRSGLVVASVIPLCLLFAISFMNIFGIDAN
                     LMSLGAIDFGIIIDGAVIIVEFIAFQIAQKSKHLVGMEKVEMQLEIDKITYKSASKMM
                     NSAIFGQLIILIVFIPILSLSGVEGKMFKPMAMTFSFALVGAMIFCFTYVPVVASLFL
                     KPREENPNSISNKLINKLNSWYLPVITWALSNTKKVLYGALGLLLLAVGLFATMGGEF
                     IPTLDEGDFVIQPVLKTGTSLTKTIETTTKIESIILKNFPEVDQVVSRIGAAEVPTDP
                     MSMEESDIIVKLKPKSDWVSASSKDELADKIKAAIETKIPNMEIEFTQPIEMRFNELI
                     SGTRSDVAVKIFGDDLDILAKKANEVAIAIKNIEGASDVIIEKTEGLPQMAVQFDRSK
                     IARYGLNIADLNELIALGFAGKTVGTVFEGAKRFDLVIRLDKTNRKDIEDLRNLYVST
                     PNGEQIPLRELATIEYTEGPAKISRDNTNRRIVVGINVRNRDLQSVVDDIQKIVDTKI
                     NLPSGYYVTYGGQFENLQSAKARLLIAVPIALFLIFILLHFAFGSIKEALMVYSAIPL
                     SAVGGVLFLWMRDLPFSISAGVGFIALFGIAVLNGIVLIEHFKELKHQGMTNMNELIL
                     KGTTDRLRPVLLTAAAAALGFLPMAISSSAGAEVQRPLATVVIGGLFTATLLTMIVLP
                     ILFKVLDEKEFKKPKFKKHH"
BASE COUNT         2098 a          964 c         1141 g         2067 t
ORIGIN      
        1 tggcaagcta gttcaggtgc ttctgctcaa gacgtattgt caacaatttc tcttacatac
       61 agtcacagtt ctgatgatcg taatacaatt tggaatgccc atttttcagg ttctgttgaa
      121 tatgattatt catctatcgg ttttggtggt gggttgacta aacttttcaa tgataaaaat
      181 acggaagttg gaatttctgc caatgtttat ctcgatactt ggagtccaat ctatccaaaa
      241 gaattacagg attttgtaga ttatggcaat tcaacaaatg gaggtatttt taactattat
      301 acaataaccg gtaataccga ttataatcca tccggatttt catttcacga aaataaaaat
      361 agaaattcct atgccttatc acttagtttt tcgcaagtgt taaacaaaaa aatgcagtta
      421 tccgtttttg tggatttttt gcaacaacaa ggtttattgt ctacacctta tcaacggatt
      481 tattttgctg atgtagctga ttcattcatt aatgaatttc aattagcgga cgatattgaa
      541 caattaccgg atacaagatt taaaatccca ataggagctc gattcaatta ttatctgaat
      601 gaaaaatttg tattacgaac ctattatcgt ttttattcag atgattgggg aattagttct
      661 cacacagcta gtatcgaact gccttataaa atcacggatc gctttactgt ttttccaatg
      721 taccgttatt atacgcaagt ggaatctaag tattttgctc cttatgaagc tcatctatcg
      781 acagaagaat tttatacttc cgattatgat ttgtctacct ttgatgctca tcaatacgga
      841 cttggggtaa gttataccga tatttttacc ggtgctcacg tatggaaatt tggaattaaa
      901 aatatagatt taagatataa tcattattca agaagtgacg gattagaagc tgatattgtc
      961 tcttttggaa ttaaatttat tttggataaa tgaaattatt agttgttgaa gacgaaattg
     1021 gaattgcctc ttttcttaaa caaggtttag aagaagaagg ctatgatgtt gtggtaacga
     1081 atgatggtga agctggttta aaaatggcat cttcacaaaa tccagattta atattattag
     1141 attggatgct tccaaaactt tccggattag aagtttgtac atcttttcga aaaacaaaca
     1201 catcaacacc gattattttt ttaacggcta aagataccgt tcaggaaacc attgaaggat
     1261 taaaggccgg agcaaatgac tatatcaaaa aaccttttag ctttgatgag ttgctggaac
     1321 gaattaaaat tcattttaga aatagtgatt tagaagatat agtatcgtta ggtagaatta
     1381 aaattattcg ttcaaaacac caagttcttg ttgatgagaa agaggtaaat ctcacacagc
     1441 gtgaatatga gttaatcctt tacttaatta aaaacaaagg aaaagtttgc acccgaaatc
     1501 aaatcattga agatgtttgg gatattcatt ttgaatatga taccggtgtg attgatgtat
     1561 tcatgaacgc cattcgtaag aaattaaacc ttactaaaga agatgagtgt attaaaacaa
     1621 ttcgtggagt aggatatatt gccaacgatt aaattaaaat tcattcatga actctttttc
     1681 attcagaaat agaattgctt tttattatat tatcagtact gcgttattga ttacagtagt
     1741 ttttttggta atatatcaaa tggtaagttt cagcgttttt aaccatataa atgctgatat
     1801 tgaaaaagaa gttgaaaaac acctagaaga aattgaaatt gatcgtaatg acacttacct
     1861 tattcaggtt gatcaatgga aagcaagaga acataacaca gttgacgtaa atcccgtttt
     1921 tgtccaattt tatgatttaa acaatgaatt aattgataaa tcacctaatt taaaaaacct
     1981 cgatttaaaa ctttttgcgg aagaaaagga aaatcaactg attgatactt atcttaacaa
     2041 taaaccaatt cgtcaaattc aagtcccgct ttttgacgat gataaagtca ttgggcactt
     2101 agttgtagcg atgtctttag atgactcaaa aatggttttg actaatctcc gaacgattct
     2161 tgtcatttca tttcctttaa ttttaatttt cttgtttttg attgcccgtt ttattgtcgg
     2221 tagaagtata aaacctattg atgccattat tgaaacatct aaccaaatta gtcgtgaaaa
     2281 tctaactaca cggattaatt tgccacaaaa taaagatgaa ttatatttac tttccaaaac
     2341 cataaacgat ttactagacc gtgtagaaaa tgccatagat agagaaaaac agtttacttc
     2401 tgatgcttca catgaattgc gaactccttt agcagttatt aaaggaacat tggaagtact
     2461 tattagaaaa cctcggaacc aagccgagta tgaagaaaaa atcaattttt gtgttaaaga
     2521 agtagacagg ctaaatcaat tagtcgatga attattgctt ttggcaagat ttgaagacca
     2581 aaagcaaagt ataaaacacg aatcagtata tctaaattcc ttactgttag atgttataac
     2641 ccgttcctca gagaaaatca gaactaaaaa cattaaacta aaaacatcct ttgcaaaaga
     2701 cttcgtaatt acgaatgaca gttatttaat ttcaattatt ttcagcaatt tactttcaaa
     2761 tgctataaaa tattcacaag aaaatggaac aattttcgta aatttagaga atgaaaataa
     2821 tcatatagtt tgtaaaatta gtgataatgg aattggaata tctaaagaag atttagaaaa
     2881 aattggtagt cgtttctatc gttctcaaaa agttgagcat gcagaaatta agggcacagg
     2941 actaggttta tctatagtga aaagattaac agagttactg tcaattcaat ttaatatcga
     3001 aagccaactt aaacttggaa caacagtgat attaaggttt tattaatgaa ttcctaaggc
     3061 tttcttaagt tttttccttt ttttttcgtt taattttaca ataataaaaa tcgaaaaaaa
     3121 tgctcgaaaa aataattgcc tttagtttaa aaaacaaact cattatatta ctttttacct
     3181 tagctgtttt tggctttggt attttctcag tatttcaaat ttccatcgga gcagttcctg
     3241 atgttaccaa taaccaagtg caggtaatta ccacttcacg caacctttct acggaagata
     3301 ttgagcagta tattacctat ccggtagaat tggaaatggc taatttgcct agtgtcaaag
     3361 aaatccgctc tatttcaaaa tttggattgt ccgtcgtcac catcgttttt gatgatgcca
     3421 ttggtactta tctaccacgt caattaattg cagaaaaaat taaatcagcc tcagaaaaaa
     3481 ttccacaagg atttggtact ccggaaatgg gtcccattac aaccggttta ggtgaaattt
     3541 atcaatatac acttgaagta aaacccgaat ttaaaaatca atattccgta actgatttgc
     3601 gtaccatcca agattggatt gtaaaacgtc aattatcagg tataaaaggg gtggttgaaa
     3661 ttaatacttg gggtgggttt ttaaaacaat atgaagtggc catcgttcca tcgcgactaa
     3721 atgcaatgaa tataactact tccgaagtgt ttgctgcttt agaaaaaaat aacagcattg
     3781 ccggcggagc ttatattgag aaagtaaatc aaagctactt tattagagga gaaggaaaag
     3841 taaatagtat cagcgatatt gaaaatattg ttgttaaaaa taaggatgga gttccggttt
     3901 atatcaaaaa tgtagcaacc gtaagatttg gtcatgccaa tcgttttgga gctatcaccg
     3961 gaaatgggga aggtgaaaaa gtacttggtc aggtcatgat gctcaaagga gctaattcaa
     4021 aacaggtgat tgacgacgtt aaaaatcgcg tgactgaaat tcaaaaatcg ttacccgaag
     4081 gtgtttacat caatggtttt ttagaacgta gtgaattagt tggaaaaacg acgttcaccg
     4141 tggccgaaaa tttaattctc ggttgtttaa tcgtcatttt tgtcgtggta cttttacttg
     4201 gaaattggcg ttctggttta gtggtagctt ccgtgatacc gttgtgttta ctatttgcca
     4261 tttccttcat gaatattttc ggaatcgatg caaatttaat gagtttgggg gcgattgact
     4321 tcggaattat tattgatgga gctgtaatta ttgtggaatt tattgctttt caaattgctc
     4381 agaaatccaa gcatttagta gggatggaaa aagttgaaat gcaattagag attgataaaa
     4441 ttacgtataa aagtgcttct aaaatgatga attcggccat ttttggtcag ttgattattt
     4501 taattgtttt tattccaata ctttctcttt caggtgttga aggaaaaatg tttaaaccta
     4561 tggcaatgac gtttagtttt gccctagtgg gtgctatgat tttttgtttt acctatgttc
     4621 cggtagtggc ctctttattc ttaaaaccaa gagaagaaaa ccccaattcc atttctaata
     4681 aactgattaa taagttaaat tcttggtacc ttcctgtaat tacttgggct ttgtcaaaca
     4741 ctaaaaaagt attgtatggt gccttgggat tattattgtt ggcggttgga ttatttgcca
     4801 ccatgggagg agagtttatt ccaacactcg acgaaggtga ttttgttatt caaccggttt
     4861 taaaaacagg gacttcctta accaaaacca ttgaaaccac aactaaaatt gagagcatca
     4921 tccttaaaaa tttccctgaa gtcgaccaag tagtgagtag aataggagct gcggaagtac
     4981 ccaccgaccc aatgtcgatg gaagaaagtg atataattgt caagttaaaa cctaaatcag
     5041 attgggtttc tgcttcttca aaagatgaat tagcagataa aatcaaagcc gcaatcgaaa
     5101 ctaaaattcc aaacatggaa atcgaattta cgcaacccat cgaaatgcgt ttcaatgaat
     5161 tgatttccgg aacacgctct gatgtagccg taaagatttt tggtgatgat ttagatattt
     5221 tggctaaaaa agccaatgaa gtagccattg caattaaaaa tatcgaagga gcctccgatg
     5281 taattattga aaaaacagaa ggtttacctc aaatggcggt gcagttcgac agaagtaaaa
     5341 ttgctcgtta tggattaaat atagccgatt tgaatgaact gattgcgttg ggtttcgcag
     5401 gaaaaacagt aggaactgtt tttgaaggtg ccaaacgctt tgatttggtt attcgtttag
     5461 acaaaactaa ccgaaaagat attgaagatt tacgaaattt atatgtttct acacccaatg
     5521 gtgaacaaat tccacttcgt gaattagcaa ctattgaata caccgaagga cctgcaaaaa
     5581 tttcacgtga taataccaac cgcagaattg tggtgggaat caatgtcaga aaccgcgatt
     5641 tacaaagtgt ggttgatgac attcagaaaa ttgttgacac aaaaattaat ttgccttcgg
     5701 gttattatgt aacctatggt ggtcaatttg aaaaccttca aagtgccaaa gcccgattgt
     5761 taattgccgt tcccattgcc ttatttttaa tttttatttt actgcatttt gcttttggtt
     5821 ccatcaaaga agcattgatg gtttattctg ctattccttt gtctgcggtg ggtggtgttt
     5881 tgtttttgtg gatgcgtgat ttacctttta gtatttccgc aggcgttgga ttcattgctc
     5941 ttttcggaat cgcagtttta aatggtattg ttttgattga acattttaaa gaactaaaac
     6001 accaaggaat gaccaatatg aatgaactta ttttaaaagg gacaacagac cgattacgac
     6061 cggttttatt aactgccgca gcagctgcac tcggattttt accgatggca atctcttcat
     6121 cagcaggagc agaggtgcaa cggccgttgg caacagtagt cataggcgga ttgtttacag
     6181 ctactttgct gacaatgatt gtattaccta ttttatttaa agtattggat gaaaaagagt
     6241 ttaaaaaacc taaattcaaa aaacaccata
//
LOCUS       DAOTCW010000130         6259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_9173_length_6259_cov_49.723082, whole genome shotgun sequence.
ACCESSION   DAOTCW010000130 DAOTCW010000000
VERSION     DAOTCW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9173_length_6259_cov_49.723082"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1387
                     /gene="purD"
                     /locus_tag="P5335_09565"
     CDS             113..1387
                     /gene="purD"
                     /locus_tag="P5335_09565"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRZ75167.1"
                     /translation="MTILLLGSGGREHALAWKMLQSSKCTKLFVAPGNAGTATIASNV
                     SINPTDFDAIKNFVLQEKVEMVVVGPEDPLVLGIYDFFKNDDKLCTIPVIGPSKEGAK
                     LEGSKEYAKEFLIKHKIPTAAYDSFTAKTVENGCKFLETLSPPYVLKADGLAAGKGVV
                     ILNDLAAAKKELRDMLVHQKFGKASSKVVIEEFLDGIELSCFVLTDGKNYKILPTAKD
                     YKRIGEGDTGLNTGGMGAVSPVPFADEILMEKIETRIVKPTIEGLQKDGIEYKGFVFI
                     GLINVKGEPIVIEYNVRMGDPETEVVIPRIKSDLVELFLAVANEKLNEIILEIDQRSA
                     TTIMVVSGGYPEEFEKGKIISGISAVKDSIVFHAGTKFENDTVVSNGGRVLTVTSYGN
                     DFQEAIKKSYQNIEKLHFDKMYYRKDIGFDLL"
     gene            complement(1379..1609)
                     /locus_tag="P5335_09570"
     CDS             complement(1379..1609)
                     /locus_tag="P5335_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRZ75168.1"
                     /translation="MKAFFEGIQTLFVDFLFIPFDFFRSLELSSWWGANTLNFIFMLV
                     CFVSLVYWTLQLKQHKANNEENQDTTAHSFLK"
     gene            1656..2597
                     /locus_tag="P5335_09575"
     CDS             1656..2597
                     /locus_tag="P5335_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75169.1"
                     /translation="MIASVFSKSRPFNYILITSLLVFCYFIYQIKHVISTEDSSLTLF
                     DVIQKGTVLLLVTASLFITNFITKRNGLSKDNSYSFLFYFAFLLFIPSVFNKINLILA
                     SFFILLALRRLISMKSLITPKEKIFDASLWIFIATLFHFWSILFIVIVFASIIFHVSS
                     DYRNWILPFIAFFTVGVIFLFFSLLIDKEWISIVWKQIDLNFDFHYFSNSVENLALTL
                     FIIFSVFFLFAMLLTLSRRPLILHASYKKIIFSLLISLLIYVFSPVKSNDLLIFSILP
                     LSILATAYLEFIRDSMLKEIITSSIVITGLLMFMFQL"
     gene            complement(2592..3245)
                     /gene="upp"
                     /locus_tag="P5335_09580"
     CDS             complement(2592..3245)
                     /gene="upp"
                     /locus_tag="P5335_09580"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRZ75170.1"
                     /translation="MHLHYISEQNSILNHFLAQIRDVTVQKDSMRFRKNIERIGEIMA
                     YELSKILPYKNIEVQTPLGIKKTTALEDHVVLCSILRAGLALHTGFMNIFDNAENGFV
                     SAYRHHPNNDDYFDILVEYQAAPSFENKTLILIDPMLATGQSIVAVLNKLHLDQNPKE
                     IHIAVVIAAPEGIAYLEKNLPSNCHLWVAALDDKLNEKNYIVPGLGDAGDLAYGVKL"
     gene            3315..3920
                     /locus_tag="P5335_09585"
     CDS             3315..3920
                     /locus_tag="P5335_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HRZ75171.1"
                     /translation="MFSQIAFSVFEQSIVDYHIHNNVDQPINNPYAKDKIEHLLYAKN
                     WVDTVQWHYEDIIRDPQINPVAALVLKRKIDASNQVRTDMVEYIDSYFLQKYSGVSVK
                     ADAKINTESPAWAFDRLSILALKIYHMNEEATRVEASAEHRAKCQEKLNVLLEQKSDM
                     FSSIDQLLKDIESGDKFMKVYKQMKMYNDEELNPVLYQNKK"
     gene            4045..5049
                     /locus_tag="P5335_09590"
     CDS             4045..5049
                     /locus_tag="P5335_09590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRZ75172.1"
                     /translation="MGDVAMTVPVIRAFVKQYPDVKLTIVSRPFFKPFFEGIPNVDFF
                     SVDVKRRHKGFLGILKLYSDLKKLNIDAVADLHNVLRSNVVRTLFAVSGKKVAFTDKG
                     RVEKKALTSLTNKVFKPLQPMVERHAITFSELGFPLDLKSPEFPPKASLSNEISSIIV
                     DTPSSIGIRWIGIAPFAQYDSKVYPSDLMQKVIDVLAENKSNKILLFGGGSKEIEQLN
                     RLQNNHSNVIVVAGKLNFQEELKLISNLNVMLSMDSGNAHIAAMLGVKVITLWGATHP
                     FAGFSPFNQPLENCITADREKFPLIPTSVYGNKKIADYEDAMRSIEPEKIISKVNNLL
                     "
     gene            complement(5060..6112)
                     /locus_tag="P5335_09595"
     CDS             complement(5060..6112)
                     /locus_tag="P5335_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HRZ75173.1"
                     /translation="MSTFFKLSVKEIKRETPEAVSILFHVPTEIQENYQFVAGQYINL
                     KLTLDGIEIRRAYSLCSSPESGDLRIAVKAVKNGYFSKFANDQLTIGKVLEVGTPEGK
                     FTFEPDASKQKNYAAFVAGSGITPVMSILQTVLEKEPNSTFILVYGNKSIPDTIFYNQ
                     LHELQQKYVGRFFVHYVFSQQKIDDQLFGRIDKSAVNFVLKNKHKEKDFDKFYLCGPE
                     EMINLVTSVLKENNIKEKDIKFELFSASSSENEISKSLEGHTKITILLDDEETSFEMS
                     QKQNVLEAALKQGLDAPYSCQGGICSSCLARITKGTAEMKKNTILTDKEIAEGLILTC
                     QAYPTSSEIFVDYDDV"
BASE COUNT         2086 a          947 c         1065 g         2161 t
ORIGIN      
        1 tcaaaaataa taaaaccttt aaccgaaagc tatattctca cacttttttt gcaatatcgt
       61 tttaacttct atttaaatca ccttattttt gcactactaa caacaacaca acatgacaat
      121 tttattactt ggttccggcg gaagagaaca tgctttagct tggaaaatgc ttcaaagttc
      181 aaaatgcact aaactttttg tggctcctgg aaacgcagga actgccacaa tcgcctcaaa
      241 tgtttcaatt aatccaaccg attttgacgc aattaagaat tttgtattgc aagaaaaagt
      301 agaaatggtt gtcgttgggc ctgaagatcc attggtttta ggaatttatg atttctttaa
      361 aaatgatgat aaattgtgta caattccggt tataggacca tcaaaagagg gggcaaaact
      421 agaaggaagt aaagaatatg ctaaagaatt tttaattaaa cataaaattc caactgctgc
      481 ttatgatagt tttactgcca aaacagttga aaatggatgt aagtttcttg aaacactttc
      541 tcctccttac gttttaaaag ctgacggttt agccgcagga aaaggggttg taattctgaa
      601 cgatttagct gcggctaaaa aagaattgag agatatgctc gttcatcaaa aatttggaaa
      661 agccagttca aaagtggtaa ttgaagaatt tctggatggt attgaattga gttgttttgt
      721 attaaccgat ggtaaaaact ataaaattct tccgactgct aaagattata aacgaattgg
      781 cgaaggagat accggcttga ataccggagg aatgggagct gtttcaccgg ttccttttgc
      841 agatgaaatc ttgatggaaa aaatcgagac aagaattgtt aaaccaacta ttgaaggcct
      901 tcaaaaagat ggaattgaat ataaaggatt tgtttttatt ggcttaataa acgtaaaagg
      961 agagccaatt gtcattgaat ataatgttag aatgggtgat ccggagacag aagttgtgat
     1021 tcctcggatt aaatcggatt tggttgagtt gtttttagcg gttgccaatg aaaaattaaa
     1081 tgaaattatt ttagaaattg atcaaagaag tgccacaaca attatggttg tttctggagg
     1141 atatccggaa gaatttgaaa aaggcaaaat aatttccggt atttcagcag tgaaagattc
     1201 tattgttttt catgccggga caaaatttga aaatgacaca gtagtttcta atggtggtag
     1261 agtactaacg gttacctctt atgggaatga ttttcaagag gccataaaaa aatcttacca
     1321 aaacatagaa aagctacatt ttgataagat gtattataga aaagatatcg gtttcgactt
     1381 actttaaaaa agagtgagcc gttgtatctt gattttcttc gttgttagct ttatgttgtt
     1441 tcaattgtaa agtccaataa acaagtgaaa caaaacaaac aagcatgaat atgaaattaa
     1501 gcgtattggc accccaccag cttgataatt ctaaacttct gaaaaaatca aaaggaatga
     1561 ataaaaagtc tacaaataag gtttgaattc cttcgaaaaa tgctttcatg gtataaagta
     1621 ttatatttac aatcgcaaaa gtataaaata tccttatgat agcaagtgtt tttagtaaat
     1681 caagaccatt taattatatt ttaattacta gtttgttggt tttttgctat ttcatttatc
     1741 aaatcaagca tgtaattagt acggaagatt catctttgac gctttttgat gtgattcaaa
     1801 aaggcacggt tttacttttg gtaaccgcct cattattcat tacaaatttc attacaaagc
     1861 gaaatggatt gagcaaagac aatagttatt cttttttatt ttactttgct ttcttactat
     1921 tcattccttc agttttcaac aaaataaatt taattctggc ctcttttttt attttattgg
     1981 cattacgtag attaatttct atgaaatcgt taattacgcc taaagaaaaa atatttgatg
     2041 cctcattatg gatatttata gcgacacttt ttcatttttg gagtatttta tttattgtaa
     2101 ttgtttttgc ctctattatt tttcacgttt ccagtgatta tagaaattgg attttacctt
     2161 ttattgcttt ttttactgta ggagtgatat ttttattttt ttcgttacta atagataaag
     2221 aatggataag tattgtttgg aaacaaatag atctaaactt tgattttcat tattttagta
     2281 attctgtaga aaatctggct ttgacacttt ttattatttt ttcagtattt tttctattcg
     2341 cgatgctact tactctgtcg agaagacctt taattttaca tgcatcttat aaaaaaataa
     2401 ttttcagctt attaatcagc ttgttaattt atgttttttc tcccgtaaaa agtaatgatt
     2461 tgcttatttt cagcatttta ccactttcca ttttagctac agcctatctt gaatttatca
     2521 gagattcaat gttaaaggaa attattacaa gtagcattgt aatcacgggg ttattaatgt
     2581 ttatgtttca attataattt aacaccatag gctaaatcac cggcatcgcc taaaccaggc
     2641 acgatatagt ttttttcatt taatttatca tctaaagcag ctacccataa atggcaatta
     2701 gaagggagat ttttttctag ataagcaatt ccttctggtg cagcaatcac taccgcaata
     2761 tgaatttctt ttggattttg atctaaatgt agtttgttta aaaccgcaac aattgattgc
     2821 ccggtggcta acatagggtc aattaaaatc aaggttttat tttcaaacga tggagcagct
     2881 tggtattcca ccaaaatgtc aaaataatca tcgttgtttg gatggtggcg ataggctgat
     2941 acaaatccgt tttcagcatt gtcaaaaata ttcatgaaac cggtgtgcaa cgccaaaccg
     3001 gcccttaaaa tggaacataa aacaacatga tcctccaaag ctgttgtttt tttaatgcca
     3061 agcggagttt gtacttcaat gtttttgtaa ggcagtattt tgctcaattc atatgccatg
     3121 atttcaccga tgcgttcgat atttttacga aatcgcatac tgtctttttg tactgtaaca
     3181 tcgcgaatct gtgccaaaaa atgattgaga atactgtttt gttcggagat ataatgaaga
     3241 tgcataaacc ttttgaattg gaattagttt gataaaggta taaaaagtat atttgtattt
     3301 taaaattaat aggtatgttt tcacaaatag cattttctgt tttcgaacaa agtattgttg
     3361 attatcacat tcataacaat gtagatcaac cgattaacaa cccgtatgcg aaagataaaa
     3421 ttgagcattt attgtatgca aaaaattggg tagacactgt tcaatggcat tatgaagaca
     3481 ttattcgtga tccacaaatt aatccggtgg cagctttggt tttaaaaaga aaaattgatg
     3541 catcaaatca ggttcgtact gatatggtag aatatattga tagttatttt cttcaaaaat
     3601 acagcggtgt ttctgtcaaa gcagatgcaa aaatcaatac cgaaagtccg gcatgggcat
     3661 ttgaccgcct ttctattttg gctttaaaaa tttatcacat gaatgaggaa gctactcgag
     3721 tagaagcttc ggctgaacat agagcaaaat gtcaagagaa attaaatgta ttattagaac
     3781 aaaaaagtga tatgttttca tccatagacc aattactgaa agatattgaa agtggtgata
     3841 aattcatgaa agtctacaag caaatgaaaa tgtacaatga tgaagaattg aatcctgttt
     3901 tgtatcagaa taaaaagtaa attatttttg aaccattaag gaattaagaa aaattaagca
     3961 tcaaatctta atggttttta atttaaaaaa aatcattaat tgtctcaaaa accaaaacat
     4021 atcgtcgtta tccgtctttc cgccatgggc gatgtcgcta tgacggtccc ggttataaga
     4081 gcatttgtaa aacaatatcc tgacgttaaa cttaccattg tttcccgacc tttttttaaa
     4141 cctttttttg agggtattcc gaatgttgat tttttttctg tcgatgtaaa aaggaggcac
     4201 aaagggtttt tagggatact caaattatat tccgatttaa aaaaattgaa tattgatgca
     4261 gttgctgatt tgcataatgt tcttcgttca aatgtagttc ggacattatt tgcggtcagt
     4321 gggaagaaag tagcttttac ggataaagga agagttgaaa aaaaggcttt aacaagtctc
     4381 acaaataaag ttttcaaacc tttacaaccg atggtggaac gccatgcaat tactttttca
     4441 gaacttggtt ttcctttaga tttaaagagt ccggaatttc ctccaaaggc ttctttatcg
     4501 aatgaaattt catcaattat tgtcgatact ccatcttcaa ttggaataag atggattgga
     4561 atcgcaccgt ttgctcaata cgacagcaaa gtctatcctt cggatttaat gcaaaaagtg
     4621 attgatgtat tagccgaaaa taagtcaaat aaaattttac tttttggagg aggtagcaaa
     4681 gaaattgagc aattaaatcg attgcaaaac aatcattcca atgtaattgt tgttgcagga
     4741 aaactaaact ttcaagaaga gttaaagttg atttctaatt taaatgttat gctttccatg
     4801 gatagcggga atgctcatat tgccgcaatg ttgggcgtaa aagttatcac cctttgggga
     4861 gccacacatc cgtttgcagg tttttcgccg tttaatcaac cgttagaaaa ttgcattaca
     4921 gctgatagag aaaagtttcc gttgattcca acatctgttt atggcaataa aaaaattgcg
     4981 gattatgaag acgcgatgag aagtatagaa ccggaaaaaa taattagtaa agtaaataat
     5041 ttactttaga ggcagttcgt tacacatcat cataatctac aaaaatctca ctcgaagttg
     5101 gataagcctg acaggttaaa atcaatcctt cggcaatttc tttgtcagtt aaaatagtgt
     5161 tctttttcat ctctgcagtt ccttttgtta tgcgggctaa acaactacta caaatacccc
     5221 cttgacagga ataaggagca tctaaacctt gttttaaagc tgcttctaat acgttttgtt
     5281 tttgtgacat ttcgaaagaa gtctcctcgt catccagtaa aatggtaatt ttagtgtgac
     5341 cttctaacga tttactgatt tcattctctg aagaagaagc ggaaaataat tcgaatttaa
     5401 tatctttttc tttgatatta ttttctttta aaacactagt cacaaggttt atcatttctt
     5461 caggcccaca taaatagaat ttatcaaaat ctttttcttt gtgtttgttt ttcaaaacaa
     5521 aatttacagc tgatttatct attcttccaa ataattggtc atctatcttt tgttgactaa
     5581 acacataatg aacaaaaaaa cggcctacat atttttgttg taattcatga agttgattat
     5641 aaaatatagt atccgggata cttttatttc catataccaa tataaatgtg ctatttggct
     5701 ctttttctaa tacggtttga aggattgaca ttaccggtgt aattccactt ccggccacaa
     5761 aagcagcata atttttttgt ttggaagcat cgggttcaaa agtaaatttt ccttcaggag
     5821 tgcctacttc caaaacttta ccaatcgtta attgatcatt agcgaatttt gaaaaataac
     5881 catttttaac ggctttaact gcaatacgca aatcaccact ttccggagaa gagcataaag
     5941 aataagcacg tctaatctcg attccgtcta acgtaagttt taaattaatg tattgccccg
     6001 ctacaaattg gtagttttct tgaatttcag taggaacatg aaaaaggatg gaaacagctt
     6061 ccggtgtttc gcgtttaatt tctttaacag ataatttaaa aaaagttgac atggtatttt
     6121 ttttgcaaag ttacgaatct gaaagatttt gattgagttt gaaaactatt ttttcagcat
     6181 tgtagtaaca aaaaagattt tagactacta actttataat tcataaaact caacttgttt
     6241 atgttcaaac attttatca
//
LOCUS       DAOTCW010000131         6191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_9319_length_6191_cov_37.063233, whole genome shotgun sequence.
ACCESSION   DAOTCW010000131 DAOTCW010000000
VERSION     DAOTCW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9319_length_6191_cov_37.063233"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="P5335_09600"
     CDS             complement(<1..552)
                     /locus_tag="P5335_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HRZ75174.1"
                     /translation="MNILLELNLSKAIRLILTKTSYMEKYVIKRNGAFLPFEAFKIEE
                     AIQKAFQSESQEYDQAVFNKVVILLERKETWSVEEIQDTIEKVLFEFGHYSVMRSFML
                     YRHTRKLQREHILGLNDDTTYVDSSQTIEEYISQTDWRINANANTSYSNAGLVNNVAG
                     KIIANYWLDKVYSKEEGYAHRNGD"
     gene            complement(695..898)
                     /locus_tag="P5335_09605"
     CDS             complement(695..898)
                     /locus_tag="P5335_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75175.1"
                     /translation="MKKLVSLMAVAAFLFTSSAFAGEPKEKEKKKEKAKTEKSCSTEE
                     KKSCGTEKKGGCCSSKKAEEKKA"
     assembly_gap    1162..1261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1262..2544)
                     /locus_tag="P5335_09610"
     CDS             complement(<1262..2544)
                     /locus_tag="P5335_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRZ75176.1"
                     /translation="MKIEQIYTGCLAQGAYYIESKGEVAIIDPLREVQPYLDKAEQNN
                     AKIKYIFETHFHADFVSGHVTLSEKTNAPIIFGPTATPTFKAHIAKDGEVFKLGEITI
                     TTLHTPGHTMESTTYLLKDKNGKDYAIFSGDTLFLGDVGRPDLAQKAADMTQEQLAGL
                     LYDSLRTKIMTLADDVIVYPAHGAGSACGKNLSKETVGSIGDQKATNYALRANMTKEE
                     FVKEVTDGLLPPPAYFPMNVQMNKEGYSNVENIIKEAKAFEPKVFETVANETEALVLD
                     VRHQDDFAKGHIPKSIFIGLDGGFAPWVGALILDYKQPILLVTPEGKENETITRLARV
                     GYDNAIGYLKGGFETWKKAGMEYDTVTTVGPEVLEENMKLHVPVFDVRKPGEYASEHI
                     VDVPSAPLDFLNEHLVQFPKNEPFYIHCAGGYRSM"
     gene            complement(2682..4097)
                     /locus_tag="P5335_09615"
     CDS             complement(2682..4097)
                     /locus_tag="P5335_09615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015022847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRZ75177.1"
                     /translation="MKIEQIYTGCIAHAAYYLENNGEAAIFDPLREVQPYIDRARKDN
                     AKIKYVFETHFHADFVSGHLDLAKKEGAIIVYGPTAKPGFEALVAEDNQVFKVGNYQV
                     KVLHTPGHTMESTTYLLIDETGKEHGIISGDTLFIGDVGRPDLAQHVVADLTQDKLAR
                     HLFHSLRTKIMPLSDDLIVYPNHGAGSACGKMMSKETTDTLGNQKKTNYALRPDMTED
                     EFVKELLTGLTNPPGYFPKNVLMNIQGYESLDLVMERGKKAFNPDEFEVVANETRALI
                     LDTRRPEDFAKGFIPNSINIGLESNFAMWVGELIADIKQEILLVTYPGREEESIIRLS
                     RVGYDHAIGYLDGGFDSWVKSGKEIDQSNRITAEELAVQMGHKKVILIDVRKKSEYLS
                     EHVENAINIPLNEISNRMAEIPKDKPFVLSCAGGYRSMIAASILKQRGFENFTDVIGG
                     FSEIKKTTVPITDYVCPTTLL"
     assembly_gap    4191..4290
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4291..4601)
                     /locus_tag="P5335_09620"
     CDS             complement(<4291..4601)
                     /locus_tag="P5335_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PorT family protein"
                     /protein_id="HRZ75178.1"
                     /translation="MKKLIFAIIVLFSFSTVQAQVVQFGLKGGYNSATLSGLDGNKAL
                     SGFHVGALAEIDIVFMSIQPELVYSTQGSAFDSGDDVKLDYLNVPVMAKLNLLKILYL
                     EA"
     gene            complement(4722..5504)
                     /locus_tag="P5335_09625"
     CDS             complement(4722..5504)
                     /locus_tag="P5335_09625"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRZ75179.1"
                     /translation="MINKFSYIFLAKTICIIVIFFFNSFNLFSQTENQFTNEFQFNRN
                     IGKLTAMEFDFAQGFTSGEEENNPFYKNSQISARLWLHYFANKKWKLSGFAAFFYNRD
                     VPEISQKKSPEVRFAFQGIYYINRTDYVLTNRLRIEDRILGNAEGTVDIVYRFREMIK
                     LVYPISYVQIGKGSLYGIVSEELVFKTKGSTTGEGFFDRNRLTLGLGYSFTDTIQFEL
                     TYMNEYLPRNASDKMYNVLSTTLIFNDLIYALKRKSGSIQAY"
     gene            complement(5541..>6191)
                     /locus_tag="P5335_09630"
     CDS             complement(5541..>6191)
                     /locus_tag="P5335_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRZ75180.1"
                     /translation="EMVKANEYVVVNIPSYKLFFVRNGKQELVSPVVVGKAMNKTVIF
                     SGTMSYIVFSPYWNVPTSIIKKEIKPAMAKNKNYLASHNMEWNNGNIRQKPGKNNSLG
                     LVKFIFPNSNNIYLHDTPAKSLFEKETRAFSHGCIRVGKARDLALTILKDDPNWTTEK
                     IDAAMNSGKENWYTLKNKIPVYIGYFTAWVNANGEINFYDDIYERDERLLKLIYTE"
BASE COUNT         1950 a         1125 c          814 g         2102 t
ORIGIN      
        1 gtcaccattt cgatgagcat atccttcttc tttagagtac actttatcta accaataatt
       61 agcaattatt tttccggcaa cattgtttac caaaccggca ttagaataag atgtattggc
      121 atttgcatta attcgccaat cggtttggct gatgtattct tcgatagttt gtgagctgtc
      181 aacataggtc gtatcgtcgt ttaaacctaa aatatgttcc cgttgtaatt ttcttgtgtg
      241 acgatacaac ataaacgaac gcatcacgct gtaatggccg aattcaaaca gtactttttc
      301 aattgtatcc tgaatttctt cgaccgacca agtttctttt ctctctaata aaataactac
      361 tttattaaaa actgcttggt catattcctg tgattcactc tgaaatgctt tttgaatggc
      421 ttcttctatt ttgaaagctt caaacggcag aaaggcaccg tttcttttga taacatattt
      481 ttccatataa cttgttttgg tgagaattaa acgaattgct tttgacaaat ttaattctaa
      541 taggatattc atatccgttt tgaaaataaa gtaatgaaca agttatattt aattattgtt
      601 gataatttat taaatcgtaa taaaacaaga ataaaataat aacaaaaaaa aagtgctcga
      661 ttgctcgaac actttaaaat aacaacttaa gttattatgc ttttttctcc tcagcttttt
      721 ttgaagaaca acatcctcct tttttctctg ttccacaaga ttttttctct tcagtagaac
      781 atgatttttc agttttagct ttctcttttt tcttttcttt ttcttttggc tctccagcaa
      841 atgcacttga agtgaataaa aatgctgcaa cagccattaa cgaaactaat tttttcatga
      901 tataaggttt aattattaac aaatgagttg tacaaatata taatttattg aataccgtgc
      961 aacatttcag aattatttta atagaatttt aagttatttt actttataaa aaggtttatc
     1021 attcaaccta aaattaaatt taacaggaat tattaattat tttttttcgc ttttcacaaa
     1081 caaattaaac aacagtcctc ccattaaact tccatataat gtactgttca atggttttga
     1141 cgtgatggaa caagttccac tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 natcatactg cgatatcctc cagcacaatg aatataaaat ggttcatttt ttggaaactg
     1321 aaccaaatgt tcgtttaaaa agtctaacgg agcagatggc acatctacaa tatgttcaga
     1381 agcatactca cccggttttc taacatcaaa tacgggcaca tgaagtttca tgttttcttc
     1441 taaaacttcg ggtccaacag ttgtcacagt atcatattcc attcctgctt ttttccatgt
     1501 ttcaaaccca ccttttaaat acccaatggc attatcgtat cccacacggg ctaatcgggt
     1561 gatggtttca ttttcctttc cttccggagt taccaataaa ataggctgtt tataatctaa
     1621 aatcaaagca cctacccagg gagcaaaacc accatccaat ccaataaaaa ttgattttgg
     1681 aatatgccct ttagcaaaat catcttggtg acgaacatct agcactaaag cttccgtttc
     1741 attagctacg gtttcaaaaa ccttaggttc aaaagcttta gcctctttaa taatattctc
     1801 tacattactg taaccttctt tgttcatttg tacattcatt ggaaaataag ccggtggcgg
     1861 taataaacca tccgttactt ccttcacaaa ttcttcttta gtcatgtttg cccgtaaagc
     1921 ataattcgtt gctttctgat caccaatact tcccacggtt tccttactta aatttttacc
     1981 acaagcagaa cctgctccgt gagccgggta aacaattaca tcatccgcta aggtcattat
     2041 ttttgttcgc aaactatcat ataataaacc ggctaattgt tcctgtgtca tatcggctgc
     2101 tttttgagcc aaatctggtc ggcccacgtc gcctaaaaat aaagtgtctc cgctaaaaat
     2161 agcgtaatct ttaccatttt tatcttttag taaataagta gtactttcca tcgtatgacc
     2221 cggtgtatgc aaagttgtga ttgtaatttc acccaatttg aaaacttcac catctttagc
     2281 aatatgagct ttaaaagtag gggttgccgt tggaccaaaa ataattggag cgttcgtttt
     2341 ttcagataaa gtaacatgac cactcacaaa atcggcatga aaatgcgttt caaaaatgta
     2401 ctttattttt gcattatttt gttctgcttt atcaagataa ggttgaactt ctctcaatgg
     2461 atcaatgatt gctacttcac ctttactttc aatgtaataa gctccttgtg ctaaacaacc
     2521 ggtataaatt tgttctattt tcatatcaat tttattaaga aatgtaaaat tacaatacaa
     2581 taaaaatgtg tgctgtaact ttagttacaa atataacgaa aattaaaaaa gctctatcta
     2641 aactgacaga gctttagcat tatattatgt tttatcaata tttataaaag agttgtcgga
     2701 caaacatagt ccgtaattgg aacagtcgtt tttttaattt cactaaatcc accaatcaca
     2761 tctgtgaaat tttcaaatcc gcgttgtttt aaaatggaag cggcaatcat gcttcgataa
     2821 cctccggcac aactcaatac aaacggtttg tcttttggaa tttctgccat tcgattacta
     2881 atttcgttta gaggaatatt gatggcattc tcaacatgtt ctgaaagata ttcacttttc
     2941 tttctcacat caattaaaat gactttttta tgtcccattt gaacggctaa ttcttccgct
     3001 gtaattctgt ttgattgatc aatttccttt cctgatttta cccatgaatc aaaaccacca
     3061 tctaaataac caatcgcatg gtcataacca acacgagata atcgaataat gctttcttct
     3121 tctctccctg gataagtaac taataaaatt tcttgtttta tatctgcaat gagttctcca
     3181 acccacatcg caaaattact ttctaatcca atattgatgc tatttggaat aaatcctttg
     3241 gcaaaatctt cgggacgacg tgtatctaaa attaaagctc tcgtttcatt cgcaactact
     3301 tcaaattcat caggattaaa tgctttttta cctctttcca taactaaatc caaactttca
     3361 tatccttgaa tattcatcaa aacatttttt ggaaaatacc ccggtggatt agttaatcct
     3421 gtcaatagtt cttttacaaa ttcatcttca gtcatatcag gacgcaaagc ataatttgtt
     3481 ttcttttgat tgcccaatgt atcggttgtt tctttgctca tcatttttcc acaagcagaa
     3541 ccggcaccgt gatttggata tacaattaaa tcgtcagaca aaggcataat tttagttcgt
     3601 aacgaatgga acaaatgacg ggctaattta tcttgtgtca aatcagcgac aacatgttga
     3661 gccaaatcag gtcgtcccac atcacctatg aacaaggtgt cgccactaat gattccatgt
     3721 tctttgcccg tttcatcaat cagtaaatag gtcgtacttt ccattgtgtg ccctggagta
     3781 tgcagcactt tcacttgata attacccact ttaaacactt gattatcttc tgctactaaa
     3841 gcttcaaaac cgggtttagc ggttggtcca tagacaataa tagcaccttc ttttttagct
     3901 aaatctaaat gcccggaaac aaaatcagca tgaaaatgag tttcaaaaac atactttatt
     3961 ttagcattgt cttttcgagc tctatcaatg taaggctgaa cttcccgcaa aggatcaaaa
     4021 atggcagctt caccattgtt ttctaaataa tatgctgcat gagcaatgca tccggtataa
     4081 atttgttcta ttttcatgtt ttaaaattta tgtaaaatta aaataagtta acctgaaaaa
     4141 tgatgacatt aatcagttta ttatttacaa atttataact ttacttattt nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcttctaaat aaagaatttt taataagttt
     4321 aatttcgcca taacaggcac atttaagtaa tctaatttta catcatctcc actgtcaaaa
     4381 gcagaacctt gtgtagaata aaccaactcc ggttgaatcg acataaacac aatgtcaatc
     4441 tcagctaatg caccgacatg aaaccctgac aaggctttat tgccatctaa tccggataat
     4501 gtagcactgt tataacctcc ttttaatcca aactgaacta cttgagcttg aaccgtagaa
     4561 aaagaaaata aaacgataat tgcaaaaatt aattttttca tactatttgt tgtttaagat
     4621 tatatcaaat atagtaaatt ataaataatc gtaataaaaa gttgactaat ttttaaataa
     4681 aaagtttaca aacttcaaga aaaattcact ttgaaaaaaa cttaataagc ctgaatactt
     4741 ccgcttttgc gtttcaaggc ataaattaaa tcattaaaaa ttagcgttgt agatagtaca
     4801 ttatacattt tatcggatgc atttcttggc aaatattcat tcatataagt caactcaaat
     4861 tggatagtat ccgtaaacga ataacctaat cctaacgtta aacgatttcg gtcaaaaaaa
     4921 ccttcgccgg tagtagaacc tttggtttta aaaaccaatt cctcagaaac aattccatat
     4981 aacgatcctt tcccaatctg cacatacgaa attgggtaaa ctaatttgat catttctcga
     5041 aaacgataaa cgatatctac agttccttct gcatttccga gtattctgtc ttcaatccgc
     5101 aatctattgg taagcacata atcggttcta ttaatataat aaatgccttg aaaagcaaat
     5161 cgaacttcgg gtgatttttt ttgagaaatt tcaggcacat cacgattata gaaaaaagcc
     5221 gcaaatccgg ataatttcca ttttttatta gcaaaataat gaagccataa acgagcagaa
     5281 atttgtgaat ttttataaaa cggattgttt tcctcttctc cacttgtaaa tccctgagca
     5341 aaatcaaatt ccattgcagt aagttttcct atatttctat taaactgaaa ttcattggta
     5401 aattgatttt ctgtttgcga aaacaaattg aatgaattaa agaagaatat aacaataata
     5461 caaattgttt tagcaagaaa aatatatgaa aatttattaa tcattatgtt gattcattaa
     5521 aaatcaatta attatcttct ttattctgta taaatcaatt ttaataatcg ttcatctctt
     5581 tcataaatat catcataaaa attaatttcg ccatttgcat tcacccatgc cgtaaaataa
     5641 ccgatgtaaa ccggtatttt attttttaac gtataccaat tctctttccc tgaattcatg
     5701 gctgcatcta ttttttccgt tgtccaattt ggatcatctt ttaaaatggt gagggctaaa
     5761 tctcttgctt ttcctacacg aatacaacca tgactgaatg ctctcgtttc tttttcaaat
     5821 aaacttttag caggggtatc atgtaaatag atattattgg aatttggaaa aataaattta
     5881 actaaaccta acgaattatt tttaccgggt ttttgtcgaa tgttaccatt attccactcc
     5941 atattatggg aagccaaata atttttgttt ttggccatag ccggtttaat ttcttttttg
     6001 ataatacttg ttggcacatt ccaataggga ctaaaaacaa tataactcat tgttccactg
     6061 aaaattacgg ttttattcat agccttacca acaacgacag gcgaaactaa ttcctgtttt
     6121 ccatttctga caaaaaataa tttataagag ggaatattaa ccaccacata ttcatttgct
     6181 ttgaccattt c
//
LOCUS       DAOTCW010000132         6171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_9358_length_6171_cov_50.280085, whole genome shotgun sequence.
ACCESSION   DAOTCW010000132 DAOTCW010000000
VERSION     DAOTCW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9358_length_6171_cov_50.280085"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="P5335_09635"
     CDS             complement(<1..815)
                     /locus_tag="P5335_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRZ75181.1"
                     /translation="MSKKPKKLGNKNKDFTTQIFKILSKETNKSFNYKQIAAILELDD
                     TKSRNEIIRDLKILKAQDKIHEIEIGKYQIISKAEYYEGTIDMTSRKTGYFVCDELEE
                     DVFVPFINLNHALDNDKVKAYIYNRRSSRRPEAEVLEILERAKTEFVGVIDVQKNFAF
                     VTTANAKMYTDIFIPKNKMGDAEHGDVVLVKMEDWPAKADSPFGSVIKVLGKPGEHNT
                     EIHAILAEYGLPYDFPIEVEAYANKIDTSITKEEIAKRRDMRKTLTFTIDPRDA"
     gene            complement(899..2458)
                     /locus_tag="P5335_09640"
     CDS             complement(899..2458)
                     /locus_tag="P5335_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ75182.1"
                     /translation="MNKLLKKLLFVGSIVYVGISTIQAQAPEIQWQKSIGGTGTDIAY
                     SIIQTSDNGFIVAGNSSSNNGDVSGNHGLGDYLVVKLSVTGNIEWQKSLGGTNEDDAR
                     SIQQTLDGGYIVVGSSNSNNGDVSGNHGGADYWIVKLNSIGDIQWQKSLGGLSNEGAN
                     SIKETEDGGYIIAGTSASTNSDVSGNHGSIDYWIVKINIEGNIEWQKSLGGSNQDNLC
                     SITQSFDGGYVIVGYSVSNDGDVSGNHGGVDYWIVKINSIGTIEWQKSLGGSNDDIPF
                     SITQTIDGGYIIAGNSSSNDGDVSGNHGYVDCWIVKINSIGIIEWQKSLGGSNQDFVY
                     SIIQISDGSYIIAGNSSSNDGDVSGNHGSLDCWMVKINSSGTIIWQKSLGGTGNDIPF
                     SIIQTIDGGYIIAGYSDSNNGDVTGNQGYEDFLIIKLSQDNLSIDSFSNNNAISLFPN
                     PTKESLSIKLDYYTPLQEIIITDVLGKIIHSQKVDGLITTIITDGFKNGVYFVNTMDG
                     AKMFTQRFIKI"
     gene            2574..2810
                     /locus_tag="P5335_09645"
     CDS             2574..2810
                     /locus_tag="P5335_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRZ75183.1"
                     /translation="MQNFITVAIFNLPTEIAVLKSILENEGIQYFFENETIVSIDPFA
                     SIAYGGIKLKVHQNDVQVVQRILDNLDSHLRIVE"
     gene            complement(3399..4580)
                     /locus_tag="P5335_09650"
     CDS             complement(3399..4580)
                     /locus_tag="P5335_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HRZ75184.1"
                     /translation="MKKYYYIILGSGLSGLLTAYRMANDAWFDDKEILIIDKEIKNQN
                     DRTWCFWEESNGEFDSILSKTWEKAFIGNQDFQQSFEMKPYLYKMIRSRDFYKLVFGT
                     ISSKSNFTFENDEIINWKTIEDSVEITGKNQSYFGLFLLNSFFDVKPILNQKKYPYLK
                     QHFIGWFIKTKEAIFDDSEVKFMDFTIKQDGNTRFMYVLPTSKTEALFEYTLFSEDLL
                     EKTEYENAISNYLQNIGITNYEIMEKETGNIPMTCFPFHNQNSERILFIGTAGGWTKA
                     STGFTFFNSKKQSEKLVEFLKTNKNLSKFHQKNRYWFYDLILLEVLHQNNELGGQLFG
                     TLFQKNNIQNIFQFLNEEGTIFSDLKVMLTLPKRLFIKATFRAFWKLLKKKNEFLSFN
                     H"
     gene            complement(4674..5192)
                     /locus_tag="P5335_09655"
     CDS             complement(4674..5192)
                     /locus_tag="P5335_09655"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HRZ75185.1"
                     /translation="MKFNGQVCSFDDNALYWELHIPIPDAVFNKLYLEAKDKRVICTI
                     NNDYSFHCAMLPKVTFHYILLNKEICKKLNLHLNDDVKVELVKDESKYGMPLSEEMEE
                     VFFSDPEGSDLFHQLTPGKQRTLIHVVNKFKSSQLRIEKSFVILKHLKNRKGVLDFKV
                     LNEDFKDYKNKL"
     gene            5251..5682
                     /gene="rpiB"
                     /locus_tag="P5335_09660"
     CDS             5251..5682
                     /gene="rpiB"
                     /locus_tag="P5335_09660"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRZ75186.1"
                     /translation="MKISIGNDHAGPDYKKAIVDFLEKQGHEIINHGTNSTDSVDYPD
                     FGHPVATDVETGKAELGIVICGSGNGIAMTVNKHQDIRAALCWTKEIAALARQHNDAN
                     IISIPARFTSIPQAVEMVETFLKTEFEGGRHQNRVNKISCS"
     gene            5772..>6171
                     /locus_tag="P5335_09665"
     CDS             5772..>6171
                     /locus_tag="P5335_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRZ75187.1"
                     /translation="MSTNPRIHKHNVKGNNLIFSILLNTIITIAQVIGGIVSGSLALI
                     SDALHNFSDVLSLIFSLVAHKLSRRKASVNQTFGFKRAELIAAFINAATLIIVAFILI
                     YHAIIKLMHPEPIESDLVIWLALLGIAVNGF"
BASE COUNT         2102 a         1028 c          817 g         2224 t
ORIGIN      
        1 gcatctcttg gatcaatagt aaacgttaag gttttacgca tatcgcgacg tttagcaatt
       61 tcttctttcg taatagatgt atctatttta ttggcataag cttctacttc aataggaaaa
      121 tcatacggca aaccatattc cgcaagaata gcatgaattt ctgtattgtg ttctcctggt
      181 tttcctaaaa ctttaataac agaaccaaat ggactatccg cttttgcggg ccaatcttcc
      241 atttttacta acacaacatc accatgttcg gcatcaccca ttttattttt aggaatgaat
      301 atgtcggtgt acatttttgc attggctgta gttacaaaag caaaattctt ttgaacatca
      361 attaccccta caaattctgt tttagctctt tctaaaattt ctaaaacttc agcttccggt
      421 cggcgggaac ttcttctgtt gtagatataa gcttttactt tatcgttatc caacgcatga
      481 tttaaattga taaaaggtac aaatacatct tcttctaatt catcacaaac aaaatagcct
      541 gtttttcgag aagtcatgtc gatggttcct tcataatact ctgctttgga gataatttga
      601 tatttaccaa tttctatttc atgaatttta tcctgagctt ttaatatttt taaatcacgg
      661 ataatttcgt ttctgctttt tgtatcatca agttctaaaa tagcagctat ttgtttataa
      721 ttgaatgatt tattggtttc tttagacaga attttaaata tctgtgttgt aaaatcttta
      781 ttcttgtttc ctaatttttt tggtttctta ctcataattc ttttctaata aagttgaaaa
      841 ttacgaataa gatgggagaa aaattaaatt agctaaaaga tttagctaaa aaataacgtt
      901 atatttttat aaatcgttgt gtaaacattt ttgctccatc catagtattt acaaaataaa
      961 ccccattttt aaatccatca gttataatag ttgtaattaa cccatctact ttttgagaat
     1021 gaattatttt tcctagaaca tctgtaataa taatttcttg taaaggagta taataatcaa
     1081 gtttaataga aagactttcc tttgttggat ttggaaaaag tgaaatagca ttattgtttg
     1141 aaaatgaatc tattgataaa ttatcttgac taagttttat aatcaaaaaa tcttcatatc
     1201 cttgatttcc tgttacatct ccgttgtttg aatctgaata tcctgctata atataacctc
     1261 catctatagt ttgtatgata ctaaaaggaa tatcattccc tgttcctcca agtgattttt
     1321 gccaaattat agttccactt gagtttattt ttaccatcca acaatcaaga cttccatgat
     1381 ttcctgatac atctccatcg tttgaagatg aatttcctgc tataatataa ctcccatccg
     1441 aaatctgaat tatactgtaa acaaaatctt gattagaacc tcctaaactt ttttgccatt
     1501 caattattcc tattgaattt attttcacta tccaacaatc aacatatcca tgatttcctg
     1561 atacatctcc atcgtttgaa gatgaatttc ctgctataat ataacctcca tctatagttt
     1621 gtgtaatact aaaagggata tcatcgttag aacctccaag tgatttttgc cattctatag
     1681 ttcctataga atttattttc actatccaat aatcaacacc tccatgattt ccggaaacat
     1741 ctccatcatt tgaaactgaa tagccaacta taacataacc accatcaaaa gattgtgtaa
     1801 tactacataa attatcttga ttagaacctc caagtgattt ttgccattca atatttcctt
     1861 ctatattaat tttcactatc caataatcaa tacttccatg atttccggaa acatcactat
     1921 ttgttgaagc tgaagttcca gcaataatat atccaccatc ttcagtttct ttaatactat
     1981 tagcaccttc gttacttaat ccacctaatg atttttgcca ctgaatatct ccaatagagt
     2041 ttaatttaac tatccaataa tcagcacctc catgatttcc tgatacatct ccattatttg
     2101 aatttgaaga acctactaca atataacctc catctagagt ctgctgaata cttcgtgcat
     2161 catcttcatt tgttccacct aaagactttt gccattcaat attccctgtt actgataatt
     2221 taactactaa ataatctcct agaccatgat ttccggaaac atctccattg tttgaacttg
     2281 aatttcctgc tacaataaat ccattatctg aagtttgaat aatagaatat gctatatccg
     2341 tgccagttcc accaatagac ttttgccatt gaatttcagg tgcttgagct tgtattgtac
     2401 taattccaac ataaactata cttccaacaa aaagtaattt ttttaataat ttattcatta
     2461 aaataataat tagtatgtgt gtaaaagtat aaaaaacact ttcacattac tctctatata
     2521 tgtttaaaaa ttaattaaaa ataacattta ctatctttat aaaaaaagtt actatgcaaa
     2581 attttataac tgttgcaatt tttaatttac ccacagaaat tgcggtttta aaatctattt
     2641 tagaaaacga aggaattcaa tacttttttg aaaatgaaac cattgtttca atagatccgt
     2701 ttgcgagcat cgcatacggt ggtattaaat taaaagtaca tcaaaatgat gtgcaagttg
     2761 ttcaacgtat tttagataat ttagacagtc atttacgaat agtagaataa tttgatatgt
     2821 cttaaaaaaa tctttatttt aaaaatttaa gagagttttc aacacttatt aaaaacaaca
     2881 aaaagaaaaa gaattttaaa tttaaattta tgatggttat tatagtgtgt tcatttgtac
     2941 aataactttt taagttaaac ttgattttaa aagttgtggt aagttataca gacttgttaa
     3001 catagaaaaa taataataaa gacctcaaaa tgaataattt tttagttttt attaacagtt
     3061 gttaacatca aaatttttga tgaaattatt aataagtttg ttataaaaaa ctgttgaaaa
     3121 cagagttaaa taagttcata attttaaagt aaaaaatcaa aacaaaagtt gatttattca
     3181 ttcctctttt tgattagaaa aaatttccat acatcatcaa aatacttcta aaattctatc
     3241 tgaatttatc aacaactcat gttaatttaa tgttacctca gtatcaagcg attatgaaat
     3301 actgttaaca atcaaaatat ttaacgttta ctttatgata aagataagat aatcaatgat
     3361 ttaatgaaat agtatatgtc ttaataaaac ttaaattctt aatggttaaa agagagaaac
     3421 tcattttttt tctttaacaa cttccaaaaa gcacgaaaag ttgctttaat aaataacctt
     3481 ttaggaagtg tcagcatcac ttttaaatca gagaaaatag ttccttcttc atttaaaaat
     3541 tgaaagatat tttgaatgtt atttttttga aaaagtgttc caaaaagttg ccctcctaat
     3601 tcattatttt gatgtaaaac ttccaacaga attaagtcgt aaaaccaata gcgatttttt
     3661 tgatgaaatt tggagaggtt cttattagtt tttaaaaatt ccaccaattt ttccgattgt
     3721 tttttactat tgaaaaaagt aaaacctgtg cttgctttag tccaaccacc ggctgttcca
     3781 atgaataaaa tccgttcaga attttgatta tgaaacggga aacacgtcat cggaatattt
     3841 ccggtttctt tttccataat ttcataattg gtaattccta tattttgaag ataattagaa
     3901 attgcgtttt cgtattccgt tttttctaat aaatcttccg aaaacaacgt atattcaaac
     3961 aaagcttcgg ttttggaagt tggtaaaaca tacataaagc gagtatttcc gtcttgttta
     4021 atcgtaaaat ccataaattt tacttcagaa tcatcaaaaa ttgcttcttt ggttttgatg
     4081 aaccaaccga tgaaatgttg ttttaaataa ggatactttt tttggttgag aatgggttta
     4141 acatcaaaaa agctattcaa taaaaaaaga ccaaaatacg attgattttt tccggtgatt
     4201 tcaactgaat cttcaatcgt tttccaatta attatttcat cattttcaaa agtaaaattt
     4261 gatttggatg aaattgtacc gaataccaat ttataaaaat ccctgcttcg aatcatttta
     4321 tacaaatacg gtttcatctc aaatgattgt tgaaaatctt gattcccgat gaatgctttt
     4381 tcccaagttt tagaaaggat agaatcaaat tcgccattcg attcttccca aaaacaccag
     4441 gttcggtcgt tttggttttt gatttcctta tcgatgataa gaatttcttt gtcatcaaac
     4501 caagcatcat tcgccatacg ataagcggtg agcaaacctg ataaaccgga accgaggatg
     4561 atgtaatagt attttttcat tatctaaacc gcaaattcgc aaattttgta taaaagtaaa
     4621 gattttgtat cgcatccatt aattaaaatt ttaattagcg aatttgcggt ttattacaat
     4681 ttatttttat aatccttaaa atcctcattc aacaccttaa aatccaaaac accttttctg
     4741 tttttcaaat gttttaaaat aacaaacgat ttttcaatcc gcaattgtga acttttgaac
     4801 ttatttacca catgaatcaa cgttcgttgt tttccgggag ttagttggtg aaataaatca
     4861 cttccttcgg ggtcgctgaa aaaaacttct tccatttctt ccgaaagagg catgccgtat
     4921 ttgctttcgt ctttcactaa ttcaactttt acatcgtcat tcaagtgcaa attcagtttt
     4981 ttacagattt ccttatttaa taaaatataa tgaaaagtca cttttggcaa catggcacaa
     5041 tgaaaagaat aatcattatt tattgtgcaa atgactcgtt tatctttagc ttctaagtat
     5101 aatttattaa aaacagcatc aggaattgga atatgtaatt cccaatacaa agcattgtca
     5161 tcaaacgaac aaacctgacc gttaaatttc atcctaacct gattgaattt gattattttt
     5221 gtaaagataa aaataacaac acaacaacac atgaaaatat ccattggaaa cgatcacgcc
     5281 ggcccggatt ataaaaaagc catcgttgat tttttagaaa aacaaggtca cgaaatcatt
     5341 aatcacggaa ctaactcgac cgatagtgtg gattatcccg attttggtca tccggttgca
     5401 accgatgtag aaaccggaaa agccgagtta ggaatcgtca tatgtggtag cggcaacgga
     5461 attgcgatga ccgtaaacaa acaccaagat attcgagcag ctttatgttg gaccaaagag
     5521 attgcagctt tggccagaca acataatgat gctaatatta ttagtattcc ggcacgattt
     5581 acttcgattc cgcaagcggt tgaaatggtg gaaacttttt tgaaaacgga atttgaaggc
     5641 ggaagacacc agaatcgagt caataaaata agttgctcat aacagttgaa tcattaagga
     5701 attaagcgac attaaaaaat taaaacttaa tgaaccttga ttccttaatg gttttaaaaa
     5761 caaaaactta aatgtccacc aacccaagaa ttcacaaaca taacgtaaaa ggaaataatt
     5821 taattttttc cattttactc aatactataa ttaccattgc tcaagttatt ggtggaattg
     5881 tttccggaag tttagcttta atttctgatg cgttgcacaa tttttcggat gtactttcgc
     5941 tgatttttag tttggtggcc cacaaattat cccgaagaaa agcctctgta aatcaaactt
     6001 ttggttttaa aagagcggaa ttgattgctg cttttatcaa cgcagcaacg ttaattattg
     6061 tcgcttttat attgatttat catgcgatta ttaaattaat gcatcccgaa ccaattgaat
     6121 cggacttagt catttggtta gcccttttag gaatcgccgt caatggtttt t
//
LOCUS       DAOTCW010000133         6132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_9459_length_6132_cov_37.549449, whole genome shotgun sequence.
ACCESSION   DAOTCW010000133 DAOTCW010000000
VERSION     DAOTCW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9459_length_6132_cov_37.549449"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="P5335_09670"
     CDS             complement(<1..1277)
                     /locus_tag="P5335_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HRZ75188.1"
                     /translation="MPLFQNTVISKYLKTQDKNLLQEKWLQFQNHFHNPTIQENIRNS
                     KEEQYQGEFLLDLFVAILGYTKNPQPDFNLTTEYKNIKDSKKADGAIIINDKVVAVIE
                     LKGTNTTDLSKIESQAFGYKNNQQDCNYIITSNFEKLRFYIDNAIEHIEFNLFKLTKS
                     EFELLYLLLGCKNIAKDFAKNIKEESLSQEEAITKSLYKDYSNFKRELHQNLVTKNTN
                     YDPLELFKKSQKLLDRFLFLFFAEDRHLLPPNSVRLVLSDWKDLKDRDVEIPLYDRFK
                     KYFEYLNTGYKGKRYDVYAYNGGLFKPDDILDHITIDDDLLYKHALKLSDYDFASEVD
                     VNILGHIFENSLNELDEIKAQLEGLEIDKSKTKRKKDGVFYTPKYITKYIVENTIGKL
                     CKEKKDYLQIIENDYIIDKKRQKNTIKLLQNKL"
     gene            complement(1809..3032)
                     /locus_tag="P5335_09675"
     CDS             complement(1809..3032)
                     /locus_tag="P5335_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013868956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HRZ75189.1"
                     /translation="MKELDKYIRVGTEFYKIIKSSSGNKVLQKWNRQTLIDDYGKDSI
                     AKVNKYESFCIVPSHNKYEQIINNCYNQYQLLSYELKEGNKYQNIIHFLQHIFGEQFE
                     IGLDFLTILWQKPTQILPILCLVSTERNTGKTTFINLLKLIFEYNMTINTNDDFRSRF
                     NADWSGKLIIAVDEVLLDKREDSERIKNLSTAKSNKIELKGKDKFEQDFFGKFILCSN
                     NEDSFIKIDSEEIRYWIRKINPLQNHDDINLIKKMKEEVPCFANFLNTRNISTIPTTR
                     MWFSAEQLYTPSLGKLINGNRSYVEKELEELLIDEFENSCEDELCYTAKNLVDMLKRN
                     NVLAQSNYIVKLLKEKYKLKPVNSSYKYYRTEFKSPENINGINFTTEKGRYYTFKREN
                     FVFAVEVLKTAHNFQ"
     gene            complement(3029..3352)
                     /locus_tag="P5335_09680"
     CDS             complement(3029..3352)
                     /locus_tag="P5335_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75190.1"
                     /translation="METIQTKLESRRNDILYYLDKTIDKIKLLQLENIRNKEIYLVSD
                     SAISDISITKQLIETSNLLLPIEIANNVNQIFIADKTVSSVIVNIVNSKRTIKDCPAY
                     LKLTV"
     gene            complement(3454..3741)
                     /locus_tag="P5335_09685"
     CDS             complement(3454..3741)
                     /locus_tag="P5335_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRZ75191.1"
                     /translation="MTKQILQIENTNTTDFKKEILDGFSEVLKDFANNLQSKENDVLL
                     TREETAKLLSVSLVTLWDWTKKDILPAYRIGNKIRYKKSEVLNSMSKRNQF"
     gene            complement(3806..4657)
                     /locus_tag="P5335_09690"
     CDS             complement(3806..4657)
                     /locus_tag="P5335_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75192.1"
                     /translation="MKYSEHYNQHYDKIYSLVEFKIKKYFIDCKSKKLNYGICYNDLL
                     VIINKYLKNRISEKNTDIKALHTIFNNRLLRYSEDEIDLIIENDILIEFQKLNNIELP
                     KTNSIYKLINEFALRDVLKEITRLLQNNSRLFQLFYELNEFDQFEIKYYRLQRLDDTE
                     IFINLNKRKNPHLYTEPNTTFFNNETANELSLKNPSLPLAITILNEIGFFELEKLKSF
                     TDNQLSQLIAIIQIKDPNNKNELRSIGGNIRMVKGSNDNPSRYTAGSNKNESLAKSLL
                     TEIKLGL"
     gene            complement(4665..5924)
                     /locus_tag="P5335_09695"
     CDS             complement(4665..5924)
                     /locus_tag="P5335_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRZ75193.1"
                     /translation="MNLKFFIVEGKKKYTSIHVRFWDSKRIDQKTKTGITVSADDWSS
                     SKQRLKPKATTTNRDFLNHQLEQVERYVIDKYNLDYNSKKFISEKWLKETIENFFGRV
                     TEDNNYKIYFVDWVELFVKEAPKRMYNGKPIAERTIKNYTTTLNKLKAFEKVQNHKYR
                     FEDIDLNFHRDFIHYSSTVEKLNNNSIGSLITRIKTFCSNIELDGYSINPKYKHRDFK
                     APSNETKDIYLNEEEINKIAKHDFSDKESLDNARDLFIIGLRTGLRVSDFLRIGKENV
                     IDNIINITTQKTNQNLYIPIHPQFQEVLNKRNGEFPREISDQKFNLYIKTACEEVGLK
                     EMVFGAKINPETNRKEFKIYPKHELVTSHICRRSFATNLFLAGFDNSTIMKATGHQSE
                     AQFLKYIKASQTEHLQKISAYWENNKK"
BASE COUNT         1854 a          873 c          888 g         2517 t
ORIGIN      
        1 tccagtttgt tttgcaaaag ttttatggtg tttttttgtc tttttttgtc aatgatatag
       61 tcgttttcta taatttgcaa ataatctttc ttttctttgc aaagtttgcc aatagtattt
      121 tcaacgatat atttggtaat gtattttgga gtgtaaaaaa caccatcttt tttgcgtttg
      181 gttttcgatt tgtcaatttc tagcccttcc aattgggctt ttatttcatc caattcattt
      241 aaggagtttt caaatatatg ccccaaaatg ttcacatcca cttcacttgc aaaatcgtaa
      301 tcagataatt ttaaagcatg tttgtataac aagtcatcat caatagttat atggtctaat
      361 atgtcatcag gcttaaataa accgccgtta taagcataga catcataacg ttttccctta
      421 taaccagtat ttaagtattc aaagtatttc ttgaatcggt catataatgg aatctcaaca
      481 tctctatctt tcaaatcttt ccagtcactt aaaaccaaac gtacagaatt tggcgggagt
      541 aagtgcctgt cttcagcaaa aaacagaaat agaaaacgat caagtagctt ctgcgatttt
      601 ttaaaaagtt ctaaggggtc ataattagta ttttttgtaa ctagattttg atgcaattcc
      661 cttttgaaat tagagtaatc tttatataaa ctctttgtta tagcctcttc ttggcttaat
      721 gattcctctt taatgttttt tgcaaaatct tttgcaatgt ttttgcaacc caataaaagg
      781 tataataatt caaattcact tttagttaat ttgaaaagat taaactcaat atgttcaata
      841 gcattatcaa tataaaagcg taatttttca aagttagatg tgataatgta attgcaatcc
      901 tgttgattat ttttataacc aaaggcttga gattcaattt tgcttaagtc agtcgtgttt
      961 gtacctttaa gttctattac agcaactact ttatcattta taattattgc tccatccgct
     1021 tttttgctgt ctttgatgtt tttgtattcg gttgttaaat taaaatcagg ttgcggattt
     1081 ttggtgtaac ctaaaatggc aacaaacaag tcaagtaaaa attcaccttg atattgttct
     1141 tctttactat ttctgatatt ttcctggata gttgggttat gaaagtgatt ttggaattgc
     1201 aaccactttt cttgcaaaag atttttgtct tgtgttttta ggtattttga aattacggta
     1261 ttttgaaata aaggcattga aaatcattga ttgattagat tttataaatt tagtaagaaa
     1321 atgtgattta ttttttattt tctaataatc tgttttcaac aattttagtt ttttatctcg
     1381 tgttaaaagt atttcttttt tgatgctgat tatatgatat tttaaattcc ccgattaaaa
     1441 gttgataata taattttgta tgcaaaaagc atattatttg tagtaaaatt acaccggaat
     1501 actcagaatt ccggaaaatc gttccgggaa cgttcggaaa tggatcgggg aacgttccgg
     1561 gaacgtatcg gtaaaattcc gtgaacgatc ggaaatcatt ccgggaaatt tccgggaaca
     1621 ttctcacaag aagggcatag gttcgtgcat gattttgaaa ataattaaat gtaaatggaa
     1681 aaaactgttg aactgttgaa ttttgaaaaa agacttgaat tcattcagat tatgattcaa
     1741 cagtagtttt tttatttcaa cagcttgttg aatattcttt tgaaagtgtt gaatgaataa
     1801 tgcttatttt attgaaagtt gtgtgcagtt ttcaacactt caacagcaaa gacaaagttt
     1861 tctcttttaa aagtataata acgacctttt tcagtcgtga aatttattcc atttatattt
     1921 tctggacttt taaattcagt tcgataatat ttataactac tgtttacagg ttttaatttg
     1981 tacttttctt tcagtagctt aactatgtaa ttactttgag caagtacatt atttcttttc
     2041 agcatatcca caaggttttt tgcagtatag cataattcgt cttcacagga attttcaaat
     2101 tcgtcaatca aaagttcttc caattctttt tctacataac tacgatttcc attgattaat
     2161 tttcctaaag aaggagtgta aagttgttca gcactaaacc acattcgagt agtgggaatg
     2221 gtgcttatat ttctggtgtt tagaaaattt gcaaaacagg gaacttcctc tttcattttt
     2281 tttattaagt taatatcatc atggttttgc aatggattaa tttttctaat ccaatacctt
     2341 atttcttcag aatcaatttt tataaaactg tcttcattat tagagcaaag aataaatttt
     2401 ccaaaaaagt cttgttcaaa tttatctttt ccttttagtt caattttatt tgactttgct
     2461 gtagataaat ttttgattct ttcgctgtct tctcttttgt ccaaaagaac ttcatctact
     2521 gcaataatta atttaccaga ccaatcagca ttaaatctac ttctaaaatc atcatttgta
     2581 ttgatggtca tgttgtattc aaaaatcaac tttaataaat tgataaatgt tgtttttccg
     2641 gtgtttcgtt cggtgcttac caaacataga attggcaata tttgtgttgg cttttgccat
     2701 aaaattgtga gaaagtctaa cccaatttca aattgctctc caaagatatg ttgcagaaag
     2761 tgaataatat tttgatattt atttccttct tttaattcat agcttaaaag ttggtattgg
     2821 ttgtaacaat tattgataat ttgttcatat ttattgtggg aaggaacaat gcaaaacgat
     2881 tcgtatttat ttacttttgc aatggaatct ttaccatagt catcaataag agtttgccgg
     2941 ttccattttt gcaaaacttt gttaccactt gatgatttta taatcttata gaactctgtg
     3001 cctacacgga tatatttatc tagctctttc atacggtaag ttttagatag gcaggacaat
     3061 ccttgattgt tctttttgag ttaacaatat tcacaataac tgagcttaca gttttgtcag
     3121 caataaaaat ttgattcaca ttgtttgcaa tttcaatagg taatagcaaa tttgaagttt
     3181 caattaattg ctttgttatg ctaatgtctg aaatagctga atcagaaacc aaataaattt
     3241 ctttatttct aatgttttcg agttgcaata attttatttt gtcaatcgtt ttgtctaaat
     3301 aatacagaat atcatttctt ctggattcaa gttttgtttg tatggtttcc ataattcttg
     3361 ttgatttaaa ttaattttaa aatcccattg cattttaaac aacctaacta tacaatttgt
     3421 aattagttat aatttaaaat gtgagcagaa ttgttagaat tgatttcttt tactcatgga
     3481 gttaagtacc tcgctttttt tgtacctgat tttgttcccg attcggtatg ccggtaaaat
     3541 gtcttttttt gtccaatccc acaaggtaac aagggacacg gatagaagtt tggctgtttc
     3601 ctctctagtg aggagaacat cattttcttt ggattgcaaa ttatttgcaa aatcctttaa
     3661 gacttcacta aatccatcta gaatttcttt tttgaaatct gttgtgttag tgttttcgat
     3721 ttgaagaatt tgttttgtca ttactctatt ttttaattaa taatagagct aaattaagcg
     3781 tgcttattct gaggactagt cctatttaca gtcctagctt aatttcagtt aataatgatt
     3841 ttgctaaact ttcattttta tttgagccag cagtatatct agatgggtta tcattagacc
     3901 ctttaaccat tctgatattt cctccaatag agcgtaactc atttttatta ttagggtctt
     3961 taatttgtat tattgcaata agttgtgaaa gttggttgtc tgtaaaagat tttaattttt
     4021 ccaattcgaa aaaccctatt tcatttaaaa ttgttatggc caagggtaaa gatggatttt
     4081 ttaaactaag ttcattggct gtctcattat taaaaaatgt tgtgtttggc tctgtatata
     4141 aatgcggatt ttttctttta tttaaattaa tgaatatttc cgtatcatcc aatctttgta
     4201 gtctgtaata tttaatttca aattgatcaa attcgtttaa ttcataaaat aattgaaaaa
     4261 gtcttgaatt gttttgcaat aatctggtga tttcttttaa tacatcccta agagcaaatt
     4321 catttattaa tttataaatc gaattggttt tgggaagttc aatgttattt aatttttgaa
     4381 attctattaa aatgtcattt tcaataatta aatcaatttc atcttcagaa tacctcaata
     4441 gtctattatt aaaaatagta tgtagagctt tgatgtctgt gtttttttct gaaattctgt
     4501 tttttaaata tttatttatt atcactaata aatcattata gcaaatacca taatttagtt
     4561 ttttactttt acagtcgata aaatattttt taatcttaaa ctcaaccaat gaatagattt
     4621 tatcgtaatg ttgattgtaa tgttctgaat atttcataat gttattactt tttgttgttc
     4681 tcccaatacg cactaatttt ttgcaaatgt tcagtttgtg aggccttaat gtatttcaaa
     4741 aattgtgcct ctgattgatg tccggttgct ttcataatag tagaattgtc aaaaccagct
     4801 aaaaataaat tggttgcaaa cgaccttcta caaatatgtg aagttaccaa ttcatgttta
     4861 gggtagattt tgaattcttt acgattggtt tcaggattta tttttgcacc aaaaaccatt
     4921 tcttttaaac ctacttcttc acaagctgtt ttgatataaa gattgaattt ttgatcggat
     4981 atttcccttg gaaattctcc gtttcttttg tttaaaactt cctgaaactg tggatggatt
     5041 ggaatatata aattctggtt tgttttttgg gtagttatat taattatatt atcaattaca
     5101 ttttctttac caattcttag gaagtcagaa accctcaatc ctgttcgcaa tccaatgata
     5161 aacaaatccc ttgcgttgtc tagtgattcc ttatcgctaa aatcatgttt tgcaatttta
     5221 tttatttctt cttcatttaa ataaatatct ttcgtttcat tagacggagc tttaaaatca
     5281 cggtgtttgt attttggatt gatagaataa ccgtctaatt caatgttgga gcaaaaagtt
     5341 tttattctag ttattagaga tccaatagaa ttattattta acttttcaac tgttgagctg
     5401 taatgaataa aatctctgtg aaagttcaaa tcaatatctt caaatcgata tttgtggttt
     5461 tgaacttttt caaacgcttt taatttattg agggtcgttg tatagttttt aattgttcgc
     5521 tctgcaatcg gtttaccatt atacattctt ttgggagctt cttttacaaa tagctcaacc
     5581 caatccacaa aataaatttt ataattattg tcttcagtta cccttccaaa aaagttttct
     5641 atagtttctt ttaaccattt ttctgaaatg aattttttgg aattataatc cagattatat
     5701 ttatcaatta cgtatctttc tacctgttct aattgatggt ttaaaaagtc tctgttggtt
     5761 gttgttgctt tgggctttaa tctttgtttg gatgaagacc aatcgtcagc tgatacagta
     5821 atacctgttt ttgttttttg gtcaattcgt ttactgtccc aaaaacggac atggatacta
     5881 gtgtactttt tctttccttc aactataaaa aatttcaaat tcatatacta tttttgccga
     5941 atatttgccg aaaagtagtt aatttttttg aatttgactt aatttaactt aatatttttt
     6001 ttgcacacat ttttaaaaac tacttaaata taaaggtttt caaaggtttt gagtggtttt
     6061 ttcaatttga gaagattaag aattgcgact tccactccgg gtacaaaacc tctcagaaat
     6121 gggaggtttt tt
//
LOCUS       DAOTCW010000134         6090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_9552_length_6090_cov_32.991549, whole genome shotgun sequence.
ACCESSION   DAOTCW010000134 DAOTCW010000000
VERSION     DAOTCW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9552_length_6090_cov_32.991549"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="P5335_09700"
     CDS             complement(<1..664)
                     /locus_tag="P5335_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HRZ75194.1"
                     /translation="MKFLETEYEKKSFALSASITIGLLLLFFVFGLTYMDPPPENGIA
                     INFGNTDAGSGNTNTTELVKSELREVTEQPKVEETSEPTTPSKASENVLTADNDAAIA
                     LKKQKEIADAKASAEAKIKAAEQKKKQEEADKKAKLDALIGGVKKSDGKTSGGDGTTT
                     NSGNQGKIDGSIYSNSYYGSGKGTGTGNGSWGLNGRKLSKPGAVKADCNDEGTVVVEV
                     KVS"
     gene            complement(673..1065)
                     /locus_tag="P5335_09705"
     CDS             complement(673..1065)
                     /locus_tag="P5335_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRZ75195.1"
                     /translation="MNIRGRNKVSPEFNMSSMTDIVFLFLIFFMLTSTMVTTNALDLV
                     LPKAKGKTDSNKNISVSINKDLEFFIDKDQVQEADLESKLLALFSNKDDKAIILRAEE
                     GVPIEKAVNVLDIANRNQIKVVLAVRPK"
     gene            complement(1067..1762)
                     /locus_tag="P5335_09710"
     CDS             complement(1067..1762)
                     /locus_tag="P5335_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRZ75196.1"
                     /translation="MQAIDSLATTQNAVLDPQPIEKTLSIWELVSSGGIGGQMIMITL
                     GLLFFFALYLYFERLMAINAASKIDVNFMNNIKMNIMNGKIDAAKMLCAQTNSPVARL
                     TEKGISRIGKPLEDINTAIENAGKLEIYKLEKNVSMLATISGAGPMIGFLGTVIGMIL
                     AFHKMASGGGQIEVGALAEGIYTAMTTTVAGLVVGIISFMGYNHLVVKTDKVVHQMEA
                     NATDFLDLLNEPS"
     gene            complement(1810..3183)
                     /gene="nhaD"
                     /locus_tag="P5335_09715"
     CDS             complement(1810..3183)
                     /gene="nhaD"
                     /locus_tag="P5335_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009401215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HRZ75197.1"
                     /translation="MALFIILLFVVGYLSITLEHPLKLDKTVPALLMASVMWAFLAIG
                     FHQGWFTVIDGYGSVFNIASGEIHQQEHGFESLLLHHLGKTAEIIVFLIGAMTIVELI
                     DLHRGFDVLKGMVKTTSKIKLLWITGIIGFFLSSVIDNLTATIVLISLLRKLIQNREE
                     RIWYVSLIIIAANAGGAWSPIGDVTTTMLWIAKKVSAVGLSEYIIVPAVICFVVPFAL
                     ASRMKIFKGDIQIEIKNNEEEQKLLSSKTMLWVGLGAIVFVPIFKTLTHLPPYVGMMF
                     SLAIVWLVSEYIHPEDHFDKSKKHLYSAQKALSRIEISSILFFLGILMAVAALESLVF
                     GTIQGQEVGTLRYAAESISNAIPNMDVVIILLGILSAVIDNVPLVAASMGMYTYEIDH
                     PAWHFIAYSAGTGGSMLIIGSAAGVAAMGMEKIDFIWYLKKITWLAFAGFIAGAIAFL
                     FIEHFVR"
     gene            complement(3194..4420)
                     /locus_tag="P5335_09720"
     CDS             complement(3194..4420)
                     /locus_tag="P5335_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HRZ75198.1"
                     /translation="MKELLKKFENKEPEIIFNWKDAETEAEGWTVINSLRGGAAGGGT
                     RMRKGLDMNEVLSLAKTMEVKFSVSGPAIGGAKSGINFDPNDPRKKGVLQRWYKAVSP
                     LLKSYYGTGGDLNVDEIHEVIPITEECGVWHPQEGVFNGHFKPTEADKINRIGQLRQG
                     VIKVIENPKFSPDVNRKYTVADMITGFGVAEAVRHYYDIYGGSVVGKKAVVQGFGNVG
                     SAAAFYLADMGAKVVGIIDREGGIINEDGFTFEEIKRLFLNKDGNKLVAPNMIPFEEI
                     NQKIWTIGAEIFAPCAASRLVQKGQVDQMIASGLEVISSGANVPFADKEIFFGSIMEE
                     TDAKVSLIPDFIANCGMARVFAYFMEKKVQMTDEAIFFDTSETIKRALEKAHSLNNSK
                     TNISATAFEIALKQLT"
     gene            complement(4507..5586)
                     /locus_tag="P5335_09725"
     CDS             complement(4507..5586)
                     /locus_tag="P5335_09725"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRZ75199.1"
                     /translation="MFKESYQVIGVMSGTSLDGIDLAHVHFTVKNNHWNYKIRETETI
                     PYTQEWLNKLKNAVDFSENELISLNEDYTDYLGNTIKKFIDKHKLLSVDAVCSHGHTI
                     LHQPQKGLTLQIGNLPKIAEIVEKTVVCDFRVQDVKLGGQGAPLVPIGDRLLFSEYDF
                     CLNLGGFSNVSFEEKGQRIAFDISPVNTVLNYYANQLGLEYDDKGKIASSGKLNPDLL
                     NELNNFDYYQKPYPKSLGFEFVKKIVLPLMEKYTVSIEDKMNTIIEHIAIQTDFSLPK
                     KEGKLLITGGGAYNDFLIEKMQKYLPKMQLIIPDSKTLEYKEALIFGLLGVLKLRNEI
                     NVLCSVTGAKKNHSSGFIYLPAECK"
     gene            5679..>6090
                     /locus_tag="P5335_09730"
     CDS             5679..>6090
                     /locus_tag="P5335_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRZ75200.1"
                     /translation="MDFNLTEEHLMIQQAARDFAQQELLPGVIERDEHSKFPTEQVKM
                     MAELGFMGMMVDPKYGGAGLDSVSYVLAMTEIAKVDASAAVIMSVNNSLVCAGMEKYC
                     SEEQKMKYLVPLAKGEVIGAFCLSEPEAGSDATSQ"
BASE COUNT         1907 a         1153 c          931 g         2099 t
ORIGIN      
        1 tgcttacttt tacttccaca acaaccgttc cttcatcatt acagtctgct tttacagctc
       61 ctggtttaga taattttctt ccgtttaatc cccaacttcc atttccggtg cctgttcctt
      121 ttccgctgcc ataataactg tttgaataaa tgctaccgtc tatttttcct tggtttcctg
      181 aattagtagt tgttccgtcg ccaccgcttg tttttccatc agacttttta acaccaccga
      241 ttaaggcatc tagttttgct tttttatctg cttcctcttg cttttttttc tgttcggcag
      301 cttttatttt tgcttcggca cttgcttttg cgtcagctat ttctttttgt ttttttaaag
      361 caattgcagc atcattatcg gctgtaagaa cattttctga tgctttactt ggtgttgtag
      421 gttctgaagt ttcttctact tttggttgct cagtgacttc tcttagttca gattttacca
      481 gttctgttgt gtttgtgttt cctgaaccag catcggtgtt accaaagtta atagcaattc
      541 cgttttccgg tggaggatcc atataggtta acccaaatac aaaaaacaac aataacaatc
      601 caattgtaat tgaagcactt aatgcaaatg attttttttc gtattcagtt tctaaaaact
      661 tcatgactaa gtttatttag gtcgaacggc taaaactact ttaatttgat ttctattggc
      721 aatatccaaa acatttactg ctttttcaat gggaacacct tcttcagctc ttaaaataat
      781 agctttatca tctttatttg aaaacaatgc taacaatttt gattctaaat cagcttcctg
      841 aacttggtct ttatcaataa aaaattctaa atctttatta atacttacag aaatattttt
      901 attgctatct gtttttcctt ttgctttagg cagaaccaaa tccaaagcat ttgtggttac
      961 catagtggat gtaagcatga agaaaatcaa aaacaagaat acaatatccg tcatcgacga
     1021 catattaaat tccggtgata ctttatttct acctcgaata ttcatattat gatggttcgt
     1081 ttaaaaggtc taagaaatca gttgcattgg cttccatttg atgaaccact ttgtctgttt
     1141 ttactaccaa gtgattataa cccataaatg aaatgatacc tacaaccaac ccggctactg
     1201 tagtagtcat ggctgtgtaa ataccctcag ctaaagcacc cacttcaatt tgtcctcctc
     1261 cgctggccat tttatgaaaa gcaagaatca taccaattac agtgcctaaa aacccaatca
     1321 tcggccctgc tccggatatg gtagctaaca tactaacatt tttttccaat ttatagattt
     1381 ctaatttacc ggcattttcg atggccgtat ttatgtcttc taaaggtttt ccaattcggg
     1441 atattccttt ttcagttagt ctggcaaccg gagaattggt ttgtgcacaa agcattttgg
     1501 cagcatcaat ttttccattc ataatattca ttttaatatt attcatgaaa ttcacatcaa
     1561 tttttgaagc ggcattaatt gccatcaaac gttcaaaata aagatataaa gcaaagaaaa
     1621 ataacaatcc taatgttatc ataatcattt gaccaccaat tccaccactt gaaacaagtt
     1681 cccagatgga taatgttttt tcaatgggtt gaggatctaa aactgcattt tgtgttgttg
     1741 ctaaactatc tattgcttgc atattatttg atttaaatta agtaacgcta aaatactgtt
     1801 ttaaattgtt taacgaacaa aatgctcaat aaacaaaaac gctattgctc cggcaataaa
     1861 tccggcaaaa gccaaccaag ttatcttttt aagataccaa atgaaatcga ttttctccat
     1921 tcccatagca gcaacacctg ctgctgagcc gataattaac atacttccgc ctgttccggc
     1981 tgaataagca ataaaatgcc aagctgggtg gtcaatttca taagtataca ttcccattga
     2041 ggcagctact aatggaacat tgtcaatcac agctgataag atccctaata aaattataac
     2101 aacatccata tttggaatgg cattactaat actttctgcg gcatagcgaa gagttcctac
     2161 ttcctgacct tgaatggttc cgaatactaa agattctaat gctgcaacag ccattaatat
     2221 tcctaaaaag aataaaatac ttgatatttc aattcttgat aaagcttttt gagcagaata
     2281 taaatgtttt ttgcttttat caaaatgatc ttcgggatga atatattctg aaactaacca
     2341 tacaatagct aaactaaaca tcatccctac ataagggggg agatgagtaa gtgttttaaa
     2401 aattggcaca aaaactatgg cacctaaacc cacccaaagc atagttttgc tacttaatag
     2461 tttctgctct tcttcattgt tttttatttc tatttgaatg tctcctttaa aaattttcat
     2521 tcgagaggct aatgcaaaag gaactacaaa acaaataact gctggaacaa ttatatactc
     2581 agataaacct acagctgata cttttttggc aatccataac atggttgttg taacatcccc
     2641 aattggtgac catgcacctc cggcatttgc agcaataatt atcaaggaaa cataccaaat
     2701 acgttcttct ctattttgaa ttaatttacg taataaagat attaaaacga ttgtagcagt
     2761 taaattatca attactgatg aaagaaaaaa tccaatgatt ccagtaatcc ataaaagttt
     2821 aattttacta gtggttttta ccatgccttt taacacgtcg aatccacgat gtaaatcaat
     2881 taattctaca attgtcattg ccccaataag aaatactata atttcagcgg ttttgccgag
     2941 atgatgcaaa agcaaacttt caaaaccgtg ttcttgttgg tgaatttcac cggaagcgat
     3001 attaaaaacg gagccataac catcaattac ggtaaaccaa ccttgatgaa atccaatggc
     3061 caaaaaagcc cacataacgg aggccattaa aagagcggga acagtcttgt ctaattttaa
     3121 aggatgttca agtgttatcg ataagtaacc aacaacaaat aataggataa taaaaagtgc
     3181 cattgaatat atattaagtt aattgtttca gtgcaatttc aaaagcagtt gcacttatgt
     3241 ttgtttttga attattgagt gagtgagctt tttctagggc tcttttaata gtctctgagg
     3301 tatcaaaaaa gatagcttca tctgtcattt gaactttttt ctccatgaaa taagcaaata
     3361 cacgagccat gccacaatta gcaataaaat caggaattaa actaactttc gcatctgttt
     3421 cttccatgat agaaccaaag aaaatttctt tatctgcaaa tggaacattg gcaccactag
     3481 aaataacttc taaaccggag gcaatcattt gatcgacttg gcctttttgt accaatcttg
     3541 aggctgcaca tggtgcaaaa atttcggctc caatggtcca aattttttga ttaatttctt
     3601 caaagggaat catgtttgga gcgaccaatt tattaccatc tttgtttaaa aataatcgtt
     3661 ttatttcttc aaaagtgaaa ccatcttcat ttatgattcc accttcacgg tcaataattc
     3721 caactacttt tgcccccata tcagccaaat aaaaggcggc ggcggaacct acattaccga
     3781 atccttgaac tacggctttt ttcccaacaa cacttccacc gtaaatatca taataatgac
     3841 gaaccgcttc cgcaacacca aatccggtaa tcatatcggc aacagtatat tttctattaa
     3901 cgtccggaga aaatttagga ttttctatca ctttaattac accttggcgt aattgaccta
     3961 tgcggtttat tttatctgct tctgttggtt tgaaatggcc attgaaaacc ccttcttgcg
     4021 gatgccaaac accacattct tctgtaattg gaataacttc gtgaatttca tcaacattca
     4081 aatcaccacc ggttccataa taacttttca aaagcggaga gactgctttg taccagcgtt
     4141 gcaaaacacc tttttttctt gggtcattag ggtcaaaatt gatgcctgat tttgctccac
     4201 caatagccgg gcctgatact gaaaatttta cctccatggt tttagccaat gatagaactt
     4261 cattcatatc taatcctttt cgcattctgg ttccaccacc ggcggcacca cctcgtagag
     4321 aattaattac tgtccatcct tcagcttctg tttcagcatc tttccaatta aatattattt
     4381 ctggttcttt attttcaaat tttttaagta attctttcat tcgggttaat ctgtttgttt
     4441 ttgcattgca aatttaatgg aacaaaatcg atttatttta tttttttact aaagttttca
     4501 agagttttat ttacattctg ctggcaaata aatgaaccca gagctgtgat ttttttttgc
     4561 tccggttaca ctgcaaagca cattaatttc atttcgaagt tttagaactc caagtaatcc
     4621 aaaaatcaaa gcttctttgt attccaaagt tttggaatca ggaataatta attgcatttt
     4681 tggtaaatat ttttgcattt tttctataag aaagtcatta taagcaccgc caccggttat
     4741 caataattta ccctcttttt ttggtagtga aaaatcggtt tgaatggcaa tgtgctctat
     4801 aatcgtattc attttgtctt caattgaaac agtatatttt tccattaaag gcaagacaat
     4861 ttttttgaca aattcaaatc ctaacgattt cggataaggt ttttgataat aatcaaaatt
     4921 atttaattca tttaataaat ctggatttaa ttttcctgaa cttgcaattt ttcctttatc
     4981 atcatattcc aaacctaatt gattggcata ataattcaaa acggtattta ccggcgaaat
     5041 atcaaatgct attcgttgcc ctttttcttc aaacgaaaca tttgaaaaac ctcctaaatt
     5101 caaacaaaaa tcatattcag aaaacaataa tcggtcacca ataggtacta aaggagctcc
     5161 ttgaccaccc aattttacat cttggactcg gaaatcgcag acaactgttt tctcaacaat
     5221 ttcagctatt ttgggtaaat ttccaatttg aagtgtcaat cctttttgag gttggtgtaa
     5281 aatagtatgg ccatgactgc aaacagcatc aaccgaaagt aatttatgtt tgtctataaa
     5341 ttttttgatt gtatttccta aataatccgt gtaatcttca tttagtgaaa tcaattcatt
     5401 ttcagaaaaa tcaactgcat ttttaagttt gtttagccat tcttgagtgt acggaatggt
     5461 ttccgtttct ctaattttgt agttccaatg attgtttttt acagtaaaat gaacatgagc
     5521 taaatcgata ccatccaatg atgttccgct catgactcca atgacttgat aggattcttt
     5581 aaacatggtc taaaattacg aaaacgtttg aaattatgac tttaatatta tacttttgcc
     5641 ttcaaattta agaaacaaac aaacaatatt ttatagttat ggattttaat cttaccgaag
     5701 agcatttaat gattcagcaa gctgcacgtg attttgctca acaagaatta ttgccgggtg
     5761 tcattgaaag agatgaacat tcaaaattcc caacagagca agtgaaaatg atggctgaat
     5821 taggctttat gggaatgatg gttgatccaa aatacggtgg agcaggttta gacagtgttt
     5881 cctatgtttt ggcgatgaca gaaatcgcaa aagttgatgc ttctgctgcc gttattatgt
     5941 cggttaataa ctcgttagtt tgtgccggaa tggaaaaata ttgtagtgaa gaacaaaaaa
     6001 tgaaatattt ggttccttta gcaaaagggg aagtgattgg agctttttgt ttatcagaac
     6061 cggaagcggg aagtgatgcc acatcacaaa
//
LOCUS       DAOTCW010000135         6058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_9634_length_6058_cov_54.944694, whole genome shotgun sequence.
ACCESSION   DAOTCW010000135 DAOTCW010000000
VERSION     DAOTCW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9634_length_6058_cov_54.944694"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1943)
                     /locus_tag="P5335_09735"
     CDS             complement(<1..1943)
                     /locus_tag="P5335_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ75201.1"
                     /translation="MRKNLLVFLFLNNFIVFAQEKGLVVGHILAEGQPVPFVSVVLKN
                     STTGTSSDENGNFSINVPAGKQTIVVQAIGFKTQEKNVNVPENGKIHVDFNLEESVFG
                     LNQVVVSATRGMQKRTEAPVIVTVTNSEVLQKAQAISLSEGLSFQPGLRMETNCQNCG
                     FSQVRMNGLDGAYSQILMDSRPIFSALNGVYGLDQIPTNMIERIEVVRGGGSSLYGSN
                     AIAGTINIITKEPVADSFEINTHYGLIDGTSPDKALTLNGTVLSDDLNVGIQLFGMLR
                     DRTQYDANDDNFTEIPSMENQTFGFKTFYRPFVRSKITAEFHTINEFRRGGENNFDLQ
                     PFEAYTTEQITSKMTGGGLTYEGYSEDEKNKYAFYFNTQQSKNDNFYGGRIDDVFGNM
                     NYEESLKGYGNTEVKTWIGGGQYIRKQDTFLNGSGTFTAGAEYKKEIMTDAKPGYNAF
                     VNQTLDILGIYAQEEWKVNEKLKVLAGLRADFHNAADEAVIFNPRLNILYNLKENLQW
                     RNSYAKGFRAPQVFSEDIHARIAAGEVALVELVDDLVSETSHSFLSSLDWTKVLANSE
                     AGLTFEAFYTRLNDPFVLEQISETIWEKRNGKGADVYGLNLEAKYAPGQKWQFQGGAT
                     IQKALYDEAVQWTENNVNTNQNF"
     gene            2030..2683
                     /locus_tag="P5335_09740"
     CDS             2030..2683
                     /locus_tag="P5335_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRZ75202.1"
                     /translation="MTYSEENYIKVIYHLSLVSPKGINTNAIAGMIESKASSVTDMVK
                     KLAEKELVDYQKYQGVTLTKKGLHAAKMIVRKHRLWEVFLVEKLAFSWDEVHDVAEEL
                     EHIHSEKLINRLEEFLGFPKEDPHGDPIPDRNGQIIKVEKQLLSDVSLHTKVICLGVK
                     DSSPSFLQYLDKHQISLGSIIEVVNKELFDMSLTLFVNNKEILISNKIASNLYVKLV"
     gene            2685..3503
                     /locus_tag="P5335_09745"
     CDS             2685..3503
                     /locus_tag="P5335_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009642623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRZ75203.1"
                     /translation="MFESIIAYLESIDPVLAALYATLFTWFLTALGASVVFFFKTMNR
                     LALDGMLGFTGGVMVAASYWSLLAPAIEMSHGEGFQKVFPAAFGFILGAFFLFGLDKI
                     LPHLHINFKETEGVKTPWQKTTLLVLAITLHNIPEGLAVGVLFGGVAAGVPEASIAGA
                     VVLAIGIGIQNFPEGIAVSMPLRRMGMSRKKSFMYGQSSALVEPIAGVVGAFAVTFFT
                     PILPYALAFAAGAMIFVVVEEVIPETQQANNTDIATLGFIGGFVVMMTLDVALG"
     gene            complement(3500..3625)
                     /locus_tag="P5335_09750"
     CDS             complement(3500..3625)
                     /locus_tag="P5335_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HRZ75204.1"
                     /translation="MDFQEIIAFVLLGIALVFLYNRFFGKKKTKKNCGSDDCGCH"
     gene            complement(3694..5934)
                     /locus_tag="P5335_09755"
     CDS             complement(3694..5934)
                     /locus_tag="P5335_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ75205.1"
                     /translation="MKTIFSFIFLSFSLTTFSQTTENVEDVALNDSVRKLNGVTLFGD
                     AVVGSKFKAKNVAGSTYFISPEELKLFHYNDINRILRTVPGVNIVEEEGFGLRPSIGL
                     RGTSPSRSSKITLMEDGILIAPAPYSAPAAYYFPTVNRIQSFEILKGGSQIQYGPNTT
                     GGAINMVSNQIPSQFSGRVVASMGNYDTRNTYVNLGDNFDNFGYLVEYNNRNSDGFKT
                     IDFSNKNTGFDGNDYVGKFRINTNAAAKIFQSLTLKAQLSEESSNETYLGLTDADFDS
                     NPYRRYLGSEADNIDTKHEQIMLTHLLIPSDNFTLTTKAYNNTFSRNWYKLDNVRING
                     TNYALSDILEDPITNQIGYDAITGTTDSPDNALRVKANNRSYGSKGIQTVANLKFASE
                     KFKHDLDFGVRYHEDYEDRFQWVDGFAIQNKALFRTSAGTPGTDANRILEAQAVAAHV
                     LYNFTYNKLVLSPGLRYENILSTSLNYGNADVDRTGANLVKINNRVDVFIPGMGALYK
                     FNDNYTGFASLHKGFSPPGAVEGADVENSINYEAGFRFTKKALSGEIIGFYNDFSNLQ
                     GADTNASGGTGTGDLFNAGKAEVNGVEVLVSYNILNNENSKLKLPITATYTFTNTELK
                     SNFASGTEAWGEIEVGDEIPYIPKNLFAIVAGLEYQKFTISLSGKYTDEFRTVAGQGP
                     IPANQLIKSNFIVDASAKYFLTEKISLMTNLVNILDKEYAVSRVPSGLRPGMPFGINF
                     GITAQF"
BASE COUNT         1874 a         1106 c         1062 g         2016 t
ORIGIN      
        1 aaaaaattct gattcgtatt cacattgttt tctgtccatt gaacagcttc atcatacaat
       61 gctttttgaa tcgttgctcc accttgaaac tgccattttt gacccggggc atattttgct
      121 tctaaattta atccgtaaac atcggctcct tttccgtttc ttttttccca aattgtttcc
      181 gaaatctgtt ccaaaacaaa aggatcattc aatcgcgtat aaaaagcttc aaaggtcaaa
      241 cccgcttctg aattggctaa aacttttgtc caatctaatg aggataaaaa cgaatgagaa
      301 gtttctgaaa ccaaatcatc taccaattcc accaaagcca cttcaccggc ggcaattcgg
      361 gcatgaatat cttccgaaaa aacctgtgga gctctaaatc ctttggcata actgtttcgc
      421 cactgtaagt tttctttcag gttatacaaa atattcaatc gcggattaaa aatgaccgct
      481 tcatcagccg cattgtgaaa atcggcacgc aaccctgcca gtacttttag tttttcatta
      541 actttccatt cttcctgtgc ataaataccc aaaatatcca aagtttgatt tacaaaagca
      601 ttgtatcccg gtttagcatc ggtcatgatt tcttttttgt attcggctcc ggctgtaaat
      661 gttccgcttc catttaaaaa agtatcttgt ttgcgaatgt attgtccacc accaatccaa
      721 gttttgactt cggtgttacc ataacctttt aagctttctt cataattcat gtttccaaac
      781 acatcgtcaa ttcttcctcc gtaaaagtta tcattttttg attgttgcgt gttgaaataa
      841 aaggcatatt tgtttttttc atcttccgaa taaccttcat aagttaatcc tcctccggtc
      901 atttttgagg taatttgttc ggttgtataa gcttcaaagg gttgcaaatc gaagttgttt
      961 tcacctcctc ttcgaaattc gttgatggta tgaaattcgg ctgtaatttt acttcgcaca
     1021 aacggacgat agaaggtttt aaaaccgaaa gtttgatttt ccatcgatgg aatttctgtg
     1081 aaattatcat catttgcatc atattgcgtt cggtcacgaa gcatcccaaa tagttgaata
     1141 cccacattta aatcgtcact taaaacagtt ccattcaacg ttaatgcttt atcaggagaa
     1201 gttccatcaa ttaatccgta atgcgtgttg atttcaaatg aatcagccac aggttctttg
     1261 gtaataatat tgatagttcc tgcaattgca ttcgagccat ataaagacga acctccgcca
     1321 cgaaccactt caatacgttc aatcatattg gttggaatct gatctagtcc ataaacacca
     1381 tttagggctg aaaaaattgg acgactgtcc attaatattt gcgaataagc tccgtccaag
     1441 ccattcatac gaacttgtga aaatccgcaa ttttgacaat tagtttccat tcgcaatccg
     1501 ggttgaaaac tcaagccttc cgataaacta atggcttggg ctttttgcaa cacttccgaa
     1561 tttgttacgg ttacaatcac cggtgcttcc gttctttttt gcatgcctcg tgttgctgaa
     1621 acaacaactt ggtttaatcc gaaaacactt tcttctaaat taaaatccac atgaattttg
     1681 ccattttcag gaacgttaac atttttttcc tgcgttttaa aaccgattgc ttgaacaaca
     1741 atggtttgct ttccggcagg aacattgata gaaaaatttc cattttcatc agaagatgtt
     1801 cctgtagttg agtttttcaa aacaacactc acaaaaggaa caggttgacc ttccgctaaa
     1861 atgtgaccaa ccactaatcc tttttcttga gcaaaaacga tgaaattgtt tagaaataga
     1921 aaaacaagca gattttttct catagcaaaa aataatttta gacaaaacta aaaaatattt
     1981 tactcatatc aaattaagtt agtatatttg ttatcaatta gttgaattta tgacctattc
     2041 ggaagaaaat tatattaagg taatttatca tctttctttg gtttctccca aaggaatcaa
     2101 taccaacgct attgccggaa tgattgaaag caaagcttcc tcggtgacgg atatggtgaa
     2161 aaaattagcc gaaaaagaat tggttgatta ccaaaaatac caaggagtaa cgttgaccaa
     2221 aaaaggactt catgcagcaa aaatgattgt gcggaaacat cgtttgtggg aagttttttt
     2281 agttgaaaag ttagctttct catgggatga agtgcatgat gtagccgaag aattggaaca
     2341 tattcactcg gaaaaattaa ttaatcgatt ggaagaattt ttaggatttc caaaagaaga
     2401 tccacacgga gatccgattc cggatagaaa cggacaaatt attaaagttg aaaaacaatt
     2461 gctttcggat gtttcgttac acacaaaagt catttgtttg ggcgtaaaag acagttcacc
     2521 tagtttttta caatatttag ataaacacca aatttcgttg ggttcaatca tagaagtagt
     2581 taacaaagaa ctttttgata tgtcgttaac cttatttgta aataacaaag aaatcttgat
     2641 ttcaaataaa attgccagta atttatatgt aaaattagtt taacatgttt gaatcaatta
     2701 tagcttattt agaaagtata gatccggtat tggccgcttt atatgcaacc ttatttactt
     2761 ggtttttaac tgctttagga gcatcggttg ttttcttttt taaaaccatg aatcgactgg
     2821 ctcttgatgg aatgctcgga tttacaggag gtgttatggt tgccgccagt tattggagtt
     2881 tgttggcacc tgccattgaa atgtcgcatg gagaaggttt tcaaaaagtg tttccggcag
     2941 ctttcggatt tatattaggg gcttttttct tattcggatt agacaaaata ttacctcatc
     3001 tacacatcaa ttttaaagaa acagagggcg ttaaaacacc ttggcaaaaa acgactcttt
     3061 tagttttagc gataacatta cataatattc cggaaggctt agctgtaggt gttttatttg
     3121 gtggtgttgc tgcaggagtt cccgaagcat ccattgcggg tgctgtagta ttggcaatag
     3181 ggattgggat tcaaaatttt cctgaaggaa tcgctgtttc gatgccgttg cggcgaatgg
     3241 gaatgagccg taaaaagagt tttatgtacg gacaatcttc tgcgttagtg gaaccaatcg
     3301 ccggagtagt tggtgctttt gcggtaactt tttttacacc tattttacct tacgcattgg
     3361 cttttgcggc cggagcaatg atatttgtag tggttgaaga ggtgattccg gaaacgcaac
     3421 aagccaataa taccgacatt gcaaccttag gttttatagg cggttttgtg gtaatgatga
     3481 cacttgatgt ggctttgggt taatgacaac cacaatcatc tgaaccacaa ttttttttag
     3541 ttttcttttt tccgaaaaat ctattgtaaa gaaagaccaa agcaattcct aaaagtacaa
     3601 aagcaattat ttcttgaaaa tccatagggt aaagatataa aaagagtcca ttagaaacta
     3661 aagaactctc ttaaacaaac atgaaaacca aatttaaaat tgtgcagtta ttccgaaatt
     3721 gattccaaac ggcataccgg gacgtaaacc ggatggaact cttgaaacag catattcttt
     3781 atctaaaata ttcaccaaat tggtcattaa acttattttt tccgttaaaa agtatttggc
     3841 agaagcatct actataaaat tacttttgat taattgattc gcaggtatgg gtccttgacc
     3901 ggcaacggtt ctaaattcgt cagtatattt tccgcttagc gaaatagtaa atttttggta
     3961 ttccaatcca gccacaattg caaatagatt ttttggaatg tatggaattt cgtcaccaac
     4021 ttcaatttca ccccaagctt cagttccact tgcaaaattg ctttttaatt cagtatttgt
     4081 aaaagtataa gtggctgtga ttggcaattt taatttactg ttttcattat ttaaaatgtt
     4141 gtaagaaact aaaacttcta caccatttac ttctgctttt cctgcattga acaaatcgcc
     4201 ggttccggtt ccccctgaag cattcgtgtc tgcaccttgc aagttgctga agtcattgta
     4261 aaaacctatt atttctcctg aaagtgcctt tttagtgaaa cgaaatccgg cttcgtagtt
     4321 gatactgttt tcaacatctg caccttcaac agctcccgga ggcgaaaatc ctttgtgtaa
     4381 acttgcaaaa ccggtataat tatcattgaa tttgtaaagt gcacccattc caggaatgaa
     4441 aacatcaacg cggttattta ttttaaccaa atttgctcca gttctgtcga catcggcatt
     4501 tccataattt aaactagtgg ataaaatatt ttcgtaacgc aaacccggtg aaagcactaa
     4561 cttattatag gtaaaattat aaagtacatg agcggcaact gcttgtgctt ccaaaattcg
     4621 attagcatcg gttccgggag ttccggcaga agttctaaac aaggctttgt tttgaatcgc
     4681 aaaaccatcc acccattgaa aacgatcttc gtaatcttca tggtagcgaa caccaaaatc
     4741 taaatcatgt ttgaactttt ctgaagcaaa ttttaggtta gcaacagttt gaattccttt
     4801 ggaaccgtaa cttctgttgt ttgcttttac acgtaaagca ttgtcaggcg aatcagttgt
     4861 tccggtaatt gcatcgtagc cgatttgatt ggttataggg tcttctagta tatcacttaa
     4921 agcgtaattg gttccgttaa ttcgaacatt gtctagtttg taccaatttc tcgaaaatgt
     4981 attgttgtat gcttttgtag ttagcgtgaa attatcggac ggaatcaaaa gatgcgtcaa
     5041 catgatttgt tcgtgtttcg tgtcaatatt atctgcttca gagcctaaat atcttctgta
     5101 aggattcgaa tcaaaatcgg catcggttaa accaagatat gtttcattgg atgattcttc
     5161 tgataattga gcttttagag taagtgattg aaaaattttc gcagctgcat tggtgttgat
     5221 tctgaatttt ccaacataat catttccatc gaatccggta tttttattac taaagtctat
     5281 tgttttaaat ccgtcagaat ttcgattgtt gtattctacc aaataaccaa agttatcaaa
     5341 attgtcacct aagttcacat aagtatttcg agtatcataa ttgcccatac ttgccacaac
     5401 tcttcctgaa aattgcgacg gaatttgatt ggaaaccatg ttaatggctc caccggttgt
     5461 attaggacca tattgaattt ggcttccgcc ttttaaaatt tcaaaactct gaatacggtt
     5521 tacggttgga aaataatatg ccgcaggagc tgaataagga gcaggtgcaa ttaaaattcc
     5581 gtcttccatt aaagtgattt ttgagcttcg tgacggactg gttccacgca aaccaatact
     5641 tggacgaagt ccgaaacctt cttcttctac aatatttacg ccgggaactg ttcgtaaaat
     5701 tctgttgata tcattgtaat gaaatagttt caattcttcc ggagaaatga aataggtaga
     5761 accggcaacg tttttggctt tgaatttact tccgacaacc gcatcgccaa aaagggttac
     5821 accatttagt tttcgaacag aatcatttag agctacatct tcaacatttt ctgttgtttg
     5881 cgaaaaagtg gtaagtgaaa aagaaaggaa aataaaagag aaaatagttt tcatttttgt
     5941 tatttagaat taatctacat tgcaaaaata ataactattt ttatttagac aaattataaa
     6001 taacaaaatt tatcaattaa tttataacca attgaaaatg aaaaatttaa ttaaaata
//
LOCUS       DAOTCW010000136         6036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_9676_length_6036_cov_44.145293, whole genome shotgun sequence.
ACCESSION   DAOTCW010000136 DAOTCW010000000
VERSION     DAOTCW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9676_length_6036_cov_44.145293"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..2156)
                     /locus_tag="P5335_09760"
     CDS             complement(81..2156)
                     /locus_tag="P5335_09760"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRZ75206.1"
                     /translation="MKTVMTIFENSIIKGSYFLLFFFAFGGYAQTFTDSNLPIVIITT
                     DINPDTNQPYEIPDEPRVFGSMKIIKHPDGSRNYMSDSNTAEFLNYDGRINIEIRGSS
                     SQALPKKPYGLTTLKPDNISNNNVSLLGMPSENDWVLNSLAFDPSLIRDHLSYAISRQ
                     MGNYAPRTVYCEVVINGEYIGLYLLQEKIKADSNRVNILKITTTDTALPNVTGGYITK
                     ADKTTGDDPIAWSMPSYTGVTDYIHDLPKPEDVTPQQNFYIYNQFSLLANTSSIGNIA
                     ITNGYPSIIDIPTFVDFMLSNELASNVDGYQYSTYFHKERAGKLRAGPIWDFNLTYGN
                     DLFLWGYDRSKTNVWQFGNEDNEGSKFWKDLFNNSTFKCYLSKRWNELKQPGNAMNLA
                     FLNTFIDETVTYINEAKIRENQKWGTIPNQELEITNLKSFLSIRTTWITNNIGSYSAC
                     SSVSTPSLVITKINYNPSTSIEYPESNDSEFIEIKNTGLQNVPLSGIYFKELGISYSF
                     PVGSTILQGQSIYLASNADVFLAKYGISAFGQFTRNLSNKSQKLILADAFGNTIDYVE
                     YMDSSPWPTLADGNGYYLQLISTDLDNSLASSWTTTADQSLSSIEYTFTSDLKIYPNP
                     VNSILNVQATQRITKIEIFDVFGKKLQTMIDDSAFIHLKMDSFAKGIYFLKIYGGEMS
                     FKVEKVIKQ"
     gene            complement(2270..5122)
                     /gene="carB"
                     /locus_tag="P5335_09765"
     CDS             complement(2270..5122)
                     /gene="carB"
                     /locus_tag="P5335_09765"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRZ75207.1"
                     /translation="MPRDTSIKSVLIIGSGPIVIGQACEFDYAGSQSARSIREEGIEV
                     ILINSNPATIMTDPTMADHVYLLPLTTKSIIHILKKHPNIDAVLPTMGGQTALNLCLE
                     ADEKGIWEDFGVRLIGVDINAINITEDREQFKQLLERIGVGAAPAKTATSFLKGKEIA
                     QEFGFPLVIRPSFTLGGTGAAFVHTKEEFDELLTRGLEASPIHEVLIDKALLGWKEYE
                     LELLRDQNDNVVIICSIENMDPMGIHTGDSITVAPAMTLSDTTFQKMRDMAILMMRSI
                     GNFAGGCNVQFAVSPDEKEDIVAIEINPRVSRSSALASKATGYPIAKIASKLALGYHL
                     DELQNQITKSTSALFEPTLDYVIVKIPRWNFDKFEGSDRTLGLQMKSVGEVMGIGRSF
                     QEALHKATQSLEIKRNGLGADGKGYKNYEQIIEKLTYASWDRVFVIYDAIQMGIPLSR
                     IHEITRIDMWFLKQYEELYLLEKEISNFSIDTLSKELLLEAKQKGFADRQIAHMIRCL
                     ESQVYKKREELSVNRVYKLVDTCAAEFKAQTPYYYSTFEADIETPTGERYVHNESVVS
                     EKKKVIVLGSGPNRIGQGIEFDYSCVHGVLAAKECGYETIMINCNPETVSTDFDTADK
                     LYFEPVFWEHIYDIIRHEKPEGVIVQLGGQTALKLAEKLDRYGIKILGTSFDALDLAE
                     DRGRFSELLKELKIPFPQFGVAENADQAIALADVLDFPLLVRPSYVLGGQGMKIVINK
                     QELEEHVIDLLKNIPGNKLLLDHYLDGAIEAEADAICDGENVYIIGIMEHIEPCGVHS
                     GDSNATLPPFNLGEFVMQQIKDHTHKIALALKTVGLINIQFAIKDDIVYIIEANPRAS
                     RTVPFIAKAYGEPYVNYATKVMLGENKVTDFTFNPQLKGYAIKQPVFSFSKFHGVNKK
                     LGPEMKSTGESILFIDDLKDDQFYELYSRRKMYLSK"
     gene            complement(5224..5385)
                     /gene="rpmH"
                     /locus_tag="P5335_09770"
     CDS             complement(5224..5385)
                     /gene="rpmH"
                     /locus_tag="P5335_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRZ75208.1"
                     /translation="MSKRTFQPSKRKRRNKHGFMDRMATANGRKVLARRRAKGRHKLT
                     VSCEPRHKK"
     gene            5543..>6036
                     /locus_tag="P5335_09775"
     CDS             5543..>6036
                     /locus_tag="P5335_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRZ75209.1"
                     /translation="MFHKNIKLVLAAVLIAVGIWQFTENNIGNGIFLILLTFFPIFLY
                     FKNEIILLAFLKLRKQDFEGAKRWLDKIKKPEGALVKKQQGYYNYLHGLMVSQTNLNQ
                     AEKFFKKAIELGLSMDMDLAVAKLNLAGIAMSRRRKIEATNLLNEANKLDKQNMLKEQ
                     ITMM"
BASE COUNT         2008 a         1083 c          902 g         2043 t
ORIGIN      
        1 agctcaaaaa aactcaaaat aaacaagttt gaatttgttt gttttgagtt ttttaaattt
       61 aattctattt ttttaaacac ttattgtttt attacttttt caactttaaa actcatttct
      121 ccaccataaa tttttagaaa ataaattcct tttgcaaatg aatccatttt caaatgaatg
      181 aatgcggaat catcaatcat ggtttggagt ttttttccaa agacatcaaa aatttcaatt
      241 ttagtaatcc gttgagtagc ttgaacgttc aaaattgaat taaccggatt tggataaatt
      301 tttaaatcgg aagtaaaagt atattcaatt gaggataggg attggtcggc tgtagtggtc
      361 caacttgaag ctaaactatt gtctaaatcc gtacttatca attgcagata gtacccattt
      421 ccgtctgcta aagttggcca tggtgatgaa tccatatatt ctacataatc aattgtattg
      481 ccaaatgcat cggctaaaat aagcttttga gatttatttg ataaatttct agtaaattgt
      541 ccaaatgcag aaataccata tttagctaaa aatacatctg cattactggc taaataaata
      601 ctttgccctt gtaaaatagt cgaaccaacc gggaaactgt acgaaattcc taattcttta
      661 aaataaattc cggaaagggg cacgttttgt aatccggtat ttttaatttc aataaattct
      721 gagtcattgc tttccggata ctcaattgaa gtagacggat tatagttaat ctttgtgatt
      781 accaatgacg gagttgaaac cgaggaacaa gccgaatacg aacctatatt attagtaatc
      841 caagtagtcc ttatacttaa aaatgatttt aaattcgtaa tttcaagttc ctgatttggt
      901 atggtacccc atttttgatt ctctctgatc tttgcttcgt ttatataagt taccgtttca
      961 tcaataaatg tgtttaaaaa tgccaaattc attgcatttc cgggttgctt cagttcattc
     1021 caacgctttg ataaataaca tttaaaagtt gaattattga acaaatcttt ccaaaatttt
     1081 gaaccttcat tatcctcatt gccaaattgc caaacatttg ttttacttcg gtcatatccc
     1141 caaagaaaca aatcattacc gtacgtaaga ttaaaatccc aaataggtcc tgctctcagt
     1201 ttaccggctc gttctttatg aaaataagtg ctgtattgat aaccatcaac attagatgcc
     1261 aactcattgg ataacataaa atcaacaaaa gttggaatat caattattga aggataacca
     1321 ttagtaatag ctatattacc tattgaagaa gtgtttgcca ataaactaaa ctgattataa
     1381 atataaaaat tttgttgggg agtgacatct tcaggtttag gcaaatcatg aatataatcc
     1441 gtaacaccgg tataagatgg catagaccaa gcaatagggt catcgccggt tgttttatcg
     1501 gcttttgtaa tatatccacc ggtaacatta ggcaaagcag tatcagtagt cgtaattttt
     1561 aaaatattta cacgattaga atctgcttta atcttttcct gtaaaaggta taaaccaatg
     1621 tactcgccgt taataactac ttcacaataa acagttctcg gtgcataatt tcccatttgt
     1681 cttgaaatgg catacgataa atggtctcta atcaaagaag gatcaaaagc taaactattg
     1741 agaacccagt cattttcgct tggcattcct aacaaactaa cattgttatt actgatatta
     1801 tcaggtttta aagttgttaa tccataaggt ttcttaggta aagcttgtga ggaagaacct
     1861 cgtatttcga tatttatgcg tccatcataa tttaaaaatt cagcagtatt actatctgac
     1921 atataatttc gggaaccatc cggatgctta attatcttca ttgaaccaaa aactcttggt
     1981 tcgtccggaa tttcataagg ttgattagta tcgggattaa tatccgttgt aatgataaca
     2041 attggtaaat tactatctgt aaaagtttgt gcataacctc caaaagcgaa aaaaaacagt
     2101 aagaaataag atcctttaat aatagaattc tcaaaaatag tcattactgt tttcatcaga
     2161 tttattattt tatcaaattt aggcataagt aagctaacaa ttaacttaga aagactacaa
     2221 atccctattt tgcaaaaaaa gaccttaaaa aaggtctttt gaatgagttc tatttactta
     2281 aatacatttt gcgtctggaa tacaattcgt aaaattggtc atctttcaaa tcatcaataa
     2341 acaaaatact ttctccggtt gatttcattt caggtccaag tttcttgttt acaccatgaa
     2401 atttactaaa ggagaatacc ggttgcttaa tggcatatcc ttttaattgt ggattaaaag
     2461 taaaatcggt aactttattt tcacctaaca tcacttttgt agcataattc acataaggtt
     2521 ctccataggc ttttgcaata aaaggaactg ttctggatgc tcttggattg gcttcaatga
     2581 tgtatacaat atcatctttt atggcaaact gtatatttat taatcctact gttttgagag
     2641 ctaaagcaat tttgtgagta tgatctttta tttgttgcat cacaaattca cctaaattaa
     2701 acggtggcaa tgtagcatta ctatctccgg aatgtacacc acatggttca atatgttcca
     2761 taattccgat gatataaaca ttttcaccat cacaaatagc atctgcttct gcttcaatgg
     2821 ctccatccaa ataatggtct aataataatt tatttccggg tatatttttt aataaatcaa
     2881 taacatgctc ctctaattcc tgcttattga taacgatttt cattccttgt ccacctaaaa
     2941 cataagaagg tctaaccaat aatgggaaat ccaacacatc tgctaaagca atagcctgat
     3001 cagcattttc tgcaacacca aattgaggaa atggtatttt caattctttc agtaattcag
     3061 aaaaacgacc tctatcttcg gctaaatcta aggcatcaaa actagttcct aaaatcttaa
     3121 ttccgtaacg atctaatttt tcagctaatt ttaaagcggt ttgaccacct aattgaacaa
     3181 ttacaccttc cggcttttca tgacggatga tgtcgtaaat atgttcccaa aaaacgggtt
     3241 caaaatataa tttatccgct gtatcaaaat cggtagaaac agtttccgga ttacaattaa
     3301 tcattattgt ttcataacca cattctttag ccgctaaaac gccatgcaca caggaataat
     3361 caaattcaat accttgccca atccgatttg gccccgaacc caaaactatc acttttttct
     3421 tttcagaaac tacactttca ttatgaacat acctttctcc cgttggagtt tctatatctg
     3481 cttcaaaagt tgagtaataa taaggtgttt gagctttaaa ttcggcggca caagtgtcga
     3541 ctaatttata aacacgattc acggaaagtt cttctctttt tttatagacc tgactttcca
     3601 aacaacgaat catgtgagca atttgtctgt cagcaaaacc tttttgcttt gcttctaata
     3661 gcaattcttt ggataaagta tcaattgaaa aatttgaaat ctctttttct agtaggtaaa
     3721 gttcttcata ttgtttaaga aaccacatat ctattctggt tatttcatga attctactta
     3781 atggaattcc catttgaata gcatcataaa taacaaaaac cctatcccaa ctggcataag
     3841 tcagtttttc tataatttgt tcataatttt tgtacccttt cccatcagca cctaatccat
     3901 ttcgtttaat ctctaaagat tgggtagctt tatgaagagc ctcctgaaaa gagcgaccaa
     3961 ttcccattac ctcccctacc gatttcatct gtaatcctaa tgttctgtca gaaccttcaa
     4021 atttatcgaa attccaacgt ggaattttta caatcacata atctaaagtc ggctcaaaaa
     4081 gagctgaagt tgattttgtg atttgatttt gcaattcgtc taaatgataa cctaacgcta
     4141 atttggaagc aatttttgca attggataac cggttgcttt agaagccaag gcagaagaac
     4201 gtgacacgcg tggattgatt tcaatagcaa caatatcctc tttttcatcc ggtgaaacgg
     4261 cgaattgtac gttacaacct ccggcaaaat taccaatact tctcatcatc aaaatggcca
     4321 tatctctcat tttctgaaaa gtagtatcag ataaagtcat agccggggca accgtaatac
     4381 tgtctccggt atgaattccc atcggatcca tgttttcgat ggaacatata attactacgt
     4441 tatcattttg atcacgaagt aattccaatt catattcttt ccaacccaat aaagctttat
     4501 caatcaaaac ttcgtgaatg ggtgatgctt ctaacccacg tgttaaaagt tcatcaaatt
     4561 cttcttttgt atgaacaaaa gctgctccag ttcctcctaa agtgaacgaa ggacgaataa
     4621 ctaaaggaaa tccaaattcc tgtgcaattt cctttccttt taaaaaggaa gtagcagtct
     4681 ttgccggtgc agctcctact ccaattcgtt ctaacaattg tttgaattgt tctctgtctt
     4741 ccgtaatgtt aatggcatta atatcaacac caattaatcg taccccaaaa tcttcccaaa
     4801 ttcctttttc atccgcttct aaacacaaat tcaatgcagt ttgtccaccc atagtaggaa
     4861 gaacagcatc gatatttgga tgttttttta gtatatgaat aattgattta gtcgttaaag
     4921 gcaataaata aacatgatcg gccatggttg ggtcggtcat aatagtagcc ggattggaat
     4981 taatcaaaat aacttcaatt ccctcttcac gaatggaacg ggctgattgt gagcctgcat
     5041 aatcaaattc acaggcttga ccgattacaa tgggccctga acctataatt aaaactgatt
     5101 tgatggaagt atctcttggc ataatattgt gatatagtag tttagttgtt ttgtgtcgac
     5161 aagttataaa aaaaaggcgt tactaaaaaa agtaacgcct taatatttga ttattgctaa
     5221 tcattatttt ttgtgtcttg gttcgcagga aacagttaat ttatgtcttc ctttagctct
     5281 acgacgagca aggacttttc taccatttgc agtagccatt ctatccataa acccgtgttt
     5341 atttcttctt tttcttttcg atggttgaaa cgttctttta ctcattgtga tgtatcttta
     5401 aaaatcgtgt ataataatct tcatttaggt tcttcaaaaa ccgagtgcaa atatacaaag
     5461 actttttttt ctcacaagtg gttttataaa aatattttca attcctttta ttacctttgc
     5521 aaacacaaat aataaagtca ttatgtttca caaaaatatc aaattagttc ttgccgcagt
     5581 attgattgcc gttggtatat ggcagttcac tgaaaacaac atcggaaatg gaatattcct
     5641 tatcttatta actttttttc cgattttttt atatttcaaa aacgaaatta tcttattagc
     5701 ctttctaaaa ctaagaaagc aagattttga aggagcaaaa agatggttag ataaaataaa
     5761 aaaacctgaa ggagctttgg ttaaaaagca acaaggctat tataattatt tgcacggatt
     5821 gatggtatca caaaccaatt taaaccaagc agaaaaattc ttcaaaaaag caattgaatt
     5881 aggtttatca atggatatgg atttagcggt agccaaatta aatttagcag gaatagccat
     5941 gtccagaaga agaaaaattg aagcaactaa tttacttaac gaagcaaata aattagacaa
     6001 acaaaacatg ttgaaagaac aaatcaccat gatgaa
//
LOCUS       DAOTCW010000137         6018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_9722_length_6018_cov_43.452121, whole genome shotgun sequence.
ACCESSION   DAOTCW010000137 DAOTCW010000000
VERSION     DAOTCW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9722_length_6018_cov_43.452121"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="P5335_09780"
     CDS             <1..293
                     /locus_tag="P5335_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRZ75210.1"
                     /translation="LSDNNLIIKRIFNLDTNAYSEGALDVKTKELLGLVASAVLRCDD
                     CIKYHLETSYKHGITREEMMEAMGIATLVGGTIVIPHLRRAYEFWEALENQQ"
     gene            399..1139
                     /gene="lptB"
                     /locus_tag="P5335_09785"
     CDS             399..1139
                     /gene="lptB"
                     /locus_tag="P5335_09785"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRZ75211.1"
                     /translation="MKLRAENIVKTYKGRSVVKGVSVEVNQGEIVGLLGPNGAGKTTS
                     FYMIVGLVKPNSGKIYLDDLEITDFPMYKRAQNGIGYLAQEASVFRKLSIEDNILSVL
                     QLTKLSKAEQEAKMESLIDEFGLQHIRTNRGDLLSGGERRRTEIARALATDPKFILLD
                     EPFAGVDPVAVEDIQRIVAQLKNKNIGILITDHNVQETLAITDKTYLMFEGGILKAGI
                     PEELAADEMVRKVYLGQNFELRKKKLEF"
     gene            complement(1147..2004)
                     /locus_tag="P5335_09790"
     CDS             complement(1147..2004)
                     /locus_tag="P5335_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HRZ75212.1"
                     /translation="MRKPVKRKPTVKRKSSRKKSPFSGKFLSIGALLLFLLFIGFAGY
                     HYREALAYYFSFRSRHELKTANTTKIEGARIFQVLSKHSSNVFGLDVSEYQGDIHWEE
                     VKTVEDTFQLEFAFIRATAGKDRIDAKFKHNWSKAKEYGFVCGAYHYYRPNENSIKQA
                     DNFIKTVTLKKGDFPPILDIENLPKTQSIDSLKVGLRRWLTKVENHYGVKPIIYSGES
                     YYKDFLKKEFKGYSFWIANYNFFVEQIKDDWLFWQFTEKATIPGIKGPVDINIYNGTP
                     KMLNYSVLQ"
     gene            complement(2034..2765)
                     /locus_tag="P5335_09795"
     CDS             complement(2034..2765)
                     /locus_tag="P5335_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRZ75213.1"
                     /translation="MNIKSKIPNFITLLNLLSGCIALVFVAGNRFDIAFFFVCLGIFF
                     DFFDGFFARLFKVQSPLGVQLDSLADMVTSGAVPGFVMFQLIFKYQNEGISVFDDFFP
                     TNHILPLAGFLITLGSCYRLAKFNIDTRQTNSFIGLPTPANALFILSIPLIIDSTTFL
                     GLKEFLSNPYFLMAISAFSAYILNAEIPLFSLKMKNFSWETHKIQVLFLLFSLILLFS
                     FKILGLPLVILLYVLLSVIQNKTAQ"
     gene            2970..>3985
                     /locus_tag="P5335_09800"
     CDS             2970..>3985
                     /locus_tag="P5335_09800"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRZ75214.1"
                     /translation="MLFITSFSYSQSIRKYSNEFMNIGVDAAALGMSNAVTANSNDVN
                     SGYWNPAGLIHLEDNQLSLMHASYFANIAQYDYAAFAMPIDDRSAWGFSLIRFGVDDI
                     LDTTQLIDSEGNIDYNRINLFSTADYGATFSYARSLQVPGWQYGVNAKIIRRIIGKFA
                     NSWGFGIDVGIQFERNDWKFGLMVRDITTTYNVWNIDEEKFQSIADAIPGENQELPES
                     TEITLPKAQFGISRKFEFNYDYTLLSSINLNMQFAKTNDIISTDAVSIDPAFGFEFGY
                     TDLVFLRAGIGNFQNIQQIDNSEKVGFQPNIGLGFKYKGIQIDYALTDIGDQSVALYS
                     NVFSV"
     assembly_gap    3986..4085
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4086..5024
                     /locus_tag="P5335_09805"
     CDS             <4086..5024
                     /locus_tag="P5335_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRZ75215.1"
                     /translation="DVVYNFGMFDFSTPNFYLKFVKGDMQYYVANSSFEHFMYSYRME
                     NRSVFEQELNLTTEQKQKLFDNLNQAVFSEGRFYTYKFIDKNCTTMVVDKINDELGKP
                     VIKKVGNIEESYRDVLNPYLANLFYEKLGINIIFGHRTDEKAEQLFLPIELLKSLEQL
                     EFNNKPLAGKTVTLFQQDVTLLSNSWWNTIYTFLLFLVLVVVSNRNWIYLLFLFLLGS
                     LGIFLSLVGFYSLHREVLQNYNVLLFNPFLIGLVYCYIRKNLIWFKRIFYFVLTSFSI
                     YLLFMLNKTHLFQMLPILLTIVIILFKLRKRILSTQ"
     gene            complement(5029..>6018)
                     /locus_tag="P5335_09810"
     CDS             complement(5029..>6018)
                     /locus_tag="P5335_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRZ75216.1"
                     /translation="SHRFEKKVILICEKELADELILGLKSVDPHYTIVALVNTDENSD
                     FSILNKTVSIISVAGLEKFVSKNSISEIVIATQKTEEITVDLYNKLLLLLENGFIIRE
                     YTHVYENLTQRIPVQYVARDFYRYFPFSRSNQNQLYLLFVRIFEYVIAIIGITLGLFF
                     VPFILIGNFIGNRGKLFYSQIRVGKNGEEFRIIKFRTMVKNAESAGAVFATTNDARIT
                     PFGKFLRKSRIDEFPQFINILLGHMAVIGPRPERPVFVNEIAEIMPFYQTRHVVKPGL
                     TGWAQVNYSYGDSIDDSLIKLQYDLYYIKHRSIFLDFNIMLKTLSTILFFRGQ"
BASE COUNT         2106 a          905 c          946 g         1961 t
ORIGIN      
        1 tactttctga taataattta attatcaaac gtatttttaa tttagacacc aatgcttatt
       61 cagaaggtgc gttggatgta aaaacaaaag aattattagg cttagtcgct tcggccgttt
      121 tacgttgtga cgattgcatc aaataccatt tagaaaccag ttataaacac ggtataactc
      181 gtgaagaaat gatggaagct atgggaattg caaccttggt tgggggaaca atagttattc
      241 cacatttacg tagagcttac gaattttggg aagcattaga aaatcagcaa taagataatc
      301 tattgttaat ttaagctaat caacctacaa aatttgttta tttgtattaa aatataatct
      361 tagaacctca gtaacttagg tactcagcaa ctataaaaat gaaattaaga gccgaaaata
      421 ttgttaaaac ttacaaagga agaagtgttg ttaaaggcgt ttctgtagag gtaaaccaag
      481 gagaaattgt gggtttatta ggcccaaatg gtgccgggaa aactacttct ttttacatga
      541 ttgttggttt agttaaaccc aatagtggta aaatatacct tgacgatcta gaaattactg
      601 actttccaat gtacaaaaga gctcaaaatg gaattgggta tttagcccaa gaagcttccg
      661 tttttagaaa attaagtata gaagataata ttttaagcgt tttacaacta accaaacttt
      721 ctaaagctga acaagaagca aaaatggaaa gtttgattga tgaatttgga ttgcaacaca
      781 ttagaacgaa tagaggcgac ttattatccg gtggtgaacg ccgtagaact gaaattgcaa
      841 gagcattagc aactgaccca aaatttatct tattagatga gccgtttgcc ggagttgacc
      901 ctgtagcagt agaggacatt caacgcattg tcgcacaatt aaaaaataaa aacatcggca
      961 tactgattac cgaccataac gttcaagaaa cgttggcaat cacggacaaa acttatttaa
     1021 tgtttgaagg cggcatctta aaagccggta ttccggaaga acttgctgct gatgagatgg
     1081 taagaaaagt atatttagga cagaattttg aattacgtaa aaaaaagctg gaattttaag
     1141 tttacgtcat tgcaaaacgg aataattcaa catttttgga gttccattat aaatattgat
     1201 atcgacagga cctttgatgc ctggaatcgt agctttttct gtaaactgcc aaaaaagcca
     1261 atcatcttta atttgctcta caaaaaaatt ataattggca atccaaaaag agtagccttt
     1321 aaattctttt ttcaaaaaat ctttataata actttcgcct gaataaataa tcggtttaac
     1381 accataatgg ttttcaactt ttgttaacca tcgacgtaat ccaactttta aactatcgat
     1441 tgattgtgtt ttaggtaagt tttcaatatc taaaattggt ggaaaatctc cttttttaag
     1501 cgtaacagtt ttgatgaaat tatcggcttg tttaattgaa ttttcattgg gacgataata
     1561 gtgataggct ccacaaacaa atccgtattc ttttgctttc gaccaattat gtttaaattt
     1621 agcatcaatt ctatctttcc ccgcagttgc cctgataaaa gcaaattcta attggaaggt
     1681 atcttcaact gttttaactt cttcccaatg aatatcacct tggtattctg aaacatctaa
     1741 accaaagaca tttgaggaat gtttgcttaa tacttggaaa attcttgctc cttcaatttt
     1801 tgtagtatta gccgttttta attcgtgtct ggagcgaaaa ctaaaatagt aagccaatgc
     1861 ttctctataa tgatatcctg caaatccaat aaatagtaga aataaaagca atgctccaat
     1921 tgataaaaac tttcctgaaa aaggcgattt tttacggctg gattttcttt ttacagtagg
     1981 ttttctttta accggttttc tcatttatta gaaaataatg aaaattaaaa cgattattga
     2041 gccgttttat tttgaattac agaaagcaga acataaagta atattactaa tggcaagcct
     2101 agtatcttaa aactaaacaa tagaattagg gaaaacagta aaaaaagtac ttgtatcttg
     2161 tgcgtttccc atgaaaaatt cttcattttt aaagagaata gaggaatctc agcatttaag
     2221 atataagcac taaaagctga aatcgccatc aaaaagtaag ggttactcaa gaattctttt
     2281 agtcctaaaa aagtagtaga atcaataatt aaagggatgc ttaatataaa taatgcattg
     2341 gccggggttg gcaaaccaat aaaagaatta gtttgacggg tatcaatatt aaatttagcc
     2401 aaacgataac aagaacctaa tgttattaga aaacctgcca atggcaaaat atggttggtt
     2461 ggaaaaaaat catcaaatac tgaaatacct tcattttgat acttaaaaat caattgaaac
     2521 atcacaaatc cgggaacagc tccactggta accatatctg ctaaagaatc taattgaacc
     2581 cccaatggac tttgaacttt aaataaacgg gcaaaaaaac catcaaaaaa gtcaaaaaat
     2641 attcccaaac aaacaaagaa aaaagcaata tcaaaccgat ttccagcaac aaaaactaac
     2701 gctatacagc ctgataataa gtttaataat gtaataaaat tgggtatttt cgacttaata
     2761 ttcatattca atggtttgaa aacaaattta atacaataac tcacaaacaa gtgcgttttt
     2821 tacttgatat ttttatggga atgttttttt ctgttttatt tcatcaatag aaagaagcac
     2881 ctcactaaaa ttttctattt ttgagaaaaa attgtaaaac ccaaagggta tataaaaaat
     2941 ttgctttgaa aaaaaccttt atatgttttt tgctgttcat cacgtccttt tcttatagcc
     3001 aatccatcag aaaatattct aatgagttca tgaatattgg tgtagatgca gcggctttag
     3061 gaatgtctaa tgctgttaca gccaattcaa acgatgtgaa ttcaggctat tggaatccgg
     3121 caggattaat ccatttagaa gacaatcagc tatccttaat gcatgccagc tattttgcca
     3181 atatagccca atatgattat gctgcctttg ccatgccaat tgatgatcga agtgcttggg
     3241 gattttcatt aattcgattt ggagttgatg atattttaga tactactcaa cttattgaca
     3301 gcgaaggaaa tattgattat aaccgaatta atttattttc aactgctgat tatggagcaa
     3361 ctttttcata tgccagaagt cttcaagtgc cgggttggca atacggagta aatgcaaaaa
     3421 ttattagaag aattatagga aaatttgcta attcgtgggg ttttggaata gatgttggga
     3481 ttcaatttga acgtaatgac tggaaatttg ggttaatggt cagagacatc acaacaacct
     3541 ataatgtttg gaatattgat gaagagaaat tccaaagcat tgccgatgcg atacccggtg
     3601 aaaatcagga attgccggaa agtacagaaa ttacgcttcc aaaagctcaa tttggaattt
     3661 caagaaaatt cgaatttaat tatgattata ctttactgag ttcaataaat ttgaatatgc
     3721 agtttgctaa aaccaatgat attatttcaa ctgatgccgt tagtattgat cctgcatttg
     3781 gttttgagtt tgggtacacc gatttagtat ttttaagagc cggaattggg aattttcaaa
     3841 acatccaaca aattgacaat tcagaaaaag tgggatttca acctaatatt ggtttaggtt
     3901 ttaaatacaa aggaattcaa attgactatg cactaaccga tattggcgat caaagtgtcg
     3961 cattatattc aaatgttttc tcggtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnngatgt ggtctataac tttggtatgt ttgatttcag tacgcccaat ttttatttaa
     4141 aatttgtaaa aggcgatatg caatactatg ttgccaacag ttcatttgaa cattttatgt
     4201 attcctatcg aatggaaaac agaagtgttt ttgaacagga attaaattta acaacggaac
     4261 aaaaacaaaa actttttgat aatttaaatc aagctgtatt ttctgaagga cgcttttata
     4321 catataaatt tattgataaa aactgcacaa caatggtggt tgacaaaata aatgatgaat
     4381 tagggaaacc tgttattaaa aaagtgggca atatcgaaga aagttatcgt gatgtgctta
     4441 atccctattt agctaattta ttttatgaaa aattagggat caatattatt tttggccacc
     4501 gaaccgatga aaaagctgaa caattatttc ttccaattga attattaaaa agtttagagc
     4561 aacttgaatt taacaacaaa cctttagccg gtaaaacggt aacattattc caacaagatg
     4621 ttacattact ttcaaattca tggtggaata ctatttatac ttttctatta tttttagtat
     4681 tagttgtggt ttctaataga aattggatat acctgctttt tttattcctt ttaggtagtt
     4741 taggaatctt cttgtcatta gtgggatttt attctttaca tcgggaagta ttacaaaact
     4801 ataatgttct gctttttaat ccgtttttaa ttggattagt ttattgctat attaggaaga
     4861 atttgatttg gtttaaacgc atattttact ttgttttgac aagttttagc atttatttgc
     4921 tgtttatgct aaacaaaaca catttatttc aaatgctacc tattctactg acgatagtaa
     4981 taattctttt taaattgaga aaaagaatcc tttcaactca ataactactt attgccccct
     5041 aaaaaataag attgtactta aggttttaag cattatatta aaatctaaaa agatgcttcg
     5101 gtgtttgatg tagtacaaat catattgcaa tttgattaaa ctgtcatcaa tagaatctcc
     5161 ataagaataa tttacctgag cccaacctgt taatcccggt tttacaacat gacgcgtttg
     5221 ataaaaaggc ataatttcag caatttcatt gacaaaaaca ggtctttcag gtctcggacc
     5281 gataacagcc atatgtccta ataagatatt tataaactga ggaaactcat caattctgct
     5341 ttttcttaaa aatttcccaa acggagttat tctggcatca ttagtagtag caaaaacagc
     5401 tcctgctgat tctgcatttt taaccatcgt tctgaattta ataattctga actcttctcc
     5461 atttttacct acccgaattt gagaataaaa aagttttcct cgatttccta taaaattacc
     5521 tattaaaata aatggaacaa aaaacaaacc aagagtaatt cctattatcg ctattacgta
     5581 ttcaaatatt ctaacaaaaa gcaagtacaa ttgattttga ttacttcgac taaaaggaaa
     5641 atatcgataa aaatctctcg ctacgtattg aacaggaatt cgttgcgtca agttttcata
     5701 aacatgagta tattctctaa tgataaaacc attttcaagt agaagcaaca atttattata
     5761 taaatcaact gtaatttctt ccgttttttg agtagctata actatttcag aaattgaatt
     5821 cttagaaaca aatttttcta aaccggccac agaaataata gaaactgttt tatttagaat
     5881 agaaaaatct gaattttcat ctgtattaac taaggcaact atagtataat gcgggtctac
     5941 actttttaat cccaaaatca attcatcggc taattctttt tcacaaatta aaataacttt
     6001 cttttcaaaa cgatgtga
//
LOCUS       DAOTCW010000138         5980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_9822_length_5980_cov_52.618903, whole genome shotgun sequence.
ACCESSION   DAOTCW010000138 DAOTCW010000000
VERSION     DAOTCW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9822_length_5980_cov_52.618903"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1547
                     /gene="serS"
                     /locus_tag="P5335_09815"
     CDS             276..1547
                     /gene="serS"
                     /locus_tag="P5335_09815"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRZ75217.1"
                     /translation="MLQLTVIRENKEKVIKALAKRNLDAQSMVNNVLDLDEKRRTTQV
                     ELDNILAESNLLSKEIGEMMKSGEKAKAEIVKQKTVQLKEKSKELSEKLEVYTSSLVE
                     ELYKIPNTPADIVPEGKTPEDNLTVHQEGTIPTLHEGALPHWELVKKYDIIDFELGVK
                     ITGAGFPVYKGKGAKLQRALITYFLDKNTEAGYLEYQVPHLVNEASGFGTGQLPDKEG
                     QMYHDATDNLYLIPTAEVPVTNLFRDVILNENELPIKCTGYTPCFRREAGSYGAHVRG
                     LNRLHQFDKVEIVRIEHPDKSYEALDGMVEHVKTLLQELKLPYRILRLCGGDMGFTSA
                     LTYDFEVFSTAQDRWLEISSVSNFETFQANRLKLRFKDKEGKNQLTHTLNGSSLALPR
                     VLAGILENYQTPEGIVIPEVLRPYTGFDIIN"
     gene            1623..3401
                     /locus_tag="P5335_09820"
     CDS             1623..3401
                     /locus_tag="P5335_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRZ75218.1"
                     /translation="MQKIILFLVFLYSTIVLSQNDQLAQNYYDRGEFEKALLSYEELL
                     KVQQGNSIYFQRIIDCHQQLLQFDKAEKAIQERLDKYRMASMYVELGYNFQLQKNQVK
                     AELNYKEAIEKIKENTSNVYSVAGAFEKKVLLDYALQSYQLAISIDPRNKFNYQMALL
                     YGQMGKPDLMIENYLEEMYENPQNSVMVQNQLSRFMNEDADESFNAMLRKALLVQVQK
                     NQDVFWNQMLSWFFVQQKEYGKAFIQEKAIYKREPESFSNIVNLAELAIEENENEIAA
                     EILTFILENTQDLDLQIYAHHYLMSIRISKAKNTDYAAINAEFQNLMKQYGVNPYTLS
                     LQRLQAEFLAFKMKMPDAGKAILQKAMELPLNKFQVAEAKMELADILLYEEKFNQALL
                     YYTQIEEDLKNDVLGHEASLKAAKTSYYKADFNWALTQLKVLKSASTQLIANDALELF
                     LLINDNTVADSTQIALKKFAKADFLLYQNKNQEALVQFQQIVKEHKGDVIEDVTLLRI
                     GKTYEQLADFTTALSFYQQIIDNFPESIYRDEALFFSAELYNKQLNQPEKAKTLYEAV
                     LFNHQDSIYYVEARKKFRQLRGDSNL"
     gene            3471..3800
                     /locus_tag="P5335_09825"
     CDS             3471..3800
                     /locus_tag="P5335_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HRZ75219.1"
                     /translation="MILYNVTINIDLSVHDQWMKWMQTKHINDVLATGKFTSARMVKV
                     LVEEEGSVTYSIQYTTESKEKLERYYQEDAPRLRQEGLALFADKMLAFRTELQVMSEH
                     FPKSALQ"
     gene            3841..4620
                     /gene="rsmA"
                     /locus_tag="P5335_09830"
     CDS             3841..4620
                     /gene="rsmA"
                     /locus_tag="P5335_09830"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRZ75220.1"
                     /translation="MEKVKAKKHLGQHFLNDENIAQKIADSLTLVGYQTVLEIGPGMG
                     VLTKYLLEKPITTFVIEIDTESVTYLQNHFPKLDGKIIAKDFLKYNLSEVFGTEPFAI
                     IGNFPYNISTQIVFKTLELRHQIPEFSGMFQKEVAERICEKKGTKAYGILSVLTQAFY
                     EAEYLFTVSEHVFIPPPKVKSGVLRLRRKENYQLPCDEKLFFSVVKTAFNQRRKTLRN
                     SLKTYQLSDNLKEDSIFDLRPEQLSVEQFIELTQKIAADGV"
     gene            4610..5962
                     /gene="mgtE"
                     /locus_tag="P5335_09835"
     CDS             4610..5962
                     /gene="mgtE"
                     /locus_tag="P5335_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRZ75221.1"
                     /translation="MEFKISREFLTQIEQLIEGNKEQELHDLLHEIHYADIAEIMEEL
                     DHVEASYIFKILDSETTSEVLLELDEEVREKILRDLSPKEIAEELDELSTDDAADIIA
                     ELPQARKEQVISELEDVEHAKDIVDLLRYDEDSAGGLMGKELVQVNENWNVLTCVKEM
                     RIQAENVSRVHSIYVVDDENRLKGRLSLKDLLTTSTKTPISDIYIPKVDYVKVDTKDV
                     EVARIMQKYDLEAIPVVDELGRLVGRITIDDIIDVIKEEADKDYQLAAGISQDVEADD
                     SIIQLTKARLPWLVLALLGGFIAVKISGQFGGAMKEFGVLFFFTPLIAAMAGNVGVQS
                     SAIIVQGLANNTLGGTIWQRLFKEMKLSLFNGFILSIILLLGSHFLLGVEYIIGITVS
                     ISLITVIVIASLIGTFVPITLNKYGIDPALATGPFITTSNDIFGILIYFSIAKMILGF
                     "
BASE COUNT         2095 a          929 c         1129 g         1827 t
ORIGIN      
        1 gaagatttta attctgcttc agcaatttca tttacttcct ttaaaaagtc ttgagcgaat
       61 aaagggttga tgcaagcaaa cagtataatt agtaggtatt ttttcatttt gtttggaaat
      121 tgtaggccaa atgtagaaaa atttattgat ttataacttt ttgatgattt ttttagcatt
      181 tttttaaacc ttgttttagt ctttatcatt tttaaaatca gcatttaccg ttacaaaaca
      241 tttccgtaca tttgtgctcg caaaaaacgc atattatgtt acaactgaca gtcattcgag
      301 agaataaaga aaaagtaatc aaagctttgg ctaaaagaaa tttggatgcc caaagtatgg
      361 ttaataatgt cttagattta gacgaaaaaa gaagaactac acaagtagaa ttagataata
      421 tcttggctga atccaatttg ctttcgaaag aaattggcga aatgatgaaa tcgggcgaaa
      481 aagcaaaagc cgaaattgtc aaacaaaaaa cagttcagtt aaaagaaaaa agcaaagaat
      541 tatcagaaaa actagaggtt tatacttcgt cacttgtaga agaattatat aaaataccaa
      601 atactcctgc cgatattgtt ccggaaggta aaacacccga agataactta acggttcatc
      661 aggaaggtac tattccaact ttacacgaag gagcattacc acattgggaa ttggtaaaaa
      721 aatatgacat tatcgatttt gaattaggag taaaaatcac cggtgccggt tttccggttt
      781 acaaagggaa aggtgcaaaa ctgcaacggg cattaattac ctatttctta gataaaaaca
      841 cagaagccgg ttatttggaa taccaagttc ctcatttagt gaacgaagct tccggtttcg
      901 gaactggaca attacccgac aaagaagggc aaatgtatca cgacgcgacc gataatttat
      961 acttaattcc aaccgctgag gttccggtaa caaatctatt tcgggatgtg attttaaacg
     1021 aaaatgagtt gccaattaaa tgtaccggtt acacgccttg ttttagaaga gaagccggtt
     1081 cttatggtgc tcatgttcgt ggtttaaatc gtttacacca atttgataaa gtagaaattg
     1141 tccgcatcga acatccggat aaatcatatg aagccttaga tggcatggta gaacatgtga
     1201 aaactttatt acaggaattg aaattaccgt atcgaatttt gcgtttgtgt ggaggagata
     1261 tgggttttac ctctgcttta acgtatgatt ttgaggtttt ttcaaccgct caggaccgtt
     1321 ggttggaaat tagttctgtt tctaattttg agacattcca agccaatcgt ttgaaattgc
     1381 gttttaaaga taaagagggt aaaaaccaat taacccatac cttgaatgga agttctttgg
     1441 ctttacctag agtattggcc ggaattcttg aaaattatca aacaccggaa ggaattgtta
     1501 ttccggaagt gttgcgtccc tacaccggat ttgacattat caactaaaaa agttaatctt
     1561 tatacaaacc atgtactaat tagcgaaaaa aagctatttt agtacattgt tttttatgtc
     1621 ctatgcagaa aattatcctc tttttggtgt ttttatattc aacgattgtt ttatctcaaa
     1681 atgatcagtt ggcacaaaac tattatgacc gaggcgaatt tgaaaaagca ttattgagct
     1741 atgaagagtt gctgaaagta caacaaggga acagtattta ttttcaacgg attatagatt
     1801 gccatcaaca actacttcaa tttgataaag cagaaaaggc tattcaagaa cgtcttgaca
     1861 aatatagaat ggccagtatg tatgtagaat taggctataa ttttcaattg caaaaaaatc
     1921 aggtaaaagc cgagttgaat tacaaagaag cgattgaaaa aattaaggaa aacacctcaa
     1981 atgtttatag tgtagctggt gcatttgaaa agaaagtgtt gttagattac gcattacaat
     2041 cctatcaatt ggcaatttct atagacccca gaaataaatt caattatcaa atggccttgt
     2101 tgtacggaca aatggggaaa ccggatttaa tgattgaaaa ttacctcgag gaaatgtatg
     2161 agaacccaca aaacagtgtt atggttcaaa atcagttgtc gcggtttatg aatgaggatg
     2221 cagatgaatc gtttaatgca atgctaagaa aagcattatt ggttcaagtg caaaaaaacc
     2281 aggatgtttt ttggaatcaa atgttgagtt ggttttttgt tcaacaaaaa gaatatggta
     2341 aagcatttat tcaggaaaaa gcaatttata aaagagaacc ggaatctttt tcgaatattg
     2401 taaaccttgc tgagttggcc attgaagaaa atgaaaatga aatcgctgct gaaattttga
     2461 cttttatttt agaaaacacg caggatttag atttgcaaat atatgctcac cactatttaa
     2521 tgagcatacg aattagtaaa gctaaaaata cagattatgc tgcaatcaac gctgagtttc
     2581 aaaatttaat gaaacaatat ggtgtcaatc catataccct ttctttgcaa agactgcaag
     2641 cagaatttct cgctttcaaa atgaaaatgc ctgatgccgg taaagccatt ttgcaaaagg
     2701 caatggaatt gccattgaat aaattccaag tggcggaagc taaaatggaa ttagcggata
     2761 ttttgttata tgaagaaaaa tttaatcaag ctttattgta ttacactcaa atagaggaag
     2821 atttaaaaaa tgacgtactt ggtcatgaag ccagtttaaa agcagcaaaa acaagttatt
     2881 ataaagccga ctttaattgg gccttgacac aactaaaagt acttaaatca gcttctactc
     2941 aattgatagc gaatgatgcc ttagaattgt tccttttaat aaacgacaat actgtggccg
     3001 attctaccca aattgcatta aagaagttcg cgaaagctga ttttctttta tatcaaaata
     3061 aaaatcagga agcattagtt caatttcagc aaatagtaaa agaacacaaa ggagatgtta
     3121 ttgaagatgt tactttgtta cgaataggta aaacctatga acaattagcc gatttcacca
     3181 ctgctttgtc gttttaccag caaattattg ataatttccc ggagagtatt tatagagatg
     3241 aagctttgtt tttttcagcg gaactatata acaaacaact taatcaacct gaaaaagcca
     3301 aaacattgta cgaagccgta ctttttaatc accaagacag tatttattat gttgaagctc
     3361 gaaagaaatt tcgtcaactt cgtggtgact ctaatttgta attcaaagaa taaaactttt
     3421 agtccaattt tgctccattc tgcttagatt ggtgctgata aaaaataatt atgattctat
     3481 acaacgtaac catcaacatc gatttaagcg ttcatgacca atggatgaaa tggatgcaaa
     3541 caaaacacat taatgatgtt ttagcaaccg gaaaattcac ctcggcccga atggttaaag
     3601 tattggtaga agaagaagga agtgttacct attcgattca atatacaacc gagagcaaag
     3661 aaaaattgga acgctattat caagaagacg ctcctcgttt aagacaagaa ggccttgctc
     3721 tatttgcgga taaaatgtta gctttccgaa cggaattgca agtgatgagt gaacattttc
     3781 caaaatctgc attgcagtaa aataacattc aaagataatt cagcaactcc gccaataaaa
     3841 atggaaaaag taaaagcaaa aaaacacctt gggcaacatt ttttgaatga tgaaaatatt
     3901 gctcaaaaaa tagccgattc attgactttg gtggggtatc aaactgtctt ggaaattggc
     3961 cctgggatgg gtgtgctgac caaatattta ctggaaaaac ctattacaac ttttgtcatc
     4021 gaaatcgata cagaatcagt aacctatttg cagaatcatt ttcctaaact tgatggaaaa
     4081 attatagcta aagatttttt aaaatataac ctttcagaag tatttggaac ggaacctttt
     4141 gccattatcg gaaattttcc ttacaacatt tctactcaga ttgtttttaa aacattagaa
     4201 cttcggcatc aaattcctga attttcaggg atgtttcaaa aagaagtggc tgaacgaatt
     4261 tgtgaaaaaa aaggaacaaa ggcctacgga atactatctg ttttaaccca agcgttttat
     4321 gaagccgagt acctttttac ggtttctgaa catgttttta ttccgccccc aaaagtaaaa
     4381 tcaggggtat tgcgattgcg tagaaaagaa aattatcaat taccgtgtga cgaaaaattg
     4441 tttttttccg tcgtgaaaac ggcctttaac caacgaagaa aaacacttcg taacagttta
     4501 aaaacatacc aattgagtga taatttaaaa gaagatagta tctttgacct cagaccggaa
     4561 caactttcag tcgaacaatt tattgaactc acgcaaaaaa tagccgccga tggagtttaa
     4621 aatcagcaga gaatttctca cgcaaattga gcaattaatt gaaggaaaca aggagcagga
     4681 attacacgat ttgctccatg aaatccatta tgctgatatt gctgaaatca tggaagaact
     4741 tgaccatgta gaagcaagtt atatttttaa aattcttgac agtgaaacaa cctcagaagt
     4801 tttattagaa cttgatgaag aagttcgtga gaaaatatta agggatttat caccgaaaga
     4861 aattgccgaa gaattagatg aactttccac ggatgatgcg gcagatatta ttgcggaact
     4921 tccacaagca agaaaagaac aagtaatttc tgaattagaa gacgttgaac acgccaaaga
     4981 cattgtcgat ttattgcgat atgatgaaga ttctgccggt ggtttgatgg gaaaagagtt
     5041 ggtgcaagta aatgaaaatt ggaatgtgct tacctgtgtg aaagaaatgc ggattcaagc
     5101 tgaaaatgtt tcccgtgttc attctattta tgttgttgat gatgaaaaca ggttaaaagg
     5161 aagattgtct ttgaaagatt tattaaccac atcgaccaaa acccccatta gcgatattta
     5221 tattccaaaa gtagattatg taaaagtaga tacaaaggat gttgaagttg cccgtatcat
     5281 gcaaaaatat gacttggaag ccattccggt tgttgatgaa ttagggcgat tggttggacg
     5341 aatcaccatt gacgatatta tagatgttat caaagaagaa gctgataaag attatcagtt
     5401 agctgccggt atttctcagg acgttgaagc agatgacagt attatccaac ttactaaagc
     5461 acggttacct tggttagtct tagccttatt aggaggattt atagccgtga aaatttccgg
     5521 acaatttgga ggagccatga aagaatttgg tgtattgttc ttttttacac ctttaattgc
     5581 cgcgatggcc ggaaatgtcg gggtacagtc ttccgcaatt attgttcagg gtttagctaa
     5641 taatacgctg ggaggaacca tttggcaacg tttgtttaaa gagatgaaat tgagtttgtt
     5701 taatggtttt attttatcca taattctttt attagggagt cattttttgc ttggagttga
     5761 atatataatc ggcataacgg tttccatatc gttaattacg gttattgtta tcgcgtctct
     5821 cattgggaca tttgtgccca tcactttaaa taaatatggc attgaccccg ctttggcaac
     5881 aggaccattt attactacaa gcaatgatat ttttggaatt ttaatttatt tttccattgc
     5941 taaaatgatt ttaggattct aaaaaggtat taatagttac
//
LOCUS       DAOTCW010000139         5960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_9872_length_5960_cov_40.119221, whole genome shotgun sequence.
ACCESSION   DAOTCW010000139 DAOTCW010000000
VERSION     DAOTCW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9872_length_5960_cov_40.119221"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..867)
                     /locus_tag="P5335_09840"
     CDS             complement(1..867)
                     /locus_tag="P5335_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRZ75222.1"
                     /translation="MIQSSELILNPDGSVYHINLKPGQIANDIIFVGDQNRVEKITKH
                     FDSIEFSTQKREFKTQTGTYKGKRLTVMSTGIGPDNIDIVMNELDALVNIDLETRTVK
                     DTLTSLNIIRFGTSGSLQASIPVDSIVMSQYGLGLDNMLRSYLIDSIAEKEMEEAFIA
                     QTNWDLKKGRPYVIKGSETLAARLDSPHIYKGFTGTAGGFYGPQGRVLRLGIQDPELN
                     LKMDAFNFKGIQMTNLEMETGAIYGLGKLLGHECLSLNAIIANRATGIFSEDPYKAVD
                     GLIEYALDKLVE"
     gene            complement(982..1317)
                     /locus_tag="P5335_09845"
     CDS             complement(982..1317)
                     /locus_tag="P5335_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HRZ75223.1"
                     /translation="MNDLSEQLKKLFPDHQPSNEPEQVDETPHELYVQKEPIICQYEK
                     RKGKPTTIISGYEGTEEDFKILAKEIKSKLAVGGSFKDDEIIIQGDYRDKIMKILQEK
                     GFKTKRVGG"
     gene            complement(1454..2404)
                     /locus_tag="P5335_09850"
     CDS             complement(1454..2404)
                     /locus_tag="P5335_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HRZ75224.1"
                     /translation="MQKIPSVDLRDFLSDDPVRKQKFVNEIGKAYSDIGFVALKGHFL
                     DDRLVGELYGEVRNFFSLPVETKLKYEIPGIGGQRGYVSFGKEHAKGRSAGDLKEFWH
                     FGQFVEKDSKYADEYPDNVEVAELPKFNSTGKEAYQMLEKTGIYVLRALALYLGLDEF
                     YFDNYIKEGNSILRPIHYPPITDEPKDAVRAAAHGDINLITLLMGAQGKGLQVQNHNG
                     DWIDAMAEPDELMINVGDMLSRHTNNKLKSTIHQVVNPPRELWGNSRYSIPFFMHPVS
                     DMRLDVLEDCIDENHPKLYEDISAGEFLHERLVDLGLIKK"
     gene            2593..4569
                     /locus_tag="P5335_09855"
     CDS             2593..4569
                     /locus_tag="P5335_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HRZ75225.1"
                     /translation="MIFDRRDLSDTTLLDLYIRLLKPRLIEEKMLILLRQGKVSKWFS
                     GIGQEAISVGITSALDKDEYILPMHRNLGVFTTREIPLHRLFAQWQGKKSGFTKGRDR
                     SFHFGTQEFKIIGMISHLGPQLGVADGIALANKLKKNGKITAVFTGEGATSEGDFHEA
                     LNIAAVWDLPVLFVIENNGYGLSTPTNEQYRCTNLADKGIGYGMESHIIDGNNILAVY
                     TKVAALAASLRENPRPVLLEFQTFRMRGHEEASGTKYVPQELMDEWAQKDPIENYRNY
                     LKDIGLLTNEYDETIRSAYKQEIDENIHIAFDEPDIEADLSEELNDVYKPYDFEQFNH
                     NDNVEKIRVIDAITQSLKQSMERHPNLVIMGQDIAEYGGAFKVTDGFVNLFGKERVRN
                     TPICESAVVSAGMGLSINGYKAIVEMQFADFVSTGFNPIVNYLAKVHYRWQESADVVV
                     RMPCGGGSQAGPFHSQTNEAWFTKTPGLKVVYPAFPYDAKGLLNTAINDPNPVMYFEH
                     KGLYRSIYQEVPTDYYTIPFGKAALLKEGSQVTIISFGAGVHWALETLALHPEISADL
                     VDLRTLQPLDTEAIYASVKKTNKCIILQEDSLFGGIASDISAMIMENCFEQLDGPVRR
                     VGSLESPIPFVKALEDQYMGKGRFEKELLELVAY"
     gene            complement(4992..5393)
                     /locus_tag="P5335_09860"
     CDS             complement(4992..5393)
                     /locus_tag="P5335_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRZ75226.1"
                     /translation="MRTNFDSLEAYWQAQPKETLLSLVNLQQTINDFVADSEWYISYQ
                     IPTVRYQGKPLVAIAGYQHHCSLYVMSKAIVSLLEQELAGFDYSGTTIRFQPTQSLPN
                     SLVEKIIALRKEEIALVLAAKRYRKKGSSEK"
     gene            5523..>5960
                     /locus_tag="P5335_09865"
     CDS             5523..>5960
                     /locus_tag="P5335_09865"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013998504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRZ75227.1"
                     /translation="MGDFNDQLCLCDWRYRKMRQEVDNRIIKGLNTECIEQSTPLLVE
                     TQKQLAAYFDKKLTVFDLPLLFIGTDFQQTVWKALQQIQYGKTKTYLDLAKQLNNPKA
                     IRAVASANGANAISIIVPCHRIIGSNQELVGYAGGLSAKKKLLE"
BASE COUNT         1897 a         1225 c         1061 g         1777 t
ORIGIN      
        1 ttactccacc aatttatcca acgcatattc aatcaaacca tccaccgctt tatagggatc
       61 ttcactaaat attccggttg cacggttagc gataatggca tttaacgaca aacattcatg
      121 ccctaataat ttacctaaac cataaatggc tccggtttcc atctctaaat ttgtcatttg
      181 aataccttta aaattaaaag catccatttt taaatttaat tcagggtctt gaattcctaa
      241 tcgcaatact cttccttgag gaccgtaaaa ccctccggct gttccggtaa atcctttata
      301 aatatgaggg ctgtctaatc tggctgctaa cgtttcactt cctttgatta cgtagggacg
      361 gccttttttt aaatcccaat tcgtttgagc aataaaggct tcttccatct ctttttcagc
      421 aatagaatct attaaatagg agcgaagcat attgtctaac cctaatccat actgactcat
      481 cacaatacta tccacaggga tagaggcttg taaagagccc gaagttccaa atcgaataat
      541 atttaaagaa gttaaagtgt cttttacggt tcgcgtttcc aaatcgatat tgaccaatgc
      601 atctaattca ttcatcacga tgtctatatt gtcaggacca attcccgttg acatcacagt
      661 caatcgtttg cctttatagg taccggtttg cgttttgaat tctctttttt gagttgaaaa
      721 ttcaatcgaa tcaaaatgtt ttgtgatttt ttctactctg ttttgatcgc caacaaaaat
      781 aatatcgttg gctatttgac ccggttttaa gttaatatgg taaacactac catcggggtt
      841 taaaattaat tctgaagatt gtatcatagt ttgtttaaat agtttaaggg gtttaatagt
      901 ttagaggttt aaaattgttt aagcgttaaa aggaaaaact ttaaacaatt ttaaacaaca
      961 ttgaacatta ttaaaccata gttaccctcc tacacgtttg gttttaaatc ctttctcttg
     1021 gagtattttc attattttat cacggtaatc gccttgtatg attatttcat catctttgaa
     1081 gctaccacca acggctaatt tactcttaat ttctttagcc aaaattttaa aatcttcctc
     1141 tgtgccttca taacctgaaa taatggtggt aggttttcct tttcgctttt catattgaca
     1201 aattattggt tctttctgaa catataattc gtgcggagtt tcatcaacct gctccggttc
     1261 attcgaaggt tggtggtcag ggaatagttt tttgagttgt tcgcttaagt cattcatttt
     1321 ttaagatact aagttgctaa gttactgaga ctaaacgatt ataaattctt taaatccctt
     1381 aacctcataa cctttaaaac aaaaagacat caagctaaaa ctcaatgtct ttctttttat
     1441 gagtttttat aatttacttc ttaatcaatc ccaaatcaac caatcgttca tgcaaaaatt
     1501 ctcctgcaga aatatcttca tataatttcg gatgattttc atcaatacaa tcttctaata
     1561 catctaatcg catgtcactc acaggatgca tgaaaaaagg aatggaatac cttgaatttc
     1621 cccataattc cctcggtgga ttcaccactt gatgaatggt tgattttaat ttattattcg
     1681 tgtgtctcga caacatgtcg cctacattaa tcatcaattc atccggttca gccatcgcat
     1741 caatccaatc tccgttgtgg ttttgaactt gcaatccttt tccctgagct cccatcaaaa
     1801 gcgtaatcaa gttaatatca ccatgggctg ctgctcgaac agcatctttt ggctcatccg
     1861 taataggcgg atagtgaatc ggtcttaaaa tactattccc ttctttaata taattatcaa
     1921 aataaaactc gtctaatcct aagtataaag ccaaggctct caacacataa atacccgttt
     1981 tttcaagcat ttgatacgct tcttttccgg ttgaattgaa ttttggtaat tcagctactt
     2041 ctacgttatc aggatattca tccgcatact tggaatcttt ctccacaaat tgaccaaagt
     2101 gccaaaactc ttttaaatca ccggcagaac gtcctttagc gtgttcttta ccaaacgata
     2161 catagccacg ttgtccacca ataccgggaa tttcatactt taatttggtc tctaccggaa
     2221 gagagaaaaa attacgcact tcaccatata attcgcccac caaacggtcg tctaaaaagt
     2281 gacctttcaa ggctacaaac cctatatctg aataagcttt tccgatttca tttacaaatt
     2341 tttgtttgcg taccgggtca tccgatagga aatcacgcaa gtcaacacta ggaatttttt
     2401 gcatactaaa tgattatttg ttaataactc aaagtgagtt gaatgcttgt aaatacaaat
     2461 gtaaataaat atttctatct gcaaaattga aagttatgaa catcactgaa aacctctttt
     2521 aaaaaagtat aacaatctat ttaaaatttg ttattttttg tacttttggg agcgtttaaa
     2581 aatagcgata ctatgatttt tgatagaaga gacctttccg acacaacctt acttgattta
     2641 tatattcgtt tactaaaacc ccgtttgatc gaagaaaaga tgctcattct gcttcgtcag
     2701 ggaaaagtct ctaaatggtt ctccggcatt ggtcaggaag ccatttcggt aggcatcact
     2761 tctgcgttag acaaagacga atacatcctt cccatgcatc gtaatttggg tgttttcacc
     2821 acgcgggaaa ttcccctgca ccgattattt gcccaatggc aaggcaagaa aagcggtttc
     2881 accaaaggaa gagaccgttc tttccatttc ggtacgcaag agttcaaaat cattggaatg
     2941 atatcgcatt taggcccaca attaggcgtg gccgatggta tagccttagc caataagtta
     3001 aagaaaaacg gaaaaataac tgccgtattt accggtgaag gagcaaccag cgaaggcgat
     3061 ttccacgagg ctttaaacat tgccgccgtg tgggacttac ccgtattgtt tgtcatagaa
     3121 aataatggat atggtttatc aacaccaaca aatgaacaat accgttgtac caatttagca
     3181 gacaaaggaa ttggctacgg catggaaagc catattatcg acgggaataa tattttagcc
     3241 gtttacacca aggtcgcggc cttagctgct tccttacgag aaaatccacg acctgttttg
     3301 ctggagtttc aaaccttcag aatgcgtggg catgaagagg cgagtggcac caaatatgtt
     3361 ccgcaagaac taatggacga atgggctcaa aaagacccca tagagaatta ccgcaattat
     3421 ttaaaagaca tcggtctgct caccaacgaa tatgatgaaa caattcgatc ggcatataaa
     3481 caagaaatag acgaaaacat ccacatcgct tttgatgaac cggacattga agctgattta
     3541 agcgaagaat taaatgatgt gtataaaccc tatgattttg aacagtttaa tcataatgac
     3601 aacgtagaga agattcgcgt catcgatgcc atcacacaaa gcttaaaaca atcgatggaa
     3661 cgtcatccca acttggtcat catggggcaa gacatcgccg aatacggtgg agctttcaaa
     3721 gtcaccgacg gttttgtcaa cctgtttgga aaggagcgag tacgcaacac acctatctgc
     3781 gaaagtgcgg tggtttcggc cggaatgggc ttgtccatca acggttataa agccattgtc
     3841 gagatgcagt ttgctgattt cgtatcaaca ggattcaatc ccatcgtgaa ttatttagca
     3901 aaggtgcatt accgctggca ggaaagtgcc gatgtagtcg tgcgaatgcc ttgtggaggt
     3961 ggttctcaag caggaccgtt tcactcacaa accaacgaag cttggttcac caaaacaccc
     4021 ggattaaaag tagtctatcc cgcatttcct tatgatgcaa aaggactatt aaacacggcc
     4081 attaacgacc cgaatccggt catgtatttt gaacacaagg gcctttatcg tagcatctat
     4141 caagaagttc ccactgacta ttacaccatt ccgtttggta aagccgcttt attaaaagag
     4201 ggctctcagg tcactatcat ctcgttcgga gcaggggtgc attgggcttt ggaaacactg
     4261 gctcttcatc ccgagataag tgccgattta gtagatttaa gaaccttaca acccttagat
     4321 accgaagcaa tttatgcttc cgtcaagaaa acaaacaaat gcatcatcct gcaggaagac
     4381 tctttgtttg gcggcatagc cagcgatata tcagcaatga tcatggaaaa ttgctttgaa
     4441 cagttagatg gacctgttcg ccgggtaggc agtttagaat cgcccatccc gtttgtaaaa
     4501 gcgttagaag accaatacat gggcaaaggc cgattcgaaa aggaattgtt ggagttggtg
     4561 gcgtattaga ataaaggagg taccttttta aatgagttct aaaacaccgt agtcaatcaa
     4621 attaaaattt gcacagaaac aaacgctata acaatattta taaaagcaag tgaagcatct
     4681 gtcgaaacaa cttcataaag tttgatgtgc ttaagctttt tgatgaaaaa atcggtgaga
     4741 aagatactgt tgatgatcga aacaatcaga ataacaaaaa aacagataac acccagaaca
     4801 ttcgattgtg taaaatagta taaacccatt gatatcacta agataactaa ggtgataaga
     4861 gcaactttct tttccatagc aagccttttt acaaagtata aagataaaca ggataaaaga
     4921 atagcttgga aacttaacgt tattagtttt ttaaaagaga gttaactatt gattgttaag
     4981 ttactattag gttatttttc actactgcct tttttacgat atcgtttggc ggctaaaaca
     5041 agagctattt cctcttttcg caaagcaatg attttttcaa ctaaactgtt gggcaacgat
     5101 tgagtaggct gaaaacgaat agtcgtaccc gaataatcaa acccggcaag ctcctgttcc
     5161 agtaaagaaa caatcgcctt gctcatcaca tacaaactgc aatgatgctg ataccccgca
     5221 atagcaacca atggctttcc ttgataccga accgttggaa tttgataact gatataccat
     5281 tcactatctg caacaaaatc attaatagtc tgttgcaggt taactaacga aagaagagtt
     5341 tccttcggtt gagcttgcca ataggcttct aaagagtcaa aatttgttct catggtcagt
     5401 aataaaaaga aatagaatca acaaagttat aaaattgtat ctttgaatta catccaaatg
     5461 tgccaatgat gaatttacaa ctcaacacca tcctatacaa atctcccgtt ggcgaactga
     5521 tcatgggcga cttcaatgac caattatgtc tatgcgattg gcgttaccgc aaaatgcgtc
     5581 aggaagtaga caaccgcatt ataaaaggac taaacacgga gtgtatagaa caatccaccc
     5641 ccttattagt agaaacacaa aaacaactgg ccgcttattt cgacaagaag ttaaccgttt
     5701 ttgatttgcc cctcttgttc ataggaactg attttcaaca aaccgtttgg aaagccctgc
     5761 aacaaattca atacggcaaa acaaaaacct acctcgactt agcgaaacaa ctaaacaacc
     5821 ccaaagcaat acgggcagta gcctccgcca atggagcaaa cgccatttcc atcatcgtgc
     5881 cgtgtcatcg aatcataggc agcaaccaag aattagtagg ctatgccggc ggactgtcag
     5941 ccaaaaagaa gttgctggag
//
LOCUS       DAOTCW010000140         5929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_9950_length_5929_cov_40.567075, whole genome shotgun sequence.
ACCESSION   DAOTCW010000140 DAOTCW010000000
VERSION     DAOTCW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9950_length_5929_cov_40.567075"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2720)
                     /locus_tag="P5335_09870"
     CDS             complement(<1..2720)
                     /locus_tag="P5335_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRZ75228.1"
                     /translation="MNKITKIAISFFFLSMFGVGFSQHQHNLFVELYPEQKTLLIKQE
                     LIYHNTSENTLYKIVLNDWNNSFSEKNTPLAKRFSDEHYRGFHLANFKDRGSTNNLSI
                     LDSEKRIVDWCRPDKHPDIVEVLLSKPLKPGEKLILNLLYYVKIPNNKFTHYGFEIGG
                     GYSLKNWFLTPARIENNNFATYSNLNLDDIANAPFNIKMSLKMSKSLKLSSDLNVGIP
                     EIDLSNQTYFLEGQNRTNFSLFIETSQTFSSYKNDIVEVVTNLNDSKVDVIQKAIVID
                     RIVNFVDQNLGSYPHTKITVSQTDYDRNPFYGLNQLPSFISPFPNDLLFELKFLKTYI
                     NTFLEKSLSLDPRKDNWIYDGIQVYIMMNYMDENYPELKMMGNISKLKILKGYHLMDI
                     GFNEQYSYFYLLMARKNLDQAIGDLKDTSLKFNEQIAGKYRAGLSLKYLDSYLGDSIV
                     STGIKQFYTKNHKQQTNRTDFEKELKIKTDKNLNWFFETVIDSRDLIDYKFHNVTKTN
                     DSITATIHNKTGTAVPFPVYGIKDKKVVFKEWFEGFSTDSTITLPNNQADKIVINYNN
                     EVPEFNLRNNWRSVSNFHITNKPFKFVFFQDLEDPKYNQILYVPILNYNLYDGLMPGM
                     RFYNKTILNKPFIYDINPVWGVKSKTFVGSFSFLYTQNNRKSDWYQTIYSMSGSRFHY
                     APDAEFYKFNPMLIFRRRESDLRDNHKEQLLFRYVMIDRQQTDFIDTESTDEYQDYSI
                     FNVKYLNVRTEVLSHYSFKGDLQIDRTFGKASFEVDFRKLYDNNRQFNIRWYAGSFLY
                     QNTDTDYYDFGISQVNDYLFDYNLYGRSETSGFFSQQYVVGEGAFKSQLDTKLSNKWL
                     TTLNTSYSIWNWIELYGDIGLIKNENISPEFIYESGIRLNLLTDYFEIYLP"
     gene            2841..>3682
                     /locus_tag="P5335_09875"
     CDS             2841..>3682
                     /locus_tag="P5335_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HRZ75229.1"
                     /translation="MTKRVLLWVGLLMSTCVFSQVVINEVDADTPGLDVKEFIELKSQ
                     TPFFSLDGYIIVMYNGSTSGTGTLSYYTINLAGLITDGNGIILLGNSQVNPAPSLLFP
                     QNSVQNGPDAIAIYQDIVDNFPIDTPATALNLIDALVYGNGVTQATELQTILGVSTQP
                     NENVNSLAASQSIQRKNDGTYEVKTPTPRTNNDGSGIVYNGITLATSVSVLNEGENIA
                     ITFTTDTPVTTDLNFTLNLTNGSFTNADFTGNLSISIPSGTSSGSTTIQVIDDGLNDG
                     DETMS"
     assembly_gap    3683..3782
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3783..4673
                     /locus_tag="P5335_09880"
     CDS             <3783..4673
                     /locus_tag="P5335_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRZ75230.1"
                     /translation="HAHNYGDIVDILKTADQNPANNNEVWLMYVEQPRSKIDYQLGPS
                     TIGVWNREHIWPQSRGGFADGTSSFADGINIWLPTNADDILSGHADAHHIRAEDGQEN
                     SSRSNRDYGTDYNGPTGNLGTWKGDVARSLFYMAVRYNGLTLVNGNPADNISGQIGDL
                     ASLLTWNSTDPADDFEMNRNNYIYTWQVNRNPFIDYPNLADYIWGENYGQQWFPTLSQ
                     PKFDEANVRVYPIPTRDEITISGVESFAKVEIYAINGQQVLSKEIEGFTQLKLNLPSG
                     MYLMKIQTENQTITKKIIIK"
     gene            complement(4761..5486)
                     /locus_tag="P5335_09885"
     CDS             complement(4761..5486)
                     /locus_tag="P5335_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRZ75231.1"
                     /translation="MSVDQYESEEHRIQEKFKQKTWNEIRTNDSWAIFKIMSEFVNGY
                     ESMSRIGPCVSIFGSARTKPGEKYYELAENIAYKISKAGYGVITGGGPGIMEAGNKGA
                     HRGEGTSVGLNIELPFEQHFNPYIDKDKNLNFDYFFVRKVMFVKYSQGFVVMPGGFGT
                     LDEMFEAVTLIQTKKIGKFPIILVGTDYWSGLLDWIKNVMIDKFKNANPEDMNLIRVV
                     DTEDEVVEALDAFYKKYNLSPNF"
     gene            complement(5516..>5929)
                     /locus_tag="P5335_09890"
     CDS             complement(5516..>5929)
                     /locus_tag="P5335_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRZ75232.1"
                     /translation="KIFRKVKTIQEVGLGYITLGQQSTTLSGGEAQRIKLATELSKKD
                     TGNTFYILDEPTTGLHFEDIRVLMEVITKLVDKGNTVLVIEHNMDVIKLADYIIDIGL
                     EGGKGGGEVICKGTPEEIIKNKKSFTAQFLKKELL"
BASE COUNT         1904 a          962 c          953 g         2010 t
ORIGIN      
        1 ggtaaatata tttcaaaata atcggttaat aaattcaatc gaattccact ttcgtaaata
       61 aactccggtg aaatattttc attttttatc aaaccgatgt cgccatataa ttcaatccaa
      121 ttccaaatac tgtaacttgt attgagtgtt gtgagccatt tattggaaag cttagtgtct
      181 aattgagatt taaaagcacc ttccccaaca acatattgtt ggctgaaaaa cccggaggtt
      241 tcagaacggc catataaatt gtagtcaaac aaataatcat ttacttgcga aatcccaaaa
      301 tcataataat cagtatctgt attttggtat aaaaaagaac ccgcatacca tcgaatattg
      361 aattgacggt tattatcata tagttttcga aaatctactt caaaagaagc tttaccaaaa
      421 gttcggtcaa tttgtaaatc acctttaaag ctataatgac tcaaaacttc agttctaacg
      481 tttaggtatt tgacgttgaa aatagaatag tcttggtatt catccgtaga ttcagtatca
      541 ataaaatcag tttgctgtct atcaatcatc acatatcgaa agagcaattg ttctttatgg
      601 ttatcacgta aatcactttc gcgtcttctg aaaataagca ttggattaaa tttgtaaaac
      661 tctgcatcgg gagcataatg aaatcgtgaa cccgacatac tgtaaatagt ttgataccaa
      721 tcactttttc tattgttttg ggtatataaa aaactgaaag atccaacaaa tgtttttgat
      781 ttgactcccc aaaccggatt gatatcgtaa ataaaaggct tgtttagaat agttttgtta
      841 taaaaccgca taccaggcat taatccatca taaaggttgt aatttaaaat cgggacatat
      901 agaatttgat tgtattttgg gtcttctaaa tcttggaaaa agacaaattt aaaaggctta
      961 ttagtaatgt gaaaattaga tacagaacgc caattatttc gtagattaaa ttccggaact
     1021 tcattgttat aattgataac aattttgtca gcttgattat ttggcaatgt aatggtagaa
     1081 tcggtagaaa aaccttcaaa ccattctttg aaaacaactt ttttgtcttt gatgccataa
     1141 accggaaaag gaacagcagt gcctgttttg ttgtgaattg ttgccgtgat actgtcgttc
     1201 gtttttgtta cgttgtgaaa tttatagtca attaaatcac gggaatcaat tacggtttca
     1261 aaaaaccagt ttaagttttt atcggttttg attttcaatt ctttttcaaa atctgttctg
     1321 tttgtttgct gcttatggtt tttggtataa aattgtttaa taccagtaga aacaatagaa
     1381 tcacctaaat aactgtcgag gtattttaaa cttaacccgg ctctgtattt tcctgcaatt
     1441 tgttcattga attttaagga tgtatctttt aaatcaccta tcgcttggtc taaattttta
     1501 cgtgccatga gcaaataaaa gtagctgtac tgttcattaa aaccaatatc catcaagtgg
     1561 tatcctttta gtattttcaa cttactgata ttacccatca tttttaattc cgggtaattt
     1621 tcatccatgt aattcatcat gatgtagact tgaattccat cataaatcca attgtccttt
     1681 cttgggtcta agcttaatga tttttctaaa aaagtgttga tataggtttt caaaaacttc
     1741 aattcaaaca acaaatcatt tggaaacggg ctaataaaag aaggtaattg atttaagcca
     1801 taaaatggat ttcgatcata atcagtttgc gatactgtga tttttgtgtg tggataggat
     1861 cctagatttt gatccacaaa atttactatt ctatcaatta caattgcttt ttgaattaca
     1921 tctactttgc tgtcattcaa attggtgact acctcaacta tatcattttt atagcttgaa
     1981 aaagtttgtg atgtttcaat gaaaagacta aaattcgttc tgttttgacc ttctaaaaaa
     2041 taagtttgat tgctcaaatc tatttcagga attccaacat ttaaatcgga ggataatttt
     2101 aaagatttag acattttaag gctcattttt atattaaacg gagcatttgc aatatcatca
     2161 agattcaaat tactgtaagt agcaaaatta ttgttttcta ttcgggcagg ggttaaaaac
     2221 caatttttta agctataacc tccacctatt tcaaatccat agtgtgtgaa tttattgttg
     2281 ggtattttga cgtaatacaa aagatttaag ataagttttt ctcccggttt caaaggtttg
     2341 ctgagtaaaa cttccacaat atcagggtgt ttgtccggtc ggcaccaatc gacaattcgt
     2401 ttttctgaat caagaattga taagttattt gtacttcctc tgtctttaaa atttgctaaa
     2461 tgaaatcccc tgtaatgctc atctgaaaaa cgttttgcca agggagtgtt tttttctgaa
     2521 aaagagttgt tccaatcgtt caatacaatt ttgtaaagag tattctcaga ggtattgtga
     2581 tagattagtt cttgttttat aagtaatgtt ttttgttccg ggtaaagttc aacaaatagg
     2641 ttgtgttgat gttgagagaa tcccacacca aacatagaaa gaaagaaaaa ggaaatagca
     2701 attttggtaa tcttattcaa aggaacgcat atattttgag gttgaaagat agtaaatata
     2761 accaaaagat aatcttaaaa atgttaaaaa cactagtgtt taagataata ttaagtaaat
     2821 ttgacaaaac taaaatacca atgacaaaaa gagtacttct ttgggtcggt ttgcttatgt
     2881 ctacttgcgt tttttcacaa gtggtaatca atgaagtaga tgctgacacc cccggtttag
     2941 acgtaaaaga atttattgaa ctcaaatctc aaactccatt tttttcactt gacggctata
     3001 tcatagtcat gtataatggc agtacttccg gaacaggaac attaagttat tacactatta
     3061 atttagcagg gttaataacg gatggaaacg gcattatttt gctcggaaat tcgcaggtta
     3121 atccggctcc ttctttattg tttcctcaaa attctgttca aaatggccct gatgcaatag
     3181 ctatatatca agatattgtt gataattttc caattgatac tccggcaaca gcattaaatt
     3241 taattgatgc tttagtttat ggtaatgggg ttacacaagc aacagaacta caaacaattt
     3301 taggggtatc aactcagcca aatgaaaatg tcaattcctt agcagcttct cagtctattc
     3361 aacgtaaaaa tgatggtacg tatgaagtta agacaccaac tccgcggacc aataatgatg
     3421 gcagtgggat tgtttacaac ggaattactc ttgccacaag tgtttctgta ttgaacgaag
     3481 gtgaaaatat agcgattaca tttacaacag atacacctgt aacgacagat ttaaatttta
     3541 ctttaaatct taccaatgga tcatttacca atgctgattt tactgggaat ttatctattt
     3601 caattccatc cggtacttca agtggttcta caactattca agtaattgat gatgggttaa
     3661 atgatggaga tgaaacgatg tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nncatgctca taattatgga gatattgtgg atattttaaa aacagcagat caaaatccgg
     3841 ctaataacaa cgaagtttgg ttaatgtacg tagaacaacc acgttcaaaa attgactatc
     3901 agttaggccc aagtacaatt ggcgtttgga atcgagagca catttggcct cagtctagag
     3961 gtggatttgc tgatggaact tcctcttttg cagatggaat aaatatatgg ttgccaacca
     4021 atgcggatga tattttgtcc ggtcatgctg atgctcatca cattagagct gaagatggtc
     4081 aggaaaacag ttctcggagt aacagagatt acggtactga ttataacggt ccaacaggaa
     4141 atttaggaac atggaaaggc gatgttgccc gttccttatt ttatatggct gttcgttaca
     4201 acggattaac tttagttaat ggaaatccgg cggataatat ttcaggacaa attggagatt
     4261 tagcttcctt gttaacttgg aattctactg atccggctga tgattttgaa atgaatcgaa
     4321 ataattatat ctatacatgg caagtaaatc gaaatccgtt tatcgattat cctaatttag
     4381 ctgattatat ttggggtgaa aattatggac aacaatggtt tccaacactt agccaaccta
     4441 aatttgatga agcaaatgtt agggtttatc caattccgac tcgtgatgaa attacaataa
     4501 gcggagttga atcgtttgcg aaagttgaaa tttatgctat aaacggtcaa caagttttat
     4561 cgaaagaaat tgaaggattc actcaattaa aattgaattt accctcaggg atgtacctca
     4621 tgaaaattca aactgaaaat caaacaatta cgaaaaagat tatcataaaa taaaaaaacg
     4681 aactaatcaa caagattaat tcgtttttaa atagccacta attcatgaaa aaataaattc
     4741 tgaattagtg ttttttttta ttagaagttt ggactcaaat tgtatttttt gtagaaagca
     4801 tccaaagctt caactacttc atcttcagtg tcaaccactc ggattagatt catgtcttcc
     4861 ggattggcat ttttgaattt gtcaatcatt acatttttaa tccaatctaa taaaccggac
     4921 caataatcag ttccaactaa aataattgga aatttcccga tttttttggt ttgaattaaa
     4981 gtgacagctt caaacatttc atccaatgtt ccaaatccgc ccggcattac gacaaatcct
     5041 tgtgaatact tgacaaacat aacttttcta acaaaaaagt aatcaaaatt caagttttta
     5101 tctttatcga tatagggatt aaagtgctgt tcaaaaggca attctatatt taacccaacg
     5161 gaagttcctt caccacgatg agcaccttta tttcctgctt ccataatacc cggtccacca
     5221 ccggtaatta cgccataacc ggctttactg attttatagg ctatattttc agctaattcg
     5281 taatatttct cacccggttt tgttctggct gaaccaaaaa tggaaacaca tggcccaatt
     5341 ctggacatgc tttcataacc attcacaaac tcagacatga ttttaaaaat ggcccaagaa
     5401 tcattagttc ggatttcgtt ccaagttttt tgtttgaact tttcttgtat tctgtgttct
     5461 tcgctttcgt attgatctac agacattcta tcttttttta aattaaacaa acaagctaaa
     5521 gtaattcttt tttcagaaat tgagcagtga aactcttttt gtttttgatg atttcttcag
     5581 gggttccttt gcagatgact tctccgccac cttttccacc ttctaaaccg atgtcgataa
     5641 tataatctgc taacttaatt acatccatat tgtgttcaat cactaaaacg gtatttcctt
     5701 tatctacaag ttttgtaatc acttccatta atacccgaat atcttcaaaa tgtaatccgg
     5761 ttgtgggttc atctaaaata taaaatgtat taccggtatc ttttttggaa agttctgtgg
     5821 ctaatttaat acgttgagct tcaccacctg aaagtgtagt gctttgttgt cccaatgtga
     5881 tatagcctaa accaacttct tgaatagttt ttacttttcg gaatatttt
//
LOCUS       DAOTCW010000141         5799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_10277_length_5799_cov_31.036038, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000141 DAOTCW010000000
VERSION     DAOTCW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10277_length_5799_cov_31.036038"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1821)
                     /locus_tag="P5335_09895"
     CDS             complement(148..1821)
                     /locus_tag="P5335_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRZ75233.1"
                     /translation="MKNTEFTPKLITLLKEGITKKQLGKDVLAGLIVGIVALPLAIAF
                     AIASGVSPEKGLITAVIAGITISVLSGSRVQIGGPTGAFIIIIYGIVQEHGIGGLTIA
                     TFMAGFIIIAMGLARLGNYLKFIPYPLIVGFTSGIALVIFSSQIKDFFGLQMNDVPVD
                     FIDKWIAYGANFQQINWISLAIAFGTVLIALNFNRISSKIPGSIIAIFLSTALVYFFE
                     LPVETIESKFGEIPNHISFPDFPEINFAIIKKLIQPAIVIALLGSIESLLSAVVADGM
                     MGGRHRSNMELIAQGTANIFSGLFGGIPATGAIARTATNIKNGGRTPIAGIVHGVVLL
                     LIMLLFAPIAKLIPLSCLAGILMVVAWHMGEWHHFFALLKSNRMDVIVLLTTFFLTVF
                     FDLILAIEIGMILSSFIFMKRMSEATSIQNTSNMFKSDDESGEILFEQEIPNIPKGVL
                     LYEINGPLFFGASQKFQEVITDLKQQPKILILRMRNVPFIDATGINRLKEICQQLNGK
                     GTTIIISGANHEVKAELLKADLYTMLGKYNIQDNIGKAIERAKEILKDK"
     gene            complement(2584..3291)
                     /locus_tag="P5335_09900"
     CDS             complement(2584..3291)
                     /locus_tag="P5335_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HRZ75234.1"
                     /translation="MDWITELAQNKEIPLLAAFALGLLTAVSPCPLATNITATAYIAK
                     TINSKSKVILSGLLYTLGRLFSYTGIAAIIYFGASKFHIAKAFQGNGEKFIGPIMLLI
                     GLIMLDIIKLNWISGGSLTEKLTEKFKDKGLLGSFLLGALFAMAFCPYSGALYFGMLI
                     PLTLSSDAGLAVPIVFSLGTGLPVLLFAFIIAFSIEKLGKYFNAIKKVEKVMRFITGI
                     AFILTGLYYINIYLKWI"
     gene            complement(3327..3752)
                     /locus_tag="P5335_09905"
     CDS             complement(3327..3752)
                     /locus_tag="P5335_09905"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HRZ75235.1"
                     /translation="MKTISLKLATIILAIMISSCQGNSKAETAGSKENTSKEIAENQI
                     QVIQFHSENRCMTCNKIEALTLETLKSYPELQFQLINVDDTKNEKIAEQFEATGTALF
                     LYNPTTQNKLNLTEFAFMTAGDEAKFSKELKIEIDKFVK"
     gene            complement(3766..4221)
                     /locus_tag="P5335_09910"
     CDS             complement(3766..4221)
                     /locus_tag="P5335_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRZ75236.1"
                     /translation="MKTKTQLFLELWIEARTRFTNQLEAITATDVTKKLAPSPNSVGF
                     LIRHMGEVELLFAKNVFGASNVKITAKTVIDKYDTGEWTDLQELKTDVNHSFETLKAI
                     VEQQTDSDWETIITTKEFGTKTKAEAFGRIVSHTAYHAGQLAIINKYGH"
     gene            complement(4225..5199)
                     /locus_tag="P5335_09915"
     CDS             complement(4225..5199)
                     /locus_tag="P5335_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016420840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRZ75237.1"
                     /translation="MFNWLQNLADWLIYSVFGIDVTSQLGKALNFFVYDTIKILLLLF
                     LIVFIMGIVNSYFPVEKIRNFLSRNKLFGFEYVLASTFGAITPFCSCSSVPLFIGFVK
                     GGIPLGVTFAFLITSPLVNEVAIALFIGMFGLKATLIYVTSGILLGTIGGIILNSLKL
                     EKHLSPWVQGILAKAEKEGDLEEENQTLTQRLPLIFSEAKSIIKGVFWYIIIGIGIGA
                     LMHGFIPTGFFEEYISKDNPFAVPLAVIIGVPMYSNAAGILPVIQVLVAKGIPIGTAI
                     AFMMAVVGLSIPEATLLKKVMTWKLIAIFFSVVTLCIIISGYLFNLIL"
     gene            complement(5256..5610)
                     /locus_tag="P5335_09920"
                     /pseudo
     CDS             complement(5256..5610)
                     /locus_tag="P5335_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
BASE COUNT         1698 a         1048 c         1165 g         1888 t
ORIGIN      
        1 tcattttgtt ggtcggtaaa gtaatctgat ttagtaattc ccatgcttat atattatatt
       61 gcaatattac gatataatac taaataataa aataaagttg attgtttttt ttacaataat
      121 aaaatgatga ccgttaggga ttggctttta tttgtctttt aagatctctt ttgctcgttc
      181 aatggctttt ccgatgttgt cttggatatt gtatttaccc agcatggtat acaaatcagc
      241 tttcagcaat tctgctttta cctcatgatt tgctccggaa atgatgattg tggttccttt
      301 gccattcaac tgttgacata tttctttgag tcggttaatt ccggtagcat cgataaaagg
      361 gacgttacgc attcggagga ttaaaatttt aggctgttgc tttaaatcag tgatgacttc
      421 ttggaatttt tgggaggctc cgaaaaacaa tgggccattg atttcatata atagaacacc
      481 ttttggaatg ttagggattt cttgttcaaa taatatttca ccactttcgt cgtctgattt
      541 aaacatgttg gaggtgtttt gaatggaagt agcctcactc attcgcttca tgaaaataaa
      601 gcttgataat atcattccga tttcaatcgc taaaatcaaa tcgaagaaga cggtaagaaa
      661 aaaggtggtg agcaacacga taacgtccat tcggttgctt ttcagtaaag cgaaaaaatg
      721 atgccattct cccatgtgcc atgctacgac cattaatatt ccggctaaac aagaaagggg
      781 aatcagtttt gcgatagggg caaaaagtaa catgatcagc aacagcacta ccccgtgtac
      841 aattcctgcg ataggcgttc gcccaccgtt ttttatatta gttgctgttc tggcaatggc
      901 tccggtggca ggtattccac caaacaaacc ggaaaaaata tttgcggttc cttgggcaat
      961 cagttccata tttgaacgat gccgtccgcc catcattccg tctgccacaa cagcggatag
     1021 tagggattca atacttccta acaaggctat gacgatggca ggttgaatta gttttttgat
     1081 aatggcgaaa ttaatttcag gaaagtcggg gaaagaaatg tggttgggaa tttctccaaa
     1141 tttactttca atggtttcta cgggtaattc aaagaaatac actaaagcag tactcaaaaa
     1201 aatagcgata atagacccgg gtatttttga ggaaatccga ttgaaattca aagcaattaa
     1261 aacagtgcca aaagcaatcg ccaatgaaat ccaatttatt tgctgaaaat tagcaccata
     1321 ggcaatccat ttgtctataa aatcaaccgg gacatcattc atttgtaaac caaagaagtc
     1381 tttaatttga gaggaaaaga tgactaaagc aatgccgctg gtaaagccga caatcaaagg
     1441 gtagggaata aatttcaggt agtttccaag tcgggctaat cccatggcaa tgatgatgaa
     1501 cccggccatg aacgtagcaa tggtgagtcc gcctattcca tgttcttgta ctatgccata
     1561 aataatgatg ataaaagcac cggttggccc accaatttga acccgactac cacttaaaac
     1621 ggagattgta ataccggcta tcacggcagt gattaatcct ttttcggggg aaacgcctga
     1681 agcaatagca aaagcaatgg ccaacggaag agcaacgata ccgacaatca gtcctgcgag
     1741 aacatctttg cccaattgtt ttttggtaat accttctttt aaaagggtaa ttaatttggg
     1801 agtgaactcg gtatttttca tgatatgaac agataaatta ccacacagct gagtagtgga
     1861 ttacttttta caaactattc tgtaaccaaa aaccagtgtt attctgcact tttgagatca
     1921 tattcaacgt cttaaaagag cctaatctga taaagatatg ttcaaatata cgaaattgga
     1981 gtgatacatt tttcagaaag gcaggatgtt ttaataagac tcattattgg aggttatatt
     2041 attaatgcct tgaaagtcaa taaaaaagag cacatttgtg tttcatttta cttattttga
     2101 cattggtaaa ttacattata caaagtatgt ttgtttatag taatttaaaa aaggataccc
     2161 tttgaacaat aatgtaatct gctaatggga ttataaaaat atcgttaaac gcaaaggagt
     2221 attagagcgg ttatgttaag acgctaacct attccgacgt tttggtttgt ttcaccgttc
     2281 gtgaagcaac ttttctattc atattttaac ctaactatga cacctaccac ctcaagggta
     2341 tacacgatgt atatgtgtag tatctatgga tatgctattt attaaagtta ctatatagtt
     2401 actatatact tactataaag ttactatata cttagtacaa agttactata ttttttgttt
     2461 taggtagtgt ggagcgaacg gttactaggt aaaagtgata tagctactta aaggtgaaaa
     2521 aaaagggtct attgcgggtt taaagaggag gtgattttca gtgaaaaagg ggtgtttttg
     2581 tgtttatatc cactttaagt agatgttgat gtaatacaac ccggtgagta tgaacgcgat
     2641 tccggtgatg aaacgcatga ctttttctac ttttttgatg gcattgaagt atttgccgag
     2701 tttttctatg ctgaaggcga tgatgaacgc gaacagtaaa acgggcaaac ctgttcctaa
     2761 agaaaatact atcggcactg cgaggcctgc gtctgaggag agggttaacg gaatgagcat
     2821 gccaaaatat aaggcaccac tatagggaca aaaagccatt gcgaaaagtg ctcccagtag
     2881 aaatgaaccc aataaccctt tgtctttaaa cttctctgtt agtttctcgg tgaggcttcc
     2941 gccggatatc caattgagtt tgatgatgtc cagcataatc aagccaatga gtagcatgat
     3001 tgggccaatg aatttttcgc catttccctg aaaagctttg gcaatatgaa atttgctggc
     3061 tccaaaataa atgatggccg ctatcccggt ataggaaaac aatcgaccga gcgtgtacaa
     3121 gagtccgctt aaaattactt ttgacttgct gttgatggtc tttgcaatgt aggccgttgc
     3181 cgtaatgttg gtggccaacg gacacgggct caccgcggtt agcaatccta aggcaaaagc
     3241 cgccaacagc gggatttctt tattttgagc taattcagtt atccaatcca ttgtttactt
     3301 tgatcttttg gaaatttgat tgtttgttac tttacaaatt tatcgatttc tattttcagt
     3361 tctttgctga atttggcttc atcgccagcg gtcatgaagg cgaattcggt taaattcagt
     3421 ttgttttgag tggttggatt gtaaaggaat aaagcagttc ctgtagcttc aaattgttcg
     3481 gctatttttt cgttttttgt atcgtcaaca ttgattaatt gaaattgaag ttccgggtaa
     3541 ctttttaggg tttctaaggt tagggcttca attttattgc acgtcatgca gcggttttcg
     3601 gaatgaaatt ggatgacttg tatttgattt tcggctattt ccttcgaagt attttcttta
     3661 gagccggcgg tctccgcttt ggaatttcct tgacaggagc taatcatgat tgccagtatt
     3721 atcgttgcta attttaaact gattgttttc atttgtacgc tgtttttaat gtccgtattt
     3781 atttataatt gccagttgac cggcatggta tgcggtgtgt gaaacaattc ttccgaatgc
     3841 ttctgctttt gttttggtgc caaattcttt ggtggtgatg atggtttccc aatcgctgtc
     3901 ggtttgttgt tccactattg ctttcaacgt ttcaaacgaa tggtttacgt ctgtttttaa
     3961 ttcttgtagg tctgtccatt ctccggtgtc gtatttatcg atgaccgttt tggccgttat
     4021 ttttacgttg gaagctccaa agacattttt ggcaaacaat aattccactt cccccatgtg
     4081 gcgaatgaga aaacccacgc tattgggaga gggagctaat tttttagtta cgtcggttgc
     4141 tgtgattgct tccaactggt tggtgaatcg ggtgcgggct tctatccaaa gctccagaaa
     4201 aagttgtgtt ttcgttttca ttttttataa aatcagatta aacaaatatc ctgaaatgat
     4261 gatgcaaagg gttactacgg aaaagaaaat ggcgatgagt ttccaggtca ttactttttt
     4321 gagcagggtt gcttcaggaa tggacagccc tactacagcc atcatgaacg caatggctgt
     4381 tccgatggga attcctttgg ccaccaagac ctgaatgacg ggtaaaattc cagccgcatt
     4441 gctgtacatg ggtaccccta tgattacagc caacggcaca gcgaacggat tgtctttgct
     4501 gatgtattct tcgaagaagc cggtagggat aaaaccgtgc attagtgctc cgatacctat
     4561 tccgatgatg atgtaccaaa acacaccttt tataatactc tttgcctcag aaaagattaa
     4621 gggtagccgt tgcgtaagtg tttggttttc ttcttctaaa tcgccttctt tttcagcttt
     4681 ggctaagatg ccctgcaccc aaggagacaa gtgtttttcc agttttaaac tatttaaaat
     4741 aatacctcca atggttccca acagtatgcc gctggttaca taaatcagcg ttgcttttaa
     4801 gccaaacatt ccgatgaata gggcaatggc cacttcattg actaaaggcg aagtgattaa
     4861 aaaagcaaaa gtgactccca acgggatgcc tccttttaca aaaccgatga acaaaggcac
     4921 ggaactacac gaacagaaag gcgtgatggc tccgaaagtt gacgccaaga catattcaaa
     4981 tccaaacagt ttgttacgcg acaggaagtt tctgattttc tcgaccggga aataggaatt
     5041 gacgattccc atgatgaaaa caatcagaaa aagaagcaac agaattttga tggtatcgta
     5101 tacaaaaaaa ttcagtgctt tccctaactg cgaagttaca tcaattccaa aaacggaata
     5161 aatgagccaa tctgcgaggt tttgtaacca gttaaacatt ttgatgttaa ttacgaatta
     5221 cgaattatga attacgaatt atcaattatc aattattatt tttgagcttt catggttttt
     5281 tgaatactcc ctataattct cagtatttct tccatatcat tcatcaatga ttcgaacatt
     5341 tggttgtcta tatactctgt cgcatggagt aatcgcaacc agtattttgt ttctcttgct
     5401 tctttataag caatcgtcaa ttttgtacta aaatcatttt tgctttgtcc accaatggct
     5461 tcttctacgt ttgctcctat cgaagttcca cttcgtaaca gttgtttaga aactacaaat
     5521 tctttctttt ccgtgcttaa aaaacttata aagatttatt atccttacag caaattcaaa
     5581 agtttttgtt tgtatgatgt tgtctttcat ttgttctgat tagttgaatt tatactctaa
     5641 aaattcgtaa ttcctaattc gtaattccta attatttaac agcatcctga cgtagaacaa
     5701 cacgagtctg tgccggatac gatggaggtg tcgcctttgg ttgagaagcc tttttggacc
     5761 caacgaatga tgccgtgttt catgtttacg acattgtca
//
LOCUS       DAOTCW010000142         5772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_10335_length_5772_cov_40.760189, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000142 DAOTCW010000000
VERSION     DAOTCW010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10335_length_5772_cov_40.760189"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..>2079
                     /locus_tag="P5335_09925"
     CDS             128..>2079
                     /locus_tag="P5335_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRZ75238.1"
                     /translation="MLDKIIHFSINNKFIIGLFTLVLVIVGSYSLYTLPIDALPDITN
                     NQVQIITSSPTLATQEVELFITYPIEQSVKSIPRVVELRSISRFGLSVVTVVFEEDVD
                     IYWARAQITERIKDAENTIPKGVGTPEMAPVSTGLGEIYQYVVFPKKGYEEKYNATDL
                     RTIQDWIIKPQLIGTKGVAEVNTLGGNLKQYEIAVVPDKLRSMNTTITEIFEALENNN
                     ENTGGAYIDKKPYAYFIRGVGMVSSIDDINKIVVKNQNGIPILIRDVANVQIGSSIRY
                     GAVTKDGKGEEVSGMVMMLKGENSGEVVKVVKDKMEQIKKSLPEGVEIEAFMDRTVLV
                     DKAISTVQTNLIEGALIVVFILVLLLGNWRAGLVVASVIPLALLFAISMMKLFGVSGN
                     LMSLGAIDFGLIVDGAVIIVEAIIHRLQVSKKGKLTAKEMNDEVYHASSKIRSSAAFG
                     EIIILIVYLPILALVGIEGKMFGPMAQTVSFAILGAFILSLTYVPMMSALALKKQTGH
                     KKNFSDKIIDTIYNFYNPILEKALQAKAAIIAVAVALFVASLFLFNSLGGEFIPTLDE
                     GDIATHLIIASGSSLSQEVEATTKAEQILRDKFPEVKMIVTKIGSAEIPTDPMPIEAG
                     DMIILLKDKSEWTSAETKEELMEKME"
     assembly_gap    2080..2179
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2180..4174
                     /locus_tag="P5335_09930"
     CDS             <2180..4174
                     /locus_tag="P5335_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRZ75239.1"
                     /translation="ALFVTLPSGNQIPLEQIADVKYEDGPMQISRENGKRRIVVGFNV
                     RGADVKTVVETIQAKLDDKLKLPEGYYTTYGGQFENLIQANKRLSVAVPVALGLILVL
                     LYFTFKSIKQSLLIFTAIPLSAIGGIVALWLRDMPFSISAGVGFIALFGVAVLNGIVL
                     IGYFNELKKQGMDNVFDRIKEGTKVRLRPVILTAAVASLGFLPMAISTSSGAEVQKPL
                     ATVVIGGLITATLLTLIVLPILYLYFEKGIKIKRKTMKKTTMLLAFLVSSMAINAQEK
                     KKLSLNEALEMGLKNNLNVQAVTLETKMQSQLVKTAFELPKTEISGTYGQINSQAQDK
                     NFQISQSFNPFQISAKRKLLQENNSSSQNKLSATKQEITFSIRQSWNTILYYEKQMAL
                     LEKQNELMQKFVKSATLKFQTGETSALEKTIAVAKQQELEQKIKQYKTQIAIEKSKLK
                     TLLDIDNEIVFSDTTFVPMPFVVKIDSTLVKQNPVVQLANQQVKIAEANHKLEKSNLF
                     PEFSVGYFLQSLTGNQEVDGVTKYYDDSLQFQGFSVGLSVPIFMGSTIKKSKAAQTNI
                     EMEQINANYINQQFKSHLEQQMEQLTTYQSLIDYYKNTAIPNADLIIKNATKSYQNGD
                     ISYVEYVQGIETATQIQTNYNEAINNYNQTIIAIQYTINQ"
     gene            4186..5472
                     /locus_tag="P5335_09935"
     CDS             4186..5472
                     /locus_tag="P5335_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRZ75240.1"
                     /translation="MKKSNIIYASIVGVIILAVILYFSLRHTEGDGHNHAEGETHSEE
                     SHSDEKEPTAIKEVELNEAQFKASSIELGTFSDKNLSEVINVNGYTKLPPQNQADVSV
                     YTSGIVKTISVSEGQYVSKGQIIATIESPEFTKLQEAYLTSKSNLEFLKLEFERQKTL
                     SEEDVNSKKTFQKTKAEYEIERARFNSLQKQLNTLNISDNGIATATVPVLAPISGNLT
                     EIYIKIGSNVEAGKPLMNIVDNSKLHVDLLVYEKDLFKVKIGQNVRFVLVNQDNSEVK
                     GKIFSVGKSFENDTKSVAVHADITNNQQKLIPGMYVSALIDVGSNNVKAVPTDAIIKA
                     DGREFIFVLEEGHKEEAHDEKEGHNHEDGDKHEEAEGKTFHFQRIEVKSGTTQLGYTQ
                     VTLLQKIDANAKIVLKGSYYIQSHLIKNEGGGGHAH"
     gene            5513..>5772
                     /locus_tag="P5335_09940"
     CDS             5513..>5772
                     /locus_tag="P5335_09940"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRZ75241.1"
                     /translation="MKKSILILALAIALGTTVSCNKKEETTKTATEHEGHNHKEGEEH
                     KVAYECPMDCEKGKTYDEKGTCPVCKMELIEAKAENHEGHDH"
BASE COUNT         2163 a          828 c         1030 g         1651 t
ORIGIN      
        1 agtatttggc aaccacctca aatagtataa tgaagcccga gtaatttcgg gtcaataagt
       61 tgtgtcttta aaacaactta caaataaata acagttctct gttttataat tcattataca
      121 aaataaaatg ttagataaaa tcattcattt tagtatcaat aataaattta ttattggtct
      181 ttttacactc gtattagtca tagtaggtag ttattcctta tacactttac ctatagatgc
      241 gttaccggac attacaaata atcaagtaca aatcattaca agttctccta cacttgcaac
      301 gcaagaggtt gagctattca taacttatcc gattgaacaa tcggttaaat caattcctag
      361 ggttgtagaa cttcgttcca taagtcgttt tggcttaagt gtggtaacgg ttgtttttga
      421 agaagatgtc gatatttatt gggcaagagc acaaataaca gagcgaataa aagatgcaga
      481 aaatacaata ccaaaaggtg ttggaactcc tgaaatggct ccagtaagca caggtttagg
      541 cgaaatatac caatatgtag tatttcccaa aaaaggatac gaagaaaaat acaacgctac
      601 tgatttaaga accattcaag attggatcat aaaaccccaa ttaatcggta caaaaggtgt
      661 agccgaagta aatactttag gtggaaacct aaaacaatat gaaatagctg tggttccaga
      721 caagctaaga agtatgaata caaccatcac tgaaatattt gaagcattag aaaataataa
      781 tgaaaataca ggtggtgcat acatagataa aaaaccgtat gcttatttta ttagaggggt
      841 cggaatggtt agtagcatcg acgatataaa taaaatagtg gttaaaaatc aaaatggaat
      901 acctattctt attcgcgatg tagccaatgt gcaaataggg agcagtattc gttatggtgc
      961 agtaaccaaa gacggtaaag gtgaagaagt ttcgggaatg gtgatgatgc ttaaaggtga
     1021 gaacagtggt gaagtagtga aggtggtgaa agacaaaatg gaacaaatca aaaaatcact
     1081 gccggaaggg gtagaaattg aggcgtttat ggatagaacc gttttagttg acaaagctat
     1141 aagtacagtg caaactaatc ttattgaagg agcattaatt gttgttttca ttttagtttt
     1201 actcctgggt aattggagag ctggtttagt ggttgcttcg gttattccat tggcattatt
     1261 gtttgccatt tctatgatga aactttttgg agtgagtggt aatttaatga gtttaggtgc
     1321 tatcgatttt gggctaattg tagatggtgc tgttattata gtagaagcta ttattcatcg
     1381 actccaagtc agtaagaaag gtaaactaac tgccaaggaa atgaatgatg aagtgtacca
     1441 tgcttcttca aaaattagaa gtagtgcggc ttttggagaa ataatcatct taattgtata
     1501 tctgcctatt ttagcgttag tagggatcga aggtaaaatg tttggaccaa tggcacaaac
     1561 agtttcattt gccatattag gagcatttat attgtcttta acttacgtcc ctatgatgtc
     1621 ggctttggca ttaaaaaaac aaacaggaca taaaaagaat tttagtgata aaattatcga
     1681 taccatttat aacttttaca atccaatttt agaaaaagca ctccaagcta aagcagcaat
     1741 tattgcggtt gctgtggcat tatttgtggc ttctttattc ctctttaatt cattgggtgg
     1801 cgaattcatt cctactcttg acgaaggtga catcgcaact catttaatca ttgcttcggg
     1861 tagttcatta tcgcaagaag tggaagcaac tacaaaagca gaacaaattt taagagataa
     1921 gtttcccgaa gtaaaaatga ttgtaaccaa aattggttcg gctgaaattc ctacagaccc
     1981 aatgcccatt gaagcaggag atatgattat tcttttaaag gataaaagtg aatggacatc
     2041 agctgaaacc aaagaagaac taatggaaaa aatggaacan nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnng cgttatttgt aacattacca tcaggcaatc aaattccgtt
     2221 agaacaaatt gctgatgtaa agtatgaaga tggaccaatg caaatttctc gtgaaaatgg
     2281 aaaacgtaga atcgtagtag gttttaatgt tcgtggtgct gatgtaaaaa cagtggtaga
     2341 aaccattcaa gctaaattag acgataaact aaaattacct gaaggatatt acactactta
     2401 tggcggacaa tttgaaaatc ttatccaagc aaacaaacgt ctttctgttg ctgtgccggt
     2461 agcattaggt ttaattttag tcctactcta ttttacattc aaatcaatca aacaatcgtt
     2521 gttaatattt actgcaattc cattatctgc tattggtggt attgtggcgt tatggttacg
     2581 cgatatgccg tttagtattt ccgcaggtgt cggatttatt gctctttttg gtgtagcggt
     2641 gttaaacggt attgttttaa tcggatattt taatgaactt aaaaaacaag ggatggataa
     2701 tgtatttgac cgaataaaag aaggaacgaa agtacgttta agaccggtaa ttttaaccgc
     2761 tgctgtagca tctcttgggt ttttaccaat ggcaataagc acaagttcag gtgccgaagt
     2821 tcaaaaacct ttagcaactg tagtaattgg tggtttaata acagctactt tactaacatt
     2881 aattgtctta cctattttgt atttatactt tgaaaaggga attaaaataa aacgtaaaac
     2941 catgaagaaa acaacaatgc ttctggcttt tttagttagt tctatggcaa taaatgctca
     3001 agaaaagaaa aaattaagtt taaatgaagc tcttgaaatg gggttaaaaa acaacttaaa
     3061 tgttcaagcg gttaccttag aaactaaaat gcaatcacaa ttagttaaga ctgcattcga
     3121 attgcctaaa acagaaattt caggaactta tggacaaata aattcgcaag ctcaagataa
     3181 aaattttcaa attagtcaaa gtttcaatcc ttttcaaata agtgcaaaaa gaaaattatt
     3241 gcaagaaaac aactcttcaa gtcaaaataa attaagtgct acaaaacaag aaattacatt
     3301 ttctattcgt caatcttgga atacaatttt atactacgaa aagcaaatgg cattgttgga
     3361 aaagcaaaat gaactaatgc aaaaatttgt caaatccgca actctaaagt ttcaaacagg
     3421 tgaaacaagt gcactggaaa aaacaattgc agtagctaaa caacaagaat tagaacaaaa
     3481 aataaaacaa tacaaaacgc aaattgctat cgaaaaatcc aagcttaaaa cacttttaga
     3541 tatcgataat gagattgtat tttcggatac tacttttgta cctatgcctt ttgttgtaaa
     3601 aatcgatagc acgttagtga aacaaaatcc ggttgttcag ttagctaatc aacaagttaa
     3661 aatagctgaa gcaaatcata aattagaaaa atcaaattta tttccagagt tttcagtagg
     3721 ctactttttg caatccttaa cagggaatca agaagtggat ggtgtaacga aatattatga
     3781 tgattcacta caatttcagg ggttttctgt tggtctttca gtgccgatat ttatgggtag
     3841 cactattaaa aaaagtaaag ctgctcaaac aaatatagaa atggagcaaa taaatgctaa
     3901 ctatataaac cagcaattta aaagtcattt agaacaacaa atggagcaac ttacaaccta
     3961 tcaatccttg attgattatt ataaaaacac agcgattccg aatgctgatt taataattaa
     4021 aaatgctaca aaaagttatc aaaatggaga tatttcgtat gtagaatatg tacaaggtat
     4081 tgaaacggct acccaaatac aaaccaatta taacgaagct ataaataatt acaatcaaac
     4141 tataatagct atacaatata ctatcaatca ataacataaa cagaaatgaa gaaatcaaat
     4201 atcatatacg catcaatagt tggagtaata atccttgctg ttattttata tttctcttta
     4261 cgtcatacgg aaggggatgg acataatcat gcagaaggcg aaacacattc agaagaaagt
     4321 cattcagatg aaaaagagcc tacagcaata aaagaagtag aacttaacga ggctcagttt
     4381 aaagcctcaa gtatcgaatt aggaactttt tcagataaaa atttaagcga agtaataaat
     4441 gtaaatggtt ataccaagtt acctcctcaa aatcaagccg atgtttcagt ttatacatca
     4501 ggtattgtaa aaacaatttc tgtatcagaa ggacagtacg taagtaaagg tcaaatcatc
     4561 gcaactattg aaagtccgga gttcactaaa ttacaagaag cttatttaac ttcaaaaagc
     4621 aatttggagt ttttaaaatt agaatttgaa agacaaaaaa cattaagcga ggaagacgta
     4681 aattctaaaa aaacgtttca aaaaacaaag gcagaatacg aaattgaaag agctcgattt
     4741 aattcgcttc aaaagcaatt aaatacttta aatatcagtg ataatggaat cgcaacagca
     4801 accgttcctg tgcttgctcc aatttctgga aacttaaccg aaatttatat taaaatcgga
     4861 agtaatgtag aagcaggaaa accgctaatg aatatagtag ataattcaaa attacatgta
     4921 gatttattag tctatgaaaa agatttattt aaggtaaaaa taggtcaaaa tgtacgtttt
     4981 gttttagtga atcaagataa ttcagaggta aaaggaaaaa tatttagtgt cggtaaatct
     5041 tttgaaaacg atacaaaatc agtagctgta catgcagata ttacaaataa ccaacaaaaa
     5101 ttaattccgg gaatgtatgt aagtgctctt atagatgtag gttcaaataa tgtaaaagca
     5161 gtaccaacgg atgcaattat taaagctgat ggtcgcgaat ttatttttgt tttagaagaa
     5221 ggacataaag aagaagctca cgacgaaaaa gaaggacata atcacgaaga tggcgacaag
     5281 catgaagaag cagaaggaaa aacatttcat tttcaacgta ttgaggtaaa atcaggcacc
     5341 acacaattag gatatacaca agttacgcta ttacaaaaaa tcgatgcaaa cgctaagatt
     5401 gttttaaaag gatcgtatta cattcaaagc cacctaatta aaaacgaagg tggtgggggt
     5461 catgcacatt aaaaattaat aatataaatt ataatctaaa ttcaattatc aaatgaaaaa
     5521 atcaatttta attttggcac ttgccattgc tttaggtact accgtttctt gtaataaaaa
     5581 agaagaaact acaaaaactg caacagaaca cgaaggacac aatcataaag aaggtgaaga
     5641 gcataaagtt gcttatgaat gcccgatgga ttgtgaaaaa ggtaaaactt atgatgaaaa
     5701 aggaacttgt cctgtttgta aaatggaatt aattgaagcg aaagcggaaa accacgaagg
     5761 tcatgatcat ga
//
LOCUS       DAOTCW010000143         5645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_10696_length_5645_cov_23.248301, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000143 DAOTCW010000000
VERSION     DAOTCW010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10696_length_5645_cov_23.248301"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..1667
                     /locus_tag="P5335_09945"
     CDS             318..1667
                     /locus_tag="P5335_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HRZ75242.1"
                     /translation="MSKDIRIKKGLDLKLKGESVKKIADVARSKVYAIKPSDFHGVTP
                     KMVLKEGDSVKAGEVIFYSKTDEKIKFVAPVSGKIQEIKRGEKRVILEILIAADTQDV
                     FIEHSKKNPNDLSAEEVRAHLLASGCWPFINQRPYDVIANSVDTPKAIFISAFSTAPL
                     AADVEYVLENKLQAFQTGVDALAKLSNGKVHLSISGKGKSIFQAVKGVTLHQVYGPHP
                     AGNVGVQISKISPINAGEKVWTVKPQDVAVIGELFLSGKFNATRTIALAGSEVKEPQY
                     YNVTVGAQVEDLVSEKLKGVSVRIISGDVLSGNKVTLNGNIGFYTNMVTVIPEGNNYR
                     MFGWMPFTSPNIHSASRTGLSWLMPGKKYEPNTNLNGEERALVVTGEMEEVMPLDIYP
                     MQLLKACLAGNIDKMESLGIYEVAPEDFALIDYTNTSKLEAQEIIREALDLMIKEVG"
     gene            1675..2838
                     /locus_tag="P5335_09950"
     CDS             1675..2838
                     /locus_tag="P5335_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HRZ75243.1"
                     /translation="MKFLRDKIDQIRKPFEKGQKFEKFAPAINALDTFLYVPNHTTKH
                     GAHIRDAVDLKRTMITVVLALVPALLFGIYNSGYQHFVQIEGADMSFWNLFSHGLWKV
                     LPMIIVSYVVGLGIEFGFAIWKGHEVNEGYLVTGLLIPMVMPIDFPLWMLAISVAFAV
                     VIGKEVFGGTGMNIFNPALLARAFAFFAYPTFMSGDKIWVSEASTTDAISGETILGTL
                     AQGKEVTYSTMDMFLGFIPGSVAETSTLAVLIGALLLIVTGVGSWRIMASGVLGAIVT
                     TLMFNAFGLTSLMSFAWYEHLIVGGFAFGIVFMATDPVSAAQTAKGKIYYGFLIGFFA
                     MTIRIFNPAYPEGIMLSILLMNSFAPTIDYYVVNSNISKRMKRLAKSKKIKTA"
     gene            2841..3524
                     /locus_tag="P5335_09955"
     CDS             2841..3524
                     /locus_tag="P5335_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRZ75244.1"
                     /translation="MNREANSYTFLFATVMVVIVATALAFAATTLKPAQDENIQNEKR
                     QYILKSFGINVERDSSVSAYKKYIKAEYVFDVNGNEIGNDAFNIDIAVTKDKFPVFVT
                     EKEGNKFYVIPVRGVGLWDAIWGYISINEELKVDGIVFDHKAETPGLGAEIREDFFTK
                     RFVGESIFDANGSLQAVKVVKGYTGGDNKEDGEVDAISGATLTGNGVTNMLASGLKPY
                     EKFLKSHKK"
     gene            3530..4192
                     /locus_tag="P5335_09960"
     CDS             3530..4192
                     /locus_tag="P5335_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HRZ75245.1"
                     /translation="MSDKKEGLFSKSNIKLVTNPFNDDNPVTVQVLGICSALAVTVQM
                     KNAFVMALSVTVVVAFGNVIISLLRNLIPNRIRIIVQLVVAAALVICVDQVLKAYAYD
                     VSKQLSVFVGLIITNCILMGRFEAFALGNKPWPSFLDGLGNGLGYGIILMIVAFFREL
                     LGSGKLFGIELIGSSVEKTGAYASGYVNNGFMLLAPMALIIVGLIIWVQRGINKKLIE
                     KN"
     gene            4198..4965
                     /gene="nqrE"
                     /locus_tag="P5335_09965"
     CDS             4198..4965
                     /gene="nqrE"
                     /locus_tag="P5335_09965"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HRZ75246.1"
                     /translation="MFEYFNIFLKSMFIENMVFAYFLGMCSYLAISKKVSSALGLGIA
                     VTFVQVMTVPLNYLMYEYILKPGALQWLDPTMAELDLSFLTFILFIASVATIVQLVEM
                     IVEKFSPSLYNTLGIFLPLIAVNCAILGGSLFMQQRVDSGTYANFGHVLTFAFSSGIG
                     WAIAIVLLAAIREKMEYSNVLPPLRGLGITFITTGLMAIGFMSFGGVDLNAFNKKPQP
                     KVDEVPVTTVTPEIVPVVDSLQVVVDSVKVDSNQVVK"
     gene            4991..>5645
                     /locus_tag="P5335_09970"
     CDS             4991..>5645
                     /locus_tag="P5335_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRZ75247.1"
                     /translation="MFSIAELIIYSILAFLVLILLMVYMLLYAKSKLVSSGAVKIKIN
                     GENEIEVNAGSTLLSTLGNSKIFLPSACGGGGTCLQCKCKVLKGGGEALPTEVPNFTR
                     KELADGWRLGCQVKVKNDMIIEVPEEVFGIKKFEAKVYSNYNVASFIKEFIVELPDDM
                     HYEAGGYIQIEIPECEVKYSDIDITAHPEEHPGEPEKFKAEWDKFNLWPLVMKNPELV
                     "
BASE COUNT         1860 a          698 c         1122 g         1965 t
ORIGIN      
        1 acaaaaaagt aaatataata ttatttgttt acataaaaga tgccaaattt aatttgattc
       61 tatgtttttt atttgtaaat caattcaggt tgtctttgaa gcattatttt attttttttg
      121 gttgaatatc atcggttatg ttttttttaa tttaacttta aaatataaag tgaaccaagg
      181 ttttatttag aattgatata aataaataaa aatcgattag acatttgtta atccttaatg
      241 tctattgttt taaaattttt aaatttgcac cgttttcaat taagtaaatt catttaataa
      301 tcatatcttc ataacatatg tctaaagaca ttcgaattaa aaaaggttta gaccttaagt
      361 tgaagggtga gtcagtaaaa aagattgctg atgtagctcg atcgaaagtc tatgctatta
      421 aaccttctga ctttcatgga gtaactccaa aaatggtatt aaaagaaggg gacagcgtca
      481 aagcaggaga agtaattttc tactctaaaa cagatgagaa aatcaagttt gttgctcctg
      541 ttagcggaaa aattcaagag atcaaacgcg gtgaaaaaag agtcatttta gagattctaa
      601 ttgctgccga tactcaagat gtttttattg agcatagcaa gaaaaatcca aatgatttgt
      661 cagctgaaga agtgagagca catttattag cttcaggatg ttggccattc attaatcaac
      721 gtccttacga tgtaatagct aattctgttg atactcctaa agctattttt atttctgcct
      781 tttcaactgc tccattagct gcagatgttg agtatgtttt agaaaataaa ttacaagctt
      841 ttcaaacggg tgttgatgct ttagctaaat tatcgaatgg aaaagttcat ttgtcaatta
      901 gtggtaaagg gaaatctata tttcaagcgg ttaaaggagt tacattgcat caagtttatg
      961 gtccgcatcc agctggtaat gttggagttc aaatttcaaa aatttcacca attaacgctg
     1021 gagaaaaggt ttggacagta aagccacaag acgttgctgt aattggtgag ttgtttttaa
     1081 gcggaaagtt taatgcaact aggacgatag ctttagcagg atctgaagta aaagagcctc
     1141 aatattataa tgttactgta ggtgcacaag ttgaagattt agtttctgaa aaattaaaag
     1201 gggttagtgt aagaattatt tctggggatg ttttatctgg aaataaagtg actttgaacg
     1261 gaaatattgg tttctacacc aatatggtaa ccgttattcc tgaaggtaat aattatagaa
     1321 tgtttggatg gatgcctttt acttccccaa acatacatag tgcatcaaga actggtttat
     1381 catggttaat gcccggaaaa aaatatgagc ctaacactaa tttaaatggt gaagagagag
     1441 ctttggttgt aacgggggaa atggaagagg tgatgccttt agatatttat ccaatgcaat
     1501 tattaaaagc ttgtttggct ggaaatatcg ataagatgga gagcttagga atatatgaag
     1561 tagcaccaga agattttgct ttaattgatt atactaacac gtcaaaatta gaagctcagg
     1621 aaataattcg tgaagctctt gatttaatga ttaaagaagt aggataatat agttatgaag
     1681 tttttaagag ataaaataga ccaaattaga aagccttttg agaaagggca aaaatttgaa
     1741 aaatttgcac ctgcaattaa tgcattagat actttcttat atgtacctaa tcatactaca
     1801 aaacatgggg cccatatcag agatgctgtg gatttaaaac gtaccatgat tacggtggtg
     1861 ttggctttgg ttccggcatt attatttgga atttacaatt caggttatca acattttgtt
     1921 caaattgaag gtgctgatat gtcattttgg aatttatttt cgcatggttt gtggaaagta
     1981 ttacctatga ttattgtatc ttatgtagta ggtttaggaa ttgaatttgg ctttgctata
     2041 tggaaaggtc atgaggtaaa tgaagggtat ttagtgacgg gattgctaat accaatggtt
     2101 atgccaattg attttccgtt atggatgtta gctatttcgg ttgcttttgc agtggtaata
     2161 ggtaaagaag tatttggagg taccggaatg aatattttta atccggcatt attggctcgt
     2221 gcttttgcat tctttgcata tcctactttt atgtctggtg ataaaatttg ggtaagtgaa
     2281 gctagtacca cagatgcaat ttcaggtgaa actattttag gaacattggc acaaggtaaa
     2341 gaagttactt attcaacaat ggatatgttt ttagggttta taccaggatc cgttgcagaa
     2401 acatctacct tggcggtatt aattggtgct ttgttgctaa ttgtaacagg tgttggaagt
     2461 tggagaatta tggcttcagg tgttttagga gcaatagtta ctactttaat gttcaacgca
     2521 ttcggtttaa cttctttaat gagttttgct tggtatgaac atttaattgt tggtggattt
     2581 gcttttggaa tagttttcat ggcaaccgat cccgtttcag ctgctcaaac tgcgaaagga
     2641 aaaatatatt atggtttttt aattggtttt tttgctatga ccattagaat ttttaacccg
     2701 gcatatcctg aaggaattat gctgtccata ttattaatga attcgtttgc accaacaatt
     2761 gattattatg ttgtaaatag taatataagc aaaagaatga agcgattggc aaagtcaaaa
     2821 aaaattaaaa ctgcataatc atgaatagag aagctaatag ttataccttt ttatttgcta
     2881 cggttatggt tgtaatagtt gctacagcac ttgcttttgc tgctacaacc ttaaaacctg
     2941 ctcaagatga aaacattcaa aatgaaaaaa gacaatatat cttaaaatcg tttggaatca
     3001 atgttgagag ggattcttct gtttcggctt ataaaaaata cattaaagcg gaatatgttt
     3061 ttgatgtgaa tggaaatgaa ataggcaatg atgcatttaa tattgatatt gcagtaacta
     3121 aagataaatt tccagtattt gtaactgaaa aagaaggaaa taaattttat gttatacctg
     3181 ttagaggtgt aggtctttgg gatgcaattt gggggtatat ttcaataaat gaagagctta
     3241 aagtagatgg aattgtattt gatcacaaag cagaaacgcc tggtttaggt gctgaaataa
     3301 gagaagattt tttcaccaaa agatttgtag gtgaaagtat ttttgatgca aacggtagtt
     3361 tgcaagctgt taaagttgtt aagggttata caggcggaga taacaaagaa gacggtgaag
     3421 tcgatgctat ttcaggagcc acattaacag gtaatggagt tacaaatatg ttggcatctg
     3481 gtctgaagcc ctatgaaaaa tttttaaaat cacataaaaa ataatcaaga tgtcagataa
     3541 aaaagaagga ttattttcaa aaagtaatat taagttagtt acaaatccat ttaatgatga
     3601 taatcctgtc accgttcaag tgttgggtat atgttcagca ttggctgtga cagttcaaat
     3661 gaaaaatgct tttgtaatgg cactatctgt tacagttgtt gttgcatttg gtaacgtgat
     3721 tatatcttta ttacgaaatt tgattccaaa ccgaattaga atcatagttc aacttgttgt
     3781 agcagctgcg ttagtaattt gtgttgacca agtgttaaaa gcctatgcat atgacgttag
     3841 taaacaactt tcggtttttg ttggtttaat tataaccaat tgtatcttga tgggtcgttt
     3901 tgaagcattt gctttaggaa ataaaccttg gccttcattc ttagacggat tgggaaatgg
     3961 attaggctat ggtatcattt taatgattgt tgcttttttt cgagaattat tagggtctgg
     4021 aaaattattt ggtattgaat taataggaag ttctgttgaa aaaacaggag catatgcttc
     4081 gggatatgta aacaatggat ttatgctttt ggctccaatg gcattaataa ttgttggatt
     4141 gattatttgg gttcagaggg ggataaataa aaaattaatt gagaaaaatt aataattatg
     4201 tttgaatatt ttaatatatt tttgaaatcc atgttcatcg agaatatggt ttttgcatac
     4261 ttcttaggaa tgtgttctta tttggcaatt tctaagaaag tttcctcagc tcttggtctt
     4321 ggtattgctg taacttttgt gcaagttatg accgttccct taaactattt aatgtacgag
     4381 tatatattaa aaccgggtgc tcttcaatgg ttagatccta caatggctga attggattta
     4441 agttttttaa cttttatctt atttattgca tctgtagcaa ctattgttca attagttgaa
     4501 atgattgttg agaaattttc accttcatta tataatactt taggaatatt tcttccttta
     4561 attgcagtta actgcgcaat tttaggagga tctttattta tgcaacaacg tgtagattca
     4621 ggaacttatg caaattttgg acatgtatta acatttgcat tttcctcagg tataggttgg
     4681 gcaatagcaa ttgttttatt agctgccatt cgtgaaaaaa tggaatattc aaatgttctt
     4741 cctccactta gaggacttgg aataacattt ataactactg gtttaatggc tattggtttt
     4801 atgagttttg gtggtgtaga tcttaatgca tttaacaaaa agcctcaacc aaaagttgat
     4861 gaggttccag taacaactgt aactccagaa atagttccgg ttgtagactc tttacaagta
     4921 gttgttgatt ctgtaaaagt tgattcaaat caggtagtta agtaataaca taacatttaa
     4981 aattaaactc atgtttagta tagcagaatt aattatatat tcaattttag cttttttagt
     5041 gcttatttta ctaatggttt atatgctatt gtatgcaaaa tcaaagttag tatcttcagg
     5101 tgcagttaaa attaagatta atggagaaaa tgaaattgaa gtaaatgcag gaagcacttt
     5161 gttatctaca ttaggaaaca gtaagatatt tttaccatct gcttgtggtg gtggtggtac
     5221 ttgtttacaa tgtaaatgta aagtactaaa aggagggggt gaagctttac caactgaagt
     5281 acctaatttt actagaaaag aattagcaga tggatggaga ttaggatgtc aagtaaaggt
     5341 aaaaaatgac atgattattg aagtccctga agaggtattc ggtatcaaaa aattcgaagc
     5401 aaaagtatat tcaaactaca atgtagcttc tttcattaaa gaatttattg tagaattgcc
     5461 agatgatatg cattatgaag ccggaggtta tattcaaata gaaattcctg aatgtgaagt
     5521 aaaatattct gatattgaca ttacggcaca tcctgaagaa catccgggag aacctgaaaa
     5581 attcaaagcg gaatgggata aatttaattt gtggccatta gtgatgaaaa atcctgaatt
     5641 agtag
//
LOCUS       DAOTCW010000144         5573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_10885_length_5573_cov_26.839072, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000144 DAOTCW010000000
VERSION     DAOTCW010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10885_length_5573_cov_26.839072"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..630
                     /locus_tag="P5335_09975"
     CDS             328..630
                     /locus_tag="P5335_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRZ75248.1"
                     /translation="MKTIGEIIRGHREEKGMLLRQLAALLEIDSAILSKIERGERKAT
                     KEQIIKIADILGIKQNDMLIQYLSEKIAYEIADEETAKQVLKVAEKKVDYIKNNRK"
     gene            627..3959
                     /locus_tag="P5335_09980"
     CDS             627..3959
                     /locus_tag="P5335_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRZ75249.1"
                     /translation="MTTENIKTLITLLGFIPEDKKGNIWAKKYSNYDNYTITLDFEKS
                     SINYGDKIQKGDDTTSNFSSDENFVVLECIDRLLAKGYKPQDLTLEKKWKLGRSAKSG
                     KADINVTDGKTGKTVLIIECKTWGTEFEKEKERMRENGGQLFSYFQQDKATRFLCLYT
                     SQITANLLKQEKFIDYKTAIVKVEDTVEDIRKQDNQIEHTLIYQKATDVAELVKVWNH
                     KNKKQFEERGIFEEEVEPYNPGFVPLRVADLKEFNQDDAKAEYYKFEEILRHNNISDR
                     SNAFNRFISLVLAKIVDEEKTANTITEFQIKKGVDTSEELIERLQTLYAKAMRDYLGE
                     TVINYTFEEIEQIISHFPRQTSKEDLKRIYRELKFYTNNEFSFKEIYNRELFEQNAKV
                     LFEVVDRLQYFRFKYNAKAQFLGDFFEDMLESGFKQSEGQFFTPTPIAKFIVSAMPLK
                     EIVEEKIRNKEPKILPSVIDYACGSGHFITEAIEEIQQLIKTFNNKTHKDIASYKETT
                     KWAADFICGIEKDYRLARTSQVACFMHGDGDAKIIFGDGLEEHDKMPAKLYDVLVANP
                     PYAIKDFKKHLNKERLPKYELLQSISDDSDDIEAMFVERTKQLLKVGGYAGVFLPSPI
                     LTNTGIYMKAREILLKHFEITAVVEFGKNTFSATDVKTIVLFMKRRSDDFAKNCLYIA
                     EDLILGKDTERKEDFIDSKKLLENYTSLIGIDNTEYKTLLKRKPSAEFLKSEFYLNYK
                     KWFDDLTEVKNWKKGKNYEKLTKEQQEKHITNWFFDEVLEIEKEKFLYFLLSHRQIEK
                     PVKGNNKKSYKEFVPQQTLIIKSGKDNEIEKAFLGYTFSERRGYKGLDMKTDTNGREI
                     TKLFDKENLRNPDKVNTYIYKAFKEEPIAEISECLKEHIQPVNLIDCFNFEDEVFVKE
                     IDISLKAKINWEEIWGIERKKLKALDDFVHIEKGTSITKADVVTGVIPVIAGGKSAAY
                     YHNQSNYNGKVITISASGANSGYAAYHTGKIFASDCNVVYSKEEKEIPTYFFYCLLTA
                     IQGEIYLLQRGQAQPHVYGKDLKKILIPVLTKDKVSKVIKFVEDVEKNQYKHIKGNLN
                     LKDAESELNKVKREGVIKILTQ"
     gene            3974..4366
                     /locus_tag="P5335_09985"
     CDS             3974..4366
                     /locus_tag="P5335_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75250.1"
                     /translation="MENKQKHLEFAQGVVNRMGQNSFLIKGWSVTLVSALFALAAKDA
                     NQKFVIVAYFPTLVFWLLDSYFLYQERLFRKVYDHVRKETNTDFSLNTKQFDKGILDW
                     ASAALSKTILLFYGIIFLTLIIVMFYLK"
     gene            4375..4866
                     /locus_tag="P5335_09990"
     CDS             4375..4866
                     /locus_tag="P5335_09990"
                     /inference="COORDINATES: protein motif:HMM:NF020509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HRZ75251.1"
                     /translation="MARRAFFSFHYKNDVWRANQVRNSWVTKDDKEAAGFVDAAEFEK
                     VEKEGEDAIKRWINKQLEGTSVTVVLIGSETSNRPYVKYELQKSYEKGNGMLGIYIHK
                     CKDKDGNTCTKGSNQFGEIGKDAKGNPVYFSVDYPCYDWVDDDGYNNMGKWIEAAAKK
                     AGR"
     gene            4889..5146
                     /locus_tag="P5335_09995"
     CDS             4889..5146
                     /locus_tag="P5335_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75252.1"
                     /translation="MTKADIISAINGKVGSNYSIWTIGITDDPETRKSQHSNDGKSVK
                     YWSHWKTDSETIGRDVEKHFLDKGMKGDTGGGGKAGYVYVF"
BASE COUNT         2178 a          828 c         1049 g         1518 t
ORIGIN      
        1 cgttatgcac aagcggagaa aagacagcga cacagcaaaa tgacaatgaa actgactgtt
       61 attgactgaa agaaaaatga atttgccgac actcaagccg acacaatgtt tttaaaattt
      121 tttcccaccg cacatttttt taatacaatt ttattgcctg cccacattgg cacattgtct
      181 tttgccccga cacacgaagc caacccttcg gcaaaagcca aagagccaat tttgccgaca
      241 cacagaatta tgttgataac ttttataaaa tatttttgat ttatcaaaag caaattgact
      301 atctttgtca agacaaataa ttcaagaatg aaaactatag gtgaaataat tagaggtcac
      361 agagaagaaa agggaatgct tttaagacaa ttagcggcat tattagaaat tgactctgcc
      421 atacttagta aaattgaaag aggagagcga aaggcaacca aagaacagat aataaaaatt
      481 gctgacatac ttggcattaa acaaaatgat atgctgattc aatatttaag cgaaaaaatc
      541 gcttatgaaa ttgctgatga agaaacagca aagcaagttc taaaagtagc agaaaagaaa
      601 gttgattaca ttaaaaataa tcgcaaatga caacagaaaa catcaaaact ttaattaccc
      661 ttttgggttt tattcctgaa gataaaaaag ggaatatttg ggcaaaaaag tattctaatt
      721 atgataatta taccattacg ctagattttg agaaatcgtc tatcaactat ggagataaaa
      781 ttcagaaggg agacgataca acttctaatt tcagtagtga tgaaaatttt gttgtacttg
      841 aatgtattga cagacttcta gcaaaaggat ataaacccca agacctgaca cttgaaaaga
      901 aatggaaact cggacgaagt gctaagagcg gaaaagctga tataaacgtt actgatggaa
      961 aaacaggaaa gaccgttttg attattgaat gtaaaacatg gggtacagaa tttgaaaagg
     1021 aaaaagaaag aatgagagaa aatggaggac aattattttc atatttccag caagacaagg
     1081 caacccgctt tctttgcctt tatacttcac aaataacagc caatttgcta aaacaggaaa
     1141 agtttattga ttataaaact gcaatagtaa aagttgagga tacagtagaa gatattcgta
     1201 aacaagataa ccaaattgag catactttaa tttatcaaaa agcaactgat gttgcagaac
     1261 tggtaaaggt ttggaatcac aaaaataaaa agcagtttga agaaagaggg atttttgaag
     1321 aagaagttga gccatacaac cctggttttg ttccgcttcg tgttgctgac ttaaaagagt
     1381 ttaatcaaga cgatgcaaaa gcagagtact acaaatttga ggaaatatta agacacaata
     1441 atatttcaga ccgttcaaat gctttcaata gatttatttc attggtgttg gcaaaaattg
     1501 ttgatgaaga aaaaactgca aataccatta ctgaatttca aataaaaaaa ggcgttgaca
     1561 cttcggaaga attgattgag agattacaaa ccctttatgc aaaagcaatg agggattatt
     1621 taggcgaaac agtcatcaat tatacttttg aagagattga acaaataata agtcattttc
     1681 ctagacaaac ttcaaaagaa gatttaaaac ggatttatcg tgaattaaaa ttttacacca
     1741 acaacgaatt ttcatttaaa gagatttaca acagagaatt gtttgagcaa aacgccaaag
     1801 tattgtttga agtagttgat agattgcaat attttcgttt taaatacaat gctaaagccc
     1861 aattccttgg cgacttcttt gaagatatgt tagaaagcgg atttaaacaa agcgaagggc
     1921 agttttttac gcctacacca atagctaagt ttattgtttc cgcaatgcca ttgaaagaaa
     1981 tagttgaaga aaaaataaga aacaaagaac cgaaaatatt gccaagtgtt atagactatg
     2041 cttgtgggag cggacacttc ataacagaag ccattgaaga aattcagcaa ctcatcaaaa
     2101 cttttaacaa taagacacac aaagatattg cctcatacaa agaaacaact aaatgggcag
     2161 ccgatttcat ctgcggaatt gaaaaggatt accgattagc aagaacaagt caggtagctt
     2221 gctttatgca cggtgacggt gatgcaaaaa taattttcgg tgatggactt gaagaacacg
     2281 acaaaatgcc tgcaaaattg tatgatgtat tggttgcaaa tcctccttat gcgattaaag
     2341 attttaaaaa gcatctgaat aaagaacgac taccgaaata cgaattgtta caaagcatta
     2401 gtgatgacag tgatgatatt gaagctatgt ttgttgaacg aaccaaacaa ttgcttaaag
     2461 tcggaggtta tgcaggtgtt ttcttaccaa gccctattct tacgaatact ggtatatata
     2521 tgaaggcaag agaaattctt ttaaaacatt ttgaaatcac agcggttgtc gaatttggaa
     2581 aaaatacttt tagtgctaca gatgttaaga ccattgtttt atttatgaaa aggcggagcg
     2641 atgattttgc caaaaactgt ctatacattg ctgaagattt gattttgggt aaagacactg
     2701 aacgtaaaga ggattttatt gacagcaaaa aattattaga aaattacact tcactcattg
     2761 gtattgacaa tacagaatat aaaacacttt taaaacgcaa accatcggct gaatttctaa
     2821 aatcagaatt ttatctgaat tataaaaaat ggtttgatga tttaaccgaa gtaaagaatt
     2881 ggaaaaaggg taagaattac gaaaaactca caaaagaaca acaggagaaa catattacca
     2941 actggttttt tgatgaagta ctagaaattg aaaaagaaaa attcttatac ttcctccttt
     3001 cacacagaca gattgaaaaa cctgtaaaag gcaacaataa aaagtcgtat aaggaatttg
     3061 ttccgcaaca aaccttgatt attaaatctg gtaaagataa cgaaatcgaa aaagcatttc
     3121 tcggatatac ttttagtgaa cgaaggggct ataaggggtt ggacatgaaa acagatacta
     3181 atggtagaga aataaccaaa ctatttgata aggaaaattt gcgaaatcct gataaagtaa
     3241 atacttacat ttataaagca tttaaggagg agccaatagc agaaatttca gagtgtttga
     3301 aagaacatat tcaacctgtt aacttaattg attgcttcaa ttttgaggat gaggtatttg
     3361 ttaaggaaat tgacatttct ttaaaagcta aaatcaactg ggaagaaatt tggggtattg
     3421 aaaggaaaaa actaaaggca ctggatgatt ttgtgcatat agagaaagga acatctatta
     3481 caaaagcaga tgtagtaacc ggtgtaatac cagttattgc aggcggaaaa tctgcagcat
     3541 attatcacaa tcaaagcaat tataacggaa aagtcattac aatcagtgca tcaggtgcaa
     3601 attcaggata tgccgcatac catactggaa aaatctttgc ttcagattgt aatgtagtat
     3661 actcaaaaga agaaaaagaa atcccaactt attttttcta ctgtttgctt actgcaattc
     3721 agggggaaat ctatttgctt caaagaggtc aagcacagcc acatgtttat ggcaaggatt
     3781 taaagaaaat actcatacct gttcttacaa aagataaagt ttccaaagtt ataaagtttg
     3841 ttgaagatgt tgagaaaaat caatataaac acatcaaagg aaatctaaat cttaaagatg
     3901 ctgaaagtga attaaacaaa gtaaaaagag aaggagtaat taaaattcta actcaataaa
     3961 aaattttaca agtatggaga ataagcaaaa acatcttgaa tttgcacaag gagtggtaaa
     4021 ccgaatgggg caaaattcat ttttaattaa gggatggtct gttacgcttg tatctgcact
     4081 gtttgcatta gcggcaaaag atgcaaacca aaaatttgta attgtagcat actttccgac
     4141 acttgttttt tggttacttg atagttattt tctttaccag gaacgattat ttagaaaagt
     4201 ttatgaccac gtacgcaaag aaactaacac tgacttttca ttgaatacaa aacaatttga
     4261 taagggtatt ttggattggg ctagtgctgc actttcaaaa acaatacttc ttttttacgg
     4321 gataatattt ttaacactaa taattgtaat gttttactta aaataaaaat caaaatggca
     4381 agaagagcat tttttagctt tcactacaaa aatgatgtat ggagagcaaa tcaagtaaga
     4441 aatagttggg tcactaaaga tgacaaagaa gctgctggtt ttgttgatgc agcagaattt
     4501 gaaaaagttg aaaaggaagg agaagacgca ataaaaagat ggataaataa gcaacttgaa
     4561 ggaacatctg taactgttgt gttaattggt tccgaaacga gcaacagacc atatgtaaaa
     4621 tatgagttgc aaaagagcta tgagaaaggc aatggtatgt tgggcatcta tattcacaaa
     4681 tgtaaagaca aggatggaaa tacatgtaca aagggttcaa atcaatttgg agaaattggg
     4741 aaagatgcaa aaggaaatcc tgtttatttt agcgttgact atccttgtta tgattgggta
     4801 gatgatgacg gttataacaa catgggtaaa tggattgaag cagcagcaaa gaaagcagga
     4861 agataaaata ttcaaaaaat ttaatattat gacaaaagca gacatcattt cagcaatcaa
     4921 cgggaaagtt ggcagtaatt attcaatttg gactattggt attacagatg acccagaaac
     4981 aagaaaatcg caacactcca atgacggcaa atctgttaag tattggagtc actggaaaac
     5041 cgacagtgaa accatcggac gagacgttga aaaacatttc cttgacaagg gaatgaaagg
     5101 cgacactggt ggaggcggaa aagcaggtta tgtttatgtt ttttaggcag acacacaaaa
     5161 gcatacacat tttgcagccg ctaatccaaa cacacgagcc aaagctgcaa aaagagtatg
     5221 cttttcatca gcccgagaaa aattgtatta ataaaatctc cttcccttca aaaaatttta
     5281 aaaacgctga cacacaagcc aacgactgat aataaattat gacaatacca gcagaaatga
     5341 aacgacataa ctacttgaca gacaagaacc gccagtgcat aacaggcggt atattttatt
     5401 gccgagatag tgcgggtttc agcgtttctg catctaataa actttcgtgt aacttgacag
     5461 gacagtgctt cgaaatcggc aacaaaaaca taccgccaat ccgttaccat gcatatcaaa
     5521 aaaaacggat aaagacgaat gagtagagta gattcggtcg ttctttgaat cga
//
LOCUS       DAOTCW010000145         5526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_11007_length_5526_cov_35.239262, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000145 DAOTCW010000000
VERSION     DAOTCW010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11007_length_5526_cov_35.239262"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="P5335_10000"
     CDS             <1..1481
                     /locus_tag="P5335_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRZ75253.1"
                     /translation="FLFSQKNEEVGVLFQKSEETLFRNPEQCLKITNHIVHNSTTPNE
                     LAQAYLLLSKSYFTSGKFMLAAENILLANQQAEQSSEKDIYIESLLFSSEIYNFLGLY
                     EVANQHSEKAKMVAQKDKKLEERVKTYLYFNTPDNKHKRSDSNLTNELNYSFLSKGTI
                     QTQLATEYLSQSKLDSALVYFQKSIDNNKAINVGSYWEMITALHFSNYFFAKKEYQNA
                     IEQLNLAIEKGKLFSNDYFQESIYEKLSKNYLVTNNIIKYRELNQKAMTSGSNVSTEL
                     TLATNYVIEQLHKVNEEKIDKARTFQNNINWILATIASVLLLGWLFMKWLFATRIQYM
                     TDIISYLKLIHNSETKIESNERVLLKSIPKPKEIEELLLTKLTKFETGEKFLNKDMSL
                     ALLASQLDTNTKYLSEVINKYKGKNFNVYINELRINYIVSKLKTNPMYLNYKVSYLAD
                     ECGFSSHSSFSTVFKNCTGIKPNVFIQFLMKDRNENNKSSAA"
     assembly_gap    1493..1592
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1593..2300
                     /locus_tag="P5335_10005"
     CDS             <1593..2300
                     /locus_tag="P5335_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75254.1"
                     /translation="KLIGFNYAGRGFIYREQMSCDIAQNYFNKSLYYFKRNLKAPLMN
                     ANLSIISYNKGNCFIASNQIDSAKISFNQAIDFSKKIEANSLEAFAYKGLAEVHTSEG
                     NHLLAIKQLTDALKIAEQVGDLVLNEGIYKGLADNYLAVNNHELYHMYVLKYNEIKKQ
                     THDAETKTINQLIVQDDKEATIKIEQQKASALYYRIPLISLIVLLIGLCFFEFFRYKK
                     KYKKLKTDKINLEKQSS"
     gene            2455..>3449
                     /locus_tag="P5335_10010"
     CDS             2455..>3449
                     /locus_tag="P5335_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRZ75255.1"
                     /translation="MRKITLLAFFAFSLSATNLFGQFLVKDSFFETTGVSNVGTVSGY
                     ESQTGPYIIWNPDANTTQYIGGVAPGNGVGGSAEFSNDGNFLSGTNMIDLPISTDWQR
                     NILTDYNYIFRGIEFPEAQDLFGYAVGQSLTYNGNGIVLRTTNGGQTWGAMWVDDQQR
                     GLEAMSFPTFGTGYVGGFNHYFAKTENAGWDWTELNPAGTDNVYKYTAIEFADDLNGV
                     VGAQLEEGAALYITSDGGQTWTTASGLAAIPYKINYVGEGTYFLVTNGGDIQKSIDFG
                     ATWTTVYADANTLFLGIRFLNSMIGIATAETNIVKTMDGGATWTSSPIAPGVTDGV"
     assembly_gap    3450..3549
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3550..4599
                     /locus_tag="P5335_10015"
     CDS             <3550..4599
                     /locus_tag="P5335_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ75256.1"
                     /translation="QWTDLGSLGFQVDGTTSGGFNISGDGLTVVGNSWANPSNGNGTS
                     PYAHAVAWSATEGLMDLGSMYASINRSSRANAVNNDASVVVGYQDFNGPWKSAVWRKN
                     PEGGYFPNEYILIDPAGNPTDEYNQVGECSAVSGDGNWIGGYGDYANADEPWIWSEAT
                     GVINLGTLGGVGRVSAINNDGSVVIGWFDNGFWDPKIPFIWTPTEGLQNLNTYITETL
                     GFTMSYSPIHVPSNMSDNGKYIVGWGYDPTVGEWGESFNFRLQLPDGLGVNEVALNNA
                     VNVYPNPVKDILNISSKELINNVQVFNVSGQQILTEKAMNAITKIDLSSLSSGVYFVK
                     TSSVNASKTIKVIKQ"
     gene            complement(4697..5047)
                     /locus_tag="P5335_10020"
     CDS             complement(4697..5047)
                     /locus_tag="P5335_10020"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRZ75257.1"
                     /translation="MIEINVIREILFLLPEVIEEQHLKLISFRVRKEVFVTYNYEQSQ
                     VCLKLSPAEQRIFHSKHELSFYPVPNSWGRQGWTHVMLDIVEKRVLKDAIFSAYSANA
                     PEKLLEQIKPKDKE"
     gene            complement(5099..>5526)
                     /locus_tag="P5335_10025"
     CDS             complement(5099..>5526)
                     /locus_tag="P5335_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75258.1"
                     /translation="NIPTMQQEKDHLKKQIDQLGIVLRKVLSDLLGIKQQGVQQDVVE
                     TTNKALKSEIDLDLNEFIALPDDSFIDTLISKKGFNNENLDYFTDILISIAERYPDDE
                     AMKKSLYQKCLLIYTFQEKSASTYSFGRQMKTDRLKKLL"
BASE COUNT         1817 a          865 c          954 g         1690 t
ORIGIN      
        1 cttttttatt ttctcaaaaa aatgaagaag taggtgttct ttttcaaaaa tcggaagaaa
       61 ctttgttcag aaatccggaa caatgtttaa aaatcacaaa ccatattgtt cataattcaa
      121 ccacaccaaa tgaattagct caagcctatt tattactttc taaaagctat ttcacttccg
      181 gaaaatttat gcttgcagct gaaaatatcc ttttagcaaa tcaacaggca gaacaatctt
      241 ctgaaaaaga tatttatatt gagtctcttt tgttttcctc agaaatttat aattttttag
      301 gactatatga agtggctaat caacatagtg agaaagcaaa aatggttgct caaaaagata
      361 aaaaattaga ggaaagagta aaaacatatt tatatttcaa cacaccagat aataaacata
      421 aaaggtcaga tagtaacctt actaatgaat tgaactattc ctttttatct aaaggaacaa
      481 ttcaaactca acttgccacg gaatacctta gccaatcaaa acttgattcc gctttggttt
      541 attttcaaaa aagtatcgat aacaacaaag ctattaatgt agggagttat tgggaaatga
      601 ttacggcctt gcatttcagc aactatttct ttgccaaaaa agagtaccaa aatgcaattg
      661 aacaattaaa cctggctatt gagaaaggga agttgttttc aaatgattat tttcaagaat
      721 ctatttatga aaaactatcc aaaaactatt tagtcacaaa taatataatt aaataccgag
      781 aattaaatca aaaagcaatg acttctggca gtaatgtttc tacagaattg accttagcta
      841 ctaattatgt aatagagcaa ttgcataaag taaatgaaga aaaaattgac aaagcaagaa
      901 cttttcaaaa caatataaat tggattttag ccactattgc cagtgttttg cttttgggat
      961 ggctctttat gaaatggctt tttgcaacta gaattcaata catgactgac attatcagtt
     1021 atttaaagct tatccataat tctgaaacta aaatagaatc caatgaaaga gtattgttaa
     1081 agagtattcc taaaccaaag gaaatcgaag agcttctgct aacaaaatta accaagtttg
     1141 aaacgggaga aaagttttta aacaaagata tgtcgttagc tcttttagcc tcacaattgg
     1201 ataccaacac aaaatacctt tcggaagtaa ttaataaata caaaggaaaa aatttcaatg
     1261 tctatattaa tgaattgcga atcaattaca tcgtttcaaa actcaaaacc aatccgatgt
     1321 atttgaatta taaagtaagt tatttagctg atgaatgtgg cttttcatct cacagtagct
     1381 tttcaaccgt atttaaaaac tgtaccggaa ttaaacccaa tgtttttatt caatttttaa
     1441 tgaaagacag aaatgaaaac aataaaagca gtgctgccta aataaatgaa aannnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaattaat cggatttaat tatgccggaa
     1621 gaggctttat ttacagagaa caaatgagct gtgatattgc tcagaattat ttcaataaat
     1681 cgctctatta tttcaaaagg aacctcaaag cacctctcat gaatgctaat ttgagtatca
     1741 tttcttataa caaaggaaat tgcttcattg cttccaatca aatagattcg gctaaaatca
     1801 gttttaacca agcgattgat ttttcaaaaa aaattgaagc caatagtctg gaagcttttg
     1861 cttataaagg cttagcagaa gtacatacta gtgaaggaaa tcatttgctg gctattaaac
     1921 agctcacaga tgctttaaaa attgctgaac aggtgggaga ccttgttctt aatgaaggaa
     1981 tctacaaagg gttagccgac aattacctcg ccgtgaacaa ccatgaattg tatcatatgt
     2041 atgtcttgaa atacaatgaa attaaaaagc aaacacacga tgcggaaaca aaaacaatta
     2101 accagttgat tgttcaggat gataaagaag ctacgattaa aatcgaacag caaaaagcct
     2161 ctgctttata ttacagaatt ccgcttatca gtttaatagt tttactgata ggcctctgct
     2221 tttttgaatt tttcagatat aaaaagaagt ataaaaaact caaaacagac aaaattaatc
     2281 tggaaaaaca gtcttcctga cgaatgtttt aaatccaaac tttttttgtg ctaaataatt
     2341 ataattcaat attttaaaag ttatttttac tttcaaaatt catgaatttc aaaagacttc
     2401 tttatttttg tattgtataa agcaatattt tagccttatc aaaaaataaa caacatgaga
     2461 aaaattactt tacttgcttt ttttgcattt tctttaagtg caactaattt atttggccag
     2521 tttctggtta aagattcatt ttttgaaact accggagttt caaacgtagg gacagtttcc
     2581 ggatatgaat cgcaaaccgg accttatatc atttggaatc ctgatgcaaa tacaacacaa
     2641 tacatcggtg gtgtagctcc cggaaatgga gttggaggtt ctgctgagtt ttcaaatgac
     2701 ggtaattttc tttccggaac caacatgatt gatttgccta tatcgactga ttggcaacga
     2761 aacattttaa cggattacaa ttatattttt agaggtattg aatttccgga agctcaggat
     2821 ttattcggct atgcggtggg acaatcacta acatataatg gtaatggaat tgttttaaga
     2881 actacaaacg gtggacaaac atggggagcc atgtgggttg atgaccaaca gagaggtttg
     2941 gaagccatga gttttccaac ttttggaacg ggatatgtag gtggttttaa tcattacttt
     3001 gctaaaactg aaaatgcagg ctgggattgg acagaattaa atccagccgg aactgacaat
     3061 gtatataagt acacggccat cgaatttgct gacgatttaa atggagtggt aggtgcacag
     3121 ctagaagagg gtgctgccct atacattacg tctgatggtg gtcaaacgtg gacaaccgct
     3181 tccggtttag ccgctattcc ttacaaaatt aattatgttg gtgagggcac ttacttctta
     3241 gttactaacg gtggtgatat tcaaaaatca attgattttg gggctacgtg gacaacggtt
     3301 tatgctgatg caaacacctt atttttggga atcagatttt taaattccat gattggaata
     3361 gcaacggctg aaacaaacat tgttaaaacg atggatggtg gagcaacgtg gacttcttct
     3421 ccaattgcac caggcgttac ggatggtgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnc aatggactga tttaggaagt cttggttttc aagtggacgg aacaaccagt
     3601 ggtggattca acatttccgg tgacggtttg accgtagtag gaaattcttg ggcaaaccct
     3661 tctaacggta acggaacatc gccttatgct catgcagtgg catggtctgc aacggaaggt
     3721 ttaatggatt taggcagtat gtatgcttct atcaacagaa gttccagagc aaatgccgta
     3781 aataacgatg cttctgttgt ggttggttac caagatttta atggaccttg gaagtcggct
     3841 gtatggcgta aaaatcctga aggtggttat ttcccgaacg agtatatatt aatagaccct
     3901 gccggaaacc caacggatga atacaatcaa gttggtgaat gttctgccgt ttccggtgac
     3961 ggtaactgga ttggcggata tggtgactat gctaatgctg atgaaccttg gatttggagt
     4021 gaagctaccg gtgtaattaa tttaggtacc cttggtggtg taggtagagt ttcggccata
     4081 aacaacgatg gtagtgtagt aatcggttgg tttgacaatg gtttttggga tccaaaaatt
     4141 ccatttattt ggactcctac ggaaggttta caaaatttaa acacctatat cacggaaacc
     4201 ttaggattca ccatgagtta tagtcctatt catgttccta gcaatatgtc tgataatgga
     4261 aaatatatag taggctgggg ttatgatcct accgttggtg aatggggtga atcatttaac
     4321 ttccgtttac aattgcctga tggcttaggg gttaatgaag tcgctctaaa caatgctgta
     4381 aacgtttatc caaacccggt aaaagacata ctgaatattt cttcaaaaga attaatcaac
     4441 aatgtgcaag tattcaacgt aagcggtcaa caaattttga ctgaaaaagc aatgaatgca
     4501 atcactaaaa ttgatttgtc ttctttaagc agtggagtat attttgtaaa aacaagctct
     4561 gtaaatgcaa gcaaaacaat caaagtgatt aaacaataag ttgaaagtaa gtttttttat
     4621 tatatagttt ttattcaaag cgaaaatccc gaagccaaca cttcgggatt tttatttttt
     4681 tatttctcaa cctagtctat tctttgtctt tcggtttaat ttgttccaat aacttttcag
     4741 gggcatttgc actgtaagca ctgaaaattg catctttcaa aacacgcttt tccacgatat
     4801 ccaacataac atgagtccat ccttgacggc cccaactgtt cggaaccgga taaaatgata
     4861 gctcgtgctt agaatgaaaa atacgttgct cagccggtga aagttttaga caaacttgag
     4921 attgttcgta gttataagta acaaaaacct cctttcttac cctgaaagaa attaatttaa
     4981 gatgttgttc ttcaattact tcaggtagta aaaataaaat ttcacgtata acgttgatct
     5041 caatcaaaac cgttttgatg ttttataatt atagtaccaa agtaacttaa aaatttgttt
     5101 ataacaactt ttttaatcga tctgttttca tttgtcggcc aaaagagtaa gtagaagctg
     5161 atttttcttg aaaagtatag atgaggagac acttttgata caaacttttt ttcattgctt
     5221 catcgtcagg atagcgttct gcaatagaaa taagaatatc tgtaaaataa tccagatttt
     5281 cattattaaa cccctttttg ctaataagtg tatcgataaa actatcatca ggaagagcaa
     5341 taaactcatt caaatctaag tcaatttcac tcttcaatgc cttattggtc gtttcaacaa
     5401 catcttgctg cacaccttgc tgttttatac ccagtaagtc cgaaagcact tttcgcaaca
     5461 caataccaag ttggtcaatt tgttttttta gatgatcttt ttcttgttgc atggtaggaa
     5521 tatttt
//
LOCUS       DAOTCW010000146         5518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_11033_length_5518_cov_47.823906, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000146 DAOTCW010000000
VERSION     DAOTCW010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11033_length_5518_cov_47.823906"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /gene="gldK"
                     /locus_tag="P5335_10030"
     CDS             complement(<1..1193)
                     /gene="gldK"
                     /locus_tag="P5335_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldK"
                     /protein_id="HRZ75259.1"
                     /translation="MKKFIALSTILVVLVSCGSSDKGELVGVRGKKWNPEKPYGMSLI
                     PGGAYIMGKSDDDPANVQDAPTKTVTVRSFYMDETEITNSEYRQYVEWVKDSVTRMKL
                     AVLADELGQTSATAGEKGGGKKGGGSIGDYAFSDADPEKMTPYDKYMYENYYSIGTDD
                     DPYAGRRLNRKVKLINETSKYPDEYYVEVMDSMYLPVAESFNGLRTIDVNKLEFSYSW
                     MDIQAAAKAKTGKRSDFIRKEKVKIYPDTTSWIKDFAYSYNEPMHNDYFWHQAYSDYP
                     VVGVSWKQAKAFCAWRTLNKNIYIKSKKKKQDLINTFRLPTEAEWEYAARGGLQSATY
                     PWGSNYAKNDRGCYLANFKPNRGDYAADEALYTVEAKSYEPNNYNLYNMAGNVSEWTD
                     SSYDAA"
     gene            complement(1398..>2195)
                     /locus_tag="P5335_10035"
     CDS             complement(1398..>2195)
                     /locus_tag="P5335_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HRZ75260.1"
                     /translation="PIIIGGSQDLTYAMYRGYDQLDQMVNLVAIDSKFDFGKEEVLNT
                     SESFLSKIIINEPTNLFNYSNVGFQTYFNSQEEIDLIEKLYFDCFRLGEVSASPAIAE
                     PVFRDCDLVSLDLTAVKSSDSGNFNIFTPNGFNGKEICALSRYAGLSDKVTSFGIFNQ
                     HNSKNESVLIAQIIWYFIEGYNYRSLESPNQNTFLKYIVPIDDLELVFFKSPKTERWW
                     IEIPFFTNVNNKLKKNTLLPCTHEDYLRACDQEIPDRWWKVQRKNLI"
     assembly_gap    2196..2295
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2325..4841)
                     /gene="topA"
                     /locus_tag="P5335_10040"
     CDS             complement(2325..4841)
                     /gene="topA"
                     /locus_tag="P5335_10040"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRZ75261.1"
                     /translation="MAKNLVIVESPAKAKTIEKFLGSDFQVESSYGHIADLPSKEIGV
                     DVENDFKPKYEVSPDKKALVSKLRTLSKSAEMVWLASDEDREGEAISWHLAEELKLDP
                     KKTKRIVFHEITKSAIQKAIENPRGIDYNLVNAQQARRVLDRLVGYELSPVLWKKVKG
                     GLSAGRVQSVSVRLIVEREREIQDFKAVASYSVSAEFSTEAGKIVKAKLAKNFSTKKE
                     AEDFLQKNIGSNYKVSDLETKPTKKTPAAPFTTSTLQQEAARKLYLPVGITMQIAQRL
                     YEAGHITYMRTDSVNLSQEAMGAAQAEITRSYGKEFSKPRNFTNKSKGAQEAHEAIRP
                     TDMSKHTLNIDRDQARLYDLIWKRTLASQMSDAELERTNVKIEANNHSEHFSATGEVI
                     KFEGFLKVYLEGSDEDEEEQEGMLPAMKVNEKLINNSITATERFSRPASRYTEASLVK
                     KLEELGIGRPSTYAPTISTIINRNYVEKGSFEGQERKYNQITLKGNDIKSVILSENSG
                     SDKGKLVPTDIGIIVNDFLVKNFATILDYNFTAKVEQDFDEIAEGNEDWTKMMRDFYN
                     HFHPNVLDVEENADRESGERILGNDPKTGKPVSVRLGKFGPMAQIGDIDDENKQFASL
                     GTEQNIGTITLEDALNLFLLPKNLGIYKGEEVEVNNGRFGPYVRFGKTFISLPKGEDP
                     LDVSLERAQELIDEKNQADAPIGTYEGLPIQKGVGRFGPFIKWNGMFINVSKKYNFDK
                     LAQADLNELIEDKQQKEIDKVIHDWKAEGIKVEKARWGRSIITKGKIKIELSKDIDAE
                     KLTLMQVQELIEKKSPAKKTTAKKTATKKAVTKKATIKKK"
     gene            5019..>5518
                     /locus_tag="P5335_10045"
     CDS             5019..>5518
                     /locus_tag="P5335_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989268.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRZ75262.1"
                     /translation="MEKTIEENKQGTSLVLEQKESNKKKLFIESYGCAMNFSDSEIVA
                     SILNEQGYNTTQNLEEADLVLVNTCSIRDKAEQTIRKRLEKYNAVKKINPSMKVGVLG
                     CMAERLKSQFLEEEKIVDMVVGPDAYKDIPNLLKEVEEGRDAINVILSKDETYGDISP
                     VRLNSNG"
BASE COUNT         1625 a         1035 c          793 g         1965 t
ORIGIN      
        1 gctgcatcat aagaagagtc tgtccattct gatacgttac ctgccatatt atataagtta
       61 taattatttg gctcatacga ttttgcttct acagtatata acgcttcatc agcagcatag
      121 tcacctctgt ttggtttgaa gttagctaaa taacaacctc tgtcattttt agcataatta
      181 cttccccatg gataagttgc tgattgaaga ccgcctctag cagcatattc ccattcggct
      241 tcagttggta aacgaaacgt attaattaaa tcttgtttct ttttcttaga tttaatgtaa
      301 atatttttat ttaatgttct ccacgcacaa aatgcttttg cttgtttcca agaaacacct
      361 actacaggat aatcactgta agcttggtgc cagaaataat cattgtgcat tggttcatta
      421 taggaataag caaaatcttt aatccaagaa gttgtgtcag gatagatttt tactttttct
      481 tttctaataa aatcagatct tttgccagtt tttgctttag ccgctgcttg aatatccatc
      541 catgaataac tgaattctaa tttatttaca tcgatagtac gcaagccatt gaatgattca
      601 gctaccggta aatacattga atccattact tcaacataat attcatcagg atattttgaa
      661 gtttcattaa ttaatttcac ttttctgttc aatcttcttc ctgcataagg atcatcatca
      721 gttccaatac tgtaataatt ctcatacatg tatttgtcat aaggagtcat tttttcaggg
      781 tctgcatctg aaaacgcata atccccaatg cttcctccac cttttttacc accacctttt
      841 tcgccggcag tagcagatgt ttgacctaat tcatcagcta aaacagctaa tttcattctg
      901 gtcacagaat cttttaccca ttccacatat tgtctgtatt cactatttgt gatttctgtt
      961 tcatccatat agaatgaacg aacagttaca gttttagttg gtgcatcttg cacattggcg
     1021 ggatcatcat ccgattttcc cataatgtaa gcaccaccag gaattaatga cattccgtaa
     1081 ggtttctccg gattccactt tttacctcga actcctacaa gctctccttt gtcgctggaa
     1141 ccacaactaa ccaatacaac taaaatagtt gataatgcga taaacttctt catataaatt
     1201 tgggttatca tgtactcgaa tttttaaggc cgtaaaccta tttataattt ttttaaaatg
     1261 caattatttc ttctactttt tattttctag tcaaaactaa taagaaactt attattcgaa
     1321 aagaaaaaaa tcgataaaat acacaaatta tcgacgaaaa gcactttttt gtaaaatatg
     1381 taatacttat ctttcaatca aataagattc tttctttgaa ctttccacca tctatcaggg
     1441 atttcttggt cgcaagctcg caaataatct tcatgtgtac atggcaataa cgtatttttt
     1501 tttaatttat tattaacatt tgtaaaaaat ggtatttcaa tccaccatct ttccgtttta
     1561 ggacttttaa aaaacacaag ttctaaatca tcgataggaa ctatatattt taaaaaagta
     1621 ttttgatttg gagattctaa agacctataa ttgtatcctt ctataaaata ccaaataatt
     1681 tgtgctatta aaacactttc attttttgaa ttatgctgat taaaaatccc aaatgatgtt
     1741 accttatcgc ttaatccggc atatcgggat aaagcacaaa tttcttttcc attaaaacca
     1801 ttcggtgtaa atatattgaa atttcctgaa tctgatgatt ttacagctgt taaatctaaa
     1861 gaaactaaat cacaatcgcg aaagaccggc tctgcaattg ctggacttgc tgaaacttct
     1921 cctaaacgaa aacaatcaaa atataatttt tcgattaaat ctatttcctc ttgcgaatta
     1981 aaataggttt gaaatcctac attactataa ttaaataaat ttgttggttc attaatgata
     2041 atttttgaga ggaaagattc ggatgtgttt aaaacctcct cttttccaaa atcaaattta
     2101 ctatcaattg ctactaaatt taccatttga tctaactgat cataacctct atacatagca
     2161 taagtcaagt cttgagaacc tcctattatt attggnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnaccgg ttctaaaaaa tcaaatgcca tgaattattt ctttttaatt
     2341 gttgcctttt tggttactgc ttttttagtc gcagtctttt tggctgttgt ttttttagcg
     2401 ggagattttt tctcaattaa ttcttgaact tgcatcaacg tcaatttttc agcatcaata
     2461 tctttactca attcaatttt tattttccct ttagtaataa ttgatcgacc ccaacgtgct
     2521 ttctctacct taattccttc agctttccaa tcatggatta ctttatcaat ttctttttgt
     2581 tgtttatcct caatcaactc atttaaatca gcttgtgcta atttatcaaa attgtatttt
     2641 ttacttacgt tgataaacat cccgttccat ttaataaaag gtccgaaacg ccccactcct
     2701 ttttgaatag gcaatccttc atacgtcccg ataggagcat ctgcttggtt tttttcatca
     2761 attaattcct gagctctttc caaagagacg tctaatgggt cttccccttt tggcaaggaa
     2821 ataaatgttt ttccgaaacg aacatagggt ccgaaacgac cattattcac ttctacttct
     2881 tctcctttat atatacccaa atttttgggt aacaaaaaca agttcaatgc atcttccagg
     2941 gtaatcgttc cgatgttctg ttctgttcct aaactggcaa attgtttatt ttcatcatcg
     3001 atatcaccaa tctgtgccat tggaccaaac ttccctaaac gaacagaaac cggtttacct
     3061 gtttttggat cattccctaa aattctttct ccgctttctc tatccgcatt ttcttctaca
     3121 tctaatacat tcggatgaaa atgattgtag aaatcacgca tcatttttgt ccaatcttca
     3181 tttccttcgg caatttcatc aaaatcctgt tccactttag cagtaaaatt ataatctaaa
     3241 atggtagcaa aatttttaac caaaaagtca tttacaatta ttccaatatc tgttggtact
     3301 aatttacctt tatctgaacc actattttca gaaagaatta ctgatttaat atcgtttcct
     3361 tttaatgtta tttggttgta ttttcgttct tgtccttcaa aactaccttt ctcaacataa
     3421 tttctgttga taatggtaga aattgttgga gcatacgttg acggacgacc aataccaagt
     3481 tcttctaatt tcttcactaa ggaagcttct gtatatcgac tggccggtct tgaaaaacgc
     3541 tcagttgccg tgatagaatt attaattaat ttttcattca ctttcatggc cggcaacatt
     3601 ccttcttgtt cttcttcgtc ttcgtcgcta ccttctaaat aaacttttaa gaaaccttca
     3661 aacttgatta cttctccggt tgcagaaaaa tgttcggaat gattattcgc ttcaatttta
     3721 acatttgtac gttccaattc ggcatcactc atttgagagg ccaaggttcg tttccaaatt
     3781 aaatcataca accttgcttg atctctgtct atatttaagg tatgttttga catatctgtt
     3841 ggacgaatag cttcgtgtgc ctcttgagct cctttggatt tgtttgtaaa atttctaggc
     3901 ttgctaaatt cttttccata ggaacgggta atctcagctt gagctgcacc cattgcctct
     3961 tgagaaaggt tcacactatc cgttctcata taagtaatat gtccggcttc atataaacgt
     4021 tgagctattt gcatcgttat tccaacaggt aagtacaatt tccgagcggc ttcctgttgc
     4081 aaagttgatg ttgtaaaagg agctgccggt gtttttttag ttggtttagt ttctaaatcg
     4141 gaaactttat aattagaacc tatatttttt tgaagaaaat cctctgcttc ttttttagtc
     4201 gagaagtttt tagccaactt tgcttttact atttttcctg cctcggttga aaattcagct
     4261 gaaacgctgt atgatgcaac tgctttaaaa tcctgaattt cacgttcccg ttccacaata
     4321 agtcttactg aaactgattg cactcttccg gcagaaagtc cgccttttac ttttttccaa
     4381 agtaccggcg acaattcgta acctactaat cgatctaaaa ctctacgagc ttgttgagca
     4441 tttactaaat tataatctat ccctcgtgga ttttcaattg ctttttgaat ggcagattta
     4501 gtaatttcat gaaaaacaat acgtttggtt ttttttggat ctagttttaa ttcttcggct
     4561 aaatgccaag aaatagcttc cccctcacgg tcttcatcac tagctaacca taccatttca
     4621 gcacttttag ataacgttct cagtttgctt accaacgcct ttttatcggg agatacttca
     4681 tatttaggtt taaaatcatt ctcgacatca actccaattt ctttcgaagg aaggtcggca
     4741 atgtggccat aactggactc aacctgaaaa tcacttccca aaaatttttc gatggttttt
     4801 gcttttgcag gtgactcaac gattactaaa ttctttgcca tgtacgtttt tgtttggtga
     4861 caaaagtatg gttttttttt aattattaat ttttgaaaaa taaaaaccca attttaactc
     4921 atcgttacaa tcaaattttt gttaaactac aactgccatc ttgtcagatt ttgattttta
     4981 gtattatctt tgctaacaga tttcagacaa atttgattat ggagaagaca attgaagaaa
     5041 acaagcaagg aacaagctta gttttagagc aaaaagaaag caataagaaa aaacttttta
     5101 tagaaagtta cggttgtgcg atgaattttt cggacagtga aattgtagct tctatattga
     5161 atgaacaagg atataataca acccaaaatc ttgaagaagc tgatttggtt ttagtcaaca
     5221 cctgttctat ccgagataaa gctgagcaaa ccattcgcaa acgattagaa aaatacaatg
     5281 ccgttaaaaa aataaaccca agcatgaaag tgggtgtgtt gggctgcatg gcggaacgat
     5341 taaaaagtca gtttttagaa gaagaaaaga ttgtagacat ggttgttgga ccagatgcct
     5401 acaaagatat tcctaattta ttgaaggaag tagaagaagg ccgagatgct atcaatgtaa
     5461 ttctatcgaa agatgaaact tatggtgata tttctcctgt tcgactcaat agtaatgg
//
LOCUS       DAOTCW010000147         5491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_11114_length_5491_cov_43.739330, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000147 DAOTCW010000000
VERSION     DAOTCW010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11114_length_5491_cov_43.739330"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="P5335_10050"
     CDS             complement(<1..1235)
                     /locus_tag="P5335_10050"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRZ75263.1"
                     /translation="MNIPNTNLPRIVIIGGGFAGIALAKKLKNQRVQVVLLDKHNYHT
                     FQPLLYQVATGGLEAGSIAYPIRKVVQEYQNFYFRLGFVNEIDTKNQKVKADIGELRY
                     DYLVLATGSKTNFFGNKEIERNSVAMKTIPQSLNIRSLILENFEQALLTNDIHDRHSL
                     ISFVLVGGGPTGVELAGALAEMKKAILPKDYPDLDVRKMQINLIQSSDRILDSMSEKS
                     SDAAEDFLIKLGVNVWKNVRVTGYDGSTVTTNTDLTFETATVIWTAGVQGALPSGIDV
                     NAIIPRADRVKVNEFNQVEGYDNIFAVGDIAAMISEENPKGHPMMAQPAMQQGKWLGE
                     NIVLKINKQPLKPFVYNDKGSMATIGRNKAVVDLPKFHFSGVFAWFVWIFVHLFSLIG
                     FKNKAVVLTNWIYNYIRLDR"
     gene            1620..2114
                     /locus_tag="P5335_10055"
     CDS             1620..2114
                     /locus_tag="P5335_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRZ75264.1"
                     /translation="MSNSLEQSFVKQLKENQNIIHKICRLYTSGEDAHNDLFQEITIQ
                     LWKAFPKFRGESKFSTWAYRVALNTAITLYRKSTRTVATTDMEGKKQFVAPEDYNFEE
                     EEQIKLLYKAVQQLNDIEKALVFMYLEDKDYEEIAETLGISEVNARVKMNRIKGKLKK
                     IVNP"
     gene            2119..2760
                     /locus_tag="P5335_10060"
     CDS             2119..2760
                     /locus_tag="P5335_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75265.1"
                     /translation="MTEELDLLKKAWKKDAHSYEQVTENQIYAMIHKRSSSIVKWILM
                     ISIAEFIIWSGIGFFTVDDNYLKTLEIYHIDKLFKIITGFNYAIVIVFIYLFYKNFKT
                     ISTNETVKKLMSSIIKTRKTVQYYIWYNLTMFAIIFIIVVISQITYDPNLNSVIENTK
                     EMNPQTFWVIIGLIYFALFAVTFGLFWLFYRMIYGFLMRKLYKNYEELKKMDY"
     assembly_gap    2775..2874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2875..3380)
                     /locus_tag="P5335_10065"
     CDS             complement(<2875..3380)
                     /locus_tag="P5335_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRZ75266.1"
                     /translation="MGLFKRNPFGHILLLKKWLIRFFGMITHQRYRGFNQMHIEGSEI
                     IKNLPDTGVLFISNHQTYFADVVAMFHVFNASLSGREDNIKNVGYMWHPKLNIYYVAA
                     SETMHEGILPRILAYAGAITVERTWRAKGKDIKREVNPNDTENIKIALNDGWVITFPQ
                     GTTRSFKPV"
     gene            complement(3433..4071)
                     /locus_tag="P5335_10070"
     CDS             complement(3433..4071)
                     /locus_tag="P5335_10070"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRZ75267.1"
                     /translation="MEFHNFLKYIPKIAKEKLPAANAHLKMAPIERIEMLKELNIDSK
                     NPRKAAVMMLFYPKNNQTHLLLIVRNTYPGVHSSQIAFPGGKEEEYDESLAHTALRET
                     EEEIGVQRHKIELIKSFTEVYIPPSNYLVSPFLGICQSELKFELQKDEVAGIIELPFN
                     DFLDDRKVVMRKLSTSYANEIDVPAFLVKEHIVWGATAMMMSELKETIKLVL"
     gene            4060..4764
                     /locus_tag="P5335_10075"
     CDS             4060..4764
                     /locus_tag="P5335_10075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRZ75268.1"
                     /translation="MKLHKILLLLLIFSVYSCEKKSKDTSEKNRVKENSHDSIKIEKP
                     KPKYDLETIVISDENLVDFLTWYGEQNNENKVLIETKFGNIEIELFYETPLHRANFIY
                     LVKKNYFNTTYFHRVVNNFIIQGGNSDESITKKERKNIGKYLIPAEFKKSLRHNYGAL
                     ASARSWDNNPEKLSNPYEFYFIQNKKGEHHLDGEHTVFGKITKGFDVLDEIASQKTDE
                     GEYPLLNIKIKAKIIN"
     gene            4764..5207
                     /locus_tag="P5335_10080"
     CDS             4764..5207
                     /locus_tag="P5335_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HRZ75269.1"
                     /translation="MFNKEEAQKIKREFWITFAEEYPRKWLLYNTRIKDFSFKFHVDN
                     KKAMVSLDIEPKDEEHRKIYFEKIESLKNILTEEYIYDAIFERNYQLDNGKIISRIWV
                     EIDNISINNKSTWPTVFEFFDKNMESYERFFYEYEDYIRDLEINT"
BASE COUNT         1894 a          870 c          870 g         1757 t
ORIGIN      
        1 cggtctaaac ggatgtaatt gtaaatccaa ttggtcaaca cgacggcttt atttttaaat
       61 ccgattaggg aaaataagtg tacaaaaatc caaacaaacc aagcaaaaac gccactaaaa
      121 tgaaacttgg gtaaatcaac cacggccttg tttcttccaa tggtagccat cgaacctttg
      181 tcgttgtaca caaaaggttt tagtggttgt ttattgattt ttagaacgat gttttcgcct
      241 aaccattttc cttgttgcat agcgggctga gccatcatgg gatgcccttt tggattttct
      301 tcggaaatca tggcggcaat atcacctacg gcaaaaatgt tatcgtaacc ttctacttgg
      361 ttaaattcat ttacttttac ccgatctgct cgtggtataa ttgcgtttac atcgattccg
      421 gacggcaatg ctccttgcac accggctgtc caaattactg tagcggtttc aaaggttaag
      481 tcggtattgg tggtaacggt gctgccatcg taaccggtaa cgcgaacgtt tttccagaca
      541 tttacgccta atttaatcag gaaatcttca gcagcatcag atgatttttc actcatggaa
      601 tccaaaattc tatcactgga ttggataagg ttgatttgca tttttcgcac atccaaatcg
      661 ggataatctt tggggagaat ggcttttttc atttcggcca aagctccggc caattctacc
      721 cctgtgggtc cgccacccac aagtacgaag ctgattaaac tatgtcggtc gtgaatatca
      781 tttgtaagca aggcttgttc aaaattttcg agaatcaaac ttctgatgtt gagcgattgc
      841 ggaatggttt tcattgccac actattgcgt tcgatttctt tgttgccaaa aaaattggtt
      901 ttggaacctg ttgccaatac caaataatca taccgcaatt cgccaatatc ggcttttact
      961 ttttggttct tggtatcaat ttcgttgaca aaacccaagc gaaaataaaa attttgatac
     1021 tcctgaacca cttttcgaat ggggtatgca atggaacccg cttcgagtcc gcccgtggcc
     1081 acttgatata aaagtggttg aaacgtatgg taattatgtt tgtcgagtaa aacaacttgt
     1141 actcgttggt tttttagttt tttggcgagg gcaattccgg caaaacctcc accgatgatg
     1201 acgattctgg gaagattagt atttgggatg ttcatttttt ttaaggttct aaggttctga
     1261 ggcactaagg ctctgaggtt ttgaatggta aagataagga gttttaacat ttgaaggatt
     1321 actccttttt tttttcttgt tgaaatttaa ctgaaattta taattttgat tttagttttt
     1381 ttgaacacag attaaaaaaa tgagataaat ttttgaaact tgaaacctga aacttttagc
     1441 cccgattgaa atggaaagcc tcttgaaaca aaatgtattt tttcttgctt gttcaaagcg
     1501 accaaaggaa gctcttgaaa aaaacatttt gagaaaagag cttgcaatga aaagcgggat
     1561 tagcttctga aaaaaatact atcttgtaac aaatttagca ctcaagctac taacacgaca
     1621 tgagcaattc actggagcaa tcttttgtca agcaattaaa ggaaaatcag aacattattc
     1681 acaagatttg taggttatac acatccggtg aagatgctca taatgattta ttccaggaaa
     1741 ttacaattca gttatggaaa gcgtttccga agtttcgagg cgaatcaaaa ttctctactt
     1801 gggcctatcg tgtagcgttg aataccgcca ttacgttgta tcgaaaaagc acacgaaccg
     1861 ttgccaccac cgacatggaa ggcaaaaaac aatttgtagc tcccgaagat tataattttg
     1921 aagaagaaga acaaattaaa ttgttataca aagccgtaca acaattgaat gatattgaaa
     1981 aagctttggt ttttatgtat ttggaagaca aagattatga agaaattgcc gaaacattag
     2041 gaatcagtga agtaaatgca cgagtgaaga tgaatagaat taaaggaaaa ttaaaaaaaa
     2101 tagtaaatcc gtaggaacat gacagaggaa ttagatttat taaaaaaagc ttggaaaaag
     2161 gatgcacatt cttacgaaca agttactgaa aatcaaattt atgcaatgat acataaacga
     2221 tcatcatcta ttgtcaaatg gattttgatg attagtattg ctgaattcat tatttggtca
     2281 ggtattggct tttttacagt ggatgataat tatttgaaga ctttagaaat atatcatatt
     2341 gacaaacttt ttaaaataat aaccggattt aattatgcaa ttgtaatagt tttcatctac
     2401 ctgttttata aaaactttaa aactatatca acgaatgaaa cagtcaaaaa attgatgagt
     2461 tcgattataa aaacccgtaa aacagttcag tattatattt ggtataattt gaccatgttt
     2521 gccattattt ttataattgt ggttatttca caaataacgt atgatccaaa cctgaactca
     2581 gttatcgaaa acacaaaaga aatgaatcct caaacatttt gggttattat tggactaatc
     2641 tattttgcct tatttgcagt gacctttggt ttattttggt tattttaccg aatgatatac
     2701 ggattcttga tgagaaaatt atataaaaac tatgaagaat taaaaaagat ggattattaa
     2761 tattcccact ttctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaccggt
     2881 ttaaatgaac gggtagttcc ttgcggaaag gtgattaccc aaccgtcatt caaggctatt
     2941 ttaatatttt cagtatcgtt cggattaact tctcgtttaa tatcttttcc tttagctcgc
     3001 caagttcgtt ctaccgtgat tgcaccggca taagctaaaa ttctaggcaa tatgccttca
     3061 tgcatagttt cactagccgc tacataatag atatttaatt tgggatgcca catataaccc
     3121 acatttttga tgttgtcctc tctcccactc aaactggcat taaacacatg aaacatagca
     3181 acaacatctg caaaatacgt ttgatggtta gaaataaata aaacaccagt atcaggtaag
     3241 tttttgataa tttcagaacc ttcaatatgc atttgattaa accctctgta acgttgatgt
     3301 gtaatcattc cgaaaaaacg gatgagccac tttttaagta atagaatatg tccgaaagga
     3361 tttcttttaa acaatcccat aataatttta ttggagaata ataaggtggc taatttataa
     3421 aaaacaaact aattaaagta ccaattttat ggtttctttc agttcactca tcatcatagc
     3481 agtggcaccc caaacaatgt gctcttttac taaaaaagct ggaacatcaa tttcattagc
     3541 ataagatgtt gatagctttc tcatgaccac ttttctgtca tctaaaaaat cattgaatgg
     3601 aagttcaata ataccggcaa cttcatcctt ttgcaattca aatttcaatt cagattgaca
     3661 aatccctaaa aaaggagaaa caagataatt acttggagga atataaactt ctgtaaacga
     3721 ttttatcaat tcaattttat gtctttgcac tcctatttct tcctctgttt cacgcaaagc
     3781 agtatgagct aatgattcat catactcttc ttctttacct cctggaaaag caatttgaga
     3841 agaatgaaca cctggataag tatttctaac gattaataaa agatgtgtct gattattttt
     3901 tggataaaaa agcatcatta cagctgcctt acgtggattt ttactatcaa tattcaattc
     3961 tttaagcatt tcaattcttt cgataggtgc catttttaaa tgagcatttg cagcaggaag
     4021 cttttctttt gctatttttg gaatatattt taaaaaatta tgaaactcca taaaattctg
     4081 cttttattac ttattttttc tgtttattct tgtgaaaaga aatctaaaga tacatcagaa
     4141 aaaaatagag tcaaagaaaa tagtcatgat agtattaaaa ttgaaaaacc aaaaccaaaa
     4201 tatgatttgg aaacaattgt aatatcagat gagaatttag ttgattttct tacttggtat
     4261 ggagaacaaa acaatgaaaa taaggtttta attgaaacta aatttggaaa tattgaaatt
     4321 gaattatttt atgaaacacc acttcataga gcaaatttta tttacttagt aaaaaagaac
     4381 tatttcaata ctacttattt ccatagggtg gttaataatt ttataattca aggtgggaat
     4441 tcggatgaaa gtatcacaaa aaaggaacga aaaaatattg gaaaatattt aattccggct
     4501 gaatttaaaa aatcacttcg tcacaattac ggagctttag catcagctag aagttgggat
     4561 aacaatcctg aaaaattaag caatccttat gaattttatt ttattcaaaa taaaaaagga
     4621 gaacatcatt tagatggtga acataccgtt tttggaaaaa tcacaaaggg ttttgatgtt
     4681 ctagatgaaa ttgcttccca aaaaacagat gaaggggaat atcctcttct aaatattaaa
     4741 ataaaagcca aaattatcaa ctaatgttca acaaagaaga agcacaaaag ataaaacgtg
     4801 aattttggat aactttcgca gaggaatacc caagaaaatg gcttttatac aatactagaa
     4861 taaaagattt ttcatttaaa tttcacgtcg acaacaaaaa agcaatggtt tctttagaca
     4921 tcgaacccaa agatgaagaa caccgaaaaa tctatttcga aaaaatagaa tcattaaaaa
     4981 atattttaac tgaagaatat atttacgatg ccatcttcga aagaaactac caactagata
     5041 acggaaaaat catcagcaga atttgggttg aaatcgacaa catcagcatc aacaacaaat
     5101 ctacatggcc tacagttttt gaattttttg ataaaaacat ggaatcttat gaacgtttct
     5161 tttatgaata tgaagattat attagagact tagaaatcaa tacatgacat ttatttaatc
     5221 aattataaac ctatcttttt attgttaata atttaaattg attcgtctta aggaattcaa
     5281 aaaaatatat ctagtatatt aattgtataa atttatattg acaataattt taaaaaagaa
     5341 attgaacata ttctttacac tatttcgaag ccaacaaacg aaataaattg cagtactatc
     5401 ttctccattg tcctaaaact tcagacttaa cttctaaatt tccatattca atatcattta
     5461 ttctttttca aaaacaaaaa atcctcatca a
//
LOCUS       DAOTCW010000148         5420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_11321_length_5420_cov_27.021994, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000148 DAOTCW010000000
VERSION     DAOTCW010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11321_length_5420_cov_27.021994"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="P5335_10085"
     CDS             complement(<1..527)
                     /locus_tag="P5335_10085"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HRZ75270.1"
                     /translation="MKPLVVIPARGGSKGIPGKNIKLLGGIPLIQYTLEAAKALFPKE
                     IICVSTDDLEIKKCVEKLGFKVPFLRPSELATDSSGTYEVLLHAIEFYENNGYYPDTL
                     ILLQPTSPFRNSKHIEQAINLYTSEIDMVVSVKETKANPYYNLFEEDSGGFLKKSKEA
                     TFNRRQDCPAVWQYNG"
     gene            complement(529..1575)
                     /locus_tag="P5335_10090"
     CDS             complement(529..1575)
                     /locus_tag="P5335_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRZ75271.1"
                     /translation="MKNHREHLILKGSSIREALIQLDLLAKDAIIFVVDKEDKLLGSI
                     TDGDIRRGLINGAAIDDKIETVIHPNPKFLCRGEYDVEKMISLREGNFRIIPILDKEA
                     KVVNVINFREIRSYLPIDAVIMAGGKGERLKPLTDKIPKPLLKIGSKPILEHNIDRLI
                     LYGIDDFWISVNYLGEQIKEHFNPGYKKNIVINFVQEEVPMGTIGAVSNITNFEHDYV
                     LIINSDVLTNIDYEHFYLDFIKQDADFAVVSIPYEVSVPYAVLETSNGHILSLKEKPT
                     YTYYSNGGIYLMKRFVLQMLPKNVFFNATDFMEKLIHDNYKVVSYPLLGYWLDIGKHE
                     DFEKAQRDINQIKF"
     gene            complement(1572..2675)
                     /gene="neuC"
                     /locus_tag="P5335_10095"
     CDS             complement(1572..2675)
                     /gene="neuC"
                     /locus_tag="P5335_10095"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HRZ75272.1"
                     /translation="MKIGILTSSRADYGIYQPLLKKMSNDSYFQVEIIAFGTHLSKLH
                     DYTLKIIEKDNFGKVYALETLIDDDTQLGTAKSYAKTISVFADFWNENNYDLVLCLGD
                     RFEMNAAVQSGIPFGVKFAHLYGGETTLGAFDNIFRHQISLASQLHFTATEQFSKRVS
                     EITQDFSNVFVIGSLSLDELFSFQPKPKTIFLEQFGISKGDYVLVTFHPETMSFDKNT
                     IYAKEMRLALSKISEHMNVVVTMPNADTRGSVIRQELILLHSEHLDRVVLVENFGKDN
                     YFTAMYHSKMLIGNTSSGIIEAASFNKYVINVGNRQKGRLQSKNIVNCDFIASDIYNV
                     AMKLYTSKPYEGENIYFRKGAADKVIKILKEFG"
     gene            complement(2672..3691)
                     /gene="neuB"
                     /locus_tag="P5335_10100"
     CDS             complement(2672..3691)
                     /gene="neuB"
                     /locus_tag="P5335_10100"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009416925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HRZ75273.1"
                     /translation="MIKTIIIAEAGVNHNGNIAMAKQLIDVAADAKVDYVKFQTFKAD
                     TIVSPLAKKATYQIQDTNEDDLQYTMLKKLELSEQDHLELIAYSTKRGISFFSTAFDV
                     EGINFLDNIGLDIFKIPSGELTNFPYLKAIAHKGKPVIMSTGMASLEEIKNALAVLER
                     FGVERSSITILHCNTEYPTPMEDVNLKAMLTIGNHFGVNVGYSDHTLGIEVPIAAVAL
                     GAKVIEKHFTLDRNLPGPDHKASLEPLELKKMVEAIRNVEKAICGDGEKKPSKSEFKN
                     ILIARKSIHLRGDLMQGHILTEADIIALRPGDGISAMEWENVIGKKLITDKNAFDKLN
                     WTDLQ"
     gene            complement(3688..4326)
                     /locus_tag="P5335_10105"
     CDS             complement(3688..4326)
                     /locus_tag="P5335_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HRZ75274.1"
                     /translation="MLDFTKKIAIFGIGGLAKDVFFCLKEIYLKGHYAFDGQVVFVDK
                     LPNKSAFFLNCPLFLEENFDSRAYQVIIAIGNPKIRKQISISLPAATFYPKVIHPSAL
                     VNETVILGKGVVILANAIISCDVTVGDFCILDRAVQIGHDCMVADFVHFSPASVLSGN
                     VTINSLVDIGTLAAIKQNLEVERDTIIGMGTIVVKSIQEKGVYVGNPAKKIR"
     gene            complement(4328..5329)
                     /locus_tag="P5335_10110"
     CDS             complement(4328..5329)
                     /locus_tag="P5335_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HRZ75275.1"
                     /translation="MNTLQNKKILVTGADGFIGSHLVDYLVSQGYQVKAFCFYNSFNS
                     WGWLDSFDKEVLQKIEVFTGDIRDPNGVREAMKDCQVVFHLAALIAIPFSYHSPDSYI
                     DTNVKGTLNILQAARDLKVERVLVTSTSEVYGTAQYVPIDEKHPKQPQSPYSASKIGA
                     DAIADSFYRSFNLPVTIVRPFNTFGPRQSARAFIPTIITQLLNGKTDIKLGDVTPTRD
                     LLFVKDTVRAFEAIANCDELIGHEVNIATNSEISIQEVADLLIQQVNPKAKIITDGDR
                     IRPEKSEVFRLFGDNTKLRNYTNWTVETDFNQAILETIAWFKVKENLAHYKADIYNL"
BASE COUNT         1741 a         1044 c          721 g         1914 t
ORIGIN      
        1 ccattatatt gccaaacagc aggacaatcc tgtcttctgt taaatgtggc ttcctttgat
       61 ttttttaaaa aacctcctga atcctcttca aaaagattat aatatggatt ggcttttgtt
      121 tctttaacag aaaccaccat atcaatttcg gaagtatata aattgattgc ttgttctata
      181 tgttttgaat tcctgaaagg tgaagtgggc tgcaataata ttaaagtatc cggataatac
      241 ccattatttt cataaaattc aatagcatga agtaatacct cataagttcc acttgaatct
      301 gtagccaatt cagatggcct tagaaacggt actttgaaac ctaatttttc tacacatttt
      361 tttatctcta aatcatcagt agacacacaa ataatctctt ttggaaataa tgctttagca
      421 gcttcaaggg tatactgaat gagtgggatt cctcctagta actttatgtt tttacctgga
      481 attccttttg atccacctct ggctggtatg actactaatg gtttcaaatt aaaattttat
      541 ttgatttata tcacgttgtg ctttttcaaa atcttcatgc ttcccaatat ctaaccaata
      601 acctaacaaa ggatacgaaa ccactttata attatcatgg attagctttt ccataaaatc
      661 agtagcatta aaaaatacgt ttttgggaag catctgtaaa acgaatctct tcattaaata
      721 aatacctccg tttgaatagt aggtataggt tggcttctct tttaaactta atatatgtcc
      781 gttactagtt tctaataccg cataaggcac actcacttca taaggaatag aaactactgc
      841 aaaatcggca tcttgtttga tgaaatctaa ataaaagtgt tcataatcta tattagttaa
      901 tacatcagaa ttgattatca aaacataatc atgttcaaaa tttgtaatgt tactaactgc
      961 accaatggtt cccataggca cttcttcctg aacgaagttt attacaatat tttttttgta
     1021 tcctgggtta aaatgctctt ttatctgttc tccaagataa tttactgaaa tccaaaaatc
     1081 atcaatacca tataaaatca atcgatcaat attgtgttct aaaatgggtt tacttccaat
     1141 ttttaataat ggttttggaa ttttatccgt caaaggttta agacgttctc ccttacctcc
     1201 tgccattatt actgcatcaa taggaagata tgatcgtatt tccctgaaat taatcacatt
     1261 caccaccttt gcttctttat ctaaaattgg aataattcta aaattgcctt cccttaatga
     1321 tatcattttt tccacatcat actcacctct gcataaaaac tttggattag ggtgaatcac
     1381 agtttctatt ttatcgtcaa tcgctgcacc atttattaat cctctcctaa tgtcaccgtc
     1441 agtaatagaa cctaataact tatcctcttt atcaactaca aaaattatgg catcttttgc
     1501 caataaatct aattgaatta acgcttctct tatagaactt cctttaagta ttaaatgttc
     1561 tctgtgattt ttcatccaaa ttcttttaaa atttttatta ctttatcagc tgcacctttt
     1621 ctaaaatata tattttctcc ctcgtatggc ttcgatgtat ataatttcat tgctacatta
     1681 tatatatcac ttgcaataaa atcacaattt actatatttt tgctttgcaa tcttcctttt
     1741 tgcctgttac caacattaat aacatactta ttaaaggaag ctgcttcaat aatcccactg
     1801 gatgtatttc ctatcaacat tttagaatga tacattgctg taaaataatt atctttccca
     1861 aaattctcaa ccaatacaac tctatctaaa tgctctgaat gtaacaaaat gagttcttgt
     1921 cttattacgg aaccccttgt atcggcattc ggcatagtaa caacaacatt catatgttct
     1981 gaaattttac ttaaagctag tcgcatttct tttgcataaa tagtgttttt atcaaaagac
     2041 attgtttccg ggtgaaaagt aactaataca taatctccct tagaaatccc aaattgctct
     2101 agaaaaatag tctttggttt tggttgaaat gaaaataact catctaaact caaagagccg
     2161 ataacgaaaa catttgaaaa atcttgagta atctctgaaa ctcttttact gaattgctcc
     2221 gtcgctgtaa aatgtaattg ggatgccaaa gaaatttgat gcctaaaaat attatcaaat
     2281 gctcctaaag ttgtttcacc accatataaa tgtgcaaatt tgacaccaaa tggtataccg
     2341 gactgaacag ccgcattcat ttcaaatctg tctcctaaac acaaaaccaa atcgtagttg
     2401 ttctcattcc aaaaatcagc aaaaactgag attgttttag catatgattt tgcagttcct
     2461 aactgtgtat catcatcaat caatgtttct aatgcataaa cttttccgaa attatctttt
     2521 tcaataatct taagtgtata atcatgcaat tttgacagat gcgttccaaa agcaattatt
     2581 tccacttgaa aataggaatc gttactcatt tttttaagca acggttgata aatgccgtaa
     2641 tcggctcttg aacttgttaa aatgcctatt ttcattgtaa atcagtccaa tttaatttat
     2701 caaaagcatt tttatctgtt attaactttt tacctattac attttcccat tccatagctg
     2761 aaattccgtc accgggtctt aaagcaataa tatctgcctc tgttaaaata tgtccttgca
     2821 ttaaatcacc tcttaagtga atgctttttc ttgcaattag tatgttttta aattcgcttt
     2881 tacttggctt tttttcacca tctccacaaa tagctttttc tacatttcga atcgcttcta
     2941 ccattttctt taattctaag ggttccagtg atgctttatg atccggacca ggtaaatttc
     3001 tgtctaatgt aaaatgtttt tctatcactt tagctcccaa agctaccgca gcaataggga
     3061 cttcaatgcc taatgtatga tcagaatatc cgacattcac accaaaatga tttccaatag
     3121 tcaacatggc ttttagattt acatcttcca ttggtgtagg atattctgta ttacaatgta
     3181 aaattgtaat actagatcgt tctactccaa aacgctctaa aacagctaaa gcatttttaa
     3241 tttcttccaa acttgccatt ccggttgaca ttataaccgg cttcccctta tgtgcaattg
     3301 ctttcaaata cggaaagttt gttaattccc cacttggaat cttaaaaata tcaagaccaa
     3361 tattgtctaa aaaattaatt ccttcaacat caaatgcagt agaaaaaaaa gaaatccctc
     3421 tttttgtaga atacgctatt aattctaaat gatcctgttc agataactct aattttttaa
     3481 gcattgtata ctgtaaatca tcttcattcg tatcttgaat ttgataagtt gcttttttgg
     3541 ctaaaggact tacaatagta tctgctttaa acgtttgaaa tttaacataa tctacttttg
     3601 catcagccgc aacgtcgatt aattgcttag ccatcgcaat atttccatta tgatttactc
     3661 ctgcttctgc aataataatc gtttttatca tcttatcttt ttggccggat ttcctacgta
     3721 aactcctttt tcttgaattg acttaacaac aatagttccc atgccgatga tggtatctct
     3781 ctccacttct aaattctgtt taatcgctgc aagtgtccct atatcaacta aactattaat
     3841 tgtaacgttt ccactgagca cagaagccgg agaaaaatgc acaaaatcgg caaccataca
     3901 atcatgtcca atctgtacgg cacgatccaa tatgcaaaaa tcacccaccg ttacatcgca
     3961 acttataatt gcatttgcta aaattacaac tccttttcct aaaataaccg tttcattaac
     4021 caaagcactt ggatgaataa cctttggata aaaagtagct gccggcaaac tgattgaaat
     4081 ttgttttctg attttaggat taccaatagc aataataacc tgataggctc gtgaatcaaa
     4141 attttcttcc agaaataagg gacagtttaa aaaaaaagca cttttattgg gcaacttatc
     4201 cacaaaaaca acttgaccat caaaagcata gtggcctttt aaataaatct cctttaaaca
     4261 aaaaaatacg tcttttgcca atccaccaat accaaaaatg gcaatttttt ttgtaaaatc
     4321 taacatgtta tagattataa atatccgctt tataatgggc taaattttct ttcactttaa
     4381 accatgctat agtttctaaa atagcctgat taaaatcagt ctccacagtc caatttgtat
     4441 aatttcttaa tttggtatta tctccaaaca aacgaaacac ttcgcttttt tcaggtctga
     4501 ttctatctcc atccgtaata atttttgctt ttggatttac ttgttgtatt agtaaatcgg
     4561 caacttcctg aatgcttatt tctgaatttg tagcaatatt tacttcatga ccgattaatt
     4621 catcacaatt tgctattgct tcaaaagctc gaacggtatc tttcacaaac aacaaatctc
     4681 tggtaggagt tacatcacct aatttaatgt ctgtcttacc attcaaaagt tgtgtaataa
     4741 tggttggaat aaatgctctg gctgattgac gtggaccaaa tgtattaaat ggtcttacaa
     4801 tagtgaccgg aagattaaaa cttctgtaaa aggaatctgc aattgcatct gcaccaattt
     4861 tagaggctga atagggtgat tgtggctgtt tcggatgctt ttcgtcaatc ggaacatact
     4921 gagccgttcc atatacttct gaagtagaag taaccaaaac ccgttcaact tttaaatctc
     4981 gagcagcttg taaaatattc aatgttccct ttacattggt atcaatatag gaatcgggag
     5041 aatgataact gaaaggaata gcaataagtg ccgccaggtg aaagactact tgacaatctt
     5101 tcattgcttc ccgtacgcca ttagggtcac gaatatcacc cgtaaatacc tctatttttt
     5161 gcaaaacttc tttgtcaaaa ctatcgagcc aaccccatga attaaaagag ttgtaaaaac
     5221 aaaaggcttt cacttgatag ccttgtgata ctaaatagtc aactaaatgg gaaccaataa
     5281 aaccatcggc tcccgtaact agtatttttt tattttgtaa agtattcatt ctcaaattat
     5341 acttttaatt agtttaattg aattgttgcc tcttgaataa atccttttgg tctgtttaaa
     5401 acgccggaat gccaataatc
//
LOCUS       DAOTCW010000149         5401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_11397_length_5401_cov_42.554433, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000149 DAOTCW010000000
VERSION     DAOTCW010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11397_length_5401_cov_42.554433"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="P5335_10115"
     CDS             <1..882
                     /locus_tag="P5335_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRZ75276.1"
                     /translation="EYFKNGTPEEQALALKADVLWKGVEWSWYTKGENAMYWHWSPNY
                     EWQKNFKLEGYNECLITYIMGASSPTHPIPNEAYHQAWARNGTIVHPGLKYNIPVVFN
                     YNGATGNVGPMFWAHYSYLGLDPRGLSDQYANYWELTQNHAKIMHAYCVANPVGWTGY
                     SDECWGLTASYSRNNNGSVGYTDHKPGNDRGVISPTAALSSFPYTPVESMKALRYFYE
                     SADWKDKLIGVAGPYDAFSPHHNWVTKRYLAIDQGTIVPMIENYRTGFLWNLFMQAPE
                     VKTGLQNLGFSSTQHGF"
     gene            943..>1982
                     /locus_tag="P5335_10120"
     CDS             943..>1982
                     /locus_tag="P5335_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRZ75277.1"
                     /translation="MKTTLLLLFISFLGTAQKVDKTKNTIPKAQFIEELIAKMTVEEK
                     LGQLNLPTSGDITTGQANSSDVAKNIKEGKVGGLFNIKSASKIREVQKIAVEQTRLKI
                     PLLFGMDVIHGYETTFPIPLGLSSTWNLSLIERSAQIAAKEASADGINWTFSPMVDIS
                     RDPRWGRVSEGSGEDPYLGSQIAKAMVRGYQGNDLSLNSTILACVKHFALYGAPEAGR
                     EYNTVDMSRIRMYNDYFPPYKAAVDAGVGSVMASFNEVDGVPATANKWLMTEVLRNQW
                     KFKGFVVTDYTAVQELMEHGIGDFQTVSAAALKAGIEMDMVSGGFLNTLKKSLDEGKV
                     SMQQIDNAVRLI"
     assembly_gap    1983..2082
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2083..3072
                     /locus_tag="P5335_10125"
     CDS             <2083..3072
                     /locus_tag="P5335_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRZ75278.1"
                     /translation="VSVFKGMQEILGQSAKVIYAKGSNLTEDAAYEERATMFGKTLNR
                     DNRTKEQLLNEALQIANQADVIVATLGESSEMSGESSSRTELEIPSVQKELLTALLKT
                     GKPVVLVLFTGRPLVLVEEHKNLSAILNVWFPGTEAGYAITDVLFGDENPSGKLTATF
                     PRSVGQIPIYYSHKNTGRPLTNKEGKFVKFLSNYIDERNEPLYPFGYGLSYTTFKYDN
                     LKVSSPKMTFSDKITVSIDITNTGNFDGKEVVQLYSRDVVGSVTRPMKELKGFKKVFL
                     KKGEKQTVSFELSVDDLKFYNSDLEFVAEPGVFELFIGANSDVQQKVSIELVK"
     gene            3141..4895
                     /locus_tag="P5335_10130"
     CDS             3141..4895
                     /locus_tag="P5335_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HRZ75279.1"
                     /translation="MRKITLLMLFLLGIQTYAQQKTYCNPINIDYGYCPIEPFVTQGK
                     HRATADPVITFFKGKYYLFSTNQWGYWHSEDMLNWKFVSRKFLRPEHNVWDELCAPSL
                     SFVNDTLLVIGSTHTKEFPIWMSKNPEVDDWKELVHKQQAGAWDPQIFWDKEKDDLYL
                     YYGSSNLYPLYGLKLNRKTFQPEGEPVPVLALNDHEHGWERFGENNDNTFLQPFTEGA
                     FMTKYNNKYYLQYGAPGTEFSGYADGVYVGDGPLGPFEYQSHNPFSYKPGGFARGAGH
                     GATFQDNKEQYWHVSTIGICVKNNFERRLGIWPAGFDKDGIMYSNTAYGDYPTYLPSQ
                     NKNHLNENFAGWMLLNYNKPVHVSSTLGGYQPNFAVNEDIKTYWSAKTGNKGEFIISD
                     LGEMSTVNAIQINFADQDADIMGKPETTTGHNYILYASDDGKKWRIIADKSKSRNDVP
                     HEYIEFQTPFKARFIKLENYQMPTGKFALSGFRVFGNGSGEKPQAVKNFVPLRSGPKV
                     EGERRNVWFKWQQEPSADGYVIYFGKSPNKLYGSIMVYGKTEYYFNGLDRSDIYYFQI
                     EAFNNNGIGPKSEIKKSE"
     gene            5139..>5401
                     /locus_tag="P5335_10135"
     CDS             5139..>5401
                     /locus_tag="P5335_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRZ75280.1"
                     /translation="MSAEQVQTKWGQFIPLVTVFFFWGFVAASNDILIPVFKKAFDLT
                     QGESQLVSVAFYVAYTVGSIIYMIISYLTKGDLINRIGYKNSLA"
BASE COUNT         1775 a          933 c         1050 g         1543 t
ORIGIN      
        1 gaatatttta aaaatggaac tcctgaagaa caagcactgg ctttaaaagc cgatgtattg
       61 tggaaaggag tagaatggag ctggtatacc aaaggcgaaa acgccatgta ttggcattgg
      121 tctccgaact acgaatggca aaagaatttt aaattagaag gatataacga atgtttaatt
      181 acgtatatca tgggagcatc ttctcccaca catcctattc ctaatgaagc ttatcatcag
      241 gcatgggcaa gaaatggaac cattgttcac ccgggattaa aatataatat tccggttgtt
      301 tttaattata atggagcaac aggaaatgtt ggtccaatgt tttgggcaca ttattcttat
      361 ttgggtttag acccaagggg attaagtgac caatatgcta attattggga gctaactcaa
      421 aatcatgcta aaatcatgca tgcttattgt gttgcaaatc cagtgggttg gacaggttat
      481 agcgacgaat gttggggatt aacagccagt tattctagaa acaataacgg ttcggttggc
      541 tacactgacc acaaaccggg caacgataga ggagtaattt ctcctacagc tgcgttatct
      601 tctttcccgt acactcctgt agaatcaatg aaagcccttc gttactttta tgaaagtgcg
      661 gattggaaag acaaattgat tggagttgcc ggaccttatg atgctttttc accacaccat
      721 aattgggtga ctaaacgata tcttgcaatt gatcaaggaa caatcgttcc aatgattgaa
      781 aattacagaa cagggttctt gtggaattta ttcatgcaag ctcctgaagt gaaaaccgga
      841 ttacaaaatt taggtttttc atctacccaa catggattct aaaagataaa aggaatattt
      901 ttaaaagata atccaattac aaaaaacata aaaatgaatt taattaaaac caccctatta
      961 cttttattta tttctttttt gggtactgct cagaaggtag ataaaacaaa aaatacaatt
     1021 ccaaaagctc aatttattga agagttaata gctaaaatga cagtggagga aaaactagga
     1081 caacttaact tgcccacatc cggggatata acaaccggcc aggcaaatag ttcagatgtc
     1141 gcaaaaaata ttaaagaggg taaagtaggg gggttattca atataaaatc agcttctaaa
     1201 attagagagg ttcaaaaaat tgctgtagaa caaacgcgtt taaaaatccc acttcttttc
     1261 ggaatggatg ttattcacgg gtatgaaact acttttccaa ttcctttggg attatccagc
     1321 acatggaact taagtttaat tgaacgcagt gctcaaattg cagccaaaga agccagtgct
     1381 gatggtatca attggacttt ttcacccatg gttgatattt cacgtgaccc acgctggggt
     1441 agagtttcgg aaggttccgg tgaagaccct tacctcggaa gtcaaattgc aaaagcgatg
     1501 gtaagagggt atcaaggaaa cgacttgtca ctcaattcaa ccattttagc gtgtgtcaaa
     1561 cattttgctt tatatggtgc tcctgaagcg ggtagagaat acaacacagt cgacatgagt
     1621 cgcattcgta tgtacaatga ttattttcca ccttacaaag cagccgttga tgccggagtt
     1681 ggttcagtga tggcttcttt taatgaagtg gatggtgttc ctgctactgc taataaatgg
     1741 ttaatgacgg aagttttacg aaaccaatgg aaattcaaag gatttgtagt aacagattac
     1801 actgctgttc aagaattaat ggaacacgga attggcgatt ttcaaacagt ttccgctgcg
     1861 gctttaaagg caggaattga aatggatatg gttagcggtg gttttttaaa cacacttaaa
     1921 aaatcgttag acgaaggcaa agtgtccatg caacaaattg ataatgccgt tcgattaatt
     1981 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtttcagt tttcaaagga
     2101 atgcaggaaa tactcggaca atcggcaaag gtaatctatg ccaaaggaag caatttaacc
     2161 gaagatgcgg cctatgaaga acgggcaacc atgtttggaa aaacattaaa cagagacaac
     2221 cgaacgaaag aacaactctt aaacgaagcg ttgcaaattg caaatcaagc ggatgtaatt
     2281 gtagctacac tgggagaatc ttccgaaatg agtggagaat ccagtagccg tacggaatta
     2341 gaaattccaa gtgttcaaaa agaattatta acggccttat taaaaacagg aaaacccgtt
     2401 gttttggtat tatttactgg ccgtccgttg gttttagtag aagaacataa aaacctttct
     2461 gccatcttaa acgtttggtt tccaggaaca gaagcaggct atgccatcac cgatgtttta
     2521 tttggcgacg aaaacccttc cggaaaattg acagcaactt tccctagaag tgttggacaa
     2581 ataccaattt attacagcca taaaaataca ggaagaccct taacgaacaa agaaggtaag
     2641 tttgtaaaat ttctttcaaa ttatattgac gaaagaaatg aaccacttta tccttttgga
     2701 tatggattaa gttataccac ttttaaatac gataatttaa aagtgtcgag cccaaaaatg
     2761 actttctctg ataaaataac agtttcaata gacataacta acacgggtaa tttcgacgga
     2821 aaagaagtcg ttcaattata tagcagagat gtcgtgggtt cagtgacaag accaatgaaa
     2881 gagttaaaag ggtttaaaaa agtattcctt aaaaaggggg aaaaacaaac cgtttcattt
     2941 gaactgtctg tagacgattt aaaattttat aattccgatt tagaatttgt tgctgaaccg
     3001 ggagttttcg aattatttat cggagccaat tccgatgtac aacaaaaagt tagcattgag
     3061 ttagttaaat aattttttga gtttttgttc gaggaaaccc tttggattcg ttcgaagggt
     3121 ttttttaaat aacttgtagt atgagaaaaa tcactttatt gatgttgttt ttattgggaa
     3181 tacaaacgta tgctcaacaa aaaacatatt gtaatcctat caatatcgat tacggctatt
     3241 gtcccattga accatttgta acacaaggta aacatcgggc tacagccgat ccagtcatta
     3301 cgtttttcaa agggaaatac tatttgtttt ccaccaacca atggggatat tggcacagcg
     3361 aagatatgct caattggaaa tttgtttccc gaaaatttct tcgtcccgaa cacaacgttt
     3421 gggatgagtt atgtgctcct tctttgtctt ttgtaaacga tacactttta gtaattggtt
     3481 ccacccatac taaagaattt cccatttgga tgagtaaaaa tcccgaagta gacgattgga
     3541 aagaacttgt tcacaagcaa caagcaggag cttgggaccc acaaatcttt tgggataaag
     3601 aaaaagatga cctctatctg tattatggtt caagcaattt atatccttta tacggattaa
     3661 aactaaaccg aaaaacgttt cagccggaag gagaaccagt tcctgtttta gccttaaacg
     3721 accacgaaca cggttgggaa cgatttggtg aaaacaacga caatacattt ctccaacctt
     3781 ttacggaagg tgctttcatg actaaataca ataacaaata ttacctacaa tatggagcac
     3841 ccggaacaga gtttagtggc tatgccgatg gggtttatgt gggtgatggt ccgctaggcc
     3901 cttttgaata ccaatcacat aacccgtttt cctataaacc cggcggtttt gccagaggag
     3961 cagggcatgg ggcaactttt caagataaca aagaacaata ttggcacgtt tccaccatcg
     4021 gaatttgtgt taaaaacaat tttgaaagaa gattaggtat ttggcctgcc ggatttgata
     4081 aagacggaat aatgtacagc aatacagcct acggtgatta tccaacctac ttgccttctc
     4141 aaaacaaaaa tcatctaaat gagaattttg ccggttggat gttgttgaat tacaataaac
     4201 ccgtgcatgt ttcctctacc ttaggtggat accaaccaaa ctttgccgtt aacgaagata
     4261 tcaaaaccta ttggagtgca aaaacgggca acaaaggcga attcattatt tctgatttag
     4321 gtgaaatgag caccgtgaat gccatccaaa tcaattttgc cgaccaagat gctgacatca
     4381 tgggaaaacc cgaaacgaca acgggacaca actatatttt atacgcttct gacgatggta
     4441 aaaagtggag aatcattgcc gacaaaagca aaagtagaaa tgatgtcccg catgaatata
     4501 tcgaatttca aacacccttt aaagctcgat ttattaagct tgaaaattac caaatgccta
     4561 ccggtaagtt cgccctaagc ggatttcgtg tgtttggaaa tgggtccggt gaaaaaccac
     4621 aagcggtgaa aaactttgtc cccctccgtt ccggacccaa agtggaaggc gaacgcagaa
     4681 atgtttggtt caaatggcaa caggaacctt ctgcagatgg ctatgtcatt tatttcggca
     4741 aatcacccaa taaattatac ggttccatca tggtttatgg aaaaacggag tactatttta
     4801 atggtttaga tcgttcggat atttattatt ttcaaatcga agcgtttaat aacaacggaa
     4861 ttggtccgaa atcagaaatt aaaaaaagcg aataaaccga atttaggctt ttcgcaaacg
     4921 atgtattttt tttctttttt caaaaaaatg tagctttgaa aacgatttcg ttgaaaacta
     4981 tttataaata attggcttta cattttgaca taaatgtata tttaggaatt atttccgcaa
     5041 aaaattagca gtcttttagt aaagacacat tttttttcag gatttaataa aatttaattt
     5101 caaaacaagt tcaaaactaa tctcttaacc aaaaccaaat gagtgcagaa caagtacaaa
     5161 ccaagtgggg gcaattcatt cctctagtaa ccgttttttt cttctgggga tttgttgccg
     5221 ccagcaacga tattttgatt cccgttttca aaaaagcatt tgatttaacc caaggagaaa
     5281 gccaattggt ttcggttgct ttctatgtag cctatacggt gggctccatt atttacatga
     5341 ttatttctta tttgaccaaa ggtgatttga ttaatagaat tggctataaa aattccttgg
     5401 c
//
LOCUS       DAOTCW010000150         5363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_11513_length_5363_cov_48.439525, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000150 DAOTCW010000000
VERSION     DAOTCW010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11513_length_5363_cov_48.439525"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5363)
                     /locus_tag="P5335_10140"
     CDS             complement(<1..>5363)
                     /locus_tag="P5335_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRZ75281.1"
                     /translation="DFSLFKDYVLNFSSGLVSSNTDIRVTLAFDKAEWQPNQEIDQSL
                     FDISPAVKGKMVALASNTIAFIPEKPLEQDTEYRIIFKLSKLIKTPKNLSEFKFSIKT
                     LKQDFIVNVLDLQSYNREWQFLNGTIQTSDQMSFETASKLVEASQNGTKLKIKFDKEL
                     SRGTEFKFIIDSIKREVEDSKIKISWNGNPFDINQKGMVDFEIPGKNNFKIVGLEVGD
                     DDNQSLLINFSDPIKKGQDLRGLVSVETAIANKFSIEGNILKVFFSEPLKGDLLVEIF
                     QGIESEEGYKMKETHAQKVAFNEIKPGIRFMKNGTILPSSNNLKINFEAANLKAVDVK
                     IYKIYKNNILQFLQDNDLNGKRNLRKVATPVAKQTINLIDKKLPNYSKWNSFALDLAK
                     VITPEPGAIYRVELSFKKSYSLYKCSNSTTETESEEEESEEVRSNDDDYYDYYWYDDY
                     DWYDSKDPCENDYYSRGPIGMNVLASDLGVIAKRGENGSYLFAVTNIVNTQPVSGATV
                     ELYNFQQQKLASGITNVEGITKFELNKYAYFAIVTKGKQSTYVKLDEGYSLSVSNFDV
                     SGEKLQKGLKGYIYGERGVWRPGDTLFLSFMLNDAASKLDKSHPISFKLNDPNGKLTF
                     QSVQKYNDLNHYKFIIPTRDSHPTGNWEAVISVGGAKFYKSIKIETIKPNRLKIKNKF
                     DSEILSTSKINTSQVEVTWLHGAIAKDLKMEMQAKIMQQKTTFKGFANYNFDDPIRQF
                     ETEETTVFTGKVNSEGKATVNFQPKVSSQSPGMLKASLMTKVYENGGDFSADVMVATL
                     SPYKTYVGLKTPDPNKYGLLETGKMNRYDIVTVDEKGNPKAVQNLIVQVYKVEWRWWW
                     DASYDNLSSYVSNNSTSSYKNFAVSTNASGKASVQFKNEENEWGRYLIRVTDETGGHA
                     TGSTVMIDWPYWSGKTRNTDASNASMLMFSTDKKNYAVGEKAKISFPSSAGGRALISL
                     ENGSRVVKTFWATTQKGETKVEIPITGDMAPNVFVHITLLQPHASTLNDSPIRLYGIV
                     PIEVIDKNTIIEPQISMPKILKPEEKVNIRVSEKSGKAMTYTLAIVDDGLLDLTRFKT
                     PNAWNSFYTRDALGVKTWDIFDEIIGAYGGRINQVFSIGGDEDLGGSKAKKANRFKPV
                     VIYLGPFKLEKGQAKIHHIKMPNYVGSVRTMVVAGDINSSAYGSAEKTTPVRKPLMVL
                     ASLPRKISPSEKVTLPVTIFAMESKVKNVSVQIKTNNGVRVMGSSSQNLTFTQPDEKM
                     AYFNLEVGSATGLAKVQVIATSGSERATYEVELDITNPNPVTSEFTDVVLEPNSSKTI
                     DVSTFGVAGSNKAQLEVSSFPTIDFSRRLQYLIQYPHGCLEQTTSSVFPQLYLADIFD
                     LDQNRKTEIQKNIKIGIQKLGGFQIANGGFSYWSGQNYADDWSSSYVGHFLIEAEKKG
                     YVLPLNFKTKWIGYQQKASKEWRFAKNGNDFAQAYRLYTLALAGSPDMASMNRLRETI
                     GISNESKLRLAAAYALAGQKQAAQSVFNQSKIDDTNPNSYYYYGSPERNKAMALETLI
                     LIGNKQRAFKMATDLAKNMASDQWMSTQTTAYSLYAMSKFTQQNGGKGVEVNFSVNGK
                     PQSIKTAKSLANRNLEIKTGSNSITIKNTKSNTLYVRVLTSGILPIGQEKAMQSKLFT
                     NLVFKSRSGAPVNVTKITQGTEFIAEITIRNQTNERVENLALTQIIPSGFEIVNTRFT
                     DFGSFGNNVADHIDIRDERTNFYFGLKANESRTFRVLLNASYLGRYYLPGMQAEAMYD
                     NTYA"
BASE COUNT         1491 a         1081 c          914 g         1877 t
ORIGIN      
        1 gcatacgtgt tatcatacat cgcttcagct tgcataccgg gtaaatagta gcgacctaaa
       61 taagaagcat tcaacagcac tcggaacgtt ctggactcgt tggctttgag accaaaatag
      121 aaattggttc tctcgtcacg aatatcgata tggtcagcta cattgttgcc aaaactgcca
      181 aaatcggtga aacgggtgtt tacaatttca aatccggatg ggataatttg ggttaatgcc
      241 agattttcca ctcgttcgtt ggtttggttt ctaatggtga tttcggcaat aaactcagtg
      301 ccttgagtaa ttttagtcac gttcaccgga gctccacttc tgcttttaaa gaccaaattg
      361 gtaaacaact tactttgcat tgctttttcc tgaccaatcg gtaaaattcc gctagttaac
      421 actcgcacat ataacgtatt ggatttggtg ttttttatgg tgatggaatt gcttccggtt
      481 ttgatttcca aatttcggtt tgccaacgat ttagcggttt tgatgctttg cggtttgcca
      541 ttcacagaaa aatttacttc cacacctttt ccaccgtttt gttgagtaaa tttggacata
      601 gcatataaac tataagccgt ggtttgggtg ctcatccatt ggtcggaagc catgtttttg
      661 gccaaatcgg tcgccatttt gaaggctctt tgtttgttgc caatcaaaat tagcgtttcc
      721 aatgccatcg ctttattgcg ttcaggcgaa ccataatagt agtaactatt tggattagta
      781 tcgtcgattt tactttggtt aaagacactt tgtgccgctt gtttttgccc tgccaacgca
      841 taggcggcag ctaaacgcaa tttactttcg ttggaaattc cgatagtttc ccgcaaacgg
      901 ttcatcgaag ccatgtcggg cgaacctgcc aaggccaacg tgtataaacg ataagcttga
      961 gcaaaatcgt ttccgttttt ggcaaatcgc cattcttttg acgctttttg ttggtaacca
     1021 atccatttgg ttttgaaatt gagtggcaac acatagcctt ttttctcggc ttcaataagg
     1081 aaatgcccca cgtaggacga actccaatcg tctgcataat tttgtccact ccaataggag
     1141 aatccaccat tggcaatttg aaaacccccc aatttctgga taccgatttt gatgtttttc
     1201 tgaatttcgg ttttacggtt ttgatccaaa tcgaaaatat ccgccaaata caattgcggg
     1261 aacacactcg acgtggtttg ctctaaacaa ccatgtggat attgaatcaa atactgtaat
     1321 cttctgctaa aatcaatcgt cgggaaagac gaaacttcca attgagcttt gttacttcca
     1381 gcgactccga aagttgaaac atctattgtt tttgaactat tcggttccaa aactacatcg
     1441 gtaaattcag aagttaccgg atttgggtta gtgatgtcta attctacttc gtaggttgct
     1501 ctttcgcttc cggaagtggc gataacctgt actttagcta atccggtggc actgcctact
     1561 tccaaattaa aatacgccat tttttcatcc ggttgagtga aagttaagtt ttgagaactg
     1621 cttcccatta cccgaacacc attattggtt ttgatttgta ccgaaacatt tttaacttta
     1681 gattccatcg caaaaatggt taccggaagt gttacttttt ccgaaggaga aatttttctt
     1741 ggtaacgaag ccaagaccat taaaggtttt ctcaccggcg ttgttttttc agctgaaccg
     1801 taagcacttg aattaatatc accagccacg accatcgttc gaacagaacc aacataattt
     1861 ggcattttga tatgatgaat cttggcttgc cctttttcta atttgaatgg gccaaggtaa
     1921 attacaaccg gtttaaaacg attggctttt ttggctttgc ttccgcctaa atcttcatca
     1981 cctccgatgc tgaaaacttg gttaattcgt ccaccatacg ctcctattat ctcatcaaaa
     2041 atatcccaag tttttacccc taatgcatct cgggtgtaga aactgttcca agcattcggg
     2101 gttttgaaac gggttagatc caataaacca tcatctacaa ttgccaaggt atacgtcatc
     2161 gcttttcctg atttttcgct tacgcggatg ttcacttttt cttccggttt caagatttta
     2221 ggcatactga tttgtggttc tataatcgta tttttatcga ttacttctat cggcacaata
     2281 ccgtacaaac gaatcggtga atcatttaac gtggacgcat gcggttgcaa caacgtgatg
     2341 tgaacaaaca cgttaggagc catgtcgccc gtaatcggaa tttctacttt ggtctctcct
     2401 ttttgtgtgg ttgcccagaa ggtttttacc acacgagagc cattttctaa tgaaattaat
     2461 gctcttccac cggcactgga gggaaaggat attttagctt tttcgccaac agcataattt
     2521 tttttatcgg tggaaaacat caacatcgaa gcattagaag catcggtatt tctggttttt
     2581 ccggaccaat atggccaatc aatcattacg gtagaaccgg tagcgtgtcc acctgtttca
     2641 tcagttaccc gaataagata acgaccccat tcattttctt catttttaaa ttgaacgctt
     2701 gcttttccac ttgcatttgt actaactgca aaattcttat aggacgaagt tgaattatta
     2761 gaaacataac tggaaaggtt gtcataactc gcatcccacc accatctcca ttccacttta
     2821 taaacctgaa ctataaggtt ctgtactgct ttcggatttc ctttttcatc tacggttaca
     2881 atatcgtagc ggttcatttt tccggtttct aacaaaccat atttatttgg atcgggtgtt
     2941 tttaaaccta catatgtttt atacggtgaa agtgtcgcca ccatcacatc tgcactgaaa
     3001 tcaccaccat tttcatagac ttttgtcatt aaactggctt ttaacatacc cggtgattgg
     3061 ctacttactt taggttgaaa atttacggtt gcctttcctt cactattcac ttttccagta
     3121 aaaacagttg tctcttccgt ttcaaattga cggattgggt catcaaaatt ataattggca
     3181 aatcctttaa aagtggtttt ttgttgcatg attttagctt gcatttccat ttttaaatct
     3241 tttgcaatag caccatgtaa ccaagtcact tcaacttgac ttgtatttat tttggaggtt
     3301 gataaaattt cagaatcaaa tttattttta atttttagcc gattgggttt aatagtttca
     3361 atcttgatac ttttataaaa ctttgctcca cccacactga taactgcttc ccaatttcct
     3421 gtaggatgac tatcccgagt gggaataata aatttgtaat gattcaggtc attatatttc
     3481 tgcaccgatt gaaaggttaa ttttccgttt ggatcattaa gtttgaaact aataggatga
     3541 gatttatcca atttgctcgc agcatcattc aacataaaac tcaaaaacaa ggtgtcgcca
     3601 ggtcgccaaa cgcctctttc accataaata taacctttga gtcctttttg taatttttcg
     3661 ccggaaacat caaaattact tacggaaagc gaatagcctt cgtctaattt tacataggta
     3721 gattgtttcc cttttgttac tatggcaaaa taggcatatt tgttcaattc gaatttagta
     3781 attccttcta catttgttat acctgatgct aatttttgtt gttgaaaatt atacagttca
     3841 actgttgcac ctgaaaccgg ctgggtgtta acaatatttg taaccgcaaa aagataagaa
     3901 ccgttttctc ctcttttagc aataacccct aaatctgaag ccagtacatt catcccgatt
     3961 ggccctcgac tataataatc attttcgcaa ggatctttgc tgtcgtacca atcgtaatca
     4021 tcataccaat aataatcata ataatcatca tcgttagaac gcacttcttc ggattcttct
     4081 tcctctgatt ccgtttcagt agttgaattt gaacatttat acaaagaata actctttttg
     4141 aaagacaatt caacacgata gatggctccc ggctccggag tgataacttt ggctaaatcc
     4201 aaagcaaaac tgttccactt gctgtaattg ggtaattttt tatctattaa attgattgtc
     4261 tgttttgcta cgggtgtggc aacttttctc agatttcttt tcccatttaa gtcattgtct
     4321 tgtaaaaatt gaaggatatt attcttataa attttataaa tttttacgtc aactgctttc
     4381 aaattagctg cttcaaagtt aatttttaaa ttattggaac ttggtaaaat tgttccgttt
     4441 ttcatgaaac ggatacccgg ttttatttca ttgaaggcaa ctttttgtgc atgtgtttct
     4501 ttcattttgt agccttcctc gctttcgata ccttggaaaa tttccactaa taaatctcct
     4561 ttaagtggtt cactaaaaaa cactttcaat atatttcctt cgatagaaaa tttattagca
     4621 atagcagttt ctactgaaac taatcctctt aaatcctgcc cttttttaat agggtcagaa
     4681 aaattgataa gcaacgactg attatcatca tcgccaacct ctaagcccac aattttaaaa
     4741 ttatttttac ctggaatctc aaaatctacc attccttttt ggttgatgtc aaatggattt
     4801 ccattccatg aaattttaat tttactatct tcgacttccc gtttaatgct gtcaatgata
     4861 aatttaaatt cagttcctcg gcttaattct ttatcaaatt ttatttttaa tttagttccg
     4921 ttttgagacg cttctactaa cttagacgcg gtttcaaaac tcatttgatc actggtttga
     4981 atggttccgt tcaaaaattg ccattctctg ttatacgact gtaaatccaa tacattaact
     5041 ataaaatcct gtttcagtgt tttaatggag aacttaaatt cagaaaggtt ttttggtgtt
     5101 ttgataagtt tggataattt gaaaataatc cggtattctg tatcttgttc taaaggtttt
     5161 tcgggaataa acgctatggt attagaagcc aatgccacca tttttccttt tacagcagga
     5221 gatatatcaa ataaactttg atcaatttct tgattaggtt gccattcagc tttatcaaaa
     5281 gctagcgtga ctcgaatatc agtatttgat gaaaccaatc ctgaactgaa attcagaaca
     5341 tagtccttga acaatgaaaa atc
//
LOCUS       DAOTCW010000151         5332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_11616_length_5332_cov_31.884594, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000151 DAOTCW010000000
VERSION     DAOTCW010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11616_length_5332_cov_31.884594"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="P5335_10145"
     CDS             <1..581
                     /locus_tag="P5335_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRZ75282.1"
                     /translation="VFIEKVEANPFVKVVYDGIDIQSNLIGLYNATNIIAAITVGKYF
                     EVALNDIKRGIESYMPENNRSQIIFKNGNEIILDAYNANPSSMDVALDNFIQLKKANK
                     VIIIGDMFELGDESKKEHQTIVNKLISQVDFTCFLIGKEFYDSKIENKYLSFFLSFED
                     FSNFLKENKFENDFILIKGSRGMALERTLDFL"
     gene            complement(586..660)
                     /locus_tag="P5335_10150"
     tRNA            complement(586..660)
                     /locus_tag="P5335_10150"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(623..625),aa:Val,seq:gac)
     gene            complement(701..775)
                     /locus_tag="P5335_10155"
     tRNA            complement(701..775)
                     /locus_tag="P5335_10155"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(738..740),aa:Val,seq:tac)
     gene            complement(845..2065)
                     /locus_tag="P5335_10160"
     CDS             complement(845..2065)
                     /locus_tag="P5335_10160"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRZ75283.1"
                     /translation="MNYQETVNWLFNRLPMYQQQGASAYKADLTNTILLANYLGNPEK
                     KLKTIHVAGTNGKGSTSSLLASVLQEAGYKVGLYTSPHLKDYRERIKINGKEISEKFV
                     VDFVSKHKPFFESNDLSFFEMTVGLAFDYFVQENIDIAIIEVGLGGRLDATNIIKPLI
                     SVITNIGLDHTQFLGNTIEAIAKEKAGIIKPLSPIVIGEYTAETKPIFAATAKENNAE
                     IYYAADLIKEIYPTELLGDYQFQNRKTVLQTLQVLQNQNEYTISEKNIKDGFMKVIQN
                     TGLLGRWQQVNSRPKVICDTAHNKHGLTIVINQIKKEKYENLHIVLGVVNDKDLNDIL
                     PLFPKNATYYFCRPNITRGLNEKLLKEKALTFELIGKVYLSVSEAYNNALKNASPNDF
                     IYIGGSTFVVAEIL"
     gene            complement(2125..2961)
                     /locus_tag="P5335_10165"
     CDS             complement(2125..2961)
                     /locus_tag="P5335_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HRZ75284.1"
                     /translation="MKFLETEYEKKSFALSASITIGLLLLFFVFGLTYMDPPPENGIA
                     INFGNTDAGSGNTNTTELVKSELREVTEQPKVEETSEPTTPSKASENVLTADNDAAIA
                     LKKQKEIADAKASAEAKIKAAEQKKKQEEADKKAKLDALIGGVKKSEGKTTGGDGTST
                     TPGNQGKLDGSIYSNSYYGSGKGTGTGNGSWGLNGRKLSKPGAVKADCNDEGTVVVEV
                     KVSKNGTVTNAKFSPSGSNTSSKCLQDAAIKSAYKYKWNDDSNAPDNQIGFIVFNFRN
                     GE"
     gene            complement(2970..3362)
                     /locus_tag="P5335_10170"
     CDS             complement(2970..3362)
                     /locus_tag="P5335_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRZ75285.1"
                     /translation="MNIRGRNKVSPEFNMSSMTDIVFLLLIFFMLTSTMVTTNALDLV
                     LPKAKGKTDSNKNISVSINKDLEFFIDKDQVQEADLEAKLLALFPNKEDKAIILRAEE
                     GVPIEKAVNVLDIANRNQIKVVLAVRPK"
     gene            complement(3364..4059)
                     /locus_tag="P5335_10175"
     CDS             complement(3364..4059)
                     /locus_tag="P5335_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRZ75286.1"
                     /translation="MQAIDSLATTQNAVLDPQPIEKTLSIWELVSSGGIGGQMIMITL
                     GLLFFFALYLYFERLMAINAASKIDVNFMNNIKMNIMNGKIDAAKMLCAQTNSPVARL
                     TEKGISRIGKPLEDINTAIENAGKLEIYKLEKNVSMLATISGAGPMIGFLGTVIGMIL
                     AFHKMASGGGQIEVGALAEGIYTAMTTTVAGLVVGIISFMGYNHLVVKTDKVVHQMEA
                     NATDFLDLLNEPS"
     gene            complement(4107..>5332)
                     /gene="nhaD"
                     /locus_tag="P5335_10180"
     CDS             complement(4107..>5332)
                     /gene="nhaD"
                     /locus_tag="P5335_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HRZ75287.1"
                     /translation="TVIDGYGSVFNIASGEIHQQEHGFENLLLHHLGKTAEIIVFLIG
                     AMTIVELIDLHRGFDVLKGMVKTTSKIKLLWITGIIGFLLSSVIDNLTATIVLISLLR
                     KLIQNREERIWYVSLIIIAANAGGAWSPIGDVTTTMLWIAKKVSAVGLSEYIIVPAVI
                     CFVVPFALASRMKIFKGDIQIEIKNNEVEQKLLSSKTMLWVGLGAIIFVPIFKTLTHL
                     PPYVGMMFSLAIVWLVSEYIHPEDHFDKSKKHLYSAQKALSRIEISSILFFLGILMAV
                     AALESLVFGTIQGQEVGTLRYAAESISNAIPNMDVVIILLGILSAVIDNVPLVAASMG
                     MYTYEIDHSAWHFIAYSAGTGGSMLIIGSAAGVAAMGMEKIDFIWYLKKITWLAFAGF
                     IAGAIAFLFIEHFVR"
BASE COUNT         1682 a          938 c          803 g         1909 t
ORIGIN      
        1 atgtttttat tgaaaaagtt gaagcaaatc cttttgttaa agttgtttat gatggaatag
       61 acattcaatc taacttaata gggttgtata atgctacaaa tattattgca gcaatcacag
      121 ttgggaaata ttttgaagtt gcattgaatg atattaaaag aggtattgaa tcttatatgc
      181 cggaaaataa tagatcacag attatattta aaaacggaaa tgaaatcatt ctagatgcct
      241 ataatgctaa tcctagcagt atggacgttg ctttggataa ttttattcaa ttaaaaaaag
      301 caaataaagt tataattata ggtgatatgt ttgagctagg agatgaaagt aaaaaggagc
      361 atcaaacaat tgtaaataaa ttaatttcgc aagttgattt tacttgtttt ttaatcggaa
      421 aagaatttta tgattctaaa attgaaaata aataccttag ttttttcctt tcatttgaag
      481 atttttcaaa ttttttaaaa gaaaataaat ttgaaaatga ttttatcctt ataaaaggtt
      541 ccagaggaat ggctctagaa cgaacattag attttttata aaaagtgggt catattggat
      601 tcgaaccaat gacttccacg ttgtcgacgt gacactctga accaactgag ttaatgaccc
      661 ttttgaataa aaaaactccc taatttctta aggagttttg tgggcgatga ggggttcgaa
      721 cccccgaccc cctcggtgta aacgaggtgc tctgaaccag ctgagctaat cgcccgatta
      781 atgcttgatt gcgtgtgcaa atatagaaca gttttttgtt tctacaaata aaaatgtaaa
      841 aaaattataa aatttctgct acaacaaaag tactgccacc tatataaata aagtcatttg
      901 gactggcatt ctttaatgca ttattataag cttctgaaac agaaagatat acttttccga
      961 ttaattcaaa tgtcaaagct ttttccttta ataatttttc gtttaaacca cgtgtaatat
     1021 tgggtctaca aaaataatag gtagcatttt taggaaataa aggtagaata tcatttaaat
     1081 ctttatcatt gactactcca agaacaatat gaaggttttc atatttctct tttttaattt
     1141 gatttattac aattgtaagg ccatgtttat tatgagctgt atcacaaatc acttttggtc
     1201 tcgaatttac ttgttgccat ctgcctaata gtcctgtatt ttgtatcact ttcataaatc
     1261 catcttttat gtttttttct gagatggtat attcattttg attttgcaaa acttgaagtg
     1321 tttgcagtac tgtttttctg ttttgaaatt gataatctcc taacaattca gttggatata
     1381 tttcttttat taaatcagcg gcataataaa tttcagcgtt attttctttt gctgttgcag
     1441 caaaaattgg ttttgtttca gcggtatatt ctccaattac aataggtgat aaaggtttta
     1501 ttattcctgc tttttctttt gcaattgctt ctattgtatt gcctaaaaat tgtgtgtgat
     1561 ctaaaccaat atttgtaatt acagagatta atggtttaat aatattagta gcatccagtc
     1621 tgcctcctaa tccaacttca ataatggcaa tgtcaatatt ctcctgaacg aaatagtcaa
     1681 aggctagtcc tacagtcatt tcaaaaaaac ttaaatcatt tgattcaaaa aaaggtttat
     1741 gttttgaaac aaaatcaact acaaattttt ctgaaatttc ttttccgttt attttaattc
     1801 gttctctgta atcttttaaa tgaggagaag tatacagacc aactttataa ccagcttctt
     1861 gtaggactga agctagaaga gaagacgttg agccttttcc attcgttccg gctacatgaa
     1921 tggtttttag ttttttttcc ggatttccta aataatttgc aagaagtata gtgttagtta
     1981 aatctgcctt ataagcagat gctccttgtt gttgatacat tggaagtcgg ttaaagagcc
     2041 aatttacggt ttcctgataa ttcattgttt tcaaaaaata aaccccacaa aagtgaggtt
     2101 tgtagctatg taatttttgt ttttttattc tccgtttcta aagttaaata cgataaatcc
     2161 aatttgattg tcgggagcat tagaatcatc attccacttg tatttgtatg cagatttaat
     2221 ggctgcatct tgaagacatt tactagaagt atttgagcca cttggactaa atttggcatt
     2281 agttactgtt ccatttttgc ttacttttac ttccacaaca accgttcctt catcattaca
     2341 gtctgctttt actgccccgg gtttagatag ttttcttcca tttagtcccc aacttccatt
     2401 tcccgtacct gttccttttc cgcttccata ataactattt gaataaatac taccatccaa
     2461 tttcccttgg tttcctggtg ttgttgaagt tccgtcacca ccggttgttt taccttctga
     2521 tttttttacg cccccaatta aggcatctag ttttgctttt ttatctgctt cttcctgctt
     2581 ttttttctgt tcggcagctt ttatttttgc ttcggcactt gcttttgcgt cagctatttc
     2641 tttttgtttt tttaaagcaa ttgcagcatc attatcggct gtaagaacat tttctgatgc
     2701 tttacttggt gttgtaggtt ctgaagtttc ttctactttt ggttgctcag tgacttctct
     2761 tagttcagat tttaccagtt ctgttgtgtt tgtgtttcct gaaccagcat cggtgttacc
     2821 aaagttaata gcaattccgt tttccggtgg aggatccata taggttaacc caaatacaaa
     2881 aaacaacaat aacaatccaa ttgtaattga agcacttaat gcaaatgatt ttttttcgta
     2941 ttcagtttct aaaaacttca tgactaagtt tatttaggtc gaacggctaa aactacttta
     3001 atttgatttc tattggcaat atccaaaaca tttactgctt tttcaatggg aacaccttct
     3061 tcagctctta aaatgatagc tttatcttct ttatttggaa acaatgctaa caatttagct
     3121 tctaaatcag cttcctgtac ttgatcttta tcaataaaaa actctaaatc tttattgata
     3181 cttactgaaa tatttttgtt gctgtctgtt tttccttttg cttttggcaa taccaagtcc
     3241 aaggcatttg ttgttaccat ggtggatgta agcatgaaga aaatcaaaag caagaataca
     3301 atatccgtca tagacgacat attaaattcc ggtgatacct tatttctacc tctaatattc
     3361 atattaagat ggttcgttta aaaggtctaa gaaatcagtt gcatttgctt ccatttgatg
     3421 caccactttg tcagtcttta ccaccaaatg attatagccc ataaacgaaa ttatacctac
     3481 tactaatccg gctactgttg ttgtcatggc agtataaatc ccctcagcta aagctcctac
     3541 ttcaatttgt ccacctccgc tcgccatttt atgaaaggca agaatcatac caattacagt
     3601 tcctaaaaac ccaatcattg gccctgctcc ggaaatggta gctaacatac tgacattttt
     3661 ctccaattta tatatttcta atttacccgc attttcgatt gccgtattta tgtcttctaa
     3721 aggttttcca attcgggata ttcctttttc agttagtctg gcaaccggag aattggtttg
     3781 tgcacaaagc attttggcag catcaatttt tccattcata atattcattt taatattatt
     3841 catgaaattc acatcaattt ttgaagcggc attaattgcc atcaaacgtt caaaataaag
     3901 atataaagca aagaaaaata acaatcctaa tgttatcata atcatttgac caccaattcc
     3961 accacttgaa acaagttccc agatggataa tgttttttca atgggttgag gatctaaaac
     4021 tgcattttgt gttgttgcta aactatctat tgcttgcata ttatttgatt taaattaagt
     4081 aacgctaaaa tactgtttta aattgtttaa cgaacaaaat gctcaataaa caaaaacgct
     4141 attgctccgg caataaaacc ggcaaaagcc aaccaagtta tctttttaag ataccaaatg
     4201 aaatctattt tctccattcc catggctgca acacctgctg ctgagccaat aattaacata
     4261 cttcctccgg ttccggctga ataagctata aaatgccatg ctgaatgatc aatttcgtaa
     4321 gtatacattc ccatggacgc ggcaacaaga ggtacgttat caatcacagc tgataagatt
     4381 cctaataaaa ttataacaac atccatattt ggaatggcgt tactaatact ttctgctgca
     4441 tagcgaaggg tccctacttc ctgaccttga atagttccga atactaaaga ttctagtgct
     4501 gcgactgcca ttaaaattcc taaaaagaat aaaatacttg aaatttcaat tcttgatagg
     4561 gctttttgag cagaatacaa atgttttttg cttttatcaa aatgatcttc aggatgaata
     4621 tattccgaaa ctaaccatac aatagctaaa ctaaacatca tacctacata aggaggtaaa
     4681 tgggttaatg ttttaaaaat tggcacaaaa ataatagcac ctaaacctac ccaaagcatg
     4741 gttttactac ttaaaagttt ctgctccact tcattgtttt taatttctat ttgaatgtct
     4801 cctttaaaaa ttttcattcg agaggctaat gcaaaaggga ctacaaaaca aattactgct
     4861 ggaacaatta tatattctga taaaccaacg gctgatactt ttttagcaat ccaaagcata
     4921 gttgttgtaa catctccaat aggtgaccat gcacctccag catttgcagc aataataatc
     4981 aaggaaacat accaaatgcg ttcctctcta ttttgaatta atttacgcaa taaagatatt
     5041 aaaacgattg tagcagttaa attatcaatt acagaggaaa gtaaaaatcc aataattccc
     5101 gttatccata aaagtttaat tttacttgta gtttttacca tgccttttaa cacgtcgaaa
     5161 ccacgatgta aatcaattaa ttctacaatt gtcattgccc caataagaaa gacaataatt
     5221 tcagcggttt tgccaaggtg atgtaaaagt aaattttcaa aaccatgttc ctgttgatga
     5281 atttcaccgg aagcgatatt aaaaacggag ccataaccat caattacggt aa
//
LOCUS       DAOTCW010000152         5328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_11628_length_5328_cov_36.078324, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000152 DAOTCW010000000
VERSION     DAOTCW010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11628_length_5328_cov_36.078324"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="P5335_10185"
     CDS             <1..830
                     /locus_tag="P5335_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HRZ75288.1"
                     /translation="TAPFPNRDGFNYVIAAVDIDGKQLLLDATDANLAINILPIRCLN
                     GTGRLIRQNGTSEEIQLNPTFLSKNTTMIQGELESNGSFKGKLRTSKTDYLAFAFRDD
                     FMNLNEETYLEKLEEDYHDLVISEYKLENKNAVDKPIVESFSFVEANSCEFIGSKMLF
                     NPLLFLTKSINPLKAETRKLPIDFIFPQSDKTIINIFIPIGFEVEYLPKSITLTTPDN
                     GAVYKFSIQSEKNKIQITTSLDLNSIFFDPQEYQDIKKIFNTVAEKNTEKIILKKSE"
     gene            830..1156
                     /locus_tag="P5335_10190"
     CDS             830..1156
                     /locus_tag="P5335_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRZ75289.1"
                     /translation="MNLKNAQLDVDTWIKNHGVRYFNELTNMAQLTEEVGEVARIIAR
                     RYGEQSEKESDKNKDLGEELADVVFVVLCLANQTGVDLQDAFDKKMDLKTKRDHDRHQ
                     NNEKLK"
     gene            1158..1484
                     /locus_tag="P5335_10195"
     CDS             1158..1484
                     /locus_tag="P5335_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HRZ75290.1"
                     /translation="MNWILLIIAGLFEVAFATCLGKAKETSGTTMWLWYGGFLVCLSI
                     SMLLLVKATKELPIGTAYAVWTGIGAVGSVIVGIVVFKEPATFLRVFFISTLIISIIG
                     LKVVTK"
     gene            1484..2725
                     /gene="aroA"
                     /locus_tag="P5335_10200"
     CDS             1484..2725
                     /gene="aroA"
                     /locus_tag="P5335_10200"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRZ75291.1"
                     /translation="MDIKLTYNQPPATFKPQLKITGSKSVTNRLLLLQALYPNIIIRN
                     GSISDDSEVMIQALQNQPPINSHPQLINIHHAGTAMRFLTAFFAIQEGKEVILTGSSR
                     MKERPIKILVDALNQLGAEITYEENEGFPPIRIIGKKLAQNKVSLPANVSSQYISALL
                     LIAPKLKNGLELTLEGEMTSVPYVKMTLTLLNNLGIQTTFNHHKIIISSKDKVKNTEF
                     SVESDWSSASYFYSIVALSPRNTKIELATYHKNSLQGDFKLIEIYKNLGVETNFTETG
                     ISLEKVNGVHQSIKLNLKDCPDIAQTIAVTCFGLGVGCNLEGLHTLRIKETNRLEALR
                     VEFSKLGAMIITTDTGLIIAPSTKINENISINTYQDHRMAMAFTPLAIKVPIIIKDSE
                     VVSKSYPSFWEDLESIGFQIS"
     gene            2908..3957
                     /gene="queA"
                     /locus_tag="P5335_10205"
     CDS             2908..3957
                     /gene="queA"
                     /locus_tag="P5335_10205"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRZ75292.1"
                     /translation="MKLSHFQFNLPTELLAEYPAENRDESRLMVINRAKKTIEHKLFK
                     DIIDYFDDGDVMIMNNTKVFPARLYGNKEKTGARIEVFLLRELNAEQRLWDVLVDPAR
                     KIRIGNKLYFGDDDSLVAEVIDNTTSRGRTLRFLYDGSYDEFRNKLTELGETPIPKYI
                     SREVTPEDEERYQTIYAKVEGAVAAPTAGLHFSKHLMKRLEIKGVDFAEVTLHVGLGT
                     FNPVEVEDLSKHKMDSEELIIKQNACDVVNNAISNKKRVCCVGTTSMRAIESSVSSAR
                     TMNPFEGWTNKFIYPPYDFSIANCMITNFHTPKSTLLMMVSAFCGHDLMKKAYEEAIK
                     EKYRFYSYGDAMLII"
     gene            4146..>5328
                     /gene="kynU"
                     /locus_tag="P5335_10210"
     CDS             4146..>5328
                     /gene="kynU"
                     /locus_tag="P5335_10210"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HRZ75293.1"
                     /translation="MIFENTRELAQQLDAQDELFNYREEFIFPKVNGKKVIYFTGNSL
                     GLQPKRTKAYVDEVMNDWANLAVEGHFYADKPWWDYHERFAAPLSEMVGAKPIEITVM
                     NTLTVNLHLMMVSFYRPTPKKYKIICEEKAFPSDQYMFESQAQFHAQYLGINPKDIIV
                     EIKRREGEHNIRLEDVLAEIEEVGDELALVLIGGVNYYTGQVFDMKTITAAAQKTGAY
                     VGWDLAHAAGNVELELHDWNVDFACWCSYKYMNSGPGNASGCFVHERHHGDKDLPRFA
                     GWWGHNKERRFKMEPNFDPVHGADGWQISNLPILSLAPYLASVEMFAEVGMKKLVKKR
                     KLLTAYLEFILHEIDREIDGTEFEIITPLNQEERACQLSVYLHGQGRSLFEYLMKNGV
                     IT"
BASE COUNT         1861 a          859 c          957 g         1651 t
ORIGIN      
        1 gtacggcccc attccctaac agagatggat ttaattatgt tattgcagca gttgatatag
       61 atggaaagca attattgtta gatgcaactg atgcaaattt agctattaat attttaccta
      121 ttcgttgttt gaacggaact ggtagattga ttcgacaaaa tggaacctct gaagaaattc
      181 aactaaaccc aacatttctc tcaaaaaaca ccacaatgat tcaaggtgaa ttagaaagta
      241 atggaagttt taaaggcaaa ttaaggacat ctaaaacaga ttatttagct tttgcttttc
      301 gtgatgattt catgaattta aatgaagaaa cctatcttga aaagttagaa gaagattatc
      361 atgatttggt gatttcagag tataaattag aaaacaaaaa tgcagttgac aaacctattg
      421 tagaaagttt ttcatttgtt gaagcaaatt catgtgagtt tattggttca aaaatgctat
      481 ttaatccatt attgttttta acaaaatcga tcaatccgtt aaaagcagaa actagaaaat
      541 tacccattga ttttattttt ccgcaaagtg acaaaactat cataaatatt tttattccga
      601 taggttttga ggttgaatat ttgccaaaat caattacatt gactactccg gataacggag
      661 cagtttataa attttctatt caaagcgaaa aaaataaaat tcaaataaca acatctttag
      721 atttaaattc aatatttttc gacccacaag aataccaaga cataaaaaaa atatttaata
      781 ccgttgcaga aaaaaatact gaaaagataa tccttaaaaa aagcgaataa tgaacctcaa
      841 aaatgctcaa cttgatgtcg atacctggat taaaaaccat ggtgttcgtt attttaacga
      901 attaacaaat atggcccaac ttacggaaga agtaggggaa gtcgctagaa taattgcccg
      961 tcgctatggg gaacaatccg aaaaagaaag tgataaaaat aaagatttag gggaagaatt
     1021 agccgatgta gtttttgttg tattatgttt ggctaatcaa accggagttg atttacaaga
     1081 tgcttttgat aaaaaaatgg atttaaaaac caaacgtgat catgacagac atcaaaataa
     1141 tgaaaaattg aaataaattg aattggatac tactcattat tgcaggttta ttcgaagtag
     1201 cttttgccac ttgtttggga aaagcaaaag aaacttcagg aacaaccatg tggctgtggt
     1261 atggcggatt tttagtttgc ctctcaatca gcatgttgct tttagttaaa gcgacaaaag
     1321 aattgccaat tgggacagct tatgcggtgt ggacaggaat tggagctgtt ggtagcgtta
     1381 tagttggaat agtagttttc aaagaacccg ctactttttt gagagtattt tttatcagta
     1441 ctttgatcat ttcaattata ggactaaaag ttgtcactaa ataatggata taaaattaac
     1501 ctacaaccaa ccacctgcta cttttaagcc gcaattaaaa atcaccggtt cgaaatccgt
     1561 aacaaatcga ttgttgttac ttcaagcttt gtatccaaat attatcatta gaaatggttc
     1621 tatttccgat gattcagaag taatgattca agcattgcaa aaccagccac ccattaacag
     1681 tcacccacaa ttaataaaca ttcatcacgc cggtacagcc atgcgatttc ttaccgcttt
     1741 ttttgccatt caagaaggaa aagaagtaat tttgacaggt tcctccagaa tgaaagaacg
     1801 accaattaaa attttagtgg atgcattaaa tcaattggga gcagaaatta cgtatgaaga
     1861 aaacgaaggg tttccgccca ttcgcatcat aggaaagaag ttagcacaaa ataaagtttc
     1921 acttccggct aatgttagta gtcaatatat ttccgctctt ttgttaatag ctccaaaact
     1981 gaaaaacgga ttagaattaa cacttgaagg agaaatgaca tctgttccct atgtcaaaat
     2041 gacattgacg ctattaaata atttgggaat acaaacaact tttaatcatc ataaaattat
     2101 tataagttca aaggacaaag ttaaaaatac agaattttca gttgaatccg attggtcttc
     2161 tgcttcttat ttttattcta ttgttgcttt gtctccaaga aatacaaaga ttgaattagc
     2221 tacctatcac aaaaatagtt tgcaaggcga ttttaaatta attgaaattt ataaaaattt
     2281 aggtgtagaa accaatttta cagaaacagg aatatcactt gaaaaagtaa atggtgttca
     2341 tcaatcaata aaactaaatt taaaagattg tcctgatatt gcacaaacga tagcagttac
     2401 ttgttttgga cttggtgtgg gttgtaatct tgagggttta catactctaa gaattaaaga
     2461 gaccaataga cttgaagctc taagagtaga attttcaaaa ctaggtgcaa tgattattac
     2521 aactgacacg ggattaataa ttgcaccatc tactaaaatt aatgaaaaca tttccatcaa
     2581 tacctatcag gatcacagaa tggcaatggc ttttactcct cttgccataa aagttcctat
     2641 tatcattaaa gattccgaag tggtttcaaa atcgtatcca tcattttggg aagatttaga
     2701 gagcattgga tttcaaattt cctaaaggag tataggttta tcagtaatga aatctctcat
     2761 aaccttagtt tcttagcacc ttaacatctt tctgaaaaaa taaacactaa aacacttgac
     2821 aacccctatc tcacaaccgt atatttgcag gctttaagtt tttaagtatt tacttaaaaa
     2881 ttcgaacatt aaacaaaaaa taagtacatg aaattatctc attttcaatt taatcttccg
     2941 acggaattat tggcagaata tccagctgag aaccgcgacg aatcacgatt aatggttatc
     3001 aacagagcca aaaaaacaat tgaacataaa cttttcaaag atatcattga ctattttgat
     3061 gatggagatg tcatgattat gaataatacc aaagtttttc cagctcgttt gtacggaaat
     3121 aaagaaaaaa ccggggcaag aattgaggtt tttttattac gtgaattaaa tgcggaacaa
     3181 cgtctttggg acgttttagt tgatccggcc cgtaaaattc gtatcggaaa taaattatat
     3241 tttggtgatg acgattcatt agtagcggag gtaattgata atacaacttc ccgtggaaga
     3301 actttgcgat ttctttatga tggctcctat gatgagttca gaaataaact aacagaatta
     3361 ggcgaaaccc caattccaaa atacattagc cgtgaagtaa cccctgaaga tgaagagcgt
     3421 taccagacta tctatgctaa agtggaaggt gccgttgcag caccgactgc cggtcttcac
     3481 ttttctaaac acttgatgaa acgtctagaa ataaagggag ttgatttcgc agaagtaact
     3541 ttacatgttg gccttggaac attcaatcca gtggaagtgg aagatttatc taaacataaa
     3601 atggattcag aagagttgat tattaaacaa aatgcttgtg atgtggtaaa caatgccatt
     3661 tcaaacaaaa aacgcgtttg ttgtgttgga acaacttcta tgcgagctat tgaaagttct
     3721 gtctcttctg ctagaacgat gaatcctttt gaaggatgga caaacaaatt tatctatcct
     3781 ccttatgatt tcagtattgc aaattgtatg attactaatt tccacacacc aaaatcaact
     3841 ttattaatga tggtttctgc tttttgtggg catgatttga tgaaaaaggc ctacgaagaa
     3901 gcgattaaag aaaaatatag attctattct tatggcgatg ccatgttgat tatttaattt
     3961 gtctcgaaac ttcagtattg ctattgctaa tttcttttca atatttcaga ttctcatata
     4021 tgaattctcg gcctttggtt gggaattttt ttttaatata gctactgttt ttcgttggaa
     4081 gtagctttca attccgagtc ttaaatataa aaaattacta tctttacacc actaataaaa
     4141 aacccatgat ttttgaaaac acacgtgaat tagcacaaca attagatgct caagacgaac
     4201 tgtttaatta tcgagaggaa tttatcttcc ctaaagtaaa cggtaaaaaa gtcatctatt
     4261 ttacaggaaa ttcactagga ttacagccaa aacgaactaa agcttatgta gatgaagtaa
     4321 tgaacgactg ggcaaatctc gctgtcgaag gtcactttta tgccgacaaa ccttggtggg
     4381 attaccacga gcgttttgca gctcctttaa gtgaaatggt tggagctaaa cctatagaaa
     4441 ttactgtcat gaacacactt accgtgaatc ttcacttaat gatggtgtct ttttatcgtc
     4501 ccactccaaa aaaatataaa atcatttgtg aagaaaaagc ctttccttcc gatcaataca
     4561 tgttcgaaag tcaggctcaa tttcatgcac aatatttggg aattaatccc aaagatatta
     4621 tcgtagaaat caaacgacga gagggtgagc ataacatacg tctagaagat gttttagccg
     4681 aaattgaaga agttggagat gagttagctc tggttttaat cgggggcgta aactattata
     4741 caggtcaggt tttcgatatg aaaacaatta ctgccgccgc acaaaaaaca ggtgcttatg
     4801 taggttggga tttggctcat gctgcaggaa atgttgaact cgaacttcac gactggaatg
     4861 tggattttgc ttgttggtgt agttataaat atatgaattc aggtcctgga aatgcttcgg
     4921 gttgttttgt acatgaaaga catcacggcg ataaagactt gcctcgtttt gccggttggt
     4981 ggggacataa caaagaacgt cgttttaaaa tggaacccaa tttcgatccg gtacatggag
     5041 ccgatggttg gcaaatcagt aacctgccta ttttatcttt agctccttat ttggcttccg
     5101 tggaaatgtt tgcagaagtg ggaatgaaaa aattagtaaa aaaaagaaaa cttttaactg
     5161 cttatttgga attcattctg catgaaatcg accgcgaaat cgacggaacc gaatttgaaa
     5221 tcatcacccc tttgaatcag gaagaaagag cttgccaact ttcagtttat ttacacggtc
     5281 aaggaagaag tttattcgag tatttaatga aaaacggtgt gattaccg
//
LOCUS       DAOTCW010000153         5288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_11765_length_5288_cov_38.421173, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000153 DAOTCW010000000
VERSION     DAOTCW010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11765_length_5288_cov_38.421173"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="P5335_10215"
     CDS             complement(<1..1204)
                     /locus_tag="P5335_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRZ75294.1"
                     /translation="MKRKYNYFLLWLLHFFAFSVWGQEVSLYQQFNGRYDFTFIGNTL
                     NTEENRGILSPCIINTSATATLAMQPDDAIEKAFLYWAGSGPGDFEVQFNGISITPDR
                     TFNLIQSTSGLIFFSAFKDVTEQVLSTGNGDYTLSELDLTELIPDYCPNATNFAGWAM
                     IIVYKNDSLPLNQLNVYDGLDYVSFGNTNLTFVLDNLNVIDDIGAKIGFIAWEGDSNL
                     AFEETLQINGNILSNPPLNPENNAFNGTNSITGSSALFNMDLDIYDIQNNIQPGDQSA
                     EIALTSGQDFVMINAVVTKLNSQVPDATIVIDNVYLSCNSNEVTVDYTVYNLNATNPL
                     PANTPIAVYVNGVVIASAATMNVIAIDGSESNSITLLLPEGITNPFELVFAVDDIGNG
                     TGIVIEISD"
     gene            1278..2267
                     /locus_tag="P5335_10220"
     CDS             1278..2267
                     /locus_tag="P5335_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRZ75295.1"
                     /translation="MSHTVQQYLIRKYTSADYSIWNDFINQAKNATFLFHRDFMEYHH
                     DRFEDYSLIVEDEKGWLAVLPANRVGEEVFSHQGLTYGGLVFKGDLKLEKVLLIFKSI
                     LQQLHISEIKTLHIKELPFIYSDYFSDELKYALFLAEAKLSRRDTLAVIDLSKEIKLA
                     SGRMEGVKRGIKNELIIKEENDFTSFWNLVLIPNLINKHQANPVHSLEEISNLKQKFP
                     LNIRQFNVYKENEIVGGTTIFESKKVAHSQYISVTSSKNEYGTLDFLHHYLMTEVFKD
                     KAFFDFGISNEEQGRKLNGGLSFWKESFGAKIVTQDFYQVQTENYIQLEQVIV"
     gene            2264..>2736
                     /locus_tag="P5335_10225"
     CDS             2264..>2736
                     /locus_tag="P5335_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRZ75296.1"
                     /translation="MIKFFDLHKVNKQYEKAFHEKLEQFMQRGWYILGDEVQSFENQF
                     AAHCGTKHCIGVGNGLDALVLIFKAYLELGKLQKGDEVIVPANTYIASILSILQTDLV
                     PVLVEPKLETFNLNPDLIAAKITSKTKAILVVHLYGQLADMEKIDSIAKQHNLLI"
     assembly_gap    2737..2836
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2837..3370
                     /locus_tag="P5335_10230"
     CDS             <2837..3370
                     /locus_tag="P5335_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRZ75297.1"
                     /translation="KNLGCLGDGGAVLTNEEDLNKVIRALRNYGSEKKYVNDYLGINS
                     RLDEIQAGFLNVKLPNLANDNQKRRQIANRYLAEIKNDKIVLPFYDGSTNHVFHLFVI
                     RTEKRTELQDYLKENGIETMIHYPIPPHQQKALKPFNSLSFPITEKIHREVLSLPMSP
                     VLTEEEVSRIIQVLNQY"
     gene            3370..3942
                     /locus_tag="P5335_10235"
     CDS             3370..3942
                     /locus_tag="P5335_10235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016478846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRZ75298.1"
                     /translation="MGLFYKIKQLLFVEVRILKFKLLSDCKNTVGKPNLYHPLLLKGK
                     GSISFGNNVQIGVVASPNYFSHYAYLEARESNSAIQIGNNVSINNAFSAVAFSKILIG
                     DNVLIGTNCSLMDNDAHHLASNKRHESSISKEIVIENNVFIGSNVTILKGVTIGENTV
                     IGNGSVVTKSIPKNVIAAGNPAHVIRELPC"
     gene            3936..5213
                     /locus_tag="P5335_10240"
     CDS             3936..5213
                     /locus_tag="P5335_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen translocase"
                     /protein_id="HRZ75299.1"
                     /translation="MLSFIKRNTLLKVLSLNSISVIINFILGIISLKVISVYLGTSGM
                     ALTGSFRNFTTLFKSLSTNGFSQSLIKLFVENKEDKKESSVVISTFFWLFLVVSVLLG
                     ILIILFSEPISYFVLRNDEYAFYIKIFGLILPFVALQSFMTSVFNGLLWFKKIVFLQI
                     VSSVVVFIATVSLIYYHRLEGAFIAIALADFILFLIVLFVAFQNKAFLQFDLQYIIAK
                     KHFSVIRDFSIMALLSGIIVPLNFILIRNLIIDYHSIQQAGIWDAVNRISGFYMLFFT
                     TGLSFYYIPKLSSIHTNAEFKGELKNYFKTIIPLFIVVVLLFIFFKDIVIQIALTEEF
                     QEVKTVLFWQLFGDFLRLLTLAFGYQILVKTMMKKYIAVELLFNISYFVLSYFMIQKS
                     AVTGALQAYCIANFITFCLVFWMFRNTLFSKRN"
BASE COUNT         1699 a          923 c          899 g         1667 t
ORIGIN      
        1 catcactaat ttcaattaca attccggtgc cgttaccaat gtcgtccacc gcaaaaacta
       61 gttcaaatgg attcgttatc ccttccggaa gcaggagtgt aatgctgttt gattcgcttc
      121 cgtcaatggc aatcacattc atagtggcag cagaagcaat gacaacaccg ttaacataaa
      181 ccgcaatagg ggtgttggcc ggcaacggat tggtagcatt caaattataa acggtatagt
      241 caaccgtaac ttcattggaa ttacaactga ggtaaacgtt gtcaatcaca atcgtcgcat
      301 ccggaacctg actgtttaat ttagtcacca cggcattaat catcacaaaa tcctgacccg
      361 aggtcaacgc tatttccgct gattggtcac ccggctgaat gttattttga atatcataaa
      421 tatctaaatc catattaaac aaggccgaac ttcccgtgat gctattcgtg ccgttaaaag
      481 cattattctc cggattcaaa ggcggattac tcaaaatatt cccgttgatt tgcaacgttt
      541 cctcaaacgc tagattagaa tcaccttccc aagcaataaa acctattttt gctccgatat
      601 catcaatcac attcaagttg tctaaaacaa aagtcaaatt agtattgcca aaactcacat
      661 agtctaatcc gtcatacaca ttcaattgat tcagtggcaa cgaatcattt ttataaacaa
      721 taatcatggc ccaaccggca aagttggtag cattcgggca atagtccgga ataagctcag
      781 tcaaatcaag ttcagagagc gtataatcac catttccggt agaaagtact tgttccgtaa
      841 catctttaaa agcactaaag aagattaaac cggaagttga ttgaataaga ttaaaagttc
      901 ggtcaggcgt aatggaaatt ccgttgaatt gaacctcaaa atcacccggc cccgaacctg
      961 cccaatacag aaaagccttt tcaatcgcat catcaggttg catcgctaaa gtagccgtag
     1021 cagaggtgtt gatgatgcaa ggagacagaa tccctctgtt ttcttccgtg tttagcgtat
     1081 tgccaataaa cgtgaaatca tacctgccat taaactgttg atacaaacta acctcttgcc
     1141 cccaaacaga gaatgcaaaa aaatgtagta gccataacag aaaatagttg tattttcgtt
     1201 tcataaagta taaatcctcg atttgggatt taaagatact attttattga atataaaaaa
     1261 agcctcgaag cattcgagtg agtcatacgg tgcagcaata cctgataaga aaatatactt
     1321 cagctgatta ttcaatttgg aatgatttta tcaatcaagc caaaaatgct acttttttat
     1381 ttcaccgcga ctttatggaa taccatcatg atcgcttcga agactattca ctcatcgtag
     1441 aagatgaaaa aggatggttg gccgttttgc cggctaatcg agtaggagag gaagtctttt
     1501 cacaccaagg gttaacctat ggcggattgg tttttaaagg agatttaaaa ctagaaaaag
     1561 tactgctgat tttcaaatca attttacaac aacttcatat aagcgaaatt aaaacactac
     1621 atataaaaga attacctttt atctattctg attatttttc agatgaacta aagtatgctt
     1681 tgtttctcgc tgaagctaaa ttgtctcgcc gcgatacctt agccgtaatt gatttatcaa
     1741 aagaaattaa actggcatca ggcagaatgg aaggcgtaaa aagaggaata aagaacgaat
     1801 tgataattaa agaagaaaat gattttactt ctttttggaa cttggtgtta attccgaatt
     1861 taataaataa gcaccaagca aacccggttc attctttaga agaaatttct aatctgaaac
     1921 agaaattccc tttaaatatc cgacagttca acgtgtacaa agaaaatgaa atagtgggcg
     1981 gcaccaccat tttcgaaagt aaaaaagtag ctcattcaca atatatttca gtcacatcct
     2041 ctaagaatga atacggaacg ttagattttc tgcatcacta tttaatgaca gaagtattta
     2101 aagataaagc gttttttgat tttggtattt ccaatgaaga acaagggcga aaattaaacg
     2161 gcggtctttc cttttggaaa gaaagtttcg gagctaaaat tgtaacgcag gatttttatc
     2221 aagttcaaac agaaaattat atccaattgg agcaagtaat cgtatgataa aatttttcga
     2281 tttacataaa gtcaacaagc agtacgaaaa agctttccat gaaaagttag agcagttcat
     2341 gcaacgcggt tggtatattc tgggagacga agtacaatca tttgaaaacc aatttgccgc
     2401 tcattgcgga accaaacatt gcattggagt agggaatggt ttagacgctt tagtcttgat
     2461 tttcaaagct tacctcgaac ttggaaaatt acaaaagggc gatgaagtaa tcgttccggc
     2521 aaacacctat attgcgagca tcttatccat tttacaaaca gatttagtcc cggtgttagt
     2581 agaacccaag ttagaaacct tcaatttgaa tccggattta atagccgcta aaattacttc
     2641 caaaaccaaa gctattttgg tcgttcattt atacggacag ttagcggata tggaaaaaat
     2701 tgattcgatt gcaaagcaac acaacttgtt aatcatnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnaaaa atttaggttg tttaggcgat ggcggagcag tgcttacaaa
     2881 tgaggaggat ttgaacaagg tcattcgtgc tttgcgaaat tacgggtcag agaaaaaata
     2941 cgtcaatgac tacctcggaa taaattcccg cttagatgaa attcaagccg gctttctaaa
     3001 tgtaaaatta cccaacttag cgaatgataa tcaaaagcga agacaaatcg ccaatcgtta
     3061 tttagctgaa attaaaaacg ataaaattgt tttacctttt tatgatggtt caaccaatca
     3121 tgtgtttcat ttgtttgtca tccgcacaga aaagagaacc gaactacaag actatttaaa
     3181 agaaaacgga atcgaaacca tgattcatta cccgattccg ccacatcaac aaaaagcatt
     3241 gaagccattt aattcgttat ctttcccaat cacagaaaaa attcaccgtg aagtcttgag
     3301 tttacccatg agtccggtgc tcacagaaga ggaagtgagt cgtattattc aagttttaaa
     3361 tcaatattaa tggggttatt ctataaaata aagcaactac tgtttgttga ggtaagaatt
     3421 ttaaaattca aacttttatc cgattgtaaa aacacagttg gtaaaccaaa cctttatcat
     3481 ccgctcctgc tgaaaggaaa aggaagtatc tcttttggca ataatgttca aattggtgtc
     3541 gttgcctcac ccaattattt ttctcattat gcttatttag aagccagaga atcaaatagt
     3601 gcaattcaaa tcgggaataa tgtttccata aacaatgctt tttcagctgt tgccttttca
     3661 aaaatcttaa ttggagataa tgttttaatt ggcacaaatt gttccttgat ggacaatgat
     3721 gcacaccatt tagctagtaa caaacgccat gagtcctcca tttcaaaaga aatagtaata
     3781 gagaataatg tattcattgg ttccaatgtc actattttaa aaggagttac gattggtgaa
     3841 aatacggtga ttggaaatgg ttcggttgta acaaagtcta ttcctaaaaa tgtaattgcg
     3901 gctggaaatc cggctcatgt cattagagaa ttaccgtgct aagttttata aaaagaaata
     3961 cgctactcaa agtactttcc ctgaattcca tatcagtgat tatcaatttt atactgggca
     4021 tcatttcttt aaaagtaatt tccgtctacc ttggcacttc aggaatggct ttaaccggca
     4081 gtttccgaaa cttcacaaca ctttttaaat cattatcaac gaatggtttt tctcagtctt
     4141 taataaaatt atttgtcgaa aataaagaag ataaaaaaga gtcgtctgtg gtgatttcca
     4201 ctttcttttg gctcttcctt gttgtttctg ttcttttagg aatactaatc attttgtttt
     4261 ctgaaccaat tagttatttt gtgttgcgga atgatgagta tgctttctac attaaaattt
     4321 tcggattgat attgccattt gttgcgttgc aatcgtttat gacctctgtt tttaacggat
     4381 tgttgtggtt taagaaaatt gtttttctac aaattgtatc atctgttgtg gttttcatcg
     4441 caactgtttc attaatatat taccatcgcc ttgaaggtgc ttttattgcg attgccctag
     4501 ctgattttat acttttctta attgtattat ttgttgcttt tcaaaacaaa gcttttctgc
     4561 aatttgattt gcaatacatt atagccaaaa agcatttttc cgtaattcgt gatttttcaa
     4621 ttatggcttt gttaagcgga attattgtcc ctttaaattt tattttaata cgaaacctga
     4681 ttattgatta tcattccatt caacaagcag ggatttggga tgcagtaaac agaatatctg
     4741 gattttatat gctctttttc acgacaggac tttcattcta ttatatccct aagttatcgt
     4801 caattcacac aaatgctgag tttaaaggag aattaaaaaa ctatttcaaa acgattatac
     4861 cattattcat agttgtagtt ttgttgttca ttttttttaa agatatagtc attcaaatcg
     4921 cattgactga agaatttcaa gaagtaaaaa cggtgctgtt ttggcagttg tttggtgact
     4981 ttttaagact tttaaccctc gctttcggtt accagatttt agtaaaaaca atgatgaaaa
     5041 aatatattgc agtcgaactg ctttttaata tttcttattt tgttttgtcc tattttatga
     5101 tacaaaaatc agctgtaacg ggtgcattac aagcgtattg tattgccaat tttataacgt
     5161 tctgtttggt gttttggatg tttagaaata ctcttttttc taaaagaaat taattgctat
     5221 tccaagtttc agtatacttc tcggcaatct tcacattatc atgttccttt tcaacaaaag
     5281 cacgtgca
//
LOCUS       DAOTCW010000154         5285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_11778_length_5285_cov_41.937476, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000154 DAOTCW010000000
VERSION     DAOTCW010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11778_length_5285_cov_41.937476"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..772)
                     /gene="tpiA"
                     /locus_tag="P5335_10245"
     CDS             complement(20..772)
                     /gene="tpiA"
                     /locus_tag="P5335_10245"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRZ75300.1"
                     /translation="MRQKIVAGNWKMHKTAEETEDLLNELINKIPTDIEQRVIVAPTF
                     INLAAAADHLEFTNIEVAAQNMHQAESGAFTGEISASMLKSVGINIVILGHSERRAYF
                     HETDALLANKVDTALKHEMEIIFCFGEELKDRQSGQHFNVVENQLKDGLFHLEAAAWK
                     NIILAYEPVWAIGTGETASPDQAQEMHDFIRDTINKKYGNSVSEDVSILYGGSVKPEN
                     AAEIFSKPDVDGGLIGGAALKSDDFTKIVLAI"
     gene            complement(772..1272)
                     /locus_tag="P5335_10250"
     CDS             complement(772..1272)
                     /locus_tag="P5335_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRZ75301.1"
                     /translation="MKKILLTVCVALGIISCSNAQKTAFTKEALEDKMLSVDGVELSF
                     QTILKKYEGKTVIIDVWASWCPDCVKGMPKVVELQKQFPDAVYLFLSYDKTPESWSKG
                     IEKYGVKGEHYLILSKWKGGTFSNSIDLDWIPRYMVLNKKGEISLYKAIEADDEKLIS
                     TIKNLN"
     gene            complement(1354..2430)
                     /locus_tag="P5335_10255"
     CDS             complement(1354..2430)
                     /locus_tag="P5335_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRZ75302.1"
                     /translation="MIRYLFHKIGYALLTLFGVITVIFILFTILPGDPARMMLDQNEN
                     SEQLALVKKKYGFDKPISTQYLYYLNDLSPLSFHSTKSDSYTFLSEGKYNGINFITIG
                     NTNIVLKIPYLRESFQKSGKKVSDVIGNTLPNTFILAFFAIIIAIVIGVFLGIISALY
                     KDTLLDRLIALVSSLGMSIPSFFSAILFAWLFGFVWHSYTGLNMTGSLYEVDDFGEGS
                     HIQWKNIILPAIVLGIRPLGVVIQLMRNSLLETFGQDYIRTAKAKGLNSFQIIKNHAL
                     KNSLNPVVTAISGWFASMLAGAVFVEYIFGWNGLGKEIVEALNNLDLPVIMGSVIVIA
                     TTFVVINILVDLVYAYLDPKIRLS"
     gene            complement(2427..2630)
                     /locus_tag="P5335_10260"
     CDS             complement(2427..2630)
                     /locus_tag="P5335_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75303.1"
                     /translation="MKNTFTIGNSIASVFMIIIGVNLLFIHQKSDFLPKYVTIILGIL
                     ILFISIGGILARIKNLKKDTSKN"
     gene            complement(2632..3738)
                     /locus_tag="P5335_10265"
     CDS             complement(2632..3738)
                     /locus_tag="P5335_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRZ75304.1"
                     /translation="MKVLTHIVRFLVGLLFIISGLIKLNDPVGFSFKLEEYFSEGVLN
                     IPFLVPLALLIAVVVVIFEIVLGVMLLIGFKPKFTVWSLFLMIVFFTFLTFYSAYFNK
                     VTDCGCFGDALKLTPWESFTKDIVLLVLIVFLIINLKFIQPIVGKLGQNATVFMSYTA
                     CLFLAYYVLMHLPVIDFRAFAVGNNIQKGMEIPAGATLSEYEMLFIYKVNGVETEFSQ
                     ADVMANKVPEAAEFVDRKDKLIKQGYVPPIHDFTIELDGSDYTEDMLKEPKLIMLISY
                     DLAKADDDGLAKLEEFHQAALKKGYKVIGMTASDETLIKSVKDKHKLTFDYYFCDATT
                     LKTIERANPSIVVLNKGTIVQKKHFNDIDKVTLN"
     gene            complement(3743..4288)
                     /locus_tag="P5335_10270"
     CDS             complement(3743..4288)
                     /locus_tag="P5335_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HRZ75305.1"
                     /translation="MDSTSQQYDKVIAVCRQLYCNKMKDYGSAWRILRLPSLTDQIFI
                     KAQRIRSLQENEIRKVDEDETGEFIGIINYSIMALIQLEKGVVEQPDLNLEEATRLYD
                     EKIAETKSLMENKNHDYGEAWREMRVSSLTDLILQKLLRVKQIEDNKGKTLVSEGIDA
                     NFQDMINYAVFALIHLGFHKK"
     gene            4374..5198
                     /gene="folP"
                     /locus_tag="P5335_10275"
     CDS             4374..5198
                     /gene="folP"
                     /locus_tag="P5335_10275"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRZ75306.1"
                     /translation="MLINCQGKLIDLATPNVMGILNVTPNSFYDGGKYKNESEIVKQV
                     ETMLVDGADFIDIGAYSSKPNAEFVSEEEEIARLIPVLKLVLKEFPTTLLSVDTFRSE
                     VAKAAVKSGASIINDISAGSLDDKMMETIGKLQVPYIMMHMKGTPQTMTQLTQYDDIV
                     KEMLFYFSEKVALARSFGINDLIIDPGFGFAKTVEQNFEIMQKLELFKVLELPILAGV
                     SRKSMIYKPLDLTPEKALNGTTALNMIALTKGASILRVHDVKEAVECVKLFSLMSL"
BASE COUNT         1765 a          936 c          841 g         1743 t
ORIGIN      
        1 ttattttgta ctctaatttt tatattgcca aaacaatctt tgtaaaatca tcgcttttca
       61 aagcagcacc acctattaaa ccaccgtcta catcgggttt agagaaaatt tcggcagcat
      121 tttcaggctt aacgcttcct ccatacaaaa tggaaacatc ttcagaaact gaatttccgt
      181 attttttatt tatggtatct ctgataaaat catgcatttc ttgagcttga tcaggtgaag
      241 cggtttctcc cgttcctatg gcccaaacag gttcatacgc taaaataata tttttccagg
      301 cagcagcttc taagtgaaac aatccgtctt ttaattggtt ttccactaca ttaaaatgtt
      361 gtcccgattg acggtctttt aattcttcgc caaaacagaa aataatttcc atttcgtgtt
      421 tgagtgccgt atctacttta ttggctaaca aagcatcggt ttcatgaaaa tatgctctac
      481 gctctgaatg acctaaaata acaatgttta taccgacact ttttaacata gaagcggaaa
      541 tttcacctgt aaaagcacct gattcagcct gatgcatatt ttgtgccgca acttcaatgt
      601 ttgtaaattc taaatgatct gccgccgccg ccaaattaat aaacgtagga gcaacaatta
      661 ctctttgttc aatgtctgtt ggaattttgt tgattaattc atttaataaa tcttcagttt
      721 cttcagccgt tttgtgcatt ttccagtttc cggctacgat tttttgtctc attagtttag
      781 attttttata gtagaaataa gtttttcatc atccgcttca attgctttgt atagagaaat
      841 ctcccccttt ttattcaata ccatatacct tggaatccaa tctaaatcga tagaattact
      901 gaaagttcca cctttccatt tggataaaat taaataatgt tctcctttaa ctccatattt
      961 ttcaattcct tttgaccaac tttcaggagt tttatcatag gataaaaata aatagacagc
     1021 atcaggaaat tgtttttgta attcaactac tttaggcatt ccttttacgc aatcaggaca
     1081 ccaagatgcc caaacatcaa tgataaccgt tttaccttca tattttttta aaatagtttg
     1141 aaaagataat tcaacaccat caacagaaag cattttatct tctaaggctt cttttgtaaa
     1201 agctgttttt tgagcattgg aacacgatat tattcctaga gccacacata cggtaagcag
     1261 tatttttttc attttcattt tattaattaa acaaatttac aataactagg gaaacgaaat
     1321 cctaataaac tgctaataat atccgaaatc gatttagctt aatcgaattt ttggatctaa
     1381 ataggcataa accaaatcaa ccaaaatatt aataacgacg aatgtagtgg caatcacaat
     1441 tactgaaccc atgatgaccg gtaaatctaa attgtttaat gcttccacaa tttcttttcc
     1501 aagtccattc caaccgaaaa tatattccac aaaaactgcc ccggctaaca tggaagcgaa
     1561 ccatcctgaa atggcagtta caacagggtt caaggagttt ttcaaggcgt gatttttaat
     1621 gatttggaat gagtttaaac ctttagcttt tgccgttcga atataatctt gtccgaatgt
     1681 ttccaacaat gaattccgca ttaattgaat aacaacacct aaaggacgaa ttcctagaac
     1741 aattgccggt aggattatat ttttccattg aatgtgactt ccttccccaa aatcatctac
     1801 ttcatataaa cttccggtca tgttgagtcc ggtatagcta tgccaaacaa atccaaataa
     1861 ccaagcgaat aaaatggcac tgaaaaagga aggaatactc atgcctaaac tactcactaa
     1921 agcaatcaat ctatctaata aagtatcttt gtaaagagct gaaataatac ccaaaaaaac
     1981 gcctataaca atcgcaataa taatggcaaa aaatgctaaa atgaaagtat ttggtaaagt
     2041 gtttccaata acatcgctaa cttttttacc gcttttttga aaactttctc tcaaataggg
     2101 tattttcaaa acgatgttgg tatttccaat ggtaatgaaa ttgattccgt tgtatttccc
     2161 ttcagataaa aaagtatacg aatctgattt tgtactatga aaagacaaag gtgacaaatc
     2221 gtttaaataa taaagatatt gagtagaaat aggtttgtcg aaaccatatt ttttcttaac
     2281 caacgcgagt tgctctgaat tttcattttg atctagcatc attcgggcag gatctccggg
     2341 aagtatcgtg aataaaataa aaatgacggt gattactcca aacagggtga gcaatgcgta
     2401 gccaatttta tggaataaat aacgaatcaa tttttagaag tgtctttttt taaattcttt
     2461 attctggcaa gtattccacc tatagagatg aatagaataa gaatacctaa aataatcgtg
     2521 acatattttg gcagaaaatc acttttttga tgaataaata ataggtttac accaataata
     2581 atcataaaaa ctgaagcgat gctattgcct atggtaaagg tgtttttcat cttaattcaa
     2641 cgttactttg tcaatatcat tgaaatgttt tttctgaaca atcgttcctt tgtttaaaac
     2701 aacgatactc ggattagctc tttcaatggt ttttaaggtg gtagcatcgc aaaaataata
     2761 gtcaaacgtt aatttatgct tgtcttttac agacttgatt aatgtttcgt ctgaagctgt
     2821 cattccaatg actttatatc cttttttgag agcagcttga tgaaattctt ccagtttagc
     2881 taagccatca tcatccgctt ttgctaaatc atatgaaatc agcataatta atttaggttc
     2941 tttgagcatg tcttccgtat aatcacttcc gtctaattca atggtaaaat catgaatagg
     3001 aggaacgtaa ccctgtttga ttaatttgtc tttacgatcg acaaattctg ctgcttcagg
     3061 aactttgttg gccatcacat cggcttgaga aaactcagtt tccacgccat tcactttgta
     3121 aataaaaagc atttcatatt ccgataaggt cgcacctgct ggaatttcca ttcctttttg
     3181 gatgttattt ccaacagcaa aagcccgaaa atcaataacc ggtaaatgca tcagcacata
     3241 ataagctaaa aacaagcaag cggtatacga cataaaaacg gttgcatttt ggcctaattt
     3301 tccaacaatc ggttgaatga atttcagatt aattattaaa aacacaatta ataccaataa
     3361 gacgatatcc tttgtaaacg attcccatgg cgtaagtttc agtgcatcgc caaaacaacc
     3421 acaatcggtt actttattaa agtaggctga ataaaaggtt agaaatgtga aaaatacaat
     3481 cattaaaaaa aggctccaaa ctgtaaattt cggtttgaat ccaattaaaa gcattacccc
     3541 taagactatt tcaaaaatga ccacgaccac tgcaattaat aaggcaagcg gaactaaaaa
     3601 agggatgttt aaaactcctt cagaaaaata ttcttccagt ttaaatgaaa aacctaccgg
     3661 atcattcagt tttattaatc cggaaataat aaataataat ccaacaagaa aacggacaat
     3721 gtgtgtgagt actttcatga ttttattttt tgtgaaatcc taagtgaatt aaggcaaaaa
     3781 cagcataatt aatcatgtct tgaaaattag catcaattcc ttctgaaact aacgttttac
     3841 ctttattatc ttcgatttgt tttacacgta gcaatttttg caaaattaaa tcggtgagag
     3901 agctcacacg catttcacgc caagcctcgc catagtcatg gtttttattt tccatgagtg
     3961 attttgtttc tgcgattttt tcgtcatata agcgagttgc ctcttctaaa tttaaatccg
     4021 gttgctcaac gactcctttc tctaattgaa ttaaggccat gatagaataa ttgataatac
     4081 caataaattc accggtttca tcttcatcta ctttgcgaat ttcattttcc tgtagactgc
     4141 gaatgcgttg agctttgatg aaaatttggt cagttaggga gggcaaacga agaattctcc
     4201 atgcactgcc atagtctttc attttattgc aatataattg tcggcaaacc gcgatgactt
     4261 tatcatattg ttgtgaggta ctatccatta ttgaattata tttgtaaaga ttttcaaaaa
     4321 tacaattttc aaaagaagta gatgctactt tttaacgcct tttttcaaac gatatgttaa
     4381 taaactgcca aggcaaactg atagatttag cgactcctaa tgtgatgggg attttaaatg
     4441 taacaccaaa ttcattttat gacggaggaa aatataaaaa tgaatccgaa attgtaaaac
     4501 aagtggaaac catgttggtt gatggagctg attttattga tatcggagct tattccagta
     4561 aacccaatgc cgaatttgtc tccgaagaag aagaaatagc ccgtttaatt ccggttttaa
     4621 aattggtttt aaaagaattt ccaacaactt tattatccgt tgataccttt agaagtgaag
     4681 tggctaaagc tgcggtaaaa tcgggtgcca gtatcattaa cgatatttcg gcaggaagtt
     4741 tggatgataa aatgatggaa acgataggaa aacttcaagt tccatacatt atgatgcaca
     4801 tgaagggaac accacaaacg atgactcaac tcacacaata tgatgacatt gtaaaagaaa
     4861 tgttgtttta tttttctgaa aaagtggctt tggcaagaag ttttggcata aatgatttaa
     4921 ttattgatcc cggatttggt tttgctaaaa cagtagaaca aaattttgaa attatgcaaa
     4981 aattagaatt atttaaagtg ctagaattgc caattttagc cggagtttcc agaaagtcaa
     5041 tgatttataa gccattggat ttaaccccgg aaaaagcgtt gaacggcaca acagctctca
     5101 atatgattgc cttaaccaaa ggagcttcta ttttgcgggt tcatgatgtg aaagaagcgg
     5161 tggaatgtgt aaaattattt tccttaatga gtttataata tgaagaaagt tttagtacta
     5221 ttcgttttac tctcttttgt ttcctgtcaa aaagaagtgc aattagttca ggcagtcagc
     5281 actat
//
LOCUS       DAOTCW010000155         5282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_11792_length_5282_cov_29.341305, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000155 DAOTCW010000000
VERSION     DAOTCW010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11792_length_5282_cov_29.341305"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="P5335_10280"
     CDS             <1..1383
                     /locus_tag="P5335_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HRZ75307.1"
                     /translation="SKKEERVIAFMMDFGKQLGLETIKDEVGNVIIKKPATAGLENRK
                     TIVMQSHLDMVHQKNNDTNFDFDTQGIEMFVDGDWVRAKGTTLGADNGLGVATIMAIL
                     ESKDIPHPDIEALFTIDEETGMTGAIGLKGELLNGEILLNLDTEEDDEIDIGCAGGVD
                     VTATKSYKEEETPENSLAFTITVKGLKGGHSGMDIHKGLGNANKIMNRLLFDGFENFG
                     LQIVEINGGSLRNAIPRESVAKVIISAIYEGAFQLDMQEVIDAIKTEYKTTEPNLTIE
                     ITETELPKKVMDLGVQEGLLRAIYAAHNGVYRMSADMEDLVETSNNVAKVIVKEGEIT
                     VQCLTRSSVETSKFDLANALRSAFELMGCEVTFGGSYPGWTPNVKSPILDVLVSIYEK
                     QNGKKPNVVACHAGLECGILGTNYPDMDMISFGPTIHGAHSPDERASISSAQKYWKFV
                     LEILENIPVR"
     gene            complement(1429..1908)
                     /locus_tag="P5335_10285"
     CDS             complement(1429..1908)
                     /locus_tag="P5335_10285"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HRZ75308.1"
                     /translation="MKNLSVLVLILLMCLFSCQQKTIFSELKKTNWFLGRWENKTPEG
                     TFSEEWKIENDSLLLGKSFFIKENDTLFSETVRLVQREKDLFYIVTVPNQNEAKPVEF
                     KLTSSTADYLVFENPEHVFPKKITYKLVNKDSLYAEISGDGKSQGFPFKKVNRKFAN"
     gene            2015..3088
                     /gene="prfA"
                     /locus_tag="P5335_10290"
     CDS             2015..3088
                     /gene="prfA"
                     /locus_tag="P5335_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRZ75309.1"
                     /translation="MLDRLQYVKQRFDEISDLIIQPDVIADQKRYVQLNQEYKTLKGL
                     VDKREEYIRLTQNIEEANEIIADGSDAEMVEMAKMELDEAKDRLPQLEDEIKFMLIPK
                     DPEDAKNVMVEIRAGTGGDEASIFAGDLYRMYTKYCEGRGWRTSLVDMNEGTAGGFKE
                     VIFEVTGEDVYGTLKFEAGVHRVQRVPQTETQGRVHTSAATVMVLPEAEEFDVQIDMN
                     DVRIDYFCSSGPGGQSVNTTKSAVRMTHVPTGLVAQCQDEKSQHKNKDKALTVLRSRL
                     YEMELAKKQAEDATKRTSQVSSGDRSAKIRTYNYAQGRITDHRIGLSIFDLDGFMNGN
                     IHKMIEELQLVSNTEKLKESEVF"
     gene            complement(3154..4152)
                     /locus_tag="P5335_10295"
     CDS             complement(3154..4152)
                     /locus_tag="P5335_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRZ75310.1"
                     /translation="MKNLRKYIQLFYEVIKDTFDGFIEFKVLKMSASLAYITVFSLAP
                     LLLVILFICDVFWGREAIEGAIYSQVNSFVGTSSAIQIQAMIQNLALSKNSTVAAVIG
                     IGTLLFGATSVFAEIQDSINTIWGLRHKKKSGFWLFIKARLLSFGVIGSLGFILLVAL
                     GFSTLLDGISNQLTQTYTEISIVVFYVSNLIATLIITSLLFGAIFTILPDADITWKQV
                     RVAAFTTTILFILGKFLITFYIANSNISDVYGAAGSIVVLMVWVYYSSVILYLGATFA
                     KCYAIKFAEPIKPSKYAEIVQYITVTTDAKTIQSADDEIKELKKEPDISIEDIKPS"
     gene            complement(4136..5257)
                     /locus_tag="P5335_10300"
     CDS             complement(4136..5257)
                     /locus_tag="P5335_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRZ75311.1"
                     /translation="MVVTPNKENSNALNLLFIIGLVFCLYILKPIIVPLIMALILTVS
                     VFPLVNFLEKKFHVNRVFSALTAIFTLSIVLFGLLAFVVFQLTDILEKSDQYILRLTE
                     VYNTILNYFDTNFGIRKSELSLKKLNIGAAIKDNFTSIIDFLTSSGSVFSDIVLIPLY
                     MFFFLIYRKFFKSFLFKAFSVDGNILRVKSVLAKLYKVQQNYLYGLFTVMFIVGLLNT
                     LGLLILGIENALFFGFLAAILLLIPYIGIMIGSLLPALVALATKDSAWYAVGVIAVFM
                     LIQMIEGNYITPKITGSKVSINSFVAILSIILFSMLWGTAGMIVALPVIASLKVVFDS
                     IPELKPYGFLIGEPLEEHLRSDARIRLKNWKKIKNEKST"
BASE COUNT         1862 a          836 c         1021 g         1563 t
ORIGIN      
        1 tcaaaaaaag aggaacgtgt cattgctttt atgatggatt tcggaaagca attaggcttg
       61 gaaaccatca aagatgaagt aggaaacgtc atcatcaaaa aaccggctac tgcaggatta
      121 gaaaacagaa aaaccatcgt gatgcaatcg catttagaca tggtccatca aaaaaataac
      181 gataccaatt ttgattttga tacacaagga atcgaaatgt ttgtggatgg tgattgggtt
      241 cgtgccaaag gaacgacact tggagccgac aacggtttgg gtgttgcgac tataatggcg
      301 attttggaaa gtaaagatat tccgcatccc gacattgaag cactttttac cattgacgaa
      361 gaaaccggaa tgaccggagc cataggttta aaaggcgaac ttttaaatgg agaaatctta
      421 ttgaatttag acaccgaaga agacgatgaa attgatatcg gttgtgccgg tggtgtagat
      481 gttaccgcaa ccaaaagtta taaggaagaa gaaacgcctg aaaactcgtt ggcttttaca
      541 attaccgtaa aaggtttgaa aggcggacat tcaggaatgg atattcataa aggattaggg
      601 aatgccaata aaattatgaa ccgtttattg tttgacggtt ttgaaaattt tggtttgcaa
      661 attgttgaaa tcaatggagg tagtttacga aatgccattc cgagagaaag tgtagccaaa
      721 gtaataattt ctgccattta tgaaggagct tttcaattgg atatgcaaga agtgattgat
      781 gctatcaaaa ccgaatataa aactaccgaa cctaatttaa ccattgaaat tacggaaacc
      841 gaattgccaa aaaaagtaat ggatttaggt gttcaagaag gtttacttcg tgcgatttat
      901 gcagctcata acggcgttta ccgaatgagt gccgatatgg aagatttagt tgaaacttca
      961 aataatgttg cgaaggttat agttaaagaa ggagaaataa ctgttcaatg tttaacccgt
     1021 tcttcggtag aaacatcaaa atttgattta gcgaatgcct tgcgttctgc gtttgaattg
     1081 atgggatgcg aagtaacatt tggtggaagt tatccgggtt ggacaccaaa tgtgaaatca
     1141 ccaatcttag atgttttggt ttcaatttat gaaaaacaaa atggtaaaaa gcctaatgta
     1201 gtagcgtgtc atgccggttt agaatgtgga attttaggaa ccaattaccc tgatatggat
     1261 atgatttctt ttggtccaac gattcacgga gctcactctc ctgatgaaag agcttcgatt
     1321 tcatcggcac aaaaatattg gaagtttgta ttggaaattt tggagaatat tcctgtaagg
     1381 taattttgat tatttattta ccgcaaattc gcaaattata actcattatt aatttgcgaa
     1441 tttgcggtta actttcttaa acggaaatcc ctgactcttt ccatcaccac taatttctgc
     1501 atacaaactg tctttgttta ctaatttgta agtgattttt tttgggaaaa catgttcagg
     1561 gttttcaaag actaaataat cagcagtaga agaggttaat ttgaattcca ccggtttagc
     1621 ctcattttga ttgggaaccg taacaatgta aaataaatct ttttcacgtt gaactaatcg
     1681 aacagtttcg gaaaaaagtg tgtcattttc tttgatgaaa aaacttttac ctaataacaa
     1741 agaatcgttt tcaattttcc attcttccga aaaagtgcct tccggggttt tgttttccca
     1801 acgtcctaaa aaccagttgg tttttttaag ttcactgaaa atggtctttt gctggcagga
     1861 aaaaaggcac ataagaagta taagtacaag tacgcttaag tttttcatga tggatgattt
     1921 aataaacaat actacaaaaa aacaatgaaa tgttaaactt gaatcttgaa acaaaaaatc
     1981 gtacttttgc actccaaaat acagttataa gactatgtta gacagattac aatatgtaaa
     2041 gcaacgtttc gacgagatat cagatttgat tattcagcct gatgtgattg ccgaccagaa
     2101 gcgttatgtg caattgaacc aagaatataa aacgttgaaa ggtttggttg ataagcgtga
     2161 ggaatacatc cgcttgaccc aaaatataga agaagctaac gaaataattg ccgatggttc
     2221 ggatgcggag atggttgaaa tggcaaagat ggaattggac gaagcgaaag accgtttgcc
     2281 acaattggaa gacgaaatca aatttatgtt gattccgaaa gacccggaag atgctaaaaa
     2341 cgttatggtg gagattcgtg ccggaacggg tggggatgaa gcatcgattt ttgcaggtga
     2401 tttatacaga atgtacacta aatactgcga aggtcgtggt tggagaacat ctttagttga
     2461 catgaacgaa ggaacagccg gaggttttaa agaggtaatt tttgaagtta caggagaaga
     2521 tgtttacggt acattgaaat ttgaagcagg cgttcaccga gttcaacgag ttccgcagac
     2581 tgaaacccaa ggacgagtgc atacatcagc ggcaactgtt atggttttac cggaagcaga
     2641 agaatttgat gttcaaattg atatgaacga tgtgcgaatt gattatttct gttcgtcagg
     2701 accgggagga caatcggtga atacgacaaa atcagctgtt cgtatgacgc acgttcccac
     2761 cggattggtg gctcaatgtc aggatgaaaa atcacaacat aaaaataaag acaaagcatt
     2821 aacggtttta cgttctcgtt tgtatgaaat ggagttagcc aaaaaacaag cggaagatgc
     2881 taccaaacga acttcgcaag taagtagtgg cgaccgttca gctaaaatcc gtacttataa
     2941 ttatgctcaa ggtcgaatta ccgatcaccg cattggtttg agtatttttg acttggatgg
     3001 attcatgaat ggaaatatcc ataaaatgat tgaagagttg cagttagtga gcaacaccga
     3061 aaaattaaaa gaatcagaag tgttttaaaa taggaaagcc aactaaattt agtcggcttt
     3121 ttcatttaga gatgatttta tccttattgg tttttagctg ggtttaatat cttcaattga
     3181 aatatcgggc tcttttttga gttctttgat ttcgtcatct gctgattgta tggtttttgc
     3241 atctgtggta accgtaatat attgaacaat ttctgcatat tttgaaggtt tgatgggctc
     3301 ggcaaatttt atagcataac attttgcaaa agtagctccc aaatataaaa taacagagga
     3361 ataatatacc caaaccatta aaacaacaat agaccctgca gcaccgtaaa catcgcttat
     3421 atttgaattt gcgatgtaga aagtaatcaa aaattttcct agtataaata aaatggttgt
     3481 ggtgaatgcg gcaacgcgaa cctgtttcca agtgatgtca gcatcaggga gtatggtgaa
     3541 aatggctcca aacagaaggg atgtgatgat taaagttgca attaaattgg atacataaaa
     3601 aaccacgata cttatttcag tataggtttg tgttaactga ttgctaattc catctaacaa
     3661 agtagaaaac ccgagtgcaa ctaaaagtat aaagcccaaa ctcccaataa ctccaaaaga
     3721 taataatcgg gcttttataa acagccagaa tcccgatttc tttttatgtc gtaatcccca
     3781 aatagtgttg attgaatctt gaatttctgc aaataccgat gtcgctccaa aaagcaaagt
     3841 tccaatacct ataaccgctg caactgtaga gtttttagat aaagctaaat tttgaatcat
     3901 agcttgaatt tgaatggcac ttgaagtccc aacaaaacta tttacttgtg aataaatggc
     3961 tccttcaatg gcttctctac cccaaaaaac atcacaaata aatagaataa caagtaataa
     4021 tggtgctagt gaaaaaacgg tgatgtaagc taatgaagca ctcattttaa gcactttaaa
     4081 ttctataaag ccatcaaaag tatctttaat aacttcataa aataattgga tatacttacg
     4141 tagatttttc atttttgatt tttttccaat ttttcaatcg gattctggcg tcgcttcgta
     4201 aatgttcttc taaaggttca ccaattaaaa agccataggg tttaagttcg ggaatgctat
     4261 caaaaacaac ttttaaactg gcaattaccg gaagggcaac aatcatgccg gctgttcccc
     4321 aaagcatgga aaaaaggatg attgaaagaa tcgcgacaaa tgaattaata ctcacttttg
     4381 atcctgtgat tttaggcgtg atataatttc cttcaatcat ttgtatcaac ataaaaacag
     4441 caataactcc tactgcatac caagccgaat cttttgtggc taaagcaaca agtgccggta
     4501 ataatgagcc aatcataatt ccgatatagg gtatcaataa aagtatggct gccaaaaagc
     4561 caaagaagag agcattttca ataccgagaa taagtaaacc aagtgtgttt aataatccaa
     4621 caataaacat tactgtaaat aatccgtaca aataattttg ttggacttta tataatttag
     4681 ccaaaactga ttttacgcgt aatatatttc catcaactga aaaagctttg aaaagaaaac
     4741 ttttaaagaa cttgcgataa atcaaaaaga aaaacatgta cagaggaatt aaaacgatat
     4801 ctgaaaaaac gctaccggat gaagttagaa aatcaattat gctcgtaaaa ttatctttaa
     4861 ttgccgctcc gatatttaat tttttcagag ataattcact ttttcggatg ccgaaattag
     4921 tgtcaaagta atttaaaatt gtgttgtaaa cttctgttaa tctcaggatg tattgatcac
     4981 ttttttcaag aatatctgtt aattgaaaaa cgacaaaagc taacaaacca aacaatacaa
     5041 ttgaaagagt aaaaattgcc gtaagagctg aaaaaacacg atttacatga aatttttttt
     5101 ctaagaaatt aaccaatgga aaaacagaaa ccgtaagaat taacgccatt atcaatggaa
     5161 caataattgg ttttagaatg tataaacaaa aaaccaaacc tattataaac aacaggttca
     5221 atgcattaga attttctttg ttaggagtta caaccatatt tgtctataaa ttattttaaa
     5281 aa
//
LOCUS       DAOTCW010000156         5151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_12248_length_5151_cov_30.166405, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000156 DAOTCW010000000
VERSION     DAOTCW010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12248_length_5151_cov_30.166405"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..541
                     /locus_tag="P5335_10305"
     CDS             164..541
                     /locus_tag="P5335_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75312.1"
                     /translation="MSEKSKKQFGVWMDSHHATIIGKEDFEEGAFLVLGHVNNAGPES
                     HSNEKTTNNQEIRLTQKFFKEIASLMTNVDEIHLTGTGQAQEQFMKFLADTPQYKNAV
                     SSESTSNKMSDENLILYVERYFN"
     regulatory      821..886
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            917..2227
                     /locus_tag="P5335_10310"
     CDS             917..2227
                     /locus_tag="P5335_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HRZ75313.1"
                     /translation="MTNRKQKKSATQKIKVQFKLFFRAYPALPYIIKWFLISSIIGVC
                     VGSASAGFLVSLDWATNFRESHLWLIALLPIGGLLIGLLYNYLGKDIEAGNNLLIDSI
                     HNPRERIPFRMAPFVFLGTIATHFFGGSAGREGTAIQMAGAIADQFTKPFKLNPSERK
                     ILLISAIAAGFGSIFGTPLAGALFGLEVFLIGKIKYNAIFPAFASAIIADLVTDLWQV
                     EHTHYHIDFIPQIGTLPILYSILAGIAFGLCAASFSKIIHYVTAIFKRKVSYAPLRPF
                     IGGIIVALAVLAIGTTKYIGLGIPTIVESFEQQVPIYDFALKMAFTIVTLSAGFKGGE
                     VTPLFFIGATLGSALSLFIPLPTGLLAGMGFVAVFAGATNTPLACIFMAVELFGVECS
                     IYVAIACVVSYLLSGHNSIYNKQVIGEAKHSNFESHSGKNINQL"
     gene            2232..2357
                     /locus_tag="P5335_10315"
     CDS             2232..2357
                     /locus_tag="P5335_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75314.1"
                     /translation="MKLTENHQRGINAIAVVLEKSKFDRIKVFKIKKHKYQKNEK"
     gene            2347..2889
                     /locus_tag="P5335_10320"
     CDS             2347..2889
                     /locus_tag="P5335_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRZ75315.1"
                     /translation="MKNKFNAWHDVSPGDNLPEIVNGIIEIPKGTRAKYELDKESGLL
                     ILDRVLYSSVYYPANYGFIPQSYCDDKDPLDILILSQIDIVPMCIVPAKVIGVMRMLD
                     NGEADDKIIAVAAGDPSVSHINDISELPLHFISEMRSFFEDYKKLEKKTVVVEDFLDR
                     ETAIQILQDSFKMYQEKFNV"
     gene            3082..3522
                     /locus_tag="P5335_10325"
     CDS             3082..3522
                     /locus_tag="P5335_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRZ75316.1"
                     /translation="MENNQDNSKIIRSKYVPTSEFYSQIIDSLQDYSIFTLDREFIIN
                     SWSSGSTKIFGYETDEVIGKSFDVIFTEDDLKNNIPKKEIEIALKEGRATDNRWHIAK
                     DKSLFYAYGLVFPLIGLEGELLGYVKILRDLTERKQSEDALRIM"
     gene            3519..4277
                     /locus_tag="P5335_10330"
     CDS             3519..4277
                     /locus_tag="P5335_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRZ75317.1"
                     /translation="MKELEELNTHKESVMAILSHDLRSPLSAIIGTAKYLKENFHKMK
                     PEAVQQMLDLLYKSSIDELEMLDYLVEWARIKYASDVFFPAQINLIDYIEKVFATLNE
                     TASVNTINLHHEIEADTSVFADSKMLISIIQNIVSNAIKHTEAGGEIKVTAKTQDDKI
                     IVRVKDNGIGMSTEIMEKLFTPQMKTLSETRKMNKGAGIGLLLVKGFLEKNGGEIWVE
                     SIEGEGSSFYFTLPIEKPLYKIGSSDEIMFDESP"
     gene            4377..>5151
                     /locus_tag="P5335_10335"
     CDS             4377..>5151
                     /locus_tag="P5335_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HRZ75318.1"
                     /translation="MLTLGIYHTLRIDRTTLVGLFLTDEKDDVLLPIKYVPKKFEMND
                     MLEVFVYLDHEERPVATTLKPYITLGDYAFLRVNYTNEYGAFLDWGLEKDLFVPFQEQ
                     ARPMEQGKRYMVHMYLDEKTNRLVGSSKINQFLSKEPLSLESGTEVDLIISHITDLGI
                     NVIVNGTHKGLVYKNEVYTDSIRPGDKTKGYIKIIRPDGKIDVTFNRIGVEAIEENAQ
                     VILDELKASRGFLRLNDNSSPDDIKTILKMSKKTFKKAVG"
BASE COUNT         1725 a          723 c         1037 g         1666 t
ORIGIN      
        1 tatttccgtt gccattggaa ttatgcttat aaaggctgta atttaaggtg attataaggt
       61 gctgaatgct atgttcgagg caaagcttta acgcactcac aatgttaacc caaaaaaaga
      121 agtttccttt ttgaaaggct tacctttata aaaagaaact actatgtcag agaaaagtaa
      181 aaagcagttt ggtgtttgga tggattccca ccatgctaca atcattggga aagaagattt
      241 tgaagaaggt gcatttttag tcttaggaca cgtgaataat gcaggaccgg aaagtcattc
      301 gaacgagaaa acgaccaata atcaagagat tagattaaca cagaaatttt ttaaggaaat
      361 cgcgagttta atgactaatg ttgatgaaat tcacctcacg ggaacgggtc aggctcaaga
      421 gcagttcatg aagtttttag ccgacacacc gcaatataaa aatgctgttt cgagtgaaag
      481 cacttcaaac aaaatgagtg atgagaattt gatactttat gtagaaaggt attttaatta
      541 gatttatttt ttttattttt atgagaaacc gttgaattat tcaacggttt tttgttagtt
      601 atataattgc attgagttga tgaaattata tttttttttt aaaatttaat tcaatttcta
      661 gaagagataa ttttttaaaa ttattgataa tcaatttttt aatgaatgat agctaaggat
      721 tctaactaat ttttcaacgt gtttaaataa aaacacaaag ttttataaat ataaaaaaac
      781 acttaaaaat ttgttcttac aaaattaaag tttaatttta cactatatag gaaatggtgt
      841 tcttcctaac ccaaccgccc aaaaagctga tgacgcctga ttgtagtaat gttaaatgca
      901 ttaaatcagg ataaatatga ccaatcgtaa acaaaagaaa tcagccactc aaaaaattaa
      961 agttcaattc aagttatttt ttagagcata cccagctctt ccatacatta ttaaatggtt
     1021 tttaataagt tccatcatag gggtttgtgt aggttcagct tctgcaggtt ttttagtatc
     1081 acttgattgg gcaacaaatt tcagagaaag tcatttgtgg ctcattgcac tattgccaat
     1141 aggcggatta cttataggtt tactttataa ttatttaggc aaagatattg aggcaggaaa
     1201 taatttatta attgacagca ttcataatcc aagagaaaga ataccgttta gaatggctcc
     1261 gtttgtcttt cttggaacaa ttgccacaca tttttttggt ggttcagcag gacgggaagg
     1321 cactgcaata caaatggcag gagccatcgc cgatcagttt actaaacctt ttaaactgaa
     1381 tccaagtgaa cgtaaaatat tattgatttc agctattgct gcaggttttg gttccatatt
     1441 tggaacacca ttggcaggag cattatttgg tttagaagtt ttcttaatcg gaaaaattaa
     1501 atataatgct atctttccgg cttttgcatc cgctattatt gctgatttag taactgactt
     1561 gtggcaagtt gagcacactc attatcatat tgattttatt ccgcaaatag gaacacttcc
     1621 catattatac agtattttag caggaatagc ttttggcttg tgtgcggctt cgtttagtaa
     1681 aattatacat tatgttactg caatttttaa aagaaaagtt agttatgcac cccttcgacc
     1741 atttattggc ggtattatcg ttgcattagc cgttttagcc attggcacga ctaaatatat
     1801 aggattggga attccaacca tagttgaatc gtttgagcaa caagttccaa tttatgactt
     1861 tgcactaaaa atggcattta ccattgtcac cctatctgcc ggttttaaag gtggagaagt
     1921 tacacctttg ttttttatcg gagcaacttt aggaagtgca ttgtcgttat ttattccttt
     1981 accaacagga cttttggcag gaatgggctt tgtagctgtg tttgcaggag ctactaatac
     2041 tcctttggct tgtatattta tggctgtgga actctttgga gtagaatgta gtatttatgt
     2101 agctattgcc tgtgtggttt cttacctgct ttcaggtcat aatagtattt ataacaaaca
     2161 agttattggt gaggctaaac attcaaattt tgaaagtcat tccggaaaaa atattaatca
     2221 attataagaa aatgaaactg accgaaaatc atcaacgagg aataaatgcc attgctgttg
     2281 ttttagaaaa atcaaagttt gacagaatta aagtattcaa aataaaaaaa cataaatatc
     2341 aaaaaaatga aaaataaatt taatgcttgg catgatgttt caccgggaga taatttaccg
     2401 gaaattgtaa acggaatcat tgaaattcca aaagggacga gagcaaaata tgaactagac
     2461 aaggaaagcg ggctattaat tttggaccga gttctttatt catcggttta ttatcctgcg
     2521 aattatggtt ttataccgca gagttactgc gatgataagg atccattgga tattttaatt
     2581 ttatctcaga ttgacattgt tcctatgtgc attgttcctg caaaagtaat tggggtaatg
     2641 cgaatgctgg acaacgggga agcggatgac aagataatcg cggttgcggc cggagaccca
     2701 agtgtgagtc atattaatga tatatcagaa ttgccgctgc attttatttc tgaaatgcgt
     2761 agtttttttg aggattataa aaagttagag aaaaaaacgg tggtggttga ggattttttg
     2821 gacagggaaa cggcgattca aattcttcag gatagtttta aaatgtatca ggagaagttt
     2881 aacgtataag aatgttttag aactataggt gtttttgtta atgattatat aaatggtttt
     2941 attgtgcttt aaaaccgata catttactaa ttaatcaata aaaatcagtt tttggtatag
     3001 tttttagtgg gagttgcgtt acgagcatta acggaaaaca cacttctaca atcattctat
     3061 ttgacaataa aaatatattc aatggaaaac aatcaagata attcaaaaat cattaggagt
     3121 aaatatgttc ctacctctga gttttacagt caaattattg acagtttaca ggattattcc
     3181 atttttacat tagaccgaga atttataatt aacagttgga gttccggttc aactaagatt
     3241 ttcgggtatg aaactgatga ggtgattggc aagtcttttg atgtaatatt tacggaggat
     3301 gatttgaaaa ataatattcc caaaaaagaa attgaaattg ctttaaaaga aggcagggcc
     3361 accgacaacc gatggcatat tgcaaaagac aagagtttgt tttatgccta tggtttagtt
     3421 tttccgttga taggattaga aggagagctg ttgggctatg tgaaaatatt gcgtgatttg
     3481 accgagcgga aacaatcgga agatgcgtta agaattatgt gaaagaatta gaagagctga
     3541 atacgcacaa ggagagtgtg atggcgatac tatcgcatga tttgcgaagt ccgttgtctg
     3601 ccattattgg aacagcaaaa tacttaaaag agaattttca taaaatgaag cctgaagctg
     3661 ttcagcaaat gcttgatttg ctttataagt cttcaattga tgaattagag atgttggatt
     3721 atttggtgga atgggcgagg attaagtatg cttcggatgt gttttttccg gctcaaatca
     3781 atttgattga ctacattgaa aaagtgtttg ctacgttgaa tgaaacggct tctgttaata
     3841 caatcaatct tcatcatgaa attgaagcag acacatcggt atttgcagac agtaaaatgt
     3901 tgatttcgat tattcaaaat attgtttcga atgcaatcaa gcacacagaa gcaggcggtg
     3961 aaataaaagt tacagcaaaa actcaggatg ataaaattat tgttcgggta aaggataacg
     4021 gaatcgggat gtcgactgaa atcatggaaa agctttttac accccaaatg aaaacccttt
     4081 cggaaaccag aaaaatgaat aaaggagcgg gaatcggttt gttgttggtg aaaggattct
     4141 tagaaaaaaa tggtggtgag atttgggtgg aaagtattga aggtgaagga tcttcgtttt
     4201 attttacgtt gcctattgag aaaccattat ataaaatagg cagttcagac gaaattatgt
     4261 ttgatgaaag tccgtaatct aaattagttt agcaaaaatt acttccacgt aaataccact
     4321 taaaatctac ttccggaaga ggtaaacaat tcataccttt gtggaaatta tacattatgt
     4381 tgactttagg tatttatcat acattacgca tagacagaac gactttggtt ggtttatttt
     4441 taactgatga aaaggatgat gttttgttgc ccattaaata tgttccgaag aagtttgaaa
     4501 tgaatgacat gctagaggta tttgtttatc ttgatcatga agaacgtccg gttgctacga
     4561 ctttgaaacc ttatatcacg ttaggagatt atgctttttt gcgggtgaat tataccaatg
     4621 aatatggagc ctttttagat tggggattag agaaagatct ttttgttcct tttcaagagc
     4681 aagctcgccc gatggaacaa gggaaacgat acatggttca tatgtatctg gacgaaaaaa
     4741 caaaccgatt ggttggttcc agtaagatta accaattttt gagtaaagaa cctttgtctc
     4801 ttgaaagcgg gacggaagta gatttgatta tatcgcatat taccgattta ggaatcaatg
     4861 ttattgtgaa cggaacgcac aaagggcttg tttataaaaa tgaggtgtat actgattcta
     4921 tacgtcccgg cgataagaca aagggttata ttaaaattat tcgccctgat ggaaaaatag
     4981 atgttacttt taatcgaatt ggtgtggaag cgatagaaga aaacgcacaa gttatattgg
     5041 atgaacttaa agcgagtcgc ggatttcttc ggttgaacga taacagttcg ccggacgata
     5101 taaaaaccat tttgaaaatg agtaaaaaaa cattcaaaaa agcagtagga a
//
LOCUS       DAOTCW010000157         5068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_12542_length_5068_cov_33.106324, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000157 DAOTCW010000000
VERSION     DAOTCW010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12542_length_5068_cov_33.106324"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1449)
                     /locus_tag="P5335_10340"
     CDS             complement(70..1449)
                     /locus_tag="P5335_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRZ75319.1"
                     /translation="MKKTLFLGIFALLAINAFSQGSLDYGGGLKFNLNPEGTKYMRFI
                     AWNQIWLTSTDLNPGTMIGDEPQSTFEDIGLRRLRLLAYAQISTRYMIVTHIGINNQT
                     FLNGGASGSSGTGGYGAGKKAGLFFHDAWNEYAVFLPKEDKAFSLSIGAGLHYYMGLS
                     RMTMSSTLNYLAVDAPIFNWPLIENSDQFARQIGLFAKGKYNKFEYRLSLNKPFATNQ
                     TPIDVNNEAIAKAVDNNNSSQWSKAGYFEYQIFDKESNFLPFKVGTYLGTKKMLNIGA
                     GFYNAPKATQTSVNGVIENHSINLLSADVFVDMPVGPKEKKMAVTGYSVFYNYDFGPN
                     YLRNIGIMNQGTVDPTFTGDYALAGPGNLQPTIGTGNIWYTQAGFLLPSKAEKPKVRI
                     QPFAAYTYKNFDALDQASNQFDFGSNFFLDGHHAKITAQYSNRPIYITPDTIDSYKGQ
                     FIVQLQIYL"
     gene            complement(1558..1977)
                     /locus_tag="P5335_10345"
     CDS             complement(1558..1977)
                     /locus_tag="P5335_10345"
                     /inference="COORDINATES: protein motif:HMM:NF017834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRZ75320.1"
                     /translation="MELLACPKCQSNQIVKSGVIKDRQRYLCKKCNYYFTVNKLGKKI
                     DDYYVTKALQLFLEGLSYREIERIIGVSHVTVSNWVKEYKIKKPAHTDYHPTYKIYNH
                     LELVEFLKNKEHLSGAGMIITELGDKFMLIKWERFKD"
     gene            2234..2839
                     /locus_tag="P5335_10350"
     CDS             2234..2839
                     /locus_tag="P5335_10350"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRZ75321.1"
                     /translation="MQFESNKTDPSVLYKLLTGIIIPRPIGWISTIDSNGINNLAPFS
                     YFNMVSSDPPCVMFSTRRENNKNKDTLNNVLQNGQFVVNLVTLEVVEQMNASSEAFPA
                     DVDEFIITNLTPIDSVSIQPKRVKESPIHLECEMIHHYFMDNNEGGGACIVIGKIKTI
                     HIDDSILMENNRINLDVYKPVARLAGSNYSTLGELFSIKRA"
     assembly_gap    2987..3086
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3087..>5068
                     /locus_tag="P5335_10355"
     CDS             <3087..>5068
                     /locus_tag="P5335_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRZ75322.1"
                     /translation="GPGGLYFSILTKKAMPHCQIDVYERNKPEDSFGFGVVFSDETLG
                     EFLMRDLKSYELIRSEFAYWDDIIVARDGQSVSISGNGFCGCSRKTLLKLLHQRCREE
                     GVKLHFEQNVDDLNQFADSDIIVACDGIGSAIRTQFASEFGTKIELKKNRFVWLGSTK
                     PLDAFTYFFRTTPFGTIVAHTYQYEEGMSTWIFECSNETWQNIGFEVTNEEDTIQKIA
                     EIFKDELDGHPLISNKSHWRQFPHVTNEKWSHNNIVLLGDAKATAHYSIGSGTKLAMD
                     CAIGLSDAVIANPNNVQVAFTQYDKTRRNTVEMIQYAALVSLDWFESMDRHRQHPFYQ
                     FAFGCMTRSKKVTYENLQIRDKSFTNKVLHEFNTLNNFQTELVEVSPAFSPFKLRNLE
                     LPNRIVMAPMGQYSAENGLVNNWHLVHYGSRATGGVGLILTEMTAVSSNGRITLGCAG
                     IYNENQLIEWKKITDFIHENTQTKIGIQLGHSGRKGAIKKPWEGQNEPIDKAWELHSA
                     SPIPFSSKLATPKEMTLEDMDSITAEFIQATKKAEQAGFDMIELQAHHGFLLASFLSP
                     LINIRTDEFGGSVDNRIKFPLRVFREMRKVFPQQKPMSVRISASDWVENGISEEDVIY
                     IANAFKNAGADIINVSTGNTVEYQKPLVGRMWQTPFS"
BASE COUNT         1605 a          865 c          877 g         1621 t
ORIGIN      
        1 tcaaattttc cttccaatac gctgtagcgt ttgcttgatt actcatagtt ttactgtttt
       61 aagggttagt tatagataaa tttgtaattg aacaatgaat tgtcctttgt acgaatcaat
      121 tgtatcaggt gttatataaa taggtcggtt ggaatactga gccgttattt ttgcatgatg
      181 tccgtctaaa aagaaattgg aaccaaaatc aaattgattg ctagcttggt ctaacgcatc
      241 aaagtttttg tatgtatatg ctgcaaaagg ttgaatacga actttaggtt tttcagcttt
      301 agatggtaat aaaaatcctg cttgtgtata ccagatattt cctgttccta tcgttggttg
      361 taaattcccg ggaccggcaa gtgcataatc acccgtgaaa gttgggtcaa ccgtaccttg
      421 gttcataata ccaatatttc ttaaataatt gggaccaaaa tcataattgt aaaaaacgct
      481 atatccagtg actgccattt ttttctcttt cggacctact ggcatatcta caaatacgtc
      541 ggcagataac aaattgatag agtgattttc aattacaccg ttcacagatg tttgagtagc
      601 ttttggagca ttataaaaac ctgctccaat attcaacatt ttttttgtcc ctaaataagt
      661 accaacttta aatggcagga agttagattc tttatcgaaa atttgatatt caaaatatcc
      721 cgctttagac cattgtgaag aattattgtt atcaacagct tttgcaattg cctcattgtt
      781 tacatcaatt ggtgtttgat tagttgcaaa aggtttattt aaactcaatc ggtattcaaa
      841 tttattgtat ttccctttag caaataaacc tatttgtcta gcaaactgat cggagttttc
      901 aatcaatggc cagttaaaaa tgggagcatc tacagccaag tagttcaaag tagaggacat
      961 tgtcatgcgg gaaagtccca tataataatg taaaccagct ccaatagaca aactaaatgc
     1021 tttgtcttct ttgggaagaa aaacagcata ttcattccaa gcatcgtgaa aaaacaaacc
     1081 tgctttttta cctgcaccat agcctccagt acctgatgaa cccgaagctc caccgttcaa
     1141 aaacgtctga ttattgattc ctatgtgagt tacaatcata taacgagttg atatttgtgc
     1201 atatgctaat aaacgtaaac gacgcaagcc aatgtcttca aaagtacttt gtggctcatc
     1261 accaatcatg gttccaggat ttaaatcggt ggaggtcaac caaatttgat tccaagctat
     1321 aaaacgcata tactttgtgc cttctggatt caaattaaat tttaagcccc cgccataatc
     1381 gagagaacct tgtgaaaatg cgttaatcgc tagtaacgca aagattccta aaaatagtgt
     1441 ttttttcatt gataataggg tttggttttt ggaaataatt gtttttttct gagtaccaaa
     1501 ttgaaatatt atctgaaaga aaaaaattca gctatatatt tttgttaatt actatagtta
     1561 atctttaaaa cgctcccatt ttataagcat aaatttgtct cctaattccg ttataatcat
     1621 tccggctccc gataaatgtt ctttattttt taagaattca actaactcta aatggttgta
     1681 tatcttgtag gtgggatgat aatcggtgtg ggcaggtttt tttattttat attctttcac
     1741 ccaattgctt acggtgacat gagaaactcc gataatccgt tcaatctcac ggtaacttaa
     1801 cccttccaag aacaattgca atgctttagt aacatagtaa tcgtctatct ttttacctag
     1861 tttattaacg gtaaaataat agttacattt tttgcataaa tacctttgtc tatctttgat
     1921 aacaccgctt tttacaattt gattactttg gcatttaggg caggctaaaa gttccatata
     1981 taaaaatttt gcataaatat actaaattag caattataca ataacatatt ctgtattttt
     2041 tttatgtttt ttttgtgaag tactcatttt tattgagcta aaattataaa ctatataatt
     2101 ttaccaataa tttatattgt tgctatttga agactatatt gcaattgaat ttaaaaaaag
     2161 ttaataattt taagaacata caacttatac tattccaact attttcttta atttgtaaaa
     2221 acaacactaa ctgatgcaat ttgaatcaaa caaaacggat ccttctgttt tatataaatt
     2281 attaaccgga atcattattc ccagacccat tggttggata tcaacaatag atagtaacgg
     2341 aatcaacaac cttgctcctt tttcctattt caatatggtt tccagtgacc caccttgtgt
     2401 gatgttttcc actcgaagag aaaacaataa aaacaaagac accttaaata acgttctgca
     2461 aaatggccaa tttgtggtga atttggtaac cttagaagta gttgaacaaa tgaatgcctc
     2521 ctctgaagcc tttcctgccg atgtggatga atttataata accaatttaa caccgattga
     2581 ttctgtttcc atccaaccca aacgtgtcaa agaaagtcct attcatttgg aatgcgaaat
     2641 gattcatcat tatttcatgg ataataacga aggtggcgga gcatgtatcg tcattggaaa
     2701 gattaaaacc attcatattg acgattcgat tttaatggaa aataaccgta tcaatttgga
     2761 tgtttacaaa ccggtggctc gtttagcagg ttcaaattat agtacgttag gggaactttt
     2821 ttcgatcaaa agagcataaa attttggaac actaaatgta ataattatag ataaaaacgt
     2881 cgcgtccttg cgactttgca aaaaatgaaa aaaataacag taattggcgg cggtcccgga
     2941 ggactctact tttccatact aactaaaaaa gcaatgcctc attgccnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnggtc ccggaggact ctacttttcc atactaacta
     3121 aaaaagcaat gcctcattgc caaatcgatg tgtacgaacg taacaaaccc gaagatagtt
     3181 tcggttttgg tgtcgtattt tccgatgaaa cattaggtga gtttttaatg cgggatttaa
     3241 aatcgtatga actcattcgt agtgaatttg cttattggga tgatattatt gtagctcgtg
     3301 atgggcagtc tgtaagcatc tccggtaacg gattttgtgg ttgttcgcgt aaaactttac
     3361 tgaaattatt gcaccaacga tgccgtgaag aaggtgttaa acttcatttt gaacaaaatg
     3421 ttgacgattt aaatcagttt gccgattcgg atatcattgt agcttgtgac ggaattggaa
     3481 gtgccattcg aacgcaattt gcttctgaat ttggcaccaa aattgaactc aagaaaaacc
     3541 gtttcgtatg gttgggttcc actaaaccgt tggatgcttt tacttatttt ttcagaacaa
     3601 cacctttcgg tacaatcgtg gctcacacct atcaatacga agaaggaatg agtacgtgga
     3661 tttttgaatg tagtaatgaa acttggcaaa atattggttt tgaagtcacc aatgaagaag
     3721 ataccattca aaaaattgct gaaattttca aagatgaatt agacggtcat ccactcattt
     3781 ctaataaatc acattggcga caatttccgc atgtaaccaa tgaaaaatgg tcacacaata
     3841 atatagtttt attgggcgat gcgaaagcca ctgcccacta ctccattggt tcgggaacaa
     3901 aattagcgat ggattgtgct attggtttat cagatgctgt aattgctaat ccgaataatg
     3961 ttcaagtggc ttttactcaa tacgacaaaa cccgcagaaa cacagtggaa atgattcaat
     4021 atgcagcttt ggtttccttg gattggtttg aaagcatgga ccgacatagg caacatccat
     4081 tttatcaatt tgcatttggc tgtatgacac gttccaaaaa agtgacgtat gaaaatcttc
     4141 aaattcggga taaatctttt accaataaag tgttgcatga attcaataca ttaaacaact
     4201 ttcagactga gcttgtagaa gtctctcccg ctttttcccc tttcaaatta agaaatttag
     4261 aattacccaa ccgaattgtg atggctccta tgggacaata ttcagctgaa aatggcttag
     4321 tcaataattg gcatttggtg cattacggaa gtcgagccac cggtggagtt ggattgattt
     4381 taaccgaaat gacagcagtt tcttcaaatg gacgtatcac tttaggttgc gcaggtattt
     4441 ataatgaaaa tcaattaatt gagtggaaaa agataactga ttttattcat gaaaatacac
     4501 aaaccaaaat cggaattcaa cttggacatt caggaagaaa aggagcaatt aaaaaacctt
     4561 gggaaggtca aaatgaaccg attgataagg cttgggaatt acattccgcc tctcctattc
     4621 cattcagttc caaattggcg actccaaagg aaatgacttt ggaagatatg gatagtatca
     4681 cagctgaatt tatccaagcc acaaaaaaag ctgaacaagc cggttttgat atgattgaat
     4741 tacaagctca tcacggattt ttattggctt cgtttttatc gcctttgata aatattcgaa
     4801 cggatgagtt tggtggaagt gttgataatc gaatcaaatt tccattgcga gtgtttaggg
     4861 aaatgcgtaa agtgtttcca caacaaaaac caatgtctgt ccggatttct gcgtctgatt
     4921 gggttgaaaa cggaatttca gaagaagacg taatttatat tgccaatgct ttcaaaaatg
     4981 ccggagcaga tatcatcaac gtttccaccg gaaatactgt tgaatatcaa aagccacttg
     5041 tcggcagaat gtggcaaaca cctttttc
//
LOCUS       DAOTCW010000158         4946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_13034_length_4946_cov_38.933143, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000158 DAOTCW010000000
VERSION     DAOTCW010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13034_length_4946_cov_38.933143"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..775
                     /gene="can"
                     /locus_tag="P5335_10360"
     CDS             140..775
                     /gene="can"
                     /locus_tag="P5335_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HRZ75323.1"
                     /translation="MSEFYKKILENNKNWVESKLKLDPNFFEGLSKGQSPPLLWIGCS
                     DSRVPANEIIGAAPGEVFVHRNIANMVIHTDMNMLSVLDYAVNALKVKHIIVCGHYGC
                     GGIKAAMGNNSIGIIDNWIRHIKDVYRFHQHELDDIQNENERFNKFVELNVREQVYDL
                     AKTSIVQSAWKTGQELSIHGWVYGLNSGYVTDLDVNFKNNDELDSVYQLKF"
     gene            893..1717
                     /locus_tag="P5335_10365"
     CDS             893..1717
                     /locus_tag="P5335_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HRZ75324.1"
                     /translation="MSKINIFFTALFSLLFCFQSYAQNDSIAKNIEELQIDTLKTDIQ
                     QFSLNTVNDSIAIIDSKLIYNPQRDSKRLLYNTGMYAGVAIASFGLLWIAPVSVSKWD
                     KEEIKDEGFATKWKDNVKAGPVVDEDDFYMNYIVHPWAGGVYYMSARGSGYKPWESFT
                     YSFLMSTFFWEYGIEAFAEIPSVQDLIITPVIGSIVGEGFFIAKGKIIKNERRILNSK
                     FLGNTTLFIMDPFNELLDVFGYKTKNKVVAYSSFIPIERNPFTNKQTWGMQMVLKF"
     gene            complement(1732..2928)
                     /locus_tag="P5335_10370"
     CDS             complement(1732..2928)
                     /locus_tag="P5335_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LETM1-related biofilm-associated protein"
                     /protein_id="HRZ75325.1"
                     /translation="MINPSAHGWIDKFFSKYYLLDSPYLIDSKDFYCRTRATGFIYGH
                     VISLDSSEPLLIKGCTSEEISKVALLNSLFGIYQLTAKNQDKVEFSKMVLKFYNTINP
                     QGFSLLKKILPNESDSIKLEKIIDERVQTNDNMVSKNFSHIVTNALLFIDVLAFQNFL
                     INNELSKNYIQKTEEIILGIVSLALTTKSNKTLYDDLLIKLFETSVRYTKFSGTKIDS
                     LDKLPLEILQTNLEKYYIMDLAAMAIWNDSEMEKKEAEFLHELGYTLQLEKSYIVECI
                     SAINQFINKYKADIPYFNYSNPIKHFYDQSTQNVVNLITRNKKRLQKELAESKELVVL
                     LATSTQRELDTQEKKKMKKQLLDVCKTIPSLTIFLLPGGSLLLPLLIKFIPQILPSSF
                     NENLDN"
     gene            complement(3011..4099)
                     /locus_tag="P5335_10375"
     CDS             complement(3011..4099)
                     /locus_tag="P5335_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRZ75326.1"
                     /translation="MSAKTILKKSSLTFLEKYLNNASPTGYESEGQKLWMEYIKPYVD
                     TFITDTYGTAVGIINPDAPYKVVIEGHSDEISWYVNYITDNGLIYVIRNGGSDHQIAP
                     SKRVNIHTKKGIVKGVFGWPAIHTRNRGKEETARIDNLFVDLGCSTKEEVEKLGVHVG
                     CVITYPDEFIILNENKFVCRAIDNRMGGFMVAEVARLLFENKKKLPFGLYITNSVQEE
                     VGLRGAEMITKTIKPNVAIVTDVCHDTTTPMIDKKIEGETQIGKGPVITYAPAVQNNL
                     RELIITTAEVNKIPFQRLASSRITGTDTDAFAYSNGGVASALISLPLRYMHTTVEMVH
                     REDVENVIKLIYETLLKMENNESFSYFK"
     gene            4185..4880
                     /locus_tag="P5335_10380"
     CDS             4185..4880
                     /locus_tag="P5335_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HRZ75327.1"
                     /translation="MKAFLSILFLCTVFIANSQEVKKDSLVIYYLIGNDSILRTSLDL
                     DEVVVTGHKSNKEDEDRKKFLLLQQRVIKVYPYAKLAADNLTRLNNNMGKFKTEREKK
                     KYFKIVENYLENEFEGQLKKLSRKQGQILVKLIYRQTGKSTYTLVKDLKSSWKAFWAS
                     NTAKVFDIDLKTEYDPFTVTEDFLIESILLKAFNEGRLQRQPSANFIDYVELSRIWKE
                     KVEKAKEKNNTTQ"
BASE COUNT         1723 a          694 c          774 g         1755 t
ORIGIN      
        1 gcaaaagaag aaggaataaa ggttaaatta gtaggcttta aaaaagcata taatttagat
       61 aacacagaag atggtcagca tatttcccta aaatcaagta aaataaaaaa agaaattgaa
      121 gtattaaaat aatagagata tgagtgagtt ttataaaaaa atattggaga ataataaaaa
      181 ttgggttgaa agtaagttaa aattagaccc caactttttt gaaggtttat caaaagggca
      241 atcacctcct ttgttatgga ttggttgttc agacagtcgt gtaccagcga atgaaataat
      301 cggtgcagca cccggagaag tttttgtaca tagaaatatt gcaaacatgg taattcatac
      361 ggatatgaat atgcttagtg tacttgatta tgccgtaaat gctctaaaag taaagcatat
      421 cattgtttgt ggccattatg gatgtggagg aataaaggct gctatgggaa ataactcaat
      481 tggaattatt gataattgga ttcgtcatat aaaagatgta tatcgtttcc atcaacatga
      541 attagatgat atacaaaatg aaaatgaacg atttaataag tttgttgaat tgaatgttag
      601 agaacaagta tatgatttag ctaaaacttc aattgtacag tcagcatgga aaacgggaca
      661 ggaattatca attcacggtt gggtgtatgg acttaattct ggatatgtaa cagatttaga
      721 cgttaatttt aaaaataatg atgaattaga tagtgtctat caacttaaat tttaattgtt
      781 tatattgaaa taaatgcatt tttcattttt aagaaattgt agcaaaatta ttttttaaaa
      841 aaagtatatt aaaattaagt ttttggtata ctttttttac ttttgcaaaa ttatgtcaaa
      901 aattaacatt tttttcacag cattattttc ccttcttttt tgttttcaat catatgcaca
      961 aaatgattca atagcaaaaa atattgaaga actacaaatc gataccttaa aaacggacat
     1021 ccaacaattt tcattaaata ctgttaatga ttcaatagca atcattgatt ccaaactaat
     1081 ttataatccc caacgagatt ctaaacggct tttgtacaat accggaatgt atgctggagt
     1141 tgctattgcc tctttcgggt tattatggat tgcaccggta agtgtttcaa aatgggataa
     1201 agaagaaata aaagatgaag gttttgcaac aaaatggaaa gataatgtaa aagctgggcc
     1261 agttgttgat gaagatgact tttatatgaa ctatattgtt catccttggg ctggcggggt
     1321 atactatatg tctgctagag gaagtggata taagccttgg gagtctttta cttattcttt
     1381 tttaatgtct accttttttt gggaatatgg gatagaagct tttgcagaaa taccttctgt
     1441 tcaagattta atcatcactc cagttattgg ttcaatcgtt ggcgaagggt tttttattgc
     1501 gaaaggaaaa attatcaaaa acgaaagaag gatattgaac tcaaaattct taggaaatac
     1561 tacacttttt atcatggatc ctttcaatga attattggat gtttttggct ataaaactaa
     1621 aaataaagta gtggcttatt caagttttat tccaattgag agaaatcctt tcaccaataa
     1681 acaaacttgg ggaatgcaga tggtattgaa attctaattt ttttaaattc tttagttgtc
     1741 taaattttca ttaaacgaag agggaagtat ttgtggaata aattttatta ataagggtaa
     1801 taataaactt ccacccggta ataaaaaaat agttagggaa ggaatggtct tgcaaacatc
     1861 taaaagctgt tttttcattt tctttttttc ctgagtatct aattcacgtt gagttgaagt
     1921 agctagtaaa actactaatt ctttactttc tgctagctct ttttgtagtc tttttttatt
     1981 tcgagttatc aaattaacta cattttgtgt cgattgatca taaaaatgtt taattggatt
     2041 tgaataatta aaataaggga tatcagcttt atacttattt ataaattgat taattgcgga
     2101 tatacattct acgatatagg atttttctaa ttgtagggta tagcccaatt catgtaaaaa
     2161 ttcagcttct tttttttcca tttcactatc attccaaata gccattgcag ccaaatccat
     2221 gatataatat ttttctaaat ttgtctgaag tatttctaaa ggtaatttgt ctaagctgtc
     2281 tatttttgta cctgaaaatt tagtatatcg cactgaagtt tcaaaaagtt taataagtaa
     2341 atcatcatac aatgttttat tactttttgt tgttaaagcc aaagaaacta tacctaaaat
     2401 aatttcttct gttttctgga tataattttt tgataattcg ttgttaataa gaaaattttg
     2461 gaaagcaaga acatcaataa ataataaggc attggtaaca atatgtgaaa agtttttact
     2521 taccatatta tcattggttt gaaccctttc atcaattatt ttttctaatt ttattgaatc
     2581 actttcattg ggtaatattt tttttaagag tgaaaaacct tgtggattta tcgtattata
     2641 aaatttcaaa accattttag aaaattcaac tttatcttga ttttttgcag tcaattgata
     2701 aataccaaat aaactattca ataaagctac ttttgaaatt tcttcagagg tacaaccttt
     2761 tatcaataaa ggttcagaag aatcaagcga tataacatga ccataaataa aaccggtagc
     2821 acgcgttctg caataaaaat ctttgctgtc aatcagatag ggtgaatcaa gcaaataata
     2881 tttgctaaaa aacttgtcta tccaaccatg tgcagacgga ttaatcattc actcaatttt
     2941 ttgcaaagct atggttttta aagcaaaaat tcccgaaaat caacttcggg aatcttaaaa
     3001 aaacaattgt ttatttgaaa taggaaaagg attcgttgtt ttccattttt aataaggttt
     3061 catatattaa tttaattacg ttttctacat cttcacggtg aaccatttca accgtagtat
     3121 gcatataacg taagggtaaa gagattaatg ccgacgctac accaccatta ctgtaagcaa
     3181 atgcatcagt atctgttccg gtaatccttg aagatgctaa tcgttgaaaa ggaattttat
     3241 tcacttcagc agttgtaatg attaattctc ttaaattatt ttgaactgcc ggagcataag
     3301 taataaccgg tcctttacca atttgtgttt cgccttcaat ttttttgtca atcattgggg
     3361 ttgtagtatc atgacaaaca tcagttacaa tggcaacatt gggtttgatg gtttttgtaa
     3421 tcatttctgc cccgcgtaac ccaacttctt cttgaacaga gtttgtaata tataatccaa
     3481 atggtaattt ctttttattt tcaaacaata aacgggcgac ttctgccacc ataaatccac
     3541 ccatacgatt atcaattgct cgacaaacaa atttgttttc gtttagaatt ataaattcat
     3601 ccggataagt aatcacacat cctacatgaa cgcctaattt ttcaacttct tcctttgttg
     3661 aacatcctaa atcaacaaat aaattgtcaa ttcgtgcagt ttcttccttt cctctatttc
     3721 gtgtatgtat ggctggccaa ccaaatactc ctttaacaat tcctttttta gtatgaatgt
     3781 taacccgttt tgagggtgca atttgatgat ctgaaccacc gtttctaatt acatagatta
     3841 ggccattgtc tgtaatgtaa tttacatacc atgaaatctc atctgaatga ccttcaatta
     3901 ctactttata aggagcatcc ggatttataa taccaactgc tgttccataa gtatctgtaa
     3961 taaaggtatc aacataaggt ttaatatatt ccatccatag tttttgacct tctgattcat
     4021 agcctgtagg cgaggcattg tttaaatatt tttctaaaaa agtgagggat gattttttta
     4081 gtatggtttt tgctgacata aattttgatt ttttttgcta aattataaat aagataacat
     4141 agttcagtat aaatctctat ttttgaactt tatttatgaa aaacatgaag gcctttttat
     4201 caattttatt tctgtgtact gtatttattg ccaattctca ggaagtaaaa aaagattccc
     4261 tagtcatata ttatttgatt ggaaatgact ccatattacg aacttcctta gatttagatg
     4321 aagttgtagt aaccggacat aaatccaata aagaggatga agaccgcaaa aagtttctat
     4381 tattacaaca acgggttatt aaagtttatc cttatgcgaa attggccgcc gacaatttga
     4441 cgcggttaaa taacaatatg ggtaaattta aaacagaacg tgaaaaaaag aaatacttta
     4501 agattgttga aaactacctt gaaaatgaat ttgaaggcca acttaaaaaa ttatctcgta
     4561 aacaaggtca aattttagtc aaattaattt atcgccaaac aggaaaatcg acctatacat
     4621 tagttaaaga tttaaaaagt agttggaaag ccttttgggc aagtaatact gcaaaagttt
     4681 ttgacataga tttaaaaaca gaatatgacc cttttactgt aacggaagat tttcttatcg
     4741 aaagtatttt gttaaaagct tttaatgaag gccgtttgca acgtcaacct tcagccaact
     4801 ttatcgatta tgttgagctt tccagaatat ggaaagaaaa agttgaaaag gcaaaagaaa
     4861 aaaataatac tactcaatag tattttttaa ttaccaattc acaagtttta ttaattatca
     4921 tatttatttt ttagatataa tataag
//
LOCUS       DAOTCW010000159         4930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_13093_length_4930_cov_50.961846, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000159 DAOTCW010000000
VERSION     DAOTCW010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13093_length_4930_cov_50.961846"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..522)
                     /locus_tag="P5335_10385"
     CDS             complement(52..522)
                     /locus_tag="P5335_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRZ75328.1"
                     /translation="MKINSLQIEIDGIDKEILRDLMEDARKPILQIANKIGISGAAIH
                     QRLRKLEDAGVISGSKFIINNKVLGYKTMAFIGVYLDKASSNSEAVKELKKIPEVLEC
                     HYTTGNWSILIKIICRDNEHLMQLLNKKIQPIDGVSRTETFISLEQQIERQIQL"
     gene            718..1317
                     /locus_tag="P5335_10390"
     CDS             718..1317
                     /locus_tag="P5335_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N1)-methyltransferase"
                     /protein_id="HRZ75329.1"
                     /translation="MKNYFYFFLLLLTNVALSQEPNPESNSASINGTLANQFDYVIQK
                     SNNFQEYKVVKRDYLMLLKKNSLDSVGRFKNELVSLKSQFSNHASIVAQLNDTLKATN
                     EELKTLKTAQDNVSLFGSPISKTNYNIVMWGIVIVLFLILIVFLFQLKSAKSIAHEVK
                     NNVEKIEEEFEDYKHKALEKEQKLGRQLQDEINKHKSSK"
     gene            complement(1323..2693)
                     /locus_tag="P5335_10395"
     CDS             complement(1323..2693)
                     /locus_tag="P5335_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRZ75330.1"
                     /translation="MRTILIIGAGRSASSLIKYLLNKAETERLHLIIADLSLELAQKK
                     TNNHPNATPIQLDIANIEERQQQIQKADIVISMLPAHLHVEIAEDCIIFKKHMVTASY
                     ISPKMQELDAQAKENGLVFMNEIGLDPGIDHMSAMKVIDEIREKGGKMIQFESFCGGL
                     VAPESDTNLWNYKFSWNPRNVVLAGQGGVSKFIQEGKYKYIPYNKLFRRTEFLEVEGF
                     GRFEGYANRDSLKYRSIYGLDNILTMYRGTLRRVGFSKAWDMLVQLGMTDDGYTMENS
                     ENMSYRDFTNSFLAYHPTDSVELKLRHYLKIDQDDVIWDKFMELDLFDSSKKIGLKNA
                     TPAQILEKILAEKWALQPNDKDMIVMYHKFGYEMNGQQKQIDSTMVCLGDDQIYTAMA
                     KTVGLPVAMATLQILNGNIKTPGVQLPIKKEVYEPILNELETFGVIFIEKEVPYMGYN
                     PNNVGS"
     gene            2815..3201
                     /locus_tag="P5335_10400"
     CDS             2815..3201
                     /locus_tag="P5335_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HRZ75331.1"
                     /translation="MDRKIILTATFLGTLSIVLGAFGAHALKAVLAENQLITFETGIR
                     YQMYHAFFLLFLGTTSLVPEKTKKIILYLVLVGILFFSGSIYLLATNSLTSIDFKFVG
                     PITPIGGLLLIAAWTVLFLYFLKRKG"
     gene            3280..4905
                     /gene="pckA"
                     /locus_tag="P5335_10405"
     CDS             3280..4905
                     /gene="pckA"
                     /locus_tag="P5335_10405"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRZ75332.1"
                     /translation="METYAQFTKSISLEKYGIKNTQEIIYNPSFEFLYKEEMNPNLEG
                     YEKGQLSELGAVNVMTGEFTGRSPKDKYIVEDEVTKDTIWWNSPKAPNDNKPISTATW
                     NALKQTTVSQLSGKRLFVVDAFCGANENTRLKVRFIMEVAWQAHFVKNMFIRPTEKEL
                     ENFGEPDFIVMNGSKTSFKDYAAHGLNSEVYVAFNLTEKVQIIGGTWYGGEMKKGLFS
                     MMNYYLPLQGIASMHCSANKGKDGDVAVFFGLSGTGKTTLSTDPKRELIGDDEHGWDD
                     EGVFNFEGGCYAKTIDLSKENEPDIYGAIKKDALLENVTLDENGKIDFKDGSVTQNTR
                     VSYPIYHIDNIVRPVSKAGHATKVIFLTADAFGVMPPVSKLTPEQTKYYFLSGFTAKL
                     AGTERGVTEPQPTFSACFGKAFLSLHPTKYGEELVKKMEEHKATAYMVNTGWNGTGKR
                     ISIKDTRAIIDAILDGSIEKAASKVIPVFNFEVPTALHDVNPAILDPRDTYSTVAEWN
                     EKATNLAGLFIKNFVQYTDNEEGQNLVKSGPQL"
BASE COUNT         1657 a          789 c          814 g         1670 t
ORIGIN      
        1 aatccgttac gaaagtaacg gattttttat tttacatttt cacacaaaac tttacaattg
       61 aatttgtctt tcaatttgtt gttccagcga aatgaacgtt tctgttctag aaaccccgtc
      121 aataggttgg atttttttat tcaataattg catcaaatgt tcgttatcac gacaaataat
      181 tttgattaat atactccaat ttccggtggt ataatgacat tccagtactt cgggaatttt
      241 tttaagttct tttacagctt ctgaattact agaagctttg tctaaataaa caccaataaa
      301 agccatggtt ttatacccaa gaactttgtt gtttatgata aattttgaac ccgaaatcac
      361 tccggcatct tccagttttc gtagccgttg atgaatagct gctccggaaa tccctatttt
      421 attggcaatt tgtaaaatgg gttttctggc atcttccatc aaatcacgaa gaatttcttt
      481 gtcgattcca tctatttcaa tttgaaggga attgattttc atagttttaa attttaatca
      541 aaaataacaa aaattgacaa taagtaagta aaattataag ttttgattta aaacagtaag
      601 tacaataatt ttcttttttt tacgatttat ccgtgatatt aggtttccta gtcaaaaaaa
      661 attaaatttg ttaacctact tgaataaaag agttagttta aatcaatttg taatcgtatg
      721 aaaaactatt tttacttctt tttgttgttg ctaactaatg ttgcattatc ccaagaacct
      781 aatccggaat caaattctgc atcgatcaac ggaactttag cgaatcaatt tgactatgtg
      841 attcaaaagt caaataattt tcaagagtat aaagtggtta aacgagatta tctgatgtta
      901 ttaaaaaaaa acagtttaga ttccgttggt agattcaaaa atgagttagt aagtttaaaa
      961 agtcaatttt ctaatcatgc ttccattgtt gctcaattga atgacacttt aaaagctaca
     1021 aatgaagaat taaaaacctt aaaaacagca caagataatg tttcgctttt tggaagccca
     1081 atcagtaaaa ccaactataa tattgtgatg tggggaattg ttattgtatt gtttttaatt
     1141 ttgattgttt ttttattcca acttaaaagt gctaaatcaa ttgctcatga agtaaaaaat
     1201 aatgttgaaa aaattgaaga agagtttgaa gattacaagc ataaagcatt ggaaaaagaa
     1261 caaaaattag gacgtcaact tcaggacgaa attaataaac acaaatcttc taaataaaat
     1321 ttttaacttc ctacattatt tgggttataa cccatgtaag gcacttcttt ttcaataaaa
     1381 attacgccaa aagtttctag ttcgtttaaa ataggttcat aaacttcctt tttaatgggc
     1441 aattgaactc cgggtgtttt tatgtttcca tttaagattt gaagtgttgc cattgccacc
     1501 ggtaaaccta ctgttttagc catagcggta taaatctggt catcacctaa acataccata
     1561 gtgctgtcaa tttgcttttg ttgaccattc atttcataac caaatttgtg gtacatcaca
     1621 atcatatctt tatcatttgg ttgtaaagcc catttttcag ctaatatttt ttccagaatt
     1681 tgagcaggag tggcattttt tagtcctatt ttttttgagg aatcaaataa atcaagctcc
     1741 ataaacttat cccaaattac atcatcctga tcaattttaa gataatggcg gagttttaat
     1801 tcaacagaat ctgttggatg ataagctaaa aaagaatttg taaaatcacg gtaactcata
     1861 ttttctgagt tttccatggt ataaccgtcg tccgtcattc ctaattgtac taacatatcc
     1921 caagctttag aaaaaccaac acgtctaagg gttcctcgat acatggttaa aatgttatct
     1981 aaaccataaa tgcttctgta tttcaaagaa tcacgattag catatccttc aaaacgaccg
     2041 aatccttcta cttctaaaaa ttcagttctg cggaataatt tgttgtaagg aatgtattta
     2101 tatttacctt cctgtatgaa tttagaaaca ccgccttgcc cggccaaaac tacatttctt
     2161 ggattccagg aaaatttata attccataaa ttagtgtcgc tttcaggggc aaccagtccg
     2221 ccacaaaacg attcgaactg aatcattttt cctccttttt ccctgatttc atcaattact
     2281 ttcatcgcac tcatgtggtc gatacccgga tctaaaccaa tttcgttcat gaaaaccaag
     2341 ccattttctt tggcttgagc atccaattct tgcatttttg gacttatata agaagccgta
     2401 accatgtgtt ttttgaaaat aatgcaatct tcagctattt ccacatgaag atgagccgga
     2461 agcattgaaa tgacaatatc tgctttttga atttgctgtt ggcgttcttc tatatttgca
     2521 atatccaatt gaattggcgt tgcgttaggg tgattattgg tctttttttg agctaattct
     2581 aatgataaat ctgcaattat taaatgcaaa cgctctgttt cagctttgtt taataaatat
     2641 tttattaggg atgaagccga tcttccggca ccaataatca ggatagttct catgttgtct
     2701 ttcggtaaaa atcacacgaa agtaagcaaa tgttatattc ctaacaaatt taggtgtgtt
     2761 aattcttctt atgtgtattg tataaaacct aaatttgtgc ttcaatttat aattatggac
     2821 agaaaaatca ttttaacagc aaccttttta ggaactttgt ctattgttct tggagctttt
     2881 ggagcacatg ctttgaaagc tgttcttgcg gaaaatcaac tcattacttt tgaaacgggt
     2941 atacgttatc aaatgtatca tgccttcttt ttattgtttt tgggaacaac ttctttagta
     3001 cccgaaaaaa caaaaaagat aattttatat ttagttttag taggcatttt atttttttca
     3061 gggtctatct atttattagc cacaaattca cttacaagta ttgattttaa gtttgttgga
     3121 ccaattacgc ctattggagg attgttatta attgctgcct ggactgtgct ttttttatat
     3181 tttttgaaac gaaaaggata aaatatttat aattcttcaa tcgtaatgaa ttaattttta
     3241 cttttgccaa ataattaaac acaactaaac ggtaaattta tggagacata cgctcaattt
     3301 acgaaatcga tttcgttaga aaaatacggt atcaaaaata cacaagagat tatttacaat
     3361 ccatcttttg aatttttata caaggaagaa atgaatccca atttagaagg atatgaaaaa
     3421 ggacaattgt cagaattagg agctgtaaat gtaatgacgg gagaatttac aggaagatcc
     3481 ccaaaagaca agtatattgt agaagatgag gtgactaaag atacgatttg gtggaattct
     3541 ccaaaagctc caaatgataa caaaccaatt tctacagcta catggaatgc tttaaaacaa
     3601 acaacagtaa gccaactttc aggaaaacgc ttatttgtag ttgatgcatt ttgtggtgcc
     3661 aatgaaaaca caagattgaa agttcgtttc attatggaag tcgcttggca ggctcatttt
     3721 gtgaaaaata tgtttattcg tccaacagaa aaagaattag aaaattttgg agaacctgat
     3781 ttcatagtga tgaatggatc aaaaacttct tttaaagatt atgcggcaca cggattgaac
     3841 tctgaagttt atgttgcctt taacttaacg gaaaaagttc aaatcattgg aggaacttgg
     3901 tatggtggtg aaatgaaaaa aggcttgttc tcgatgatga attactattt accattacaa
     3961 ggaattgctt ccatgcattg ttctgcaaac aaaggaaaag acggtgatgt agctgttttc
     4021 tttggtttgt ctggaacagg aaaaacaact ttatctactg atccaaaacg agaattaatt
     4081 ggcgatgatg agcacggttg ggatgatgaa ggcgttttta acttcgaagg ggggtgttat
     4141 gccaaaacga ttgatttaag caaagaaaat gaaccggata tttacggagc tatcaaaaaa
     4201 gatgctttgt tagaaaacgt aacgttagat gaaaatggaa aaatcgactt taaagatggt
     4261 tctgtaacac aaaatacacg tgtttcttat cctatttacc acatcgataa tattgtacga
     4321 cctgtttcta aagcaggtca tgcaaccaaa gttattttct taactgcaga tgcttttggt
     4381 gttatgccgc cggtttctaa attaactccg gaacaaacaa aatattattt tctttccgga
     4441 tttacagcaa aattagccgg aacagaaaga ggagttactg aaccacaacc tactttttcg
     4501 gcttgtttcg gaaaagcgtt tctgtcgctt catccaacaa aatatggtga agaattggta
     4561 aaaaagatgg aagagcacaa agctacagct tatatggtta acaccggatg gaatggaacc
     4621 gggaaacgta tttccataaa agataccaga gcaattatcg atgctattct agatggttca
     4681 attgaaaaag cagcatcaaa agtaattccg gttttcaatt ttgaagtgcc aacagctcta
     4741 cacgacgtaa atcccgcaat attagatcca agagatactt attcaactgt tgctgagtgg
     4801 aatgaaaaag caactaactt ggctggatta tttattaaaa atttcgtaca atatacagat
     4861 aatgaagaag gacaaaattt agtgaagtct ggacctcaat tataatttta tactaactaa
     4921 ccaaacgaaa
//
LOCUS       DAOTCW010000160         4881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_13277_length_4881_cov_46.991919, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000160 DAOTCW010000000
VERSION     DAOTCW010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13277_length_4881_cov_46.991919"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1396)
                     /gene="tilS"
                     /locus_tag="P5335_10410"
     CDS             complement(92..1396)
                     /gene="tilS"
                     /locus_tag="P5335_10410"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRZ75333.1"
                     /translation="MFSKFQNHLSTNFSFLREAKILVAVSSGLDSMVLLKLLSNSNLQ
                     IAVAHCNFQLRDIESDDDEVFVKSYCHNNSFQGFFQKFDTKKFAKDEKLSIQLAARKL
                     RYEWFYELLEKENFDFVLTAHHLDDQLETFLINFSRGTGLEGLTGIPSQNDKIIRPLL
                     VFTREEIENFANENNISWREDSSNSSDKYLRNKIRHQIVPILKELNPSFLDSFQKTIQ
                     NLNQAQSLVDDASRIVYRKVVKEDENQKIIDLNELLQLSNYQAYLYQWLSPFGFSAWQ
                     DIYDLAKAQSGKQIFSEHFRLVKHRETLLLESKAVKNEEEYTIADNQSELNFPLKLRF
                     NSVSNISISDSKTIFVDAVALKFPLKLRKWQEGDYFYPFGMSGKKKLSKFFKDEKFSL
                     IEKENSWLLCSENQIVWLVGKRLDNRFKVTENSQTIIKIQLV"
     gene            complement(1439..2743)
                     /locus_tag="P5335_10415"
     CDS             complement(1439..2743)
                     /locus_tag="P5335_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRZ75334.1"
                     /translation="MLRTVKSFTLETPESFKNNLLEWANQFKEVVFLDTNKYHQKYST
                     YDLVLAVDAFTSIKTDYFNAFEDLQQYQSHTKDWLFGYLSYDLKNDTEALTSKNFDGL
                     AYPDLYFFQPKKLFFLKENQLEVQYLRLCDDEITDDFSAILNANAASDLTPESSGLGE
                     IKIQQRISKEAYLEKVSKMLEHIHHGDIYEANFCMEFYSEEVQINPLEIYQKLNKISE
                     PPFAVYFKNSHHYLLSASPERYLKKEGTKVISQPIKGTSRRIPDPILDEQSKIDLENN
                     PKERAENIMIVDLVRNDLSRTATKGSVEVEELCKIYTFKQVHQMISTVVSQVEITTSP
                     VEILKTTFPMGSMTGAPKISAMQIIEKLEETKRGLYSGAVGYFTTEGDFDFNVVIRSI
                     LYNAENQYLSFSVGSAITSEATPEGEYEECLLKAKAMFEVLK"
     gene            complement(2751..3290)
                     /locus_tag="P5335_10420"
     CDS             complement(2751..3290)
                     /locus_tag="P5335_10420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRZ75335.1"
                     /translation="MSTLLAFAGSNSSTSINHELVTYLTTQLKESTFELFKLSDMDLI
                     VYSEDEQRENGFPSSINQIYKHIQASEGIIISVNEHNSNPSAFFKNVLDWLSRLDRKF
                     LEGKKVFLVSASTGARGGMSSLEVVKNLIPRFGGEVVDTFSFPSFKENFSLTEKAITN
                     EELKIEILLKLHQFENKLK"
     gene            complement(3420..4823)
                     /gene="lpdA"
                     /locus_tag="P5335_10425"
     CDS             complement(3420..4823)
                     /gene="lpdA"
                     /locus_tag="P5335_10425"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRZ75336.1"
                     /translation="MSQFDVAIIGSGPGGYVAAIRCAQLGFKTAIIEKYSVLGGTCLN
                     VGCIPSKALLSSSHHYHDAVAHFEEHGIEVGKVKVNLEKMIARKQAVVDQTTGGIKFL
                     MDKNKITVFEGIGSFEDATHVKVTKNDGSSETIEAKYSIIATGSKPSNLPFITLDKER
                     VITSTEALKLPEIPKHLVIIGGGVIGIELGQVYLRLGSQVSVVEFMDRIIPGMDASLS
                     KELTKVLKKQGMKFYTSHKVKSVNRKGKTVTVEAETPKGEILSLEGDYCLVSVGRRPY
                     TDGLNSEKAGVKVTDRGQIEVNNHLQTTAANIYAIGDVVRGAMLAHKAEEEGVLVAEI
                     LAGQKPHIDYNLIPGVVYTWPEVAAVGKTEEQLKEEGVAFKSGSFPFKALGRARAGGD
                     TDGFVKILADAKTDEVLGVHMIGPRCADLIAEAVTAMEFRASAEDISRMSHAHPTFAE
                     AVKEAALAATDNRPLHV"
BASE COUNT         1545 a          897 c          771 g         1668 t
ORIGIN      
        1 attgacgttt tccatttgac cggatctaaa atttgtgctt gtgtaattgt aattacaaaa
       61 aaagaaagca acgtaaaaaa taaattcttc attatactaa ttgaattttt atgattgttt
      121 gtgagttttc ggttacttta aatcgattgt ctaatcgctt tccaacaagc caaacaattt
      181 gattttcaga acataaaagc caagaatttt ctttttctat taaagaaaat ttttcgtctt
      241 taaaaaattt actaagcttc ttttttccac tcattccaaa agggtaaaaa taatcacctt
      301 cttgccattt cctaagtttt aaaggaaatt ttaacgcaac tgcgtctaca aagatagttt
      361 ttgaatctga tattgaaata ttgcttacgc tattaaaccg caactttaag ggaaaattaa
      421 gttccgattg attatcagca atagtatatt cctcctcatt tttaacagct ttggattcta
      481 aaaggagtgt ttctctatgt ttaactaatc tgaaatgctc agaaaagatt tgttttcctg
      541 attgagcttt cgccaaatca taaatatcct gccatgctga aaatccgaaa ggtgataacc
      601 attgatacaa atacgcttgg taatttgata attgaagtag ttcatttaag tcgattatct
      661 tttgattttc atcctctttc accacttttc gatagacaat tcgcgaggca tcatccacca
      721 acgattgtgc ttgattgaga ttttgaattg ttttttgaaa agaatctaaa aagctcggat
      781 tcaattcttt taaaattgga actatttgat ggcgaatttt atttcgtaaa tatttatcag
      841 aagaattact actatcttct cgccaagaaa tgttattttc attcgcaaaa ttttcaattt
      901 cttctctggt aaaaaccaac aagggtcgga taattttatc attttgggag ggaattccag
      961 tcagtccttc caaacctgtt ccgcgagaaa aattgattaa aaacgtttct aattgatcgt
     1021 ctaaatgatg agcggttaaa acaaaatcaa aattttcttt ttccaataat tcataaaacc
     1081 attcatatcg aagttttcgg gctgcgagtt gaatggataa tttttcatct ttggcaaatt
     1141 ttttggtatc aaatttttga aaaaaacctt gaaaagaatt attatgacaa tacgatttca
     1201 caaaaacctc atcatcatca ctttcaatat cacgaagttg aaaattgcaa tgggctacag
     1261 caatttgtaa atttgaattc gataataatt tcagtaaaac catactatcc aaaccactac
     1321 tgaccgcaac aagaatttta gcctctctta gaaaggaaaa attagttgac aaatggtttt
     1381 gaaatttaga aaacattttc aaaagtaagg atttttgaga gaatgaaata atgagcaact
     1441 atttcaatac ttcaaacatt gcttttgctt tcaacaaaca ttcttcatat tcgccttcgg
     1501 gagtcgcttc agatgtgatg gcacttccaa cggaaaaaga taggtattga ttttcagcat
     1561 tatataaaat actgcgaatc acaacattaa aatcaaaatc accttcggta gtgaaataac
     1621 caacggctcc gctgtataat cctcgtttgg tttcttctaa tttttcaata atctgcatcg
     1681 cagaaatttt tggagcaccg gtcatacttc ccatcgggaa agtagttttg agaatttcaa
     1741 ccggtgaagt ggtgatttct acttgagaaa caacagtaga aatcatttga tgcacttgct
     1801 taaaagtata aattttgcac aactcttcta cttctaccga acctttggta gccgttcttg
     1861 acaaatcatt gcgaaccaaa tctacaatca taatattttc ggctcgttct ttcggattat
     1921 tttctaaatc aattttcgat tgttcatcta aaattgggtc cggaattctt ctggatgttc
     1981 ctttgatggg ttgcgaaata actttggttc cttccttttt taaatacctt tcgggtgaag
     2041 cggataataa ataatgatga ctatttttaa aataaacagc aaaaggcggt tcagaaattt
     2101 tatttagttt ttgatagatt tccaaagggt taatttgtac ttcttcggaa taaaattcca
     2161 tgcaaaaatt agcttcataa atgtcgccgt gatgaatatg ttctagcatt ttggaaactt
     2221 tctcgaggta agcttccttt gaaattcttt gttgaatttt aatctctccc aatcccgaag
     2281 attcgggagt taagtcggaa gctgcattcg catttaaaat tgctgaaaaa tcatcggtta
     2341 tttcatcatc acaaagtctt aaatactgaa cttccaactg attttctttc aaaaagaaca
     2401 actttttagg ttgaaagaaa tacaaatcag gataggctaa tccgtcaaaa tttttagaag
     2461 ttaatgcttc ggtatcattt tttaaatcat acgaaaggta gccaaataac cagtctttgg
     2521 tatgtgattg gtattgttgt aagtcttcaa aagcattaaa ataatcggtt ttgatggaag
     2581 taaaagcatc tactgccaaa accaaatcat aagtcgaata tttttgatgg tacttattcg
     2641 tgtctaaaaa aacaacttct ttgaattgat tggcccattc caacaaatta tttttgaagg
     2701 attccggagt ttcaagagta aaacttttga cagttcttaa catcgaacag ttatttcaat
     2761 ttattttcga attggtgcaa ttttaataaa atctctattt tcaattcttc gttggtgatt
     2821 gctttttctg ttaaagaaaa gttttcttta aacgaaggaa aggaaaatgt atcaacaact
     2881 tcaccaccaa aacgtggaat caaattttta actacttcca acgaactcat acctcctctt
     2941 gctccagttg aagcactaac aagaaatact tttttacctt caaggaattt tcgatccaat
     3001 cgcgacagcc aatccaacac atttttaaag aatgcagaag gattactatt gtgttcattc
     3061 acagaaataa taattccttc agaagcttga atatgcttat aaatttgatt gattgaagat
     3121 ggaaaaccat tttcgcgttg ctcatcttca ctataaacaa tcaggtccat atcagacaat
     3181 ttgaataatt caaacgttga ctctttcaat tgagtagtca aataggttac taattcgtga
     3241 ttgattgagg tggaagaatt acttccggca aaggctaaga gggttgacat ggctattttt
     3301 tttgtaaagg taaggaagaa ttttattgtt acaaacctta aataaaatga cactaaaaga
     3361 aaaccccgtc agagttttga atgttgacgg ggttaaaaat aaaaaagttg aattatattc
     3421 tacacatgca acggacgatt atccgtagcc gccaaagccg cttcttttac tgcttctgca
     3481 aaagttggat gagcgtgact cattcttgaa atatcttcgg cactagctct aaattccatt
     3541 gctgttacag cttctgctat taaatcggca caacgtgggc caatcatgtg aacgcctaac
     3601 acttcatcag ttttagcatc ggctaagatt tttacaaatc cgtcggtatc tccgccggct
     3661 cttgctcttc ccaaagcttt aaatgggaaa cttccagatt taaaggctac tccttcttct
     3721 tttaattgct cttctgtttt tccaacagcg gcaacttccg gccaagtgta aaccacaccc
     3781 ggaattaaat tataatcgat atgcggtttc tgtccggcta aaatttcggc aaccagtaca
     3841 ccttcttctt ccgctttgtg agctaacatg gctccacgaa ctacatcgcc aatagcataa
     3901 atatttgctg cggttgtttg caagtgattg tttacttcaa tctgacctct atcggttact
     3961 tttactcctg ccttttcgga attcaaacca tccgtgtaag gtcttctacc cacagaaacc
     4021 aagcaataat ctccttctaa cgataaaatt tctccttttg gagtttcggc ttcaacagtt
     4081 acggtttttc ctttgcggtt aaccgatttt actttatggg aagtgtaaaa tttcattcct
     4141 tgttttttca acactttggt cagttctttc gacaaggaag catccattcc cggaataatt
     4201 cggtccataa attctactac agaaacttgt gaacccaaac gaagataaac ttgccctaat
     4261 tcgattccga ttacacctcc gccaataatg actagatgtt taggaatttc aggtaatttc
     4321 aatgcttcag ttgaagtgat aactctttct ttgtctaatg taataaatgg tagattcgat
     4381 ggttttgaac ctgttgcaat gattgaatac ttggcttcaa tagtttctga ggaaccatca
     4441 tttttggtta ctttcacgtg tgtagcatct tcaaacgatc caattccttc aaaaacagta
     4501 attttatttt tatccatcaa aaacttaatt ccacccgttg tttgatcaac aacggcttgt
     4561 tttcgggcta tcattttttc cagatttact ttcactttac ccacttcgat accgtgctct
     4621 tcaaaatgag ctactgcatc atggtaatga tgagaagaag aaagcaaagc tttagaggga
     4681 atacatccca catttaagca agttccgcct aaaaccgaat atttttcgat aatagccgtt
     4741 ttaaatccta attgtgcaca acgaatggca gccacatatc ctcctggacc ggaaccgatg
     4801 atagcaacat caaattgact catatgtttt tatttagtag ttaaatatca gttaaacgac
     4861 tgcaaaatta cgaatgtttt t
//
LOCUS       DAOTCW010000161         4795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_13607_length_4795_cov_26.980380, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000161 DAOTCW010000000
VERSION     DAOTCW010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13607_length_4795_cov_26.980380"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..813)
                     /locus_tag="P5335_10430"
     CDS             complement(28..813)
                     /locus_tag="P5335_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75337.1"
                     /translation="MISKSLILDLTELLAVGSVTTAGYLIGGTVGASVMAGLGINLAS
                     TIIGNGSSKLKARWIESKDGILNHDMQRALLRAYLKSLSHIKDCYFSENYDLPKQDKK
                     QINQLIESLTKTASTTFIPDIEKSIQQTEITQYLYGNQNEGEELLHNRIKSVSYLDEY
                     GESFKSYFLSKILSEIKFWFGEELKTDSIDCNKAWRAFQRLLLEGIKVELTDVKASQK
                     EIKADLSRLTRIEEDINKLTNVIDSRASNEPFLLNSNLDINTY"
     gene            complement(846..1775)
                     /locus_tag="P5335_10435"
     CDS             complement(846..1775)
                     /locus_tag="P5335_10435"
                     /inference="COORDINATES: protein motif:HMM:NF039447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6430 domain-containing protein"
                     /protein_id="HRZ75338.1"
                     /translation="MIKRFLTIFTKNYWKYIFSIKKTFNAALGTLGIIWLLIEPTSYF
                     SQGLSDFFKAHWLWLLVLGGIWVIYENWPKTEFCYKLNERDIRIQISIGNLFDYKGHL
                     VIPINTSYDTSFEGDLIANTSTQGQFTIKYFKEPRYLDQDLTIALACEPPTTQLPQKN
                     RGNKYRYDIGKVLKLKLENDKYAFLSAISDMNNNGVAETNFDNIQTSLGSLWSYVSTN
                     GESGQINIPIIGTGRGRLIDSREKVIKSIINSFVSATTETERRFCDKLNIVIHPKDFS
                     EHQINIDELTRYLELKCDFYEYDTRRTGVGQAI"
     gene            complement(1772..2284)
                     /locus_tag="P5335_10440"
     CDS             complement(1772..2284)
                     /locus_tag="P5335_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HRZ75339.1"
                     /translation="MAYRNKTYLCFDGDNDIHYFYLMKAWKHNQNDFFKDFNFYDAHE
                     INYARDSSKEESIKRQLLERLNSSNLFVVLVGEQTKFLTKFVRWEMEQALKQEIPIVA
                     INLNGKRELDDNRCPVILKDKLVLHISFNQKIIEKAMSEWREISQNLKREAKSGPFSY
                     KPTVYSQLGL"
     gene            complement(2473..3321)
                     /locus_tag="P5335_10445"
     CDS             complement(2473..3321)
                     /locus_tag="P5335_10445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRZ75340.1"
                     /translation="MIKQSYRFLNPKFQNLFLEYKVDFKPRYGFGKPPHKDLYKIIDS
                     NRDYYKQLIDKSLLLKDFIWTIKDSSNETDSTKPTWNNGFLPGLDIIGIYILLTEFKP
                     KKYIEIGSGNSTKVAYKTKTEQNLDTEIISIDPMPRAEIDNLANKVIRKPFENIDFDI
                     LDELNENDILFVDNSHRILPNSDSMVFYLEILPRLKKGVIVHIHDIYLPYDYPQFMCD
                     RFYSEQYGLAMYLLANPKKYDTIFPNYFIFEDKELSDLISPIWNHVNLKEVEKHGGSY
                     WIKINE"
     gene            complement(3509..4381)
                     /locus_tag="P5335_10450"
     CDS             complement(3509..4381)
                     /locus_tag="P5335_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75341.1"
                     /translation="MKGFEYILAKQVQWAKRKNISLIGSKVDKGFPAYTKKLEENLFE
                     PLAADVIADFSKGDGGEILNKNNSPAKMNAVHSSSALGVNLFHYWKTTGKIDNISHAC
                     GLCAKQNKTQTDINFEQKFIISKAFPFCPNLDVVIENNDKAQYKVFAIECKYSEAYSN
                     HGHSGVDPKYFNNDILPLWKDIPKLYELAKAISPDDSDFKFLHPAQLIKHILGLKNKY
                     GKKSFRLLYLWYDVLGEEGYIHRNEIDKFSEYTKADDIKFSSISYQELITFLADNYYD
                     TNEKYIEYITDRYL"
     gene            complement(4390..>4795)
                     /locus_tag="P5335_10455"
     CDS             complement(4390..>4795)
                     /locus_tag="P5335_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75342.1"
                     /translation="IGTSLITEITTYLLSGNKRWLKFRKIYTRFYSFLISSVAWLITT
                     GIFISKEFSDFSYGVNAIIRFPVDLVKMLFSARTGAEYENPDTLLQWKVLLISAGICL
                     VIYLIVLFASRASAKKVSPIPPEVNMDNLNKY"
BASE COUNT         1426 a          859 c          767 g         1743 t
ORIGIN      
        1 tcaagcattt tcatcaaaac ttataaacta atacgtgtta atatccaggt tagagtttaa
       61 tagaaaaggc tcattggaag ctcgactatc aataacatta gtcaacttat tgatatcttc
      121 ctcaattctt gtgagccgag ataaatctgc ctttatttct ttttgactgg cttttacatc
      181 tgtaagttct acttttatcc cttctaaaag caacctttga aatgctctcc acgctttatt
      241 gcagtcgata ctatcagttt ttaattcttc accgaaccag aattttattt cggacaagat
      301 ctttgaaaga aaatagcttt taaatgattc gccatattca tcaagataac taactgattt
      361 aatcctatta tgcaacaatt cttcaccttc gttttgattc ccatacaaat attgggtaat
      421 ttcagtttgt tgaattgatt tttcaatatc tggaataaat gttgttgagg cggtttttgt
      481 taaactttca attaattgat ttatttgttt tttatcttgt ttaggcaagt cataattttc
      541 cgaaaaatag caatctttaa tatgagaaag cgatttcaag taagcacgta ataaggctcg
      601 ttgcatgtca tggttcaata tcccgtcttt actctctatc caccttgctt ttagcttgga
      661 gctaccattt ccaataattg ttgaagcaag attaattcct aatcctgcca tcactgaagc
      721 accaacagtt ccaccaatca aatatcccgc agtagtaacg gagccaactg ccaacaattc
      781 tgtcaaatct aatatcaaac ttttacttat cattgtctaa aaatgtttgc tttatgtttt
      841 tcttctcata ttgcttggcc tacacctgtc ctccttgtgt catattcgta aaagtcacat
      901 ttcaattcta aatatcttgt caattcatcg atgtttattt ggtgctcaga aaagtctttc
      961 gggtgtatga caatatttag tttatcgcaa aatcttcttt cagtttctgt cgtggcggaa
     1021 acaaaagagt tgataattga tttaataact ttttctcttg aatcaattaa ccgtccacgt
     1081 ccagtcccta ttattggaat attgatttgt ccagattctc cattagtcga aacataagac
     1141 catagtgaac ctaaactagt ttgtatgttg tcaaaatttg tttcagccac accattattg
     1201 ttcatgtcag atatggctga taaaaatgca tatttatcat tttcaagttt aagttttaga
     1261 actttcccaa tgtcataccg atatttatta cccctatttt tttgtggaag ttgagtagtc
     1321 ggtggctcac aggctagagc aattgtcaag tcttggtcaa gataacgagg ctctttaaaa
     1381 tatttgatag taaattgtcc ttgtgtgctt gtgttagcta ttaagtcacc ttcaaatgaa
     1441 gtgtcataag aagtattgat tggaataact aaatgccctt tgtagtcaaa caaattaccg
     1501 atagaaattt gaattcttat gtcacgttca tttagcttgt aacaaaattc cgtttttggc
     1561 cagttctcat atattaccca aataccacct aaaactaaca gccatagcca atgagctttg
     1621 aaaaagtcgg aaagcccctg tgaaaaataa cttgtcggct caattagtaa ccagataatg
     1681 cctaatgttc ctaaagctgc attaaaagtc ttcttaatgg agaatatata tttccaatag
     1741 ttcttcgtaa atattgtcag aaatcttttt atcatagtcc aagttggctg taaacagttg
     1801 gtttataaga aaatggtcca gacttggctt cccttttaag attttgagaa atctcacgcc
     1861 actcggacat agctttttca ataatttttt gattgaagct tatgtgaaga acaagtttgt
     1921 cctttagtat aacaggacac ctattgtcgt ctaattctct tttgccattt agattaattg
     1981 caactattgg aatttcttgt ttgagagctt gttccatttc ccagcgaaca aattttgtaa
     2041 ggaatttcgt ttgctcacca actagcacaa caaacaaatt agagctattc agtctttcta
     2101 gaagttgtcg ttttattgat tcttctttac ttgagtcacg agcataattt atttcatgtg
     2161 catcatagaa gttgaagtcc ttgaagaagt cattttgatt atgcttccat gctttcatca
     2221 ggtagaaata atgaatgtca ttatcaccgt caaagcataa atacgttttg tttctgtatg
     2281 ccattgttgt ctttttttat tatcggttta ctgtcgttta aaatgccaca taacggacgg
     2341 aactatatgc agttggggat tgcgggcttc gttcctgtcg aaccccgatg ccgttgttgc
     2401 gggctgagaa ctatcttgtc cgcaccgaaa ccccaattga catatagttt ttgttagcaa
     2461 ccgtagtttt tgtcattcgt taatttttat ccaatatgag cctccgtgtt tttcaacttc
     2521 ttttagatta acatgattcc atattggcga aattaaatca gataattctt tgtcttcaaa
     2581 tatgaaataa tttggaaaaa tcgtatcata tttcttagga tttgccagta aatacattgc
     2641 taatccatat tgctctgaat agaatctatc acacataaac tgcggataat cataaggcaa
     2701 ataaatatca tgaatgtgaa caataacacc ttttttcaac cttggtaaaa tctcaagata
     2761 gaaaaccatc gaatctgaat tcggcagaat cctatgagaa ttatcaacga atagtatgtc
     2821 gttttcattt agctcgtcca agatatcaaa atcaatattc tcaaaaggct ttctaattac
     2881 tttgtttgcc aaattgtcaa tttcggctcg cggcattggg tcgattgaaa taatttcagt
     2941 atctaaattc tgctcagtct ttgttttata agcaactttt gtcgagtttc ccgacccaat
     3001 ctcaatgtat ttttttggtt tgaactctgt cagtaaaatg taaatgccaa taatatcaag
     3061 acctggcaaa aaaccgttat tccatgtcgg ttttgttgaa tcagtctcat tacttgaatc
     3121 tttaattgtc caaatgaaat ccttaagcaa caatgattta tcaattaatt gcttgtaata
     3181 atctcggttt gagtcaataa ttttatataa atctttatga ggtggtttcc caaatccata
     3241 tcttggttta aaatcaactt tgtactcaag gaataaattc tgaaattttg ggttcaaaaa
     3301 tctgtaactc tgttttatca tattctaaaa tttaatctgt tgctgcgccg ttttactatg
     3361 gttgctaacg gctgaggcta aaagctggcg ggcatttcgg agcacttcac tgtcaagttg
     3421 cgatgaagtt tattagttgt gttgcccttc aaattaccac tttccgcccg cttgatttta
     3481 gcctttgtta gcgtttcgtg ctttctattc ataagtatct gtctgttata tattcaatgt
     3541 acttctcatt tgtgtcgtag tagttatcgg ccaagaaggt tattaattcc tgataactaa
     3601 ttgaactaaa ttttatgtcg tctgcttttg tatattctga aaacttgtca atttcgttgc
     3661 gatgtatata accttcttcg cctaatacgt cataccacaa atacaaaagt cgaaaagatt
     3721 ttttcccgta tttattttta agtcctaaaa tatgcttgat taattgtgca ggatgcaaaa
     3781 acttaaaatc actgtcgtct gggcttattg cttttgcaag ttcatataat tttggaatat
     3841 ctttccataa tggtaaaata tcgttgttga aatattttgg gtcaactccg ctgtgtccgt
     3901 gatttgaata cgcttctgaa tacttgcatt ctatggcaaa gactttatat tgtgctttat
     3961 cattgttttc aattacaacg tcaaggttag ggcaaaaagg aaaggcttta ctaataataa
     4021 atttttgttc aaaatttatg tctgtttgag ttttattttg ttttgcacaa agtccgcaag
     4081 cgtgagaaat attgtctatt ttaccagtcg ttttccaata atgaaaaagg tttacaccga
     4141 gtgcagacga tgaatgaaca gcattcattt ttgctggaga attattctta tttaaaattt
     4201 cacctccatc acctttagaa aagtcagcaa taacatcggc tgccaatggt tcaaataggt
     4261 tttcctcaag cttttttgtg tatgctggaa aacctttatc caccttgcta ccaatgagtg
     4321 aaatattttt cctttttgcc cattgaactt gctttgccaa tatgtattca aatcctttca
     4381 taagtcagat taatatttat taagattgtc catattaact tcgggcggaa ttggcgaaac
     4441 ttttttagca gatgctcttg aggcgaaaag aacaattaaa tagattacaa ggcaaatacc
     4501 tgcggaaatt aatagaactt tccattgcaa aagcgtgtct ggattttcgt attctgcacc
     4561 tgttcgggct gaaaagagca tcttaaccaa gtcaactgga aatcgaatta tggcatttac
     4621 accataagaa aaatcagaaa attcttttga tatgaaaatc cctgttgtaa ttaaccacgc
     4681 tacacttgaa attagaaaac tgtaaaacct tgtgtaaatc tttctgaact tcaaccatct
     4741 tttgttacca gaaagcaaat atgtcgtgat ttcggtaatt agtgatgtac cgatt
//
LOCUS       DAOTCW010000162         4719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_13883_length_4719_cov_49.234777, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000162 DAOTCW010000000
VERSION     DAOTCW010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13883_length_4719_cov_49.234777"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="P5335_10460"
     CDS             complement(<1..134)
                     /locus_tag="P5335_10460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRZ75343.1"
                     /translation="MSALVSIITPTYNSAKYIAETILSVQNQTYSTWEMIIIDDGSFD
                     "
     gene            complement(134..1501)
                     /locus_tag="P5335_10465"
     CDS             complement(134..1501)
                     /locus_tag="P5335_10465"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HRZ75344.1"
                     /translation="MVANQKGRYSKFIRPISVSLDLLVITLLAFFFFEELNLDFFLFE
                     TYQVVCWILIAFFVGFYEVYRFTTPVEILSKILKQSIVFLLVVIAYFPFAKDSLFSGK
                     AVALFMTSSFILIVFIKYLLFYYLKKYRIVTGSNFRNAVIIGYTEEAINLKDLFEKRN
                     DYGYRFFGFFSDKKSNENIKGKVSEIKEFVINNKIDEIYCSLNEITNEQLKDLVEFAD
                     ENNKTIKFIPDTKQIFSKNLKIDYYEMFPVLSLQRTLLHEPITKTFKRIFDIIFSLCV
                     IVFLLSWLVPLLALLIKLESKGPVFFKQGRPGLDEEEFFCYKFRSMQMNHTTEKEASK
                     NDPRVTKIGRFLRKTSLDELPQFFNVLLGDMSVVGPRPHLWSQNRVYGNRVKKYMVRH
                     YVKPGITGLAQVKGFRGEIETDEDMINRIKLDVFYIENWSFILDIKIIFQTVINIFKG
                     EEKAY"
     gene            complement(1503..2486)
                     /locus_tag="P5335_10470"
     CDS             complement(1503..2486)
                     /locus_tag="P5335_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRZ75345.1"
                     /translation="MKRILITGAAGFLGSHLCDRFIAEGYYVIGMDNLITGDLKNIQH
                     LFKLENFEFYHHDVTKFVFVPGNLDYILHFASPASPIDYLKIPIQTLKVGSLGTHNLL
                     GLARVKNARILIASTSEVYGDPLIHPQTEEYYGNVNTIGPRGVYDEAKRFQESITMAY
                     HTFHGLETRIVRIFNTYGPRMRLNDGRVIPAFIGQALRGEDLTIFGDGMQTRSFCYVD
                     DQVEGIYRLLLSDYDLPVNIGNPDEITIKDFADEIIKLTGTTQKVVYHPLPINDPLQR
                     QPDITKARAILGWEPKVSREEGMKITFDYFKTLSTEELLKEEHKDFSGFIH"
     gene            complement(2483..>3043)
                     /locus_tag="P5335_10475"
     CDS             complement(2483..>3043)
                     /locus_tag="P5335_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRZ75346.1"
                     /translation="EFQSNTEKSLSSTVIDKDLVGVNVLSVYEAWTNEKFTPNDFFPG
                     TAFRVYQARMFFELFQEESVFWKGYGLNASFKKLEEKAFKYDVFRGNETQEGYQTKNF
                     HNQYIQNFAELGFFGFLILVIMLLFTLKKTIQNKDFIQIAFAVLMISLFLTESFLWRQ
                     RGVMFFTLLYCLFNTEYYFAPKKNVL"
     assembly_gap    3044..3143
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3144..3643)
                     /locus_tag="P5335_10480"
     CDS             complement(<3144..3643)
                     /locus_tag="P5335_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75347.1"
                     /translation="MSNVWLDIKQENKQHFSFIPFLLVLCTIPMAYGINNVFLAIFSL
                     SVLIKYSSSTFRLQKWLLPAVALFLLMLLSYFWSIDKDATLKAIPKEISLLLIPILFM
                     VMKPFSKEQGQKIIKYYSYAMVLYVTFYLLKAMVRFILTNDTNVFFYHELVTKEVNAI
                     HVSVYVA"
     gene            complement(3606..4712)
                     /locus_tag="P5335_10485"
     CDS             complement(3606..4712)
                     /locus_tag="P5335_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HRZ75348.1"
                     /translation="MKIAILGTRGIPNYYGGFEQFAEFFSVYLAQKGHEVYCYNSHNH
                     KFQENNFKGVHIIHQHDPEYKYGTFGQFIYDYNCILDSRKRDFDIILQLGYTSNSVWF
                     FMLPKKAIIITNMDGIEWKRSKYNRPVQQFLRFAERLAAISSDYLVSDSIGIQTFLKN
                     RYKKESTYIAYGAYPFTNPNESILTEYQVEKEKFNMIMARFEPENNFEMVLDGVVKSE
                     DKTPILVIGNHNTKYGEYLKSRYKNQFNIKFLGAIYNLEHLNNLRYFSKLYFHGHSVG
                     GTNPSLLEAMASKALVLAHNNDFNRGILKENSYYFSNAEEVKNLLLKIKKNDNLQFIQ
                     NNFEAIVNKFNWEKINGEYLQLFEQCMARHQAGK"
BASE COUNT         1594 a          791 c          672 g         1562 t
ORIGIN      
        1 tcgtcaaatg atccatcatc aataataatc atttcccaag ttgaataggt ctgattttga
       61 acggataaaa tcgtttctgc aatatattta gctgaattat aggtgggtgt aataattgaa
      121 actaaagccg acattaatac gctttttctt cacccttgaa gatatttatg accgtttgaa
      181 aaataatttt tatgtctaaa ataaatgacc agttttcaat ataaaagaca tctaatttaa
      241 tacgattaat catgtcttca tctgtctcta tttctcctct aaaacctttt acttgagcca
      301 ggccggtaat tcccggtttg acatagtggc gaaccatata ttttttaacg cgatttccat
      361 aaactcggtt ttgggaccat aaatgaggtc tgggacctac aactgacata tcacccaaca
      421 acacattgaa aaactgaggt aactcgtcta agcttgtttt tcttaaaaat cgaccaattt
      481 ttgttactct tggatcattt tttgatgctt ctttttcagt cgtatgattc atttgcatgg
      541 agcgaaattt ataacaaaaa aactcttctt cgtctaaacc cggtcttcct tgtttaaaaa
      601 aaacaggtcc ttttgattca agttttatta acaatgctaa aagcgggacc aaccatgata
      661 aaagaaaaac gattacacat aaagaaaaaa taatgtcaaa tatccttttg aatgttttgg
      721 taattggttc atgtagcaac gttcgttgaa gcgataaaac cggaaacatt tcgtaataat
      781 cgattttcag gtttttagaa aaaatctgtt ttgtatctgg gataaacttg atggttttgt
      841 tgttctcatc agcaaattca accaaatctt ttaattgctc atttgtaatt tcattcaatg
      901 aacaatagat ttcgtcaatt ttgttgttta ttacaaactc tttaatctct gaaacttttc
      961 ctttaatgtt ttcattgctt tttttatctg agaaaaaacc aaaaaagcgg taaccataat
     1021 catttctttt ttcaaaaaga tctttcaaat taattgcttc ttcagtatag ccaattataa
     1081 cggcatttct aaaattactt ccggttacaa ttctatattt ttttagataa taaaacaata
     1141 aatatttaat aaatacaatc aaaataaaac ttgaagtcat aaacaatgct acagcttttc
     1201 cgctaaacaa agaatctttt gcaaaaggaa aataggcaat cacaaccaat aggaatacaa
     1261 tcgattgttt taaaatttta gataaaattt caaccggcgt agtaaatcga tatacttcat
     1321 aaaaaccgac aaaaaaagct attaaaatcc aacatactac ttgatatgtt tcaaaaagaa
     1381 aaaaatcaag atttaattct tcaaaaaaga aaaaggctaa caatgtaatg accaataagt
     1441 ctaatgaaac acttatcggc ctaataaatt tagaatatct tcctttttga ttagctacca
     1501 tattaatgaa taaaccccga aaaatccttg tgctcttctt ttaacaactc ttcagtagaa
     1561 agtgttttaa aataatcaaa ggttattttc attccttcct ctcttgaaac ttttggttcc
     1621 catcctaaaa ttgctcttgc tttggttata tccggttgtc gttgtaacgg atcgttaatt
     1681 ggtaaaggat gataaactac tttttgagtt gttccggtta atttaattat ttcatccgca
     1741 aaatctttaa tcgtgatttc atccggattt cctatattaa ccggtaaatc ataatcagat
     1801 aataaaagac gataaatacc ttccacttga tcatctacat aacagaaaga acgcgtttgc
     1861 atgccatcac caaaaatagt taaatcttct cctcttaatg cttgtccgat aaaagcagga
     1921 attacacgac catcatttaa tcgcattctt ggtccgtaag tattaaatat tctaacaatt
     1981 ctggtttcta aaccatgaaa agtatgataa gccattgtaa tggattcttg aaaacgtttt
     2041 gcttcatcat aaacaccacg cggaccaatg gtatttacat ttccgtaata ttcttccgtt
     2101 tgtggatgaa tcaatgggtc tccatatact tcagaggttg atgcaattaa aattctggca
     2161 ttttttactc ttgccaaacc gagtaaatta tgcgttccta gcgaacctac tttcaatgtt
     2221 tgaatgggaa tttttaaata atcgatagga cttgccggcg aagcaaaatg tagaatataa
     2281 tctaagttgc cgggtacaaa tacaaatttg gttacatcat ggtgataaaa ctcaaaattt
     2341 tccagtttaa ataaatgctg aatatttttt aaatcaccgg taattaggtt atccatccca
     2401 atcacataat aaccttcagc aataaatcga tcacatagat gcgagcctaa aaatccggcg
     2461 gcacctgtta ttaaaattct tttcataaaa catttttttt aggggcaaaa taatattcgg
     2521 tattaaacaa acagtaaagt aatgtaaaaa acattacccc tcgttgtctc cataagaaag
     2581 attctgtcaa aaataaactt atcattagaa ctgcaaaagc aatttgtata aaatctttgt
     2641 tctgtatagt ttttttaagg gtaaaaagca acattatgac taaaatcagg aaaccaaaaa
     2701 atcccaattc agcaaaattt tgaatatatt gattatgaaa gttttttgtt tggtaacctt
     2761 cttgggtttc atttcctcta aaaacatcgt atttaaaggc tttttcttct aattttttaa
     2821 aagaggcatt gagtccgtat cctttccaaa aaacagattc ttcctgaaat aattcgaaaa
     2881 acatcctagc ttgatagacc cgaaaagccg tgccggggaa aaaatcattg ggtgtgaact
     2941 tttcattggt ccacgcttca taaacactta gcacattcac acccaccaaa tctttatcaa
     3001 ttaccgtaga actcaaactt ttttccgtat tggattgaaa ttcnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnngcaacat agacggaaac atgaatggca ttgacttctt
     3181 tggttaccaa ttcatgataa aaaaagacat ttgtatcatt cgttaaaata aatcgaacca
     3241 tcgctttaag caaataaaaa gtgacatata aaaccatcgc ataactataa tatttgatga
     3301 ttttttgccc ttgttccttt gaaaatggtt tcatcaccat aaacaaaatt ggaatcaaca
     3361 gtaaactgat ttcctttgga attgctttta acgtggcatc tttatcaatg ctccaaaagt
     3421 aagaaagcag cattaataaa aataaagcca cagcaggtaa aagccatttt tgaagtcgaa
     3481 atgttgaaga ggaatattta attaaaacgg aaagcgagaa tatcgctaaa aaaacattgt
     3541 taattccata ggccatcgga atagtacaca acaccaacaa aaagggaata aaagaaaaat
     3601 gttgtttatt ttcctgcttg atgtctagcc atacattgct caaaaagttg aagatactcc
     3661 ccatttattt tctcccagtt aaatttgttt acaatggctt caaagttatt ttgaatgaat
     3721 tgcaagttat catttttttt gattttgagc agtaaatttt ttacttcttc tgcattggaa
     3781 aaataatagc tgttttcttt taaaatacca cgattaaaat cattgttgtg ggctaaaact
     3841 aaagctttgg aggccattgc ttccaacaat gaaggattgg ttccgccaac tgaatgacca
     3901 tggaaatata atttagaaaa atagcgaaga ttatttaaat gttcaaggtt ataaatagct
     3961 cctaaaaatt tgatgttaaa ttggttttta tatcttgatt tcaggtattc tccatatttt
     4021 gtattgtgat taccaatgac taaaattggg gttttgtctt cacttttaac cacaccatcc
     4081 aacaccattt caaaattatt ttcaggttca aaacgagcca taatcatgtt gaatttttct
     4141 ttttcaactt ggtattccgt tagaattgat tcgtttggat tagtgaaagg ataagctccg
     4201 taagcgatat aagtagactc ttttttatat ctatttttca aaaaagtttg aattccaatg
     4261 gaatctgaaa ccaaataatc actgctgatt gccgctaacc gttcagcaaa acgcaaaaat
     4321 tgttgcaccg gacggttata ttttgaacgt ttccattcga ttccatccat attggtaata
     4381 ataattgctt ttttaggcag cataaaaaac caaaccgaat tactggtgta acccaattgc
     4441 agaatgatat caaaatctct tttgcgggag tctaaaatac aattgtaatc gtagataaat
     4501 tgaccaaacg ttccatattt atattccgga tcgtgttggt gaatgatatg aacacctttg
     4561 aagttatttt cctgaaattt atgattgtgc gaattataac aatatacctc atgccctttt
     4621 tgagccaggt aaacggaaaa aaattcggca aattgttcga aaccaccgta ataatttgga
     4681 ataccgcggg ttccgagaat ggctattttc attcttttt
//
LOCUS       DAOTCW010000163         4641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_14207_length_4641_cov_52.898386, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000163 DAOTCW010000000
VERSION     DAOTCW010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14207_length_4641_cov_52.898386"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="P5335_10490"
     CDS             complement(<1..1289)
                     /locus_tag="P5335_10490"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRZ75349.1"
                     /translation="MKRITLVILLFLLTGVGYSQLNENFESGIPATWAVFNNGLGTNT
                     WTTTTAVTTPPTVCEGTTAAFMNARQNIGAGNTSQNWLVTPQVLVPANGQLFFQTRST
                     ISGPNDTTYELRLSTTSQTNTASFITIQSWTEAELNATFNICEEKQVNLAGFAGTNVY
                     LAFVIVMNQPAAGLTGDRWIVDDVRLVEQCINPENITVTGITQTSAIINWDNPGGATQ
                     FEVSVVPAPGTPVAGTIVGPSPYLAEGLSATTQYQVYVRAKCEFSNSEWVGPVNFITT
                     TPGTACNSAIQVPALPYSTTDNTANYQDDIDGSPGASGCGTTGNFLNGNNVYYSYTAT
                     ATGVINLTMTPTGNNSGMFVYNSCTNVGVSCIAGTVSVAGAPLVIDLPVVDGQTYYIV
                     ISTNGNPNTIPYTLSIQVVNCAPPTNLGAVGGQTSAI"
     gene            1479..2699
                     /gene="hflX"
                     /locus_tag="P5335_10495"
     CDS             1479..2699
                     /gene="hflX"
                     /locus_tag="P5335_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRZ75350.1"
                     /translation="MLEKEKINFEKTIVVGIVTKEQDETKLNEYLDELEFLTFTAGGE
                     VVKRFWQKLEKPHPKTFLGTGKIEEIHRYIVENEISSVIFDDELSPAQQKNITKILDC
                     KVLDRTNLILDIFAQRAETSYARTQVELAQCQYLLPRLSGMWTHLERQRGGIGMRGPG
                     ETEIETDRRIVRDRIALLKEKIKTIDKQMSVQRGNRGAMVRVALVGYTNVGKSTLMNA
                     IGKSDVFVENKLFATLDTTVRKVVIRNLPFLLSDTVGFIRKLPTQLVESFKSTLDEVR
                     EADLLLHVVDISHPDFEDHIASVNQILQDIKSADKPIIMVFNKIDAYKPVHLADDDLV
                     TERTSKHYSLDEWKKTWMSNVGKNNALFISATNKENFEEFKEIVYEAVREIHITRFPY
                     NKFLYPDYKDAVED"
     gene            complement(2762..3682)
                     /locus_tag="P5335_10500"
     CDS             complement(2762..3682)
                     /locus_tag="P5335_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HRZ75351.1"
                     /translation="MKKIILALSMFAFFSIQAQKEVVKEIEKVTKAVEDTTRTHGWTR
                     NGEITFLLNQSSFSNWAAGGEDNLSGNIGINYGFNYKSVDWSWDNRIIASYGLVKTQN
                     AAFEKKTDDRLEFNSLAGKKAKGYWYYSFILNFRTQFTQGYEYSTDPNGKEDRKEFTN
                     FMSPGYLTFGPGLLWKKSDNFKFNLAPLTSKMTFVDNSYTSGVGYVDGTYFGVDANKS
                     FRYELGFYASGYYKFNLFENVSAENFLTLYSNYLEDPQNVDIDYQLNLVMTINKFLST
                     NLSFQTIYDDNAFRGFQIREVFGLGVNYKF"
     gene            complement(3773..4279)
                     /locus_tag="P5335_10505"
     CDS             complement(3773..4279)
                     /locus_tag="P5335_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HRZ75352.1"
                     /translation="MDEIINKVANSALEVFDLEDYYPSGIRTQIDIALWLEEGFLLKE
                     KDFREALKNYDWSQYQNHFVAIHCSTEAIIPAWATILVTVYLQPFAKKIILGKKDEID
                     TLLYLEILNQMDYSIYLNKPVILKGCSKKPVPESAYVLAVQHLQLFAKSIMFGEACSA
                     VPLFKKGK"
     gene            complement(4314..4637)
                     /locus_tag="P5335_10510"
     CDS             complement(4314..4637)
                     /locus_tag="P5335_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HRZ75353.1"
                     /translation="MEEFKDDINLGENVVKVLKGIYDPEIPVDIYELGLIYDVMINED
                     NDVKVLMTLTSPNCPVAETLPQEVQEKVKSLDAVKSCEVEITFDPPWTKDLMSEEAKL
                     ELGML"
BASE COUNT         1483 a          822 c          809 g         1527 t
ORIGIN      
        1 aaaatggcac tggtttgacc acctaccgct cctaaattgg ttggaggtgc acagtttaca
       61 acttgaatag ataaagtata tggaatcgtg ttaggattac cattggtaga aattacaata
      121 taataggttt gaccatccac aaccggtaaa tcaataacca gaggagcacc ggcaacactg
      181 acagtaccag cgatacaact tactccaaca tttgtacaac tgttgtaaac aaacataccc
      241 gagttgtttc ctgttggcgt cattgttaaa ttaattacac cggtagctgt ggcggtataa
      301 ctataataaa cgttgtttcc gtttaaaaaa tttcctgttg ttccacaacc ggatgctcca
      361 ggacttccat caatatcatc ttgataattt gctgtgttat ccgtggttga atatggtaat
      421 gctggtactt gtatagccga attacaagct gttcccgggg ttgtagtgat aaaattcacc
      481 ggtcctaccc attcactatt gctaaattca cattttgctc taacatatac ttggtattgt
      541 gtagtagccg ataatccttc tgctaaataa ggacttggcc caacgattgt acctgcaacc
      601 ggagtaccag gagccggaac aactgaaact tcgaattgag tagcacctcc gggattatcc
      661 caatttataa tagcactggt ttgtgtaatc ccagtaacag taatgttttc aggattaata
      721 cattgttcta ctaaacgtac atcatcaaca atccaacggt caccggtaag tccggcagca
      781 ggttggttca tcacgatcac aaaagcaaga taaacatttg taccagcgaa accggctaag
      841 tttacttgtt tctcctcaca aatattaaac gtagcattaa gttctgcctc tgtccatgac
      901 tggatagtaa taaaacttgc cgtgttagtt tgtgaagttg tggataaacg caattcatac
      961 gttgtatcgt ttggcccact aatggttgat ctcgtttgaa agaacaactg tccgtttgcc
     1021 ggtactagta cctgaggggt aactaaccag ttctgagacg tgttgcccgc tccaatgttt
     1081 tgacgagcat tcataaaggc tgctgttgta ccttcacata ccgttggcgg agtagtaacc
     1141 gcagtggttg tggtccatgt gttggtacct aaaccattgt taaatactgc ccatgttgcc
     1201 ggaataccgc tttcaaagtt ctcattgagc tgcgaatatc ccactcccgt cagtaaaaac
     1261 aatagtataa ctaatgtaat tcttttcata aaatgaaagt ttaggttgtt ttgattaatt
     1321 ttttgtaggt ttagaacgtc aaatttaaca aaatttaaga attggataac ttttttttat
     1381 attttaacta aattcatcct ttatattatt tgttttaatg gttaaaattc gatttaaacc
     1441 ggcttgaatt gcctaatttt gcaagacatt tataggatat gttagaaaaa gaaaaaataa
     1501 attttgagaa aacaattgtt gtaggaattg ttacgaaaga gcaagatgaa actaaattaa
     1561 acgagtattt agatgaatta gagttcctta cttttactgc cggtggtgaa gtagtaaagc
     1621 gtttttggca aaaattagaa aaacctcatc cgaaaacatt tttaggaacc ggtaaaattg
     1681 aagaaattca tcgatacatt gttgaaaatg aaatttcatc cgtcattttt gatgatgaat
     1741 tgtcgcctgc acaacaaaaa aatatcacta aaatcttaga ctgtaaagtt ttagacagaa
     1801 ctaacttgat tcttgacatt tttgctcaac gggcagagac ttcttatgca agaacacagg
     1861 tggaacttgc tcaatgtcaa tatttgttgc ctcggttatc gggaatgtgg actcacttgg
     1921 agcgtcaacg aggtggaatc gggatgagag gaccgggaga gacagaaatt gaaaccgacc
     1981 gtcgaattgt gcgtgaccga attgccttgt tgaaagaaaa aatcaaaacc attgacaaac
     2041 aaatgtcggt acaacgcgga aatcgcggag ctatggttcg tgtcgcttta gtaggttata
     2101 ccaacgtggg aaaatcaact ttgatgaatg caatcggaaa aagcgatgtt tttgtagaaa
     2161 ataaattatt tgcaacacta gacacaacgg ttcgaaaagt ggtgattcga aacttgcctt
     2221 ttctattatc ggatacggtt gggtttattc gaaaattacc cactcaattg gtcgaatcat
     2281 ttaaaagtac cttggatgaa gttcgtgaag ccgatttgtt acttcatgtg gttgatattt
     2341 cgcatccgga ttttgaagat catattgcct ctgtaaatca aattttgcag gacattaaaa
     2401 gtgctgacaa acctatcatc atggttttca ataaaataga tgcatacaag cctgttcatt
     2461 tagcagatga cgatttagtt accgaaagaa cctctaaaca ttattcttta gatgaatgga
     2521 aaaaaacctg gatgagtaat gtaggtaaaa ataatgcctt gtttatttct gctacgaaca
     2581 aagaaaattt tgaagaattc aaggaaattg tatatgaagc ggttcgagaa attcacatca
     2641 cacgtttccc ctacaataaa ttcttgtatc cggattacaa agatgccgtt gaagattaaa
     2701 atctagataa aagaaaatgg ctctccaatt ttgagagcca tttttttatg tttaactaaa
     2761 attagaattt ataattaacc cctaacccaa aaacttcacg aatttgaaat cctctgaacg
     2821 cattatcatc ataaatggtt tgaaaggaaa gattggttga taaaaattta ttgatagtca
     2881 ttaccaaatt taattgataa tcaatgtcaa cattttgtgg atcttctaaa taattggaat
     2941 acaaggttaa aaagttttcg gcagatacat tttcaaataa gttaaatttg taatatcctg
     3001 aggcataaaa acctagttca taacgaaaac ttttattggc atcaactcca aaataagttc
     3061 cgtcaacata tcccactcca gatgtataac tgttgtctac aaaagtcatt ttagaagtaa
     3121 gtggagctaa attgaattta aaattatcac tctttttcca taacaaaccc ggaccaaaag
     3181 ttaaataacc cggcgacata aagttggtaa attcttttct atcttcttta ccatttggat
     3241 cagtagaata ctcataacct tgtgtaaact gcgttctaaa gtttaagata aatgaataat
     3301 accaatatcc ttttgctttt tttccggcta aggaattaaa ttccaatcgg tcatctgttt
     3361 tcttttcaaa agcagcattt tgtgttttta ctaaaccata ggaagcaatt attctgttgt
     3421 cccacgacca gtcaacactt ttataattaa aaccatagtt aattcctata tttccggaaa
     3481 gattgtcttc acctccggca gcccaattgc taaaactgga ttgatttaat aggaaagtga
     3541 tttctccatt tctagtccat ccatgagttc gtgtggtgtc ttcaaccgct ttggttactt
     3601 tttcaatttc tttcacaacc tctttttgag cttgtattga aaaaaaggca aacattgata
     3661 aagcaagaat aattttcttc attttttgtt aaatttacat tttgcgatgc aaagttaaca
     3721 tttgaaatga ttcatgcaaa taatataaca agctgataat aagaaaacta ttttattttc
     3781 cttttttgaa taatggaact gcagaacaag cctcaccaaa cataatgctt ttagcaaaaa
     3841 gttgtaaatg ttgaactgct aaaacataag ccgattccgg taccggtttt tttgagcaac
     3901 cttttaaaat aacaggtttg tttaaataga ttgaatagtc catttgattt aaaatttcaa
     3961 gatataataa agtatctatc tcgtcttttt ttcctaaaat tatttttttt gcaaacggct
     4021 gtagataaac agttaccaaa atagttgccc atgccggaat aatggcctct gttgaacaat
     4081 gaattgctac aaaatggttt tggtattgtg accaatcata gttttttaag gcctctcgga
     4141 agtctttttc tttcaacaaa aaaccctctt caagccatag agcaatatca atttgtgttc
     4201 gaattccgga aggataataa tcctctaaat cgaaaacttc gagtgcggaa ttggctactt
     4261 tgttgatgat ttcgtccatt tttaatgtcg tttatttaga ttggacaact attttatagc
     4321 attcccaatt ctaactttgc ttcttcactc attaagtctt tggtccatgg tggatcgaac
     4381 gtaatttcaa cttcgcatga ttttacagca tccaatgatt ttactttttc ttgcacttcc
     4441 tgaggtaatg tttccgcaac aggacaattt ggtgacgtta acgtcattaa tacttttaca
     4501 tcattgtctt cattaatcat tacatcgtaa attaatccaa gttcgtatat gtctacggga
     4561 atttcagggt cgtaaattcc tttaagtacc tttactacgt tttcacctaa gtttatgtcg
     4621 tctttaaatt cttccatttt a
//
LOCUS       DAOTCW010000164         4607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_14348_length_4607_cov_47.903120, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000164 DAOTCW010000000
VERSION     DAOTCW010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14348_length_4607_cov_47.903120"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="P5335_10515"
     CDS             complement(<1..602)
                     /locus_tag="P5335_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRZ75354.1"
                     /translation="MNKPAFSIYDASAGSGKTFTLVKEYLKIILTSSKEDAYKNILAI
                     TFTNKAVGEMKSRVVNTLSEFAQVNPNTKAQEVMAQIQAETQLSLEDIHIKSKAIIRN
                     IIHNYAAFDISTIDKFTHKVIRTFAHDLNLPSTFEVSLETENLLTEAIDALIAQAGDD
                     EMLTDLLVNFTLEKTDDDKSWDISRDIKEIGKLLSNENNR"
     gene            707..1315
                     /locus_tag="P5335_10520"
     CDS             707..1315
                     /locus_tag="P5335_10520"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRZ75355.1"
                     /translation="MSFELPKLPYAYDALEPHIDARTMEIHHTKHHAAYTNNLNAAIA
                     GTDMEGKTIENILINLDKTNAAVRNNGGGFYNHNLFWTVMTPDGGGEPTGELAEAIER
                     DFGTFAEFKAKFAKAGATQFGSGWAFLCVQKGGKLDICGTPNQDNPLMPGVGCGGTPI
                     LGMDVWEHAYYLHYQNRRPDYIEAFFNVINWSEVSRRYAIDK"
     gene            complement(1741..3639)
                     /locus_tag="P5335_10525"
     CDS             complement(1741..3639)
                     /locus_tag="P5335_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRZ75356.1"
                     /translation="MSDNLKHECGIALLRLKKPLQYYKDKYGTAFYGIQKMYLLMEKQ
                     HNRGQDGAGFASIKFDVEPGQRYISRVRSNQAQPIQDIFAQINERINEELSTHPEYQD
                     NVALQKEHIPYIGELFLGHVRYGTFGKNSIESVHPFLRQNNWMHRNLIVAGNFNMTNV
                     KELFDNLVELGQHPKELADTVTVMEKIGHFLDDEVTDLYQECKNNGLSKREASPVIAE
                     KLDIAKILRKASKNWDGGFAMAGLLGHGDAFVFRDPAGIRPVFFYEDEEIVVVASERP
                     VIQTVFNVPFEKVQELDPGKALIVKKNGDFSIEEILEPLEKKACSFERIYFSRGSDAE
                     IYKERKMLGKLILPAVLDSIESDTDNTVFSYIPNTAETSFYGMVEAAQDFLNQRKNKF
                     ILDNRKTLTKEKLEEILSVKIRTEKVAIKDAKLRTFITEDSSRDDLVAHVYDVTYGVI
                     KPSDNLVIIDDSIVRGTTLKKSIIRMMDRLNPKRIVIVSSAPQIRYPDCYGIDMAKLE
                     GLVAFRAVLELLKDTNQYHLVDSVYKKCKQQEKYKDGEIVNYVKEIYAPFTNQEISDK
                     IAIMLSSSDIKAEIKIIFQTVENLHVACPNHLGDWYFTGNYPTPGGNRVVNRAFMNFY
                     EGKDARAY"
     gene            complement(3771..>4607)
                     /locus_tag="P5335_10530"
     CDS             complement(3771..>4607)
                     /locus_tag="P5335_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRZ75357.1"
                     /translation="TFIGLSASYFDVDAAIVSVVGEDFPQEYLDLLSDRDIDISGIEI
                     VKGGKTFFWSGRYHNDLNSRDTLETQLNVLADFQPKVPTEYKDAAVVMLGNLHPMVQI
                     SVLNQMTEKPKLVVLDTMNFWMDCALPEFHQVMKRVDVITINDEEARQLSGEYSLVKA
                     AAKIHTMGPKYVVIKKGEHGALLFEGKEIFFAPALPLEEVFDPTGAGDTFAGGFSGYI
                     AQSENISFENMKNGIIYGSNLASFCVEKFGTERMVDLEKEEVYDRLKQFRALTQFDIE
                     IE"
BASE COUNT         1444 a          860 c          754 g         1549 t
ORIGIN      
        1 cttcgattat tttcattgga taatagtttg ccaatctctt taatatcacg ggaaatatcc
       61 caagatttat catcatctgt tttttctaaa gtaaaattta ctaataaatc agtaagcatc
      121 tcgtcgtcac cggcttgggc gattaatgca tcgatggctt cagtaagtaa attttcagtt
      181 tctaaggata cttcaaatgt tgagggtaaa ttcaaatcat gtgcaaaggt tcgtatgact
      241 ttatgtgtaa acttatcaat cgtagaaatg tcaaaagcag cataattgtg aataatatta
      301 cgaattattg cttttgattt gatatgaata tcctccaatg aaagttgggt ttccgcttga
      361 atttgagcca ttacctcctg agcttttgtg ttcggattga cttgagcaaa ctctgaaaga
      421 gtattaacta cccttgattt catttcaccc acagctttgt tggtaaaagt aattgctaaa
      481 atatttttat aagcatcttc tttacttgag gtaagaataa tttttaaata ttctttgact
      541 aaagtaaatg ttttacctga accggcagag gcgtcgtaga ttgaaaaggc tggtttattc
      601 aaaactataa tttaaattaa taaaactata actaaataga atttcttatt ccaaagttaa
      661 ttgattaaat ttgatgcaac tattgtcgaa cataaaaatt tatactatgt catttgaatt
      721 accaaaatta ccttatgcat atgatgcatt agaacctcat attgatgccc gtacgatgga
      781 aatccatcat accaaacacc atgctgctta taccaacaat ctaaatgctg ctattgcagg
      841 aactgacatg gagggaaaaa ctattgaaaa catattaatc aacttagata aaacaaatgc
      901 tgccgttcgc aataacggag gtggatttta caaccataac ttattttgga ctgttatgac
      961 tccagatgga ggtggcgaac caactggtga attagcagag gctatcgaga gagattttgg
     1021 tacttttgcg gaattcaaag ccaaatttgc aaaagccggg gcaacacaat ttggttcggg
     1081 ttgggctttt ttatgtgtgc aaaaaggagg taaactagat atctgcggaa caccaaatca
     1141 agataatccg ctaatgcccg gagttggttg tggtggaaca ccaattttag gaatggatgt
     1201 ttgggagcac gcatactacc ttcactacca aaacagaaga ccggattata ttgaagcttt
     1261 ttttaacgtt atcaattggt cagaagtttc cagaagatat gctatagata agtaaaaaag
     1321 aataactttc aattttataa aagcgataca ttttgtgtcg ctttttttat aaaataaaaa
     1381 ggtggaattt cttccaccct tttgccccaa atctaccata aacttaaccg tgcgtactat
     1441 ggtaataaca aatatacaac gctttattaa cagaaactat tgtattggaa tctaaatttt
     1501 aacgtttggg gttttaaggg tgtatgatgc ttttttggag gttattgaca actctttgaa
     1561 ggtggtaaaa ctatgtaaaa taaaaaaggt ggaattgctt ccaccctttt tgccccaaat
     1621 ctaccataaa cttaacctac taatgctatg gtgaaacaaa tgtataataa gagtattttg
     1681 ttaacaaaaa aattagatga aatgcaccaa aagtcgttaa aacgcataaa ttggcgtctt
     1741 ttagtaggct ctggcgtctt ttccttcata gaaattcata aaagctctgt tcacaacacg
     1801 attaccacca ggggttggat agttcccggt aaaataccaa tctcctaaat gattcggaca
     1861 agcaacatgt aaattttcaa ctgtttgaaa tataatttta atctccgctt tgatgtcaga
     1921 agaacttaac atgattgcta ttttatctga tatttcctga ttagtaaacg gtgcataaat
     1981 ttctttcaca tagttaacaa tttcgccgtc tttgtacttt tcctgttgtt tacatttttt
     2041 ataaacactg tctactaaat gatattgatt tgtgtctttt aataattcca aaacagctct
     2101 gaatgcaacc aacccttcta attttgccat atcaattcca taacagtccg ggtagcgaat
     2161 ttgtggtgct gatgaaacaa tcacaatccg ctttggattc aaccgatcca tcattcgaat
     2221 gatgcttttc tttaaagttg taccgcgaac gatgctgtca tctatgatta ctaaattatc
     2281 acttggttta atcacgccat aggttacatc ataaacgtga gcaactaaat catctctact
     2341 gctgtcttcc gtaataaaag ttcgaagttt agcatctttg atagctactt tttctgttct
     2401 gatttttaca gaaagtattt cttccaactt ttcctttgtc aacgttttgc gattatctaa
     2461 aataaattta ttttttcgtt gatttaaaaa atcctgtgca gcttcaacca ttccataaaa
     2521 agaagtttcc gctgtattcg gaatgtagga gaacaccgta ttgtctgtat cgctttcaat
     2581 ggaatccaaa acagcgggca ggattaattt cccgagcatt ttacgttcct tataaatttc
     2641 agcatcactc cctctggaaa aataaatacg ttcaaaggaa caagctttct tttctaatgg
     2701 ttcgagtatt tcttcaatag aaaaatcacc gtttttctta acaattaagg ctttccctgg
     2761 gtctaattcc tgcacttttt caaaaggaac attaaaaaca gtttgaatta ccggtcgttc
     2821 tgatgcaacc accacaattt cttcatcctc ataaaaaaat acaggtctaa ttcctgcagg
     2881 atcccgaaaa acaaaagcat caccatgtcc taaaaggccg gccatagcaa aaccaccatc
     2941 ccaattttta gaggcttttc tcaatatttt agcaatgtct aatttttctg caatcacagg
     3001 ggaagcttct cttttagaca agccattatt tttacattct tggtataaat cggtcacttc
     3061 atcatctaaa aagtgcccaa ttttttccat aacggttact gtatctgcta attctttcgg
     3121 atgctgaccc aattcaacca aattatcaaa aagttctttt acattagtca tattgaaatt
     3181 accggctaca atcaaatttc ggtgcatcca attgttttga cgtaaaaacg gatgtacact
     3241 ctcaatactg tttttgccga aagtaccata ccgaacgtgt cctaagaata attctccgat
     3301 ataaggaatg tgctcttttt gaagagctac attatcttgg tattccggat gagtagaaag
     3361 ttcttcgttt attcgctcat taatttgagc aaaaatatcc tggatgggtt gagcttgatt
     3421 agaacgaaca cgacttatat agcgttgccc gggttcaaca tcaaatttta tactggcaaa
     3481 accggctccg tcttgtccgc gattatgctg cttttccatc aaaagataca ttttttgtat
     3541 cccataaaaa gcagttccat atttgtcttt gtaatattga agcggttttt ttaatcgtaa
     3601 aagggcaatg ccgcattcgt gttttaagtt gtcactcatg atattctttt ggttgttgtg
     3661 ttgttttgac cgataatttc gtgttgtatt aattcttata agaataacaa atttgcaaat
     3721 ttgcggtgtt gtttaatcaa aaatgccccg aaaattcgag gcacaggttt ttattctatt
     3781 tctatatcaa attgtgtcaa ggcacgaaat tgttttaatc tgtcgtatac ttcttccttt
     3841 tctaagtcaa ccattcgttc agtgccaaac ttttcgacac aaaaagaggc tagatttgaa
     3901 ccatagatga taccgttttt catgttttca aaggatatat tttcactttg tgcaatatat
     3961 cctgaaaaac cgcctgcaaa agtatcaccg gctccggtag ggtcaaaaac ttcttctaat
     4021 ggtaaagcag gagcaaagaa aatttctttt ccttcaaata ataatgctcc gtgttctcct
     4081 tttttaatca caacatattt tggccccata gtgtggattt tagctgctgc ttttaccaat
     4141 gaatattcgc cggacaattg acgggcttct tcatcattta tggtaatcac atctacgcgt
     4201 ttcatcactt ggtgaaattc aggtaaagca caatccatcc aaaaattcat cgtgtctaaa
     4261 accactaatt taggtttctc agtcatttgg ttcagcacac tgatttgaac catcgggtgt
     4321 aaattgccta acatcaccac cgctgcatcc ttatattctg taggaacttt aggttggaaa
     4381 tccgctaata cgtttaattg agtttccaag gtgtctcttg aattcaaatc gttatgataa
     4441 cgaccgctcc agaaaaaggt tttcccacct tttacaattt caattcctga aatatcgata
     4501 tctctatcag ataataaatc taaatattct tgtggaaaat cttctccaac taccgaaaca
     4561 atagctgcat ccacatcaaa ataagaagca gaaagcccaa taaaagt
//
LOCUS       DAOTCW010000165         4591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_14437_length_4591_cov_24.274030, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000165 DAOTCW010000000
VERSION     DAOTCW010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14437_length_4591_cov_24.274030"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..717)
                     /locus_tag="P5335_10535"
     CDS             complement(115..717)
                     /locus_tag="P5335_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HRZ75358.1"
                     /translation="MSEVLLKYLPEHAVEPVFELIKTNSVHLKIVNERQTRHGDYRKH
                     PSGKHQITVNANLNKYRFLITLVHEIAHLAAFEKHGRYIKPHGVEWKMTFQRLMVPFI
                     RPEIFPNHVLPFVANHFRNPSASSDTDARLAFVLKQFDERKPDIHFIHEVPSGSFFRI
                     KNGKIFQKKGLRVKRYECLEVKSGKLYLFNANAEVEIIPG"
     gene            complement(732..1409)
                     /locus_tag="P5335_10540"
     CDS             complement(732..1409)
                     /locus_tag="P5335_10540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRZ75359.1"
                     /translation="MKNIIITGTSHGIGFELALQFANVGHQVLAISRKTPKELIEHSN
                     ITCLSIDLSSEKELYKVSDFLSQTWKNVDVIIHNAGSLLLKPFAQTSQEDFEKVYKVN
                     VFAVANLTRVCLPFLQKRSHVVTISSMGGIQGSMKFAGLAAYSSSKGAVITLSELLAE
                     EYREQGISFNVLALGAVQTEMLQEAFPGYEAPLSAKEMADYIVDFSLNGAKFFNGRVI
                     QVSSSTP"
     gene            complement(1456..2091)
                     /locus_tag="P5335_10545"
     CDS             complement(1456..2091)
                     /locus_tag="P5335_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75360.1"
                     /translation="MQKLKNIGVGFLVSFIGSIPLGYLNVVGFDIYSQKGLLVTIYYL
                     LGVVLIEFIVIFFTLIFAKKLSENKKLTKYIEGFSVLFMLVLAAFFYLSANSEITNRT
                     VFSNVSQNFFLAGLLFSSLNFIQIPFWTGWNLYLLNGNFIEISKSRKYFYICGTAAGT
                     FVGMLMLILSLHYFASNVDFLSQYLTTFIIPLVFFGLGIFQAFKFYKKYLR"
     gene            complement(2091..3125)
                     /locus_tag="P5335_10550"
     CDS             complement(2091..3125)
                     /locus_tag="P5335_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRZ75361.1"
                     /translation="MKLFIHKLFHWEYWPFQIVYFPIYFLWIFYAMKARSLFFFNACN
                     PTIRNGGFFAESKKEIYDLFPPQFYPKTELIPEKSTKEEMFSIFEKSSIAFPFIAKPD
                     IGLRGSAVKKIHNLSELEHYHNKASFDYLIQDLIPFANEVGIFYVRFPNQPKGKITGI
                     VGKEFLEVTGDGISTITALLKSNPRYELQLKSLQKEYGNQLNQVLEKGQKRNLVPYGN
                     HARGAKFVDVSHLISEKLTDVIDQMCQQIPDFYFGRLDVMYETWEDLENGKNFSIVEL
                     NGAGSEPTHIYDPKHSLFFAWYELARHITYMYQISVQNHQMGFRYLTFKEGLEQYRLQ
                     MQQQNKIYHF"
     gene            complement(3128..3838)
                     /locus_tag="P5335_10555"
     CDS             complement(3128..3838)
                     /locus_tag="P5335_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HRZ75362.1"
                     /translation="MCTVTFVSTNSKVIITSNRDEKVLRQKAIAPKDYLINGKKITFP
                     KDPKAGGTWFAVAEEGTISVLLNGANEKHELREKYRKSRGLIVLDIISAENPLLEWEM
                     IDLEGIEPFTIVLFHQNNLYQAQWNEINKTLVQLNVNENYIWSSSTLYSQVVRSQRAK
                     WFTEFIENNSSLNEESMMHFHRYTMEDNAEFGLVINRNDLLKTLSITQTVIENNRLTL
                     THVDLQNEEFFPNNQTSL"
     gene            complement(3826..4314)
                     /locus_tag="P5335_10560"
     CDS             complement(3826..4314)
                     /locus_tag="P5335_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRZ75363.1"
                     /translation="MLLFPIRKAMNELNDLLNQLTKDDYVYPCPFLSNASIGQHTRHI
                     IEMFQCLNVQYSEGVVNYDSRKRDRSIETDINTAKVAIENCLNELEKPNKSIRLVQMI
                     EGYEITTESNYNRELLYNLEHCIHHQALIKVAVLQLESVQISESFGVAPSTLEYRKQC
                     AQ"
     gene            complement(4398..>4591)
                     /locus_tag="P5335_10565"
     CDS             complement(4398..>4591)
                     /locus_tag="P5335_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRZ75364.1"
                     /translation="AMAKLYASEIALEVANEAVQIHGGNGYVAEYHVERMMRDSKITQ
                     IYEGTSEIQRIVISRGLVS"
BASE COUNT         1538 a          833 c          674 g         1546 t
ORIGIN      
        1 acgtgaacct actccaccgg ccattactat tgcgtaataa tttttgttca tttttttaag
       61 gtctaattat tctaatgatt gacaaaaata tagaaaataa gtccttaatg ttttctatcc
      121 cggaattatt tccacctcag catttgcatt aaacaaatac aactttccgg attttacttc
      181 taagcattca taacgtttaa cccgtaatcc ttttttttga aaaattttcc cgttttttat
      241 tctaaaaaaa ctaccggaag gaacttcatg aataaaatga atatcgggtt ttcgctcgtc
      301 aaattgtttt aaaacaaagg ccaatcgtgc atcggtatca ctacttgcag atggatttct
      361 gaaatgattg gctacaaaag gcaaaacgtg attgggaaaa atctccggac gaataaacgg
      421 aaccatcaac cgttgaaaag tcattttcca ttctactcca tgaggtttaa tgtatcgtcc
      481 gtgtttttca aaagcagcta aatgagcaat ttcatgaacc agcgtaatca aaaaacggta
      541 tttattcaaa ttggcattca ccgtaatttg atgctttcct gagggatgct tgcgataatc
      601 accatgacgc gtttgacgtt cgttcacaat ttttaagtga accgaattgg ttttaatcaa
      661 ctcaaaaaca ggttcaaccg catgttccgg gagatatttt aaaagaactt cactcattaa
      721 aaaactaaaa attatggtgt tgatgaactt acttgaatca ctcttccgtt aaaaaatttc
      781 gctccgttta acgaaaaatc aacaatataa tcggccattt ctttcgcaga aagtggtgct
      841 tcgtaacctg ggaaagcttc ttgcaacatt tccgtctgaa cagctccaag tgctaaaaca
      901 ttaaaagaaa taccttgttc tcgatattct tctgctaata attctgatag tgtgatcacc
      961 gctcctttac ttgaagaata ggctgccaat ccggcaaact tcatactccc ttgaatgcct
     1021 cccatcgaac tgatggttac aacatgactc cttttttgta aaaaaggcaa acaaacgcgt
     1081 gtcaaatttg ctacagcaaa cacatttact ttgtacactt tttcaaaatc ttcttgtgaa
     1141 gtttgtgcga atggttttaa caacaaactt cctgcgttat gtataattac atccacattt
     1201 ttccaagttt gagataaaaa atcggatact ttatataatt ccttttcact tgataaatca
     1261 atggaaagac aagtgatgtt agaatgttcg attaattctt tgggtgtttt tcgggaaatg
     1321 gctaaaactt gatgaccgac attggcaaat tgcaaagcta attcgaagcc aattccgtgg
     1381 gaagttccgg tgataatgat atttttcaat ttttttctaa ttttcgtaaa gataagaaaa
     1441 gaatggattg gaagattaac gcaaatactt cttgtaaaat ttaaaagctt gaaaaattcc
     1501 aagtccaaaa aatactaatg ggataataaa cgtcgtcaaa tattgagaca gaaaatcaac
     1561 attagatgca aaataatgaa gtgacaaaat cagcatcaac atgccaacaa atgttcctgc
     1621 agcagttccg cagatataaa agtattttct tgattttgaa atttctataa aattaccatt
     1681 taacagatat aaattccaac ccgtccaaaa gggaatttga ataaaattca aactgctaaa
     1741 aagtaatcct gctaagaaaa aattttgtga tacattagaa aaaacagttc tattagtaat
     1801 ttctgaattg gcacttaaat aaaaaaaagc cgctaaaact aacataaaca agaccgaaaa
     1861 accttcgata tattttgtta gttttttatt ctcggataat ttttttgcaa aaatcaaagt
     1921 gaaaaaaatc acaataaatt caatcaatac cacacctaat aaataataga tggttaccag
     1981 taatcctttt tgagaataaa tatcaaatcc aaccacattc aaataaccca aaggtatcga
     2041 accaatgaaa cttactaaaa aaccaacgcc tatattcttc aatttttgca ttaaaaatgg
     2101 tagattttat tttgctgctg catttgtaag cggtactgct ccagtccttc tttaaaggtt
     2161 aaataccgaa aacccatttg atgattttga acgctaattt gatacatata ggtgatatgt
     2221 cgagccaatt cataccacgc aaaaaacaac gaatgttttg ggtcataaat atgagtaggt
     2281 tcacttccgg caccatttaa ctcaacgata gaaaagtttt ttccgttttc taaatcctcc
     2341 caggtttcat acatcacatc taacctgcca aaataaaaat cagggatttg ctgacacatt
     2401 tggtcaatta catctgttag tttttctgaa attaaatgac ttacatctac aaatttagct
     2461 cctcttgcat gatttccgta gggaactaaa ttacgctttt gccctttttc taaaacttga
     2521 ttcaattgat tgccgtactc tttctgaagt gatttcaatt gtagctcata tcttggatta
     2581 gactttaaaa gtgctgtaat tgttgaaatg ccatcaccgg tgacttccaa aaattccttc
     2641 ccaacaattc ctgttatttt cccttttggt tgattgggaa agcgaacata aaaaattcca
     2701 acttcattgg caaacggtat taaatcttga atcagataat caaaactcgc tttgttatga
     2761 tagtgttcca attcagataa attatgaatt tttttaactg cagaaccacg taaacctata
     2821 tcaggtttgg caataaaagg aaaagcgata gaagattttt caaaaattga aaacatttct
     2881 tctttagtac ttttttcagg aattaactcc gttttcggat aaaattgtgg tggaaataaa
     2941 tcatatattt cctttttact ttcagcaaaa aatccgccat ttctaatagt tggattacac
     3001 gcattaaaaa agaagagact tcgtgccttc attgcataaa aaatccacaa aaaataaata
     3061 ggaaaataga caatttgaaa cggccaatat tcccaatgaa ataatttatg aataaacaat
     3121 ttcaaaatta aagggacgtt tgattgtttg gaaaaaattc ttcattttgc aaatcaacat
     3181 gcgtaagtgt aagtctatta ttttcgatta ccgtttgggt aatactgagt gtttttaaaa
     3241 gatcatttct attaatcact agaccaaatt ctgcattatc ttccatcgta tagcgatgaa
     3301 aatgcatcat cgattcttca ttaagtgagg aattattttc aataaactcg gtaaaccatt
     3361 ttgctcgttg gcttctaaca acttgagaat acaaagttga cgaagaccaa atataatttt
     3421 cattaacgtt taattgtacc aatgttttat ttatttcatt ccattgtgct tgatataaat
     3481 tattttgatg aaacagaaca attgtaaatg gttcaatacc ctctaaatca atcatctccc
     3541 attccaaaag aggattttct gcacttatga tgtccagaac aataagtcct ctgctttttc
     3601 tgtatttttc tctaagctcg tgtttttcat ttgccccatt taacagaact gaaatggttc
     3661 cttcttcagc aactgcaaac caagttccac ctgcttttgg gtctttggga aatgttatct
     3721 ttttaccatt tatcaaataa tctttaggtg caatagcctt ttgacgcaac accttttcat
     3781 ctctatttga ggtgatgatt actttactat ttgtggaaac aaatgttact gtgcacattg
     3841 ttttctgtat tcaagggttg aaggtgccac accaaaactt tcgctaattt gtactgactc
     3901 caattgcaaa acagctactt taattaatgc ttgatggtga atacaatgtt ccaaattata
     3961 cagcagttct cggttgtaat tactttcggt tgtaatttca tacccttcta tcatttgaac
     4021 caaccgaata gatttatttg gtttttccaa ttcattcaaa caattttcaa tggctacttt
     4081 tgccgtattt atatctgttt caatggaccg atctcttttt cgggaatcgt aattaacaac
     4141 accctcagaa tactgaacat ttaagcattg aaacatttca ataatgtgtc ttgtatgttg
     4201 gccaattgac gcattactca agaacggaca aggataaaca taatcatcct ttgtaagttg
     4261 atttagtaaa tcgttcaatt cattcattgc ttttcgaata ggaaataaca gcatacactt
     4321 tcaatttttc tcaaaaataa caaaaaaacc ctttgagaaa tttctcaaag ggtttaattt
     4381 ataaaattta aatttgctta tgaaaccaat cctcttgaaa ttacaatgcg ttgaatttca
     4441 gatgttcctt cgtaaatttg tgtaattttt gaatcacgca tcatacgttc tacatggtat
     4501 tctgccacat atccgtttcc gccgtgaatt tgaacggctt cattggccac ttctaaagca
     4561 atttcagaag catataattt tgccattgca c
//
LOCUS       DAOTCW010000166         4553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_14592_length_4553_cov_38.742330, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000166 DAOTCW010000000
VERSION     DAOTCW010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14592_length_4553_cov_38.742330"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="P5335_10570"
     CDS             <1..990
                     /locus_tag="P5335_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRZ75365.1"
                     /translation="NSLKFSARYLLSLVNDLLQINKIEENKVVLENLAMNIADEIKMV
                     SNSLSFIASRNNNKIVTQIDSKIPEYLVGDKLRLSQILMNLVSNGLKFTQDGEVRIIV
                     NLERNEKSNCFINFVVEDTGMGIAEKDLGKIFDKFVQIDRKEDDYQGAGLGLSIVKKL
                     ITLFGSEISVESKEGQGTKFTFTIGFDSDITKVNEIINNIQVDLSTDQVYKVLVVEDN
                     KINQMVTRKIMEKNNFKTIIVDDGYAAIEALNKESFDVVLMDINMPLINGFDTTRLIR
                     KKGITIPIIALTAFAKDEISEEAISSGMNDVLVKPFEQANLIHCIETLVKKTS"
     gene            complement(987..2240)
                     /locus_tag="P5335_10575"
     CDS             complement(987..2240)
                     /locus_tag="P5335_10575"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRZ75366.1"
                     /translation="MTFQDLNLLQNIQHALSDEGYTTPTPIQQQAIPIILEGTDLVGC
                     AQTGTGKTAAFAIPILNTLHRIVGSAQKRKNIRTLVVTPTRELAIQIGESFDTYGKYT
                     NVRQITIFGGVSQVPQVDQLKKGVDILIATPGRLLDLHKQGFIDLDHLHYLVLDEADL
                     MLDMGFINDVKKIIKLTPSNRQTLLFSATMPIAIREIADSYLTKPKYVSVTPISSTAE
                     TVEQKIYFVGKEDKRKLLYHLIRNENLNNLLVFTRTKHGADNVVKALKKNGVNAEAIH
                     GDKSQTARQRVLESFKNKEISVLVATDIAARGIDIESLPYVLNFDLPNIPETYVHRIG
                     RTGRAGNGGISISFCGKDEEPYWKDIQKLVKVNVKVIKDHPYPWNTAEESAVIEPEKK
                     DYRNVTKQSPSRKSDASKKNKKRWY"
     gene            complement(2316..3356)
                     /locus_tag="P5335_10580"
     CDS             complement(2316..3356)
                     /locus_tag="P5335_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HRZ75367.1"
                     /translation="MENLVPFTSIDLGKITSHRSGEIKFGEKMLTIPKDVDTIQFLNE
                     CKAKFVLFGIPEDIGVRANAGRPGAASAWESAIKSIANIQHNRFCKGSQLLILGSLDV
                     REELKQAENLNFHDVDDRKKFSILVEKLDKEVSHIIFNIVKAGKIPIIIGGGHNNAYG
                     NIKGTALAKGKPINAINLDAHSDFRILEGRHSGNGFSYSFDEGFLKKYFIFGLHESYT
                     SKNVLLRLKDLEDRVRFNTYDEISIRKVKNFEQELNQALSFIKNDCFGIEIDLDSIPN
                     IASSAMTPSGFSVEQARQLIHHFGQHANASYLHICEGAPDLCDEKNPQMIGKLMGYLI
                     TDFIKAKNILTE"
     gene            3460..4344
                     /locus_tag="P5335_10585"
     CDS             3460..4344
                     /locus_tag="P5335_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75368.1"
                     /translation="MENQNNNSKLKAVILVLSLLLLASLGYMFKMSSDAKKVETELSS
                     VTTEKENALTELAQLKTTYDAAIAENTSMSEELIAERDKVVKLMEEVKKSKGDAAAIG
                     KYKQQIADLQAKMNVLIRENDELKVKNQQLTAEIDSTKTILVEAKEYNQVLAGQNEEL
                     SKTVEKASKLSILNLKTGAYKVKSSGKEIETERARRADILKVNFTIAENKVAKSGDKS
                     YYIQVIDSKNNVLGEKKLVEFGEKSLTYSFISTVQYENSTVNVSENLPGENFEKGTYF
                     VNIFDKAELVGKTSFTLK"
     gene            complement(4386..>4553)
                     /locus_tag="P5335_10590"
     CDS             complement(4386..>4553)
                     /locus_tag="P5335_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRZ75369.1"
                     /translation="NGAYAMGVSNTHGSITKGKKANIILTKPIHSFYQFPYEFGSNLI
                     EKVFIEGKLIS"
BASE COUNT         1581 a          718 c          728 g         1526 t
ORIGIN      
        1 aattctttga aattctcggc acgttatctg ttatcgttag taaatgattt acttcaaatt
       61 aataaaattg aagaaaataa agtcgtttta gaaaatttag ccatgaatat tgcagatgaa
      121 ataaaaatgg tttctaattc actttccttt attgcttctc gaaataataa taagattgtc
      181 actcaaattg attcgaaaat tccggaatat ttagttggtg acaaactccg actttcacaa
      241 attctaatga atttagtcag taatggatta aaattcacac aagatggtga agttcgtatt
      301 attgttaatt tagaaagaaa tgaaaaatca aattgtttta tcaattttgt tgtagaagat
      361 acagggatgg gtatcgctga gaaagattta ggtaaaatat ttgacaagtt tgttcaaatt
      421 gacagaaaag aagatgatta tcaaggtgca ggattaggtc tttccatagt aaaaaaatta
      481 ataaccttat ttggcagtga aatttcagtc gaaagtaaag aaggtcaagg aaccaaattc
      541 acatttacca ttggatttga ttcggatatt actaaagtaa atgaaatcat caacaatatt
      601 caagttgatt tgtcaaccga tcaggtctac aaagttttgg tggtggaaga caacaaaatt
      661 aatcaaatgg tcacacgaaa aatcatggag aaaaacaatt tcaaaacaat aatcgttgat
      721 gatggttatg ctgcgataga agcattaaat aaggagtcat tcgatgttgt tttgatggac
      781 atcaatatgc cactgattaa tgggtttgat actaccagat taatcagaaa aaaaggaata
      841 acaattccta ttattgcttt gacggctttt gctaaagatg aaatttcaga agaagcaatt
      901 tcttccggaa tgaatgatgt attagttaaa ccttttgaac aagcaaatct gattcactgt
      961 attgaaactt tggtcaaaaa aacatcttaa taccaacgtt ttttattctt ttttgaagca
     1021 tcggattttc gtgaaggcga ttgttttgtt acatttctgt aatctttttt ttcaggctca
     1081 ataaccgcac tttcctcagc agtattccaa ggatatggat gatctttaat tactttgaca
     1141 tttactttaa ccaatttttg aatgtctttc caatacggtt cttcatcttt tccacaaaag
     1201 gaaatagaaa tccctccatt tccggcacga ccggttctac caatgcggtg aacataagtt
     1261 tcaggaatat tcggcaaatc aaaatttaaa acatacggta aactctctat gtcaattcct
     1321 ctagcagcaa tatcagtggc tactaatact gaaatttctt tgtttttaaa actttccaaa
     1381 acacgttgcc ttgctgtttg cgatttatcg ccatgaatag cctctgcatt taccccattt
     1441 ttcttaagag cttttaccac attgtccgca ccatgctttg ttctggtaaa tactaaaaga
     1501 ttgtttaaat tttcatttcg gattaaatga taaagcaatt ttcttttgtc ttctttacct
     1561 acaaaataga ttttttgttc taccgtttct gccgttgaag agataggggt taccgaaaca
     1621 tattttggtt ttgtcaaata actatccgca atctctcgaa tggctatagg cattgttgct
     1681 gaaaacaaaa gtgtttgtct attggaaggt gttaatttga tgattttttt tacatcatta
     1741 ataaagccca tatctaacat taaatcagct tcatctaaaa ctaaataatg caaatggtct
     1801 aaatcaataa aaccttgttt gtgaaggtct aataatcgac ccggggtagc aattaaaata
     1861 tctactcctt tttttaattg atctacttga ggaacttggc tgactccccc aaaaatggtg
     1921 atctgtctaa cattagtata tttaccatag gtatcaaaac tttctcctat ttgaatggct
     1981 aattcacggg taggggtgac taccaatgtt ctgatattct ttcttttttg agcagaacct
     2041 actattctgt gcagtgtatt taaaattggg atggcaaaag cagccgtttt tccggtccct
     2101 gtttgagcac aacctactaa atcagttcct tctaatataa ttggaattgc ttgttgctga
     2161 ataggagtag gggtagtata accttcatca gaaagagcat gttgtatatt ttgtaataga
     2221 tttaagtctt gaaatgtcat aaaaattatt tgccggcaaa gataggttaa ataaattaga
     2281 cctaaaaaga cttagctttt tgacatttta agtagttatt ccgttaaaat attttttgct
     2341 ttgataaaat cagtgattaa ataccccatt aatttaccta tcatttgtgg atttttctca
     2401 tcacataaat ccggagctcc ttcacaaata tgaagataag aagcgttagc atgttgtcca
     2461 aaatgatgaa ttaattgtct agcctgttct actgaaaaac cacttggagt catagcacta
     2521 ctggcaatgt ttggaataga atctaagtca atttcaattc caaaacaatc atttttaata
     2581 aaggacaagg cttggtttaa ctcttgttca aagtttttta ctttacgaat agaaatctca
     2641 tcatacgtat tgaatcgaac gcggtcttct aaatccttta atcttaataa aacatttttt
     2701 gaagtgtaac tttcatgcaa accaaaaata aagtactttt ttaaaaaccc ttcatcaaat
     2761 gaatatgaaa aaccattgcc gctgtgcctt ccctctaaaa ttctaaaatc agaatgagca
     2821 tctaaattaa tcgcattaat aggttttcct ttagctaaag cagttccttt aatatttccg
     2881 taggcattgt tatgaccgcc accaataata attggaattt ttccggcttt tacaatgtta
     2941 aatataatat gagacacttc tttatctaat ttttctacta aaatggagaa ttttttgcga
     3001 tcatcaacat catgaaaatt aagattttca gcttgtttaa gttcttctct gacatcaagt
     3061 gagcctaaaa taagcagttg actcccttta caaaatcgat tatgttgaat gttcgcgatg
     3121 cttttaatgg cactttccca cgctgaagct gcaccgggtc ttccggcatt tgctctcaca
     3181 ccaatgtctt ccggaattcc gaataaaaca aattttgctt tacattcgtt tagaaattga
     3241 atggtatcga cgtcctttgg aattgtaagc attttttcac caaattttat ttcaccacta
     3301 cggtggctgg taatcttgcc taaatctatc gaagtaaacg gaactaaatt ttccataaaa
     3361 aatatattgc tccaaaaata ctatttttat ataaaataac gttatggtat tgcaattcga
     3421 atctaaataa aatttaactt tgtctaaaat caacacagta tggaaaacca aaacaacaat
     3481 tcaaaattaa aagcggtaat attagtattg tcgctattgt tattagccag tttaggctat
     3541 atgtttaaaa tgagctctga cgctaaaaaa gtagaaacag agttgtcctc agttactact
     3601 gagaaagaaa atgctcttac ggaattagct caattgaaaa cgacctatga tgcagctatt
     3661 gcagaaaaca cttcaatgtc tgaagaatta attgctgagc gtgacaaagt agttaaatta
     3721 atggaagaag ttaaaaaatc aaaaggtgat gccgctgcaa tcggtaaata caaacaacaa
     3781 attgctgatt tgcaagctaa aatgaacgtt ttaattcgtg aaaatgatga gttaaaagtt
     3841 aagaaccaac agttaacagc tgaaattgat agtacaaaaa ctattttagt tgaagctaaa
     3901 gaatacaacc aagtattagc aggacaaaat gaagaattgt ctaaaacagt agaaaaagct
     3961 tctaaacttt ccattctaaa tttaaaaacc ggagcctata aagtaaaaag ctcaggtaaa
     4021 gaaatagaaa cagagcgtgc aagaagagct gatattttaa aagttaactt tacaattgct
     4081 gaaaacaaag tggccaaatc tggagataaa tcttattata tccaagtaat tgacagcaaa
     4141 aacaatgttt taggtgaaaa gaaattagtt gagtttggtg aaaaatcatt aacctacagt
     4201 tttatttcaa cagttcaata tgaaaatagt acagtaaatg tttctgaaaa tcttccaggt
     4261 gaaaattttg aaaaaggaac ttattttgtg aatatttttg acaaagctga attagttgga
     4321 aaaactagtt tcactttgaa ataagaaata aataacagtt aaaaaggggc ttagtgcccc
     4381 tttttttatg aaattaactt tccttctata aatacttttt caataagatt ggagccaaat
     4441 tcatacggaa attgataaaa cgaatgaatt ggttttgtta aaattatatt ggctttttta
     4501 ccttttgtaa tactcccgtg tgtatttgaa actcccatag cataagctcc att
//
LOCUS       DAOTCW010000167         4540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_14653_length_4540_cov_34.100780, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000167 DAOTCW010000000
VERSION     DAOTCW010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14653_length_4540_cov_34.100780"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1480
                     /locus_tag="P5335_10595"
     CDS             101..1480
                     /locus_tag="P5335_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRZ75370.1"
                     /translation="MKKLLQFCVLLFSMSSFSQTIGLQTFATGFSSPVAIVNAGDSRL
                     FVVQRGGLIRILNSNGTINTTPFLSLTSIITAGGERGLLGLAFHPDYATNGRFYVNYT
                     RSGDGATVIAKYTVSDSDPNVANSASAEILLTIAQPFQNHNGGSLVFGPDGYLYIGMG
                     DGGDGGDPNNYGQNINSLLGKMLRIDVDSGSSYSIPSTNPYAGATPGANEIWATGMRN
                     PWKFSFDRVTGDLWIADVGQNAIEEINKAASTEAGLNYGWRCYEGNAAYNIDGCGAAS
                     NYTFPVAEYTHASTGGCSLTGGYVYTGTTYPNLLNKYLFADYCTNKIGYISTTGGAIT
                     WSSNTFTGNFVSFGEDINGELYIAGISNGTISRIIDTSLSTNDFQRNGLSLYPNPAHN
                     SFTIKNSNFLNLTTLTIYDSMGKRVANQKMDDLELTIVSIDHLTSGLYFVSVEDMNGG
                     SFTTKLVVK"
     gene            complement(1565..2599)
                     /locus_tag="P5335_10600"
     CDS             complement(1565..2599)
                     /locus_tag="P5335_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HRZ75371.1"
                     /translation="MKHTTLPNTSLKISKICLGTMTFGEQNTEAEAHQQLDYAVSSGI
                     NFIDTAEMYPIAAREATLGSTERYIGSWLKTRGKRDDLVIATKIAGPNRGMDYIRKPL
                     DFSKKSILEAVDLSLKNLQTDYIDLYQMHWPERVMNMFGQRGLVKIDVNWEENFFEVL
                     SVYNELIKAGKIRHIGVSNEAPWAVMKFISESEKHHLPRIATIQNPYSLLNRLFEVGL
                     AEVCLRERVGLLAYSPLGFGILSGKHLTEIQPNSRIGLFPQFTRYTSENSTRATRLYQ
                     ELAFDNDLTLAEMALAFVQHQSFVTSTIVGATSLEQLKENTNSHSIVLSEAILKEINV
                     IQEMIPNPAP"
     gene            complement(2706..3671)
                     /locus_tag="P5335_10605"
     CDS             complement(2706..3671)
                     /locus_tag="P5335_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRZ75372.1"
                     /translation="MIKKVSLLVLVAALATSCVSKKVYNELESKFAALKKEQRQLSDE
                     NHDLEKTKNKLEVDLKANQDELARIKAERDKLQNELATASTNLKTLTASYTALEKNSD
                     EALQANMQKNRELLAQLDAKEKALLAEQERLNKLQADLKERSQRVTDLENLISSKEES
                     MRKLKETLSKALNSFEGKGLTVEQKNGKVYVSMENKLLFQTGSWAVGTEGKRAVVEVG
                     KVLAQNPDISVLIEGHTDNDKILGSIGGGIENNWDLSTKRATAIVNILAENKGINKQN
                     LTAAGRGEFAPLMSNDSPEGKAKNRRIEIILTPKLDEISKMLNDL"
     gene            complement(3713..4474)
                     /locus_tag="P5335_10610"
     CDS             complement(3713..4474)
                     /locus_tag="P5335_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRZ75373.1"
                     /translation="MRIISYNVNGLRAAITKGFLDWLQQANPDVICLQEIKATEEQIP
                     TAEIAKAGYPYQYYFPAQKKGYSGVAILSKIQPKNVVFGTGIASMDFEGRNVRADFDD
                     FSVMSLYLPSGTNIERLDHKFNYMDEFQQYINALKVEVPNLIIGGDYNICHEAIDIHD
                     PIRNATVSGFLPEERRWLDGFMKSGFIDSFRHFNKEPHQYSWWSYRANARNNNKGWRI
                     DYHLVSEPMRNRLKRAVILPEAKHSDHCPILVEIE"
BASE COUNT         1367 a          850 c          768 g         1555 t
ORIGIN      
        1 gccttgaaga aatttggggc ggtttttttg taacctgtta ataatgaaaa ctattaaaaa
       61 aaaattacta aatttacctc catccaaaaa ctaaaaggct atgaaaaaac tattacaatt
      121 ttgtgtttta ctattctcca tgtcatcctt ttcgcaaacc atcgggttgc aaacttttgc
      181 taccggtttt tcgagtccgg tggctattgt caatgctggt gattctagac tatttgttgt
      241 acagcgaggt ggattaatca gaatattgaa ttccaacgga accattaaca ctacgccttt
      301 cctatcgtta acatcaatca ttactgcagg cggagaacgt ggcttattag gattggcttt
      361 tcatccagat tatgccacaa atggtcgctt ttatgtaaac tatactcgtt caggtgatgg
      421 tgccacagtg attgcaaaat ataccgtgag cgattctgat ccaaacgttg caaattctgc
      481 cagtgcagaa atactgttaa cgattgctca accttttcaa aatcacaatg gcggttcatt
      541 agtttttggg cctgatggtt atctatatat aggaatgggt gatggtggtg atggtggtga
      601 ccctaataat tacggacaaa acatcaactc attattaggc aaaatgcttc gaattgatgt
      661 tgatagcggc tcatcttatt cgattccatc aaccaatcct tatgccggag caactccggg
      721 agcaaatgaa atttgggcaa ccggaatgcg aaatccatgg aaattttcat tcgacagagt
      781 aacaggtgat ttatggatag ccgatgtagg tcaaaatgcc atcgaagaaa tcaataaagc
      841 agcttcaaca gaggcaggtt taaattatgg atggcgttgt tatgaaggaa atgctgctta
      901 caatattgat gggtgcggag cggcttccaa ttatacattc ccagtagcag aatatactca
      961 tgcttcaaca gggggctgtt cattaaccgg tggatatgtt tacaccggca ccacttatcc
     1021 aaacttacta aacaaatatc tttttgctga ttattgtaca aataagatag gatatatttc
     1081 cactacgggt ggagcaatca cttggtcttc taatactttt accggaaatt ttgtttcttt
     1141 tggtgaagat attaatggcg aactttatat tgccggaatc tctaatggaa ccatttccag
     1201 aatcatagat accagtttga gtacgaacga ttttcagcga aacggattga gtttatatcc
     1261 taatcccgcc cataattcat ttacaattaa aaattccaat tttttgaatt taaccacatt
     1321 gacaatttat gattcgatgg gaaaaagagt tgctaatcaa aaaatggatg atttagaatt
     1381 aacaattgtt tcgattgatc accttacttc cggattgtat tttgtgtcgg tggaggatat
     1441 gaatggaggt agttttacaa ctaagttagt ggtgaaataa taattaaacc ttaaaagaat
     1501 gagtttcatt aaaaacgagc ttattctttt aagggtttga aatttatctc ttaatttaaa
     1561 aaaatcaagg tgccggattt ggaatcattt cctgtatcac gttaatttct ttcaaaatcg
     1621 cttcagaaag aacaatgcta tgggaattag tattttcttt taattgttcc aacgaagttg
     1681 ctccaacaat tgtgctggta acaaatgatt gatgttgcac aaaagccaat gccatttcgg
     1741 ctaacgtcaa gtcattgtca aaagctaatt cctgatacaa tcgggttgcc cttgttgaat
     1801 tttctgaagt atatcgtgta aattgaggaa acaatcctat tctggaatta ggttggattt
     1861 ctgtcaaatg ttttcctgat aaaataccaa atcctagcgg agaataggcc aataatccca
     1921 ctcgttctcg caaacaaact tctgctaaac ctacttcaaa caaacgattt aatagtgaat
     1981 acgggttttg aatcgttgca atacgaggta aatgatgctt ttcgctttca ctgataaatt
     2041 tcatcactgc ccaaggagct tcattggaaa ctccaatatg tctaatttta cctgctttga
     2101 ttaattcatt gtatacagat aaaacttcaa aaaagttttc ttcccaattt acatcaattt
     2161 taactaaacc acgttgtcca aacatattca tcactctttc gggccaatgc atttggtata
     2221 aatcaatgta atccgtttgc agatttttca aactcaaatc aacggcttct aaaatacttt
     2281 tctttgaaaa atctaatggt tttcgaatat aatccattcc gcgattgggt ccggctattt
     2341 tagtggcaat aactaaatca tctcttttgc ccctagtttt tagccaagaa cctatgtatc
     2401 gttcagtgct tcccaatgtt gcttcacgag ctgcaatagg atacatttcc gccgtatcta
     2461 taaaatttat tccactggaa acagcataat ctaattgttg atgagcttca gcctcggtat
     2521 tttgctcacc gaaagtcatc gttcctaaac atattttgct gatttttaag gaagtgtttg
     2581 gtaatgtagt gtgtttcatt ttgaggtact aaggtactaa gggactgagg gactgaggga
     2641 ctgagggact gagtaaagca aagttaaacc taagcaactt aatccctcag cacctttgaa
     2701 cttatttaca aatcatttaa cattttagaa atctcatcta atttcggcgt caaaataatt
     2761 tctattctgc ggttttttgc ttttccttcc ggagagtcat ttgacattaa tggagcaaat
     2821 tcccctcttc cggctgccgt taaattttgt ttattgattc ctttattttc agctaaaata
     2881 ttcacaatag cagtagctct ttttgttgag agatcccagt tattttctat tcctccacca
     2941 attgaaccta atattttatc attgtcggta tgcccttcaa ttaaaacaga aatatcaggg
     3001 ttttgagcta atactttacc aacttctaca actgctcttt ttccttcagt tccaacagcc
     3061 caacttccgg tttgaaataa taatttattt tccatggaaa cataaacttt tccgtttttt
     3121 tgttccacag taagcccctt cccttcaaaa ctattcaatg cttttgaaag tgtttctttt
     3181 aattttcgca tgctttcttc tttcgatgaa attaggtttt ctaaatcagt tacacgttgc
     3241 gaacgttcct ttaaatcagc ttgtaacttg tttaagcgtt cttgttctgc taacaatgct
     3301 ttttcttttg cgtctaattg tgccaataat tctctgtttt tctgcatgtt agcctgcaaa
     3361 gcttcatcag aatttttctc caaggcggta taagaagcgg tcaatgtttt taaattagta
     3421 gatgctgtag ctaattcatt ttgcaatttg tctctttccg cttttattcg agccaattca
     3481 tcctgattgg cttttaaatc aacttctaat ttatttttag tcttttctaa atcatggttt
     3541 tcatcagaaa gttgacgctg ctcttttttc aaagctgcaa atttgctctc taattcatta
     3601 tacacttttt tggacacgca agaagtagcc aaggcggcaa ctaaaactag taatgaaact
     3661 tttttaatca ttatttaaag tatttgaggt ttttattaat aaaattattg gcttattcta
     3721 tttccactaa aatcggacaa tggtcagaat gttttgcttc cggaagaata accgctcttt
     3781 ttaatcgatt acgcattggt tcgctcacta aatgataatc tattcgccaa cctttattat
     3841 tgtttctggc attggctcgg taactccacc aggaatattg atgcggttct ttattaaaat
     3901 gacgaaaact gtctataaaa ccacttttca taaaaccgtc taaccaacgt cgttcttcag
     3961 gcaaaaaacc ggaaactgtt gcattccgta tcgggtcgtg gatgtcaatt gcttcatgac
     4021 aaatgttata atcaccacca ataatcaaat taggaacttc aactttaagt gcgttaatat
     4081 actgttgaaa ttcatccata taattaaatt tatgatccaa ccgttcaata ttggtacctg
     4141 agggtaaata taaactcatt acagaaaaat catcaaaatc agctctaaca tttcgccctt
     4201 caaagtccat cgaggcaatt cctgttccaa aaactacatt ttttggttga attttagata
     4261 gaatagcaac cccactatat cctttttttt gagccgggaa ataatattga taaggataac
     4321 ccgctttggc aatttcagct gtaggaatct gttcctcagt agctttaatt tcctgtaaac
     4381 aaattacatc cggatttgcc tgctgaagcc aatctaaaaa acctttggta atggctgctc
     4441 gtagaccatt tacattataa gaaataatac gcatttggaa ttttcagtaa agtttttcaa
     4501 aaatagataa aaaccttgag tatcaaaaca gactggtgga
//
LOCUS       DAOTCW010000168         4521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_14752_length_4521_cov_47.320421, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000168 DAOTCW010000000
VERSION     DAOTCW010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14752_length_4521_cov_47.320421"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1968)
                     /locus_tag="P5335_10615"
     CDS             complement(<1..1968)
                     /locus_tag="P5335_10615"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRZ75374.1"
                     /translation="MKKLLFFGLFPALLLAQEKTETKRESGHYDTNKFSQVYDLLATP
                     NMFRTASGAPGPAYYQQQADYKMDVVLDDKNKRIDGVETITYHNNSPESLEYLWVQLD
                     QNMRARQSKTPLIESQKMEGSVPVDGFVKNYMQQTFDGGFNLVYVNDANGKPLSYVIN
                     QTMMRIDLPTPLKPKEKVSFSIKWWYNINNYRVDGGRSGYEEFEKDGNRLYVIAQFFP
                     RMAVYNDVEGWQNMQFWGRGEFALAFGNYDVNITVPADHVLEATGVLLNRKEVFTAEQ
                     VKRYELAEKTFDKPVLIVTPDEAKATEKGFSDKTKTWKFRAENVRDFAFSSSRKFIYD
                     AMAVKLETKNVMAISLYPNESNPLWEQYSTKAVAQTLKTYSKHTFDYPYHKAISVSAE
                     DQGMEYPMICWNYGRPDANGYVSDRIKYGMISVIIHEVGHNYFPMIVNSDERQWSWMD
                     EGLDTFLQYLAEQDFMENYPSSRGPAHKIVPYMSGDQKFLEPIMSNSEGIHQFGANAY
                     SKPATGLNILRETIMGRELFDHAFKTYANRWKFKHPTPEDFFRTMEDASAVDLDWFWR
                     GWFFSTDYVDIGIKEVKQYFVTDEPTAELKDIKARRGRFGTAAGPYVYLVEGNNPQLN
                     QKNKKDLTISEIKPLNDFVTTKFNAEEQRNLK"
     gene            complement(2026..2529)
                     /locus_tag="P5335_10620"
     CDS             complement(2026..2529)
                     /locus_tag="P5335_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75375.1"
                     /translation="MRKKIKIVFIAIFFIGLCSFTMHKFYVSVTQINFVPEKKVIQIT
                     ARFFIDDLNNALEKKHKTKFYLGSSKESAVQLEQFKKYISDNFSIKVNAKFKEIQFVQ
                     KEIEDDVFIIYLKVSDVSKVNSIEVKNTLLFDFITEQQNIIHTQVLNNKQSALLTPDN
                     PNELLNY"
     gene            complement(2535..3323)
                     /locus_tag="P5335_10625"
     CDS             complement(2535..3323)
                     /locus_tag="P5335_10625"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRZ75376.1"
                     /translation="MKNNFLLIATFFIVQFSYGQFVPRVVLKGKIVAESLSIDNVTVF
                     NVSSNKGAISDHLGYFTLYARPSDTLVFSSVVFKSKKLILAEIDFKVEVVYVKLEEFI
                     NELDEVVVTPKTLTGDLIKDVKNMKVTDMNPAFDSGQITDLQFEKDASSTPKNPGIYD
                     GTITYGMDFVKIGKMIGKIFVKDKPKEMVFTSSKNFQEAVKDKFTYNFFTETLELNKD
                     EIGLFLAFCDSDPKVKELLAVNREIDLIDFLISKSKEYKNLPKD"
     gene            complement(3313..4029)
                     /locus_tag="P5335_10630"
     CDS             complement(3313..4029)
                     /locus_tag="P5335_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75377.1"
                     /translation="MNKVLFLLFIFSSAKVFSQNEKQIRGIIKVENASVDGIHVLNLV
                     SEKATITNAKGEFYLEVNEDDLLVFSAVHLNYWRKTITKKEINDGFVEINMTSKVNQL
                     EEVVVSENTKITAQNLGIITYKPVSYTPAERRIRTATTGFLDPLLNWVSGRTKNLKRE
                     LEVEKRERLIIRLESYFEDAFFINSLKIPKEYVNGFKFYVVEDINLAEALTSKNKVRA
                     TFILGELSQEFLTYLNSYEK"
     gene            complement(4022..>4521)
                     /locus_tag="P5335_10635"
     CDS             complement(4022..>4521)
                     /locus_tag="P5335_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75378.1"
                     /translation="DFKTGFLEVIMTPKEVKLNEVVLTEYTKINAQELGIINYKPKAY
                     TPAERKLRPTKITQMDLIGLMAFRLPLDPLLYWATGRTKMLKNDLKIEKKELLLRKLN
                     DWNDNEFYANQLKIPKEFVDGFKYYIIYDDELMSYLQSNNKIQGNFRISKLATDFLIY
                     MEEYE"
BASE COUNT         1437 a          821 c          620 g         1643 t
ORIGIN      
        1 ttttaaatta cgttgttctt ccgcattgaa tttagtggta acaaaatcat tcaagggttt
       61 aatttctgaa atagttaaat cctttttatt tttttgattg agttgtggat tatttccttc
      121 cactaaatat acataaggtc cggctgctgt gccaaatcta cctcttcgtg cttttatgtc
      181 ttttaattcc gctgtaggtt catcggttac aaagtattgt ttgacttctt tgattccaat
      241 gtccacataa tcggtggaga aaaaccaacc tctccaaaac caatctaagt caaccgcgga
      301 agcatcttcc attgttctaa aaaaatcttc aggcgttgga tgtttaaatt tccatcggtt
      361 agcatacgtt ttaaaagcat ggtcaaacaa ttcgcgaccc ataatagttt cacgcaaaat
      421 attcaatccg gttgccggtt tagaataagc attagctcca aattggtgaa taccttcaga
      481 attagacatg attggttcta aaaacttttg gtcgccactc atataaggca ctattttgtg
      541 agccggacca cgacttgacg ggtaattctc catgaaatct tgttcagcta aatattgtaa
      601 aaacgtgtct aagccttcat ccatccatga ccactggcgt tcatcagaat tgacaatcat
      661 cggaaaataa ttatgtccga cttcgtgaat aattacgcta atcataccgt atttgattcg
      721 gtctgaaaca tatccgttgg catctggtcg accataattc caacaaatca ttgggtattc
      781 cattccttga tcttcagcag aaacagaaat tgctttatga taaggataat caaacgtatg
      841 tttagaatat gtttttaacg tttgtgccac cgctttggta gaatattgtt cccacaacgg
      901 attgctctca ttaggataaa gagaaatagc catcacattt ttggtttcta attttactgc
      961 catggcatca taaatgaatt ttctagagga tgaaaatgca aagtctctca cattttcagc
     1021 tctgaatttc catgtttttg ttttatcaga aaaccctttc tcagttgctt ttgcctcgtc
     1081 cggtgtaaca attaaaacgg gcttatcaaa tgttttttca gcaagttcat atctttttac
     1141 ttgttcagcc gtaaaaactt cttttctatt caataaaact ccggtagctt ctaacacatg
     1201 gtctgccgga acagttatat tcacgtcata attcccaaaa gccaaggcaa actctcctct
     1261 tccccaaaac tgcatatttt gccagccttc cacatcatta taaacagcca ttcttggaaa
     1321 aaattgagca ataacatata atctatttcc gtctttttca aactcttcat aaccggaacg
     1381 gccaccatct accctataat tattgatatt gtaccaccat ttaattgaaa aagaaacttt
     1441 ctcctttggt tttaatggag ttggtaaatc aattcgcatc atggtttgat taataacgta
     1501 ggaaagtggt tttccattag catcatttac ataaaccaaa ttaaatccac catcaaaagt
     1561 ttgttgcatg taatttttca caaatccatc aacaggaact gagccctcca ttttttgact
     1621 ttcaattaat ggcgttttcg attgtcgggc tctcatgttt tggtctaact gaacccaaag
     1681 atattctaat gattcaggtg aattattgtg ataagtaatt gtttcaactc catcaatacg
     1741 tttattttta tcatctaaaa caacatccat tttataatct gcctgttgct gataataagc
     1801 cggccctggt gcaccggagg ccgttctgaa catattgggt gtagccaata aatcatagac
     1861 ttggctaaat ttattcgtgt cgtaatgacc tgattctctt tttgtttcag ttttttcttg
     1921 tgccaacaat aaggctggaa ataatccgaa aaatagcagc ttcttcatct aaattttttt
     1981 tgaaggaact aaaataaaca ttttaaagaa gcagaaattt ttttatcagt aatttaacaa
     2041 ctcgttggga ttatcaggag ttaacaaagc actttgcttg ttatttaaaa cttgggtgtg
     2101 aatgatgttt tgttgttcag taataaaatc aaataaaaga gtgtttttaa cttcaataga
     2161 attcactttg gaaacatcac ttacttttag ataaatgata aatacatcat cttctatttc
     2221 tttttgtaca aattgaatct ctttaaattt tgcatttact ttaatggaaa aattatctga
     2281 aatatacttt ttaaattgtt ccaattgaac tgctgactct ttagaagaac ccaaatagaa
     2341 cttagtttta tgtttttttt ctaacgcatt attcaaatca tcaataaaaa accgagctgt
     2401 aatttgaatt actttttttt caggaacaaa attaatttgt gtcacagaaa cataaaattt
     2461 atgcatcgtg aaagaacata atcctataaa aaaaatagct ataaaaacaa tttttatctt
     2521 ttttctcatt taaattaatc cttaggaagg tttttatatt ctttgctttt actgattaaa
     2581 aagtctatta aatcaatttc tctatttaca gctaacagct ctttcacctt tgggtctgaa
     2641 tcacaaaatg ctaaaaacaa cccgatttca tctttgttta gttcaagtgt ttccgtaaaa
     2701 aagttatagg taaatttatc ttttactgct tcttgaaaat tctttgaaga ggtaaaaacc
     2761 atttctttgg gtttgtcttt tacgaaaatt tttccaatca tcttaccaat tttaacaaag
     2821 tccattccat aagtaattgt tccatcataa ataccggggt ttttaggagt agaactggca
     2881 tctttttcaa attgtaaatc agtaatttgt ccactatcaa aagctggatt catgtcggtc
     2941 actttcatat ttttaacatc tttgattaaa tcacctgtta acgtttttgg tgtcacgaca
     3001 acttcatcta attcattaat aaattcctct aatttgacat aaacaacttc aactttaaaa
     3061 tcaatctcag ctagaatcaa ctttttggat ttaaaaacta cactggaaaa aactaatgta
     3121 tcacttggtc tggcatacaa agtaaagtag cccaaatgat ctgaaattgc tcctttgtta
     3181 gaagaaacat taaaaacagt tacattatca atagacaatg attcagcaac aatttttcct
     3241 ttcaatacaa ctctaggaac aaattgtcca taggaaaatt gtactataaa aaaggtggct
     3301 attaaaagaa aattattttt cataagaatt taaatacgtt aaaaattctt gcgacaactc
     3361 acctaaaata aatgtggctc tcactttgtt ttttgaagtt aacgcttctg ctaagttaat
     3421 atcttctacc acataaaatt taaatccatt cacatattct tttggaatct taagcgaatt
     3481 tataaaaaat gcatcttcaa aataactttc caaacgaata attaatcgct ctcttttttc
     3541 aacttccagc tctcttttca aatttttcgt tctaccggaa acccaattta acaacggatc
     3601 taaaaaacct gttgttgccg ttctaattct tcgttcggct ggcgtataac ttaccggttt
     3661 gtaagtgata attcctaaat tttgtgcggt tattttagtg ttttcagaaa ctaccacttc
     3721 ctctaattga ttaactttag aagtcatgtt aatttctaca aaaccatcgt taatctcttt
     3781 tttagtgatg gtttttcgcc aataattgag atgaacagct gaaaaaacca ataaatcatc
     3841 ttcatttacc tccaaataaa attctccttt tgcattagta atcgttgctt tttcagaaac
     3901 taaatttaaa acatgaatcc catctaccga agcattttca actttaataa taccacgaat
     3961 ttgtttttca ttttgtgaaa acactttggc tgatgaaaaa atgaacaata aaaatagtac
     4021 cttattcata ttcttccatg tagattaaaa aatcagttgc cagtttcgag attcgaaaat
     4081 tgccctgtat tttattattt gactgtaaat aactcattaa ttcatcgtca taaattatat
     4141 aatatttaaa tccatcaaca aattcctttg gaattttcag ttgatttgca taaaactcat
     4201 tgtcattcca atcgttcaac ttccttaaaa gtagttcttt tttttcaatt ttcaaatcgt
     4261 ttttaagcat ttttgttcgt ccagttgccc aatacaataa tggatctaag ggtaatctaa
     4321 aagccatcag acctattaaa tccatttgtg ttattttagt tgggcgtaac tttcgttcgg
     4381 caggagtata tgctttaggt ttatagttaa ttatccctaa ctcttgtgca ttaattttgg
     4441 tgtattccgt taatacaact tcatttaatt tcacttcttt gggagtcatt atcacttcta
     4501 aaaatcctgt tttaaaatca t
//
LOCUS       DAOTCW010000169         4468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_15020_length_4468_cov_46.615681, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000169 DAOTCW010000000
VERSION     DAOTCW010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15020_length_4468_cov_46.615681"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="P5335_10640"
     CDS             <1..516
                     /locus_tag="P5335_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75379.1"
                     /translation="ERNIQLQHDSLQKKFDISAKDYNLEGVIFYNQKTFFFQRGNGPK
                     AQNGVFILNDDTKETNYHPIELPELKGVKATFTDAVLVDNQIYFLAAAEKSESTYLDG
                     EVTGTIIGKLSPETLEIEYTEKISDVHKFEGITLYQKNSNSLEFLLCEDKDSEDLKST
                     IYKLTIKKTTR"
     gene            complement(557..1048)
                     /locus_tag="P5335_10645"
     CDS             complement(557..1048)
                     /locus_tag="P5335_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRZ75380.1"
                     /translation="MKAKMFRALLPIAIVFTMISCSTSETEDAAAPIVENFSYTDVEL
                     DLMSKINEHRASVGLNTLQPINHISFKSAEHNDYMISNDVVGHQQFEQRANNIKQVLG
                     AVKVSENVAYNYQSNAGVLNAWLLSDGHKENIEGEYTHFGISIKTNPATGKKYYTNIF
                     MKR"
     gene            complement(1272..1916)
                     /gene="pdxH"
                     /locus_tag="P5335_10650"
     CDS             complement(1272..1916)
                     /gene="pdxH"
                     /locus_tag="P5335_10650"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRZ75381.1"
                     /translation="MEDLSNYRKSYEKSELLETTIPEDPINLFHRWFYETEEQSDTEE
                     VNAMTVSTIGLDGFPKARVVLLKKFYEEGFVFYTNYESEKGKAIEANPNICLSFFWHS
                     MERQVIIKGIAEKTTPSVSDGYFDARPDGSKLGAIVSNQSRVIASRTVLEEKLKELEQ
                     QFQGKEIPRPAFWGGFLVRPIEVEFWQGRPNRLHDRIRYQLQEDYFWKIERLAP"
     gene            complement(2011..2916)
                     /locus_tag="P5335_10655"
     CDS             complement(2011..2916)
                     /locus_tag="P5335_10655"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRZ75382.1"
                     /translation="MKLTILGCYAATPRTITNPTAQVLEIKNRMFLIDCGEGTQVQLR
                     KNKIKFSKINHIFISHLHGDHFFGLVGLISTFMLLNRESDLHVYGPKGIKEIILLQLR
                     YGNSFTGYNLFFHELQSKESVVLFEDEKVIVKTIPLNHRIYTNGFLFQEKNRERKLNI
                     DKIQNLEIDVVYYQKIKYGGDITLANGQVIPNSAITFEPEHEKTYAFCSDTVYDEQLI
                     PFIEGVDVLYHESTFLEKEMHLAEKTKHSTAKQAATMALKANVKQLVLGHFSTRYESI
                     NIFKEEAETIFPNVFLADDGKEFEF"
     gene            complement(2945..3277)
                     /locus_tag="P5335_10660"
     CDS             complement(2945..3277)
                     /locus_tag="P5335_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRZ75383.1"
                     /translation="MKVDQKGHTTILKNTQGDCLSFLEKINNQYNVFKNQNLILDLTF
                     DKTISLSNIKLFSDISKLHKKGKKSFVLVVDNVDIDKLPLKMTVVPTLMEAHDIIEME
                     EIERDLGF"
     gene            complement(3328..4254)
                     /locus_tag="P5335_10665"
     CDS             complement(3328..4254)
                     /locus_tag="P5335_10665"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRZ75384.1"
                     /translation="MSELSVNHLLGIKYLQPKDIDLIFETADHFKEVINRPIKKVPSL
                     RDITIANIFFENSTRTKLSFELAQKRLSADVISFSAAQSSVKKGETLIDTVNNILAMK
                     VDMVVMRHAHPGAAYFLSQNVKASIVNAGDGAHEHPTQALLDSFSIREKLGAVGGKKV
                     VIVGDILHSRVALSNIFALQMQGAEVKVCGPKTLIPKHIESLGVTVEPNLRKALEWCD
                     VANMLRVQNERMDVNYFPSTREYAQQYGVDKALLDSLNKEIVIMHPGPINRGVEITSD
                     VADSKQSVILEQVENGVAIRMAVIYLLASKIK"
     gene            complement(4259..>4468)
                     /locus_tag="P5335_10670"
     CDS             complement(4259..>4468)
                     /locus_tag="P5335_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRZ75385.1"
                     /translation="IQSFGRPSEIELLVLIDRRFSRHLPIQPDYRGRQVDAINNEKVK
                     VCWVENEGEDAVYLIPTITTNQPTT"
BASE COUNT         1402 a          805 c          735 g         1526 t
ORIGIN      
        1 gagcgaaaca tccaattgca acatgacagt ttacaaaaaa agtttgacat ttctgcgaaa
       61 gattacaact tagaaggagt tattttttac aatcaaaaaa cttttttctt tcaacgcggc
      121 aacggtccga aagcacaaaa cggtgttttc atattaaatg acgacaccaa agaaacaaat
      181 tatcacccga ttgaattacc cgaattaaaa ggtgtcaaag ctacatttac cgatgcagtt
      241 ttggtggata atcaaatcta ctttttagct gctgccgaaa aatcagagtc aacttattta
      301 gatggcgaag taaccgggac gattattggc aaactttctc ctgaaacgtt ggaaattgaa
      361 tatactgaga aaatttctga tgtccataaa tttgaaggca tcacactcta tcaaaaaaac
      421 agcaattctt tggaattttt actttgcgaa gataaagact cggaagacct gaaatcaact
      481 atttacaaat taaccataaa aaaaaccact cgataacgaa tggttcttct taggttaagg
      541 taaattcggg gcaaaattac cttttcataa aaatgttggt gtagtatttt tttccggttg
      601 caggatttgt tttgattgat ataccaaagt gagtatattc tccttcgata ttttctttgt
      661 gaccatcact taataaccaa gcgtttaaaa caccggcatt agactgataa ttgtaagcta
      721 cattttcact cacttttaca gctcctaata cttgtttgat gttattagct ctttgctcaa
      781 actgttgatg accaactaca tcattcgaaa tcatgtagtc attatgttca gctgatttaa
      841 aggaaatgtg attgataggt tgtaaagtat ttaacccaac actagctctg tgctcattga
      901 tttttgacat aaggtctaat tcaacgtcag tataagaaaa attctcaacg attggagctg
      961 ctgcatcttc tgtttcagaa gttgaacaag aaatcatagt gaacacgatt gcaatcggca
     1021 acaatgctct aaacattttt gctttcatag tagtaggtta agttttaaaa gtagatgtgg
     1081 ggcaaatacc tttatgttag tttaatcttt ttaaagctgt aaagtagatg tggggcaaat
     1141 atctttgtgc ttcagtaata agaacgtttc attttgtttg atgagtcaat attactacta
     1201 tgtcgttaaa gtgccaaata aaatcgatga aatacatctt tttttatttt aaaaatgttt
     1261 tttcttacat attaaggagc taatctttca attttccaga aatagtcctc ttgaagttga
     1321 taccttattc tgtcatgaag acgatttgga cgaccctgcc aaaactcaac ttctattggg
     1381 cgaactaaaa aacctcccca aaaagccggg cgaggaattt ctttcccttg aaattgttgt
     1441 tccaactctt tcaacttctc ttctaaaaca gttctggaag caattaccct actttgattg
     1501 gaaacgatgg ctcctaattt actgccatcc ggtcttgcat caaaataacc atctgaaaca
     1561 gaaggagttg ttttttctgc aattccttta ataataactt gtctttccat agaatgccaa
     1621 aagaaagaaa gacaaatatt cggatttgct tcaattgctt ttcctttttc agattcataa
     1681 ttcgtgtaga aaacaaatcc ttcttcataa aattttttaa gaagtacaac ccttgctttt
     1741 ggaaacccat ctaaaccaat agtagatacc gtcatcgcat tgacttcttc cgtatcactt
     1801 tgctcttcag tttcataaaa ccaccgatga aacaaattaa tgggatcttc gggaatggta
     1861 gtttctaaaa gctcactttt ttcgtatgat tttctgtaat tactaaggtc ttccattttg
     1921 ataggttcta aagtagtgag tggctaaggt acaaagaatt tgaagagctt gaaagagtaa
     1981 aaaaggatag taattctctt ttgcaagaaa ctaaaattca aattctttcc catcgtctgc
     2041 taaaaacaca ttgggaaaaa ttgtttcagc ttcttccttg aagatattta ttgactcata
     2101 tcgagttgaa aaatggccta gcaccaattg cttaacattt gcttttaaag ccattgtagc
     2161 cgcttgttta gccgtggaat gttttgtttt ttctgccaaa tgcatttctt tttctaaaaa
     2221 ggtactttca tgatataaaa catcgacccc ttcaataaaa ggaattaatt gttcatcata
     2281 aacggtatct gaacaaaagg cataagtttt ttcatgctcc ggctcaaaag tgatagctga
     2341 attcggaatt acttggccat ttgctaaggt aatatctcct ccgtatttga ttttttggta
     2401 atagacaaca tcaatttcga ggttctgaat tttatcaata ttgagtttac gttccctatt
     2461 tttttcctga aataaaaaac cattggtata aatacggtga ttcagtggaa tggtttttac
     2521 tattaccttt tcatcttcaa acagcacaac actttctttt gattgcagtt catgaaaaaa
     2581 caaattataa ccggtaaaag aatttccgta acgcaattgc agtaaaataa tttctttgat
     2641 gcctttcgga ccataaacat gtaaatcact ctcccgatta agcaacataa aagtggaaat
     2701 caatccaacc aatccaaaaa aatgatcgcc atgtaaatga gaaatgaaaa tatgattgat
     2761 tttagaaaat ttgattttgt tttttcttaa ttgcacctga gttccttcac cacaatcaat
     2821 caaaaacatg cggtttttta tttctaaaac ctgtgccgtt ggattagtaa tggtccgggg
     2881 tgtagcggca taacagccaa gaatggttag tttcatttag aagttgtccg ttttcggtta
     2941 tctgttaaaa ccctaaatcg cgttcaattt cctccatttc aatgatgtca tgagcttcca
     3001 taagggttgg aacaactgtc atttttaaag gcaatttgtc gatatccaca ttgtctacaa
     3061 ccaaaacaaa tgatttttta cctttcttat gcaatttaga tatatcactg aatagtttga
     3121 tattagataa ggatatggtt ttatcaaaag ttaaatctaa aatcaggttt tgatttttaa
     3181 acacattgta ctggttgttg attttttcca aaaaggaaag acaatcacct tgggtgtttt
     3241 taagaatggt cgtatgtcct ttttgatcaa ctttcattta taaagattct aaggtaataa
     3301 ggaactcagg ttctaagtta gtgaatttta tttaatttta gaagctaaaa gataaataac
     3361 tgccattctg atggctactc cgttttcaac ctgttctaaa ataaccgatt gttttgaatc
     3421 agccacatca ctggtgattt ctacaccacg attaattggg cccggatgca taattactat
     3481 ttctttgttc agcgaatcta aaagtgcttt atctacacca tattgttgag catattctcg
     3541 ggttgaagga aaataattca cgtccatccg ttcattttgt actcgtaaca tatttgcaac
     3601 atcgcaccat tctaaggctt ttcgcaaatt gggttctaca gtaactccta aactttcaat
     3661 gtgttttgga atcaacgttt ttggtccgca tactttcact tctgctcctt gcatttgaag
     3721 agcaaatatg tttgataacg ctactctgga atgaagaata tcacctacaa tcaccacttt
     3781 ctttccgcct actgcgccta atttctcccg gatggaaaaa ctgtctaaca acgcttgagt
     3841 ggggtgttca tgagctccgt cacctgcatt aacgatactc gcttttacat tttgagacaa
     3901 gaagtaagcg gctccggggt gagcatgtcg catcaccacc atatccactt tcatggctaa
     3961 aatattattt acggtatcga ttaacgtctc ccctttttta acagaagatt gagccgctga
     4021 aaaactaata acatcggcag aaagtctttt ttgagctaat tcaaacgata atttagttcg
     4081 ggtactgttt tcaaaaaaga tattggcaat ggtaatatcg cgtaatgaag gtaccttttt
     4141 tataggtctg ttgatgactt ctttaaaatg gtcagcagtt tcaaaaatca aatcaatatc
     4201 tttcggttga agatatttga ttcctaataa atgattgacg gatagttcag acattttttc
     4261 aggttgttgg ttggttggtt gttatcgtcg gaattaaata aactgcatct tcgccttcat
     4321 tttcaaccca acatactttt actttttcat tattaattgc atctacttgt cgccctctgt
     4381 aatcaggttg gataggtaaa tgccgactaa atcttcggtc aattagtacc aaaagttcta
     4441 tttcactagg cctaccaaaa gattgaat
//
LOCUS       DAOTCW010000170         4466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_15029_length_4466_cov_41.218091, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000170 DAOTCW010000000
VERSION     DAOTCW010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15029_length_4466_cov_41.218091"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3006
                     /locus_tag="P5335_10675"
     CDS             <1..3006
                     /locus_tag="P5335_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRZ75386.1"
                     /translation="EMSNRKITTILGKAIREGKYLNIYYKNQLGEIKPFWISILDIFG
                     DDKMVVDMFNVNKDEPILNCTLSISSIQSAEILKFSHYDVPEKLIKKILEDESLLVYE
                     FHRFDNTILNYYLECYKANSDPFLHQAYLLPEIDLTKLLQHSPFQLSLEQQQYLLKEI
                     YHNEYNKFQEYKLALSEFSIDMGSKGKFVVAYRELTYDPVKKTLLISKGTHFNPNFYI
                     KDVKYALSYYTDMSTADFEDFYLSDPTTTIEWLSENFKVGELPNTRPEVVVLGYSKID
                     IAPVFDQINTEQQNKEMQIPLKAFFKNASLLDRKNRKEPHIVLYDKNVNIDQLRTIYN
                     SLKYPITYVQGPPGTGKTQTILNIVVNCITNNKTILISSNNNIPIDGIKEKLIVGKYR
                     DKEIVLPFIRLGNKQCTLEALQTIKKLYEFETNDVPKESLLLNLKENSKERNKRLVEK
                     LQQYEERTDLQQNLEFIEGLLSRGDYYLLKLEKEKLEKKLHSIPVTKDEDVKGIFEII
                     EGNYKLLQFFYFESLKYIKRLKTKDYIELIEILHIEEENEQIKEFNNWLSDDKNLEKF
                     TKVFPIILTTNISSRRLGQKFKFDLLTIDEAGQCDIPTSLVPISKCKNMVLIGDTNQL
                     QPIILFDEHKNDKLMKQLNIDMDYSYYHHSILSAYKKMDSISREILLSYHYRCGKKII
                     EYSNKRFYEQRLNLSAIKENGSVQLLVVTNANQKHKNAQLEEAIEIVKYIKSNKLTDV
                     FILTPFRNQEEVLQHYVTKAKEAGVIADSVSCGTIHKVQGQENKTIIISTSLSTKTSR
                     RTYDWIKNNSQLINVGITRAKENLIVVTDLNAIDVLSRKDDDLYALIDYAAKNGTTHV
                     TQSTVNKLTIGFSNNSKFEDEFYKTMQHYCSLKDTRFRRNVKVVDIFPEEKDNPLVNK
                     KELDGVLFQNNQPKVVFELNGLEHYHHKKTMKSDALKMELLKKKQIQLLFIPNQYVKH
                     YEFIRELINKFNGDVYQKTLFDGYEEAKS"
     gene            complement(3284..4033)
                     /locus_tag="P5335_10680"
     CDS             complement(3284..4033)
                     /locus_tag="P5335_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HRZ75387.1"
                     /translation="MSIPSKKYFLFIDESGDHGLVTLDTSFPVFLLCGLLTSEDNYSD
                     LKNNINSIKNTFWGKKEVILHSRDIRKCEKEFQILFNLELKSEFYKRINQVIEEGKYR
                     ILASAINKEKYIKTYGKLSNDVYELALSFIIERAIFSLDEIKGITKQLEIVIEKRGKK
                     EDKKLEEHFQRLIARGTGFVSAERLEEVNLKITFKDKKENINGLQLADLVAYPIARYV
                     IEPKRANPAFEKLEPKIYKKNGKRYGLKIFP"
     gene            complement(4086..>4466)
                     /locus_tag="P5335_10685"
     CDS             complement(4086..>4466)
                     /locus_tag="P5335_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HRZ75388.1"
                     /translation="DGAWDNNTGLVEYQGINYKTKKVLFHLGPLEDGTINMVEFLAIV
                     HALALCKQNGWKHPIYSDSKIALGWIRDKKARTNHPPSEKNKKLFDMLARAEKWLEDN
                     TFENELLKWETKAWGENPADFGRK"
BASE COUNT         1570 a          833 c          702 g         1361 t
ORIGIN      
        1 gaaatgagca atagaaaaat cacaacaatt ttaggaaaag ccattagaga agggaagtat
       61 ttgaacatat actacaaaaa ccaactcggt gaaataaaac ctttttggat atctatttta
      121 gatattttcg gtgatgataa aatggtggta gacatgttta atgtcaacaa agatgagcct
      181 attctcaatt gcacgctttc tatatccagc attcaatcgg ctgaaattct gaaattctcc
      241 cactatgacg tgcctgagaa attaataaaa aaaatactgg aggacgaaag tttactcgtt
      301 tacgaatttc acagattcga caatacgatt ctaaattatt acttagaatg ttacaaagcc
      361 aatagcgacc cttttttaca ccaagcctat ttattaccgg aaattgattt gacaaagctg
      421 ctacaacata gtccatttca attgtcatta gaacagcaac agtacctcct caaagaaatt
      481 taccacaatg aatacaacaa gtttcaggag tacaaattgg ctcttagtga gttttccatt
      541 gacatgggct ccaaaggtaa atttgtagtg gcctatcggg agttgacgta tgatccggtg
      601 aagaagacgc ttcttatcag taaaggcaca cactttaatc ccaattttta tatcaaagat
      661 gtaaagtacg ctttatcata ttataccgat atgagtacag ctgattttga agatttttac
      721 ctaagcgacc ccactacaac cattgaatgg ttgagtgaaa attttaaagt aggggaatta
      781 cccaatacgc gacccgaagt agtggtgtta ggctattcca aaattgatat agctccggtg
      841 tttgatcaaa tcaataccga acaacaaaac aaggaaatgc aaattccgtt gaaagcgttc
      901 tttaaaaatg catccttatt agaccgcaaa aatagaaaag aaccccacat cgtgttgtac
      961 gataaaaatg tgaacattga ccagttacga accatctata attcactcaa atatccgatt
     1021 acgtatgtgc aaggccctcc cggaactgga aaaacacaaa ccatcttaaa cattgtagtc
     1081 aactgcatca ccaataataa aaccatactg atttcctcca ataacaatat tccgattgat
     1141 ggaatcaaag aaaaattaat agtaggaaag taccgcgata aagaaattgt tcttccattc
     1201 attaggttag gaaataaaca atgtacactg gaagccttgc aaaccatcaa aaagctatat
     1261 gaatttgaaa ctaacgatgt ccctaaagaa tcgcttttgc tcaatctaaa ggaaaattca
     1321 aaagagcgaa acaaacgatt ggtggaaaag ttacagcagt atgaagaacg aaccgattta
     1381 caacaaaatc ttgaatttat agaaggattg ttgtccagag gtgattatta tttattgaaa
     1441 ttagaaaaag aaaaactgga aaagaaatta cattctatac cggtaaccaa agacgaagat
     1501 gtaaaaggaa tttttgaaat cattgaagga aattacaagt tacttcaatt tttctacttt
     1561 gaatcattaa agtacatcaa gcgtttaaaa acgaaagact atattgaatt aattgaaatt
     1621 ctgcacattg aagaggaaaa tgaacaaatc aaagaattta ataattggtt gagcgacgat
     1681 aaaaacctcg aaaaattcac taaagtattt cctattattt taaccaccaa tatctccagc
     1741 cgtcgtttgg gacaaaagtt caaatttgat ttattaacca tagacgaagc cggacaatgt
     1801 gatattccaa ccagtttagt gcctatttct aaatgtaaaa acatggtatt gataggcgat
     1861 accaaccaac tgcaacccat tatccttttt gatgagcaca agaatgataa actgatgaag
     1921 cagctcaaca ttgatatgga ttacagttat taccatcact ctatactgtc ggcctataaa
     1981 aaaatggaca gcatttcaag ggaaatttta ttgagctatc attaccgctg tgggaaaaaa
     2041 attattgagt attccaacaa acgcttttat gaacaacgat taaacctctc ggctatcaaa
     2101 gaaaatggga gtgttcaatt gcttgtggtg accaatgcca accaaaagca taaaaacgca
     2161 caactggaag aagccattga aattgtaaag tacatcaaaa gcaacaaatt aaccgatgta
     2221 ttcattctta ctccgtttag gaaccaagaa gaagtgctcc aacattatgt aacaaaagca
     2281 aaagaagccg gagtgattgc tgactccgta agttgtggca ccattcacaa agtacagggc
     2341 caggaaaaca agaccatcat catctccacc tccctttcca ccaaaaccag ccgaagaacc
     2401 tacgattgga ttaaaaacaa cagccaattg attaacgtgg gcattacccg agcaaaagaa
     2461 aacttgattg tagtcaccga tttaaatgca attgatgtct tatcgcgtaa agacgatgat
     2521 ttgtatgccc taattgacta tgctgccaaa aacggtacaa cccacgtcac ccaaagtaca
     2581 gtgaataaac taaccattgg cttttccaac aattccaaat ttgaagatga attttataaa
     2641 accatgcaac actattgttc gctcaaggat acccgtttca ggcgaaatgt aaaagtggtg
     2701 gacatatttc cggaggaaaa agacaatccg ttggtgaata aaaaagaatt ggatggcgtg
     2761 ctctttcaaa acaatcaacc caaagtggtc tttgaattga atggattaga gcattatcac
     2821 cacaaaaaaa ccatgaaatc agatgctctc aaaatggagt tgttgaaaaa aaagcaaata
     2881 caattgctgt tcatccccaa ccaatatgtc aaacactacg agtttatccg tgaattaatt
     2941 aacaaattca atggcgatgt ctatcaaaaa acattgtttg atgggtatga ggaagcgaag
     3001 agttaaccca acaaccctca aaggtttcca acaaccctcg aagcgttcca aacccttcaa
     3061 aggttggttt ccaccccctt tgtcatgctg acgcaggaag catctctttt accaacccct
     3121 agtgctttac ttttagcaat gcttagtttt tctcgcttaa tattgaaatt aaaaaatatt
     3181 ttaatatatt tgcatcatca aattgataca caaaatcaaa gataaaagga ttggggtgtc
     3241 cccggtcgag cagaagccaa acattacgtt tggcttttgt tttttaagga aatattttaa
     3301 gtccatatcg tttcccgttt ttcttgtaaa tttttggttc caatttttca aaggctggat
     3361 tggcacgttt aggttctatg acgtatcgag caatcggata ggcaactaaa tccgcaagtt
     3421 gcaaaccatt gatgttttct tttttatctt taaaagttat tttcaaattt acttcttcca
     3481 atcgttccgc agaaacaaag ccggttcccc ttgcaatcag acgttgaaaa tgctcttcta
     3541 attttttatc ttcttttttc cctctttttt ctatgactat ttctaattgt ttagtaattc
     3601 ctttaatttc atccaagcta aaaatagctc tttctataat aaaggataaa gctaattcat
     3661 acacatcatt agataattta ccataagttt taatgtactt ttctttattt attgctgaag
     3721 ctaatatgcg atactttcct tcttcaatta cttggtttat tcttttataa aattcagatt
     3781 ttaattctaa gttaaataaa atttgaaatt ctttttcaca ttttctaata tctcttgaat
     3841 gtaaaataac ttcttttttg ccccaaaatg tattttttat cgagttgata ttatttttta
     3901 agtcactgta attatcctca ctagttaaca aaccacacaa taaaaacacc ggaaatgagg
     3961 tgtcaagcgt gactaagcca tgatcaccac tttcgtcaat gaataagaaa tatttcttag
     4021 aaggaatgct cattttttta aaattatata aatgatttta aaattttgat aaaagttgtt
     4081 taattttatt ttctcccaaa gtcagcagga ttttcacccc aagctttggt ttcccatttt
     4141 aataattcgt tttcaaaagt attgtcttcc aaccattttt ctgctctggc taacatatca
     4201 aacaatttct tgtttttttc actgggtgga tgattagttc ttgctttttt atctcttatc
     4261 caacctaaag caattttact atcagaataa ataggatgct tccatccgtt ttgtttacac
     4321 aaagctaaag catgtacaat agctaaaaac tcaaccatat taatcgttcc atcttctagc
     4381 ggacccaaat gaaacaaaac cttttttgtt ttatagttga tgccttgata ctctaccaaa
     4441 ccggtgttgt tatcccaagc accatc
//
LOCUS       DAOTCW010000171         4392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_15417_length_4392_cov_40.871801, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000171 DAOTCW010000000
VERSION     DAOTCW010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15417_length_4392_cov_40.871801"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="P5335_10690"
     CDS             complement(<1..920)
                     /locus_tag="P5335_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRZ75389.1"
                     /translation="MEKNFKMVAKTFFGFEDILAKEVQQLGGQDVQTGTRVVTFSGDK
                     GFMYKANLSLRTALKILKPIKWFKVFNDKSLYDVISSIDWSQFMNEKQTFVVDVTLYS
                     EAFNNSQFVALKTKDAIVDQFREKTGVRPSIDKDFPDLRIHIHLDRDQCTVSLDTSGE
                     SLHQRGYRSATNIAPINEVLAAGMLILSGWDGTSHFLDPMCGSGTILTEAAMIACNIP
                     ANINRKEFAFEKWNDWDNDLFDQIIDSLMKKTREFHYTIKGYDKAPSAVRKAQDNIQN
                     ANLEDYVTVEQKDFFETEKQTEGPLHMVFNP"
     gene            992..1756
                     /locus_tag="P5335_10695"
     CDS             992..1756
                     /locus_tag="P5335_10695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRZ75390.1"
                     /translation="MSESKNPETHNSQPATENWYASWFDTPYYHILYKDRDYDEAQAF
                     MDNLTHYLNLPEESKILDLACGKGRHSIYLNQLGYEVTGADLSENSINEASKSSNEKL
                     HFVKHDMRISFEEKFDAIFNLFTSFGYFEDDADNLTTLKAIRDSLTEYGFAVIDFMNV
                     DFVLENLIPEETKTVDGIDFHIKRYEKDNHIYKEISFSDKGKDYYFTEKVQALRLADF
                     EKMMDEAGIYLLDVFGDYKLRKFYKAQSERLILIFK"
     gene            1756..2487
                     /locus_tag="P5335_10700"
     CDS             1756..2487
                     /locus_tag="P5335_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRZ75391.1"
                     /translation="MHYIITFTAIILGFLIALFLKPQKKRNIKLLLAFSGAFLLSLTV
                     LHLLPELFEESHHVHEGEQVNSSIPVGVFIMIGILFQIILEFFSKGAEHGHVHVHTDE
                     NNELHHIPWLLFISLCIHALLEGFPIHENTNLAYGIAVHHFPIAIILTLFFVNAKMNK
                     FMVFVFMFSFALMTPLGTLLAEELHFAQHYSKEISAIVVGILFHISSTIIFESNEGHK
                     FNLAKVTMIVLGFLLAYVMEMGHSH"
     assembly_gap    2709..2808
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2809..2993
                     /locus_tag="P5335_10705"
     CDS             <2809..2993
                     /locus_tag="P5335_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6095 family protein"
                     /protein_id="HRZ75392.1"
                     /translation="GAIPLMFLGPVIIHNAFMNKHTNWHYLVLGIGIFICITSVSLMF
                     KGIQIIMKSLFDENSK"
     gene            3180..3548
                     /locus_tag="P5335_10710"
     CDS             3180..3548
                     /locus_tag="P5335_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV"
                     /protein_id="HRZ75393.1"
                     /translation="MKKLFPLLSLLFISCYQQERNCTDFKTGQFEFEQEIKGNKEISI
                     FERNDSLQIETFRGKTDTSSVRWINDCEFVLQKLHPKGMKEKKAIHMKILTTNEKTYT
                     FEYSFVGDSNKQKGIVTKVN"
     gene            3587..4357
                     /locus_tag="P5335_10715"
     CDS             3587..4357
                     /locus_tag="P5335_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRZ75394.1"
                     /translation="MEVFANPDAWVALLTLTFLEIVLGIDNIIFISIVTGKLPEEKRK
                     KATRLGLFLAMFMRIALLFGITLLIAMKAPWFSLDIGWFSGSFTGQAMVLFLGGLFLI
                     YKSTKEIHEKVDHKGEEEKDMKTSAAKSFGSVIVQILLIDLIFSVDSILTAVGMTNGI
                     EGALYIMVTAVVISVGVMMLFAVPVGNFVNANPSIQILGLAFLILIGFMLITESMHLS
                     NATLVGQHVGAVPKGYLYFAIAFSLAVEFINMKIRKKK"
BASE COUNT         1425 a          699 c          697 g         1471 t
ORIGIN      
        1 ggattgaaaa ccatatgaag cggaccttcg gtttgttttt ccgtttcgaa aaagtctttt
       61 tgttccacgg tgacataatc ttctaaattg gcattttgaa tattgtcttg agctttccga
      121 acagcactcg gagctttatc gtaacctttg atggtgtaat gaaattcacg agtttttttc
      181 atcaaactat caataatttg atcaaataaa tcattgtccc aatcattcca tttttcaaaa
      241 gcaaactctt tacggtttat attagctgga atattacacg caatcatcgc tgcttctgtt
      301 aaaatagttc cgcttccgca catcgggtca agaaaatgag aggtcccatc ccaacccgaa
      361 agtatcaaca ttcctgcggc taaaacttca ttaattggag caatattggt tgctgaacgg
      421 tagccacgtt ggtgcaacga ttcacctgaa gtatccaacg aaaccgtaca ttggtcacga
      481 tctaagtgaa tatgaattcg caaatccgga aaatctttgt caatactcgg acgaacaccg
      541 gttttttctc gaaattgatc tacaattgca tctttcgttt ttagtgctac aaattgtgag
      601 ttgttaaatg cttccgaata caaggttaca tctactacaa aagtttgctt ttcattcata
      661 aattgactcc aatcaatact tgaaattaca tcatataaac ttttgtcatt aaacacttta
      721 aaccatttaa ttggtttcaa aattttaagt gctgttctca acgataaatt agctttatac
      781 ataaaacctt tatcaccgga aaaagtaaca acccgtgttc cggtttgcac atcttgtccg
      841 cctaattgct gaacttcttt tgctaaaata tcttcaaaac caaaaaaagt tttggcaacc
      901 atcttaaaat tcttttccat gtctttttaa atctgtaaaa cttggcaaaa atacactaaa
      961 tttgcacgtt cttaaagcaa ctgttcaatt aatgtcagaa tctaaaaatc ccgaaaccca
     1021 caactcgcaa cctgcaacag aaaactggta cgcttcgtgg tttgacacac cctattatca
     1081 cattctttat aaagatcgag attatgatga agcacaggct tttatggata atctcaccca
     1141 ttatttgaat ttaccggaag aatctaaaat tttagattta gcttgtggaa aaggacgtca
     1201 ttcgatttat ttgaatcaat tgggttatga agtaactgga gcagatttgt ctgagaatag
     1261 catcaatgaa gcttcaaaat catccaatga aaaattgcat tttgtaaaac acgacatgcg
     1321 aatttcattc gaagaaaaat tcgatgccat tttcaattta tttaccagtt ttggttattt
     1381 tgaagatgat gctgataatt taacgacgct aaaagctatt cgcgatagct taactgagta
     1441 tggctttgct gtgatagact ttatgaatgt agattttgtg ttagaaaatt taattcccga
     1501 agaaacaaaa acggttgatg gaatcgattt tcatatcaaa cgctatgaaa aagataatca
     1561 tatttacaaa gaaatttcgt tttcggataa aggaaaagat tattatttta ctgaaaaagt
     1621 tcaagctctc cgattagcag attttgaaaa aatgatggac gaagccggca tttatttatt
     1681 ggatgttttt ggagattata aactccgaaa attttacaaa gcacagtctg aacgtttaat
     1741 tttgatattc aaatgatgca ttatattatt acttttaccg ctattattct tggtttttta
     1801 atcgctttgt ttttaaaacc acagaagaaa agaaacatca aattactgtt ggctttcagt
     1861 ggtgcttttt tactttcgtt aaccgtttta catttattac ctgaactatt cgaggaatcg
     1921 catcatgtgc atgagggcga acaggtcaat tcatccattc cggtaggtgt ttttataatg
     1981 attgggattt tgttccaaat tatcttagaa tttttctcca aaggtgctga acacgggcac
     2041 gttcatgtgc ataccgatga aaataatgaa ctgcatcaca ttccttggtt gttatttatt
     2101 agcttgtgta ttcacgcttt gttagaaggc tttccaattc atgaaaatac aaatttagcc
     2161 tatggaattg ctgtacacca cttccctatt gccataattt taaccttgtt ttttgtgaat
     2221 gcaaagatga acaaattcat ggtttttgtc tttatgtttt cgtttgcttt gatgacacca
     2281 cttggaacct tattagcaga agaattgcat tttgctcaac attattccaa agaaatttcg
     2341 gctattgtgg ttggaatatt attccatata tcttcaacaa ttatttttga aagtaatgaa
     2401 ggtcacaaat tcaatttggc aaaagtcact atgattgtgt tgggattttt attggcgtat
     2461 gtgatggaaa tgggacattc gcattaaaat ttcaaaaatc aacttcaaaa atcaacttca
     2521 attacaaaat tcaaattcaa aatttgcaac tttgcgactt tacgagaaac gaaaatctct
     2581 tatctttgag tgaaattttg cactatgtct tttaccaaaa ccaccgaaca atcctccaaa
     2641 tacgagcatc tggaaaaaat gtcagtttct gaacttttac aaaacatcaa taccgaagat
     2701 aaaactgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt ggagcaattc
     2821 cattgatgtt tttaggaccc gtaattattc ataatgcgtt tatgaataaa catacaaact
     2881 ggcattattt ggttttagga atcgggattt ttatttgtat tacatctgtt tctttgatgt
     2941 ttaaaggaat tcaaattatt atgaaaagct tatttgatga aaactcgaaa taatgggaac
     3001 ctctcaattt tatacctaca tgggagctat tatttattgg gtcttaatca aattctgtca
     3061 atcaaaactg agagatgaaa tgtcagagaa aaataatttt cgaaatctac tattggcagg
     3121 agtttttaat tttctagtta cattattaat catcttattc tttgtttata attaaaaata
     3181 tgaaaaaatt attcccacta ttaagtttac tattcatctc ctgttaccaa caagaaagaa
     3241 attgcaccga ttttaaaacc ggtcaatttg aatttgaaca agaaattaaa ggaaataaag
     3301 aaatatccat ttttgaacga aatgattctc tacagataga aaccttccgt ggaaaaacag
     3361 atacctcctc tgttcgttgg ataaatgact gtgaatttgt gttacaaaag cttcatccta
     3421 aaggtatgaa agaaaaaaaa gctattcaca tgaaaatttt aaccactaac gaaaaaacct
     3481 atacctttga atattctttt gttggcgata gtaataaaca aaaaggaatt gtaacaaaag
     3541 taaattaatt acgtaaaaga atcgttaacc aaattaaaac tgacttatgg aagtattcgc
     3601 aaatcccgat gcatgggttg ctctattaac acttaccttt ttagaaattg ttctaggaat
     3661 cgataatatc attttcatct ctattgttac aggaaaatta cccgaagaaa aacgtaaaaa
     3721 agcaacacga ttaggattgt ttttagccat gtttatgcga attgcattgt tatttggaat
     3781 cacgctttta atagcaatga aagctccttg gtttagtttg gatattggtt ggttcagcgg
     3841 tagttttacc ggtcaggcaa tggtactatt tttaggtgga ttatttctta tttataaaag
     3901 taccaaagaa attcacgaaa aagtagacca taagggagaa gaagaaaaag acatgaaaac
     3961 ttctgcagcc aaatcgttcg gtagtgtgat tgttcaaatt ctattaattg acttgatttt
     4021 ctctgtagat agtattttaa ccgcagttgg tatgactaac ggcattgaag gagctctcta
     4081 catcatggtt actgccgtag taatttcagt aggtgtcatg atgctttttg ctgttccggt
     4141 tggaaatttt gttaacgcaa atccatccat tcaaatcctt ggtttagcat tcttaatatt
     4201 aattggattt atgttaatta cagaaagtat gcatttgtct aatgcaacat tagttggtca
     4261 acatgtagga gctgtcccga aaggatatct ttattttgct attgcttttt cattagcagt
     4321 tgagtttatt aacatgaaaa ttagaaagaa gaaatgacat tcttactatt aaaaaataaa
     4381 ttataaaaaa aa
//
LOCUS       DAOTCW010000172         4335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_15718_length_4335_cov_44.379673, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000172 DAOTCW010000000
VERSION     DAOTCW010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15718_length_4335_cov_44.379673"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="P5335_10720"
     CDS             <1..276
                     /locus_tag="P5335_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRZ75395.1"
                     /translation="GNVRSKRSKMLRGLSVKKRRAFYESQIGTTRTVLFEAENKEGYI
                     HGFTENYVKVKTPWNPELVNTLHEIKLTKIDEDGSVRMEFVRDLISV"
     gene            complement(284..910)
                     /locus_tag="P5335_10725"
     CDS             complement(284..910)
                     /locus_tag="P5335_10725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRZ75396.1"
                     /translation="MKCPLCNHPSNHFYSQKSREFYFCTKCFSVFLDDKNYISETEER
                     KHYEMHNNDVNDIRYQSFVSPIVDSILNDFKKTDKGLDFGSGTGPVITKMLRDNDYDI
                     QEYDPFFADNSETLKQKYDYIACCEVIEHFHHPKKEFELLFNLLKPGGKLYCKTDLFT
                     EVKNFDSWGYKNDPTHVFFYHQKSILWIQKNIGFTSVKIDGRLIVFEK"
     gene            complement(913..1194)
                     /locus_tag="P5335_10730"
     CDS             complement(913..1194)
                     /locus_tag="P5335_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRZ75397.1"
                     /translation="MEIKDNLLLRHFETPTEFGLLVMEYAIQERKIFLTKLNQPENLP
                     NEYIEDFIREVLSIAVERKFKVVPTHPKLAHFFRKNPAYKELLPPGIKL"
     gene            1403..2065
                     /locus_tag="P5335_10735"
     CDS             1403..2065
                     /locus_tag="P5335_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRZ75398.1"
                     /translation="MEKIPVYFMPGLAASTTIFERIHLPEDIFEIFFLEWVIPIKNET
                     LVMYASRLAKEIVHENPVLIGVSFGGVLVQEMAKIIPVRKTIIISSVKSNQEFPRRMK
                     IAKATKAYKLLPTGLVHNVEGLAKFAFGDSINQRLKLYEKYLSVRDKSYLDWALESII
                     LWDRTIEDKNVVHIHGDADDVFPIKYISNCHVVKGGSHIMILSKYKWLNENLPKIILN
                     EL"
     gene            2116..3003
                     /locus_tag="P5335_10740"
     CDS             2116..3003
                     /locus_tag="P5335_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRZ75399.1"
                     /translation="MKRINQIIIVLVLFGLSGIFIYSTSKKTMVTDKVEAYALPNQID
                     FAGESTPLNLVDVRERLDREMLINTNLHSTTTLILKRANRVFPVIEPILKKYGVPDDF
                     KYLAVIESGLVNVVSPAGARGVWQFMPETAKEKGMEVNDLVDERYHLEKSTEAACRYL
                     LDAKNKFGSWTLAAAAYNGGMGGINKQMESQQVGNYYDLLLTEETSRYVFRILALKEI
                     MKNPVKYGFDIPNEQLYFPIEMKKVQVDSSITNLTSFAKNMGINYKILKLHNPWLRDK
                     KLDNKSRKVYTLEIPTEGY"
     gene            3021..3386
                     /locus_tag="P5335_10745"
     CDS             3021..3386
                     /locus_tag="P5335_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRZ75400.1"
                     /translation="MTTTTLILLLCIGLLAGVLSGLIGIGGGIIMIPLLLLLGLSQHQ
                     AQGTSLAVLAVPVTALAAFNYYKEGYVDWKYAALIAVFFVIGGYIGSKFALQIDEKVL
                     KKIFGFVLLLVAGKLIFGK"
     gene            complement(3389..4081)
                     /locus_tag="P5335_10750"
     CDS             complement(3389..4081)
                     /locus_tag="P5335_10750"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRZ75401.1"
                     /translation="MKNLLVILLFLGQFFPLFGQENERLFSDAIRTNFSKFKQQSNKA
                     YKSGDFERGQFLFDSLVQNQLIGSKFNDFTLKRVSDRKLKISKVKKPIFLVTYAAWCV
                     PGKGEILALNKLAKEFHKEIEFVVIVWGKKQEVKKFSKKISGQIEICYAHKNYKNDNE
                     IVENMKETLGFPTCYLIDSNLQIIDIKRGAASVPLKTPNKKAYEMNYAVFKERIYQLT
                     SIQNKKDFLVNQ"
BASE COUNT         1448 a          600 c          754 g         1533 t
ORIGIN      
        1 ggaaatgtac gttcgaaacg cagcaaaatg cttcgtggat tgtctgttaa aaaaagaaga
       61 gcattctacg aaagccaaat cggaactact cgaacggttt tatttgaagc cgaaaacaaa
      121 gaagggtaca ttcacggttt taccgaaaat tatgtgaaag taaaaacccc ttggaatccg
      181 gaattggtga atacgttaca cgaaatcaaa ttgacaaaga ttgatgaaga tggttcggta
      241 aggatggagt ttgtgaggga tttaatttct gtataatacg tttttacttc tcaaatacaa
      301 tcaatcgtcc gtcaattttc acacttgtaa aacctatatt tttctgaatc cataagatag
      361 atttctgatg gtagaaaaaa acgtgcgttg gatcgttttt atagccccag gaatcgaagt
      421 ttttgacttc agtgaataaa tcggttttgc agtacaattt ccctcccggt tttaaaagat
      481 tgaaaagtaa ttcaaattct tttttgggat ggtgaaaatg ctcaatcact tcacaacagg
      541 caatgtaatc gtatttttgt tttaaagttt ccgagttatc cgcaaaaaag ggatcatatt
      601 cctgtatgtc atagtcatta tcacgtaaca tttttgtgat taccggtccg gttccggaac
      661 caaagtccaa acccttatct gtttttttga aatcgttaag aatactgtct acaattggag
      721 aaacaaatga ttggtaacga atgtcattca catcattgtt atgcatttca taatgttttc
      781 tttcctctgt ttctgaaatg tagtttttat catctaagaa tacggaaaag catttggtgc
      841 aaaaataaaa ttcacgcgat ttttgactgt agaaatgatt agaaggatga ttacaaagtg
      901 ggcatttcat tattataact taattccagg aggaagtaat tctttataag caggattttt
      961 tctgaaaaag tgagccaatt tagggtgagt tggaaccact ttaaactttc gttctaccgc
     1021 aatggaaagt acttctctta taaaatcttc aatgtattca tttggtaaat tttcaggttg
     1081 attgagtttt gtcaaaaata tttttcgttc ctgaatagca tattccatta ccaataatcc
     1141 aaattctgtt ggtgtttcaa aatgtcttaa gagtagattg tctttaattt ccatggtatt
     1201 tcaattttaa tcgataaaag catttagaaa agatacatgt cgaataaaaa tgcattacaa
     1261 atgtacggtt tttaattttt ttagggatta gcaatgccgg taaatctcaa acatttaaca
     1321 tttaaggact attatttaat actaatttgt actgattttt agagattagc cttaatttta
     1381 aacccattaa ttgaattcac caatggaaaa aattccggtt tattttatgc ctggtttagc
     1441 ggccagtaca acaatttttg aacggataca tttgcctgag gatatttttg aaatcttttt
     1501 tttggagtgg gtaattccaa taaaaaatga aactttggtg atgtacgcca gccgattagc
     1561 aaaggaaatt gttcatgaaa atccggtatt aattggcgta tcttttgggg gcgttttagt
     1621 tcaggaaatg gcaaaaatta ttcctgttcg aaaaacgatt attatttcca gtgtcaaatc
     1681 gaatcaggaa tttccaaggc gaatgaaaat tgcgaaagcc actaaagcat ataaattgtt
     1741 gcccaccgga ttagtccaca atgttgaagg tttggcgaag tttgcttttg gcgatagtat
     1801 caatcaaagg cttaaattat atgagaaata tttgagtgta agagataaat cctatttaga
     1861 ttgggctttg gaatcaataa ttttgtggga tagaaccatt gaagacaaaa atgttgttca
     1921 tattcacggt gatgcggatg atgtttttcc aataaaatat atttccaatt gtcacgttgt
     1981 caaaggagga tctcatatta tgatattgag taaatacaaa tggttgaatg aaaatttgcc
     2041 aaaaattatt ttaaatgaat tatgagttgg attgattttt gtagttttac cataaccaat
     2101 aaaaaaaaat agaaaatgaa aaggattaat caaattataa ttgtgctcgt tttattcgga
     2161 ctgagtggaa tatttattta ttcaacttcc aagaaaacaa tggttacgga taaagttgaa
     2221 gcgtatgctt tacctaatca aatcgatttt gcaggtgaat ctaccccttt aaatttagta
     2281 gacgttcgtg agcgattgga tcgcgaaatg ttgattaata ctaatttaca ttctactact
     2341 acactaatat tgaaaagggc aaatagagta tttcctgtaa ttgaaccaat tttgaaaaaa
     2401 tatggtgttc cggatgattt taagtattta gctgtaattg aaagtggctt agtaaatgtg
     2461 gtttcccctg cgggagcaag aggtgtgtgg cagtttatgc ctgaaacggc gaaagaaaaa
     2521 ggaatggaag taaacgactt ggttgatgaa cgatatcatt tagaaaaatc aactgaagct
     2581 gcttgtcgct atttattaga tgcgaaaaat aaatttggca gttggacatt agctgcagct
     2641 gcttataatg gagggatggg cggcattaat aaacaaatgg aaagccaaca agttggtaat
     2701 tactatgatt tgttattaac agaagagact tcccgttatg tttttcggat tttggcttta
     2761 aaagaaatta tgaaaaatcc tgtaaaatat ggttttgata ttccaaatga gcaactgtat
     2821 ttcccaattg aaatgaaaaa agttcaagtt gatagttcta ttacaaattt gacttctttc
     2881 gctaagaata tgggaataaa ttataaaatt ttaaaactac acaatccgtg gttacgagat
     2941 aaaaaattag ataataaatc tagaaaagtc tacacattgg aaattccaac agagggttat
     3001 taattatttt acaaatgaaa atgactacaa caacccttat tctattgctt tgtattggtc
     3061 ttttagctgg tgttttaagt gggttaattg gtattggtgg tggcattatt atgattccct
     3121 tattattgct tttaggttta tcacaacacc aagcacaagg cactagtttg gcagttttgg
     3181 ctgttccggt gactgcttta gcggcattta attattataa agaaggctat gtcgattgga
     3241 aatatgctgc tttaatagct gttttttttg ttattggagg ttatattggc agtaaatttg
     3301 cccttcaaat agatgaaaag gtgctaaaaa aaatatttgg ttttgtactc ttattagtgg
     3361 ccggaaaatt aattttcgga aaataagttt attgatttac taaaaagtct tttttgtttt
     3421 gaatacttgt taattgataa atacgttctt taaatacagc ataattcatt tcatacgctt
     3481 ttttattagg tgtttttaaa ggaaccgaag cagcacctct tttaatatca attatttgta
     3541 aattggaatc aattagataa cacgttggaa aacctagtgt ttccttcatg ttttcaacta
     3601 tttcattatc atttttatag tttttgtggg cgtaacaaat ttcaatttga ccacttatct
     3661 ttttggaaaa ttttttaact tcttgttttt taccccatac aatgactaca aattctattt
     3721 ccttatgaaa ttctttagcc agtttattta acgctaaaat ttctcctttt cccggaacac
     3781 accaagcagc ataagtaact aaaaaaattg gttttttaac tttactaatt ttaagttttc
     3841 tgtcagaaac cctttttaaa gtaaaatcat tgaatttgga acctattaat tgattttgaa
     3901 ctaagctgtc aaataaaaat tgtcctcttt caaaatcacc ggatttatag gctttgttac
     3961 tttgctgctt aaattttgaa aagtttgtac ggattgcatc ggagaagagt cgctcatttt
     4021 cttgtccgaa aagagggaag aattgaccta aaaataataa tatgactaat aaatttttca
     4081 tctgattagt atattaacaa ctcaaatgta taaaaacttt tcaacaaatc catatttttt
     4141 tcaaatatct cattgataat tagagtaatg tcaaatattt gtcttcaatt aagttttaag
     4201 ttggactgtt tttaattaca aaaatagcaa tccaacattt tttgttttcc atcatcatag
     4261 ctcaaattat cactgaaatt tttgaaaaaa ttaaatttat agtaaaatag aaatcattaa
     4321 attatactaa taagg
//
LOCUS       DAOTCW010000173         4325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_15779_length_4325_cov_36.147073, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000173 DAOTCW010000000
VERSION     DAOTCW010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15779_length_4325_cov_36.147073"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="P5335_10755"
     CDS             complement(<1..571)
                     /locus_tag="P5335_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75402.1"
                     /translation="MKQIVLIISFIILISCQENEPFTFLTGKINGTDAKEIELEGINF
                     SKKIPLQANGTFSDTLDLPYDGMYYLILTDEKSFYVYLEKGFQLQLETKADDFINSIK
                     YSGLGQDENNYLIQKNKVIESKYGDQSNFENVLKIYSDEEQVFLDNIESLKKSMLDKL
                     ATSYIKNERFIKNEKKEIDILCLKYYEQYS"
     gene            691..2187
                     /gene="hutH"
                     /locus_tag="P5335_10760"
     CDS             691..2187
                     /gene="hutH"
                     /locus_tag="P5335_10760"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRZ75403.1"
                     /translation="MNYHYISSDLLTIESINEILLEHKLLALSEEAKVNIQKCRDYLD
                     VKMQSQTDPIYGINTGFGSLCNVKISNENLSQLQENLVKSHACGTGEEVPQEIVKLML
                     LLKIQSLSYGHSGVQLETVERLIEFYNHEILPVIYTQGSLGASGDLAPLAHLSLPLLG
                     EGEVYFESEKVHSKKVLAHFGWIPIVLQSKEGLALLNGTQFMSAYGVYVLLKSYKLSY
                     LADLIGTVSLEGFDGRIDPFNELIHLVRPHKGQIETAQRITEFLEGSEIIKQYKTHVQ
                     DPYSFRCMPQVHGASKDAMDYARRVFKTEINSVTDNPNIFIGEDLIVSGGNFHGQPLA
                     LALDFLGIALAELGSISERRTYQLISGFRGLPAFLVDNPGLNSGFMIPQYTAASIVSQ
                     NKQLATPASIDSIVSSNGQEDHVSMGANAATKCLKIAENLERILAIELMNASQAIDFR
                     RPLKSSDFIELFLKSYRSEVSFVKEDRILHYDIENSITFLKSFEIEME"
     gene            complement(2226..3596)
                     /gene="rimK"
                     /locus_tag="P5335_10765"
     CDS             complement(2226..3596)
                     /gene="rimK"
                     /locus_tag="P5335_10765"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HRZ75404.1"
                     /translation="MLDNKLILGSEEWCSFPELGIPTIKARVDSGAKTSALHAINISP
                     FIKEGEHWVKFDINPIQNNVKTIIHCQAPLIDKRIVKSSSGFREQRFVIQSQLKLGES
                     SWTIEMTLTNRDSMGFRMLLGREAMSGRAMVDPERKYLLGDNSTDKLKDLYPDVLKEK
                     SGLRIGLLASNPELYSNKRIMEAGERRGHEMQFLNIKECYMKLDADTPEIHYRGGKIL
                     NELDAVIPRIRPNITFYGCALTRQFEALKIFCLNSSAAITQSRDKLYSLQLLLNNGID
                     IPTTGFANSPLDTDDLIKMVGGTPLIVKLLEGTQGKGVVLAETKKAAESVINAFKSLN
                     ANILVQEFIKEANGKDLRLFVIDGKVVATIQREALAGEFRANIHLGGTASIIKPTKEE
                     KIIAVKAAKAMGLKVAGVDIIRSSKGPLLLEVNSSPGLEGIEGATNKDIAGEMIKAIE
                     KNFKVK"
     gene            complement(3688..>4325)
                     /locus_tag="P5335_10770"
     CDS             complement(3688..>4325)
                     /locus_tag="P5335_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRZ75405.1"
                     /translation="RRKPYCVVLLDEIEKAHPDVFNMMLQVLDDGYMTDSLGRKIDFR
                     NTIIIMTSNVGARQLKDFGQGVGFGTAAKVSQADEHSKSVVENALKKTFAPEFLNRID
                     DVIVFNALEKEDINLIIEIELKKLYSRIKDLGYSLTLTDKAKAFIAEKGFDKQFGARP
                     LKRAIQKYVEDSLAEEIITSKIGDGDEIFMDIEDDAQELNVKVKKAEKPTS"
BASE COUNT         1370 a          730 c          690 g         1535 t
ORIGIN      
        1 gggaatattg ttcgtaatat tttaaacata aaatgtcaat ttcttttttt tcatttttga
       61 taaatcgttc attttttatg taagatgtag ccaatttatc aagcatactt tttttaaggc
      121 tctcaatatt atctaaaaaa acttgttctt catccgaata gattttcaaa acattttcaa
      181 agttagattg gtcaccatat ttagattcta tgactttatt tttttgaatc aagtaattgt
      241 tttcatcttg acccaaaccg gaatatttta tcgaattgat aaaatcatca gctttggttt
      301 ccaattgaag ttgaaaacct ttttctaagt acacataaaa tgatttttca tccgtcaaaa
      361 tcaaataata catgccgtca taaggcaaat ccaaagtatc tgaaaaggtg ccattggctt
      421 gaagaggaat ttttttggaa aaattgattc cttccaattc aatttctttt gcatcagtac
      481 cattaatttt tccggttaaa aaagtaaatg gttcattttc ttggcaagaa attaaaataa
      541 taaagcttat aattaaaaca atttgtttca tagtataggt gttatttctt caaaaatagg
      601 aattcaattt taattgttat taaataatca ataattgttt tgcttttatg caaacgtttg
      661 cggaattttg aaacctcaat taatcaatag atgaattacc attatataag ttccgattta
      721 cttacaatag agtccataaa cgaaatttta cttgaacata aactactcgc tttatcggaa
      781 gaagcaaaag ttaatattca aaaatgccgt gattatttag atgtaaaaat gcaatcgcaa
      841 acggatccga tttatggaat caataccggt tttggatcgt tatgtaatgt taaaatatcg
      901 aatgaaaatc tttcacaact tcaagaaaac cttgtaaaat cacatgcttg tggtacgggt
      961 gaagaagtac ctcaagaaat tgtaaaattg atgttgttac tcaaaattca atcgttgagt
     1021 tacggacatt caggagtaca attagaaacg gtggaacgtt tgattgagtt ttataatcac
     1081 gaaattttac cggtaattta tactcaaggt tcattaggag cttccggaga tttggctcca
     1141 ttagctcatt tgagtttacc tttattaggg gaaggtgaag tttattttga aagtgaaaaa
     1201 gtgcattcta aaaaagtttt agcacatttt ggatggattc caattgtttt acaatctaaa
     1261 gaaggtttgg cattgttgaa tggcacccag tttatgagtg cttatggcgt ttatgtttta
     1321 ttgaaatctt ataaattatc ctatttagca gacttaatcg gaactgtttc gttggaaggt
     1381 tttgatggaa gaattgatcc ttttaacgaa ttaattcact tggttcgacc tcacaaaggt
     1441 caaattgaaa cggctcaacg aatcactgag tttttggaag gtagtgagat aattaaacaa
     1501 tataaaacac acgttcaaga cccatattca tttcgttgca tgccacaagt tcacggagct
     1561 tctaaagatg caatggatta tgcacgacgc gtatttaaaa ctgaaatcaa ttcagttact
     1621 gataacccta acatatttat tggtgaagat ttaattgttt ccggtggtaa ttttcatgga
     1681 caaccgttgg cgttagcctt agatttttta ggaatagctt tggcagaatt gggaagtatt
     1741 tccgaaagaa gaacttatca attaatttca ggatttcgcg gtttacctgc atttttggtt
     1801 gataatccag gattaaattc cggttttatg attccgcaat ataccgcagc tagtattgta
     1861 agtcagaata aacaattggc cactcctgct agtattgata gcatcgtttc cagtaatggt
     1921 caagaagatc atgtaagtat gggagcgaat gcagcgacca aatgtttaaa aatagcggaa
     1981 aatttggaac gtattttagc tatcgaatta atgaatgctt cgcaagcgat tgattttcga
     2041 agaccattga aatcgagtga ttttattgaa ttgtttttaa aatcgtatcg ttcagaggtt
     2101 tcttttgtaa aagaagatcg aattttgcat tatgatattg aaaactcgat tacttttttg
     2161 aaaagttttg agattgagat ggagtaaaaa aaatcccaaa aacttttaaa cttttgggat
     2221 tttttttatt tcaccttaaa attcttctca atcgctttaa tcatttcgcc ggcaatatct
     2281 ttgttcgttg caccttcaat tccttctaaa ccgggagagg aatttacttc taataaaagc
     2341 ggacctttac tggagcgaat aatatccact ccggcaactt ttaatcccat ggctttagca
     2401 gctttaacag caataatttt ttcttcctta gttggtttaa taatagaggc tgttccaccc
     2461 aaatgaatat tagctctgaa ttcaccagcc aatgcttccc gttgaatagt ggctactact
     2521 tttccgtcaa tcacaaacaa tcgcaaatct tttccgttgg cttccttaat gaattcttgc
     2581 actaaaatat tcgcattcaa acttttaaat gcattaatta cactttctgc tgcttttttg
     2641 gtttcggcta aaacaactcc ttttccttgt gttccttcta aaagtttaac aatcaaagga
     2701 gttccaccaa ccatttttat caaatcatct gtatctaggg gagaattggc aaaacctgtt
     2761 gtcggaatat caattccatt atttaataac aattgtaacg aatataattt atctcgcgat
     2821 tgtgttatgg cagccgaaga attcaaacaa aatatcttta aagcctcaaa ttgtcgagtc
     2881 aatgcacaac cataaaacgt tatattcgga cgaattctcg gaattacagc atccaattca
     2941 tttaaaattt ttccgcctct gtaatgaatt tcgggcgtgt cggcatctaa tttcatgtaa
     3001 cattctttga tgtttaaaaa ttgcatttcg tgtccgcgac gttcaccggc ttccatgatt
     3061 cgtttgttac tgtataattc aggattactg gctaataatc caattcggag tccgcttttt
     3121 tcttttaaca catccggata taaatctttt aatttatcgg tagaattatc ccctaataaa
     3181 tattttcgtt ctgggtcgac cattgctcgt ccactcatgg cttcacgacc caataacatt
     3241 cggaaaccca ttgaatctcg attggttaaa gtcatttcga tggtccaact ggattctcct
     3301 aattttaatt gggattgaat gacaaaacgc tgttctcgaa atccgcttga actttttaca
     3361 attcgtttat caattaatgg tgcttggcaa tgaataatgg ttttgacatt gttttgaata
     3421 ggattaatgt caaattttac ccaatgctct ccttctttaa taaacggact aatgttgatg
     3481 gcatgaagtg cagaagtttt tgcaccggaa tccactcgag ctttaatggt tggtatacct
     3541 aattcaggaa aggaacacca ttcttcgcta cctaaaatta atttgttatc taacatgatt
     3601 ttgattgatg atatataaaa aaagccacga attcagaatt aaaaatagtt ataattcatg
     3661 aattcgtggc ttaaaataaa cttactttta actcgttggt ttttctgctt ttttaacttt
     3721 cacatttaat tcttgtgcat catcctcaat atccataaaa atttcatctc catcaccaat
     3781 ttttgaagtg ataatttcct ctgctaaaga atcttcaaca tatttttgaa tggctctttt
     3841 taatgggcga gcaccaaatt gcttatcaaa acctttttca gcaataaatg ctttggcttt
     3901 atctgttaaa gtcaaggaat aacctaaatc ttttatacga gagtataatt tttttaattc
     3961 aatttcgata attaaattaa tatcttcttt ttctaaagca ttgaaaacaa tgacatcatc
     4021 aatacgattc aaaaattcgg gagcaaatgt tttcttcaag gcattttcaa cgacactttt
     4081 agaatgttca tccgcttggg aaacttttgc agcagttcca aatccaactc cttgtccaaa
     4141 atctttcaat tgacgtgctc caacattgga tgtcatgatg ataattgtat ttctaaaatc
     4201 gatttttcgg cctaaactat cggtcatgta tccatcatct aaaacctgaa gcatcatatt
     4261 aaacacatcc ggatgagctt tttcaatttc gtctaataaa actacacaat agggtttgcg
     4321 acgaa
//
LOCUS       DAOTCW010000174         4310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_15844_length_4310_cov_32.600940, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000174 DAOTCW010000000
VERSION     DAOTCW010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15844_length_4310_cov_32.600940"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..2007
                     /locus_tag="P5335_10775"
     CDS             46..2007
                     /locus_tag="P5335_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRZ75406.1"
                     /translation="MFFSAFKLPNRYKLLIGFSKWFLLFSFIIRIIFIFWKLDEVSSN
                     PIDLIITFGYGIFFDIGVLSFIWLFSTFYITLLPNNWIGSIADKVLIYTFFSITILVL
                     IFTFFAEITFWDEFKSRFNFVAVDYLIYTFEVVQNINQSYPLVILIPSMLFLAFLVFY
                     YFVKKEYFKSVFQDKIPFSTRVVPFVTVITINLIYVFFINNSDAEWSKNRYNNEISKA
                     GIYSFFAELRNNKLDFPTFYSTVSNEEAFTLVRSNLAEPNSKFITNDYSVKRKINDTL
                     DTNQKPNVIFILMESMSAEFMKEFGNDKAITPFMDELAQKSLLFTNLYATGTRTVRGM
                     EAVTLSIPPTPGSSIVKRPDNAGLFTISNVFKERNYQCNFFYGGDGYFDNMNAFYGGN
                     GFDIYDRGRGSVLSDDIKTTRHNINDNEVTFENAWGICDEDIYNVVIKVADEQHAKGK
                     PFFNFVMTTSNHKPYTYPDKKIVIPSGTGRNGAVQYSDYAIKHLFEKAKDKPWFKNTI
                     FVLVADHCASSAGKDEIDVKNYHIPAIIFGAGVPVQKVDKLCSQIDLFPTLFGVLNWN
                     YTSDFFGKNVLAPTFEERTFMGTYLKLAMMKKDNSVMILSNQKKEHQYTWNLKDNSLK
                     NVKLNPNFKEETTAFYQSADYLFSNHLLN"
     gene            2129..2563
                     /locus_tag="P5335_10780"
     CDS             2129..2563
                     /locus_tag="P5335_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013304955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRZ75407.1"
                     /translation="MKRIFVVVVFFLSFSAIAQNWQTSFEEAKSKAIKENKNIVLVFS
                     GSDWCAPCMKLEKNVWKSTAFQTEAEKNWVIYKADFPKKKANQLNPELTEQNKKLAEK
                     YNQNGSFPLVLLLDKNGKALGMTGFKNVSAEEYVNLIKSFQK"
     gene            2649..3638
                     /locus_tag="P5335_10785"
     CDS             2649..3638
                     /locus_tag="P5335_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRZ75408.1"
                     /translation="MKHFSMVFIFFNVILMQGQTVVKRKTMMLGSPFEMTVVANDSLQ
                     GNVYIDQAVAEVKRIENLISDWIPTTQISIVNQKAGIEPVKVDREVFELVERAIKISQ
                     LTSGAFDISYASMDKIWKFDGSMKEMPTEEAIKNSVSKVGYQNIILNEKESTLFLKNK
                     GMKIGLGGIGQGFIADKIKALLVQNGCQSGIVNVSGDIFAWGKQPDGNPWTVAIVNPM
                     NKNKVFATFPLEDSAVETSGSYEKFVTFNGKRYSHIIDPRTGYPASGIVSVSVFAKTT
                     ELADALATGIFVLGVEVGLDLVNQIKGVECIIVDDKGGVHTSNNIDLKKLNKN"
     gene            3701..3910
                     /locus_tag="P5335_10790"
     CDS             3701..3910
                     /locus_tag="P5335_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HRZ75409.1"
                     /translation="MKKFSILVLILFALQSCSSVKEYEKEKINDPDMKLAARSTERFE
                     TNFQVYREAAAGANGGKTGGGCGCN"
     gene            3932..>4310
                     /locus_tag="P5335_10795"
     CDS             3932..>4310
                     /locus_tag="P5335_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75410.1"
                     /translation="MKNYLIAIFSFLSFSLFAQEKDSTLIFKKRVLEAVEVDFLMSYY
                     NQDGIHSAVSGGKGMEELTDITPTIVVAIPMNEDDVLTVDAGISAYSSASSGNINPFD
                     SATPSPWQASSGASAQDVLSTISL"
BASE COUNT         1467 a          614 c          748 g         1481 t
ORIGIN      
        1 gcaagtataa aacagtacgt tggatatttg ttgaaaattt gtattatgtt tttttcagca
       61 tttaaacttc ccaatcgtta caaattattg attggatttt cgaaatggtt cttactcttt
      121 tcctttataa ttagaatcat ttttattttt tggaaacttg atgaagtttc atcaaatcca
      181 attgatctga ttataacctt tggatatgga attttctttg atatcggagt actgtcattt
      241 atttggctct tttcaacttt ttatatcaca cttttaccaa ataattggat tggttcaata
      301 gctgataaag tattaattta cacattcttt tcaattacaa tacttgtttt aatattcacc
      361 ttttttgccg aaatcacgtt ttgggacgaa ttcaaaagtc gttttaattt tgtagctgtc
      421 gactatttaa tttacacttt tgaagtcgtt caaaacatta atcaaagtta tccgttggtt
      481 attttaattc catcaatgtt gtttctcgct tttttagtgt tttattattt tgtaaaaaaa
      541 gaatacttca aaagtgtctt tcaagataaa attccatttt caacaagagt tgtcccgttt
      601 gtaaccgtga ttacaatcaa tttaatatat gtttttttta ttaataattc tgatgcagaa
      661 tggtcaaaaa accgatacaa taatgaaatt tctaaagcgg gtatttattc cttttttgct
      721 gaattaagaa ataataaatt ggatttccct actttttatt caaccgtttc aaatgaagaa
      781 gcatttactt tagtgagaag taatttagca gaacctaatt caaaatttat cacaaatgat
      841 tattctgtta aaagaaaaat aaatgatacg ctggatacaa atcaaaaacc taatgtgatt
      901 tttattttga tggaaagtat gagtgctgaa tttatgaaag aatttgggaa tgataaagca
      961 ataactcctt ttatggatga attagcccaa aaaagtttac tatttacaaa tttatatgcc
     1021 accggaacgc gaacagttag aggaatggag gcggtaactt tgtctatccc accaactccg
     1081 ggaagcagta tcgtaaaacg acctgataat gccggacttt ttacaatttc aaatgtattc
     1141 aaagaacgca attatcaatg taattttttc tacggtggcg atggttattt tgacaacatg
     1201 aacgcatttt atggcggaaa tggttttgat atttacgata gaggtagagg cagcgttcta
     1261 agcgacgata ttaaaacaac tcgtcataac attaatgata atgaagttac ttttgaaaat
     1321 gcatggggaa tttgtgatga ggatatttat aatgtagtaa ttaaagtggc cgatgaacaa
     1381 catgccaaag gaaaaccgtt tttcaatttt gtgatgacaa catcaaatca taaaccatac
     1441 acttatcctg ataaaaaaat agttattcct tcgggaacag gaagaaatgg agcggttcaa
     1501 tattctgatt atgctattaa acatttgttt gaaaaagcaa aagataaacc ttggtttaaa
     1561 aacaccattt ttgttttagt ggcggatcat tgtgccagtt ctgccggaaa agatgaaatt
     1621 gatgtgaaaa attaccacat tccggcaatt atatttgggg caggagttcc ggtacaaaaa
     1681 gtagataaat tgtgttctca gatagatttg tttccaacac tttttggtgt tttaaattgg
     1741 aattatactt cagatttttt tggtaaaaat gttttggcac ctacttttga agaacgaaca
     1801 ttcatgggaa cttatttgaa actagccatg atgaaaaaag acaattcggt tatgatttta
     1861 tccaatcaaa aaaaagaaca tcaatatact tggaatttaa aagataattc acttaaaaat
     1921 gttaaactaa atcccaattt taaagaagaa accactgctt tttatcaatc tgcagattat
     1981 ttgttttcaa atcatctttt gaattaaaat taagatttac ttaagtatgt atacacattg
     2041 caacatctaa aataaaacaa cgttattttg tataatattt taaataataa aacatttcat
     2101 tttatacctt tatagtaatt aaatttttat gaaaagaatt ttcgttgtag tagtcttttt
     2161 tttgtcattt agtgctatag cacaaaattg gcaaactagt tttgaggaag ctaaatccaa
     2221 agccattaaa gaaaataaaa atatagtatt ggttttttcc ggttctgatt ggtgtgctcc
     2281 ttgtatgaaa ttagaaaaaa atgtttggaa atctactgct ttccaaactg aggctgaaaa
     2341 aaattgggta atttacaagg ctgattttcc taagaaaaaa gcaaatcaat taaatcctga
     2401 gcttactgaa caaaacaaaa aattagctga aaaatataat caaaacggaa gttttccatt
     2461 agttctttta ttggataaaa atggaaaagc gttaggaatg acaggcttta aaaatgtttc
     2521 agcggaagaa tatgtcaatt taattaagtc gtttcaaaaa taatgactgt tttcaataga
     2581 tgttttacgg ataatttatt tttatgttgt tttgattatg atttgtttgt caataaaatt
     2641 ctaatagcat gaaacatttt tcaatggtat ttattttttt taacgtcatt ttgatgcaag
     2701 ggcaaactgt cgtcaaaaga aaaacaatga tgctcggtag tccgtttgaa atgactgttg
     2761 tggcaaatga ttccttgcaa ggaaatgttt atatcgacca agctgttgct gaagttaaac
     2821 ggattgaaaa tttaatttca gattggattc caaccacaca aatttctata gtcaatcaaa
     2881 aggcaggaat tgaacccgta aaagttgata gagaagtttt tgaattagtt gaacgtgcaa
     2941 ttaaaatctc tcaattaaca agtggtgctt ttgatatttc ctatgcttct atggataaaa
     3001 tttggaaatt tgatggcagc atgaaggaaa tgccaacaga ggaagcaatt aaaaattctg
     3061 tgtctaaagt tggttatcaa aacatcatct taaacgaaaa agaatctact ctttttctaa
     3121 agaataaagg gatgaaaatt ggtttaggag gaatagggca agggtttatt gcggataaga
     3181 ttaaagcact cttggttcaa aacggatgtc aatcgggaat agtcaatgtt tctggggata
     3241 tttttgcttg gggaaaacaa cccgatggta atccttggac tgtagctatt gttaacccca
     3301 tgaataaaaa taaagtattt gccacttttc cattagaaga cagtgctgtt gaaacttcag
     3361 ggagttatga aaaatttgtc acttttaacg ggaaacggta ttcacacatt atagatccaa
     3421 gaacaggtta tcctgcttcc ggtattgtca gtgtatctgt atttgcaaaa accacagaac
     3481 tcgcagatgc actggccact ggaatttttg tattaggggt agaagttggg ctcgatttag
     3541 tgaatcaaat taaaggtgtt gaatgtatta ttgtagacga taaaggcgga gtgcatactt
     3601 ctaacaatat tgacttaaaa aaattaaata aaaattaatt atttcactcg ctcctctctc
     3661 ctttggagag gggctggggg tgaggttaaa taatcaaatc atgaaaaaat tctcaatctt
     3721 agtattaatt cttttcgctc tacaatcttg cagttcagtt aaagaatacg aaaaggaaaa
     3781 aataaacgac cctgacatga aattagccgc tcgttcaaca gaacgttttg aaactaattt
     3841 tcaagtgtat cgggaagctg ctgcgggagc aaatggtggt aaaaccggtg gtggttgtgg
     3901 ttgtaattaa taagttgaat tcctttttat catgaaaaat tacctaatag caatattttc
     3961 ctttctttcc ttttcccttt ttgcccaaga aaaagattct acacttattt tcaaaaaaag
     4021 agtgttagaa gctgtagaag ttgacttttt aatgagttac tataatcaag atggaattca
     4081 ttctgctgtt tccggaggaa aaggaatgga agaacttacc gacattacac caactatcgt
     4141 tgtagctatc ccaatgaatg aagatgatgt tttaacagtt gatgccggta tttccgccta
     4201 ttcttctgct tcatcaggaa atattaaccc gtttgacagt gctacgccca gtccctggca
     4261 agctagttca ggtgcttctg ctcaagacgt attgtcaaca atttctctta
//
LOCUS       DAOTCW010000175         4298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_15911_length_4298_cov_53.979260, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000175 DAOTCW010000000
VERSION     DAOTCW010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15911_length_4298_cov_53.979260"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..229)
                     /locus_tag="P5335_10800"
     CDS             complement(2..229)
                     /locus_tag="P5335_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRZ75411.1"
                     /translation="MIPEPKKKPLNKWLQLINIPIQMGVVIFIFSYFGQFLDEKYGNV
                     NESNTKIFTLLGVFLALYNVYRQVNQLSKEE"
     gene            complement(198..605)
                     /locus_tag="P5335_10805"
     CDS             complement(198..605)
                     /locus_tag="P5335_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRZ75412.1"
                     /translation="MFKKDSKSYTDLLGKTNRIVESTIIKGDISSSADFRLDGHLVGN
                     FQSKGKLVIGPAGSVKGDIICKNADIEGKFDGKIQVTEILSIKSKASIHGEVICGKLA
                     VEPGAEFSATCVMKPNVKSLLPQNDSGTEEKTA"
     gene            complement(614..3454)
                     /locus_tag="P5335_10810"
     CDS             complement(614..3454)
                     /locus_tag="P5335_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HRZ75413.1"
                     /translation="MPLNVFILKSSIFRYIFASFLLVFLIACSTRKDKLVNRKFQALN
                     TEFNVLYNGNLALEAGLKDIVTNYNDNYWEILPIERMQVAEEEILPGENKNANFERAE
                     DKATKAIQKRSMLIEGSEKNPQMDEAYLLLGKSRYYDQRFIPALEAFNYILYKNPDSD
                     KIYEAKIWREKTNVRLENDALAVNNLTKLLNEIKFKDQIFADANATLSQAYLNLEQKD
                     SAISKLKQALNFTKKDEEKARYRFILGQLFESINEKDSAFAAYQSVIDMKRKSPRQYI
                     VHAQIKQAQQFDFTKGDTLAFMTEYNKLIDDRENRPYLDFLFHQKALFYHQQDNAKEA
                     VFNYNNSLDYQTSDKYMMASNYRNIAEIYFYKAEYQTSGKYYDSTLVLLNQRTREFKA
                     IRKKRDNLEDVIKYEGIAQRNDSILNIASLSEADKIAFYEDYINKIKKEDEFTAEAAK
                     KLREVSEDDSKSGNSQNQNQLGIKNKASLGQASPKSSTFYFYNQSSIDFGKKEFIKNW
                     GKRSSEGNWRLSNKKQTISTEEEEEVVENTKEEKIVDQKDNLRYSPEFYIGQLPSSKK
                     ELDSIAKERNYAYYQLGVIYKEKFKEYQLAADRLEILLKSKPEERLVLPALYNLYKIY
                     EIIDPAKAVTMKENIITQFPDSRYAQILSKSGTSAENLIDDPEIVYKNLFKVYEDRDY
                     RQVIVSVEPAIEKFTGEETLPKFELLKANVIGKIKGIFEYRKALNYVSLTYPNSEEGK
                     EAELLLSDNIPYLESLYFDQAMPTSYKVLYHADNLEDKKTKVLIEKVAKFIKERNQSQ
                     LTTSIDLYTMDKNFFVIHGLRDEEYAKGVVSILKEFKDYKISEKAIIISSENYKIVQI
                     RKNLEEYLNPDLRVVPPPYVAKPKQPKQTEMKQEIVIPNKNQKKPTQTQSTNNMQPPS
                     GIDDPKSTPKGNTRQDPESQMMPPSPNNPKKG"
     gene            complement(3597..4292)
                     /locus_tag="P5335_10815"
     CDS             complement(3597..4292)
                     /locus_tag="P5335_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRZ75414.1"
                     /translation="MIQVQNLTKTYNGTTVLNIEHLEIPKGQSFGLVGNNGAGKTTFF
                     SLLLDLIQPTTGFITSNSIQINTNEKWKPFTSAFIDESFLIGYLTPEEYFYFIGELRG
                     WNKADVDSFLVKYEDFFNGEILKNKKYLRDLSKGNSKKVGIVAALIGEPEVIILDEPF
                     ANLDPTTQIRLKKIIKELADDNSVTILVSSHDLQHTVEVSNRIVALQKGEIVLDLIPS
                     SETLKELEAFFAA"
BASE COUNT         1263 a          732 c          640 g         1663 t
ORIGIN      
        1 atcattcttc tttgcttagt tggttcactt gacgatagac attatacaaa gctaaaaaaa
       61 caccaagtaa agtaaaaatt tttgtgtttg attcgtttac attgccatac ttttcatcta
      121 aaaactgtcc gaaatacgaa aaaataaaaa tcaccacgcc catttggatg ggaatattga
      181 tgagttgcag ccacttatta agcggttttt tcttcggttc cggaatcatt ttgaggtagt
      241 agtgatttta cattaggttt catcacgcag gtagcactaa actcagcacc gggttctacg
      301 gccaattttc cgcaaataac ctcaccgtgg atacttgctt ttgatttgat acttaaaatt
      361 tctgtaacct gtatttttcc gtcaaactta ccttcaatat cggcattttt acaaataata
      421 tcgcctttaa cgcttccggc ggggccaata actaattttc cttttgattg aaaattgcca
      481 actaaatgac catctaatct aaaatcagca gaggaagaaa tatctccttt aataatggta
      541 ctttcaacta ttcggtttgt tttcccaagt aaatcggtat atgattttga atctttttta
      601 aacatgctca tggttaccct ttttttggat tattaggaga aggcggcatc atttgtgatt
      661 caggatcttg ccttgtattt ccttttggag tagattttgg atcgtctata cctgaaggag
      721 gctgcatatt attagtgctt tgagtttgtg ttggcttttt ctggttttta tttggaataa
      781 caatttcctg tttcatctct gtttgttttg gttgttttgg cttggcaacg tatggcggcg
      841 gaacaactct taaatcaggg ttcaaatatt cttctaaatt ttttctgatt tgaacaattt
      901 tataattttc agaggaaatt ataatcgctt tttctgaaat tttataatct ttaaactctt
      961 tcaagattga cacaacacct ttagcatact cttcgtctcg caaaccatga attacaaaga
     1021 aatttttgtc catagtatac aaatctattg aggtagttaa ctgactttgg tttctttctt
     1081 taatgaattt agctactttt tcaattaaaa ccttcgtctt tttatcctct aaattatctg
     1141 catgataaag cactttataa ctggttggca tagcctgatc aaaatacagg ctttctaaat
     1201 aagggatatt atctgaaagc agtaattcag cttcttttcc ttcttcagaa tttggataag
     1261 ttaaagacac ataatttaaa gcttttctgt attcaaaaat ccccttaatt ttccctatta
     1321 catttgcttt aagcaattca aatttaggca aggtttcttc tcctgtgaat ttctcaatag
     1381 ccggttctac gcttacaata acttgtctgt aatctctatc ttcatatact ttaaacaagt
     1441 tcttataaac aatctccggg tcatcaatta gattttcagc tgatgttccc gatttactta
     1501 aaatttgagc atacctggaa tccgggaatt gagtgatgat gttctctttc atcgtcactg
     1561 cttttgcagg atcaatgatt tcgtatattt tatataaatt atacaatgcc ggcaacacca
     1621 atcgttcttc aggtttgctt ttcaataaaa tttctaatcg atcagcagct aattgatatt
     1681 ctttaaattt ttctttatag attactccca attgataata ggcatagttt ctttctttcg
     1741 cgatgctatc taattccttt tttgaactag gcaattgtcc aatataaaat tcaggggaat
     1801 acctcaagtt atctttttgg tcaacaattt tttcttcttt tgtgttttca acaacttctt
     1861 cttcctcttc tgttgaaata gtttgttttt tgttggaaag tctccaattt ccttctgatg
     1921 atcttttccc ccagttttta ataaattctt tttttccaaa atcaattgaa ctttggttat
     1981 aaaaataaaa agtggatgat ttaggacttg cttgtcctaa actggcttta tttttaattc
     2041 cgagttgatt ttggttttga gaatttccgg attttgaatc atcttcactt acttctctga
     2101 gttttttagc tgcttctgca gtaaactcat cctctttttt tattttattg atgtaatctt
     2161 cataaaaagc aattttatca gcttctgata atgaggcaat atttaaaatg ctgtcatttc
     2221 gttgagcgat tccttcatat ttaattacat cttctaaatt atctctcttt ttgcggattg
     2281 ctttgaactc tcttgttctt tgattaagca ataccaaagt actgtcataa tattttccgg
     2341 aagtttgata ttcagctttg taaaaatata tttcagcaat atttcgatag tttgaagcca
     2401 tcatatattt atcacttgtt tgataatcca aagaattgtt ataattaaaa actgcttctt
     2461 tggcattatc ttgttggtga taaaacaacg ctttttggtg gaataaaaaa tctaaataag
     2521 gtctgttttc tcggtcatca atcaatttat tatactctgt catgaaagct aacgtgtctc
     2581 ctttagtgaa atcaaactgt tgagcctgtt taatttgagc atgaacaata tattgtcttg
     2641 gagattttcg cttcatatcg ataacggact gataggcggc gaaagcactg tctttttcgt
     2701 ttatagattc aaataattgt cctaaaataa agcgataacg agctttttct tcgtcttttt
     2761 tagtaaaatt taaggcttgt tttaatttag aaatggcact gtctttttgt tctaaattaa
     2821 gataggcttg cgacaatgtg gcatttgcat ctgcaaaaat ctggtcttta aatttaattt
     2881 cgtttaataa ttttgtaagg ttattaacgg ctaaagcatc attttccaaa cgaacattgg
     2941 ttttttcacg ccaaatctta gcttcataaa ttttatcact atccggattt ttatataaaa
     3001 tataattgaa tgcttctaat gctgggataa aacgctggtc gtaatatcgt gattttccca
     3061 agagcaaata tgcttcatcc atttgagggt ttttttcgct tccctcaata agcattgacc
     3121 gtttttgaat tgcttttgtt gctttatctt cggctctttc gaaatttgca tttttatttt
     3181 cccccggaag aatttcttcc tctgcaactt gcattctttc aattggaagt atttcccaat
     3241 aattatcatt ataattggtt acaatatctt ttaacccagc ctctaaagct aaatttccat
     3301 tatataatac attaaattct gtattaagtg cctgaaattt tcgattcact aatttatctt
     3361 ttctggtaga acaagctatc agaaaaacta ataaaaaaga agcaaaaata tatctaaaga
     3421 tagaggattt caaaataaaa acgtttaatg gcataactaa aaagtactgt ttttagtata
     3481 tcgcaagggt aaaaatacgt ttctttttga aatagagaaa aatattgcgg tttttttgtt
     3541 cagaaacgtt cttactgact aaattaaaag aaagaacgtt cctgtttttt gtccatttaa
     3601 gcagcaaaaa aagcttctaa ttctttcagc gtttcagaag aaggaattaa atctaaaact
     3661 atttctcctt tttgtaaagc cacaattcgg ttggaaacct ctacagtatg ttgcaaatca
     3721 tgactggaaa ccaaaatagt cacactgttg tcatctgcta attctttaat gatttttttg
     3781 agtcgaattt gtgtggttgg gtctaaatta gcaaatggtt catctaaaat aattacttcc
     3841 ggttctccta ttaatgcggc aacaataccc acttttttag aatttccttt tgataaatcg
     3901 cgtaaatatt tcttgttttt taaaatttct ccattaaaaa aatcttcata ttttactaaa
     3961 aaactatcca catcagcttt gttccaaccg cgaagttcac caataaaata gaaatattct
     4021 tccggagtta aataaccaat taagaaactt tcgtcgataa aggctgacgt aaaaggtttc
     4081 catttttcat tcgtgttaat ttgaatagaa ttactggtaa taaaacctgt tgttggctga
     4141 attaaatcta acaacaaact gaaaaaagtt gttttccctg ctccgttatt tccaaccaac
     4201 ccgaaacttt gcccttttgg aatttcgagg tgctcaatat ttaaaacggt ggttccgtta
     4261 tatgttttgg taaggttttg aacttgtatc atggtttt
//
LOCUS       DAOTCW010000176         4254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_16174_length_4254_cov_33.166468, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000176 DAOTCW010000000
VERSION     DAOTCW010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16174_length_4254_cov_33.166468"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="P5335_10820"
     CDS             complement(<1..479)
                     /locus_tag="P5335_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRZ75415.1"
                     /translation="MTTAILIDDDANLRAGMRQLLSIYAPEINVLGEAESVQSGVEAI
                     NLLQPQVIFLDIQLSDGTGFDLLEKLAEMNGKISSQVVFITAHEQYAIKAFRFSALDF
                     LLKPVDPEELQKVIYKIKNVLAKSDNYAHIDLLLENIRRKVDNFKRIALSTSDGIHL"
     gene            complement(525..>1145)
                     /locus_tag="P5335_10825"
     CDS             complement(525..>1145)
                     /locus_tag="P5335_10825"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRZ75416.1"
                     /translation="LHLQMNPHFVFNCLGSISSFIVQNGTDSAIKYLSKFSKLMRLTL
                     EYSKESLIPIDKEIESLQNYLEFEQLRFNNVFDFKITKSKEIEDDMALPPLLLQPFVE
                     NAIIHGLIPKKENGIISVDFSIEKESLICTIVDNGIGFEKSKEIKENLVAIHKSMALD
                     ITKKRLEMMEASTAQKAKVEIKEIKNDSGEIQGTKVVLNLPIQYIK"
     assembly_gap    1146..1245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1246..2470)
                     /locus_tag="P5335_10830"
     CDS             complement(<1246..2470)
                     /locus_tag="P5335_10830"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRZ75417.1"
                     /translation="MENYARQALQLSQKIKYKAEEGNALLNLGIAQIISGNYPKAIDF
                     FSQAKLIFENELAIGSKEELKVKSGLARTYGSMGIVFSEQSNYAKALQYHIKAVAIYE
                     EMKDEEKTARVYNNIGIVYRSQKEDFKALDYFSRAQKLQKRIGDGTVGITTTNIGNLY
                     LQQKNYEKANQYYLEAKQLFEKYPNDRGQGELFNNIGLYFKETNNQPKAFESWELALQ
                     SFAKIDDRFGTSDTYLLMGECYYDQKRFTEALEYAQKAAKLSKDLNVLEGTALAEKLL
                     SSIYEKLNNSTAAFTHFKLYKIAQDSLINHENIRKIVQTEMNYEFEKKEALQKKEQEK
                     KEVLLAEKSKRQTLKTIFIALFVLLLMGIAFLIYNRMQLKKTLTLQKELAEYEQKALH
                     LQMNPHFVFNCLGSIS"
     gene            2801..3328
                     /locus_tag="P5335_10835"
     CDS             2801..3328
                     /locus_tag="P5335_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRZ75418.1"
                     /translation="MERKVKYDYAFKIECIKLIIEQGHSCESVSVQKGVHESIIRKWL
                     RFYNAYGLDGLLPRKNQDYSIDFKLKVLKSIAEQHLSLHQAYVKFNIPTRSIIAKWQR
                     DFTNFGVEGLTQKPKGRPKSMDNFKRKKRKSDKPLTREEELLQEIERLRCENALLKKL
                     QALIQAEEEAKRHKL"
     gene            3328..3840
                     /locus_tag="P5335_10840"
     CDS             3328..3840
                     /locus_tag="P5335_10840"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRZ75419.1"
                     /translation="MELRHDFDLNLLLNQTNIARSSFYYHQKKGKLPDKYKKVKELIK
                     SIYHKHKGRYGYRRITDELNNKGIIINHKTVLRLMKSLSLKSIIRIKKYNSYKGEQGK
                     IAPNILDRNFKATAPNQKWATDITEFNVSGKKLYLSPIIDLFNQEIISYELTERPMFN
                     QVAMMLKKSF"
     gene            3845..4162
                     /locus_tag="P5335_10845"
     CDS             3845..4162
                     /locus_tag="P5335_10845"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRZ75420.1"
                     /translation="MPNNTNLTLHSDQGWQYQMRQYQYLLKQKGITQSMSRKGNCLDN
                     AIIENFFGILKSELFYIQKFASLEELKHEIKQYINYYNKERIKSNLNKMSPIQYRAHY
                     YQN"
BASE COUNT         1438 a          669 c          600 g         1447 t
ORIGIN      
        1 aacaaatgaa ttccgtctga agtggaaaga gcaatccgtt tgaaattatc cactttccgt
       61 cttatatttt ctaacaacaa atcgatgtgg gcataattgt cgctttttgc taatacattt
      121 ttaattttat aaatcacttt ttgcaactct tccgggtcta ccggtttcaa caaaaaatct
      181 aatgcactaa atcgaaatgc cttaatggca tattgttcat gagctgttat aaaaacaact
      241 tgtgaagaaa tttttccatt catttctgct aatttttcca acaagtcaaa accggtacca
      301 tcgctaagtt gaatatctaa gaaaattact tgtggttgta acaaatttat tgcttcaaca
      361 ccggattgca cactctctgc ctcgccgaga acattaattt ccggagcata tatggaaagc
      421 aattgtcgca ttcctgctct taggttggca tcatcgtcta ttaatattgc tgttgtcatg
      481 tattaattgc gggtttcaag tttcaggttg aatcaacaag ttatttactt tatatattgt
      541 attggtaaat tcagaactac ttttgtccct tgaatttcac ctgaatcatt tttaatttct
      601 ttgatttcaa cttttgcttt ttgtgctgtt gaagcctcca tcatctcaag tctttttttg
      661 gtgatatcta acgccatcga tttgtgaata gccactaaat tttctttgat ttcttttgat
      721 ttctcaaaac ctattccgtt gtcaactata gtgcaaataa gactttcctt ttcaattgaa
      781 aaatctactg aaattattcc gttttctttt ttgggaatta acccgtgaat gattgcattc
      841 tctacaaaag gttgcaacaa caaaggaggc aaagccatat cgtcctctat ctctttgctt
      901 ttggtaattt taaaatcaaa cacattattg aaacgcaatt gttcgaattc caaataattt
      961 tgcagacttt caatttcttt gtcaatggga attagtgatt ctttcgaata ttctaatgtt
     1021 aatcgcatta attttgaaaa ttttgacaaa tatttaatcg cagaatccgt tccgttttga
     1081 acaataaaac tggaaataga acctaaacaa ttgaaaacaa aatgcggatt catttgaaga
     1141 tgcaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntggaa atagaaccta
     1261 aacaattgaa aacaaaatgc ggattcattt gaagatgcaa cgctttttgt tcatattctg
     1321 ccaattcttt ctgtaaggtc aatgtctttt tgagctgcat tcggttataa atcaaaaagg
     1381 caattcccat tagtaacaag acaaataatg caataaaaat ggtttttaaa gtttgtcttt
     1441 ttgatttttc agctaataaa acttctttct tttcctgttc ttttttctgc aaagcttcct
     1501 ttttttcaaa ctcataattc atttcagtct gcacaatttt cctaatattt tcgtggttta
     1561 tcaagctgtc ttgtgcaatt ttatacaatt taaaatgagt gaaagctgct gtagaattat
     1621 ttaatttttc ataaatcgaa ctcaataatt tctcggctaa agctgttcct tccaacacat
     1681 ttaaatcttt agacaatttt gctgcttttt gagcatattc caatgcttct gtaaatcttt
     1741 tttgatcata ataacattcc cccatcaaaa gataagtatc cgatgttcca aatctatcat
     1801 caattttagc aaacgattgt aatgccaatt cccaactttc aaaagctttt ggctgattat
     1861 tagtttcttt aaaatacaac ccaatattat taaataattc accttgacct ctatcattag
     1921 gatatttttc gaagagctgt tttgcttcta aataatattg atttgctttt tcataattct
     1981 tttgctgtaa ataaagattt cctatgttgg ttgtagttat accaacggtt ccatctccaa
     2041 ttcttttctg taatttctgt gctctactaa aataatccaa tgctttaaag tcttcctttt
     2101 gacttcgata aactattccg atattattat acactcgagc agttttttct tcatccttca
     2161 tttcttcata aatagcaaca gctttaatgt gatattgcaa agctttggca taattacttt
     2221 gctcagagaa aacaattccc atactaccat aggttcgagc taaaccgctt ttaaccttca
     2281 attcttcttt agaaccaata gctaattcat tttcaaaaat taatttagct tgtgaaaaaa
     2341 aatcaattgc tttaggataa tttcctgaaa ttatttgggc aattccaaga ttaagtaaag
     2401 catttccttc ttcagctttg tattttattt tttgagaaag ttgtaatgcc tgcctagcgt
     2461 aattttccat taattttgga ttactacttt tataatcatc cgcgattgca ttaagaaatt
     2521 gcgttttttc tatttcattt tgagtttggt taaggagatt gaccaaacta tcaacccttt
     2581 tattttgccc aaataaattt gtagcaagga agagacaaaa gaaaaataat tgtagaaact
     2641 tcttcataaa aatgaattga tgaacaaata taagttttca tctttgaagt tgcacactaa
     2701 atgagtaatt gcaggttttg agtgagtatt tgagtaaaaa caaaaaccct ctcagtatac
     2761 actgccccca aaaagttaga cactatttgg gggtattttt atggaaagaa aagtcaagta
     2821 tgattatgca ttcaagatag aatgtataaa attaattatc gaacagggtc attcctgtga
     2881 atcagtttca gtgcaaaaag gcgttcatga atcgattatt cgtaagtggt taaggtttta
     2941 caatgcctat ggtttggatg gtttattgcc aagaaaaaat caggattatt ctattgattt
     3001 taaattaaaa gttttaaaaa gtattgcgga gcaacactta tcattgcatc aagcttatgt
     3061 taagtttaat attccgacac gaagtataat tgcaaaatgg caaagagatt ttactaactt
     3121 tggggttgaa ggtttaactc aaaaaccaaa aggcagacca aaatctatgg ataacttcaa
     3181 acgaaaaaaa cgtaaatcag acaaacctct aacccgagaa gaagaactat tgcaggaaat
     3241 agaaagactt cgttgtgaaa atgctttgct aaaaaagtta caagccttaa ttcaagccga
     3301 agaagaagcc aaaaggcaca agctataata gaattaaggc atgattttga tttaaatctc
     3361 cttttaaatc agaccaatat agcacgaagt agtttttatt accatcaaaa gaaaggtaaa
     3421 ttacctgata aatataagaa agttaaagaa ctgattaaat ctatttatca caagcacaaa
     3481 gggcgttatg gttatagaag aatcactgac gaacttaata ataaaggaat aattatcaat
     3541 cataaaacgg ttttaagatt aatgaaatca ttaagtttaa agagcatcat aagaatcaaa
     3601 aaatacaatt cttataaagg agaacagggc aaaattgctc caaatatttt ggaccgcaat
     3661 tttaaagcaa cagcaccaaa tcaaaaatgg gctactgaca taactgaatt taatgtgtct
     3721 ggaaagaaat tatatttatc accaataatt gacttgttta accaagaaat tataagttat
     3781 gaactaacgg aaagaccaat gtttaatcaa gtggcgatga tgcttaaaaa aagcttttaa
     3841 gaaaatacca aataatacca atctcacttt acactctgat caaggctggc aatatcaaat
     3901 gagacaatat caatatttat taaagcagaa aggaattact caaagtatgt caagaaaagg
     3961 aaattgtttg gataatgcta taattgaaaa cttctttgga atactaaaat cagaactctt
     4021 ttacatacag aaatttgcaa gtttagaaga actaaaacat gaaatcaaac aatacatcaa
     4081 ctattataat aaagaaagaa ttaaatcaaa tttaaacaaa atgagcccaa tacaatatcg
     4141 agctcattat tatcaaaatt agatataaat ttgtctaaac ttttgggttc agtctagtat
     4201 tgaaagggtt tttagttaat tccattattc tttaataatt ttatgtgttt tggt
//
LOCUS       DAOTCW010000177         4198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_16485_length_4198_cov_47.904176, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000177 DAOTCW010000000
VERSION     DAOTCW010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16485_length_4198_cov_47.904176"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1600)
                     /locus_tag="P5335_10850"
     CDS             complement(137..1600)
                     /locus_tag="P5335_10850"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HRZ75421.1"
                     /translation="MQAQTDKNTSREEQKTKAASIPDKIQTVAGELQFFDGVPIGNTN
                     DLVYDYLNRARAVDVYLDNVGAVSIYAVLSGMAEQGADAPNKIAIWEQLMDSRTPVIT
                     SNTSTMYAYGSTDLAKDGPTVIEIPPGMLGFLDDAWQRYVGDMGITGPDKGKGGKYLL
                     LPPGYTGDVPTGYFILKPRTNKNFLFLRGSIKDGLEAAVDNFKKGLKIYPLKDKDNPA
                     PTELINMSGRSFSTIFPSDLKYYEILNAIIQDEPLDAINSEVRGEIASIGIVKGKPFN
                     PDANMKKLLTEAAILGNAAGRSITYDPRIAGVYIYPDSKSNWVMGYANKNTSFEADNT
                     MNLDARVLFYYNAGGVTPAMAVTHAGAGSDYALGMLDGNDKPFDGAKTYKLHIPTNVP
                     VNNFWAVTLYDTQTRCQLQTDQIFPTVGSQDKEIKKNADGSYDLYFGPKAPKGKEGNW
                     LQTIPGKSWFSILRMYGPLEPWINKTWRPGEIKLVNE"
     gene            complement(1738..2544)
                     /locus_tag="P5335_10855"
     CDS             complement(1738..2544)
                     /locus_tag="P5335_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="neuromedin U"
                     /protein_id="HRZ75422.1"
                     /translation="MKKRNLLIIAFLISSGFAIGQEKEQEPAAGGDLQSKIQNPIASL
                     ISIPFQNNTDFGIGEDDRTRNTLNIQPVIPLSLNENLNLITRTIIPIISQPTFPGESE
                     FGLGDVSLSLFLTPAKPGKLIYGGGLALGLPTATDQYLGAEKWTAGPSVVLLVQPKGW
                     TVGTLIQNSWSYAGADDRGDVNFFYSQVFVTKNLQKGWYVNSAPIITANWEAPSGEQW
                     TIPLGAGIGKLIRLGKLPINAQAGYYVNVEKPTGGPDSQLRVQATLLFPK"
     gene            complement(2582..4063)
                     /locus_tag="P5335_10860"
     CDS             complement(2582..4063)
                     /locus_tag="P5335_10860"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HRZ75423.1"
                     /translation="MKKNKLKIIAITLIGVFTLITSCTQTEKKTAEATPDLEAIAERA
                     YLYGLQQAIYYGQRWTYTQNDSKENIVYSGLNQFAWVRKQITPDYPVVTPNATTLYGA
                     GFMDLREGPVVVEVPEITDRYYSFQVQDQYGIFRLIVGSPFNGTQARKYILVPPDFTG
                     NIPADFPTTDIIKWPFKTAFGLARMALMTSTDAEIKKINGYQDQLTMTPLKDWLANGH
                     KGIPQADRKIVKGDFPIPNGLPEYQFGQVDKQTAEQYYTLLHVILNDPTMPLIEDSKM
                     EAEMVEQLKVFNIGKGLSFEWNKLSPEQQTALKNGFKAGFDNVRKTLKTNMINMNGWG
                     VVRNDGGFETKWMDRAIIADAAWGAPDKNVSHGGAFLFTDADGKPFSGKNKYTLTFDM
                     NDLPPVTQFWSIPIYDSNGYFVANEINRYTVNSFMLAAGDIVPKDGKLVIYIQHEKPS
                     DPEQAKNWLPAPAEGFRFTSRFYGPYAPLTNGTYNMPKVFKVN"
BASE COUNT         1136 a          832 c          809 g         1421 t
ORIGIN      
        1 aacggtaaat tgtatgagta gtggcagatt gcgggctact tctctgtcaa accgctatgc
       61 agtttgtgcg ggcaataaac cttgctattt gtcagatctc ctgccattac ttatacaaaa
      121 tgttacagca cgttatttat tcatttacca actttatctc acctggtctc catgttttat
      181 taatccatgg ttcaagtggt ccgtacatac gtaagatact gaaccaactc ttccccggta
      241 tggtttgtaa ccagttacct tctttgcctt ttggagcttt aggtccaaag tacaggtcat
      301 acgagccatc tgcatttttc ttaatctcct tgtcttgact acccactgtt ggaaatattt
      361 gatcggtttg taattgacac cttgtttgtg tatcgtatag ggttaccgcc caaaaattat
      421 ttactggaac attagttggg atgtgtaatt tataggtttt agcaccatca aaaggcttgt
      481 cgttaccgtc taacattccc aatgcataat cggaaccagc acctgcatgc gtcacagcca
      541 tagcgggtgt tacacctccg gcattataat aaaataaaac acgtgcatct aggttcatcg
      601 tgttatcggc ttcaaaggaa gtgtttttgt ttgcgtatcc cataacccag ttactctttg
      661 aatctgggta aatgtaaaca ccagctatac gagggtcata agtaatggaa cgtcctgcgg
      721 catttccgag aattgctgcc tctgtgagaa gctttttcat atttgcatca ggattgaaag
      781 gcttgccttt tacaatacca attgaagcta tctcaccacg aacctctgaa tttattgcat
      841 caagtggttc gtcttgaata atggcgttta aaatttcata atacttaagg tcacttggaa
      901 atatcgtgct aaacgatctg cccgacatgt tgatcaattc tgttggtgca ggattgtctt
      961 tatcttttaa cggatagatt ttcaatcctt ttttgaaatt gtccacagct gcttccaacc
     1021 catctttgat tgaacctctg agaaagagga aattcttatt ggtgcggggt tttagaataa
     1081 aataacctgt tggcacatca cctgtatatc ctggaggtaa taaaaggtat tttcctcctt
     1141 ttcctttgtc tggtccggtt atgcccatat cgcctacata gcgttgccat gcatcatcca
     1201 aaaagccaag cattccaggt gggatttcaa ttactgtagg tccatcttta gccaaatcgg
     1261 tactgccata cgcgtacatt gtcgaggtat tcgaagttat gactggtgtt cttgagtcca
     1321 tgagctgttc ccagatggca attttatttg gggcatcagc accttgttcg gccattcctg
     1381 aaagaacagc gtaaattgaa acggctccaa cgttatcgag ataaacatct acggcacgtg
     1441 cacgattcaa gtagtcgtaa accaaatcgt ttgtattgcc tatgggcacc ccatcaaaga
     1501 attgcaattc tccggcaacg gtttgtattt tatcaggaat tgaagctgct ttggtttttt
     1561 gttcttctcg cgatgtgttt ttgtcagtct gtgcttgcac agaaatggtt aagattgagc
     1621 tgataactac cgctacgtgt gctgttttaa ttattttttt catgatttat tttatgtata
     1681 aattaaggaa ggttggcttt ctaaaactgt tcttcttttt aaattatttt taaaatttta
     1741 tttaggaaac aacaaagtag cctgtactct taactgcgaa tcaggtccac ctgttggttt
     1801 ttcgacatta acatagtatc ctgcttgtgc attgataggt aattttccta aacgaattag
     1861 ttttcctatt ccagctccta agggaattgt ccactgttcg cctgaaggag cctcccaatt
     1921 tgctgtaata attggagctg aatttacata ccatcctttt tgtaaatttt tagtaacaaa
     1981 tacttgagag taaaagaaat tcacatctcc acgatcatca gctcctgcat aagaccagga
     2041 attttgaatt aaggttccaa ccgtccatcc tttaggttga accaataata ctacagaagg
     2101 ccctgcggtc catttttctg ctcctaagta ttgatcggtt gcagttggaa gtcctaaagc
     2161 caaaccaccg ccataaataa gttttccagg ttttgctgga gttagaaaca agcttaaact
     2221 aacatcaccc aatccaaatt cactttcacc tggaaaagtt ggttgtgata taattggtat
     2281 tattgttctt gtgattaaat tgaggttttc gtttaaagaa agtggaataa caggttgaat
     2341 gttcaaggta ttgcgggtgc gatcatcttc accgattcca aaatcggtat tattctgaaa
     2401 aggaatactg attaagcttg caattgggtt ttggatttta ctttgtaaat cgcctccagc
     2461 tgcaggttct tgttcttttt cttgtccaat tgcaaatccg ctactgatta gaaatgctat
     2521 tattaaaagg ttgcgttttt tcattgttaa tttatgttat aatactaaat atagttatca
     2581 attagttaac cttaaatact ttaggcatgt tatacgttcc attagtaagt ggagcatagg
     2641 gtccatagaa tcgtgatgtg aaacggaatc cttcagcagg tgctggtagc caatttttag
     2701 cttgttccgg atcggaaggc ttttcgtgtt ggatataaat taccaatttg ccgtctttag
     2761 gaactatgtc gccagcagct aacatgaaac tattcacagt atatctattg atttcgttag
     2821 caacgaaata gccattggag tcgtagatag ggattgacca gaactgtgtt accggaggca
     2881 aatcgttcat atcaaaagtc aaggtatatt tgttctttcc gctgaatggc ttgccatcgg
     2941 catcggtaaa caaaaatgca ccaccatgtg atacgttttt gtctggagca ccccaagctg
     3001 catcggcaat aatagcacgg tccatccatt tggtttcaaa gcctccatca ttgcggacaa
     3061 ctccccaacc attcatattg atcatatttg tttttaaagt cttacgaacg ttgtcaaatc
     3121 cagctttaaa accgtttttc aaagcagttt gctgctctgg tgacagtttg ttccattcaa
     3181 aagaaagccc ttttcctata ttgaatacct taagctgttc taccatttct gcctccattt
     3241 tcgaatcttc aatgagtggc atggttggat cgtttaaaat aacatgaagt agtgtataat
     3301 attgttcggc tgtttgtttg tcaacttgac caaactgata ctctggcaaa ccattaggaa
     3361 taggaaaatc gcctttaact atttttctat cggcttgtgg aataccttta tgtccattgg
     3421 ctaaccaatc tttcaaagga gtcatggtta actgatcttg gtagccgtta attttcttga
     3481 tttcagcgtc agtagaagtc ataagcgcca tccttgcaag accgaaagcg gtcttgaatg
     3541 gccacttgat gatatcagtt gtcgggaaat ctgctggaat attacccgta aaatctggcg
     3601 gcaccaaaat gtatttacgt gcttgtgtgc cgttgaaagg cgaaccgact atcaaccgga
     3661 aaataccata ttgatcctga acttggaaac tatagtaacg atctgttatt tctggcactt
     3721 ctacaaccac tggtccttcg cgaaggtcca taaagccagc accataaagt gttgttgcat
     3781 ttggtgtaac cactggatag tctggagtaa tttgtttacg aacccatgca aattggttta
     3841 atcctgaata aacgatattt tccttggaat cattctgcgt ataggtccaa cgttgtccgt
     3901 agtaaatagc ttgttgtagg ccataaagat aggcacgttc agcaattgct tcgagatccg
     3961 gtgtggcttc agcggttttc ttctccgttt gtgtacaact agttatcagc gtaaatacac
     4021 caataagcgt tattgcaata attttcaatt tgtttttttt catgattcta ggatttatta
     4081 atttaagaga attattattt gtaatatttt cactttcact acctcacttg gactctatct
     4141 cttgtactaa gtgttgtaga aataatctca tttcgtcttt ttttaatgtg ctgtaacg
//
LOCUS       DAOTCW010000178         4100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_17099_length_4100_cov_49.078121, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000178 DAOTCW010000000
VERSION     DAOTCW010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17099_length_4100_cov_49.078121"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..2082)
                     /locus_tag="P5335_10865"
     CDS             complement(64..2082)
                     /locus_tag="P5335_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HRZ75424.1"
                     /translation="MKRVILVVFLVVFQTMNFAQELKFGKVSIEELQETKHPLDANAP
                     AAILSKKHTVKYEYQQNIGFVQIREITMRIKIYTQEGFEYADYMAPFYNSKSARETVS
                     KVNGTTYNLEGGKITETKLKSDQVFENEVTKNRSEVKFTMPNVKPGSVLEYKYEIRSP
                     FEQSIDQVECQYEIPLNKLDVKISIPDYYTVKPSTKGYFPINLKQSKENTSFTLMSTE
                     RTNSGGTFNTVSSQTYADNYTYTSNVYSVTMSNVPAIKLEPYVDNLSNYKAAINMEVV
                     GFQFPGQLYKSYAQTWDDVVKSIYDNENFGGELKKTGYFEQDLDKVINDITIQTDRMA
                     AVLEFCKNKIKWNDYYSLFSRDGVKDAYKKGVGNSGDINLNLINMLNYAGLIANPILV
                     STKSHGIPLFPTLEGFNYVVAGVELNGSLYLLDASDKFSTVNILPERAINWSGRLIKK
                     SGGYEEVSLFPSTASQENSIVSFSLKEDAKIIGKRRVQYTSYLAKNFREKYVNVSKES
                     YLEKMESNYEGIDISNHEVTNDKELYQPVSETYDFEMANVADKIGNTIVFSPMFFLAT
                     STNPFKSETREYPIDFVYPIKSRQTFTVTIPEGYAIESIPAPLNIAMPENLGSYRYTI
                     QKTETTVQLVVQYEINKAYISQDYYDVVKDFFKQIVDKEAEKVVLKKS"
     gene            complement(2091..3986)
                     /locus_tag="P5335_10870"
     CDS             complement(2091..3986)
                     /locus_tag="P5335_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HRZ75425.1"
                     /translation="MKFKTGIIFFLLLAYSNIFSQAITDFLIPKELSENANAIVKESK
                     EKLEIINSKKMVYQVSKKIIVLNEKGDEAAASYIYYNNERNIKTLEVKVYNMMGNEIK
                     KIKKSDFKDVASQDGFSLQQDGRIKYADYTATTYPYLLHFTYSYETSDTAFLPRWTPF
                     DDYLVSVENASMEVIYDPSVGFRKSEKNFEGYTIQKAETSSSIKYTLSNLQAQKREPY
                     GLSPDEYFPKVFFALNHFSLKGYEGTASTWKELGEWFYEKLYKSQTELSPETVTKIKQ
                     LTANVATDEEKARIVYQYVQDKTRYVSIQLGIGGWQPMLAKDVDRLGYGDCKALTNYT
                     YSLLKAVGVTSYHTVIQNDIYKYDFDEDFPIIQGNHMILCIPAKDNIWVECTSQKNAL
                     GYIGRSNEDRKVLIVTPEGGQIVTTKKYLTKDNQQITKAKGKLDISGTLQVNMNRSFQ
                     GSQIDFRTHLESKTPREQEEYYRKVWDYYNSLKIKSYSTQRNQDKSTWEEQFDLEIEK
                     YASVLGNRYLIPLNVFSRDVSIPDKTRNRKQPLKILRGYVDSDEIEIEIPDGFTIEAL
                     PEPVLIQNKFGSYEAKVTMKNNIMQYNRKIQINEGDYSKEDYLLYRDFMTEINKLDNS
                     KASLIFK"
BASE COUNT         1265 a          703 c          577 g         1555 t
ORIGIN      
        1 tcatattttt gagcattaga agccaatgca aaaaataaaa aaaataacca atattttttc
       61 atgctatgat ttttttaata ctactttttc cgcttcttta tcaacaattt gcttgaaaaa
      121 atcttttact acatcataat aatcctgtga aatataggct ttattaattt catattgaac
      181 tactaactga accgttgttt ctgttttctg aatggtgtat cggtaactac ccaaattttc
      241 cggcatggca atattgagag gtgcaggaat actttctatg gcataacctt ccggaatagt
      301 aaccgtgaat gtttgtcgcg attttattgg ataaacaaaa tcgatggggt attcccgtgt
      361 ttcagacttg aacggattag tgcttgtagc tagaaaaaac attggcgaaa acactatcgt
      421 attgccaatt ttatctgcta cattggccat ttcaaaatca taagtttcag aaaccggctg
      481 atacaattct ttatcatttg tcacttcatg atttgaaatg tcaatacctt cataattact
      541 ttccattttt tctaaataac tttcttttga aacatttaca tatttttcac gaaagttctt
      601 ggccaaataa gaagtgtatt gtactcttct tttaccaatt attttggcat cttcttttag
      661 tgaaaaactt acaatactgt tttcctgaga agctgttgat ggaaacaaag aaacttcttc
      721 ataaccgccg gattttttaa tcaatcttcc actccaattg atggctcttt cgggcagaat
      781 attcacagta gaaaatttat cggatgcatc taataaatac aaactgccat ttaattcaac
      841 cccggctaca acataattaa aaccttctaa agttggaaac aaaggtatgc catgactttt
      901 cgtacttact aaaattgggt tagcaattaa tccggcgtaa ttgagcatat taatcaaatt
      961 caaattgatg tctcctgaat tgccaactcc ttttttgtaa gcatctttta caccatctcg
     1021 ggaaaaaaga ctataataat cattccattt aattttgttt ttacaaaatt ctaaaacggc
     1081 agccattctg tcggtttgaa tagttatgtc gttaattact ttatccaaat cctgctcgaa
     1141 ataaccagtc ttttttaatt ctcccccaaa attttcatta tcataaatcg attttacaac
     1201 atcatcccat gtttgagcat aacttttata taactgtccc ggaaattgaa atccaaccac
     1261 ttccatattt attgccgctt tataattgct taaattatct acataaggtt ctagtttgat
     1321 agcaggaaca ttagacatgg ttacactata aacattacta gtataggtat aattatccgc
     1381 ataagtttga cttgaaacag tattaaaagt tcctcctgaa ttagttcttt cagtactcat
     1441 cagcgtaaaa cttgtatttt ccttagattg ctttaaattt atgggaaaat agccttttgt
     1501 tgaaggttta acagtgtaat aatcaggaat agaaattttc acatctaact tattcaaagg
     1561 aatttcatat tgacattcaa cttggtcaat actttgttcg aagggtgatc gtatttcata
     1621 tttatattcc aacactgaac ccggcttcac attgggcata gtgaacttca cttctgatct
     1681 gtttttagta acttcatttt caaaaacttg atcgcttttt aattttgtct ccgtaatttt
     1741 accgccttcc aagttatagg tagtcccgtt tactttagaa accgtttctc gggcactttt
     1801 tgagttataa aaaggagcca tataatcagc atattcaaat ccttcttgcg tataaatttt
     1861 tattcgcatg gttatttccc gaatttgaac aaaaccaata ttctgctgat attcatactt
     1921 caccgtatgt ttttttgata aaatagctgc cggagcattg gcatctaacg ggtgttttgt
     1981 ttcttgaagt tcttctattg aaactttacc aaatttgagt tcctgtgcaa aattcattgt
     2041 ttgaaaaaca actagaaata ctactaaaat tactcttttc atgcgttttg ttatttaaag
     2101 attaaggatg ctttactatt atctaattta tttatttctg tcataaaatc tctgtaaagc
     2161 aaataatcct ctttagaata gtcaccttca ttgatttgta tttttctgtt gtattgcatt
     2221 atattatttt tcatggttac tttagcttca taagaaccaa atttattttg aattagaacc
     2281 ggttccggca aagcttcaat tgtaaatcca tccggaattt caatttctat ttcgtccgaa
     2341 tcaacataac ctcttaaaat tttaagcggc tgttttctgt ttcttgtttt atcaggaata
     2401 gacacatctc tactaaaaac atttaatgga atgagataac gattccctaa aacagaagca
     2461 tatttttcaa tttctaaatc gaattgttct tcccatgttg atttatcttg atttctttga
     2521 gttgagtaag attttatttt taaggaatta taataatccc aaacttttct ataatattct
     2581 tcttgctctc taggtgtttt tgattctaaa tgtgttctaa aatctatttg tgaaccttga
     2641 aaagacctat tcatgttgac ttgaagcgtg cctgaaatat ctaatttgcc ttttgcttta
     2701 gttatttgtt gattgtcttt cgtcaaatat ttttttgtag tcacgatttg acctccttcc
     2761 ggggttacta tcaacacttt tctgtcttcg tttgatcttc caatgtagcc taaagcattt
     2821 ttttgactag tacattctac ccaaatatta tctttcgccg gaatacataa aatcatatga
     2881 ttcccttgaa taattggaaa gtcttcatca aaatcatatt tataaatatc gttttgaata
     2941 acagtgtgat aggatgtaac tccaactgct tttaacaatg aatatgtata attagtaagt
     3001 gctttacaat ccccataccc aagacggtca acatctttag ctaacatggg ttgccaacct
     3061 ccaattccta attgaatgga aacatatcta gttttatctt gcacatattg ataaacaatt
     3121 ctagcctttt cttcatctgt agccacattt gcggtaagtt gtttgatttt tgtgactgtt
     3181 tcaggagata attcggtttg ggatttgtat aatttttcat aaaaccattc gcctaattct
     3241 ttccaggtag aagctgttcc ttcataacct ttcagggaaa aatgattcaa agcgaaaaat
     3301 acttttggga aatattcatc aggtgataaa ccataaggct ctcttttttg agcctgcaaa
     3361 ttggaaagtg tatattttat tgaacttgat gtttctgctt tttgaattgt ataaccttca
     3421 aaattctttt cagattttct aaaaccaacc gatgggtcat aaatcacttc catactggcg
     3481 ttttcaacac ttactaaata atcgtcaaat ggtgtccagc gaggtaaaaa agcagtatct
     3541 gatgtttcat aggaataagt aaagtgcaac aaataaggat acgtagtggc ggtataatca
     3601 gcatatttta ttctaccatc ttgttgtaat gaaaatccat cttgagaagc aacatcttta
     3661 aaatctgatt tttttatttt ttttatttca ttccccatca tattatacac ttttacttca
     3721 agtgttttga tgtttctttc gttattataa taaatataag atgctgccgc ttcatcccct
     3781 ttttcattca gaacaataat ttttttagaa acctgataaa ccattttttt agagtttata
     3841 atttctaatt tttcttttga ttctttgact atcgcatttg cattttcaga caattcttta
     3901 ggaatcagaa agtctgtaat tgcttgtgaa aatatatttg aataggctaa taaaaggaaa
     3961 aaaataatcc cggttttaaa tttcatgata aaatagatag gtttagcgaa gaaattcttc
     4021 gcctatgata aaaaataaat tgttctgctt ttcggaaatt gtggtttcca aatttaaaag
     4081 aattctgata taaaaaaaat
//
LOCUS       DAOTCW010000179         4059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_17361_length_4059_cov_32.185814, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000179 DAOTCW010000000
VERSION     DAOTCW010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17361_length_4059_cov_32.185814"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /gene="dnaK"
                     /locus_tag="P5335_10875"
     CDS             <1..1472
                     /gene="dnaK"
                     /locus_tag="P5335_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRZ75426.1"
                     /translation="TVPAYFNDAQRQATKEAGEIAGLKVMRIINEPTAAALAYGLDKA
                     GKDQKIAVYDLGGGTFDISVLELGDGVFEVLSTNGDTHLGGDDFDQVIIDWLANEFNS
                     EEGVDLRKDPMALQRLKEAAEKAKIELSSSTQTEINLPYVTATASGPKHLVKTLTRAK
                     FEQLAESLVKRSMEPVVKALKDAGLSTSDIDEVILVGGSTRIPIIQEQVEKYFGKKPS
                     KGVNPDEVVAIGAAIQGGVLSGDVKDVLLLDVTPLSLGIETMGSVMTKLIESNTTIPT
                     KKSQVFSTAADNQPSVEIHVLQGERPMANDNKTIGRFHLDGIPPAPRGVPQIEVTFDI
                     DANGIIKVSATDKGTGKSHDIRIEASSGLTPEEIDRMKKEAEINAEADKIAKEKVDKL
                     NEADGMIFQTEKQLKEFGEKLSDANKQPIEAALNELKVAYDSKDIATIQPALDKINEA
                     WKNASEEMYKAQDGQSAQQAEPTNAGGDQTQDVDFEEVK"
     gene            1605..2267
                     /locus_tag="P5335_10880"
     CDS             1605..2267
                     /locus_tag="P5335_10880"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HRZ75427.1"
                     /translation="MNKVKLLMFILFVVALTEIVAEYFRLMPLIYVFKPSISIILILL
                     YWFSSEKKDALFFLTIGTSLLTNVLFIPNDLYYIFIGLVVFIFHRFINIFYILKVMKI
                     KDFIPVLLASVPFLLVFFYIFLDTDLLTKEVYYIMIIHNILISLLGGIALSNYVMNDN
                     NRSSWLMICVLSFVTLHFIIFIEKFYIQLQIFRPIAMTLNTFAYFAFYKFILVIEKST
                     TN"
     gene            2284..2970
                     /locus_tag="P5335_10885"
     CDS             2284..2970
                     /locus_tag="P5335_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75428.1"
                     /translation="MNDFHFNKNFKIFTALFLLLTLILSFVTYEKYQSLEYYLRLICI
                     PSLMVLYFLTSEQKNKLYLFSLACATVSNAFFLSNETMFLKYGLIAFLIYRIITLIIV
                     IKKSPKLTLFSVAIGSIFFLFPLLYFIVLIEDSVGQSFFVGLINIVLVSFLGGMALTN
                     YLLENNFKHISLLTSTLLYTFLVLIFVIQKFYVFIQALDPMGIIVLMAAHYFFCVYLL
                     LAEKQKDSNQ"
     gene            complement(3048..3962)
                     /locus_tag="P5335_10890"
     CDS             complement(3048..3962)
                     /locus_tag="P5335_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HRZ75429.1"
                     /translation="MKRREFIKNTAAGTALVGFGGLSLTSFEQLKTKHLTILHTNDVH
                     SHIDPFPANHPKNANMGGVVKRAVLIEKIKQENPNVLLLDAGDSFQGTPYFNYYGGEL
                     EYKLMSKMGYEATTIGNHEFDNGIDGLAAQMPHAKFDILSANYNFENTVMNGFVKPYN
                     IFHKDGIKVGVFGLGVELAGLVDKKMYKETIYLDPVDISLDTVKKLKHEEKCDLIICL
                     SHLGYAYKDEPFKISDVKLASLTRDIDLIIGGHTHTFLDKPTIHKNLDGKDVLVNQVG
                     CYGINLGRIDFYFDSDSTVISQGKSIIV"
BASE COUNT         1322 a          611 c          667 g         1459 t
ORIGIN      
        1 ttactgttcc tgcttatttt aacgatgctc aacgtcaagc gacgaaagaa gcgggcgaaa
       61 ttgcaggttt aaaagtaatg cgtatcatca acgaacctac tgctgcagcg ttagcgtatg
      121 gtttagataa agcaggaaaa gatcaaaaaa ttgcggttta cgatttaggt ggaggtacat
      181 ttgatatttc tgttcttgaa ttaggtgatg gcgttttcga agtgttatct acaaacggag
      241 atacacactt aggaggagac gattttgacc aagtaatcat cgattggtta gcaaatgaat
      301 tcaacagcga agaaggtgta gatttacgca aagacccaat ggccttgcaa cgtttgaaag
      361 aagctgctga aaaagcaaaa attgaattat cttcttcaac gcaaacagaa atcaacttgc
      421 catatgttac tgctacagct agtggaccaa aacacttggt aaaaacatta acaagagcta
      481 aatttgagca attggctgaa agtttagtaa aacgttcaat ggaacccgtt gtaaaagcat
      541 taaaagatgc aggtttatca acttctgata ttgacgaagt gattttagtt ggaggttcta
      601 ccagaattcc aatcattcaa gagcaagttg aaaaatattt tggaaaaaaa ccatccaaag
      661 gtgtaaatcc tgatgaggtt gtggcaattg gtgctgctat tcaaggtggt gttttaagtg
      721 gtgatgttaa agatgtattg ttgttagacg ttactccttt atcattagga attgaaacaa
      781 tgggaagtgt aatgaccaaa ttaattgagt ctaacacaac catcccaacc aaaaaatcac
      841 aagttttctc tacagcggca gataaccaac catcggttga aatccatgtg ttgcaaggag
      901 aaagaccaat ggcaaatgac aacaaaacaa ttggtcgttt ccatttagat ggaattccac
      961 ctgctccaag aggtgtgcct caaattgaag ttacttttga tattgatgcc aatggtatta
     1021 tcaaagtttc ggctactgat aaaggaacag gaaaatcaca cgatattcgt atcgaagctt
     1081 cttcaggatt aactccggaa gaaatcgaca gaatgaaaaa agaagcagaa atcaatgcag
     1141 aggctgataa aattgctaaa gaaaaagttg ataaattgaa tgaagccgac ggaatgattt
     1201 tccaaacaga aaaacaactt aaagagtttg gtgaaaaatt atcagatgct aataaacaac
     1261 caattgaagc agcattaaac gagttgaaag ttgcttatga ttcaaaagat attgcaacaa
     1321 ttcaacctgc tttagacaaa atcaatgaag cttggaaaaa tgcttctgag gagatgtaca
     1381 aagcacaaga tggtcaatcc gctcaacaag ctgaaccaac aaatgcaggt ggagatcaaa
     1441 cgcaagatgt agattttgaa gaagtgaaat aatctatcaa aacaatattt caaaaccgag
     1501 ttagcaatag ctcggttttt ttattttata ataattcaac taaaaattat caactggtta
     1561 aaaatttcat aactttattc ggaaatcaac aataaatagt caatatgaat aaagtaaagt
     1621 tattgatgtt tatactgttt gtggtggctt taactgaaat tgttgccgag tatttcagat
     1681 taatgccttt gatttatgta ttcaagccat ctatctcaat tatactgatt cttctttatt
     1741 ggtttagttc tgaaaagaaa gatgctttat tttttctaac tattggaact tctttgctaa
     1801 caaatgtttt atttattcca aacgatttgt attatatttt cattggttta gtagttttta
     1861 tttttcatcg atttatcaat attttttata ttttgaaagt catgaaaatt aaagatttta
     1921 ttcctgtctt gttggcttcg gtaccttttt tattggtgtt tttttacatc tttttagata
     1981 ctgatttgtt aactaaagag gtttattata ttatgattat tcataatatt ctgatttctt
     2041 tacttggagg aattgccttg tctaattatg tcatgaacga taacaatcgt agttcttggt
     2101 taatgatttg tgttttgtcg tttgtaacgt tacactttat tatctttatt gaaaaatttt
     2161 atattcaatt acaaattttc agacctattg ctatgacgct taatacgttt gcctattttg
     2221 ctttttataa atttatattg gttattgaaa aatcaacgac taattaaatt ttatttactt
     2281 tttatgaatg actttcactt taataaaaat tttaaaatct tcactgcatt atttcttttg
     2341 ctgactttga ttttgtcatt tgtgacttat gaaaaatacc aatcgttgga atattattta
     2401 aggttgattt gcattccttc tttaatggtt ttgtattttc tcacctctga acaaaaaaac
     2461 aaattgtatt tattttcatt ggcttgtgca actgtttcaa atgccttttt tctttccaac
     2521 gaaaccatgt ttttaaaata tggtttgatt gcttttttaa tttacagaat cattacatta
     2581 ataattgtaa taaagaaatc accaaagctt actctttttt cagtagcaat tggtagtata
     2641 tttttcttat ttccactctt atattttatt gtattgattg aagattctgt tgggcaaagt
     2701 ttttttgtag ggttgataaa tatcgtatta gtttcttttt taggaggaat ggcactcact
     2761 aattatttat tggaaaataa ttttaaacat atttcgctat tgacaagtac gttgctgtat
     2821 acctttttag tgctgatttt tgtgattcaa aaattttatg tgtttattca agcattagat
     2881 cctatgggaa taattgtttt gatggcagct cattattttt tctgcgttta tttattgttg
     2941 gctgaaaaac aaaaagactc gaatcaataa atttgattat ttaaaccaaa agaatgattc
     3001 gtttttgata gttattattt tttgtggtat atgtctctgt tagaacatca gacaattatc
     3061 gatttacctt gtgaaattac cgttgaatct gaatcgaaat aaaagtctat tcgtcctaaa
     3121 ttaattccat agcaacctac ttgattcact aaaacatctt tcccatccaa atttttatga
     3181 attgttggtt tatctaagaa agtgtgagta tgtccgccaa ttatcaaatc aatatcacgg
     3241 gttagggatg ctaattttac atcgcttatt ttaaaaggtt catctttata ggcataacct
     3301 aagtgggaca aacaaataat taaatcacat ttttcttcat gtttgagttt ttttaccgtg
     3361 tccaacgaga tatcaaccgg atcaaggtaa attgtttctt tatacatttt tttgtcaact
     3421 aaccctgcta attcaactcc taaaccaaat acgcccactt taattccgtc cttgtgaaag
     3481 atattataag gttttacaaa accattcatt acagtatttt caaagttata attagcggaa
     3541 agaatgtcaa atttggcatg aggcatttga gcagctaaac catcaattcc gttgtcaaat
     3601 tcatgatttc cgattgtggt tgcctcgtaa cccatttttg acattaattt gtattccaat
     3661 tcaccgccgt aataattaaa atagggtgtt ccttgaaaac tatcaccggc atctaaaagt
     3721 aaaacatttg gattttcttg tttgattttt tcaattaaaa cagctctttt tacaacacca
     3781 cccatatttg cattttttgg atgattagca ggaaatggat cgatatggct atgaacatcg
     3841 ttcgtatgca atatggttaa atgtttggtt tttagttgtt caaaactagt caatgacaat
     3901 cctccaaatc ccactaaagc agttccggca gctgtatttt ttatgaattc tcttcttttc
     3961 attaactatt ttaatttgga ttgataataa ttctcggaac agacgaagct tcaaccgtgt
     4021 taacctcttt aaagtaatca atcagtatat ttctaattt
//
LOCUS       DAOTCW010000180         4057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_17381_length_4057_cov_38.731134, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000180 DAOTCW010000000
VERSION     DAOTCW010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17381_length_4057_cov_38.731134"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..1860
                     /gene="pelF"
                     /locus_tag="P5335_10895"
     CDS             313..1860
                     /gene="pelF"
                     /locus_tag="P5335_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HRZ75430.1"
                     /translation="MKRKHDVMLILEGTYPFNGGGVSTWAHMLCNEVKNSNFTLYSIN
                     AEFESKSKYKLSENVTNVIQVPLWSPMEPQEMIDYGKKYYKIISLKEKNDLEKINQVF
                     IPIFQDLLRHIFSNNRDVRAFDDTIRKMWKYFQKHDYKKTMKSEPVWKTFCYMVSDLN
                     SESENEKVTLYDLTSGMRWIYRFLIPLSVEVPRVDVSHLTLSGFPVIPALVLKYKYGT
                     PMIVTEHGVFIRERLLAINSSEYSYFLKKMLIKFSECVTELVYYKADKILSVNKFNIK
                     WEKMYGADPEKIEIIYNGIDHNVFKPLQKPAHLENIPTVVAAARIFDLKDIVTMIKSC
                     AVAKKQIPNIQYLVYGDNNAVPEYTAECEKLIDELDLRNNFKLAGYHSKPHELFCEGD
                     ISILTSISEGFPYTVLESMSCGIPVVATDVGGVTEALDNSCGYICKPKDFEEIGNRVV
                     ELLNDKALREKMGENARKKVIENFTIGKFIAEYEDVYERVITREVKEVQMPILIPSME
                     EEQVKAS"
     assembly_gap    2021..2120
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2121..2900
                     /locus_tag="P5335_10900"
     CDS             <2121..2900
                     /locus_tag="P5335_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75431.1"
                     /translation="AKTYIYFFFRNYLKNHPPKADLNIKSSVFIYQNHRYFLYGLFVY
                     FFIFIDRILAWSANTNGQLPFVIYFEKDYELGMDMAILVFLLLAGVLEFSIASFTRFL
                     DITQKLVQFEKPKNFNKELHKLYWQNILVLLITSLIIYFLIYQVMFASWGYKGQFNED
                     LQNISIKVCYLGGTGYFFLAWGMLNTLYLFTLGHTQQPLKGIIYAIVINITVGFILSR
                     FLSFEYSVVGMLIGALFFMLYTLKHIFHFFKKLDYHYYASF"
     gene            2887..3705
                     /locus_tag="P5335_10905"
     CDS             2887..3705
                     /locus_tag="P5335_10905"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HRZ75432.1"
                     /translation="MRRFNLFLWFLPSLIGSPVFSFPVNKSTVLVCYGKLDAAKIKGY
                     NYVILEEKHFKKEEITIIKKNNKNVLAYISLGEVNSNASHYNLLKNNTLGKNNNWNSH
                     YLDLKSDKTTIVLMRLIKKNLDKGFDGLFLDNIDNYSSFGPQKNQRKELIELIKKIKK
                     QYPNHLLIQNSGAELIEDTNKSVDAILFESVATNFSFKDNVYKLREIKDYTAYLERLK
                     AIESKYKLPIILVEYADNQKLYSEVINRLASTNFEYFIGKIDLQSIPNFTKTIK"
     gene            3702..>4057
                     /locus_tag="P5335_10910"
     CDS             3702..>4057
                     /locus_tag="P5335_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75433.1"
                     /translation="MILSSTILIGFVRLILLLLFLYYCNNKLVKRQESHTLIQFIVNE
                     WFKYGSLSIIIMFVLVQIQIYSLINFSLILLFLILIDVVGIRNLKNPVKYFKEVSKSR
                     FFEFIKNVENKKSIKE"
BASE COUNT         1414 a          519 c          638 g         1386 t
ORIGIN      
        1 tagaataagt tactcgtcta gagcactatg catttcgtcg atgaaatgag tgttttcaac
       61 agatttcagc gtttttattt tgtgctcact agatttaaat ctaaatttgt aaagaattta
      121 atgctagaaa gtatttatta atattatgtt aatcagcatt ttcaaacttt aagttctttt
      181 tagaagtcgt ccccaaaaag ccggcgttcc aaaaaatacc acaaaccacc ctaagattgt
      241 taaagtgcac caacttgtct ggttctgcat aggcagaatt atcggcgaac ggacctttat
      301 tatgtaatta ttatgaaaag aaagcacgat gtaatgttaa ttttagaagg gacttatcct
      361 tttaacggtg gtggagtctc cacatgggca catatgcttt gcaatgaagt taaaaattct
      421 aattttacgc tttattctat taatgctgaa ttcgaatcaa aatccaaata taaacttagt
      481 gaaaatgtca ctaatgttat tcaagttcct ttatggtctc caatggagcc acaagagatg
      541 attgattatg gtaaaaaata ctataaaatt atttccctaa aagaaaaaaa tgatttagaa
      601 aaaatcaatc aagtattcat tccaattttc caagatttat tgcgtcatat cttttctaat
      661 aacagagatg ttagagcttt tgatgatact atccgtaaaa tgtggaaata ttttcaaaaa
      721 cacgattata aaaaaacaat gaaaagtgaa ccggtttgga aaactttttg ttacatggtt
      781 agcgatttga attcagaaag tgaaaatgaa aaagttactt tgtatgactt aacttcagga
      841 atgcgttgga tttacagatt tttaattccg ctttctgttg aagttccaag agttgatgtt
      901 tctcacttaa cattatcagg ttttccggtt attccagctt tagttttgaa atataaatat
      961 ggaacaccaa tgattgttac agaacacggc gtttttattc gtgagcgttt attagcaatc
     1021 aactcttctg agtattctta ttttttgaaa aaaatgttga ttaaattctc agaatgtgta
     1081 acagaattag tttactataa agcagataaa attctttccg ttaataaatt taatattaaa
     1141 tgggaaaaaa tgtatggagc tgatccggaa aaaattgaaa ttatttataa tggtatagat
     1201 cacaatgtgt ttaaaccgct acaaaagcca gctcatttag aaaatatacc aacggttgtt
     1261 gccgctgccc gtatttttga tttgaaagat attgttacga tgattaaatc gtgtgcagtt
     1321 gccaaaaaac aaatccctaa tattcagtat ttagtttatg gtgataataa tgctgtaccg
     1381 gaatatactg cagaatgtga aaaattaata gatgaattag atttaagaaa taattttaaa
     1441 ttagcaggtt accacagtaa accacatgaa ttattttgtg aaggtgatat atctatctta
     1501 acttctattt ctgaaggatt tccatataca gttttggaat ctatgagttg tggaattcct
     1561 gttgtagcta ctgatgtagg tggtgttact gaagcattgg acaattcctg tggttatatt
     1621 tgtaagccaa aagattttga ggaaattgga aatagagtag tagaactgct taatgacaaa
     1681 gccttgagag aaaaaatggg agaaaatgca agaaaaaaag tgattgagaa ctttacaata
     1741 ggtaaattta ttgctgaata tgaagatgtt tacgaaagag taattacaag agaagtaaaa
     1801 gaagttcaaa tgccaatttt aatcccatct atggaagaag aacaagtaaa agcctcataa
     1861 accaacccaa gcccaaaccc aacttaaccg aaaaatatag aatatttgtg ttctaaaaaa
     1921 tgcaaatgga aaagcatagt gaaaacataa aaaacttaat caataatgtc aaagataaaa
     1981 taggtaaacc ggtcagcagt agagttgttg ccgctaccat nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn gctaaaacgt atatttattt tttctttaga aattatttga
     2161 aaaaccatcc accaaaagcg gacttaaata ttaaatcctc cgtttttatt tatcaaaacc
     2221 accggtattt cttatatggt ttattcgttt atttctttat ttttattgat agaattttag
     2281 cttggtccgc caatacaaat ggtcaacttc cttttgttat ttattttgaa aaagattacg
     2341 aattaggaat ggatatggca attttggttt ttctactact tgcaggagtg ttagagttca
     2401 gtattgcctc ttttacaagg tttttagata ttacccaaaa attagttcaa tttgaaaagc
     2461 ccaaaaactt taataaagaa ctgcacaaat tatattggca aaacattttg gttttattaa
     2521 taaccagttt gataatttat tttttaattt atcaagttat gtttgcttca tggggttata
     2581 aaggccagtt taatgaagat ttacaaaaca ttagtataaa agtttgttat ttaggtggaa
     2641 ctggttattt tttcttagct tggggaatgc ttaatacctt gtatttgttt actttaggac
     2701 atactcaaca gccattaaaa gggataatat atgcgattgt aataaatata accgttggtt
     2761 ttattttgag ccgcttttta agttttgagt acagtgttgt tggcatgtta ataggagctc
     2821 tgttttttat gttatatact ttaaaacaca tatttcattt ttttaaaaag ttagactatc
     2881 attattatgc gtcgttttaa tttgtttttg tggtttttgc catcattaat aggctcacct
     2941 gttttcagtt ttcctgtgaa caagagtact gtattggttt gttatggcaa acttgatgca
     3001 gcaaaaataa aagggtataa ttatgtgatt cttgaagaaa aacattttaa aaaagaggaa
     3061 ataactatca ttaaaaagaa taataagaat gtattggcct acattagttt aggtgaagta
     3121 aatagtaatg cttctcacta taacttatta aaaaacaata ctttaggaaa aaataataat
     3181 tggaacagtc attatttaga tttaaaatca gataaaacaa ctattgtttt gatgcggtta
     3241 atcaagaaga atttagacaa aggttttgat ggattatttc ttgataatat tgataattat
     3301 tcttcttttg gtcctcaaaa aaaccagcgg aaagaattaa ttgaattaat aaaaaaaatt
     3361 aaaaagcaat atcccaatca cttattgatt caaaattccg gagccgaact tatagaggat
     3421 acaaacaaat ctgttgatgc aattctattt gagtcagttg caacaaattt cagttttaag
     3481 gataatgtat acaaactaag agaaataaag gattatacag cttatcttga gcgtttaaaa
     3541 gcgatagaat ctaagtataa attgccaatt attttggttg aatatgctga taatcaaaag
     3601 ttatattcag aagttattaa tcgccttgct tcaactaatt tcgagtattt tattggaaaa
     3661 attgacttac aatcaattcc aaatttcacc aaaactatca aatgatactt tccagcacca
     3721 tattaatcgg ttttgtgcga ctcattttgt tgttgctttt tctatattat tgcaataata
     3781 aattagtaaa aagacaagaa agtcataccc tgattcaatt catcgtgaat gaatggttta
     3841 aatatgggag tttgtccatc atcattatgt ttgtcttagt acaaatacaa atttacagtt
     3901 taataaactt ctctttgatt ttgctttttt taatattgat agatgtagtt ggaattagaa
     3961 atttaaaaaa cccggttaag tatttcaaag aagtatctaa atctcgtttt tttgaattca
     4021 ttaaaaatgt ggaaaacaaa aaatccatca aagaaat
//
LOCUS       DAOTCW010000181         4051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_17414_length_4051_cov_22.261011, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000181 DAOTCW010000000
VERSION     DAOTCW010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17414_length_4051_cov_22.261011"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..834
                     /locus_tag="P5335_10915"
     CDS             199..834
                     /locus_tag="P5335_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HRZ75434.1"
                     /translation="MIHFSKDTIEQLPKIPRLNLINSCTGYKSANLIATKTNEGVSNV
                     AIFSSITHLGSDPAMLGFILRPTTVPRNTYKNIKDVGYFTVNHITETMIIDAHHSSAS
                     YDENISEFNQTSLEEEYYDGISFPFVKNSPVQILCKYLNEYEIKENGTLHIIASIEQI
                     IIKENLIQKDNWLRLDLENVVTINGLDGYCVPKLVDRFEYARPNVPTKSML"
     gene            837..983
                     /locus_tag="P5335_10920"
     CDS             837..983
                     /locus_tag="P5335_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HRZ75435.1"
                     /translation="MAHKKVNHPEKICKTCGLPFSWRKKWEKNWVEVLYCSEKCRRNK
                     NSNT"
     gene            1002..2282
                     /locus_tag="P5335_10925"
     CDS             1002..2282
                     /locus_tag="P5335_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASH family cryptochrome"
                     /protein_id="HRZ75436.1"
                     /translation="MNNVVWFRNDLRVNDNYSLFQACKKSGKVIGVYFLDPRQFEMTR
                     YGFKKTEKFRAKFLLETLRDLKNNLAKKNISLLIFHDLPESVFPKLVNDLQISTIFLQ
                     KEWTSEEEKIKSNVKNQISKVDFVETYDQFLFHPEDIPYAYFSEIPEVFTVFRKKCEA
                     TVKVRQTVSIEKQPDSNWIEPNSFNITLEDLGLESFEIHPNSAFPFRGGESSAMKRLE
                     NYFFETENLSSYKQTRNGLIGAEYSSKLSAWSANGSISARTIYWKILQYEQEICKNED
                     TYWLIFELIWRDYFKYISLKHGNKIFQLDGILGKKYDWKFHSKAFEQWKNGTTNEPFV
                     NANMVELQQTGFMSNRGRQNVASYWAKEWEQDWQVAAAYFESMLIDYDVHSNYGNWMY
                     NAGVGNDPRDRKFNIKRQAEMYDAKGEYQRLWLK"
     gene            2373..3905
                     /locus_tag="P5335_10930"
     CDS             2373..3905
                     /locus_tag="P5335_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HRZ75437.1"
                     /translation="MKTLRLLLGDQLNHNHSWFHQTDENVIYLMAEMRQETDYVKHHI
                     QKVVAFFLSMRNFASHLKSKKHQLVYFEIDDAKNLQHLEKLILKTIESYSIEKFEYLL
                     PDEFRLDEQLKQICQNLPVPTEAFDTEHFYTTRTELTDFFQGKKLLLMENFYRMMRKK
                     HNVLMIGANPVGNQWNFDHDNRKKYKGEVPIPKAKIFPKNVKEIIHQLEKMEVNTFGS
                     IDAENFNWSTSREECLEQLDYFCQHLLKHFGDYEDAMHVEEKFLFHSRLSFAMNSKML
                     SPKEVIEHVVHYYHQKEDEITLSQVEGFVRQILGWREYVRGIYWKEMPNYSSMNALEN
                     YNKLPDFYWTGKTKMNCMQKAISQSLEEAYAHHIQRLMIIGNFSLLTQMHPDEVDAWY
                     LGVYIDAIEWVEMPNTRGMSQYADGGILATKPYVSSGSYINKMSNYCGSCHYNVKEKL
                     GEKACPFNSLYWNFLDDKKEHFKNNQRMAMMLNLLKKIPAKELYGIKLNVKEVMENLD
                     AI"
     gene            complement(3898..>4051)
                     /locus_tag="P5335_10935"
     CDS             complement(3898..>4051)
                     /locus_tag="P5335_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRZ75438.1"
                     /translation="LPTGQSGNPLSEHYKDQAELYNQGKFRKMKMNKKEIEASSRKLI
                     FKPKSK"
BASE COUNT         1433 a          629 c          760 g         1229 t
ORIGIN      
        1 ttccaactaa tccggaacca acaacagcaa tcttttgagg ggtttgcata aatgactttc
       61 aaaaattcga ttacaaaaat agcgattata ataggaattc ccgatttgag aagaatatat
      121 ttcagctaaa ctaaaaatat tgtttaactt ttaatatatt ttattaaaca aaaactatct
      181 ttgaaaaaaa ttaatttaat gattcatttt tcaaaagata caattgaaca gctaccaaaa
      241 attccacgat tgaatttaat caatagttgt accggctata aatcggcgaa tttaattgca
      301 acgaaaacca atgaaggtgt ttccaatgtt gccattttta gcagtatcac ccatttggga
      361 agcgatccgg cgatgttagg atttattctt cggccaacca ctgttcccag aaatacctat
      421 aaaaacatca aagatgtagg ttattttact gtcaatcaca tcactgaaac catgataatt
      481 gatgctcatc attcttccgc aagttatgat gaaaatatct ccgaatttaa ccaaactagt
      541 ttggaagaag aatattatga cggaatatcg tttccctttg taaaaaatag tccagtgcaa
      601 atactctgca aatacctgaa cgaatatgaa ataaaagaaa atggaaccct tcatattata
      661 gcttcaattg aacaaatcat tatcaaagaa aacttgattc aaaaagacaa ttggttgcgt
      721 ttggatttag aaaatgtagt tacgattaat ggattagacg gttattgtgt accaaaatta
      781 gtagatcgat ttgagtatgc tcgcccaaat gttccaacaa aatcaatgct ctaaaaatgg
      841 ctcataaaaa agtaaatcac cctgaaaaaa tatgcaaaac ctgtggactt ccattttctt
      901 ggcgaaaaaa atgggagaaa aactgggtag aagttttgta ttgcagtgag aaatgtagaa
      961 gaaataaaaa ttcaaatact taaattccaa attccaatta aatgaataat gtcgtttggt
     1021 ttcgaaatga tttacgagtg aatgataact attcactttt tcaagcatgt aaaaagtcgg
     1081 gaaaagtaat aggagtttac tttctggatc cgagacaatt tgaaatgacg cgttacggat
     1141 tcaaaaaaac agaaaaattc agagctaaat ttctactgga aacacttcgg gatttgaaaa
     1201 ataatttggc aaaaaaaaat atttcgttgc tcatttttca tgatttacct gaaagtgtat
     1261 ttccaaaact ggtgaatgac ttacagattt caaccatttt tcttcaaaaa gaatggacaa
     1321 gtgaagaaga aaaaatcaaa agtaatgtca aaaatcaaat ttcaaaagtt gattttgtag
     1381 aaacctacga ccaattttta tttcatccgg aggatattcc gtatgcttat tttagtgaaa
     1441 ttccggaagt ttttacggtc ttcagaaaaa aatgcgaagc aactgtaaaa gtcagacaaa
     1501 ctgtttcaat tgaaaaacaa cctgattcca attggattga accaaattct ttcaacatta
     1561 ctttagaaga tttaggtttg gaaagttttg aaattcatcc caattccgct tttccttttc
     1621 gtggcggcga atcaagtgca atgaaacgat tagaaaatta tttttttgaa actgaaaatt
     1681 tatcatccta taaacaaacc cgaaacggat taattggagc cgaatacagt tcgaaacttt
     1741 ctgcttggtc ggcaaatggt agtatttctg caagaacaat ttattggaaa attctgcaat
     1801 acgaacaaga aatttgcaaa aatgaagata cctattggtt gatttttgaa ttgatttggc
     1861 gggattattt caaatacatt tcactgaaac acggcaataa aatttttcaa ttggatggca
     1921 ttttaggaaa aaaatacgat tggaagttcc attcaaaagc ttttgaacaa tggaaaaacg
     1981 gcactaccaa tgaaccattt gtaaacgcca acatggttga attacaacaa accggattca
     2041 tgagtaatcg tggacgtcaa aatgtagcaa gttattgggc caaagaatgg gagcaagatt
     2101 ggcaggtggc agcagcttac tttgaaagta tgttgattga ttatgatgta cactccaatt
     2161 atggcaattg gatgtataat gccggagtgg gtaacgaccc gagagaccga aaattcaata
     2221 ttaaacggca agcggaaatg tatgatgcga aaggggaata tcagagactt tggttgaaat
     2281 aaatttgttt caggtttcac gttatcccga cttgaaactt gaaacctgaa actacaaaaa
     2341 ataatgagat tgcttcgtgc ctcgcaatga ctatgaaaac attacgccta cttctcggtg
     2401 atcaactaaa tcacaaccat tcttggtttc atcaaaccga tgaaaatgtc atttatctga
     2461 tggcagaaat gcgtcaggaa accgattatg taaaacatca cattcaaaaa gtggtggctt
     2521 tctttttgag tatgcgaaat tttgcttcgc atttaaaaag taaaaaacat cagctggttt
     2581 attttgagat tgatgatgca aagaatcttc aacatttaga aaaattgatt ctaaaaacca
     2641 ttgaaagtta ttccattgaa aaatttgaat acctattacc ggatgaattt cggttggatg
     2701 aacaattaaa gcaaatttgt caaaatcttc ctgtcccaac agaagcattt gacaccgaac
     2761 atttttatac aaccagaacc gaattaaccg attttttcca agggaagaaa cttttgttga
     2821 tggaaaactt ctatcgcatg atgcgaaaaa agcacaatgt tttgatgatt ggagccaatc
     2881 cggttgggaa tcaatggaat tttgaccacg acaatcggaa aaaatacaaa ggcgaagttc
     2941 cgattccgaa ggcgaaaatt tttcctaaaa atgtgaaaga aattatccat caattggaaa
     3001 aaatggaggt caataccttt ggttcgattg acgcagaaaa ttttaattgg tcgacttctc
     3061 gtgaagaatg tttggaacag ctagattatt tctgtcagca cttgttgaaa cattttggcg
     3121 attatgaaga tgcgatgcat gtggaagaaa agtttctttt tcattcccgc ctgtcgtttg
     3181 cgatgaattc gaaaatgctt tcgccgaagg aagtaattga acatgttgtg cattattatc
     3241 atcaaaagga agatgaaata acactttcac aagtagaagg ttttgtgcgg caaattttag
     3301 gttggcgaga atatgttcgg ggaatttatt ggaaagaaat gcctaattat tccagcatga
     3361 acgcattgga aaattataat aaattgcctg atttttattg gacgggaaag acgaaaatga
     3421 attgcatgca aaaagcgatt tcgcaaagtt tggaagaagc gtatgcacat catattcaac
     3481 gtttaatgat tattggcaac ttttctctac ttacgcaaat gcaccccgat gaagtagatg
     3541 cttggtattt gggtgtttac attgatgcga ttgaatgggt agaaatgccc aacacacgcg
     3601 gcatgagtca atatgccgat ggtggaattt tagccaccaa accttatgtt tcttcgggaa
     3661 gttacatcaa caaaatgagt aattactgtg gaagttgcca ctataatgta aaagaaaaac
     3721 taggtgaaaa agcgtgtccg ttcaactctt tgtattggaa ttttttggat gataaaaaag
     3781 agcatttcaa aaacaaccaa cggatggcga tgatgttgaa tttgttgaag aagattccgg
     3841 cgaaggaatt gtatgggatc aaattgaatg tgaaggaggt tatggaaaat ttggatgcta
     3901 tttagatttt ggtttgaaaa ttaacttccg agaactcgcc tcaatttcct ttttattcat
     3961 tttcattttt cggaatttgc cttgattgta cagttctgcc tggtctttgt aatgttcact
     4021 taacggattt ccggattgtc cagttggtag a
//
LOCUS       DAOTCW010000182         4000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_17760_length_4000_cov_29.074018, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000182 DAOTCW010000000
VERSION     DAOTCW010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17760_length_4000_cov_29.074018"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="P5335_10940"
     CDS             <1..533
                     /locus_tag="P5335_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRZ75439.1"
                     /translation="VNKLQNQVTNYKMRNELYYIKAPQNGYVNRALQSGLGETIKEGT
                     PVVSIMPSKYDIAVETYIDPIDLPLIHKGEKVRIWFDGWPTIVFSGWPNTSYGTFGGS
                     IVAVENFISTNGKYRILIAQDPSDKPWPKQLSIGAGAQTFALLDNVPIWYELWRKLNG
                     FPPDFYTPKQTNESKK"
     gene            541..1941
                     /locus_tag="P5335_10945"
     CDS             541..1941
                     /locus_tag="P5335_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRZ75440.1"
                     /translation="MRTIIACFLLFSVYSWSQNKASDSILTLDAYLGYVKQFHPLVKQ
                     ANIEVSQAQAGIIASRGGFDPKIEVDYENKQFKSTEYYDLLNTTFKIPTWYGIEIKAG
                     FDQMDGVYLNPQNTTPDNGLAILGISVPLGQGLLINNRMADLKKAKLYAKLSQAERDL
                     EVANVLYLASNSYFHWYKNYNEYKLYETFLKNAKVRYDGITSLISAGDRPAIDSVEAG
                     ILVKSRQLSLEQSRLKLVKSKLELSNYLWFDNNLPLELQDFITPEATLEKTVENTLKT
                     NGLLLANIDFENHPKIKSLQNKVSILEVDKRLKGNLLLPTINLNYNYISEPEFVEDFN
                     TLNYKYGVNFSFPLFLRKERGNFKIAKFKLQDAELDLQFENQVLKNKIEYQLEEISSL
                     KNQINIANDLVANFQTMLDSEEKLFLFGESSLFIINSRENSLLNTSLQQIDTQVKFCV
                     SNSDLFKLIANPEFNN"
     gene            complement(1993..3279)
                     /locus_tag="P5335_10950"
     CDS             complement(1993..3279)
                     /locus_tag="P5335_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRZ75441.1"
                     /translation="MSKYVTAEEAVKVVKSGDRVYLQAAAASPTVLSKALTNRASELR
                     NVEICHLHTEGEAPYANPELADSFHVNSFFLGANVRHTLKAGNGSYTPVFLSELPVLF
                     RKNVLPLDVVFIHVSPPDRHGYCSLGVSVEASVAAIENAKIVIAQVNPNMPRTFGDGI
                     LHESEIDYLVEVNEPIFSHGIDIFSPEEEKIGEFVASLIEDRSTLQMGIGSIPNAALS
                     KLHNHKDLGLHTEMFSDGVIDLIEKDIINCNYKGTTRGRVLATFLMGSRRLYDFVDDN
                     SFVELKESSAVNDTARIRKNPKMVAINSAIEVDLTGQVCADSIGGKMYSGVGGQMDFI
                     RGASLSVGGKAIIALPSQTKRGESRIVPFLKQGAGVVTTRSHVQYIITEYGIANLYGK
                     TLKQRVTEMVKIAHPNHREWIEKAYYEMSEIKPFTR"
     gene            3448..>4000
                     /gene="map"
                     /locus_tag="P5335_10955"
     CDS             3448..>4000
                     /gene="map"
                     /locus_tag="P5335_10955"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRZ75442.1"
                     /translation="MIIPKTREEIELMRESALLVSRTLGEIAKEIKPGVTTLYLDKLA
                     EQYIRDNGGIPGFLGLYGFPNSLCMSPNAQVVHGIPNNKPLEEGDVISVDCGVLKNEF
                     YGDHAYSFEIGEVAPDVKKLLKVTKESLYVGIRQFKAGNRVEDVGNAIQTYCESHGYG
                     VVRELVGHGLGRKMHEDPEMPNYG"
BASE COUNT         1388 a          669 c          687 g         1256 t
ORIGIN      
        1 aagtgaataa attgcaaaat caggttacta attacaagat gcgtaacgaa ctttattaca
       61 taaaagctcc acaaaatggt tatgtaaacc gtgcgttgca atccggtctt ggcgaaacga
      121 ttaaagaagg aactccggtt gtaagcatta tgccttctaa atatgatatt gctgtggaaa
      181 cctatattga ccctattgat ttgccactaa ttcacaaagg agaaaaggtt agaatttggt
      241 ttgatggatg gcctaccatt gtttttagtg gatggccgaa cacttcctac ggaacttttg
      301 gaggatccat cgttgctgtt gaaaatttta tcagcacgaa tggaaaatac cgtattttga
      361 ttgctcaaga tccatcagac aaaccttggc ctaagcaact aagtattggt gcgggagctc
      421 aaactttcgc tttattagac aatgtaccaa tttggtatga actgtggcgg aaattaaatg
      481 gatttccacc tgatttttat acaccaaaac aaaccaatga atcaaaaaaa taaggaaatc
      541 atgagaacaa taattgcttg ctttttgctt ttttctgttt attcttggtc gcaaaacaaa
      601 gcttctgata gtattttaac gttggatgcc tatttaggct atgtcaaaca attccatccc
      661 ttggtgaaac aagctaatat tgaagtgagc caagctcaag caggaattat tgcaagtcgc
      721 ggtggatttg atccaaaaat agaagtggat tacgaaaaca agcaatttaa atcgactgaa
      781 tattatgatt tattgaatac caccttcaaa attcccactt ggtatggaat tgaaataaaa
      841 gcaggttttg atcaaatgga tggtgtgtat ttgaatccgc aaaacactac tcccgataac
      901 ggattagcaa tacttggaat ttcggtgcct ttaggtcaag gcttacttat taataatcgc
      961 atggccgact taaaaaaggc aaaattatat gcaaaattat cgcaagcaga acgtgatttg
     1021 gaagtagcta atgttctcta tttagcctct aatagttatt tccattggta caaaaactat
     1081 aatgaataca agctatatga aactttttta aaaaatgcaa aagtaagata tgatggtatt
     1141 acaagtttaa tatctgccgg tgaccgaccg gcaattgaca gtgttgaagc aggaattttg
     1201 gtaaaatcca gacaattaag tttagaacaa tctcgattaa aattagttaa atcgaaatta
     1261 gaactttcga attatttgtg gtttgacaat aatttaccgt tagaattgca agattttatt
     1321 actcctgaag ctacgttgga aaaaacagtt gaaaatacgt taaaaactaa tggattattg
     1381 cttgctaaca ttgattttga aaatcatcca aaaataaaat cacttcaaaa taaagtcagc
     1441 atactagaag tagacaaacg gctcaaaggg aatctgttat taccaaccat taatttaaat
     1501 tacaattaca tatcggagcc tgaatttgtt gaagatttta atacgttgaa ttataaatat
     1561 ggtgtaaatt ttagcttccc tttatttcta agaaaagaaa gaggtaactt taaaatagcg
     1621 aaattcaaat tgcaggatgc tgaattggat ttacagtttg aaaatcaggt tttgaagaat
     1681 aaaatcgaat accaattgga agaaattagt tcgttaaaaa atcaaataaa cattgcaaat
     1741 gatttagtag caaatttcca aacaatgtta gattctgaag aaaaactatt tttatttgga
     1801 gaaagttctt tatttataat caattctcgt gaaaacagtc tactcaacac cagtttacaa
     1861 caaattgata cacaagtaaa gttttgtgtc tcaaactccg atttatttaa attgattgca
     1921 aatccggaat ttaataatta aaaaactgct ttgtttatat gaatttaaac aaagcagtta
     1981 agaactattt ttttaacgag taaatggttt gatttcactc atttcatagt atgctttttc
     2041 aatccattcc cgatgattcg ggtgagcaat tttaaccatt tctgtcactc tttgtttgag
     2101 agttttgcca tacagatttg caattccata ctctgtaata atatattgaa cgtgggatct
     2161 agtagtaaca acacccgctc cttgtttcaa aaaaggcacg attctcgatt cacctctttt
     2221 tgtctgagaa ggtaaagcaa tgattgcttt tcctccaaca cttaaggaag cacctcgaat
     2281 aaagtccatt tgaccaccaa caccggaata catttttcca ccaattgaat cggcacaaac
     2341 ctgtccggtt aaatcaactt caatagcgga attaatagca accattttcg gatttttacg
     2401 aattctggcg gtatcattca ctgctgatga ctcttttaat tctacaaatg aattatcatc
     2461 aacaaaatca tatagccgtc gagaacccat caagaaagtt gccaaaacac gtccacgtgt
     2521 ggttccttta taattacaat tgatgatgtc tttttcaatt aaatcgatta ctccatccga
     2581 aaacatttca gtatgcaatc ctaaatcttt gtgattatgc aacttactca aagcagcatt
     2641 tggtattgat ccaataccca tttgaagggt gcttctgtct tcaattaatg aggctacaaa
     2701 ttcccctatc ttttcttctt ccggagaaaa tatatcaatc ccatgagaga aaataggttc
     2761 attaacttcc accaaataat ctatttcaga ttcatgtagg attccgtcac caaaagttct
     2821 aggcatattt ggatttactt gagcaatcac aatttttgca ttttcaattg ccgctaccga
     2881 agcctcaaca gaaactccca aggaacaata tccatgtcga tcgggaggag aaacatgaat
     2941 aaaaacaaca tctaagggca aaacattttt tctaaataaa acaggtaatt cactcaaaaa
     3001 aacaggcgta tatgagccat ttccggcttt taaagtatgt ctgacatttg ctcctaaaaa
     3061 aaacgaatta acatgaaaac tatcagctaa ttcaggattt gcatagggag cttcaccctc
     3121 ggtatgcaag tgacatattt ctacatttct taattcggaa gctctattgg ttaatgcttt
     3181 ggataaaacg gtaggcgaag cagcagctgc ttgtaaataa actctatctc ctgattttac
     3241 aacttttaca gcctcttctg cggttacata tttgctcata ttctaaaatt ttcaacaaat
     3301 ataaaatgaa tttcatttaa caaagatgat atttctcatt gtttattttt ttttggttcg
     3361 aaaaggtgtt cgtgccaaaa gtaaagctac taaacaaaat aataaaaaca acaaaaaagt
     3421 gtacctttgt ccctttaatt tttgaaaatg attatcccaa aaacaagaga agaaattgaa
     3481 ttaatgcgtg aaagtgcatt gttggtttct agaacattag gtgaaattgc aaaagaaatt
     3541 aaacccggtg ttacgacatt atatctagat aaattagctg agcaatacat ccgagacaat
     3601 ggtggtatac ctggtttttt aggattatat ggtttcccaa attcactttg tatgagccca
     3661 aatgctcaag tagttcatgg tatcccaaac aacaaaccat tagaagaggg cgatgttatt
     3721 tcggtagatt gtggtgtttt aaaaaatgaa ttttacggag accatgcgta ttcgtttgaa
     3781 ataggcgaag tagctccgga cgtaaaaaaa ttattaaaag ttactaaaga atcactttat
     3841 gttggtatcc gccaatttaa agcaggtaat cgagtggaag acgtgggaaa tgctattcaa
     3901 acttattgtg aatcgcatgg ttatggagtt gtgagagaat tagtaggtca tggattaggc
     3961 agaaaaatgc atgaagatcc ggaaatgcct aattatggca
//
LOCUS       DAOTCW010000183         3992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_17801_length_3992_cov_41.230886, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000183 DAOTCW010000000
VERSION     DAOTCW010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17801_length_3992_cov_41.230886"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3301)
                     /locus_tag="P5335_10960"
     CDS             complement(<1..3301)
                     /locus_tag="P5335_10960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRZ75443.1"
                     /translation="MNRLKHYIVLPFLLWSLLPSLLFSQNIRFKHLTINDGLSQNAIL
                     AMVQDSDGFMWFGTKDGLNKYDGYHFTIYQNEPSNRKSISSNYITALFTARNGQVWIG
                     TDNGILNVYSKTTNDFQRILLPLALSETKNIKEITSITQDSSGTMWIGTYGNGIFKIP
                     TVNNNPAISKIKQITISKNQKDVISNKIKALQFDNSGILWIGTENGLKRFDIQKELFT
                     TFLINIKQPGAYGSKEDFLISSIAVADKNHLWLGTVSGLVHFNTENFSQETYLHHLSV
                     FSYGWGQINKIVADKKGYLWLATPGELMRFDTITKKYESFKNDPLKPETISYDNIHSL
                     CIDRTGIVWAGTNGMGIDYYDPKVNRFALVTRIASLNSRISGFSIRSIVEQDDRYVWI
                     GSNVLYRWDRKTDELKSFETNSQRPNDFGNTGISSMIKSKDQKIWFASIEGLFVYDCK
                     SMKTIHYTPTKNNEKGLPRKDVTAVFEDKKGEIWIVTENCLSKLNNREKGEFKHYPYR
                     SNSGLNVMSRTAFYDDGKDNLYIGTKGGLVIFNTKKETYHTYQNIPEISNSLSNNIVN
                     SICPDIENPNRYLWIGTSGGLNLFDVNQKTFKQYTQKDGLPNNVIYGILSDSQHNLWL
                     STNKGLSKFNLKTKKFRNYDVADGLQSNEFNTGAYFKSKKGELFFGGIKGMNYFFPEQ
                     IVDNPFEPQIRIAGLKVYSEGTTTRDGTEIKELLVNPIDKMTFTHRDDMIVFDFAALD
                     FSAPEKNKYAYKLENFNDDWIYTDYSRTATFTNLPPGNYTLKVKGSNNDGIWNEKGIT
                     IPIKVLPHWTGTWWAYTLYFILLLLLLYFIRKYEMTRIKMKNKLELESKEFDTLKVLD
                     QMKSRFFTNISHEFRTPLTLISGYAENLIEELPTNSTKKQVVAIDQNAKTLLKLINEL
                     LDISKLEAGKMFLCSTQQNFVLYLKHLFFSAESFAELKKIKLNFISDEEDIQVSFDAE
                     KMEKIIMNLLFNALKFTPEGGEITLTIKQNNLHSVSICICDNGIGMTEEAISHIFNRF
                     YQADNSDTRLYEGTGIGLALVKELIELHQGSIIAHRNENLTGQNGATFTIELPIGVIS
                     EKEIALPVETET"
     gene            complement(3497..>3992)
                     /locus_tag="P5335_10965"
     CDS             complement(3497..>3992)
                     /locus_tag="P5335_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75444.1"
                     /translation="KVISLFILLLSFNSFSQEVFDYEFDDNVTIQVVEDAEEGEIPGG
                     KYVKGTYKNEVIVFSSSDKGKDKLSNTDEAGLLKFFQGVKDGAVKSSKGKLLNEEIIT
                     LHDLKALKFSFTLDVNGKKNIVENYVFVYKNLAYTLQFMDTEKGFDKLTDFRTKIVNS
                     IVLK"
BASE COUNT         1238 a          694 c          622 g         1438 t
ORIGIN      
        1 ttgtttccgt ttcaaccggc aacgcaattt ctttttcaga aattacgcca ataggtagtt
       61 ctatcgtgaa tgtcgctcca ttttgtcccg ttaagttttc gtttcgatgg gcgataatcg
      121 atccttggtg caattcaatt aattctttta ctagtgccaa tcctattccg gttccttcat
      181 ataaacgggt gtctgaatta tccgcttgat agaagcgatt gaatatatga gaaatagctt
      241 cctccgtcat tccaataccg ttgtcacaga tacagatgga tacggaatgc aaattatttt
      301 gtttaattgt taaggtaatt tcaccacctt cgggagtaaa tttcaaagca ttaaaaagga
      361 gattcatgat aatcttttcc attttttcag catcaaaaga aacttgtata tcttcttcat
      421 cggaaataaa gttcagtttg atttttttca actcagcaaa tgattctgcg gaaaagaata
      481 aatgttttag atataaaaca aaattttgtt gcgtgctgca cagaaacatt tttccggctt
      541 ccaattttga aatatctaac aattcgttga ttaatttcaa aagtgttttg gcattttgat
      601 caatggcaac cacttgtttt ttagtactat tggttggcaa ttcttctatt aaattttcgg
      661 cataaccgct aatcaaggtt aaaggtgttc taaactcatg agaaatattt gtaaaaaacc
      721 tcgatttcat ctgatccaaa acttttaagg tatcaaattc tttactttcc agttctaatt
      781 tgtttttcat tttaatccgc gtcatctcat attttctgat aaaatagagt agaagaagca
      841 ataaaataaa atagagggta taagcccacc aagtcccggt ccaatgtggc aaaaccttaa
      901 tcgggatggt gattcctttt tcattccaaa taccatcatt attgcttcct tttaccttaa
      961 gggtataatt tccgggtggt aaattggtaa aagttgccgt tctggaatag tccgtataaa
     1021 tccaatcatc attaaaattt tctaatttat aagcatattt atttttttca ggagctgaaa
     1081 aatcaagagc tgcaaaatca aacacaatca tatcgtcacg atgtgtaaat gtcattttat
     1141 ctatcggatt aacgagtaat tctttaattt cagtgccatc tcttgttgtt gtaccttcac
     1201 tgtagacttt tagacctgca attctaattt gtggttcaaa gggattatct acaatttgct
     1261 caggaaagaa ataattcatt cctttaattc caccgaaaaa aagctcccct tttttacttt
     1321 taaaataggc tcctgtgtta aactcattgc tttgtaaacc atctgccaca tcataattcc
     1381 taaatttttt tgttttcaga ttaaatttag aaagcccttt gttggtgctc aaccataaat
     1441 tatgctgtga atctgaaaga attccataaa tcacattgtt gggcaaacca tctttttgtg
     1501 tatactgttt aaatgttttt tgattaacat caaataaatt taaaccgcct gacgtgccaa
     1561 tccataaata gcgattgggg ttttcaatat ccggacaaat tgaatttact atattattgc
     1621 ttaggctgtt tgatatttct gggatatttt ggtacgtgtg ataggtttcc ttttttgtat
     1681 taaaaatgac taatccccct ttagtcccaa tatataaatt gtcttttcca tcatcataaa
     1741 atgccgttcg actcatcaca tttaaaccgg aattactacg gtaaggatag tgtttaaatt
     1801 ctcctttttc gcgattgttc agcttactga gacaattttc ggtaacaatc caaatctcgc
     1861 cctttttatc ctcaaatact gcggtaacat ctttacgagg taatcctttt tcattatttt
     1921 tagtaggagt ataatgaatc gttttcattg atttgcaatc atatacaaat aatccttcga
     1981 tggatgcaaa ccaaattttt tgatcttttg atttaatcat ggacgaaata ccggtatttc
     2041 cgaagtcatt cggtcgttga gaatttgttt caaaactttt caattcatcc gtttttctgt
     2101 cccaacgata caacacattg ctgccaatcc aaacgtaacg gtcatcttgt tcaacaatag
     2161 agcgaatact gaaacctgaa attctggaat ttaaacttgc tattcgtgta actaaagcaa
     2221 aacgatttac tttcgggtca taataatcaa tccccattcc gttagtgccc gcccaaacaa
     2281 tgcctgttcg atcaatacat aaactatgga tattgtcata actaatcgtt tccggtttta
     2341 atgggtcatt tttaaaagat tcatattttt tggttattgt atcaaaacgc attaattcgc
     2401 cgggtgtggc tagccataaa tagccttttt tgtcggctac aatcttatta atttgtcccc
     2461 atccgtaact aaaaacactt aaatgatgca aataggtttc ttgggaaaaa ttttcggtat
     2521 taaaatgaac caatccactt acggttccca accataaatg atttttatca gcaacggcaa
     2581 tgcttgaaat taaaaaatcc tctttgcttc cgtaggctcc gggttgcttt atattaatta
     2641 aaaaagtagt aaacagttct ttttgtatat caaatctttt cagaccgttt tcagtgccaa
     2701 tccataagat accggaattg tcaaactgta acgctttaat tttattactt attacatctt
     2761 tttgattctt tgaaatagta atttgtttga tttttgatat ggcaggatta ttattgaccg
     2821 ttggaatctt aaaaattcca tttccatatg tcccaatcca cattgtaccg gaagaatctt
     2881 gtgtgatgga agtgatttcc tttatgtttt tagtttctga taaagcgagt ggaagtaaaa
     2941 tacgttgaaa atcattggta gtttttgaat aaacgtttag tattccatta tcagttccaa
     3001 tccatacttg tccatttcgg gcggtaaaca gagccgtaat gtaatttgat gaaatggatt
     3061 tccggttgga aggttcattt tggtaaatgg taaaatgata gccatcatat ttattcagtc
     3121 cgtctttggt gccaaaccac ataaaaccat cgctatcctg caccatggca aggattgcat
     3181 tttgagacaa accatcatta atagtaaggt gtttaaaacg aatgttttga gaaaagagta
     3241 gggaggggag caaactccac aaaagaaatg gtaatactat ataatgcttt aatcgattca
     3301 tttatcgtta ttgattgcac gtaaataggg cgataaatcg atttgtggaa attaaactaa
     3361 cacgtattac ggtgatagaa taaataaaag taaacaatat ttgggaaaaa caatcgtatt
     3421 aagttaaaaa aagaagatta acgttttaaa aattagatta aactcggata taaatttcct
     3481 tttttattaa tagttgttac tttaatacaa tggaattgac aatttttgtt ctaaaatcgg
     3541 ttagtttgtc aaaacctttt tcggtgtcca taaactgtaa cgtataagcc aggtttttat
     3601 aaacgaatac ataattttcc actatatttt tttttccatt tacgtctaaa gtaaatgaaa
     3661 attttaaggc tttgaggtca tgaagtgtga taatttcctc gtttaggagt tttcctttgc
     3721 tggatttgac agctccatct ttcacacctt gaaaaaactt tagtaaaccg gcttcatcgg
     3781 tattgcttaa tttatctttt cccttatcag aactggaaaa aacaattact tcatttttat
     3841 acgttccttt tacatatttt ccacccggaa tttcaccttc ctcagcatct tccacaactt
     3901 gaatcgttac attgtcatca aattcatagt caaaaacttc ttgactgaaa ctgttgaaac
     3961 ttaataatag aataaatagg gagattactt tt
//
LOCUS       DAOTCW010000184         3946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_18115_length_3946_cov_45.070162, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000184 DAOTCW010000000
VERSION     DAOTCW010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18115_length_3946_cov_45.070162"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..>3946
                     /locus_tag="P5335_10970"
     CDS             195..>3946
                     /locus_tag="P5335_10970"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRZ75445.1"
                     /translation="MLLIAAVISFAYIFPATSQNIVINEVLASNTAVNKDEDDSYQDW
                     IELYNTGITPINLQGYGLTDDSTELFKWVFPNVTINPGSYLLIWASDKNRVNPLNPLH
                     TNFKISGDGETMSLTEPLGTVVDFSPAAPMLANISYGRSPNGSGDFVYFQLPTPNLEN
                     IGTGYTETLSPPQFSQNSGFFSSGFNLMLSSVDEGVTILYTLDGSEPDENNLSGTTYS
                     YKNQYPKLPGQAFGTFLYNSFQTLQYSLPIPIIDRSPLPNKVSMISSTYDFIPPYLPE
                     NPIFKSTVVRAKVIKPGALASPVVTKNYFISPSANNEFTLPIVSLCIDENKLFDYNNG
                     IYVAGLDFDTWRTNNPTDVADYEIGNFARKGATSERISNFSYFVNGSEVINQNIGIRI
                     RGNYSRVYPSKSFNFYAKTEYGDEDLDYTFFSDQTDNSYTRLSLKNSSGDFYHTMFRD
                     PLNHELIKGLRVETEAYQPTITFINGEYYGILSLREKYDDKYFKREYGIESDQLDVLE
                     NNAIEEEGDNEHYLEMIEYIEDHSLAPLNHYNYVKTQMDTENFQDYFISNIFFQNVDW
                     PGNNIIYWRKKTAQYEPTALYGLDGRWRWAIHDMDSTFGISGGSISLNSLANATAEDG
                     PDWPNPEWSTLILRKLLENNSFKNDFINRFADLMNTYFHSDRIVEKINSMKTVLEPEM
                     PKQFDRWKAPQDFGDWNYFLNREIDFANQRPGFQRTHIRDEFSIATNIDVTLNVSDQD
                     HGFIKINTIDVLSTTPGINTNPYPWTGIYFSNIPVKLKAIANEGYVFSHWEGISNSTE
                     QEIIIDSAISFSITAVFIPQGFSVEVSEPIYFWLMNSALPNDTPITNINSTFEVLNDG
                     ILAYQSCLEGYPFTNGHPNWRKASLERRNSPTNINYIPSANGNIPFSTSIMRGIQVKQ
                     PFQNNGLENALLFNFSTVGYKNIVFGFAAKDEDAADAIIVDYSVSAGNPVWLTTDLSS
                     ISLSLSSEFQLFEVDFSAIINANNNPNFKIRLRFTGSNMTVDNGDRVTFNNFSVKGVQ
                     LPLQYFTPNVYTIGTTITALSPTVVANVDSYSIVPSLPNGLSFDESTGIISGTPTELS
                     PATDYEVTATNSGGSTSFTLSIEIIDSAPTNLSYSTPNVFIIDEAITNLVPMVSGENL
                     EFSIEPSLPNGLSFDETTGIISGTPTELSAATNYTVTASNSGGSTSFVLSIAVIDQAP
                     MSLVYSTPNIFTIDEAITDLVPMVSGENLEFSIVLSLPNGLNFDPIT"
BASE COUNT         1317 a          631 c          678 g         1320 t
ORIGIN      
        1 atctgatatt aagaaaataa taaaaagata actattttaa atcagttttt tcaaaaaaag
       61 gaggctttta tgcctccttt tctttttgtc gataatatat gttttttatc gataaaatga
      121 tagagtaagt ttttaattaa actactttta taaaaaacaa ttccatcctc ctaatatttt
      181 agaatatgaa aaaaatgctg ctaatcgccg ccgtgatttc ttttgcctat atctttccag
      241 ctactagcca aaatattgtg attaatgaag ttttggcctc caatacagct gtaaataaag
      301 atgaagatga cagttatcaa gattggattg aattgtataa tactgggata actcccataa
      361 atcttcaagg atatggttta acagatgatt caaccgaatt atttaaatgg gtttttccta
      421 atgtgacaat taatccgggt agttatcttt taatttgggc atctgataaa aacagagtaa
      481 atccgcttaa cccacttcac actaatttta aaattagtgg cgatggcgaa acaatgtctt
      541 taactgaacc tttaggaact gttgttgatt tctctccggc tgctccaatg ctagctaata
      601 tttcctatgg aaggtctcca aatggtagtg gagattttgt ttattttcaa cttccaacgc
      661 ctaatttgga aaatatagga acgggatata cagaaacact tagtccgcct cagttctctc
      721 aaaattcagg atttttcagc tcagggttta atttgatgct ttcttctgtt gatgaagggg
      781 ttacaatttt gtatacttta gatggctcag aaccagatga aaataattta tcaggaacga
      841 cttatagtta taaaaatcaa tacccaaaac ttccgggtca ggcatttggc acatttttat
      901 acaactcatt tcaaacttta caatattctt taccaatacc aattattgat agatcaccct
      961 taccaaataa agtgtcgatg atttcttcta catatgattt cataccacct tatttaccag
     1021 aaaatccaat ttttaaatca acagtagtta gagcaaaagt gataaaaccg ggtgctttag
     1081 cgagtcctgt tgtcactaaa aattatttta tttctccttc agctaataat gaatttaccc
     1141 ttccaatagt gtcattatgt attgatgaaa ataaattatt tgattataat aatggaattt
     1201 atgtagccgg tttagatttt gacacttgga gaacaaataa tccaacggat gttgcggatt
     1261 atgaaatagg aaatttcgcc cgaaaaggtg caacttctga aagaatctca aatttcagtt
     1321 attttgtgaa tggttcagag gtaataaatc aaaatattgg aattcgaatt cgtggaaatt
     1381 attctagggt ttatcctagt aaatcattta atttctatgc taaaacagaa tatggagatg
     1441 aggatttgga ttatactttc ttctctgacc aaacggataa tagttatacg cgactttctt
     1501 taaaaaattc aagtggcgat ttttatcaca ccatgtttcg cgatcccctg aatcatgaat
     1561 taattaaggg attaagagta gaaaccgaag cttatcaacc aacaattaca ttcataaatg
     1621 gagaatatta tggaatatta agtttgcgtg aaaagtatga tgataaatac tttaaaagag
     1681 aatacggaat tgaaagtgac caattagatg ttttagaaaa taatgccatt gaagaagaag
     1741 gggataatga acattatctt gaaatgattg aatatattga agaccattct ttagctcctt
     1801 taaatcatta taattatgtt aaaacgcaaa tggatactga aaattttcag gattatttca
     1861 tttccaatat ctttttccaa aatgtagatt ggccagggaa taatattatt tattggagaa
     1921 agaaaacagc tcaatatgaa ccaactgctc tgtatggttt agatggaaga tggcgttggg
     1981 caattcatga catggattca acttttggaa ttagcggagg gagtatttct ctgaattcat
     2041 tagctaatgc aacggctgaa gatggtcctg attggcctaa tccagaatgg tcaacgttga
     2101 tactaagaaa gctattggaa aataattctt tcaaaaatga ttttatcaat cgttttgcgg
     2161 atttaatgaa tacttatttt cattcggata ggattgttga aaaaattaat tcaatgaaaa
     2221 ccgttttaga acctgaaatg ccaaaacaat ttgaccgttg gaaagctcct caagattttg
     2281 gtgattggaa ttatttttta aatagagaaa ttgattttgc taatcaaaga ccaggatttc
     2341 aacgaacgca cattcgtgat gaatttagta ttgcaacaaa tattgatgtt accctgaatg
     2401 tttcagatca agaccatgga ttcatcaaaa taaacacaat tgatgtcctt tctacaactc
     2461 caggaattaa tacaaatcca tatccatgga ccggaattta tttttcaaat attcctgtaa
     2521 aattaaaagc catagccaat gaaggctatg tttttagtca ttgggaaggt atttctaaca
     2581 gcacagaaca agaaattatt attgattcag ctattagttt ttctataaca gcagttttta
     2641 ttcctcaagg gttttcagtt gaagtgagtg aaccaattta tttttggtta atgaattctg
     2701 ccttgccaaa tgatactccc attacgaaca ttaattccac atttgaggta cttaatgatg
     2761 gaattttggc ttatcaatct tgtttggaag gttatccttt taccaatggg catcctaatt
     2821 ggagaaaagc ctctctggaa agaagaaata gtccaacaaa cataaattat ataccttcag
     2881 ctaacggaaa catccctttt tcaacttcta ttatgagggg aattcaagta aaacagccat
     2941 tccaaaacaa tggtttagaa aacgctttgc tattcaattt ttcaacagta ggctataaaa
     3001 atatagtttt tggatttgct gcaaaagatg aagatgctgc agatgcaatt attgtagatt
     3061 attcagtttc tgccggtaat ccggtttggt tgacaactga tttgagctct atttctttgt
     3121 ctctttcctc tgaatttcaa ttatttgaag ttgatttttc tgccatcatt aatgcaaata
     3181 ataatccgaa ttttaaaatt agattacgtt ttacgggttc aaatatgact gtagataatg
     3241 gagatcgtgt aacttttaat aatttttctg tgaagggagt tcagttacct ttacagtatt
     3301 ttactcccaa tgtttataca attggaacaa caattactgc tctaagtcca acagttgtag
     3361 caaatgtaga tagttatagt attgtaccaa gtttaccaaa cggattaagt tttgatgagt
     3421 caaccggaat tatctcaggc acaccgacag aactttcccc ggccacggat tatgaggtta
     3481 cagcaaccaa ttcaggtgga agcactagtt ttacactatc aatagaaatc atcgatagtg
     3541 ctccaactaa tttgagttac tccacgccaa atgttttcat aatcgatgag gctatcacga
     3601 atttggttcc catggtttcg ggagaaaatc tagaattttc catcgaacca agtttaccaa
     3661 acggattaag ttttgatgaa acaacgggaa ttatctccgg cacaccgact gaattatcag
     3721 cggcaacgaa ttacacagta acggcaagta attccggagg aagtacaagt tttgtgttgt
     3781 cgattgcagt gatagatcaa gctccaatga gtttggtgta ttcgactccg aatatcttta
     3841 ccattgatga ggccattacg gatttggttc ctatggtttc aggagaaaat ttagaatttt
     3901 ccatcgtact aagtttacca aacggattaa attttgaccc aataac
//
LOCUS       DAOTCW010000185         3887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_18509_length_3887_cov_48.088987, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000185 DAOTCW010000000
VERSION     DAOTCW010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18509_length_3887_cov_48.088987"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="P5335_10975"
     CDS             complement(<1..378)
                     /locus_tag="P5335_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRZ75446.1"
                     /translation="MNFLDITIGILLVFSLYNGIKNGVFVEIASIISFILGVFLAIKF
                     SYLVKSFLEGFVSWNPQTIQITAFILTLIFVVIGVHLLAKIFTKIADFAFLGWVNKLA
                     GGIFSVLKTALLIGIFLNLVQKIN"
     gene            500..856
                     /locus_tag="P5335_10980"
     CDS             500..856
                     /locus_tag="P5335_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75447.1"
                     /translation="MSRDIQLKERWENVVQLLSNQFAEGEILDLDAIIYLIGVQELGK
                     VKQTFKKDEKIELMHIAICRLLEPYGYYEFEFFDNDGWPHYKVKEVLPPLKAGEQAVL
                     MKDAIVNYFLERELIN"
     gene            941..>1948
                     /gene="pheS"
                     /locus_tag="P5335_10985"
     CDS             941..>1948
                     /gene="pheS"
                     /locus_tag="P5335_10985"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRZ75448.1"
                     /translation="MIDKIKEYIGEAQAFQTDNKETLEQFRIKYLGSKGLLKDLFAEF
                     KNVPNEQKKEFGQVINTLKNTAEEKVKSILELLESKEETVGLFGDLTRPGEPFPIGSR
                     HPISLVKNQIIDIFSNIGFNVSEGPEIEDDWHNFTALNLPEYHPARDMQDTFFIQTNP
                     DVLLRTHTSSVQVRYMENNKPPIRTISPGRVFRNEAVSSRSHCIFHQVEGLYIDKDVS
                     FADLKQTLLYFTKEMFGKSKIRLRPSYFPFTEPSAEVDIYWGLKTETDYRITKGTGWL
                     EIMGCGMVDPNVLKNCGINPDEYNGFAFGMGIERIAMLLYQIGDIRMFYENDVRFLEQ
                     FK"
     assembly_gap    1949..2048
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2049..2438
                     /locus_tag="P5335_10990"
     CDS             <2049..2438
                     /locus_tag="P5335_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75449.1"
                     /translation="IPKVENVFMAAVQEWSDDFMDKVWYVYLVNDSDFDLDSVMVVSK
                     AFGTIKGEMKKTSLLRHAFIKVPSVSVIKVEMVEQSVLALNNEFMVTYFIGNTLYDKK
                     FIFRTNTINERATEEVPILFVNGVIVK"
     gene            complement(2441..2659)
                     /locus_tag="P5335_10995"
     CDS             complement(2441..2659)
                     /locus_tag="P5335_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75450.1"
                     /translation="MSNRWKFQLKIGFYWGTFMTLFMLLFYSKEVAVIDQIKSSQFYL
                     KSIGHFLIGIFIMGYIWWKEKNIASKKD"
     gene            complement(2660..3655)
                     /locus_tag="P5335_11000"
     CDS             complement(2660..3655)
                     /locus_tag="P5335_11000"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRZ75451.1"
                     /translation="MNNSPKFAVIGGGSWATAIAKMLCENQTEICWYMRNQTAVDHIK
                     AEKHNPNYLSSVEFNTNKLKLTTDINEAVAYADYLIFAIPSAFLNTELEKLTESFEHK
                     VIFSAIKGIVPETFLIVGEHFHKQYNIPYENIGVITGPCHAEEVALERLSYLTIACAD
                     AKKAKVVAQNLSCKYIKTKISDDIIGTEYAAMLKNIYAIAAGMAHGLGYGDNFQALLM
                     SNSIREMKKFIKKVHRMKRNINDSAYLGDLLVTGYSVFSRNRMFGNMIGKGYTVKSAM
                     MEMSMVAEGYYAVKSAYKLNQNYQAKTPIIDAVYEILYEGKDPKSVFKKLTDKLD"
     gene            complement(3682..>3887)
                     /locus_tag="P5335_11005"
     CDS             complement(3682..>3887)
                     /locus_tag="P5335_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRZ75452.1"
                     /translation="IIEQDCWGEVFNAVAPFHPSRKRYYTEKAIEYNLAFPQFNENLT
                     SIGKTISSDKLIRILGYEFKNLG"
BASE COUNT         1283 a          627 c          628 g         1249 t
ORIGIN      
        1 atttatcttt tgaactaaat tcaaaaaaat tccaattaac aaagctgttt tcagcacact
       61 aaatattccg ccggctaatt tgtttaccca acctaaaaaa gcaaaatcgg ctattttagt
      121 aaatattttt gctaataaat gaactccaat aactacaaaa attaaagtca atataaaagc
      181 agtaatttga atggtttgcg gattccaact cacaaaacct tctaaaaaag atttcactaa
      241 ataggaaaat ttaattgcaa gaaaaacacc caatataaac gaaataattg aagctatttc
      301 tacaaaaaca ccatttttga ttccattgta taaactaaaa acaagcaata ttccaattgt
      361 aatatctaaa aaattcattt ctaatttttt actaaaatag taatttagat ttaatcaata
      421 tcaacttctt atctttgcaa aaaaaagcag ttgattggtt agtcaaattt tccaatattc
      481 aaccgttaac ttcaaggcta tgtcaagaga tattcaatta aaagaacgtt gggaaaacgt
      541 tgtccaacta ttatcaaatc aatttgcaga aggcgaaata cttgatttag atgccattat
      601 atatttaatt ggtgttcaag agttgggtaa agtaaaacaa actttcaaaa aagacgaaaa
      661 aattgagtta atgcacattg caatttgtcg attattagag ccttatggtt attatgaatt
      721 tgaatttttt gataatgatg gttggcctca ttataaagta aaagaagtgt taccgccact
      781 aaaagcaggc gaacaagccg ttttgatgaa agacgccatt gtaaattatt ttctggaaag
      841 agaactaatc aattaattca ttttattttg agatcggaca agttgcgact agtccctatt
      901 aataaatttt ccctaaattt gtagctttat agcacgaacg atgatagata agattaaaga
      961 atatattggc gaagctcagg cttttcaaac agacaacaaa gaaactttag aacaattccg
     1021 aataaaatat ttaggaagta aaggcttgtt gaaagacttg tttgccgagt ttaaaaatgt
     1081 tcctaatgaa cagaaaaaag aattcggaca ggttatcaac acactaaaga atacggccga
     1141 agagaaagta aaatctattt tagaattact ggaaagtaaa gaagaaaccg ttggtctttt
     1201 tggcgatttg actcgtccgg gagaaccttt tccgataggt tcccgtcacc cgatttcttt
     1261 agtaaaaaat caaattatcg atattttttc caatataggg ttcaacgttt ctgaaggtcc
     1321 tgaaattgaa gacgattggc ataatttcac cgccttaaat ctcccggaat atcatccggc
     1381 acgagacatg caggatacgt tttttattca aaccaatccg gatgttttat tgcgaacgca
     1441 cacgtcttcg gttcaagtgc gatatatgga gaacaacaaa ccgcctattc gaacgatttc
     1501 acccggaaga gttttcagaa atgaagcagt atcttctcgt tcgcattgta tttttcatca
     1561 agttgaagga ttatatattg acaaagatgt ttcgtttgcc gatttaaaac aaacactttt
     1621 atatttcacc aaagaaatgt ttggtaagtc aaaaattcgt ttgcgaccat cttactttcc
     1681 atttactgag ccaagtgctg aggtagatat ttattggggt ttaaaaaccg aaacagatta
     1741 ccgtatcact aaaggaaccg gctggctaga aattatgggc tgcggaatgg ttgatccaaa
     1801 cgttttgaaa aactgtggta tcaatcctga cgaatacaac ggttttgctt ttggaatggg
     1861 aattgaacgt attgctatgt tattatacca aattggcgac attcgtatgt tctatgaaaa
     1921 tgatgtgcgt tttttagaac aatttaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnat cccaaaagtt gaaaatgtat ttatggccgc tgtacaggag tggagcgatg
     2101 actttatgga taaggtttgg tatgtttatc ttgtaaacga cagtgatttt gatttagata
     2161 gcgtaatggt tgtttccaaa gctttcggaa cgataaaagg agaaatgaaa aaaacatctt
     2221 tgctgcgtca tgcttttatc aaagttccat ctgtttcagt aataaaagtg gaaatggttg
     2281 agcaaagtgt cttggcactt aacaatgaat ttatggtgac ttattttatt ggaaacacgt
     2341 tatacgataa aaaatttatt ttcagaacca acactattaa cgaacgggct actgaagaag
     2401 tgcctatact gtttgtgaat ggtgtaattg taaagtaaat ttagtctttc ttcgaagcaa
     2461 tatttttttc tttccaccaa atataaccca taataaagat tccaatcaaa aagtgtccaa
     2521 tggatttcaa atagaattga gacgatttaa tttgatctat aacagcaact tctttgctgt
     2581 aaaataacag catgaagagt gtcataaatg ttccccaata aaagcctatt tttaattgaa
     2641 atttccagcg gttactcatt taatccagct tatccgtcag ttttttaaac acacttttag
     2701 gatctttccc ttcgtataaa atttcataaa ctgcatcaat aattggtgtt tttgcttgat
     2761 agttttgatt cagtttataa gcacttttca cggcataata accttccgca accatgctca
     2821 tttccatcat ggcacttttt acggtatatc cttttccaat catgtttcca aacattcgat
     2881 ttcgggaaaa tacagaataa ccggttacta ataaatcgcc taaataagca gaatcattaa
     2941 tattccgttt catgcggtgt acttttttga taaacttttt catttcacga atggaattac
     3001 tcatcaataa agcctgaaaa ttgtcgccat atcctaagcc gtgagccata ccggctgcga
     3061 tggcataaat attttttaac atagccgcat attccgttcc gataatatca tcggaaattt
     3121 ttgttttgat gtatttacaa gataaatttt gagccaccac ttttgctttt tttgcatcag
     3181 cacaggcaat tgtcaaatac gaaagacgtt ctaaagcaac ttcttccgcg tgacacggac
     3241 cggtaataac accaatattt tcataaggaa tattgtattg tttgtgaaaa tgttcaccaa
     3301 cgattaaaaa tgtttccgga acaatccctt tgatggcaga aaaaatgact ttatgttcaa
     3361 atgattctgt taatttttct aattcagtgt ttaaaaaggc ggaaggaatg gcaaaaatca
     3421 ggtaatcggc ataagcaacc gcttcattta tatcagtagt aagctttaat ttattggtgt
     3481 taaattcaac agaactcaaa tagtttggat tgtgtttttc tgctttgatg tggtcaaccg
     3541 cggtttgatt tcgcatatac caacaaattt ctgtttggtt ttcgcaaagc atttttgcta
     3601 tcgcagttgc ccagctacca ccaccgatga cagcaaattt tggtgaattg ttcattttaa
     3661 gtttagtttg gaaaggtaaa tttaacctaa atttttaaat tcatatccca aaattcgaat
     3721 caatttatcg gaacttattg tttttccaat tgaagtcaga ttttcattaa attgcggaaa
     3781 agctaaattg tattctatcg ctttttctgt ataatatctt tttcgggaag gatgaaaagg
     3841 agccacagca ttaaaaactt caccccaaca atcctgttct attattc
//
LOCUS       DAOTCW010000186         3882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_18542_length_3882_cov_48.731382, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000186 DAOTCW010000000
VERSION     DAOTCW010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18542_length_3882_cov_48.731382"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1134
                     /gene="gldA"
                     /locus_tag="P5335_11010"
     CDS             238..1134
                     /gene="gldA"
                     /locus_tag="P5335_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HRZ75453.1"
                     /translation="MSIEVKNISKIYGDQKALDNISFSVKNGEIVGFLGPNGAGKSTL
                     MKILTTYITADEGTASVNGLDVISSKKEVQRIVGYLPEHNPLYLDLYVREYLAFNADV
                     YKIDKSRIQEVIELTGLTPESTKKIGQLSKGYRQRVGLATALLHNPEVLILDEPTTGL
                     DPNQLVEIRELIKNIGKDKTVFLSTHIMQEVEAICDRVIIINHGKIVTDKKLDKLISE
                     EQEQVIEVEFDKAVSTELLAMLPNFKSATNTHDLIWELTFISAEDMRSKLFDFANENG
                     LKTLQLNLKSKNLEQIFREKTK"
     gene            complement(1138..1875)
                     /locus_tag="P5335_11015"
     CDS             complement(1138..1875)
                     /locus_tag="P5335_11015"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRZ75454.1"
                     /translation="MKKIFFISVVSFLLFSCEKPGDCIKSTGHMVTKEIPATPFDVIY
                     VYAGIGLVVKEGPEYKVEIRSGENLIDDISAVFQENVLTFRDNTTCNWVRDYGETTVF
                     ITAPNIVEIHSRTEQEIKSDGIITFPILRLISMDLSEGAGTGDFIFQLDNGQLVIENN
                     NVSRFYISGKTQEALLNFYDGNGRIEAQNLEIKKAKIFHRGSNDMHINPIESVEGELF
                     STGNLVLYNSPPMVNVIQHYYGKLIYN"
     gene            complement(1879..2964)
                     /locus_tag="P5335_11020"
     CDS             complement(1879..2964)
                     /locus_tag="P5335_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRZ75455.1"
                     /translation="MRKFYFVLLLFSASLLAQEKTETTALDVHYFTGNVMPHTDDLQH
                     FLNGHPDGVLVSLYKKTYGYEEWEREFGFPDYGAYFIYQDYKNDILGKVYGVGAQYHF
                     YFLNRKVYFKIGEGIAYTTNPHDKETNSKNSAFGSKFVANTNFAINYKTRLFDKVDFQ
                     AGLLFTHFSNGRIKSPNSGVNSYAINVGLSYNFEEPLEKQIDTLPKVKFTEPIKYNVV
                     LRTGMSESPIIASGQKPFFHVGFYADKRLSRKSAIQLGTEIFLTYSYKEFIKYQSIAY
                     PEENVTTDTDFKRGGIFVGYELFINRISLEAQLGFYVYQPYKSDIPVYDRLGMKYYIT
                     KKIYTGLSVKTHIFLAEAMEFVVGVRL"
     gene            complement(3043..>3882)
                     /locus_tag="P5335_11025"
     CDS             complement(3043..>3882)
                     /locus_tag="P5335_11025"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRZ75456.1"
                     /translation="MEFNPPFIDVTYHREEYEFKELPSGLLQKKIVKKRPGTVGICAG
                     IQNKYDVDAIPHILCGGFTKEDTENLLIDLDFLGIDNVVALRGDALKNETYFRPEKEG
                     NEYATELVSQISNLNNGIYLDEDLKNSAKTNFCIGVAGYPEKHMEAPSLDSDIHFLKQ
                     KIKNGADYIITQMFFDNNKFFDFVKKCRAAGITVPIIPGLKPIATKKQLNLIPHRFSI
                     ELPDDLIMAIVKAKDNEDVKQIGIEWCLQQSKELVAAGIPVLHYYSMGKAENIKSIAK
                     EIF"
BASE COUNT         1328 a          676 c          569 g         1309 t
ORIGIN      
        1 tgaatcggga tttatattgt tttgaattaa aaaatttacc ttacataaca caataccaat
       61 cccgactttt tcccaaagaa aaaataaaag aaattagtat aataaaacgt gttgtgtgaa
      121 atcatcttta tgttgtgatt tagttttgct aatataggga aatattctaa caaaaatact
      181 tttctgaaaa aaaatgagtt aaataaaaaa ctatactttt gctgtaaatc tgtaattatg
      241 tctattgaag tcaaaaatat ttctaaaatt tatggagatc aaaaagcatt ggacaacatt
      301 tcattttctg taaaaaatgg agaaattgtt ggctttttag gtccaaatgg tgccggaaaa
      361 tctactttaa tgaagatttt gacaacgtat atcacagctg atgaaggaac tgcttctgta
      421 aacggcttag atgttatttc ctctaaaaaa gaagttcaga gaatagttgg ttatttaccg
      481 gaacacaatc cgttatattt agacttatat gtacgcgaat atttagcctt taatgcggat
      541 gtgtataaaa ttgataaatc tcgaatccaa gaagtgattg aattaaccgg attgactcca
      601 gaaagcacta aaaagatagg tcaattgtca aaaggctacc gtcaaagggt tggattagcc
      661 acggcactat tacataatcc tgaagttttg attttagatg agcctacaac aggtttagac
      721 cctaatcaat tagtagaaat ccgagaactt atcaagaata tcggaaaaga taaaaccgtt
      781 tttctttcca ctcatatcat gcaggaagtg gaagccattt gcgaccgtgt gattattatc
      841 aatcacggaa aaattgtgac ggataaaaaa ctggataaac ttatttcaga agaacaggaa
      901 caagtgattg aagtagaatt tgacaaagct gtttcaacag aattattggc tatgttgcca
      961 aattttaaat cggctacaaa tacacatgat ttaatttggg aattaacctt tatctctgct
     1021 gaagatatgc gttctaaatt atttgatttt gcaaatgaaa atggtttaaa aaccttgcaa
     1081 ttgaatttga aaagcaaaaa cttagagcag attttcagag agaaaacaaa ataattatta
     1141 attatatata agtttaccgt aataatgttg aattacattt accattggtg gtgaattata
     1201 aagcactaaa ttaccggtac tgaataactc tccttcgaca ctttcaattg gattaatatg
     1261 catatcattt gaaccacgat gaaatatttt cgctttttta atttctaaat tttgtgcttc
     1321 aatccgacca tttccatcat aaaaattgag taaggcctct tgtgtctttc cggaaatata
     1381 aaacctcgaa acattattat tctcgatcac taattgtccg ttatcaagtt gaaagataaa
     1441 atcacctgtt ccggcacctt cacttaaatc catggaaata agccgtaaaa ttgggaaggt
     1501 aattattcca tccgatttaa tttcctgttc ggttctcgag tgaatttcaa caatatttgg
     1561 agcagttata aaaacagtgg tttccccata atcgcgtacc caattacaag ttgtattatc
     1621 tctaaatgtc aatacattct cttgaaaaac agctgaaata tcatcaatca aattttcacc
     1681 tgaacgaatt tcaactttat attcgggtcc ttctttaact actaaaccaa tgccggcata
     1741 aacataaatc acatcaaatg gcgttgccgg aatttccttt gtgaccatgt gaccggttga
     1801 ttttatacaa tcgccgggtt tttcacaact gaaaagtaaa aatgaaacaa cactgataaa
     1861 aaatatcttt ttcataaatt ataatcttac accaacaaca aattccatcg cttctgctaa
     1921 aaaaatatgt gtttttaccg ataatcctgt atatattttt ttagttatat agtatttcat
     1981 tcctagccga tcataaacag gaatgtccga tttataaggt tgataaacat aaaagcccaa
     2041 ttgtgcttcg agcgaaattc gattaataaa caattcatat ccaacaaaaa tacctcctct
     2101 tttaaaatct gtatcggttg ttacattctc ttcaggataa gcaattgatt ggtattttat
     2161 aaattcttta tacgaatatg tcaagaaaat ttctgtccct agttgaatag cactttttct
     2221 acttaagcgt ttgtcagcat aaaaaccaac gtgaaaaaat ggcttttgac cactggcaat
     2281 tattgggctt tcactcatac ccgttcgtaa aacaacatta tatttaattg gttcagtaaa
     2341 tttaactttt ggaagggtat caatttgttt ttctaatggt tcctcaaaat tataagaaag
     2401 gccaacatta attgcatatg aattgacccc actattagga gacttaattc ttccatttga
     2461 aaaatgagta aatagtaatc cggcctgaaa atcaacctta tcaaaaagac gtgttttgta
     2521 atttatagca aaattagtat tggctacaaa tttagaccca aatgctgaat ttttactatt
     2581 ggtttcttta tcgtgagggt tggttgtata tgcaattccc tcccctattt tgaaatatac
     2641 ttttctattc aaaaaataga aatgatattg agctccaaca ccataaactt tacctaaaat
     2701 atcattttta tagtcctgat aaataaaata agctccataa tccggaaatc caaattctct
     2761 ttcccattct tcgtagccat atgttttttt gtacaaacta actagaacac catccggatg
     2821 accatttaga aagtgctgta aatcatcggt atgaggcatc acattaccgg taaaataatg
     2881 cacatccaat gccgtagtct ctgttttttc ctgagctaaa agagatgccg aaaaaagcag
     2941 caatacaaaa taaaatttcc tcattctaaa agtaattccg acaaatataa cagaatatat
     3001 ttggttcgga aatttaaaaa cgttctaatg ctgagttatg aattaaaaaa tttcttttgc
     3061 gatggatttg atattttctg ctttccccat cgaataataa tgcaataccg gaattcctgc
     3121 cgccactaac tctttactct gttgcaaaca ccattcaata ccaatttgtt taacatcctc
     3181 attatctttg gcttttacaa tagccattat taaatcgtcc ggcaattcaa tactaaatcg
     3241 atgcggaatt aaattcaatt gttttttggt agcaatcggt tttaaacccg gaataatagg
     3301 caccgtaata ccggctgctc tgcatttttt aacaaaatcg aagaatttgt tgttgtcaaa
     3361 aaacatctgt gtaatgatat aatctgctcc atttttgatt ttttgtttca aaaagtgaat
     3421 atcactgtct aaacttggtg cctccatatg tttttcagga taacctgcca ctcctataca
     3481 aaaattagtt ttagctgaat tttttaaatc ttcatccaag taaattccat tattcaaatt
     3541 actgatttgt gacaccaatt ctgttgcata ttcattccct tctttttcag gtctaaagta
     3601 agtttcattt ttcaacgcat caccacgaag ggctactaca ttatcgattc ccagaaaatc
     3661 taaatcgatt aataaatttt ccgtgtcttc tttggtaaaa ccaccacaca aaatatgcgg
     3721 aatggcatct acatcatatt tattttgaat tccggcacaa attccaaccg ttccggggcg
     3781 tttttttaca attttctttt gtaataatcc gcttggtaat tccttgaatt catattcctc
     3841 ccgatgatag gttacatcaa taaaaggtgg attaaattcc at
//
LOCUS       DAOTCW010000187         3838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_18872_length_3838_cov_31.251123, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000187 DAOTCW010000000
VERSION     DAOTCW010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18872_length_3838_cov_31.251123"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1506)
                     /locus_tag="P5335_11030"
     CDS             complement(163..1506)
                     /locus_tag="P5335_11030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRZ75457.1"
                     /translation="MKKIFKFLGIAILLFIIYIGITTYPKLDIVSGFSAKSVASGHFI
                     DGRSLEIIEKGDNDIDNVRLATNEINEKEKVVYSSAFGMKKRKAIYREGLGATLINDD
                     FDETKPYLIPKRTKIETNLPFPYGNLEPKDSLLPEVNYEKLNLAVANAFDQKGEKIKR
                     TRALLVIYKDKIIAEKYEEGFSKNTKFLGWSMTKSITATYFGVLQYQGKIDINKPTPL
                     TEWANDSRSKITLHNLIQMNSGLEWNEDYGNISDVTKMLFLADDMAKVQLEKPAAFEP
                     NKHWNYSSGTTNLLSGILRRQFQNHQDYLDFWYSSLIDKIGMHSMLIEPDMAGNFVGS
                     SYGWATARDWAKFGLLYLHEGNWNGEQIFDKSWAKYVATPTPTSKGEYGGHFWLNAGG
                     KYPDVPKDMYFCGGFQGQKVIIIPSKELVIVRFGLTEDEDFDFNGMMREIITSIK"
     gene            1661..2875
                     /locus_tag="P5335_11035"
     CDS             1661..2875
                     /locus_tag="P5335_11035"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRZ75458.1"
                     /translation="MLDIQKIRADFPILSQKVNGKPLVYFDNAATSHKPIQVIEAEVN
                     YYKEINANIHRGVHTLSQLATDAYEASRSKIQKHINAKSRHEVLFTSGTTHGINLVAN
                     GFASFLKQGDEVMVSALEHHSNIVPWQMLCDKTGAKLAVIPMNESGELILSEYERLLS
                     TKTKIVTVNHISNALGTINPIKWMIEKAHEVGAAVLIDGAQAVPHLKPDVQGLDCDFY
                     VFSGHKICGPTGTGILYGKEVWLNKLPPYQGGGEMIATVTFEKTTYAGLPHKFEAGTP
                     NIAGGIVLGTAIDYLNEIGFDNIEAYEHELLEYGTSRLLEIEGLKIYGTSTQKTSVIS
                     FNVGDIHPYDIGTIIDKLGIAVRTGHHCAQPIMDFYKIPGTLRASFAFYNTKEEIDAL
                     VEAVKKAQMMLS"
     gene            2893..3318
                     /locus_tag="P5335_11040"
     CDS             2893..3318
                     /locus_tag="P5335_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75459.1"
                     /translation="MKTIITIFLTVFLAKSCANENGDIDKAVVEYEATSRGFYKNIKI
                     EDKKLFVINNRNEKPVEVSLSKKEWNELVDAFKEINLEEMKNLKAPTDKRLYDGAAHA
                     NITIILDGKTYTTDGFDHGYPPSQIENFISKLIFLTIKE"
     gene            3321..3740
                     /locus_tag="P5335_11045"
     CDS             3321..3740
                     /locus_tag="P5335_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRZ75460.1"
                     /translation="MTIKEIQNEIVDEFSMFDDWMQRYEYIIELGKALPLIDEKFKTK
                     ENIIKGCQSKVWLQGEEKEGIIVFTADSDAILTKGIIAILIRVFSNQKPAAILEANTD
                     FIDEIGLKEHLSPTRANGLVSMIKQIKMYALAFEAKH"
BASE COUNT         1338 a          653 c          672 g         1175 t
ORIGIN      
        1 gcatcacgaa gattattgcg atgaaattgg gtgtaaattt ggggttcgat ttgtagaaat
       61 taaccacaat aaaaaataag atttacacta aggacgtaaa gtttacataa ttaactttgc
      121 gtcctttgcg atttttactt tgcgttcttt gcggttaaat tatcacttaa tagatgtaat
      181 aatctccctc atcatcccat taaaatcaaa atcctcatct tccgttaaac caaaacgaac
      241 aatcaccaat tctttggatg gaatgatgat tactttttgt ccttgaaaac cgccacagaa
      301 atacatgtct ttcggtacat caggatattt tccgccggcg ttcaaccaaa aatgaccacc
      361 atattctccc ttggaagtgg gtgtgggagt tgcgacgtat ttcgcccaac ttttatcaaa
      421 aatctgctcg ccattccaat tgccttcatg caaataaagc aggccaaatt tagcccaatc
      481 acgagccgtt gcccagccat aagaagaacc cacaaaattt ccggccatat caggttcaat
      541 caacatcgaa tgcattccga ttttgtcaat tagtgacgaa taccaaaaat ccaaataatc
      601 ctgatgattt tgaaattgtc gtcgcaaaat tcccgaaagc aaattggtcg ttccggaaga
      661 ataattccaa tgtttgttag gttcaaatgc agcaggtttt tctaattgta ctttcgccat
      721 atcatcggct aaaaaaagca ttttcgttac atctgagata ttcccataat cttcattcca
      781 ttccaaaccg gaattcattt gaatcaaatt atgcaacgta attttcgaac gtgaatcatt
      841 tgcccattca gttaatggag taggtttatt aatgtctatt ttcccttggt actgcaaaac
      901 tccaaagtaa gtcgccgtaa tacttttggt cattgaccaa cctaaaaatt ttgtgttttt
      961 agaaaaaccc tcttcgtatt tttcagcaat aattttgtct ttataaatca ctaaaagagc
     1021 tcttgttcgc ttgatttttt cgcctttttg atcaaaagca ttagcaacag ccagatttag
     1081 tttttcataa tttacctccg gcaaaagtga gtcttttggc tcaagatttc catatggaaa
     1141 aggtaaatta gtttcaattt tagttcgttt tggaatcaaa taaggttttg tttcatcaaa
     1201 atcatcgtta atcaaagtag ctcctaaacc ttcacggtaa atggcttttc gctttttcat
     1261 cccaaaagcg gaagaataaa ccactttttc cttttcatta atttcatttg tggccaagcg
     1321 aacattatcg atatcattat cgcctttttc aatgatttcc aacgaacgcc catcaataaa
     1381 atggccggaa gcaacacttt ttgccgaaaa accggaaaca atatccaatt ttggataggt
     1441 tgttatgcca atgtaaataa taaaaagtaa aatggcgata ccaagaaatt tgaaaatttt
     1501 tttcatttga aatttattta atgcaatgta taaaattatc tcaaatgaaa actaaaactt
     1561 gagattttca cttgacacaa ttcataaaaa ttcgtgaatt ggtggtaaat aaggaatcct
     1621 ttttactact tttgtattta gaatttttct aaataaagaa atgttagaca tccaaaaaat
     1681 aagagccgat tttccgatac tatctcaaaa agtaaatgga aagccactcg tttatttcga
     1741 taatgcggct acttcgcaca agccaattca agtcattgaa gctgaagtga attattacaa
     1801 agaaattaat gccaacattc accgaggtgt tcacacgttg agtcagttgg caaccgatgc
     1861 ctatgaagct tcacgatcaa aaattcaaaa acatattaat gccaaatcta gacacgaagt
     1921 actttttact tccggaacta cacacggaat taatttagtt gcaaatggat ttgcttcttt
     1981 tttaaaacaa ggtgatgagg tgatggtttc cgctttagaa caccacagca acattgttcc
     2041 gtggcaaatg ttatgtgata aaaccggagc caaattagcc gttattccta tgaacgaatc
     2101 aggagaattg attctttcag aatatgaacg tttactttca accaaaacga aaattgtaac
     2161 agtaaatcat atttccaatg cgttgggaac catcaatccg attaaatgga tgattgaaaa
     2221 agcccacgaa gttggtgcag ccgttttaat agatggagca caagcggtgc cacatttaaa
     2281 acctgatgtt cagggattag attgtgattt ttatgttttt tccggacaca aaatttgtgg
     2341 accaaccgga acaggaattt tatacggaaa agaagtttgg ttaaataaat tacctcctta
     2401 tcaaggtggt ggcgaaatga ttgcaacagt tacgtttgaa aaaacaacgt atgccggact
     2461 gccacataaa tttgaagccg gaacacctaa catcgcagga ggaattgttc ttggaaccgc
     2521 gattgattat ctcaatgaaa tcggttttga taatattgaa gcgtatgaac acgaattgtt
     2581 agaatacgga accagccgat tattagaaat tgaaggttta aaaatatacg gtacgtcaac
     2641 acaaaaaact tccgtaattt catttaatgt tggcgatatt catccgtatg acattggtac
     2701 gataattgac aaattaggaa tagctgttcg caccggacac cattgtgctc aaccgattat
     2761 ggatttttat aaaattccgg gaacattaag ggcttcgttt gctttttata atacaaaaga
     2821 agaaatcgac gcattagtgg aagcggtaaa aaaagctcaa atgatgttga gttaacctaa
     2881 aaacaaaata aaatgaaaac aattattact atttttttaa ccgttttttt agccaaaagc
     2941 tgtgctaatg aaaatggaga tatcgacaaa gcggttgttg aatatgaagc aacgtcaaga
     3001 ggtttttata aaaatataaa aattgaagac aaaaagcttt ttgttataaa taacagaaat
     3061 gaaaagccgg ttgaagtttc gctttcaaaa aaggaatgga atgaattagt ggatgctttc
     3121 aaagagatta atttggaaga aatgaaaaac ctaaaggctc caactgataa aagattatat
     3181 gatggagcag ctcatgcaaa tattacaata atattagatg ggaaaaccta taccacagat
     3241 ggttttgacc acggttatcc accaagtcaa attgagaact tcatcagtaa attaattttt
     3301 ttaacgatca aggaataagt atgaccatca aagaaataca aaatgaaatt gttgacgaat
     3361 tctcaatgtt cgacgattgg atgcagcgtt atgagtacat cattgaactc ggaaaggcgt
     3421 tgcccttgat tgatgaaaag ttcaaaacga aagaaaatat tatcaaaggt tgtcagtcga
     3481 aagtttggct acagggcgaa gaaaaagagg gcataattgt ttttacagcc gatagtgatg
     3541 ctatcttaac aaaaggtatt attgctattt taattcgggt tttttctaat caaaaaccag
     3601 cagcaatttt agaagcaaac acagatttta ttgatgaaat cggtttaaaa gagcatttat
     3661 cgccgactcg tgctaatgga ttggtgtcaa tgattaaaca aattaaaatg tatgctttag
     3721 cttttgaagc gaagcattaa aaataataat gttcagatga aacttactga actaaattca
     3781 gtgtaaaatg gaagaattta aagacgacat aaacttaggt gaaaacgtag taaaggta
//
LOCUS       DAOTCW010000188         3828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_18943_length_3828_cov_36.702359, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000188 DAOTCW010000000
VERSION     DAOTCW010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18943_length_3828_cov_36.702359"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="P5335_11050"
     CDS             complement(<1..659)
                     /locus_tag="P5335_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRZ75461.1"
                     /translation="MKAFDTKLFKRILFYTKPYQWRFNGVVIWAVSLSIFAALRPYLL
                     KETVDNYIKTNDGQGLLFYIVLMGIILLMEVLSQFYFVYWANWLGQDIVKDIRIKLFD
                     HMLRFKMKYYDNSPVGQLVTRSVSDIEAIARIFSQGLFMIISDLMKMVVVLIFMFFMN
                     WKLTWIVILAMPILLYATRIFQLKMKVAFEEVRTQVANLNTFVQERVTGMKIVQLFTR
                     E"
     gene            complement(656..1396)
                     /gene="truA"
                     /locus_tag="P5335_11055"
     CDS             complement(656..1396)
                     /gene="truA"
                     /locus_tag="P5335_11055"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRZ75462.1"
                     /translation="MRYFIKLAYNGTNYHGWQSQPNASSVQETLEKALSLLLKSKIEI
                     VGAGRTDTGVHAKEMFAHFDYPEPLDAAFWITKLNSYLPKDIVIYTIIPVSDEAHARF
                     DATSRTYQYHINTFKDAFSFEQSWYHHYPLDLAKMNEAALILMEYTDFECFSKANTDV
                     FTFNCKITQAFWQQNGNQVVFTITADRFLRNMVRAIVGTMINIGQEKIAVAHLRKIIE
                     SKNRNEAGFSVPAQGLYLTQIVYPYLHE"
     gene            1567..>1695
                     /locus_tag="P5335_11060"
     CDS             1567..>1695
                     /locus_tag="P5335_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRZ75463.1"
                     /translation="MKKILLLSDTHSFLDEKILKYVRLVDEVWHAGDIGNLTVTDEI"
     assembly_gap    1696..1795
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1796..3375)
                     /locus_tag="P5335_11065"
     CDS             complement(<1796..3375)
                     /locus_tag="P5335_11065"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRZ75464.1"
                     /translation="MNKHFFFFFLIVFNVSIFAQPKIDSVDYYMEKNELIKGLNYAKT
                     KSEYYFQENQFDKFCRISVKKAKIYGGLNDHDKALSTLYKALAVSDKNKLKGNAEILE
                     QIGTRYSIIRDSTKAFKNYYKALNIGLKEKDTASLIHVYHNLFRLNTEKNLDSCYKYM
                     VKKYELDRGSKSISGISFSYNNYFCYYTLKGEPELAKTYLDSCYNFSLKHKLNKSIIS
                     SLSNLGYYYMETEKDFKKALAIYDKLLLDYKKEFSTLDFVDIYLNLVYAYEQQKDYKR
                     ANYYNTLHQEYKEKLYNEKINDKVREIETKYAIDKVENEFNEKSRHLVENENRNKKII
                     FIFVALFGFSLVLFYFFYQNLQLKQRNKIKELDSEIQENIINASIDGQELERKKLAEV
                     LHDNISALLSSASLHLSAFLVGQNNHQPEEILKARSLLKEAHDKVRDLSHELVPPVLA
                     KLGLYHAIQDLCEKNSNSIIYFTFNYYGSNEKRYNEEFEIKVYFIITELINNILKHSK
                     ATIGNITLEERNNQLIIN"
     gene            complement(3368..3661)
                     /locus_tag="P5335_11070"
     CDS             complement(3368..3661)
                     /locus_tag="P5335_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75465.1"
                     /translation="MELLGTYTITRIDEKNYTLVYEIPDNCDFSVADKDGVYAITIEL
                     KPGQTEPSLKYVTETLNCEAFAGIIDVKFIQQDYENLGATYSTRPKVKININE"
     gene            complement(3664..>3828)
                     /locus_tag="P5335_11075"
     CDS             complement(3664..>3828)
                     /locus_tag="P5335_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRZ75466.1"
                     /translation="KLISLEYSGKEIGEELFISANTVETHRKNLIKKLNVKNTIGLVK
                     YAIKNNLIKQ"
BASE COUNT         1195 a          622 c          599 g         1312 t
ORIGIN      
        1 tcttcacggg taaaaagttg aacaattttc attccggtca cgcgttcctg cacaaaagta
       61 ttgaggttgg ctacctgagt tctcacttct tcaaaagcaa ctttcatttt caattgaaaa
      121 atacgggtag catagagtaa aataggcatg gctaaaatca caatccaagt taacttccag
      181 ttcatgaaga acatgaaaat caaaacaaca accatcttca ttaaatcact gataatcatg
      241 aacaatcctt gactgaaaat acgggcaatc gcttcaatat ctgaaacaga ccgtgtaacc
      301 agttggccca cgggagaatt gtcataatat ttcattttaa accgaagcat gtgatcaaaa
      361 agcttaatcc ggatgtcttt tacgatatcc tgacctagcc agttcgccca ataaacaaaa
      421 taaaattgcg ataaaacctc catcaacagg ataattccca tcaacacgat gtaaaacaat
      481 aagccttgtc cgtcgtttgt tttaatgtaa ttatccaccg tttccttcaa caaatagggg
      541 cgaagggcag cgaatattga aagcgataca gcccaaatca ccacgccgtt aaaccgccac
      601 tgatagggtt tagtatagaa caaaatccgt ttgaataatt tagtatcgaa ggctttcatt
      661 cgtgtaaata ggggtaaaca atttgggtta aatataatcc ttgtgccggt acagaaaatc
      721 cggcttcgtt gcgatttttg ctttcaataa tttttctcaa atgtgctacg gctatctttt
      781 cttgtccgat atttatcatc gtgccaacga tggctcgaac catattccgt aaaaaacggt
      841 cagccgtaat ggtaaacact acttgatttc cgttttgttg ccaaaaagct tgtgttattt
      901 tacaattaaa cgtaaacaca tcagtattcg ctttagaaaa acattcaaaa tctgtgtatt
      961 ccattaaaat aagggccgct tcattcattt tagccagatc taaaggataa tgatggtacc
     1021 aactttgttc aaatgaaaaa gcatccttga atgtattgat atgatactga taggttctgc
     1081 tggtggcatc aaaacgggca tgagcttcat cagaaacagg aataatagta tagataacga
     1141 tatcctttgg cagataagag ttcagttttg taatccaaaa agcagcatca agaggttcag
     1201 gatagtcaaa atgagcaaac atctctttcg catgaacacc cgtatcagtt cttccggcac
     1261 caacaatttc aattttgctt tttaacaata gcgaaagtgc cttttccaat gtttcctgaa
     1321 cagaagaagc attaggttgc gattgccaac cgtggtaatt tgtgccgttg tatgctaatt
     1381 tgataaaata cctcataatt ccggtagcaa agataattga attgaatttg tgacaaattt
     1441 acaagcggta taattttaag ttaaacttgc taccacaagt aaaaatagag ataagatttt
     1501 atagccaaaa gggaaaggat tacttttcac tttgtatctt cgttccatta ttgcaaaaat
     1561 acaaaattga aaaaaattct gcttctatca gacacacaca gtttccttga tgaaaagata
     1621 ttaaagtatg ttcgtttagt cgatgaggtt tggcatgccg gtgacattgg caatttaacc
     1681 gttacagatg aaatcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatatt
     1801 aataataagt tggttgttcc gttcttctaa agtaatattt cctatggtcg ctttgctgtg
     1861 ttttaaaata ttattaatca gctcagtgat gataaaataa actttaattt caaactcttc
     1921 gttatagcgt ttttcattag agccataata gttaaaggtg aaatagataa tagaattgga
     1981 attcttttca cacaaatcct gaatagcatg gtataatccg agcttggcta atacgggcgg
     2041 tactaattca tgggacaaat cgcgtacttt gtcatgagct tctttaagta aagacctagc
     2101 ttttagaatt tcttccggtt ggtgattatt ctgaccaact aaaaatgccg ataagtgcaa
     2161 actggcagag gatagcaacg cactaatgtt gtcgtgaagc acttcagcta attttttgcg
     2221 ttccagttct tgtccgtcga tggatgcatt gataatattt tcttgaattt cgctatcgag
     2281 ttctttaatt ttattccttt gcttgagttg taagttttgg tagaaaaagt aaaatagcac
     2341 caaactaaat ccaaacagag ccacgaaaat gaaaattatt tttttgtttc gattttcgtt
     2401 ttcaactaaa tgtcttgatt tttcattgaa ttcattttca actttgtcaa ttgcatattt
     2461 agtttcaatt tcccgaactt tatcgtttat tttttcattg tataattttt ctttatattc
     2521 ttggtgcaaa gtattgtaat aattggctct tttataatct ttttgctgtt cataggcata
     2581 cactaaattt agatatatat ctacaaaatc aagagtgctg aattcttttt tgtaatcaag
     2641 taataattta tcatagattg ctaaggcttt tttaaagtct ttttcggttt ccatgtaata
     2701 ataacctaaa ttgctcaatg aagaaataat agatttattc aatttatgtt tcaaggagaa
     2761 gttataacaa ctatctaaat aagtttttgc aagttcaggt tcccctttga gggtataata
     2821 gcagaaataa ttattataac taaatgaaat tcctgaaata gattttgaac ctctatctaa
     2881 ttcatatttc ttgaccatat acttatagca actgtctaaa tttttttccg tattcaatcg
     2941 aaataaatta tgataaacat gaattaagct ggcagtgtct ttttctttta agcctatgtt
     3001 taatgctttg tagtaatttt taaaagcttt agtggaatct ctgattattg aataacgggt
     3061 tcctatttgt tctaaaattt cagcattacc ttttagttta tttttatcag aaactgctaa
     3121 tgctttataa agcgtactca aagctttatc gtggtcgttt aatccaccat aaattttggc
     3181 tttcttgacc gatattctac agaatttatc aaattgattt tcttgaaaat aatattctga
     3241 tttagtttta gcataattta atcctttaat taactcgttt ttttccatat aataatctac
     3301 cgaatcaatt ttaggctgag caaaaattga cacattgaat actattaaaa agaaaaaaaa
     3361 gaagtgttta ttcattgata tttattttaa ctttaggtct tgttgaataa gtagccccta
     3421 aattttcata atcctgctga ataaatttaa catcaattat gccggcaaaa gcctcacaat
     3481 taagtgtttc cgttacatat tttaaagagg gttctgtttg tccgggtttt aattcaatag
     3541 tgatagcata aactccgtct ttgtccgcta cagaaaagtc gcaattgtcc ggaatttcat
     3601 aaactaaagt gtagtttttt tcatcaattc ttgtaatggt gtaagtgcct aaaagttcca
     3661 taattattgt ttgattaagt tgtttttaat tgcatatttt actaatccaa tagtgttttt
     3721 tacatttagt tttttgatta aattcttgcg gtgggtttct acggtattgg cactgataaa
     3781 aagttcttcg ccaatttctt tcccactgta ttccaaggag attaactt
//
LOCUS       DAOTCW010000189         3827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_18953_length_3827_cov_36.389979, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000189 DAOTCW010000000
VERSION     DAOTCW010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18953_length_3827_cov_36.389979"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="P5335_11080"
     CDS             complement(<1..869)
                     /locus_tag="P5335_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRZ75467.1"
                     /translation="MSYYKIHDLENYFKMYKKSVREPRKFWDRIADENFTWYQKWDKV
                     FEFDMQEAKFKWFLDAKVNITKNCIDRHLAKRGEKAAIIFEPNDPKEEAQTITYNELY
                     ARVSKMANVLREQGIKKGDRVCIYLPMIPELAVSVLACARIGAIHSVVFAGFSASAVA
                     TRINDSECKMVITSDGGFRGNKTIDLKGIIDEALEKCPSVEKVLVVKRTNEKVIMKEG
                     RDLWLQPLLDEAIANNVAEIMDAEDPLFILYTSGSTGKPKGMLHTTAGYMVYTAYTFK
                     NVFNYEENDIYWCT"
     gene            complement(950..2845)
                     /locus_tag="P5335_11085"
     CDS             complement(950..2845)
                     /locus_tag="P5335_11085"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRZ75468.1"
                     /translation="MNYKDFYQKSLENPELFWKEQADQIEWYAKPTNILSKDKNDYPL
                     WYADGELNMCYLALDKHIQDGFGDNIAMIYDSPVTQTIKKYTYQEVKTEVAKLAGGLL
                     SLGLKKGETAVIYMPMIPQAAFAMLACARIGVTHSVVFGGFAPHELAIRINDCKPRVI
                     ITASSGIEVDRLIAYKPLVDEAIELADHKPKKVVVLNRKLGARVPFKKYDVDYDALVY
                     GSEEVACVPVNSTHPLYVLYTSGTTGKPKGIVRDTGGYATALKFSMQYIYGVKEDEVF
                     WAASDVGWVVGHSYIVYGPLINRNTTIIFEGKPIKTPDASTFWRVIAEHNVSVMFTAP
                     TAIRAIKKEDPNGDFINEYDLSCLRTQFLAGERCDVATLEWYNKHILVPAIDHWWQTE
                     SGWPMISNMMGVEFLPIKPGSAGKAVTGYNIQILNDNGLEVGPNEEGFVVIKLPLPPG
                     TMLDLWNDSPRFKSGYLKTFPGYYFSGDGGFKDADGYIFITGRVDDVINVAGHRLSTA
                     EMEEIVASHHSVAECAVIGINDELRGQIPLAMVVTKTGETIEHFQLEYEIVHLIRDQI
                     GAVACLRNVMIVDRLPKTRSGKILRKLLRSIVDKENYQIPSTIDDEAIIGEIEEVIEK
                     YHESKAK"
     gene            complement(2910..3272)
                     /locus_tag="P5335_11090"
     CDS             complement(2910..3272)
                     /locus_tag="P5335_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRZ75469.1"
                     /translation="MKKILLVDDEPNIIMSLEYTFKKNNFQVFIARDGQEALEILKTE
                     QPDLIILDIMMPNVDGYATIEAVRKDEKLSNCKVIFLSAKNKESDIEKGMRLGANAYM
                     TKPFSIKKLIEQVNELLE"
     gene            complement(3288..>3827)
                     /locus_tag="P5335_11095"
     CDS             complement(3288..>3827)
                     /locus_tag="P5335_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRZ75470.1"
                     /translation="TESDRLNRLIDKILDLEKFETGKQKIYLSKGNIIKTIANSLSPL
                     QQLIANKNIHLDFNENQSAELLNFDEERIVQVITNLVSNAIKFCDEHNGLILISVENK
                     EEEAIINVFNNGKGVNPDDLETIFDKFYQSSNQNIKKPIGSGLGLAICKQIVEAHKGK
                     IWATNVDKGVTFSFTIPKI"
BASE COUNT         1137 a          727 c          595 g         1368 t
ORIGIN      
        1 gtacaccaat aaatatcatt ttcttcgtaa ttgaatacgt ttttaaaagt ataagctgta
       61 taaaccatgt atcctgctgt ggtatgcaac attcctttcg gtttacctgt ggaacctgaa
      121 gtatacaaga taaataaggg gtcttctgca tccataattt cagccacatt attagctatg
      181 gcttcatcta ataacggttg caaccataaa tcacgacctt ctttcatgat aaccttctca
      241 ttggtgcgtt taacaaccaa tactttttca acagatggac atttctctaa agcttcgtcg
      301 attattcctt ttaaatcaat ggttttattt cccctgaaac caccatcgga agtaataacc
      361 attttacatt cgctgtcatt aattcgagta gcgactgccg atgccgaaaa tccggcaaat
      421 actaccgaat gaatggctcc tattctggca caagctaaaa ctgaaactgc caattcggga
      481 atcattggta aataaataca aactcgatca ccttttttga ttccttgttc gcgtaaaaca
      541 tttgccattt ttgaaactcg ggcatacaat tcattgtagg ttattgtttg tgcttcttct
      601 ttcggatcat ttggttcaaa gataatggct gctttttcac ctcttttagc taggtgccgg
      661 tcaatacagt tttttgtaat attgacttta gcatctaaaa accatttgaa tttagcttcc
      721 tgcatatcaa attcaaaaac tttgtcccat ttttgatacc aagtaaagtt ttcatcggcg
      781 atgcgatccc aaaattttcg gggttcacga accgattttt tatacatttt aaaatagttt
      841 tctaagtcgt gtattttata ataactcatt gtttttgtct ttttagaatt gtaaaaataa
      901 gaaatattcg ttttaaatgt tgtgtataat tgctaaaata ctatagctat tactttgctt
      961 tgctttcatg atatttttcg ataacttctt caatttctcc tataattgct tcatcatcaa
     1021 tagttgatgg aatttgataa ttttctttat ccactatact acgtagtaat tttcgaagta
     1081 ttttaccgga acgggttttg ggtaatcgat caacaatcat gacatttcgc aagcaggcaa
     1141 cagcaccaat ctgatctctg attaagtgaa caatttcata ttccaattga aagtgttcaa
     1201 tcgtttctcc tgttttggta accaccatcg ctaagggaat ttgaccgcgt aattcgtcat
     1261 ttattccaat tacggcacat tcagcaacgg aatgatgtga agctactatt tcttccattt
     1321 cagcggttga caaacggtgt ccggctacat tgataacatc atccactcta ccggtaatga
     1381 agatgtatcc atcagcatct ttaaaaccac catcaccgga aaaataatag ccgggaaatg
     1441 tttttaaata acctgattta aatcgagggg aatcattcca taaatcaagc atggtgcctg
     1501 gaggtaatgg taattttata actacaaatc cttcttcatt tggtcctact tctaagccat
     1561 tgtcgtttaa aatttgaatg ttgtaacctg tcacagcttt acccgcagaa cccggtttaa
     1621 ttggcaagaa ttcaacgccc atcatattgg aaatcatagg ccagcctgat tctgtttgcc
     1681 accaatggtc aattgcagga actagaatat gcttgttata ccattctaac gttgcaacgt
     1741 cacaacgttc accggctaaa aattgtgttc ttaaacagga taaatcatat tcattgataa
     1801 aatcaccatt tggatcttct tttttaattg ctctaattgc tgttggtgcg gtaaacatca
     1861 cactcacatt gtgctcagct atcacacgcc aaaacgtaga ggcatccggt gttttaattg
     1921 gtttaccttc aaaaattatg gttgtatttc gattaatcaa aggtccataa acgatataac
     1981 tatggcctac aacccaacca acatctgaag ctgcccaaaa aacttcatct tctttaacac
     2041 cataaatgta ttgcatagaa aatttaagag cagtggcata acctcccgta tctctgacaa
     2101 ttccttttgg ttttcctgtt gttccggaag tatataaaac atataatgga tgggtcgaat
     2161 ttacaggaac acaagctact tcttcagaac cataaaccaa agcatcataa tctacatcat
     2221 actttttaaa aggaactctt gctcctaatt ttctatttaa tacgaccact tttttgggtt
     2281 tgtgatcggc taattcgatt gcttcatcca ctaatggttt ataagcaatt aaacgatcta
     2341 cttcaattcc ggaagaggca gtaataatta ctctgggttt acaatcatta attctgatag
     2401 ctaattcatg tggagcaaat ccaccaaaca caactgaatg tgtcacacca attctagcac
     2461 aagccaacat ggcaaaagcg gcttgtggaa tcatcggcat ataaataaca gctgtttcac
     2521 cttttttcaa acctagcgat agtagaccac cggctagttt tgccacttct gtttttactt
     2581 cctgatacgt atattttttg atggtttgcg ttaccggtga atcataaatc atcgcaatgt
     2641 tgtcaccaaa accgtcttgt atatgtttgt ctaaggctaa ataacacatg tttaattcac
     2701 catcggcata ccacaaggga taatcatttt tgtcttttga aagaatgttg gttggtttgg
     2761 cataccattc tatttggtct gcttgttctt tccagaataa ttccggattt tcaaggcttt
     2821 tttgataaaa atctttataa ttcatttttt ttaagttgct ggtttattgt atagttgagt
     2881 tatctgtgtt tgtcttttga ctaagaatgt tactctaaca attcattcac ttgttcgatt
     2941 aattttttta tggaaaaagg ttttgtcata taggcatttg ctcctaaacg catccctttt
     3001 tcgatgtcgc tttctttatt ttttgcggag agaaaaatca ctttgcaatt tgatagtttt
     3061 tcatcttttc ttaccgcttc aatagtggca tacccatcaa cattaggcat cattatatct
     3121 agtataatca aatcaggctg ttctgtttta agaatttcta aagcttcctg accgtcacga
     3181 gcaataaata cttgaaaatt attcttctta aaagtatatt ccaaggacat aatgatgttg
     3241 ggttcgtcat caactaaaag tatctttttc ataggcagtt ggtttaatta tatttttgga
     3301 atagtaaatg agaaagtgac tcctttatca acattagttg cccaaatctt acctttatgg
     3361 gcttctacaa tttgtttaca aatagccaat cctagtccgc ttccaattgg ttttttaata
     3421 ttctgattac tggattgata aaacttatca aaaatggttt ctaaatcatc cggattaaca
     3481 cctttaccgt tattgaacac atttatgatg gcttcttctt ctttattttc aacagaaatt
     3541 agaattaaac cattatgctc atcacaaaat ttaatggcgt tcgaaaccaa attggtaatt
     3601 acttgaacta ttctttcttc atcaaaattt aataattcag ccgattgatt ttcattaaag
     3661 tccaagtgaa tatttttatt ggcaatcagt tgttgtaaag gagataagga attagcaata
     3721 gtttttatta tatttccttt tgataggtat attttctgtt taccggtttc aaatttttct
     3781 aaatctaaaa tcttatcgat gaggcgattt aatcgatcgg attcggt
//
LOCUS       DAOTCW010000190         3822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_18993_length_3822_cov_40.903637, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000190 DAOTCW010000000
VERSION     DAOTCW010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18993_length_3822_cov_40.903637"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1967)
                     /locus_tag="P5335_11100"
     CDS             complement(<1..1967)
                     /locus_tag="P5335_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRZ75471.1"
                     /translation="MDDNFSPRVKDVITYSKEEALRLGHDFIGTEHLMLGILRDGNGK
                     AITILNNLDIDLDYLRKKVEILSPPNPNAEAGNEKKNLHLTRQAERALKTTFLEAKVY
                     QSSSISTAHLLLCILRNENDPTTKLLNKLKIDYDSAKEQYLNMIPNNNEDEYTDSFPK
                     NESFNDDSGQDDSLKEGSFNNPASKTNKKSKTPVLDNFGRDLTEMAEEGKLDPVVGRE
                     KEIERVSQILSRRKKNNPLLIGEPGVGKSAIAEGLALRIIQKKVSRILFNKRVVTLDL
                     ASLVAGTKYRGQFEERMKAVMNELEKNDDIILFIDEIHTIVGAGGATGSLDASNMFKP
                     ALARGEIQCIGATTLDEYRQYIEKDGALERRFQKVIVEPTSIEETITILNNIKGKYED
                     HHSVTYTPEAIDACVKLTSRYMSDRFLPDKAIDALDEAGSRVHITNIEVPKQILELEK
                     QLEDVRELKNTVVKKQKYEEAAKLRDDEKRIEKELAIAQEQWEVDSKNNRITVTEDNV
                     ADVVSMMTGIPVNRIAQTESNKLAHLPELIQNKVIGQNEAVMKVARSIQRNRAGLKDP
                     NRPIGSFIFLGQTGVGKTQLAKVLAKELFDSEDALVRIDMSEYMEKFAISRLVGAPPG
                     YVGYEEGGQLTEKVRRKPYCVVLLDEIEKAHP"
     gene            2225..>3822
                     /gene="gyrA"
                     /locus_tag="P5335_11105"
     CDS             2225..>3822
                     /gene="gyrA"
                     /locus_tag="P5335_11105"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HRZ75472.1"
                     /translation="MSEGEKLIPINIEDEMKSAYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLYGMYDLGVFSNRAHKKSARIVGEVLGKYHPHGDTSVYDAMVRMAQEWSLRYLLV
                     DGQGNFGSVDGDSPAAMRYTEARMQKISEEIMADIEKETVDFQLNFDDTLYEPKVMPT
                     KVPNLLINGASGIAVGMATNMPPHNLTEVVNGTLAYIDNTEIEIDELMDYIKAPDFPT
                     GGIIYGYDGVREAFKTGRGRIVMRAKTGFEESNGREAIIVTEIPYQVNKADMIKKTAD
                     LVNDKKIEGISNIRDESDRNGMRIVYELKRDAVPNVVLNTLYKYTQLQSSFSVNNIAL
                     VNGRPQMLNLKELIHYFVEHRHDVVVRRAKFDLRKAEERAHILEGLIIASDNIDEVIA
                     LIRSSKDGDEARTKLIERFSLSEIQARAIVEMRLRQLTGLEQDKLRAEYEDIMKLITH
                     LRELLANKDLRMQVIKDELVEIRDKYGDERRSVIEYSGGDVSIEDLIADENVVITISH
                     AGYIKRTNLSEYKTQNRGGVGQKSA"
BASE COUNT         1182 a          647 c          703 g         1290 t
ORIGIN      
        1 ggatgagctt tttcaatttc gtctaataaa actacacaat agggtttgcg acgaactttt
       61 tcggttagtt gaccgccttc ttcgtagcca acatatcccg ggggagcacc aaccaatctg
      121 gaaattgcaa atttttccat gtattcactc atgtcaattc taaccaaggc atcttctgaa
      181 tcaaaaagtt ctttagccaa aacttttgct aattgtgttt ttccaacacc ggtttgacct
      241 aagaaaatga acgaaccaat aggtcggtta gggtctttta atcccgcacg attacgttgg
      301 attgaccgag ctactttcat aacagcttca ttctgaccaa tgactttatt ttgaattaat
      361 tcaggtaaat gggctagttt attactttct gtttgagcaa ttcggttaac aggaattccg
      421 gtcatcatgg aaacaacatc ggcaacatta tcttccgtaa ccgttattct gttgtttttt
      481 gagtcaactt cccattgttc ttgagcgatt gctaattctt tttcgatgcg tttttcgtca
      541 tcgcgaagtt tcgccgcttc ctcatatttt tgctttttaa cgacagtatt ttttaattcc
      601 cgaacatcct ctaattgttt ttccaattct aaaatttgtt ttggaacttc aatattagta
      661 atgtgtacac gagaaccggc ttcatctaat gcatcaattg ctttgtccgg taaaaatcgg
      721 tcagacatgt aacgactggt taattttaca caggcatcaa tggcttccgg tgtgtaagtt
      781 acgctgtgat gatcttcata tttacctttg atattattta aaatggtaat ggtttcctca
      841 attgatgttg gttcaacaat cactttttga aaacgacgtt ctaaagcacc atctttttca
      901 atatactgac ggtattcatc taaagttgta gcaccaatac attgtatttc accccttgcc
      961 aaagctggtt tgaacatatt tgaggcgtct aacgaacctg ttgctccacc ggcaccaaca
     1021 atcgtatgaa tctcatctat aaacagaatg atatcgtcgt ttttttctaa ttcattcatg
     1081 acggctttca tacgttcttc aaactgaccg cggtattttg ttccggcaac taagcttgct
     1141 aaatcaagtg taacaactct tttgttaaac aaaatacgag aaactttctt ttgaataatt
     1201 cgtaaggcta aaccttcagc aatggccgat ttacctacac caggttcacc aattaaaagc
     1261 ggattatttt tctttcttct gcttaaaatt tgtgaaacac gttcgatttc tttttcacgt
     1321 ccaactacag gatctagttt tccttcttcg gccatttctg ttaaatcacg accaaaatta
     1381 tctaacacag gggttttaga ttttttgtta gttttacttg ccggattatt aaaactacct
     1441 tcttttaaac tgtcatcttg tccggaatca tcattaaacg attcgttttt tgggaagctg
     1501 tcagtatact cgtcttcgtt gttatttggt atcatattca gatattgttc tttagctgag
     1561 tcgtagtcga ttttcagctt gtttaataat ttagttgtag gatcattctc gttccttaaa
     1621 atacaaagca gaagatgtgc cgtactaata gacgaacttt ggtaaacttt tgcttctaaa
     1681 aaagttgttt ttaaagctcg ttctgcctga cgggtgagat gtaagttttt cttttcgttt
     1741 ccggcttcag catttggatt tggcggactc agtatttcaa ctttctttcg caaataatct
     1801 aaatcaatat ctaaattatt taaaattgta attgcttttc cgttgccgtc tctcaaaatg
     1861 cctaacatta aatgctcagt tccaataaag tcatgaccta atcgtaaagc ttcttcttta
     1921 ctgtaagtaa tgacatcttt gactcttgga gaaaaattat cgtccattgc gtatatttta
     1981 ataggtaaat ttagtaaata tgattggcaa atgcaaaaat cattccgatg cctatctaat
     2041 gtcagctaat tgacaaaaaa aagttaataa taaaagttaa agtagcgtat aattattaac
     2101 taaaaaacac cttcattttg ttaataaatc attgaaatta ctttactgat ttgctttaaa
     2161 aaagcatgga aattcgtata ttagcacgtt ttgaaataac tataatttta atatataaat
     2221 acttatgtct gaaggagaaa agttaattcc tattaacatc gaagacgaaa tgaaatcagc
     2281 ttacatcgat tattcgatgt cggttattgt gtcacgtgct ttgccggatg ttagagatgg
     2341 tttgaaacca gtacaccgta gagttcttta cggaatgtat gatttaggag ttttttcgaa
     2401 tagagctcac aaaaaatccg ctagaatagt tggagaagtt ttaggtaaat atcacccaca
     2461 cggcgataca tcggtttatg atgctatggt tcgtatggct caggaatgga gtttacgtta
     2521 tttattagtt gatggtcaag gtaattttgg ttcagtagat ggtgacagcc cggcagcaat
     2581 gcgttatact gaagccagga tgcaaaaaat ttcggaagaa attatggctg atatcgaaaa
     2641 agaaactgtt gattttcaat taaactttga cgatacgtta tatgaaccta aagttatgcc
     2701 gacaaaagtt ccaaatcttt tgataaatgg agcttccggt attgccgtag gtatggcaac
     2761 caatatgcct cctcataatt taaccgaggt tgtcaatgga acgttagctt atattgacaa
     2821 tactgaaatt gagattgatg aattaatgga ctacatcaaa gctccggatt ttccaacagg
     2881 cgggattatc tatggctatg atggtgttcg tgaggcattc aaaacaggtc gtggacgtat
     2941 tgttatgcgt gctaaaacag gttttgaaga aagtaatggt agagaagcta tcatcgtaac
     3001 tgaaattcca tatcaggtca ataaggcgga tatgattaaa aaaacagccg atttagttaa
     3061 cgataagaaa attgaaggta tttcaaacat tcgagatgaa tcggatagaa atggtatgcg
     3121 aatcgtttat gaattaaaac gagatgcagt tccaaatgtc gttttgaata cactatataa
     3181 atatacacaa ttacaatctt cgttcagtgt aaacaatatt gcattggtaa acggtcgtcc
     3241 tcaaatgttg aatttgaaag aattgattca ctattttgtg gaacaccgtc atgatgttgt
     3301 agttcgaaga gctaagttcg atttgcgtaa agcagaagaa agagctcata tacttgaagg
     3361 tttaattatt gcatctgata atatagatga agtaattgct ttgattcgta gttcaaaaga
     3421 cggagacgaa gccagaacaa agttaattga gcgtttttca ctttcagaaa ttcaagcacg
     3481 agcaattgtt gaaatgcgtt tgcgtcaact taccggtctt gagcaagata aattgcgtgc
     3541 cgagtacgaa gatatcatga aattgattac tcacttaaga gaattattag cgaataaaga
     3601 cttgagaatg caagttatca aagatgagtt agttgaaatt cgtgataaat atggtgacga
     3661 aagacgttcg gttattgaat attcaggtgg agatgttagt attgaagatt taatagccga
     3721 cgaaaatgta gtaattacaa tttctcatgc cggttatatt aaacgtacaa acttatctga
     3781 atataaaaca caaaatagag gaggagttgg tcaaaaaagt gc
//
LOCUS       DAOTCW010000191         3778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_19313_length_3778_cov_49.840451, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000191 DAOTCW010000000
VERSION     DAOTCW010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19313_length_3778_cov_49.840451"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1856)
                     /locus_tag="P5335_11110"
     CDS             complement(<1..1856)
                     /locus_tag="P5335_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRZ75473.1"
                     /translation="MDRFSFLNAAHTQFFADLYEQYTVNPDAVEPSWRAFFQGFDFGM
                     ESSSEEQAINQLADFSSGNQDYSLVSEKFQKEFKVLKLIDGYRTRGHLFTKTNPVRER
                     RTYAPTLALENFELSASDLDTVFDAAKVLGHQPSTLRDIVKHLENVYCQSIGIEYMYI
                     RKPEVIEWIQKKLNINDNLPNFSADEKKHILHKLNEAVSFENFLHTKYVGQKRFSLEG
                     GESIIPALDALIEAAADKGVEKFVMGMAHRGRLNILANIFRKETQDIFSEFDGKDYDK
                     EYFDGDVKYHLGLTADRKTKSGKNININLAPNPSHLETVGAVIEGITRAKQDKYFPND
                     FSKVLPIAVHGDAAVAGQGIVYEIVQMAQLDGYKTGGTIHIVINNQVGFTTNYLDARS
                     SVYCTDVAKVTLSPVLHVNADDAEAVVHAMLFALDFRMTFGRDVFIDLLGYRKYGHNE
                     GDEPRFTQPVLYKIIARHKNPRDIYAEKLIIDGIVNQAYITKIENEYKANLDQNLEAS
                     RKKELTVITPFMQNEWEGFIQVSDDEMLKKVNTTFDKKKLDAIAETISTLPSDKKFIS
                     KISKIVTDRKTMYDNDAIDWGTAETLAYGTLLTEGYDVRISGQDVERGTFSHR"
     gene            1976..2503
                     /locus_tag="P5335_11115"
     CDS             1976..2503
                     /locus_tag="P5335_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75474.1"
                     /translation="MRKLIFFKRVSVFLFLFLGLNVSFAQDNPAPTSDFWKKVRFGGA
                     IGMNFGSNYTEVTLAPSAIYDFNRYFSLGVGLRGSYAQEKNYYESWIYGGSLIALSNP
                     IDFLQLSVELEQLRYNVTNEYPITYEDDFWNTGLYLGAGYFSGNVTVGIRYNVLYNSD
                     EIYAEPWLPFVRVYF"
     gene            complement(2487..2840)
                     /locus_tag="P5335_11120"
     CDS             complement(2487..2840)
                     /locus_tag="P5335_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75475.1"
                     /translation="MNYLAPTSTDHLLTEAEKENLYLKLIEQLNKDFNFANEPIDVPL
                     STSPNELKIQLHEKVYRLIQYKFAEYLNLLYIIDVSETEVKKLDGSDIAELAEQVAFL
                     ILKREWQKVWFRNKL"
     gene            complement(2941..3636)
                     /locus_tag="P5335_11125"
     CDS             complement(2941..3636)
                     /locus_tag="P5335_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75476.1"
                     /translation="MKTVNFVLVLLLFFGMQNVIAQADVKENVAMIKKNLADSKEKIK
                     KYEWIETTTVFYKGEQKSVIQKQCYYAVDGKLTKVETGGSTPAEKKRGIKGKIAENKK
                     EEMSDYMKKAIEKIHTYLPPAPEKIQKIYNDGKTTIQIVEPNTKFKLSFPDYNESSDM
                     LSVTVNKPNQKIMSLEVSTSVDDPKEKVVFNVNYSDLPDGTQYASKTILDAQAKDVKI
                     IIENSGFKNASGK"
BASE COUNT         1120 a          663 c          595 g         1400 t
ORIGIN      
        1 cgatgtgaaa aagttcctct ttctacatcc tgaccggaaa tacgaacatc atatccttcg
       61 gttaataatg tcccgtaggc caatgtttca gccgtacccc aatcaatcgc atcgttgtca
      121 tacatcgttt ttctatcggt aacaatctta ctgattttac taataaattt tttatccgat
      181 ggtaacgtag aaattgtttc tgcaatggcg tctaatttct ttttgtcaaa agtagtatta
      241 acttttttca acatttcatc atcgctcact tgtataaatc cttcccattc attttgcata
      301 aacggtgtga taacggtcaa ttctttttta cgtgaagctt ctaaattttg atctaagttt
      361 gctttgtatt cgttttctat ttttgtaata taggcttggt ttacaattcc gtcgataatt
      421 aatttctcag cataaatatc tcttggattt ttgtgacgag caatgatttt gtataaaacc
      481 ggttgcgtaa agcgaggttc atcaccttca ttgtgcccat attttctgta tcctagcaaa
      541 tcaataaata catctcttcc gaaagtcatt cggaaatcta aagcaaaaag catagcatga
      601 acaactgctt ctgcatcatc tgcattgaca tgaagaacag gcgacaaagt aactttggca
      661 acatcggtac aatataccga agaacgagca tctaaatagt ttgtagtaaa accaacttga
      721 ttatttatta cgatatgaat tgttccacct gttttatacc catccaactg agccatttga
      781 actatttcat aaacaattcc ttgaccggca actgcggcat caccatgcac ggcaatgggc
      841 aatacttttg agaaatcatt cggaaaatat ttatcttgtt tagctctggt aattccttca
      901 ataacagctc ctaccgtttc taaatgggaa ggatttggtg ctaaattaat attgatgttt
      961 tttccggatt ttgtttttct gtcagctgtt aatcccaaat ggtattttac atcgccatca
     1021 aaatattctt tgtcataatc tttaccatca aattctgaaa aaatatcttg cgtttctttt
     1081 ctgaaaatat tggctaaaat gtttaaacgg ccacgatgag ccattcccat cacaaatttt
     1141 tcaacccctt tatcagcagc ggcttcaatc aaagcatcta aagcagggat aattgactct
     1201 cctccttcta atgaaaatct tttttgtccc acatatttgg tgtgcaaaaa gttttcaaag
     1261 gaaacagctt cgtttagttt atgcagtata tgttttttct catccgcaga aaaatttggt
     1321 aaattatcat tgatatttaa tttcttttga atccattcaa taacttccgg tttacggatg
     1381 tacatgtatt caataccaat tgattgacaa tatacatttt caagatgctt cacaatatct
     1441 ctcaaggtag acggctgatg acctaacact ttagcggcat caaaaactgt atctaaatca
     1501 gaagcagaaa gctcaaaatt ctccaaagct aaagtcggag cataggttct tctttcccgt
     1561 accggatttg ttttagtaaa taaatgtcct cgggttctat agccatcaat taattttaga
     1621 actttaaatt ctttttgaaa cttttcagat accaaactat aatcttgatt tccggatgaa
     1681 aaatccgcta attgattaat tgcttgttct tcactggaag attccattcc gaaatcgaac
     1741 ccctgaaaaa aggctcgcca acttggttca acagcatccg gattaacggt atattgttcg
     1801 tataaatcgg caaagaattg agtgtgtgct gcgtttaaaa aggaaaacct atccataatt
     1861 agtagtaata aaccttattt gttaaaaata gttttgcaaa agtacaatat ttgcaataat
     1921 cttttattta tttttacata cttttatgac tgtaatttgc taaaaaggta taggtatgag
     1981 aaaattaata ttttttaaaa gagtaagtgt ttttttattt ctttttttag gattgaatgt
     2041 ttcattcgct caagataatc ctgctccaac cagtgatttt tggaaaaaag ttcgttttgg
     2101 tggagctatt ggaatgaatt tcgggagtaa ttatacagaa gtcactttag ccccgagtgc
     2161 catctatgat tttaaccgtt attttagttt aggtgttggc ttgcgaggca gctatgctca
     2221 agaaaaaaat tactatgaat cttggattta tggtggttca ctcattgctc tatccaatcc
     2281 aattgatttt ttacaacttt ctgttgaatt agaacaattg cggtacaatg tgacaaatga
     2341 atatcctatt acttatgaag atgacttctg gaataccggg ttgtatttag gtgccggtta
     2401 tttttcagga aatgtaacgg tgggtattcg atataatgtt ctttacaaca gtgatgaaat
     2461 ctacgcggaa ccttggttac cgtttgttag agtttatttc taaaccatac tttttgccat
     2521 tctcttttca aaatcaaaaa agcgacttgt tctgccaatt cggcgatgtc tgaaccatcg
     2581 agttttttta cttcagtttc ggacacatca atgatataca gcaagtttaa atattcagca
     2641 aatttgtatt gaatgagtcg gtacactttt tcgtgcaatt gaattttcaa ttcattgggt
     2701 gaagtactta aaggaacatc tatcggctca ttggcaaaat taaaatcctt gttgagttgc
     2761 tcaatcaatt tgaggtagag gttttctttt tcagcttcgg ttaagaggtg atctgttgat
     2821 gttggagcta gataattcat aaaaaagtta gtcttaaact agcttgtaaa gatacaaaat
     2881 agaataagac taaccgtttt caattgaatt tcaaaaaaca cttattaatg ctgtgaactt
     2941 ttattttccg gaagcatttt taaatcctga attttcgata attattttta catctttggc
     3001 ttgtgcatca agaatggttt tgctggcata ttgagttcca tccggtaaat cactataatt
     3061 aacattaaaa actacttttt ctttcgggtc atcaacagag gtgctcactt ctaaagacat
     3121 gattttttga tttggcttat tgacagtgac cgaaagcata tcactagact cgttataatc
     3181 aggaaaactg agcttaaact tagtgtttgg ttcaactatc tgaattgtgg tttttccgtc
     3241 attatatatt ttttgaattt tttccggagc cggcggtaaa taggtatgga ttttttcgat
     3301 tgcttttttc atataatcgg acatttcctc ttttttgttt tcagcaattt ttccttttat
     3361 tcctctcttt ttttctgcag gagttgagcc gcctgtctcc acttttgtta actttccatc
     3421 cacagcataa taacattgct tttgaataac agatttttgt tcccctttat aaaaaaccgt
     3481 ggtggtttca atccattcat actttttaat tttctcctta ctgtcagcta gatttttctt
     3541 aatcattgct acattttctt tgacatccgc ttgggctatc acattttgca tcccaaaaaa
     3601 gagaagcaga actaatacaa agtttactgt tttcattttt ttatttttta tattgaaata
     3661 ataatcctaa acaaatagtt aattttaatg aagattagaa taaatttatt ttttgacttt
     3721 tatattattt ttcataatca cctatgattt atttaatttc caccaccaat ttaggtat
//
LOCUS       DAOTCW010000192         3680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_20032_length_3680_cov_39.192552, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000192 DAOTCW010000000
VERSION     DAOTCW010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20032_length_3680_cov_39.192552"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="P5335_11130"
     CDS             complement(<1..841)
                     /locus_tag="P5335_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRZ75477.1"
                     /translation="MNEILQYFLLLPLFGFVVSLLLPESKEKLISSIAFGTVFLQLVG
                     IVVFIIVWIFQGADDLNFKELTLIKTSNYEFFIDFYFDKIAAVYLFVGAVLTSMITTY
                     SRYYLHREKGYKRFFNTVLFFFFGYNLAILSGNFETLFIGWEIIGISSFLLIAFYRER
                     YLPVKNAFKVFSIYRIGDVGFILAMWASHHLFHENITFMKLNNFDLVSEHLQNHSSIG
                     FFIALCLACAAAAKSAQIPFSSWLPRAMEGPTPSSAIFYGSLSVHLGVFLMLRTYPFW
                     EHQT"
     gene            complement(843..3341)
                     /locus_tag="P5335_11135"
     CDS             complement(843..3341)
                     /locus_tag="P5335_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HRZ75478.1"
                     /translation="MKSTKHGFDEHKVLHHLTHYLPAQNPLKDFVHHNTLHAFQHKNF
                     HEALEEATAIFGYKTYLSLTDFRKRFANLEIKETVLDSILIQLKGEEHWQLWKNKLLQ
                     NYYDESVNPRIGSLRANWKKEYAINLEKSTHSLLFRVLCSYLDQGIAMWNFPVHENGF
                     LASIRTLENNSFKGIFSSKRAIELLQKDASITQLLEIVVGKEVLFEQYLFDQQFSHPG
                     WSGMIATIESNPSSLLDAKAITFEELIKFELLLEIDTLDRKFNGNWSPLGLKVRENEY
                     NLFENVKHNELFEVLSLWQQAFEWSFYDEVLINLKHTHETISTETPSFQGLFCMDDRE
                     CSFRRHIEHLDTKAETFGTAAFFNFEFYFQPVNGKFYTKLCPAPVTPKFLIKEVQRKK
                     KQAKDLLYHKQTHSLLFGWIISQTLGFSSAFKLFLNIFKPSMSPATTASFKHLHKKAK
                     LIVENTNVNQREDNLQIGFTVEEMTDRLEGLLKSIGLVKNFSPIVYIVGHGATSVNNT
                     HYAGYDCGACCGRPSSVNAKVAKMAANHTKVRELLKLRGIIIPETTQFIPALHDTTRD
                     EIVFYDQDILTPENKKLHLQNEIVFAKALVNNAKERSRRFDTIDSKLSLSKVHEKVKR
                     RSLSLFEPRPELNHATNAMCIVGRRSLSKQLFLDRRSFLNSYDYEVDPSGDYLSIILG
                     AVAPVAGGINLEYYFSRVDNQKLGAGSKLPHNVMGLIGVANGIDGDLRPGLPSQMIEV
                     HDPLRILVIAEHFPEKVNYAITKNPSTYEWFKNDWVKLIAIHPQTKEVFEFKNEAFVS
                     YKTVQEHVNLIDNELDLALTTKENLPIGILTKAY"
     gene            complement(3447..>3680)
                     /locus_tag="P5335_11140"
     CDS             complement(3447..>3680)
                     /locus_tag="P5335_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75479.1"
                     /translation="STQQNHFNGFSITNQYKDKGESEIDEAHVDFEVTFTSHSPFTFL
                     PYQKFISSNELNRFDFLKKPLYDLFCNWKFHLI"
BASE COUNT         1232 a          671 c          595 g         1182 t
ORIGIN      
        1 ttgtttgatg ttcccaaaaa ggataggtac gcaacatgag aaaaactccc aaatgaaccg
       61 aaagtgagcc ataaaaaatg gcactcgaag gcgtaggtcc ctccatcgct cttggcaacc
      121 aggaagaaaa cggaatttgt gccgattttg ctgccgcagc acaagccaaa cacaaggcta
      181 taaaaaagcc aattgaagaa tgattttgta aatgttcact caccaaatca aaattattta
      241 gcttcataaa agtaatattt tcatgaaata aatggtggct tgcccacatg gccagtatga
      301 aaccgacatc gccaattctg taaatagaaa atactttaaa ggcatttttt acaggcaaat
      361 agcgttcccg ataaaaagct atcagaagga atgaagaaat tccaatgatt tcccacccaa
      421 tgaaaagggt ttcaaaattt ccggaaagta tagccaaatt ataaccgaaa aagaaaaaaa
      481 gtacggtatt aaaaaatcgt ttataccctt tttctcgatg caaataatac cggctataag
      541 tggtaatcat agaagttaac actgcaccga caaacaaata aactgccgct attttatcaa
      601 aataaaaatc aatgaagaat tcgtaatttg atgtttttat aagtgtcagt tctttaaagt
      661 tcaaatcatc ggcaccttga aaaatccaaa caatgataaa aaccactatt cctacaagtt
      721 gtaaaaaaac ggtgccaaat gctattgaag aaattaattt ttctttagac tcaggcaata
      781 gcaagcttac aacaaaacca aaaagcggta gtaataaaaa atattgaagt atctcgttca
      841 tattagtaag ctttagttaa aattccaata ggaaggtttt ctttcgttgt taacgccaaa
      901 tccagttcgt tatcaattaa attcacatgc tcttgaactg ttttgtagct aacgaacgct
      961 tcgtttttaa attcaaaaac ttcctttgtt tgcggatgaa tggctatcag ttttacccaa
     1021 tcatttttga accactcata ggttgaaggg ttttttgtaa ttgcatagtt tactttttcc
     1081 ggaaaatgct cagcaatgac caaaattcgc aacggatcat gcacttcaat catttgactg
     1141 ggcaagccgg gacgcaaatc gccatcgata ccgttagcaa ctccaattaa tcccattacg
     1201 ttgtgaggca attttgaacc tgctcctaat ttttggttat ccactcttga aaaataatat
     1261 tccaagttga ttcctccggc caccggagca actgcaccaa gtataattga caaataatca
     1321 ccggatgggt cgacctcata atcataggaa ttcaaaaaag agcgacgatc taaaaaaagt
     1381 tgtttcgaca agcttctcct tcctacgata cacatcgcgt tggttgcatg atttagttcc
     1441 ggacgaggct caaacaatga caacgaacgt cttttaactt tctcatgaac tttactcaag
     1501 gatagctttg aatcaatcgt gtcaaaacgt ctcgaacgtt ctttggcatt gttgactaaa
     1561 gcttttgcaa aaacaatttc attttgaaga tgtaattttt tattttcagg tgtgagtata
     1621 tcttgatcat aaaacacaat ctcatctcgt gtggtatcat gcaaagccgg aataaactgt
     1681 gtcgtttctg gaattattat acctctcaac tttaatagct ctcgaacttt tgtatgattt
     1741 gctgccattt tagcaacttt agcatttacc gaactaggtc tgccacaaca agccccacaa
     1801 tcataacccg catagtgtgt attgttaaca cttgttgctc cgtgaccgac gatatataca
     1861 attggagaaa agtttttgac taaacctata cttttaagta atccttccaa tctatcggtc
     1921 atttcctcaa ctgtaaaacc aatttgaaga ttatcttcac gctgatttac attcgtattc
     1981 tctacaatca acttcgcttt cttatgtaaa tgcttaaatg aagctgttgt agccggtgac
     2041 attgatggtt taaagatgtt taagaacaac ttaaaggcag atgagaatcc taacgtttga
     2101 ctaataatcc aaccaaacaa taacgaatgt gtttgtttgt gatagagtaa gtcttttgct
     2161 tgctttttct ttcgttgaac ttctttgatt aggaattttg gagtaaccgg agccggacat
     2221 agttttgtat aaaactttcc gtttaccggt tgaaaataga attcaaagtt gaaaaatgcc
     2281 gctgttccaa atgtttccgc ttttgtatct aaatgttcaa tgtgtcttcg aaacgagcac
     2341 tctcgatcat ccatacaaaa taatccttga aaacttggtg tttcagttga gatggtttcg
     2401 tgcgtatgtt ttaagtttat taaaacctca tcataaaaac tccattcaaa agcctgttgc
     2461 caaagagaca atacttcaaa taattcgttg tgttttacat tttcaaacag attgtattca
     2521 ttttctctta cttttaaacc taatggtgac caattaccat tgaattttct atccaaagtg
     2581 tcaatttcta aaagcaactc aaattttatg agttcctcga aagtaattgc ctttgcatct
     2641 aacaacgaac tcggattgga ttcaattgtt gcaatcatgc cgctccaacc cggatgtgaa
     2701 aattgttgat caaacagata ctgttcaaac aagacttctt ttccaacaac aatttctaat
     2761 aattgtgtga ttgaagcatc tttttgaagt aattcaatag ctctttttga tgagaaaata
     2821 cctttaaagg aattattttc aagtgttcgt atggaagcta aaaagccatt ttcatgaacc
     2881 ggaaaattcc acatcgcaat tccttgatct aaatagctac acaaaacccg aaacaacaaa
     2941 ctgtgtgttg atttttcgag atttatggca tattcttttt tccaattggc ccgtaaactt
     3001 cctattcttg ggtttacaga ctcgtcataa taattttgaa gtaatttatt cttccaaagt
     3061 tgccaatgtt cctctccctt taattgaatc agtatgctat ctaaaactgt ttctttaatt
     3121 tctaaattag caaatcgctt tctaaaatca gttaatgaaa gataggtctt gtaaccaaaa
     3181 atagcggttg cttcctctaa agcctcgtgg aaatttttat gctgaaaggc atgaagtgta
     3241 ttgtgatgca caaaatcttt caacggattt tgggcaggca aataatgcgt taagtgatga
     3301 agaactttat gttcatcaaa accatgtttt gttgatttca taatgaaaat taacagttga
     3361 aaattcagtt gtagtatttt taaagcaatc ctaaattctc ttagaaagta aagaaataga
     3421 ctctaaaaaa taaaaatgac taactgttaa atcaaatgaa acttccaatt acaaaataaa
     3481 tcgtaaagtg gttttttgag aaaatcgaac ctatttaatt cattagaact aataaatttt
     3541 tgatagggta aaaatgtaaa tggagaatga gatgtaaagg taacttcaaa atctacatga
     3601 gcttcatcaa tttcagactc gcctttgtcc ttgtattggt tagtaatact aaatccgtta
     3661 aaatgatttt gctgagttga
//
LOCUS       DAOTCW010000193         3660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_20196_length_3660_cov_46.819695, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000193 DAOTCW010000000
VERSION     DAOTCW010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20196_length_3660_cov_46.819695"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="P5335_11145"
     CDS             <1..123
                     /locus_tag="P5335_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRZ75480.1"
                     /translation="GGSAMDAIARQPEASGKIQTAMLIAAALIEGIGFAALFAS"
     gene            208..708
                     /locus_tag="P5335_11150"
     CDS             208..708
                     /locus_tag="P5335_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRZ75481.1"
                     /translation="MDKLIEQFSLGLFVWMLVIFIGLIFLMRKFAWKPILDAVNEREE
                     GIRNALLSAENAKKEMLNLQSSNEKLVAEARAERDAMMKEAREIKEKMINDAKSEAQT
                     QGEKMIAQAKAAIESEKNAAMAELKNQVSTLSLEIAEKVLRDELSNKESQTKLVEKML
                     GDAKLN"
     gene            708..1241
                     /gene="atpH"
                     /locus_tag="P5335_11155"
     CDS             708..1241
                     /gene="atpH"
                     /locus_tag="P5335_11155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRZ75482.1"
                     /translation="MRMSRAAIRYAKAIIENAVSNGRADAVNQDMNLILSTVSSNEEL
                     QHFLISPVVKPQEKYTAIVEIFAAVQPDTKGLFLLLKENKRFEILAEIAQQYNAQHDV
                     LNGIEVAKVTTAFPITPDLEAKVLAKIKEFSMSKITIENIVDPAIIGGFILRIGDQQY
                     NASVSNRLQELKREFSN"
     gene            1265..2842
                     /gene="atpA"
                     /locus_tag="P5335_11160"
     CDS             1265..2842
                     /gene="atpA"
                     /locus_tag="P5335_11160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRZ75483.1"
                     /translation="MAEIKPAEISAILKKQLSGFESGASLEEVGTVLQVGDGIARVYG
                     LSNAQYGELVQFDNGLEAIVLNLEEDNVGVVLLGPSTGIKEGSTVKRTQRIASLKVGE
                     QMVGRVVDTLGNPIDGKGPIGGDLYEMPLERKAPGVIFRQPVTEPLQTGIKSIDAMIP
                     VGRGQRELVIGDRQTGKTTVCIDTILNQKEFYDAGQPVFCIYVAIGQKASTVAGIAKT
                     LEDKGAMAYTIIVAANASDPAPMQVYAPFAGAAIGEYFRDSGRPALIIYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKVIADDDIAKNMNDLPESLRPIVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLDGDLFNSGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQFRELEAFAKFGSDLDAVTLNVIEKGKRNVEILKQAVN
                     DPYKVEDQVAIIYAGSKNLLRNVPVIKVKEFERDFLEYLNNKHKDTLNALKAGKFTDE
                     ITDVLDNAAKEVSAKYN"
     gene            2915..>3660
                     /gene="atpG"
                     /locus_tag="P5335_11165"
     CDS             2915..>3660
                     /gene="atpG"
                     /locus_tag="P5335_11165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRZ75484.1"
                     /translation="MANLKEIRNRITSVSSTMQITSAMKMVSAAKLKKAQDAITAMRP
                     YAEKLTEILQSLSASMDGEVGGDYTSQREVNKVLVVAITSNRGLCGAFNTNVIKQVKV
                     VADSYVGKQVDIFAIGKKGNDVLRKTHNVVENKSDVFDHLTFDNVAAISDLLSEKFIS
                     GAYDKIEVVYNQFKNAATQIVQTEQFLPLAPVVGAATASSDYIFEPSKEEIVLTLIPK
                     SLKTQLYKSIRDSFASEHGARMTAMHKATD"
BASE COUNT         1271 a          585 c          752 g         1052 t
ORIGIN      
        1 ggtggttcag caatggacgc tatcgctcgt caaccagaag cttctggaaa aattcaaaca
       61 gctatgctta ttgcagcagc tcttattgaa ggtattggtt tcgctgcgtt attcgcatct
      121 taatctaaaa aaattaacaa cagttatgac ggttggttat aactgttgtt tttaaaaaaa
      181 ttaaaatttt ctaaattatt tatataaatg gataagttaa tagaacagtt ttcacttggt
      241 ttgtttgttt ggatgcttgt catctttatt gggttgattt tcttaatgag aaaattcgct
      301 tggaaaccaa ttttagatgc ggttaacgaa agagaagaag gaattagaaa tgcattatta
      361 tctgctgaaa atgcaaagaa agaaatgcta aatcttcaat cttctaatga aaagttagta
      421 gcagaagcaa gagcagaacg tgatgccatg atgaaagaag ctcgtgaaat caaagaaaaa
      481 atgattaacg atgcaaaatc agaagctcaa acgcaaggtg agaaaatgat tgctcaggca
      541 aaagccgcta ttgaaagcga aaaaaatgca gcaatggctg agttgaaaaa tcaagtttca
      601 accttgtcat tagaaattgc agaaaaagta ttaagagacg aattgtctaa caaagaatct
      661 caaaccaaat tggttgagaa aatgttaggc gatgctaaat taaattaatc cgtatgtcaa
      721 gagctgcgat tagatatgcg aaagcaatta tagaaaatgc tgtttccaac ggaagagccg
      781 acgcggtgaa tcaagacatg aacttgattt taagtaccgt aagttcgaac gaagagttac
      841 aacatttttt aatcagtcca gtggtaaaac cacaagaaaa atatacagcg attgttgaaa
      901 tttttgctgc ggttcaaccg gatactaaag gattgttttt attgcttaag gaaaacaaac
      961 gttttgaaat tttagcggaa attgcccaac aatacaacgc tcaacacgat gtattaaacg
     1021 gaattgaagt agctaaagtc actaccgctt ttcctattac tcctgattta gaagcgaaag
     1081 ttttagcgaa aatcaaagag ttttcaatga gtaaaatcac catcgaaaac attgtagatc
     1141 ctgctattat cggaggattt attttaagaa tcggtgacca acaatacaat gcgtctgttt
     1201 caaacagatt gcaagaatta aagagagagt ttagtaatta atattccaaa aaagatagag
     1261 cacaatggcg gaaattaaac ctgctgaaat ttcagcaata ttaaaaaaac aattatctgg
     1321 ttttgaatca ggtgcatcgt tagaagaagt aggaacagta cttcaagtag gtgatggtat
     1381 tgctcgtgtt tacggcttat caaatgctca atacggagag ttagtgcaat tcgacaacgg
     1441 attggaagca atcgtattga acttggagga agataatgtt ggggtagtat tgttaggtcc
     1501 ttcaacaggt atcaaagaag gttctactgt aaagagaaca caacgtatcg cttcccttaa
     1561 agtgggtgag caaatggtag gtcgtgtagt agacacttta ggaaatccaa tcgatggtaa
     1621 aggaccaatc ggtggagatc tttatgaaat gccattggaa agaaaagctc ccggagtaat
     1681 tttccgtcaa ccggtaactg aaccgttgca aacaggtatc aaatcaattg atgcaatgat
     1741 tccggtagga agaggtcaac gtgagttggt aattggtgac cgtcaaacag gtaaaacaac
     1801 tgtttgtatc gataccattt taaatcaaaa agaattttat gatgcaggtc aacctgtgtt
     1861 ttgtatctac gtagcaattg gacaaaaagc gtcaactgtt gccggaattg caaaaacatt
     1921 agaagacaaa ggagcaatgg cttatacgat tatcgtagct gccaatgctt ccgatcctgc
     1981 accaatgcaa gtatatgctc catttgccgg agcagctatc ggtgaatatt tccgcgattc
     2041 aggtcgtccg gctttaatta tctatgacga tttgtctaaa caagctgttg cttaccgtga
     2101 ggtttctctt ttgttaagaa gaccaccggg acgtgaggct tatcctggag acgttttcta
     2161 ccttcactca agattgttag aaagagctgc aaaagtgatt gcagatgatg atatagctaa
     2221 aaacatgaac gatttaccgg aatctttgcg tccaatcgta aaaggtggtg gttcattaac
     2281 cgctttacca attatcgaaa cacaagcagg tgacgtttct gcgtatatcc caaccaacgt
     2341 aatttcgatt acagatggtc agattttctt ggatggtgat ttgtttaact caggggttcg
     2401 tccggctatt aacgtaggta tttcggtatc tcgtgttgga ggtaacgctc agattaaatc
     2461 aatgaaaaaa gtagcaggta ctttgaaatt agaccaagct caattccgtg aattggaagc
     2521 gtttgctaaa tttggttcag atttggatgc cgttacttta aacgtaattg aaaaaggaaa
     2581 aagaaacgtt gaaatcttaa aacaggcggt aaacgaccct tataaagtgg aagatcaagt
     2641 agcgattatc tatgcaggat ctaaaaactt attgagaaac gttccggtta ttaaagtaaa
     2701 agaattcgaa agagatttct tggaatactt gaacaacaag cataaagaca ctttgaatgc
     2761 attaaaagca ggtaaattca cagacgaaat taccgatgtt ttagacaatg cagcaaaaga
     2821 agtttcagcg aaatataact aaaaagtatt aagtagtaag tatcaagaat taagatgtct
     2881 tgatacttac tgcttaaatc ttaatacatt taaaatggca aacttaaagg aaatacgtaa
     2941 tagaattact tccgtttcat cgacgatgca aatcacatcg gctatgaaaa tggtttctgc
     3001 agctaagcta aaaaaggctc aagatgccat tacggcaatg cgaccttatg ccgaaaaatt
     3061 aacggagatt cttcaaagct taagtgcttc gatggatggc gaagttggag gagattatac
     3121 ttcacaacgt gaggtcaaca aagtattagt cgttgctatc acttcaaaca gaggtttatg
     3181 tggtgctttt aataccaacg ttatcaaaca agtcaaagtg gtagccgatt cttacgtagg
     3241 aaaacaagtg gatatttttg ccattggtaa aaaaggaaat gatgttttac gcaaaacaca
     3301 taatgtagtt gaaaacaaaa gtgatgtttt tgatcattta acttttgaca atgttgccgc
     3361 tatttctgat ctattatctg aaaaatttat ttcaggtgct tacgataaaa ttgaagtagt
     3421 atataatcaa ttcaaaaatg cagctacgca aatcgttcaa actgaacagt ttttgccttt
     3481 agctcctgtt gttggtgctg caaccgcaag ttcagactat atttttgaac catcaaaaga
     3541 agaaatagtg ttgactttga ttccgaaatc attaaaaaca caattgtaca aatctattcg
     3601 cgattctttt gcttctgaac atggtgctcg tatgaccgcg atgcacaaag caaccgataa
//
LOCUS       DAOTCW010000194         3604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_20670_length_3604_cov_42.837983, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000194 DAOTCW010000000
VERSION     DAOTCW010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20670_length_3604_cov_42.837983"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="P5335_11170"
     CDS             complement(<1..1487)
                     /locus_tag="P5335_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRZ75485.1"
                     /translation="MKIFDAKNIKLIDKLTINKQQINSLDLMERAALQAFLWIVNHFH
                     EKKTVFHIFCGVGNNGGDGLVIARMLKQNYFDVHVYIVPFSDKFSVDFDSNLERLKEC
                     NLTYDVINETAVFPDISENHIIIDAIFGIGLTHEMDLWIQKIIQKINYHQSFTISIDV
                     PSGLFLEKKTILAIHSDVVLTFQFPKLAFFLPDNEHFVSEIVILDIEWDYHVMDDIAT
                     HFFYSDLIEIKKRYNPIGKFSHKGTQGHALLIGGCFGKMGTMILASQAALRSGCGLAT
                     VLIPKCGYEILQTSSPEVMVITDTNYHKITSINFDFAPKAIGIGMGIGQDLDTQIAFH
                     SFLLSNNLPLVIDADALNILSDNLEWIDLVPKNSILTPHPKELERLIGSWQDDFEKIE
                     KTINFAQKYHLVILIKGAYTLIIDEKNVYINPTGNQALATGGSGDVLAGMITSLRAQG
                     YQSVDAALLGVYLHGKTADLAVKEMGYEGFIASDIIKYLGKAFLE"
     gene            1758..>3604
                     /gene="thrA"
                     /locus_tag="P5335_11175"
     CDS             1758..>3604
                     /gene="thrA"
                     /locus_tag="P5335_11175"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HRZ75486.1"
                     /translation="MKVLKFGGTSVANAQNIKLVLEIVEQKSMQDKLIVAVSAFSGVT
                     DLLLSAAQKASTKDDSYRDVVLQIEKKHLEAIKELVPANEQAALLHYCKKNISYLETL
                     LDGCYLLGELTLRTSDMIVSFGELLSSYIIAEALKQKVGNSNFKDSRELIKTNSNFGK
                     AEVNFDITNATIADYFKSNSSQVTVIPGFIASSSDGNTTTLGRGGSDYTAAIIAAALN
                     VKELEIWTDVNGMFTANPKLVKQAQPIEKITYEEAMELSHFGAKVLYPPTIQPVLSKN
                     IPIWIKNTFEPDAFGTYISSESNANGNPIKGISHIDKIALITLEGSGMVGVAGVSKRL
                     FEVLSNHSISIIFITQASSEHSICIGILNADADVAKTAIDSVFELEIAQHKINPCIVE
                     KDLCIVALVGEQMKNHQGISGKMFSTLGKNNVNIRAIAQGASERNISVVINENDVKKA
                     LNTLHERFFEDNTKQLNLFVMGVGNVGEKFIEQIHQQKKFLKENLKINLRVIALSNSR
                     KMVFDEEGISLKDWKTALDNGEKASKHDFIEKIKTLNLRNSIFVDITANEEVSKTYEH
                     YLRKNIAVVTCNKIACSSDYENYQNLKRLSRKYNAPFLFETNVGAGLPI"
BASE COUNT         1268 a          608 c          573 g         1155 t
ORIGIN      
        1 aactctaaaa aagcttttcc taaatattta ataatatccg aagcaataaa tccttcataa
       61 cccatctctt taacagctaa atcagcagtt ttcccatgaa gataaacgcc taataaagca
      121 gcatcaacag attgatatcc ttgagctcgt agacttgtaa tcattcccgc taatacatca
      181 ccactcccac cggttgcaag tgcttgattt cctgtcgggt ttatatatac attcttttca
      241 tcaataatta aggtataagc tcctttaatt aaaataacca aatgatattt ctgagcaaaa
      301 ttgatagttt tctcaatttt ttcaaaatca tcttgccaag aaccaattaa ccgttctaat
      361 tcttttggat gaggtgttaa aattgaattt ttaggcacta aatcaatcca ttctagatta
      421 tcagataaaa tatttaatgc atctgcatct attaccaaag gcaaattatt agaaagcaaa
      481 aaagaatgaa atgcaatctg agtatctaaa tcttgaccta tacccattcc gatacctata
      541 gctttaggtg caaaatcaaa atttattgaa gtgattttat gataatttgt atcggttatt
      601 accataactt caggtgagga ggtttgtaaa atttcataac cacatttagg aattaagaca
      661 gttgccaaac cacaaccgga tcgcaaagct gcttgtgaag ctaatatcat tgttcccatt
      721 tttccaaaac aaccgcctat taataaggca tgcccttgag ttcctttatg agaaaatttt
      781 ccaattggat tatatcgttt cttaatttct attaaatcag aataaaagaa atgtgtggca
      841 atatcatcca ttacatgata atcccattct atgtctagaa ttactatttc agaaacgaaa
      901 tgctcattat caggtaagaa aaaagcaagt tttggaaact gaaatgttaa aacaacatct
      961 gaatgtatgg ctaaaattgt cttcttttcc aaaaataatc cagaaggaac atcaattgaa
     1021 atggtaaaag attgatggta atttattttt tgaataattt tttgtatcca caaatccatt
     1081 tcatgtgtca atccaatacc aaaaatagca tcaataataa tatgattttc cgatatatca
     1141 ggaaaaacag cagtttcatt tattacatcg taagttaaat tacattcttt taatctctct
     1201 aaatttgaat caaaatcaac tgaaaacttg tcgctgaaag gaactatata aacgtgaaca
     1261 tcaaaataat tttgtttcaa cattcttgca ataacaagac catcaccacc attattccct
     1321 acgccacaaa aaatatgaaa cactgttttt ttttcatgaa aatgatttac aatccataaa
     1381 aaagcttgta aagcagcacg ttccatcaaa tctaacgagt ttatttgttg cttattaatt
     1441 gtcaatttat caatcagctt gatgtttttg gcgtcaaaaa ttttcatctg tcagtagaat
     1501 tagtgatttt ttgaggtatt cgtaaaagtt tgtgttttta tatttatgta tgtaaattta
     1561 ttacatttgt ttcagatgaa atcacaaaag aacataaatt atttattccc attattcaat
     1621 tcgttgaacg ggttttcttt tggtacatca actacgacta caaccccatt cggggtataa
     1681 ttttcacata tcatccgcag ctatgcttat tcgaaagaat aaaataaaat ctatgttttt
     1741 aagtaaaaaa taatcaaatg aaggtattaa aattcggcgg aacttcggtt gccaatgctc
     1801 aaaatataaa gttagttctc gaaattgttg aacaaaaatc aatgcaagat aaactaatcg
     1861 tcgctgtttc tgcctttagt ggtgtcaccg atttactttt atcagctgca caaaaagcat
     1921 ctaccaaaga tgattcttac cgcgatgtcg ttttgcaaat tgagaaaaag catctagaag
     1981 caattaaaga attggtaccc gcaaacgagc aagcagcttt attacattat tgtaaaaaga
     2041 acatttccta tttagaaact ttattggatg gttgttattt gttaggtgaa ttaacccttc
     2101 gaacttcaga tatgattgtc agttttggag aattattatc gtcttatatc atcgctgaag
     2161 ctttgaagca aaaagtaggc aacagtaatt ttaaagacag tagagaactc atcaaaacca
     2221 atagtaattt tgggaaagcg gaagtgaatt tcgatataac taacgcaacc attgccgatt
     2281 attttaaatc caattcgtca caagtaaccg taattccggg tttcatcgct tcgtcttccg
     2341 atggaaatac cactactttg ggtcgtggtg gttccgatta tacagcagca attatagcgg
     2401 cagcattgaa tgtaaaagaa ttggaaattt ggacggacgt caatggtatg tttactgcca
     2461 atccgaaact cgtgaaacaa gctcaaccta ttgaaaaaat tacgtatgaa gaagcaatgg
     2521 aattatcgca ttttggagca aaagtgcttt atccaccaac aattcagccg gttttgagta
     2581 aaaatattcc gatttggata aaaaatacgt ttgaaccgga tgcattcgga acctatattt
     2641 cctctgaatc taacgctaat ggaaatccaa ttaagggaat ttcacatatt gataaaattg
     2701 cgttgattac gttagaaggc tccggaatgg ttggcgttgc cggagtttcc aaacgtttgt
     2761 ttgaagtact ttctaatcat tcgataagca ttatttttat cacgcaagct tcgtcagaac
     2821 attccatttg tattggaatt ttaaatgcag atgctgatgt agcaaagact gcaattgatt
     2881 cagtttttga acttgaaatt gcacaacata aaatcaatcc atgcatcgtt gaaaaagact
     2941 tgtgcatcgt ggctttggtg ggtgaacaaa tgaaaaatca ccaaggaatc agcggaaaaa
     3001 tgtttagtac gttaggtaaa aacaacgtca acattcgtgc gattgctcaa ggtgcttccg
     3061 aacgaaatat ttcggtggtc atcaatgaaa atgatgtcaa aaaagcgttg aataccttac
     3121 acgaacgctt ttttgaagac aacaccaaac aattgaattt atttgtaatg ggcgttggaa
     3181 atgttggcga aaaattcatc gaacaaattc accaacaaaa aaaattctta aaagaaaatt
     3241 taaaaataaa tttacgtgtg attgctcttt ccaattccag aaaaatggtt tttgatgaag
     3301 aaggtatttc attaaaagat tggaaaacag cgttagataa tggtgaaaaa gcctcaaaac
     3361 atgattttat tgaaaaaata aaaacgctca atcttcgtaa cagtattttt gtggatatca
     3421 cggcaaatga agaggtctcc aaaacctacg aacattattt acgcaaaaat attgcggtag
     3481 ttacttgcaa caaaattgcc tgttcttctg actatgagaa ttatcaaaat ttaaaacgct
     3541 tatcgcgaaa atacaatgct ccctttttgt ttgaaaccaa tgtaggtgcc ggtttaccga
     3601 ttat
//
LOCUS       DAOTCW010000195         3580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_20864_length_3580_cov_40.853050, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000195 DAOTCW010000000
VERSION     DAOTCW010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20864_length_3580_cov_40.853050"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..509)
                     /gene="dtd"
                     /locus_tag="P5335_11180"
     CDS             complement(57..509)
                     /gene="dtd"
                     /locus_tag="P5335_11180"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRZ75487.1"
                     /translation="MKAVLQRVSKSSVTIDEKIVASIQKGIVILLGIEESDTNEDIEW
                     LSSKIVNLRIFDDTNGVMNLSVKEISGELIVVSQFTLHASTKKGNRPSYIKAAKPEIA
                     IPLYEKFIHQITLDLGKNVQTGQFGADMKVSILNDGPVTILIDTKNKE"
     gene            complement(531..1499)
                     /gene="rsgA"
                     /locus_tag="P5335_11185"
     CDS             complement(531..1499)
                     /gene="rsgA"
                     /locus_tag="P5335_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRZ75488.1"
                     /translation="MTGIVYKSTGSWYTVKADNNQIFDCRIKGKFRMKGIKSTNPIAV
                     GDVVDFEIDETSDAITGSIHHIDDRKNYIVRKSVNLSKQTHIIASNIDIVFLIVTINN
                     PITTTSFIDRFLVTAEAYHIKAVLLFNKIDTFDETMSDEQLFLQHVYTQIGYDCLRVS
                     AKNGKGLDQLKEMMQGKVCMFSGHSGVGKSTLVNALEPTLNLKTKEISDLHQQGQHTT
                     TFAEMFDLTFGSKIIDTPGIRGFGIVDMEKQEIKGYFPEFFKLEDQCKFNNCLHKEEP
                     NCAIKKALEEDKIAWSRYKSYLQILEGDEEQYRNDIYAEGRNLDLE"
     gene            complement(1526..2608)
                     /locus_tag="P5335_11190"
     CDS             complement(1526..2608)
                     /locus_tag="P5335_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HRZ75489.1"
                     /translation="MENKIELRTWLNDFELSHPLVIAGPCSAETEDQVLKIAHELKNS
                     DVSIFRAGIWKPRTRPGGFEGVGAIGLKWLQKAKAETGLLMAIEVANANHVQLALEHD
                     IDVLWIGARTTVNPFAVQEIADALKNTDKIVLIKNPVNPDLSLWIGGLERLYQANIQK
                     LGVIHRGFSTYEKTKYRNNPEWQIAIDLQNKFPDLPMICDPSHITGRRDMILEVSQQA
                     LDLNYDGLIIETHIDPDNAWSDAAQQVTPNVLKQIFKELKVRKTTDEADEFNQRMNQL
                     RTQIDDFDRKLLETLGSRMKIADKIGALKKEKNVAVLQNKRWQELLEKMILDGNQKGL
                     SEEFIIHLFKAIHQESISHQEKIINS"
     gene            complement(2663..3496)
                     /locus_tag="P5335_11195"
     CDS             complement(2663..3496)
                     /locus_tag="P5335_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRZ75490.1"
                     /translation="MDITIAIQGIKGSFHHQVAQEYFKKKGTILECNSFESVVKSMVE
                     SKANNGIMALENSIAGSIIPNYALIDRNNLHIIGEHYLDIQLDFMVLKGQTIDDIKEI
                     HSHPIALLQCGIFLSQYPTIKLVESSDTAAAAQNIKQNNLKGIAALASPVAAQLYELE
                     TLFSAIHTVKSNKTRFVVLKTKNKELPKEEINKASIKFELDDTPGSLATVLNVMNNCK
                     LNLTKIQSMPIIESPFQYSFFVDAVFEKYKHYEKAKKLLEIMTTHFKVLGEYKRGNQT
                     Q"
BASE COUNT         1069 a          651 c          542 g         1318 t
ORIGIN      
        1 tgcttttcgg aaattgtggt ttccaaattt aaaagaattc tgatataaaa aaaatattat
       61 tctttatttt ttgtatctat taaaattgtc acaggaccgt cgtttagtat tgacactttc
      121 atatcagccc caaattgacc ggtttgtaca tttttgccta aatcgagtgt tatttgatgt
      181 atgaattttt cataaagcgg aatggcaatt tcgggttttg ccgcttttat ataactgggt
      241 ctattccctt ttttggtaga agcatgaagt gtaaattgac taactacaat cagctctcca
      301 gaaatttctt taaccgaaag attcatcaca ccattagtat catcaaaaat tctgaggttt
      361 acaatttttg acgaaagcca ttcaatatct tcattcgtat cagattcttc tattcccaaa
      421 agaattacaa ttcctttttg aatggaagca acaatttttt catcaatagt tacagaagat
      481 tttgaaactc tttggaggac tgctttcatt ttttgggtga tgggaaaagg ttactctaaa
      541 tccagatttc gtccctcagc ataaatatcg ttgcggtatt gttcttcgtc gccttccaaa
      601 atttgaaggt aacttttata acgggaccaa gcaattttgt cttcttctaa tgcttttttg
      661 atggcacaat tcggttcctc tttatgcaag caattattga atttacattg gtcttccaac
      721 ttaaaaaact caggaaaata accctttatt tcctgttttt ccatgtctac aattccaaaa
      781 ccgcgaattc cgggggtgtc gattatttta gacccaaaag tcaaatcaaa catttcagca
      841 aaagttgttg tgtgttgtcc ttgctggtgt aagtcagata tttctttggt ctttaaattt
      901 aatgttggtt ccaaggcatt tactaaagtg gatttaccaa caccggaatg acctgaaaac
      961 atacaaactt tcccttgcat catttctttt aattggtcta atccttttcc gttttttgca
     1021 gaaacccgca aacaatcata tccaatttga gtataaacat gttgtaaaaa taattgttca
     1081 tccgacatgg tctcatcaaa agtgtcaatc ttattaaaaa gcaacactgc tttgatatga
     1141 taggcctcgg cagtaactaa aaatcggtca ataaaacttg ttgtggttat tgggttattg
     1201 atagtaacaa tcaaaaaaac aatatcaatg ttcgaagcaa tgatgtgtgt ttgtttagaa
     1261 agattgactg attttcgaac gatatagttt tttcggtcgt cgatatgatg gatcgaaccc
     1321 gtaatggcgt ctgaagtttc gtcaatttca aaatctacta catctcccac agcaattggg
     1381 ttggtacttt taattccttt catgcggaat ttacccttaa tccgacaatc aaagatttga
     1441 ttattatcgg ccttaacggt ataccaactt ccggtagatt tataaacgat tcctgtcatg
     1501 aagcaaatat acgagtttta aggaatcagg aattgataat cttttcttgg tgactgatac
     1561 tttcttggtg aattgctttg aataaatgaa taataaattc ttcgcttaac cctttttggt
     1621 tgccatctaa aatcattttt tctaataatt cctgccaccg tttgttttgt aaaacagcca
     1681 catttttttc ttttttcagt gctcctattt tatctgcaat tttcattctg cttcccaaag
     1741 tttccaataa ctttcgatca aaatcgtcaa tttgggttcg caactgattc attcgttgat
     1801 taaattcatc ggcttcatca gttgtttttc gaacttttaa ctctttaaaa atttgtttta
     1861 aaacatttgg tgtcacttgt tgagcagcat cactccaagc attatcagga tcaatatgtg
     1921 tttcaatgat taatccgtcg taatttaaat cgagtgcctg ctgtgatact tccagtatca
     1981 tatctcgacg accggtaatg tgagaaggat cacaaatcat tggcaaatca ggaaatttat
     2041 tttgcaaatc gatagctatt tgccattctg gattattgcg gtattttgtt ttttcatagg
     2101 tagaaaaacc tctgtgaatt actccaagtt tttgaatatt cgcttggtat aaccgctcca
     2161 aaccaccaat ccataaagac aaatcaggat ttacagggtt ttttattaac acaatcttat
     2221 ccgtattttt taatgcatcc gctatttctt gaacagcaaa tggatttaca gttgttcgtg
     2281 ctccaatcca caacacatca atatcatgtt ctaatgctaa ctgcacatga ttggcattag
     2341 ccacttcaat agccatgagt aatccggttt cagcttttgc tttttgcaac cattttaaac
     2401 caatggctcc tactccttca aatccgccgg gtctggttcg tggtttccag attcccgctc
     2461 taaaaatact gacatctgaa ttttttaatt catgagctat ttttaatact tggtcttcgg
     2521 tttcagcact acatggaccg gcaatcacta atggatgtga taattcaaag tcgtttaacc
     2581 aagttcttaa ttcaatttta ttttccataa ttaaaattct gatgtaaaaa taaggtgttt
     2641 ttttgattcc tattcaatta atctattgcg tttgatttcc tcttttatac tctcctaaaa
     2701 ccttaaaatg agtagtcata atttccaata atttttttgc tttttcatag tgcttgtatt
     2761 tttcaaaaac agcatccaca aaaaaagaat attgaaaagg actttcgata attggcattg
     2821 attggatttt ggttaagttc aatttacaat tattcatcac atttaaaacc gttgctaaac
     2881 ttccgggagt atcatctaat tcaaatttta ttgacgcttt attgatttct tcttttggta
     2941 attctttatt ttttgttttc aacactacaa atcgtgtttt gttgcttttg acagtatgaa
     3001 tagctgaaaa caaggtttcc agttcataca attgagctgc aaccggactt gccaaagcag
     3061 ctatgccttt taaattattc tgttttatgt tttgtgccgc agccgcagta tcactacttt
     3121 ctactaattt aatagttgga tattgactca aaaaaatgcc acattgcaac aaagctatgg
     3181 gatgcgaatg aatttctttt atatcatcta tagtttgtcc ctttaagacc ataaaatcca
     3241 attgaatatc taaataatgc tcgccaatga tatgaagatt atttcgatcg attaaagcat
     3301 aatttgggat aattgaacct gcaatagagt tttctaatgc cataattcca ttatttgctt
     3361 tactttctac catagatttc acaacggatt caaaagaatt acattccaaa atagtacctt
     3421 tctttttaaa atattcttga gccacctgat ggtgaaacga ccctttaatt ccctgaattg
     3481 caattgtaat atccatattt tgagcataaa aaaatcccga ctcaatgaat cgggatttat
     3541 attgttttga attaaaaaat ttaccttaca taacacaata
//
LOCUS       DAOTCW010000196         3522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_21397_length_3522_cov_48.081627, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000196 DAOTCW010000000
VERSION     DAOTCW010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21397_length_3522_cov_48.081627"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1630)
                     /locus_tag="P5335_11200"
     CDS             complement(14..1630)
                     /locus_tag="P5335_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRZ75491.1"
                     /translation="MAEIITMPRLSDTMTEGVVATWLKKVGDTIKEGDILAEIETDKA
                     TMEFESFHSGTLLHIGIQEGQSAPVDSLLAIIGKEGEDISGLLAGGSSPKIEESKSEA
                     PNIETKPQVATAIPAGVIVVTMPRLSDTMTEGTVATWLKKIGDTVKEGDILAEIETDK
                     ATMEFESFNAGTLLYIGIQEGQSAPVDSVLAIIGPTGTDVTGIAENYTKGGSDVSKTE
                     KAEVTKETQPESKQETETTTNGGRIFASPLARQIAKDKGINLSQVKGTGENGRIVKND
                     VENFTLSTTPVTPASTTETFTSTTQPAIQPFVPAGESFSEEAKNSQMRKTIARRLSES
                     KFTAPHYYLTIEVAMDDAMKSRATINSIPDTKVSFNDMVVKACAMALKKHPRVNSQWK
                     EEATVINYHVNIGVAVAVEDGLVVPVLKFTDQMSLTQIGSAVKDLAGRAKNKKLQPAE
                     MEGSTFTVSNLGMFGITEFTSIINQPNSAILSVGAIIEKPVVRNGQIVVGNTMKVTLA
                     CDHRTVDGATGSEFLQTLRTYLENPVTMLA"
     gene            complement(1636..2634)
                     /gene="pdhA"
                     /locus_tag="P5335_11205"
     CDS             complement(1636..2634)
                     /gene="pdhA"
                     /locus_tag="P5335_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRZ75492.1"
                     /translation="MKKITKEVYLQWYEDMQFWRKFEDKLAAVYIQQKVRGFLHLYNG
                     QEAVLAGAIHAMDLSKDKMITAYRNHVQPIAMGVDPKKVMAELYGKVTGTSKGMGGSM
                     HIFSKEHGFFGGHGIVGAQIPVGAGMAFADKYFNTGGVTLTYFGDGAARQGSLHEAFN
                     MAMLWKLPVVFICENNGYAMGTSVERTANHTDVWKLGLGYEMPCGPVDGMNPVKVAEA
                     MHEAIERARRGDGPTFLEMKTYRYRGHSMSDAQLYRSKDEVEEYKKIDPITQVLDIIK
                     ENNYATEAEIEAIDERVKHLVEECEKFADESPYPEIQQMYDVVYDQENYPFISHKL"
     gene            complement(2811..3293)
                     /locus_tag="P5335_11210"
     CDS             complement(2811..3293)
                     /locus_tag="P5335_11210"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRZ75493.1"
                     /translation="MKKITITTEFSVFDNKEQLPEEIKNLMELAIEIRKKAYAPYSKF
                     RVGAAILLDNGKIVLGSNQENAAYPSGLCAERVAIFQAGAIYPDAIILKLAITATADE
                     KPVNSPIPPCGSCRQSIAEYEFKQNSPIEIFFMGESGEVYKSDSLTNLLPLVFDKNYL
                     "
BASE COUNT          996 a          669 c          595 g         1262 t
ORIGIN      
        1 tattcggcta aatctatgcc aacatagtca ccggattttc taaataagtt cgcaacgttt
       61 gtaaaaattc agatcctgtt gcaccatcaa ctgttctatg gtcacatgct aaggtaactt
      121 tcatggtgtt gcccacaact atttgtccgt ttcttaccac cggtttttcg atgattgcac
      181 caactgaaag aattgcagag tttggttggt tgatgattga agtaaattca gtaataccaa
      241 acattcctaa gttagaaact gtgaaagtac taccttccat ttccgcaggt tgaagttttt
      301 tgttttttgc tcttcctgct aaatctttca cggctgaacc aatttgagtt aaactcattt
      361 ggtctgtgaa tttcaaaaca gggacaacta aaccgtcttc aacagctact gcaacgccaa
      421 tgtttacatg ataattaatg acagttgctt cttccttcca ttgagaattt actcttggat
      481 gttttttcaa cgccattgca caagctttta cgaccatatc gttaaacgat actttcgtgt
      541 caggaattga atttatagtt gctcttgatt tcattgcatc gtccatagca acttcaattg
      601 ttaagtagta atgtggagcc gtaaatttag attcagaaag acgacgagca atagttttac
      661 gcatctgtga atttttcgct tcttctgaaa aagattcgcc agcaggaaca aatggttgaa
      721 ttgcaggttg tgtagttgaa gtaaaagttt ccgttgtaga agctggagtt actggtgtag
      781 ttgaaagtgt aaagttttct acatcatttt ttacaattct gccattttct cctgttcctt
      841 tcacttggga aaggtttatc cctttgtctt tagcaatttg tcgggctaat ggtgaagcaa
      901 aaattctacc accgtttgtt gttgtttcag tttcttgttt tgattcaggc tgtgtttctt
      961 tagttacttc agctttttca gttttagaaa catcactacc acctttggta tagttttctg
     1021 caattccggt gacatctgtt cctgttggtc cgataatagc taatacacta tcgaccggag
     1081 ccgattgtcc ttcttgaata cctatgtaca atagagtgcc ggcattgaaa gactcaaatt
     1141 ccatggtggc tttgtcagtt tctatttcgg caagtatgtc tccttctttt acagtatcgc
     1201 ctatcttttt taaccaagta gcaactgttc cttcagtcat cgtgtcactt aaacgaggca
     1261 tcgtaacaac aattacgccc gcaggaatag ctgttgcaac ttgtggttta gtttcaatat
     1321 ttggtgcttc tgattttgat tcttcaattt ttggtgaact tcctccagcc aataaacctg
     1381 aaatatcttc cccttcttta ccaataattg ctaaaagtga atcaactgga gccgattgtc
     1441 cttcttgaat tcctatatgt aataaggttc ctgaatgaaa cgattcaaac tccattgttg
     1501 ctttatccgt ttcaatttct gctaaaatat caccttcttt tattgtgtcc ccaacttttt
     1561 taagccaagt agctacaact ccttcggtca ttgtgtcact caaacgtggc attgttataa
     1621 tttctgccat agctgttata gtttatgaga tataaatgga taattttctt ggtcatatac
     1681 tacatcatac atttgttgta tttcaggata tggtgattca tcagcgaatt tttcacactc
     1741 ttcaactaag tgttttactc gttcatcaat tgcttcaatt tctgcttctg ttgcataatt
     1801 gttttctttg ataatatcta aaacctgagt aattgggtca atttttttat actcttcaac
     1861 ttcatcttta cttctgtata attgagcatc agacatggag tgacctctgt atcggtatgt
     1921 tttcatttct aagaaagtag gtccatcacc tctacgggct ctttctatag cttcgtgcat
     1981 ggcttcagcc acttttacag gattcattcc gtcaactggt ccacacggca tttcatagcc
     2041 taaaccaagt ttccaaacat cggtatgatt agcagttctt tcaacagaag ttcccattgc
     2101 gtaaccattg ttttcacaaa taaaaactac aggaagtttc cataacatgg ccatattaaa
     2161 agcttcatgt aaagaacctt gacgagcagc accatcacca aaataggtta aagttacacc
     2221 cccggtgtta aaatatttat ctgcaaacgc cattccagca cctacaggaa tttgagcacc
     2281 tacaattcca tgtcctccaa agaaaccatg ctctttagag aaaatgtgca tagatccacc
     2341 cattccttta gaagttccgg ttactttacc gtaaagttca gccattactt ttttcgggtc
     2401 aacacccatt gcaattggtt gaacatggtt tctgtaggct gtaatcattt tatctttaga
     2461 taagtccatt gcatgtatag cacctgccaa aacagcttct tgaccgttat ataaatgtag
     2521 aaatcctcga actttttgtt gaatgtaaac ggctgctaat ttatcttcaa attttctcca
     2581 aaactgcatg tcttcatacc attgcaggta aacttctttg gtgatttttt tcattttttt
     2641 atttcttgaa tctttttgtt tgaattgttt ttttgcaagt atattgtata ttataaaatt
     2701 gcgaatagca aaaataagac atccttagta gatgtaaaaa tttaatcgac ttaaaatgat
     2761 aaactttata aaaagttatt tacgataacg ttatcgttat gaaattttat ttataaatag
     2821 tttttgtcga aaactaatgg caataaattg gtaagtgaat cagatttgta aacctctccg
     2881 gattctccca tgaaaaatat ttcaatcggt gagttttgtt taaactcgta ttcggcaatt
     2941 gattgacgac aagagccgca gggcggaatg ggtgagttaa ccggtttttc atcggcagtt
     3001 gcggttattg ctaatttcaa aattatggca tccggataga tagctcctgc ctgaaagata
     3061 gcaacacgtt ctgcacaaag cccggaaggg taggcggcat tttcctgatt agaacctaaa
     3121 acaatttttc cattgtcaag taaaatggca gcaccaactc tgaatttgga gtaaggagcg
     3181 tatgcttttt ttctgatttc aatagctaac tccattagat tttttatttc ttctggaagt
     3241 tgttccttgt tgtcaaaaac tgaaaattca gttgtaatag taattttttt cataaagtaa
     3301 gggggaaaaa aaatccaaat ctcgaattag aaatttggat taatttttta ttattttttc
     3361 attctagtat tcatcatatt tatctccaaa attaaacgtc agtgtaaaac gtaacgtgtt
     3421 ttctagtggg tttttaacat ttgaagcaga cattaaatag gatacatcaa tttttactac
     3481 attgtattta aatccggcac caaaagagaa aaattgtctt gc
//
LOCUS       DAOTCW010000197         3514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_21467_length_3514_cov_42.545533, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000197 DAOTCW010000000
VERSION     DAOTCW010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21467_length_3514_cov_42.545533"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..3368)
                     /locus_tag="P5335_11215"
     CDS             complement(33..3368)
                     /locus_tag="P5335_11215"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ75494.1"
                     /translation="MKKQFLLVLSLVFILSISCSEKKTNIQGKENSEKTITSSEIKTT
                     AAENEDLNNENTKISSKTDQIQVASVSKKELIVDEEIEMLRKKHENFLKNSPFKKVML
                     LPKKERKAIGIPPNKYYEQEWELTMNPETGKPDIAKLIKLRQQLIQERDSLLLSGRTP
                     GDGSDNNWIERGPNNVGGRVRAVMFDPNDATNETVFAGGVSGGLWKNTTISNPSSTWT
                     RVNIPDNLAVSTITYDPNNTNIFYVGTGESYVGGDVNGDGVWKSADGGNTWSKVFGGI
                     SGPLNFVSASDVTINSPALIAGNYPSYPTNPANFGTEITSPITADIVLIADNTAPTSD
                     GCQTITNGAAISGKIALIRRGTCTFVLKVKAAQDAGAIGVIMMNNIDGTPIPMGGTDA
                     TIVIPSVMISKADGDILEAALSSGPVNGTLNPSTGSFTGNVLPGQQHINDIKVRNNGG
                     VSEIYVAAGDSFYGSANATTYLGGPDFGLYKSIDGGATFTEISLPLTATGNKHCPNNI
                     EIGSDNKIWLSTINSVVFGDGGGKIFSSTDGNTFVDEYTISGGDRTQIAVSNQTADKV
                     YVLAELTEGGVTMIGTTNGFTTTSTLALPIDADSGIPANDFCRGQAFYDLMLEVDPTN
                     DQILYTGGIDLFKSSNGGTSWSQFSHWYGGFGYQDVHADQHGLAFGHGASNKLLIGND
                     GGVYFSANGGTTTTARNLGLNITQFYSVGVSPTAAVSGLTGDNYFAAGAQDNGTQYFG
                     NASADINASVRSQGGDGAFTMFDQGADKYYISNYVYNSNINLRPIPSGTVRTLSNDAS
                     SDTNGAFIAPMVLDSNQDVLYSDYSTTTTNQIRRYKNIKSGIINRVNLTNALLTSRPT
                     AFAVSPYTTTSTTLLVGTRLGKLLKITAANSIALWSDITGPEFVGSISDVEYGSSEND
                     IFVTMHNYNVVSVWYSADAGVTWQNKEGNLPDLPVKCILQNPLNANEVIIGTELGVWF
                     TNNFSAATPTWNQSYNGMSNVKVTDLDLRNDNTVFASTYGRGLFSGTFTATSLSSNDV
                     TLNKGIKLYPNPSNGIVTISIENFSGNLNVAVYDLNGRKVLSNSIDFSTQTSINLEGL
                     QSGVYVLKLEGDNLSYSEKIILQ"
BASE COUNT         1106 a          671 c          533 g         1204 t
ORIGIN      
        1 gctatttagg ggtttttaat agtcaacttt tcttattgta aaataatttt ttcagaataa
       61 gataaattat cgccttctaa tttcaataca taaacaccag attgtaaacc ttcgagatta
      121 atactcgttt gtgttgaaaa gtcaatagaa ttagataaaa cttttcgtcc atttaagtcg
      181 taaactgcta cattcaaatt acctgaaaag ttttcaattg aaatagtgac aatgccatta
      241 gatggatttg gataaagttt tatcccttta tttagagtca catcatttga agaaagtgat
      301 gttgcggtaa aagttccgga gaaaagacct cttccgtaag ttgacgcaaa aacagtatta
      361 tcatttctta aatctaaatc cgtaactttc acattactca ttccattata ggattgattc
      421 caagtagggg ttgcagcact aaaattatta gtaaaccaaa ctcctaactc agttcctata
      481 ataacttcgt tagcattaag tggattttgt aaaatacact taacaggtag atctggtaaa
      541 ttaccttctt tgttttgcca tgttacaccg gcatcagcac tataccaaac actaaccaca
      601 ttataattgt gcattgttac aaaaatatca ttttctgaag aaccatattc aacatctgat
      661 atactaccta caaattcagg acctgtaata tcagaccaca atgcaatgct attagctgct
      721 gtaattttta aaagctttcc taatcttgtt ccaaccaata gagttgtgga agtagttgta
      781 tagggagata cagcaaaggc agttggtcgt gaggttaata atgcatttgt taaattaact
      841 cggtttataa tacccgattt aatgttttta tatcttctaa tctgattagt tgttgttgta
      901 gaataatcag aataaagaac atcttgatta gaatctaata ccattggagc tataaaagca
      961 ccatttgtat cacttgaggc atcattgctc aatgttctga ctgtaccgga tggaattggt
     1021 cttaaattga tattactatt gtaaacataa ttagaaatat aatatttatc agcaccttga
     1081 tcaaacattg taaacgctcc atctcctcct tgtgacctaa cacttgcgtt aatatccgca
     1141 gaagcattac caaagtattg tgtgccatta tcttgagcac cggcagcgaa gtaattatca
     1201 ccggttaatc cactcacagc agcagttggt gaaacaccta cactataaaa ttgtgtaata
     1261 tttaaaccga gatttctggc agtagttgtt gtacctccat tagcagaaaa atataccccg
     1321 ccatcgttac caatcaataa tttatttgat gctccatggc caaaagctaa accgtgttga
     1381 tctgcgtgta cgtcttggta accaaaacca ccataccaat gcgaaaactg actccaggaa
     1441 gttccaccgt ttgaggattt aaataaatct ataccaccgg tatataaaat ttgatcgtta
     1501 gtaggatcca cttctaacat taaatcatag aaagcttgac ctctacaaaa atcgtttgca
     1561 ggaattcctg aatcagcatc aataggtaaa gctagggtag atgtagtggt aaatccgtta
     1621 gtagtaccaa tcatggttac tcctccttca gttaattctg ctaaaacata gactttatct
     1681 gcagtttgat tagatactgc aatctgtgtt ctatctcccc cggatatcgt atactcatca
     1741 acaaatgtat ttccatcagt agaagaaaat atttttcctc ctccatctcc aaaaaccaca
     1801 ctatttatag ttgataacca aattttattg tcggaaccaa tttcaatatt gtttggacaa
     1861 tgtttgtttc cggtagcggt taacggtaat gaaatttctg taaaagtagc accaccatca
     1921 attgatttat ataaaccaaa atctggacct cctaaatagg tagttgcatt tgcagagcca
     1981 tagaatgaat ctccggcggc aacatatatt tcagaaactc caccattatt tcttactttt
     2041 atatcattta tatgttgttg accaggcaaa acatttcctg taaaacttcc tgtacttgga
     2101 tttaaagttc cattcactgg tcctgaactc aaagcagctt ctaaaatatc tccatcagct
     2161 ttagaaatca ttacagaggg gataactata gtagcatctg ttccacccat tggaattggt
     2221 gtgccatcaa tattattcat cataataaca cctatagcac ctgcgtcttg tgccgcttta
     2281 acctttaata caaacgtaca agtccctctt cttattaatg caattttgcc actaattgcc
     2341 gcaccatttg ttattgtttg acatccatca cttgttggag ctgtattatc agcgattaaa
     2401 acaatatctg cagtaattgg agaggtgatt tctgttccaa aattagctgg atttgtagga
     2461 taggaaggat aattacctgc aatcaatgct ggactattaa tagtaacatc tgatgctgaa
     2521 acaaaattta atggtccgga aatcccacca aaaaccttac tccatgtatt tccaccatct
     2581 gcagacttcc aaacaccatc accgtttaca tcacctccca cataagactc tcctgtgcca
     2641 acgtagaata tgtttgtgtt gttagggtca taagtaatcg ttgaaacggc taagttatca
     2701 ggaatattaa cgcgagtcca agttgaagag ggattagaaa tagtagtgtt tttccacaaa
     2761 cctccactaa ctcctcctgc aaaaaccgtc tcatttgtag catcattagg atcaaacata
     2821 acagctctaa ctctaccgcc cacattatta ggacctcttt ctatccaatt attatcagag
     2881 ccgtcacctg gcgttctgcc tgaaagtaat aaactatcac gctcttgtat taattgttgt
     2941 cttagtttaa ttaatttagc tatgtctggt tttcctgttt caggattcat tgtcaattcc
     3001 cattcctgtt cataatactt attaggcgga attccaatag cctttctctc ttttttaggg
     3061 agaagcatta cttttttaaa aggactgttt ttaagaaaat tttcgtgttt ttttctcaac
     3121 atttcaattt cttcatctac aattagttct ttttttgata cagaagctac ttgaatttga
     3181 tctgttttgc tgctaatttt agtattttca ttattcaaat cttcattttc tgctgctgtt
     3241 gtttttattt ccgaagaagt aatagttttt tcactattct cttttccttg aatattggtt
     3301 ttcttctcag aacaactaat acttaaaata aaaacaagac ttaatactaa aaggaattgt
     3361 tttttcatgg ttattttcgg ttaaatttgt tttaattcta acaaaattag tactttatat
     3421 caattttcaa atttttttat tgtttttaaa ttaaaatagt acgaattagg acaaaaacac
     3481 cctacaattg ctagagtttc gtagggcttg caaa
//
LOCUS       DAOTCW010000198         3457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_21986_length_3457_cov_34.133157, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000198 DAOTCW010000000
VERSION     DAOTCW010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21986_length_3457_cov_34.133157"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="P5335_11220"
     CDS             <1..527
                     /locus_tag="P5335_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75495.1"
                     /translation="ATSIAGIGFWAEAAAFIPDEDVIMTNDLSAFYPLSPVPVTQDSL
                     ILDKTKPYIKFVVGGDYNFADGSYLNLQYMHGFIHERGAENLNDYFFLRYEKKFFNEK
                     LKVAPIGGGFIVTDWNNIKDNYALVYMPEIAYQATINSEITLATTIFDGKGDNLFSNL
                     KDYNMFMLKLKYSF"
     gene            1026..2039
                     /locus_tag="P5335_11225"
     CDS             1026..2039
                     /locus_tag="P5335_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75496.1"
                     /translation="MENGMIEINNLFATHQTDIEKISLDNVIEIAEKYKINLKKKFKA
                     NRLELFSKYLHHCLIDSKLEDSELIALKHVKDLMLLSDKETQELLKAETEKLYSQHVK
                     EAVSDGRLEEFEKEKLEKLKKDLLISEEIADRIYESNAKQILQNFIDGVISDGRLSPD
                     EETEITEIAKSLNIDLKVDDKSKEVLDRYKLYWQIENGELPTIVSDINIQKSENLHFK
                     ASVKWLEQRRVTKRVNYGGPTARIKIAKGVYYRMGSVGVQRVSEDVWQTIDSGQLYLT
                     NKRLIFMGGKGNKTIQLNKVLDVTPYKNGVDIQKESGKSPFLEFNQNVDIFSMILVRL
                     MDE"
     gene            2219..3007
                     /locus_tag="P5335_11230"
     CDS             2219..3007
                     /locus_tag="P5335_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75497.1"
                     /translation="MKNVLILIFLSGIIFKTYSQEEQLFSEYKEYLIDSTEIKLLGNY
                     GYFFSGQFEIIDSSYFHNIFGEENSLPLNHAYQYGGHLGINRNHLYIGLNCYFNWSKS
                     SSDTISIKLNQTAYSINLGYNIIINRKILITPFLGIKYNKYRYRKSNFNNQITIDDYL
                     KNPGYDLKLHEFSLPIGLNMNFNYRNWFSYGLQVSYINPFTHVSYLYSDNNRINNGNK
                     KALGNVSFLIGFGIGVNHLYRIFKFGNNIDDNVGIYRKRIDENK"
     gene            complement(3277..>3457)
                     /locus_tag="P5335_11235"
     CDS             complement(3277..>3457)
                     /locus_tag="P5335_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75498.1"
                     /translation="LSVHFLSWHCLFLALPTTPCAEAGPGTHLTIFPEAGGLAFAQRY
                     GLATFISTFLMLPRL"
BASE COUNT         1252 a          518 c          642 g         1045 t
ORIGIN      
        1 tggctacaag cattgcaggt attggttttt gggccgaggc agcagcattt attcccgatg
       61 aagacgtaat catgaccaat gatttgtctg ctttttatcc gctgtcaccc gttccagtaa
      121 ctcaggattc attaatttta gacaaaacaa agccttacat taaatttgtt gttggtggcg
      181 attacaattt tgcggatggt tcgtatctta atcttcaata catgcacggg tttattcacg
      241 aacgaggagc cgaaaacctg aatgactatt tctttttacg gtacgaaaag aaatttttca
      301 atgaaaagtt aaaagtagct cctattggtg gtggtttcat agttacagac tggaataata
      361 taaaagataa ttatgccctt gtgtatatgc ctgagattgc ttaccaagcc actattaatt
      421 cagaaatcac tttagcaacc acaatttttg atggcaaagg agataatttg ttttcgaatc
      481 tgaaggatta caatatgttt atgttaaaat tgaaatatag tttttaatgt taaatcaaat
      541 atgccctaca cactgccgta agcacatttg caaccccgca cgagccaacg ctccaaccaa
      601 aggcttgcaa aagagcttcc gcctctccag cccaagcaac cgcctttcag gacggtttta
      661 ggattgtcca cgctcaaaaa aacaaaataa atttgtgctt cgttgatagg ttggtgcaga
      721 ttgataatga caagaaaata aagctaaaca aatgacatac agacagatat gaatgaacac
      781 cagttgccaa catcgtatat aaaacatttg gcagtcagtg cgttattaag catttcagct
      841 cgtattatag gcacttgtaa cttgacagga aagtgcttcg aaatgccaaa cgtttcatat
      901 acgtgaccgt tatgtgccaa attaaaaaaa acgtcgtacc ttaaaaaata actaatatgg
      961 aaatttataa cccgcaagaa ttaaagaaac ccaatttgat tcaaagattg tttggaaaaa
     1021 ttccaatgga aaacggaatg attgaaataa acaatttatt tgccacacat cagacagata
     1081 ttgaaaagat ttcgcttgac aacgtaattg aaatagcgga aaaatataag attaacttga
     1141 aaaagaaatt taaggccaat cgccttgaat tgttttcgaa atatcttcat cattgcttaa
     1201 ttgatagcaa attagaagat tctgaattaa ttgcactaaa acatgttaaa gacttaatgt
     1261 tacttagtga caaagaaaca caagaacttt taaaagctga gactgaaaaa ctatatagcc
     1321 aacatgttaa agaagctgtt tcagatggaa gattagaaga atttgaaaag gaaaaattag
     1381 agaaacttaa aaaggattta cttatctcag aagaaatagc ggacagaatt tatgagtcca
     1441 atgcgaaaca gattttgcaa aactttattg acggggtgat ttctgatgga cgtttgtctc
     1501 ccgatgaaga aactgagata actgaaattg ctaaaagcct aaatattgat ttgaaagttg
     1561 atgacaaatc aaaagaagta cttgatagat acaaattgta ttggcaaatt gagaatggcg
     1621 aattgccaac gattgtttct gatattaata ttcaaaaatc agaaaatctt catttcaagg
     1681 cttcagtaaa atggcttgag caacgtagag ttacaaaaag agtaaattat ggtggaccaa
     1741 ctgcaagaat taaaattgca aaaggagttt attaccgaat gggtagtgtt ggtgttcaaa
     1801 gagtttcaga agatgtttgg caaacaattg actcaggaca attatacttg actaataaga
     1861 gattaatctt tatgggtgga aaaggtaata aaacgattca gttgaataaa gttttagatg
     1921 taactcctta taagaatggg gttgacattc aaaaagaatc tgggaaaagt ccttttttgg
     1981 aatttaatca gaacgttgac attttctcaa tgattttagt aagattgatg gatgaataaa
     2041 aattcggcac ataacacgcg gtataaaaaa ttgccgggac attaggttct tcaagggttg
     2101 tagcccgctt caacttttgt gtaacttgac aaggaatcac ccgcaatcgg caacttttca
     2161 taccgccacc gttaggcaaa actatggaag gctctctgaa taattaagga atacatatat
     2221 gaagaatgtt ttaattttaa tatttttatc aggaataatc ttcaaaacat atagccaaga
     2281 agaacaatta ttttctgaat acaaagaata cctgatagat tctactgaga ttaaattgct
     2341 tggaaattat ggttattttt tttctggtca atttgaaata atagactctt catattttca
     2401 taatattttt ggagaagaaa attctcttcc attaaatcat gcttaccaat atggcggaca
     2461 tttgggaata aacaggaatc atttatacat agggttgaat tgttatttta attggagtaa
     2521 atcatcttca gatacgatat caattaaact gaatcaaact gcttattcta ttaatcttgg
     2581 ttataatatt attattaacc gaaaaatatt gataacgcct tttttaggaa tcaaatacaa
     2641 taaatatcgt tatagaaagt ccaactttaa taatcaaata acaattgacg attaccttaa
     2701 aaatcctggc tatgatttga agttgcatga attttcttta cctattggac taaatatgaa
     2761 ttttaattat agaaattggt ttagttatgg attacaagta tcttatatca atccattcac
     2821 gcatgtttca tacctgtatt cagataataa cagaataaat aatggtaata aaaaagcatt
     2881 gggtaatgtg agttttctga ttggttttgg aataggagtt aatcatttat atagaatatt
     2941 caaattcgga aataatattg atgataatgt tggtatttac agaaaacgaa ttgatgaaaa
     3001 taaatagctt tgcctaacaa taaatatacg taatgcgggg tgatttaata aatttgagct
     3061 tttaagcatt taataaacaa tgtagcggtt tgatagcgaa gcgccttgga aacccgcact
     3121 acgcatattc gagccgttat gggcaagcat aatagaaaaa aatggatgat caaaagacaa
     3181 tcggatctgc aagctcagga catttggcct tgattctacc gtatctatga taaaaaaaag
     3241 ttggagtccg gaagagcagg tggttgttta tgagcattat aaccttggaa gcattaaaaa
     3301 agtacttatg aaagtggcca gcccataacg ttgtgcaaat gccagcccac cggcctctgg
     3361 gaaaatagtg aggtgtgtac cggggccggc ttctgcacat ggcgttgtgg gtaatgctaa
     3421 aaaaagacag tgccatgaaa gaaaatgaac agaaaga
//
LOCUS       DAOTCW010000199         3393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_22565_length_3393_cov_48.466147, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000199 DAOTCW010000000
VERSION     DAOTCW010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22565_length_3393_cov_48.466147"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="P5335_11240"
     CDS             <1..1185
                     /locus_tag="P5335_11240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRZ75499.1"
                     /translation="KLKENDFTDLPGSFYTALKIRDSLKITTGIIVNKIHISEYYDAK
                     KQKDSALKYANEAHSLALSSNSIGHQLLTLKQLGNVETEKSNFYSKEYVSLSDTLIQT
                     ERKVSEKFARIEFETDEFKRQKEQLTIQNRNIILFALLAFFIVVLLYIIRDQRSRNAR
                     FKLREAQQRANEEIYALMLSQQKKLDEVVQKEKQRIGQELHDGVLGRLFGARLNLDSL
                     NKKDDEQAVAGRNHYISELKNIEQDIREISHELNREKFALINNFVAILTNLFEEQKTL
                     SEDTDLKYSIDSAIKWDKVGNNIKINVYRIIQESLQNINKYAAAKNISINLIKDADNL
                     HINIIDDGVGFDVDKKSKGIGLQNIESRVTSCHGTFELKSKKGKGTSIAIDIPLEQQP
                     TT"
     gene            1188..1853
                     /locus_tag="P5335_11245"
     CDS             1188..1853
                     /locus_tag="P5335_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRZ75500.1"
                     /translation="MSKNYQIIIVDDHPFIIEAYKNALKSYSANNFDYTTTQANNCKS
                     AYEIITTASQPFDIAFLDISMPPYEEKNIMSGEDLAKLLRKVMPDCKIILLTMHTELI
                     KINSIIKEINPNGLIIKNDLTFDELLNAFNKILNNDNYYSQTVVKFVSQSQYENLQVD
                     EFDKQILFHLSKGVKTKDLPSYVPLSLSAIEKRKLNLKEILSVKGSSDIELITEAKNK
                     GLI"
     assembly_gap    2016..2115
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2368..>3393)
                     /gene="thiL"
                     /locus_tag="P5335_11250"
     CDS             complement(2368..>3393)
                     /gene="thiL"
                     /locus_tag="P5335_11250"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRZ75501.1"
                     /translation="QRTSIAQLGEFGLIDHLTKNFDIKNPSTLKGIGDDAAVLDFKDK
                     KVIVSTDLLIEGVHFDLAYMPLRHLGYKAVVANVSDICAMNAKPTQITVSIAVSSRFP
                     LEALEELFDGITIASQIYNVDVVGGDTTSSQKGLIISITAIGEAKEEEIIYRNGAKEN
                     DLVVVTGDIGAAYMGLQVLEREKQVFQVNPNNQPDLENYTYLIERQLKPEARTDIREL
                     LEKLEVKPTSMIDISDGLSSEVMHLCKQSIVGCTIYEEKLPLDPQLISVCEEFNIDST
                     TVAINGGEDYELLFTIALEDFDKIKANPNLTVIGHINKQSEGIHLVTRANQKIEMKAR
                     GWNALEE"
BASE COUNT         1193 a          530 c          530 g         1040 t
ORIGIN      
        1 aagttaaaag agaatgattt tacagattta cccggttctt tttatacagc tttgaagatt
       61 agagacagtt tgaaaattac aacaggaatc attgttaaca aaattcacat atctgaatat
      121 tatgatgcta aaaagcaaaa agacagtgcg ttaaaatatg ccaatgaagc acatagtctt
      181 gcattatctt caaatagtat tggacatcag ttgttaactt taaagcaatt aggaaatgtt
      241 gaaactgaaa aaagtaattt ttattcaaaa gaatatgtta gtttaagtga tactttaata
      301 caaactgaaa gaaaggttag tgaaaaattt gcccgaattg aatttgaaac agatgaattc
      361 aagcgacaaa aagaacaatt aacaattcaa aatcgaaata ttattctttt tgctttatta
      421 gcatttttta ttgttgtttt gctttacatt attcgagatc aacgaagtag aaatgcccgt
      481 tttaaattac gtgaagctca acaacgtgct aatgaggaaa tatatgcttt gatgttgagt
      541 caacaaaaaa aattagatga agttgttcaa aaagagaaac agcgaatcgg ccaggagtta
      601 catgatggag ttctagggcg tttgtttggg gcgaggttga atttggacag tttaaataaa
      661 aaagacgatg agcaggcagt tgccggaaga aatcattata tttcagaatt aaaaaatatt
      721 gaacaggata ttcgagagat ttctcatgaa ttgaatagag agaaatttgc cttgattaat
      781 aactttgtgg ccatcttaac taatttattt gaagaacaga aaacattatc tgaagatact
      841 gatttaaagt acagcattga ttcagctatt aagtgggaca aagtgggtaa caatattaaa
      901 attaatgttt accgcatcat tcaagagtcg ttacaaaata ttaataagta tgcagcggct
      961 aaaaatattt ctattaattt gattaaagat gccgataacc tgcacatcaa tataattgat
     1021 gatggtgttg gttttgatgt tgataaaaaa agtaaaggaa ttggtttgca aaatatagaa
     1081 tctcgcgtaa cttcgtgcca cggaacattt gaattaaaat caaaaaaagg aaaaggaaca
     1141 agtattgcca ttgatatacc attagaacaa caacccacta catagccatg tcaaaaaatt
     1201 accaaattat tattgttgat gatcatcctt tcatcattga ggcctacaaa aatgctttaa
     1261 aaagctacag tgctaataac tttgattata caaccactca ggccaataat tgtaaatcag
     1321 cttatgaaat tattacgacg gcatcacaac cgtttgatat tgcatttcta gatataagta
     1381 tgcctcctta tgaagaaaaa aacattatgt caggagagga tttggctaaa ttgcttcgta
     1441 aggtgatgcc tgattgtaaa atcatcttgt tgaccatgca caccgagtta atcaaaatca
     1501 attcaattat taaagagatt aatccaaatg gactaattat taaaaacgat ttgacctttg
     1561 atgaattatt gaatgcattt aataaaattt taaataatga taattattac agtcaaacgg
     1621 tagtaaagtt tgtgagtcaa tctcaatacg aaaatctaca ggttgatgaa tttgataaac
     1681 aaatattatt tcatttatct aaaggagtta aaaccaaaga tcttcccagt tatgtcccac
     1741 tttcattaag tgccattgaa aagcgaaaat taaatttgaa agaaattctt agcgtaaagg
     1801 gaagtagtga tatagagctt attacagaag caaaaaacaa ggggttgatt taatcacccc
     1861 ttttttattg gaattaaccg tagtaaaatt ggcgtaaaac cacatccttt tttgtgggaa
     1921 accgtaatta aagtattgaa aatcaatata cttttacatc gagttagtta gcatactaga
     1981 atttacaaat caacctaaga acaaaacaga gtaacnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnacatt ctgcggagaa ccgtatttga aaatgaaaag caccaggcta
     2161 tctttacatc atcaataacg aagtaattat cataataaaa atattgacct aagaacgtaa
     2221 ctaagtagtc gaacccgaag aagaaatacc acccttttgc cccaacgaaa gatcttattc
     2281 aaaccaaaga caactactta aaaagtcaaa actaaaaaaa gcgaaacacc ccaatgtctc
     2341 gcttttttta ttttttaaat caaatagtta ttcttccaag gcattccaac ctctagcttt
     2401 catttcgatt ttttgattgg ctcgggtaac taaatgtatt ccttctgatt gtttattgat
     2461 gtgaccaatt acagtaaggt ttgggttcgc ttttatttta tcaaaatctt ctaaagctat
     2521 tgtgaacaat aattcataat cttcccctcc attaatagct acggttgtac tatcaatatt
     2581 gaattcttcg caaacagata ttaactgagg atctaacggt aatttttctt cataaattgt
     2641 acagccaacg atagattgtt tgcaaagatg catcacttct gaagacaatc catctgaaat
     2701 atctatcatt gatgtgggtt ttacttctaa tttttctaat aattcccgaa tatctgtgcg
     2761 agcttccggt ttcaattgcc gttcgattaa atacgtataa ttttctaaat ccggttgatt
     2821 gtttgggttg acctgaaata cttgtttttc gcgttccaaa acttgtaagc ccatataggc
     2881 agcaccaata tcaccggtaa cgacaaccaa atcattttct ttagccccgt ttctatatat
     2941 aatttcttct tctttagctt ctccaatggc ggtaatggaa ataattaatc ctttttgcga
     3001 agaagtagtg tcgccaccca ctacatccac attataaatt tgagaagcaa tcgtaattcc
     3061 atcgaacaat tcttctaaag cttctaaggg aaatctactg gaaacggcaa tcgaaactgt
     3121 aatttgagtc ggcttagcat tcatcgcaca tatgtcagaa acattagcca cgactgcttt
     3181 ataacctaaa tgtcttaatg gcatataggc caaatcaaaa tgcacccctt caattaataa
     3241 atcagtggaa actatcactt ttttatcttt gaaatccaac acagccgcat cgtcgccaat
     3301 tccttttaaa gtagaaggat ttttaatatc aaaatttttg gtcaaatggt caattaaacc
     3361 aaattcgccc aattgagcaa tggatgtgcg ttg
//
LOCUS       DAOTCW010000200         3374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_22759_length_3374_cov_36.193432, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000200 DAOTCW010000000
VERSION     DAOTCW010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22759_length_3374_cov_36.193432"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..1279)
                     /gene="lgt"
                     /locus_tag="P5335_11255"
     CDS             complement(353..1279)
                     /gene="lgt"
                     /locus_tag="P5335_11255"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRZ75502.1"
                     /translation="MVTQFLSFVWNPSEGFEIGSFTVRFYSLMFVIAFGLGWYIMKYI
                     FVRENESLEKLDSLFIYTVIATLIGARLGHVIFYQPELFREDPLSIFLPIRTKPSFEF
                     TGFSGLASHGAAIAIILFMVYFSKKIMHKSLLWVLDRVVIPVASGAVFVRLGNFFNSE
                     IVGHETTSSFGIKFVQDFYSKREAVENTQIANPKEAYKAIAENPQFSNLLEAVPAKHP
                     SQLYEAFFYIFVFLILFFMYWKTDARKKEGLIFGTFMVLLWSVRFGVEYLKESQGGFE
                     TYPLFNALTTGQWLSIPFILIGFYFIFRPKKV"
     gene            complement(1364..1588)
                     /gene="yidD"
                     /locus_tag="P5335_11260"
     CDS             complement(1364..1588)
                     /gene="yidD"
                     /locus_tag="P5335_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRZ75503.1"
                     /translation="MLKKIIRFPFIFLIRFYQVVISPLTPATCRFEPTCSSYSHEAIV
                     KHGVIKGIWLAVKRISKCHPWGKSGYDPVP"
     gene            complement(1626..3107)
                     /gene="cysS"
                     /locus_tag="P5335_11265"
     CDS             complement(1626..3107)
                     /gene="cysS"
                     /locus_tag="P5335_11265"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRZ75504.1"
                     /translation="MKLYENQTLKIYNSLSGEKEVFTPIHQGNVGMYVCGPTVYSNVH
                     LGNVRTFMSFDVIFRYLLHLGYKVRYVRNITDAGHLENDAEEGEDRIAKKARIEQLEP
                     MEIVQRYTVDFHNILHTLNFLPPSIEPTATGHIIEQQEIIKKIIENGFAYEANGSVYF
                     DVVKFNETNHYGKLSGRNIEDMIANTRDLDGVSDKKNPQDFALWKKAEPQHIMRWPSP
                     WGIGFPGWHLECTAMSTKYLGETFDIHGGGMDLKFPHHECEIAQNEACTGHSPVNFWM
                     HANMLILNGKKMAKSTGNYILPNEIFTGENEILSKAFSPNVARFFMLQAHYRSILDFS
                     NEAILGSEKGFNRLMEGMELLKNLSASSSSTLDIFDWKKSCYDAMNDDFNTPILIAQL
                     FEGIRFVNLVNDGKESLTAADIQKLTSTLHAFVYDVLGISDEKSCNDNIDKLEGVVNL
                     MIQMRMEARANKNFALSDQIRDQLLAIGIQLKDGKEGTSFSVN"
     gene            complement(3126..>3374)
                     /locus_tag="P5335_11270"
     CDS             complement(3126..>3374)
                     /locus_tag="P5335_11270"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HRZ75505.1"
                     /translation="VDYFAKRPQVQERLTMQIVQELQKALNTEDVACVIDAKHLCVNS
                     RGIRDIESSTVTSEFGGKFKDDKVRREFLDYIKLETKF"
BASE COUNT         1163 a          613 c          493 g         1105 t
ORIGIN      
        1 gtgcatttaa agaagaagaa aagaaataat tctttatcaa catattagta ttaaaacccg
       61 gctttaagtc gggttttttt atggatatta ttttcaacta tattgaaatt aaaaaacttg
      121 tttgatatct ttataatttg ggatagtggt aaagtaataa taaaagaata acctttattc
      181 cgttttgcag ctgataggtt ttgaaacctg tcagctatga ctaatttatc attcaaaatt
      241 gataatcatc ttcaattttt atttggagtt ctactaatgc tttttgtcac tattaccaat
      301 aaaaaaaacc gactcattaa agtcggttag tttaaaatat aaataaaaaa gattacactt
      361 ttttcggtct aaaaataaaa tagaaaccta tcaatataaa cggtatacta agccattgac
      421 cggttgttaa ggcattaaaa aggggataag tttcaaaacc tccttgactt tcttttaaat
      481 attctacccc aaaacgaacg gaccaaagta ataccataaa tgttccaaaa ataagtcctt
      541 cctttttacg ggcatcagtt ttccaataca tgaaaaataa gattagaaaa acaaaaatat
      601 aaaagaaagc ctcatataat tgagaaggat gtttagcagg aacagcctcc aataaatttg
      661 aaaattgtgg gttttcagca atggctttgt aggcttcttt cggattagca atttgtgtat
      721 tttctaccgc ttctctttta gaataaaaat cctgaacaaa tttaattcca aaagaagaag
      781 ttgtttcatg ccccactatc tcagagttga aaaagtttcc taatctaaca aaaactgctc
      841 cactggcaac cggaataaca actctgtcta aaacccaaag tagggattta tgcatgattt
      901 ttttactgaa ataaaccatg aaaagaatga ttgcaatggc tgcaccatga ctggctaatc
      961 ctgaaaatcc ggtaaattca aaacttggtt ttgtgcgtat aggtagaaaa atgcttaatg
     1021 gatcttctct gaataattcg ggctgataaa aaataacatg ccctaatcga gcaccaatta
     1081 atgtagcaat aacggtatat ataaatagac tgtctaattt ttctaaggat tcattttctc
     1141 tgacgaaaat atatttcata atataccaac ctaggccaaa agcaatcacg aacattaaac
     1201 tgtaaaaacg aactgtaaac gaaccgattt caaaaccttc cgaaggattc caaacaaaac
     1261 ttaaaaattg ggttaccata tagacataat atttaatgta aatgtaaacg attaaattac
     1321 aaattgcaat ttgaatacgc taataaattc ttaaaagtag gagttaaggt acagggtcat
     1381 aaccactttt accccacgga tgacattttg aaatgcgttt tacagcaagc caaattcctt
     1441 ttattactcc atgtttaaca attgcttcgt gagaataact ggaacaagta ggttcaaaac
     1501 gacaagttgc cggagttaag ggtgaaatga ctacttgata aaaacgaatc aaaaaaataa
     1561 aagggaaacg aattattttt tttaacataa tagatttatt tcaattcgca acctaaacaa
     1621 cttaattaat tcacgctaaa acttgttcct tctttaccat ctttcaactg aataccaatg
     1681 gctaacaatt gatctcgaat ttgatcagat aaagcaaagt ttttattggc acgagcttcc
     1741 attcgcattt gaatcattaa attcacaact ccttcaagct tgtcaatgtt atcattacag
     1801 cttttttcat cagaaatgcc taatacatca taaacaaagg catgaagagt agaagttaat
     1861 ttttgaatat cagcggcagt taacgattct tttccatcgt ttaccaaatt gacaaagcga
     1921 atgccttcaa ataattgtgc aattaatata ggtgtattaa aatcgtcatt catggcatca
     1981 taacaacttt ttttccaatc gaaaatgtcc aaagttgaac tagaacttgc agataaattt
     2041 ttaagcaatt ccattccttc cattaatcga ttgaatcctt tttcagaacc caaaatagcc
     2101 tcattagaaa aatctaaaat acttctgtaa tgtgcctgaa gcataaaaaa tcgagccaca
     2161 tttggtgaaa aagctttgct taaaatttca ttttcgcctg taaatatttc attgggtaaa
     2221 atataatttc cggttgattt ggccattttt ttaccattta aaatcaacat attagcatgc
     2281 atccaaaaat taaccggact atgtcctgta caagcttcat tttgagcaat ttcacactca
     2341 tgatgcggga attttaaatc cattccgcca ccgtgtatat caaatgtttc acccaaatat
     2401 tttgtactca tagcagtaca ttctaaatgc caacccggaa aaccaatgcc ccacggagaa
     2461 ggccaacgca tgatatgttg cggttctgct ttcttccaca aggcaaaatc ctgcgggttt
     2521 tttttatcgg aaacaccatc taaatcacgg gtgttggcaa tcatatcttc aatatttcta
     2581 ccgcttaatt tgccgtaatg attggtttca ttgaatttga ctacatcaaa ataaacagaa
     2641 ccatttgcct cgtaggcaaa tccattttcg attatttttt taataatttc ctgttgttca
     2701 atgatatgac ccgtggctgt tggttctata cttggaggta aaaaattcaa cgtatgaaga
     2761 atgttatgaa aatctacggt ataacgttgc acaatttcca tgggttccaa ttgctcaatt
     2821 cttgcttttt tggcaattct gtcttcacct tcttccgcat cattttctaa atgcccggca
     2881 tcagtgatat ttctaacata ccgtactttg taacccaaat gaagtaaata acgaaaaata
     2941 acgtcaaaac tcataaatgt tcgaacattt cccaaatgta cattgctgta aacagttggc
     3001 ccacaaacat acatgcctac atttccttgg tggatagggg taaatacttc tttttcacct
     3061 gaaagtgaat tgtatatttt taaggtctga ttttcgtata atttcatttc tattctttct
     3121 gctttctaaa acttagtttc taacttaata taatctaaaa actctcttct aactttgtcg
     3181 tctttaaact ttccgccaaa ttcagaagta actgtgctac tttcaatgtc gcgaatacct
     3241 cttgaattga cacacaagtg tttggcatca atcacacaag ctacatcttc agtgttgagt
     3301 gctttttgca actcttggac aatctgcatc gttaaacgtt cttgaacttg aggtctttta
     3361 gcaaaataat caac
//
LOCUS       DAOTCW010000201         3350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_23015_length_3350_cov_28.253111, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000201 DAOTCW010000000
VERSION     DAOTCW010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23015_length_3350_cov_28.253111"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="P5335_11275"
     CDS             <1..444
                     /locus_tag="P5335_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HRZ75506.1"
                     /translation="GSFYFIAELIVDLELEYDSPTTDHCGSCTACIDACPTEAIVAPY
                     VVDGSKCISYFTIELKENIPTEMKDKFDDWIFGCDVCQDVCPWNRFSKSHNEPLFQAN
                     PELLSLSKKDWEEITEDVFKKVFKNSAVKRTKLEGLKRNVAFSKK"
     gene            complement(448..1233)
                     /locus_tag="P5335_11280"
     CDS             complement(448..1233)
                     /locus_tag="P5335_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75507.1"
                     /translation="MTNFSSLQLASSSTISILLFIFIIAFYILGHFFIKRAIRKDPEH
                     INIELGSINGALLALLGLILAFSFSMASSRFDTRRQLVIEEANNIGTAILRTDIYPDS
                     IKKMLKSNMKEYVEERIAFYQSGMNMQKTVEHYEKADFIGKKIWAITADYAKKDNITT
                     RTSELIPALNAMIDITTTRRAAGESTIPDSIMYFLFALCVCSAFLLGYDNKKTIDWII
                     LIGFTTMLTLTVFTIIDLDRPRSGLINMDGPNQKMVELLQMFE"
     gene            complement(1383..1997)
                     /locus_tag="P5335_11285"
     CDS             complement(1383..1997)
                     /locus_tag="P5335_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRZ75508.1"
                     /translation="MKNYFIILVLFLSFSVKAQEAGSLSLNLYGGYNFSDKVSFDMGY
                     VDVKEGFQWGVGLEYFVQKYSSVELKYLRIDTKMEAFVGNTQINAGDDKGAVNNILVD
                     FTQYFDAGSTKLLPYIGGGLGMGIVETPKNGNETTFAWDIKLGAKIKTSSAVSVNLHA
                     YLQSMSSAVGNDIFYTWYGPVYVTDYATLYQFGLGAVISYNFKN"
     gene            2261..>3350
                     /locus_tag="P5335_11290"
     CDS             2261..>3350
                     /locus_tag="P5335_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HRZ75509.1"
                     /translation="MHKDSKRREALLYHAKPSPGKIQVVPTKKYATQRDLSLAYSPGV
                     AEPCLEIAKDVNNVYKYTAKGNLVAVITNGTAVLGLGDIGPEASKPVMEGKALLFKIF
                     ADIDVFDIEVGTKDIDAFIETVKNIAPTFGGINLEDIKAPESFEIERRLVEELNIPVM
                     HDDQHGTAIISSAALLNALELADKKAENVKVVVSGAGSAALACANLYVLLGVKLENII
                     MFDSHGVLYHGRTDLSDLQIKYANGNEGTTLKEALIDADVFLGLSAGNILFPEMLLGM
                     AKDPIVFAMANPIPEIDYTLAINTRKDIIMATGRSDHPNQVNNVLGFPYIFRGALDVR
                     ATKINEEMKMAAVRALAELAKMPVPEQVN"
BASE COUNT         1172 a          521 c          558 g         1099 t
ORIGIN      
        1 ggttcttttt attttattgc tgaactaatt gtagatttag aattagagta tgattctcca
       61 accaccgacc attgtggttc gtgcacagcg tgtatcgatg cttgcccaac agaagccatc
      121 gttgccccat atgtggtaga tggcagcaaa tgcatctcct atttcactat tgagttaaaa
      181 gaaaatatcc caacagaaat gaaagacaaa ttcgatgact ggatttttgg ttgcgatgtc
      241 tgccaagatg tttgcccttg gaatcgtttt tcaaaatcgc ataacgaacc gctttttcaa
      301 gcgaatccag aattactttc cttgagcaaa aaagattggg aagaaatcac cgaagatgtt
      361 tttaaaaaag tatttaaaaa ttcagcggtt aagcgaacaa agctggaagg gttaaagagg
      421 aatgtggcgt tttcaaaaaa ataaaactca ctcaaacatt tgcagtaatt caaccatttt
      481 ctgattcggg ccgtccatat ttatcaaacc acttcgaggc cggtctaggt caattatagt
      541 gaatacagtt aaagtaagca ttgttgtaaa cccaattaaa ataatccaat caatggtttt
      601 tttgttgtca tatcctaaca gaaaagctga gcaaacacaa agagcaaaca aaaaatacat
      661 aattgaatcc gggatagttg attcacctgc cgctcttcgg gtagttgtaa tgtcaatcat
      721 ggcatttaga gccggaatta attcggaagt tcgagttgta atgttgtctt tcttagcata
      781 atcagcggtt atcgcccata tttttttccc aatgaaatca gctttttcat aatgttcaac
      841 tgttttttgc atattcattc ctgattgata aaaggcaata cgttcttcaa catattcttt
      901 catatttgat ttcaacattt tttttatgct gtcaggataa atatcagttc gtaaaatagc
      961 tgttccaatg ttgtttgctt cttcaattac taattgtctt ctggtgtcaa atcttgagct
     1021 tgccatacta aatgaaaaag ctaatattaa accaagcaat gccaataaag caccattaat
     1081 cgatcccaat tcaatattaa tatgttcagg atcttttcga atagcccttt taataaaaaa
     1141 gtgacccagt atataaaaag caataataaa aataaaaagt agtattgaaa ttgtagaaga
     1201 agaagctaac tgaagggaag aaaaatttgt cataaatata tagttatcag aataattttt
     1261 tttaaaaagt attataaatt tataattatt tctctgatta aaatagtact attgaattat
     1321 ttttgtaaac aaaaagcctt ttcatttaga atgaaaaggc tttaatttat aatttaatta
     1381 tattagtttt tgaaattata actaattaca gctcctaaac caaattgata taatgttgca
     1441 taatctgtaa cataaactgg accataccat gtgtaaaata tatcatttcc aacagcagat
     1501 gacattgatt gtagataggc atgtagatta acagaaacag cagaagatgt ttttatttta
     1561 gctcctaact taatatccca agcaaaagta gtttcattcc cgttttttgg agtttcaaca
     1621 atacccatac caagaccacc tccaatataa ggtagtagtt ttgttgatcc ggcatcaaaa
     1681 tattgagtaa aatcaactaa aatgttgtta acagctcctt tatcatcacc agcgttaatt
     1741 tgagtattac caacaaaagc ctccatttta gtatcaattc taaggtattt taattcaaca
     1801 gaactgtatt tttgaacaaa atattctaat ccaacacccc attgaaaacc ttctttgaca
     1861 tcaacataac ccatatcaaa acttactttg tcggaaaaat tataaccgcc atacaaattt
     1921 aacgacaatg atcctgcttc ttgggctttt acactaaatg ataaaaataa aactaatatt
     1981 ataaaataat ttttcataaa tttaaatttt aagtgattaa ataatttgta caaatttatg
     2041 ataaatttaa gaaattacat tgaaaatgaa atgttttttt atttgacata aaaaatacgg
     2101 atttgttttg attaataagt atataaaaaa atatattaat ttaaaatgtc agttttaagt
     2161 tgtgattttt ttaaattaaa taaaaaagaa tctatttaat atttccttat ttacttaact
     2221 cttttacctt tgtaggtcat agtagagaat accttaaatc atgcacaaag acagtaaacg
     2281 aagagaagcg ttattatacc acgcaaaacc aagtcccgga aaaattcaag ttgttcccac
     2341 caaaaaatat gcaactcaac gtgatttgtc attagcttat tcaccgggag ttgcagagcc
     2401 ttgtttggaa attgcaaaag atgtaaataa tgtttacaaa tacacggcca aaggaaattt
     2461 agtcgctgta attacgaatg gaacagcggt tttaggactg ggcgacattg gtccggaagc
     2521 ttcaaaacca gtcatggaag gaaaggcatt attgtttaaa atctttgcag atattgatgt
     2581 atttgatatt gaagttggta caaaagatat tgatgctttt attgaaactg taaaaaatat
     2641 tgctcctact tttggaggaa ttaatttgga agacataaag gctccggaat cctttgaaat
     2701 tgaacgtagg ctagtggaag agttgaatat accggtgatg catgacgacc aacatggaac
     2761 agccattatt tcctcagcag ctctgctaaa tgctttagaa ttagcggata aaaaagctga
     2821 aaatgttaaa gtagttgttt ctggtgccgg ttctgcagca ttagcatgtg ctaatttata
     2881 tgttttatta ggtgtcaaat tagaaaatat tatcatgttt gatagtcatg gtgttttata
     2941 tcatggaaga actgatttat ctgatttgca aattaaatat gcgaacggta atgaaggaac
     3001 tacattaaaa gaagcactaa ttgatgcaga tgtgttttta ggattatctg ccggtaatat
     3061 tttatttcct gagatgttgc ttggcatggc taaagaccct atagtgtttg cgatggctaa
     3121 tccaattcct gaaattgatt atactttagc tatcaatacc cgaaaagaca ttatcatggc
     3181 taccggtcgt tcagaccatc ctaatcaagt gaataatgtg cttggttttc catatatttt
     3241 tagaggagct ttggatgttc gggcaactaa aataaatgaa gaaatgaaaa tggccgctgt
     3301 tcgggcgtta gcagaattgg caaaaatgcc ggtgccggaa caagtaaata
//
LOCUS       DAOTCW010000202         3282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_23735_length_3282_cov_37.928726, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000202 DAOTCW010000000
VERSION     DAOTCW010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23735_length_3282_cov_37.928726"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1124
                     /locus_tag="P5335_11295"
     CDS             180..1124
                     /locus_tag="P5335_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRZ75510.1"
                     /translation="MTVWILFIAAIVLFLALDLGVFNKTPHIIKSREAGIWTAIWVTL
                     SFVFSGLIYWLYQNNYVNNPENYTPTIASMKYITGYLIELSLSVDNIFIIAVIFSSFK
                     IPQKYQHRVLFWGILGAIFFRGLMIFFGVILINKFSWMTYVFGLFLLFTAAKMLFKKE
                     EEQFNPKKSFVYRNLRKVMPVTMHMDGEHFFVKRRHITAATPLFVALVVIELMDVLFA
                     LDSVPAILAITPDPFLVFSSNIFAILGLRSMYFFLAHMLDKFKHLEYSLIAILSFVGI
                     KMLLVHNFKFPEWASLGFIAVALLIGILVSLYGSKKEE"
     gene            1359..2720
                     /locus_tag="P5335_11300"
     CDS             1359..2720
                     /locus_tag="P5335_11300"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRZ75511.1"
                     /translation="MKRNPRSNNVILNLGFEKLNEMQETAQETILTSTNTLLLSPTGS
                     GKTVAFLLPLVSLFDIESRKVQCLILVPSRELALQIEQVWKKMATGYKVTVCYGGHSI
                     ETEINNLSQPPAIVIGTPGRIADHLERGSFETTAVQTLILDEFDKSLQLGFHDEMAFI
                     ISKLPHVNKRVLVSATASIAIPGYTGAVSPTVLDFIPEEEGESGLSLKLVVSKEKDKM
                     STLFQLICSLHSEAALIFCNHREAAERISMLLNQRKIETTYYHGGMDQDERERALIQF
                     RNGSVNYLVTTDLAARGLDIPEMKHVIHYHLPGKAEEFTHRNGRTARMLQRGTAYIIY
                     HESEKKADYLDYDMPVFALEQGLKLPKSPLFKTVYISGGKKNKLNKMDIVGFFSQVGQ
                     LEKDDIGLLEVKDFVSFAAVKAAKVNDLLLRIKDQKMKGKKFKIEVARNVVKKDDEAK
                     RTR"
     gene            complement(2731..3138)
                     /locus_tag="P5335_11305"
     CDS             complement(2731..3138)
                     /locus_tag="P5335_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75512.1"
                     /translation="MNPIIKNILAVIAGLLVGSIVNMALITISGKVIPPPTDADITTM
                     EGLKASMHLFEPKHFLFPFLAHALGTLVGAFIAAKLAANNHIKIGLLIGVFFLVGGIA
                     NIVMLPSPPWFTVVDLVFAYLPMAYIGATLSRK"
BASE COUNT          897 a          525 c          771 g         1089 t
ORIGIN      
        1 caacagattt atttttttat ttgaaactgc aactatactt ttgactcacc atagcataag
       61 taggttaagt tcatggtaga tttggggcaa aaagggtgga agcaattcca ccttttttat
      121 ttgacagcat ggtgtaattg cttctacgtt tactactttt gtggtcttat ctttttatta
      181 tgactgtttg gattcttttt attgctgcaa tcgttttatt tttagcgtta gatttaggtg
      241 tttttaataa aacaccacac ataatcaagt ctcgtgaagc gggaatttgg actgcaattt
      301 gggttacact ttcctttgtt ttttccggat tgatttattg gttgtatcaa aacaattatg
      361 taaacaatcc tgaaaattat actcctacca ttgcttcgat gaagtatatc acagggtatt
      421 taattgaact ttctttgagt gttgataata tattcattat tgcagttatt ttctcatcgt
      481 ttaaaattcc gcagaaatac caacatcggg ttttgttttg gggaatatta ggagctatat
      541 ttttccgagg attgatgatt ttctttggtg ttatattaat aaacaaattt agttggatga
      601 cgtatgtttt cggcttgttt ttgttgttta cagcagccaa gatgttgttt aagaaagaag
      661 aagaacagtt taacccaaag aaatcgtttg tgtatagaaa ccttcggaag gtgatgcctg
      721 ttaccatgca catggatggc gaacactttt ttgttaaacg aagacatatc acggcggcga
      781 ctccgttgtt tgtggcgttg gttgtgattg aattgatgga tgttttgttc gcgttagaca
      841 gtgttccggc tatcttggca attacaccgg atcctttttt agtgttcagt tctaatatat
      901 ttgcgattct agggttgcgt tctatgtatt tcttcttggc tcatatgtta gataagttta
      961 aacatttaga atatagttta attgcaattc tatcctttgt gggtattaaa atgttgctcg
     1021 tgcacaactt taaatttccg gaatgggcgt cgcttggatt tattgcggtg gcacttttaa
     1081 tcggaatact tgtgtcactt tatggttcaa aaaaggaaga gtaattcatt tttgtaagtt
     1141 tattttacca ttcaaggatt atttcgttca atcatctaaa acattttttt tattactatc
     1201 atgatgctac atttggctca ccatagcata agtaggttaa gttcatggta gatttggggc
     1261 aaaaagggtg gaagcaattc cacctttttt attttatgcc ggttacttca tgcaccggca
     1321 ttcgaaacca acggataaaa aaaacttacc tttgccgcat gaaaagaaat cctcgctcta
     1381 acaatgttat cctgaattta ggttttgaaa aactgaatga aatgcaggaa actgctcagg
     1441 aaaccatttt aacttcgact aatacccttt tactctcgcc aacaggttcc ggaaaaacag
     1501 tggctttttt attgccatta gtgagtttgt tcgatataga aagtcggaag gtgcaatgtt
     1561 taatcttagt gccgtcgagg gaattggcgt tgcaaattga acaggtttgg aaaaaaatgg
     1621 caacgggtta taaagttacc gtttgttatg gagggcattc gatagagacg gaaataaaca
     1681 acctcagtca gccgccggca attgttattg gaacaccggg acggattgct gatcatcttg
     1741 aacggggttc gtttgaaaca acggcagtgc aaaccttgat tttagatgag tttgacaagt
     1801 cgttgcaact cgggtttcat gatgaaatgg cttttattat cagtaaatta ccccatgtta
     1861 ataaacgcgt gctggtttcg gcgacggcta gtattgctat tcctgggtat acgggagcgg
     1921 tatcacctac tgtgcttgat tttattccgg aagaggaagg ggaatcaggg ctttcgctaa
     1981 agctggtggt gtcgaaagaa aaggataaaa tgagcacgtt atttcaattg atttgttcgc
     2041 tccactcgga agcggctctt atattttgta atcatcgtga agcggcggaa cgcattagta
     2101 tgctgttaaa tcaacgcaaa attgaaacta cttattacca cggtggaatg gatcaggatg
     2161 aacgggaacg ggcgttgatt cagtttcgaa acggcagtgt gaactatctt gtgacgactg
     2221 atttagcggc acgcggttta gatattcctg aaatgaagca tgttattcat tatcatttgc
     2281 cgggcaaagc ggaagagttt acgcaccgca acggacgaac tgcccgtatg ttgcaacgcg
     2341 gaactgctta tattatttat cacgagagtg aaaagaaagc ggactacttg gattatgata
     2401 tgcctgtttt tgctttagaa caaggcttga agttgcctaa atcaccgttg tttaaaacgg
     2461 tttacattag tggtggcaag aaaaataaac ttaacaaaat ggacatcgtg gggttctttt
     2521 cacaagtggg gcaactggaa aaagacgaca tcgggttgct cgaagtaaaa gacttcgtgt
     2581 cgtttgcggc ggttaaagca gcaaaggtga atgacttgtt gcttcggata aaagaccaaa
     2641 agatgaaagg caagaagttt aaaattgagg ttgcccgcaa cgtggtgaag aaagacgacg
     2701 aggcgaagcg aacccgttaa tcaaactact ttattttcgg cttagtgttg ctcctatgta
     2761 ggccattgga aggtaggcaa acacgaggtc aacgacggtg aaccaagggg gtgacggtaa
     2821 catgacgatg tttgctattc caccaacgag gaagaatact cctattagta atcctatttt
     2881 tatgtggttg tttgcggcaa gttttgcggc aatgaatgct cctacgagtg ttccgagggc
     2941 gtgtgcgagg aagggaaaca ggaaatgctt gggttcaaaa aggtgcatgg aggctttgag
     3001 tccttccatg gtggtgatgt cggcatcggt ggggggaggg ataacttttc cgctgatggt
     3061 gatgagtgcc atgtttacga tgctgccgac tagtagccct gcgattacgg ctagtatgtt
     3121 tttgattatt ggattcattt tgtttgttat tggttagttg tgataaagat ataaaatttt
     3181 tgttttttag cctacgttgg ttaaaaatgg tgtttgtttg tgcttttagg gttggtgttt
     3241 atgattttat ggttggtctt ttaaaaaaga tggtcgctct tt
//
LOCUS       DAOTCW010000203         3213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_24457_length_3213_cov_49.423053, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000203 DAOTCW010000000
VERSION     DAOTCW010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24457_length_3213_cov_49.423053"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..>1651
                     /locus_tag="P5335_11310"
     CDS             165..>1651
                     /locus_tag="P5335_11310"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRZ75513.1"
                     /translation="MGLVTAKEVAKAINVDKYGFIGTFIGWIIMKVLKISTLNDIYNR
                     NKHLSDVPFLNAILDEFEIKFDIPEEDLKRLPKEGPYITVSNHPLGGIDGILLLKLML
                     EREPNFKIIANFLLHRIEPMKPYIMPVNPFENHKDAKSSVLGIKETLRHLSDGKPLGM
                     FPAGEVSTYKDGILVVDKPWEEGAIKVIRKAQVPVVPIYFHAKNSRLFYFLSSINETF
                     RTAKLPSELLTQKNRVIKVRIGKPISVAEQNEYETLESYSEFLRKKTYMLANAFEKGN
                     KLLAAPSLKFPKNPKEIAKPANHDKILEEMAMLKESDCRLLQSKNYHVYMITADKIPN
                     ILHEIGRLREITFRAIGEGTNESLDLDQYDKYYHHMFLWDDNAQMIAGAYRMGLGSEI
                     YAKYGIDGFYLHELFRFEPELYDMMSKSIEMGRAFIIEEYQQKPMPLFLLWKGIVHTT
                     LRYPEHKFLIGGVSISNQFSDFSKSLMIEFMKSHYYDPYVAQYIHP"
     assembly_gap    1652..1751
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2244..3188)
                     /locus_tag="P5335_11315"
                     /pseudo
     CDS             complement(2244..3188)
                     /locus_tag="P5335_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
BASE COUNT         1005 a          546 c          503 g         1059 t
ORIGIN      
        1 agtaaagttt ttcaaaaata gataaaaacc ttgagtatca aaacagactg gtggaattat
       61 aataaatgtt taacaagata cttctaatta gctgtttttt aaattttact ttctctataa
      121 ttactatatt tgttacttcc ataaaaataa acaacaaaac aagaatgggt ttagtaaccg
      181 ctaaagaagt tgcaaaagca ataaatgttg acaaatacgg tttcattgga actttcattg
      241 gctggattat tatgaaagtg cttaaaatat ctacacttaa tgatatttat aaccggaaca
      301 aacatttatc cgatgtgcct tttttaaatg ctattttaga tgaatttgaa ataaaatttg
      361 acattcctga ggaagattta aaacgtcttc cgaaagaagg tccgtatatc accgtttcca
      421 atcatccatt aggaggaatt gacggtattt tgttattaaa attaatgttg gaacgcgaac
      481 ctaattttaa aatcattgcg aattttctat tgcaccgcat tgagcccatg aaaccttata
      541 taatgccggt caatccattt gaaaatcaca aagatgctaa atcaagtgtt ttgggaatca
      601 aagaaacgtt gcgtcatttg agcgatggaa aaccgttggg aatgtttccg gccggtgaag
      661 tctccaccta taaagacggt atattagtgg ttgacaaacc ctgggaagaa ggagctatca
      721 aagtcattcg aaaagctcaa gttccggttg ttccaatcta ttttcatgca aaaaacagcc
      781 gcttatttta tttcttatcg agtattaatg aaacttttcg aacggcaaaa ttaccctcag
      841 aattgctgac acaaaaaaac cgtgtcatta aagttagaat tggaaaacct atttctgttg
      901 ctgaacaaaa tgaatatgaa acgctggaaa gctattcaga atttcttcgc aaaaaaacgt
      961 atatgctggc caatgctttt gaaaaaggaa ataaattgtt ggcagctcca agtttaaaat
     1021 ttccaaaaaa tccaaaagaa atcgcaaaac cggccaatca tgacaagatt ttggaggaaa
     1081 tggcaatgct caaagaaagt gattgccgtt tattacaaag taaaaactac catgtttata
     1141 tgattacggc cgataaaatc ccaaatattt tacatgaaat agggagatta cgtgaaatta
     1201 cgtttagggc tattggcgaa ggcacgaatg aatcattgga tttagaccaa tatgataaat
     1261 actatcacca catgtttttg tgggatgata atgctcaaat gattgccgga gcttaccgca
     1321 tggggttagg ttcagaaatt tatgcaaaat acggaattga tggtttttat ttacatgaac
     1381 tttttcgttt cgaaccggaa ttatatgaca tgatgagtaa gtctattgaa atgggcaggg
     1441 catttatcat agaagaatac cagcaaaaac caatgccttt gttcttgctt tggaaaggta
     1501 ttgttcacac cacgcttcgt tatccggaac ataaattttt aattggcgga gtgagtatca
     1561 gtaatcaatt ttctgatttt tcaaaatcgt tgatgattga atttatgaaa tcacattatt
     1621 atgaccctta tgtagctcaa tatattcatc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nttggtgtat tccaatcttc ttttggtttt gatgtttcca tttggttagt
     1801 gatttgatta aaactatttt ataaaaatat aaaaattatt gaaatgcttt gtcatttggt
     1861 tcccaaagtt caattttatt tccttctggg tctaaaatcc aagcaaattt tccatagtca
     1921 taagtctcca tatcacctac aattattaca ccttcattct ttaattcatc taatagatgc
     1981 aataaatttt ctactctgaa attttgcata aactgttttt cggatggcaa aaaataagtg
     2041 gtatcttctg caaaagggga ccattgggtt gaacaatcat ttccctcttt atctttccac
     2101 caaaaggtag aaccatattg atctgttttt aatcccaggt gtttatgata ccaatccact
     2161 aattttttgg aatcttttgc tttaaagaag aatcctccta ttcctgtcac tttctttaat
     2221 tcttgcctct cttctttcgg aaaataaatt gatttgattt tgtctactat aacctgagct
     2281 aatttttcat gactttcaaa agtccaaccg gcaatatgag gcgtaagtaa aacattctct
     2341 gctttcaata aatagtcaaa ggcagctggt ttatcactct caaatagcgt ttcaaaggat
     2401 agtttttcat attccagcac atctaatccg gctcctaaaa tctttccggt ttgtaaagct
     2461 tctactaaat cagctgttac aacagatttt cctctggcag tattcaaaaa ccaaaacggt
     2521 tttgcaaagg catttataaa ttctcgatta accattttat tcgtctcgaa agtccaaggg
     2581 gtgtgtaatg aaagtatatc agctttttgt tgtaattctt tcaaagaaac ttgttttgca
     2641 ttttgattgc caatgtttgg aagaatatcg taacaaatca cctcaacatc aaatccttgc
     2701 aattttttag caaacgattt tcccatgttg ccatatccaa taatgccaac tgtttttcct
     2761 tctaactcat acccacgatg tccttctcga atccaatgtc cgtcacgaac tgattgattc
     2821 gcctgattta atttgttcat caaggaaatt aacatgccta acgcatgttc acccacggca
     2881 tttctattgc cttccggtgc agcgattaat tgaatccctt tttctttggc ataatcacaa
     2941 tctatacttt ctaaacccgc tcctacccga gcgataaatt gaagatttgt cgctttatct
     3001 aaaaaagttt tatctatttt aaagcgacta cgaatcacaa ttccttggta gtgttggatt
     3061 ctattttcta tttcttcttt cgatgattga taatcttcgt gattactaaa accggcttgt
     3121 tctaactgat tccacaacaa aggatggttt gaatcaatat gaaggatatt tacagaagca
     3181 gttttcaatt tggtttgaat tagtaactat taa
//
LOCUS       DAOTCW010000204         3179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_24844_length_3179_cov_31.429898, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000204 DAOTCW010000000
VERSION     DAOTCW010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24844_length_3179_cov_31.429898"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2465
                     /locus_tag="P5335_11320"
     CDS             <1..2465
                     /locus_tag="P5335_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRZ75514.1"
                     /translation="ATQLEKIDMLDFADLVAINKFDKRGSLDALRDVKKQYQRNHNLW
                     DADLDLLPVFGTIASQFNDPGMNTLYKKVMDKIVEKTSADLKSTFEITREMSEKIFVI
                     PPHRTRYLSEIAENNRKYDETVLTQVEVAQKLYGIYKTIESVSHVHQLGLTKFGLDEE
                     RLLQAQSDSNKEFLSLLTKEFDRVKMNLDPFNWEIILTWDEKVNKYKNPVYSFKVRDK
                     VINIDTHTESLSHSQIPKVALPKYQAWGDILKWNLQENVPGEFPFASGLYPFKREGED
                     PSRMFAGEGGPERTNKRFHYVSAGLPAKRLSTAFDSVTLYGNDPDLRPDIYGKIGNAG
                     VSICCLDDAKKLYSGFDLSHPMTSVSMTINGPAPMLLGFFMNAAIDQNCEKYIIENNL
                     QVEVEEKINEIFKKKGVEKPKYNGELPQGNNGLGLMLLGVTGDQVLPADVYNDIKIKT
                     MAQVRGTVQADILKEDQAQNTCIFSTEFALRLMGDVQEYFIQKNVRNFYSVSISGYHI
                     AEAGANPITQLAFTLANGFTYVEYYLSRGMNINDFGPNLSFFFSNGIDPEYAVIGRVA
                     RKIWSKALKNKYGANERAQMLKYHIQTSGRSLHAQEIDFNDIRTTLQALYAIYDNCNS
                     LHTNAYDEAITTPTEESVRRAMAIQLIINKELGLAKNENPIQGSFIIEELTDLVEDAV
                     LQEFDRITERGGVLGAMETMYQRSKIQEESLYYETLKHNGDFPIIGVNTFLSSKGSPT
                     VVPREVIRATEEEKQFQINTLETLHQLHEQKVSELLNSLQEAAIKNQNLFERLMEVTK
                     YCSLGQITNALFEVGGQYRRNM"
     gene            2558..3052
                     /locus_tag="P5335_11325"
     CDS             2558..3052
                     /locus_tag="P5335_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75515.1"
                     /translation="MKKIFFLIIAISSLIACDSSDDSSLDFDSDIQVDGIEFTPTSAT
                     VSNATANMPGEGAMNFALTKGNVGTSDYESINFTVNFPLTSSSAPNGVYDFGIGGIGE
                     VLFAQGSYVKGSKVYSLAGYTVQVTVLSNTKYRLDFQNIQAVNPFDLEEMVVISGYYE
                     GEME"
BASE COUNT         1048 a          501 c          633 g          997 t
ORIGIN      
        1 ctgccacaca attggaaaaa attgatatgc ttgattttgc cgatttagtg gctatcaata
       61 aatttgataa aagaggttcg ttggatgcgt taagagatgt gaaaaaacaa taccaacgta
      121 accacaatct atgggatgcc gatttggatt tgcttcctgt ttttggaacc attgcttctc
      181 aatttaatga tccgggaatg aatacgcttt acaaaaaagt gatggataaa attgtggaga
      241 aaacaagtgc cgatttaaaa tcgacatttg agattacgcg agaaatgagc gaaaagattt
      301 tcgtgattcc gccacatcgc acaagatatt tgtctgaaat tgcagagaat aatcgcaaat
      361 acgacgagac tgttttaaca caagtggaag tagctcagaa attgtacgga atatacaaaa
      421 ccattgaatc ggtaagtcat gtacatcaat taggtctgac caaatttgga ttggatgaag
      481 aaagactttt acaggcccag tctgacagca ataaagaatt tttaagttta ttaaccaaag
      541 aatttgatcg tgttaaaatg aacttggacc catttaattg ggaaatcatt ttaacatggg
      601 atgaaaaagt aaacaaatac aaaaatccgg tttacagttt taaagttcgt gataaagtga
      661 tcaatataga cacacatacc gaatcacttt cgcattctca aatcccaaaa gtagcattac
      721 ccaaatacca agcatggggc gatatcttaa aatggaattt acaggaaaac gttcccggag
      781 agtttccctt tgcttcagga ttgtatcctt tcaaaagaga aggtgaagat ccatccagaa
      841 tgtttgccgg tgaaggcggt ccggaacgaa caaacaaacg ttttcattat gtaagtgctg
      901 gattacccgc caagcgttta tcaaccgctt ttgactcggt tactttgtat gggaacgacc
      961 ctgatttgcg tccggatatt tatggtaaaa taggaaatgc cggcgtttcc atttgttgtt
     1021 tagatgatgc aaaaaaatta tattctggtt tcgatttgag tcacccgatg acttctgtga
     1081 gtatgaccat taatggtccg gctcccatgt tgttaggctt tttcatgaat gcggcaatcg
     1141 accaaaattg tgaaaaatac atcattgaaa ataacttaca agtagaagtt gaagaaaaaa
     1201 tcaatgaaat cttcaagaaa aagggtgttg aaaaaccgaa atacaatgga gaacttcctc
     1261 aagggaataa cggattagga ttgatgttgt tgggagttac cggagatcaa gtattaccgg
     1321 ctgatgttta taatgatata aaaatcaaaa caatggctca ggttcgcgga accgtacagg
     1381 ccgatatttt aaaagaagat caagcacaga atacgtgtat tttttcaaca gaatttgctc
     1441 ttcgattaat gggagatgta caagaatatt tcatccagaa aaatgtgcgt aacttttatt
     1501 cggtttccat atcgggttat cacattgcag aagccggagc caatccaatc acacaattag
     1561 cttttacctt ggcaaatggt tttacgtatg tagaatatta tttgagtcgt ggaatgaata
     1621 tcaatgattt cggtccgaat ttatccttct ttttctccaa cggaatcgat ccggagtatg
     1681 ctgtgattgg tcgtgtggca agaaagattt ggtcaaaagc cttaaaaaat aaatacggag
     1741 ccaacgaaag agctcaaatg ttgaaatacc atattcaaac atccggtcgt tcgttgcatg
     1801 ctcaggaaat tgactttaat gacattcgta caacgcttca agctttatat gcaatttatg
     1861 ataattgtaa ttcgttacat accaatgcct atgacgaagc gattaccacg ccaacggaag
     1921 aatcggtgcg tcgtgcgatg gcaattcaat tgattattaa taaagaatta ggtttagcta
     1981 aaaacgaaaa cccaattcaa ggttccttta tcattgaaga attaactgat ttggtagaag
     2041 acgctgtact gcaagaattt gatcgaatta ctgaacgtgg tggtgtttta ggagcgatgg
     2101 aaactatgta tcaacgttct aaaattcaag aagaatcgtt gtattatgaa acgttgaaac
     2161 ataatggtga ttttccaatt attggcgtaa atacattttt gagttcaaaa ggttcgccga
     2221 cagttgttcc aagagaagtg atccgtgcaa cagaagaaga gaaacaattt caaataaata
     2281 cgttggaaac tttgcatcaa ttacacgaac aaaaggtttc agaattattg aattctttac
     2341 aagaagcagc tatcaaaaat caaaatcttt ttgaacgatt gatggaagta acaaaatatt
     2401 gttcgttagg gcaaattacc aatgcgttgt ttgaagtagg aggacagtat cgaagaaata
     2461 tgtagtatac aattttataa aaattctaaa acctttcatt tctgaaaggt tttaactttt
     2521 taatttcaat cagagtagta tttttacatt aaaaatcatg aaaaaaatct tttttttaat
     2581 tattgcaatt tcatctttga ttgcttgtga ttcttcagac gattcgtctc tagatttcga
     2641 ttcagatatt caagtggatg gtatagaatt tactccaaca agtgcaacag tttccaatgc
     2701 aacagccaat atgcctggtg agggtgctat gaattttgct ttgacaaaag ggaacgttgg
     2761 gacttccgat tatgaatcta ttaattttac tgtaaatttt ccacttacat caagtagtgc
     2821 acccaatgga gtttatgatt ttggaatagg ggggataggg gaagttttat ttgctcaagg
     2881 ctcgtatgtg aaaggttcaa aagtgtatag tttagcaggt tataccgttc aggttacggt
     2941 tttgtcaaat acaaaatacc gattggattt tcaaaatatt caagcagtca atccttttga
     3001 tttggaagaa atggttgtta tttcggggta ttacgaaggt gaaatggaat gatacattag
     3061 tcacgaaggc ttatttcctt cattactttt ttttataata gctttcaatt tttcttttct
     3121 agtcatttct ttttctttct ttttggcttg ctccttgtgc agtttgtcgt tgtgaattt
//
LOCUS       DAOTCW010000205         3137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_25375_length_3137_cov_46.086308, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000205 DAOTCW010000000
VERSION     DAOTCW010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25375_length_3137_cov_46.086308"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="P5335_11330"
     CDS             <1..1263
                     /locus_tag="P5335_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRZ75516.1"
                     /translation="AVRALAELAKMPVPEQVNIAYGETKLNFGREYIIPKPFDPRLIT
                     IVAPAVAKAAMDSGVAQYPITDWEKYEEELAERLGSDNKMVRLLANRAKTNPKRVVFA
                     EADHLDVLKAAQIVMEEGIGNPILLGNKEIILELKKEIGFDVDVPILDPKTSECDEKR
                     LHYANTFWESRKRRGISLLDAQKWMRERNYFAAMMVNEGEADALVTGFSRSYPSVVKP
                     IMQLIDKAPGVSKIATTNMMMTNRGPMFFSDTAINIDPSAHDLAKIALMTAKTVRLFG
                     MEPIIAMISFSNFGSSNTEGASKVREAVAYLHKYYPDLIVDGEIQTDFALNPEMLKNK
                     FPFSKLAGKKVNTLIFPNLEAANINYKMLKELYKVDSIGPIMLGLDKPVHIFQLGASV
                     EEMVNMAAVAVVDAQEKEKRYKSIKVTL"
     gene            1345..1926
                     /gene="ruvA"
                     /locus_tag="P5335_11335"
     CDS             1345..1926
                     /gene="ruvA"
                     /locus_tag="P5335_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRZ75517.1"
                     /translation="MIAHIQGRLVEKTPTDVVIDCNGVGYHVNISLHTYSLIPNTDSI
                     KLFTFLQIKEDAHTLFGFVEKSEREIFKLLLSVSGIGASIARTMLSSMEPKQILQAIA
                     SADVVTIQSIKGIGSKTAQRVILDLKEKVLKVYDLDEVSMPSNNTNKDEALSALEVLG
                     FNKKLAEKVVEKIARENPDATIESIIKQALKNL"
     gene            2011..>3137
                     /gene="sprA"
                     /locus_tag="P5335_11340"
     CDS             2011..>3137
                     /gene="sprA"
                     /locus_tag="P5335_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRZ75518.1"
                     /translation="MYSQDDETPKDTVGFNKGKVELSNPPSIVSAYTYDPVTDRYIYN
                     STVGEFNINYPVILTPKEYEELVMRESMKTYFKQKLDAMDGRKEGADEAKKDLLPRFY
                     VNSGFFETIFGSNTIDVQPRGSVEIDLGVRYTKQDNPTFSPRNRTTTTFDFEQRINMS
                     LSGKVGTRLNVNANYDTQSTFAFQNLIKLEYAPEEDAILRKLEVGNVSMPLNNTLIRG
                     AQSLFGVKAELQFGKTTVTGVYSEQKSQTRSVTALGDGTLQDFDLFALDYDSDRHFFL
                     SQYFRDKYDASLKNYPLINSRVQITRIEVWITNRQNRVNTSPEGNNLRNIIALQDLGE
                     AQLNGLDDTEIVGIDPIPAGFFNVLTDTPANNANNDFDPGL"
BASE COUNT         1086 a          461 c          609 g          981 t
ORIGIN      
        1 gctgttcggg cgttagcaga attggcaaaa atgccggtgc cggaacaagt aaatattgcc
       61 tatggtgaaa ccaaattaaa ttttggtcga gaatatataa ttccaaagcc cttcgatcca
      121 agattaatta ctatagttgc tcctgcagtt gcaaaagcag caatggattc aggagtggct
      181 caatatccaa tcactgattg ggaaaaatat gaagaagaat tagccgagcg tttaggttct
      241 gataacaaaa tggttcgttt gttggcaaat cgagctaaaa caaatccaaa acgtgtcgtt
      301 tttgcggaag ctgaccattt agatgtatta aaggcagccc aaattgtcat ggaagaaggt
      361 attggtaatc caattttatt agggaataag gaaattattc tagaacttaa aaaagaaata
      421 ggatttgatg ttgatgtgcc tattttagat ccaaaaacta gtgaatgtga cgagaaaaga
      481 cttcattatg ccaatacatt ctgggaaagt agaaaacgaa gaggaatttc tttgttagat
      541 gctcaaaaat ggatgcgtga acgcaattat tttgccgcta tgatggttaa tgaaggtgaa
      601 gcagatgctt tagttactgg attttcgaga agttatcctt ctgtagtcaa gccaatcatg
      661 caattgatag ataaagcacc cggagtttct aaaattgcca cgactaatat gatgatgacc
      721 aacagaggac caatgttttt ttcagatacg gcaatcaata ttgatccttc tgctcatgat
      781 ttagcaaaaa ttgctttaat gacagcaaaa actgttcgat tgtttgggat ggaaccaatt
      841 atagcaatga tttcattttc taattttggt tcatcaaata ccgaaggagc atcaaaagta
      901 agagaggctg tggcttattt gcataaatat tatccggatt taattgtaga tggtgaaatt
      961 caaaccgatt ttgctttaaa tcctgaaatg ttaaaaaaca aatttccttt ttctaaattg
     1021 gcagggaaaa aagttaatac gttgattttt ccaaatttgg aagcagcaaa tattaattat
     1081 aaaatgttga aagaattata taaagtagat tctattggtc caataatgct agggttagat
     1141 aaaccggtgc atatttttca attgggagca agtgtagaag aaatggtaaa tatggctgct
     1201 gttgccgtgg tagatgcaca agaaaaagag aaacgttata aatcgataaa agtcactctt
     1261 taactaatta tttaagttat taatcgtttt attggttgct aattaaagat ttttgctaca
     1321 tttgaaaatt aacatagaat aataatgata gctcatattc aaggacgttt agttgaaaaa
     1381 acgcctaccg atgtagtgat agactgcaac ggagtagggt atcatgtaaa tatttcattg
     1441 cacacttact ctttaattcc taatactgat agtattaagt tgtttacttt ccttcaaatt
     1501 aaggaagatg ctcatacgtt gtttggtttt gtagaaaagt cggaaagaga aattttcaaa
     1561 ttattacttt ccgtatccgg aattggagca agtattgcaa gaaccatgtt gtcttcaatg
     1621 gaacctaaac aaattcttca ggcaattgcc tctgcggatg tagtaacaat acaatcaata
     1681 aaaggaattg gatcaaaaac agcccaacgt gtcatattgg atttaaagga aaaagtgtta
     1741 aaggtgtatg atttagacga agtttctatg ccttcaaaca atacaaataa ggatgaagcg
     1801 ttatctgctt tggaggtatt gggctttaac aaaaaattag cagaaaaggt agtggagaag
     1861 attgctagag agaacccgga tgctacaata gaatcaataa ttaaacaagc tttaaaaaat
     1921 ttataatact tgaaaacgaa gtatcattat aattttatat caggtcttaa aattagtttt
     1981 atatggctaa tttttctttc cggtttttta gtgtattcac aagatgatga aacacctaaa
     2041 gacacagttg gtttcaataa aggtaaagtt gagttgtcta atccacccag cattgtctcg
     2101 gcatatactt atgatccggt aaccgatcgt tatatttata atagtactgt tggagagttt
     2161 aatatcaatt acccggtaat tcttacgcca aaagagtatg aagaattagt aatgcgggaa
     2221 tccatgaaaa cgtatttcaa gcaaaaattg gatgccatgg atggcagaaa agaaggtgcg
     2281 gatgaagcta aaaaagattt attgcctcgt ttttatgtta attccggttt ttttgaaact
     2341 atttttggaa gcaataccat tgatgttcaa cccagaggtt ctgttgaaat tgatttagga
     2401 gttcgctata caaaacaaga taacccaact ttttctccca gaaacagaac gactacaaca
     2461 tttgattttg aacaacgtat caatatgagt ttatccggta aagtgggaac tcgattgaat
     2521 gtgaatgcta attatgacac tcagtctacg tttgcttttc aaaatttaat caaattagaa
     2581 tatgctcccg aagaagatgc catcttacga aaattagaag taggtaatgt cagtatgcct
     2641 ttgaataata cgttaattcg aggtgctcaa agtttatttg gggtgaaagc tgaattacaa
     2701 tttggaaaaa caaccgttac cggagtttat tctgaacaaa aatcgcaaac tcgttctgtg
     2761 acagccttag gtgatggaac gcttcaagat tttgatttat ttgccttaga ttatgattca
     2821 gaccgacact ttttcttgtc gcaatatttt agagataaat atgatgcttc cttaaaaaat
     2881 tatcctttaa tcaatagtag agtacaaatt acccgaattg aagtttggat taccaaccga
     2941 caaaatcgcg taaatacttc gcctgaaggc aacaatttgc gaaatattat tgctttacaa
     3001 gatttaggag aggcacaatt aaatgggttg gatgatacag aaatagttgg aattgaccct
     3061 attccggcag gattttttaa tgtattaacc gatacaccgg ctaataatgc caataatgat
     3121 tttgatcccg gattaat
//
LOCUS       DAOTCW010000206         3120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_25576_length_3120_cov_37.626101, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000206 DAOTCW010000000
VERSION     DAOTCW010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25576_length_3120_cov_37.626101"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..827
                     /locus_tag="P5335_11345"
     CDS             36..827
                     /locus_tag="P5335_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRZ75519.1"
                     /translation="MKFTSKIYCFFIIAFLSIQTSIAQFTIPEIPVKQTSLYDYGDVL
                     QPEEERALEEKLIRYSDSTSTQIVVITIADLKGENIGLLAPQWGQAWGIGGSKEQDNG
                     VVILLARDDRKIHISPGYGVEDRLTAGITGELVRNVILPEFKAGSYYRGLDKGADAIF
                     DVLKGKYKGERKGNKGSGLPIILVLFFVMILIIIISKGSKNNRNGGTGKFSGPSLTDI
                     IILSSLGRGGFGGGSSSGGGFGGGGGFGGGFGGGGFSGGGAGGSW"
     gene            complement(887..>3120)
                     /locus_tag="P5335_11350"
     CDS             complement(887..>3120)
                     /locus_tag="P5335_11350"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRZ75520.1"
                     /translation="KDGKIATQNLPAEIIEKIQVVDTKTKEEEISGESASSEEKTINL
                     TIQEDKNKGFFGKAMAGYGTEERYESSLLLNWFKGERKLSVLASANNINSVGFSMNEI
                     FDNMGGGRNSSVYYSDDGSFGINGMTFGGSNGITTSNLVGLNYVDKWFKKVDMSSNYF
                     FTNAETDNVNRTSSTNLLPTGTTYTEADANSNRIGNSHNVSFDFEMKLDSTATIYMSP
                     KIAKSFNKDKYKSKQETTSASDELLNESLSDTYTETDNIAFENNLYFYKSFKKKNRGM
                     TISVDNEHKKTDLFSKNNSATLFYQSADPNDIRNQNQFAENKFNKMGTEIRFGEPLTD
                     SLKLSATTEILYYSSNESNQTYDFNDVSGEYDAFNAFQSNTINSSVLKINPTIGLNLQ
                     KKNIRARFNVGPEFVTFKNESDYLGITTPLNENYVFPKITGNLSYTISKSKSIYSNYD
                     YKVELPLANQILPVLNLSNPLNTYVGNADLKPTKQHSFYLNYNNYNWQERNGIFFYGG
                     MNLYKDQIVSSTVYDADFKATTTYQNVDSGASSYFGATWSKSFKKEKRNLRLSIGLDS
                     GYNFNQGLTNAELYEAKSWRAEPKVSLNWSIEELITISPSYRYTYNTTDYTNYQLDNA
                     NTFVHNFKLELTNYWPKNFVFGNDFGYTYNSNIADGFKNDFYLWNTSLGYNFYKDRLL
                     AKVKVYDVLNQNVGVRRTITPTAITDVENTVLQQYIMFSLTYKLDKFGGKKEGEGGIF
                     IID"
BASE COUNT          961 a          542 c          542 g         1075 t
ORIGIN      
        1 cgaacgaact ttctaatgaa atatccaaag gataaatgaa atttacttca aaaatttatt
       61 gcttttttat tattgcattt ctgtctattc agacatcgat agcccagttt actattccgg
      121 aaattcctgt taaacaaacc agtctatatg actatggtga tgttttacaa cctgaggaag
      181 aaagagcttt agaagaaaaa ctaattcgct attccgacag tacctctact caaattgtag
      241 tgattaccat tgccgattta aaaggtgaaa acatcggatt gttagctcct cagtggggtc
      301 aagcttgggg aataggtgga agtaaagaac aagataatgg tgtggtgatt ttattagcca
      361 gagatgaccg aaaaattcat atttctcccg gatatggtgt ggaagacaga ttgaccgcag
      421 gaattaccgg tgaattagta cgaaatgtta ttcttcccga gtttaaagct ggaagttatt
      481 accgtggatt agataaagga gccgatgcta tttttgatgt tttaaaaggc aaatataaag
      541 gggaacgaaa aggaaataaa ggcagtggtt taccaatcat tttagtcctt ttctttgtaa
      601 tgatattgat tattattatc tcaaaaggtt ctaaaaataa tcgtaacggt ggaacaggaa
      661 aattttccgg accgagctta accgatatta tcatcttgag tagcttaggc cgaggtggtt
      721 ttggcggtgg ttcctcatcg ggtggaggtt ttggtggtgg cggaggtttt ggtggcggtt
      781 ttggaggtgg tggctttagt ggtggcggtg ccggtggaag ttggtaaatt aaccattaaa
      841 attttataaa aatgcgttta gattaaaccc taaacgcatt tttcttttaa tctataataa
      901 aaattcctcc ttcaccttct ttctttcctc caaatttatc taatttatat gttagtgaaa
      961 acatgatgta ttgttgcaaa acagtgtttt ctacatctgt aattgcggta ggcgttatgg
     1021 ttcgccgtac gccaacattt tgattcaata catcataaac ttttactttg gccaacaaac
     1081 ggtctttata aaagttatag cctaaactgg tattccataa ataaaaatca tttttaaaac
     1141 catcggcgat gtttgaatta taggtatacc cgaaatcatt cccgaaaaca aaatttttag
     1201 gccaatagtt tgtaagttcc aatttgaaat tatgtacaaa tgtattggcg ttgtccagtt
     1261 gataatttgt ataatcagta gtattatacg tgtaacgata agaaggtgaa atggttataa
     1321 gttcttcaat actccaattc aaacttactt taggttctgc tcgccatgat tttgcttcat
     1381 ataattcagc attggttaac ccttggttga aattataacc cgaatctaaa cctatactta
     1441 atcgtaggtt tcttttctct tttttaaagg atttactcca agtggctcca aaataactac
     1501 tcgctccgga atccacattt tgataggttg tagtagcttt aaaatcggca tcataaaccg
     1561 tagaagaaac tatttgatct ttgtacaaat tcattccacc ataaaaaaag attccattac
     1621 gctcctgcca attgtaattg ttatagttaa gataaaaaga atgctgttta gtgggtttta
     1681 aatcggcatt tcccacataa gtattcaacg gatttgaaag gttcaacacg ggaagaattt
     1741 gattagccaa gggcaattct actttataat catagttgga ataaatcgac ttgctcttac
     1801 tgatggtata acttaaattt cctgtgattt taggaaaaac atagttttca tttaagggtg
     1861 tcgtgatgcc taaataatcg gattcattct taaaagtcac aaactctggc cccacattaa
     1921 aacgggctct gatattcttt ttttgaagat taagcccaat agtggggttt atttttaaca
     1981 cacttgaatt gattgtatta gattgaaaag cattaaaagc atcatattct ccggaaacat
     2041 cattaaaatc atacgtctga ttagattcgt tcgaactgta atataaaatt tcagtcgtgg
     2101 cactcaactt taacgaatcg gttaagggtt ctccaaaacg gatttctgtc cccattttat
     2161 taaacttatt ttcggcaaat tgattttgat tcctaatatc attcggatca gcggattgat
     2221 aaaaaagggt ggccgaattg ttcttcgaaa acaaatccgt ttttttatgt tcattatcca
     2281 ctgaaatggt cattcctctg tttttctttt taaacgattt gtaaaaataa aggttgtttt
     2341 caaaagcaat attatctgtt tcggtatagg tatctgaaag actttcattt aaaagttcat
     2401 cggaagcaga ggtagtttcc tgcttgcttt tatacttatc tttattaaaa ctcttggcta
     2461 ttttcggact catataaata gttgcggtag aatccaattt catttcaaaa tcaaaactga
     2521 cattatgaga attaccaatt ctatttgaat tcgcatcagc ttctgtatag gttgttccgg
     2581 tgggcaataa attagtgctg gaagttcgat tcacattatc cgtttcagca ttggtaaaaa
     2641 aataattact gctcatgtca acttttttaa accatttgtc cacataattc aaccccacta
     2701 aattggacgt agtaatccca ttacttcccc caaaagtcat tccgttgatg ccaaaactcc
     2761 catcatcact ataataaaca gacgaatttc ttccacctcc catgttatca aaaatttcat
     2821 tcatagaaaa acctacagaa ttgatgttat ttgccgaagc caaaacactc agctttcgct
     2881 cacccttaaa ccaatttaaa agtaaactcg attcataacg ttcctctgtg ccataaccgg
     2941 ccatggcctt cccaaaaaag cctttatttt tatcttcctg aatagttaaa ttgatagttt
     3001 tttcttctga agaagccgat tctcctgaaa tttcttcttc ctttgtttta gtatcaacca
     3061 cttgtatttt ttcaataatt tctgccggaa gattttgggt ggctattttt ccgtctttac
//
LOCUS       DAOTCW010000207         3111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_25675_length_3111_cov_47.963678, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000207 DAOTCW010000000
VERSION     DAOTCW010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25675_length_3111_cov_47.963678"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="P5335_11355"
     CDS             <1..843
                     /locus_tag="P5335_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRZ75521.1"
                     /translation="QVQKTNVRIVAATNVNMFDAIEKGKFREDLFYRLSTVDIHLPPL
                     RERKEDIHLLFRKFAADFAQKYKMPAIKLEDNAVDLLLKYRWSGNIRQLRNIAEQISV
                     LETNRNISYQTLYSYLPQEGSNLPALVKDKKSESDFSNEREILYKVLFDMKSDLNDLK
                     KLTLELMQNGSSKVQEANKNLIQKIYGQKEDTEIDFEEPRTDVISSQNLSQQVEFEED
                     DDDQNYLFAETVEEEETLRLDAKEIELIKKALDRNKGKRKAAADELGISERTLYRKIK
                     QFDL"
     gene            850..1356
                     /locus_tag="P5335_11360"
     CDS             850..1356
                     /locus_tag="P5335_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HRZ75522.1"
                     /translation="MKKSIQFSLLIIILFSINGCKYYNFTGTGKIDAKTFQVNYFQNN
                     AELIEPGIERDFTLALQDLIQNQTNLSLTNSGGDLIYEGEITGYRISPMTATADQKTA
                     QNRLTMTVNVRFTNTKKEEDNFEKPFTFFHDYPASSQLVGSTLQAAKDDIFERITQDI
                     FNASLAKW"
     gene            1436..2446
                     /locus_tag="P5335_11365"
     CDS             1436..2446
                     /locus_tag="P5335_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRZ75523.1"
                     /translation="MNLSEYTYLLNNPNAVNEKQAKALETVVDSFPFFQSARALYLKG
                     LYNQDSYKYNFALKQTAAHTTDRSVLFDFITSDEFTIIEKDIYEQKLAQLMDIEVVES
                     NIDYAIFTETNNVIEIQDVEESTLPTVEFEPIADIEFIKVEELETTVNNSEPEEKIIP
                     VEIVNEVISVDITNDKQIAEENTIAEEKLQLGKPISFTQNEQHSFQEWLQLASINPIE
                     RKTEVSKPLFSAKPIEKEDEKVGNVSDKQKKLEIIDKFIETNPKITPAREVVETPVKS
                     IEISDTTHLMTETLARVYLEQKKFSKAIQAYEILILKYPEKSIFFADRIKDIKILQQN
                     NQ"
     gene            2449..2796
                     /gene="secG"
                     /locus_tag="P5335_11370"
     CDS             2449..2796
                     /gene="secG"
                     /locus_tag="P5335_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRZ75524.1"
                     /translation="MGFSVFLVLITVVCFLLIVVIMVQNPKGGGLSSTFGSSQQIGGV
                     QKTTDFLDKSTWTLGIILIALIMLSSLAFNDGDGTVKIDKSSAPATTETAKPATTPAP
                     VESTPAETAPKPE"
     gene            2954..>3111
                     /locus_tag="P5335_11375"
     CDS             2954..>3111
                     /locus_tag="P5335_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRZ75525.1"
                     /translation="MSVNIKPLSDRVLVEPAAAETTTASGIIIPDTAKEKPQRGTVVA
                     VGNGKKDYT"
BASE COUNT         1206 a          491 c          505 g          909 t
ORIGIN      
        1 caagtacaaa aaacaaatgt tcgtatagta gctgctacaa acgtaaatat gttcgacgct
       61 atcgaaaaag gaaaattccg tgaagattta ttttaccgat taagcacggt tgacattcat
      121 ttgccccctt tacgggaacg aaaagaagat attcatttat tatttcgaaa atttgctgct
      181 gattttgctc agaaatacaa aatgcccgcc attaaattag aagacaatgc agttgattta
      241 ttgcttaaat accgatggag cggaaacatt cgtcagttaa gaaatattgc cgaacaaatc
      301 tctgtcttag aaaccaatag aaacatttca tatcaaacat tatattccta tttgccgcag
      361 gaaggctcta atttaccggc attagtaaag gataaaaaat ccgaaagtga ttttagtaat
      421 gaacgtgaaa ttttatacaa agtacttttc gacatgaaaa gtgatttaaa cgatttgaaa
      481 aaactcacgt tagaactgat gcaaaatggc agttctaaag tacaggaagc aaataaaaat
      541 ttaattcaaa aaatttacgg tcaaaaagaa gataccgaaa tcgactttga agaacctaga
      601 actgatgtta tttcgtcaca aaatctttca caacaagttg aatttgaaga agatgatgac
      661 gaccaaaact atttatttgc agaaacagtt gaagaagaag aaacattgcg attagatgca
      721 aaagaaattg aactcatcaa aaaagcctta gacagaaaca aagggaaacg taaagcggca
      781 gccgatgaat taggaatttc tgagcgaaca ttataccgaa aaattaaaca atttgattta
      841 taaaaaaata tgaaaaaatc cattcaattt agtttattga taattattct gttttccata
      901 aacggttgta aatattataa ttttaccggc acaggaaaaa ttgatgctaa aacatttcag
      961 gttaattatt ttcagaataa tgccgaatta attgaaccgg gaattgaacg ggatttcacc
     1021 ttagcattac aagatttaat tcaaaatcaa accaatttaa gtctaacaaa ttcaggcgga
     1081 gatttaattt atgaaggcga aattaccggt tatagaattt ctccgatgac agctactgct
     1141 gatcaaaaaa cagctcaaaa ccgtttaacc atgaccgtca acgtgcgatt taccaataca
     1201 aaaaaagaag aagataattt tgaaaaacct tttacttttt tccatgatta tccggcaagt
     1261 agtcaactgg ttggaagcac tttacaagct gccaaagatg atatttttga acgcattaca
     1321 caagatattt ttaatgcctc tttagcaaaa tggtagtttt tagttttaaa ttgccaggta
     1381 ttgttaattt tttctaactt tgaataggaa tctaaaagtt aaccgataac aaaatttgaa
     1441 tttatccgaa tacacatatc tgctcaataa cccgaatgcg gtaaatgaaa aacaagccaa
     1501 ggcattagaa acagtggttg acagttttcc tttctttcaa agtgctcgtg ccttatattt
     1561 aaaaggctta tacaaccaag acagttataa atacaacttt gcattaaagc aaactgctgc
     1621 tcatacaact gatcgaagtg ttttatttga cttcattaca tcagatgaat ttactatcat
     1681 tgaaaaagat atttatgagc aaaaactagc tcaactcatg gatattgaag ttgttgaaag
     1741 caatattgat tatgctattt tcactgaaac caataacgtc attgaaattc aggatgttga
     1801 agaatccact ttaccgactg tagaatttga accaattgca gatattgaat ttattaaagt
     1861 ggaagagcta gaaactactg taaataatag cgaacctgaa gaaaaaataa taccggtaga
     1921 aatagtaaat gaggtaattt ctgttgacat aacaaatgac aaacaaattg ctgaagagaa
     1981 tacgatagct gaagaaaaat tacaacttgg taaacccatt tcgtttactc aaaacgagca
     2041 acattctttt caggaatggt tacaattagc aagtattaat ccaattgaga gaaaaactga
     2101 ggtttcaaaa ccgttatttt cggcaaaacc aatagaaaaa gaagatgaaa aagtaggaaa
     2161 tgtttcagat aaacaaaaga aattagaaat tattgacaag tttattgaaa caaatcccaa
     2221 aataactccg gcacgagaag ttgttgaaac ccctgtgaaa tctattgaaa ttagtgatac
     2281 aacacactta atgactgaaa cattagctcg agtttattta gaacaaaaaa aattttcaaa
     2341 agcaattcaa gcttatgaaa tattaatttt gaaatatccg gaaaaaagta ttttctttgc
     2401 agaccgaatt aaagacatta aaattttaca acaaaataat caatagcaat gggattttca
     2461 gtttttttag tattaataac agtagtatgt tttttactta tcgtagtaat tatggttcaa
     2521 aaccctaaag gtggcggttt atcatctact tttggcagtt cacaacaaat aggtggcgtt
     2581 caaaaaacaa ccgatttttt agataaaagc acttggacat taggcattat cttgattgca
     2641 ttgattatgc tttctagttt agcttttaat gatggtgacg gaacagttaa aattgacaaa
     2701 agttctgcac cggctactac agaaacagca aaacctgcta ctactccggc acccgtagaa
     2761 tcaacaccgg ctgaaacagc accaaaacca gaataatatt atttatttat atagaaaaaa
     2821 tcccgataac tcgggatttt tttttgaata tctagtattt gaattcactg aaaaaatgtc
     2881 agttactagc aattggcata atttctgact aatagtaaag cattaaattt aattaaataa
     2941 aaaataaata tttatgtcag taaacattaa acccctttca gatcgcgttt tagtggaacc
     3001 ggcagcagcc gaaacaacca ctgcatcagg aattattatt ccggacactg ctaaagagaa
     3061 acctcaacga ggaacagttg tcgctgttgg aaacggcaaa aaagattaca c
//
LOCUS       DAOTCW010000208         3088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_25983_length_3088_cov_50.505440, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000208 DAOTCW010000000
VERSION     DAOTCW010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25983_length_3088_cov_50.505440"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="P5335_11380"
     CDS             <1..1938
                     /locus_tag="P5335_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRZ75526.1"
                     /translation="VLGMNRRGNRLALYNRADYGYETRSELMNFTLPIVISSQKYLVH
                     FDNPPIGYLDLDSKKENILEYETISGRKTYQVIVGDSWENLVSNYTSLTGKQPLPPRW
                     ALGNFSSRFGYHSEAEARRTIQKFQDEKIPVDAIILDLYWFGKEVQGTMGNLEIFRDS
                     FPTFEKMVADFNKMGIKTIPITEPFVLTTSKKWQEAVDKKVLATDSNGKPFTYDFYFG
                     NTGIIDIFNPEGKNWFWDIYKDLGNKGVGGVWGDLGEPEVFPAAARTFGGTADEVHNI
                     YGHNWAKLVFEGYQKDFPNQRPFILMRSGYSGSQRYGMIPWSGDVNRTWGGLSGQVEI
                     SLQMGMQGLAYMHSDLGGFAGANLDDELYVRWLQYGVFNPIFRPHAQSDVASEPIFRS
                     DWAKNLAKQAIVLRYQLLPYNYNLAFENSQTGKPFMRPLFFEEPENELLLNKSDGYLW
                     GKDFLIYPIMEAKQKEKEVYFPKNATWFDFYSQEKYEGGTSQKVSIVENHIPTFVRGG
                     SFIPMANLVQTTERYNANTMSFHYFLDKKTTTHSNQFYFDDGATVNAFEKETYEIIKL
                     HVQNESKKTQIEIEREAGKNYQTAFKICTFIIHNVEAKPKNVQFNGKKTKFTYDEKTT
                     TLTIGITYFGDQKEKIEILFR"
     gene            1935..>3088
                     /locus_tag="P5335_11385"
     CDS             1935..>3088
                     /locus_tag="P5335_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRZ75527.1"
                     /translation="MKKILILILALISLTNCSTKKQAMTTETPFVWEGANLYFLLIDR
                     FNNGNPENDKIIPRDKETGVLRNFMGGDIKGITQKIEEGYFTDLGINAIWMTPVVEQI
                     HGAVNEGTGNTYGFHGYWTKDWTAIDPSYGTRKDLAELVEKAHAKGIRIVLDAVINHT
                     GPVTELDPAYPESWVRVGPKCTYDTYQNYIDCTLVENLPDVKTESNENVELPPMLVEK
                     WKKEGRYEQEVAELDAFFKRTGYPRAPKYYIMKWLADYITDFGIDGYRVDTVKHTTED
                     VWADFSKVCKDAFAQFKKDNPKKVLDANEFFLVGEVYGYGISGKQLYNFGDKKVNYFE
                     NGFVSLINFDFKGDANKQYEETFSNYSTLLNSDLKGLSVMNYVTSHDDGWP"
BASE COUNT         1033 a          532 c          612 g          911 t
ORIGIN      
        1 gttttaggca tgaaccgtcg cggcaatcga ttagctcttt acaatcgtgc tgattatggt
       61 tacgaaaccc gttcggaatt gatgaatttc acccttccaa ttgtaatttc ttcccaaaaa
      121 tacttggttc attttgacaa tcctcccatt ggttatttgg atttagacag taaaaaagaa
      181 aatattttag aatacgaaac tatttccggc cgcaaaacct atcaggtgat tgtaggtgat
      241 tcgtgggaaa atttagtttc aaattatacg tcattaaccg gtaagcaacc gttaccacca
      301 cgatgggcgt taggtaattt ctcctctcgt tttggttatc attctgaagc cgaagccaga
      361 agaaccattc aaaaatttca agacgaaaaa atcccggttg atgccattat tttagattta
      421 tattggtttg ggaaagaagt gcaaggcacc atgggtaatt tggaaatttt cagagacagc
      481 tttccaactt ttgaaaaaat ggtggctgac ttcaataaaa tgggaatcaa aaccattccg
      541 attacggaac cctttgtttt gacaacttcc aaaaaatggc aggaagccgt tgataaaaaa
      601 gtcttggcaa cggattcaaa cggaaaacct tttacctacg atttttactt tggaaacacc
      661 ggaatcatcg acattttcaa ccccgaaggg aaaaattggt tttgggatat ttataaagat
      721 ttaggcaaca aaggcgttgg tggtgtttgg ggcgatttag gagaacccga agtttttccg
      781 gctgcggctc gaacttttgg aggaacagct gacgaagtac acaatattta cggtcataat
      841 tgggccaaat tagtttttga aggttatcag aaagatttcc ccaaccaaag accttttatc
      901 ttgatgcgtt ccggttattc gggttcacaa cgatatggga tgattccgtg gagtggcgat
      961 gtgaaccgaa cgtggggcgg attatccgga caagtcgaaa tttcattgca aatgggaatg
     1021 caaggcttag cttatatgca ctctgattta ggcggatttg ccggtgcaaa tttagatgat
     1081 gaattgtatg tgcgttggtt gcaatacggt gttttcaatc cgatattccg tccgcatgca
     1141 caatcagatg tggcttctga accaattttt agaagtgatt gggcaaaaaa tttagctaaa
     1201 caagcgattg tattgcgtta tcaattgctt ccttataatt acaatttagc ttttgaaaac
     1261 tcacaaaccg gaaaaccatt catgcgacct ttgttttttg aagaaccgga aaatgaatta
     1321 cttctcaaca aatccgatgg ttatttatgg ggaaaagatt ttttgattta tccgattatg
     1381 gaagcaaagc aaaaagagaa agaagtctat ttcccaaaaa atgctacttg gtttgatttt
     1441 tacagtcagg aaaaatacga aggcggtact tctcaaaaag taagcatagt agaaaatcat
     1501 atcccaacat ttgttcgcgg tggaagtttt atcccaatgg caaatttggt tcaaactact
     1561 gaacgctata atgccaacac catgagtttt cattatttct tagataaaaa aacaacaaca
     1621 catagtaacc aattttattt tgatgatggt gccactgtta acgcttttga aaaagaaacg
     1681 tatgaaatca tcaagttaca tgtacaaaac gaaagtaaaa aaacacaaat tgaaatcgag
     1741 cgagaagccg gtaaaaacta tcaaacagcc tttaaaattt gtacttttat cattcacaat
     1801 gtagaagcaa aacctaaaaa tgttcagttt aatggtaaaa aaacaaaatt tacctatgat
     1861 gaaaaaacaa caacactaac tattggcatc acctattttg gtgaccaaaa agaaaaaatt
     1921 gaaattctat tccgatgaaa aaaatactaa tactcatttt agcactcata agtttgacca
     1981 attgttctac caaaaaacaa gcgatgacta ctgaaactcc ttttgtttgg gaaggtgcca
     2041 atttatactt tctcttaatt gaccgtttta ataacggcaa cccagaaaat gacaagatta
     2101 ttccgcgtga caaagaaacg ggagtacttc gcaattttat gggaggtgat atcaaaggaa
     2161 tcacgcaaaa aattgaggaa ggttatttta ccgatttggg aattaatgcc atttggatga
     2221 cccctgtagt cgaacaaatt catggtgctg taaacgaagg caccggaaac acttatggtt
     2281 ttcatggcta ttggacaaaa gattggacag ccatcgaccc aagttatgga actcgtaaag
     2341 atttagccga attggttgaa aaagcacatg ccaaaggcat ccgaattgtc ttggatgctg
     2401 tgattaatca cacgggacct gtgaccgaat tagaccctgc atatcccgaa agttgggtac
     2461 gtgttggccc gaaatgcacc tatgatactt atcaaaatta cattgattgt acgttggttg
     2521 aaaatctacc ggatgtaaaa accgaaagta atgaaaacgt agaattacct ccaatgctag
     2581 ttgaaaaatg gaaaaaagaa ggtcgttatg aacaagaagt tgctgaatta gatgcctttt
     2641 tcaaacgaac cggttatccg agagctccga aatattatat catgaaatgg ttggcagatt
     2701 acatcactga tttcgggatt gatggttatc gtgtagatac cgttaaacat accaccgaag
     2761 atgtttgggc tgatttttca aaagtttgta aagatgcttt tgctcaattc aaaaaagata
     2821 atcctaaaaa agtattagat gcgaatgaat tctttttagt gggcgaagtt tacggttacg
     2881 gaatcagtgg caaacaactc tataattttg gcgataaaaa agtaaattat tttgaaaacg
     2941 gttttgtttc ccttattaat tttgatttta aaggcgatgc caataaacaa tatgaagaaa
     3001 ctttctccaa ctattcaacc cttttaaatt ctgatttgaa aggattgagt gtgatgaatt
     3061 atgtcacttc ccacgacgat ggttggcc
//
LOCUS       DAOTCW010000209         3061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_26338_length_3061_cov_44.151031, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000209 DAOTCW010000000
VERSION     DAOTCW010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26338_length_3061_cov_44.151031"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..930
                     /locus_tag="P5335_11390"
     CDS             145..930
                     /locus_tag="P5335_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRZ75528.1"
                     /translation="MISYSIYKNDSSSEWVTFVHGAGGSSSIWFKQIREFQKHFNVLL
                     LDLRGHGESKPSLKDGFQKKYTFKSIAHDVIEVIDHLKIEASHFVGISLGTIVIRQLA
                     EMHPSRVKSMIMGGAILKMNFRSQILMRLGNMFKYVLPYLVLYKFFAFVIMPKKNHKQ
                     SRLLFINEAKKLYQKEFIKWFKLTAEINPVLKWFRQVEINIPTLYVMGEEDYMFLPSV
                     KQVVEKHYRSSKLFVIENCGHVVNVEQPNQFNKAVISFLKSKV"
     gene            complement(1117..1821)
                     /locus_tag="P5335_11395"
     CDS             complement(1117..1821)
                     /locus_tag="P5335_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRZ75529.1"
                     /translation="MKKLPLLFTFFILLGCAEMQQIANQIPQTGSILSNADIANGLKE
                     ALNNGIEKQVSKLTKTDGFFKNETVKILLPDELKIVDTRLRSIGLSNLADEGLKVLNR
                     AAEDAVKEATPIFVDAVKQMTFSDAKNILMGNESAATSYLKNTTSTALYSKFNPVIKS
                     SFSKVGADKVWTNIISKYNSIPLVTKINPDLTDYTTNKAMDGVFKMIAVEEKQIRTNL
                     NARSSDILRKVFALQD"
     gene            2037..>3061
                     /locus_tag="P5335_11400"
     CDS             2037..>3061
                     /locus_tag="P5335_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRZ75530.1"
                     /translation="MEQATPYIPKNKVRIVTAASLFDGHDAAINIMRRIIQATGVEVI
                     HLGHDRSVEEVVNTAIQEDANAIAMTSYQGGHNEYFKYMHDLLKEKGAGHIKIFGGGG
                     GVILPEEIKDLHDYGITRIYSPDDGRAMGLQGMINDLVQKADFPVGDVLNGEAKHLED
                     KNPRSIARVISAAENFPDIAKETLDIIHKENETSKIPVLGITGTGGAGKSSLVDELVR
                     RFLIDFPKKTIGLISVDPSKRKTGGALLGDRIRMNAINNPRVYMRSLATRQSNLALSK
                     YVAEAIQVLKAAKYDIIILETSGIGQSDTEIIEHSDVSLYVMTPEFGAATQLEKIDML
                     DFADLVA"
BASE COUNT         1024 a          468 c          521 g         1048 t
ORIGIN      
        1 atttctttat tttctttaaa aacgcctttc attggggtga aattttttgc aaatatacag
       61 tttcaaaact gtattatttc aatgaatttt gttaagattg gaaatgtcaa ttaaaatgtt
      121 acttttgtct aaaagatata aaaattgata tcgtatagca tttataaaaa cgattcgagt
      181 tcggaatggg taacttttgt tcatggagca ggaggaagtt cgtctatttg gtttaaacag
      241 atacgtgaat ttcaaaaaca tttcaatgta ttactcttag atttacgtgg tcatggtgaa
      301 tctaaaccaa gtttaaaaga tggttttcaa aaaaaatata cctttaaatc aatcgctcat
      361 gatgtgattg aagtgattga ccatttgaaa attgaagctt ctcattttgt tggaatttcg
      421 ttaggaacta ttgtgattag acaattggca gaaatgcatc cttcaagggt gaaatcgatg
      481 attatgggtg gagcaatttt aaaaatgaat tttcgttctc aaattttgat gcgattggga
      541 aatatgttca aatatgtttt accttatttg gtattatata aattttttgc ttttgtgatt
      601 atgcctaaaa aaaatcacaa acaatcacgt ttacttttta tcaatgaagc caaaaaatta
      661 taccagaaag aatttatcaa atggtttaaa ttaacggctg aaataaatcc ggttttaaag
      721 tggtttcgtc aggtagaaat caatattcca accctatatg tcatggggga agaggattat
      781 atgtttttac cttctgttaa acaagtggtg gaaaaacatt accgttcgtc aaaattattt
      841 gtgatagaaa attgtggtca tgtagttaat gttgaacaac caaaccagtt taataaagca
      901 gttatctctt ttttaaaatc aaaagtataa aaaaagtcag tggtctcaaa cactgacttt
      961 aatctaaaac taactaacta acctaaccaa acaaactttt ttattgatgc aaatatcaga
     1021 agaattgtta gcttattgtg ttaagccttt gttatatata tgttattgat tattaaattt
     1081 ttaaggctga tttaatagat agtctttttt ctattttcaa tcttgtaatg caaaaacctt
     1141 tcttaaaata tctgaacttc tggcattcaa attagtacgt atttgttttt cttctacagc
     1201 tatcatttta aaaacaccat ccatcgcttt attggttgta taatctgtta aatcgggatt
     1261 gatttttgta accaatggaa tagaattgta cttggatata atattggtcc aaactttatc
     1321 agcacccact tttgaaaagg aacttttaat aaccggatta aattttgaat acaaagctgt
     1381 tgaagttgta ttttttaaat agctggttgc tgccgattca tttcccatca aaatattttt
     1441 tgcgtcagaa aaagtcatct gctttacggc atcaacaaaa atcggagtag cttctttcac
     1501 agcatcttct gcagcacgat ttaaaacttt taaaccctca tctgctaaat ttgataatcc
     1561 aatggagcgt aatcgagtgt caactatttt taattcatca ggtagcagaa tttttacggt
     1621 ttcattttta aaaaaaccat ctgtttttgt cagttttgat acttgtttct cgataccatt
     1681 atttaaagct tccttcaaac cattggcaat atcggcatta ctgaggattg accctgtttg
     1741 agggatttgg ttggctattt gttgcatttc tgcacaacct aaaagaataa aaaaagtaaa
     1801 aagcagaggt aattttttca tggtatatat aagttttgat tttaaaagta gacaaatatc
     1861 atgcctttaa atagcacaca gaacatctaa aattaagttc atcaattttt cttaaatcaa
     1921 taacatctct taatggttgt tctgaaacta tttttgtaat tcatttttct gatttttcat
     1981 ctacaaattt tattaattcc gtaaattgca ccctcaaaaa catcattttt ttagcaatgg
     2041 aacaagcaac accttatatt cctaaaaata aagtaagaat tgtaacagcc gcatcccttt
     2101 ttgacggaca cgatgcagcc atcaacatta tgcgtcgaat tattcaggca actggagttg
     2161 aggtgattca tttgggtcat gaccgaagcg tggaagaagt agtaaatact gccattcaag
     2221 aagatgccaa tgcaattgca atgacgtctt atcaaggcgg gcataatgaa tatttcaaat
     2281 atatgcacga tttgctcaaa gaaaaaggag ccggtcacat taaaatattt ggtggaggcg
     2341 gaggcgtaat tctaccggaa gaaatcaaag atttacacga ttacggaatc actagaattt
     2401 attctcctga tgatggtcgt gccatgggat tgcaaggaat gattaacgat ttggttcaaa
     2461 aagctgattt tcctgttgga gatgttttaa atggtgaagc caaacactta gaggataaaa
     2521 atccgcgttc tattgccaga gtaatttctg ctgcagagaa tttccctgat attgctaaag
     2581 aaaccttaga tattattcac aaagaaaatg aaacctccaa aattccagtt ctcggaatca
     2641 ccggaacagg aggagccgga aaatcatcat tagtagatga attggttcgt cgatttctaa
     2701 tcgattttcc taaaaaaaca atcggattaa tttctgtaga tccatccaaa agaaaaacag
     2761 gtggagcttt gttaggagac agaatacgaa tgaacgctat taacaatcca agagtttata
     2821 tgcgttcgtt ggcaactcgt caatccaatt tagccttatc taaatatgtt gccgaagcca
     2881 ttcaagtgtt gaaagcagca aaatacgata ttattattct ggaaacttcc ggaattggtc
     2941 aaagtgatac ggaaattatc gaacattccg atgtttcgtt atatgtaatg actcctgaat
     3001 ttggtgctgc cacacaattg gaaaaaattg atatgcttga ttttgccgat ttagtggcta
     3061 t
//
LOCUS       DAOTCW010000210         3011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_26944_length_3011_cov_45.685724, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000210 DAOTCW010000000
VERSION     DAOTCW010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26944_length_3011_cov_45.685724"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /gene="hemF"
                     /locus_tag="P5335_11405"
     CDS             <1..620
                     /gene="hemF"
                     /locus_tag="P5335_11405"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HRZ75531.1"
                     /translation="PMVPTVHANWRYFEMYDDNGKIINSWFGGGQDLTPYYLFEDDAI
                     HFHQTCKTACDKHNLEYYPKYKKQCDNYFWNAHRNEARGIGGLFFDYCKATETVSMDD
                     WFNFVTEVGNSFLEAYVPIVEKRVHLPYSQQHRTWQEIRRGRYVEFNLVHDKGTLFGL
                     KTNGRIESILMSLPPHVQWVYDHHPEKGSEEEKLITILANPKEWL"
     gene            611..1552
                     /locus_tag="P5335_11410"
     CDS             611..1552
                     /locus_tag="P5335_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HRZ75532.1"
                     /translation="MAIKTVIPLFFGGILSTFAQQDSQSKPHFKNYNEKVIFSVYYSD
                     ISNSFDVKLNNSTGADYFEFKPNKREQIGVNLSYKFIDVSYGFSPSFFEINKDNENSK
                     LFTLSTRLYQKQWMQSIIFINQTGFYISQGDIELYFEDLRTTKIGGTTSYVFNENFSF
                     RTIANQKEWQSISAGSFIPNFSFYYTNFDLNDGNDDSKSDIFLISLSPSYFYNWVIND
                     YFLLSGGLIAGGGIDIVDSDTSPIFEWGASLKVGYNSDSFFTFLNLNYIDFLQDSDAN
                     VRVNDEISMVKITAGYRFNPPKKVKEYYDKSEKKIGL"
     gene            1586..2575
                     /gene="hemB"
                     /locus_tag="P5335_11415"
     CDS             1586..2575
                     /gene="hemB"
                     /locus_tag="P5335_11415"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRZ75533.1"
                     /translation="MYPLRRNRRLRTNESIRSLVRETILSPNDFIVPLFVVEGKGIKE
                     EIPSMPNYFRLSLDLLEKEVKELAKLNLKSVLVFVKVPDHLKDNKGTEALNPNGLMQR
                     AVKVIKNAVPEMIVMTDVALDPYSEYGHDGVIENGVIVNDLTSAILAEMSCSHAEAGA
                     DIVAPSDMMDGRILEIREALEDEGYVNTGIMAYSAKYASAHYGPFRDALDSAPVDMID
                     IPKDKKTYQMDYHNRIEAIRETRMDIDEGADIVMVKPGISYLDIVREVKNAFDVPVAV
                     YQVSGEYAMIKAASERGWIEHDAIMMEQVTAIKRAGADMIASYFAKDVVKIIS"
     gene            2645..>3011
                     /locus_tag="P5335_11420"
     CDS             2645..>3011
                     /locus_tag="P5335_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C552"
                     /protein_id="HRZ75534.1"
                     /translation="MKKLFFLLVSMVFISCGEKKSEPFGKSTDENLTEPIAVTADASL
                     KLGEDLFNGVGLCKTCHLPDKKVIGPSIIDVATIYKEKNGNIVEFLKENAEPIVDPTQ
                     YEVMKTNFALTKSMSDEELK"
BASE COUNT         1048 a          428 c          594 g          941 t
ORIGIN      
        1 gtccaatggt gccaaccgtt catgccaatt ggcggtattt tgaaatgtat gatgacaatg
       61 gaaaaataat taactcatgg tttggtggcg gtcaggattt aacgccttat tatctttttg
      121 aagacgatgc gatacatttt catcaaacgt gcaaaacggc atgcgacaaa cacaatttgg
      181 agtattaccc gaaatataaa aagcaatgtg acaactattt ttggaatgcc catagaaatg
      241 aagcccgagg tattggcgga ttattttttg actattgcaa agcaaccgaa acagtttcca
      301 tggacgattg gtttaatttt gtaaccgaag taggcaatag ttttttagaa gcctatgttc
      361 caattgttga gaaaagagta catttgcctt atagtcaaca acatagaact tggcaagaaa
      421 tccgtcgagg tcgttatgta gaattcaatt tagtgcatga caagggcact ttatttggat
      481 tgaaaaccaa tggcagaatt gaaagtattt tgatgagttt accaccgcat gtacaatggg
      541 tgtatgacca tcatccggaa aaagggagtg aagaagaaaa attaattact attttagcaa
      601 atccgaaaga atggctataa aaacagtaat accgttgttt tttggaggaa tcttgagcac
      661 atttgcccaa caagattctc aatctaaacc acattttaag aattacaacg aaaaagtaat
      721 ttttagtgtt tattattctg atatttctaa tagttttgat gtaaaactca acaactcgac
      781 aggagctgat tattttgagt ttaaacctaa taaacgtgag caaattgggg taaatttaag
      841 ttataaattt attgatgttt catatggatt ttccccctct ttttttgaaa taaataaaga
      901 caatgaaaac tctaaattat ttactttgag tactcgatta taccaaaagc aatggatgca
      961 gtcgataatt tttattaatc aaacaggttt ttatatttct caaggtgaca tcgaactata
     1021 ctttgaagac ttgcgaacaa caaaaattgg aggaacaacg tcttatgttt ttaatgaaaa
     1081 tttttctttt agaactattg caaatcaaaa agagtggcaa tctataagtg ccggcagttt
     1141 tattcctaat ttttcatttt actatactaa ttttgactta aatgatggga atgatgatag
     1201 caaaagcgat atttttctaa tttcactatc tccatcttat ttttacaatt gggtgataaa
     1261 tgattatttt ttgctttcgg gaggtcttat tgcagggggt ggaatcgata tagtagatag
     1321 tgatacatct ccaatatttg aatggggagc aagtttaaaa gtagggtata actcagattc
     1381 attttttact ttcttaaatt taaattatat cgacttttta caagattcag acgccaatgt
     1441 tcgagtaaat gatgaaatta gcatggtaaa aattacagca ggttatcgtt ttaacccacc
     1501 caaaaaagta aaagaatatt acgataaatc agaaaaaaaa atcggattgt aaatacataa
     1561 atctacaatc attaaattaa aaaaaatgta cccacttaga agaaacagaa gattgcgaac
     1621 taacgagtct attcgttcat tggttcgcga aaccattcta tcaccaaacg atttcatcgt
     1681 gccgcttttt gtggtcgaag gaaaaggaat caaagaagaa atcccttcga tgcccaatta
     1741 tttcagattg agtctggatt tattggaaaa agaagtgaaa gaattggcca aattaaacct
     1801 taaatcggtt ttggtttttg taaaagtacc cgatcattta aaagataata aaggaaccga
     1861 agcattgaat ccaaacgggt tgatgcaacg agcggtaaaa gtgattaaaa atgctgttcc
     1921 ggaaatgatt gtaatgacgg atgtggctct agatccatat tctgaatatg gtcatgatgg
     1981 tgtgattgaa aacggtgtga ttgtaaatga tttaacttcg gctattttgg cagaaatgag
     2041 ttgttcgcat gctgaagcgg gtgccgatat cgtcgctccg agtgatatga tggatggacg
     2101 aattttagaa atcagagaag cattggaaga cgaaggttat gtaaacaccg gaatcatggc
     2161 ctatagtgct aaatatgcat cggcacatta tggtccgttt cgcgatgctt tggacagtgc
     2221 tcctgttgac atgattgata ttcccaaaga taaaaaaacc tatcaaatgg attatcataa
     2281 tcgtattgaa gccattcgtg aaaccagaat ggacattgac gaaggtgctg atattgtgat
     2341 ggtaaaaccg gggatttcgt atttggatat tgttcgtgaa gttaaaaatg catttgatgt
     2401 tccagtagca gtatatcaag tttcggggga atatgccatg ataaaagcag catccgaaag
     2461 aggttggatt gaacatgacg ccatcatgat ggaacaagta acggcgatta aaagagccgg
     2521 tgccgatatg attgcaagtt attttgcaaa agatgtggtg aaaataattt cttaaaatga
     2581 tatttatcag ttatttaaaa ttttttaaat cgtaaatttg agttgataaa aaaaatggaa
     2641 ttttatgaaa aaattatttt ttttactcgt ttctatggtt tttatttctt gtggagaaaa
     2701 aaaatcggaa cctttcggaa aatctactga tgagaattta acggaaccta tagcggttac
     2761 cgcagacgca agtcttaaat taggagaaga cttatttaat ggtgttggat tatgtaaaac
     2821 atgccattta cctgacaaaa aagtaattgg ccctagtatt attgatgtgg caactattta
     2881 taaagagaaa aacggaaaca tagtggaatt tttaaaagaa aatgccgaac caatagttga
     2941 ccctacgcaa tatgaagtga tgaaaactaa ttttgcttta acaaaaagta tgtccgatga
     3001 agagttaaaa g
//
LOCUS       DAOTCW010000211         2999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_27102_length_2999_cov_40.360394, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000211 DAOTCW010000000
VERSION     DAOTCW010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27102_length_2999_cov_40.360394"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="P5335_11425"
     CDS             <1..402
                     /locus_tag="P5335_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRZ75535.1"
                     /translation="TVKKSILKHFDIKQAVFYADFNWNLILKLISNKIKFQDIPKYPE
                     VRRDFALLLDQHITFDSLYAIASQTEKSMLKEISLFDVYEGNNLPEGKKSYALSFHIQ
                     DTTKTLTDEQIDKIMNKLKLNFEKELGAVLR"
     gene            complement(529..>1380)
                     /locus_tag="P5335_11430"
     CDS             complement(529..>1380)
                     /locus_tag="P5335_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRZ75536.1"
                     /translation="IADYGAFIEVAEGVEGLIHVSEMSWSTHLRSAQDFMKIGDEVEA
                     VVLTLDRDERKMSLGIKQMTQDPWTDITSKYPVGSKHKGIVRNFTNFGIFVELEEGID
                     GLIYISDLSWTKKIKHPSEFVNVGETLDVVVLELDVDGRKLSLGHKQTTANPWDKHED
                     AYAVGTVHNGTIAEIVDKGATVDFGDDVVAFVPTRHLEKEDGKKLKKGDAADFKVIEF
                     NKEFKRVVASHTAIFREEEEKNVKAATETVAANNNNNTNAPAQTLGENNQILADLKAK
                     MEKGEKK"
     assembly_gap    1381..1480
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1481..1809)
                     /locus_tag="P5335_11435"
     CDS             complement(<1481..1809)
                     /locus_tag="P5335_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRZ75537.1"
                     /translation="MSEILKEQEAFLANFNWHNFQEGIDAVDEKNLREFEELVAKTFI
                     STDSDEVVMGTVVRITDRDAIVDINAKSEGVISLNEFRYNPGLKVGDKVEVLIDVRED
                     KTGQLVLS"
     gene            complement(2009..2806)
                     /locus_tag="P5335_11440"
     CDS             complement(2009..2806)
                     /locus_tag="P5335_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRZ75538.1"
                     /translation="MFNFSNKNVLITGGASGIGKLMGELVLQKGAKLIIWDINQKNID
                     ETRSQFSKFGTVVGYNVDVADIEQVKITAKIVKEEHGSVDVLINNAGIIVGKYFHEHS
                     NDDITKTMNINANAPMHIALAFLSDMMKQNSGYICNIASSAGLISNPKMAVYAASKWS
                     VIGWSDSLRLEMNQLKKNIGVTTVTPYYISTGMFNGVRSIIPLLKPQKVAYKIIKGIE
                     HNRIFVSMPWSVHFVRFFQGLLPIWLFDWFVGKVMGVYNTMEHFKGR"
BASE COUNT          960 a          540 c          404 g          995 t
ORIGIN      
        1 actgtcaaaa aatcaatcct aaaacacttc gatattaaac aagctgtttt ttatgccgat
       61 ttcaattgga atttaatttt aaaacttatt tcaaataaaa ttaaatttca agacattcca
      121 aaatatcctg aagttcgcag agattttgct ttactattag atcaacatat cacatttgat
      181 tctttatatg ctattgccag tcaaaccgaa aaatctatgt taaaggaaat aagtttgttt
      241 gatgtgtatg aaggaaataa tttaccggaa ggaaaaaaat catatgcttt gagctttcat
      301 attcaagata ctacaaaaac cttgacagat gagcagattg ataaaattat gaataaactc
      361 aaactaaatt tcgaaaagga attgggtgcc gttttaagat aattgtttac tataaatcag
      421 aaaagccacg taataacgtg gttttttttg ttcaaattag ttttgacata acaaaaaaag
      481 tcccctcgtt tgcacgagag aacttttaga tttataaaaa tttgagaatt attttttctc
      541 tcctttttcc attttagctt taaggtctgc caaaatttgg ttattttcac caagtgtttg
      601 agcaggagcg tttgtgttat tattattatt tgcagcaact gtttcagtag cagctttcac
      661 atttttctcc tcttcttctc tgaaaatagc cgtgtgcgaa gcaacaactc ttttgaattc
      721 tttgttgaat tcaattactt tgaagtcagc agcatcacct tttttcaatt ttttaccatc
      781 ttctttttct aaatgacgag ttggtacaaa agcaaccaca tcatcaccaa aatcaactgt
      841 tgctcctttg tcaacaatct cagcgatagt accgttgtga acagttccta cagcataagc
      901 atcttcatgt ttatcccaag gattagccgt agtttgtttg tgacctaacg ataatttacg
      961 tccgtcaaca tctaattcta agacaacaac atctaacgtt tcacctacat tcacaaattc
     1021 agatggatgt ttgattttct tagtccaaga taaatcagag atataaatta aaccatcgat
     1081 accttcttct aattctacga aaataccaaa gtttgtaaag tttctaacga tacctttatg
     1141 tttagaacca actgggtatt tagaagttat atcagtccaa ggatcttgcg tcatttgttt
     1201 gatacctaaa gacatttttc tttcgtctct gtctaaagtc aatacaactg cttctacctc
     1261 atcaccaatt ttcataaaat cctgagccga acgtaaatgc gttgaccatg acatttcaga
     1321 aacgtggatt aaaccttcaa caccttcagc aacttcaatg aaagcaccgt aatcagcgat
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gataaaacca attgaccggt
     1501 tttgtcttca cgaacgtcga ttaaaacttc tactttgtca cccactttta aacctgggtt
     1561 gtaacggaat tcatttaaag aaataactcc ctctgatttc gcgttgatgt caacgatagc
     1621 atcacgatct gtaatacgaa ccactgttcc cattactact tcgtcactgt cagttgaaat
     1681 gaaggtttta gcaaccaact cttcaaattc tcttaggttt ttttcatcta ctgcatcaat
     1741 tccttcttgg aagttatgcc agttaaagtt tgctaaaaac gcttcttgtt cttttaaaat
     1801 ttcagacatg ctgattaaaa atttgtattc tgagttctct tgagtttctt gatgcgatag
     1861 aaaacagcag aagttgttta cataaatagt ttatacccaa tggaaactct tcttcgccaa
     1921 aaggtgtgca aaagtacgaa gcttttttgt aattacaaaa ctatttttca agcaattatg
     1981 ccaattgaaa aagattttcg tttgtgaatt aacgtccttt aaaatgttcc attgtattat
     2041 aaacgcccat gaccttgccc acaaaccaat caaacaacca aataggcaat aatccttgaa
     2101 aaaaacgaac aaaatgaaca ctccacggca tacttacaaa aatgcggttg tgttcaatac
     2161 cttttatgat tttataagca actttttggg gtttcaacaa aggaataatg gaacgaacac
     2221 cattaaacat tccagtactg atataatagg gagttacagt tgtaacacca atattttttt
     2281 tcagttggtt catttctaaa cgcaaactat ctgaccaacc aataacagac catttactgg
     2341 ctgcataaac ggccattttc gggtttgata ttaaacccgc agaagaagca atattgcaga
     2401 tgtatcctga attttgtttc atcatatcag aaagaaatgc taaagcaatg tgcatgggag
     2461 cattcgcatt gatattcatt gttttagtaa tatcatcatt agaatgttca tgaaaatatt
     2521 tcccaacaat tattccggca ttgttaatca ggacatctac agatccatgt tcttccttaa
     2581 caattttagc cgttattttg acttgctcaa tatcagcaac atccacatta tagccaacca
     2641 ctgttccaaa ctttgaaaat tgtgaccgtg tttcatcaat attcttttga ttaatgtccc
     2701 atataataag ttttgcccct ttttgcaaaa caagttctcc cattaatttc cctattccgg
     2761 aagctccacc ggtaatcaga acatttttat ttgaaaaatt aaacatgaaa agttttttaa
     2821 attaagtttc aaatttaaag attgttattc aaataaatag tttaaaaaaa ctcttttatt
     2881 cactaggatt aatattcttt acaagttgac tttcttcaaa tgtaaaccga taaaaattat
     2941 tacttttgaa aacagtcaat cctgtttcta aatttatgaa aacaaaattc ttagttata
//
LOCUS       DAOTCW010000212         2989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_27247_length_2989_cov_47.549080, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000212 DAOTCW010000000
VERSION     DAOTCW010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27247_length_2989_cov_47.549080"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..644)
                     /locus_tag="P5335_11445"
     CDS             complement(135..644)
                     /locus_tag="P5335_11445"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRZ75539.1"
                     /translation="MQPKFPSDSITTLTDIVLPGETNPLNNLFGGELLARMDRAASIT
                     ARRHSRRICVTASVNHVAFNKAIPLGSVVTVESKVSRAFNTSMEIFIDVWIEDRESGI
                     KSKANEAIYTFVAVDETGSPIAVPQVDPQTELEKQRFEAALRRKQLSLVLAGKMKPHD
                     ATELKALFV"
     gene            complement(757..>1269)
                     /locus_tag="P5335_11450"
     CDS             complement(757..>1269)
                     /locus_tag="P5335_11450"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRZ75540.1"
                     /translation="EVEKLEEKTPILFTPEKRTSSWMKYAAIFAFGIGMAGFFGNQWY
                     EKKIANETLLVEKAVQQKVENKIQQATFFIENPIPSVTLTVEEESNSNNQYHIVAGAY
                     KEISNADRALQSLLDLGYKAKKISPNKHGLYPILYGSYATIEEAKTALQTIKETHNKD
                     AWMLVLELEK"
     assembly_gap    1270..1369
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1370..1859)
                     /locus_tag="P5335_11455"
     CDS             complement(<1370..1859)
                     /locus_tag="P5335_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRZ75541.1"
                     /translation="MRIEQHISQLLYRYQCVTVPGFGAFLTENRSAQLVQDTNTFYPP
                     KKVLSFNFHLKNNDGLLANHISLCEKIDYDKAVALIEEEVSAWKFSIQKSEALTLKNI
                     GKLSLNSENSLVFEPIDNLNYATDSFGLTSFISPKIKREVYKKEVEKLEEKTPILFTP
                     EKR"
     gene            2076..>2989
                     /gene="dprA"
                     /locus_tag="P5335_11460"
     CDS             2076..>2989
                     /gene="dprA"
                     /locus_tag="P5335_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRZ75542.1"
                     /translation="MTTSELYFTLALLQVEGVGDIVAKKLINHCGSAEKVFDTKKNRL
                     LSIDGIGEILLKNLSDKSVFKLAQQEIDFVEQEKIAFHSFLDEKYPDRLKHCIDGPPI
                     IFSSGNIDLQAKRIISIVGTRKITSYGTEFCKKLIEDLAPLNPVIVSGFAYGVDIMAH
                     QVAMEHNLQTIGVLAHGLNQVYPKTHKKYISKMEQNGGFLTEFWSTSNPEKENFVKRN
                     RIVAGMSEATIVIESADRGGSLITATLANDYNRDVFAVPGRITDKYSQGCNNLIKTQR
                     AHLLSSAADLVYILNWELENKKTKTIQK"
BASE COUNT          972 a          465 c          476 g          976 t
ORIGIN      
        1 ttaaaatttt cagtactaca aaatgatacg ttcttaaatc ctcaaagttg gatttataat
       61 atgtaaaagt gaatttcaaa ttaaaaaagc caaacttttt ataaatcgtt tggctttttt
      121 attctaagtt aaaactatac aaacaaagct tttagttctg ttgcatcatg aggtttcatc
      181 ttgccggcta aaactaaact caattgtttt cgtcttaaag cagcttcaaa acgttgtttt
      241 tctaattctg tttgcgggtc tacttgagga acggctattg gagaaccggt ttcatcaact
      301 gctacaaaag tatagattgc ttcatttgct ttacttttaa tcccggattc tctgtcttca
      361 atccaaacat ctataaaaat ctccatagaa gtattaaaag ctcttgaaac ttttgattct
      421 acagtgacaa cactgcccaa tggaatagcc ttgttaaagg caacgtgatt aacggaagct
      481 gttacacaaa tacgtctgga atgtcttctg gcagtaatac tggcagctct atccatgcgg
      541 gctaataact cgcctccaaa aagattgttt aatggatttg tttcacccgg caaaacgatg
      601 tctgttaatg ttgtaataga atcagaagga aattttggtt gcatacttta tttttttaca
      661 aaggtagaaa aaccaaagta aatcattgag gaaagagtag gtataaaaaa aagcttcagt
      721 taagaagctt taaaatcaat attttgggca aagggtttat ttttctagct ctaaaaccaa
      781 catccacgcg tctttattat gcgtttcctt tatggtttgt aaagcagttt tagcttcttc
      841 tattgtggca tagcttccat acaaaatggg atataagcca tgtttatttg ggctaatttt
      901 cttggctttg taacctaaat ccaataaaga ttggagggct ctatcagcat tgctgatttc
      961 tttataggca ccggcaacaa tatgatattg attattagaa ttactttctt cttcaactgt
     1021 caaagtaacc gaaggaattg ggttttcaat aaaaaaagtt gcttgttgaa ttttattttc
     1081 tactttttgt tgaacagctt tttctactaa taaggtttca tttgcaattt tcttttcata
     1141 ccattggttt ccaaaaaatc ctgccatacc aataccaaaa gcaaaaatag ccgcatattt
     1201 catccacgaa gaagttcttt tttcaggagt gaaaagaatg ggtgtttttt cttctaattt
     1261 ttcaacttcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tcttttttca
     1381 ggagtgaaaa gaatgggtgt tttttcttct aatttttcaa cttctttttt ataaacttct
     1441 cgtttaattt ttggagaaat aaaagaggtt aagccaaaag aatccgttgc ataatttaaa
     1501 ttgtcaattg gttcaaaaac caaactattt tcactgttta gtgatagttt tccaatattt
     1561 ttaagcgtca gtgcttcact tttttgaatt gaaaatttcc acgcacttac ttcttcttca
     1621 atcaaggcaa cagctttgtc gtaatctatc ttttcacaaa gtgaaatgtg atttgctaga
     1681 agaccatcat tgtttttcaa atgaaaatta aatgacaata cttttttagg aggataaaat
     1741 gtattagtat cctgaactaa ttgagcagaa cggttttcag ttaaaaaagc accaaatcct
     1801 ggcaccgtta cacattggta gcggtacaat aattgggaaa tgtgttgttc tattctcatc
     1861 acaacaaaga aaaaatattc aactcccgaa tcaaaatttt acacacaatt tttattaaca
     1921 atttgccaaa atttgataac tgtttaatgg tcatgacatt aaattttatt ctttttaaat
     1981 tgaaatagga tttaataaat ctgggagaaa aaatttaaca aaaaagcttt taaaacacac
     2041 aaaaaatatg tttctttgat tttcatttat acgccatgac gacatcagaa ttatatttta
     2101 cgctcgcgtt actgcaagta gaaggagtag gtgacatagt tgccaaaaaa ctcatcaacc
     2161 attgtggctc tgccgaaaaa gtgtttgaca ccaaaaagaa tcgattgtta tccattgacg
     2221 gaattggtga aattttatta aaaaatcttt ccgataaaag tgtttttaag ttggctcaac
     2281 aagaaatcga ctttgttgaa caagaaaaaa tagcttttca ttctttttta gacgaaaaat
     2341 atccggatcg attaaaacat tgtattgatg gcccgccaat tattttttcg tcgggaaata
     2401 tagatttgca agctaaacga attattagca tagttggtac gcgaaaaata acaagctacg
     2461 gaacagaatt ttgcaagaaa cttattgaag atttagctcc attgaaccca gttattgtga
     2521 gtggttttgc ttatggtgta gatattatgg ctcaccaagt tgcaatggaa cataacctgc
     2581 aaacgatagg tgttttagct cacggactaa accaagtata tcccaaaact cataaaaagt
     2641 acatttcaaa gatggaacaa aatggcggat ttttaacgga attttggagc acttctaatc
     2701 ctgaaaaaga aaattttgtc aaaagaaacc gaattgtcgc aggcatgtca gaagcaacta
     2761 ttgttattga aagtgccgat agaggaggct cattaataac agctacgtta gccaatgatt
     2821 ataaccgcga tgtttttgca gttcccggaa gaataactga taaatacagt cagggatgta
     2881 ataatttaat taaaacacaa cgtgcacatt tgctttcctc tgcggctgat ttagtatata
     2941 ttttaaattg ggaattggag aataaaaaga ctaaaacaat tcaaaaaca
//
LOCUS       DAOTCW010000213         2987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_27270_length_2987_cov_45.408254, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000213 DAOTCW010000000
VERSION     DAOTCW010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27270_length_2987_cov_45.408254"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..647)
                     /locus_tag="P5335_11465"
     CDS             complement(18..647)
                     /locus_tag="P5335_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRZ75543.1"
                     /translation="MIKVCLADNQPVVHFGVKSYFKDHAEISVVGHVGNYNMILDMLK
                     IKPVDILVLDLELEGLTSINLVKYILKEFPSTKIIIFSNLSENIYAPNSLKAGVSAYL
                     HKTSKLETLGNVIVKVHNGAIIFNDTIKKNLALIAKQNKSERLYRKLSNREIEVLRFL
                     SDGKKNNEISKILGLNEKTISTYKLRLLTKLNVTNLVDLVNKAKTLEIV"
     gene            complement(758..1570)
                     /gene="nadE"
                     /locus_tag="P5335_11470"
     CDS             complement(758..1570)
                     /gene="nadE"
                     /locus_tag="P5335_11470"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRZ75544.1"
                     /translation="MQKNTSIHTEKVNEHIVHWLKNYALNAKVNGFVVGVSGGIDSAV
                     TSTLCAQTGLKTLCVELPIHQASSHVSRKKEHIAQLKKRFSNVDSISIDLSETFEHFK
                     KTVPFSENEAKFNLSLANTRARLRMTTLYYLAGIHGLIVAGTGNKVEDFGVGFYTKYG
                     DGGVDLSPIADLMKSEVYALGHYLKVPDSILNATPTDGLFGDHRSDEDQLGASYDELE
                     QAMNAVEAGKTAADFKGRNLEVFNIYNRLNSANKHKMNPIPVCKIPNELRQI"
     gene            1692..2648
                     /gene="gldB"
                     /locus_tag="P5335_11475"
     CDS             1692..2648
                     /gene="gldB"
                     /locus_tag="P5335_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldB"
                     /protein_id="HRZ75545.1"
                     /translation="MKKYLLIALLIFFISCDKKSKVEKEVEEIPLSLTIERFDKVFYE
                     TPISDFQQMRKKYAAFFPTQYPDSVFINKIKNPLYRELYDEVQKKYANFQPVQTEIED
                     VFRHIKYYFPTQKLPQKVTTVISEMDYQNKVIYTDSLLIISLDLYLGKEHKFYEFPDY
                     FRQTFERSQIMPDIVTDFSTRVIAQPRDKSFLAQMIYFGKEQYLKDLLLPSVEDFDKM
                     GYSKEQQIWCEENEDEIWRYFIEDNLLYDSDSKLIQRFIAPAPFSKFYLEIDNESPGR
                     TGVWIGWQIVRSFMKNNEVSLADLMAMDAKEIFTRSKYKPKK"
     gene            2648..2980
                     /gene="gldC"
                     /locus_tag="P5335_11480"
     CDS             2648..2980
                     /gene="gldC"
                     /locus_tag="P5335_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HRZ75546.1"
                     /translation="MANSKYEIKFDIELDDNKIPEKMHWTAKDGGVVNQETKAIMLSV
                     WDAKSKESLRIDLWTKEMPVDEMKVFFHHTLLAMADSLQKATGEDKMGDTMRDFCDYF
                     AEKLELKK"
BASE COUNT          994 a          470 c          491 g         1032 t
ORIGIN      
        1 tttttttcta agtattttta tacaatctcc agtgtttttg ctttgttaac taagtcaacc
       61 aagttagtca cattcaattt agtgagcaat cgcaatttat aggtactgat cgttttttca
      121 ttcaatccta aaattttaga tatctcgttg ttctttttac catcgcttaa aaagcggaga
      181 acttcaattt cacggttaga aagtttgcga tacagacgtt cacttttatt ttgcttagca
      241 attaaagcca ggtttttctt aatggtatca ttaaatataa tggctccatt atgaactttc
      301 acaatcacat tgccaagggt ttctaatttg gatgttttgt gtaaataagc agaaacacct
      361 gcttttaaag agtttggagc ataaatattt tcagataaat tgctgaaaat aataattttt
      421 gtgctcggaa attcttttaa aatgtatttg accagattaa tactggttaa accctctaac
      481 tctaagtcta aaactaagat gtcgacaggt ttaattttga gcatgtctaa aatcatgttg
      541 taattaccaa cgtggcctac aacacttatt tctgcatggt ctttaaagta cgatttcacc
      601 ccgaaatgta caacgggttg gttatcggct aaacaaactt taatcataat aagtggtatt
      661 ttggttgaaa aatgattatc aattatttag ggcagttaaa gatactacat ttttttctaa
      721 tggaatgtat ttctgacaaa aaaaattaag aaaaatttta gatttgtctt aattcattgg
      781 gaattttgca taccggtata gggttcattt tgtgtttatt agcagaattt agccgattat
      841 agatgttaaa aacctctaaa tttcttcctt taaaatcagc ggccgttttt cctgcttcaa
      901 cagcattcat ggcttgttct aattcgtcat aacttgctcc taattggtct tcatcacttc
      961 tgtgatctcc aaataatcca tcggttggag tagcatttaa aatggaatcc ggaactttta
     1021 aatagtgccc taaagcatat acctctgact tcattaaatc tgcaatcgga cttaaatcta
     1081 ctcctccatc accatatttt gtgtaaaaac caacgccaaa atcttctact ttatttccgg
     1141 ttccggccac tatcaaacca tgaatacctg ctaaataata tagggttgtc atccgtaatc
     1201 ttgctctggt attggcaagc gacaaattaa atttcgcttc gttttctgaa aaaggaaccg
     1261 ttttcttaaa gtgctcaaag gtttctgata aatcaatact tatgctgtca acatttgaaa
     1321 aacgcttttt taattgagct atgtgttcct tttttcgact tacatgtgaa gaggcttgat
     1381 gaataggtaa ttcaacacac aaagtcttta atccggtttg agcacacagg gtggaagtaa
     1441 cggcactgtc tattccgccg gaaactccca caacaaatcc gtttactttt gcgtttaagg
     1501 catagttttt tagccaatgc acaatgtgct catttacttt ttctgtgtga atagaagtat
     1561 ttttttgtat catattttta agttatacca tttggtgcag tatatttgca aaccaaaatt
     1621 agtacgtaaa aataataaaa accatttgat ttttcaggac gaattaatct tatcataatg
     1681 caactcatag aatgaaaaaa tatcttttaa ttgccttgct cattttcttc atttcatgtg
     1741 ataaaaaatc aaaagttgaa aaagaggttg aagaaattcc gcttagcttg actattgaac
     1801 gttttgataa agtgttttat gaaacaccta tttccgattt tcaacaaatg agaaaaaaat
     1861 atgctgcttt tttcccaaca caatatccgg atagtgtttt tataaataaa attaaaaatc
     1921 cgttgtaccg tgagttgtat gatgaagttc aaaaaaaata cgcaaacttt caaccggttc
     1981 agactgaaat tgaagatgtt ttccgtcata taaaatatta ctttcccact caaaaattac
     2041 ctcaaaaagt gaccactgtg atttctgaaa tggattatca aaataaagtt atttataccg
     2101 attctttatt aattatttca ttagatttat atttggggaa ggagcataaa ttttatgaat
     2161 ttcctgatta tttccggcaa acgtttgaac gttctcaaat tatgcctgac atagttactg
     2221 atttttcaac ccgagtaatt gcccaaccta gagataaaag ctttttagct caaatgattt
     2281 atttcggaaa agagcaatat ttaaaggatt tattacttcc gagcgttgaa gattttgaca
     2341 aaatgggcta ctcaaaagaa caacaaattt ggtgtgaaga aaatgaagat gaaatttggc
     2401 gttattttat cgaagacaat cttttatatg attctgacag taaattaatt cagcgattta
     2461 ttgcaccggc acctttttct aaattttatc tcgaaattga taatgaatct ccaggaagaa
     2521 ccggagtttg gattggatgg caaattgtgc gttcttttat gaaaaataat gaagtgtctt
     2581 tagcggattt aatggcgatg gatgccaaag aaatattcac acgatcaaaa tataaaccaa
     2641 aaaaataatg gcaaatagta aatacgaaat caaatttgat attgaattag acgataataa
     2701 aatccctgaa aaaatgcatt ggactgccaa agatggcgga gtagtaaacc aagaaacaaa
     2761 agcgattatg ttatcggttt gggatgctaa atctaaagaa tcgttacgga ttgatttatg
     2821 gacaaaagaa atgcctgtag atgaaatgaa agtttttttt catcatacac ttttggcaat
     2881 ggctgattct ttgcaaaaag ctaccggaga ggataaaatg ggtgatacaa tgcgtgattt
     2941 ttgtgattat tttgctgaaa aattggaatt gaaaaaataa agagtag
//
LOCUS       DAOTCW010000214         2974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_27437_length_2974_cov_31.533744, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000214 DAOTCW010000000
VERSION     DAOTCW010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27437_length_2974_cov_31.533744"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="P5335_11485"
     CDS             complement(<1..693)
                     /locus_tag="P5335_11485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRZ75547.1"
                     /translation="MQLSIIILNYNVRFFLEQCVLSVQAALQHIDGEIIVVDNNSSDA
                     SCQMMKTRFPNIKFIENKKNTGFPKGNNIGVAEAKGEYICIINPDTVVAEDTFEKILN
                     TNNWQLNTGILGCKLIDGTGKFLPESKRGIPTPWVAFTKIFGLYKIFPKFRMFNQYYA
                     QHLTENQSGNVEILVGAFMVMKRDLYLEVGGFDENCFMYSDDIDLSYRLLKSGKINYY
                     FCETSVIHYKGES"
     gene            792..2651
                     /locus_tag="P5335_11490"
     CDS             792..2651
                     /locus_tag="P5335_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRZ75548.1"
                     /translation="MNYLSVENISKSYGERVLFENISFGINKDQKIAFIAKNGSGKTT
                     IMNIINGFDEPDTGQVVVRKNIRMSFLSQKPNLQDELTIEESIFASDNETLKVIEQYE
                     KALENPENEEAYQKAFDLMDQHNAWDFETQFKQILFKLKLEDFKLKVKSLSGGQKKRL
                     SLAIILINRPDLLILDEPTNHLDLEMIEWLESYFAKENITLFMVTHDRFFLERVCNEI
                     IELDNGKLYQYKGNYSYYLEKKEERIASENASIDKAQNLFVKELAWMRRQPKARTTKS
                     KSRQDDFYVIKEKAESRRKENVVELEINMERMGSKIIELHKLFKKFKERVILDNFSYD
                     FQRGERIGIIGKNGTGKSTFLNILTKTILPDAGKVVIGDTIKVGYYTQSGITPKPGQK
                     VIDIIKEYGEYIPLTKGKIISASQLLERFLFDAKKQYDFVEKLSGGELKRLYLCTVLI
                     QNPNFLILDEPTNDLDILTLNVLESFLLDFPGCLLVVSHDRYFMDKIVDHLFVFRGDG
                     EIEDFPGNYSDFRAYEDSADVALKEENKADKKAWKQNNSTGNLSFNEQKEFQKIEREI
                     KDLEFDKKQIEQLFADGKVVDDAIEKKANELHVIIKKLEEKEERWFELSSKIE"
     gene            2732..>2974
                     /locus_tag="P5335_11495"
     CDS             2732..>2974
                     /locus_tag="P5335_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRZ75549.1"
                     /translation="MFHIIKSYLKFLQHSKNQHGVHSPFVFDLVTKCFYDKTKYPEYE
                     VLKEYRKSLLANENIIEVTDFGAGSRVFKSNQRKISA"
BASE COUNT         1060 a          439 c          552 g          923 t
ORIGIN      
        1 gctttcacct ttgtagtgta tcactgaagt ttcgcaaaaa taatagttta ttttaccgga
       61 tttcaacaag cggtaactca aatcaatatc gtcggaatac ataaaacagt tctcatcaaa
      121 tccgccaact tcaaggtata aatcacgttt catgaccata aaagctccaa ctaaaatttc
      181 aacatttccg gattgatttt cggttagatg ttgagcataa tattgattaa acattcgaaa
      241 ttttggaaaa attttataca acccgaagat ttttgtaaat gccacccaag gagttggaat
      301 accacgttta ctttcgggta gaaattttcc ggtgccatcg atgagtttgc acccgagtat
      361 tccagtgttc agttgccagt tattagtatt aagtattttc tcaaatgtat cttctgcgac
      421 tactgtatca gggttaataa tacaaatgta ttcgcctttt gcctcggcta ctccaatgtt
      481 atttcctttt ggaaaaccgg tatttttttt attttcaatg aatttgatgt tcggaaaacg
      541 ggttttcatc atctgacaac ttgcatctga cgaattatta tctaccacaa tgatttcgcc
      601 gtcaatatgt tgtaaagctg cctgaacact aagcacacat tgttcgagaa aaaaacgaac
      661 gttgtaatta aggatgatga tggaaagttg catgggacaa atataatggg aaaatttgtc
      721 aaattgaaaa actgacaaat tgaaaaactt tacaatcttt aaaatagtac ttttgcaaaa
      781 aaaatattct tgtgaattac ctctctgtag aaaacatatc aaaatcatat ggtgaacgcg
      841 tgttgtttga aaatatatct ttcggtatca acaaagacca aaaaatagct ttcatcgcca
      901 aaaatggttc aggaaaaaca accattatga acattattaa tggctttgac gaaccggata
      961 cgggtcaagt ggtggttcgt aaaaacatcc gaatgtcttt tttgtcgcaa aaaccaaatt
     1021 tgcaagatga gttgacgatt gaagaaagca tttttgcatc tgataacgaa acactgaaag
     1081 ttattgagca atatgaaaaa gctttagaaa acccggaaaa tgaagaagcc tatcaaaaag
     1141 cgtttgattt gatggaccaa cacaatgcat gggattttga aacgcaattc aaacagattt
     1201 tgtttaaatt gaaattggaa gattttaaat tgaaagtcaa aagtctttct ggtggacaaa
     1261 aaaagcgtct ttcgttagcc attattttga ttaatcgacc agatttattg attttggatg
     1321 agccaactaa ccacttagat ttggaaatga ttgaatggtt ggaaagttat tttgctaaag
     1381 aaaatataac gttgtttatg gtgacgcatg accgtttctt tttggaacgc gtttgcaatg
     1441 aaattatcga attggacaac ggtaaactat accaatacaa aggaaattac tcctattatc
     1501 tagagaaaaa agaagagcga attgcttcgg aaaatgcaag tattgataaa gctcaaaatc
     1561 tttttgtaaa agaattggct tggatgcgtc gccaaccaaa agcgagaact acgaaatcga
     1621 aatcacgtca agatgatttt tatgtgatta aggaaaaagc cgaaagtcgc cgaaaagaaa
     1681 atgtggtaga gttggaaatc aatatggaac gcatgggaag caaaattatt gagttgcata
     1741 aactattcaa aaaattcaaa gaacgtgtga ttttggataa tttctcctat gattttcaac
     1801 gtggcgaacg cattggaatt atcggtaaaa acggaaccgg aaaatcgact tttttgaata
     1861 ttttaaccaa aactatttta cctgatgcag gaaaagtggt gattggtgat acgattaagg
     1921 taggttatta tactcaaagc ggcatcaccc caaaaccggg tcagaaagta attgatatta
     1981 ttaaagaata cggcgaatat attccgctca cgaaaggaaa aatcatttcg gcttcgcaat
     2041 tgttagaacg ttttcttttt gatgccaaaa aacaatatga ttttgtagaa aaattgagtg
     2101 gtggcgaatt gaaacgcttg tatttatgta cggttttgat tcagaatccg aactttttga
     2161 ttttggatga gccaacgaat gatttggata ttttaacgtt gaatgttttg gaaagttttc
     2221 tattggattt tccgggttgt ttattagtgg tttcacacga tagatatttc atggataaaa
     2281 ttgttgatca cttatttgtt tttcgtggtg atggtgaaat tgaagatttt ccgggtaatt
     2341 attccgattt cagagcctat gaagatagtg ccgatgtagc cttgaaagag gaaaataaag
     2401 ctgataaaaa agcgtggaaa caaaataatt cgacaggaaa tttatcattt aacgaacaaa
     2461 aagaatttca aaaaattgaa cgtgaaatca aggatttgga atttgataaa aagcaaatcg
     2521 aacaattgtt tgccgatgga aaagtagtcg atgatgcaat tgagaaaaag gcaaatgagt
     2581 tgcacgtaat tataaaaaaa ttagaagaaa aggaagagag atggtttgaa ctttcttcga
     2641 aaattgagta actatttaaa ccatcaagtt attaagttac attaaggctt cttaattccc
     2701 ttaatacctt aatggtttaa aaaaaataaa aatgtttcat ataataaagt cctatttaaa
     2761 gtttctacaa cattccaaaa atcaacacgg agtgcactct ccatttgtat ttgatttggt
     2821 tacgaaatgt ttttatgata aaaccaaata tcccgaatac gaagttttaa aagaataccg
     2881 aaaatccctt ctagccaacg aaaatataat tgaagtaacc gattttggtg ccggttcaag
     2941 agtttttaaa tccaatcaaa gaaaaatttc tgct
//
LOCUS       DAOTCW010000215         2969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_27508_length_2969_cov_38.913178, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000215 DAOTCW010000000
VERSION     DAOTCW010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27508_length_2969_cov_38.913178"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="P5335_11500"
     CDS             <1..626
                     /locus_tag="P5335_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRZ75550.1"
                     /translation="SKQDISKSLNALLNDVNGKKEQNLVDTLTIRSMSKAVYSTENIG
                     HYGLAFDYYSHFTSPIRRYPDVMVHRLLQYYLDGGKSADADVFEEKCAHSSDMENLAA
                     QAERDSIKYMQVKYMQDHKDQEFLGVISGVTEWGIYVEIVENKCEGMVRIREIKEDYY
                     VFDEKQYALVGEVSKSILQLGDEVYVKVKNADLVKKQLDFHFIRRNE"
     gene            701..1372
                     /locus_tag="P5335_11505"
     CDS             701..1372
                     /locus_tag="P5335_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRZ75551.1"
                     /translation="MKNCLFILLVFINTTLFSQTITKNPGEFTSVKVFDQISVQLIPG
                     TESKVEIKGTRASEVELVNKNEELKIRMKFSKLLQGESIEVTVYYQKLESVEASEGSY
                     VNSETPIKGINFSLNAKEGAEIKIALDVQRATVKLNSGGKIKISGTTDNQEVMITSGG
                     ELKASNFISKQSTVSVSAGGEASIHATDFVDAKVKAGGEIYIYGSPKQINQKTVIGGT
                     IKEMN"
     gene            1458..2141
                     /locus_tag="P5335_11510"
     CDS             1458..2141
                     /locus_tag="P5335_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRZ75552.1"
                     /translation="MLNDILAAIPWGILLAFTIGPVFFVLLETSITKGFKAAMVFDLG
                     VVFGDFIFILIAYFSTNQLLEKLKDDPSLFIFGGVIMFFFGLISFIKEKKSKKKNLNQ
                     EFHADSIPKNNYLSLFFKGFILNFINIGVLGFWVGIIIVFGPQMNMEPNRITLFLSVI
                     ITTYLLTDIVKIVLAKQLKNRLTPYIIYKIKRVISAVLMIFGFFLIIQGFFPKEKEMI
                     KEKIEQMNE"
     gene            2147..2797
                     /locus_tag="P5335_11515"
     CDS             2147..2797
                     /locus_tag="P5335_11515"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HRZ75553.1"
                     /translation="MKTVQFICCLLLSQFLLAQSDTSFVSKPKLGLISVDKLNVVYRG
                     IRNPISIAVPNAKSFTVSGLGVSQEKGNYYISPGQGNEMIVTLEIVLEDDSKVIEEHV
                     FRIKNIGNPLGRINNRNCKDCIIIIQKLELQNAEISVYFEDLLTGIKLNVSSFSIELS
                     SKTNLEISGNKIDEETYKQLIRQKNNSEFKIHNIYYTESLLGCFPSAGVINVRISD"
BASE COUNT         1082 a          375 c          520 g          992 t
ORIGIN      
        1 aatcaaaaca agatatttct aaatcattga atgctttatt gaatgatgtg aatggaaaaa
       61 aagaacagaa tttagtagat accttgacca tacgaagtat gagtaaagct gtttattcta
      121 cggaaaatat tggtcattac ggattggcat ttgattacta ttcgcatttt acgtcgccta
      181 ttcgtcgata tcccgatgta atggtgcatc gtttgttgca atattattta gatggtggaa
      241 aatcggccga tgcggatgtt tttgaagaaa aatgtgctca ttcatccgac atggaaaact
      301 tagcagctca agccgaacgt gacagtatca aatatatgca ggtgaaatat atgcaagacc
      361 ataaagacca ggagttttta ggagtaattt caggtgtaac tgaatgggga atttacgtag
      421 aaattgtaga gaataaatgt gaaggaatgg ttcgaattcg tgaaatcaaa gaagattatt
      481 atgtattcga cgaaaaacaa tatgctttag taggtgaagt ttctaagagt attttacaat
      541 taggagacga agtttacgtt aaagtaaaaa atgcagactt agtgaagaaa caattggatt
      601 ttcattttat aagaaggaat gaataatacc atttcttttt aaaactgtaa cattttgaca
      661 ctggtaaagt ctttgatatt aacttttaat tttaatcgtt atgaaaaatt gtctatttat
      721 ccttttagta tttataaata caactttatt ctctcaaaca ataactaaaa atccgggaga
      781 attcacttca gtaaaagtat ttgatcagat atctgttcaa ttgattccag gtacggaaag
      841 taaagtagaa atcaaaggaa cgcgagcttc agaagttgaa ttagtcaaca aaaatgaaga
      901 attaaaaatt cgaatgaaat tttcaaaact tttacaagga gaatcaatag aagtaacggt
      961 ttactaccaa aaattagaat ctgttgaagc cagcgaggga tcgtatgtaa atagtgaaac
     1021 accaattaaa ggaataaatt ttagtttaaa tgctaaagaa ggtgcagaaa ttaagattgc
     1081 tttagacgtt cagcgagcta ctgtaaaatt gaattccggt ggtaaaatta aaatttccgg
     1141 tacaacagat aatcaagaag ttatgataac ctccggaggt gaattaaagg cttctaattt
     1201 tatcagtaaa caatcaacag ttagtgttag tgccggtggt gaagcatcaa ttcacgcaac
     1261 agattttgtt gatgctaaag tgaaagccgg cggagaaatt tatatttatg gaagtccgaa
     1321 acaaattaac caaaaaacag taattggagg aaccattaaa gaaatgaact aaaaatacgt
     1381 ttattttaaa ttttaaattt taaattagca tttagaaagt ttcacaactt gaaacttaaa
     1441 acttgaaaca aacttaaatg cttaacgata ttttagcagc tattccgtgg ggaattttac
     1501 ttgcttttac tattggccct gttttttttg ttttacttga aacaagcatt accaaaggat
     1561 ttaaagcagc tatggttttt gatcttgggg tggtttttgg tgattttata tttattttaa
     1621 ttgcttattt cagtacaaat caactgttag aaaaattaaa agatgacccg agtttattca
     1681 tttttggagg tgttatcatg ttttttttcg gattaatttc ttttataaaa gaaaaaaaaa
     1741 gtaaaaagaa aaacctcaac caggaatttc atgctgattc gatacctaaa aacaattacc
     1801 tcagtttatt ttttaaagga tttattctca attttatcaa tattggtgtt ttaggttttt
     1861 gggttggaat tattattgtt tttggcccgc aaatgaatat ggaaccaaac agaataacac
     1921 tttttttatc cgttattatt acgacttatt tactgacaga tatagtaaaa atcgttttgg
     1981 ctaaacaatt aaaaaatcgt ttaacgcctt atattatcta caaaattaaa cgagtgatta
     2041 gtgctgtttt aatgattttt ggtttctttt tgattattca aggatttttt ccaaaggaaa
     2101 aggaaatgat taaagaaaaa attgagcaaa tgaatgaata aaagctatga aaacagttca
     2161 gtttatatgt tgtttacttt tatctcagtt tttattagca caaagtgata cttcattcgt
     2221 ttcaaaaccc aaattaggct taatatccgt tgataaatta aatgtggttt atcgaggaat
     2281 tagaaatcca atttcgattg ctgtacccaa tgcaaaatca ttcactgttt ctggattagg
     2341 agtatcccaa gaaaaaggaa actattatat ttcaccagga caagggaacg aaatgattgt
     2401 gactttagaa attgttttag aagatgattc gaaagtaatt gaagaacatg tttttaggat
     2461 aaaaaatatt ggaaaccctc taggtagaat aaataacagg aattgcaagg actgtattat
     2521 tattatacaa aaattagagt tgcaaaatgc tgaaatttcg gtttattttg aagatctatt
     2581 aaccggtata aagttgaatg tatcaagttt cagtattgag ctttctagta aaacaaattt
     2641 agaaatatct ggaaacaaaa ttgatgaaga aacatataaa cagcttataa ggcaaaaaaa
     2701 taactctgag tttaaaattc ataatatata ctatactgaa agtttattag gctgttttcc
     2761 aagtgccggt gttattaatg ttagaatttc tgattgattc ttattaaaac gaatcgtagt
     2821 aaaaaaaaat ctgtttttga gatatcgccc tcccgattgc taacgggaga atctgggcag
     2881 acggttttgc attatcttta attaattttt tctaaaaatt tagcataaaa aaacctccaa
     2941 gtttccttga aggtttcaga ggcatcgcc
//
LOCUS       DAOTCW010000216         2965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_27574_length_2965_cov_54.333333, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000216 DAOTCW010000000
VERSION     DAOTCW010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27574_length_2965_cov_54.333333"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1496
                     /locus_tag="P5335_11520"
     CDS             117..1496
                     /locus_tag="P5335_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HRZ75554.1"
                     /translation="MQLYNTMRAEERAELIDQAGKQRLTLSFYAYAKIEDPKKFRDDL
                     FLAWNPLDVLGRIYVANEGINAQLSLPADYFYQFKDTIEAYDFMKGIRLNIAREHDDH
                     SFLKLTIKVRHKIVADGLDDSTFDVTNKGIHLKAKEFNQLLEDPNTIVVDFRNHYESE
                     IGHFKGAITPDVDTFRESLPIIEEQLKDFKEDKNLLMYCTGGIRCEKASAFFKHKGFK
                     NVYQLEGGIIEYARQIEQEGLQSKFIGKNFVFDHRLGERITNDIVSQCHQCGKPCDNH
                     TNCVNDGCHLLFIQCDECKEKMDNCCSAECQEIIHLPLAEQVKLRRGIQKGNMIFKKG
                     KSDALKFKHSGELSDVSLAIAEPGVNELAKQKPFRERIKRKKVLVAKAQHYYTNAQVG
                     LFLMENHELVVGDSILISGPTTGNEEFVLEKMFVNGTENSLAKPGDKVTFNVPFRIRL
                     SDKLYKILS"
     gene            1499..2740
                     /locus_tag="P5335_11525"
     CDS             1499..2740
                     /locus_tag="P5335_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HRZ75555.1"
                     /translation="MTLSGKIELMAPAGNFESLQAALDNGADSIYFGVEQLNMRARAT
                     INFTMEDLKEIAKRCNAKNVRTYLTLNTIIYDHDISVVKTLLNKAKEAGITAVIASDQ
                     AVIASARAIGMEVHISTQLNVTNVETVKFYSLFADTIVLSRELSLRQVKKITEQIEKE
                     QIKGPSGNLVEIEIFGHGALCMAVSGKCYLSLHSHNSSANRGACKQNCRKKYTVIDQE
                     SGFEIEIDNEYMMSPKDLCTLDFLDQVIDSGIKVLKLEGRGRAPEYVAMVTKTYRDAI
                     DSYYDDTFTKEKIDRWMEDLSTVYNRGFWSGYYLGQKLGEWSTNPGSNATQKKVYVGK
                     GVHFFTKSNIAEFKIEAYDIKIGDKIMINGPTTGVQELVIEDMMVNDLKLEKASKGDS
                     CTLKLPFRVRNSDKLYKIVEQ"
     gene            2742..>2965
                     /locus_tag="P5335_11530"
     CDS             2742..>2965
                     /locus_tag="P5335_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRZ75556.1"
                     /translation="MVIITLQRDKCIGCNYCVEMAPAQFQMSKKDGKSVLLKSINNKG
                     FHTLKSPNHVIVEACEAAEKACPVHIISVK"
BASE COUNT         1032 a          503 c          572 g          858 t
ORIGIN      
        1 tcaaaaaaat agtttgttaa gattgatttt gttaactcat tcaatttagt acctttgcca
       61 acgaattctt ccatttagaa ttcaaaccta ttttactcac tcaaactgtt tatagcatgc
      121 aactgtataa cacaatgcga gcagaggaaa gagccgaact tattgatcaa gccggtaaac
      181 aacgtcttac gttgtctttc tatgcctacg ccaaaattga agaccctaaa aaatttagag
      241 acgatttgtt tttagcctgg aatccgttgg atgtcttggg aagaatttac gtggcgaatg
      301 aaggaatcaa tgcccaactt tcattacccg cagattattt ttatcaattt aaagatacga
      361 ttgaagcgta tgattttatg aaaggtattc gtttgaatat cgccagagaa cacgacgatc
      421 attctttttt aaaactgact attaaagtta gacataaaat tgtcgcagat ggtcttgatg
      481 attctacttt tgatgtaacc aacaagggaa ttcacctaaa agcaaaagaa ttcaatcaat
      541 tacttgaaga ccctaacacg attgtggttg atttcagaaa tcattacgaa agtgaaattg
      601 gtcattttaa aggagcaatt acgccggatg tggacacctt tcgtgaatct cttcctatta
      661 tcgaagaaca actaaaagac ttcaaagaag ataaaaattt attgatgtat tgcaccggag
      721 gaattcgatg cgaaaaagca agtgcgtttt ttaaacacaa aggatttaaa aacgtgtatc
      781 aattagaagg cggaatcatt gaatatgccc gtcagattga gcaagaaggt ttgcaaagta
      841 aattcatcgg taaaaatttt gtgttcgatc atcgtttggg cgaacgaatc actaacgata
      901 ttgtttcaca atgccatcaa tgtgggaaac cctgcgacaa tcataccaat tgtgtaaacg
      961 atggatgtca tttacttttt attcaatgcg acgaatgcaa agaaaaaatg gataattgtt
     1021 gttcagccga atgtcaggaa attattcatt taccattagc cgaacaagta aaattaagaa
     1081 gaggcattca aaaaggcaac atgattttca aaaaaggaaa atcggatgct ttaaaattca
     1141 aacattccgg cgaattgtcc gatgtttctt tggcaattgc agaacccggc gttaacgaac
     1201 tggcaaagca aaaacccttt cgcgaacgca ttaaaagaaa aaaagtattg gttgcgaaag
     1261 ctcaacatta ctataccaac gcccaagttg gactgttttt gatggaaaac catgaattag
     1321 ttgttggtga cagcatttta atttccggac caactaccgg aaatgaagaa tttgtgttag
     1381 aaaaaatgtt tgttaacgga accgaaaatt ctttagcaaa accgggagat aaggtgactt
     1441 ttaatgtacc atttcgcatt cgattatctg ataaacttta taaaatcctt tcgtaatcat
     1501 gactttatca ggaaaaatag agcttatggc tcctgccgga aactttgaat ccttacaagc
     1561 tgctttggat aatggtgccg attcgattta ctttggagta gaacaattaa atatgcgagc
     1621 tcgggcaacc atcaatttca cgatggagga tttgaaagaa attgccaaac gttgcaacgc
     1681 caaaaatgtt cgaacctatc ttactttaaa tacaattatt tatgaccacg atatttcagt
     1741 ggtaaaaacc cttttgaaca aagcaaaaga agcaggaatc acagcggtaa ttgcctcaga
     1801 tcaggcagtg attgcctcag caagagctat cggaatggaa gtacacattt caacccaatt
     1861 gaatgtaaca aatgtggaaa cagtgaaatt ttacagcctt tttgctgata ccattgtttt
     1921 atctcgtgaa ttaagtttgc gtcaagtaaa aaaaatcaca gagcaaattg aaaaagaaca
     1981 aatcaaagga ccaagtggca atttagttga aatcgaaatt tttggtcatg gagccttgtg
     2041 tatggctgtt tccggaaagt gttatttgag tttacattcg cataattcct ccgctaaccg
     2101 tggagcttgt aaacaaaatt gccgaaaaaa atataccgtt atcgaccaag aatccggttt
     2161 tgaaattgaa atcgataacg aatacatgat gtctcccaaa gatttgtgca ccttagattt
     2221 tttagaccaa gtaatcgatt cgggtattaa agttttgaaa ttagaaggcc gagggcgtgc
     2281 tcctgaatat gtggcaatgg tcaccaaaac ctatcgcgac gctattgatt catactatga
     2341 cgatacattc accaaagaaa aaatcgaccg atggatggaa gatttaagta cagtatacaa
     2401 ccgtggtttt tggtccggtt attatttagg acaaaaatta ggcgaatgga gtactaatcc
     2461 gggttcaaat gcgacgcaaa agaaagtata tgtaggcaaa ggtgttcact tttttacaaa
     2521 atcgaatata gccgagttta aaattgaagc ttacgacatc aaaattggtg ataaaattat
     2581 gattaacgga cccacaaccg gtgttcagga attagtaatt gaagacatga tggtaaacga
     2641 tttaaagctg gaaaaagcat ccaaaggcga cagttgcacc ttaaaacttc cgtttagagt
     2701 tcgaaattca gataaattat ataaaattgt ggagcaataa tatggtcatt attacccttc
     2761 aacgcgacaa atgtattggt tgcaattatt gtgttgaaat ggcacctgcc caatttcaaa
     2821 tgtctaaaaa agatggcaaa tcggtgttat taaaatcaat caataacaaa ggttttcaca
     2881 ccttaaaatc gcctaatcat gtgattgtag aagcttgtga agctgccgaa aaggcttgtc
     2941 cggttcatat tatttcggta aaaga
//
LOCUS       DAOTCW010000217         2958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_27672_length_2958_cov_34.353772, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000217 DAOTCW010000000
VERSION     DAOTCW010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27672_length_2958_cov_34.353772"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /gene="gltX"
                     /locus_tag="P5335_11535"
     CDS             complement(<1..1483)
                     /gene="gltX"
                     /locus_tag="P5335_11535"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRZ75557.1"
                     /translation="MSKPVRVRFAPSPTGPLHIGGVRTALFNYLFAKKHNGVFYLRIE
                     DTDQNRFVPGAEQYIFEALEWLGISPSETVGKNEKFGPYRQSERKHLYKQYADQLINS
                     GWAYYAFDTAEALDLQRKQHEEQGKTFIYNWHNREKLDTSLVMSTEETNRRIEAGEDF
                     VIRFKTPVNEILELKDIVRGDIKFDTNLLDDKVLFKSDGMPTYHLANIVDDHLMETSH
                     VIRGEEWLPSLPLHHLLYKAFGWEAPEFAHLPLILKPIGNGKLSKRDGDKMGFPVFPL
                     EWKTEEGISSGYREKGYFPEAVINFLALLGWNDGTDQELFTLEELAEKFDLNRVHKAG
                     AKFDPEKNKWFNHQYLQKQNEEELADIFSSILKEKGISVENYKLIKIVSSIKERANFV
                     SDFWDLADYFFVEPTSYDEKASKNWKEETPTLMKQVIEVLESIEDFTSLNIETILKDW
                     MTVNEIGMGKVMQPLRLSMVGALKGPHLFDIIELIGKEETIKRL"
     gene            1650..>2958
                     /locus_tag="P5335_11540"
     CDS             1650..>2958
                     /locus_tag="P5335_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75558.1"
                     /translation="MLEIKSLIYQKLEVFIKKYYTNELLKGLIFFVGLGLIYMFFTLF
                     IEYFLWLKPTGRTILFWLFILVELFLLARFILFPLFKLIKLQKGIDYTQASKIIGNHF
                     TEVSDKLTNFLQLAEDNSKTELLIASIEQKAKSLQPIPFSNAINFKKNTKYLPWAIIP
                     LLFLFFFILTGNSDIIAESMNRVVRYNEKFLPPAPFSFVVLNDNLQTEQGQDFILQVK
                     SEGKIVPENAMIFIGDESYFMESTKPGVFEYIFSKPSKNVAFHIEANAVSSSDYELSV
                     VAVPTISNFEMTLQFPGYLNRKTEIIKGSGNAIVPEGTKVSWKVEAFATNQIQFSDGQ
                     LLTTFSKEENLFKYNKSINQNTEYQILTSNSKVKHYEKLNYQITTVRDQYPTITVNNA
                     PDSLHVDKNVIIGQVSDDIGLHKLQIVYYPQNNDKAALKSVLPV"
BASE COUNT          962 a          508 c          449 g         1039 t
ORIGIN      
        1 tcaatcgctt gattgtttcc tcttttccaa tcaattcaat aatatcaaac aaatgagggc
       61 cttttaaagc tccaaccatg ctcaatcgaa gcggttgcat gacttttccc atgccaattt
      121 cgttgactgt catccaatct tttagaatgg tttcaatgtt taaggatgta aaatcttcta
      181 ttgattctaa aacttctatc acttgtttca ttaatgttgg cgtttcttct ttccaatttt
      241 ttgatgcttt ttcatcatac gaagtcggtt caacaaagaa ataatcggct aaatcccaga
      301 aatcagaaac aaaattcgct cgttctttta tagaagaaac aattttgata agtttatagt
      361 tttcaacaga aattcctttt tcttttaaaa tagaagaaaa tatgtctgcc aattcttctt
      421 cattttgttt ctgcaaatac tgatgattga accatttatt cttctcaggg tcaaatttag
      481 ctccggcttt gtgcacacga ttcaaatcga atttttctgc caattcttct aaagtaaata
      541 attcttggtc ggttccatca ttccatccta ataacgctaa aaaattaatg acagcttccg
      601 gaaaataacc tttttcacga taaccggaag aaattccttc ttccgttttc cattccaaag
      661 gaaaaaccgg aaagcccatt ttatcaccat ctcttttaga taattttcca tttccaatgg
      721 gttttaaaat gagtggtaaa tgtgcaaatt caggtgcttc ccaaccaaaa gctttgtaca
      781 acaaatgatg caatggaagc gaaggcaacc attcttcgcc acgaatcaca tgcgaagttt
      841 ccatcaaatg atcatctaca atgttggcta aatgataggt tggcattcca tcgcttttaa
      901 ataatacttt gtcatctaac aggttggtgt caaattttat atcaccacga acaatatctt
      961 ttaattctaa aatttcattt actggcgttt taaaacggat gacaaaatct tcaccggcct
     1021 ctattcttct gtttgtttct tctgttgaca tcaccaacga ggtatctaat ttttcgcgat
     1081 tgtgccaatt ataaataaat gtttttcctt gttcttcgtg ctgttttctt tgtaaatcca
     1141 aggcttcagc cgtatcaaaa gcataatatg cccaaccgga attaatcaat tggtcagcat
     1201 attgcttgta taaatgtttg cgttcgcttt gacgataagg accaaacttt tcattttttc
     1261 caacggtttc tgacggtgaa attcccaacc attctaaagc ttcaaaaatg tattgttctg
     1321 caccgggtac aaacctgttt tgatcggtat cttcaatacg taaatagaat acaccgttat
     1381 gttttttagc aaataaatag ttaaataaag ctgttctaac accaccaata tgtaaaggtc
     1441 cggttggact aggggcgaaa cgaacacgaa caggttttga catgattttt caaattttga
     1501 gcaaagataa caagtttgaa tgagtgcgga agttatgagt agattttaat tcatttttgt
     1561 ctatttttaa gcgttttcat tagagaatat ttaacttttg actaaaaaat aaaattgtac
     1621 ttttatcggt tataaataaa gtccatttaa tgttggaaat aaaatcattg atatatcaaa
     1681 agttagaagt atttatcaaa aaatattata ctaacgaact tctcaaagga ttaattttct
     1741 ttgttggatt gggtttgata tatatgtttt ttacgctatt cattgaatat ttcctttggt
     1801 taaaaccaac cggtagaacc atcttgtttt ggttgtttat actagtggag ttatttcttt
     1861 tggctcgatt tatattgttt cctttattta aattaatcaa acttcaaaaa gggattgatt
     1921 atactcaagc ttccaaaatc attggcaatc attttacgga agttagtgat aaactgacta
     1981 attttttaca attagcagaa gataacagca aaacggaatt acttattgcc tcgattgaac
     2041 aaaaggcgaa atctttgcag cctattccgt ttagtaatgc tattaatttt aaaaagaaca
     2101 caaaatattt accttgggca attattcctt tgttgttttt gttctttttt atattaaccg
     2161 ggaatagcga tattattgcc gaaagcatga atagagtagt tcgttataat gaaaaatttt
     2221 taccgccggc accattttct tttgtagtat taaacgataa tttgcaaaca gaacaaggtc
     2281 aagattttat attacaagta aaatccgaag gtaaaattgt tcccgaaaac gcaatgattt
     2341 ttattggtga tgaaagttat tttatggaaa gcaccaagcc cggagttttt gaatatatct
     2401 tttctaaacc aagtaaaaat gtcgcttttc atattgaagc aaacgctgtt tcctcttcag
     2461 attatgaatt atcggtagtg gctgttccaa ctatttcaaa ttttgaaatg actttacaat
     2521 ttccaggcta cttaaatcga aaaacagaaa tcattaaagg ttccggtaat gctattgtgc
     2581 ccgaaggaac gaaagtgagc tggaaagtcg aagcttttgc tactaatcaa attcagtttt
     2641 ctgatggcca attacttact actttttcaa aagaggagaa tttatttaaa tataataaaa
     2701 gcataaatca aaatacagaa tatcaaatac ttacatcaaa ttctaaagta aagcattacg
     2761 aaaaattaaa ttatcaaatc acaaccgtta gagatcaata tccaactatt accgtaaata
     2821 atgctcctga tagtttgcac gtagacaaaa atgtcataat tggtcaggtt tctgatgata
     2881 ttggtttaca taaacttcag atcgtctatt atcctcaaaa caatgataaa gcagccttaa
     2941 aatctgttct tcccgtta
//
LOCUS       DAOTCW010000218         2949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_27797_length_2949_cov_38.206634, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000218 DAOTCW010000000
VERSION     DAOTCW010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27797_length_2949_cov_38.206634"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1391
                     /locus_tag="P5335_11545"
     CDS             174..1391
                     /locus_tag="P5335_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018945462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRZ75559.1"
                     /translation="MASIKLILRKHQLDTTGHCPLYIRIIKDRKTKFITAGVKLKESE
                     WDEVKQKVKKNHPNSARMNASLAQKIADAEAQVADLERKVKTVSIKKLKEAIKGKEVP
                     NFFDYAYKRMEIIRSTVSYSTYKAYYAAIVKFEKYMGTRDVYFDDFSVTLLNDYKNHL
                     SINLKNGLTTQRLSIIVLGTIFNQAIKEDVIPETMFPFKKLTLKIDNAKRLFLNKDKI
                     VGLVDLELPEGKKAAMWRDLFLFSIYAGGLRFSDVIEMQYKHYNKEEHKISKLIRKTG
                     RTHQFKIGKVAIEILEKYMKEGANNEDYIFPVIEDITGYEKNDTMRYFLTAKYNKLAN
                     WHLKKMGEKMKLSFPLSFHLSRHTFATNALNNGMRIEHVSKLMDHRDISTTQVYAKII
                     GEELDKAVDNYIY"
     gene            1641..2549
                     /locus_tag="P5335_11550"
     CDS             1641..2549
                     /locus_tag="P5335_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75560.1"
                     /translation="MNSQVENIKTAPFPEHVAVPTAQAQNPLFDIIIEEEFTLQKYCN
                     ALIEKILTLRQSQFPSFINYQCNQVKNPLTWINKLEKLLANNETFFSSKTALSRYNKL
                     YTLIDRKRIELQSTSVKEPISKTPKKYINAESEDRYFSFYEIKKQLEKLENDNEKILL
                     LTKEKFEYQQANIEFINQKTPLYDEQCCKEIEHIYALQKLQAALELNKKESNISQIPF
                     NKVKFNCNVNQFVDIFYQLHRELFCEGKPFIDGSMNDLVAIIVNSFVDKDGNEISPQT
                     VETILKPSRTDKRPKPHKRIDIDKML"
     gene            2639..2917
                     /locus_tag="P5335_11555"
     CDS             2639..2917
                     /locus_tag="P5335_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRZ75561.1"
                     /translation="MEAIILTKEQYTEIVTRLENITQQLNSKVGPKKETFLDNQEFLL
                     LLKISKRTAQTWRDEGKISFSQVGNKIYYKLSDVEKLMQENYNKAFKR"
BASE COUNT         1136 a          556 c          510 g          747 t
ORIGIN      
        1 ttttaggaag ttcataataa agcaataaaa agcagtcaaa tacttgtttt gttgtaccta
       61 atgttgtacc ttagtaccat gttgcaccag tttgaaacac cagtattttc agtactttca
      121 ccctattgca tcactttaca aatatagtac aacaatccta atttgcaaat tttatggcat
      181 ctatcaaact tatactccga aaacaccaat tagacaccac cggccactgt ccgctttata
      241 tccgaattat aaaagacaga aaaacgaaat ttatcactgc gggcgtcaag ctcaaagaga
      301 gcgaatggga tgaagtaaag cagaaagtaa aaaagaacca tccaaacagt gcccggatga
      361 atgcatcgtt ggctcagaaa attgctgatg ccgaagcaca ggtggcagat ttagaaagaa
      421 aggtaaaaac ggtttccatc aaaaagctca aagaggctat caagggaaaa gaagtgccga
      481 actttttcga ctatgcctac aaacggatgg agatcattag aagtacagta tcctattcca
      541 catacaaagc ctactatgca gcaatagtaa agtttgaaaa gtacatggga accagagacg
      601 tttactttga tgacttctcc gtaacgcttt tgaacgacta caaaaaccac ctaagcatta
      661 atttgaaaaa cggactaaca acacaacggc tttcgattat tgtgttggga accatcttca
      721 accaggcgat aaaagaagac gttattccag aaaccatgtt tccattcaaa aagctgacac
      781 tcaaaatcga taacgctaag cgattatttc taaataaaga caagattgta ggtttagttg
      841 atttggaact ccccgaaggc aagaaagcag caatgtggag agatttattc ctattctcca
      901 tctatgccgg tgggttgcgt ttcagtgatg tgattgaaat gcaatacaaa cactataaca
      961 aagaagagca taaaataagt aaactaatca gaaagaccgg aagaacacac cagttcaaaa
     1021 ttggaaaagt ggccatcgag atcttggaga aatacatgaa agaaggtgcc aataatgagg
     1081 attacatctt tcctgtgatt gaagatataa ccggctatga aaaaaatgat acgatgcgtt
     1141 attttcttac cgcaaaatat aataaattag cgaactggca tttgaaaaaa atgggagaaa
     1201 aaatgaaact ttcatttcca ttatcctttc atttgagtcg ccatactttt gcaacgaacg
     1261 cgttaaataa tggaatgaga attgagcacg tttcaaaatt aatggaccac cgagacatca
     1321 gtacgacgca ggtttatgca aaaattattg gtgaagaatt agacaaagca gttgacaact
     1381 atatttatta agagtaccgc caagcaccca ttttattgac aatcgcagat tgttaataac
     1441 ttcaattaaa atcctttaac aaggattttt tttattttct aattttacga aatagaaaac
     1501 aagtaatttt cggagcaaac aaatcgaaaa aaagttgaat gctattcgcc aaatcaaata
     1561 ccggaataga acgacatatt tcaagcgact ttaaattaca atttcctaaa aaatgaacat
     1621 aaaaaaagta aatactacaa ttgaattcac aggtagaaaa tataaaaacg gctccgtttc
     1681 cggagcatgt agcggtgccg acagcacagg ctcaaaaccc cctatttgat attattatcg
     1741 aagaagaatt cactttgcaa aaatattgca atgctttaat tgaaaagata ctcacacttc
     1801 gccagtcgca atttccttca ttcataaatt accaatgcaa ccaggttaaa aatccgttga
     1861 cctggattaa caagctggaa aagttattag ccaacaacga aacctttttt agttccaaaa
     1921 cagccctaag cagatacaat aagttataca ccttaatcga tagaaaacga attgagctgc
     1981 aatccacgag cgtaaaagaa ccgatcagta aaacaccgaa aaagtatatc aatgccgaga
     2041 gtgaagaccg gtatttctcg ttttacgaaa tcaaaaaaca gttggaaaaa ttggaaaatg
     2101 acaacgaaaa aatactgttg ttgaccaaag aaaaattcga gtaccaacaa gccaatatcg
     2161 aattcatcaa ccaaaaaaca ccgttgtatg atgagcaatg ctgcaaagag attgagcata
     2221 tatatgcctt gcaaaaactg caagcagcgt tggaactcaa caagaaagaa agcaacatca
     2281 gccaaatacc attcaataaa gtaaaattca attgcaatgt gaatcagttt gtggatattt
     2341 tctaccaact acaccgggag ttattctgcg aggggaaacc atttattgat ggttccatga
     2401 atgatctggt ggcaatcatt gtcaattcat ttgtcgataa agatgggaat gaaataagtc
     2461 cccaaacagt cgaaacaata ctcaaacctt cgcgtaccga caaacgtccg aaacctcaca
     2521 aaagaataga catcgacaaa atgctctaat acaaaatagt ccctcgggac tattttggga
     2581 cttttggact ttttttctcg tccaacattt gcaccataac ttttaaatca tttaaaaaat
     2641 ggaagcaatt attttaacga aagagcagta cactgaaatc gtaacgcgtt tagagaacat
     2701 cacccaacaa ctcaactcaa aagtaggtcc aaaaaaggaa acctttttag acaatcaaga
     2761 attcctctta ttactcaaaa tctccaaaag aacagctcaa acctggagag acgaaggtaa
     2821 aatttcattc agtcaagtcg gaaacaaaat ctactacaaa ctatcggatg tagagaaatt
     2881 gatgcaagaa aactataaca aagctttcaa aagataatcg tatgaaaagc tgcaacggaa
     2941 cctgtgaag
//
LOCUS       DAOTCW010000219         2933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_27994_length_2933_cov_31.882210, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000219 DAOTCW010000000
VERSION     DAOTCW010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27994_length_2933_cov_31.882210"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="P5335_11560"
     CDS             complement(<1..901)
                     /locus_tag="P5335_11560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRZ75562.1"
                     /translation="MRLHRNLVYTTIDSLNAIFNEGEYADKVVARALKKDKRWGSSDR
                     KFVAETIYEIVRWKRLYGEIAEVKEPYDRDDLWRMFAAWAVLRGYPIPDWRQLEGTPQ
                     RKIKGRFDELSKIRKYRESIPDWMDEIGVKELGEELWSKEIAAQNQQAQVILRVNTLK
                     TTKQKLRAILMDLNIETDYLEKQADALVLKERANVFLTDAFKEGLFEVQDANSQLVAA
                     FLDVKPGMKVVDTCAGAGGKTLHMASLMENKGLLIAMDLYESKLKQLKLRAKRNGAFN
                     IEYRIIDSTKVIKKLYEKADRVLI"
     gene            complement(1028..2647)
                     /gene="pckA"
                     /locus_tag="P5335_11565"
     CDS             complement(1028..2647)
                     /gene="pckA"
                     /locus_tag="P5335_11565"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRZ75563.1"
                     /translation="MKNIKVIQELHDLGITGYHEVVYNPSYEELFQAETSHMRKGYEK
                     GALTDTGAVAVKTGVFTGRSPKDRYIVKDEITENTICWDGKVNFPTTNEIWKDLKSLV
                     LKQLSISKKLYVVDAFCGTNPDTRLKVRFVMEVAWQAHFVTNMFIRPSIYELENFGEP
                     DFIVMNGSKTTNPNWAAQGLNSENFVVFNLTEKIQIIGGTWYGGEMKKGMFAMMNYYL
                     PLKGMASMHCSANVGEKGDVAVFFGLSGTGKTTLSADPKRYLIGDDEHGWDNNGVFNY
                     EGGCYAKVIDLSEENEPDIWRAIKRDALLENVVVNEYGEVDYYDHTITENSRVSYPIY
                     HINKIVLPSKAGHAKKIIYLSADAFGVLPPVSILDEDQAQYHFLCGYTSKLAGTERGI
                     TAPEPSFSPAFGEAFLTLHPTMYSKTLIGKMKEHGAKAYLVNTGWNGTGKRISLKNTR
                     AIIDAIINGEIDKMETRHIPFLNLTVPTSLPNVDKGILDPRDTYKSEEEWEVKAKDLA
                     ARYIKNFEQYTDTEEGKKLVYAGPSLENVLA"
BASE COUNT          888 a          548 c          442 g         1055 t
ORIGIN      
        1 cgattaatac acgatcagct ttttcgtata gttttttaat tactttcgta gaatcgataa
       61 tacggtattc aatattgaaa gccccatttc gttttgctcg aagttttagc tgttttaatt
      121 tactttcgta taagtccatc gcaattaata accctttgtt ttccattaag gatgccatat
      181 gcaaggtttt tccaccagca ccagcacaag tgtcaactac tttcattccg ggttttacat
      241 ctaaaaaggc agctacaagt tgtgaatttg catcttgtac ttcaaataaa ccctctttaa
      301 aagcatcggt taaaaaaaca tttgcccgct ctttcaagac taaggcatct gcttgttttt
      361 caaggtaatc tgtttcgata tttaaatcca tcaagattgc ccgcaatttt tgttttgttg
      421 tttttaaggt attcactctt aaaataactt gagcctgttg gttttgagca gcaatttctt
      481 tcgaccaaag ttcttctcct aattctttta caccaatttc atccatccaa tcaggaatag
      541 attcacggta ttttcttatt ttggataatt catcaaaacg acctttgatt ttgcgttgcg
      601 gagttccttc caattgtctc caatcaggta ttgggtagcc tcttaatacg gcccaagcgg
      661 cgaacattct ccataaatca tcccgatcgt aaggttcttt tacttcagca atttcgccat
      721 ataaacgttt ccatcgaaca atttcataaa tggtttcagc cacaaatttt ctatcggaac
      781 ttccccaacg tttgtctttt tttaatgctc tagcgacaac tttatcagca tattcacctt
      841 cgttaaatat ggcattcaag gaatcaattg ttgtgtaaac taaatttctg tgtaatctca
      901 tttcaataaa atagcctgca aaggtactat taattatgag tcagttaact aataaacgtt
      961 aaaaattcaa aacccgacgg aaaaatccaa tcgggttttg agaaaaacaa gtgtcgttgc
     1021 gtttgagtta tgctaataca ttttctaagc ttggaccggc ataaaccaat tttttacctt
     1081 cttctgtatc agtatattgt tcaaagtttt tgatataccg agcagctaaa tctttggctt
     1141 ttacttccca ttcttcttct gatttataag tgtctcttgg atctaaaata cctttatcaa
     1201 cattaggtag agaagttgga accgttaaat tcaagaaagg aatatgtctt gtttccattt
     1261 tatcaatttc tccattgatt attgcatcga tgatggcacg agtgtttttc aaagaaattc
     1321 tttttccggt accattccat ccggtattta ctaaatatgc ttttgctccg tgttctttca
     1381 tttttccaat taatgtttta gagtacatgg tcggatgtaa agttaaaaaa gcttcaccga
     1441 atgcaggtga gaatgaaggt tcaggtgcag taattcctct ttctgttcct gccaatttgg
     1501 aggtataacc acataagaaa tggtattgag cttggtcttc atcaagaata gaaactggag
     1561 gcaatacacc aaaggcatca gcagaaaggt aaattatttt ttttgcatga cctgcttttg
     1621 atggtaatac aattttattg atgtggtaaa ttgggtaaga aactctagag ttttcagtaa
     1681 tagtatggtc ataataatct acttcaccat attcatttac cactacattt tctaataagg
     1741 catcccgttt gattgctctc caaatatccg gttcgttttc ttcactaagg tcaattactt
     1801 tagcgtagca accgccttcg taattaaata ctccgttgtt atcccagccg tgttcatcat
     1861 ctccaattaa ataacgtttt gggtcggcag ataaagttgt ttttcctgtt cctgataatc
     1921 cgaagaatac cgctacatct cctttctctc ctacattagc agaacaatgc attgatgcca
     1981 ttccttttaa aggaaggtag taattcatca tggcaaacat tccttttttc atttcaccgc
     2041 cataccaagt accaccaata atttgaattt tttcagttaa attgaaaact acaaagtttt
     2101 cagaatttaa gccttgtgct gcccaatttg gattggttgt tttagaacca ttcatcacaa
     2161 taaaatcagg ttcaccaaaa ttttctagtt cataaatgga aggacgaata aacatattgg
     2221 ttacaaaatg agcctgccaa gctacttcca ttacaaaacg tacttttaaa cgagtgtctg
     2281 gattggttcc gcaaaatgca tccaccacat ataatttttt tgagatagaa agttgtttta
     2341 aaactaatga ttttaaatct ttccaaattt catttgtagt tggaaagtta actttcccat
     2401 cccaacaaat ggtattttca gtaatttcat ctttaacaat atatctgtct ttaggagaac
     2461 ggcctgtaaa aactccagtt tttactgcaa cagcaccagt atctgttaaa gctccttttt
     2521 catatccttt tctcatgtga gaagtttcgg cttgaaataa ttcttcataa gaaggattgt
     2581 aaacaacttc gtggtaacct gtgattccca aatcgtgaag ctcttgaatg actttgatat
     2641 ttttcatggt tctaaaattt atttatttaa cgtctatttt ttatttgatt ataacttgaa
     2701 atcttatact ccaaaggtca ttaaatttac ttccaataaa gatgataaat gtcatgaaaa
     2761 tcagaaatct acgaaaacga tttcattact ttgtgttgat ttataagtga ttacgaatga
     2821 gtaacttttc aattttataa tttgaaaaag attactaaag ctatacttaa aagtaccaaa
     2881 aaacattcgt aattttgcag tcgtttaact gatatttaac tactaaataa aaa
//
LOCUS       DAOTCW010000220         2905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_28386_length_2905_cov_70.487368, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000220 DAOTCW010000000
VERSION     DAOTCW010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28386_length_2905_cov_70.487368"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="P5335_11570"
     CDS             complement(<1..395)
                     /locus_tag="P5335_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75564.1"
                     /translation="MKKIILLLAFFLSITTFAQKTVYIFNFSSYNVQVGEIQTRSTAT
                     SSYPRFRTNYSGGIINVPSGTTYTLENPNVNYFPFYSPTSVPYINAWQRQFTAGATWA
                     NFLSTNLNNALALPQRFNFIKFQVGPNGSL"
     gene            complement(404..778)
                     /locus_tag="P5335_11575"
     CDS             complement(404..778)
                     /locus_tag="P5335_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75565.1"
                     /translation="MKNFKNYLLLIFAVFFVACASEEEITSTTSKMPEAAKTPEMTNF
                     RNSMIEWLKIKNSPSFKINVNAEKEEKTKNDILLENAELLLIANGLESAVEASKKRED
                     NASIITMALQLYAEKTKIVTSN"
     gene            1169..1366
                     /locus_tag="P5335_11580"
     CDS             1169..1366
                     /locus_tag="P5335_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRZ75566.1"
                     /translation="MSSKTLQKIRQLRNDKNLTQEFVAEQIGCSQSHYAKLENGKVKL
                     SIEILQKISTVFKLSVSELLE"
     gene            1496..2545
                     /locus_tag="P5335_11585"
     CDS             1496..2545
                     /locus_tag="P5335_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75567.1"
                     /translation="MSEEKPQLKKQYYPINSNLRSYLEKYKRITKTPIYYDDLLRFQG
                     SIAVLDKDEKDTLWIRLYYNEFEKIELDTSLKKIYTLLHSDGDESNMEYLNVDYIDFC
                     TFGNSKPFRVKIRNILNDNYTHFYIKKADASRIFGLELEHIISPNSINFLVFEDTLIE
                     EHIVGIPGDVFLEQNLKECNEHEKTQIAKEFVKFNERCMIGLLGDMRSYNFVVIPIYD
                     FDQRIFRIRAIDFDQQSYEGSFKLYKPHLFKENFKFSELVQDKLNPKSIIQYQIEERS
                     IIVKRLIDTKTRLKRLIEAMKATKLAPIEHIQQLQEEIYDVTFDVEFKKANSMGEIVQ
                     AALNFMKRNYKIEDI"
     gene            2571..>2905
                     /locus_tag="P5335_11590"
     CDS             2571..>2905
                     /locus_tag="P5335_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HRZ75568.1"
                     /translation="MNQIQFIKSAVQTADKNIEATLKLLSEDCTIPFISRYRKDQTGN
                     LDEVEIENIAKLAKQYDEIVKRKDSILKTIEEQGQLSPELKSKIQNSFDLQEMEDLYL
                     PYKKKKKTKA"
BASE COUNT         1080 a          410 c          458 g          957 t
ORIGIN      
        1 agcgagccat taggaccaac ttgaaactta ataaaattaa atctttgtgg taaagccaaa
       61 gcattattta agttagtact tagaaaatta gcccaagtag caccggcagt aaattgtctt
      121 tgccaagcat taatataagg cacacttgtg ggagaataaa acggaaaata atttacattt
      181 gggttttcta atgtataggt agtacctgac ggaacattga taattcctcc tgaataatta
      241 gttctaaatc taggataaga ggaagtagcg gttgatcttg tttgaatttc tccaacctga
      301 acgttatagc tggaaaaatt aaaaatatat actgtttttt gagcaaaagt agtgatgctt
      361 aaaaagaagg ctaaaagtaa aatgattttt ttcatgtctt tgtttagtta gaggttacaa
      421 ttttggtttt ttcagcataa agttgcaatg ccattgtaat gatagaggca ttatcttctc
      481 ttttttttga cgcttcaaca gctgattcta atccatttgc tattagaagt agctctgcat
      541 tctctaataa gatgtcattt ttggtttttt cttctttttc agcattaaca tttattttaa
      601 aactgggtga gtttttaatt tttaaccatt caatcattga gttcctgaaa tttgtcattt
      661 caggggtttt tgccgcctct ggcattttac tcgttgttga tgttatttct tcttctgagg
      721 cacaagcaac aaaaaaaacg gcaaaaatca gtaggaggta atttttaaaa tttttcataa
      781 atttgcatta attaaatgaa ttgcagtctt actatgtcgt cgaaaacata taaataagta
      841 aggctgcttt tttgtgtttg tttggtcatt caaattgttg ctcaacaaac ttcaatagtc
      901 aacaattagt ttctaaaaag tacttacaaa aaactagaaa agcatttttt gtattgtgtt
      961 acaagtgggt ggttagtcct tttaaacctt atacgtttga ttattaactt gataattcca
     1021 aatataagga gttaaaaaca ataaacctag tcagaatctg actaaaatta gcacttaaat
     1081 tttgtaggaa tcattttttt tccccctact tttgtaaaac ccaaaacaaa ttagcctaaa
     1141 acagttattc atgttttaat gcaatgctat gtcttctaaa accttacaaa aaattcgtca
     1201 actcagaaac gataaaaacc ttacacaaga atttgtggca gaacagattg gttgttccca
     1261 aagtcattat gccaaactgg aaaatggaaa agttaaacta agcatagaaa ttctacaaaa
     1321 aatatctacg gtatttaagt taagtgtttc ggaattgcta gagtaacatt atcatgagtt
     1381 gtaatgtttc tattcaaccc gaatttcaaa aagctgatcc gaaagatttt attgtgcagg
     1441 attagttttt attttcaatt aaaaggagta ctttacagca gcagtaaatt aaaaaatgag
     1501 cgaagaaaaa ccacaattaa aaaaacagta ttatccgatt aattccaatt taagaagtta
     1561 tcttgaaaaa tataagagaa taacaaaaac acctatttat tatgacgatt tgcttcgttt
     1621 tcaaggaagc attgctgttt tagataaaga cgaaaaagac acattatgga ttcgtcttta
     1681 ttataacgaa tttgaaaaaa ttgaactaga taccagttta aaaaaaatct atactttatt
     1741 gcattcagat ggcgacgaga gcaacatgga gtatttaaat gtagattata tagatttttg
     1801 tacgtttgga aattcaaaac cctttcgggt aaaaattaga aatattctaa acgataatta
     1861 cacgcatttt tatatcaaaa aagcggatgc ttctcgaatt tttggtttag aattagaaca
     1921 cattatttcg ccaaatagta ttaattttct agtttttgaa gacacactta ttgaagaaca
     1981 tattgtggga attcctggcg atgttttttt agaacaaaat ttaaaagaat gtaacgaaca
     2041 cgaaaaaaca caaatagcta aagagtttgt aaaatttaat gaacgttgca tgattggttt
     2101 attgggcgat atgcgttcct ataattttgt ggtgattcct atttatgatt ttgaccaacg
     2161 tatttttaga atccgagcta ttgactttga tcaacaaagt tatgaaggaa gttttaaact
     2221 ctataaaccg catttattta aagaaaactt taagttttca gaactagtac aagacaaatt
     2281 gaatcctaaa tcaataattc aatatcaaat tgaagaacgc tccattattg taaaacgttt
     2341 aattgataca aaaactagat taaaacgatt aattgaagca atgaaagcaa caaaattagc
     2401 cccgattgaa catattcaac aacttcaaga ggaaatttat gacgttacgt ttgatgttga
     2461 gttcaagaaa gctaattcca tgggagaaat tgtgcaagcg gctttaaatt tcatgaaaag
     2521 aaattataaa attgaggata tttaatattt agtacagatt tatttttaca atgaaccaaa
     2581 tccaattcat aaaatcagca gttcaaacgg ctgataaaaa catagaagcc acgttaaaat
     2641 tactttcaga agattgtacc attcctttta tttcgcgtta ccgaaaagac caaaccggaa
     2701 atttggatga agtcgaaatc gaaaatatag ccaaattggc caagcaatat gatgaaatcg
     2761 taaaacgaaa agattccatt ttaaaaacta ttgaagaaca aggacaacta tcaccagaat
     2821 taaaatcaaa aattcaaaat agtttcgatt tacaagaaat ggaagatttg tatttgccat
     2881 acaaaaagaa gaaaaaaacc aaagc
//
LOCUS       DAOTCW010000221         2890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_28615_length_2890_cov_38.269841, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000221 DAOTCW010000000
VERSION     DAOTCW010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28615_length_2890_cov_38.269841"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /gene="recF"
                     /locus_tag="P5335_11595"
     CDS             <1..1040
                     /gene="recF"
                     /locus_tag="P5335_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964102.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRZ75569.1"
                     /translation="SETQFEFDTKINCFVGKNGVGKTNVLDAIYHLAYGKSYFNPLAV
                     QNIKHGEEFFVIEGDFDKNNRNEQIVCSLKKGLKKILKRNGKVYDKFSEHLGLIPLVI
                     ISPTDSDLITEGSETRRKFMDSVISQLDLSYLNQLIQYQKIIVQRNALLKYFALNQTF
                     DKDTLQIYNEQLQALGQTIFDKRKEFIENFLPIFNKHHHAITDSAELVNLVYESHLFE
                     NNLEKLLEINLAKDKALQYTSVGIHKDDLSFEIDGYPIKKFGSQGQQKSFLIALKLAQ
                     FEFLKKNSGQKPILLFDDIFDKLDEFRVSKIVEMVNHDEFGQLFISDTHPERTENIVK
                     STHQSYKIFKL"
     gene            1045..1719
                     /locus_tag="P5335_11600"
     CDS             1045..1719
                     /locus_tag="P5335_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HRZ75570.1"
                     /translation="MIQASALQFIDDLKNNNNKEWFHENKKRYDLFKKEYQTLIAALL
                     QEMKPLDASLELLEVKHCTFRINRDIRFSKDKSPYKSNMGIWMTTDRNRKNAPGYYIH
                     YEKGSSFIAAGIHCPEPEDLKKIRKEIAFFQEDLEEILANPSFKKEYGSLDRDESNVL
                     KKAPKDYDPNHPAIEFLKLKSFTATQKIDDSLFTDVDFAKKMAQKLIILKPLTVFLNR
                     ALETIE"
     gene            1729..2742
                     /gene="murB"
                     /locus_tag="P5335_11605"
     CDS             1729..2742
                     /gene="murB"
                     /locus_tag="P5335_11605"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRZ75571.1"
                     /translation="MQIHKNFSLKKYNTFGIEAKAGQFVSVQSVDELITVLNENPTTK
                     KFILGGGSNMLLTQDINALVIHLDLKGKKIIDENDDFVWVESQAGENWHEFVIWTIDQ
                     NFGGLENLSLIPGNVGTTPIQNIGAYGVEIKDTFVLCEAIHIETQELMIFTNEKCKFG
                     YRESIFKNEVKDEFIITSVVFKLTKKNHKLNTSYGAIETELEKQHITNPTLKDVSNAV
                     ISIRQSKLPDPKELGNSGSFFKNPVILKTDFEKIQQKFPEMPHYVVSETEVKVPAGWL
                     IEQAGFKGKRFGDAGIHKNQALVLVNYGKATGQEILAVAKDIQATIEATFSISIETEV
                     NII"
BASE COUNT         1097 a          420 c          469 g          904 t
ORIGIN      
        1 tttctgaaac tcaatttgag tttgacacca aaataaattg ttttgttgga aaaaatggag
       61 taggaaaaac taacgttctc gatgcaattt atcatttagc ctatggcaaa agctatttta
      121 atccgttggc tgtacaaaat ataaagcatg gagaagaatt ttttgttatt gaaggtgatt
      181 ttgacaaaaa caatcgcaac gaacaaattg tctgtagttt aaaaaaaggg ctcaaaaaaa
      241 tcttaaaacg caatggaaaa gtttatgata aattctctga acatttagga ttaattccct
      301 tagtcatcat ttcgcctact gatagcgatt taataacaga gggtagtgaa acccgacgga
      361 aattcatgga cagcgtgatt tcacaactag atttatccta tttaaatcaa ctcatccaat
      421 accaaaaaat cattgtacaa cgcaatgcct tattgaaata ttttgcttta aatcagacat
      481 ttgataaaga tactttgcag atttacaatg aacaattaca agctttaggg caaactattt
      541 ttgataaaag aaaagagttt atagaaaatt ttctacctat atttaataag catcatcatg
      601 ctataactga ttctgctgaa ctagtcaact tggtctatga aagccatctg tttgaaaata
      661 atttggaaaa actattagaa attaacttag ccaaagataa agctctgcaa tatacttctg
      721 ttggtattca caaagatgat ttgtcgtttg aaattgatgg ctatccaatt aaaaaatttg
      781 gctcacaagg acaacaaaaa tcttttttaa ttgctttaaa actagctcaa tttgaatttt
      841 taaagaaaaa tagtggtcaa aaacctatat tgctatttga tgatattttt gataaattag
      901 acgaatttag agtaagtaaa attgtcgaaa tggttaacca cgatgaattt ggtcaacttt
      961 tcatttctga tactcatcct gaacgaacag aaaatattgt aaaatcgaca catcagagtt
     1021 ataaaatttt taaattataa aatcatgatt caagctagtg cactccaatt tattgacgat
     1081 ttaaaaaaca acaataacaa agaatggttt catgaaaaca aaaaacggta tgatcttttt
     1141 aaaaaagaat accaaacttt gattgctgct ttattacaag aaatgaaacc cttagatgct
     1201 agtctggaat tactggaagt gaaacattgt actttcagaa tcaatcgtga cattcgcttt
     1261 tcaaaagaca aatcacccta taaaagtaat atgggaattt ggatgacaac cgataggaat
     1321 cgaaaaaatg cacccggata ttatatccat tatgaaaaag gaagcagttt tattgccgcc
     1381 ggaattcatt gtccggaacc ggaagatttg aaaaaaattc gtaaagaaat tgcttttttt
     1441 caggaggatt tagaagaaat tttagctaat ccaagcttta aaaaagaata tggaagttta
     1501 gacagagacg aatctaatgt gcttaaaaaa gctccaaaag attatgaccc taatcatcca
     1561 gccattgaat ttttaaaatt aaaaagtttc actgccactc aaaaaatcga tgattcgctc
     1621 tttacggatg tcgattttgc aaaaaaaatg gctcaaaaac ttattatttt aaaacctttg
     1681 accgtatttt tgaaccgtgc tttagaaact attgaataag agaataaaat gcaaattcat
     1741 aaaaattttt cattaaaaaa gtataacacc tttggtattg aagccaaagc cggacaattc
     1801 gtttcagttc aatctgttga tgaattgatt acagtcttaa atgaaaatcc aacaactaaa
     1861 aagtttatac ttggaggcgg cagtaatatg ttgctaacac aagacattaa tgctttagtc
     1921 attcatttag acttaaaagg aaaaaaaatc atagatgaaa atgatgattt tgtttgggtc
     1981 gaaagtcaag ccggtgaaaa ttggcatgag tttgtaattt ggacaattga tcaaaacttt
     2041 ggaggtttag aaaacttatc attaattccg ggtaatgtag gcactactcc cattcaaaat
     2101 atcggagcct atggtgttga aatcaaagat acctttgtat tgtgtgaagc tattcatatt
     2161 gaaacgcaag agttgatgat ttttaccaat gaaaaatgta aatttgggta tcgtgaaagc
     2221 atttttaaaa acgaagtgaa agatgaattt attataactt cagtagtttt taaattaacc
     2281 aaaaaaaatc ataaactcaa tacttcttac ggggcgattg aaactgaatt agaaaaacaa
     2341 cacattacta atccaacttt aaaagacgtt agtaatgcag tcatttctat ccgtcaaagt
     2401 aaattaccgg acccgaaaga gttaggaaat agtggaagtt tctttaaaaa tccggttatt
     2461 ttgaaaactg attttgaaaa aattcaacaa aaatttcccg aaatgcctca ttatgtagtt
     2521 tcagaaacag aagtgaaagt tccggccgga tggcttatag aacaagccgg atttaaagga
     2581 aaacgttttg gtgatgcagg aatacataaa aatcaagcct tagttttagt aaattacgga
     2641 aaagcaaccg gtcaagaaat attagctgta gccaaagata ttcaagcaac aattgaagct
     2701 actttttcta tttccattga gactgaagtc aatattattt gatgtttgga tagggtttat
     2761 ttcttaaatt aactttaaat atcagactaa taaagaacac ttttttgtat ctttaccacg
     2821 taaaaaaaac acttaaaaaa tgaaattggt cttaatcaca attggtcttc ttgcacttgc
     2881 ttttgctggg
//
LOCUS       DAOTCW010000222         2861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_29022_length_2861_cov_40.373485, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000222 DAOTCW010000000
VERSION     DAOTCW010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29022_length_2861_cov_40.373485"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1803
                     /locus_tag="P5335_11610"
     CDS             <1..1803
                     /locus_tag="P5335_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75572.1"
                     /translation="AYTVTGGGAICVGSAGIPIGLSNSQVGIIYQLIINGSNAGSPVA
                     GTGSAISFGNQNTIGTYTVEALNSACSLTLPMTGSVSISAGATTTWSGSPASWDNGAP
                     TSAMIAIISADYSEVADINACSLTVNNNAIVTIPSGNVISLFGGLTVESGSSFVLNSD
                     AVLLQDGTTNQNSGNITVNRSSQDLMRLDYTMWSSPVEGQVLAAFSPLTMANRFYTYS
                     SFDNFYTPIANTNSFTVGQGYLIRMPDNHPTSPTAWNGAFIGKPNSGTITIGLSTAGT
                     GFNAVGNPYPSPITISTFLSENSSVIGSDLYFWRKTNGAAGSAYITFSGGSFSDGPHT
                     NNTIQPGQGFIVQATNAADLTFTNIQRNANNGVFYRNGNVTQSEDNSRIWLNLKSNEI
                     IVCQMAVGYRAEATNSIDADFDAEYINDSDLALNSFVEGTEFAVQHRATFTASDVVPL
                     SFKTTTAGTFVIAINEVDGLFTNADQNIYLKDNLLNVEHDLRSAPYSFVSEIGVFANR
                     FEIVYQSALSVEVPTFGNGVVVYSKNKTIEINSGTEVMSSVKVLDIRGSIVAEKVAVN
                     STNTSIDLTYVANQVLIIQITAENGQTVSRKVVH"
     gene            2209..>2861
                     /locus_tag="P5335_11615"
     CDS             2209..>2861
                     /locus_tag="P5335_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75573.1"
                     /translation="MKYALFFVVFLLTENANSQTNKWDGSTDNNWNTAANWSLNLVPT
                     NAHDVVIDINAAIIVNTNATINKLTITSNSTVTFTSSGAGRTITIDNTGSSIDSGSSL
                     ELRGSTSFFGTRSMTIAYSGSNQTMSIAGTLNVTATGDGSVYIATNGLTTVTGTLISS
                     GGTITSTAANLTIASGGIYQHARNGGPIPTATWNAASTCLVTGITNTIPSAGLGQSFG
                     "
BASE COUNT          900 a          481 c          521 g          959 t
ORIGIN      
        1 gcctacactg taacaggtgg tggagccatt tgtgttggtt ctgctggaat tccaattggt
       61 ttgtctaact ctcaagtagg tataatttat caactaataa ttaatggttc taatgcaggt
      121 tcacccgttg caggaactgg ttctgccatt tcttttggaa atcaaaacac cataggaact
      181 tatactgttg aagctttaaa ctcagcatgt tctttgacat taccaatgac aggaagcgtt
      241 tcaatttctg ccggtgcaac aacaacttgg tcgggttctc ctgcatcatg ggacaatgga
      301 gctccaacaa gtgcgatgat tgccataatt tctgctgact attctgaagt ggctgatatc
      361 aatgcttgtt ctttaacagt gaataataac gcaatagtaa caatcccatc cggaaatgta
      421 atttcattat ttggtggatt aaccgttgaa tctggaagtt cttttgtttt gaatagtgat
      481 gcagtattat tacaagacgg tacaaccaat caaaactcag gaaatattac ggtgaatcgt
      541 agtagccaag atttaatgcg tttagattat accatgtggt cttcaccggt agaaggtcag
      601 gtattagctg ctttttcacc gttaacaatg gctaatcgtt tttataccta ttcatctttt
      661 gataattttt atacaccaat agcgaataca aatagtttta ctgttggaca aggttaccta
      721 atccgtatgc cggacaatca cccaaccagc ccaactgctt ggaatggtgc ttttattgga
      781 aaaccaaata gtggtactat taccataggt ttatctactg ccggaactgg ttttaacgca
      841 gtgggtaatc cttatccatc gccaattact atttcaactt tcctttctga aaacagtagt
      901 gttattggaa gtgatttata tttttggaga aaaacaaatg gtgctgccgg aagtgcttat
      961 ataacattta gtggcggatc gtttagtgat ggtcctcata caaataatac aatccaaccg
     1021 ggacaaggtt ttattgttca agctaccaat gcagctgatt taacgtttac caatatacaa
     1081 cgaaatgcta acaatggtgt tttctatcgt aatggaaatg taacccaatc agaagacaat
     1141 tcaagaattt ggttaaattt aaaaagtaat gagattatag tatgtcaaat ggctgttgga
     1201 tacagagccg aggcaacgaa tagtattgat gctgattttg atgcagaata tattaacgac
     1261 agtgacttag ctttaaattc atttgtggaa ggaactgaat ttgctgtgca acaccgtgct
     1321 acttttacag cttctgatgt tgtaccatta agttttaaaa caactacagc gggaacgttc
     1381 gtgattgcaa tcaacgaagt agatggtttg tttactaatg cggaccaaaa catttattta
     1441 aaagataatc tattgaatgt agagcatgat ttaagaagtg ctccttatag ttttgtgtct
     1501 gaaataggtg tttttgcaaa ccgttttgaa attgtttacc aatcggcttt atcagtggag
     1561 gttccaacct tcggaaatgg agtagtggtt tattcaaaaa ataaaaccat tgaaatcaat
     1621 tcaggaactg aagtgatgtc gtccgtcaaa gtacttgata tcagaggtag tattgtagct
     1681 gaaaaagttg ctgttaatag tacaaatacg tctattgatt tgacttacgt tgctaatcaa
     1741 gttttgatta tacagatcac tgctgaaaac ggtcaaacgg tttccagaaa agtagttcac
     1801 taaggcataa attatatagt actaataaac ccatcagtta tctgatgggt ttattatttt
     1861 tctaatattt tgagtgttag gtttttttat ttctttttct gatattattt catatatttg
     1921 ccacaattac cacattttct aaaaacaaac cccagattta cgtattagtt ttaatctcaa
     1981 aaattttata ttaaattatt tttgtaggtc aaaacttatt taaaatgtta attattacat
     2041 tttaacggct aaatatgtat ttaaacggct ttttgcttgt aaatcttatt taccctagct
     2101 atttttgctt ttaaaataac caatactatt ttattggaac aattgaattt aataacaaaa
     2161 ggtataacca tatacagcct tatcattatg aaaacaaaat tacacttttt gaaatacgct
     2221 ctgttttttg tagtatttct acttacagaa aatgccaatt ctcaaaccaa taagtgggat
     2281 ggttcaacag ataacaattg gaatactgcg gcaaactggt ctttaaatct ggtgccaact
     2341 aatgcacacg atgtagtaat tgatattaat gctgcaataa ttgtaaatac aaatgctaca
     2401 attaataaac taactattac aagtaattct acggtaactt tcacatctag tggagcaggt
     2461 cgtactataa caattgataa tacaggtagc tcaattgatt cgggttcatc tcttgaattg
     2521 agaggttcaa cgagtttttt tggaactagg tcgatgacaa ttgcttatag cggttcaaat
     2581 caaacaatgt caattgctgg aacgcttaat gtaactgcca ctggcgatgg ttcagtttat
     2641 atcgccacta atggtttaac aaccgttaca ggaactttaa ttagtagcgg aggaacaatt
     2701 acttctactg ctgcaaactt gactatagca tctggaggta tttatcaaca tgccagaaat
     2761 ggtggaccta ttcctaccgc tacatggaat gctgcctcaa catgtttagt gaccggtatt
     2821 accaacacta tccctagtgc aggtttaggt caatcctttg g
//
LOCUS       DAOTCW010000223         2723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_31285_length_2723_cov_38.080960, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000223 DAOTCW010000000
VERSION     DAOTCW010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31285_length_2723_cov_38.080960"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="P5335_11620"
     CDS             <1..276
                     /locus_tag="P5335_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRZ75574.1"
                     /translation="GFKLIRDTFIFTNKRLILIEKQGITGSKVEYKSILYKSISRFSV
                     ETAGTFDLEAELKIWVSSETHPSIVKQFNKGVDVYDVQKVLAHHVLG"
     gene            832..2493
                     /gene="glgP"
                     /locus_tag="P5335_11625"
     CDS             832..2493
                     /gene="glgP"
                     /locus_tag="P5335_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRZ75575.1"
                     /translation="MSSTLPKQYKHPYTFDKKYKTSVAYFSMEFAIDQSFKIYSGGLG
                     FLAGSHMRSAFDLKQNMIGIGVLWKQGYYDQIRKEDRSMGVMFQEKIFHFLEDTGIRF
                     TIEINNHPVWIKAMFLNPTTFGTAPMFFLSTDLPENDYLAQSTTFRLYDSDPNAKVAQ
                     CMVLGVGGAKLLDELNYQPDLYHFNEAHALSGAFHLYKKFGHNLDELKKRLVFTTHTP
                     EEAGNEKHDIYFLQNLSFFCGLPMEEVRAISGIYDNTFNHTLVGLRFSHKANGVSKLH
                     GEVSRHMWEGYDGVCPITHITNAQNKNFWADPFLEEARVANNPAGILKRKARLKHKLF
                     DVVADQTGKLFKDDVLTIVWARRFAEYKRPDLITRDHDRFRRLMDNKEFPIQLIFAGK
                     PYPMDYGAINTFNSLAYLSRSFDNMAVLTGYELKLSRQLKEGSDVWLNTPRVTREASG
                     TSGMSAAMNGSINLSTNDGWICEFYDNGVNSFVLPTVDHHLPTYEQDAKDLDNLLTLL
                     ENVVVPMYYKKRKDWNKIMIESMNTVVPFFDSARMVDEYYEKMYS"
     gene            2638..2722
                     /locus_tag="P5335_11630"
     tRNA            2638..2722
                     /locus_tag="P5335_11630"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2672..2674,aa:Ser,seq:tga)
BASE COUNT          846 a          442 c          548 g          887 t
ORIGIN      
        1 ggttttaaac tgattcgcga cacatttatc ttcaccaata aacgcttgat attgattgaa
       61 aagcaaggca ttaccggaag taaagtagaa tataaatcga tactttacaa aagcattagt
      121 cgcttcagtg tggaaactgc cggtactttt gaccttgaag cagaattgaa aatatgggtt
      181 tccagtgaaa cacatccaag tattgtaaaa caatttaata aaggagttga tgtgtatgat
      241 gtgcagaaag tgttggctca tcatgtgttg ggataggtat aaattatcat tcggagcttg
      301 ttaaacgtaa ccaaaacgat aacaaactcc tcctgacaac ctatattccg gggaaagtgt
      361 ggattgagta cacattaact tcctgaaccg gtttcattaa aatggtattg ttgtttgtta
      421 gtacctaatt ttacatcagg tgacgatgtt cttctcttga caagcaacaa tacaatttat
      481 ttttttacaa ctaattttag cttcattatt gtttagtaaa atacgggata aaagttgtca
      541 actcgtttat acaattctcg ctaaatgtta taaaattcat atttttattc atctgattta
      601 gccttttttt gttgaatagt cagcaaatga atcaaaagaa catttgaggc tgttttgacc
      661 tatattatca ctgaaattct atcttttata tagatattct tttttatatt tggcttggtt
      721 tttattgaaa ggcaatgctt tttgtatgct actgaaaaac agtgttctac tgatttattt
      781 cctttttaat tctaccgttt tctctatttt taatcgtata aaaaagacag catgagctca
      841 actttgccca aacagtacaa acatccgtat acctttgaca aaaaatataa aacatcggtg
      901 gcctatttca gtatggaatt tgccattgat caaagcttta aaatctattc cggaggattg
      961 ggttttttag caggttctca tatgcgaagt gcctttgatt tgaaacaaaa catgattggt
     1021 attggcgttt tatggaaaca aggttactat gatcaaattc gaaaagaaga tcgctcgatg
     1081 ggagtgatgt ttcaggaaaa gatttttcac tttttagaag ataccggcat tcggtttacg
     1141 attgaaatca ataaccaccc tgtttggatt aaagccatgt ttttgaaccc aaccactttt
     1201 ggcacggctc ccatgttttt tctttctacg gatttaccgg agaatgatta tttggctcaa
     1261 tcgactacgt ttcgtttgta tgactccgac ccaaatgcaa aagtggccca atgcatggtg
     1321 ttaggtgtag gcggagctaa gttgttagat gaattgaatt atcaacctga tttataccat
     1381 tttaatgaag ctcatgcatt atcaggagct tttcatttgt ataaaaagtt cggacataat
     1441 ttggatgaac ttaaaaaacg attagttttt acaacccata ctccggaaga agcaggaaat
     1501 gaaaagcatg atatttactt tttgcaaaac ctaagcttct tttgtggctt accgatggaa
     1561 gaggtgagag ctatttcagg tatttacgac aatacattta atcatacctt agtgggttta
     1621 cgtttcagtc acaaagcgaa tggcgtttcg aaattgcacg gggaagtatc cagacacatg
     1681 tgggaaggtt atgacggtgt ttgcccgatt acccacatca cgaatgcaca aaataaaaac
     1741 ttttgggcag atccattctt agaagaggcc agagttgcca ataatccggc tggtattttg
     1801 aaaagaaaag cccgattgaa acataaactg tttgatgtgg tagcagacca aaccggaaag
     1861 ttatttaaag atgatgtact cacgatagtt tgggccagac gttttgcgga atacaaacgc
     1921 ccggatttaa tcacacgcga ccatgaccgt tttagacgat taatggataa taaggagttt
     1981 ccaatacagc tcatctttgc cggaaaacct tatccaatgg attatggtgc tatcaatacc
     2041 tttaattcct tggcctattt aagtcggtca tttgataaca tggctgtttt aaccggatat
     2101 gagttaaaac tatcccgtca attaaaagaa ggttcagatg tttggttgaa tacaccaaga
     2161 gttacgcgtg aagcctcagg tactagtgga atgtctgccg ccatgaatgg ttctatcaat
     2221 ctatctacta atgatggttg gatttgtgag ttttatgata atggagtcaa ttcgtttgtg
     2281 ttgccaacag ttgatcatca tttgccaacg tatgagcagg atgccaagga tttagataat
     2341 ttattaacct tattggaaaa tgtagttgtt ccgatgtatt ataaaaagcg gaaggactgg
     2401 aataaaatca tgattgaaag catgaataca gtggtgccgt tttttgattc ggccagaatg
     2461 gtggatgaat attatgagaa gatgtatagt tgaacgtttt aagtagttga aagttctgat
     2521 tgcgaaagag ttagttttag gacaaaaaaa agtctgatat ttagccataa aacgtttgca
     2581 gaaacaaaaa atcgttctat atttgcactc gcaatcagaa aatgattgtg ttttattgga
     2641 gaaatggcag agtggtcgat tgcggcagtc ttgaaaactg ttgactgtaa caggtccggg
     2701 ggttcgaatc cctctttctc cgc
//
LOCUS       DAOTCW010000224         2682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_31968_length_2682_cov_28.967263, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000224 DAOTCW010000000
VERSION     DAOTCW010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31968_length_2682_cov_28.967263"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="P5335_11635"
     CDS             <1..189
                     /locus_tag="P5335_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRZ75576.1"
                     /translation="HSISEVVNALIENGLTIHLLNEYDYSPYNCFNGMEEFETGKFRI
                     QTFGNKIPMVYSIMASKK"
     gene            221..898
                     /gene="trmD"
                     /locus_tag="P5335_11640"
     CDS             221..898
                     /gene="trmD"
                     /locus_tag="P5335_11640"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRZ75577.1"
                     /translation="MRIDIITVLPELLKSPFEASILKRAIDKGLAEVHVHNLRDYTTN
                     KQKSVDDYQFGGGAGMVISIQPIDDCIAKLKSERKYDEIIYMSPDGETLNQKMANSMS
                     LLNNIIILCGHYKGVDQRVRDQFITKEISIGDYVLSGGELGAAVLSDAIIRLIPGVLS
                     NETSALTDSFQDNLLSGPIYTRPAEYKGWKVPDVLLSGNFAEIDKWRENKAYEHTKNR
                     RPDLLEE"
     gene            901..1914
                     /locus_tag="P5335_11645"
     CDS             901..1914
                     /locus_tag="P5335_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRZ75578.1"
                     /translation="MKPIKTALLAYGLSGKIFHAPFLATNEGFELYAVLERKSKKAKL
                     DYAEIKSYTSLEELLADDLIELVVVNTPNNTHFELAKAVLLAKKHVLLEKPATTTPEE
                     FKELLVLANSVYKKVFVYQNRRWSSDFCSTKEVINSGKLGDIVEAHLRFDRFRPTIGL
                     KAFKEKELPGSGILYDLGAHLIDQAISIFGKPKKHYIQKGMYRNGTLVDDYAFVHLLF
                     ENQVNAFITVSFHVLDIQPGIVIHGTKGSFIKEFCDEQENQLMMGISPLVEIFGFEKD
                     NKVGKLTFVNDKNEIVTEFIPSKKGNFNGLFEEVFNCIRNQKEFSVKDDEIIWQLKLL
                     SNE"
     gene            2034..2384
                     /gene="rplS"
                     /locus_tag="P5335_11650"
     CDS             2034..2384
                     /gene="rplS"
                     /locus_tag="P5335_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRZ75579.1"
                     /translation="MADLMKFVESEFVTKKDFPEFGAGDTITVYYEIKEGEKTRTQFF
                     KGVVIQRRGSGATETFTIRKMSGAVGVERIFPVNMPALQKVEVNQRGKVRRARIFYFR
                     DLTGKKAKIKERRR"
     gene            2535..>2682
                     /locus_tag="P5335_11655"
     CDS             2535..>2682
                     /locus_tag="P5335_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75580.1"
                     /translation="MNDILQTPIEYLKGVGPSRGEILRKELSIHRYGDLLTFFPNRYI
                     DRTRY"
BASE COUNT          944 a          417 c          491 g          830 t
ORIGIN      
        1 cactccattt cagaagttgt aaatgcattg attgaaaatg gtttaaccat ccatttacta
       61 aatgaatatg attattcacc ctacaattgt tttaatggca tggaagagtt tgaaaccggt
      121 aaatttcgga ttcaaacctt tgggaataaa ataccaatgg tttattcaat catggcatct
      181 aaaaagtaaa ccttatcttt gcctttctta aaaaacaaaa atgcgaatag atattatcac
      241 cgttttaccc gaacttttaa aaagtccttt tgaagcctca atcttaaaaa gagccattga
      301 taaaggattg gcagaagttc atgtgcacaa tttgagagat tacaccacca ataagcaaaa
      361 aagtgtagat gattatcagt ttggtggcgg tgcaggaatg gtgatatcga ttcaaccgat
      421 agatgattgc attgccaaat taaaaagtga acgcaaatat gatgaaataa tttatatgtc
      481 cccagatggt gaaacattaa accaaaaaat ggccaattcg atgtcgcttt taaataatat
      541 catcatttta tgtggacatt ataaaggtgt agaccaacgg gttcgcgacc aattcataac
      601 caaggaaatt tctattggtg attatgtgct ttccggtggt gaattgggag ctgctgtttt
      661 aagtgatgca attattcgct tgatacccgg agttttgagt aatgaaactt ctgccttgac
      721 agatagtttt caggataatt tgttatcggg acctatttat actcgtcctg ctgaatacaa
      781 aggttggaaa gtacccgatg ttttattaag tggaaatttt gcagaaattg acaaatggcg
      841 agagaataaa gcatatgagc atactaaaaa tcgtcgccca gatttactgg aggaataatc
      901 atgaaaccca tcaaaacagc ccttttagcc tacggacttt caggcaaaat atttcacgct
      961 ccatttttgg caaccaatga agggtttgaa ttatatgcag ttttagaaag aaagagtaaa
     1021 aaagccaagc tagattatgc agaaattaaa agttatactt ccttagaaga acttttagcc
     1081 gatgatttga ttgaattagt tgttgtaaat acaccaaaca acacccattt cgaactagca
     1141 aaagcagttt tactggcaaa gaagcatgtg ctcttagaaa aaccagccac aaccactccg
     1201 gaagaattta aagaattgtt agtcctagcg aattcagttt ataaaaaggt ttttgtttac
     1261 caaaatcgaa gatggagtag cgatttttgt tctacaaaag aggttatcaa ctccggaaaa
     1321 ttaggtgaca ttgtagaagc tcatttaaga ttcgatcgat ttcgtccaac tattggatta
     1381 aaagccttca aagaaaaaga attaccggga agtggtattt tatatgattt gggtgcacac
     1441 ttaattgatc aagcgatttc aatttttgga aaaccaaaaa agcattacat acaaaaagga
     1501 atgtatcgaa acggcacctt ggttgatgat tatgcgtttg tgcatctttt gtttgaaaat
     1561 caggtcaatg cttttataac tgttagtttt catgtacttg acattcaacc cgggattgtc
     1621 attcatggca caaaaggaag ttttatcaaa gagttttgtg atgaacagga aaatcaattg
     1681 atgatgggga tttcaccatt agtcgaaatc tttggttttg aaaaagataa taaagtagga
     1741 aaactaactt ttgtgaatga taaaaatgaa atagtaacag aattcattcc ttctaaaaaa
     1801 ggaaatttta acggattgtt tgaggaagta tttaattgca ttcgaaatca aaaagaattt
     1861 tcagtgaagg atgatgaaat catttggcaa ttaaaattac taagcaatga ataaaagtag
     1921 actattttgc tcgaaattta aaaatattta ttacttttgc acccacattt agaccaacct
     1981 ctgacgaaaa tcgtgaatgt tgctctgata atcattattt taacttaatt gttatggcag
     2041 atttaatgaa attcgtagaa agcgaatttg taacaaaaaa agattttcca gaatttggag
     2101 ctggtgatac tatcactgtt tattatgaaa ttaaagaggg tgaaaaaacc agaacgcagt
     2161 tcttcaaagg agttgtaatc caaagaagag gttcaggagc tactgaaacc tttactattc
     2221 gtaaaatgtc aggtgcagtt ggggtggaaa gaattttccc tgtaaacatg cctgctcttc
     2281 aaaaagtaga agttaaccaa agaggtaaag ttcgtagagc tagaattttc tacttcagag
     2341 accttacagg taaaaaagca aaaatcaaag aaagaagaag ataatctttt ttcgagttat
     2401 accaaatccc gttttgaaat tcaaaacggg attttttatt tattcttggc gacaaaatta
     2461 ttctctttca attttcatct caaaaaacta tcttcgcttt gaattgctgt tcacattaca
     2521 attcattttt aagaatgaac gatatcctac aaactcctat cgaatacctt aagggcgttg
     2581 gtccttctcg aggcgaaata cttcgaaaag aactcagcat tcatcgatat ggagatttgt
     2641 taactttttt cccaaatcgc tatatcgacc gtacacgata tt
//
LOCUS       DAOTCW010000225         2674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_32133_length_2674_cov_29.294387, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000225 DAOTCW010000000
VERSION     DAOTCW010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32133_length_2674_cov_29.294387"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..1013)
                     /locus_tag="P5335_11660"
     CDS             complement(318..1013)
                     /locus_tag="P5335_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRZ75581.1"
                     /translation="MKNIKITIVAIVLLIAFPFTGCKSVQNANNTQKGVAIGATTGAI
                     IGGILGNNLGKGGNTAMGAVIGGVVGGAAGGVIGNKMDKQAREIEEALPGAEVERVGE
                     GIVLVLGENSVNFDLNKSTLTSTAKANLDKLVPVFQSYADTDIVIYGYTDSTGRVEYN
                     QTLSEQRAASVRTYLASKGLSGSRIKTKGMGVNDPIASNDTPEGRSKNRRVEFTITAN
                     EKMIQDAEKEVKN"
     gene            complement(1018..1485)
                     /locus_tag="P5335_11665"
     CDS             complement(1018..1485)
                     /locus_tag="P5335_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRZ75582.1"
                     /translation="MKKILFIGLLSLIVFACKSNPAKQLDNKTEAGMKGTWTISSVTY
                     PGSDYIKVKSFEIADSQCFVGSTWNFVSNNNKGDVTISKSGCTSFTSPITWYINLEGN
                     FVMKVLNADEKAKKVRSGYVLKLANQTDNSFQLVDRINVGGTLTDVVYQFIKQ"
     gene            1648..>2674
                     /gene="htpG"
                     /locus_tag="P5335_11670"
     CDS             1648..>2674
                     /gene="htpG"
                     /locus_tag="P5335_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRZ75583.1"
                     /translation="MTTGKINVSVENIFPLIKKFLYSDHEIFLRELISNATDATLKLK
                     HLTSIGEAKVEYGHPKIEVRIDKENKKLHIIDQGLGMTAEEVEKYINQVAFSGAEEFL
                     EKYKDSAKDSGIIGHFGLGFYSAFMVASKVEIITKSYKDEPAAHWTCDGSPEFTLVPH
                     DKTDRGTEIILHIAEDSLEFLEDFKIRELLKKYNKFMPVPIKFGTKKETLPVPEGAAE
                     DVKPEEIEVDNIINNPTPAWTKAPTELKDEDYKEFYRELYPMQFEDPLFSIHLNVDYP
                     FNLTGILYFPKMSADLQIQKDKIQLYQNQVYVTDNVEGIVPEFLVMLRGVIDSPDIPL
                     NVSRSYLQ"
BASE COUNT          867 a          490 c          431 g          886 t
ORIGIN      
        1 tcaatcgaac cgtagaacga ataaaaatca ctttcaataa ttttgttctc ttggcaaaaa
       61 aggaatacat ttttgggttc tgcattattt tgcaaaacct ggtttatata taattcaaca
      121 agttggttgt cgtccaatgc cttcttttta gttgctgctt tttttactac ggtcattttg
      181 tttgatttag taatctgtta caaagatata tatttgttta atttttatga aaaatattta
      241 aacaaaaatt tgttaaagtt ttgacataaa aaaaggctct caaattgaga acctttttac
      301 aaaaattatt aagattttta atttttcact tctttttcag catcttgaat cattttctca
      361 tttgcagtga tagtaaactc tacccgacga tttttagatc ttccttcggg agtatcattg
      421 ctggcaatag gatcattgac ccccattcct ttggttttaa ttctactacc actcaaccct
      481 ttggatgcta aataagttcg aaccgaagca gctctttgtt cagataaagt ctgattatat
      541 tcaacacgac ctgtactgtc cgtgtaaccg taaatcacaa tatcggtatc cgcataactc
      601 tgaaaaaccg gaaccaattt gtctaaattt gcttttgctg ttgatgttag ggtagattta
      661 tttaaatcaa aattaactga gttttcaccc aaaaccaaaa cgattccttc tcctacacgt
      721 tctacttcag cacctggtaa agcttcttca atttcacggg cttgtttgtc cattttgttg
      781 ccaataactc ctccggcagc tccaccaact acaccgccaa ttacagctcc catagcagta
      841 tttccacctt taccaagatt attccctaaa attccaccta taatagctcc agtagtggca
      901 ccaatggcca cacctttttg cgtattatta gcattttgaa ccgatttaca acctgtaaaa
      961 ggaaatgcaa ttaaaagtac aattgcaaca attgttattt ttatgttttt catgttctta
     1021 ttgttttata aattgataaa ctacatctgt taaagttcct cctacattga ttcgatccac
     1081 caattgaaat gaattatccg tttgattggc taatttcaaa acataaccac tgcgaacttt
     1141 ttttgctttt tcatcggcat tcaaaacttt catgacaaag tttccttcca agttgatgta
     1201 ccatgtgata ggcgaagtaa aacttgtaca accggatttc gaaatagtta catctccttt
     1261 attattgttt gaaacaaaat tccaagtgct tccaacaaaa cattgtgagt cggctatttc
     1321 aaaagatttt actttgatat aatccgaacc gggataagta acagaactaa tggtccaagt
     1381 gcctttcatt cctgcttcgg ttttgttgtc caattgtttc gccggattgg atttacaggc
     1441 gaatacaatc aaggaaagta atcctataaa gagtattttt ttcatcgtga attaaattat
     1501 tagattcaac acaaagataa gaaatagaat ttttagaatt atttaaatag tttatataat
     1561 tcacacgtca atttgtcaga ttcttagttt tggtacttat tttgcaagag ttgtttctac
     1621 aaatgtttaa ttaaaaaata gctaattatg acaacaggaa aaattaatgt ttctgtagag
     1681 aacatctttc ccttaatcaa aaagttttta tacagtgatc acgaaatttt tcttcgtgaa
     1741 ttgatttcta acgcaaccga tgcaacctta aaactgaaac acctaaccag tattggagag
     1801 gcaaaagtag aatatggcca tccgaaaatt gaagttagaa tcgacaaaga aaacaaaaaa
     1861 ctacacataa ttgaccaagg attggggatg acagccgaag aggtagaaaa atacatcaac
     1921 caagtcgctt tttccggtgc ggaggaattc ttggaaaaat acaaagattc tgctaaagat
     1981 tcaggcatca ttggtcactt tggtttagga ttttattcgg cttttatggt cgcttccaaa
     2041 gtggaaatca tcaccaaatc atacaaagac gaacctgctg ctcattggac atgtgacggt
     2101 agtccggaat ttactttggt tcctcatgac aaaactgaca gaggaacgga aataatcttg
     2161 cacattgccg aagattcatt agagttttta gaagatttta aaattcgtga attgttgaaa
     2221 aaatacaaca aattcatgcc ggtgcctatt aaattcggaa ccaaaaaaga aacacttccc
     2281 gttcctgaag gtgctgcaga ggatgtaaaa ccggaagaaa tagaagtgga caacatcatc
     2341 aacaacccga caccagcttg gaccaaagct ccaacggaat tgaaggatga agattataaa
     2401 gaattttacc gcgaattgta cccaatgcaa tttgaagatc cgttgttcag cattcatttg
     2461 aacgttgatt atccgtttaa tttgactgga attttatatt tccctaaaat gtcggcggat
     2521 ttacaaattc agaaagacaa aattcaattg tatcaaaatc aggtttatgt aaccgataat
     2581 gtagaaggaa ttgtacctga atttttagtc atgcttcgcg gtgtgattga ttcaccggat
     2641 attccattga atgtttcgcg ttcctattta caat
//
LOCUS       DAOTCW010000226         2644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_32709_length_2644_cov_30.088065, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000226 DAOTCW010000000
VERSION     DAOTCW010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32709_length_2644_cov_30.088065"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1553)
                     /locus_tag="P5335_11675"
     CDS             complement(129..1553)
                     /locus_tag="P5335_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRZ75584.1"
                     /translation="MKILLTGANGYVGRRLFPELLSAGHEIVCCVRDKNRLGLDKTTL
                     DAITIWEVDFLEAIHFENCPTTIDVAYYLIHSMSSSTQIFDTMESKAAENFNSYMNYI
                     GVKQVIYLSGIVNNNTLSKHLQSRKNVEDILYQGNFNVTVLRAGIIVGSGSSSFEIIR
                     DLCEKLPLMITPKWVLTKAQPIAIRDVIQYLMGVLLKEECYNDSFDIGGPNVLTYKQM
                     MQLYAKTRGFKNWIYTVPIMTPKLSSYWLYFVTSTSYKLAMNLVDSMKMEVICSDNRL
                     QKMLGIKPISYTEAIKLAFIKIEQNLVISSWKDSLVSGRFKHNLSEFIQVPTFGCLKD
                     EKKLKILNPEKVLDNIWTIGGEKGWYYGNWMWKIRGFVDKLLGGVGLRRGRTHPSHLD
                     NGDSLDFWRVLLANKEEKRLLLFAEMRLPGEAWLEFRIDEENVLHQIATFRPRGLLGR
                     LYWYSLVPFHYFIFGGMIRNIGKG"
     gene            complement(1601..2224)
                     /locus_tag="P5335_11680"
     CDS             complement(1601..2224)
                     /locus_tag="P5335_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family NAAT transporter"
                     /protein_id="HRZ75585.1"
                     /translation="MELFIYIFAALFSVLNPLGAVPIFVGLTQDYTKVERSRVSLLTA
                     LNVFIILIISFFIGEYVLKFFGISIDALRIAGGLIIVSSGFSLLTGNFNKTRGVNKKV
                     ESDAQTRNQIALTPLAIPMLAGPGSISLLIAFYQDYTEISEKIIAVLAILGVALVIFF
                     VLKSAHYLSRILGASGIVAISRIIGFIVIAIGVQYISSSVVNIFKTI"
     gene            complement(2290..>2644)
                     /locus_tag="P5335_11685"
     CDS             complement(2290..>2644)
                     /locus_tag="P5335_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75586.1"
                     /translation="LIDNIKLNGIGSPRLDIIKTSEEIATLLSYDNNRNFCNVELRPK
                     GIIIRFRSLLETFSLIIPFYKLVIFKPANSFTLHIDHHFISIDAPPKNKSVNSFMEKL
                     NQFKAENSPMYIDDL"
BASE COUNT          894 a          489 c          431 g          830 t
ORIGIN      
        1 aagcttgtgg cggtggaagt gcccgttgta tgatggcgga ggtttttttg ccaaaagctg
       61 aataaatgtt gaaaaaatct ttgtcaaagt tcaaaacttt gacaaagatt ttttcaacat
      121 ttaacccatt acccttttcc aatattccga atcatcccac caaaaataaa atagtgaaac
      181 gggactaagc tataccaata caatcttccc aaaagtcctc ttggtctaaa agtggcgatt
      241 tgatgtaata cattttcttc atcaatccgg aattccaacc aagcctcacc gggtaatcgc
      301 atttcggcaa aaagtaagag ccgcttttcc tctttgttgg ccaacaatac ccgccaaaaa
      361 tctaaggaat caccattgtc taaatgggat ggatgagttc gtcctcttcg taagcctacg
      421 cctcctaaca atttatctac aaaaccacga attttccaca tccaattgcc gtaataccaa
      481 cctttttcac caccaatggt ccagatgtta tctaatactt tttccggatt tagtattttt
      541 aatttcttct catcttttaa acaaccaaaa gtgggcactt gtataaactc cgacaaattg
      601 tgtttaaacc gtccactcac caaactgtct ttccaacttg agatgactag gttttgttca
      661 attttaatga aagctaattt aatagcttcg gtatatgata tgggtttgat gcccagcatt
      721 ttctgaagtc tgttatcact acaaatcact tccattttca tactatccac taaattcata
      781 gccaatttat aggaagtaga ggtcacaaaa tataaccaat aggaagatag ttttggcgtc
      841 ataatcggaa ccgtataaat ccagttttta aagcccctgg tttttgcata caactgcatc
      901 atttgtttgt aagtcagcac attaggtccg ccaatatcaa aggagtcgtt gtagcattct
      961 tcttttaaca agacacccat taaatattga ataacatcac gtatcgctat aggttgtgct
     1021 tttgttaaaa cccattttgg agtaatcatc aagggcaatt tttcacacag gtcgcgtatg
     1081 atttcaaatg atgaacttcc ggaacctacg attattcctg cacgcaaaac ggttacatta
     1141 aaatttcctt ggtacaaaat atcttccaca tttttacgcg actgcaggtg ttttgaaagc
     1201 gtattgttat tgacgatacc acttagatag atgacttgtt ttacgccaat gtaattcata
     1261 taactattga aattttctgc agctttcgat tccatcgtgt caaaaatttg ggtagatgaa
     1321 gacatggaat gaataagata ataggcaacg tcaatagttg taggacagtt ttcaaaatga
     1381 attgcttcca aaaaatctac ttcccaaatg gtaatggcat ccaaagttgt tttgtctaat
     1441 cctaagcgat ttttatcgcg aacacagcat acaatttcat ggccagctga taaaagttcg
     1501 gggaataatc gacgaccaac ataaccattg gctccggtaa gtagaatttt catatccgct
     1561 attttcctta aaggtactaa gaaaagcgat tgaacaagcg ttaaattgtt ttgaaaatat
     1621 tgacaacgga gctgctgatg tattgtacgc caatggcaat tacaataaat ccgataattc
     1681 ttgaaatagc tactataccg gatgctccta aaattctaga aaggtaatgt gcacttttta
     1741 aaacaaagaa aatcaccaat gctacaccta aaatagccaa aacagcaata attttttcac
     1801 ttatttcggt ataatcttga taaaaggcaa tcaaaagtga aattgaaccg ggacctgcta
     1861 gcattggaat tgctaatgga gttaaagcaa tttgattacg ggtttgggca tcactttcaa
     1921 cttttttatt gactccacga gttttgttaa aatttccggt gagcaatgaa aaaccggaac
     1981 ttacaataat taaaccacct gcaatacgaa gtgcatcgat gctaatgcca aaaaatttca
     2041 acacatattc gcctataaaa aaggagatga ttaaaatgat aaacacattt aatgcggtca
     2101 acaatgaaac tctcgaacgt tcaactttgg tgtaatcttg tgttagcccc acaaaaattg
     2161 gcacggcacc taatggattt aaaaccgaaa ataatgctgc gaaaatgtaa ataaatagtt
     2221 ccatcgtatt ttgcaaaaat aaaaacaact tagtttttgg tagattacca aatcaataaa
     2281 acaaagttac tataaatcgt caatatacat aggagaattt tcagctttaa attgatttaa
     2341 tttttccatg aaactattta cacttttatt ttttggagga gcatcaatag aaataaaatg
     2401 atgatcaatg tgaagagtaa aagaattggc tggtttgaaa attactaatt tataaaatgg
     2461 aatgattaaa gaaaatgttt ccaacaatga cctgaaacgt ataatgattc cttttggtct
     2521 caattctaca ttacaaaaat tccggttgtt gtcataagaa agtagtgtgg caatttcttc
     2581 actcgttttg ataatatcta atcggggtga accaattccg tttagtttaa tattgtcaat
     2641 taaa
//
LOCUS       DAOTCW010000227         2628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_32989_length_2628_cov_25.214147, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000227 DAOTCW010000000
VERSION     DAOTCW010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32989_length_2628_cov_25.214147"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="P5335_11690"
     CDS             <1..1190
                     /locus_tag="P5335_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75587.1"
                     /translation="QEIQPAKTFYSNNGATFKFRVYNYPPTEGDNNNNNNHTADVNKI
                     IENNGSISVISKYTVPSIFLQGYGAVLNAQGDYVSPQSSTAVVIQNSSFNDDFEVDNN
                     NSRLVSYNYSGGAHGSYGFIGARIYVHNPTFFSGAISASSNQGYMHTLNDSNFMLSMG
                     YNSNYGKPMFYRYNPSNYTWIGDLVTQMDYVQGSGVNIPMTNDASKVGNSDKVYWAWL
                     SYTTNLSNGKINIISYDGSSFSAISAMDGIGSIGSGWDSVHTITLFKNPNNLGSPYIV
                     VRRYNTDVLDIYKFNGSAIEVVKTGVAIPAAIPILSGSTRVFKDLVFTGNNVYMITGM
                     DKNLYKLNGNSFEIDRPNLTVEQERITSIEGNSNGLLLAISKTLNTQPQPKTVSDVIL
                     LPN"
     gene            1201..1722
                     /locus_tag="P5335_11695"
     CDS             1201..1722
                     /locus_tag="P5335_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75588.1"
                     /translation="MKKFSITILLLFVLSSLTIKAQSNNQITYMLLLNEAKQNFATII
                     GDQFNKSEDGSKTFHSCKDGFLTTTEFITVDNNNGKQKSYYTLYFNLSELNSDDLIHV
                     SKIVEDAIGFANLLVSGGKYKGEDINNPETNGVTTRLKDTATENAVMEITSGKYNGSQ
                     MVMITFFGANFYN"
     gene            1729..2628
                     /locus_tag="P5335_11700"
     CDS             1729..2628
                     /locus_tag="P5335_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75589.1"
                     /translation="MKNILLLVIFFSIVSKAQNEKYKTADYALPTNVKSYNTFIYLYD
                     VASGKFKTVEKKLLFFENGLLKTDFVMDNYLGLYAKLRTYEYNDQKQLIAIKEASDYQ
                     NPKYYLSREFFYTQNQLTKVIDHGGSKKIIKELTYDKLGKVIQSKTFDEDNVLIEEVR
                     YNYTTPSDYSVISKIFSNGKEISLIEDKYLKNLLISKKINDSLGKILYQYEYDSKGNQ
                     ILLDDGLEEYHTDYSYGQTGAILKSRATEYDEFEDSLSNFFKFSEITFTDGTKEGSKN
                     LDVEFVKSFNMSVLSYEVVDDLR"
BASE COUNT          935 a          382 c          454 g          857 t
ORIGIN      
        1 ctcaagaaat tcaacccgct aaaactttct actccaataa cggggcaact tttaaattca
       61 gagtgtataa ttatccacca actgagggtg ataacaataa taataacaac catacagctg
      121 atgttaataa aattattgaa aataacggtt caatttcagt catttcaaaa tatacagttc
      181 cgtctatttt tttgcaggga tacggagctg ttctcaatgc tcaaggtgat tatgtttcgc
      241 cgcaatcttc cacagcagta gttattcaaa attccagttt taatgatgat tttgaagtgg
      301 ataacaacaa ttcaagattg gtttcctata attattccgg tggtgctcac ggatcctatg
      361 gttttatcgg tgcaagaatc tatgtgcaca atccgacttt tttttcagga gccatttctg
      421 ccagttctaa tcaaggatac atgcatacct tgaatgacag taattttatg ttgagtatgg
      481 ggtataattc caattatggt aaaccaatgt tttatcgtta taatccgtca aactacactt
      541 ggataggtga tttggttact caaatggatt atgttcaagg aagtggagtg aatattccta
      601 tgacaaatga cgcttctaaa gttgggaata gtgacaaagt ttattgggct tggttaagtt
      661 atacaactaa tctttctaac ggaaaaatta atattattag ttatgacgga agttcattca
      721 gtgctatttc agcaatggat ggaattggaa gtattggttc aggatgggat tcagttcata
      781 ccataacttt atttaaaaac cctaataatt tagggagtcc ttatatagtc gtaagaagat
      841 acaataccga tgttctggat atatataagt ttaatggttc agctattgaa gtggttaaaa
      901 caggggttgc cattccagct gctattccta ttctttccgg ttctacaagg gtttttaaag
      961 atttagtatt taccggaaac aatgtttata tgattaccgg aatggacaaa aatctatata
     1021 aattaaatgg caattctttt gaaattgatc gacctaattt aaccgttgaa caagagcgaa
     1081 tcacctcaat tgaaggaaat tcaaacggac ttttactggc tatttctaaa acattaaata
     1141 cacaacctca gcctaaaact gtttcagacg ttattttact tcctaactaa attctaaatc
     1201 atgaaaaaat tttcaatcac gatcctattg ttatttgttt tatcaagttt aacaatcaaa
     1261 gctcaatcca acaatcagat aacctatatg ttattattaa atgaagcaaa gcagaatttt
     1321 gccactatta ttggtgatca gtttaacaaa agcgaagatg gttccaaaac ctttcattcc
     1381 tgtaaagatg gttttcttac cacaactgaa ttcatcacag tagataacaa taatggaaaa
     1441 caaaaatcgt attataccct ttatttcaac ttgtctgagt tgaatagcga cgatttaatt
     1501 catgtttcca aaattgttga agatgctatt ggttttgcca atcttttggt ttccggtgga
     1561 aaatataaag gagaagatat caataatcct gaaaccaatg gagttacgac ccgattgaaa
     1621 gatacagcca ctgaaaatgc ggttatggaa ataacttcgg gaaaatacaa tggttctcaa
     1681 atggtgatga ttactttttt tggtgctaat ttttataatt aatgtaatat gaaaaacata
     1741 ctcttactag tcatattctt ttcaatcgtg agtaaggcac aaaatgaaaa atacaaaaca
     1801 gcggattatg cacttcctac gaatgtaaaa agttataata cctttattta cttatacgat
     1861 gttgcatcgg gaaagtttaa aacagttgaa aaaaaactgt tattttttga aaacggattg
     1921 ttgaaaaccg attttgtgat ggataattat ttgggtttgt atgccaaatt aagaacgtat
     1981 gagtacaacg atcaaaagca attgattgcc ataaaagagg catctgatta tcaaaatccg
     2041 aagtattatt taagtagaga atttttttat acccaaaatc aattgacaaa agttattgat
     2101 catggcggga gtaaaaaaat aataaaagaa ttgacgtatg ataaattggg aaaagttatt
     2161 caatcaaaaa catttgatga agataatgta ctaattgaag aagtgagata taattatacc
     2221 accccatccg attatagtgt aatttcaaag attttttcaa acggaaaaga aatttcattg
     2281 atcgaagata agtatttgaa aaatctcttg atatcaaaaa aaatcaatga ttctttaggt
     2341 aaaattttgt atcaatatga atatgattcc aaaggaaacc aaatactttt agatgatggt
     2401 ttagaagaat atcacacgga ttattcgtat ggacaaacag gagccatttt gaaaagcaga
     2461 gcaacagagt atgacgaatt tgaagattcg ttgagtaatt tctttaaatt ctccgaaatt
     2521 acgtttacag acggaacgaa agaaggttca aaaaacttag atgtagaatt tgtcaaatca
     2581 tttaatatga gtgtgctttc gtatgaagtg gtagatgatt tgagatag
//
LOCUS       DAOTCW010000228         2621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_33122_length_2621_cov_30.350351, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000228 DAOTCW010000000
VERSION     DAOTCW010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33122_length_2621_cov_30.350351"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="P5335_11705"
     CDS             <1..1437
                     /locus_tag="P5335_11705"
                     /inference="COORDINATES: protein motif:HMM:NF025478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector amidase Tae4 family protein"
                     /protein_id="HRZ75590.1"
                     /translation="KVILDSISYSDGSFDNLVVQIDNDQKIEAFLIHYKPDIDILYNP
                     DHKTYTFEGSTSVSKVYGITSFLGNGLGDNGEDNGTNNTSEGAGGNDTYNSIICVTLL
                     LCDYEYTHIAGPGCKNTYTRTECYSNPNYNPWSGYGNAPADETDLNNDNNNNGPGGGG
                     LGSGNNTNNNQNSSFTTATVFTEELDDMNLQMQILREKNFREALNWNQKTWLNNNSNI
                     ETEIIDYLESQVENVIEDTGYPDEEINFIEELINLAIQYPTMYTYDNLMNWFFTTREG
                     IDPISYDNNFWEDPNLSFPLQSLPSWSDFDIAYPRVDGSELVTIVGGAVQIAYNQYPN
                     LSRGYCALKVSRALNYSGIIIPQIITTSGNPGTLQGSDGKYYFLNAKALNKWMRETFG
                     TNPATNTSPLNLNHFHFTSNQGGINGLNFPNLMIGLKGIYSMVSTNPNWASGHADLID
                     ENGICVFGCHLNDSPPAPIDYIDIWVLE"
     gene            1455..1883
                     /locus_tag="P5335_11710"
     CDS             1455..1883
                     /locus_tag="P5335_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75591.1"
                     /translation="MKKSIIKLFFIFFILISCNSIHVKDGFLYEENNSKEIWINSYKY
                     EVFYECIKQGIGNDSLRIILKNKDLFNKSQELEFNTIDSARILGHKIIINLPEPYIKI
                     DEGSINKNYISYNCLKYYASKELDEIAMIEYKKHFSKKRK"
BASE COUNT         1012 a          300 c          417 g          892 t
ORIGIN      
        1 aaagtgattt tagacagtat ttcttattca gatggctcat ttgataatct tgttgtacaa
       61 atagataatg atcaaaaaat tgaagcgttt ttaattcatt ataagccaga tattgatatt
      121 ttatataatc cagatcataa aacatatact tttgagggtt caactagcgt aagtaaagtt
      181 tatggaataa catcattttt aggaaatgga cttggtgata atggagaaga taatggaacg
      241 aataacacaa gtgaaggggc tggtggtaat gatacatata acagcattat ttgtgttact
      301 ctattacttt gtgattatga atatactcat attgcaggac cgggttgtaa aaacacttat
      361 acacgcacag aatgttattc aaatccaaat tataatccgt ggagtggtta tggtaatgca
      421 ccagcagacg aaaccgactt gaataatgat aataataata atggacctgg aggaggtggt
      481 ttgggctcag gtaataatac taataataat caaaattctt cattcacaac ggctacagtt
      541 tttacggaag aattagatga catgaattta caaatgcaaa ttttaagaga aaaaaacttc
      601 agagaagctt taaattggaa tcaaaaaact tggttaaaca acaattcaaa tattgaaaca
      661 gaaattattg attatttaga aagtcaagtt gaaaacgtga tagaagatac tggatatcct
      721 gatgaagaaa ttaattttat agaagaattg attaatttag caattcaata tccaacaatg
      781 tacacttatg ataatttaat gaattggttt tttactacaa gagaggggat tgatccaatt
      841 tcatatgata ataatttttg ggaagaccct aatctttctt ttcctctgca atcattacca
      901 agctggtctg attttgatat tgcttatcca agagtagatg gctcagaatt agtcacaatt
      961 gtaggtggag cggttcaaat tgcttataat caatatccaa atctctctag aggctattgt
     1021 gctttaaaag tttcaagagc acttaattat tcagggataa ttatacctca aataataaca
     1081 acatcgggta atcctggcac attacaagga agtgacggaa aatattattt tttaaatgct
     1141 aaagcactta ataaatggat gagagaaact tttggaacca atccagcaac gaatacttca
     1201 ccattaaatc taaatcattt ccatttcact agtaatcaag gcgggataaa tggattaaat
     1261 tttccaaatt taatgattgg tcttaaagga atttattcta tggtttcaac caatccaaat
     1321 tgggcatctg gacatgctga tttaattgat gagaatggaa tttgtgtatt tggttgtcat
     1381 ttgaacgatt caccaccagc accaatagat tatatagata tttgggtgtt ggaataatta
     1441 ttaaatataa ttttatgaaa aaatcaatta ttaaactttt ttttattttt tttattttaa
     1501 tttcatgcaa ttcaattcat gtaaaagacg gttttttata cgaagaaaat aattcaaaag
     1561 aaatttggat aaattcttat aaatatgaag ttttttacga atgtataaag caaggtattg
     1621 gtaatgatag ccttagaatt attcttaaaa ataaagactt gtttaataaa tctcaagaat
     1681 tagaatttaa tactattgat agtgctagaa tattagggca taaaataatt attaatttgc
     1741 cagaacctta cataaaaatt gatgaaggct caattaataa aaattacatt tcgtataatt
     1801 gtttgaaata ttatgctagt aaagaattag atgaaattgc aatgattgaa tataaaaaac
     1861 attttagtaa aaaaagaaaa tgaatatagt taattgttaa aggaagaaag aatagatgtt
     1921 tgggtattat attaattttt aaattaatga aattcttata aacactaaag cttatgaaaa
     1981 tttaatattg tttactatac taataatatc ttcttgttct actacaaagg tattaagaac
     2041 agaatgagtg gaatttaaaa attgagataa aaaatttaaa gaaaggagat tttgcttgtg
     2101 tcagttaaat ggatatgaaa acaagaattt gaaaatgatt tatgtaagat tgataattcg
     2161 gtactataat ccgcttagaa ttgcaatctt agatgaagtt tagaatcatt taataaaagg
     2221 agaggttgaa aaaaaatcgt ttagactcaa taatgtctgt tggacactat ccggatggtc
     2281 agaaaaatct tctttaaaaa cgtacagtaa ttaatcattg tttagagttt tacattagta
     2341 aaagatttga tagtttaaca aaatttcaat agaaagattg gaaaaagatt ccaaatattt
     2401 tagataaaat acattctaaa atcccaactt tttaaacttg aatcaagttt tggatttaag
     2461 aagcaaatgt aatcttccgt aaattaacca cgatttaaaa taaatttact aactaaatcg
     2521 gtcgaatgcg atcagattat aactaaaaaa aaacctcaat tgaggttttt tttctaagta
     2581 tttttataca atctccagtg tttttgcttt gttaactaag t
//
LOCUS       DAOTCW010000229         2618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_33181_length_2618_cov_32.658993, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000229 DAOTCW010000000
VERSION     DAOTCW010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33181_length_2618_cov_32.658993"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="P5335_11715"
     CDS             complement(<1..704)
                     /locus_tag="P5335_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75592.1"
                     /translation="MKTQLPTHTFHIPVMGLAFTIDSPIRVAHYGIDSVISIADDELI
                     EKMNAFYAEKFQIPYQEITKKINDYRAKRITSYLNLVDKVVKEKFELYKTELAHSKTS
                     LEHFIAMLPTKSELKTKLDIFLSEGISCPSQLKTFLEKNLFPGSIDVNIMTKLDKDNF
                     DGNEQLPVEFNDAHAALRGFVNSTVESSLVLSAGMNPRLFTYMEQFDVFYPTADGFLK
                     KKIILKVSDYRSALIQG"
     gene            complement(886..1440)
                     /locus_tag="P5335_11720"
     CDS             complement(886..1440)
                     /locus_tag="P5335_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRZ75593.1"
                     /translation="MKKTIQLLVLAGSLLVLSCKQNNETPQAATPENGVDLSVGQEGV
                     VDESSNPNIVQVASGSKDHTTLVAAVKAAGLVTSLSNAGPFTVFAPTNAAFDKLPAGT
                     VDDLLKPEKKGDLENVLGYHTYVGSLKTEYMQNGQEFDMVYGGKVKITKEGDKTFVNG
                     SEIVASIPTSNGIIHVIGDVLLPK"
     gene            complement(1526..>2618)
                     /locus_tag="P5335_11725"
     CDS             complement(1526..>2618)
                     /locus_tag="P5335_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75594.1"
                     /translation="NIQHAHSHFAFAGWVTQLLMVLMVRYLSFYTSESKISKYNTLFW
                     INIIGAYIMLISFIIQGYGFISILFSTISIFVFVWFAIWFYKDSKEIPSHSLSKNWFL
                     VSLLFGVISSIGTFVLAYMMATKHVPQDLYLSSVYFYLHFQYNGWFWFACTGLFVSLL
                     QPNLTNTRENKTVFQLFAWSCLPAYLLSVLWLKLPVWVYALAVISAIVQVFAWFKLLK
                     FSLSIFKVNPRISAFLKILFKGIAFCVTLKLLLQFGSVFPAVSKLAFGFRPIVIAYLH
                     LVLLAIISGFLLVYLYTNELVNRSSKTVLFLVIFIVGIFLNEMLLALQGIFSFEYILI
                     PYANEMLFIVALILLIGISGIVFFNQKKV"
BASE COUNT          960 a          431 c          414 g          813 t
ORIGIN      
        1 ccttggatta aagccgaacg atagtcactt acttttaaaa taattttctt ttttaggaaa
       61 ccatcagctg ttggatagaa aacgtcaaat tgttccatat aagtgaacaa tcgcggattc
      121 atacctgccg ataaaaccag tgaagattct actgtagaat ttacaaatcc acgcaaagcc
      181 gcatgtgcat cattaaattc gacaggcaat tgttcatttc catcaaaatt atctttatcg
      241 agtttagtca tgatattgac gtcaatactt cccggaaata aatttttctc caaaaaggtt
      301 ttgagctgag atggacagga aatcccttcg gataaaaaaa tatctaattt ggtttttaat
      361 tcagattttg ttggcaacat tgcgatgaaa tgttctaaac tggttttgct gtgggctaat
      421 tctgttttgt ataattcaaa tttttctttc acaaccttgt ccaccaaatt caaataggag
      481 gtgattcttt tagcacgata atcatttatt ttttttgtta tttcctgata gggaatttga
      541 aatttttcag cataaaatgc attcattttt tcaattaatt cgtcgtctgc tatggaaata
      601 actgaatcga taccatagtg agccactcga atgggactgt caatagtaaa tgccaatccc
      661 attacaggaa tatgaaacgt atgagtagga agttgggttt tcattaaatg atatttatga
      721 tataaagtca caaagatgct ttttgtaata agagcaaaat atgattttaa tcatgtacat
      781 tcatatttaa caaaactata attggtattt ataaaaaaaa gcgtgaatac gaccattcac
      841 gctttttttc tgaattaaaa attatagtaa gaagaaaaac caaatttatt taggtaataa
      901 tacatcgcca attacatgaa taattccatt agaagttgga atactcgcca caatttcaga
      961 accatttaca aacgttttat caccctcttt tgtgatttta actttgcctc cataaaccat
     1021 atcaaattct tggccatttt gcatgtattc tgtttttaat gaacctacat aggtatgata
     1081 ccctaacaca ttttctaaat ctcctttttt ctccggtttt aataagtcat ctacagttcc
     1141 tgcaggaagt ttgtcaaaag cggcattggt aggagcaaag acagtaaaag gtcctgcatt
     1201 gcttaaagag gttaccaatc ctgcagcttt aacagcggca actaacgtgg tatgatcttt
     1261 actgccggaa gccacttgaa caatattcgg attagacgat tcatctacaa ctccttcttg
     1321 tccaacactt aaatccacac cattttcggg agtggcagct tgcggagttt cattgttttg
     1381 cttgcaactt aacaccagta gtgaaccggc taaaacgaga agttgaattg tttttttcat
     1441 gattaggata gtttaaaatt tctgattcaa agttcaagta tttaacaaat ctattttatg
     1501 agcgaaatca taagaatata tttttttaga cttttttttg attaaaaaat acgattcctg
     1561 aaatgcctat taagagaata agtgctacta taaaaagcat ttcgttggca tagggtatca
     1621 aaatatattc aaatgaaaat ataccttgaa gagccaaaag catttcgttt aaaaagatcc
     1681 ctacaatgaa aatcactaaa aaaagtactg tttttgaaga tcgattaacc aattcgttgg
     1741 tatataaata gaccaacaaa aacccactga taattgccaa taaaaccaaa tgaaggtaag
     1801 cgataacaat aggtctgaat ccaaaagcta atttactcac agcaggaaat acagaaccga
     1861 attgcaaaag taatttaagc gttacacaga aagcaattcc cttaaaaaga atttttaaaa
     1921 atgctgatat tcgtggattt actttaaaaa tagaaaggga aaatttaagt aatttaaacc
     1981 aagcaaatac ttgaacaatg gccgaaatta ccgcaagtgc ataaacccaa acgggtaatt
     2041 ttaaccataa aaccgatagc aaataggctg gaagacaact ccaggcaaat agttgaaata
     2101 ccgttttatt ttcgcgagta tttgtcaaat taggttggag taagcttaca aataatccgg
     2161 tgcaagcaaa ccaaaaccaa ccgttgtatt gaaaatgcaa atagaaataa acagaggata
     2221 aatataaatc ttgtggtaca tgtttcgttg ccatcatata agccaaaaca aacgtaccaa
     2281 ttgatgagat tacgccaaaa agtaaagaaa ctaaaaacca atttttactt aatgaatggg
     2341 agggaatttc tttagaatct ttgtaaaacc aaatagcaaa ccaaacaaat acaaaaatac
     2401 tgattgttga aaataggata gaaataaatc cataaccttg gattataaaa ctaatcagca
     2461 taatgtatgc tccaataata ttgatccaaa aaagtgtatt gtattttgaa atttttgatt
     2521 cagaagtata aaaggataaa taacgaacca ttaacaccat caacaattgc gtgacccaac
     2581 cggcaaaagc aaaatgtgaa tgagcatgtt ggatattt
//
LOCUS       DAOTCW010000230         2610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_33330_length_2610_cov_37.413699, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000230 DAOTCW010000000
VERSION     DAOTCW010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33330_length_2610_cov_37.413699"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..801
                     /locus_tag="P5335_11730"
     CDS             130..801
                     /locus_tag="P5335_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75595.1"
                     /translation="MKKYLFFIITLIFISCDEDKPIEAGIETRVSGHINDIFDAATPI
                     ENFKIKLGEYRRDFSFDGGAYDVFIKFIDSTITNNEGYYDLTFTTTGNGSRYKIHCEQ
                     TDQVWTIYQDPVLITNIGGENQIDFGFIHLYPVDLRITLTDVDNLPIQISGQLHPFSL
                     GRLDENNIEYIRRIYVSKLEGQEIRFYKTLADGTVQIAQFSFPATNSTILTEFNINLT
                     NADFN"
     gene            1112..2410
                     /locus_tag="P5335_11735"
     CDS             1112..2410
                     /locus_tag="P5335_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HRZ75596.1"
                     /translation="MISNFFDFIDLHNGEERKEKVAETIKTNISFRGSNLWVLACAIV
                     IASVGLNVNSTAVIIGAMLISPLMGPIVGAGFALAIYDFDLLKKSVKNLLIATVVSLF
                     VATIYFFFSPFKEIQSELLARTSPNIYDVLIAFFGGLVGVIALTRVEKGNPIPGVAIA
                     TALMPPLCTAGYGLAVMNLPFFLGAFYLYCINCFFICISTFLIIRYLKYPVVKILNTK
                     FEKLIKYGITVLILVLIIPSFYLAYNLFEEKKYRQNVEQFITNEFINNSYTLIFKQIK
                     FNSNPKKIELAFLTKKFTDEELKLLNQKLEQYDIPNTELNIKQDTKDLKGEILNELGN
                     QNKILSEKDILINTLQSELSEYKIDNTETIKEISILFPELKDVSIGRHIINPNTDSTK
                     TMTVLLYQTDDTNKEFDKNKIDQWLKQKFKTNEIKIVCQQ"
BASE COUNT          935 a          346 c          447 g          882 t
ORIGIN      
        1 gtggtaagta ttatttctta gctttaatgg gttgtgttta atttaattgg ttatttttga
       61 gggagcggtt tttagtaggg gggggattag acggggtgac attgtgtgca ataaataaaa
      121 aattatttta tgaaaaaata tcttttcttc attattactt taattttcat cagctgtgac
      181 gaggataaac ctattgaagc tggaattgaa actagagtat ccggccatat aaacgatata
      241 tttgatgctg ctactccaat agagaatttt aaaatcaagc taggcgagta taggagagat
      301 ttttcttttg atggtggtgc ctatgatgtt tttattaaat ttatcgattc tacaattact
      361 aacaatgaag gttattatga tttaacgttt acgacaacgg gaaacggttc aagatataaa
      421 attcactgcg aacaaactga tcaggtatgg acgatctatc aagatcctgt acttattaca
      481 aatattggtg gtgaaaatca aatcgatttt ggatttattc atttatatcc tgtagacctc
      541 agaattacac tgactgatgt agataactta cctatccaaa taagtggtca acttcatccg
      601 tttagtctgg gaaggctgga tgaaaataat attgaataca ttcggcgaat ctacgtatct
      661 aaacttgaag gacaggaaat cagattctat aagactcttg cagatggtac cgtgcaaatt
      721 gcccaatttt catttccagc gacgaattca accatcttaa cggaattcaa catcaacctt
      781 acgaatgctg actttaatta gttttactgc acgcaataga ggtaaccgtt gcacaactca
      841 ctaaaaaaac taaattttca tactaattat aaaaaacgac ctcgtttaga gctttctaaa
      901 cggggtttgt ttttgggtgg atgtgggtga ttttttgctg ttggttttcg gttagcattt
      961 tttttaagct aaccggtttg tgtttgattt aattggttaa ttttgaggga gcgatttagt
     1021 aggggtttga tgtgggttta cctgcaaccc taatgtagac tcaagaaagt gaaaaaaata
     1081 atcaatgtaa ttaaaattta aaaatatatt tatgattagt aatttttttg attttataga
     1141 ccttcataac ggcgaagaac gaaaagaaaa agttgcagaa acgattaaaa ccaatatatc
     1201 atttcgtggc tcaaaccttt gggttttggc ttgtgccatt gtcattgcgt cagttggatt
     1261 aaatgtaaat tcaacagctg taataattgg tgcaatgctt atatcaccgt taatgggacc
     1321 aattgtagga gcaggttttg cattagcaat ttacgatttt gacttactga aaaaatcagt
     1381 caaaaattta ttaattgcaa cagttgtgag tttatttgtt gcgacaattt actttttttt
     1441 cagccctttc aaagagatac aatctgaact attagctaga acatcaccaa atatttatga
     1501 tgtattaatt gcattttttg gaggattagt aggtgttatt gctttgacaa gagtagaaaa
     1561 aggaaatcca attccaggtg tggcaattgc aacggcatta atgccacctc tttgtacagc
     1621 aggatatggc ttagctgtaa tgaatttacc ttttttttta ggagcttttt atttgtattg
     1681 tatcaattgt ttttttattt gtatttcaac ttttttaata ataagatatc tcaaataccc
     1741 cgttgttaaa atattaaata ctaagtttga aaagctaatt aaatatggga ttacagtatt
     1801 aattttagtt ttgataattc cgagttttta tttggcttat aatctttttg aagaaaaaaa
     1861 gtatcgtcaa aatgtagagc aatttattac aaatgaattt attaacaata gctatacatt
     1921 aatttttaaa caaataaagt ttaattcaaa ccccaaaaaa atagaacttg catttttgac
     1981 aaaaaaattt actgatgaag aactgaaatt gttaaatcaa aaacttgaac aatatgatat
     2041 acctaacaca gaactcaata tcaaacaaga cacaaaagat ttaaaaggag aaatattgaa
     2101 tgaattagga aatcaaaata aaatattatc tgaaaaggac attttgataa atacccttca
     2161 aagtgaacta agcgaataca aaattgataa tacggaaaca ataaaagaga tttcaatatt
     2221 gtttcctgaa ttaaaagatg tatctattgg cagacacata ataaacccaa atacagactc
     2281 aacaaaaaca atgactgttt tactttatca aacagacgac acaaataaag aatttgataa
     2341 aaataaaata gaccaatggt taaaacaaaa atttaaaaca aatgaaataa aaatagtatg
     2401 tcaacaataa ggaaagcagg tagtacaagt agctattgta cagcccacta aaaaaactaa
     2461 attttcatac taattataaa aaacaacctc gtttagtgtt tgctaaacgg ggtttgtttt
     2521 tgggtggaag ttggtttaaa tagtaaaaaa tcttgcaaag gggcttttta cgtttcaaag
     2581 aaatttctaa gggtttttat tttgaaataa
//
LOCUS       DAOTCW010000231         2418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_37277_length_2418_cov_41.237833, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000231 DAOTCW010000000
VERSION     DAOTCW010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37277_length_2418_cov_41.237833"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="P5335_11740"
     CDS             complement(<1..195)
                     /locus_tag="P5335_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75597.1"
                     /translation="MSKCFFTLTLFFCFTFAISQNFVSINGKIIDDNTKLPLESATVY
                     LTSKKDSTVIDYTISGKNGNF"
     gene            complement(286..1077)
                     /locus_tag="P5335_11745"
     CDS             complement(286..1077)
                     /locus_tag="P5335_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRZ75598.1"
                     /translation="MVTIENKIVWITGASSGIGEGLAYALAQKNCKLILSARNLENLQ
                     KVKANCRSSNVEILPLDLSDFNLASKKVEQAISFFGKIDILINNGGISQRSLLVETAF
                     EVDKKLIEVDYLGTVALTKALLPHFINNHNGTFVTITSLMGKFGSPYRSGYCGAKHAL
                     HGFFDVLRMEHQKDNIQVTLICPGFIQTNVAKNALTADGSKQNTDDEATQNGMPVAVF
                     AQKLIRVVEKEQFEATIGGKETIGVLLKRFFPTFLHYFVLRSKVR"
     gene            complement(1142..1906)
                     /locus_tag="P5335_11750"
     CDS             complement(1142..1906)
                     /locus_tag="P5335_11750"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HRZ75599.1"
                     /translation="MKNIFKIPSFLLFIFCFSSFDTFGQEATIDTAKHVRKNIIRFNL
                     TSPMLFSKDYLVFGYERMLKENQSVSLNVGRFAMDKFGGNVSEELELKKNSKDKGLNL
                     SLDYRFYLGSVNRYNGPRGVYIGPYYSYNSFSRENTWTLNTDSFQGSVLTDLSFNINT
                     LGIQLGYQFIFWDRMALDLILIGPGVGFYGAKVKLDTDLSAEDEALFFDKLNEYLADK
                     IPGYDKVIGEGELSKNGSFDVTSLGFRYMIHLGYKF"
     gene            2170..>2418
                     /locus_tag="P5335_11755"
     CDS             2170..>2418
                     /locus_tag="P5335_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75600.1"
                     /translation="MLDAFLWGLLATSSLVLGGLIISWFSLSKKALGIIMAFGAGTLI
                     SAVAYELIYEAVKLGRGSFYPGIGFFTGAFTFYFSDLLI"
BASE COUNT          826 a          411 c          348 g          833 t
ORIGIN      
        1 aaaatttccg tttttaccgg aaatggtata atcgataacc gtagaatctt ttttggaagt
       61 taaataaacc gtagctgatt caaggggtag ttttgtgtta tcatctataa tcttaccatt
      121 tatagaaaca aaattttggc ttatagcaaa tgtgaaacaa aaaaacaggg taagtgtgaa
      181 aaaacattta gacatacaat gaaaattaac tgtgagaaaa ttctataaca gcgaattaac
      241 aattttagta tgaattgtaa aaatttctta cattaatttt aacatttaac gcactttact
      301 tcgcaaaaca aaatagtgca aaaaggttgg aaaaaatcgt tttaataaaa cacctatcgt
      361 ttctttacct ccaatagtgg cttcaaactg ttctttttca accacccgga ttaatttttg
      421 agcaaaaaca gcaacgggca ttccgttttg agttgcctca tcatctgtat tttgttttga
      481 accatcagct gttaaggcgt ttttagctac attggtctga ataaatcccg gacaaattag
      541 cgttacttga atattatctt tttggtgttc cattcgcaaa acatcaaaaa aaccatgtaa
      601 agcatgtttt gctccacaat agcctgagcg atagggcgaa ccaaattttc ccatcagact
      661 cgtaatcgtg acaaatgttc cgttatggtt atttataaaa tgaggcaaca gtgctttcgt
      721 taaagcaacg gttcctaaat aatctacttc aataagtttt ttatcaactt caaacgcagt
      781 ttccactaaa agagaacgtt gacttattcc cccgttattg attaatatat ctattttacc
      841 aaagaaagaa attgcttgtt caaccttttt tgaggctaga ttaaaatctg acaaatctag
      901 tggaaggatt tcaacgtttg aagaacgaca atttgctttc accttttgaa gattttccag
      961 atttctggca gaaagaatga gtttacagtt tttttgagcc aacgcatacg ctaacccttc
     1021 tcctattcca ctggaggctc cggtaatcca aacgatttta ttttctattg ttaccatatt
     1081 aaaataaaaa aagttccttg aatataaatg caaggaacta agttagtgtt ttaaaagtta
     1141 actaaaactt atagcccaag tgaatcatgt atcggaatcc aagactcgtc acgtcgaaag
     1201 aaccattttt acttaattca ccttcgccaa tcactttgtc ataaccgggt attttatcgg
     1261 caaggtattc atttaattta tcaaaaaaca aggcttcatc ttcagcagat aagtctgtgt
     1321 ctaatttaac ttttgcaccg taaaaaccta cccccgggcc aattagaatt aaatccaagg
     1381 ccattctatc ccaaaaaata aattgataac ccaattgaat accaagcgta ttgatattga
     1441 atgacaaatc agtaagtaca ctcccttgaa aagaatctgt atttaaagtc catgtatttt
     1501 cacgagaaaa actattgtaa gagtaatatg ggccaatata aactcctcta ggaccgttgt
     1561 atctattaac acttcctaaa taaaaacgat aatccaagga tagatttaac cctttatctt
     1621 tgctgttttt tttcaattct agttcctctg aaacattacc tccgaattta tccatggcaa
     1681 atcttcccac attcaatgag acagattgat tttctttaag catacgttca tatccaaaaa
     1741 ctaaataatc tttagaaaac aacataggac tggttaaatt gaatctaata atgttttttc
     1801 taacatgttt ggcagtatct atcgttgctt cctgaccaaa ggtgtcaaaa gaagaaaaac
     1861 aaaaaatgaa caataaaaaa ctcggaattt tgaaaatatt tttcattttt ataaaataaa
     1921 atttaacaat acgttttaaa taataggaaa tattctatcc aatttttaat tattagttac
     1981 acaaagttag aaaaatgtta tctcattttt aacattttaa ctgcctattt cttaactatt
     2041 tttagtcatt aaacattaat acttatttac attaaaaagt aatcattttt taaatcaatt
     2101 ttatccaatt tagtagagtc aaaacaaaaa attactactt ttatatacta atccgtaatt
     2161 atactaatta tgttagatgc ttttttgtgg ggtttgttag caacttcttc tttagtatta
     2221 gggggattga ttatctcatg gttttcatta agcaaaaaag ccttgggaat cattatggct
     2281 tttggtgccg gcactttaat ttctgcggtg gcctatgaac tcatctatga agcagttaaa
     2341 ttaggtagag gatctttcta cccgggaatt ggttttttta caggtgcttt taccttctat
     2401 ttcagtgatt tattaatt
//
LOCUS       DAOTCW010000232         2383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_38092_length_2383_cov_41.254296, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000232 DAOTCW010000000
VERSION     DAOTCW010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38092_length_2383_cov_41.254296"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="P5335_11760"
     CDS             complement(<1..671)
                     /locus_tag="P5335_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRZ75601.1"
                     /translation="MKKFIIPILTLSLSLLISCGSDRKETKTLVAIPVKVSGTVENTN
                     SPFVTASGKIEAENSANVSTRMMGYVTKVHVKVGQKVGAGQLLVSINNSDLQAKKAQV
                     DASILQATAGYNNAKKDYDRFIALFNQQSASQKELDDMTARFEMAKAGLEGVKQMRNE
                     VMAQFNYANITAPFSGTVTNSFVKEGDMANPGMPLVSVEGVSRLQVTAMVSESDISNI
                     KNGMPV"
     gene            complement(677..1990)
                     /locus_tag="P5335_11765"
     CDS             complement(677..1990)
                     /locus_tag="P5335_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRZ75602.1"
                     /translation="MKRIKEIFITGSLLVAFSVFGQDTIQISKSEIVQKMTDQNLQLK
                     IANKSVESARSDYRQSNSLFLPNISASHTAISTTNPLMAFGSKLNQEILTASDFNPAL
                     LNDPSQTQNYATKIEVLQPILNADGLYERKAAKAKIEAFQLQTERTKEYLELEVNKAF
                     MQLQLAYKAVQVFEKADATAKSNLKLVENYFKQGMLQKTDLLAVQVRVNEIVNQLQYA
                     KSNVRNASDYLAFLLNEDTKGGIYKPTEVLENEISITSMSSGFTANRKDILAMEKSSE
                     AYKQMMQSGKMAFLPRLNAFGAYELYDTEIFQFGASGYTVGAQLSWNLFDGYKSIGKL
                     EKSKAEFQKSEAETEQYKKQSQLELNKASRQLTDAENKVSLTKLAFEQTQESYRIRQN
                     RFTQGLEKTTDLLTDETQLAQKELEFLQSVFEYNFTKQYIQFLTK"
     gene            complement(2152..2280)
                     /locus_tag="P5335_11770"
     CDS             complement(2152..2280)
                     /locus_tag="P5335_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75603.1"
                     /translation="MQRISNSQKRKMQNPILQFFKFIYLNIRILKIVAGGHGGTRA"
BASE COUNT          716 a          430 c          362 g          875 t
ORIGIN      
        1 acaggcattc cgtttttgat atttgaaata tcactttcag aaaccattgc agttacttga
       61 agtcttgaaa cgccctcaac actgactaat ggcattccgg gattcgccat atcgccttct
      121 tttacaaaag aattggttac tgttccgcta aaaggtgctg tgatattggc ataattaaat
      181 tgggccatta cttcgtttcg catttgtttc actccttcta atccggcttt ggccatttca
      241 aaacgagccg tcatatcatc taactctttt tgtgaagcac tttgttgatt aaataaagct
      301 ataaaacgat cgtaatcttt tttggcatta ttgtatccgg cagttgcttg taaaatagaa
      361 gcatcaacct gggctttctt cgcttgcaaa tctgaattgt tgatactcac taataattgt
      421 ccggcaccta ctttttgtcc tacttttaca tggactttcg ttacatatcc catcattcgg
      481 gtactcacgt tagcactatt ttctgcttca attttaccac tggcagttac aaacggactg
      541 ttcgtatttt ccactgttcc actcacttta accggaatgg ctactaatgt tttggtttct
      601 tttctatcac tcccacaaga aataagtaaa gaaagtgaaa gtgtcaatat tggtatgata
      661 aactttttca taggttttat ttagttaaaa attggatgta ttgtttagta aaattatatt
      721 cgaaaacgga ttgcaagaat tctaattctt tttgagctaa ttgagtttca tcagttaata
      781 aatctgtagt tttctctaat ccttgcgtaa atctgttttg acgaattctg tagctttcct
      841 gagtctgttc aaaggccaac ttagttaaac tcactttgtt ttcagcatct gttaattgac
      901 gagaagcttt gttaagttct aactgacttt gttttttata ttgttctgtt tccgcttctg
      961 atttttgaaa ttccgctttt gatttttcaa gttttccaat ggatttatag ccatcaaata
     1021 agttccaaga taattgtgcc ccaacagtgt atccgcttgc tccaaactga aaaatctccg
     1081 tatcatataa ttcataagcc ccaaaagcgt tcaaacgtgg taaaaaagcc attttacccg
     1141 attgcatcat ttgcttataa gcttccgatg atttttccat agcaagaatg tcttttctat
     1201 tcgctgtaaa tcctgacgac atacttgtta tagaaatttc attttccaaa acttcagtag
     1261 gtttataaat accaccttta gtatcttcat tcaataaaaa agctaaataa tccgatgcat
     1321 ttctaacatt acttttggca tactgcaatt ggtttacaat ttcattcact cgaacttgaa
     1381 cagctaataa atctgttttt tgaagcattc cttgtttaaa ataattttca accaatttta
     1441 aattagattt tgctgtagca tcagcctttt caaaaacctg aacagccttg taagctaact
     1501 gtaattgcat gaatgcttta ttcacttcaa gttccaaata ttctttggtt ctttccgttt
     1561 gcaattgaaa tgcttcaatt ttagcttttg cagcctttct ttcatataat ccatctgcat
     1621 ttagaattgg ttgcaacact tcaatcttag tggcataatt ctgcgtttga cttggatcat
     1681 ttaaaagagc cggattaaaa tcggaagccg ttaaaatttc ctgatttaat tttgaaccaa
     1741 aagccatcaa tggattagtg gttgaaatag ctgtatgaga ggcagaaatg ttgggcaaaa
     1801 agagagaatt agactgacga taatctgaac gggctgattc aaccgatttg tttgctattt
     1861 tgagttgtag gttttgatcg gtcatttttt gtacaatttc cgatttggaa atttgtatag
     1921 tatcctgacc aaatactgag aaggcaacta gtaaacttcc cgtaataaaa atttctttta
     1981 ttcttttcat aatttattta ctgaattatg atgcaaattt aaatcgcaaa aaaagcctag
     2041 gaagtaacaa atgttacagt gtgaatgatg catcaaaaaa aacaagcatt tcttttcaaa
     2101 aatgcttgtt tcaacaatca ataacaatca tcaaacctcc ataagatgat attaggctcg
     2161 ggtgcctccg tgaccacccg ccacaatttt taaaattcga atatttaaat aaatgaattt
     2221 aaaaaactgt aaaataggat tctgcatttt tcgtttttgc gaattggaaa ttctttgcat
     2281 atttatattt tttaaactga attattttca gctattacta attattttac tcaggaatta
     2341 accaccaaaa cggtttcatt tttgctttat ataaaaaagc ata
//
LOCUS       DAOTCW010000233         2368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_38459_length_2368_cov_26.332901, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000233 DAOTCW010000000
VERSION     DAOTCW010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38459_length_2368_cov_26.332901"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..2291
                     /locus_tag="P5335_11775"
     CDS             159..2291
                     /locus_tag="P5335_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRZ75604.1"
                     /translation="MNKKFILFFSLFLAISVNAQLKKITLDESVLQQGRKFGADRLTG
                     FQWVPNSDKYVYYTDVWTKMMSASSKANKPTELVTLTDMNLALGTNLKNFFGLEWIDT
                     NTILVSEKGKYYTYSLTSKSGKITQVTPENAENQTFDLSKTNLAFTEENNLYFLDKNN
                     KKIAVTNETNKEIVSGQSISRNEFGIKNGIFWSPKSSYLAFYQKDETEVADYPLLDIN
                     ETPGKLISIKYPMIGQKSEKPRVGIFNLATGKTVFINPKNNPDDYLTNLSWSPDEKYV
                     LIAELNRGQNDMNLNVYDANSGEFIRTIFNEKNAKWVEPEHDAFFPNAKSNNFIWLSE
                     KSGFQNLYYYTIEGKLIKQLTSNKFPVREIMRANPAGTEIYFKATGENPTNMLVYKVD
                     LKGKQTLMTKNEGVHNVVISSDGNWFFDEFSNHSTPSKSLLYDKKMNATTLLESKNKY
                     DGYEMGTAEIRTIKAADGTTDLYTRLIKPSHFDPNKKYPVMVYVYGGPHAQMITNSYL
                     DGANLWMYWMAEQGYLVFTVDNRGSDNRGFEFESVIHGRCGFNEIEDQIKGIDYLKSL
                     PYVDSNRLAVHGWSYGGFMTASMMLRKPDVFKVGVAGGPVVDWKWYEVMYGERYMDTP
                     AENQKGFEEASVLNYVKELKGKLLLIHGTNDDVVVEQHNLSLVKKFVEAGKQVDYFPY
                     PMHKHNVNGKDRVHLMAKVLNYIIDNNK"
BASE COUNT          886 a          325 c          414 g          743 t
ORIGIN      
        1 ccaacgctat tggtattgtt caggaatatt tagataagga aaatgaagag aacttgagtt
       61 aaaatcaata aatcattcaa atttataaag ggtactattt caattcaaat gaatagtatc
      121 cttttttata tctttacaaa aaacaaacat actatattat gaataaaaaa tttatactct
      181 tctttagttt atttttagcg atttccgtta atgctcaatt aaaaaaaatc acattagatg
      241 aaagtgtatt acaacaaggt agaaaatttg gtgctgacag attaaccggt tttcaatggg
      301 ttccaaactc cgataaatat gtttattaca ccgatgtctg gactaaaatg atgtcagctt
      361 cttctaaagc taacaaacca actgaattgg ttactttaac agatatgaat ttagctttgg
      421 gaaccaattt aaaaaacttt tttggtttgg agtggattga tactaataca attttagttt
      481 cggaaaaggg aaaatattat acgtattctc taacatcaaa atcaggaaaa ataacgcaag
      541 taactccgga aaatgcagaa aatcaaactt ttgatttatc caaaaccaat ttagccttta
      601 cagaagaaaa taatttatat ttcttagata agaacaataa aaaaattgct gttacgaacg
      661 aaacaaataa agaaattgtt tcaggccaat ctatttccag aaacgaattt ggcattaaaa
      721 acggtatttt ttggtcgcca aaatcttcct atttagcatt ttatcaaaag gatgaaacag
      781 aagtcgctga ttatccttta ttagatatta atgaaacacc cggaaaatta atttctatta
      841 aatatcctat gattggtcag aaatctgaaa aaccaagagt tggaattttt aatttggcaa
      901 caggcaaaac tgtttttatc aacccaaaaa ataatcctga cgattattta accaatcttt
      961 cttggtcacc ggatgaaaaa tacgtcttga ttgcagaatt aaatcgaggt caaaatgaca
     1021 tgaatttgaa tgtttatgat gccaattcgg gtgaatttat tagaactatt tttaacgaaa
     1081 agaatgctaa atgggttgaa ccggagcacg atgccttttt tccaaatgct aaatcaaaca
     1141 attttatttg gctcagtgaa aaaagtggtt ttcaaaattt atactattat actatagagg
     1201 gtaaattaat caaacaactt acttcaaata aatttccggt aagggaaatt atgcgagcaa
     1261 atccggcagg aacagagatc tattttaaag caacgggtga aaatccaaca aatatgttgg
     1321 tttataaagt agatttaaaa gggaaacaaa cgctaatgac taaaaacgaa ggtgttcaca
     1381 atgtggttat ttccagcgat ggaaattggt tttttgatga attctcgaat cattcaactc
     1441 catcaaaatc attgctgtat gataaaaaaa tgaacgctac gactttattg gaaagcaaaa
     1501 ataaatatga tggttatgaa atgggaaccg cagaaatcag aactattaaa gctgctgatg
     1561 gtacaaccga tttatatacc cgtttaataa aaccaagcca ttttgatcca aacaaaaaat
     1621 atcccgttat ggtttatgtt tatggaggtc cacatgctca aatgataaca aattcctatt
     1681 tagatggagc taatttgtgg atgtattgga tggcagaaca aggttattta gtttttacag
     1741 tagacaatcg cggatcggat aatcgtggtt ttgaatttga aagcgttatt catggccgat
     1801 gtggttttaa tgaaattgag gatcaaatca aaggaattga ctatctaaaa tctttacctt
     1861 atgttgacag caatcgatta gctgttcacg gttggagtta tggcggattt atgactgcct
     1921 ccatgatgtt aagaaaacct gatgtattta aagttggtgt tgccggcgga cctgttgttg
     1981 attggaaatg gtatgaagtg atgtatggtg aacgttatat ggatacacct gctgaaaatc
     2041 aaaaaggttt tgaagaagct agtgttttaa actatgtaaa agaattaaaa ggaaagctat
     2101 tattaattca cggaacgaat gatgatgtag tggttgaaca acataattta tctctagtca
     2161 aaaaatttgt ggaagcagga aaacaagtag attattttcc atatccaatg cacaaacaca
     2221 acgtaaatgg caaagacaga gtgcatttga tggctaaagt tttaaattat attattgata
     2281 ataataaata aagtgcttta gaaaaaagag ttgaaaaaac cgaaagaaaa atcactttcg
     2341 gtttttttag ttaagacaac actttata
//
LOCUS       DAOTCW010000234         2362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_38607_length_2362_cov_41.485046, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000234 DAOTCW010000000
VERSION     DAOTCW010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38607_length_2362_cov_41.485046"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="P5335_11780"
     CDS             complement(<1..391)
                     /locus_tag="P5335_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRZ75605.1"
                     /translation="MNYTTIPNTDLKISKICLGTMTFGEQNSESDAHQQLDYAISRGI
                     NFIDTAEMYPIAAREETLGSTERYIGSWLKARGKRDDLIIATKIAGPNRGMDYIRKPL
                     DFSKKSILEAVDLSLKNLQTDYIDLYQM"
     gene            complement(534..1499)
                     /locus_tag="P5335_11785"
     CDS             complement(534..1499)
                     /locus_tag="P5335_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRZ75606.1"
                     /translation="MIKKVSLLVLVAALATSCVSKKVYNELESKFAALKKEQRQLSDE
                     NHDLEKTKNKLEVDLKANQDELARIKAERDKLQNELATASTNLKTLTASYTALEKNSD
                     EALQANMQKNRELLAQLDAKEKALLAEQERLNKLQADLKERSQRVTDLENLISSKEES
                     MRKLKETLSKALNSFEGKGLTVEQKNGKVYVSMENKLLFQTGSWAVGTEGKRAVVEVG
                     KVLAQNPDISVLIEGHTDNDKILGSIGGGIENNWDLSTKRATAIVNILAENKGINKQN
                     LTAAGRGEFAPLMSNDSPEGKAKNRRIEIILTPKLDEISKMLNDL"
     gene            complement(1535..2296)
                     /locus_tag="P5335_11790"
     CDS             complement(1535..2296)
                     /locus_tag="P5335_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRZ75607.1"
                     /translation="MRIISYNVNGIRAAITKGFLEWLQQANPDVICLQEIKATEEQIP
                     TEEITKAGYPYQYYFPAQKKGYSGVAILSKVQPKNVVFGTGIASMDFEGRNLRADFED
                     FSVMSLYLPSGTNIERLDHKFNYMDEFQQYINALKVEVPNLIIGGDYNICHQAIDIHD
                     PIRNATVSGFLPEERRWLDGFMKSGFIDSFRHFNKEPHQYSWWSYRANARNNNKGWRI
                     DYHLVSEPLKNRLKRALILPEAKHSDHCPILVEIE"
BASE COUNT          680 a          446 c          358 g          878 t
ORIGIN      
        1 gcatttggta taaatcaatg taatccgttt gcagattttt caaactcaaa tcaactgctt
       61 ctaaaatact tttctttgaa aaatccaatg gttttcgaat ataatccatt ccgcgattcg
      121 gtccggctat tttagtggcg ataatcaaat catctctctt acctcttgct tttaaccaag
      181 agcctatgta acgttccgtg cttcctaatg tttcttcacg agctgcaata ggatacattt
      241 ctgccgtatc tataaaatta attccgcggg aaatagcata atctaattgt tgatgagcat
      301 cactttctga attttgttct ccgaaagtca ttgttcctaa acaaattttg ctgattttta
      361 agtccgtgtt tgggatggtg gtgtagttca tttttgaatg gctgaagctc tgagtggctg
      421 agactctaag ttttctgttt tagaaataag cattatacat ttttattgga gtggttaaaa
      481 tactgagaag tttaaaatca attgctcagt atctcaatta ctcagtaact agtttacaaa
      541 tcattcagca ttttagaaat ctcgtctaat tttggtgtta aaataatttc aattcgtcgg
      601 ttttttgctt ttccttccgg agagtcattt gacattaatg gagcaaattc ccctcttccg
      661 gctgccgtta aattttgttt attgattcct ttattttcag ctaaaatatt cacaatagca
      721 gtagctcttt ttgttgagag atcccagtta ttttctattc ctccaccaat tgaacctaat
      781 attttatcat tgtcggtatg cccttcaatt aaaacagaaa tatcagggtt ttgagctaat
      841 actttaccaa cttctacaac tgctcttttt ccttcagttc caacagccca acttccggtt
      901 tgaaataata atttattttc catggaaaca taaacttttc cgtttttttg ttccacagta
      961 agccccttcc cttcaaaact attcaatgct tttgaaagtg tttcttttaa ttttcgcatg
     1021 ctttcttctt tcgatgaaat taggttttct aaatcagtta cacgttgcga acgttccttt
     1081 aaatcagctt gtaacttgtt taagcgttct tgttctgcta acaatgcttt ttcttttgcg
     1141 tctaattgtg ccaataattc tctgtttttc tgcatgttag cctgcaaagc ttcatcagaa
     1201 tttttctcca aggcggtata agaagcggtc aatgttttta aattagtaga tgctgtagct
     1261 aattcatttt gcaatttgtc tctttccgct tttattcgag ccaattcatc ctgattggct
     1321 tttaaatcaa cttctaattt atttttagtc ttttctaaat catggttttc atcagaaagt
     1381 tgacgctgct cttttttcaa agctgcaaat ttgctctcta attcattata cacttttttg
     1441 gacacgcaag aagtagccaa ggcggcaact aaaactagta atgaaacttt tttaatcatt
     1501 atttaaagta tttgaggttc tatattgata taacttactc tatttccact aaaatcggac
     1561 aatggtcaga atgttttgct tctggtaaaa ttaatgcccg tttcaatcta ttctttaagg
     1621 gttcgctcac tagatgataa tcaattcgcc aacctttatt gttatttctg gcattggctc
     1681 ggtaactcca ccaggaatac tgatgcggtt ctttattaaa atgacgaaaa ctgtctataa
     1741 aaccactttt cataaaacca tctaaccaac gtcgttcttc cggcaaaaaa ccggaaactg
     1801 tagcattccg tatcgggtcg tggatgtcaa ttgcttgatg acaaatgtta taatcaccac
     1861 caataatcaa attaggaact tcaactttca atgcgttaat atactgttga aattcatcca
     1921 tataattaaa tttatgatct aaccgttcaa tattagttcc ggagggtaag tataaactca
     1981 ttacagagaa atcttcgaaa tcagctctta gatttcgccc ttcaaaatcc attgaagcaa
     2041 ttcctgttcc gaaaactaca ttttttggtt gaactttaga aagaattgct accccactat
     2101 atcccttttt ttgagccgga aaataatatt gataaggata acctgctttg gtaatttcct
     2161 cagtaggaat ttgttcttca gtagctttaa tttcctgtaa acaaattaca tcaggatttg
     2221 cctgttgaag ccactctaaa aaaccttttg taatggctgc tcgaatacca tttacattat
     2281 aagaaataat acgcatttag aatttttagt aaagtttttc aaaaatagat aaaaaccttg
     2341 agtatcaaaa cagactggtg ga
//
LOCUS       DAOTCW010000235         2303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_40127_length_2303_cov_51.391014, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000235 DAOTCW010000000
VERSION     DAOTCW010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40127_length_2303_cov_51.391014"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2246)
                     /locus_tag="P5335_11795"
     CDS             complement(<1..2246)
                     /locus_tag="P5335_11795"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRZ75608.1"
                     /translation="MKRLKKIVVVIFILFVILAISVVSYGYFLTPKYDGELALNSIQK
                     ETTVYFDEYGIPHIYAASQKDAMTTLGYVHAQDRLWQMELMRRIAPGRLSELFGSVML
                     KNDKFFTGLGIDEDSEKAIAQLDKNSESYQLTLAYLDGVNQFLEEGPTPIEFQLVGVT
                     KEKFTIKDVYNIFGYMSFSFAMAQKTDPLLTDIRDEFGMDYLKDFGIDGSLAHTQLKS
                     FKGKSEAYVEISKSVTSLLENSPIPSFIGSNSWVVGPQKTKSGKVLFANDPHIGFSQP
                     ATWYEAHIVTPGYEMYGYYLAGTPFPLLGHNRQYAYGLTMFENDDMDLFLETNNPENE
                     NEYQSPEGYKNYQISEKTIKVKDSTDVVLKIKKSHHGPLMNGLVDGLKDDKPVSMWWT
                     YLHHKNQILDAVYALSHAKNVDDFHQSIELIAAPGLNIMYGDAKGNIAWITSGKLYKM
                     PEQVNANFILDGSNGNDEQKDYYDFSENPYAINPDWKYVYSSNNQVEPINGYLYPGYY
                     LPEDRAKRIVQLLEPKNDWTKEDFMNMITDETSSVAPTVAKNMINSISQEVLSENEKA
                     AISILLKWKGSNALSDVAPTIYNKWMYLYLKNTFEDELGKDRFSVLLNTHIMKQMIAF
                     QTSNESSPWWDDVSTKDKSETRTDILTKSFQEAILQLEKQLGNDLNAWTWNKVHSVEH
                     KHPLGEVAALRSYFNVGPFEINGSNEVINNLMFTFTEAGNHQVKAGPSTRRIIDFSDI
                     ENSMSILPT"
BASE COUNT          712 a          427 c          382 g          782 t
ORIGIN      
        1 gttggtagaa tgctcatact gttttcaata tctgaaaaat cgataatgcg acgagtcgaa
       61 ggaccagctt ttacttggtg atttcctgct tcagtaaacg taaacattaa attattgatg
      121 acttcattgg aaccattgat ttcaaacgga cccacattga aataacttcg taacgcagct
      181 acttcaccta aaggatgttt gtgttctact gaatgaactt tattccatgt ccacgcattc
      241 aaatcgtttc ctaattgttt ttcaagttgc agaatagctt cttggaagga ttttgtcaaa
      301 atatccgttc gggtttctga cttatccttt gtagaaacat catcccacca aggtgacgat
      361 tcattagaag tttgaaaagc aatcatttgt ttcatgatgt gtgtattcaa caaaacagaa
      421 aatcggtctt ttcctaactc atcttcaaaa gtgtttttta aatacaaata catccattta
      481 ttgtaaatag tgggtgccac atcactcaat gcattggaac ctttccattt taataaaata
      541 gaaatggctg ctttttcatt ttcagacaag acttcttgac tgatggaatt aatcatattt
      601 ttggcaactg ttggagccac ggaagaagtt tcatccgtaa tcatgttcat aaaatcctct
      661 tttgtccaat cgtttttggg ttcgagtaat tgaacgattc gtttcgctcg atcttccggt
      721 aaataataac ccgggtataa atagccattt ataggttcca cttgattatt ggaagaatag
      781 acatacttcc aatccggatt aatagcataa ggattttcag aaaaatcata gtagtctttt
      841 tgttcgtcat ttccgtttga accatcaaga ataaaattgg cgttgacttg ttcaggcatt
      901 ttatacaatt taccggaagt aatccaagcg atgtttcctt tggcatcgcc atacataata
      961 tttaatccgg gagcggcaat caattcgata ctttgatgaa aatcgtcaac atttttcgca
     1021 tgcgaaaggg cataaaccgc atctaaaatt tgattcttat ggtgcaaata ggtccaccac
     1081 attgatacgg gtttgtcgtc tttcaaacca tccaccaaac cattcattaa aggtccgtga
     1141 tgcgattttt taattttcaa aacaacatcc gtactgtctt ttactttgat tgttttttca
     1201 gaaatttgat agtttttata tccttccgga gattggtatt cgttttcatt ttcgggatta
     1261 ttggtttcca aaaacaaatc catatcgtca ttttcaaaca tcgttaaacc ataggcatat
     1321 tggcgattgt gacccaaaag tggaaaaggc gttccggcta aataatagcc atacatttca
     1381 taaccgggtg tcacgatgtg agcttcatac caagtagccg gttgtgaaaa tcctatgtgt
     1441 ggatcgtttg caaataatac tttgccgctt tttgtttttt gtggtccaac tacccaactg
     1501 ttacttccta tgaatgatgg aatcggtgaa ttttctaata aggaagtgac tgattttgaa
     1561 atttcaacat atgcttctga ttttccttta aaacttttta gttgggtatg agccaacgag
     1621 ccatcgatgc caaaatcttt taaataatcc attccgaatt catcgcgaat atcggttaac
     1681 agcggatctg ttttttgagc cattgcaaaa ctaaaagaca tatacccaaa aatattatac
     1741 acatctttga tggtgaattt ttccttagta acgccaacca attgaaactc aattggcgtt
     1801 ggaccttctt ctaaaaactg attaactccg tccaaataag ctaatgttaa ttgataactt
     1861 tcgctatttt tatccaattg agcaattgct ttttctgaat cttcatcaat gcctaaaccg
     1921 gtaaaaaatt tgtcattttt aagcataact gaaccaaaga gttcggacaa tcttcccgga
     1981 gcaattcttc gcatcagttc catttgccac aatcgatctt gagcatgtac ataacctaat
     2041 gtggtcattg catctttttg cgaagcagca taaatgtgtg gaattccgta ttcatcaaaa
     2101 taaacggtgg tttctttttg gatagaattt aatgctaatt cgccatcgta tttaggggtt
     2161 aaaaaataac cgtaactcac aacactaata gctaaaatga caaaaagaat aaatataacg
     2221 acaacgattt ttttgagtct tttcatgaca attgaatttg atttaaagtt acaaaaaacc
     2281 attaagaaat taagtaacat taa
//
LOCUS       DAOTCW010000236         2302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_40158_length_2302_cov_40.603916, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000236 DAOTCW010000000
VERSION     DAOTCW010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40158_length_2302_cov_40.603916"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..841)
                     /locus_tag="P5335_11800"
     CDS             complement(2..841)
                     /locus_tag="P5335_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRZ75609.1"
                     /translation="MKRILFPTDFSEASNNAFIYALKLADAIKAEVITLHAYELPQLH
                     IGGLPSTLKEVYDSIELENFENFKDQIPVLRDVAERYHLSHVKISNILKHGDLVWAIK
                     KVVKEEHIDYVVMGTKGASGLKETFLGSNTGSVITEVEAIVIGVPEDSDFTGINNIVF
                     TTRFREKDLKALKKIIELANLFEAKIHCLYIKTKKSDVKEVVIEDWKFLLKGENVVFH
                     IYESEDVSTSILNFIDSYNVDMLAMLNYKRGFFEELFKQSMTQKLSYHVKVPILALHE
                     NTI"
     gene            1013..>2302
                     /gene="mnmE"
                     /locus_tag="P5335_11805"
     CDS             1013..>2302
                     /gene="mnmE"
                     /locus_tag="P5335_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRZ75610.1"
                     /translation="MIPNETIVALATPSGAGAIAVIRISGKNSIQIASTVFQSVSGKD
                     ITKQKTHTLHLGYVADGPKIYDQVLLSIFKNPNSYTGEDVVEISCHGSVFIQQQIIQL
                     LLRKGCKMAQPGEFTLRAFLNGKLDLSQAEAVADLISSDNEASHQIAMQQMRGGFSNE
                     IAKLREELLNFASLIELELDFAEEDVEFADRTQFHDLLNRIEFVLKRLIDSFAVGNVI
                     KNGIPIAIVGEPNVGKSTLLNALLNEERAIVSEIAGTTRDTIEDELVIEGIGFRFIDT
                     AGIRETNDVVESIGIKKTFEKIEQAQVVVFLVDSSQLSLFGSQLSVDQLKMEIEQIRN
                     KYPHKTILTIINKKDLISSEMLHMLETQLTTENKQLQTLFISAKNKEGIDELKQTLLS
                     FVNTGALRNNETIITNTRHYDSLLKALEEIQKVKWGLE"
BASE COUNT          795 a          405 c          337 g          765 t
ORIGIN      
        1 atcaaattgt attttcgtgt aaagctaaaa taggcacttt cacatgatat gacaattttt
       61 gagtcatact ttgtttgaat aattcttcaa agaaccctct tttgtaatta agcattgcta
      121 acatatcaac attataagaa tctataaaat tcaaaataga agtactaaca tcttcacttt
      181 cataaatatg gaagactaca ttttctcctt ttaataaaaa cttccaatct tcaattacca
      241 cttcttttac atccgatttt ttagttttga tatataaaca atgaatttta gcctcaaaca
      301 aattggctaa ttctataatt tttttcaatg cttttaaatc tttctcacgg aatcttgtgg
      361 taaaaactat attattgatt cctgtaaaat ctgaatcttc cggaacacca ataacgatgg
      421 cttctacttc agtaataacg cttccggtat tagaacccaa aaacgtttct ttcaaaccac
      481 ttgccccttt agttcccatt accacataat caatgtgttc ctcttttaca acttttttaa
      541 tagcccaaac taaatcaccg tgttttaata tattactgat ttttacatga ctcaagtgat
      601 atctttctgc aacatctctc aaaaccggaa tttgatcttt gaaattttca aaattttcaa
      661 gttcaatact gtcataaacc tctttcaatg tacttggcag tccaccgata tgtaattgag
      721 gtaactcata agcgtgaagt gtaatcactt ccgccttaat tgcatctgcc aattttaaag
      781 cataaatgaa cgcattatta gatgcttctg aaaaatcggt tggaaaaaga atacgtttca
      841 ttagttctga tatttaaatt atataaaatt aacttacgta aatttagcaa aattttcacg
      901 tagattgtat gataaatgtc attaataaca actatttaac aatgtccaaa agacttatat
      961 ttccgtcgtg tttcattaat tattgctaat tttacaacaa aatccgattg ctatgattcc
     1021 aaatgaaacg attgttgctt tagctacacc ctccggtgca ggagctatcg ctgtaattag
     1081 aatttccggt aaaaattcaa ttcaaattgc ttccaccgtt tttcaatccg tttccggaaa
     1141 agacatcact aaacaaaaaa cacataccct tcatttagga tatgttgcag atggtccaaa
     1201 aatatatgac caagttttgc tttcaatttt taaaaatcca aattcttata ccggagaaga
     1261 tgtggtagaa atttcgtgtc atggttcagt ttttatacaa caacaaatta ttcaactttt
     1321 acttcgtaaa ggatgtaaaa tggctcagcc gggtgaattt actttacggg cttttttaaa
     1381 tggtaaactg gatttatctc aggcagaagc cgttgccgat ttaatttctt ctgataatga
     1441 agcctcacac caaatagcca tgcagcaaat gcgaggtggc tttagtaatg aaattgccaa
     1501 attaagagaa gaactattaa attttgcttc actgattgaa ttagaattag attttgcgga
     1561 agaagacgtt gaatttgctg acagaacaca atttcatgat ttattaaata gaattgaatt
     1621 tgttttaaaa cgattaatcg attcattcgc cgttggaaat gtcatcaaaa atggaatacc
     1681 aattgccatt gtgggcgaac ccaatgtagg taaatctact ttgttaaatg ctttattaaa
     1741 cgaagaaaga gctatagtat cagaaattgc tggaacaacc cgtgatacca ttgaagatga
     1801 attagtcatc gaaggtatag gattccgttt tattgatact gccggaataa gagaaaccaa
     1861 tgatgttgtg gaaagtattg gcatcaaaaa aacgtttgaa aaaattgaac aggctcaggt
     1921 cgttgttttt ttagttgata gttctcagtt gtcccttttc ggttctcagt tgtcagttga
     1981 ccaattgaaa atggagattg aacaaataag aaataaatat ccccataaaa caattttaac
     2041 gattatcaat aaaaaagact tgatttcatc tgaaatgctc catatgttag aaacacaact
     2101 aacaaccgaa aacaaacagc tgcaaactct ttttatctct gctaaaaaca aagaaggcat
     2161 cgatgaactc aagcaaaccc tactctcttt tgtcaacacc ggtgctctac gcaataatga
     2221 aaccatcatt accaatacac gccattatga ttctctttta aaagctttag aagaaattca
     2281 aaaagtgaaa tggggattag aa
//
LOCUS       DAOTCW010000237         2278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_40820_length_2278_cov_40.215924, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000237 DAOTCW010000000
VERSION     DAOTCW010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40820_length_2278_cov_40.215924"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="P5335_11810"
     CDS             complement(<1..941)
                     /locus_tag="P5335_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRZ75611.1"
                     /translation="MKKILFFSFILISHLGFAQELPPIVKYPPSSYNAGNQNWMISQD
                     KNNFVYFANNEGLLEFNGSDWTIYPSPNETIMRSVKVIDEKIYTGCYMEFGFWKRLSN
                     GQLKYTSLSSKVKSKILDDEQFWSILHFDHWVIFQSLNQLFIYDTKTSTFEVITPKSG
                     IFKAYITSKSIFYQTVSEGLFEIENGKGKLFSKNPFFIQNRIISIFKQDNSFLFLTQY
                     NGFYEYKNEIIQKWVTDSDPEINSSSIYCAQKNSDNSIVFGTVSNGIFIIDSNGKTKY
                     HITQNKGIGNNTVLSLFEDTEKNIWIGLDNGINCINLS"
     gene            1179..1448
                     /locus_tag="P5335_11815"
     CDS             1179..1448
                     /locus_tag="P5335_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRZ75612.1"
                     /translation="MKTTIKVQNLKCEGCANTISKKLHTIESVSIVEVDVEKSLVEVS
                     YENEGIIETIKSTLNSLGYPEEGESNTFGNKAKSYISCAIGKIDL"
     gene            1498..1923
                     /locus_tag="P5335_11820"
     CDS             1498..1923
                     /locus_tag="P5335_11820"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRZ75613.1"
                     /translation="MKKISIFFIALLSLGLTTSSCSDDENTSIVGKWIFSQEGFAVEG
                     GQEVLEPYQHTTGCNQDYVEFTAEGVVKNENYFNEGAGCESDLSMGTYLKSDNNLTLT
                     IDGETVNATIITLNSSTLKIKVTVVEGAETVSYITVFSR"
     gene            complement(1968..>2278)
                     /locus_tag="P5335_11825"
     CDS             complement(1968..>2278)
                     /locus_tag="P5335_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HRZ75614.1"
                     /translation="LYLVGFLYFLWEKLGGMKIPVIIYALVISTMLLVAIKGYFTWNS
                     KSGKLLLIGAVFFVLSDSILAINKFYVPIYLSSFWIMSTYITAQFLIVKGVLNLNSKQ
                     "
BASE COUNT          862 a          302 c          338 g          776 t
ORIGIN      
        1 gagagattta tgcaattgat tccgttatca agcccaatcc atatattttt ttcagtatct
       61 tcaaataaag ataaaaccgt attgtttcca attcctttat tttgagtgat atggtatttt
      121 gttttaccat tggaatcaat gatgaaaatt ccatttgaaa cagtaccaaa aacaatacta
      181 ttatctgagt ttttctgggc acaatatatg ctgctggaat taatttctgg atcagaatcg
      241 gttacccatt tttgaattat ttcattttta tattcataaa agccattgta ttgtgtcaga
      301 aaaaggaaag aattgtcctg tttgaaaatt gaaattattc tattttgaat aaaaaaagga
      361 tttttagaaa acaatttgcc tttaccattt tctatttcaa atagtccttc agaaacagtt
      421 tgataaaaaa tggatttgga agtaatatat gctttaaaaa tgccactttt aggagttata
      481 acttcaaatg tagaagtttt tgtatcataa atgaataact gatttaaaga ctggaaaata
      541 acccaatgat cgaaatgtaa gatggaccaa aactgttcat catctagtat ttttgatttg
      601 acttttgaac ttaaagaagt atattttaat tgtccgtttg ataatctttt ccaaaaacca
      661 aattccatat aacagccggt atagattttt tcatctatca cttttaccga tctcataatc
      721 gtttcattag gcgatggata aatagtccag tcagaaccgt taaattccaa caagccttca
      781 ttatttgcaa aataaacaaa attgttttta tcttgactaa tcatccaatt ttggttccct
      841 gcgttgtagc tactcggtgg atattttacg atgggaggga gttcttgagc aaaacccaaa
      901 tgcgaaatta gtataaaact aaaaaatagt atttttttca agagttgcaa ttttttttaa
      961 aatttaagct aaagatagtt tttaaaaata aagaaagcaa atgtacattt taaaaaaaaa
     1021 gtcaactttg aaacatactt aaccaattga aaatgttgta gttttataat caattagttt
     1081 ttttatgtgc tttcaaatga aaaaaaatca gaaagttata atcttttttt agacttattt
     1141 atcatcaaaa tgagtaaatt tgcataaaat aataagttat gaaaacaacg ataaaagttc
     1201 aaaatttgaa atgtgaagga tgtgccaata caatttcaaa aaaattacat accattgaat
     1261 ctgtttctat tgtagaagtg gatgttgaaa aaagtttggt agaagtaagt tatgaaaatg
     1321 aaggaatcat agagactatt aaatcaactt taaatagttt aggttatccg gaagaaggag
     1381 aatcaaacac ttttggcaac aaagcaaaat cctatataag ttgtgcaata ggaaaaattg
     1441 atttataatt ataaatttta tagtaatttt acatcattaa tttaaaaaca cattaaaatg
     1501 aaaaaaatat caattttctt tattgcctta cttagtttag gattaacaac cagttcttgt
     1561 agtgacgatg aaaacacatc gattgttggt aaatggattt tctctcaaga aggtttcgcc
     1621 gtagaaggag gccaagaagt tttagaaccg tatcaacata cgaccggttg caaccaagat
     1681 tatgttgagt ttactgccga aggcgtagtt aaaaatgaaa attattttaa tgaaggtgcc
     1741 ggttgtgaat ctgatttgtc aatgggaact tatctaaaaa gtgataacaa cttgacttta
     1801 acaatagatg gagaaactgt taatgcaact attataacct taaacagttc aactttaaaa
     1861 attaaagtca ctgtggttga aggagcagaa acagtcagtt atataactgt attcagtaga
     1921 taataaaatt tcaatttgtt taaagaggct caaattttga gcctttttta ttgctttgaa
     1981 tttaaattca aaaccccttt tacgattaga aactgagcag taatataggt actcatgatc
     2041 caaaaagaac tcaaataaat aggcacgtaa aatttattaa tcgctaaaat actgtcagaa
     2101 agaacaaaaa atacagctcc aattaaaagt aattttccgg attttgaatt ccaagtaaaa
     2161 tatcctttta ttgctactaa tagcattgta gaaataacta aagcatatat aattaccgga
     2221 attttcatgc ctcctaattt ttcccaaaga aaatatagga atccaactaa ataaagta
//
LOCUS       DAOTCW010000238         2272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_40993_length_2272_cov_39.174560, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000238 DAOTCW010000000
VERSION     DAOTCW010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40993_length_2272_cov_39.174560"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="P5335_11830"
     CDS             <1..138
                     /locus_tag="P5335_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRZ75615.1"
                     /translation="KEIHATHQEIAYDLHTSRVVISRLLKALENDNKIKLHRASIELL
                     K"
     gene            complement(153..>2272)
                     /locus_tag="P5335_11835"
     CDS             complement(153..>2272)
                     /locus_tag="P5335_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRZ75616.1"
                     /translation="IDQDENYLTYRNLSVTSLMLNEISNRIENQLIKIKGNNDVLSSS
                     QNKIDSLFISKELYISPKDTAARTIYFNQFMQLNGDYKEINSLLRNGLDSIHKLEILG
                     SKFKFNLQSDIIEINNLKKTELDALFSKKFNLFSKSQEKENFVSTFIYSLKKEILLLL
                     FYAFNHFDFFIIIFLAFLATAFYLKLLKKKYEKANLYDKFNYPVQILRHPFSSSIVIV
                     FTLFQFFLPQPPFALMAIFWTVIGISLTILFKKTTSVYYYTFWIGIFILNLLAIFDNT
                     ILLHSVSESWFLVFIASTSILFGLHTLKNRKKWDSELMNWIVVLMIILEVVGIVFMLL
                     GNYNFAKVIITIGLYNIFIAIMLLSTFHLTKDIGLYSEYLKDSDEEKKLNLKTIEYHK
                     ISKKYVFLFVLGWFILVARNTYWYQKIISPFELEFSKPRNIGDFNYTYNSVFIFFMVL
                     IISTFISKIVSFLASDKRQSLTNGKTNKIGSWLLLIRILIICIGIIIAFSSAGIPMNQ
                     FTIIISALSVGIGFGLQTLVNNLVSGIIIAFEKPVNLDDIIEIGGQTGQMKSIGLRSS
                     VVTTYDGADVIIPNGDLLNQHMTNWTMGSAKRRYEINIGVAYGTDLKLTKSLIEKVLS
                     EHALVLKNPAPMVWFTIFNDSSIDFTIKYWVPHFNYGNDVKSDLIIAIDEIFKANNIV
                     IPFPQQDIHIQTNLSKFDESEEV"
BASE COUNT          843 a          318 c          311 g          800 t
ORIGIN      
        1 aaagaaattc atgcaaccca tcaagaaatt gcttacgatt tgcacacttc cagagttgtt
       61 atttctcgat tattgaaagc gttggaaaat gacaataaaa ttaaattgca cagagcttct
      121 attgaactat tgaaataaaa cttttttaat tattaaactt cttcagattc atcaaattta
      181 ctcagatttg tttgaatatg tatatcttgt tgaggaaaag gaattacgat attatttgcc
      241 ttaaagattt cgtctattgc aataattaaa tcacttttca catcattacc ataattaaaa
      301 tgaggtaccc aatatttgat agtaaaatct attgaactat cattaaaaat agtaaaccat
      361 accatgggag ctgggttttt aagcactaaa gcgtgttcac ttaatacttt ttcaataagt
      421 gattttgtca attttaaatc ggttccataa gcaacaccaa tatttatttc ataacgtctt
      481 ttggcacttc ccattgtcca atttgtcatg tgttgattga gtaaatctcc atttggaata
      541 ataacatctg caccgtcgta ggtggtcaca acactacttc gtagaccaat cgatttcatt
      601 tgaccggttt gtcctccgat ttcgattata tcatccagat ttacgggttt ttcaaaagca
      661 attataattc cactgactag gttgtttacc aatgtttgca aaccgaaacc aataccaaca
      721 ctcaaggcac taatgataat tgtaaattga ttcattggta ttccagccga tgaaaaggca
      781 ataattatac ctatgcaaat gattaaaatt cgtatgagta atagccaact tcctatttta
      841 tttgtttttc cgttcgttag actttgtctt ttatcagaag ctaaaaaaga aacaattttt
      901 gaaatgaatg tcgaaataat aagtaccata aagaaaataa agacactgtt gtaggtgtaa
      961 ttaaaatcgc ctatgtttct gggtttagaa aattctaatt caaagggaga tattattttt
     1021 tgataccaat atgtatttcg tgcaacaagt ataaaccagc ctaatacaaa taaaaagaca
     1081 tattttttac ttattttgtg gtactcaatt gtttttaaat ttaatttctt ttcctcatcg
     1141 gaatccttta agtattcaga atataaacca atatcttttg ttaaatgaaa agtactcaat
     1201 aacataatag caataaaaat attatatagt cctatggtta ttatgacttt agcaaaatta
     1261 taattgccta aaagcataaa cacaattcca actacttcta aaattatcat aaggacaaca
     1321 atccaattca ttagttcgct atcccatttt ttacgatttt taagagtatg taatccaaat
     1381 aatatagaag ttgaagcaat aaacactaaa aaccatgatt ctgataccga gtgaagaaga
     1441 atcgtattgt caaaaatggc caataaattc agaatgaaaa taccaatcca aaaagtataa
     1501 taataaacag aagttgtttt tttgaataat atggttaaag aaattcctat tactgtccaa
     1561 aaaatagcca tcaaagcaaa tggtggttgt ggtaaaaaaa attgaaataa agtaaaaaca
     1621 attacaatgg aggaagaaaa aggatgtctt aaaatttgaa cagggtaatt aaatttgtca
     1681 tatagattag ctttttcgta ttttttcttt agaagtttta agtaaaatgc cgttgccaaa
     1741 aatgcaagaa atataataat gaagaaatca aaatgattga aagcataaaa gagcaataat
     1801 aatatttctt ttttaagaga ataaataaat gtagaaacaa aattttcttt ttcttggctt
     1861 tttgaaaaca agttgaattt tttagaaaat aatgcgtcta gttcggtttt ttttaaatta
     1921 ttaatttcaa tgatatctga ttgtaaattg aatttaaatt tcgaacccag tatttcaagt
     1981 ttatgaatac tgtctaaacc atttcttaaa agagagttga tttctttata atcaccattt
     2041 aattgcatga attgattaaa atagattgtt ctggcagcag tatctttagg cgaaatatag
     2101 agctctttag agataaataa agaatcaatt ttattttgac tgctacttaa aacatcatta
     2161 ttccctttta ttttaattaa ctgattttca attcgatttg aaatttcatt caacattaag
     2221 gaagtgactg ataaatttcg ataggttaga taattttcat cttgatcaat aa
//
LOCUS       DAOTCW010000239         2247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_41739_length_2247_cov_47.036953, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000239 DAOTCW010000000
VERSION     DAOTCW010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41739_length_2247_cov_47.036953"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="P5335_11840"
     CDS             complement(<1..1294)
                     /locus_tag="P5335_11840"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRZ75617.1"
                     /translation="MLAEYFLETDDIESSQRWLEKTKDLVSPKVIDSTAVFIQSLQSE
                     LFYYMGLYQFGTNEAEKAIEKARQIKDSLLIADAYFFLGINQLEINELEKAQQSLWKS
                     RNYFPNKTDRKHIRSLIQKEHIYNNIAQSKFKLKEMDSAVWYNAKAYTFAKKNNSKRG
                     IPNIEQTFGQIYLSKNQKDSALFYFQKSIHSAIKSNYYDIVLLNFSYLMESQSDDQVL
                     VNQWFEKGMGLMDQKIINITFQRYFYVTALAVFKKRNQTDKVAFVQEKIIAIDDETQL
                     KGNFYIQNITEQYAKNENKLLTLQVEELTKQRKYAFLQLIAAGLSLIILSLVIVIIRR
                     KNKIQQTLLNQKSEISKDLHDDIGSGLSSILIHADLLLRSGETSDKQKVLATKINQTG
                     KEISQRLNTFIWSLNTEHNTLQHFSEYVKFYGTTLFEET"
     gene            complement(1439..2065)
                     /locus_tag="P5335_11845"
     CDS             complement(1439..2065)
                     /locus_tag="P5335_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRZ75618.1"
                     /translation="MKKRIGIVEDDRDLREALGMMIQFTDQYELAGSFENAELALEEL
                     PKLTVNAILMDINLPGESGIICVNKLKSICPSVLVLMCTSYEDDEKIFQSLEAGASGY
                     ILKTEGPAKIIGALDELFEGGSPMSSSIARKVVASFSKMESQNKITESLTAREKEVLE
                     LLAKGQMNKEVAAQLEISNGTIRKHIQNIYEKLHVNTRVEAVNLYLKR"
BASE COUNT          692 a          413 c          316 g          826 t
ORIGIN      
        1 gtgtttcttc aaaaagtgtt gtgccataaa acttaacata ttctgaaaag tgttgcaacg
       61 tattatgttc tgtatttaat gaccaaatga acgtatttaa ccgttgtgaa atttctttcc
      121 cggtttgatt aatctttgtg gctaatactt tttgcttatc actggtttca ccgcttctta
      181 ataataaatc tgcgtgtatt aaaatactgc ttaaaccact tcctatatcg tcatgtaaat
      241 ctttactgat ttcacttttt tgattaagta atgtttgctg aattttattt tttcttcgaa
      301 taataacgat cactaaagaa agaataataa gtgataatcc ggcggctatt aattgcaaaa
      361 aagcatattt tcgttgcttt gttaattcct caacttgtaa ggtcagtaat ttattctcat
      421 ttttcgcgta ctgttccgtt atattttgaa tataaaaatt gcctttcagt tgagtttcat
      481 catcaatggc tataattttt tcctgcacaa aagccacttt atctgtttga ttcctctttt
      541 tgaaaacagc caaagcagtt acataaaaat agcgttggaa tgtgatgtta atgatttttt
      601 gatccattaa acccatgcct ttttcaaacc attggttaac taatacttga tcgtctgatt
      661 gactttccat caaatagctg aaattcaaca atacaatatc ataatagttg cttttaatag
      721 cagaatgaat acttttttga aagtaaaaca aagcactgtc tttctggttt tttgaaagat
      781 aaatttgtcc aaaggtttgt tctatattgg gaatacctcg tttactgttg tttttcttag
      841 caaatgtata tgcttttgca ttataccaaa ccgcagaatc catctccttc aatttgaatt
      901 ttgattgggc aatattatta tagatatgtt ccttttgaat aagacttcga atatgttttc
      961 tatcggtttt attaggaaaa taatttcttg atttccacaa agattgttgt gctttctcca
     1021 actcattaat ttcaagttga ttaattccta aaaagaaata ggcatccgca ataagtaagc
     1081 tgtctttgat ttgccttgct ttttctatag ctttctctgc ttcattggtt ccaaactgat
     1141 acaatcccat gtaataaaaa agctccgact gcagactttg gatgaataca gccgtgctgt
     1201 caataacttt tggagacact aaatcttttg ttttttcgag ccatctttga gacgactcaa
     1261 tatcatctgt ttctagaaaa tactcggcta acaaaaaaga ttgctttata cgacactttt
     1321 cagttttaca attttgaaga tttttcaata acggttgatc atcggaatgt tgagcccatc
     1381 caactatgca aaagaaaaaa aaatataact gtaaaataaa tttcattgag ggacggaatt
     1441 atcgttttaa atataaattc acagcctcaa ctcgagtatt tacatgtagt ttttcgtaaa
     1501 tattctgaat gtgtttccga atcgtaccgt tactgatttc taactgagcg gctacttctt
     1561 tattcatttg cccttttgcc aacaattcta acacttcctt ttcacgagca gttaatgatt
     1621 ctgtaatctt attttgggat tccattttag aaaaactcgc caccacttta cgggcaatac
     1681 tactactcat aggacttcct ccttcaaata actcatctaa tgctccaata attttagccg
     1741 gaccttccgt ttttaaaata taaccactcg caccggcttc caaactttga aatatttttt
     1801 catcatcttc ataagaagta cacatcaata ctaatacaga agggcaaata ctcttgagtt
     1861 tattcacaca gatgattccg ctttcacccg gcaaattgat atccattaaa atagcattta
     1921 cggtaagttt aggcaattct tctaaagcca gctccgcatt ttcaaaactt ccggccaact
     1981 catattgatc cgtaaattga atcatcatcc ctaatgcttc acgcaagtcc ctatcgtctt
     2041 ctacaatgcc aatgcgtttt ttcattttac ttaaatttac tatgcaatat taaaaatagt
     2101 aattcttttt tgaaatacgt aaataggcgt agtaaaataa aaaactactt attggttaac
     2161 gattgaattt ttaagaaatt gtaatgcata cactctaatc gcaataaaaa aacctcccat
     2221 ttctgagagg ttttgtaccc ggagtgg
//
LOCUS       DAOTCW010000240         2247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_41740_length_2247_cov_42.193431, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000240 DAOTCW010000000
VERSION     DAOTCW010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41740_length_2247_cov_42.193431"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="P5335_11850"
     CDS             <1..818
                     /locus_tag="P5335_11850"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRZ75619.1"
                     /translation="QNSSNCECESLVLTFFPHPRMILQDSQEIKLLNTIEERIQLLEA
                     SGLDNLVIHPFNQEFSRLTAEEFVSTVLVDQFNVKKIIIGYDHRFGRNRTADIYDLIE
                     FGKNYEFEVEQISAQEIDEVSVSSTKIRKALLEGNVALANEYLGYAYLLTGTIVKGKG
                     IGRTINFPTANLKIEESYKLIPKKGVYIIQSTINNRLVFGMMNIGYNPTVNGEGQNIE
                     IHFFDFKENLYTKKIQIQVLKRIRDEEKFDSLEQLTQQLEKDKSTALNYLNTL"
     gene            818..2149
                     /locus_tag="P5335_11855"
     CDS             818..2149
                     /locus_tag="P5335_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HRZ75620.1"
                     /translation="MLEKAYRPIDNAPLILFRIALGALIMVESFVAIFTGWVKKNLID
                     PTVHLPHIGFDFLIPLPGLGMYAYYCIMGIFGLGILLGYKYRLSLAGFTLMWTGVYLM
                     QKASYNNHYYLLLLVCLIMLFLPANRYASLDAKSNPKIKSLSMPQWVAWVMIAQMSIV
                     YFFATISKFYPDWLNGTFTGILFSNISDFPVIGEVFTEKWFHLFIAYTGIFFDGLIIP
                     ALLWKKTRNLAFLASLIFHLFNSITLLIGIFPYFALSFVVFFYPPETIRAIFFKRKPT
                     LIQENLTNYSHTKALTFFFIPFFIIQLILPVRHWFIKGDVLWTEEGHRLSWRMMLRDK
                     SGLTTFKIIDKKTKEELKYNLAEHLTKKQLVFVSSKPDGMWQMAQHIKKEFGKQGKEV
                     KIYIHSQLSVNGKPYKLFIDPTVDFGEAKWNYFGHNEWIILYDKNGNRISN"
BASE COUNT          769 a          360 c          382 g          736 t
ORIGIN      
        1 ttcaaaactc ctcaaattgt gaatgtgaaa gtttagtact tacttttttc cctcatccca
       61 gaatgattct tcaggatagc caggaaataa aattattgaa caccatagag gaacgcatac
      121 aattattaga agcttcaggg ttggataatt tagtaattca tccctttaat caggaatttt
      181 ccagactaac tgcggaagaa tttgtttcta ctgtgttagt tgaccaattt aatgtcaaaa
      241 aaataatcat aggatacgac catcggtttg gaagaaacag aactgcggac atctacgact
      301 tgattgaatt tggaaaaaat tatgaatttg aagttgaaca aatttctgct caagaaattg
      361 atgaagtttc tgtaagttca accaaaatca gaaaagcatt attggaagga aatgttgctc
      421 tggcgaatga atatttaggt tacgcctatt tattaaccgg aacgattgtt aaaggaaaag
      481 gaattgggag aacgataaac tttcctacgg caaatttgaa aattgaagaa tcatataaac
      541 tgattcctaa aaagggtgtt tatataattc aaagcaccat aaataatagg ttggttttcg
      601 gcatgatgaa cattggatat aatccaacag taaatggaga agggcaaaac atcgaaattc
      661 atttctttga ttttaaagaa aatttatata ccaaaaaaat acaaattcaa gtactaaaac
      721 gaattcgaga tgaggaaaaa tttgacagtc tggaacagtt gacccaacaa ttggagaaag
      781 acaaatctac tgccttgaat tatctcaata cactatgatt cttgaaaaag catatcgccc
      841 gatagacaat gctcctttaa ttttatttag aattgcatta ggtgctttaa ttatggtgga
      901 aagttttgtg gctattttta caggttgggt aaaaaagaac ttaatagacc caacggtcca
      961 tcttccccat atcggatttg attttttaat tcccctcccc ggattaggga tgtatgctta
     1021 ctattgcata atgggcatct ttggattagg catactacta ggttataaat atcgcctaag
     1081 tttagcgggt tttactttaa tgtggaccgg cgtatattta atgcaaaaag catcctataa
     1141 caatcattat tacctattgc ttttagtttg tttaattatg ctttttttac cggctaatcg
     1201 ctatgcatct ttagacgcaa aaagcaatcc taaaataaaa agtttatcaa tgccacaatg
     1261 ggtcgcttgg gtaatgatag cacaaatgtc aatcgtttat tttttcgcaa ctatttcgaa
     1321 attttatccc gattggttaa acggcacttt cactggaatt ttattttcaa acatttctga
     1381 tttccctgta attggtgaag tttttactga aaaatggttt catttgttta ttgcctatac
     1441 cggtatcttt tttgatggct taatcattcc ggctttactc tggaaaaaaa caagaaattt
     1501 agcattcctt gctagtttga tttttcattt atttaattcc attacgcttc taatagggat
     1561 tttcccttat tttgcgttga gctttgtggt ttttttctat cctccggaaa ctattcgtgc
     1621 cattttcttt aaaagaaaac caacgcttat tcaagaaaat ttgacaaatt attcacacac
     1681 taaagcacta acctttttct ttattccttt tttcattatt caactgattt taccggtaag
     1741 gcattggttc attaaaggag atgtcttgtg gacggaagaa ggtcatcgcc taagttggag
     1801 aatgatgctt agagacaaaa gcggtctcac tacatttaaa ataatagata aaaaaacaaa
     1861 ggaagaactg aaatacaacc ttgctgagca tctgacaaaa aaacaacttg tttttgtgag
     1921 cagcaaaccg gatgggatgt ggcaaatggc acaacacatc aaaaaagaat ttggaaaaca
     1981 aggcaaagaa gtaaaaatct atattcattc ccaactttct gtaaatggca aaccatacaa
     2041 attatttata gatccaacag ttgattttgg agaagcaaaa tggaattatt tcggtcataa
     2101 cgaatggatt attctttatg acaaaaatgg aaatagaata tcaaattgaa agattcactc
     2161 acaatctatt cctatcgttt ttttataact gacataaact aaataaaaaa acctccgatt
     2221 gctcggaggt ttctattgta atgtaag
//
LOCUS       DAOTCW010000241         2222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_42459_length_2222_cov_39.425934, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000241 DAOTCW010000000
VERSION     DAOTCW010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42459_length_2222_cov_39.425934"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="P5335_11860"
     CDS             complement(<1..409)
                     /locus_tag="P5335_11860"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRZ75621.1"
                     /translation="MKKILSITVLTLLLAACSGETKNGNLDEVIQSKDLAKIKSSRES
                     VHKEYEKLVADLAKLDKAIAELDTNKKNPLVQTITLKDTLFTHYIEIQGNVDTKENIM
                     INPEYSGILTQVNVKAGQRVSKGQVLARIDDGGM"
     gene            complement(422..1762)
                     /locus_tag="P5335_11865"
     CDS             complement(422..1762)
                     /locus_tag="P5335_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRZ75622.1"
                     /translation="MNKFALFLLLTCVGIQAQETSKKSYSFSLQQAIEHAIENNYQSI
                     NSGRDIESAQQKRKEATALGLPQVNATVDFQNNFVIQKSVVPAEFFGGNPGEFVEVAF
                     GTKQNMNARASLNQLIFDGSYIVALQASKTYLDFYEKSKAKTDLEIKEMIINSYGNVL
                     LSEESINILKSNITSVEKTLFETEQTFKNGLTEEENVEQLNIRLTTLKSSLNYSNRLR
                     DIAQNMLKINLGIELEDELTLTDDLKSLTALNLDLALTNPEFNVSQNIDYTLMTNFVD
                     QRNLELKLEKSRYLPSLGASLNYGYNAFGEDFSTFTDADNWLDYSNLGLNLNIPIFSG
                     LGRNARTQQAKIALEQAKTELTETEQRLKLEYLTAKSKFDFDVEQYFSSKQNLTLAER
                     IEKKQNIKFTEGLSSSFEFTEAQRQLYTAQQDYLQAMVNVINSRAALEKITTKN"
     gene            complement(1777..>2222)
                     /locus_tag="P5335_11870"
     CDS             complement(1777..>2222)
                     /locus_tag="P5335_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRZ75623.1"
                     /translation="TAVHFQIHEAIQKIASQGLNAIEENFVIRKMFKEMFNTSETSPI
                     FQLKKHYPEIYEKVISREINECNSFFTHNVEKGIQQGLYRKDLNIPSCVKFYYTLIFS
                     IKESTISEKESLDLEYQALEYHTRAISTPDGIQELEKQLEKKKSS"
BASE COUNT          614 a          374 c          386 g          848 t
ORIGIN      
        1 tcattccgcc gtcgtcaatg cgagctaaaa cttgaccttt tgaaacacgt tgaccggctt
       61 ttacattcac ttgcgtcaaa atacctgaat attccggatt aatcatgatg ttttccttgg
      121 tgtcaacatt tccttgtatt tcaatgtaat gcgtaaaaag ggtgtcttta agtgtgatgg
      181 tttgaaccaa cggatttttt ttgttggtat ccagttcggc tatggcttta tccagtttag
      241 ccaaatccgc caccaatttt tcatattctt tatgaacgct ttcacgtgaa gatttgattt
      301 ttgctagatc ttttgattgg atgacttcgt caagatttcc gtttttagtt tcgccggaac
      361 atgctgctaa taaaagcgta aggaccgtta tggaaagtat ttttttcatt gggaggtgtt
      421 attagttttt agttgtgatt ttttctagtg ctgctctact gtttatgaca tttaccattg
      481 cttgtaaata atcctgttga gcggtatata attgtctttg agcttctgta aattcaaaac
      541 tggacgaaag accttcggtg aattttatgt tctgtttctt ttcgatacgt tcagccagcg
      601 tcaagttttg tttggaacta aaatattgtt ccacatcaaa atcaaatttg cttttagcgg
      661 ttaagtactc cagttttaaa cgttgttctg tttctgtgag ttccgttttt gcttgttcca
      721 aagcaatttt cgcttgttgc gtacgagcat ttctgcctaa accactgaaa attgggatgt
      781 tcaaattcaa tcctaaattg gaataatcca accaattatc tgcatcagta aacgtactga
      841 aatcttctcc aaatgcattg tagccataat tcagggaagc tccaagagac ggcaaatatc
      901 tacttttctc gagttttaat tccaggtttc gttggtcaac aaaattggtc attaatgtat
      961 aatcaatatt ttgggacaca ttaaattccg ggttggtaag agccaaatca aggtttaaag
     1021 cagttagtga ttttaaatca tccgttaaag tcagttcgtc ttccagttca atacctaaat
     1081 tgattttcag catattttga gcaatgtctc tcaaacgatt actgtaattc aaactgcttt
     1141 ttaaggtagt gagtcggatg tttaattgct ctacattttc ttcttctgtt aaaccatttt
     1201 tgaacgtttg ttcggtttca aacagtgttt tttcaacaga tgttatgttg cttttcagaa
     1261 tgttaattga ttcctcagaa agcaaaacat ttccatagga attgataatc atttctttga
     1321 tttccaaatc ggttttcgct tttgattttt cataaaaatc caaatacgtt tttgaggctt
     1381 gcaaagcaac gatataggat ccgtcaaaaa ttaactggtt caacgatgct cttgcattca
     1441 tgttttgctt ggttccaaaa gcaacttcaa caaattcacc cggatttccg ccaaaaaatt
     1501 cagcaggaac aactgatttt tgaatgacaa aattgttttg aaaatcgact gtggcattta
     1561 cttgaggtaa tcccagtgcg gtagcttcct ttcttttttg ttgtgcagat tcaatatctc
     1621 tacccgaatt aatcgattgg tagttgtttt ctattgcatg ttcaattgct tgttgcagtg
     1681 aaaatgaata gcttttttta gaggtttcct gagcttgtat tcccacacaa gtcaacagga
     1741 gaaagagtgc aaatttattc atatagtgat tgatgattaa gatgatttct ttttttctaa
     1801 ttgtttttct aattcttgaa ttccatcggg ggttgatatt gcccttgtgt gatattctaa
     1861 tgcttgatat tctagatcta gtgattcttt ttctgaaatt gttgactcct tgatactaaa
     1921 gattaatgta taataaaatt ttacacaaga aggaatgttg agatcctttc gatacaaccc
     1981 ttgttgaatt cctttttcaa cattatgagt aaaaaaacta ttacactcat taatttcacg
     2041 ggatattact ttttcataaa tttcaggata atgtttttta agttgaaaaa ttggtgatgt
     2101 ttctgaagtg ttaaacattt ctttaaacat tttccgaatc acaaaattct cttcaattgc
     2161 atttaatcct tgggaagcaa ttttttgaat cgcttcgtgt atttgaaaat gaacagctgt
     2221 tg
//
LOCUS       DAOTCW010000242         2208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_42861_length_2208_cov_33.991640, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000242 DAOTCW010000000
VERSION     DAOTCW010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42861_length_2208_cov_33.991640"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..820)
                     /locus_tag="P5335_11875"
     CDS             complement(239..820)
                     /locus_tag="P5335_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HRZ75624.1"
                     /translation="MLGLKLKTDTRWANIAESNLEEILSDHCWCEQKAASNALYIITN
                     NSENVELVTEMAKIAIEEMEHFQMVHNIIQERGFEFGREVKDDYVNQLVKFTKKGGTR
                     NDALIERLLFAAMIEARSCERFKVLSKNIKDKKLADFYFKLMISEANHYSTFLNFARK
                     YSEKIDVEKRWQEWLEFEGELISNYGKKETVHG"
     gene            896..2116
                     /locus_tag="P5335_11880"
     CDS             896..2116
                     /locus_tag="P5335_11880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRZ75625.1"
                     /translation="MNAFENKRILIIGFVWPEPKSSAAGSRMMQLISFFKEKGAKITF
                     ASTAAESEFSEDLEGVDQKLIALNCTSFDDFVSELQPDLVLFDRFMTEEQFGWRVAEH
                     CPNALKMLDTEDLHCLREARRLVVLKHKPFELNNDLTKREIASIFRCDVTLMVSEFEM
                     ELLQTYFRVPSFLLFYLPIWVENETLDLPSFEERDDFVFIGNFLHAPNHDTVIYLKEE
                     IWPLLSKKFPEAKLQIYGAYPTPKIKSLHQPKLNFFVHGRAESSFEVISKARVLLAPI
                     RFGAGIKGKLLEAMQFGTPSVTSIIGAEAMNGDFLWNGFITDSQEDFAEKAIEFYQNK
                     SIWVKAQQNGFEIIKNRFSKDLFENTFLEFVLDKFENLGNYRNDNFTGLLLMHHFLQS
                     TKYMAKWIEEKNKQ"
BASE COUNT          675 a          314 c          430 g          789 t
ORIGIN      
        1 ctgcaatttg tgggagagta tgtcgccgcc ttctttttaa aatcctcatc atttagttga
       61 tgaggatttt tttgttatat attttttaga aaaaatttat tctcgaagaa atgaatcaat
      121 attaaacctt tggtttaatg aaaaatacag taattaattt aagtaatcta aactacaata
      181 attaaatgtt ttttaaggct tgaagcatat gaaactagtt gtttagttta attcataatt
      241 aaccatgaac agtttctttt ttaccatagt ttgaaataag ttctccttcg aattctaacc
      301 attcttgcca acgtttttct acatctattt tttcagaata cttacgggca aagtttaaaa
      361 aagtgctata atggttggct tcactgatca tgagtttaaa ataaaaatca gctaattttt
      421 tgtctttgat gtttttggac agcactttga aacgttcgca actccgagct tcaatcatag
      481 ctgcaaagag caatctctca attaaagcat catttcttgt tccacctttt ttggtaaatt
      541 taacgagctg gttgacataa tcgtctttta cttctcgacc aaactcaaag ccacgttctt
      601 gaatgatgtt gtgcaccatt tgaaaatgtt ccatttcttc gatggcaatt ttggccattt
      661 cggttactaa ttcaacattt tcagaattgt tggtgatgat gtaaagtgcg ttagaagctg
      721 ctttttgttc gcaccagcaa tgatcggata aaatttcttc gaggtttgat tcggcaatat
      781 tggcccaacg tgtgtcggtt ttgagtttta atcctaacat gattcacttg atttgataca
      841 aaaataatgc tttttatttg aatagaattg gctcattaat tactatttta gtgcgatgaa
      901 tgcatttgaa aataaaagga ttttaattat tggttttgtg tggccggaac cgaaatcatc
      961 ggctgccgga agtaggatga tgcaattgat ttcttttttt aaggaaaagg gagcaaaaat
     1021 tacttttgcc agtacagctg ccgaatctga attttcagaa gatttggaag gtgttgacca
     1081 aaaattaatt gcattgaatt gtacttcgtt tgatgatttt gtgtcagaac tacaaccaga
     1141 tttggttttg tttgatcgat ttatgacgga ggaacaattt ggttggcgag tagcagaaca
     1201 ttgtccgaat gctttgaaaa tgttagatac ggaagatttg cattgtttac gggaagcgag
     1261 acgtttagtg gttttaaagc ataaaccgtt tgagttgaac aatgatttaa caaagcgtga
     1321 aattgcgagt atttttcgtt gtgatgtgac gttaatggtt tcggaatttg aaatggaatt
     1381 gttgcaaacg tattttagag tgccttcgtt tttattgttt tatttgccga tttgggtgga
     1441 gaatgaaaca ctagatttac caagttttga agaaagagac gattttgtat ttattggaaa
     1501 ttttttacat gctcctaatc acgatactgt tatctattta aaagaagaga tttggccatt
     1561 attatctaaa aaatttcccg aagcaaagtt gcagatttac ggtgcttatc ctacaccaaa
     1621 aataaaatca ttgcatcagc cgaagctgaa tttttttgtt catggtcgag ccgaaagttc
     1681 gttcgaagtg atttctaaag caagagttct tttggcaccc attcgttttg gtgccggaat
     1741 taaaggaaaa ttgttggaag ccatgcagtt tggcactccg agtgtaactt caataattgg
     1801 tgcagaagca atgaacggcg attttttgtg gaatggtttt attaccgatt cacaggaaga
     1861 ttttgctgaa aaagcgatag aattttacca aaacaaatca atttgggtga aagctcagca
     1921 aaatggtttt gagattataa agaatcgttt ttcgaaagat ttgtttgaaa atacttttct
     1981 agaatttgta ttggataagt ttgaaaattt gggaaactac cgaaatgata attttaccgg
     2041 tttattattg atgcatcatt ttttgcagag cacgaagtat atggcgaagt ggattgagga
     2101 gaagaataaa caataaaaaa gcgagttcaa tttgagctca cttttttatt gtttattcgg
     2161 ctaaatctat gccaacatag tcaccggatt ttctaaataa gttcgcaa
//
LOCUS       DAOTCW010000243         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_42957_length_2205_cov_32.387442, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000243 DAOTCW010000000
VERSION     DAOTCW010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42957_length_2205_cov_32.387442"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="P5335_11885"
     CDS             complement(<1..773)
                     /locus_tag="P5335_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75626.1"
                     /translation="MKIAYLLDCYDVLLRQVKSNATVSNKVILNELVKKEDDSYRIYK
                     VTIIKREGNETIKISKSKHSFHPEIQSLELKTLFQFNEFENYIPRLKNLIEFTGETIL
                     FAGDPQNENIFYVYEKCSISELNPKERFFLYCNQVLKEENQNIKLAIKEKVFKFKSKI
                     KIEHYIHKNQLAMETQLNNLIKQINPESNSELYQFSNQYDKSDCLKSIYFNLEKLLLF
                     TEKEYNEYLNVNIMVPQRTVLMNEYAIKPKLDFVRDSLLA"
     gene            complement(944..2197)
                     /locus_tag="P5335_11890"
     CDS             complement(944..2197)
                     /locus_tag="P5335_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRZ75627.1"
                     /translation="MKHKLSILFFVKSTKATKDGLLPIYQRITINGNRIELSTSRLVE
                     KTKWNSSGGKIRGSSVEARLINSHLDMLRNKVFEIEKWMVNNNEEITAQTFKNKLLGI
                     DEKERKLIVIFEEHNKRIAQLIGKEYSNNTYKKYETTISHTKEFLKYQFNVTDISIKK
                     VDIGFINDFDFYLRKVKNCNNNSTIKYIRNFGKIVKQCYANGWIDKDPFLNYKGKVKE
                     VDRVYLSEEEIQEIINKDFGTDRLSLVRDIFLFSCFTGLAYIDVKNLTKSHISIGIDG
                     EKWIFTHRQKTESASKIPILPVTQMIINKYENHPQACNQDRLLPILSNQKMNAYLKEI
                     AGVCKINKELTFHIARHTFATTVTLTNGVPIESVSKMLGHKNLRTTQHYAKVLDKKVS
                     EDMKILKDKFSNIMIAKELKNQDVS"
BASE COUNT          627 a          335 c          347 g          896 t
ORIGIN      
        1 gccagcaatg aatctctcac aaaatccaat ttaggtttta tcgcatactc attcattagt
       61 accgttcgtt gaggtaccat gatgtttaca tttaaatatt cattgtattc cttttcagta
      121 aacagcagta atttttccaa attaaaataa atacttttca aacaatctga tttgtcatat
      181 tgatttgaaa attgatacaa ttcactatta ctttccggat taatttgttt gatcaagtta
      241 ttcaattgcg tttccattgc caattggttc ttgtgaatgt aatgttcaat tttaattttt
      301 gatttgaatt tgaacacttt ttctttaatg gccagtttta tgttttggtt ttcttccttt
      361 agcacttgat tgcaatacaa aaaaaatcgt tctttcgggt tgagttcact tattgaacac
      421 ttttcataca cataaaatat gttttcattt tgtgggtctc cagcaaacaa aattgtttca
      481 ccggtaaatt cgataagatt ttttaatcta ggaatatagt tttcaaattc attgaattga
      541 aaaagtgtct ttagctccaa actttgaatt tccggatgaa aagaatgttt tgatttactt
      601 attttaattg tttcattacc ttcacgtttg atgatggtaa ccttgtaaat acgatatgaa
      661 tcatcttctt tcttaaccag ttcgttaaga atgactttat ttgaaacagt agcatttgat
      721 tttacttgac gcaataaaac gtcatagcaa tcgagtaaat aagcaatttt catagtaggc
      781 aataaaaatt cctgtgttaa aataacacgt tagttaattg aagaatcaaa tttgtttttg
      841 gaatttttag tcatttaaca acactaaggg cagtagtgtt gttaaatttg tcgcaattta
      901 aaaatttatt tacaattgtt taacttttcg tacaaaaaat cgattaactg acatcttggt
      961 tttttaactc tttagcaatc attatgtttg aaaatttatc ttttagtatt ttcatatcct
     1021 cactcacttt tttatccaat actttggcat aatgttgggt tgttctgagg tttttatggc
     1081 caagcatttt acttaccgat tcaataggca cgccgtttgt gagcgtcaca gtagtggcaa
     1141 aagtgtgacg ggcgatgtgg aatgttaatt ctttgttgat tttgcaaaca ccggctatct
     1201 cttttaaata agcattcatt ttttgattgg aaagaatggg taaaagtcta tcctggttgc
     1261 aggcttgtgg atgattttca tatttgttga ttatcatctg cgttaccggt aggattggaa
     1321 ttttggaggc actttccgtt ttttgacggt gggtgaatat ccatttctcg ccatcaattc
     1381 caatgcttat atgtgatttg gttaggtttt tcacatcgat ataggctaag cctgtaaagc
     1441 agctgaaaag gaagatatca cgaactagtg aaagtcggtc ggttccgaag tctttgttaa
     1501 tgatttcttg gatttcttct tcggataaat atactctatc tacttctttg acttttcctt
     1561 tataatttaa gaatggatct ttatctatcc atccatttgc gtaacattgt ttaacaattt
     1621 tgccaaagtt tctgatgtac tttatagttg aattgttatt acaattttta acttttcgta
     1681 agtaaaaatc gaaatcattg ataaaaccaa tatcaacctt cttaattgat atatcagtga
     1741 cattgaactg gtatttaaga aattctttgg tgtgactgat ggttgtttcg tattttttgt
     1801 atgtgttatt cgaatactct ttgccaatta gttgagctat tcttttgttg tgttcttcaa
     1861 atatcacaat tagttttctt tccttttcat caatgcctaa gagtttgttt ttgaaagtct
     1921 gtgcggttat ttcttcattg ttattaacca tccatttttc aatttcaaaa actttatttc
     1981 taagcatgtc gagatgacta ttaattagac gtgcttcaac actggaaccc ctaattttgc
     2041 ctcctgagga attccatttc gttttttcta caagtcgtga agtacttagt tcaattctat
     2101 ttccgttaat tgttatcctt tgataaattg gtagcaatcc atccttagtg gcttttgtac
     2161 tttttacaaa aaaaagtata gataatttat gtttcatttt aggta
//
LOCUS       DAOTCW010000244         2181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_43642_length_2181_cov_43.093133, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000244 DAOTCW010000000
VERSION     DAOTCW010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43642_length_2181_cov_43.093133"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..802)
                     /locus_tag="P5335_11895"
     CDS             complement(35..802)
                     /locus_tag="P5335_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75628.1"
                     /translation="MKNIMAFFAMVLILTSCEKIEDDTDNDCISNCATLSGKFVTLNN
                     EPVPNIKVSFKYTISGGELGGGSTRKIVNEESDQNGKFYKNFFIKDSELGNSAQGYFL
                     FEVDDSSLDVNKYIRTNNLIGNTTTPIGFAIFPITNRDTIIDNTFYIPKKAYIKVNLN
                     NFVPLQDDDFFEVQTLYPFGTKIGTNPFLNSEYQTGFSGYGNWRATANNTLLNIFVAE
                     GENNMVRIFRRKNGENTIEDIPIFIPVNNTIELTFDY"
     gene            complement(963..1883)
                     /locus_tag="P5335_11900"
     CDS             complement(963..1883)
                     /locus_tag="P5335_11900"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRZ75629.1"
                     /translation="MKSTQEDKLSMEIVVVDLINNTDPTIVAKMPNFDSLSTDFSKNV
                     DKLREFKNGQTLNRTGNRMSKEQLREQMTQLGFITSAKVCAYAIAIDDEVLEMEVTYT
                     YSNLQYLRDTDIADTCQSIYEIAQANLANLTDYGVTAASLLELKDSIDLYNVMLPKTR
                     AGIVTQTIFTDKIVEVFEENEKLLFRMDKLVTMLKFEEATFFDEYFKSRKIVSTGHRK
                     LSLKGKITNELGQPIDNVTITIETPTPETTKSTELGNYQFKTAIAGVFPVTFKRDGYI
                     TERVFLVFTPNVRVDFNVVLKLAEEQQRSA"
BASE COUNT          595 a          318 c          422 g          846 t
ORIGIN      
        1 acgaagttgg tagacttggt ggtgatggtt tttattaata atcaaaagtc aattctatgg
       61 tgttattgac gggaataaat attggaatat cttcaatcgt gttttcccca ttttttcttc
      121 taaaaatcct caccatattg ttttcgcctt cggctacaaa tatattcaaa agagtattat
      181 tagctgttgc tctccaattt ccataaccac taaaccccgt ttgatactct gaatttaaaa
      241 aaggatttgt tccaatttta gtaccaaaag gatataatgt ttgcacttca aaaaagtcat
      301 cgtcttgtaa gggtacaaaa ttgtttaaat ttacttttat atatgctttt ttaggaatat
      361 aaaaagtatt gtcaattatt gtatctctgt ttgtgatggg aaaaattgca aaaccaatag
      421 gagttgttgt attaccaatt aagttatttg ttctaatata tttattgaca tcaagactag
      481 aatcatctac ctcaaataga aaatagcctt gagcagaatt tcccaattca ctgtctttta
      541 taaagaagtt tttgtaaaac ttgccgtttt gatctgattc ttcgtttaca atctttcgtg
      601 tagaaccgcc acctaactca ccaccactaa ttgtatattt aaatgaaact ttaatatttg
      661 gaacaggttc gttatttaag gtaacgaatt ttccgcttaa agtggcacag tttgaaatac
      721 aatcattatc tgtatcatct tctatttttt cgcaacttgt tagtattaat accattgcaa
      781 aaaacgccat tatatttttc atagaactat attaaatttt agatttagtt tatcaattct
      841 aaacgaatga ttcaaattta gttaaataga ttagattatt ggttggtttt ttactttatc
      901 gggaaaaaaa atcctgtttc gaggggaaac aggattttgt gttgtgcaaa gatttgcttt
      961 ttttatgcgg aacgttgttg ctcttcggct agttttagga caacgttgaa atctactctt
     1021 acgtttggcg tgaatactaa aaagactctt tcagtgatgt atccatcgcg tttgaaggtt
     1081 accgggaaca cgcctgctat ggctgttttg aactggtagt tgcctagttc agtggatttg
     1141 gttgtttccg gtgttggggt ttcgatggtg atggttacat tgtcgattgg ttgccctaac
     1201 tcgtttgtga tttttccttt tagggagagt ttgcggtgac cagtgctgac gatttttctg
     1261 gatttgaagt attcatcaaa gaaggttgct tcttcgaatt ttagcatggt tacaagtttg
     1321 tccattcgga agaggagttt ttcgttttct tcaaatactt ctacgatttt atcggtgaag
     1381 attgtttggg ttacgattcc tgctcgagtt tttggaagca tgacattgta gaggtcgatg
     1441 ctgtctttta attcgagtaa tgaagcggcc gttacaccat aatcggttag gtttgcgaga
     1501 tttgcctgag cgatttcgta gatgctttgg caggtgtcgg caatgtcagt gtcgcgaaga
     1561 tattgaagat tagaataggt gtaggttact tccatttcca gtacttcatc atcgattgcg
     1621 attgcgtagg cacatacttt ggctgatgta atgaagccaa gttgtgtcat ttgttcacgg
     1681 agctgttctt ttgacattct gttccctgta cggttgaggg tttgtccgtt tttgaattct
     1741 cttaatttgt cgacgttttt gctgaagtca gtgcttagcg agtcgaagtt tggcatttta
     1801 gccacgattg taggatcggt gttgttgatt aaatcaacga ctacaatctc catagacaat
     1861 ttgtcttctt gggtagattt catgagcgtt tggggtttta aattattatt ctattatttt
     1921 ttgcactggt ctaattgtta gtgcattaca aatctaaaca catttttttc ttacaaccaa
     1981 aaaaaaatat gaaaaagtgt taaaaaagta tcttttttgc ttattattat gtaaaaatct
     2041 tacagtaatt gtcgttaaaa tgttataatt taacgattaa atgtattatt ttttagttta
     2101 ttattgcttt gaaagggttt tgaaagggga aatttgtagg atgaattatt gtttgttctt
     2161 tagtttttga ttcttttagt t
//
LOCUS       DAOTCW010000245         2178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_43735_length_2178_cov_49.884597, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000245 DAOTCW010000000
VERSION     DAOTCW010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43735_length_2178_cov_49.884597"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="P5335_11905"
     CDS             <1..915
                     /locus_tag="P5335_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRZ75630.1"
                     /translation="GVIDSPDIPLNVSRSYLQSDGNVKKISNYITRKVADKLKSLFNE
                     NRPDFEQKWNDIKIVLEYGMLSEDKFYEKAGDFVLYPTVDEKYFTLTELKEKIAVNQT
                     DKNGKLVVLYASNKEAQHGYIDIAKEKGYEVLLLDSPIVSHLIQKLEADNENLTFVRV
                     DSDHIDKLIQKEDTQISKLSDEESTKLKTVLEELVPKATYTVQLEPMDSTAAPFMITQ
                     PEFMRRMKEMSQTGGGGMFGMGNFPEMYNLVVNTNSDLATTILSTEDKSAQEHLVKQA
                     MDLAKISQGLLKGEELTAFVKRSFEMIK"
     gene            1050..1229
                     /locus_tag="P5335_11910"
     CDS             1050..1229
                     /locus_tag="P5335_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75631.1"
                     /translation="MFTKFKKSELLILFLAIALIFVSEFYYVVLDNPERALFIGLWPP
                     TMIGFLIYFNLKLKK"
     gene            1618..2064
                     /locus_tag="P5335_11915"
     CDS             1618..2064
                     /locus_tag="P5335_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75632.1"
                     /translation="MSILKKLDLFLANMGNLKAMQRNHTDTFTESKIENIKGEKIASS
                     ENGGIWIEFQELAEYHFMNVIIVGKKKIKTFNGCELVFVGKENEMKLKSDMQEIVSDF
                     SNISNRWITFLSFDITDLNIDFILNKEAEKVTLYFKKDSESFDILK"
BASE COUNT          822 a          306 c          357 g          693 t
ORIGIN      
        1 ggtgtgattg attcaccgga tattccattg aatgtttcgc gttcctattt acaatcggat
       61 ggaaatgtga aaaaaatttc caattacatc acgcgaaaag tggcagataa attgaaatca
      121 ttattcaatg aaaaccgtcc cgatttcgaa caaaaatgga acgatatcaa aattgttttg
      181 gaatacggaa tgttatcgga agataaattc tatgaaaaag caggcgattt tgttctttat
      241 ccaactgttg atgaaaagta tttcacctta accgaattaa aagaaaaaat cgctgtcaat
      301 caaaccgata aaaacggaaa attggtggtt ttatatgctt ccaataaaga agctcaacat
      361 ggttacattg acattgccaa agaaaaagga tacgaagtat tgttattaga tagtccgatt
      421 gtgtcgcatt tgattcagaa attagaagct gataatgaaa acctgacatt tgttcgtgtg
      481 gattcagatc acattgacaa attgattcaa aaagaagata cgcagatttc aaaattatcg
      541 gatgaagaat caaccaaatt aaaaaccgtt ttagaagaat tggttccaaa agcaacgtat
      601 actgtgcaat tagaaccaat ggacagcact gccgcaccct tcatgattac gcaaccggaa
      661 tttatgcgaa gaatgaaaga aatgagtcaa accggaggcg gtggaatgtt cggcatgggc
      721 aacttcccgg aaatgtacaa tttagtggta aacacaaatt ctgatttagc tactaccatt
      781 ctttcaaccg aagacaaatc tgctcaagaa catttagtga aacaagcgat ggacttggca
      841 aaaatctcac aaggattgtt gaaaggtgaa gagttgactg cttttgtgaa gagaagtttt
      901 gagatgatta aataagagga aagaatatag agaatagaag aaaggcctgt gagtgaaaac
      961 ttgcaggttt ttttattaac ttttacttta aaaaacttac aattatattt gtaactttac
     1021 atctcaaatc tatattaaaa caacaaagca tgtttactaa atttaagaaa tcagaattgc
     1081 ttattctttt tttagcaatt gcgttaatct ttgtttctga attttattat gtagtattgg
     1141 ataatcccga aagggcttta tttattggat tatggccacc aacaatgatt ggatttttaa
     1201 tttattttaa tttaaaactt aaaaaataaa tggaaaattt ggaccttata atattgacca
     1261 taatagtagt tatttttttc ttggcttttt ttgtctccac tcttaaggca ttcgaatatg
     1321 caacaaaatt agacaatccg gaaagaaaaa aacatggaat attatcccga attttaaatt
     1381 atttacaaaa tattgtaagc gagtaactat ctaaataata aaaattgtca ttgtaaaact
     1441 tatgaataaa aaagttttca ttagtaattt aaaaaaaata tctgcatcat aggtaaaata
     1501 cataccaact ttcaacatgc tttaattgac gggatgagtg atataaaatt aaataaaacc
     1561 tgagattgag taactatagg ttttttgtaa atttgaattc caaaacaact taatataatg
     1621 agtattttaa aaaaacttga tcttttcctg gcaaatatgg gaaatctgaa agccatgcaa
     1681 cgcaatcata ctgacacatt tactgaaagc aaaatagaga acatcaaagg agaaaaaata
     1741 gcctcttctg aaaacggagg aatttggatt gaatttcaag aactcgctga ataccatttt
     1801 atgaatgtaa ttatagttgg caaaaagaaa atcaaaactt ttaacggttg tgagcttgtt
     1861 tttgttggaa aagaaaatga aatgaaatta aaatcggata tgcaggaaat tgtttctgac
     1921 ttttcaaata tttccaatag atggattaca tttcttagtt ttgatataac tgatttaaat
     1981 atagatttca ttttaaataa agaagctgag aaggtaacac tttactttaa aaaagattct
     2041 gaaagttttg atattttaaa ataagcaaaa acaatcttgt aaaatttttt gaatttttaa
     2101 cgcacaacac ttaattttca tgctattaac catcgaaacc cctgccctac ttttctccgc
     2161 cacttccttg attttatt
//
LOCUS       DAOTCW010000246         2177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_43763_length_2177_cov_44.745052, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000246 DAOTCW010000000
VERSION     DAOTCW010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43763_length_2177_cov_44.745052"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="P5335_11920"
     CDS             complement(<1..543)
                     /locus_tag="P5335_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HRZ75633.1"
                     /translation="MRMENTLSTPNTLPSDLAVVKELVYDKCGFHFSNFKQNDESADY
                     GACSFELNGKKIQHRVSKITPTKKGQFVTLWKRNKDGVTAPFDLSDDIDFILITSKSG
                     DHFGQFIFPKSVLATKGILTKNGKEGKRGIRVYPPWDIATSKQAEKTQNWQTTYFLTI
                     KKDNTSELNLPKKLFTKTNEI"
     gene            complement(609..1130)
                     /locus_tag="P5335_11925"
     CDS             complement(609..1130)
                     /locus_tag="P5335_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75634.1"
                     /translation="MKNLNLIAGLLLILISFSCSSEEDDGNQAQNNGFNINGNFYPTQ
                     YAYISDENTIDNTISDISIILANVDPFQTNQSTGINYVFFDFPAVNIEPGTITNFPDY
                     QILENANLNNFQVSGGNTILDDNQSGFMTTAGSVTINSITATTLNFTFTFTREDGEVI
                     TGNYSGNYVDTSN"
     gene            complement(1293..1706)
                     /locus_tag="P5335_11930"
     CDS             complement(1293..1706)
                     /locus_tag="P5335_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRZ75635.1"
                     /translation="MPKIKDRVIVDTNLWISFLLSKDYGKLDKLFNAENLILLFSREL
                     LDEFVEVARRPKFKKYFSITDLNELLTEIHSRAEFIDVTSVVNLCRDEKDDFLLALAK
                     DGKADFLITGDNDLLELEKFEKTNILTMANYLQKK"
     gene            complement(1687..1911)
                     /locus_tag="P5335_11935"
     CDS             complement(1687..1911)
                     /locus_tag="P5335_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75636.1"
                     /translation="MKTYQIDILNPKAARLLQDLADLKLISIKETKKQDFMKIISKFR
                     KKASSNVPTLEEITKEVEMVRAERYAKNKR"
BASE COUNT          583 a          330 c          405 g          859 t
ORIGIN      
        1 tatttcgtta gttttggtaa atagtttttt tggtagatta agctctgatg tgttgtcctt
       61 tttaatggtc agaaagtagg ttgtttgcca attttgagtt ttttccgctt gtttacttgt
      121 tgcaatgtcc caagggggat aaactctaat tccacgtttt ccctctttgc cgttttttgt
      181 taggattcct tttgttgcca aaactgattt cgggaaaata aattgtccga agtggtctcc
      241 actttttgat gttattagta taaaatcgat atcgtctgaa aggtcaaatg gtgctgttac
      301 tccgtctttg tttcttttcc aaagggttac aaattgtcct ttttttgttg gtgtaatttt
      361 agaaacacgg tgttgaattt tcttcccgtt cagttcaaac gaacaagctc catagtctgc
      421 actttcatca ttctgtttga agtttgaaaa gtgaaaacca catttgtcat aaaccaattc
      481 tttcacaact gctaagtcgc tcggcagtgt gtttggtgtt gatagagtgt tttccattct
      541 catcttttct gttcgttctt gtttttggtg ttgctgctga atgttcgact ttaggtgtaa
      601 actttttttt aattgcttgt atccacatag tttccggagt agtttccggt tatcacttcg
      661 ccatcttctc tggtgaaagt gaaggtaaaa ttaagggtag ttgccgtaat ggaatttatg
      721 gtgactgagc cggcggtagt cataaagcca ctttggttat catctaaaat tgtatttccc
      781 cctgaaactt gaaaattgtt aaggtttgcg ttttcaagga tttggtaatc gggaaaattt
      841 gtgatggttc ccggttcgat attgaccgcc ggaaagtcga aaaaaacata atttataccc
      901 gtgctttgat ttgtttgaaa aggatcaaca tttgccaaaa ttatagaaat gtcggaaatg
      961 gtgttatcga tggtgttttc atcacttatg taggcatatt gagtggggta aaaatttccg
     1021 tttatattaa agccattgtt ttgtgcttga tttccatcat cctcctctga ggagcaagaa
     1081 aaagagatga gaataagtaa taatccggca attaaattta agtttttcat tcgttttgtt
     1141 tttaaaattg tttactgtgt ttttgtggct ttcattacag ggtaagtttc agcactaacc
     1201 tcacaaatat aattaaatct ttattttagt gttgttcgtt attgctattc aaaagacttg
     1261 aggataaagt ttgctgagtt tagcgaaata gtttatttct tttgaagata attagccatg
     1321 gttaagatat tcgttttttc aaatttttct aattcgagta aatcattgtc acctgttatt
     1381 aaaaaatctg cttttccatc tttagctaaa gcaagaagga aatcatcttt ttcatcacga
     1441 caaagattta ctacactagt tacgtcaata aattctgctc ttgaatgaat ttcagtcagt
     1501 agttcattta aatcggtaat tgaaaaatat tttttgaatt ttggtctacg tgcgacttca
     1561 acaaattcgt caagtaattc acgactaaaa agtaatatta aattttcagc gttgaaaagt
     1621 ttgtctagct ttccatagtc tttagaaagt aaaaaactaa tccaaagatt ggtgtctaca
     1681 ataactctat cttttatttt tggcataacg ctcagctcta accatttcta cttcctttgt
     1741 tatctcttct aaagtaggaa catttgaaga agctttttta cgaaattttg aaataatctt
     1801 cataaaatct tgctttttag tttctttaat tgagataagt ttcaagtcgg ctagatcttg
     1861 aagaagtctt gctgctttag ggtttagtat gtcaatctga tatgttttca tatttcaaat
     1921 ttagtaattt ttttcacaag atgattttct ttacgctttt tttagcatca tttctttgag
     1981 ttgcgttgtt taaaaaaatc ctgtttcgag gggaaacagg attttgggga atgcttcctc
     2041 ttattttttt tatgctgaac gttgttgttg ttccgcttgt ttaaggacga tgttgaaatc
     2101 gattcttgtg tttttggtga atactaagaa gactctttca gtgatgtagc catcgcgttt
     2161 gaaggttacg gggaaca
//
LOCUS       DAOTCW010000247         2147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_44751_length_2147_cov_38.242352, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000247 DAOTCW010000000
VERSION     DAOTCW010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44751_length_2147_cov_38.242352"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="P5335_11940"
     CDS             <1..437
                     /locus_tag="P5335_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HRZ75637.1"
                     /translation="DFYDRLKTVSKGYASFDYSPIGMRTSKLVKLDVLLNAQSVDALS
                     ALIHEDNAYNIGKKMCEKLRELIPRQQFDIPIQAAIGAKIIARETIKALRKDVTAKCY
                     GGDISRKRKLLEKQKKGKKRMRQVGNVEIPQEAFMAVLKLND"
     gene            790..1761
                     /locus_tag="P5335_11945"
     CDS             790..1761
                     /locus_tag="P5335_11945"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015026033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRZ75638.1"
                     /translation="MRNVFIKGTGSYAPENIVKNDFFESIGSSDEWIYSHLGIKERRI
                     STGETTSDLAYKAGLKAIENAGLTANEIDLILVATTTPDRQAPSCASMVQEKLQAFNA
                     VAFDVAAVCSGALFTTAVGVQFIKTGMYDTVLVIGADTFSNIIDWERRDAVFFGDGAG
                     AMVLSNTEEDKGFIDFILHTDGRGKEHFTIPAGGSEKPTSHETIDNRLHYFQMNGKEV
                     YKTAVKVVPECINEILIKNNISIDNIAFLIPHQPSIGILKEIAQKVNLPFEKVKTNMD
                     RYANTSGGTIPIVLDETHRNGEFKEGDLLLFAAVGAGWTWGTALYKW"
     gene            1795..>2147
                     /locus_tag="P5335_11950"
     CDS             1795..>2147
                     /locus_tag="P5335_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015026034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HRZ75639.1"
                     /translation="MFTGKTFLITGIADKNSLAMFVAKKIIENGGSVVCTGLGVSPFH
                     KNLSEKAAAFLNKNYQDFKDAVQEELGDAFTAIVDVTLDESIEHFAKTLQQKNIQLNG
                     FLHAIAMDKTIRLKEV"
BASE COUNT          749 a          300 c          417 g          681 t
ORIGIN      
        1 tcgattttta tgaccgattg aaaacggttt ccaaaggata tgcttcgttt gactattcgc
       61 caattggcat gagaacatct aaattagtaa aattagatgt gttgttgaat gctcaatcgg
      121 tcgatgcttt gtcggcattg attcacgaag acaatgcgta taacattggc aaaaaaatgt
      181 gtgaaaaatt gcgtgaactc atcccgagac aacaatttga cataccgatt caagcagcga
      241 ttggtgctaa aatcattgcc cgtgaaacaa taaaagcgtt acgaaaagat gttaccgcca
      301 aatgttacgg tggagatatt tcccgtaaaa gaaaactatt ggaaaaacag aaaaaaggta
      361 aaaagagaat gcgtcaagtt ggtaatgtag aaattcctca agaagcgttt atggcggtgt
      421 taaagttgaa tgattaagaa aggtgatgat tggctgagct actgagtatc tgagaaaata
      481 ataatgagaa cccaacgacg aaagttgttg ggttttttta tggcaatgat tgaatctttt
      541 ataatactat ttagctttcc atttgaattt ttttcaaatc ggaggtgttt tgataacgta
      601 ttttcatctt taaattatga tttgtgcttt ttttatatgc ctattttgac aatttcataa
      661 aaataaacac ttaaagttcc taaatcaaat gtggtgtttt aaaaacacta attttacctt
      721 attagatttt gattttattc attaatatat gaatttgaaa agaatttgaa agtttcaaaa
      781 caaacaaata tgagaaatgt ttttattaaa ggaaccggtt cctatgcccc tgaaaatata
      841 gttaaaaatg atttttttga aagcattggt tcttctgacg aatggattta tagtcattta
      901 ggaataaaag aaaggagaat ttctacgggt gaaaccacga gcgatttagc ctataaagcc
      961 ggattaaaag ctatagaaaa cgctgggtta acggctaatg aaattgattt aattttagtt
     1021 gccactacaa ctcctgaccg ccaagcccct tcgtgtgcga gtatggttca agaaaaactg
     1081 caggctttta atgctgttgc ttttgacgta gcagctgttt gttccggggc tttatttaca
     1141 acagctgttg gtgttcagtt cattaaaaca ggcatgtatg ataccgtttt agttattgga
     1201 gctgatactt tttctaacat tatagattgg gaaagaagag atgctgtatt ttttggtgat
     1261 ggtgccggag cgatggtgct ttcaaatact gaggaagaca aaggatttat cgatttcata
     1321 ttacacacag atggtcgtgg aaaagaacat tttacaatcc ctgcaggagg ttcagaaaag
     1381 ccaaccagtc atgaaactat tgacaatcgc ttacattatt ttcaaatgaa tggaaaagaa
     1441 gtttataaaa ccgctgtaaa ggttgtgcct gaatgtatca atgaaatttt gataaaaaac
     1501 aatatttcaa ttgataacat tgctttttta atcccacatc aacctagtat tggaatctta
     1561 aaagaaattg ctcaaaaagt gaatttgccc tttgaaaaag ttaaaacaaa tatggatcgt
     1621 tatgcgaata cttcaggtgg aacgattcca atcgtattag atgaaacgca tcgaaatggc
     1681 gaatttaaag aaggtgattt acttttattc gcagcagttg gtgccggatg gacatgggga
     1741 acagccttat ataaatggta aaaaatagag taataatatt aaaactgaaa taaaatgttt
     1801 acaggaaaaa cttttttaat aacaggaata gccgataaaa attcattagc catgtttgtg
     1861 gcaaaaaaga taatagaaaa tggtggttca gttgtttgca ccggattagg agtaagtccc
     1921 tttcataaaa acttatctga aaaggctgct gcttttttaa ataaaaatta tcaggatttt
     1981 aaagatgccg ttcaagaaga attaggagat gcttttacag ctattgtaga tgttacttta
     2041 gatgaaagca tagaacattt cgctaaaact ttacagcaaa aaaatattca attaaatggt
     2101 tttttacatg ctattgccat ggataaaact attcgtctca aagaagt
//
LOCUS       DAOTCW010000248         2135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_45157_length_2135_cov_52.783173, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000248 DAOTCW010000000
VERSION     DAOTCW010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45157_length_2135_cov_52.783173"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="P5335_11955"
     CDS             <1..570
                     /locus_tag="P5335_11955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ75640.1"
                     /translation="NASGINLTEFFNDWVYNQGYPSYTLTVANNAVNQALITVNQTQS
                     NSSVSFFEMPIQVRLNGAGGQTVDVVLNHTSNGQQFIVNVPFAVTSATFDPNKHIISK
                     NNSTTLGITTVDWSNVIQLLPNPTSNYLTIEVPETMQIENITVFNNLGQVVSSSNEKV
                     LMVSTLSDGVYYVNLTTSEGTFYKKFIKN"
     gene            650..1972
                     /gene="rseP"
                     /locus_tag="P5335_11960"
     CDS             650..1972
                     /gene="rseP"
                     /locus_tag="P5335_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009089975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRZ75641.1"
                     /translation="MEILIQIAQLILCLSFLVILHELGHFLPAKYFKTRIEKFYLFFN
                     PWFSLVKKKIGETEYGIGWLPMGGYVKIAGMIDESMDKEQLKQEPQPWEYRSKPAWQR
                     LIIIMGGVIVNFFLAWIIYIFLFLNHGDNFVDNSKITHGIAVDSVGKLLGLRNGDKIL
                     KVDGKPQAKFDQLPLEILFGDNITVEREGAEVTFDLSDEGKRDVIKRAGKNFLSAREE
                     IIIDSIVPGRPADIAGLKKGDKIVGLDGIHINYFDEFATVIQLHKNDSVDLDVIRNKE
                     PLVIRARVTEDSKLGFLNHFHTLEDAFVTKELSFSEAIPAGFNKTISTLTGQIKQFKI
                     IFNSKTEAYKQVSGPLRMFKIFKPEWDWTFFWSFTAMFSVWLAFLNVLPIPGLDGGHA
                     VFILIEMVTRKKPTEKTLEVAQTIGVVILLTLMALVFGNDIWHLITGK"
     gene            2061..2134
                     /locus_tag="P5335_11965"
     tRNA            2061..2134
                     /locus_tag="P5335_11965"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2095..2097,aa:Asn,seq:gtt)
BASE COUNT          713 a          354 c          381 g          687 t
ORIGIN      
        1 aatgcgtcag gaattaattt gactgaattt tttaatgatt gggtttacaa tcagggttat
       61 ccatcatata ccttaaccgt tgcaaacaac gctgtaaatc aagccttaat tacggtaaat
      121 caaacacaat ccaactcaag tgttagtttc tttgaaatgc caattcaggt aagattgaat
      181 ggtgccggcg gacaaacagt tgacgtcgtt ttaaatcaca cttcaaacgg acaacaattt
      241 atagttaatg tgccgttcgc agttaccagt gcaacttttg atccaaacaa acatataatt
      301 tctaaaaaca attcaactac attgggaatt acaacagtag attggtcaaa tgttattcaa
      361 ttactcccta acccgacttc aaattattta accattgagg ttccggaaac catgcagatt
      421 gaaaacatta ccgttttcaa caatttagga caagtagttt cttcatccaa cgaaaaagta
      481 cttatggttt caaccctttc cgatggcgtt tattatgtaa atttaaccac ctcggaagga
      541 actttttata aaaaatttat aaaaaattaa tcccaatcgt ttaaatctta ctgatttgta
      601 aagggaaaac cttacttttg taatcgcttt tgaattttaa aacacatcaa tggaaatact
      661 cattcaaata gcacaattaa ttttgtgctt atctttttta gtgatactac acgaattagg
      721 tcacttttta cccgctaaat attttaaaac acgtattgaa aaattctatt tatttttcaa
      781 cccttggttt tctttagtaa aaaagaaaat tggtgaaact gaatacggaa ttgggtggct
      841 tcctatgggt ggttatgtaa aaatagctgg tatgattgat gagagtatgg acaaagaaca
      901 actcaaacaa gagccacaac catgggaata cagaagtaaa ccggcctggc aacgtttaat
      961 catcatcatg ggcggtgtaa ttgttaactt ttttctggca tggataattt atatcttttt
     1021 atttctaaat catggagaca attttgtaga caattctaaa ataacccatg gtatcgctgt
     1081 tgattctgtc ggaaaactat tgggtcttag aaatggtgat aaaatactaa aagtggacgg
     1141 gaaaccacaa gctaaatttg atcaattacc tttagaaata cttttcggcg acaacattac
     1201 tgttgaaaga gaaggagcag aagttacttt tgatttatct gatgaaggaa aacgtgatgt
     1261 tatcaaaaga gccggaaaga acttcttatc tgctcgtgaa gaaatcatca ttgactcaat
     1321 tgtacctggt cgaccggcag atatagcagg attaaaaaaa ggagataaaa ttgttggctt
     1381 agatggaatt catatcaatt attttgatga atttgccacg gtgattcaat tacacaaaaa
     1441 cgatagtgtt gacttagatg taatacgaaa taaagaacct cttgtgatta gagctcgggt
     1501 aactgaagac tctaaacttg gttttttaaa tcattttcat acgctagaag atgcttttgt
     1561 aacaaaagag ttatcctttt ctgaagctat tccggctggg tttaataaaa ccatttctac
     1621 tttaacaggt caaattaaac aatttaaaat catttttaat tcaaaaacag aagcctacaa
     1681 acaagttagt ggtccgttac gaatgtttaa aattttcaaa ccggaatggg attggacatt
     1741 cttctggtca ttcaccgcaa tgttttctgt ttggctggcc tttttaaatg tgctgcctat
     1801 tccgggatta gacggtggtc atgccgtttt tatcctgatc gaaatggtta cacgcaaaaa
     1861 accaacagaa aaaaccctag aagttgctca aacaattgga gtggttattc tattgacatt
     1921 gatggcttta gtatttggta atgacatctg gcatttgata accggaaaat aaagatataa
     1981 aatgcatatt ttttgcgatt tttatttgga taaaacaatt ttcgctttat atttgcactc
     2041 gcaaaaaagg ttatatacct tcctccttag ctcagttggt tagagcatct gactgttaat
     2101 cagagggtcc ttggttcgag cccaagaggg ggagc
//
LOCUS       DAOTCW010000249         2135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_45158_length_2135_cov_33.304808, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000249 DAOTCW010000000
VERSION     DAOTCW010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45158_length_2135_cov_33.304808"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1796)
                     /locus_tag="P5335_11970"
     CDS             complement(<1..1796)
                     /locus_tag="P5335_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HRZ75642.1"
                     /translation="MNSGILIVILLLYLGLLFFIAQWAERKGNSKWTNNPYIYSLSLA
                     VYCTTWTYYGSIGVAANSGLNYIPIYLGPVIAFPAWIIILKKIIRISRVNKISSIADF
                     ISLRYGNSRFLGALVTLISLMAILPYVGLQLKAISETFHIVTNSPESNNVFLDTSTYV
                     ALVLALFASFYGTRYVDASEKRKGMVTAVAMESVLKLFFFIILGLYVTFYVFDGFDAI
                     YAKASLMENFEERNTIHGLEGGMNWMMLIILSFFSIFLLPRQFHMSIVENNREKHIQT
                     AIWLFPLYLLLFNLFVYPIAWGGNILFEGKDVNPDMFSLLIPQLFDNTFMTVLVFLGG
                     FSAAISMIVVSSITLSTMLSNNLLIPYGLLGSLQKDNQESNSERIVNIRRIGIFLLII
                     LAFIYYRYFIMDFSLVSVGMISFVIVAQLGPAFFGAIFWKRGSLNGAVAGIIVGMLIC
                     FYTIMLPFLLSSINPESIFFKEGFFGYTFLQPFQLFGLDYLDPIPHALFWSLLFNGLV
                     YLFVSVSFKGNYRERNYAEMFVDIDKYINNHENAFVWKGTAYVSDIQKVLKRFLGEER
                     TNRALSIFKMKYNIAPDNLMADARLVKFAENL"
     gene            complement(1789..1983)
                     /locus_tag="P5335_11975"
     CDS             complement(1789..1983)
                     /locus_tag="P5335_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75643.1"
                     /translation="MKKRHIQKFVILSFMLWMAFNFPILLLFDSADAILGIPIIYFYI
                     FSVWTLSIIVSFIFIKKYNE"
BASE COUNT          809 a          376 c          326 g          624 t
ORIGIN      
        1 aataaatttt cagcaaattt tactaaacgt gcatcagcca tcaaattatc gggagcaata
       61 ttgtatttca ttttgaagat ggaaagtgcc ctattggttc tttcttctcc taaaaaacgt
      121 tttaatactt tttgtatatc agatacatat gctgttcctt tccaaacaaa agcattttca
      181 tggttattga tgtatttatc aatatctaca aacatttcag catagttacg ctcacgataa
      241 tttcctttga aacttacaga gacaaataga tataccaatc cattgaacaa caaactccaa
      301 aaaagagcat gtggaatagg gtctaaataa tctaaaccga acaattgaaa gggttgtaaa
      361 aaagtatagc caaaaaaacc ttctttaaaa aaaatacttt ccggattgat tgaacttagt
      421 aaaaaaggaa gcataatggt gtaaaagcag atgagcattc ccacaattat tcctgcaaca
      481 gccccattta gcgaacctct tttccaaaaa atagctccaa aaaaggcagg tcctagttgg
      541 gctacaatta caaatgaaat cattccgacc gataccaatg aaaaatccat gataaaataa
      601 cggtagtaga taaaagctaa aataatcagc aaaaaaatac caattcttcg aatgtttaca
      661 atgcgttcgc tattgctttc ctgattgtct ttttgtaatg aacctaacaa accataggga
      721 attaagagat tgttactcaa catcgtagaa agtgttatcg aagatactac aatcatagat
      781 atggctgctg aaaatccacc tagaaagacc aaaactgtca taaacgtatt atcaaataat
      841 tgcggaatca aaagggaaaa catgtctgga tttacatctt taccttcaaa aagaatattt
      901 ccaccccacg ctatgggata aacaaataaa ttgaataaca aaaggtatag cggaaacaac
      961 caaatggctg tttgaatatg tttctctcga ttattttcta caatggacat atgaaattgt
     1021 cgtggtaata agaagataga aaaaaaggat aaaataatga gcatcatcca attcatgccc
     1081 ccttctaatc cgtgaatcgt gtttcgttct tcaaaatttt ccattagaga agctttggca
     1141 taaatggcat caaatccgtc aaaaacataa aatgtcacat ataatcctaa aatgataaag
     1201 aaaaataatt tgagcactga ttccattgca acagcagtaa ccattccttt tcttttttca
     1261 gaagcatcga cgtaacgagt tccataaaaa gaagcaaaaa gtgccaaaac caaggctaca
     1321 taagtcgaag tatccaaaaa tacattgttg gactccggtg aattggttac aatgtgaaat
     1381 gtttcagaaa tagctttgag ctgtaaaccc acataaggca aaatcgccat gagtgaaatt
     1441 aacgtcacta aagctcctag aaatcgactg tttccgtagc gaagggaaat aaaatcggca
     1501 atactggaga ttttattgac tctggaaatg cggattattt tcttcaaaat aattatccag
     1561 gccggaaaag caattaccgg tccgagatat attggaatgt aatttagtcc tgaattagca
     1621 gccacaccaa tactaccata atacgtccaa gtagtacaat aaacagcgag tgaaagtgaa
     1681 tagatataag gattattagt ccatttcgaa ttaccttttc gttctgccca ttgagcaata
     1741 aaaaaaagta aaccaagata caacagcaat ataactatta aaattccact attcattgta
     1801 tttttttatg aagataaatg atacgatgat tgacaatgtc caaactgaaa aaatataaaa
     1861 atagataatc ggtattccta aaatagcatc agcactgtcg aacaataaca gtattggaaa
     1921 gttaaatgcc atccataaca tgaaactgag aatcacaaat ttttgaatat gtcttttttt
     1981 cattttgatt attcaattta atcacaaact ctgctcttga aattcaaaag cagagattgt
     2041 aattttatct aaagataatt ctttttaatg tccggttgca tctcctgctc ccgatggaat
     2101 tcttatattt tcaacaatat cctgaacatt ttgtg
//
LOCUS       DAOTCW010000250         2122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_45580_length_2122_cov_38.826802, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000250 DAOTCW010000000
VERSION     DAOTCW010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45580_length_2122_cov_38.826802"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="P5335_11980"
     CDS             complement(<1..384)
                     /locus_tag="P5335_11980"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75644.1"
                     /translation="MKKVFSLILSFTLPILMFGQTKPVSKIATSETVYNVNQSDSEDA
                     QIVYKKGSKVPYSGIVFEKYENGKIASEIHYKNGIQDGLMTVWFENGNVAKKWNFSKG
                     KPEGLYTEWYENGQKEQEGTNRDGKL"
     gene            complement(590..>2122)
                     /locus_tag="P5335_11985"
     CDS             complement(590..>2122)
                     /locus_tag="P5335_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRZ75645.1"
                     /translation="LLLVCFVSTTTVAQLSSEKIDFLMTDALTKFKVAGASIAIVKDG
                     QVIHSKGYGVADYTSKKPVNENTNFQIASNTKAFTATALALLEEEGKLKWTDKVKDHI
                     AEFKMYNDYVTEHFNIQDLLTHRSGLGLGVGDLMFFPDGSDFTIKDVVTSFQHFKPTS
                     AFRTQFDYDNLLYIVAGEVIARASGMSYEQFVQKRIIAPLQMNNSFVGSLLKDKSNLA
                     SPHSSESGTIKIIDAYDIGMGSAAGGIYSNVADMSKWMITRLNKGKYGTDLKSSLFSL
                     ENHYEMWRIHTVTEANPNPRYNSHFSGYGLGWDLSDSKGNLRVEHTGGLPGMLSIVTM
                     YPDLNLGIVILTNTENGGSGLFYAVSNTISDSYLGLDDFGWTDKIVDRMNKYRGTGDE
                     VTAKVWAKVEASKNTPLKQEDYIGIYEDKWFGKVEVYRKDNQLWMKCHRSKKLNGPMA
                     FYNANTFAIKWEYQAMNCDAFAMFSLDETGKAQSIKMKGISPNIDFSFDFHDLDLQRV
                     KE"
BASE COUNT          655 a          442 c          315 g          710 t
ORIGIN      
        1 taattttccg tctcggttag taccttcctg ttctttttga ccgttttcat accattcggt
       61 ataaaggcct tctggtttcc ctttactaaa attccatttt ttagcaacat ttccattttc
      121 aaaccaaacg gtcattaaac catcttgaat accatttttg taatgtattt cactggcaat
      181 ctttccattt tcatactttt caaaaacaat accgctataa ggaactttgg aacctttttt
      241 ataaactatt tgcgcatctt ctgagtcact ttggttaaca ttatatacgg tttcgctagt
      301 tgctatttta gaaacgggtt ttgtttgacc aaacattaaa attggcaatg tgaatgataa
      361 gattaaacta aatacttttt tcattttgtt gtgttttttt atggttaata tttcgtttac
      421 ttataaatag tgttttataa ttgcttacac ttaaaaagca agagctatta agttagaaat
      481 tggtaaacat aaaactttat ataaaacact gaaaatcaaa agtacaaaac caaatttaaa
      541 tattacatta aaatctcaat ttcttaaaac cactattaac ggctggcttt tactccttaa
      601 ctcgctgtaa atccaaatca tgaaagtcaa aactaaaatc aatatttggc gaaatgcctt
      661 tcatttttat agattgtgct ttgcctgtct cgtctagtga aaacatcgca aaagcatcgc
      721 aattcatggc ttggtattcc catttaatgg caaaggtatt ggcattataa aaggccattg
      781 ggccatttaa ttttttcgaa cggtggcatt tcatccaaag ttgattgtct ttcctatata
      841 cttccacttt gccaaaccat ttatcttcat aaattccgat atagtcttcc tgttttagag
      901 gggtgttttt tgaagcttca acttttgccc aaaccttggc agttacttca tcaccagtac
      961 ctctatattt attcatccga tcaacaattt tgtctgtcca cccaaaatca tctaacccta
     1021 aataactgtc tgaaatggtg tttgaaacag cataaaaaag ccctgatcca ccattttcag
     1081 tattggttaa aatgacgatg cctaaattca aatcaggata catagttaca atcgaaagca
     1141 ttcccggcaa gccgccggtg tgctcaactc tcaaatttcc tttagaatct gataaatccc
     1201 aacctaagcc ataaccacta aaatgagaat tatatctcgg gttcgggttg gcttctgtaa
     1261 ccgtatgaat ccgccacatt tcatagtggt tctccaaaga aaataaagat gatttcaaat
     1321 cagttccata tttaccttta ttcaaacgcg taatcatcca tttactcata tccgcaacat
     1381 tcgaataaat accaccggca gcacttccca ttccaatgtc gtaagcatca attattttga
     1441 tagttcccga ttctgaggag tgtggactgg ccaggttact tttgtctttt aacaaactac
     1501 ccacaaatga attgttcatt tgtaaaggtg caataattct tttttgaaca aactgttcat
     1561 agctcatacc gctggctctg gcaattactt cacctgccac gatatacaat aaattgtcat
     1621 aatcaaattg agttctaaac gcagaggttg gtttaaagtg ttgaaagctc gtcacaacat
     1681 ccttaatcgt aaagtctgac ccgtcaggga aaaacatcaa atcaccaaca cctaacccaa
     1741 ggccgctacg gtgtgtcaac aaatcctgga tattaaaatg ctcggttaca taatcattat
     1801 acattttaaa ttcagcaata tggtctttca ccttatcagt ccacttcagt tttccctctt
     1861 cttctaaaag ggcaagggca gtggctgtaa aggctttcgt attcgacgca atctgaaaat
     1921 tggtgttttc attcaccggc tttttgctcg tataatctgc cacgccatac cctttggaat
     1981 gaatcacttg accatctttc acaatagcaa tagaagcacc tgccacctta aacttagtca
     2041 aggcatctgt cattaaaaaa tctatttttt cagaagatag ttgagcaacg gtggtagtcg
     2101 atacgaaaca aactaaaagc ag
//
LOCUS       DAOTCW010000251         2086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_46713_length_2086_cov_43.394387, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000251 DAOTCW010000000
VERSION     DAOTCW010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46713_length_2086_cov_43.394387"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="P5335_11990"
     CDS             <1..272
                     /locus_tag="P5335_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRZ75646.1"
                     /translation="DQIDYDMNSLDSSSSFYFSAQSHRMNEIMKTLTIVSVIFIPLTF
                     IVGVYGMNFENMPELKKPNGYFFTLAGMFILVMGMIFYFKRKKWF"
     gene            complement(273..857)
                     /locus_tag="P5335_11995"
     CDS             complement(273..857)
                     /locus_tag="P5335_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75647.1"
                     /translation="MKTLKYTLFFALATLLMAAECTKEDDEFYNAVYTTIPGLVSVET
                     QSNYQVNDVLWLNTNTFSRYLAEPNQSTPLDVYKTTNATKFSFLYTIEKKTGEDLWVS
                     VANGNNFIVESGSVSINNYITAFANYNQVTEKYEFRGGIKLTEPGEFRIGFFAGYNGE
                     NFDIISDSSTSSTFLTIATTANSLASGYYTFTVN"
     gene            complement(869..1438)
                     /locus_tag="P5335_12000"
     CDS             complement(869..1438)
                     /locus_tag="P5335_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75648.1"
                     /translation="MKKLFFIFLMLTQLSFAQNNNAFDLGKNEIKFDVVSLVALGKIH
                     VSYERFLKSDFSIGISGILNESNSLENDYVNGKSRTLEDYQIIPFVRYSLSKSQVRYY
                     FIEVFVSANSGTYHELERLIDENNNGYYLSVNKKYSDFAIGGAVGHKFYIKEKIGVDI
                     FVGMGKNLFSNGESPEIVSRVGINLGYRF"
     gene            complement(1517..>2086)
                     /locus_tag="P5335_12005"
     CDS             complement(1517..>2086)
                     /locus_tag="P5335_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRZ75649.1"
                     /translation="QDIVISKLLPFPNKSDIIKAIDFNNPRVRNFALTATTLHFKDIV
                     GQKEHRKMIQCFAVFKEIRNRWNYVNDPKGEEYIAAASESLIHFSGDCDDHAILMAAC
                     IRAVGGTPRLIHTGGHIYPEILVGKKSELEAAVYLIKEVLFITESKNKEIHYHIDERG
                     QIWLNLDYTARYPGGPFMSEEILGALTLN"
BASE COUNT          690 a          364 c          320 g          712 t
ORIGIN      
        1 tagatcaaat agattacgat atgaactcac ttgacagttc atccagtttt tatttttcgg
       61 cccaaagtca ccgcatgaat gaaatcatga aaacactaac aatcgtttcg gttatattta
      121 ttcccttaac ttttattgtt ggagtatacg gaatgaactt tgaaaacatg cctgaattaa
      181 agaaacccaa tggttatttc ttcactttag ccggcatgtt tattttagtg atgggaatga
      241 tattttattt taaacgtaaa aaatggttct aattagttaa cggtaaaagt ataatagccc
      301 gaagccaagc tatttgctgt agttgcgata gttaaaaagg ttgagctggt tgagctatca
      361 gaaatgatat caaaattttc accattatag cctgcaaaaa atccaatacg aaattctcct
      421 ggctcggtta gctttattcc gcctctgaat tcatatttct cggtcacttg attgtaattg
      481 gcaaaggcag ttatataatt attgatggac accgatccgg attcaacaat aaagttgttc
      541 ccattggcaa cacttaccca aaggtcttca ccggttttct tttcaatcgt atataaaaag
      601 ctgaatttag ttgcatttgt tgttttataa acatctaacg gggtcgattg atttggctca
      661 gctaaatacc gagagaaagt attggtattc aaccataata catcatttac ctgataattg
      721 gattgtgttt ctaccgatac taaaccggga atggttgtat aaacggcatt ataaaattca
      781 tcatcttctt tggtacattc ggcagccatt aataaggtgg ccaaagcaaa aaataaagta
      841 tatttcagtg ttttcatagt gctatttatt aaaatcggta acctaaatta attccaacgc
      901 gagatacaat ttcagggctt tcaccattgc tgaaaaggtt ttttcccata cctacaaaaa
      961 tatcaactcc gattttttct ttgatataga atttatgacc tacagcacct ccgatggcaa
     1021 aatcggaata ttttttgttg acagagagat aataaccatt attgttctcg tcaattaatc
     1081 gttctaattc atgataagtc ccactgtttg cagacacaaa aacttctata aaataataac
     1141 gaacttggct tttggataga gaataacgaa caaaagggat tatttgataa tcttccagcg
     1201 ttctgctttt cccgttgaca taatcgtttt ctaatgaatt actttcattt aaaattccgc
     1261 ttattccaat tgaaaaatcg gattttaaaa aacgttcata actcacatga attttaccta
     1321 aagcaactaa ggaaacaaca tcaaatttga tttcattttt tcctaaatca aacgcattgt
     1381 tgttttgtgc aaaagatagt tgagtaagca ttaaaaatat aaaaaacagc tttttcattt
     1441 tttggtttta taaagtatgt aaatatataa aaaggaagtt ttattcgata ataattaacc
     1501 aaaatctcaa ttaaaattaa ttcaacgtca atgcacccag aatttcttct gacataaaag
     1561 gtcctccggg ataacgagcg gtataatcta agtttaacca aatttgccct ctttcatcaa
     1621 tatgataatg gatttctttg tttttacttt ctgtaataaa taaaacttct ttaattaaat
     1681 aaacggcagc ttccaattca ctttttttac ccactaaaat ttcaggataa atgtgtcctc
     1741 cggtatgaat caaccttgga gtgccaccaa cagctcggat gcaagcggcc attaagatgg
     1801 cgtggtcatc acaatcacct gaaaaatgta ttaaactttc tgaagcggct gcaatatatt
     1861 cttctccttt tggatcattt acataattcc aacgatttct gatttcttta aatacagcaa
     1921 aacactgaat cattttacga tgttcttttt ggccaacaat atctttgaaa tgtagagttg
     1981 tggctgtcaa cgcaaaattt ctaacccttg ggttattaaa atcaattgct tttataatat
     2041 ccgatttatt tggaaagggc aacagttttg aaataacaat atcctg
//
LOCUS       DAOTCW010000252         2073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_47171_length_2073_cov_28.512884, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000252 DAOTCW010000000
VERSION     DAOTCW010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47171_length_2073_cov_28.512884"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="P5335_12010"
     CDS             <1..1415
                     /locus_tag="P5335_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75650.1"
                     /translation="ARQPFNAFPVNSNNTNIYVVDINTNQFWTASLNTLSTSLKEQLQ
                     ATNFNFYQDGTTLYVLGGYAFSTTADDHVTFNKLTAIDVPNLINAVISGSSILSYFKQ
                     MANENFAVTGGHLAKIDDTFYLIGGHRFDGRYNPMNNPTFVQTYTNAIRKFSIDNSGT
                     QLSISNYESISDAVHLHRRDYNLLPQIFPDGSKGYTISSGVFQINVDLPFLYPVDVKS
                     DGYFPQTAFNQYLSNYHSAVSALYDATENRMHNLFFGGISQYYYNGTNLIQDNTVPFV
                     NTISRMTRFADGSLQEYQLPETMPALKGASAEFIPNESLPHYDNEVIKLNDITVDEFV
                     IGHIVGGITSPSTSPFTNNQTSSTSANATIYEVKLIKNTPLGIHEINGKNPFSMSVSP
                     NPTTSGKVRINFSMPYVTTVDYFVSSLDGKIISDGEISEAKQGENAMNFELDNVSNQV
                     VIITFIFDNKFYVSQKVVVN"
     gene            1569..>2073
                     /locus_tag="P5335_12015"
     CDS             1569..>2073
                     /locus_tag="P5335_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HRZ75651.1"
                     /translation="MKYLILVIVFISFFSCQQKKEDADYSNKKTNQVAIENAQSGKKL
                     LETHCYVCHNPTIPDSLGRIAPPMVAIKARYLMNYITKEEFVNHIVQFVENPTEDNAI
                     MYGAVNRFKVMPKQVFPEGVVEKIANYMYEYQIEEPVWFKEHWESSGNGQGRRKGKGE
                     WIQPGKKL"
BASE COUNT          710 a          309 c          366 g          688 t
ORIGIN      
        1 atgctcgaca accatttaat gcttttcctg ttaatagtaa taataccaac atttatgtgg
       61 tcgacatcaa cactaatcag ttttggacag cttccttgaa taccttgtct acttctttaa
      121 aagaacaatt gcaagcaaca aattttaatt tttatcaaga cggaacaaca ttatatgttt
      181 tgggagggta tgctttttcg actactgcag acgatcatgt tacgtttaat aaattaacgg
      241 caattgatgt tccaaatttg ataaatgcgg taattagtgg gtcttccatt ttgagttatt
      301 ttaaacaaat ggctaatgaa aattttgccg tcactggcgg tcatctggca aaaattgatg
      361 atacgtttta tttgattggc ggacatcgtt ttgacggaag gtataatcct atgaataacc
      421 caacttttgt tcaaacttat acgaatgcta ttcgaaaatt ttctatcgac aattccggaa
      481 cacaattgag tatttctaat tacgaaagca tctctgatgc tgtacatttg caccgacgtg
      541 attataattt attacctcag atttttccgg atggttcaaa agggtatacc atttcgtccg
      601 gagtttttca aatcaatgtt gatttacctt ttttatatcc ggttgatgtt aagtctgacg
      661 gatattttcc tcaaacagct ttcaatcaat atttaagtaa ttatcatagt gctgtgtcag
      721 ccttgtatga tgcaactgag aatagaatgc ataatttgtt ttttggagga ataagccagt
      781 attattataa cgggactaat ttaattcagg acaatacagt gccttttgtc aatacaatca
      841 gtaggatgac tcgttttgct gatggttctt tacaagaata ccaattaccc gaaacgatgc
      901 ctgctttaaa aggagcgagt gctgaattta ttcctaatga aagtttgcca cattatgaca
      961 atgaagttat caaattgaat gatattacag tggatgaatt cgtcattgga catattgtgg
     1021 gaggaatcac gagtcctagc acgagtccat ttacaaataa tcagacaagt tcaacttctg
     1081 caaatgccac aatttatgaa gtgaaattaa ttaaaaatac acccttggga atacatgaaa
     1141 taaatggaaa aaatccattt tcgatgtctg tttcacctaa tccaacaaca agcggaaaag
     1201 tcagaattaa tttttcaatg ccatatgtaa caacagttga ttattttgtt tcttctttag
     1261 atggtaaaat tatttctgat ggagaaatta gtgaggctaa acaaggtgaa aatgcaatga
     1321 attttgaatt ggataatgtt tcaaatcagg tagtcattat tacttttatt tttgacaata
     1381 aattttatgt gtctcaaaag gtagttgtga attaaaaaaa tctcaatcat attattcgca
     1441 ggaaacttat tgattcttaa gttttctgtg atactttatt actttataaa atagtgaact
     1501 taaagtaaca ttagttgcaa attcattcta tctgaaaagg taactttaca ttttaaatta
     1561 tatttatcat gaaatattta attttagtca tagtttttat aagttttttt tcttgccaac
     1621 aaaagaaaga agatgcggat tattctaaca agaaaacaaa tcaagtagcc attgaaaatg
     1681 cacaatcagg aaaaaaatta ctagaaacgc attgctacgt atgtcataat cctactattc
     1741 ctgatagttt aggccgaatt gctccaccga tggttgcaat aaaagcccgt tatttaatga
     1801 attatataac aaaggaagaa tttgtcaatc acattgttca atttgtagaa aatccaacag
     1861 aagataatgc gattatgtat ggagcagtca atcgttttaa agtgatgccc aaacaagttt
     1921 ttccggaagg agtagtggaa aagattgcta attatatgta tgagtaccaa atagaagaac
     1981 cggtttggtt caaagaacat tgggaaagta gcggaaatgg acaaggaaga agaaagggaa
     2041 aaggcgaatg gattcagccc ggtaaaaaat tgg
//
LOCUS       DAOTCW010000253         2064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_47488_length_2064_cov_33.317073, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000253 DAOTCW010000000
VERSION     DAOTCW010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47488_length_2064_cov_33.317073"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /gene="hutI"
                     /locus_tag="P5335_12020"
     CDS             complement(<1..1126)
                     /gene="hutI"
                     /locus_tag="P5335_12020"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006261132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRZ75652.1"
                     /translation="MKTMLINIKELIQVRETGILKVSGAEMAVLPTIKNAYLLFENDI
                     ISDYGSMENYPNQTFDELIDATGRVVLPTWCDSHTHLVYAGNRVQEFVDRINGLSYEE
                     IANRGGGILNSAQKLNETAENEIYEQSKIRLEEVMKLGTGAIEIKSGYGLTVEGEIKM
                     LRVIRRLAENYPIKIKATFLGAHAFPKEYKENHADYIDLIINEMLPKIAAEKLADYID
                     AFLETGYFSVEETERIMIAGKKHGLIPKIHVNQFTAIGGIAVCVKHNALTVDHLEVVT
                     YEDIEVLKNAETMAVALPSCSYFISIPYTPARKIIDAGLPLALATDYNPGTTPSGNMN
                     FVVATACIKMKMTPEEAVNAATINGAYAMGVSNTHGSITKG"
     gene            1205..>2064
                     /locus_tag="P5335_12025"
     CDS             1205..>2064
                     /locus_tag="P5335_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRZ75653.1"
                     /translation="MKRLKYKKGRKVVSNYFEYTGHHKEYPVEMQLFVYNSESYTETK
                     NLVFSQVEIKSLLASDDVKWLNIHGLHDVELIKKIVESIEIDSYIAGDILNVAKRTRM
                     EELGDVLFFSIKSILPEENETNVHVEQISFLLKNNLLVSFQEKRGDFFTHIRERIKTN
                     SGIVCKKKNDFLLYLMLDAVMENFYITIEKYEELIEQLQIESKVNEKQDVLVRIEKCR
                     EDLNYLKRSIIPLKDSLYNLKSIQDDDDFNGIEKSNFTFFARLHQKSLELLDQIDYDM
                     NSLDSSSSFY"
BASE COUNT          694 a          342 c          321 g          707 t
ORIGIN      
        1 taccttttgt aatactcccg tgtgtatttg aaactcccat agcataagct ccattaatcg
       61 ttgcagcatt aacggcttct tcgggtgtca tcttcatttt aatacaagcc gttgccacca
      121 caaaattcat gttccctgaa ggggtggtgc ccgggttata atctgttgct aacgctaatg
      181 gtaaacccgc atcaattatt tttctagccg gcgtataagg aatgctgata aaataactgc
      241 aggaaggcaa tgcaacagcc atggtttcag cattttttaa tacttcgatg tcttcatacg
      301 tcactacttc taaatgatct acggttaaag cattatgttt cacacaaact gcaataccac
      361 caattgctgt aaattgatta acatgaattt ttggaattaa cccatgtttt tttccggcaa
      421 tcattattcg ttcggtttct tctactgaaa aataacctgt ttctaaaaaa gcatcaatgt
      481 aatcagctaa tttttcagct gcaattttag ggagcatttc attaatgatt aaatcaatat
      541 aatcggcatg attttcttta tattcttttg gaaaagcatg agctcctaaa aaagtagctt
      601 ttattttgat gggataattt tctgctaaac gtcggattac ccgtagcatt ttgatttcac
      661 cttccactgt caatccataa ccggatttga tttcgatggc tccggttccc aatttcataa
      721 cttcttctaa acgaattttt gattgctcat aaatttcatt ttcagccgtt tcattcaatt
      781 tttgagcgga atttaaaatt ccacctccgc gattggcaat ttcttcgtaa ctcaatccgt
      841 taattctatc cacaaattcc tgaactcgat tgccggcata aactagatgc gtgtggctat
      901 cgcaccatgt aggcaaaact actcttccgg ttgcatcaat tagctcatca aaagtctgat
      961 tggggtaatt ttccatactt ccgtaatctg aaatgatatc attttcaaaa agaagatagg
     1021 cattttttat agttggcaac actgccattt ctgcacctga aacttttaga attcccgttt
     1081 ctcttacctg aataagttct tttatattaa ttaacatcgt tttcataaag taaaagcata
     1141 ataatttacc gtaaaaatag aatgaaataa ttaaaacact taattttata aaaaaataaa
     1201 acgtgtgaaa cgattaaaat ataaaaaagg tcgaaaagta gtatctaatt attttgaata
     1261 tacaggacat cacaaagaat atcctgtgga aatgcaatta tttgtatata attcagaatc
     1321 ttatacagaa actaaaaatt tggttttcag tcaggttgag attaaatccc ttttagcctc
     1381 tgatgatgtt aaatggctaa atatccatgg tttacatgat gttgaactta taaaaaaaat
     1441 tgtagaatcg attgaaattg acagttatat tgccggtgat attttaaatg ttgccaaaag
     1501 aacccgtatg gaagaattgg gcgatgtttt gtttttcagt ataaaatcta ttttaccgga
     1561 agagaatgaa acaaacgtac atgttgaaca aatcagtttt ttattaaaaa ataatttatt
     1621 ggtttccttt caagaaaaaa gaggtgattt ttttacacac atcagagaac gaataaaaac
     1681 aaattccgga attgtttgca aaaagaaaaa tgatttttta ttgtatttaa tgttagatgc
     1741 agtgatggaa aacttttaca ttaccattga aaaatatgaa gaattaattg aacagctcca
     1801 aattgaatca aaagtaaatg aaaaacaaga tgttttagtt cggattgaaa aatgccgtga
     1861 agatttaaac tatttaaaac gttccatcat tccgttaaaa gattcgttgt acaatttgaa
     1921 atctattcag gacgatgatg attttaatgg aattgaaaag tcaaatttta ctttttttgc
     1981 cagattacac caaaaaagtc tggaacttct agatcaaata gattacgata tgaactcact
     2041 tgacagttca tccagttttt attt
//
LOCUS       DAOTCW010000254         2060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_47637_length_2060_cov_29.400499, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000254 DAOTCW010000000
VERSION     DAOTCW010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47637_length_2060_cov_29.400499"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="P5335_12030"
     CDS             complement(<1..964)
                     /locus_tag="P5335_12030"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRZ75654.1"
                     /translation="MTSIKNTFIKDLLAGLTVSFAALALGAAFGTMSGRGAFAGMVGA
                     AIIPIITSLIGGTRLQASGPTGPMTAVTALLVAKAYDMFPDKLLAEQFITLTIIMSGF
                     FMILGGLFRLGKLIKYVPNVIVIGFMNGIAVLIWWDQILKIGGLGGKKIMEGVPLVNV
                     ILGIFTLILIFLIPIIIRKLRVPDNIRSYFSAVLISIVIGTLMSNLIGLDVEKVKLGA
                     TFNSLNEYGEMLSRFFPGLEIVQKEYILIALPLALQLMLLGYLDSLLTSLVIDRIVGE
                     QTKENKELVAQGIANGVSGLLQGIPGAQATIRSVLLIKEQAQTRL"
     gene            complement(978..>2060)
                     /locus_tag="P5335_12035"
     CDS             complement(978..>2060)
                     /locus_tag="P5335_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRZ75655.1"
                     /translation="HLGVFLMLRTYPFWEHQTSMRIAIGLMGLTTSIAASFMAKVQSS
                     VKSQIAYSSISQIGIIFIEIALGLETLALIHFAGNAFLRTYQLLVSPSVVSYLIREQF
                     YNFQLKTNKNFFRFPKKIENTIYILALKEFNLEGFLNFVLWKPLKVIGKTLDFLNIKR
                     VYYFFVPLFVLGFLAYKFKLDLPEQMVNILPEIFAFIGLVFVFKSFSERKSPFLAWIL
                     IVLNHFWIALAIVFNDKVSIYEIVFYLSGIIIAGTIGYLALLKLKQIEMRILINQYLG
                     HVYEHPKFAFFFLLATLGITGFPITTTFIGEDLIFSHIESSQLFLAFFISSSFVVSGI
                     AGIRIYARLFLGPHVKTYHELPYKSS"
BASE COUNT          748 a          382 c          286 g          644 t
ORIGIN      
        1 ccaatcgagt ttgtgcctgt tctttaatta ataaaaccga cctgattgtt gcttgagcac
       61 caggaatccc ttgcagtaat cctgaaactc cgtttgctat tccttgagca acaagttctt
      121 tattctcttt tgtttgttca cctacaattc ggtctataac taaagaagtt agcaaactat
      181 ccagatatcc taataacatg agttgtaaag ctagtggcaa tgcaatcaaa atatattctt
      241 tttgtactat ttccagaccc ggaaaaaacc ttgacaacat ttcaccatat tcatttaatg
      301 aattaaaggt tgctcctaat ttcacttttt ctacatcgag accaataaga ttcgacataa
      361 gcgtcccgat aacaatagaa atcaataccg cagaaaaata cgacctaata ttatccggaa
      421 ctcttaattt tcttattatg attggtatta aaaaaattaa aatcaaagta aatattccta
      481 aaattacatt aacaagagga acgccttcca ttattttttt tcctcctagt cctcctatct
      541 ttaatatttg atcccaccaa atcaaaactg caattccatt cataaagcca attacaatta
      601 cattgggaac atattttatt aattttccaa gacggaaaag acctcctagg atcataaaaa
      661 aaccactcat aataattgtt aatgtgataa attgttctgc taataattta tccggaaaca
      721 tatcatatgc ttttgctact aaaagtgctg taacagctgt catgggtccg gttggacctg
      781 aagcttgtaa cctagttcct ccaattaaag atgtaattat gggtattatg gcagctccta
      841 ccattcctgc aaaagcacct ctccctgaca ttgtcccaaa agcagcccct aaagctaagg
      901 ctgcaaaact taccgttaat ccggctaata aatcttttat aaatgtgttc tttatagatg
      961 tcataaatta atttgtgtta agatgatttg taaggcaact catgatatgt tttcacatga
     1021 ggacctaaaa ataaacgggc ataaattctt attccggcga ttccggaaac aacaaagctg
     1081 gatgaaataa aaaaggctaa aaaaagttga ctactttcga tatggctaaa aatcaaatct
     1141 tctcctataa aagttgttgt gattggaaat cctgtaattc ccaaagttgc aagcaagaag
     1201 aaaaaagcaa attttggatg ttcataaaca tgccctaaat attgatttat taaaattctc
     1261 atttctattt gcttcaattt taatagtgct aaataaccta ttgttccggc aattatgatt
     1321 cctgataaat aaaatacaat ttcataaata ctaactttat cattgaaaac tatagctaat
     1381 gcaatccaaa agtgatttaa cacaatgaga atccatgcta aaaatggact tttacgttca
     1441 gaaaatgatt taaaaacaaa aaccaatcct ataaaagcaa aaatttcagg caagatattg
     1501 accatttgtt cgggtaaatc caacttaaac ttataagcta aaaaccctag cacaaacagg
     1561 ggtacaaaaa agtaatatac tctcttgata tttaaaaaat ctaatgtttt gcctattact
     1621 tttaatggtt tccaaagaac aaaattcaag aaaccctcca gattaaattc tttcaatgcc
     1681 aaaatataaa tagtgttttc tatctttttc ggaaaccgga aaaaattctt gttcgtttta
     1741 agttgaaaat tataaaactg ctctctaatc aaataactca caacggaagg agaaaccaat
     1801 aattgatatg ttcgtaagaa agcatttccg gcaaagtgaa tcagtgctaa agtttccaaa
     1861 ccaagagcaa tttcaatgaa aataattccg atttgagaaa tggaactata agcaatctga
     1921 ctttttactg aagattgcac ctttgccata aatgatgccg caatacttgt tgttaaaccc
     1981 attaaaccaa ttgctattcg catgcttgtt tgatgttccc aaaaaggata ggtacgcaac
     2041 atgagaaaaa ctcccaaatg
//
LOCUS       DAOTCW010000255         2048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_48058_length_2048_cov_24.987456, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000255 DAOTCW010000000
VERSION     DAOTCW010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48058_length_2048_cov_24.987456"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="P5335_12040"
     CDS             <1..656
                     /locus_tag="P5335_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HRZ75656.1"
                     /translation="YNAPFLFETNVGAGLPIIDTLKHLIASGDRIHKIQAVLSGSLNF
                     IFNNFSETYNFHDVVKEAGVQGFTEPDPKIDLSGVDVARKILILIRESGYQMEMEDIE
                     NNSFLPKECMETTNNEDFFKSLIKHASHFEKLLKEAKEKDCRLKFVAKFDNGKASVGL
                     QFIPKESPFYNLEGKDNIVEFFTDRYIDQPLIIKGAGAGAAVTASGIFADVIRIGNV"
     gene            750..1670
                     /locus_tag="P5335_12045"
     CDS             750..1670
                     /locus_tag="P5335_12045"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRZ75657.1"
                     /translation="MNEIKIFCPATVANLSCGFDVLGLCLEGIGDEMIIRKVKEKGLK
                     ITKITGADLPLETNKNVAGVAGLALLNHLDLEFGFEIEIHKKIKAGSGIGSSAASAAG
                     IVVGINEVLGNPLHRKDLIPFAMEGEFLASGSYHADNVSPAILGGFTLVRGYEPLEVI
                     KIKSPKDLYVTVIHPHIEVKTSEARNILPKEILLKTAITQWGNLGGLISGLYTEDYGL
                     IGRCLHDVVAEPYRKNLIPEFENIKKTSLENGALGCGISGSGPSIYALSKGLKTAKNV
                     ADGIKKIYSKTGIGFNIYLSKVCEEGVKVM"
     gene            complement(1744..>2048)
                     /locus_tag="P5335_12050"
     CDS             complement(1744..>2048)
                     /locus_tag="P5335_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRZ75658.1"
                     /translation="FFETEKQTEGPLHMVFNPPYGERLNIDMERFYREIGDTFKQSYP
                     NTQAWFITANLEALKFVGLKPSRKIKLFNGKLEARLVKYDIYEGSKRTKFQNREEE"
BASE COUNT          686 a          322 c          396 g          644 t
ORIGIN      
        1 aatacaatgc tccctttttg tttgaaacca atgtaggtgc cggtttaccg attatagaca
       61 ccttaaaaca tttaattgct tcgggtgatc gaattcataa aattcaagca gttttatctg
      121 gaagtttgaa ctttattttt aataatttca gtgaaacgta taattttcac gatgtggtga
      181 aagaagccgg agtgcaaggt ttcacagaac ctgatccaaa aattgatcta agtggagtag
      241 atgtcgctcg taaaattttg attcttattc gcgaaagcgg ttatcaaatg gaaatggaag
      301 acattgaaaa caattcattc ttaccaaaag aatgtatgga aacaactaac aatgaagatt
      361 tttttaaatc cttaatcaaa catgctagcc attttgaaaa attattgaaa gaagccaaag
      421 aaaaagactg tcgtttaaaa tttgtggcta aatttgataa tggaaaagct agcgttggtt
      481 tgcaatttat tccaaaagaa agtccatttt acaacctcga aggaaaagac aatatagttg
      541 aattttttac cgaccgttat attgatcaac cgttaatcat caaaggtgcc ggtgccggtg
      601 ccgccgtaac tgcatcaggt atttttgcgg atgtgattcg gattggaaat gtataaatta
      661 gtttaaagtt gtttaaggtt gtcggtttca gctaattcaa caattttaaa ctatttaaac
      721 aatgttaaac atacttaaat ccatactaaa tgaacgaaat aaaaatattc tgccccgcca
      781 ctgtcgccaa cttgtcctgt ggatttgatg tgttgggctt atgtttggaa ggtattggag
      841 atgaaatgat tatccgaaaa gtcaaagaaa aaggactgaa aatcaccaaa ataacgggag
      901 ccgatttgcc tttggaaacc aataaaaatg tagccggtgt tgccggatta gcattattaa
      961 atcacttaga tttagaattt ggttttgaaa ttgaaatcca caaaaaaatc aaagccggaa
     1021 gcgggatagg aagcagtgcc gccagtgctg ccggaattgt ggtaggtatt aatgaagtat
     1081 taggcaatcc attgcatcgg aaagatttaa ttccatttgc aatggaaggc gaatttttag
     1141 ccagcggaag ttatcatgca gataatgttt ctcctgcaat tttaggcgga tttaccttag
     1201 ttcgaggtta tgaaccgtta gaagtaatta aaattaaaag tccaaaggat ttgtatgtca
     1261 ccgtcataca tccgcatatc gaagtaaaaa cttctgaagc aagaaacatt ttacccaaag
     1321 aaattctgtt aaaaactgcc atcactcaat ggggaaattt aggcggtttg attagtggtt
     1381 tatatactga agattacgga ttgataggtc gatgcttgca tgacgttgtt gctgaacctt
     1441 atcgaaaaaa cttaattcca gagtttgaaa acataaagaa aacatcttta gaaaatggtg
     1501 ctttaggttg tggaatttca ggttccggac cttctattta tgctttgagc aaaggtttaa
     1561 aaactgctaa aaatgtggct gacggaatta aaaaaatcta ttcaaaaaca ggaattggat
     1621 ttaatatata tttgtcaaaa gtttgtgagg aaggggtgaa agtaatgtaa aattactttt
     1681 tcatgtttta aaagaggaaa ctcacaaagt gattcctgtt tcgtaatgat aagattacag
     1741 aggctactct tcttccctat tctgaaactt cgtccgttta ctaccttcgt aaatatcata
     1801 tttcaccaat cgagcttcta attttccatt gaataatttg attttacgcg aaggttttaa
     1861 gcccacaaat ttcaatgctt ccaaattggc cgtaataaac catgcttgtg tgttgggata
     1921 actttgttta aacgtatcgc caatttctct ataaaatcgt tccatatcaa tattcaaacg
     1981 ttcaccatag ggcggattga aaaccatatg aagcggacct tcggtttgtt tttccgtttc
     2041 gaaaaagt
//
LOCUS       DAOTCW010000256         1947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_52048_length_1947_cov_42.308140, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000256 DAOTCW010000000
VERSION     DAOTCW010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52048_length_1947_cov_42.308140"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="P5335_12055"
     CDS             complement(<1..586)
                     /locus_tag="P5335_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HRZ75659.1"
                     /translation="MEECISVFDMLKIGVGPSSSHTLGPWRAAERFLAEIRTEHLLTN
                     AIRVKVDLYGSLSLTGKGHATDYAVMLGLSGQDPETIPVAAIDGIIQAIKTDKKIKLG
                     NEIWIDFDVENDIVFNKNFLPFHANGLTFTIFTKEKTYSDTFYSIGGGFVVTERRSNA
                     KHNQEIKCSFPYPIQKADELLAYCNSLNKSISEIV"
     gene            755..>1947
                     /locus_tag="P5335_12060"
     CDS             755..>1947
                     /locus_tag="P5335_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRZ75660.1"
                     /translation="MNRIYSFLFLFIGCFCFSQPKSPSEFLPNYGKQVTYYHQVEAYF
                     KQLVQNSDWIQYQKYGETNQERNLNAYYISTPENLKNLEEIRKNHLNAIGLYPTKSTA
                     VKDKTIVWLSFNVHGNEIGAVESAMNVAYELVNPTNKDTKNWLQDIVVILDPCLNPDG
                     FSRYANWLRDISGKKTHPGTTDREHMEPWPGGRQNHYLYDLNRDWAWQTQIETQLRMD
                     LYHQWMPMVHADVHEMGYNEPYFFPPAAEPYHEQIALFQRDFHKKIGELTSKKFDKEG
                     WMYYSGERFDLFYPSYGDTYPCYNGAVGMTYEQGGIGAGRAVVMKNGELLTIQDRINH
                     HTKALLAVVELSFVQKESLTKEFRAYFKDNRTKPKGKYQTYIIKNSAKNLELTNLLNR
                     NRIESS"
BASE COUNT          676 a          307 c          343 g          621 t
ORIGIN      
        1 aaacaatttc agaaatagat ttatttaatg aattgcaata cgctaataat tcatctgctt
       61 tttgaattgg atagggaaac gaacatttta tttcctgatt atgtttagcg ttacttcttc
      121 gttcagtcac tacaaagcca ccaccaatgg aataaaaagt gtcgctatat gttttttctt
      181 ttgtaaaaat tgtaaaagtt aatccattgg catgaaaagg gagaaagttt ttattgaaaa
      241 caatgtcatt ttcaacatca aaatcaatcc aaatttcatt acctaatttg atttttttat
      301 cggttttgat agcttgaatt attccatcaa tagctgcaac cggaattgtt tccggatctt
      361 gcccacttaa accaagcatc acggcataat cagtagcgtg accttttccg gttaacgaaa
      421 gggaaccata taagtcaact ttaacccgaa tagcattggt taaaagatgc tctgttctaa
      481 tttctgccag aaatcgttct gctgctcgcc aaggtccaag tgtatgagaa cttgaaggac
      541 ctactccgat tttaagcata tcaaaaacag aaatacattc ttccataact atatcgtttt
      601 agttaggcaa atatagtatt ttgtttaaag gtatttcact attaaatctc actaacgatt
      661 tagtttaaca aaaagagtga ttattagtaa atgaaatgtt aatccaatgt tcggttattc
      721 caaaatccat atatttgaat tctaaatttt aaagatgaat cggatttaca gttttttatt
      781 cctttttata ggttgttttt gtttttctca acctaaatct ccttctgaat ttttaccgaa
      841 ttatggaaag caagttacgt attatcatca ggtagaagcc tattttaagc aactagtcca
      901 aaattctgat tggattcaat atcaaaaata tggagaaacc aatcaggaac gaaacttgaa
      961 tgcgtattat atttcaacac ctgaaaattt aaaaaattta gaagaaattc gaaaaaatca
     1021 tttgaatgcc attgggttat atccaacaaa atcaacagct gttaaagata aaaccattgt
     1081 ttggttgagt tttaacgtac atggaaatga aattggggca gtcgaaagtg cgatgaatgt
     1141 tgcctatgaa ttggtgaacc caacaaataa agatactaaa aattggttgc aggatatagt
     1201 agtgattctc gacccgtgtt tgaatccgga tggtttttcg cgatatgcca attggcttcg
     1261 tgatatttcc ggaaaaaaaa cacatcccgg aacaacagat agagaacaca tggaaccatg
     1321 gccgggaggt agacaaaatc attatctcta tgatttgaat cgtgattggg cttggcaaac
     1381 ccaaattgaa actcaattga gaatggattt atatcatcag tggatgccaa tggttcatgc
     1441 cgatgttcat gaaatgggct ataatgagcc ttattttttt ccaccggctg ccgaacctta
     1501 tcatgaacaa attgctcttt tccaacgtga ttttcataaa aaaatagggg agttaacctc
     1561 taaaaaattt gacaaagagg gttggatgta ttattcaggt gaacgatttg atttgtttta
     1621 tccaagttat ggcgacactt atccttgtta taatggtgca gtaggaatga cttatgagca
     1681 gggaggaata ggtgccggga gagcagtggt aatgaaaaat ggagaattat taacgattca
     1741 agatcgcatt aaccatcaca ccaaagcact tttagctgtt gtggagttat cttttgttca
     1801 aaaagaaagt ttgacaaaag aattcagagc ttattttaaa gacaacagaa caaaacccaa
     1861 aggaaaatat caaacctata ttataaaaaa tagtgcaaag aatttagaac ttactaattt
     1921 attaaataga aatcgaattg aaagttc
//
LOCUS       DAOTCW010000257         1940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_52335_length_1940_cov_29.095491, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000257 DAOTCW010000000
VERSION     DAOTCW010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52335_length_1940_cov_29.095491"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1901)
                     /locus_tag="P5335_12065"
     CDS             complement(<1..1901)
                     /locus_tag="P5335_12065"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRZ75661.1"
                     /translation="MKHFSLFFLFVFLFFGIEIAFGQKKSVQAIRITENIVVDGKLNE
                     SSWEKAPLAKDFVMYEPDNGASEKHEKRSEVKIIYNDNGVYIGATLYDNETTKIEKEI
                     TQRDVFGTADRFGIFLNGFNDGQQDFRFFVTAAGVQLDCLATENGEDFSWDAIWKSDI
                     SITDFGWVVEMEIPYAALRFSKNDKQIWGLNIFREIRRDRHNYVWSHIDRAKGSVINQ
                     AGTLEGIENIKTPTRLFFIPYTSGYIESNDKTTNSTGKIGMDIKYGINDSFTLDAILV
                     PDFGQTAFDNVELNLGPFEQQFNENRPFFTEGTDLFSKGDLLYSRRIGGPPSTYPVTN
                     ENETVTDYPNTVDLINAVKISGRTSDGLGIGFLNAATAKTYAKITNNETNESREELVE
                     PHTNYNILVFDQRFNQNSSVSFINTNVTRFGDFRDANVTGIVYNLNTKGNKFNVSGDV
                     KMSSIYDDEKPNGHTAYLNLGKTFGRYRYSVASKYVSKDFDNNDLGINFVTNYHNVYA
                     NFNYRIVKPTKTYNSFQLNSNYYLEIENTTGKLQENWVNVNVNSSTLKNDYIGYGFLV
                     SPFVTYNFYEPRVPGRYLAFPATGNAWIFFSSNYNRRFAIDVEPSVRFFNQKDRINYS
                     LYASPRYRF"
BASE COUNT          654 a          339 c          281 g          666 t
ORIGIN      
        1 ttaaatcggt atcttggact ggcatataaa ctataattaa ttctatcttt ttgattaaaa
       61 aaacgaacgg aaggctcaac gtcgatagca aatcgtctat tgtaatttga agagaaaaaa
      121 atccaggcat ttcccgtggc aggaaaagct aaatacctcc caggaacacg aggttcataa
      181 aaattataag taacaaatgg acttactaaa aagccataac cgatatagtc gttttttaaa
      241 gtggaggagt ttacattgac attaacccaa ttttcctgca attttccggt tgtgttttca
      301 atttctaaat aataattaga attaagttga aaagaattgt acgttttagt tggctttact
      361 attcggtaat taaaattagc ataaacatta tgataattgg tcacaaaatt gattcctaaa
      421 tcattattgt caaaatcttt tgaaacatat tttgaagcaa cagaataacg atatcggcca
      481 aatgttttac ctaaatttaa ataagccgta tgaccatttg gtttttcatc atcataaata
      541 ctactcattt ttacatctcc ggaaacatta aatttatttc cctttgtgtt taaattatag
      601 acaattccgg taacgtttgc atcacgaaaa tcaccaaatc ttgtcacatt tgtgttgata
      661 aaagaaactg acgaattctg attaaaacgt tgatcaaaca ctaaaatatt ataattagtg
      721 tggggttcaa cgagttcttc tctggattca ttggtttcgt tgttggttat tttagcatat
      781 gtttttgctg tggcggcatt taaaaaacca attccaagcc catctgaagt tctacctgat
      841 atttttactg cattaattaa gtcaactgta tttggataat cagttacggt ttcattttca
      901 tttgttacgg gataagtaga tggtggacca ccaattcttc tcgaatagag caaatcacct
      961 tttgaaaaaa gatctgttcc ttcagtaaaa aaaggtctat tctcgttaaa ttgttgttca
     1021 aaaggcccta aattcagttc tacattatca aaagcagttt gaccaaaatc aggcactaaa
     1081 atggcatcta aagtaaagga atcattaatt ccgtatttaa tatccattcc gatttttccg
     1141 gtgctatttg tagttttatc gtttgattca atataaccgg aagtataagg gataaaaaat
     1201 aatcgggtag gagttttaat attttcaata ccttctaacg ttcctgcttg gttgataaca
     1261 gaacctttcg ctctatcaat atgactccaa acataattat gtctgtctct tcgaatttct
     1321 ctaaaaatat taagtcccca aatttgttta tcatttttag aaaatcgtaa agcggcataa
     1381 ggtatttcca tttcaacaac ccaaccaaaa tcagtaatgg aaatgtcact tttccaaatg
     1441 gcatcccaag aaaaatcttc cccattttca gttgccaaac agtccaattg aactcctgca
     1501 gctgtaacaa aaaaacgaaa atcctgttgg ccgtcgttaa atccatttag aaatatgcca
     1561 aatctatccg ctgttccaaa aacatctctt tgggttattt ctttttcaat tttagttgtt
     1621 tcattatcat aaagcgttgc accaatataa acaccgttat cgttatatat aattttcact
     1681 tcacttcttt tttcatgttt ttctgaagca ccattatccg gttcatacat cacaaaatct
     1741 tttgcaaggg gagctttttc ccacgatgat tcgtttaatt taccgtctac aactatattt
     1801 tctgttattc ggatagcctg aacagatttt ttttgaccaa aagcaatttc aattccaaaa
     1861 aagagaaaaa caaataagaa aaataggcta aaatgcttca taaagtaaat aatgttcaaa
     1921 attagataaa gcttttccaa
//
LOCUS       DAOTCW010000258         1900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_54095_length_1900_cov_39.130623, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000258 DAOTCW010000000
VERSION     DAOTCW010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54095_length_1900_cov_39.130623"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="P5335_12070"
     CDS             <1..758
                     /locus_tag="P5335_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRZ75662.1"
                     /translation="TLKEFQDFNKKFYIPNNAVLVVAGQFEPAQAKEWIQKYFGVIQK
                     GTPITRTKAEETPITQTLKAKYEDPNIQIPMAVASYRTPSMKTRDARVLDMISTILSE
                     GKSSRLYKKIVDEKKMALQVGAFNFSQEDYGLYILYGLPMGGFTPETLIAEIDEEIVR
                     LQTELISEKEFQKLQNIFESQYVDSNASVEGIAGNLATYYLLYGDVNLINSEIDIYRS
                     ITREEIRDIAKKYLNPNQRLLLDYAPKSDKAQN"
     gene            769..>1900
                     /locus_tag="P5335_12075"
     CDS             769..>1900
                     /locus_tag="P5335_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRZ75663.1"
                     /translation="MKKIAILATSLFLTLTMQAQDRPQPKPGPAPKINIGKPQTFELP
                     NGLKVLVVENNKLPRVSYNLSIDNAPYSEGNKKGVSDMTSSMLGNGTKTISKNDFNEE
                     VDFLGASINFSSDGAFASGLSKYSKRIMELMADGALNPVFTQEEFEKEKAKLIEGLKA
                     DEKSVPSIARRVERVLAYGKNHPRGEYLSEETINTISLNDVIQNYTSYFTPENAYLVI
                     VGDVKFAEVKTTVEKLFGSWKKATAPLVNYSDPKDVQYTQINFVDASNAVQSEISAVH
                     ITKLKMTDKDYFAAIVANQILGGDFNSYLNMNLREAHGWTYGARSGLRGNKYVGNFSA
                     STQVRNAVTDSAVVEILKEIKRIRTEKVSEETLKNVKAGYIGK"
BASE COUNT          705 a          305 c          338 g          552 t
ORIGIN      
        1 caacgcttaa agagtttcag gattttaata aaaaattcta tattccaaac aatgcagtgc
       61 tggttgttgc cggtcaattt gaacctgcac aggcaaaaga atggattcaa aaatattttg
      121 gtgttattca aaaaggaaca cctataacca gaactaaagc ggaagaaacc ccaattactc
      181 aaacacttaa agcaaaatat gaagacccaa atattcaaat tccaatggcg gtagcttcct
      241 acagaactcc ttccatgaaa acacgtgatg caagggtatt ggatatgatt tcaactattt
      301 tgtcagaagg caaatcttcg cgtttgtaca aaaaaattgt ggatgagaaa aaaatggcct
      361 tacaagtagg agcttttaat tttagtcaag aagattacgg tttgtatatt ttatacggat
      421 tacccatggg cggatttact ccggaaactt taattgctga aattgacgaa gaaattgttc
      481 gccttcaaac tgaattaatt tctgaaaaag aatttcaaaa actacaaaac atctttgaaa
      541 gtcaatatgt agacagcaat gcttctgtgg aaggtattgc ggggaattta gctacttact
      601 atttattgta cggtgatgtg aatttgataa attctgaaat agacatttat cgttctatta
      661 cccgtgaaga aattagagat attgctaaaa aatacctcaa tccaaatcaa cgtttacttt
      721 tggactacgc tcctaaatct gataaagctc aaaattaaga tataaatcat gaaaaaaata
      781 gccatattag caacgagctt gtttttaact ttaactatgc aagcacaaga cagacctcaa
      841 ccaaaaccag gaccggctcc taaaattaat attggaaaac ctcaaacttt tgaattacca
      901 aacggattaa aagtgttagt tgttgaaaac aataaattac caagagtttc ttacaatctt
      961 tccattgaca acgctccata ttcagaagga aacaaaaaag gtgtttctga tatgacaagt
     1021 agcatgttag gtaatggaac caaaaccatt tctaaaaatg actttaatga agaagttgat
     1081 tttttaggtg cttctatcaa tttcagctcc gatggtgctt ttgctagcgg attatctaag
     1141 tattcaaaaa gaatcatgga attaatggct gatggagcat taaaccctgt ttttacgcaa
     1201 gaagaatttg aaaaagaaaa agctaaatta attgaagggt taaaagccga tgaaaaaagt
     1261 gttccctcca ttgccagaag agtagaacgc gttttagctt atggaaaaaa tcatcctaga
     1321 ggcgaatatt taagcgaaga aaccatcaat actatttcac taaacgatgt tattcaaaat
     1381 tatacttctt attttacacc tgaaaatgcc tacttagtta ttgtgggcga tgtaaaattt
     1441 gctgaagtaa aaactacagt agaaaaatta tttggtagtt ggaaaaaagc aaccgctcct
     1501 ctggtaaatt attctgaccc gaaagatgtt caatacacgc aaattaattt tgtagatgct
     1561 tctaatgcgg ttcaatctga aatttcggct gttcacatta cgaaattaaa aatgaccgac
     1621 aaagattatt ttgccgcaat tgtagcaaat caaattttag gtggagattt caacagttac
     1681 ttgaacatga acttaagaga ggctcacggt tggacttatg gtgcccgttc aggcttaaga
     1741 ggaaataaat atgttggaaa cttttctgct tctacgcaag tgagaaatgc agtaactgac
     1801 agtgccgtag ttgaaatttt aaaagaaata aaacgcatcc gaactgaaaa agtatctgaa
     1861 gaaacattaa aaaatgtaaa agcaggatac attggaaaat
//
LOCUS       DAOTCW010000259         1893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_54393_length_1893_cov_55.615343, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000259 DAOTCW010000000
VERSION     DAOTCW010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54393_length_1893_cov_55.615343"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..967
                     /locus_tag="P5335_12080"
     CDS             182..967
                     /locus_tag="P5335_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HRZ75664.1"
                     /translation="MALNVIRRKELYEIMPEGVIATHKWLMENNLTRHAIDNLVKSKQ
                     LESISKGVYVRNISKISWQSVVFSLQSIVKTDLVIGGLTALEMQGLSHYVSLSENKTV
                     HLFGNDVLPEWVTNLHLNVKFVRHTTHCLFAKNSEENKQLHPFTLERDWDNEGRKLII
                     STPERAYLEVLADVPQKITFEHADQLMQGLTTLSPRNLQKTLEGCQNVKVKRLFFWFA
                     DRQNYVWLGKINRESITLGAGNRMLIKGGKLDPKYKITVPEWL"
     gene            958..1890
                     /locus_tag="P5335_12085"
     CDS             958..1890
                     /locus_tag="P5335_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRZ75665.1"
                     /translation="MAIDANNRYRKQVQLLVRVLPLIDTEKCFALKGGTAINLFYRAL
                     PRLSVDIDLLYIPSDDRETALINIRTALSRISKLIQQKIPGTKVQNTHDQSDALRLIV
                     SQEEIRIKVELSPVIRGTVFSEVRMEVVEEVEREFGYVEMQVASLPDLYAGKLCAALD
                     RQHPRDLFDVKFLLENEGLTDDLRKTFLLFLISHQRPMSELLAPNRKDISEIYETEFK
                     QMAEIDVPLEELEEARENLIQQINSQMTDNEKKFLLSFKNKTPDWNLLEMENVAAIAN
                     LPSVLWKMINLEKMPAQKHKEAYDKLQNILFPTV"
BASE COUNT          726 a          287 c          340 g          540 t
ORIGIN      
        1 gtattcctaa agccggtact acagtttgct tcattccgct aattttattc aaaaagaaaa
       61 atttaaataa atttaaaata acggcgatta tcgccgttga aatcaattta tgtaaattaa
      121 ataatttata ttacctttgt ataacggcga ttaacaccgc aataagctaa acttttaaat
      181 tatggcttta aatgtaatac gaagaaaaga gttatatgaa ataatgcccg agggtgtaat
      241 agccactcat aaatggctta tggaaaataa tttaacgcgt cacgctatag ataatttggt
      301 taaaagtaag caactggaat ctatcagcaa aggggtttat gttagaaata tcagcaaaat
      361 atcctggcaa tcagtggttt tctcattgca atcaatagta aagaccgatt tggttatagg
      421 tggtttaaca gcacttgaaa tgcagggttt atcacattac gtatcacttt ctgaaaataa
      481 aacagtgcat ttattcggaa atgatgtact gccggaatgg gtgacaaatt tacacttgaa
      541 tgtgaaattt gttagacata ctacccattg tttatttgct aaaaattcag aagaaaacaa
      601 acaattacat ccttttacgt tagaaaggga ttgggataat gaaggtagaa aattaatcat
      661 atcaactcct gaaagagcgt atcttgaagt attagcagac gtaccacaaa aaataacttt
      721 tgaacacgca gatcaattaa tgcaaggcct aacaactttg tcgcctagaa accttcaaaa
      781 aacattagaa gggtgtcaaa atgtaaaagt aaaacggctt ttcttttggt ttgcagaccg
      841 tcaaaattat gtttggttag gtaaaataaa tagagaaagc ataacattag gtgctggaaa
      901 cagaatgctc ataaaaggcg ggaaattaga tcctaaatat aaaataacag ttccggaatg
      961 gctatagacg caaacaatag ataccgaaaa caagtgcaac ttttagtgcg tgttttacca
     1021 ttgatagata ccgaaaaatg ttttgcatta aaaggaggta cggcaataaa tctattttac
     1081 agagcattgc ctcgactttc agttgacatc gatttgctat acattcctag tgatgatcga
     1141 gaaacagcat tgataaatat tagaactgct ttatcaagaa taagcaaatt gatacaacaa
     1201 aaaataccgg gcactaaagt acaaaacaca cacgatcaaa gcgatgcact acgattaatt
     1261 gtaagtcaag aggagattcg catcaaagta gaattatctc cggtgatcag aggaactgtt
     1321 ttttcagaag ttagaatgga agtggtcgaa gaagtagaaa gagaatttgg atatgtagaa
     1381 atgcaagtag catcacttcc tgatttatat gcgggtaaat tatgtgcggc tttagacaga
     1441 caacatccaa gagatttatt tgatgtaaaa tttcttctcg aaaacgaagg actaacggat
     1501 gatttacgaa aaacattctt actatttctt ataagccatc aacgaccaat gtcagaactg
     1561 ttagctccca atcgtaaaga tattagcgaa atttatgaaa cagagttcaa gcaaatggct
     1621 gaaatagatg tgcctttgga agaattagaa gaagctagag aaaatttaat tcaacaaatc
     1681 aattctcaaa tgacagacaa tgaaaagaaa tttcttttgt cttttaaaaa caaaacacct
     1741 gattggaatt tactggaaat ggaaaatgta gccgcaattg ctaatcttcc ttcagtactt
     1801 tggaaaatga taaatcttga aaaaatgcct gctcaaaaac acaaagaagc gtatgataag
     1861 ttacaaaata tattatttcc aactgtataa aaa
//
LOCUS       DAOTCW010000260         1887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_54666_length_1887_cov_35.904476, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000260 DAOTCW010000000
VERSION     DAOTCW010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54666_length_1887_cov_35.904476"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..613
                     /locus_tag="P5335_12090"
     CDS             248..613
                     /locus_tag="P5335_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRZ75666.1"
                     /translation="MTMKKTLVIGASVNPERYSFMAIHSLLKKEHSVVAIGQKLGEVA
                     GVKIQTKQIPFKNIHTVTFYLNPKGQRDYYNYIIGLQPKRVIFNPGTENPEFYALLGI
                     NNIKVEVACTLVLLSTNQY"
     gene            complement(590..>1887)
                     /locus_tag="P5335_12095"
     CDS             complement(590..>1887)
                     /locus_tag="P5335_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRZ75667.1"
                     /translation="GMIGASLSGVTFISVPGKVEASQFVYFQLVLGYIVGYFIIGTVL
                     LPLYYKLNLTSIYSYLGDRFGRYSYKTGAWFFIISRTVGSNLRLMLVADVLQTLVFDK
                     LNFPYWATVSITILLIWVYTNKSGIKTIIWTDTLQTFFMLVSVIVCIVIVSQDLGLNI
                     STISGFIAESDFSKTFFFDDISSPNYFWKQFLSGAFIAIVMTGLDQDMMQKNLTCRSI
                     KDAQKNMFWFTIVLTFVNFLFLVLGLMFTEYALKYNIDAHKDQLFPILANEYLGVSVA
                     FFFILGLIAAAYSSADSALTSLTTSFSIDILEIEKKYDTIKQVVIRKQIHKLFVFISI
                     IVILCFKYFVEDASVIDKVLKFAGFTYGPLLGLYSFGLFTKWNVKDKLVPIVAITAVI
                     SSIILNLFSESWFGFAFGFEILIVNGILTFIGLVLIRRK"
BASE COUNT          724 a          307 c          291 g          565 t
ORIGIN      
        1 gataatatct aatcggggtg aaccaattcc gtttagttta atattgtcaa ttaaaccaaa
       61 aggttttcca accaattcat taatcgtttg ggtgattttt tttttgttat atgaagtatt
      121 gactagcatt ttttctttaa aaataataaa caattatgac caattaaaac gattaataaa
      181 ggtttaagaa ttgttgaggt ttctattttt aaaatataaa tcaaaaaaat cgtactttta
      241 caaaaaaatt acaatgaaaa aaaccttagt aattggagct tctgtaaatc ccgaacgcta
      301 ttcatttatg gcaatacaca gtttattgaa aaaagaacat tcggtagttg ctattggaca
      361 aaaacttgga gaagttgcag gggtgaaaat tcaaactaaa cagattcctt ttaaaaatat
      421 ccataccgta acgttctact taaatccaaa aggacaacgc gattactata attatatcat
      481 tggtttgcaa cccaaaagag tgatttttaa tccaggaaca gaaaacccgg agttttatgc
      541 tttgttggga atcaataata taaaggtaga agtagcctgt acgttagttt tactttctac
      601 gaatcaatac taagccaata aaagtcagta ttccgttcac aattaatatt tcaaacccga
      661 aagcaaatcc aaaccacgat tcactgaaaa gatttaaaat aattgaactg attacagcag
      721 taattgcaac aatgggaact aatttatctt tcacattcca tttagtaaac aatccaaagc
      781 tgtaaagccc taataatggt ccgtaagtga aaccggcaaa tttcagtact ttatcaatga
      841 cgctggcatc ttcgacaaaa tacttaaaac aaagaataac aataattgag ataaagacaa
      901 ataatttgtg gatttgttta cgaatcacaa cttgtttgat ggtgtcatat ttcttttcga
      961 tttctagaat atcaatacta aatgaagttg tcaaagaggt caaggcacta tctgcactcg
     1021 aataagccgc agcaattaat cccaaaatga agaaaaaggc aacactaaca cccaaatatt
     1081 catttgctaa aatggggaaa agttgatctt tgtgtgcatc tatattgtat ttgagtgcat
     1141 attctgtaaa cattaatcct aaaactaaaa acagaaaatt cacgaaagtt agaactatgg
     1201 taaaccaaaa catgtttttt tgagcatctt ttatacttcg gcaagtgagg tttttttgca
     1261 tcatgtcttg gtctagtccg gtcatgacaa tcgcaataaa agctccggat aaaaactgtt
     1321 tccagaaata atttggagaa cttatgtcat caaaaaagaa agttttggaa aaatcacttt
     1381 ctgcaatgaa acctgaaatg gttgagatat ttaaacctaa atcctgcgat actatcacaa
     1441 tgcaaactat cacagaaact aacataaaaa aggtttgtaa agtatctgtc caaatgatgg
     1501 ttttgatacc cgatttattg gtgtaaaccc atattagtaa aatcgtaata gaaacggtag
     1561 cccaataggg gaaatttaat ttatcaaaaa ccaatgtttg caatacatct gccactaaca
     1621 ttaaacgaag atttgatcca acagttcgtg aaattataaa aaaccaagct cctgttttat
     1681 aagaataacg gccaaatcgg tcacctaaat aagaataaat ggaggttaag tttaacttat
     1741 aatataaagg caataataca gttccgataa tgaaataacc aacaatatag cccaatacca
     1801 attgaaaata gacaaattgt gatgcttcta cttttccggg tacggagata aaagttacac
     1861 ccgagagcga agcaccaatc attccaa
//
LOCUS       DAOTCW010000261         1881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_54930_length_1881_cov_38.413472, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000261 DAOTCW010000000
VERSION     DAOTCW010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54930_length_1881_cov_38.413472"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1881)
                     /gene="recG"
                     /locus_tag="P5335_12100"
     CDS             complement(<1..>1881)
                     /gene="recG"
                     /locus_tag="P5335_12100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRZ75668.1"
                     /translation="YGDLLTFFPNRYIDRTRYYKINELHSRSLGSNAEVQIIGKIIHL
                     KTVEQKKGRGRLVATFVDETGEMELVWFQGQKWVRESLKLNTPYVAFGKVTAFSAMFN
                     MAHPELELLEEHKESLRSALQPVYPSTEKLTQRGISNRTINKMMQQLFVETQARFSET
                     LPNYLADELKFIPKNAALFNIHFPKSQDLLARAQFRLKFEELFFIQLQLITKNLIRKN
                     KIKGYPFEKVGTYFNDFYQHHLPFELTNAQKKVLKEIRNDLGSNAQMNRLLQGDVGSG
                     KTIVALMCMLIALDNGFQSCLMAPTEILATQHFNGLSELAKELNIKIKLLTGSTKTAE
                     RKIIHEQLESGELHIIIGTHALLEDKVQFNNLGLAIIDEQHRFGVEQRSKLWKKNDIP
                     PHVLVMTATPIPRTLAMSLYGDLDISVIDELPPGRKPIQTVHRFDSNRLKVWKFLKDE
                     IAKGRQIYIVYPLINESEKMDYKDLMDGYESISRDFPLPHYSVSIVHGQMKAAAKEEE
                     MRRFALGKTNIMVATTVIEVGVNVPNASVMVIESAERFGLSQLHQLRGRVGRGAEQSY
                     CILMTSHKLSDDSKTRLETMCKTNDGFEIAEVDLKLRGPGDLMGTQQSGVLNLQIADL
                     VKD"
BASE COUNT          570 a          377 c          292 g          642 t
ORIGIN      
        1 gtccttaacc aaatcagcaa tttgtaaatt caaaacgcca ctttgttgtg ttcccataag
       61 atcacccggg ccacgaagtt ttaaatctac ttcagcgatt tcaaaaccgt cgttggtttt
      121 acacatcgtt tctaatcttg ttttactgtc atcactcaat ttgtggctcg tcattaaaat
      181 gcaataactt tgctccgcac cacgaccaac gcgacctcga agttgatgta gttgtgaaag
      241 gccaaaacgc tcggcacttt caatcaccat cacactggca ttgggtacat ttacgccaac
      301 ttcaataacc gttgtggcta ccataatatt ggttttacct aaagcaaaac gacgcatttc
      361 ttcctcttta gcggctgctt tcatctgtcc gtgaacaatg gaaacgctgt aatgaggcaa
      421 tggaaaatca cgtgaaatac tttcataacc atccattaaa tctttgtaat ccattttctc
      481 actttcgtta atcaacggat atacaatata gatttgacgg cctttggcaa tttcatcttt
      541 taaaaatttc caaactttta atcgattgga atcaaatcga tgaacggttt gaataggttt
      601 tctaccagga ggtaactcat caatcaccga aatatctaaa tcgccatata aactcatcgc
      661 taatgttctc ggaattgggg tagccgtcat caccaaaacg tgaggaggaa tatcattttt
      721 cttccataat ttagatcgtt gttcaacacc aaatcgatgt tgttcatcaa taatggctaa
      781 tcctaaattg ttaaactgaa ctttatcttc caacaaagca tgggtgccaa ttataatatg
      841 caattcacca ctttctaatt gctcatgaat gattttacgt tcggcagttt ttgtggaacc
      901 ggtgagtaat tttattttga tattcaactc tttagctaat tcacttaatc cattaaaatg
      961 ttgtgtagct aatatttccg ttggtgccat caaacaactt tggaatccgt tatccaaggc
     1021 tatcagcatg cacattaacg caacaattgt ttttcctgaa ccaacatcgc cttgtaataa
     1081 acgattcatt tgggcattac tccctaaatc atttcgaatt tctttcaata cttttttttg
     1141 agcatttgtt aattcaaaag gtaaatgatg ctggtaaaaa tcattaaaat aagtacccac
     1201 tttttcaaat ggatatcctt tgattttatt ttttcgaata aggtttttag taattaattg
     1261 cagttgaata aaaaacaact cttcaaactt taatcgaaat tgtgctctcg ccaataaatc
     1321 ctgacttttt ggaaaatgaa tgttgaataa agcagcattt ttcggaataa attttaattc
     1381 atctgctaaa tagtttggca acgtttctga aaaacgagct tgtgtttcca caaacaattg
     1441 ttgcatcatt ttatttattg ttcgattaga aatgcctctt tgagtcagtt tttcagttga
     1501 tgggtaaacc ggttgcaaag ctgaacgcag actttcttta tgttcttcca ataattctaa
     1561 ttcaggatga gccatgttaa acattgctga aaaagcggta acttttccaa aagcaacata
     1621 aggtgtattg agttttaaac tttcacgaac ccatttctgc ccctgaaacc aaaccaattc
     1681 catttcgccg gtttcatcaa caaaagtggc gactaatctt cctcttcctt ttttttgctc
     1741 aacggtttta aggtgaataa tctttccaat aatttgcact tctgcattac tgcctaaact
     1801 tcgcgaatgc aattcattga ttttataata tcgtgtacgg tcgatatagc gatttgggaa
     1861 aaaagttaac aaatctccat a
//
LOCUS       DAOTCW010000262         1871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_55382_length_1871_cov_34.699339, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000262 DAOTCW010000000
VERSION     DAOTCW010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55382_length_1871_cov_34.699339"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="P5335_12105"
     CDS             complement(<1..124)
                     /locus_tag="P5335_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRZ75669.1"
                     /translation="MQIPTIVGAGLIVIGAGLGIGKIGGSAMDAIARQPEASGKI"
     gene            complement(159..1304)
                     /gene="atpB"
                     /locus_tag="P5335_12110"
     CDS             complement(159..1304)
                     /gene="atpB"
                     /locus_tag="P5335_12110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRZ75670.1"
                     /translation="MVISKKPLHFIVALLVSVLPLLTFANSSDSTVVHTEAVHTEVEA
                     QHSEEPKDLKTEIKEFINHHLLDSYDFHLFSWEDDSKVEHHVGFPLPVILVDNGVQFF
                     MSSKFHHGESVAESNGNFYKLYHGKIYKTDAQGTISYDADHHATNPKPLDFSITKNVF
                     MMFLVGVLMLWLFSSLAKSYAKNGGIATGAGRFFEPIILYIRDDIAIPNIGHKNYKKY
                     MSYLLTIFFFIWFLNMFGLTPLGVNVTGNIAITAGLAILTYLITTFTANKNYWGHIFW
                     MPGVPTPMKIILAPIELLGTIIKPFSLMIRLYANIVAGHVVLMSIIGLMFIFKNWLGS
                     SLSFFLAFALSLLEILVAALQAYIFTMLSALYFGAANEEHHHEEGHH"
     gene            complement(1423..1821)
                     /locus_tag="P5335_12115"
     CDS             complement(1423..1821)
                     /locus_tag="P5335_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75671.1"
                     /translation="MISIKKLWDIYKFLLVVVPIFIIHIIIFQESTLKNAAQDFYYSI
                     PFLYFIYIVFSKIILFVIIKVSEKNFDSTGMTFLVCTSIKMAVSYFILRPILNSSSEN
                     TIEKVNFFAVFILFLAIETLITIRLLNKNQ"
BASE COUNT          677 a          298 c          295 g          601 t
ORIGIN      
        1 gaatttttcc agaagcttct ggttgacgag cgatagcgtc cattgctgaa ccaccgattt
       61 taccaatacc taaaccagca ccgataacga ttaatcctgc tcctacaatt gttggaattt
      121 gcataatgta tttattaatt aattaaacat tcaatttatt aatgatgccc ctcctcatgg
      181 tggtgctctt cattggccgc tccaaaatag agagccgata acatggtgaa aatataggct
      241 tgcaatgcag caaccaaaat ttccaataac gataaggcaa aagctaagaa gaaggataaa
      301 ctacttccta accaattttt gaaaataaac atcaaaccaa tgatactcat taatactacg
      361 tgtccagcaa caatgtttgc ataaagacgt atcattaatg aaaatggttt aatgattgtt
      421 cctaataact caattggagc taaaataatt ttcattggag ttggaacacc cggcatccag
      481 aaaatatgtc cccaataatt tttattagca gtaaatgttg taatcaaata cgttaaaata
      541 gcaagtcctg cagtaatggc aatattacct gtaacgttca ctcccagtgg agttaatccg
      601 aacatattta aaaaccaaat aaagaaaaag atggtcaata aatagctcat gtatttttta
      661 taattcttgt gcccgatgtt tggaatagca atgtcgtctc tgatataaag aatgattggc
      721 tcaaaaaatc gtccggctcc tgtagcaatt ccaccgtttt tagcatatga ttttgctagg
      781 cttgaaaata accataacat taatactcca actaaaaaca tcataaaaac gtttttagta
      841 attgaaaaat ctaatggttt aggattggtt gcgtggtgat ctgcatcata agaaatagtt
      901 ccctgagcat ctgttttata aattttacca tggtataatt tataaaagtt tccgttactt
      961 tctgctaccg attcaccatg gtgaaatttt gatgacatga aaaactgcac accattatca
     1021 actaaaataa ccggtaatgg aaaaccaacg tgatgttcaa cttttgaatc atcttcccaa
     1081 gaaaacaaat gaaaatcata cgaatctagt aagtgatgat taataaattc tttgatttcc
     1141 gtttttaaat cctttggttc ttctgaatgt tgagcttcaa cttctgtatg aacagcttct
     1201 gtatgaacta ctgtagaatc tgaactattt gcaaaagtaa gtagcggcaa aaccgaaact
     1261 aacagtgcaa ctataaaatg aagtggtttt tttgaaatca ccatattgtt ttttaactta
     1321 aatagttggg ttcctaaaat ttggtgcaaa ggtacatttt ttctttatta tgcaagtgta
     1381 ttgaaaaaaa aaatttgatt aaaacgcaac ttaaaagtgt gtttactgat ttttgttcaa
     1441 taatcgtatg gtgattagtg tttcaattgc taaaaataga atgaaaacgg caaaaaagtt
     1501 aactttctca atggtgtttt cactagaaga atttaaaata ggacgaagta taaaataaga
     1561 aactgccatt tttattgatg tacaaaccag gaatgtcatt ccggtagaat caaaattctt
     1621 ttcagaaact ttgataatca caaaaagaat gatttttgaa aagacaatat atataaaata
     1681 taaaaatgga atggagtaat aaaaatcttg agcggcattc ttcaacgttg attcttgaaa
     1741 aataataatg tgaatgataa aaattggaac tacaacaagt aggaatttat aaatgtccca
     1801 caattttttt atgcttatca ttcttctttg cttagttggt tcacttgacg atagacatta
     1861 tacaaagcta a
//
LOCUS       DAOTCW010000263         1856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_56107_length_1856_cov_46.246530, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000263 DAOTCW010000000
VERSION     DAOTCW010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56107_length_1856_cov_46.246530"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..>1856
                     /locus_tag="P5335_12120"
     CDS             322..>1856
                     /locus_tag="P5335_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016419260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRZ75672.1"
                     /translation="MLTVSNLSVQFGKRILFDEVNVTFTQGNCYGIIGANGAGKSTFM
                     KIISGEFDPTSGRVILEPGKRMSVLNQNHNMFDEYTVLETVMMGNKVLYDVKREMDEL
                     YLNYTDENSDRIGELQVQFEEMNGWNADSAAATLLSNLGITEDLHYSLMADMDGKLKV
                     RVLLAQALFGNPDVLIMDEPTNDLDFETIGWLENFLANYENTVLVVSHDRHFLDAVCT
                     HVSDIDFGKITHYSGNYTFWYESSQLAAKQRAQQNKKAEEKKAELEEFIRRFSANVAK
                     SKQATSRKKMIEKLNLDEIKPSSRRYPAIIFDQEREAGDQILNVVGLGASVEGEVLFK
                     NVDLNMAKGDKVVVFSKDSRATSAFYEILNGNQKPDAGTFDWGITTIQSYLPGDNHSF
                     FENDLTLVDWLRQWAKTEEERDEVYVRGFLGKMIFSGEEALKTGRVLSGGEKVRCMIS
                     RMMMLRANVLMLDEPTNHLDLESIQAFNNSLKNFKGSVLFTTHDHEFAQTVANRVLEI
                     TPNG"
BASE COUNT          595 a          283 c          397 g          581 t
ORIGIN      
        1 accacgtcat cctttaaaaa gaggacaaag ttgctttttg gattgatgac ttctcctcca
       61 aaataggctg taaaattatc atctttgaat tctttttgac acccgataaa agcataaagt
      121 gttgctacaa gacagataaa aggtattttt tttgaatagg caatcatttg gtggtattaa
      181 tttgaaacaa atatattttt tctactgttt acggcttgtt aatagacagt taaaataaac
      241 ctatatttat tctaaatagg tctggccaaa aagtgcttta ttttttctac ttttgcaacg
      301 ttttttaaaa tacacaatca catgttgaca gtttcaaatt tatcagttca attcggcaaa
      361 agaattttgt ttgatgaagt aaatgtaacc tttacccaag gcaattgcta tggaataatt
      421 ggggcaaacg gtgccgggaa atctacgttt atgaaaatta tttcaggaga atttgaccct
      481 acttcgggaa gagttatttt ggaacccgga aaaaggatgt ctgtattaaa tcagaatcat
      541 aacatgtttg atgagtatac ggttttagaa actgtaatga tgggaaataa agtgttgtat
      601 gatgtgaaac gggaaatgga tgaattgtac ctcaattata ctgacgagaa ttccgataga
      661 attggtgaat tgcaagttca atttgaagag atgaatggtt ggaacgcgga ttctgcagca
      721 gctacattac tgtctaactt aggcataact gaagatttgc attattcgtt aatggctgat
      781 atggatggaa aattaaaagt gagagtcctt ttggctcaag cactttttgg aaatcccgac
      841 gtgttgatta tggatgagcc cacaaatgac cttgattttg aaactatcgg ttggttagaa
      901 aatttcttag ccaattatga aaacaccgtt ttagtggtat cgcatgaccg tcacttttta
      961 gatgcggttt gtacacacgt atcggatatt gattttggaa aaataacgca ttattcgggg
     1021 aattatactt tttggtacga aagtagccag ttagcggcca aacaaagagc acaacaaaac
     1081 aaaaaggcag aagagaaaaa agcagagtta gaagaattta tccgtcgttt tagtgcgaac
     1141 gtagccaaat caaaacaggc aacttcccgt aaaaaaatga ttgaaaaatt aaatttagat
     1201 gagattaagc cgtcaagtcg tcgctatcct gccattatat ttgaccaaga gcgtgaagcg
     1261 ggtgaccaaa tattaaatgt tgtgggttta ggtgcttctg ttgaagggga agttttattt
     1321 aaaaatgttg acttgaacat ggctaaaggt gataaagtag ttgttttttc caaagattca
     1381 cgggcaacat ctgcttttta cgaaatatta aacggtaatc aaaagcctga tgcaggaacc
     1441 tttgattggg gtatcacaac gattcagtct taccttccgg gagataatca ttcctttttt
     1501 gagaatgatt taacccttgt ggattggttg cgtcaatggg caaaaacaga agaagaacgc
     1561 gatgaggttt atgtgcgtgg ctttttagga aaaatgattt tctccggaga agaagcattg
     1621 aaaacgggtc gtgttttgtc cggtggagaa aaagtgcgtt gtatgatttc cagaatgatg
     1681 atgcttcgtg ctaatgtgtt gatgttggat gaacctacca atcatttaga tttggaatcg
     1741 attcaggcct ttaacaactc gttgaaaaac ttcaaagggt cggttttatt caccacccat
     1801 gaccacgaat ttgctcaaac ggttgctaat cgtgtcttgg aaatcacccc taatgg
//
LOCUS       DAOTCW010000264         1829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_57425_length_1829_cov_33.594701, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000264 DAOTCW010000000
VERSION     DAOTCW010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57425_length_1829_cov_33.594701"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="P5335_12125"
     CDS             <1..926
                     /locus_tag="P5335_12125"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRZ75673.1"
                     /translation="FVRKSKEETYAQIETDLTDAIERLPIKGQYSPNDLGRATKGAAQ
                     ALLAKAYLYQEKWQLSYDMAGEVITSGQYGLLANYAEVWREVGENKVESIYEVQATLN
                     KGLVGYTDVQGARGTPDLGWGFNTPSLSLVNSYQEGDVRKNATVLFVQSTPFTLWDGF
                     EGSPTWNNPRYNYKAYQSSIAEPWNGNKGETAKNLRILKYSDILLIRAEAAYQNSNFG
                     EALARTNEIRVRAGLPELTSVTLADILNERRWEMAMEHDRWFDLVRTGQAPAAMAANG
                     KTFVVGTHELFPIPNNQIIASNGRLTQNPGY"
     gene            968..>1829
                     /locus_tag="P5335_12130"
     CDS             968..>1829
                     /locus_tag="P5335_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75674.1"
                     /translation="MKTNNFLLKSIALVVLGTAIVNCSDDDPAPLPPIGGYNNADEVA
                     AADLLAYWPLNGNGTESKSSTNPSSTVATTYVTGIKGQAASFNEGYMAYPAIAALSST
                     SGSASISCWAKISNTKLVPDGVSNISPIFSLTRTGETFGNLNLLGETHGLTTSDSIQM
                     KGVFRIKQEDGSEFGGDAVNMLKMEQWMIDGNANGENHQAFANKIGGQWAHIVYVFDG
                     AAGANKLYVNGVKISNPQWEVRNGGAPKMLNHYTPTRPVIGALETVVTGANGDAWNKA
                     LKGGVDELRVY"
BASE COUNT          626 a          296 c          377 g          530 t
ORIGIN      
        1 tttttgttcg aaaatcaaaa gaagaaacgt atgctcaaat agaaactgat ttaacagacg
       61 ctattgaacg attgcctata aaaggccaat attctcccaa tgatttaggt agagctacta
      121 aaggagcggc acaagcttta ttggcaaaag cctatttata tcaagaaaaa tggcaattgt
      181 cttatgatat ggcaggtgaa gtcatcactt ccggtcaata tggattgtta gctaattatg
      241 cagaagtttg gcgagaagtg ggcgaaaaca aagttgaatc tatttatgaa gtgcaagcca
      301 ctttaaacaa aggacttgtt ggttataccg atgttcaagg agccagagga acacctgatt
      361 taggttgggg tttcaataca ccatcattat cgcttgttaa ttcctatcaa gaaggcgatg
      421 taagaaaaaa tgcaacagtt ttatttgttc aatcaacccc ttttacatta tgggatggtt
      481 ttgaaggttc tcctacttgg aataacccac gttacaacta taaagcttat caaagcagta
      541 ttgcagaacc ttggaatggt aacaaaggag aaacagccaa aaaccttcgt attttgaaat
      601 acagcgacat tctcttaatt agagcagaag cagcctatca aaattcaaat tttggtgaag
      661 cattggccag aacaaatgaa atcagagtta gagcaggatt acctgaatta acctcagtaa
      721 ctttagctga tattttaaac gaacgccgat gggaaatggc gatggaacat gatcgttggt
      781 tcgatttagt aagaaccggt caagctcctg cagctatggc agcaaatggt aaaacatttg
      841 ttgtgggaac acatgaatta ttcccgattc ccaataatca gattattgcc agtaacggaa
      901 gattaacaca gaatccagga tattaagaaa aacaaattaa cattaattat ttaaattttt
      961 tacacttatg aaaacaaaca attttttatt gaaatctatt gcgttagtag ttttaggaac
     1021 agcaatagta aactgtagtg atgacgatcc ggctccacta cctccaatcg gtggttataa
     1081 caatgctgac gaagttgctg cggctgactt attagcttat tggccattaa acggaaacgg
     1141 tactgaaagt aaatcaagta caaatccttc ttcaactgtt gcaacaacgt atgtaacagg
     1201 tataaaagga caagcagcaa gttttaacga aggttacatg gcttatccgg ctatcgctgc
     1261 tttaagttca acttcaggta gtgcttctat cagttgttgg gcaaaaattt ctaatactaa
     1321 attagtacct gacggagtta gtaacatttc tccaattttc tctttaacaa gaacgggtga
     1381 aacctttgga aatttaaatt tattaggaga aacacatggt ttaacaacta gtgattctat
     1441 tcaaatgaaa ggtgttttca gaattaaaca agaggatggt tctgaatttg gtggtgatgc
     1501 cgttaacatg cttaaaatgg agcaatggat gattgatggt aatgccaatg gtgaaaatca
     1561 ccaagcattt gcaaataaaa ttggtggaca atgggcacat atcgtttatg tttttgatgg
     1621 tgctgcagga gctaataaac tttatgtgaa cggtgtgaaa atttctaacc ctcaatggga
     1681 agttagaaat ggtggagcac caaaaatgtt aaatcattac actcctacaa gaccggttat
     1741 cggagcttta gaaactgttg taacaggagc taatggtgac gcttggaaca aagctttaaa
     1801 aggtggagta gatgaactta gagtttata
//
LOCUS       DAOTCW010000265         1799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_58878_length_1799_cov_36.894495, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000265 DAOTCW010000000
VERSION     DAOTCW010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58878_length_1799_cov_36.894495"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..773)
                     /locus_tag="P5335_12135"
     CDS             complement(177..773)
                     /locus_tag="P5335_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRZ75675.1"
                     /translation="MQIDLNLLFTWGAIAKKYKKNDIIFYEDDLALFYYQIIEGSVRM
                     YNSNDEGKEFTQGIFCNGNSFGEPPLFINEVYPTTAVAAKDSVIIKLSKEKLMKILED
                     YPSVKLSFLELMAKRIYNKTISTKQIINQKPEHRIKSFLENYKKQNQLCNAEKSPIPY
                     TRQEIADFTGLRVETVIRTLSEMNKANKVIIDKHKLYF"
     gene            894..1229
                     /locus_tag="P5335_12140"
     CDS             894..1229
                     /locus_tag="P5335_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75676.1"
                     /translation="MRYFVILLFLYFSALTILPSVRALKLHFTENCQSSCQSENPENE
                     PSSGCQKEKCLLNFNFNTSVFLVFASTFELQKELFFNLEKANIHYHKNFISNFNVTIW
                     QPPELFLIV"
     gene            1287..1484
                     /locus_tag="P5335_12145"
     CDS             1287..1484
                     /locus_tag="P5335_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75677.1"
                     /translation="MKKIVSIVSVIAFLVSINVSAQQEPKQNKNKTKTEKSCSPDEKK
                     SCGTEKKGGCCSSSKTQEKKS"
     gene            1561..>1799
                     /locus_tag="P5335_12150"
     CDS             1561..>1799
                     /locus_tag="P5335_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75678.1"
                     /translation="MKTIFTIVFLFIFCFAFPQSTFDYQVQLNPITIPNFPGIHSYAF
                     GQHNGKWVIIGGRLDGIHARQPFNAFPVNSNNTNI"
BASE COUNT          646 a          251 c          236 g          666 t
ORIGIN      
        1 caattgcgtg acccaaccgg caaaagcaaa atgtgaatga gcatgttgga tatttttttg
       61 agctaaatag ggtaattcaa aagcaatttt ataccgcatt aaaacgccca aaaaagcaac
      121 aataaataaa ctgaaaagtg atatttttat ccaattttta atcgataatt ttttcattaa
      181 aaatataatt tatgcttgtc aataattact ttattcgctt tattcatttc agaaagtgta
      241 cgaattacag tttctactcg taaaccggta aaatcagcaa tttcttgtct ggtatatgga
      301 ataggagatt tttctgcgtt gcagagttgg ttttgttttt tataattttc taaaaatgat
      361 ttaatgcggt gttccggttt ttgattaata atttgtttgg ttgatatggt tttgttataa
      421 atacgtttgg ccattaactc caaaaaagaa agttttacag atggatagtc ttccaatatt
      481 ttcatcaatt tctctttaga tagtttgatt ataacactgt cttttgcagc aaccgctgtt
      541 gttggataga cttcattaat gaacaaagga ggttcaccaa aactatttcc attacaaaat
      601 ataccttgag taaactcttt tccttcatca ttcgaattat acatacgaac ggaaccttca
      661 ataatttgat aatagaataa agccaagtca tcttcataaa atataatatc atttttttta
      721 tattttttag ctatagctcc ccaagtgaat agtagattta aatctatttg catggtaatt
      781 tttaattaaa gatacaaata actttaattt aaaaatgaat aaaaatgata attatcatat
      841 gactgtgtaa caatagttac ataaaaataa aaaaatagca ctaaatttgt aatatgagat
      901 attttgtaat cttacttttt ttgtattttt ctgctttaac catcttacca tccgtaagag
      961 ctttgaagtt gcattttact gaaaattgtc aaagttcttg tcaaagtgaa aatcctgaaa
     1021 acgaaccttc tagtggatgt caaaaagaaa aatgtctttt aaattttaat tttaatacct
     1081 ccgttttttt agtatttgct tcaacttttg aattacaaaa agaattattt tttaatttag
     1141 aaaaagcaaa tatacattat cacaaaaact ttatttccaa tttcaatgta actatttggc
     1201 aaccgccaga actatttttg attgtttaaa tgttaatcgt aatcttatgg attacaaatt
     1261 cttaaattaa tttaaatttt aaaaaaatga aaaaaatagt ttcaatagta tcagtaattg
     1321 cttttttagt atcaattaat gtttcagctc aacaagagcc aaaacaaaat aagaacaaaa
     1381 caaaaaccga aaaatcatgt tctcctgacg aaaaaaaatc atgtggaact gagaaaaaag
     1441 gtgggtgttg ttcttcgagt aaaactcagg aaaaaaaatc ataaattctt aaatttatag
     1501 tttatacaat gcctattcaa taggcattgt attgctattt catattttaa aatttcactt
     1561 atgaaaacaa tatttacaat tgtatttctg tttatctttt gctttgcatt tccgcaatca
     1621 acttttgatt atcaggtaca attaaatcca atcacaattc caaattttcc cgggattcat
     1681 tcttatgcat ttggtcaaca taatggtaaa tgggttatca ttggcggaag attagatggt
     1741 attcatgctc gacaaccatt taatgctttt cctgttaata gtaataatac caacattta
//
LOCUS       DAOTCW010000266         1796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_59016_length_1796_cov_39.399196, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000266 DAOTCW010000000
VERSION     DAOTCW010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59016_length_1796_cov_39.399196"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..692)
                     /locus_tag="P5335_12155"
     CDS             complement(87..692)
                     /locus_tag="P5335_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75679.1"
                     /translation="MKKILPIFSYLFHPLFISVYASLLYFILFRDFYVLQEMYLFMIQ
                     IVIITVLIPISLFYLLLSLGKIDSIMIEQVAQRKIPILLNCILLFVLTQKSITLDRIP
                     ELYYFFLGGLFSSILVFILLLARKKASLHMVGIVALTFFVIGLSQCVEIPMLYTISIL
                     ILLIGFVASSRLVMKAHTFTELILGFICGALPQIIFWPLWL"
     gene            complement(694..>1796)
                     /gene="rpoN"
                     /locus_tag="P5335_12160"
     CDS             complement(694..>1796)
                     /gene="rpoN"
                     /locus_tag="P5335_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRZ75680.1"
                     /translation="LINQLNTFILSDEDRDIAEFLVGSIDDMGYIRRTVADIVDDLAF
                     TQGIYTDEQNVQRILQIVHELEPSGVGARDLQECLLLQLKHKTPTEYVDLAIDILENQ
                     FDQFTKKHYDKLLHKYGVSQEQLKKAIEEIEKLNPKPGGAFTGNNKMVEHVVPDFTIR
                     IVEGELELLLNGRNAPELHVSKDYQDMLRTYKDSRDKSNAQKDAVQFIKQKLDSAKWF
                     IDAIKQRQETLFVTMNAIMHYQQEFFLEGDETKLRPMILKDIADMIGLDISTVSRVAN
                     SKYVETPYGTKLIKEFFSESMKNDQGEDVSTLEIKKILQNVIEEEDKKKPLPDDQLAD
                     ILKERGYPIARRTVAKYREQLDIPVARMRKKM"
BASE COUNT          590 a          294 c          294 g          618 t
ORIGIN      
        1 acacgctttt tgattcaact gtagctaata ttggaggaaa ccctttagat gttagtgtct
       61 tacatttagg acttcaattt tatattttat agccataagg gccaaaatat aatttgaggt
      121 agtgctccac aaataaaacc caaaattaat tctgtaaatg tatgggcttt catcactaaa
      181 cgggatgaag ctacaaaacc aatcagtagt attaagatag aaattgtgta taacattggt
      241 atttctacac attggcttag gcctattaca aaaaaagtta atgcgacaat tccaaccatg
      301 tgtaaactag cttttttgcg tgctagtagt aaaataaata ctaatataga actaaacagt
      361 ccacctaaaa aaaaataata taattccggt attctgtcga gggttatgct tttttgggta
      421 agaacaaaca gtaatatgca gtttaaaagt attggaattt tacgttgtgc tacttgttca
      481 atcattatag aatcaatttt tcccaaagaa agtaaaagat agaaaagtga aatgggaatt
      541 aaaactgtaa taatgacaat ttgaatcatg aataaataca tttcttgcaa aacataaaaa
      601 tcacgaaaca aaataaagta cagcaaagaa gcataaaccg atataaaaag cggatggaag
      661 agataagaaa atataggaag tatttttttc aaattacatt ttcttacgca tcctagcgac
      721 cggaatatct aattgctctc tgtattttgc aacggttcgt ctggctatgg gatagcctct
      781 ttcctttaaa atatcggcta attgatcatc cggaagcggt ttctttttgt cttcttcttc
      841 aattacattt tgaagaattt ttttaatttc cagagtggat acatcttctc cttggtcatt
      901 tttcattgat tctgaaaaga attctttgat taatttagtt ccgtaaggtg tttccacata
      961 tttgctgttt gccacgcgag aaacggttga gatgtctaat ccaatcatat cggcaatgtc
     1021 tttaagaatc attgggcgaa gtttggtttc atcaccttct aaaaaaaact cttgttgata
     1081 atgcatgatg gcattcattg ttacaaataa ggtttcttga cgttgcttaa ttgcatcaat
     1141 aaaccatttg gctgaatcta atttttgttt gataaattga acggcatctt tttgagcatt
     1201 ggatttgtca cgtgaatctt tataagtgcg taacatatcc tgataatctt ttgaaacatg
     1261 aagttccgga gcattccttc cattaagcaa cagttcgagt tctccctcaa caattcgaat
     1321 ggtaaaatca ggaacaacgt gttctaccat tttattattt ccggtaaatg ctccacccgg
     1381 ttttggattc agtttttcaa tttcttcaat cgcttttttt aattgctctt gtgaaacacc
     1441 atatttatgt aaaagtttat cgtaatgctt tttagtaaac tgatcaaatt gattttctaa
     1501 aatatcaatt gctaaatcaa cgtattctgt gggtgttttg tgctttagtt gcaataataa
     1561 acattcttgt aaatcacgag ctccaacacc ggaaggttcc aattcatgaa cgatttgtaa
     1621 aatgcgttga acattctgtt cgtcagtata aataccttgt gtaaatgcta aatcgtcgac
     1681 aatatcagca actgttcttc tgatataacc catgtcatca atgcttccaa ctaagaactc
     1741 agcaatatca cgatcttcat cagaaaggat gaaggtgttt aattgattga ttaaat
//
LOCUS       DAOTCW010000267         1796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_59017_length_1796_cov_34.128087, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000267 DAOTCW010000000
VERSION     DAOTCW010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59017_length_1796_cov_34.128087"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1644)
                     /locus_tag="P5335_12165"
     CDS             complement(154..1644)
                     /locus_tag="P5335_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75681.1"
                     /translation="MKSAYLLESYDAILRQIYAHSIISNKEIIKELSKHESESLRIYK
                     VVITNRQGTESLKISKPFHSFHPKVKPLELKTIFDLNDFEIYLPILKKHIEFTGETIY
                     FAIDPEYPNTFYVCEKCAITKLNPKERFFLYCHQSLKLENQNIKLAIKEKVFKLKSTL
                     KIEHYIHKNQLAMESQLNKLIKKINPETNNELYEYSLDFDTTDCLKTIYSNLEKLLVF
                     IEKEYNEYLNVNIKVPQRTVLMNEYAIKPKLDFVRDSLMAKEMNQELLKIIFEPIIIL
                     STINIQNQITYYQFNYAIEYIYELTLLLHENPNLVNDSVIEEWLLDLNLNSFKYFDYK
                     TNKLKAELNKCESDFEQLELLYKKLKKFNQHQSKIIKAFNHKLPDSKVQICNWIEEEI
                     EFLNRKLNLTGKNQLAIKPMEVKNKILIGLSVAQISYFVSILMKTGIIKHANQRDVFR
                     MIAENFKTNGTDNISIDSLSSKFYNVETSTKNTIKEKIIELLNLTK"
BASE COUNT          520 a          266 c          304 g          706 t
ORIGIN      
        1 atgagagagc aacataattc ccaagttagt ccagcattgg ccacctttat gcgagtcaat
       61 gctatagctt ccttaaaaga gctactcctg atacctgatg aagttctcgt agaaatgcca
      121 ggttttggat ggcatttact gaaagaaatt ttactacttc gtaagattca ataattcgat
      181 gattttttct tttatggtgt ttttggttga tgtttccacg ttatagaatt ttgagctcaa
      241 actatctata gaaatgttat ccgtaccatt tgttttaaag ttctctgcaa tcattctgaa
      301 gacatctctt tggttcgcat gtttgatgat acccgttttc attaagatac ttacaaagta
      361 gcttatttgg gccaccgata aaccaatgag aattttgttt ttcacttcca ttggttttat
      421 cgctaattgg ttcttaccag taaggttaag cttgcggttt aggaattcaa tttcttcttc
      481 aatccaattg catatttgga ctttactatc agggagttta tggttgaatg cttttatgat
      541 tttactttga tgttggttga attttttgag tttcttgtat aaaagttcca attgttcaaa
      601 atccgattca catttgttca attctgcttt taatttattt gtcttataat caaaatactt
      661 gaatgaattc aagttgaggt caagtaacca ttcttcaatt acggaatcat tcaccaggtt
      721 tgggttttca tgtaacaata gcgtcaattc ataaatgtat tctattgcat aattgaattg
      781 gtaataggta atctgatttt ggatgttgat ggttgagagt ataattattg gttcaaatat
      841 aattttgagc agttcctggt tcatttcctt tgccattaat gagtctctca caaaatctaa
      901 ttttggtttt atggcatact cattcatcag cactgttcgc tgtggcacct tgatgttgac
      961 attcaaatac tcattgtatt ctttttcaat aaatactaac agcttttcca aattgctata
     1021 aatggttttc aggcagtctg tagtatcgaa gtcaagcgaa tactcatata gttcattgtt
     1081 ggtttccgga ttaatctttt tgatcagttt gttcaactgg ctttccatgg ccaattggtt
     1141 tttatgaatg tagtgctcaa tttttaaggt tgatttcagc ttgaacactt tttctttgat
     1201 ggctaatttg atattttggt tttccaactt tagagattga tggcaataga gaaagaaacg
     1261 ttcttttggg tttagctttg taattgcaca tttttcacac acataaaatg tgtttggata
     1321 ttctggatca attgcaaagt aaattgtttc acctgtaaat tctatatgct tttttagaat
     1381 tggtaagtaa atttcaaaat catttaaatc aaaaattgtc tttaactcca aaggctttac
     1441 tttggggtgg aatgaatgaa atggtttact aattttgagg gattcagtcc cttgtcgatt
     1501 agtaatcaca accttataaa tccgtaacga ttcggattca tgtttagaaa gttctttaat
     1561 tatttcctta ttggaaataa ttgaatgtgc ataaatctga cgtaaaatag cgtcatacga
     1621 ttcaagtagg taagcagatt tcatagtaag caaaaaaaat cctagcgtta attaaacact
     1681 tttaaaaact agaagtaaat ttgtttttgg atttttagtc aattcacaac actatgggca
     1741 atagtgttgt taaatttgtc gcaatttaaa aatttattta caattgttta actttt
//
LOCUS       DAOTCW010000268         1788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_59451_length_1788_cov_38.394691, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000268 DAOTCW010000000
VERSION     DAOTCW010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59451_length_1788_cov_38.394691"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..762
                     /locus_tag="P5335_12170"
     CDS             322..762
                     /locus_tag="P5335_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HRZ75682.1"
                     /translation="MTDISNRLEDHKINIRIVLSALWTAVMFCYIYGDYFELYVPKKV
                     EGLLNGQNMLDSPMKLLVATIILTIPALMIFLSLLLNSQLTKWLNIGVGLFFTLFTLL
                     VGISAMTEWRAFYVFLSFLESILTAIVVVKAWNWRKVKASHGNN"
     gene            800..1060
                     /locus_tag="P5335_12175"
     CDS             800..1060
                     /locus_tag="P5335_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75683.1"
                     /translation="MSYKKELIMNMTEQIKKNLISRIKDSKDLNFLNALQTIFDSSEQ
                     ALFQLSSEQQAAIEKGRNEIKNEQFSKNEEVISEMKQWLKKK"
     gene            1042..1341
                     /locus_tag="P5335_12180"
     CDS             1042..1341
                     /locus_tag="P5335_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRZ75684.1"
                     /translation="MAEKEIVWSKLAKLQLKTVLEFYTERNGNSTYSLKLLNEVEDLL
                     STLSKAERIGRLTSNKFTRVISMKVYLLFYEINGNRIEIVSFWDNRQDIENRKIK"
     gene            1433..>1788
                     /locus_tag="P5335_12185"
     CDS             1433..>1788
                     /locus_tag="P5335_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HRZ75685.1"
                     /translation="MFTIEQIKAAHSKVKSGADFPAYIQDIKQLGVAYYETFVWNGNA
                     DYYSKEGEKVSTGARYEALSIAKQSNTEQFMTDLKAHQQGKTDYPTFCSDCAKSGVEK
                     WVIRMDKMTCTYFDLAG"
BASE COUNT          609 a          270 c          337 g          572 t
ORIGIN      
        1 gaaaactact atatttactt catgattaat tgaaatcgat tttaagaggt ttaacgctat
       61 aactaaaaaa cagaaacgat gaaaggagta aactttttct ttttaatgat tgcctttatt
      121 ttagggatta ccttgtttag gcaattcaac tttgaaacga tgtcatttaa ggatccggca
      181 cttggtgtta tttaccttgt tacttttgta ttctcgattt tcattttaat taaggacttt
      241 ataaaacgtc cgaaaaagtg aatcactgcc ttccccgtaa caaaagttga cttttgatgt
      301 cttatatatg ataaatgagt tatgactgat ataagtaacc gattagaaga ccataaaata
      361 aacatcagaa tagtactttc tgccttgtgg acagcggtta tgttttgtta tatttatggc
      421 gattattttg aattgtatgt tcctaaaaaa gtggaggggc ttttaaacgg gcagaatatg
      481 ttggacagtc cgatgaaatt attagtcgcc acaattattt tgaccattcc ggctttgatg
      541 atttttcttt ccttactttt aaattcacaa ttgacgaaat ggttgaatat tggggtggga
      601 ctatttttta ccctttttac cttgcttgtg gggatttcag ctatgacgga atggcgagcc
      661 ttttatgtgt ttctttcttt tcttgaaagt atattgacgg caatagttgt tgttaaggct
      721 tggaattgga gaaaagtgaa agcgagtcac ggaaataatt gattacaaaa acggcataat
      781 tgaaaattaa tctttagttt tgagttataa aaaagaattg attatgaata tgactgagca
      841 aataaaaaaa aatttaattt caagaatcaa ggattcaaaa gatttgaact tcctcaatgc
      901 tcttcaaact atttttgatt cttctgagca ggctttattt cagttatctt ctgagcaaca
      961 agcagcaata gaaaaaggga gaaatgaaat caaaaatgaa caattctcta aaaatgagga
     1021 agtaatatca gaaatgaagc aatggctgaa aaagaaatag tttggtctaa acttgctaaa
     1081 cttcaattaa aaaccgttct tgagttttat actgaaagaa acggaaattc aacttatagc
     1141 cttaaactcc taaacgaagt tgaagatttg ttgtcgacac tctcaaaagc tgaacgtatt
     1201 gggagactta cgtcaaataa atttactcgt gttatttcca tgaaagttta tcttctattt
     1261 tatgaaatta atgggaatcg aattgagata gtatcttttt gggacaatag acaagatata
     1321 gaaaacagaa aaattaaata attttgttct gctaacctac gaaagtgcga gcataggctc
     1381 gtaacaacaa caaccccatt cgaaaaaatg tttaacaaac aataaataca gcatgtttac
     1441 tattgaacaa attaaagcgg cacacagcaa ggtgaaatcc ggggctgatt ttccggctta
     1501 tattcaggac atcaaacaac tgggcgtagc ctattatgaa acattcgttt ggaacggaaa
     1561 cgctgattat tacagtaagg agggagaaaa agtgtctacg ggtgcccgct atgaagcgtt
     1621 gtctatcgca aaacaatcca ataccgagca gtttatgact gaccttaaag ctcatcagca
     1681 gggcaaaaca gattatccca ccttttgttc ggattgtgca aaatcaggtg ttgaaaaatg
     1741 ggttatccga atggataaga tgacctgcac ctattttgac ttggcagg
//
LOCUS       DAOTCW010000269         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_60940_length_1761_cov_24.498828, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000269 DAOTCW010000000
VERSION     DAOTCW010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60940_length_1761_cov_24.498828"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="P5335_12190"
     CDS             <1..194
                     /locus_tag="P5335_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRZ75686.1"
                     /translation="AMAKLYASEIALEVANEAVQIHGGNGYVAEYHVERMMRDSKITQ
                     IYEGTSEIQRIVISRGLVS"
     gene            278..955
                     /locus_tag="P5335_12195"
     CDS             278..955
                     /locus_tag="P5335_12195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRZ75687.1"
                     /translation="MKNIIITGTSRGIGFEMALQFANAGHQVLTISRKTHKELIEHSN
                     ITCLFIDLSSEKELHKVYDFLSQTWKKVDIIIHNAGSLLLKPFSQTTQEDFEKVYKVN
                     VFAVANLTRVCLPFLQKGSHVVTISSMGGIQGSMKFAGLAAYSSSKGAVITLSELLAE
                     EYREQGISFNVLALGAVQTEMLQEAFPGYEAPLSAKEMADYIVNFSLNGTKYFNGRVI
                     QVSSSTP"
     gene            970..1572
                     /locus_tag="P5335_12200"
     CDS             970..1572
                     /locus_tag="P5335_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HRZ75688.1"
                     /translation="MSEVLLKYLPEHAVEPVFELIKTNSVHLKIVNERQTRHGDYRKH
                     PSGKHQITVNANLNKYRFLITLVHEIAHLAAFEKHGRFIKPHGNEWKMTFQQLMVPFI
                     RPEIFPNHVLPFVANHFRNPTASSDTDARLAFVLKQFDERKPDIHFIHEVPSGSFFRI
                     KTGKIFQKKGLRVKRYECLEVKTGKLYLFNANAEVEIIPG"
BASE COUNT          607 a          278 c          328 g          548 t
ORIGIN      
        1 gtgcaatggc aaaattatat gcttctgaaa ttgctttaga agtggccaat gaagctgttc
       61 aaattcacgg tggaaacggg tacgtagcag aataccatgt agaacgtatg atgcgtgatt
      121 caaaaattac acaaatttat gaaggaacct ctgaaattca acgcattgta atttcaagag
      181 gattggtttc ttaaattaag taaatcagta tattttaaaa ccctttgaga agaatcttaa
      241 agggtttttt gtatttttag ccattataaa tattgaaatg aaaaacatta taatcaccgg
      301 aacttcgcgt ggaattggat ttgaaatggc tttacaattt gctaatgccg gacatcaagt
      361 tttgacaatt tccagaaaaa cacacaaaga attaattgaa cattcaaaca ttacttgtct
      421 tttcattgat ttatcaagcg aaaaggaatt gcataaagta tacgattttc tatcccaaac
      481 ttggaaaaaa gtggatataa ttattcacaa cgccggaagt ttgttgttaa aaccattttc
      541 acaaaccact caagaagatt ttgaaaaggt ttacaaagta aatgtgtttg ctgttgctaa
      601 tttgacacgc gtttgtttgc cttttttaca aaaaggcagt catgtagtga caataagctc
      661 gatgggtggt attcaaggga gcatgaagtt tgccggattg gccgcctatt catcaagtaa
      721 aggggcagtg ataacacttt cagaattatt agcagaagaa tatagagaac aaggtatttc
      781 ttttaatgtt ttggcacttg gagccgttca gacagaaatg ttgcaagaag cttttccagg
      841 ctatgaagca ccactttctg caaaagaaat ggccgattat attgtcaatt tttcgttaaa
      901 tggtacgaaa tattttaacg gaagagtgat tcaggtaagt tcatcaacac cataatttat
      961 aatttattaa tgagtgaagt tcttttaaaa tatcttccgg aacatgcggt tgaacctgtt
     1021 tttgagttaa taaaaactaa ttcggtgcat ttaaaaatcg tgaatgaacg acaaaccaga
     1081 catggcgatt atcgaaaaca tccttccggg aaacatcaaa ttacggtaaa tgcgaatttg
     1141 aataaatacc gatttttaat cacactcgtg cacgaaattg cccatttggc ggcttttgaa
     1201 aaacatgggc gattcattaa acctcatggt aacgaatgga aaatgacttt tcaacaatta
     1261 atggtaccgt ttattcgtcc ggagattttt cccaatcacg tattgccttt tgtagccaat
     1321 cattttagaa atccaactgc aagcagcgac accgatgctc gattggcatt tgtcttaaaa
     1381 caatttgacg agcgaaaacc cgatattcat tttatccatg aagttccatc cggaagtttt
     1441 tttagaataa aaacaggaaa aatttttcaa aaaaaaggat tacgagtcaa acgctatgaa
     1501 tgcttagaag taaaaaccgg aaagttgtat ttgtttaatg cgaatgcaga ggtggagata
     1561 attccgggtt gattttagtt tctaatgatt tgtgttttgc tttttacatt acttcactta
     1621 aaataattta gcaatgtact aactcagcaa ctttactata tttgtatcag taaaaaaact
     1681 aagcggctaa gtaactcagc aactacaaaa atgaacaaaa attattacgc aatagtaatg
     1741 gccggtggag taggttcacg t
//
LOCUS       DAOTCW010000270         1739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_62107_length_1739_cov_40.755938, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000270 DAOTCW010000000
VERSION     DAOTCW010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62107_length_1739_cov_40.755938"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..710)
                     /locus_tag="P5335_12205"
     CDS             complement(267..710)
                     /locus_tag="P5335_12205"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRZ75689.1"
                     /translation="MENPFTDEYFMKKALQEAEIAFEKGEVPVGALIVVDNKIIARTH
                     NLTELLNDVTAHAEMQAITSAANFIGGKYLKNCTLYVTLEPCQMCAGALYWSQISKIV
                     FGARDEQRGFITLGTQLHPKTQVISGVLSQEASDLMVRFFASKRK"
     gene            749..>1739
                     /locus_tag="P5335_12210"
     CDS             749..>1739
                     /locus_tag="P5335_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HRZ75690.1"
                     /translation="MVENLLSYIDSPNDLRKLTVNQLPQLAQELRDFIIDVVSVKEGH
                     LGASLGVVELTIALHYVFQTPNDLLVWDVGHQAYGHKILTGRKAVFHTNRQLGGISGF
                     PKRSESEFDTFGVGHSSTSISAALGMAIASQLKGENKHHIAVIGDASIASGMAFEGLN
                     HAGVTDANLLVILNDNAIGIDPSVGALKNYLTAVKKGKNPKQNNMIKSLNFDYSGPID
                     GHDIYAIIAELERLKKIKGPKFLHVITTKGKGLQKAEEDQVKYHAPGKFDKLTGEIHL
                     KEESHLPPKFQDVFGLTLVELAQQNKKIIGITPAMPTGSSLKFMMDAFPERAFD"
BASE COUNT          579 a          297 c          322 g          541 t
ORIGIN      
        1 atgaaaccaa tcctgaactg aaattcagaa catagtcctt gaacaatgaa aaatcagaat
       61 taaattcttc agtagaaact tttttgccac aagaatgtag catcacaaca gcaacaaata
      121 gttgcgccaa acgtttgatt gacattgttc aaaatgatta aaagtggaac gaaaaaatga
      181 ggtttactta atttcgataa cacaaaaagt attccaaata ttgtgttaaa agtaattaat
      241 cttttaagaa gaaaaattac gttaatttat tttctttttg aggcaaaaaa acgcaccatc
      301 aaatcggatg cttcttgact caaaactcct gaaattactt gggtttttgg atgtaattga
      361 gtccctaaag ttataaaacc acgttgttca tctcgagctc caaaaacgat tttagaaatt
      421 tgactccagt acaatgctcc ggcacacatt tgacaaggtt ctaatgtcac atagagcgta
      481 caatttttta ggtattttcc cccaataaaa ttagcggcgg aagtaatggc ttgcatttca
      541 gcatgagcgg tgacatcgtt gagcaattcg gttaaattat gtgttcgtgc aatgatttta
      601 ttatcaacaa caatcaaagc cccaacggga acctcgcctt tttcaaaagc aatttctgct
      661 tcttgtaaag cttttttcat aaaatactcg tcggtgaaag gattttccat agaaacaaaa
      721 atacaatttc aattcgtaca tttgtgctat ggttgaaaat ttactttcct atattgattc
      781 tccgaatgat ttacgaaaat taaccgtaaa ccaattgcca caactcgctc aagaattacg
      841 agattttatc attgacgttg tttccgtaaa agaaggtcat ttaggagcca gtttgggtgt
      901 tgttgaattg acgattgctt tacattatgt ttttcaaacg ccaaacgatt tattagtttg
      961 ggatgttggc catcaagcgt atggtcataa aatattaacc ggaagaaaag ctgtttttca
     1021 taccaatcgt caattaggtg ggatttctgg atttccaaaa agaagtgaaa gtgaatttga
     1081 tacgtttgga gttggtcact cctctacttc tatttctgct gctttgggaa tggcaattgc
     1141 ctcccaactc aaaggtgaaa acaaacacca tattgctgtt ataggcgatg cttccattgc
     1201 gagcggaatg gcctttgaag gtttgaatca tgccggtgta actgatgcta atttgttagt
     1261 gattttaaat gacaatgcca ttggaataga ccctagtgtg ggtgcgttga aaaattatct
     1321 caccgctgtg aaaaaaggca aaaatccgaa acaaaacaac atgattaagt cgttaaattt
     1381 tgactattcc ggtccgattg acggtcatga tatttatgca attattgctg aattggaacg
     1441 attgaaaaaa ataaaaggtc caaagttttt gcacgtcata accacgaaag gtaagggact
     1501 tcaaaaagca gaagaagatc aagtaaaata ccacgctccc ggtaaatttg ataaactaac
     1561 tggtgaaatt catttgaaag aagaaagtca tttacctcct aaatttcagg atgtttttgg
     1621 tttgactttg gtagaattgg ctcaacaaaa taagaaaatt attggcatta ctcctgctat
     1681 gccaaccggt agctccttaa aatttatgat ggatgctttt ccggaaagag cttttgatg
//
LOCUS       DAOTCW010000271         1729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_62673_length_1729_cov_52.627838, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000271 DAOTCW010000000
VERSION     DAOTCW010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62673_length_1729_cov_52.627838"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="P5335_12215"
     CDS             <1..549
                     /locus_tag="P5335_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HRZ75691.1"
                     /translation="AGSALSANATAAMAFATTTVASASAMLTWIFFDRLHGRKVSALG
                     ACVGAVVGLVAITPAAGFISIPQSIFFGFIAAIVSNKMLYWKKLKSIDDTLDVFACHG
                     VGGIMGMILTAIFAEGENASLLHGGWSVFGHHMAALILVAGFTFFGSIILYKITNFII
                     PLRVSEESEIIGLDLSQHDEKF"
     gene            complement(582..1328)
                     /locus_tag="P5335_12220"
     CDS             complement(582..1328)
                     /locus_tag="P5335_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRZ75692.1"
                     /translation="MKIFKILFWLLWRVWFYILIGVIIVVLFPLLFVSILKESWYPFF
                     FRLAQVWARGILIGMGFFTKVVQKQKFKKGESYMLVANHTSMVDIMLMLVVAKNPFVF
                     VGKKELSKIPLFGFFYKRTCILVDRENSKSRLAVYERAQKRLNQGLSICIFPEGMVPE
                     DESIVLAEFKDGAFRLAIEHQIPIVPMSFLDNKKRLSYTIFSGSPGIMRVKMHEFIST
                     QGFTVDDKKAIRDKAWDVIHKELVASEKAK"
     gene            1466..>1729
                     /locus_tag="P5335_12225"
     CDS             1466..>1729
                     /locus_tag="P5335_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRZ75693.1"
                     /translation="MSKILTGIQATGTPHLGNLLGAILPAIEMANQPENESFLFIADL
                     HSITQIKDGSSLRENTYSVAASWLACGLDINKVVFYRQSDVPQT"
BASE COUNT          556 a          309 c          286 g          578 t
ORIGIN      
        1 gccggttctg ccttatcagc caatgctaca gcagcgatgg cttttgcaac cacaaccgtg
       61 gcttccgcct ctgctatgtt aacctggatt ttctttgatc gccttcacgg acgaaaagtt
      121 tctgctcttg gagcatgtgt aggagccgtt gttggattgg tggcgataac tcctgctgcg
      181 ggatttatta gtattcctca aagtatattc tttgggttta ttgccgcaat tgtgtcaaat
      241 aaaatgcttt attggaaaaa attaaaatct atcgatgata ctttagatgt ttttgcatgt
      301 catggcgttg gaggaataat gggaatgatt ttaaccgcca tttttgctga aggagaaaat
      361 gcaagtttgt tacacggagg ttggtcagtt tttggccatc acatggcggc cttaatttta
      421 gttgcaggat tcactttctt tggttcaata attttgtata aaattactaa ttttattatt
      481 ccgttaagag tttctgaaga gtctgaaatc attggtttag acttatcaca acatgatgaa
      541 aaattttaat ggtgaagtca gcaaagaaaa gtaagaaaaa tttattttgc tttttcagaa
      601 gctactaatt ctttatgaat tacatcccaa gctttatccc gtatagcttt tttgtcatca
      661 acggtaaacc cttgagtaga gataaattcg tgcattttaa ctcgcataat tccggggcta
      721 ccactaaaaa ttgtgtaaga aagtcttttt ttattgtcta aaaaagacat tggcacaata
      781 ggaatttgat gttcaatagc caaacgaaaa gctccgtctt taaattcagc taagacaatg
      841 gattcatctt cgggcaccat tccttcaggg aaaatacaaa tactgagtcc ttgattcaat
      901 cttttttgag ctctttcata tacggctaat ctgctttttg aattctctct gtctactaaa
      961 atacaagtgc gtttgtagaa aaaaccaaat aaaggaattt ttgataattc ttttttccca
     1021 acaaaaacaa atgggttttt agcaacaacc aacatcaaca taatatccac cattgaggta
     1081 tgattggcca ccagcatata gctttcccct tttttaaatt tttgcttttg aactactttt
     1141 gtaaaaaaac ccattccaat taaaattcct ctagcccata cttgagctaa tctaaaaaaa
     1201 aacggatacc atgattcttt caatatagaa acaaataata aaggaaaaag aacaactatg
     1261 attactccta taaggatata aaaccacact cgccaaagca accaaaatag aattttaaaa
     1321 attttcatga agccaaatgt aagcaaagca atgtaaatat taaatcatat aactaacttt
     1381 gttcttttca aaaaatattt aaccccttaa ttttgcaacc tttttagaaa actttgcgaa
     1441 ctttgcagat tatataccaa gcattatgtc aaaaatactc accggaatac aagccacagg
     1501 cactcctcat ttaggtaatt tattaggtgc tattttaccg gccatcgaga tggctaatca
     1561 gcctgaaaat gaatcttttt tattcattgc tgatttacat tctattacac aaataaaaga
     1621 tggttcatcg ttacgcgaaa atacgtatag tgttgcggca tcttggttag cttgcggttt
     1681 agatattaat aaagttgttt tttatcgtca aagtgatgtg ccgcaaaca
//
LOCUS       DAOTCW010000272         1694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_64726_length_1694_cov_39.079317, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000272 DAOTCW010000000
VERSION     DAOTCW010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64726_length_1694_cov_39.079317"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="P5335_12230"
     CDS             <1..844
                     /locus_tag="P5335_12230"
                     /inference="COORDINATES: protein motif:HMM:NF017822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HRZ75694.1"
                     /translation="KNSMFVTDENLLIYEGETFFEIYTSGMNLEFNLKLAEGYKSNSQ
                     IARRLTEDWVLHQSYCPSCGKLPLFEFENNRPVADFYCNDCSEEFELKSKEGKLTSKV
                     TDGAYSTMIERIQSNNNPNFFFLTYSKNWTVDNFLIVPKQFFTPDIIIKRKPLSLTAK
                     RAGWTGCLIDLSKVASAGKVFLVKDAQSVSPKKVQESFQKTLFIREKKTESKGWILDV
                     LQCVDSIKKDSFTLDEIYKFEEQLKIKYPNNNFIKDKIRQQLQVLRDQGIIEFNGRGN
                     YKKI"
     gene            845..1048
                     /locus_tag="P5335_12235"
     CDS             845..1048
                     /locus_tag="P5335_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75695.1"
                     /translation="MKRYIFISPEGTTEAPNTLYEVNNMQVIGIVENVSNEDEALKKL
                     LLENEWIFDAEFNVAEFICYEIA"
     gene            1144..1605
                     /gene="tnpA"
                     /locus_tag="P5335_12240"
     CDS             1144..1605
                     /gene="tnpA"
                     /locus_tag="P5335_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRZ75696.1"
                     /translation="MANTYTQLYIQFVFAVKYREAMLLSSFKTELHQYIGGIINHNKH
                     KLLAINGMPDHLHVLISMKPSQSMSELMQDIKGSSSKWINEKKFLNVKFEWQEGYGAF
                     SYSKSHVDAVIKYISNQEQHHKTKTFQEEYLDFLKAFEIDFDERYVFKEPI"
BASE COUNT          630 a          221 c          295 g          548 t
ORIGIN      
        1 taaaaacagt atgtttgtta ctgatgaaaa tttattaatt tatgaaggcg aaaccttttt
       61 tgaaatatat acttcaggaa tgaatttaga attcaactta aaattagctg agggatataa
      121 aagtaattct caaatagcca gaaggttaac tgaggattgg gttttgcatc aatcgtattg
      181 tccttcttgt ggaaaattac ctttatttga atttgaaaat aatcgacctg ttgccgattt
      241 ttattgtaac gattgtagtg aagaatttga attgaaaagt aaagaaggaa aattaacttc
      301 aaaagtaaca gacggagctt attctacaat gattgaaagg attcaatcaa ataacaatcc
      361 taattttttc tttttgactt attcaaaaaa ttggacagtt gataattttt tgatagtacc
      421 gaaacagttt tttacacctg atattattat taagagaaaa ccattatctc ttacggcaaa
      481 gcgtgccggt tggactggtt gtttgattga tttatcaaaa gtagcatcag caggaaaagt
      541 ttttttagtg aaagatgctc aatctgtttc tcctaaaaag gttcaagaat catttcaaaa
      601 gacacttttt atcagagaaa aaaagactga atcaaaaggt tggattttag atgtattgca
      661 gtgtgttgat tcaataaaaa aagattcctt tactttagat gaaatttata aatttgaaga
      721 gcagttaaaa attaaatatc caaataataa ttttattaaa gataagatta gacaacaatt
      781 acaagtttta agagaccaag gaattattga atttaatgga cgaggaaatt ataaaaaaat
      841 ataaatgaaa agatatatat ttatttcacc cgaaggaact acagaagcac caaacacttt
      901 gtatgaagta aataacatgc aagtaatagg tattgtagag aatgttagta atgaagatga
      961 agctttaaaa aaacttttac ttgaaaacga atggatattt gatgctgagt ttaatgtagc
     1021 tgagtttatt tgttatgaaa ttgcgtaaaa aatcccgtag ggatgtaatg tatgtaacaa
     1081 cgggtttcaa cccgttgaaa aaatagcgaa aaccaatcaa gaatcccgtg gggatgatat
     1141 actatggcaa acacctatac acaactctat attcaatttg tatttgcagt aaaatatcga
     1201 gaagcaatgc tcctttcctc tttcaaaaca gaattgcatc aatacattgg aggcatcatt
     1261 aatcataata agcataaact cttagccatt aacggaatgc cagaccatct tcatgttttg
     1321 attagcatga aaccatcaca atctatgtct gaattaatgc aagacatcaa aggaagttca
     1381 tccaaatgga taaatgaaaa gaagttttta aacgttaaat ttgaatggca agagggatat
     1441 ggtgcttttt cttattcgaa atcgcatgtt gatgcggtta ttaaatatat atctaatcaa
     1501 gaacagcatc ataagacaaa aacgtttcaa gaggaatatt tggatttttt gaaggcattt
     1561 gagatagact ttgatgagcg ttatgttttt aaagaaccca tttaatatat catccctacg
     1621 ggattttgtt tgttgggggt tattattttc catgggttga aacccacggc tacaaatata
     1681 tcatccctac ggga
//
LOCUS       DAOTCW010000273         1661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_66848_length_1661_cov_42.929639, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000273 DAOTCW010000000
VERSION     DAOTCW010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66848_length_1661_cov_42.929639"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="P5335_12245"
     CDS             complement(<1..455)
                     /locus_tag="P5335_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRZ75697.1"
                     /translation="MEAIQTKSFKEQITEGIPSVLPAIKPYEIEINHAPKRKEILSAE
                     EKKLALRNALRYFDAEFHATLLPEFKDELEKYGRIYMYRFRPDYRMYARPISDYPGKC
                     EQAKAIMLMIQNNLDYAVAQHPHELITYGGNGAVFQNWAQYRLTMKYLS"
     gene            complement(458..640)
                     /locus_tag="P5335_12250"
     CDS             complement(458..640)
                     /locus_tag="P5335_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HRZ75698.1"
                     /translation="MNTPNSENKLIEGEDFYFTAEGYKCFTEKYHLKRGYCCKSGCRH
                     CPYGFDKKTGQLKPKK"
     gene            669..995
                     /locus_tag="P5335_12255"
     CDS             669..995
                     /locus_tag="P5335_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75699.1"
                     /translation="MIVIVFKYLTPKGFRGLTLFPFVFLKHHGDKHNAVLLNHERIHI
                     RQQIELLIIPFFIWYGLEFLFKWIKYKDRNLAYRSICFEREAYQNEKNLYFLKQRSFW
                     NFFNYF"
     gene            complement(992..>1661)
                     /locus_tag="P5335_12260"
     CDS             complement(992..>1661)
                     /locus_tag="P5335_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75700.1"
                     /translation="LERNRMWFDIVNSNGINDRTLVGYIEGATMDRDSFFDCNTAVLG
                     SMTVYSILNEERLSIQGRSLPFDENDIVPMGVYVPESGTYTFALAAVDGLFETQTIYL
                     KDKFLNITHNIKTNPYTFSSEQGSFDNRFEIVYLNETLSNPDFSLENEIRVVTNEFVN
                     VYSTVEPIESIIVYNVLGQKLKEYNNVNANSFKLTSLQKNNTTLLLKIKLQNELIKIE
                     KVIY"
BASE COUNT          517 a          266 c          262 g          616 t
ORIGIN      
        1 tcagaaagat atttcattgt caatcgatac tgagcccaat tttgaaaaac agcaccattt
       61 ccaccgtagg taatcaactc atgtggatgt tgagctactg cataatccaa gttgttttgt
      121 atcatcaaca tgattgcttt tgcttgttca cattttccag gatagtcaga aattgggcgt
      181 gcatacattc gataatcagg gcgaaagcga tacatgtaaa tacggccata tttttctaat
      241 tcatctttga attcaggtag tagagtagca tgaaattctg catcaaagta ccgaagtgca
      301 tttcgcaaag ccagtttttt ttcttcagca gaaagaattt cctttcgctt tggagcatga
      361 tttatttcaa tttcataagg ttttatagct ggtaatacgg atggaattcc ttctgtgatt
      421 tgctctttga atgacttggt ttgtattgct tccatgttta tttttttggt tttaactgtc
      481 cggttttttt atcaaagcca tagggacaat gtcgacagcc acttttacaa cagtagcctc
      541 tttttaaatg gtatttttct gtaaaacact tataaccttc ggccgtaaaa taaaaatctt
      601 cgccttcaat taatttattt tcagaatttg gtgtattcat agctacaaaa ttagcaactt
      661 tgtcacaaat gatagtgatt gtttttaaat atttaacgcc aaaaggtttt cgaggtctta
      721 cgttgtttcc atttgttttt ttaaagcacc atggagacaa gcacaatgca gttttgttga
      781 atcacgaaag aattcatatt cggcaacaaa tcgaattatt aattattcct ttttttattt
      841 ggtacggtct tgaatttcta tttaaatgga taaaatataa agatagaaac ttagcctatc
      901 gaagtatctg ctttgaaaga gaagcttatc aaaatgaaaa aaacctttac tttttaaagc
      961 aaaggtcttt ttggaatttc tttaattatt tttaataaat tactttttca attttaatta
     1021 attcattttg taatttgatt ttcaacagca atgtagtatt gtttttttga agcgacgtta
     1081 gtttgaatga attagcattc acattgttgt attcttttaa tttttgtcct aaaacgttat
     1141 aaacgattat tgattcaatt ggttcaacag ttgaataaac gtttacaaat tcattagtca
     1201 ctactctaat ctcgttttca agggaaaaat caggattgct aagtgtttcg ttcaaatata
     1261 caatttcaaa acgattgtca aatgaacctt gttcagatga aaaagtataa ggatttgttt
     1321 ttatattgtg cgttatgttt aaaaacttat cttttaaata gatggtttgg gtttcaaata
     1381 agccatctac cgcagctaaa gcgaaagtat atgtcccact ttcaggtaca taaacaccca
     1441 ttggaacgat atcattttca tcaaaaggaa ggcttcttcc ttgaatagaa agtctttctt
     1501 catttaagat agaataaaca gtcattgagc ctaatacagc ggtattacaa tcaaaaaagc
     1561 tgtctctgtc catggttgct ccttcaatat aacctactaa agtacggtca tttattccgt
     1621 ttgaattaac aatgtcaaac cacattctgt ttcgctctaa a
//
LOCUS       DAOTCW010000274         1652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_67438_length_1652_cov_31.964934, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000274 DAOTCW010000000
VERSION     DAOTCW010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67438_length_1652_cov_31.964934"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="P5335_12265"
     CDS             complement(<1..736)
                     /locus_tag="P5335_12265"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRZ75701.1"
                     /translation="MIKTIKKIGVLTSGGDSPGMNAAIRSVVRTCAYYKVECLGIYRG
                     YQGMIEGDFKDMGPRSVHNIVNKGGTILKSARSKEFMTAEGRKLAYENLVKENVDALV
                     VIGGDGSFTGALVFNKEYGIPVMGIPGTIDNDIFGTSHTLGFDTALNTVVEAIDKIRD
                     TASSHNRLFLVEVMGRDAGHIALNAGIGAGAEEILIPEENMGLERLLESLRRSKLSGK
                     SSSIVVIAEGDKIGKSVFELKDYIDQN"
     gene            complement(889..>1652)
                     /locus_tag="P5335_12270"
     CDS             complement(889..>1652)
                     /locus_tag="P5335_12270"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRZ75702.1"
                     /translation="INGTISNPDPDFNINLPTVSSTLKSEIQTVLDDKDTRQTQALYL
                     LATDSFLSEEGANVGQNALYNNMFQTMSGVFDDLFFDADSKISIGVGYVAPEKNPNGD
                     TNGNVSVSTSFNINERISVNGVIGVPVGDVNQAAVIGDLEVLYRVNDDGSLNFKVFNR
                     ENDINYIGEGVGYTQGIGITYQVDFDTFDELYNKVFRSAKYKLEQENKPKDEVPDSNY
                     TPEFINFTEPKKNLPEKPKSEKEKIEPPLENVPEE"
BASE COUNT          506 a          338 c          229 g          579 t
ORIGIN      
        1 ggttttgatc gatgtaatct tttagttcaa aaacgctttt accaatttta tcaccctcag
       61 caatgacgac aatacttgat gatttaccgg aaagcttact tcttcgtaac gattccaata
      121 aacgctctaa tcccatattt tcttccggaa ttagaatttc ttctgcacca gcaccaattc
      181 ctgcatttaa agcgatatgt cctgcatctc ttcccattac ctctactaaa aataagcgat
      241 tatgcgaact agctgtatct cttattttat caatcgcttc aacaactgta ttaagggctg
      301 tatcaaaacc taatgtgtgg ctagttccaa agatatcgtt gtcaatcgtc cccggaattc
      361 ccataaccgg tattccatat tctttattaa aaactaaagc tccggtaaaa ctaccatctc
      421 cgcctataac aactaatgca tcaacatttt ctttaactag attttcgtag gctaatttcc
      481 gaccttcagc agtcataaac tcttttgatc gggctgattt taaaattgta ccacctttgt
      541 ttacaatatt gtgaactgaa cgtggcccca tatctttaaa atcgccttca atcattcctt
      601 ggtaacctct gtaaattccg agacattcta ccttgtaata agcacaagtt ctaacgaccg
      661 aacgtattgc tgcattcatt ccgggagagt ctcccccaga tgtaagtaca cctatttttt
      721 ttattgtttt aatcatttta ttatgtttat aatgtaaaat taacaaagaa atgcgactta
      781 aaagcgtgtg tttttgagta tttgataatt tcttccaaaa tcaatcgttt tcgttgataa
      841 gtttctaatt tatcataaaa aaagataaaa tttggattta aaattcagtt attcttccgg
      901 aacattctca agaggtggtt ctattttctc cttttcggat tttggctttt caggtaaatt
      961 tttcttcggt tcagtgaaat ttataaattc aggagtataa tttgaatccg gaacttcatc
     1021 tttaggttta ttttcttgct ccaacttata tttagcactt ctgaaaactt tattatacaa
     1081 ttcatcaaag gtatcaaaat cgacttggta ggttatccca attccttgtg tataaccgac
     1141 accctcacct atataattta tatcattttc cctattaaaa actttaaaat taagagaacc
     1201 atcatcattg acacggtaaa gcacttcaag gtcgccaatc acagcagctt gatttacatc
     1261 acctaccgga acaccaatca caccatttac cgaaattcgt tcatttatgt taaatgaagt
     1321 agaaacactt acgtttccgt ttgtatcccc atttggattt ttttccgggg cgacatatcc
     1381 aactccaata gaaattttac tatctgcatc aaaaaacaaa tcatcaaaaa caccactcat
     1441 ggtttgaaac atgttattat ataaggcatt ttgacctaca tttgctccct cttcactcaa
     1501 aaaactatca gtagccaata aataaagagc ttgtgtttga cgtgtatctt tatcgtctaa
     1561 aacggtttgg atttcggatt ttaaggttga acttactgtg ggcaagttaa tattaaaatc
     1621 agggtcagga ttacttatag ttccattaat aa
//
LOCUS       DAOTCW010000275         1638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_68402_length_1638_cov_41.730891, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000275 DAOTCW010000000
VERSION     DAOTCW010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68402_length_1638_cov_41.730891"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="P5335_12275"
     CDS             complement(<1..1228)
                     /locus_tag="P5335_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRZ75703.1"
                     /translation="MMKSLYIFLFLLSVSCSWAQPNSQQKLEERKAQLLEEIRANERM
                     LKETKKKEKSVVAVIQQQNAKIQLREKLISTTEKQAKLLNDDIYTNQLKINKLNKELE
                     VLKEDYANMIVKSYKSRSERSRAMFLLSSQNFMQAYKRVQYMKQYAGYRKSQGDEIKV
                     KSTELAAYNEKLAGLKIEKIKVIQEQEKEKQVLVKEKKEQEILVNSIKKDQKKIIAEI
                     KKKQQEANEIDKKIQRFIRDAIAAENKKTAKKAGPKTVSTGTSATKIVLTKEGKIESD
                     NFKANKGKLPWPVDKGFVSQKFGNQPHQIQPKLIVHSNGVEITTESGSSARAVFAGTV
                     YSIQILSPINTLVMIQHGDFITVYQNLSSVSVKKGDKVSIKQTIGKIRTNNSTGKTAL
                     KFSVLQNDTFLNPQSWI"
     gene            complement(1241..>1638)
                     /locus_tag="P5335_12280"
     CDS             complement(1241..>1638)
                     /locus_tag="P5335_12280"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HRZ75704.1"
                     /translation="LDFYKVQNMLVGQAMDDLRKGKYSNAIEDRFYKLEDTIDKINKK
                     SFFFESSKFLLKKQEIEQVAKNRKLKVSYPHHKEYKEAILPLNILIEALQDNQKNTIS
                     LDYNTVSFNESLSFPYSVPDGYERIYIEN"
BASE COUNT          440 a          256 c          237 g          705 t
ORIGIN      
        1 aaatccaact ttgaggattt aagaacgtat cattttgtag tactgaaaat tttaatgctg
       61 tttttccggt agaattattt gttcggattt taccaattgt ttgtttaata cttactttat
      121 ctcctttttt aactgaaacc gaactaaggt tttgataaac ggttataaaa tctccgtgtt
      181 gaatcattac aagtgtatta atgggagata aaatttgaat agaataaact gttccggcaa
      241 aaacggctct agccgatgaa cctgattctg ttgtaatttc taccccattg ctgtgcacaa
      301 ttaatttagg ctgtatttga tgcggttgat taccaaactt ttgcgaaaca aatcctttat
      361 caaccggcca tggcaattta cctttattag ctttaaaatt atcagactca atttttcctt
      421 cttttgttaa aacaattttt gtagcagaag ttccggtgga aactgtttta gggcctgctt
      481 ttttggctgt ttttttattt tccgcagcaa tagcatctct aataaaacgc tggatctttt
      541 tatctatttc atttgcttcc tgctgtttct ttttaatttc agcaattatt ttcttttgat
      601 cttttttaat ggaattgact aaaatttctt gttccttttt ttcttttacc aacacctgtt
      661 tttctttttc ctgttcttga ataactttta ttttttcaat cttaagtccg gctaattttt
      721 cattgtaagc tgctaattca gttgatttta ctttaatttc atctccttgg ctttttcgat
      781 aacctgcata ttgcttcata tattgaactc ttttgtaagc ctgcataaag ttttgtgatg
      841 aaagtaaaaa catggctctg cttcgttctg aacggctttt ataagacttt acaatcatat
      901 tggcgtaatc ttcctttaaa acctctaact ctttgttgag tttgtttatt tttagttgat
      961 tcgtataaat atcatcattc agtagctttg cttgtttttc tgtagtgctt attaattttt
     1021 cacgcaattg aatttttgcg ttttgctgtt gaataacagc aacaactgat ttttctttct
     1081 ttttagtttc cttaagcatt ctttcattag ctctgatttc ttctaataat tgagcttttc
     1141 gttcttctag tttttgttgt gaattaggtt gtgcccacga acatgaaact gaaagaagaa
     1201 aaagaaatat gtataaagac ttcatcattt tttttattat ttaattttca atatatattc
     1261 tttcatatcc atccggaacg ctatagggga aagataatga ttcattaaaa gaaactgtgt
     1321 tataatctaa tgaaattgta ttcttttgat tgtcttgtaa agcttctatc agaatattta
     1381 atggtaaaat agcctctttg tattctttgt gatgaggata ggaaacttta agttttctat
     1441 ttttggccac ttgttcgatt tcttgtttct ttaataaaaa tttggatgat tcaaaaaaga
     1501 atgatttctt gtttatttta tcgattgtgt cttctaattt ataaaaacgg tcttctattg
     1561 cattgctgta ttttcctttt cgtaaatcgt ccattgcttg tccgacaagc atattctgaa
     1621 ctttataaaa atctaaat
//
LOCUS       DAOTCW010000276         1629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_69008_length_1629_cov_29.636595, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000276 DAOTCW010000000
VERSION     DAOTCW010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69008_length_1629_cov_29.636595"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1417)
                     /locus_tag="P5335_12285"
     CDS             complement(113..1417)
                     /locus_tag="P5335_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRZ75705.1"
                     /translation="MKNFKYIVIFLIGLPLFAVGQTVLTLEEAIAITLENNFDLRIAK
                     NEVKINEENVSLGNAGMLPRVNGIVTNNNTILNTKQTQSNGNEIEIDGAKNLNLNYGV
                     GLEWTIFDGFSMFARHNQLKEIQKLGEIELKLNVLAKVSEVYDTYYILVNQQHLIRSL
                     DSIIAVSEFRLKTAQTRFTIGKASKLEVLNAEVDYNADVSAKIRLKEQYAISKIALNQ
                     LLVRPSETDFTVTDVILIDETLKLTDLLALAQEQNPELQIQYINKKIQEYELKQVKAA
                     RYPSLSLSTGYNFIRSESPFGFITQTSGQNFNYGFTASINIFDGFNQNRNERTSKIQL
                     ENTQLQIDRQKQAVNAQISSFYQSYLSNLALLEMEQKNEKIARQNLEITLDKFKIGTI
                     SPVEFRTAQVNYANAVIRLTNAELLAKQTEISLKEIAGNLSF"
     gene            complement(1427..>1629)
                     /locus_tag="P5335_12290"
     CDS             complement(1427..>1629)
                     /locus_tag="P5335_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRZ75706.1"
                     /translation="GAMPIAVSLGAASQSRMGMGIVIVGGTIFSLILTLYVIPAIYLM
                     WSREKKHRTELDQADQFDRESV"
BASE COUNT          501 a          286 c          261 g          581 t
ORIGIN      
        1 acagcataaa aattcatttc cataagttaa aaagttattg gttagtatat caaatttaaa
       61 atataaatga ataacttgtt tcaaaatagg caataaaatg atgttcaaaa agttaaaagg
      121 ataagtttcc ggcaatttct tttaaactga tttcggtttg ttttgccaac aattctgcat
      181 tggttaaacg aatcaccgca ttggcataat tcacttgagc ggttctgaat tcaactggag
      241 atatagtccc aattttaaat ttgtctaaag tgatttctaa attttgacgg gcaatttttt
      301 cgttcttttg ttccatttcc aataaggcta aattagaaag atacgattga taaaaactag
      361 agatttgggc attaaccgct tgcttttgtc tatcaatctg caattgggta ttttccaatt
      421 gaattttaga cgttcgttca ttacggtttt gattgaatcc gtcaaatata ttgatagagg
      481 ctgtaaaacc ataattaaag ttttgcccgg aagtctgcgt tatgaatcca aaaggagatt
      541 ccgatcgaat gaaattatat cctgttgata aactcaaact tgggtatctt gctgctttca
      601 cttgctttag ttcgtattcc tgaatctttt tattgatgta ttgtatctgt aattccggat
      661 tttgttcttg ggccaatgcg agcaaatcgg ttaattttaa agtttcatca attaaaataa
      721 catcagttac cgtaaaatct gtttcagagg ggcgaactaa caattgattc aaagcgattt
      781 ttgaaatggc atattgctcc tttaacctga tttttgcgga aacatctgca ttgtaatcca
      841 cttcggcatt cagcacttct aattttgaag ctttaccaat ggtgaatcgt gtttgtgctg
      901 tttttaatcg gaattcggaa acagcgataa tactgtctaa acttctaatc aaatgttgtt
      961 gattgaccaa aatataatag gtgtcatata cttcactgac ttttgcaagc acgtttaatt
     1021 ttaattcaat ttcacccaat ttttgaattt ctttcaattg gttgtgacgt gcaaacatac
     1081 tgaaaccatc aaaaatggtc cactctaagc caacgccgta atttagattt aaattttttg
     1141 ctccgtcaat ttcaatttcg ttaccattcg attgtgtttg tttagtgttt aaaatggtat
     1201 tgttattagt aacaatccca ttcactcttg gcaacattcc ggcatttccc aaacttacat
     1261 tttcttcatt tattttaact tcgttttttg caatacgaag gtcaaaatta ttctctaatg
     1321 taatggcaat cgcttcttct aaggttagaa cagtttgtcc aacagcaaaa agaggaagtc
     1381 ctattagaaa tattactatg tatttaaaat ttttcatatt ttcaaattaa actgactcac
     1441 ggtcaaattg atcagcttgg tctaattcag tccggtgttt cttttcacgc gaccacatca
     1501 ggtaaatggc aggaatcaca taaagggtta aaattaacga gaaaatagtt ccccccacaa
     1561 ttacaattcc cattcccatt ctactctgag aagcagcccc taacgaaacg gcaattggca
     1621 tcgctccta
//
LOCUS       DAOTCW010000277         1622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_69500_length_1622_cov_31.119974, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000277 DAOTCW010000000
VERSION     DAOTCW010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69500_length_1622_cov_31.119974"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="P5335_12295"
     CDS             <1..644
                     /locus_tag="P5335_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRZ75707.1"
                     /translation="FKAIKVSNVSVTLACLSTGAFFTSFLEPILYRRKVIWYEVLFGL
                     IVVGGLYIIFNVEGDYYLGIILALTSAFLSALFSVINGKYAKMYDSTVISFYELTGGV
                     LCFSIYLLLSNSFSKDFFILQPSDWVYLLILSSICTAYAFIASVRVMKFISPYTVMLT
                     INLEPVYGIILALLIFKDKEKMSSEFYFGAILILITVILNGVIKYKLRNRNSS"
     gene            761..>1622
                     /locus_tag="P5335_12300"
     CDS             761..>1622
                     /locus_tag="P5335_12300"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRZ75708.1"
                     /translation="MEYLDFELPIKELEDQLDKCQIIGEESNVDVASTCKQIEKKLEE
                     TKKKIYKNLSAWQRVQLSRHPSRPYTMDHVRALCGNTFLELFGDRNFKDDKAMIGGLG
                     KIGGQSFMIVGQQKGFNTKTRQFRNFGMANPEGYRKALRLMKMAEKFGIPVVTLIDTP
                     GAYPGLEAEERGQGEAIARNILEMIRLKTPIITVIVGEGASGGALGIGVGDKVYMLEN
                     TWYSVISPESCSSILWRSWEYKEQAAEALKLTSYDMKKQKLIDEIVPEPLGGAHYDRE
                     TTFKTVEQYII"
BASE COUNT          537 a          218 c          300 g          567 t
ORIGIN      
        1 tttttaaagc cattaaagtc tctaatgttt ctgtcacttt agcgtgttta tcaacaggtg
       61 ctttttttac ctctttttta gaaccaattt tataccgacg aaaagtcatc tggtatgaag
      121 ttttatttgg actaattgta gttggcggat tatatatcat ttttaatgtt gaaggagatt
      181 attatttagg gataatactt gccttaacgt cagcattttt atcggctctt ttttctgtaa
      241 ttaatgggaa gtatgcaaag atgtatgatt caacagttat ttctttttat gagttaaccg
      301 gaggtgtttt atgtttttct atctatttat tgttatccaa tagtttttca aaagattttt
      361 ttatacttca accttcagat tgggtttatt tgttgattct gagttcgata tgtactgcat
      421 atgcttttat tgcatcggta agagtaatga agtttataag tccttacaca gtaatgctta
      481 ccataaattt agaacctgtt tatggaatca tattggcgtt attaattttt aaagacaaag
      541 aaaaaatgag ttctgaattt tattttggag ccattttgat attaatcact gtcattttaa
      601 atggagttat taaatataaa cttagaaata ggaatagttc ctagtgattt caatttccaa
      661 tcttctaaaa atgtttaaaa gttaacatta aaaataactt ttcttaataa gataaaattc
      721 tatctttgct tttcaaacaa acttaaattt atcaaaacct atggaatatt tagattttga
      781 acttcccatt aaagagttgg aagatcaatt ggacaaatgc caaattattg gtgaagaatc
      841 taatgttgat gttgcctcta cttgcaaaca aattgagaaa aaattagaag aaactaagaa
      901 aaaaatatac aaaaatcttt ctgcttggca acgggttcag ctttcccgtc atccaagtcg
      961 tccctatacg atggatcatg taagagcttt gtgcgggaat acttttctag aactttttgg
     1021 ggatagaaat tttaaagatg acaaagcaat gattggtgga ttaggaaaaa ttggtggaca
     1081 atcttttatg attgttggtc aacaaaaagg attcaatacc aaaacacgtc aatttagaaa
     1141 ttttggaatg gccaatcctg aagggtatcg aaaagctctt cgtttgatga aaatggctga
     1201 aaaattcggt attcctgtag ttactttgat tgatactccc ggagcttatc caggtttaga
     1261 agcagaagaa cgtggacaag gagaagccat tgctagaaat attctggaaa tgattcgatt
     1321 aaaaacacct attattaccg taattgtggg ggaaggagct tcaggtggag cattaggaat
     1381 tggggtagga gataaggttt atatgttaga aaacacttgg tattcagtca tttcaccgga
     1441 atcttgttcc tctattttgt ggagaagttg ggaatataaa gaacaagcgg ctgaagcact
     1501 taaattgact tcctatgaca tgaaaaagca aaagttaatt gatgaaattg tgccagaacc
     1561 attgggcgga gctcattatg acagagaaac tacatttaaa acagtagaac aatatattat
     1621 ca
//
LOCUS       DAOTCW010000278         1617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_69855_length_1617_cov_32.232394, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000278 DAOTCW010000000
VERSION     DAOTCW010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69855_length_1617_cov_32.232394"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..>1617)
                     /locus_tag="P5335_12305"
     CDS             complement(95..>1617)
                     /locus_tag="P5335_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRZ75709.1"
                     /translation="GWYENEMLNCVERLLDSHIKNGNGNNPCLRTFKKTWTYQDLFEK
                     ANQIAHVIVDDLGLQSGNRVLIRSANNPMMVALWFGILKAGGIVVATMPLLRSKELTT
                     IIDCAEISHVFCDIALEEEMNHVHSDFLKRVCKFCGSESGTSELENLAMNKPKTFDNF
                     HSKSDSVALIGFTSGTTGLPKMTAHYHKDILNSCEAFPNYSLQPTPNDIFTGSPPLGF
                     TFGLGGLVLFPLYFGASTFLIEKPTPDTLLEAIQEHKITICFTAPTAWRIITTKVKDF
                     DIASLRKCVSAGETLPLKVWEDWHNATGLKIIDGIGATEMLHIFISSNEENMKPGATG
                     VAITGYEAKIIDANGNEVSRNESGRLAVRGITGCKYLNRTEKQQEYVQNGWNITGDIF
                     KQDEDGYFWFVARGDDMIISSGYNIAAIEVESVLLTHEEILECAVVGLPDDERGMLVC
                     AHIVLKDISKATDNMKNHIQHWFKEVAAPYKYPRVIYFTEVLPKTETGKIQRFKLK"
BASE COUNT          476 a          333 c          283 g          525 t
ORIGIN      
        1 acacaatacc agcgtagtcg atgtgtttga attttatttt ttcttgtttg gtgaaaatca
       61 tactatttgt aatatcttaa tacttaatac ttcattactt caatttaaac ctttgtattt
      121 ttcctgtctc cgttttaggt aatacttccg taaaataaat cactcttggg tatttatacg
      181 gagcagctac ttctttgaac cagtgttgaa tatggttttt catattatct gttgctttag
      241 atatatcttt tagaacaata tgagcacaaa ccaacatgcc acgttcgtca tcgggtaaac
      301 ccactacggc acattccaaa atttcttcgt gggttaaaag tacggattct acttcaattg
      361 ccgcaatatt ataaccggaa gaaatgatca tatcatcgcc tcgagccaca aaccaaaagt
      421 aaccgtcttc atcttgtttg aaaatatctc cggtaatgtt ccaaccgttt tgaacgtatt
      481 cttgctgttt ctcagtccga ttcaagtatt tacagcccgt gattccgcga actgctaaac
      541 ggccggattc atttctggaa acttcattac cattcgcatc aataattttt gcttcatatc
      601 cggtaatcgc aactccggtg gctccgggtt tcatgttttc ttcgtttgaa gaaatgaaaa
      661 tatgtaacat ttcagttgct ccaatgccgt caatgatttt tagtccggtt gcattgtgcc
      721 aatcttccca cacttttaat ggcaaggttt ctccggcaga gacacatttg cgtaagctgg
      781 caatatcaaa gtcttttact tttgtagtga tgattcgcca agccgttgga gctgtaaaac
      841 aaatagtaat tttatgttcc tgaatagctt ccaataaagt atccggtgta ggtttttcga
      901 ttaaaaaagt agaagctcca aaatacaaag gaaacaaaac caatccaccc aaaccaaagg
      961 taaaacccag tggcggacta ccggtaaaaa tatcatttgg tgtgggttga agcgaatagt
     1021 ttggaaacgc ttcacaacta ttcaaaatat ctttgtggta atgagccgtc attttaggca
     1081 aacccgtcgt tccggaagta aatccgatta aggcaacact atctgatttt gagtgaaaat
     1141 tatcaaacgt tttgggtttg ttcattgcta agttttccaa ttcggaagtg ccactttcag
     1201 aaccacaaaa tttgcagact ctttttaaga aatcggaatg cacgtgattc atttcttctt
     1261 ccaatgcaat gtcacaaaac acgtgggata tttcggcaca gtcgatgata gttgttaatt
     1321 ctttcgaacg caacaaaggc atggttgcaa cgacaattcc acccgctttt aaaattccaa
     1381 accataacgc caccatcatt gggttattcg cagaccgaat tagaacgcgg tttccggatt
     1441 gcaaacctaa gtcatctact ataacatgag caatttgatt ggctttttca aataaatcct
     1501 gatacgtcca cgtttttttg aaggttcgaa gacaaggatt gtttccatta ccgtttttaa
     1561 tgtgagaatc taaaagacgt tcgacacaat tgagcatttc gttctcatac caacctt
//
LOCUS       DAOTCW010000279         1592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_71662_length_1592_cov_40.433312, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000279 DAOTCW010000000
VERSION     DAOTCW010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71662_length_1592_cov_40.433312"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..797
                     /locus_tag="P5335_12310"
     CDS             33..797
                     /locus_tag="P5335_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRZ75710.1"
                     /translation="MEQRFKKEGKYTYFESGEGIPIIVLHGLMGGLSNFGGVADFFPK
                     HGYKVVIPELPLYTQNILKTNVKAFARFVKDFITFKGFDKVILLGNSLGGHVALYHTK
                     MYPEKVIGMVLTGSSGLYESAMGDTYPKRGDYEYIKLKTQGVFYDPEMATKEVVDEVF
                     NIVNDRIKVIKTLTIAKSAIRHNMAKDLPKMTIPTCLIWGKNDKVTPPEVAEEFHKLL
                     PNSDLYWVDKCGHAAMMEHPTEFNHILLEWLQKTKF"
     gene            836..1453
                     /gene="yihA"
                     /locus_tag="P5335_12315"
     CDS             836..1453
                     /gene="yihA"
                     /locus_tag="P5335_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRZ75711.1"
                     /translation="MKITTAEFIISNSEVSKCPKDPLPEYAFIGRSNVGKSSLINMLT
                     NHKSLAKTSSKPGKTQLINHFKINNNWFLVDLPGYGYAKVSKKSKDVFQKFVTDYFEK
                     REQLVCAFVLIDIRLEAQKIDLEFITYLGVTEVPFCIIFTKADKIGKTRIQSHITAYR
                     KQVLASGWEEMPQHFVTSATEMTGKEELLSFIDNINQEMFKNNLL"
BASE COUNT          577 a          238 c          268 g          509 t
ORIGIN      
        1 tatttgtgag aaatagaaag aggtcatttt aaatggaaca acgttttaaa aaagaaggaa
       61 agtatactta ttttgaatcg ggagaaggca ttccgataat tgttttgcat ggtcttatgg
      121 gaggattaag taattttggc ggagtagctg atttttttcc gaaacatggg tacaaagttg
      181 tgattcctga acttccttta tacactcaaa acattttaaa aaccaatgtg aaagcctttg
      241 caagatttgt aaaagatttt atcactttca aaggatttga caaagtcatt ttattaggca
      301 attccttggg tggacatgtc gctttatacc atacaaaaat gtatcctgaa aaagttattg
      361 gcatggtact aacaggaagt agcggtttat atgaaagtgc catgggtgat acctatccaa
      421 aacgtggtga ttatgaatac attaaactaa aaacgcaagg tgttttttat gacccggaaa
      481 tggccacaaa agaggtagtt gatgaagtat ttaatattgt taacgaccgt attaaagtaa
      541 tcaaaacact tacaattgct aaaagtgcta ttcgtcataa tatggcaaaa gatttaccta
      601 aaatgactat tccaacttgc cttatttggg gtaaaaatga caaagtaacc ccaccggaag
      661 ttgcagaaga atttcataaa ttacttccaa attcagattt atattgggta gataaatgtg
      721 gtcatgccgc tatgatggaa catcctactg aatttaacca tatattatta gaatggttgc
      781 aaaaaactaa attttaaaaa gtccgaatgg acttttttgt ttttaaatta atcctatgaa
      841 aattacaaca gccgaattta tcatcagtaa ttctgaagta agtaaatgtc cgaaagaccc
      901 tttaccggaa tatgctttta ttggtcgttc caatgttgga aaatcatctt tgattaacat
      961 gcttacaaat cataaaagct tggcaaaaac ctcttcaaaa ccagggaaaa cacagcttat
     1021 taatcatttc aaaatcaata ataattggtt tttggttgat ttacccggtt atggttatgc
     1081 taaagtttcc aaaaaatcaa aagatgtatt tcagaaattt gtaaccgatt attttgagaa
     1141 aagagaacaa ttagtttgtg cttttgttct aatagacatc cgactcgaag ctcaaaaaat
     1201 cgatttggaa tttatcactt atttgggcgt gacggaagtt cctttttgca tcatttttac
     1261 caaagcagat aaaataggaa aaacaagaat tcaatcgcat atcacggctt acagaaaaca
     1321 agttttagcc agtggctggg aagaaatgcc gcaacatttt gtaacctctg caactgaaat
     1381 gacgggaaaa gaagaattac tttcttttat tgataatatc aatcaagaaa tgtttaaaaa
     1441 caatttatta taaaaaaagg ttagcaaatg ctaacctttt tttataaaga aaaagtttca
     1501 cttaattatg tgtttttaat tctaatttga aaaatccggc atcttccata atatttccaa
     1561 cctcatcatt ccaaaaaaca aatgactcat ta
//
LOCUS       DAOTCW010000280         1561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_74000_length_1561_cov_31.650066, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000280 DAOTCW010000000
VERSION     DAOTCW010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74000_length_1561_cov_31.650066"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="P5335_12320"
     CDS             <1..413
                     /locus_tag="P5335_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRZ75712.1"
                     /translation="DEAVQWTENNVNTNQNFFRSPNVYGNLMTTFSPKKAFQNNLSLV
                     YTGSMYVPHFAGYITTDTLEKTDHFFEVNIKSSYVFDAKEKFQIQLSGGIQNILNSYQ
                     SDFDKGVDRDASYIYGPSRPRTFFIGLKFGTDLL"
     gene            512..922
                     /locus_tag="P5335_12325"
     CDS             512..922
                     /locus_tag="P5335_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HRZ75713.1"
                     /translation="MYPEEMVKPMRAELSDVGFQELYSAEAVETALKQKGTTLVVVNS
                     VCGCAARNARPGAKMSLDGAKKPDQLVTVFAGVDKDAVDAARQHMFPFPPSSPSMALF
                     KDGELVHMLERHHIEGRPAEMIAENLKDAYNEFC"
     gene            complement(963..>1561)
                     /locus_tag="P5335_12330"
     CDS             complement(963..>1561)
                     /locus_tag="P5335_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRZ75714.1"
                     /translation="NPADFFTKVMPHIEYVTGGLDDIGKAMESAFFHIGNDAGITHVA
                     GAFNVPTVGIYGPTGPGSWGSFAQFNEIIWGKKGNCDLRCNYDVILNCPDRVCLSSTT
                     VPRVLNALYSLLQKAYEDIAFDFIVNPLLEVDFSETDCLLKIVETELLIEYRDLSMKN
                     SVEEILKGNFKENYSEEEEELIHVFIEQQVIFCIPELK"
BASE COUNT          528 a          279 c          269 g          485 t
ORIGIN      
        1 atgatgaagc tgttcaatgg acagaaaaca atgtgaatac gaatcagaat tttttccgtt
       61 caccaaatgt ttacggaaat ttaatgacta ctttttctcc taaaaaagct tttcaaaata
      121 atttgtcgtt ggtttacacc ggaagtatgt atgtccccca ttttgcagga tacattacaa
      181 cggacacttt ggaaaaaacc gatcactttt ttgaagtaaa tatcaaatcg tcatatgtgt
      241 ttgatgcgaa agaaaaattt caaattcagt taagtggggg tattcaaaac attttaaaca
      301 gttatcaaag tgattttgac aaaggggttg accgcgatgc aagttatatt tacggtccgt
      361 cacgacccag aacctttttt attggattga aatttggcac tgatttgttg taaaactcaa
      421 ttgaaccata acaaatcttt atgattataa tcattctaaa taattgaatt cttagtaact
      481 ttgcaatctg aaacttttaa aataaaagaa tatgtatcca gaagaaatgg taaaacccat
      541 gagagccgaa ttatcagacg ttggttttca ggaattatat agtgcagaag cggtagaaac
      601 tgctttaaaa caaaaaggaa ctacacttgt ggttgtgaat tcggtttgcg gttgtgccgc
      661 gagaaatgca agaccgggag caaaaatgag tttagatggt gcaaaaaaac cagaccaatt
      721 agtgactgtt tttgcaggtg ttgacaaaga tgctgttgat gccgccagac aacatatgtt
      781 tccttttcct ccttcatcac caagtatggc tttgtttaaa gatggtgaat tggttcatat
      841 gttagaacgc caccacattg aaggtcgccc ggcagaaatg attgctgaaa atttaaaaga
      901 tgcttataac gagttttgtt aagctaaaaa atcctttccg agaacaaatc agaaaggatt
      961 ttttatttta actcaggaat acaaaaaatt acttgttgtt ctataaaaac atgtatcaac
     1021 tcttcttctt cctctgaata attttctttg aaatttcctt ttaaaatttc ttcaacagaa
     1081 tttttcatcg ataaatctct gtattcaatt aacaattccg tttcaactat tttcaacaga
     1141 caatctgttt ctgaaaaatc aacttctaaa agtggattca ctataaaatc aaaagcgata
     1201 tcttcatatg ctttctgtaa aagtgaatac aacgcattta aaacgcgagg aactgttgtg
     1261 gaagacaaac aaacccgatc cgggcaattt agaataacat cataattgca tcgcaaatca
     1321 caattgcctt ttttacccca aataatctcg ttaaactgag caaaacttcc ccacgaacca
     1381 ggtccggttg ggccgtaaat tccaaccgtt ggcacattaa acgctccggc tacatgagta
     1441 attccggcgt catttccaat atggaaaaaa gcggattcca ttgcttttcc aatatcatct
     1501 aaaccgccgg tgacatattc aatatgaggc attacttttg taaaaaaatc ggccggattt
     1561 g
//
LOCUS       DAOTCW010000281         1545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_75227_length_1545_cov_39.605369, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000281 DAOTCW010000000
VERSION     DAOTCW010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75227_length_1545_cov_39.605369"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1283)
                     /locus_tag="P5335_12335"
     CDS             complement(123..1283)
                     /locus_tag="P5335_12335"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRZ75715.1"
                     /translation="MIRILHFVLLFSTFGISAQSFEIGRTTITFIDASRGNRSIASEI
                     YYPANSAGINVPVVSTTSESFPVLSFGHGFLMSWDAYQNIWEALVPQGYIMIFPKTEG
                     GASPSHLEFGKDLAFVINQMSLLNLNSSSIFYNRISDKSALMGHSMGGGAAFLAVQFS
                     NEIDAILTLAAAETNPSAIAAAGNLTIPALVIAGTNDCVTPTNSNQLPMYNALNSECK
                     SYISITGGSHCQMANYNLFCSIGESTCSPSPTISRTEQHQLLNPSMLYWLDYQLKSNC
                     SAGNSFQQLLNSSVGISTINNCEYCALNILELNESELFKAYPNPFQNEIRISAFPDQL
                     LKIECYNMHAELVFKETISAFTRINTEDWASGIYFIKCTDQKHKVSVFKLVK"
     gene            complement(1321..>1545)
                     /locus_tag="P5335_12340"
     CDS             complement(1321..>1545)
                     /locus_tag="P5335_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRZ75716.1"
                     /translation="HNILKGTTFTKYKQFVDNCFKVLPRQALHAKTLGFEHPITKEFL
                     RFDTEIPQDMAECVEKWRTYSKSHTLEEEE"
BASE COUNT          530 a          239 c          253 g          523 t
ORIGIN      
        1 aaataaaaat actgctgaga caagaacttc cgggaattca attcgaagag gttaaacttt
       61 aaattaataa aaacacaaag tagcgacttc aaaattcgct acttttttta tttaatttat
      121 ttttatttta ctaatttaaa tacagagact ttatgttttt gatctgtaca ttttataaaa
      181 taaattccag atgcccaatc ttcagtgtta attcttgtaa atgcagaaat agtttctttg
      241 aatactaatt ctgcatgcat attataacat tctattttta gcaattgatc aggaaaggct
      301 gaaattctta tttcattttg aaatggattt ggataggctt taaataactc actttcattt
      361 aattccaaaa tattaagtgc acaatattca caattattaa ttgtagagat tccaacggaa
      421 gagttaagta attgttgaaa tgaatttccg gcactacaat ttgatttcaa ttgataatca
      481 agccaatata acatgcttgg gtttaataat tgatgttgtt cagttctaga aatggtcggt
      541 gaaggcgaac aagtggattc tccaatgcta caaaacaaat tatagtttgc catttgacaa
      601 tgacttcctc cggtaatact tatatacgat ttgcattcac tattaagtgc attgtacatt
      661 ggtaattgat tggagtttgt tggagttaca caatcatttg ttccggcaat aaccaaagca
      721 ggaattgtta aatttcctgc tgctgcaata gctgaaggat tagtttctgc cgctgctaaa
      781 gtaagaattg catctatttc gtttgaaaat tgaacagcta aaaaagcagc tccgcctccc
      841 atactatgcc ccatcaaagc acttttatct gatatgcgat tgtaaaaaat agaagaggaa
      901 tttaaattta atagtgacat ttgatttatc acaaaggcta aatctttacc aaattctaaa
      961 tgagaaggag aagctcctcc ttctgttttt ggaaaaatca tgatgtagcc ttggggtact
     1021 aatgcttccc aaatattttg ataagcatcc caggacatta aaaatccatg cccaaagctc
     1081 aaaaccggaa aagattcaga agttgtagaa acaacaggaa cattaattcc tgcggagttg
     1141 gctggataat aaatttcaga ggcgatagaa cgatttcctc gactggcatc aatgaaagta
     1201 atggtagttc tacctatttc aaatgattga gccgatattc caaaagttga aaaaagtaat
     1261 acaaaatgta aaattctaat cataatttaa actttttaag cgtttaaata taataaaaaa
     1321 ttactcttct tcttccaaag tatgtgattt tgaataagtt cgccattttt caacacattc
     1381 ggccatatct tgtgggattt cagtgtcaaa gcgtaaaaat tctttggtta ttggatgttc
     1441 aaaacctaaa gtttttgcat gaagagcttg tcggggcaat actttaaaac aattgtctac
     1501 aaactgtttg tattttgtga aagttgtccc tttaagaatg ttatg
//
LOCUS       DAOTCW010000282         1525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_3171
            NODE_76787_length_1525_cov_39.383673, whole genome shotgun
            sequence.
ACCESSION   DAOTCW010000282 DAOTCW010000000
VERSION     DAOTCW010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868195
            Sequence Read Archive: SRR5007150
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:51:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,434
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76787_length_1525_cov_39.383673"
                     /isolate="AMR_MDS_3171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2015-01-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="P5335_12345"
     CDS             complement(<1..468)
                     /locus_tag="P5335_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRZ75717.1"
                     /translation="MNDAHLHMVVNHFPIIGTIFGLGILIAGLIFKNTATKNVAYVLF
                     VVAAIFAAFSMGTGEGAEEIAEKLPDVTDQIIHEHEEMAEKLALVLYALGAFSLVGLF
                     LNFVKNSNEKFVSYLAVVIAAVAVFLAKQTGTTGGEIRHTEIRANATTAGVNQN"
     gene            complement(517..>1525)
                     /locus_tag="P5335_12350"
     CDS             complement(517..>1525)
                     /locus_tag="P5335_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HRZ75718.1"
                     /translation="SLAEIENGHAIHMLNKVKEVESNYTMPSPSSRAKFQLKLGKIFG
                     YTSIISNLSSIEKQFAVNTIKNKIISGEKITGFEHNHLKIIEAVNNNAALNVSGGFLS
                     KFESRHKSVGGNALRAAVLGANDGLVSNMSLVMGVAGAAVSNNTILLTGIAGLLAGAI
                     SMALGEWLSVQSSRELHLRQIELETEELEASPEEEKKELILLYQAKGMTPFEAKKLAE
                     KAFVTNESAIEALIVEELGIDKEELGGSAWEAAITSFVLFAIGAIIPLFPFFILKGYN
                     AIYMSVGASVVGLFGIGAAITLFTGKGVFYSGMRQVFFGLAAAAVTFGIGTLIGVSLA
                     G"
BASE COUNT          457 a          328 c          255 g          485 t
ORIGIN      
        1 attttgattc acgccggcag tagttgcatt agctctaatt tccgtatgac gaatttctcc
       61 tccggtagtt ccggtttgtt tagcaagaaa aaccgccacc gcagcgataa caacagccaa
      121 gtaggaaaca aatttttcgt ttgaattctt aacgaaattt agaaataaac ccactaatga
      181 aaatgcccct aaggcataga gtacaagggc taatttttct gccatttctt cgtgttcatg
      241 aattatttgg tcggttacat ccggaagctt ttcagcaatt tcctccgctc cttcccctgt
      301 tcccatacta aaagcggcaa agattgctgc taccacaaaa agtacatagg caacgttttt
      361 agttgcggta tttttaaaaa ttaatccggc aattaaaatg cctagtccaa aaatcgtccc
      421 gataattgga aagtggttca ccaccatgtg taaatgtgcg tcgttcatta tttctattgt
      481 ttaaaggttt aaagtcgttt aaaagtttaa ggttgtttag ccggctaatg aaactccgat
      541 taatgttcca attccaaagg taacagctgc cgctgctaag ccaaaaaaca cctgacgcat
      601 accggaataa aaaacacctt ttccggtgaa taacgtgatg gctgcaccaa ttccgaatag
      661 acccacaaca cttgcaccca cactcatata aatagcgttg taccctttta aaataaaaaa
      721 gggaaaaagt ggtataatgg caccaatggc aaataaaacg aaagaagtta ttgccgcttc
      781 ccatgcagaa ccacccaatt cttctttatc aattccaagt tcctctacaa ttaatgcttc
      841 tattgccgat tcattcgtta caaaagcttt ttctgctaat ttttttgctt caaaaggagt
      901 cattcctttg gcttggtata ataatattaa ttcttttttt tcttcttcgg gcgaggcttc
      961 caattcttcc gtttctaatt caatttgacg caaatgcaat tcgcgtgagc tttgtacaga
     1021 aagccattcg cccaaagcca ttgaaatagc tcccgccaaa agtcctgcaa taccggttag
     1081 taaaatagta ttgtttgaaa ctgccgcacc ggcaaccccc atcactaaac tcatattaga
     1141 aactaaccca tcattcgcac caagaactgc agctctaagg gcgttccctc ctactgattt
     1201 atgtctactt tcaaatttag agagaaatcc acctgaaaca tttaatgctg cattgttatt
     1261 gaccgcttca attattttga ggtgattgtg ttcaaaaccg gtaatctttt cacctgatat
     1321 aattttattt ttaatggtat tgactgcaaa ttgtttttca atgcttgaaa ggttgctgat
     1381 gatggaagta tatccgaaaa tcttacctaa tttcaattga aattttgctc ttgaagaagg
     1441 cgatggcatg gtataattcg attctacttc ttttacttta ttaagcatgt gaatcgcatg
     1501 tccgttttca atttcagcca aactt
//
